From cbass <@t> wfubmc.edu Thu May 1 07:43:33 2008 From: cbass <@t> wfubmc.edu (Caroline Bass) Date: Thu May 1 07:55:54 2008 Subject: [Histonet] MRNA stability in brain section Message-ID: Hello, I?m trying real time RT-pcr with samples from brain sections. I have injected a virus into the striatum of a rat which among other things expresses GFP. I?d like to collect fresh brain, and cut a rough 5-mm slice around the injection site (around where the needle went in). Ideally I?d freeze this on dry ice to keep the mRNA safe, and at a later time point cut large sections (200-300 microns) using an AO 860 sliding microtome. I can drop these immediately in buffer, visualize the GFP under the microscope and roughly dissect out the transduced region under the scope. I could hand dissect the tissue, but it will dilute the effect of the virus since the entire region is not affected. So, my question is, how reasonable is this approach? In particular, can does the initial dry ice freezing-thawing during section and dissection hurt the mRNA? How quick do I have to be to prevent loss? Is there a particularly good buffer to prevent Rnase activity (autoclaved PBS?)? Perhaps most importantly, will I be able to cut thick sections from an unfixed brain on the sliding microtome? Thin sections from unfixed brain tend to fall apart. I know this isn?t the ideal system, but I?m trying to work with the equipment I have. An alternative would be to take a quick section, look under the scope and then do a rough dissection of the frozen mounted brain once I start to see a signal. Thanks, Caroline From bakevictoria <@t> gmail.com Thu May 1 08:58:21 2008 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Thu May 1 08:58:26 2008 Subject: [Histonet] Old Recycler In-Reply-To: References: Message-ID: <4f016b690805010658h2b2a66dakaaf32c92f52565d@mail.gmail.com> I'd contact your Safety and Maintenance departments to co-ordinate the removal of a hazardous materials instrument. It's a hazmat type issue that Safety should be well versed with The instrument will, most likely, have to be 'decontaminated' before they can break it down and discard. Recommended line of contacts for this would be your immediate supervisor and the Safety department supervisor. Do all of this in a written format that you have documentation to follow up on and keep for record purposes. OSHA/NIOSH have good websites to look at as well. I like John's method too though ;-) To quote PT Barnum - "There's a sucker born everyday!" Vikki On 4/30/08, Crystal Morris wrote: > Does anyone know how I should go about disposing of an old xylene recycler that no longer works? > Thanks > Crystal > > "Confidentiality Notice: This e-mail message, including any > attachments is for the sole use of the intended recipient(s) > and may contain confidential and privileged information. > Any unauthorized review; use; disclosure or distribution > is prohibited. If you are not the intended recipient, > please contact the sender by reply e-mail and destroy all copies of > the original message." > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From victor <@t> pathology.washington.edu Thu May 1 09:13:19 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Thu May 1 09:13:24 2008 Subject: [Histonet] Old Recycler In-Reply-To: <4f016b690805010658h2b2a66dakaaf32c92f52565d@mail.gmail.com> References: <4f016b690805010658h2b2a66dakaaf32c92f52565d@mail.gmail.com> Message-ID: <4819CFFF.2050907@pathology.washington.edu> Will the vendor take it off your hands to possibly refurbish? Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Victoria Baker wrote: > I'd contact your Safety and Maintenance departments to co-ordinate the > removal of a hazardous materials instrument. It's a hazmat type issue > that Safety should be well versed with The instrument will, most > likely, have to be 'decontaminated' before they can break it down and > discard. Recommended line of contacts for this would be your > immediate supervisor and the Safety department supervisor. Do all of > this in a written format that you have documentation to follow up on > and keep for record purposes. OSHA/NIOSH have good websites to look > at as well. > > I like John's method too though ;-) To quote PT Barnum - "There's a > sucker born everyday!" > > Vikki > > > > > > On 4/30/08, Crystal Morris wrote: > >> Does anyone know how I should go about disposing of an old xylene recycler that no longer works? >> Thanks >> Crystal >> >> "Confidentiality Notice: This e-mail message, including any >> attachments is for the sole use of the intended recipient(s) >> and may contain confidential and privileged information. >> Any unauthorized review; use; disclosure or distribution >> is prohibited. If you are not the intended recipient, >> please contact the sender by reply e-mail and destroy all copies of >> the original message." >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From HornHV <@t> archildrens.org Thu May 1 09:29:47 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu May 1 09:30:09 2008 Subject: [Histonet] tb in bal Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82C20@EMAIL.archildrens.org> We are expecting a positive TB bronch lavage. We do not have cytofix and the microbiology department puts bleach in the specimen to kill the TB. What will this do to our stains. Probably a Dif Quik and an AFB. Would 95% kill TB to fix the specimen? HELP! Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From victor <@t> pathology.washington.edu Thu May 1 09:36:45 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Thu May 1 09:36:51 2008 Subject: [Histonet] PowerPath Users Conference Message-ID: <4819D57D.8050406@pathology.washington.edu> The conference is in Philadelphia next week and I was curious if users are going. It would be nice to put a face with a name as I don't go to NSH conventions any more. Victor -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From annigyg <@t> gmail.com Thu May 1 10:23:43 2008 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Thu May 1 10:23:47 2008 Subject: [Histonet] tb in bal In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82C20@EMAIL.archildrens.org> References: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82C20@EMAIL.archildrens.org> Message-ID: surely micro can spare an aliquot for you before they 'decontaminate' the BAL how on earth do micro do TB cultures if they flood the specimen with bleach!!! we have lots of TB+ve BALS and HIV+ve ones too - we handle them all in a biohaz cab and wear all the necessary garb - quite safe we dont use cytofix and we certainly do not mix with bleach just relax - you will not get TB if you follow universal precautions Annie in Arabia (out of Africa) 2008/5/1 Horn, Hazel V : > We are expecting a positive TB bronch lavage. We do not have cytofix > and the microbiology department puts bleach in the specimen to kill the > TB. What will this do to our stains. Probably a Dif Quik and an AFB. > Would 95% kill TB to fix the specimen? HELP! > > > > Hazel Horn > > Hazel Horn, HT/HTL (ASCP) > > Supervisor of Histology > > Arkansas Children's Hospital > > 800 Marshall Slot 820 > > Little Rock, AR 72202 > > > > phone 501.364.4240 > > fax 501.364.3155 > > > > visit us on the web at: www.archildrens.org > > > > > ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > The information contained in this message may be privileged and > confidential > and protected from disclosure. If the reader of this message is not the > intended recipient, or an employee or agent responsible for delivering > this > message to the intended recipient, you are hereby notified that any > dissemination, distribution or copying of this communication is strictly > prohibited. If you have received this communication in error, please > notify > us immediately by replying to the message and deleting it from your > computer. > Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne (van Binsbergen) Hope Abu Dhabi UAE From jerry.santiago <@t> jax.ufl.edu Thu May 1 11:47:33 2008 From: jerry.santiago <@t> jax.ufl.edu (Santiago, Jerry) Date: Thu May 1 11:47:43 2008 Subject: [Histonet] Telomerase Antibody Message-ID: <816DC61E1730E843A0BCF4CCFA1EFCF30148F23D@jaxmail.umc.ufl.edu> Hello netters, Is anyone doing telomerase by IHC with success? Various companies have discontinued this companies, so we are searching around for anyone having success with this antibody. Thanks, Jerry Santiago, B.S., HTL(ASCP)QIHC Pathology Technologist Shands Jacksonville (904) 244-6149 E-mail: jerry.santiago@jax.ufl.edu From gu.lang <@t> gmx.at Thu May 1 11:48:18 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu May 1 11:48:25 2008 Subject: [Histonet] p16ink4 Message-ID: <000901c8abab$29091ec0$eeeea8c0@dielangs.at> Hi listmembers, I am trying to find a vendor for p16ink antibody for cervical cancer diagnostic. Is it possible, that p16 is only selled by one firm called mtmlab now? It looks like they have the patent on the antibody. Or is there any other resource? I need "only" the antibody concentrate and not a whole kit to run it on the Ventana benchmark. I found a p16 on the Ventana website, but cannot see, if it is for the same purpose. Any help is appreciated. Gudrun Lang From settembr <@t> umdnj.edu Thu May 1 11:58:44 2008 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Thu May 1 11:59:16 2008 Subject: [Histonet] p16ink4 Message-ID: Gudrun, I believe you are correct mtmlab has a patent and is supposed to be the only people that sell it. Cell Marque was given permission to sell what they had by a certain time, I think. I too only need the antibody and not the whole kit but they have you buy their whole kit and it is expensive. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Gudrun Lang 05/01/08 12:48 PM >>> Hi listmembers, I am trying to find a vendor for p16ink antibody for cervical cancer diagnostic. Is it possible, that p16 is only selled by one firm called mtmlab now? It looks like they have the patent on the antibody. Or is there any other resource? I need "only" the antibody concentrate and not a whole kit to run it on the Ventana benchmark. I found a p16 on the Ventana website, but cannot see, if it is for the same purpose. Any help is appreciated. Gudrun Lang From anh2006 <@t> med.cornell.edu Thu May 1 12:25:16 2008 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Thu May 1 12:27:40 2008 Subject: [Histonet] p16ink4 In-Reply-To: References: Message-ID: <48175415-1209662855-cardhu_decombobulator_blackberry.rim.net-712165279-@bxe115.bisx.prod.on.blackberry> Cell Signaling and Santa Cruz also sells p16 but I have no idea if it works for IHC. -----Original Message----- From: Dana Settembre Date: Thu, 01 May 2008 12:58:44 To:Gudrun Lang , histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] p16ink4 Gudrun, I believe you are correct mtmlab has a patent and is supposed to be the only people that sell it. Cell Marque was given permission to sell what they had by a certain time, I think. I too only need the antibody and not the whole kit but they have you buy their whole kit and it is expensive. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Gudrun Lang 05/01/08 12:48 PM >>> Hi listmembers, I am trying to find a vendor for p16ink antibody for cervical cancer diagnostic. Is it possible, that p16 is only selled by one firm called mtmlab now? It looks like they have the patent on the antibody. Or is there any other resource? I need "only" the antibody concentrate and not a whole kit to run it on the Ventana benchmark. I found a p16 on the Ventana website, but cannot see, if it is for the same purpose. Any help is appreciated. Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Thu May 1 12:37:38 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu May 1 12:37:44 2008 Subject: AW: [Histonet] p16ink4 - thanks In-Reply-To: <48175415-1209662855-cardhu_decombobulator_blackberry.rim.net-712165279-@bxe115.bisx.prod.on.blackberry> Message-ID: <002101c8abb2$0d23d680$eeeea8c0@dielangs.at> Thank you all for your answers. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von anh2006@med.cornell.edu Gesendet: Donnerstag, 01. Mai 2008 19:25 An: Dana Settembre; histonet@lists.utsouthwestern.edu Betreff: Re: [Histonet] p16ink4 Cell Signaling and Santa Cruz also sells p16 but I have no idea if it works for IHC. -----Original Message----- From: Dana Settembre Date: Thu, 01 May 2008 12:58:44 To:Gudrun Lang , histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] p16ink4 Gudrun, I believe you are correct mtmlab has a patent and is supposed to be the only people that sell it. Cell Marque was given permission to sell what they had by a certain time, I think. I too only need the antibody and not the whole kit but they have you buy their whole kit and it is expensive. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Gudrun Lang 05/01/08 12:48 PM >>> Hi listmembers, I am trying to find a vendor for p16ink antibody for cervical cancer diagnostic. Is it possible, that p16 is only selled by one firm called mtmlab now? It looks like they have the patent on the antibody. Or is there any other resource? I need "only" the antibody concentrate and not a whole kit to run it on the Ventana benchmark. I found a p16 on the Ventana website, but cannot see, if it is for the same purpose. Any help is appreciated. Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Thu May 1 13:09:52 2008 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Thu May 1 13:11:18 2008 Subject: [Histonet] Mouse anti-C5b-9 Message-ID: <4819CF30.2B7F.00C9.0@geisinger.edu> Is anyone using this antibody for distinction of lupus vs dermatomyositis, and would like to share their automated protocol? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From jmjohnson34 <@t> hotmail.com Thu May 1 13:28:53 2008 From: jmjohnson34 <@t> hotmail.com (Jennifer Johnson) Date: Thu May 1 13:28:57 2008 Subject: [Histonet] Pneumocystis controls Message-ID: I use Histology Control Systems, Inc for Pneumocystis controls. 1-800-253-2768 or www.HistologyControlSystems.com Also, thanks to everyone who gave me advice on the Bielschowsky stain. One drop of concentrated Hydrochloric acid did NOT yield a positive result. You really do need the ONE drop of concentrated Nitric Acid for the stain to work properly. Jennifer Johnson, HTL (ASCP) _________________________________________________________________ Make i'm yours.? Create a custom banner to support your cause. http://im.live.com/Messenger/IM/Contribute/Default.aspx?source=TXT_TAGHM_MSN_Make_IM_Yours From integrated.histo <@t> gmail.com Thu May 1 13:45:17 2008 From: integrated.histo <@t> gmail.com (Cindy DuBois) Date: Thu May 1 13:45:27 2008 Subject: [Histonet] Tea Bags Message-ID: <5d9104a30805011145l169a3c8m4c8f539c283ce7b2@mail.gmail.com> We bought the tea bags and so far have liked them better than the nylon bags for biopsies such as colon, esophagus & gastric. How are they for ECCs and EMB's? My concern is the ECC's are usually very scant and I am afraid what little there is may be absorbed by the paper in the tea bag. What has been your experience? In your experience are they okay to use for scant specimens? Cindy From leiker <@t> buffalo.edu Thu May 1 15:55:58 2008 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Thu May 1 15:56:07 2008 Subject: [Histonet] IF muscle cytoskeletal proteins - interior of myocytes doesn't stain Message-ID: Hi, I'm trying to stain hamster skeletal and cardiac muscle for Troponin I, Troponin T, and Myosin Heavy Chain (separately). When I do Z stack imaging at 0.5um steps, only the outermost myofibrils (near the sarcolemma) of most of the myocytes are stained; the interior (particularly of te larger myocytes) is unstained, looking like a gaping black hole. The tissues were snap frozen, cut 8um thick, fixed on the slide in 4%PFA, and permeabilized in 1% Triton. All antibody incubations were done at either 1 hr RT or overnight 4oC. The microscope used was Zeiss Axioimager (epifluorescent). Can you tell me if this is expected staining for these material and conditions, or if something is wrong? Thank you. Merced M Leiker Research Technician II 354 BRB (Lee Lab) / 140 Farber Hall (mail) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 From jcline <@t> wchsys.org Thu May 1 16:06:57 2008 From: jcline <@t> wchsys.org (Joyce Cline) Date: Thu May 1 16:07:03 2008 Subject: [Histonet] Paraffin types Message-ID: <000e01c8abcf$4a91d3b0$1d2a14ac@wchsys.org> What type of paraffin is everyone using? Separate paraffin for processing and embedding, or the same for processing and embedding? Paraplast, Surgipath, Shandon ???? ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From HMLacy <@t> uams.edu Thu May 1 17:25:17 2008 From: HMLacy <@t> uams.edu (Lacy, H Marie) Date: Thu May 1 17:25:28 2008 Subject: [Histonet] (no subject) Message-ID: <8520B38B62EC9B43991358FB6732346C408778@MAIL4.ad.uams.edu> Good day everyone, Does anyone have a protocol for the naphthol AS-D chloroacetate esterase stain for formalin fixed, paraffin-embedded animal tissue? I am trying to use this stain to identify and count PMNs in sections of guinea pig and mouse tissue. I have tried both Sigma kits for this stain (#90 and #91). They work well on human tissue but not on guinea pig or mouse (even after varying the pH and time). Your help will be greatly appreciated. Marie Lacy Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From gayle.callis <@t> bresnan.net Thu May 1 19:13:23 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Thu May 1 19:13:22 2008 Subject: [Histonet] Paraffin types References: <000e01c8abcf$4a91d3b0$1d2a14ac@wchsys.org> Message-ID: <001201c8abe9$561a8f50$6501a8c0@DHXTS541> Same paraffin for infiltration and embedding. Our choice is especially good for bone because it is a harder paraffin and works well for soft tissues even very thin sections i.g. 1 to 2 um. Since we do a great deal of bone work, this has been a good choice. Tissue Prep 2 from ThermoFisher Scientific. We have used the Surgipath combination infiltration media followed by their embedding media with great success. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Joyce Cline" To: Sent: Thursday, May 01, 2008 3:06 PM Subject: [Histonet] Paraffin types > What type of paraffin is everyone using? Separate paraffin for > processing and embedding, or the same for processing and embedding? > > Paraplast, Surgipath, Shandon ???? > > From annigyg <@t> gmail.com Fri May 2 01:59:19 2008 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Fri May 2 01:59:24 2008 Subject: [Histonet] Paraffin types In-Reply-To: <001201c8abe9$561a8f50$6501a8c0@DHXTS541> References: <000e01c8abcf$4a91d3b0$1d2a14ac@wchsys.org> <001201c8abe9$561a8f50$6501a8c0@DHXTS541> Message-ID: one size fits all - tissue-tek works for me - been using it for over 10 years now - in all the labs i have managed Annie 2008/5/2 Gayle Callis : > Same paraffin for infiltration and embedding. Our choice is especially > good for bone because it is a harder paraffin and works well for soft > tissues even very thin sections i.g. 1 to 2 um. Since we do a great deal of > bone work, this has been a good choice. Tissue Prep 2 from ThermoFisher > Scientific. > > We have used the Surgipath combination infiltration media followed by > their embedding media with great success. > > Gayle M. Callis > HTL/HT/MT(ASCP) > Bozeman MT 59715 > > > ----- Original Message ----- From: "Joyce Cline" > To: > Sent: Thursday, May 01, 2008 3:06 PM > Subject: [Histonet] Paraffin types > > > What type of paraffin is everyone using? Separate paraffin for > > processing and embedding, or the same for processing and embedding? > > > > Paraplast, Surgipath, Shandon ???? > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne (van Binsbergen) Hope Abu Dhabi UAE From pieronelva01 <@t> bigpond.com Fri May 2 06:12:27 2008 From: pieronelva01 <@t> bigpond.com (Piero Nelva) Date: Fri May 2 06:12:35 2008 Subject: [Histonet] Paraffin types References: <000e01c8abcf$4a91d3b0$1d2a14ac@wchsys.org><001201c8abe9$561a8f50$6501a8c0@DHXTS541> Message-ID: <004e01c8ac45$67c761e0$5c72be7c@pentium4> I've only ever used the same wax for both processes. Paraplast is the current (>10 years) favorite. Piero ----- Original Message ----- From: "Anne van Binsbergen" To: "Gayle Callis" Cc: Sent: Friday, May 02, 2008 4:59 PM Subject: Re: [Histonet] Paraffin types > one size fits all - tissue-tek works for me - been using it for over 10 > years now - in all the labs i have managed > Annie > > 2008/5/2 Gayle Callis : > >> Same paraffin for infiltration and embedding. Our choice is especially >> good for bone because it is a harder paraffin and works well for soft >> tissues even very thin sections i.g. 1 to 2 um. Since we do a great deal >> of >> bone work, this has been a good choice. Tissue Prep 2 from >> ThermoFisher >> Scientific. >> >> We have used the Surgipath combination infiltration media followed by >> their embedding media with great success. >> >> Gayle M. Callis >> HTL/HT/MT(ASCP) >> Bozeman MT 59715 >> >> >> ----- Original Message ----- From: "Joyce Cline" >> To: >> Sent: Thursday, May 01, 2008 3:06 PM >> Subject: [Histonet] Paraffin types >> >> >> What type of paraffin is everyone using? Separate paraffin for >> > processing and embedding, or the same for processing and embedding? >> > >> > Paraplast, Surgipath, Shandon ???? >> > >> > >> > >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > Anne (van Binsbergen) Hope > Abu Dhabi > UAE > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG. > Version: 7.5.524 / Virus Database: 269.23.7/1410 - Release Date: 5/1/2008 > 5:30 PM > > From Teri.Hallada <@t> midmichigan.org Fri May 2 07:14:11 2008 From: Teri.Hallada <@t> midmichigan.org (Teri.Hallada@midmichigan.org) Date: Fri May 2 07:14:24 2008 Subject: [Histonet] Ventanna Giemsa Message-ID: <8839B08E3ED7364E8CBBD53882C984D50994CC67@MAILSRV01.midmichigan.net> We are having trouble with our Ventanna Giemsa stain for bone marrows. The cells either fall off the slide or explode. Anyone out there willing to share there procedure or have any suggestions? Teresa Hallada BS, MT/CT (ASCP) Lead Cytotechnologist MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. From mpence <@t> grhs.net Fri May 2 08:18:42 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Fri May 2 08:18:50 2008 Subject: [Histonet] Ventanna Giemsa In-Reply-To: <8839B08E3ED7364E8CBBD53882C984D50994CC67@MAILSRV01.midmichigan.net> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A380D@IS-E2K3.grhs.net> I could never get this stain to work for us and we went back to manual staining. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri.Hallada@midmichigan.org Sent: Friday, May 02, 2008 7:14 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Ventanna Giemsa We are having trouble with our Ventanna Giemsa stain for bone marrows. The cells either fall off the slide or explode. Anyone out there willing to share there procedure or have any suggestions? Teresa Hallada BS, MT/CT (ASCP) Lead Cytotechnologist MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Weber2 <@t> va.gov Fri May 2 08:56:01 2008 From: Susan.Weber2 <@t> va.gov (Weber, Susan (VHACLE)) Date: Fri May 2 08:56:27 2008 Subject: [Histonet] Paraffin types In-Reply-To: <000e01c8abcf$4a91d3b0$1d2a14ac@wchsys.org> References: <000e01c8abcf$4a91d3b0$1d2a14ac@wchsys.org> Message-ID: <16C83872A53F4346AA9C3A18E3A3AAB903F76D92@VHAV10MSGA1.v10.med.va.gov> We are using Surgipath Blue Ribbon Tissue Embedding/Infiltration Medium. As the name implies, we use only this one paraffin for both. Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Thursday, May 01, 2008 5:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin types What type of paraffin is everyone using? Separate paraffin for processing and embedding, or the same for processing and embedding? Paraplast, Surgipath, Shandon ???? ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Fri May 2 10:31:33 2008 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri May 2 10:31:40 2008 Subject: [Histonet] DRS 60 jackpot! Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F568@lmhsmail.lmhealth.org> Rummaging around and low and behold.... I found 10-12 plastic staining dishes for the Sakura DRS 60! I know that some of these workhorses are still around and that you probably can't get dishes for them anymore, so.... Send me your fedex number and address and I'll ship them out to anyone who wants them. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org From schaundrawalton <@t> yahoo.com Fri May 2 10:48:53 2008 From: schaundrawalton <@t> yahoo.com (Schaundra Walton) Date: Fri May 2 10:48:57 2008 Subject: [Histonet] RE:Pneumocystis controls Message-ID: <661422.27682.qm@web58909.mail.re1.yahoo.com> We just recently ordered these controls slides from Mercedes Medical. [1]www.mercedesmedical.com We were having trouble getting slides from our former supplier and Mercedes' prices were pretty comparable. Hope this helps! Schaundra Walton BS, HTL(ASCP) Histology Supervisor Swedish American Hospital Rockford, IL -----Original Message----- Hoping someone knows of a supplier of pneumocystis TISSUE control slides? Thanks Nancy Rutledge _________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. [2]Try it now. References 1. http://www.mercedesmedical.com/ 2. http://us.rd.yahoo.com/evt=51733/*http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ From ROrr <@t> enh.org Fri May 2 11:01:28 2008 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Fri May 2 11:01:34 2008 Subject: [Histonet] IL-1 IL-6 Tnf alpha help Message-ID: I everyone, I have a researcher who is looking for a lab that will run these antibodies on 24hr Urines. I'm not sure how many samples he has, but if anyone has a reference lab they could recommend I'd be obliged. Mayo does some of these but only on blood/plasma. I'm thinking the specimen type will be the issue. Thanks and let's all have a great weekend. Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 From Masterson_John <@t> Allergan.com Fri May 2 11:30:57 2008 From: Masterson_John <@t> Allergan.com (Masterson_John) Date: Fri May 2 11:31:44 2008 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: <0C58C4F16F0B67448318A38041CADE4B01594E0B@IRMAIL133.irvine.allergan.com> -----Original Message----- Hi Histonetter's, Thanks to all who replied to my last question regarding M.O.M. IHC kits. Does anyone have any wisdom regarding the Bodian stain. I've purchased a kit from a well known vendor but after 4 or 5 attempts I still can't get the section to turn dark tan to brown as described for the protargol step. I've ordered a bottle of pre mixed 1% protargol from another vendor and it doesn't work any better. Any advice? Thanks, John From griz52 <@t> gmail.com Fri May 2 12:11:01 2008 From: griz52 <@t> gmail.com (Griz Willis) Date: Fri May 2 12:11:21 2008 Subject: [Histonet] Grossing Stations Message-ID: <797735aa0805021011i4cef928aga3cc0a8c3e19b760@mail.gmail.com> Hi, Is anyone familiar with either the CSI/Jewett JGP5 or ThermoShandon Gross-Star or Gross Senior elevating grossing workstations? We are having a new lab built and would like to hear any complaints or praises of any of these units. Thanks. Dan Harborview Medical Center Pathology Histology Supervisor From John.Spair <@t> multicare.org Fri May 2 12:25:10 2008 From: John.Spair <@t> multicare.org (John Spair) Date: Fri May 2 12:25:27 2008 Subject: [Histonet] RE: Histonet Digest, Vol 54, Issue 2 In-Reply-To: <640597752EO25268-01@MMS_multicare.org> References: <640597752EO25268-01@MMS_multicare.org> Message-ID: <61A9977919846C479389493BAE2517CA02275954@MHSEXMBX1.multicare.org> Re: Paraffin Types We're using Paraplast for processing and Paraplast Plus for embedding. Been using Paraplast for a good 20 years. Thank you all who answered my question about bone marrow fixative. Seems the majority of people in histo land here, are using the B+, which I'm going to try it out. Now another question. The pathologist's use Bouin's to dip their specimens in after inking the margins. I'd actually like to get rid of Bouin's from the gross room. I'm thinking what is setting ink/dye is really the acetic acid/water combination. Has anyone ever used just acetia acid/water, or white vinegar for that matter?? Thank you. John Spair, System Manager Pathology Services LABORATORIES Northwest Multicare Health System Tacoma, WA John.spair@multicare.org "MMS " made the following annotations. ------------------------------------------------------------------------------ NOTICE: This e-mail and the attachments hereto, if any, may contain privileged and/or confidential information. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, you are hereby notified that any examination, distribution or copying of this e-mail and the attachments hereto, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by email or telephone and permanently delete this e-mail and the attachments hereto, if any, and destroy any printout thereof. MultiCare Health System, Tacoma, WA 98415 (253) 403-1000. ============================================================================== From LuckG <@t> empirehealth.org Fri May 2 12:30:33 2008 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Fri May 2 12:30:38 2008 Subject: [Histonet] Paraffin types(new) In-Reply-To: <001201c8abe9$561a8f50$6501a8c0@DHXTS541> References: <000e01c8abcf$4a91d3b0$1d2a14ac@wchsys.org> <001201c8abe9$561a8f50$6501a8c0@DHXTS541> Message-ID: <6BB8BC4519AAB844B174FC739A679BBCCF00D3@IRMEXCH01.irm.inhs.org> Hello All, We use Formula "R" from Surgipath for infilitrating (cuz it's inexpensive) and Polyfin from PBS for embedding as it is a superb ribboning and semitransparent paraffin. A little softer than Tissue Prep 2 but superior in ribboning. Greg Luck, B.S., HT(ASCP) Anatomic Pathology Supervisor Deaconess Med Cntr 800 W. 5th Ave Spokane, WA 99204 Offc 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmc.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Thursday, May 01, 2008 5:13 PM To: Joyce Cline; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin types Same paraffin for infiltration and embedding. Our choice is especially good for bone because it is a harder paraffin and works well for soft tissues even very thin sections i.g. 1 to 2 um. Since we do a great deal of bone work, this has been a good choice. Tissue Prep 2 from ThermoFisher Scientific. We have used the Surgipath combination infiltration media followed by their embedding media with great success. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Joyce Cline" To: Sent: Thursday, May 01, 2008 3:06 PM Subject: [Histonet] Paraffin types > What type of paraffin is everyone using? Separate paraffin for > processing and embedding, or the same for processing and embedding? > > Paraplast, Surgipath, Shandon ???? > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Beatrice.Debrosse-Serra <@t> pfizer.com Fri May 2 12:42:15 2008 From: Beatrice.Debrosse-Serra <@t> pfizer.com (Debrosse-Serra, Beatrice) Date: Fri May 2 12:42:36 2008 Subject: [Histonet] (no subject) In-Reply-To: <0C58C4F16F0B67448318A38041CADE4B01594E0B@IRMAIL133.irvine.allergan.com> Message-ID: <8404DFBED5207B4B8E5EEF4332CEEA53071EA35B@lajamrexm01.amer.pfizer.com> John, I've used the microwave Bodian stain kit from American Master Tech Item# KTBOD and it worked very well. Have a great weekend! Bea Beatrice DeBrosse-Serra Pathology Scientist Pfizer Global Research & Development CB4, 2150 10646 Science Center Drive San Diego, CA 92121 Phone# 858-622-5986 Fax# 858-678-8290 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Masterson_John Sent: Friday, May 02, 2008 9:31 AM To: Orr, Rebecca; histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) -----Original Message----- Hi Histonetter's, Thanks to all who replied to my last question regarding M.O.M. IHC kits. Does anyone have any wisdom regarding the Bodian stain. I've purchased a kit from a well known vendor but after 4 or 5 attempts I still can't get the section to turn dark tan to brown as described for the protargol step. I've ordered a bottle of pre mixed 1% protargol from another vendor and it doesn't work any better. Any advice? Thanks, John _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ruebenjcarter <@t> gmail.com Fri May 2 13:27:31 2008 From: ruebenjcarter <@t> gmail.com (R C) Date: Fri May 2 13:27:34 2008 Subject: [Histonet] Histology in San Diego Message-ID: <2a926e3f0805021127k8fa51an54e8e001dbefd54d@mail.gmail.com> Cheers. I'm a histo tech relocating to San Diego and am looking for a full time job. I've applied almost everywhere but to no avail. Please let me know of any kown opportunities. Thanks Ruben Carter HT ASCP From jcastell <@t> cbgbiotech.com Fri May 2 13:53:18 2008 From: jcastell <@t> cbgbiotech.com (Julie Castell) Date: Fri May 2 13:53:21 2008 Subject: [Histonet] Benzene, Toulene & Xylene research Message-ID: <002f01c8ac85$c9934980$5cb9dc80$@com> I am working on a research project and am reaching out to my fellow "histoneters" for help. Does anyone know why the histology process changed from using benzene to toluene and now xylene? How did it transition? Kiki Jude Leeman Laboratories Findlay, Oh From PMonfils <@t> Lifespan.org Fri May 2 14:02:40 2008 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri May 2 14:02:46 2008 Subject: [Histonet] Benzene, Toulene & Xylene research In-Reply-To: <002f01c8ac85$c9934980$5cb9dc80$@com> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273D73@LSRIEXCH1.lsmaster.lifespan.org> The change from benzene, even though it did a superb job of clearing tissue, was due to the discovery that benzene is carcinogenic. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Julie Castell > Sent: Friday, May 2, 2008 2:53 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Benzene, Toulene & Xylene research > > The change from benzene, even though it did a superb job of clearing tissue, was due to the discovery that benzene is carcinogenic. > > > > I am working on a research project and am reaching out to my fellow > "histoneters" for help. Does anyone know why the histology process changed > from using benzene to toluene and now xylene? How did it transition? > > > > Kiki Jude > > Leeman Laboratories > > Findlay, Oh > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From jtaylor <@t> meriter.com Fri May 2 14:15:02 2008 From: jtaylor <@t> meriter.com (Taylor, Jean) Date: Fri May 2 14:15:34 2008 Subject: [Histonet] PT module - antigen retrieval Message-ID: <328CBAE62F31C642B422970E879DFADC03A3694F@pcwex01> Hi everyone, I'm testing Lab Vision's PT Module and would like to get feedback from people currently using this system. I believe it's also the same module that Dako sells. One concern I have is with the slides drying out before I have the chance to rinse them. I was always told to never remove slides from hot buffer solution for this reason. Any other pros or cons would be helpful. Thanks! Jean Taylor, HT(ASCP)QIHC Meriter Labs Madison, WI 53715 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, May 02, 2008 12:08 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 54, Issue 2 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: p16ink4 (anh2006@med.cornell.edu) 2. AW: [Histonet] p16ink4 - thanks (Gudrun Lang) 3. Mouse anti-C5b-9 (Angela Bitting) 4. Pneumocystis controls (Jennifer Johnson) 5. Tea Bags (Cindy DuBois) 6. IF muscle cytoskeletal proteins - interior of myocytes doesn't stain (Merced Leiker) 7. Paraffin types (Joyce Cline) 8. (no subject) (Lacy, H Marie) 9. Re: Paraffin types (Gayle Callis) 10. Re: Paraffin types (Anne van Binsbergen) 11. Re: Paraffin types (Piero Nelva) 12. Ventanna Giemsa (Teri.Hallada@midmichigan.org) 13. RE: Ventanna Giemsa (Mike Pence) 14. RE: Paraffin types (Weber, Susan (VHACLE)) 15. DRS 60 jackpot! (Tom McNemar) 16. RE:Pneumocystis controls (Schaundra Walton) 17. IL-1 IL-6 Tnf alpha help (Orr, Rebecca) 18. (no subject) (Masterson_John) ---------------------------------------------------------------------- Message: 1 Date: Thu, 1 May 2008 17:25:16 +0000 From: anh2006@med.cornell.edu Subject: Re: [Histonet] p16ink4 To: "Dana Settembre" , histonet@lists.utsouthwestern.edu Message-ID: <48175415-1209662855-cardhu_decombobulator_blackberry.rim.net-712165279-@bxe115.bisx.prod.on.blackberry> Content-Type: text/plain Cell Signaling and Santa Cruz also sells p16 but I have no idea if it works for IHC. -----Original Message----- From: Dana Settembre Date: Thu, 01 May 2008 12:58:44 To:Gudrun Lang , histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] p16ink4 Gudrun, I believe you are correct mtmlab has a patent and is supposed to be the only people that sell it. Cell Marque was given permission to sell what they had by a certain time, I think. I too only need the antibody and not the whole kit but they have you buy their whole kit and it is expensive. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Gudrun Lang 05/01/08 12:48 PM >>> Hi listmembers, I am trying to find a vendor for p16ink antibody for cervical cancer diagnostic. Is it possible, that p16 is only selled by one firm called mtmlab now? It looks like they have the patent on the antibody. Or is there any other resource? I need "only" the antibody concentrate and not a whole kit to run it on the Ventana benchmark. I found a p16 on the Ventana website, but cannot see, if it is for the same purpose. Any help is appreciated. Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Thu, 1 May 2008 19:37:38 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] p16ink4 - thanks To: Message-ID: <002101c8abb2$0d23d680$eeeea8c0@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" Thank you all for your answers. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von anh2006@med.cornell.edu Gesendet: Donnerstag, 01. Mai 2008 19:25 An: Dana Settembre; histonet@lists.utsouthwestern.edu Betreff: Re: [Histonet] p16ink4 Cell Signaling and Santa Cruz also sells p16 but I have no idea if it works for IHC. -----Original Message----- From: Dana Settembre Date: Thu, 01 May 2008 12:58:44 To:Gudrun Lang , histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] p16ink4 Gudrun, I believe you are correct mtmlab has a patent and is supposed to be the only people that sell it. Cell Marque was given permission to sell what they had by a certain time, I think. I too only need the antibody and not the whole kit but they have you buy their whole kit and it is expensive. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Gudrun Lang 05/01/08 12:48 PM >>> Hi listmembers, I am trying to find a vendor for p16ink antibody for cervical cancer diagnostic. Is it possible, that p16 is only selled by one firm called mtmlab now? It looks like they have the patent on the antibody. Or is there any other resource? I need "only" the antibody concentrate and not a whole kit to run it on the Ventana benchmark. I found a p16 on the Ventana website, but cannot see, if it is for the same purpose. Any help is appreciated. Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Thu, 01 May 2008 14:09:52 -0400 From: "Angela Bitting" Subject: [Histonet] Mouse anti-C5b-9 To: Message-ID: <4819CF30.2B7F.00C9.0@geisinger.edu> Content-Type: text/plain; charset="us-ascii" Is anyone using this antibody for distinction of lupus vs dermatomyositis, and would like to share their automated protocol? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD ------------------------------ Message: 4 Date: Thu, 1 May 2008 14:28:53 -0400 From: Jennifer Johnson Subject: [Histonet] Pneumocystis controls To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" I use Histology Control Systems, Inc for Pneumocystis controls. 1-800-253-2768 or www.HistologyControlSystems.com Also, thanks to everyone who gave me advice on the Bielschowsky stain. One drop of concentrated Hydrochloric acid did NOT yield a positive result. You really do need the ONE drop of concentrated Nitric Acid for the stain to work properly. Jennifer Johnson, HTL (ASCP) _________________________________________________________________ Make i'm yours.? Create a custom banner to support your cause. http://im.live.com/Messenger/IM/Contribute/Default.aspx?source=TXT_TAGHM_MSN_Make_IM_Yours ------------------------------ Message: 5 Date: Thu, 1 May 2008 11:45:17 -0700 From: "Cindy DuBois" Subject: [Histonet] Tea Bags To: histonet@lists.utsouthwestern.edu Message-ID: <5d9104a30805011145l169a3c8m4c8f539c283ce7b2@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 We bought the tea bags and so far have liked them better than the nylon bags for biopsies such as colon, esophagus & gastric. How are they for ECCs and EMB's? My concern is the ECC's are usually very scant and I am afraid what little there is may be absorbed by the paper in the tea bag. What has been your experience? In your experience are they okay to use for scant specimens? Cindy ------------------------------ Message: 6 Date: Thu, 01 May 2008 16:55:58 -0400 From: Merced Leiker Subject: [Histonet] IF muscle cytoskeletal proteins - interior of myocytes doesn't stain To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii; format=flowed Hi, I'm trying to stain hamster skeletal and cardiac muscle for Troponin I, Troponin T, and Myosin Heavy Chain (separately). When I do Z stack imaging at 0.5um steps, only the outermost myofibrils (near the sarcolemma) of most of the myocytes are stained; the interior (particularly of te larger myocytes) is unstained, looking like a gaping black hole. The tissues were snap frozen, cut 8um thick, fixed on the slide in 4%PFA, and permeabilized in 1% Triton. All antibody incubations were done at either 1 hr RT or overnight 4oC. The microscope used was Zeiss Axioimager (epifluorescent). Can you tell me if this is expected staining for these material and conditions, or if something is wrong? Thank you. Merced M Leiker Research Technician II 354 BRB (Lee Lab) / 140 Farber Hall (mail) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 ------------------------------ Message: 7 Date: Thu, 1 May 2008 17:06:57 -0400 From: "Joyce Cline" Subject: [Histonet] Paraffin types To: Message-ID: <000e01c8abcf$4a91d3b0$1d2a14ac@wchsys.org> Content-Type: text/plain; charset="us-ascii" What type of paraffin is everyone using? Separate paraffin for processing and embedding, or the same for processing and embedding? Paraplast, Surgipath, Shandon ???? ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ------------------------------ Message: 8 Date: Thu, 1 May 2008 17:25:17 -0500 From: "Lacy, H Marie" Subject: [Histonet] (no subject) To: histonet@lists.utsouthwestern.edu Message-ID: <8520B38B62EC9B43991358FB6732346C408778@MAIL4.ad.uams.edu> Content-Type: text/plain; charset=us-ascii Good day everyone, Does anyone have a protocol for the naphthol AS-D chloroacetate esterase stain for formalin fixed, paraffin-embedded animal tissue? I am trying to use this stain to identify and count PMNs in sections of guinea pig and mouse tissue. I have tried both Sigma kits for this stain (#90 and #91). They work well on human tissue but not on guinea pig or mouse (even after varying the pH and time). Your help will be greatly appreciated. Marie Lacy Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 9 Date: Thu, 1 May 2008 18:13:23 -0600 From: "Gayle Callis" Subject: Re: [Histonet] Paraffin types To: "Joyce Cline" , Message-ID: <001201c8abe9$561a8f50$6501a8c0@DHXTS541> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Same paraffin for infiltration and embedding. Our choice is especially good for bone because it is a harder paraffin and works well for soft tissues even very thin sections i.g. 1 to 2 um. Since we do a great deal of bone work, this has been a good choice. Tissue Prep 2 from ThermoFisher Scientific. We have used the Surgipath combination infiltration media followed by their embedding media with great success. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Joyce Cline" To: Sent: Thursday, May 01, 2008 3:06 PM Subject: [Histonet] Paraffin types > What type of paraffin is everyone using? Separate paraffin for > processing and embedding, or the same for processing and embedding? > > Paraplast, Surgipath, Shandon ???? > > ------------------------------ Message: 10 Date: Fri, 2 May 2008 10:59:19 +0400 From: "Anne van Binsbergen" Subject: Re: [Histonet] Paraffin types To: "Gayle Callis" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 one size fits all - tissue-tek works for me - been using it for over 10 years now - in all the labs i have managed Annie 2008/5/2 Gayle Callis : > Same paraffin for infiltration and embedding. Our choice is especially > good for bone because it is a harder paraffin and works well for soft > tissues even very thin sections i.g. 1 to 2 um. Since we do a great deal of > bone work, this has been a good choice. Tissue Prep 2 from ThermoFisher > Scientific. > > We have used the Surgipath combination infiltration media followed by > their embedding media with great success. > > Gayle M. Callis > HTL/HT/MT(ASCP) > Bozeman MT 59715 > > > ----- Original Message ----- From: "Joyce Cline" > To: > Sent: Thursday, May 01, 2008 3:06 PM > Subject: [Histonet] Paraffin types > > > What type of paraffin is everyone using? Separate paraffin for > > processing and embedding, or the same for processing and embedding? > > > > Paraplast, Surgipath, Shandon ???? > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne (van Binsbergen) Hope Abu Dhabi UAE ------------------------------ Message: 11 Date: Fri, 2 May 2008 21:12:27 +1000 From: "Piero Nelva" Subject: Re: [Histonet] Paraffin types Cc: Message-ID: <004e01c8ac45$67c761e0$5c72be7c@pentium4> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original I've only ever used the same wax for both processes. Paraplast is the current (>10 years) favorite. Piero ----- Original Message ----- From: "Anne van Binsbergen" To: "Gayle Callis" Cc: Sent: Friday, May 02, 2008 4:59 PM Subject: Re: [Histonet] Paraffin types > one size fits all - tissue-tek works for me - been using it for over 10 > years now - in all the labs i have managed > Annie > > 2008/5/2 Gayle Callis : > >> Same paraffin for infiltration and embedding. Our choice is especially >> good for bone because it is a harder paraffin and works well for soft >> tissues even very thin sections i.g. 1 to 2 um. Since we do a great deal >> of >> bone work, this has been a good choice. Tissue Prep 2 from >> ThermoFisher >> Scientific. >> >> We have used the Surgipath combination infiltration media followed by >> their embedding media with great success. >> >> Gayle M. Callis >> HTL/HT/MT(ASCP) >> Bozeman MT 59715 >> >> >> ----- Original Message ----- From: "Joyce Cline" >> To: >> Sent: Thursday, May 01, 2008 3:06 PM >> Subject: [Histonet] Paraffin types >> >> >> What type of paraffin is everyone using? Separate paraffin for >> > processing and embedding, or the same for processing and embedding? >> > >> > Paraplast, Surgipath, Shandon ???? >> > >> > >> > >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > Anne (van Binsbergen) Hope > Abu Dhabi > UAE > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG. > Version: 7.5.524 / Virus Database: 269.23.7/1410 - Release Date: 5/1/2008 > 5:30 PM > > ------------------------------ Message: 12 Date: Fri, 2 May 2008 08:14:11 -0400 From: Teri.Hallada@midmichigan.org Subject: [Histonet] Ventanna Giemsa To: histonet@pathology.swmed.edu Message-ID: <8839B08E3ED7364E8CBBD53882C984D50994CC67@MAILSRV01.midmichigan.net> Content-Type: text/plain; charset=us-ascii We are having trouble with our Ventanna Giemsa stain for bone marrows. The cells either fall off the slide or explode. Anyone out there willing to share there procedure or have any suggestions? Teresa Hallada BS, MT/CT (ASCP) Lead Cytotechnologist MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. ------------------------------ Message: 13 Date: Fri, 2 May 2008 08:18:42 -0500 From: "Mike Pence" Subject: RE: [Histonet] Ventanna Giemsa To: , Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A380D@IS-E2K3.grhs.net> Content-Type: text/plain; charset="us-ascii" I could never get this stain to work for us and we went back to manual staining. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri.Hallada@midmichigan.org Sent: Friday, May 02, 2008 7:14 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Ventanna Giemsa We are having trouble with our Ventanna Giemsa stain for bone marrows. The cells either fall off the slide or explode. Anyone out there willing to share there procedure or have any suggestions? Teresa Hallada BS, MT/CT (ASCP) Lead Cytotechnologist MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Fri, 2 May 2008 09:56:01 -0400 From: "Weber, Susan (VHACLE)" Subject: RE: [Histonet] Paraffin types To: "Joyce Cline" , Message-ID: <16C83872A53F4346AA9C3A18E3A3AAB903F76D92@VHAV10MSGA1.v10.med.va.gov> Content-Type: text/plain; charset="us-ascii" We are using Surgipath Blue Ribbon Tissue Embedding/Infiltration Medium. As the name implies, we use only this one paraffin for both. Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Thursday, May 01, 2008 5:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin types What type of paraffin is everyone using? Separate paraffin for processing and embedding, or the same for processing and embedding? Paraplast, Surgipath, Shandon ???? ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Fri, 2 May 2008 11:31:33 -0400 From: "Tom McNemar" Subject: [Histonet] DRS 60 jackpot! To: Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F568@lmhsmail.lmhealth.org> Content-Type: text/plain; charset="iso-8859-1" Rummaging around and low and behold.... I found 10-12 plastic staining dishes for the Sakura DRS 60! I know that some of these workhorses are still around and that you probably can't get dishes for them anymore, so.... Send me your fedex number and address and I'll ship them out to anyone who wants them. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ------------------------------ Message: 16 Date: Fri, 2 May 2008 08:48:53 -0700 (PDT) From: Schaundra Walton Subject: [Histonet] RE:Pneumocystis controls To: Histonet Message-ID: <661422.27682.qm@web58909.mail.re1.yahoo.com> Content-Type: text/plain; charset="us-ascii" We just recently ordered these controls slides from Mercedes Medical. [1]www.mercedesmedical.com We were having trouble getting slides from our former supplier and Mercedes' prices were pretty comparable. Hope this helps! Schaundra Walton BS, HTL(ASCP) Histology Supervisor Swedish American Hospital Rockford, IL -----Original Message----- Hoping someone knows of a supplier of pneumocystis TISSUE control slides? Thanks Nancy Rutledge _________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. [2]Try it now. References 1. http://www.mercedesmedical.com/ 2. http://us.rd.yahoo.com/evt=51733/*http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ ------------------------------ Message: 17 Date: Fri, 2 May 2008 11:01:28 -0500 From: "Orr, Rebecca" Subject: [Histonet] IL-1 IL-6 Tnf alpha help To: Message-ID: Content-Type: text/plain; charset="us-ascii" I everyone, I have a researcher who is looking for a lab that will run these antibodies on 24hr Urines. I'm not sure how many samples he has, but if anyone has a reference lab they could recommend I'd be obliged. Mayo does some of these but only on blood/plasma. I'm thinking the specimen type will be the issue. Thanks and let's all have a great weekend. Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 ------------------------------ Message: 18 Date: Fri, 2 May 2008 09:30:57 -0700 From: "Masterson_John" Subject: [Histonet] (no subject) To: "Orr, Rebecca" , Message-ID: <0C58C4F16F0B67448318A38041CADE4B01594E0B@IRMAIL133.irvine.allergan.com> Content-Type: text/plain; charset="us-ascii" -----Original Message----- Hi Histonetter's, Thanks to all who replied to my last question regarding M.O.M. IHC kits. Does anyone have any wisdom regarding the Bodian stain. I've purchased a kit from a well known vendor but after 4 or 5 attempts I still can't get the section to turn dark tan to brown as described for the protargol step. I've ordered a bottle of pre mixed 1% protargol from another vendor and it doesn't work any better. Any advice? Thanks, John ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 54, Issue 2 *************************************** From tp2 <@t> medicine.wisc.edu Fri May 2 14:22:53 2008 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Fri May 2 14:29:12 2008 Subject: [Histonet] PT module - antigen retrieval Message-ID: <481B23BD020000DF000097F8@gwmail.medicine.wisc.edu> I've been using it for about a year now. After the PT Module cools back to 75 degrees, I remove the racks and rinse the slides with TBS-T that has been warmed in a 60 degree oven to rinse away any residual paraffin. Then, I flood the slides with buffer to keep them from drying out. Tom >>> "Taylor, Jean" 05/02/08 2:19 PM >>> Hi everyone, I'm testing Lab Vision's PT Module and would like to get feedback from people currently using this system. I believe it's also the same module that Dako sells. One concern I have is with the slides drying out before I have the chance to rinse them. I was always told to never remove slides from hot buffer solution for this reason. Any other pros or cons would be helpful. Thanks! Jean Taylor, HT(ASCP)QIHC Meriter Labs Madison, WI 53715 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, May 02, 2008 12:08 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 54, Issue 2 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: p16ink4 (anh2006@med.cornell.edu) 2. AW: [Histonet] p16ink4 - thanks (Gudrun Lang) 3. Mouse anti-C5b-9 (Angela Bitting) 4. Pneumocystis controls (Jennifer Johnson) 5. Tea Bags (Cindy DuBois) 6. IF muscle cytoskeletal proteins - interior of myocytes doesn't stain (Merced Leiker) 7. Paraffin types (Joyce Cline) 8. (no subject) (Lacy, H Marie) 9. Re: Paraffin types (Gayle Callis) 10. Re: Paraffin types (Anne van Binsbergen) 11. Re: Paraffin types (Piero Nelva) 12. Ventanna Giemsa (Teri.Hallada@midmichigan.org) 13. RE: Ventanna Giemsa (Mike Pence) 14. RE: Paraffin types (Weber, Susan (VHACLE)) 15. DRS 60 jackpot! (Tom McNemar) 16. RE:Pneumocystis controls (Schaundra Walton) 17. IL-1 IL-6 Tnf alpha help (Orr, Rebecca) 18. (no subject) (Masterson_John) ---------------------------------------------------------------------- Message: 1 Date: Thu, 1 May 2008 17:25:16 +0000 From: anh2006@med.cornell.edu Subject: Re: [Histonet] p16ink4 To: "Dana Settembre" , histonet@lists.utsouthwestern.edu Message-ID: <48175415-1209662855-cardhu_decombobulator_blackberry.rim.net-712165279-@bxe115.bisx.prod.on.blackberry> Content-Type: text/plain Cell Signaling and Santa Cruz also sells p16 but I have no idea if it works for IHC. -----Original Message----- From: Dana Settembre Date: Thu, 01 May 2008 12:58:44 To:Gudrun Lang , histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] p16ink4 Gudrun, I believe you are correct mtmlab has a patent and is supposed to be the only people that sell it. Cell Marque was given permission to sell what they had by a certain time, I think. I too only need the antibody and not the whole kit but they have you buy their whole kit and it is expensive. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Gudrun Lang 05/01/08 12:48 PM >>> Hi listmembers, I am trying to find a vendor for p16ink antibody for cervical cancer diagnostic. Is it possible, that p16 is only selled by one firm called mtmlab now? It looks like they have the patent on the antibody. Or is there any other resource? I need "only" the antibody concentrate and not a whole kit to run it on the Ventana benchmark. I found a p16 on the VenAny help is appreciated. Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Thu, 1 May 2008 19:37:38 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] p16ink4 - thanks To: Message-ID: <002101c8abb2$0d23d680$eeeea8c0@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" Thank you all for your answers. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von anh2006@med.cornell.edu Gesendet: Donnerstag, 01. Mai 2008 19:25 An: Dana Settembre; histonet@lists.utsouthwestern.edu Betreff: Re: [Histonet] p16ink4 Cell Signaling and Santa Cruz also sells p16 but I have no idea if it works for IHC. -----Original Message----- From: Dana Settembre Date: Thu, 01 May 2008 12:58:44 To:Gudrun Lang , histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] p16ink4 Gudrun, I believe you are correct mtmlab has a patent and is supposed to be the only people that sell it. Cell Marque was given permission to sell what they had by a certain time, I think. I too only need the antibody and not the whole kit but they have you buy their whole kit and it is expensive. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Gudrun Lang 05/01/08 12:48 PM >>> Hi listmembers, I am trying to find a vendor for p16ink antibody for cervical cancer diagnostic. Is it possible, that p16 is only selled by one firm called mtmlab now? It looks like they have the patent on the antibody. Or is there any other resource? I need "only" the antibody concentrate and not a whole kit to run it on the Ventana benchmark. I found a p16 on the Ventana website, but cannot see, if it is for the same purpose. Any help is appreciated. Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Thu, 01 May 2008 14:09:52 -0400 From: "Angela Bitting" Subject: [Histonet] Mouse anti-C5b-9 To: Message-ID: <4819CF30.2B7F.00C9.0@geisinger.edu> Content-Type: text/plain; charset="us-ascii" Is anyone using this antibody for distinction of lupus vs dermatomyositis, and would like to share their automated protocol? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD ------------------------------ Message: 4 Date: Thu, 1 May 2008 14:28:53 -0400 From: Jennifer Johnson Content-Type: text/plain; charset="iso-8859-1" I use Histology Control Systems, Inc for Pneumocystis controls. 1-800-253-2768 or www.HistologyControlSystems.com Also, thanks to everyone who gave me advice on the Bielschowsky stain. One drop of concentrated Hydrochloric acid did NOT yield a positive result. You really do need the ONE drop of concentrated Nitric Acid for the stain to work properly. Jennifer Johnson, HTL (ASCP) _________________________________________________________________ Make i'm yours. Create a custom banner to support your cause. http://im.live.com/Messenger/IM/Contribute/Default.aspx?source=TXT_TAGHM_MSN_Make_IM_Yours ------------------------------ Message: 5 Date: Thu, 1 May 2008 11:45:17 -0700 From: "Cindy DuBois" Subject: [Histonet] Tea Bags To: histonet@lists.utsouthwestern.edu Message-ID: <5d9104a30805011145l169a3c8m4c8f539c283ce7b2@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 We bought the tea bags and so far have liked them better than the nylon bags for biopsies such as colon, esophagus & gastric. How are they for ECCs and EMB's? My concern is the ECC's are usually very scant and I am afraid what little there is may be absorbed by the paper in the tea bag. What has been your experience? In your experience are they okay to use for scant specimens? Cindy ------------------------------ Message: 6 Date: Thu, 01 May 2008 16:55:58 -0400 From: Merced Leiker Subject: [Histonet] IF muscle cytoskeletal proteins - interior of myocytes doesn't stain To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii; format=flowed Hi, I'm trying to stain hamster skeletal and cardiac muscle for Troponin I, Troponin T, and Myosin Heavy Chain (separately). When I do Z stack imaging at 0.5um steps, only the outermost myofibrils (near the sarcolemma) of most of the myocytes are stained; the interior (particularly of te larger myocytes) is unstained, looking like a gaping black hole. The tissues were snap frozen, cut 8um thick, fixed on the slide in 4%PFA, and permeabilized in 1% Triton. All antibody incubations were done at either 1 hr RT or overnight 4oC. The microscope used was Zeiss Axioimager (epifluorescent). Can you tell me if this is expected staining for these material and conditions, or if something is wrong? Thank you. Merced M Leiker Research Technician II 354 BRB (Lee Lab) / 140 Farber Hall (mail) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 ------------------------------ Message: 7 Date: Thu, 1 May 2008 17:06:57 -0400 From: "Joyce Cline" Subject: [Histonet] Paraffin types To: Message-ID: <000e01c8abcf$4a91d3b0$1d2a14ac@wchsys.org> Content-Type: text/plain; charset="us-ascii" What type of paraffin is everyone using? Separate paraffin for processing and embedding, or the same for processing and embedding? Paraplast, Surgipath, Shandon ???? ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ------------------------------ Message: 8 Date: Thu, 1 May 2008 17:25:17 -0500 From: "Lacy, H Marie" Subject: [Histonet] (no subject) To: histonet@lists.utsouthwestern.edu Message-ID: <8520B38B62EC9B43991358FB6732346C408778@MAIL4.ad.uams.edu> Content-Type: text/plain; charset=us-ascii Good day everyone, Does anyone have a protocol for the naphthol to use this stain to identify and count PMNs in sections of guinea pig and mouse tissue. I have tried both Sigma kits for this stain (#90 and #91). They work well on human tissue but not on guinea pig or mouse (even after varying the pH and time). Your help will be greatly appreciated. Marie Lacy Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 9 Date: Thu, 1 May 2008 18:13:23 -0600 From: "Gayle Callis" Subject: Re: [Histonet] Paraffin types To: "Joyce Cline" , Message-ID: <001201c8abe9$561a8f50$6501a8c0@DHXTS541> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Same paraffin for infiltration and embedding. Our choice is especially good for bone because it is a harder paraffin and works well for soft tissues even very thin sections i.g. 1 to 2 um. Since we do a great deal of bone work, this has been a good choice. Tissue Prep 2 from ThermoFisher Scientific. We have used the Surgipath combination infiltration media followed by their embedding media with great success. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Joyce Cline" To: Sent: Thursday, May 01, 2008 3:06 PM Subject: [Histonet] Paraffin types > What type of paraffin is everyone using? Separate paraffin for > processing and embedding, or the same for processing and embedding? > > Paraplast, Surgipath, Shandon ???? > > ------------------------------ Message: 10 Date: Fri, 2 May 2008 10:59:19 +0400 From: "Anne van Binsbergen" Subject: Re: [Histonet] Paraffin types To: "Gayle Callis" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 one size fits all - tissue-tek works for me - been using it for over 10 years now - in all the labs i have managed Annie 2008/5/2 Gayle Callis : > Same paraffin for infiltration and embedding. Our choice is especially > good for bone because it is a harder paraffin and works well for soft > tissues even very thin sections i.g. 1 to 2 um. Since we do a great deal of > bone work, this has been a good choice. Tissue Prep 2 from ThermoFisher > Scientific. > > We have used the Surgipath combination infiltration media followed by > their embedding media with great success. > > Gayle M. Callis > HTL/HT/MT(ASCP) > Bozeman MT 59715 > > > ----- Original Message ----- From: "Joyce Cline" > To: > Sent: Thursday, May 01, 2008 3:06 PM > Subject: [Histonet] Paraffin types > > > What type of paraffin is everyone using? Separate paraffin for > > processing and embedding, or the same for processing and embedding? > > > > Paraplast, Surgipath, Shandon ???? > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne (van Binsbergen) Hope Abu Dhabi UAE ------------------------------ Message: 11 Date: Fri, 2 May 2008 21:12:27 +1000 From: "Piero Nelva" Subject: Re: [Histonet] Paraffin types Cc: Message-ID: <004e01c8ac45$67c761e0$5c72be7c@pentium4> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original I've only ever used the samFrom: "Anne van Binsbergen" To: "Gayle Callis" Cc: Sent: Friday, May 02, 2008 4:59 PM Subject: Re: [Histonet] Paraffin types > one size fits all - tissue-tek works for me - been using it for over 10 > years now - in all the labs i have managed > Annie > > 2008/5/2 Gayle Callis : > >> Same paraffin for infiltration and embedding. Our choice is especially >> good for bone because it is a harder paraffin and works well for soft >> tissues even very thin sections i.g. 1 to 2 um. Since we do a great deal >> of >> bone work, this has been a good choice. Tissue Prep 2 from >> ThermoFisher >> Scientific. >> >> We have used the Surgipath combination infiltration media followed by >> their embedding media with great success. >> >> Gayle M. Callis >> HTL/HT/MT(ASCP) >> Bozeman MT 59715 >> >> >> ----- Original Message ----- From: "Joyce Cline" >> To: >> Sent: Thursday, May 01, 2008 3:06 PM >> Subject: [Histonet] Paraffin types >> >> >> What type of paraffin is everyone using? Separate paraffin for >> > processing and embedding, or the same for processing and embedding? >> > >> > Paraplast, Surgipath, Shandon ???? >> > >> > >> > >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > Anne (van Binsbergen) Hope > Abu Dhabi > UAE > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG. > Version: 7.5.524 / Virus Database: 269.23.7/1410 - Release Date: 5/1/2008 > 5:30 PM > > ------------------------------ Message: 12 Date: Fri, 2 May 2008 08:14:11 -0400 From: Teri.Hallada@midmichigan.org Subject: [Histonet] Ventanna Giemsa To: histonet@pathology.swmed.edu Message-ID: <8839B08E3ED7364E8CBBD53882C984D50994CC67@MAILSRV01.midmichigan.net> Content-Type: text/plain; charset=us-ascii We are having trouble with our Ventanna Giemsa stain for bone marrows. The cells either fall off the slide or explode. Anyone out there willing to share there procedure or have any suggestions? Teresa Hallada BS, MT/CT (ASCP) Lead Cytotechnologist MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. ------------------------------ Message: 13 Date: Fri, 2 May 2008 08:18:42 -0500 From: "Mike Pence" Subject: RE: [Histonet] Ventanna Giemsa To: , Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A380D@IS-E2K3.grhs.net> Content-Type: text/plain; charset="us-ascii" I could never get this stain to work for us and we went back to manual staining. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri.Hallada@midmichigan.org Sent: Friday, May 02, 2008 7:14 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Ventanna Giemsa We The cells either fall off the slide or explode. Anyone out there willing to share there procedure or have any suggestions? Teresa Hallada BS, MT/CT (ASCP) Lead Cytotechnologist MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Fri, 2 May 2008 09:56:01 -0400 From: "Weber, Susan (VHACLE)" Subject: RE: [Histonet] Paraffin types To: "Joyce Cline" , Message-ID: <16C83872A53F4346AA9C3A18E3A3AAB903F76D92@VHAV10MSGA1.v10.med.va.gov> Content-Type: text/plain; charset="us-ascii" We are using Surgipath Blue Ribbon Tissue Embedding/Infiltration Medium. As the name implies, we use only this one paraffin for both. Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Thursday, May 01, 2008 5:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin types What type of paraffin is everyone using? Separate paraffin for processing and embedding, or the same for processing and embedding? Paraplast, Surgipath, Shandon ???? ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Fri, 2 May 2008 11:31:33 -0400 From: "Tom McNemar" Subject: [Histonet] DRS 60 jackpot! To: Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F568@lmhsmail.lmhealth.org> Content-Type: text/plain; charset="iso-8859-1" Rummaging around and low and behold.... I found 10-12 plastic staining dishes for the Sakura DRS 60! I know that some of these workhorses are still around and that you probably can't get dishes for them anymore, so.... Send me your fedex number and address and I'll ship them out to anyone who wants them. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ------------------------------ Message: 16 Date: Fri, 2 May 2008 08:48:53 -0700 (PDT) From: Schaundra Walton Subject: [Histonet] RE:Pneumocystis controls To: Histonet Message-ID: <661422.27682.qm@web58909.mail.re1.yahoo.com> Content-Type: text/plain; charset="us-ascii" We just recently ordered these controls slides from Mercedes Medical. [1]www.mercedesmedical and Mercedes' prices were pretty comparable. Hope this helps! Schaundra Walton BS, HTL(ASCP) Histology Supervisor Swedish American Hospital Rockford, IL -----Original Message----- Hoping someone knows of a supplier of pneumocystis TISSUE control slides? Thanks Nancy Rutledge _________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. [2]Try it now. References 1. http://www.mercedesmedical.com/ 2. http://us.rd.yahoo.com/evt=51733/*http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ ------------------------------ Message: 17 Date: Fri, 2 May 2008 11:01:28 -0500 From: "Orr, Rebecca" Subject: [Histonet] IL-1 IL-6 Tnf alpha help To: Message-ID: Content-Type: text/plain; charset="us-ascii" I everyone, I have a researcher who is looking for a lab that will run these antibodies on 24hr Urines. I'm not sure how many samples he has, but if anyone has a reference lab they could recommend I'd be obliged. Mayo does some of these but only on blood/plasma. I'm thinking the specimen type will be the issue. Thanks and let's all have a great weekend. Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 ------------------------------ Message: 18 Date: Fri, 2 May 2008 09:30:57 -0700 From: "Masterson_John" Subject: [Histonet] (no subject) To: "Orr, Rebecca" , Message-ID: <0C58C4F16F0B67448318A38041CADE4B01594E0B@IRMAIL133.irvine.allergan.com> Content-Type: text/plain; charset="us-ascii" -----Original Message----- Hi Histonetter's, Thanks to all who replied to my last question regarding M.O.M. IHC kits. Does anyone have any wisdom regarding the Bodian stain. I've purchased a kit from a well known vendor but after 4 or 5 attempts I still can't get the section to turn dark tan to brown as described for the protargol step. I've ordered a bottle of pre mixed 1% protargol from another vendor and it doesn't work any better. Any advice? Thanks, John ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 54, Issue 2 *************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kathy.Abels <@t> sial.com Fri May 2 16:00:46 2008 From: Kathy.Abels <@t> sial.com (Kathy Abels) Date: Fri May 2 16:01:16 2008 Subject: [Histonet] Kathy Abels/ops/diag/sial is out of the office. Message-ID: I will be out of the office starting 05/01/2008 and will not return until 05/05/2008. I will respond to your message when I return. For urgent matters, Please contact Roy Street.. This message and any files transmitted with it are the property of Sigma-Aldrich Corporation, are confidential, and are intended solely for the use of the person or entity to whom this e-mail is addressed. If you are not one of the named recipient(s) or otherwise have reason to believe that you have received this message in error, please contact the sender and delete this message immediately from your computer. Any other use, retention, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. From bmbow <@t> seagen.com Fri May 2 19:10:24 2008 From: bmbow <@t> seagen.com (Brianna Mbow) Date: Fri May 2 19:10:30 2008 Subject: [Histonet] NaBH4 in autostainer Message-ID: <9916263273C7B945BE6FF9ACBDD6C50A035919D1@SGEXCH.corp.seagen.com> Hi all, I would like to try using sodium borohydride to aid in reducing autofluorescence from formalin fixation. I'm trying to adapt the protocol to an autostainer. Has anyone tried this? I'm wondering if I should worry about how the solution will react with the rest of the waste products once it is discarded. Does anyone have any thoughts? Thanks, Brianna Mbow Seattle Genetics Bothell, WA From MElliott <@t> mrl.ubc.ca Fri May 2 22:07:33 2008 From: MElliott <@t> mrl.ubc.ca (Mark Elliott) Date: Fri May 2 22:08:14 2008 Subject: [Histonet] Gallon size Shandon Cryomatrix Message-ID: <481B7484.11C6.00D6.0@mrl.ubc.ca> I have just found out that Shandon is no longer selling the 1 gallon size of Cryomatix, only the smaller bottles. The problem is that for processing human lungs I can go through 500-100 mls or more at one time, so the large bottles were more cost effective. Price I am being quoted now will over double my cost. Does anyone know of a supplier who will sell it in gallon size bottles of similar product? Thanks and have a great weekend Mark ***CONFIDENTIALITY NOTICE*** This electronic message is intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From mickie25 <@t> netzero.net Sat May 3 08:34:49 2008 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Sat May 3 08:34:41 2008 Subject: [Histonet] Question about Energy Beam Microwave Processor H2800 In-Reply-To: <328CBAE62F31C642B422970E879DFADC03A3694F@pcwex01> References: <328CBAE62F31C642B422970E879DFADC03A3694F@pcwex01> Message-ID: Dear Histonetters, I have been asked to evaluate the usefulness of an Energy Beam Microwave Processor, Model # H2800. This unit is a 220V model which does not include a carousel. It is about 10 years old. The intended use is to process dermatological biopsies, shaves and excisions. Is this unit useful for this purpose and is the unit still appropriate for this use or should the derm laboratory abandon this in favor of conventional vacuum infiltration processor of the VIP variety? Any help, direction or input would be greatly appreciated! Thank you. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean Sent: Friday, May 02, 2008 12:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PT module - antigen retrieval Hi everyone, I'm testing Lab Vision's PT Module and would like to get feedback from people currently using this system. I believe it's also the same module that Dako sells. One concern I have is with the slides drying out before I have the chance to rinse them. I was always told to never remove slides from hot buffer solution for this reason. Any other pros or cons would be helpful. Thanks! Jean Taylor, HT(ASCP)QIHC Meriter Labs Madison, WI 53715 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, May 02, 2008 12:08 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 54, Issue 2 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: p16ink4 (anh2006@med.cornell.edu) 2. AW: [Histonet] p16ink4 - thanks (Gudrun Lang) 3. Mouse anti-C5b-9 (Angela Bitting) 4. Pneumocystis controls (Jennifer Johnson) 5. Tea Bags (Cindy DuBois) 6. IF muscle cytoskeletal proteins - interior of myocytes doesn't stain (Merced Leiker) 7. Paraffin types (Joyce Cline) 8. (no subject) (Lacy, H Marie) 9. Re: Paraffin types (Gayle Callis) 10. Re: Paraffin types (Anne van Binsbergen) 11. Re: Paraffin types (Piero Nelva) 12. Ventanna Giemsa (Teri.Hallada@midmichigan.org) 13. RE: Ventanna Giemsa (Mike Pence) 14. RE: Paraffin types (Weber, Susan (VHACLE)) 15. DRS 60 jackpot! (Tom McNemar) 16. RE:Pneumocystis controls (Schaundra Walton) 17. IL-1 IL-6 Tnf alpha help (Orr, Rebecca) 18. (no subject) (Masterson_John) ---------------------------------------------------------------------- Message: 1 Date: Thu, 1 May 2008 17:25:16 +0000 From: anh2006@med.cornell.edu Subject: Re: [Histonet] p16ink4 To: "Dana Settembre" , histonet@lists.utsouthwestern.edu Message-ID: <48175415-1209662855-cardhu_decombobulator_blackberry.rim.net-712165279-@bxe 115.bisx.prod.on.blackberry> Content-Type: text/plain Cell Signaling and Santa Cruz also sells p16 but I have no idea if it works for IHC. -----Original Message----- From: Dana Settembre Date: Thu, 01 May 2008 12:58:44 To:Gudrun Lang , histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] p16ink4 Gudrun, I believe you are correct mtmlab has a patent and is supposed to be the only people that sell it. Cell Marque was given permission to sell what they had by a certain time, I think. I too only need the antibody and not the whole kit but they have you buy their whole kit and it is expensive. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Gudrun Lang 05/01/08 12:48 PM >>> Hi listmembers, I am trying to find a vendor for p16ink antibody for cervical cancer diagnostic. Is it possible, that p16 is only selled by one firm called mtmlab now? It looks like they have the patent on the antibody. Or is there any other resource? I need "only" the antibody concentrate and not a whole kit to run it on the Ventana benchmark. I found a p16 on the Ventana website, but cannot see, if it is for the same purpose. Any help is appreciated. Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Thu, 1 May 2008 19:37:38 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] p16ink4 - thanks To: Message-ID: <002101c8abb2$0d23d680$eeeea8c0@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" Thank you all for your answers. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von anh2006@med.cornell.edu Gesendet: Donnerstag, 01. Mai 2008 19:25 An: Dana Settembre; histonet@lists.utsouthwestern.edu Betreff: Re: [Histonet] p16ink4 Cell Signaling and Santa Cruz also sells p16 but I have no idea if it works for IHC. -----Original Message----- From: Dana Settembre Date: Thu, 01 May 2008 12:58:44 To:Gudrun Lang , histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] p16ink4 Gudrun, I believe you are correct mtmlab has a patent and is supposed to be the only people that sell it. Cell Marque was given permission to sell what they had by a certain time, I think. I too only need the antibody and not the whole kit but they have you buy their whole kit and it is expensive. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Gudrun Lang 05/01/08 12:48 PM >>> Hi listmembers, I am trying to find a vendor for p16ink antibody for cervical cancer diagnostic. Is it possible, that p16 is only selled by one firm called mtmlab now? It looks like they have the patent on the antibody. Or is there any other resource? I need "only" the antibody concentrate and not a whole kit to run it on the Ventana benchmark. I found a p16 on the Ventana website, but cannot see, if it is for the same purpose. Any help is appreciated. Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Thu, 01 May 2008 14:09:52 -0400 From: "Angela Bitting" Subject: [Histonet] Mouse anti-C5b-9 To: Message-ID: <4819CF30.2B7F.00C9.0@geisinger.edu> Content-Type: text/plain; charset="us-ascii" Is anyone using this antibody for distinction of lupus vs dermatomyositis, and would like to share their automated protocol? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD ------------------------------ Message: 4 Date: Thu, 1 May 2008 14:28:53 -0400 From: Jennifer Johnson Subject: [Histonet] Pneumocystis controls To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" I use Histology Control Systems, Inc for Pneumocystis controls. 1-800-253-2768 or www.HistologyControlSystems.com Also, thanks to everyone who gave me advice on the Bielschowsky stain. One drop of concentrated Hydrochloric acid did NOT yield a positive result. You really do need the ONE drop of concentrated Nitric Acid for the stain to work properly. Jennifer Johnson, HTL (ASCP) _________________________________________________________________ Make i'm yours.? Create a custom banner to support your cause. http://im.live.com/Messenger/IM/Contribute/Default.aspx?source=TXT_TAGHM_MSN _Make_IM_Yours ------------------------------ Message: 5 Date: Thu, 1 May 2008 11:45:17 -0700 From: "Cindy DuBois" Subject: [Histonet] Tea Bags To: histonet@lists.utsouthwestern.edu Message-ID: <5d9104a30805011145l169a3c8m4c8f539c283ce7b2@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 We bought the tea bags and so far have liked them better than the nylon bags for biopsies such as colon, esophagus & gastric. How are they for ECCs and EMB's? My concern is the ECC's are usually very scant and I am afraid what little there is may be absorbed by the paper in the tea bag. What has been your experience? In your experience are they okay to use for scant specimens? Cindy ------------------------------ Message: 6 Date: Thu, 01 May 2008 16:55:58 -0400 From: Merced Leiker Subject: [Histonet] IF muscle cytoskeletal proteins - interior of myocytes doesn't stain To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii; format=flowed Hi, I'm trying to stain hamster skeletal and cardiac muscle for Troponin I, Troponin T, and Myosin Heavy Chain (separately). When I do Z stack imaging at 0.5um steps, only the outermost myofibrils (near the sarcolemma) of most of the myocytes are stained; the interior (particularly of te larger myocytes) is unstained, looking like a gaping black hole. The tissues were snap frozen, cut 8um thick, fixed on the slide in 4%PFA, and permeabilized in 1% Triton. All antibody incubations were done at either 1 hr RT or overnight 4oC. The microscope used was Zeiss Axioimager (epifluorescent). Can you tell me if this is expected staining for these material and conditions, or if something is wrong? Thank you. Merced M Leiker Research Technician II 354 BRB (Lee Lab) / 140 Farber Hall (mail) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 ------------------------------ Message: 7 Date: Thu, 1 May 2008 17:06:57 -0400 From: "Joyce Cline" Subject: [Histonet] Paraffin types To: Message-ID: <000e01c8abcf$4a91d3b0$1d2a14ac@wchsys.org> Content-Type: text/plain; charset="us-ascii" What type of paraffin is everyone using? Separate paraffin for processing and embedding, or the same for processing and embedding? Paraplast, Surgipath, Shandon ???? ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ------------------------------ Message: 8 Date: Thu, 1 May 2008 17:25:17 -0500 From: "Lacy, H Marie" Subject: [Histonet] (no subject) To: histonet@lists.utsouthwestern.edu Message-ID: <8520B38B62EC9B43991358FB6732346C408778@MAIL4.ad.uams.edu> Content-Type: text/plain; charset=us-ascii Good day everyone, Does anyone have a protocol for the naphthol AS-D chloroacetate esterase stain for formalin fixed, paraffin-embedded animal tissue? I am trying to use this stain to identify and count PMNs in sections of guinea pig and mouse tissue. I have tried both Sigma kits for this stain (#90 and #91). They work well on human tissue but not on guinea pig or mouse (even after varying the pH and time). Your help will be greatly appreciated. Marie Lacy Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 9 Date: Thu, 1 May 2008 18:13:23 -0600 From: "Gayle Callis" Subject: Re: [Histonet] Paraffin types To: "Joyce Cline" , Message-ID: <001201c8abe9$561a8f50$6501a8c0@DHXTS541> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Same paraffin for infiltration and embedding. Our choice is especially good for bone because it is a harder paraffin and works well for soft tissues even very thin sections i.g. 1 to 2 um. Since we do a great deal of bone work, this has been a good choice. Tissue Prep 2 from ThermoFisher Scientific. We have used the Surgipath combination infiltration media followed by their embedding media with great success. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Joyce Cline" To: Sent: Thursday, May 01, 2008 3:06 PM Subject: [Histonet] Paraffin types > What type of paraffin is everyone using? Separate paraffin for > processing and embedding, or the same for processing and embedding? > > Paraplast, Surgipath, Shandon ???? > > ------------------------------ Message: 10 Date: Fri, 2 May 2008 10:59:19 +0400 From: "Anne van Binsbergen" Subject: Re: [Histonet] Paraffin types To: "Gayle Callis" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 one size fits all - tissue-tek works for me - been using it for over 10 years now - in all the labs i have managed Annie 2008/5/2 Gayle Callis : > Same paraffin for infiltration and embedding. Our choice is especially > good for bone because it is a harder paraffin and works well for soft > tissues even very thin sections i.g. 1 to 2 um. Since we do a great deal of > bone work, this has been a good choice. Tissue Prep 2 from ThermoFisher > Scientific. > > We have used the Surgipath combination infiltration media followed by > their embedding media with great success. > > Gayle M. Callis > HTL/HT/MT(ASCP) > Bozeman MT 59715 > > > ----- Original Message ----- From: "Joyce Cline" > To: > Sent: Thursday, May 01, 2008 3:06 PM > Subject: [Histonet] Paraffin types > > > What type of paraffin is everyone using? Separate paraffin for > > processing and embedding, or the same for processing and embedding? > > > > Paraplast, Surgipath, Shandon ???? > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne (van Binsbergen) Hope Abu Dhabi UAE ------------------------------ Message: 11 Date: Fri, 2 May 2008 21:12:27 +1000 From: "Piero Nelva" Subject: Re: [Histonet] Paraffin types Cc: Message-ID: <004e01c8ac45$67c761e0$5c72be7c@pentium4> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original I've only ever used the same wax for both processes. Paraplast is the current (>10 years) favorite. Piero ----- Original Message ----- From: "Anne van Binsbergen" To: "Gayle Callis" Cc: Sent: Friday, May 02, 2008 4:59 PM Subject: Re: [Histonet] Paraffin types > one size fits all - tissue-tek works for me - been using it for over 10 > years now - in all the labs i have managed > Annie > > 2008/5/2 Gayle Callis : > >> Same paraffin for infiltration and embedding. Our choice is especially >> good for bone because it is a harder paraffin and works well for soft >> tissues even very thin sections i.g. 1 to 2 um. Since we do a great deal >> of >> bone work, this has been a good choice. Tissue Prep 2 from >> ThermoFisher >> Scientific. >> >> We have used the Surgipath combination infiltration media followed by >> their embedding media with great success. >> >> Gayle M. Callis >> HTL/HT/MT(ASCP) >> Bozeman MT 59715 >> >> >> ----- Original Message ----- From: "Joyce Cline" >> To: >> Sent: Thursday, May 01, 2008 3:06 PM >> Subject: [Histonet] Paraffin types >> >> >> What type of paraffin is everyone using? Separate paraffin for >> > processing and embedding, or the same for processing and embedding? >> > >> > Paraplast, Surgipath, Shandon ???? >> > >> > >> > >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > Anne (van Binsbergen) Hope > Abu Dhabi > UAE > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG. > Version: 7.5.524 / Virus Database: 269.23.7/1410 - Release Date: 5/1/2008 > 5:30 PM > > ------------------------------ Message: 12 Date: Fri, 2 May 2008 08:14:11 -0400 From: Teri.Hallada@midmichigan.org Subject: [Histonet] Ventanna Giemsa To: histonet@pathology.swmed.edu Message-ID: <8839B08E3ED7364E8CBBD53882C984D50994CC67@MAILSRV01.midmichigan.net> Content-Type: text/plain; charset=us-ascii We are having trouble with our Ventanna Giemsa stain for bone marrows. The cells either fall off the slide or explode. Anyone out there willing to share there procedure or have any suggestions? Teresa Hallada BS, MT/CT (ASCP) Lead Cytotechnologist MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. ------------------------------ Message: 13 Date: Fri, 2 May 2008 08:18:42 -0500 From: "Mike Pence" Subject: RE: [Histonet] Ventanna Giemsa To: , Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A380D@IS-E2K3.grhs.net> Content-Type: text/plain; charset="us-ascii" I could never get this stain to work for us and we went back to manual staining. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri.Hallada@midmichigan.org Sent: Friday, May 02, 2008 7:14 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Ventanna Giemsa We are having trouble with our Ventanna Giemsa stain for bone marrows. The cells either fall off the slide or explode. Anyone out there willing to share there procedure or have any suggestions? Teresa Hallada BS, MT/CT (ASCP) Lead Cytotechnologist MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Fri, 2 May 2008 09:56:01 -0400 From: "Weber, Susan (VHACLE)" Subject: RE: [Histonet] Paraffin types To: "Joyce Cline" , Message-ID: <16C83872A53F4346AA9C3A18E3A3AAB903F76D92@VHAV10MSGA1.v10.med.va.gov> Content-Type: text/plain; charset="us-ascii" We are using Surgipath Blue Ribbon Tissue Embedding/Infiltration Medium. As the name implies, we use only this one paraffin for both. Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Thursday, May 01, 2008 5:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin types What type of paraffin is everyone using? Separate paraffin for processing and embedding, or the same for processing and embedding? Paraplast, Surgipath, Shandon ???? ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Fri, 2 May 2008 11:31:33 -0400 From: "Tom McNemar" Subject: [Histonet] DRS 60 jackpot! To: Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F568@lmhsmail.lmhealth.org> Content-Type: text/plain; charset="iso-8859-1" Rummaging around and low and behold.... I found 10-12 plastic staining dishes for the Sakura DRS 60! I know that some of these workhorses are still around and that you probably can't get dishes for them anymore, so.... Send me your fedex number and address and I'll ship them out to anyone who wants them. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ------------------------------ Message: 16 Date: Fri, 2 May 2008 08:48:53 -0700 (PDT) From: Schaundra Walton Subject: [Histonet] RE:Pneumocystis controls To: Histonet Message-ID: <661422.27682.qm@web58909.mail.re1.yahoo.com> Content-Type: text/plain; charset="us-ascii" We just recently ordered these controls slides from Mercedes Medical. [1]www.mercedesmedical.com We were having trouble getting slides from our former supplier and Mercedes' prices were pretty comparable. Hope this helps! Schaundra Walton BS, HTL(ASCP) Histology Supervisor Swedish American Hospital Rockford, IL -----Original Message----- Hoping someone knows of a supplier of pneumocystis TISSUE control slides? Thanks Nancy Rutledge _________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. [2]Try it now. References 1. http://www.mercedesmedical.com/ 2. http://us.rd.yahoo.com/evt=51733/*http://mobile.yahoo.com/;_ylt=Ahu06i62sR8H DtDypao8Wcj9tAcJ ------------------------------ Message: 17 Date: Fri, 2 May 2008 11:01:28 -0500 From: "Orr, Rebecca" Subject: [Histonet] IL-1 IL-6 Tnf alpha help To: Message-ID: Content-Type: text/plain; charset="us-ascii" I everyone, I have a researcher who is looking for a lab that will run these antibodies on 24hr Urines. I'm not sure how many samples he has, but if anyone has a reference lab they could recommend I'd be obliged. Mayo does some of these but only on blood/plasma. I'm thinking the specimen type will be the issue. Thanks and let's all have a great weekend. Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 ------------------------------ Message: 18 Date: Fri, 2 May 2008 09:30:57 -0700 From: "Masterson_John" Subject: [Histonet] (no subject) To: "Orr, Rebecca" , Message-ID: <0C58C4F16F0B67448318A38041CADE4B01594E0B@IRMAIL133.irvine.allergan.com> Content-Type: text/plain; charset="us-ascii" -----Original Message----- Hi Histonetter's, Thanks to all who replied to my last question regarding M.O.M. IHC kits. Does anyone have any wisdom regarding the Bodian stain. I've purchased a kit from a well known vendor but after 4 or 5 attempts I still can't get the section to turn dark tan to brown as described for the protargol step. I've ordered a bottle of pre mixed 1% protargol from another vendor and it doesn't work any better. Any advice? Thanks, John ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 54, Issue 2 *************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maxim_71 <@t> mail.ru Sat May 3 13:44:24 2008 From: Maxim_71 <@t> mail.ru (Maxim_71@mail.ru) Date: Sat May 3 13:45:48 2008 Subject: [Histonet] Re: Histonet Digest, Vol 54, Issue 3 In-Reply-To: <1209822465.1014012607@mx42.mail.ru> References: <1209822465.1014012607@mx42.mail.ru> Message-ID: <1171404026.20080503224424@mail.ru> Julie: Safety here is a major consideration. Click here and read this info: http://www.anatechltdusa.com/Innovators/5_InnXylHaz.html Here is a good explanations why xylene is hazardous. There are more safe reagents than xylene and its substitutes: mineral oil. It possible use as in manual processing as VIP-processors as microwave assisted manual method as in microwave walk-away type processors. I believe, that through some 4-5 years will not remain nor one laboratory, using xylene for processing. Our lab tried chloroform, benzene, toluene, xylene and xylene substitutes for processing. All chemicals are hazardous for personnel. Now we uses mineral oil and processes our all specimens manually with best results and in safety environment. Sincerely, Maxim Peshkov, Russia, Taganrog. ---original message--- > Date: Fri, 2 May 2008 14:53:18 -0400 > From: "Julie Castell" > Subject: [Histonet] Benzene, Toulene & Xylene research > To: > Message-ID: <002f01c8ac85$c9934980$5cb9dc80$@com> > Content-Type: text/plain; charset="us-ascii" > > > > > > I am working on a research project and am reaching > out to my fellow > "histoneters" for help. Does anyone know why the > histology process changed > from using benzene to toluene and now xylene? How > did it transition? > > > > Kiki Jude > > Leeman Laboratories > > Findlay, Oh From mickie25 <@t> netzero.net Sat May 3 18:20:31 2008 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Sat May 3 18:20:22 2008 Subject: [Histonet] Question about Energy Beam Microwave Processor H2800 In-Reply-To: References: <328CBAE62F31C642B422970E879DFADC03A3694F@pcwex01> Message-ID: Hi Histonetters, I just thought I would clarify that my client already owns this microwave processor and will be processing only about 3000 biopsies a year. If you have any experience or advice whether this is suitable for a small lab with a single tech doing Mohs and routine biopsies, I would love to hear from you. Thank you very much. Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mickie Johnson Sent: Saturday, May 03, 2008 6:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question about Energy Beam Microwave Processor H2800 Dear Histonetters, I have been asked to evaluate the usefulness of an Energy Beam Microwave Processor, Model # H2800. This unit is a 220V model which does not include a carousel. It is about 10 years old. The intended use is to process dermatological biopsies, shaves and excisions. Is this unit useful for this purpose and is the unit still appropriate for this use or should the derm laboratory abandon this in favor of conventional vacuum infiltration processor of the VIP variety? Any help, direction or input would be greatly appreciated! Thank you. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean Sent: Friday, May 02, 2008 12:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PT module - antigen retrieval Hi everyone, I'm testing Lab Vision's PT Module and would like to get feedback from people currently using this system. I believe it's also the same module that Dako sells. One concern I have is with the slides drying out before I have the chance to rinse them. I was always told to never remove slides from hot buffer solution for this reason. Any other pros or cons would be helpful. Thanks! Jean Taylor, HT(ASCP)QIHC Meriter Labs Madison, WI 53715 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, May 02, 2008 12:08 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 54, Issue 2 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: p16ink4 (anh2006@med.cornell.edu) 2. AW: [Histonet] p16ink4 - thanks (Gudrun Lang) 3. Mouse anti-C5b-9 (Angela Bitting) 4. Pneumocystis controls (Jennifer Johnson) 5. Tea Bags (Cindy DuBois) 6. IF muscle cytoskeletal proteins - interior of myocytes doesn't stain (Merced Leiker) 7. Paraffin types (Joyce Cline) 8. (no subject) (Lacy, H Marie) 9. Re: Paraffin types (Gayle Callis) 10. Re: Paraffin types (Anne van Binsbergen) 11. Re: Paraffin types (Piero Nelva) 12. Ventanna Giemsa (Teri.Hallada@midmichigan.org) 13. RE: Ventanna Giemsa (Mike Pence) 14. RE: Paraffin types (Weber, Susan (VHACLE)) 15. DRS 60 jackpot! (Tom McNemar) 16. RE:Pneumocystis controls (Schaundra Walton) 17. IL-1 IL-6 Tnf alpha help (Orr, Rebecca) 18. (no subject) (Masterson_John) ---------------------------------------------------------------------- Message: 1 Date: Thu, 1 May 2008 17:25:16 +0000 From: anh2006@med.cornell.edu Subject: Re: [Histonet] p16ink4 To: "Dana Settembre" , histonet@lists.utsouthwestern.edu Message-ID: <48175415-1209662855-cardhu_decombobulator_blackberry.rim.net-712165279-@bxe 115.bisx.prod.on.blackberry> Content-Type: text/plain Cell Signaling and Santa Cruz also sells p16 but I have no idea if it works for IHC. -----Original Message----- From: Dana Settembre Date: Thu, 01 May 2008 12:58:44 To:Gudrun Lang , histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] p16ink4 Gudrun, I believe you are correct mtmlab has a patent and is supposed to be the only people that sell it. Cell Marque was given permission to sell what they had by a certain time, I think. I too only need the antibody and not the whole kit but they have you buy their whole kit and it is expensive. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Gudrun Lang 05/01/08 12:48 PM >>> Hi listmembers, I am trying to find a vendor for p16ink antibody for cervical cancer diagnostic. Is it possible, that p16 is only selled by one firm called mtmlab now? It looks like they have the patent on the antibody. Or is there any other resource? I need "only" the antibody concentrate and not a whole kit to run it on the Ventana benchmark. I found a p16 on the Ventana website, but cannot see, if it is for the same purpose. Any help is appreciated. Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Thu, 1 May 2008 19:37:38 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] p16ink4 - thanks To: Message-ID: <002101c8abb2$0d23d680$eeeea8c0@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" Thank you all for your answers. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von anh2006@med.cornell.edu Gesendet: Donnerstag, 01. Mai 2008 19:25 An: Dana Settembre; histonet@lists.utsouthwestern.edu Betreff: Re: [Histonet] p16ink4 Cell Signaling and Santa Cruz also sells p16 but I have no idea if it works for IHC. -----Original Message----- From: Dana Settembre Date: Thu, 01 May 2008 12:58:44 To:Gudrun Lang , histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] p16ink4 Gudrun, I believe you are correct mtmlab has a patent and is supposed to be the only people that sell it. Cell Marque was given permission to sell what they had by a certain time, I think. I too only need the antibody and not the whole kit but they have you buy their whole kit and it is expensive. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Gudrun Lang 05/01/08 12:48 PM >>> Hi listmembers, I am trying to find a vendor for p16ink antibody for cervical cancer diagnostic. Is it possible, that p16 is only selled by one firm called mtmlab now? It looks like they have the patent on the antibody. Or is there any other resource? I need "only" the antibody concentrate and not a whole kit to run it on the Ventana benchmark. I found a p16 on the Ventana website, but cannot see, if it is for the same purpose. Any help is appreciated. Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Thu, 01 May 2008 14:09:52 -0400 From: "Angela Bitting" Subject: [Histonet] Mouse anti-C5b-9 To: Message-ID: <4819CF30.2B7F.00C9.0@geisinger.edu> Content-Type: text/plain; charset="us-ascii" Is anyone using this antibody for distinction of lupus vs dermatomyositis, and would like to share their automated protocol? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD ------------------------------ Message: 4 Date: Thu, 1 May 2008 14:28:53 -0400 From: Jennifer Johnson Subject: [Histonet] Pneumocystis controls To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" I use Histology Control Systems, Inc for Pneumocystis controls. 1-800-253-2768 or www.HistologyControlSystems.com Also, thanks to everyone who gave me advice on the Bielschowsky stain. One drop of concentrated Hydrochloric acid did NOT yield a positive result. You really do need the ONE drop of concentrated Nitric Acid for the stain to work properly. Jennifer Johnson, HTL (ASCP) _________________________________________________________________ Make i'm yours.? Create a custom banner to support your cause. http://im.live.com/Messenger/IM/Contribute/Default.aspx?source=TXT_TAGHM_MSN _Make_IM_Yours ------------------------------ Message: 5 Date: Thu, 1 May 2008 11:45:17 -0700 From: "Cindy DuBois" Subject: [Histonet] Tea Bags To: histonet@lists.utsouthwestern.edu Message-ID: <5d9104a30805011145l169a3c8m4c8f539c283ce7b2@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 We bought the tea bags and so far have liked them better than the nylon bags for biopsies such as colon, esophagus & gastric. How are they for ECCs and EMB's? My concern is the ECC's are usually very scant and I am afraid what little there is may be absorbed by the paper in the tea bag. What has been your experience? In your experience are they okay to use for scant specimens? Cindy ------------------------------ Message: 6 Date: Thu, 01 May 2008 16:55:58 -0400 From: Merced Leiker Subject: [Histonet] IF muscle cytoskeletal proteins - interior of myocytes doesn't stain To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii; format=flowed Hi, I'm trying to stain hamster skeletal and cardiac muscle for Troponin I, Troponin T, and Myosin Heavy Chain (separately). When I do Z stack imaging at 0.5um steps, only the outermost myofibrils (near the sarcolemma) of most of the myocytes are stained; the interior (particularly of te larger myocytes) is unstained, looking like a gaping black hole. The tissues were snap frozen, cut 8um thick, fixed on the slide in 4%PFA, and permeabilized in 1% Triton. All antibody incubations were done at either 1 hr RT or overnight 4oC. The microscope used was Zeiss Axioimager (epifluorescent). Can you tell me if this is expected staining for these material and conditions, or if something is wrong? Thank you. Merced M Leiker Research Technician II 354 BRB (Lee Lab) / 140 Farber Hall (mail) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 ------------------------------ Message: 7 Date: Thu, 1 May 2008 17:06:57 -0400 From: "Joyce Cline" Subject: [Histonet] Paraffin types To: Message-ID: <000e01c8abcf$4a91d3b0$1d2a14ac@wchsys.org> Content-Type: text/plain; charset="us-ascii" What type of paraffin is everyone using? Separate paraffin for processing and embedding, or the same for processing and embedding? Paraplast, Surgipath, Shandon ???? ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ------------------------------ Message: 8 Date: Thu, 1 May 2008 17:25:17 -0500 From: "Lacy, H Marie" Subject: [Histonet] (no subject) To: histonet@lists.utsouthwestern.edu Message-ID: <8520B38B62EC9B43991358FB6732346C408778@MAIL4.ad.uams.edu> Content-Type: text/plain; charset=us-ascii Good day everyone, Does anyone have a protocol for the naphthol AS-D chloroacetate esterase stain for formalin fixed, paraffin-embedded animal tissue? I am trying to use this stain to identify and count PMNs in sections of guinea pig and mouse tissue. I have tried both Sigma kits for this stain (#90 and #91). They work well on human tissue but not on guinea pig or mouse (even after varying the pH and time). Your help will be greatly appreciated. Marie Lacy Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 9 Date: Thu, 1 May 2008 18:13:23 -0600 From: "Gayle Callis" Subject: Re: [Histonet] Paraffin types To: "Joyce Cline" , Message-ID: <001201c8abe9$561a8f50$6501a8c0@DHXTS541> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Same paraffin for infiltration and embedding. Our choice is especially good for bone because it is a harder paraffin and works well for soft tissues even very thin sections i.g. 1 to 2 um. Since we do a great deal of bone work, this has been a good choice. Tissue Prep 2 from ThermoFisher Scientific. We have used the Surgipath combination infiltration media followed by their embedding media with great success. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Joyce Cline" To: Sent: Thursday, May 01, 2008 3:06 PM Subject: [Histonet] Paraffin types > What type of paraffin is everyone using? Separate paraffin for > processing and embedding, or the same for processing and embedding? > > Paraplast, Surgipath, Shandon ???? > > ------------------------------ Message: 10 Date: Fri, 2 May 2008 10:59:19 +0400 From: "Anne van Binsbergen" Subject: Re: [Histonet] Paraffin types To: "Gayle Callis" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 one size fits all - tissue-tek works for me - been using it for over 10 years now - in all the labs i have managed Annie 2008/5/2 Gayle Callis : > Same paraffin for infiltration and embedding. Our choice is especially > good for bone because it is a harder paraffin and works well for soft > tissues even very thin sections i.g. 1 to 2 um. Since we do a great deal of > bone work, this has been a good choice. Tissue Prep 2 from ThermoFisher > Scientific. > > We have used the Surgipath combination infiltration media followed by > their embedding media with great success. > > Gayle M. Callis > HTL/HT/MT(ASCP) > Bozeman MT 59715 > > > ----- Original Message ----- From: "Joyce Cline" > To: > Sent: Thursday, May 01, 2008 3:06 PM > Subject: [Histonet] Paraffin types > > > What type of paraffin is everyone using? Separate paraffin for > > processing and embedding, or the same for processing and embedding? > > > > Paraplast, Surgipath, Shandon ???? > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne (van Binsbergen) Hope Abu Dhabi UAE ------------------------------ Message: 11 Date: Fri, 2 May 2008 21:12:27 +1000 From: "Piero Nelva" Subject: Re: [Histonet] Paraffin types Cc: Message-ID: <004e01c8ac45$67c761e0$5c72be7c@pentium4> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original I've only ever used the same wax for both processes. Paraplast is the current (>10 years) favorite. Piero ----- Original Message ----- From: "Anne van Binsbergen" To: "Gayle Callis" Cc: Sent: Friday, May 02, 2008 4:59 PM Subject: Re: [Histonet] Paraffin types > one size fits all - tissue-tek works for me - been using it for over 10 > years now - in all the labs i have managed > Annie > > 2008/5/2 Gayle Callis : > >> Same paraffin for infiltration and embedding. Our choice is especially >> good for bone because it is a harder paraffin and works well for soft >> tissues even very thin sections i.g. 1 to 2 um. Since we do a great deal >> of >> bone work, this has been a good choice. Tissue Prep 2 from >> ThermoFisher >> Scientific. >> >> We have used the Surgipath combination infiltration media followed by >> their embedding media with great success. >> >> Gayle M. Callis >> HTL/HT/MT(ASCP) >> Bozeman MT 59715 >> >> >> ----- Original Message ----- From: "Joyce Cline" >> To: >> Sent: Thursday, May 01, 2008 3:06 PM >> Subject: [Histonet] Paraffin types >> >> >> What type of paraffin is everyone using? Separate paraffin for >> > processing and embedding, or the same for processing and embedding? >> > >> > Paraplast, Surgipath, Shandon ???? >> > >> > >> > >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > Anne (van Binsbergen) Hope > Abu Dhabi > UAE > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG. > Version: 7.5.524 / Virus Database: 269.23.7/1410 - Release Date: 5/1/2008 > 5:30 PM > > ------------------------------ Message: 12 Date: Fri, 2 May 2008 08:14:11 -0400 From: Teri.Hallada@midmichigan.org Subject: [Histonet] Ventanna Giemsa To: histonet@pathology.swmed.edu Message-ID: <8839B08E3ED7364E8CBBD53882C984D50994CC67@MAILSRV01.midmichigan.net> Content-Type: text/plain; charset=us-ascii We are having trouble with our Ventanna Giemsa stain for bone marrows. The cells either fall off the slide or explode. Anyone out there willing to share there procedure or have any suggestions? Teresa Hallada BS, MT/CT (ASCP) Lead Cytotechnologist MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. ------------------------------ Message: 13 Date: Fri, 2 May 2008 08:18:42 -0500 From: "Mike Pence" Subject: RE: [Histonet] Ventanna Giemsa To: , Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A380D@IS-E2K3.grhs.net> Content-Type: text/plain; charset="us-ascii" I could never get this stain to work for us and we went back to manual staining. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Teri.Hallada@midmichigan.org Sent: Friday, May 02, 2008 7:14 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Ventanna Giemsa We are having trouble with our Ventanna Giemsa stain for bone marrows. The cells either fall off the slide or explode. Anyone out there willing to share there procedure or have any suggestions? Teresa Hallada BS, MT/CT (ASCP) Lead Cytotechnologist MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Fri, 2 May 2008 09:56:01 -0400 From: "Weber, Susan (VHACLE)" Subject: RE: [Histonet] Paraffin types To: "Joyce Cline" , Message-ID: <16C83872A53F4346AA9C3A18E3A3AAB903F76D92@VHAV10MSGA1.v10.med.va.gov> Content-Type: text/plain; charset="us-ascii" We are using Surgipath Blue Ribbon Tissue Embedding/Infiltration Medium. As the name implies, we use only this one paraffin for both. Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Thursday, May 01, 2008 5:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin types What type of paraffin is everyone using? Separate paraffin for processing and embedding, or the same for processing and embedding? Paraplast, Surgipath, Shandon ???? ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Fri, 2 May 2008 11:31:33 -0400 From: "Tom McNemar" Subject: [Histonet] DRS 60 jackpot! To: Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F568@lmhsmail.lmhealth.org> Content-Type: text/plain; charset="iso-8859-1" Rummaging around and low and behold.... I found 10-12 plastic staining dishes for the Sakura DRS 60! I know that some of these workhorses are still around and that you probably can't get dishes for them anymore, so.... Send me your fedex number and address and I'll ship them out to anyone who wants them. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ------------------------------ Message: 16 Date: Fri, 2 May 2008 08:48:53 -0700 (PDT) From: Schaundra Walton Subject: [Histonet] RE:Pneumocystis controls To: Histonet Message-ID: <661422.27682.qm@web58909.mail.re1.yahoo.com> Content-Type: text/plain; charset="us-ascii" We just recently ordered these controls slides from Mercedes Medical. [1]www.mercedesmedical.com We were having trouble getting slides from our former supplier and Mercedes' prices were pretty comparable. Hope this helps! Schaundra Walton BS, HTL(ASCP) Histology Supervisor Swedish American Hospital Rockford, IL -----Original Message----- Hoping someone knows of a supplier of pneumocystis TISSUE control slides? Thanks Nancy Rutledge _________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. [2]Try it now. References 1. http://www.mercedesmedical.com/ 2. http://us.rd.yahoo.com/evt=51733/*http://mobile.yahoo.com/;_ylt=Ahu06i62sR8H DtDypao8Wcj9tAcJ ------------------------------ Message: 17 Date: Fri, 2 May 2008 11:01:28 -0500 From: "Orr, Rebecca" Subject: [Histonet] IL-1 IL-6 Tnf alpha help To: Message-ID: Content-Type: text/plain; charset="us-ascii" I everyone, I have a researcher who is looking for a lab that will run these antibodies on 24hr Urines. I'm not sure how many samples he has, but if anyone has a reference lab they could recommend I'd be obliged. Mayo does some of these but only on blood/plasma. I'm thinking the specimen type will be the issue. Thanks and let's all have a great weekend. Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 ------------------------------ Message: 18 Date: Fri, 2 May 2008 09:30:57 -0700 From: "Masterson_John" Subject: [Histonet] (no subject) To: "Orr, Rebecca" , Message-ID: <0C58C4F16F0B67448318A38041CADE4B01594E0B@IRMAIL133.irvine.allergan.com> Content-Type: text/plain; charset="us-ascii" -----Original Message----- Hi Histonetter's, Thanks to all who replied to my last question regarding M.O.M. IHC kits. Does anyone have any wisdom regarding the Bodian stain. I've purchased a kit from a well known vendor but after 4 or 5 attempts I still can't get the section to turn dark tan to brown as described for the protargol step. I've ordered a bottle of pre mixed 1% protargol from another vendor and it doesn't work any better. Any advice? Thanks, John ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 54, Issue 2 *************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From John.Spair <@t> multicare.org Sun May 4 14:02:16 2008 From: John.Spair <@t> multicare.org (John Spair) Date: Sun May 4 14:06:33 2008 Subject: [Histonet] RE: Histonet Digest, Vol 54, Issue 3 References: <6402B5EC2EO47891-01@MMS_multicare.org> Message-ID: <61A9977919846C479389493BAE2517CA0153092D@MHSEXMBX1.multicare.org> Julie: I believe the change occurred due to safety. Toluene and Benzene are highly toxic, stray away from them. Message: 6 Date: Fri, 2 May 2008 14:53:18 -0400 From: "Julie Castell" Subject: [Histonet] Benzene, Toluene & Xylene research To: Message-ID: <002f01c8ac85$c9934980$5cb9dc80$@com> Content-Type: text/plain; charset="us-ascii" I am working on a research project and am reaching out to my fellow "histoneters" for help. Does anyone know why the histology process changed from using benzene to toluene and now xylene? How did it transition? Kiki Jude Leeman Laboratories Findlay, Oh "MMS " made the following annotations. ------------------------------------------------------------------------------ NOTICE: This e-mail and the attachments hereto, if any, may contain privileged and/or confidential information. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, you are hereby notified that any examination, distribution or copying of this e-mail and the attachments hereto, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by email or telephone and permanently delete this e-mail and the attachments hereto, if any, and destroy any printout thereof. MultiCare Health System, Tacoma, WA 98415 (253) 403-1000. ============================================================================== From annigyg <@t> gmail.com Sun May 4 23:05:00 2008 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Sun May 4 23:05:09 2008 Subject: [Histonet] Grossing Stations In-Reply-To: <797735aa0805021011i4cef928aga3cc0a8c3e19b760@mail.gmail.com> References: <797735aa0805021011i4cef928aga3cc0a8c3e19b760@mail.gmail.com> Message-ID: gross star is the flashy version of the gross senior. we have a senior which extracts OUT of the building - not a recirculator - runs 24/7 and contains any vapours recently a JCIA inspector said he was impressed that we have no fumes at all!! elevating mechanism is great for accomodating the vertically challenged as well as the taller members of staff. sink is a bit small. flushing spouts and various other water supplies are conveniently placed for ease of use. filters are easy to change only one complainit - water supply inlet at the back of the unit has an ultra sensitive pressure sensor and whenever plumbers work on the water supply anywhere in the building, it alters the pressure and this inlet shuts and we have a flood the machine is very heavy and its hard to get behind it to fiddle with the mechanism to adjust so we deal with the floods from time to time a sister hospital has a gross-star and they are very happy good luck Annie 2008/5/2 Griz Willis : > Hi, > Is anyone familiar with either the CSI/Jewett JGP5 or ThermoShandon > Gross-Star or Gross Senior elevating grossing workstations? > We are having a new lab built and would like to hear any complaints or > praises of any of these units. > Thanks. > > Dan > Harborview Medical Center > Pathology > Histology Supervisor > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne (van Binsbergen) Hope Abu Dhabi UAE From jmjohnson34 <@t> hotmail.com Mon May 5 07:45:51 2008 From: jmjohnson34 <@t> hotmail.com (Jennifer Johnson) Date: Mon May 5 07:45:56 2008 Subject: [Histonet] Dye fixative Message-ID: John, After inking tissue for margins, use a mixture of 1 part glacial acetic acid: 5 parts 10% NB formalin: 15 parts distilled water. Let it sit for a minute then blot with tissue and place in your preferred fixative. The dye will not run or muddy up your solutions in the tissue processor. Hope this helps, Jennifer Johnson, HTL (ASCP) _________________________________________________________________ Windows Live SkyDrive lets you share files with faraway friends. http://www.windowslive.com/skydrive/overview.html?ocid=TXT_TAGLM_WL_Refresh_skydrive_052008 From Heather.D.Renko <@t> osfhealthcare.org Mon May 5 08:12:05 2008 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Mon May 5 08:12:26 2008 Subject: [Histonet] Re: pneumocystis controls Message-ID: <40026EDDE64CDA47AB382C52619ACD3C06612DEE@pmc-rfd-mx01.intranet.osfnet.org> Best practice is to find a positive case of your own but, in theory not always easy to come by. We purchase our Pneumocystis controls from American Master Tech Scientific 209-368-4031. They have excellent people and good quality control which is worth its weight in gold. We have never had a problem and our pathologists actually prefer them over home grown. Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From hej01 <@t> health.state.ny.us Mon May 5 09:12:25 2008 From: hej01 <@t> health.state.ny.us (Helen E Johnson) Date: Mon May 5 09:12:31 2008 Subject: [Histonet] metal mesh gloves Message-ID: Hi Histonetters, Can you please recommend a vendor that sells a good pair of metal mesh gloves. Thanks. Helen Johnson (hej01@health.state.ny.us) IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. From gayle.callis <@t> bresnan.net Mon May 5 09:40:05 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Mon May 5 09:40:12 2008 Subject: [Histonet] metal mesh gloves References: Message-ID: <003601c8aebd$eab065a0$6501a8c0@DHXTS541> Helen, Try to locate a meatcutter/butchers supply house on internet. We used to buy cut resistant gloves from Koch, now under another name/management (?). Just Google meatcutters metal mesh gloves and you will find what you need. The joy of this, their gloves are much cheaper than buying them from a scientific vendor to do the same job. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT ----- Original Message ----- From: "Helen E Johnson" To: Sent: Monday, May 05, 2008 8:12 AM Subject: [Histonet] metal mesh gloves > > Hi Histonetters, > Can you please recommend a vendor that sells a good pair of metal mesh > gloves. > Thanks. Helen Johnson > (hej01@health.state.ny.us) > > > IMPORTANT NOTICE: This e-mail and any attachments may contain > confidential or sensitive information which is, or may be, legally > privileged or otherwise protected by law from further disclosure. It is > intended only for the addressee. If you received this in error or from > someone who was not authorized to send it to you, please do not > distribute, copy or use it or any attachments. Please notify the sender > immediately by reply e-mail and delete this from your system. Thank you > for your cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Mon May 5 09:49:12 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Mon May 5 09:49:19 2008 Subject: [Histonet] Trying to find Teresa Flores Message-ID: <005601c8aebf$2eb99540$6501a8c0@DHXTS541> Histonetters, I am trying to locate Teresa Flores from the New Orleans area (maybe formerly from there). Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 From tshrobertson <@t> yahoo.com Mon May 5 11:39:53 2008 From: tshrobertson <@t> yahoo.com (Teisha Robertson) Date: Mon May 5 11:40:01 2008 Subject: [Histonet] cryoprotection Message-ID: <977462.62375.qm@web62515.mail.re1.yahoo.com> i am trying to cryoprotect the mouse brain. i place the brain in 10% formalin,70%ETOH and 30% sucrose. can you please tell me what i need to do to prevent holes in my sample? --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From Jerry <@t> ralambusa.com Mon May 5 12:13:35 2008 From: Jerry <@t> ralambusa.com (Jerry Helisek) Date: Mon May 5 12:13:40 2008 Subject: [Histonet] RE: metal mesh gloves Message-ID: <3855F92002259948A66A8CA2D16E3A4F05B029@server.ralambusa.com> Helen, I have a restaurant here in Durham, NC and we purchase mesh gloves at our local Restaurant Supply Store, just do a google search or next time you go into a "local" restaurant ask the owner/manager (the only reason I emphasize "local" is that, "local" proprietors are more likely to purchase locally rather than shop from their larger parent companies designated suppliers...). They are a must for cleaning/assembly of sharp equipment. Good luck! ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ ------------------------------ Message: 5 Date: Mon, 5 May 2008 10:12:25 -0400 From: Helen E Johnson Subject: [Histonet] metal mesh gloves To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Hi Histonetters, Can you please recommend a vendor that sells a good pair of metal mesh gloves. Thanks. Helen Johnson (hej01@health.state.ny.us) IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. From pmarcum <@t> vet.upenn.edu Mon May 5 12:55:57 2008 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Mon May 5 12:56:00 2008 Subject: [Histonet] Glenda Hood Please Call Me Message-ID: <6.2.5.6.2.20080505135440.01c97170@vet.upenn.edu> Hi All, I am sorry but I need to contact Glenda Hood or have her contact me. We have a microtome for her project. Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From pmarcum <@t> vet.upenn.edu Mon May 5 12:56:02 2008 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Mon May 5 12:56:06 2008 Subject: [Histonet] (no subject) Message-ID: <6.2.5.6.2.20080505135433.01c859c0@vet.upenn.edu> Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From Janet.Bonner <@t> FLHOSP.ORG Mon May 5 13:11:44 2008 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Mon May 5 13:12:23 2008 Subject: [Histonet] RE: metal mesh gloves References: <3855F92002259948A66A8CA2D16E3A4F05B029@server.ralambusa.com> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F260E@fhosxchmb006.ADVENTISTCORP.NET> OR...Lab Safety Supply. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jerry Helisek Sent: Mon 5/5/2008 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: metal mesh gloves Helen, I have a restaurant here in Durham, NC and we purchase mesh gloves at our local Restaurant Supply Store, just do a google search or next time you go into a "local" restaurant ask the owner/manager (the only reason I emphasize "local" is that, "local" proprietors are more likely to purchase locally rather than shop from their larger parent companies designated suppliers...). They are a must for cleaning/assembly of sharp equipment. Good luck! ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ ------------------------------ Message: 5 Date: Mon, 5 May 2008 10:12:25 -0400 From: Helen E Johnson Subject: [Histonet] metal mesh gloves To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Hi Histonetters, Can you please recommend a vendor that sells a good pair of metal mesh gloves. Thanks. Helen Johnson (hej01@health.state.ny.us) IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From djohnson <@t> mercedesmedical.com Mon May 5 13:17:04 2008 From: djohnson <@t> mercedesmedical.com (Dave Johnson) Date: Mon May 5 13:17:10 2008 Subject: [Histonet] RE: metal mesh gloves Message-ID: We carry them Dave Johnson Mercedes Medical 800-331-2716 From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonner, Janet Sent: Monday, May 05, 2008 2:12 PM To: Jerry Helisek; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: metal mesh gloves OR...Lab Safety Supply. From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jerry Helisek Sent: Mon 5/5/2008 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: metal mesh gloves I have a restaurant here in Durham, NC and we purchase mesh gloves at our local Restaurant Supply Store, just do a google search or next time you go into a "local" restaurant ask the owner/manager (the only reason I emphasize "local" is that, "local" proprietors are more likely to purchase locally rather than shop from their larger parent companies designated suppliers...). They are a must for cleaning/assembly of sharp equipment. Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 Date: Mon, 5 May 2008 10:12:25 -0400 From: Helen E Johnson Subject: [Histonet] metal mesh gloves To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Hi Histonetters, Can you please recommend a vendor that sells a good pair of metal mesh gloves. Thanks. Helen Johnson (hej01@health.state.ny.us) From JWeems <@t> sjha.org Mon May 5 13:40:47 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Mon May 5 13:40:48 2008 Subject: [Histonet] Cryostat to donate Message-ID: <982A0A9461F9BF438C7B19A6E425A38312AA87@ITSSSXM01V6.one.ads.che.org> Does anyone have a working cryostat that they could donate to Doctors Without Borders? I don't know details, but will find out if anyone could help. Thanks and happy Monday! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From barbaraaalbert <@t> yahoo.com Mon May 5 16:45:37 2008 From: barbaraaalbert <@t> yahoo.com (Barbara Albert) Date: Mon May 5 16:45:44 2008 Subject: [Histonet] California labs-have you moved recently? Message-ID: <707321.90101.qm@web63711.mail.re1.yahoo.com> Hi all, We moving soon and would sincerely appreciate any tips or warnings of any pitfalls. We are partiularly interestes in the procedure used to validate your equipment. Thanks, Barbara Albert UCSF Medical Center San Francisco --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From AnthonyH <@t> chw.edu.au Mon May 5 18:25:33 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon May 5 18:25:46 2008 Subject: [Histonet] (no subject) In-Reply-To: <6.2.5.6.2.20080505135433.01c859c0@vet.upenn.edu> Message-ID: Thanks!! ?? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Tuesday, 6 May 2008 3:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From kadamsplw <@t> gmail.com Tue May 6 08:00:54 2008 From: kadamsplw <@t> gmail.com (karen adams) Date: Tue May 6 08:01:07 2008 Subject: [Histonet] B-plus question Message-ID: Hello all....we are changing bone marrow fixative from formalin to B-plus. If we fix for the required time in B-plu and then process on the VIP w/ the other specimens using formalin are there any effects on the tissue going from B-plus to formalin?? Thank you in advance -- Karen Adams Supervisor Pathology Laboratories West 9303 Park West Blvd. Knoxville, TN 37923 kadamsplw@gmail.com From rjbuesa <@t> yahoo.com Tue May 6 08:15:30 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue May 6 08:15:35 2008 Subject: [Histonet] B-plus question In-Reply-To: Message-ID: <621028.53193.qm@web65713.mail.ac4.yahoo.com> No, because the tissue is supposed to be fixed already. Ren? J. karen adams wrote: Hello all....we are changing bone marrow fixative from formalin to B-plus. If we fix for the required time in B-plu and then process on the VIP w/ the other specimens using formalin are there any effects on the tissue going from B-plus to formalin?? Thank you in advance -- Karen Adams Supervisor Pathology Laboratories West 9303 Park West Blvd. Knoxville, TN 37923 kadamsplw@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From histology.bc <@t> shaw.ca Tue May 6 08:52:32 2008 From: histology.bc <@t> shaw.ca (Paul Bradbury) Date: Tue May 6 08:46:52 2008 Subject: [Histonet] B-plus question In-Reply-To: References: Message-ID: <482062A0.4080500@shaw.ca> Hi Karen, I have been using the same sequence of reagents for several years with great success. We routinely fix bone marrow cores for 3 hours in B-plus, rinse in water, decalcify, rinse again, and put the cassette in with all the other tissues for processing. B-plus contains formaldehyde anyway, so your are not introducing a different reagent when you transfer them to formalin during processing. B-plus gives much better cytological detail than formalin. Nuclear detail is crisper, granules are better preserved, hemosiderin is not effected. CD-3, CD-20, kappa and lambda, etc. all work beautifully after B-plus. It also has the advantage of not producing an fixation artefact pigment like B-5 does. I think you will be happy with your decision to change. Paul Bradbury Kamloops, Canada karen adams wrote: > Hello all....we are changing bone marrow fixative from formalin to B-plus. > If we fix for the required time in B-plu and then process on the VIP w/ the > other specimens using formalin are there any effects on the tissue going > from B-plus to formalin?? > Thank you in advance > From Linke_Noelle <@t> Allergan.com Tue May 6 09:24:37 2008 From: Linke_Noelle <@t> Allergan.com (Linke_Noelle) Date: Tue May 6 09:25:41 2008 Subject: [Histonet] GLP question In-Reply-To: <2a926e3f0805021127k8fa51an54e8e001dbefd54d@mail.gmail.com> References: <2a926e3f0805021127k8fa51an54e8e001dbefd54d@mail.gmail.com> Message-ID: <5C3DA4BE34AA0641BAA10A7C1478B60527D204@IRMAIL132.irvine.allergan.com> Hi everyone, I have a question for all of the GLP compliant folks out there. How do you handle recuts that you do prior to giving slides to the pathologist? For example, you stain on Monday, look at your slides and find you are missing something like the mammary gland. You recut and stain on Tuesday....how do you document that? Do you keep your original slide and archive it? I thank you in advance for any help! Noelle No?lle Linke, MS, HTL(ASCP)QIHC Allergan, Inc 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5568 From mpence <@t> grhs.net Tue May 6 09:39:17 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Tue May 6 09:39:24 2008 Subject: [Histonet] Proper Grossing Attire Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3816@IS-E2K3.grhs.net> I would like to hear everyone's opinions on what the proper PPE is for "grossing in" specimens. Two levels of grossing in are: 1) dictating and submit specimen - limited to bisecting a punch bx and 2) dictating, describing, cutting, taking selected sections - some judgment and advanced training required ie. colons-placentas. My question is: "Is gloves alone appropriate attire for the first level? What do you wear when grossing at the second level? You may choose to reply off line as to save yourself much "flaming" I sure. Thanks in advance, Mike From c.nixon <@t> beatson.gla.ac.uk Tue May 6 10:13:32 2008 From: c.nixon <@t> beatson.gla.ac.uk (Colin Nixon) Date: Tue May 6 10:13:40 2008 Subject: [Histonet] pan cytokeratin markers Message-ID: <0E5DFBD0E5E27443A0987F7664787163018D2B10@exchange-be4.centre.ad.gla.ac.uk> Can anyone recommend a good pan cytokeratin antibody for use on mouse tissue for paraffin fixed embedded immunohistochemistry and if they can what retrieval method/dilution etc that they use? Thanks, Colin From doug <@t> ppspath.com Tue May 6 11:30:39 2008 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue May 6 10:32:00 2008 Subject: [Histonet] Goodbye and Thanks Message-ID: I wanted to thank all that participate on histonet and for making this such a valuable asset. I have enjoyed it very much. I will be departing my current position on May 23rd. I will be taking a position with an IHC company (technical side) that will have me in the sun of South Florida. I will be reduced to lurking histonet when I have the chance. Keep up the great work and thanks for all that you do. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. From BFicher <@t> chomp.org Tue May 6 11:21:54 2008 From: BFicher <@t> chomp.org (Fischer, R. B) Date: Tue May 6 11:22:01 2008 Subject: [Histonet] RINSING IN TAP WATER AFTER DECAL Message-ID: We have been using "RDO" and "CAL-X" decal solutions for some time now. After the specified times in the decal solutions, the specimens are rinsed in running tap water for about 10 minutes before being placed back in 10%NBF for routine processing. We were rinsing to get rid of the nitric acid. My questions are (1) Is this rinse step necessary? If it is, (2) How long is an adequate time for rinsing?(3) How are other labs handling the specimen post decal, and before processing? R.Brian Fischer Histology Lead Tech Community Hospital of the Monterey Peninsula PO Box HH Monterey Ca. 93942 831-625-4791 Fax: 831-6583683 Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. From galinadeyneko <@t> yahoo.com Tue May 6 11:28:19 2008 From: galinadeyneko <@t> yahoo.com (Galina Deyneko) Date: Tue May 6 11:28:22 2008 Subject: [Histonet] gross-section Message-ID: <397885.31237.qm@web33101.mail.mud.yahoo.com> Dear Colleagues Could you please advise the sources like manuals, textbooks or websites for gross-sectioning/ sampling of the organs of big mammalians ( we work with rabbits, monkeys). I think human gross-sectioning information also useful. Thank you. galina deyneko novartis,cambridge, ma --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From Heather.D.Renko <@t> osfhealthcare.org Tue May 6 12:03:03 2008 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Tue May 6 12:03:19 2008 Subject: [Histonet] mycobacterium tuberculosis and fixation Message-ID: <40026EDDE64CDA47AB382C52619ACD3C0A09DF07@pmc-rfd-mx01.intranet.osfnet.org> Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From mpence <@t> grhs.net Tue May 6 11:51:01 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Tue May 6 12:09:38 2008 Subject: [Histonet] RINSING IN TAP WATER AFTER DECAL In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3817@IS-E2K3.grhs.net> We do the same as you describe. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fischer, R. B Sent: Tuesday, May 06, 2008 11:22 AM To: histonet@lists.utsouthwestern.edu Cc: Delcambre, Linda V Subject: [Histonet] RINSING IN TAP WATER AFTER DECAL We have been using "RDO" and "CAL-X" decal solutions for some time now. After the specified times in the decal solutions, the specimens are rinsed in running tap water for about 10 minutes before being placed back in 10%NBF for routine processing. We were rinsing to get rid of the nitric acid. My questions are (1) Is this rinse step necessary? If it is, (2) How long is an adequate time for rinsing?(3) How are other labs handling the specimen post decal, and before processing? R.Brian Fischer Histology Lead Tech Community Hospital of the Monterey Peninsula PO Box HH Monterey Ca. 93942 831-625-4791 Fax: 831-6583683 Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Eric.Mahoney <@t> cchmc.org Tue May 6 12:12:43 2008 From: Eric.Mahoney <@t> cchmc.org (Eric Mahoney) Date: Tue May 6 12:13:14 2008 Subject: [Histonet] Re: RINSING IN TAP WATER AFTER DECAL Message-ID: <4820594B0200009700015F49@n6mcgw16.cchmc.org> I have used Cal-EX by Fisher before. After decal. I ran my samples in running tap water for 4 hours. I am decal. vertebral bone and my sections and HE staining came out pretty well. I would not skip this step. >>> 05/06/08 1:02 PM >>> Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: metal mesh gloves (Jerry Helisek) 2. Glenda Hood Please Call Me (Pamela Marcum) 3. (no subject) (Pamela Marcum) 4. RE: RE: metal mesh gloves (Bonner, Janet) 5. RE: RE: metal mesh gloves (Dave Johnson) 6. Cryostat to donate (Weems, Joyce) 7. California labs-have you moved recently? (Barbara Albert) 8. RE: (no subject) (Tony Henwood) 9. B-plus question (karen adams) 10. Re: B-plus question (Rene J Buesa) 11. Re: B-plus question (Paul Bradbury) 12. GLP question (Linke_Noelle) 13. Proper Grossing Attire (Mike Pence) 14. pan cytokeratin markers (Colin Nixon) 15. Goodbye and Thanks (Douglas D Deltour) 16. RINSING IN TAP WATER AFTER DECAL (Fischer, R. B) 17. gross-section (Galina Deyneko) ---------------------------------------------------------------------- Message: 1 Date: Mon, 5 May 2008 13:13:35 -0400 From: "Jerry Helisek" Subject: [Histonet] RE: metal mesh gloves To: Message-ID: <3855F92002259948A66A8CA2D16E3A4F05B029@server.ralambusa.com> Content-Type: text/plain; charset="us-ascii" Helen, I have a restaurant here in Durham, NC and we purchase mesh gloves at our local Restaurant Supply Store, just do a google search or next time you go into a "local" restaurant ask the owner/manager (the only reason I emphasize "local" is that, "local" proprietors are more likely to purchase locally rather than shop from their larger parent companies designated suppliers...). They are a must for cleaning/assembly of sharp equipment. Good luck! ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ ------------------------------ Message: 5 Date: Mon, 5 May 2008 10:12:25 -0400 From: Helen E Johnson Subject: [Histonet] metal mesh gloves To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Hi Histonetters, Can you please recommend a vendor that sells a good pair of metal mesh gloves. Thanks. Helen Johnson (hej01@health.state.ny.us) IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. ------------------------------ Message: 2 Date: Mon, 05 May 2008 13:55:57 -0400 From: Pamela Marcum Subject: [Histonet] Glenda Hood Please Call Me To: histonet@lists.utsouthwestern.edu Message-ID: <6.2.5.6.2.20080505135440.01c97170@vet.upe Hi All, I am sorry but I need to contact Glenda Hood or have her contact me. We have a microtome for her project. Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu ------------------------------ Message: 3 Date: Mon, 05 May 2008 13:56:02 -0400 From: Pamela Marcum Subject: [Histonet] (no subject) To: histonet@lists.utsouthwestern.edu Message-ID: <6.2.5.6.2.20080505135433.01c859c0@vet.upenn.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu ------------------------------ Message: 4 Date: Mon, 5 May 2008 14:11:44 -0400 From: "Bonner, Janet" Subject: RE: [Histonet] RE: metal mesh gloves To: "Jerry Helisek" , histonet@lists.utsouthwestern.edu Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F260E@fhosxchmb006.ADVENTISTCORP.NET> Content-Type: text/plain; charset=iso-8859-1 OR...Lab Safety Supply. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jerry Helisek Sent: Mon 5/5/2008 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: metal mesh gloves Helen, I have a restaurant here in Durham, NC and we purchase mesh gloves at our local Restaurant Supply Store, just do a google search or next time you go into a "local" restaurant ask the owner/manager (the only reason I emphasize "local" is that, "local" proprietors are more likely to purchase locally rather than shop from their larger parent companies designated suppliers...). They are a must for cleaning/assembly of sharp equipment. Good luck! ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ ------------------------------ Message: 5 Date: Mon, 5 May 2008 10:12:25 -0400 From: Helen E Johnson Subject: [Histonet] metal mesh gloves To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Hi Histonetters, Can you please recommend a vendor that sells a good pair of metal mesh gloves. Thanks. Helen Johnson (hej01@health.state.ny.us) IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= ------------------------------ Message: 5 Date: Mon, 5 May 2008 14:17:04 -0400 From: "Dave Johnson" Subject: RE: [Histonet] RE: metal mesh gloves To: Message-ID: Content-Type: text/plain; charset="us-ascii" We carry them Dave Johnson Mercedes Medical 800-331-2716 From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonner, Janet Sent: Monday, May 05, 2008 2:12 PM To: Jerry Helisek; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: metal mesh gloves OR...Lab Safety Supply. From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jerry Helisek Sent: Mon 5/5/2008 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: metal mesh gloves I have a restaurant here in Durham, NC and we purchase mesh gloves at our local Restaurant Supply Store, just do a google search or next time you go into a "local" restaurant ask the owner/manager (the only reason I emphasize "local" is that, "local" proprietors are more likely to purchase locally rather than shop from their larger parent companies designated suppliers...). They are a must for cleaning/assembly of sharp equipment. Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 Date: Mon, 5 May 2008 10:12:25 -0400 From: Helen E Johnson Subject: [Histonet] metal mesh gloves To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Hi Histonetters, Can you please recommend a vendor that sells a good pair of metal mesh gloves. Thanks. Helen Johnson (hej01@health.state.ny.us) ------------------------------ Message: 6 Date: Mon, 5 May 2008 14:40:47 -0400 From: "Weems, Joyce" Subject: [Histonet] Cryostat to donate To: Message-ID: <982A0A9461F9BF438C7B19A6E425A38312AA87@ITSSSXM01V6.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" Does anyone have a working cryostat that they could donate to Doctors Without Borders? I don't know details, but will find out if anyone could help. Thanks and happy Monday! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ------------------------------ Message: 7 Date: Mon, 5 May 2008 14:45:37 -0700 (PDT) From: Barbara Albert Subject: [Histonet] California labs-have you moved recently? To: histonet@lists.utsouthwestern.edu Message-ID: <707321.90101.qm@web63711.mail.re1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi all, We moving soon and would sincerely appreciate any tips or warnings of any pitfalls. We are partiularly interestes in the procedure used to validate your equipment. Thanks, Barbara Albert UCSF Medical Center San Francisco ------------------------------------------------------------- Message: 8 Date: Tue, 6 May 2008 09:25:33 +1000 From: "Tony Henwood" Subject: RE: [Histonet] (no subject) To: "Pamela Marcum" , Message-ID: Content-Type: text/plain; charset="us-ascii" Thanks!! ?? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Tuesday, 6 May 2008 3:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 9 Date: Tue, 6 May 2008 09:00:54 -0400 From: "karen adams" Subject: [Histonet] B-plus question To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hello all....we are changing bone marrow fixative from formalin to B-plus. If we fix for the required time in B-plu and then process on the VIP w/ the other specimens using formalin are there any effects on the tissue going from B-plus to formalin?? Thank you in advance -- Karen Adams Supervisor Pathology Laboratories West 9303 Park West Blvd. Knoxville, TN 37923 kadamsplw@gmail.com ------------------------------ Message: 10 Date: Tue, 6 May 2008 06:15:30 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] B-plus question To: karen adams , histonet@lists.utsouthwestern.edu Message-ID: <621028.53193.qm@web65713.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 No, because the tissue is supposed to be fixed already. Ren? J. karen adams wrote: Hello all....we are changing bone marrow fixative from formalin to B-plus. If we fix for the required time in B-plu and then process on the VIP w/ the other specimens using formalin are there any effects on the tissue going from B-plus to formalin?? Thank you in advance -- Karen Adams Supervisor Pathology Laboratories West 9303 Park West Blvd. Knoxville, TN 37923 kadamsplw@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. ------------------------------ Message: 11 Date: Tue, 06 May 2008 06:52:32 -0700 FromTo: karen adams , HistoNet Server Message-ID: <482062A0.4080500@shaw.ca> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hi Karen, I have been using the same sequence of reagents for several years with great success. We routinely fix bone marrow cores for 3 hours in B-plus, rinse in water, decalcify, rinse again, and put the cassette in with all the other tissues for processing. B-plus contains formaldehyde anyway, so your are not introducing a different reagent when you transfer them to formalin during processing. B-plus gives much better cytological detail than formalin. Nuclear detail is crisper, granules are better preserved, hemosiderin is not effected. CD-3, CD-20, kappa and lambda, etc. all work beautifully after B-plus. It also has the advantage of not producing an fixation artefact pigment like B-5 does. I think you will be happy with your decision to change. Paul Bradbury Kamloops, Canada karen adams wrote: > Hello all....we are changing bone marrow fixative from formalin to B-plus. > If we fix for the required time in B-plu and then process on the VIP w/ the > other specimens using formalin are there any effects on the tissue going > from B-plus to formalin?? > Thank you in advance > ------------------------------ Message: 12 Date: Tue, 6 May 2008 07:24:37 -0700 From: "Linke_Noelle" Subject: [Histonet] GLP question To: Message-ID: <5C3DA4BE34AA0641BAA10A7C1478B60527D204@IRMAIL132.irvine.allergan.com> Content-Type: text/plain; charset="iso-8859-1" Hi everyone, I have a question for all of the GLP compliant folks out there. How do you handle recuts that you do prior to giving slides to the pathologist? For example, you stain on Monday, look at your slides and find you are missing something like the mammary gland. You recut and stain on Tuesday....how do you document that? Do you keep your original slide and archive it? I thank you in advance for any help! Noelle No?lle Linke, MS, HTL(ASCP)QIHC Allergan, Inc 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5568 ------------------------------ Message: 13 Date: Tue, 6 May 2008 09:39:17 -0500 From: "Mike Pence" Subject: [Histonet] Proper Grossing Attire To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3816@IS-E2K3.grhs.net> Content-Type: text/plain; charset="us-ascii" I would like to hear everyone's opinions on what the proper PPE is for "grossing in" specimens. Two levels of grossing in are: 1) dictating and submit specimen - limited to bisecting a punch bx and 2) dictating, describing, cutting, taking selected sections - some judgment and advanced training required ie. colons-placentas. My question is: "Is gloves alone appropriate attire for the first level? What do you wear when grossing at the second level? You may choose to reply off line as to save yourself much "flaming" I sure. Thanks in advance, Mike ------------------------------ Message: 14 Date: Tue, 6 May 2008 16:13:32 +0100 From: "Colin Nixon" Subject: [Histonet] pan cytokeratin markers To: Message-ID: <0E5DFBD0E5E27443A0987F7664787163018D2B10@exchange-be4.centre.ad.gla.ac.uk> Content-Type: text/plain; charset="iso-8859-1" Can anyone recommend a good pan cytokeratin antibody for use on mouse tissue for paraffin fixed embedded immunohistochemistry and if they can what retrieval method/dilution etc that they use? Thanks, Colin ------------------------------ Message: 15 Date: Tue, 6 May 2008 11:30:39 -0500 From: "Douglas D Deltour" Subject: [Histonet] Goodbye and Thanks To: Message-ID: Content-Type: text/plain; charset="US-ASCII" I wanted to thank all that participate on histonet and for making this such a valuable asset. I have enjoyed it very much. I will be departing my current position on May 23rd. I will be taking a position with an IHC company (technical side) that will have me in the sun of South Florida. I will be reduced to lurking histonet when I have the chance. Keep up the great work and thanks for all that you do. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. ------------------------------ Message: 16 Date: Tue, 6 May 2008 09:21:54 -0700 From: "Fischer, R. B" Subject: [Histonet] RINSING IN TAP WATER AFTER DECAL To: Cc: "Delcambre, Linda V" Message-ID: Content-Type: text/plain; charset="us-ascii" We have been using "RDO" and "CAL-X" decal solutions for some time now. After the specified times in the decal solutions, the specimens are rinsed in running tap water for about 10 minutes before being placed back in 10%NBF for routine processing. We were rinsing to get rid of the nitric acid. My questions are (1) Is this rinse step necessary? If it is, (2) How long is an adequate time for rinsing?(3) How are other labs handling the specimen post decal, and before processing? R.Brian Fischer Histology Lead Tech Community Hospital of the Monterey Peninsula PO Box HH Monterey Ca. 93942 831-625-4791 Fax: 831-6583683 Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. ------------------------------ Message: 17 Date: Tue, 6 May 2008 09:28:19 -0700 (PDT) From: Galina Deyneko Subject: [Histonet] gross-section To: histonet@lists.utsouthwestern.edu Message-ID: <397885.31237.qm@web33101.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Dear Colleagues Could you please advise the sources like manuals, textbooks or websites for gross-sectioning/ sampling of the organs of big mammalians ( we work with rabbits, monkeys). I think human gross-sectioning information also useful. Thank you. galina deyneko novartis,cambridge, ma --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 54, Issue 6 *************************************** From POWELL_SA <@t> Mercer.edu Tue May 6 12:14:04 2008 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Tue May 6 12:19:03 2008 Subject: [Histonet] Reminder Message-ID: <01MUGX0OUXOS003SEM@Macon2.Mercer.edu> I sent this out earlier and some have asked the cost of the workshop, it is FREE. The only cost to you is transportation getting there and of course if you need a hotel room that is the only cost to you. The workshop is free. The information is on our website below. Here is a free workshop with free CEUs for those in the Southeast who can get to Atlanta on May 21st. For more information go to our web site, GSH at www.hiistosearch.com/gsh, click on the education page or click on this link. http://www.histosearch.com/gsh/LeicaSymposium.pdf Shirley Powell GSH Secretary From renafail <@t> bellsouth.net Tue May 6 12:37:15 2008 From: renafail <@t> bellsouth.net (Rena Fail) Date: Tue May 6 12:37:27 2008 Subject: [Histonet] RINSING IN TAP WATER AFTER DECAL In-Reply-To: Message-ID: <000001c8af9f$d3cfa200$0301a8c0@RENAD4YK9B8ABE> The rinse step is necessary between formalin and RDO, the two chemicals should not be combined Rena -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fischer, R. B Sent: Tuesday, May 06, 2008 12:22 PM To: histonet@lists.utsouthwestern.edu Cc: Delcambre, Linda V Subject: [Histonet] RINSING IN TAP WATER AFTER DECAL We have been using "RDO" and "CAL-X" decal solutions for some time now. After the specified times in the decal solutions, the specimens are rinsed in running tap water for about 10 minutes before being placed back in 10%NBF for routine processing. We were rinsing to get rid of the nitric acid. My questions are (1) Is this rinse step necessary? If it is, (2) How long is an adequate time for rinsing?(3) How are other labs handling the specimen post decal, and before processing? R.Brian Fischer Histology Lead Tech Community Hospital of the Monterey Peninsula PO Box HH Monterey Ca. 93942 831-625-4791 Fax: 831-6583683 Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jason.Burrill <@t> crl.com Tue May 6 12:46:31 2008 From: Jason.Burrill <@t> crl.com (Burrill, Jason) Date: Tue May 6 12:47:22 2008 Subject: [Histonet] RE: Proper Grossing Attire Message-ID: <1AD4E907E9B6F648AEF1B3A20A9B0E1EFFD6AE@shr-exch2.na01.crl.com> Hi Mike, Each laboratory has the obligation to perform a hazard assessment of each procedure in their lab, 29CFR 1910.132(d)(2) , and determine what hazards are present and the steps they are taking to minimize or eliminate that those hazards. This must be a written document that employees must sign off on so they know what must be done to ensure their safety while performing that procedure. Additionally the OSHA Formaldehyde standard clearly states in 29CFR 1910.1048(h)(1)(i) that you need to be wearing an impervious apron/gown/lab coat, splash goggles and other protective apparel (i.e. gloves) while working with formaldehyde solutions that contain more than 1% formaldehyde. In our laboratory if someone is in close proximity to someone (e.g. observing the procedure) wearing PPE then they must wear the same PPE as the person performing the procedure. I understand that this is a Microsoft answer, no direct help, but unfortunately all labs are not created equal hence the need for a thorough hazard assessment of each procedure. Regards, Jason Jason Burrill Sr. Manager, Histology and Laboratory Safety Research Animal Diagnostic Services Charles River 251 Ballardvale St Wilmington, MA 01887 Office: 781-222-6152 Fax: 978-988-8793 jason.burrill@crl.com www.criver.com Accelerating Drug Development. Exactly. Notice - This email and any files transmitted with it are confidential and may contain privileged and/or proprietary information. You must not disclose this message to another party without Charles River's express written consent. If you are not the intended recipient you must not copy, distribute or use this email or the information contained in it for any purpose other than to notify us. If you have received this message in error, please notify Charles River immediately, and delete it from your system. From kmerriam2003 <@t> yahoo.com Tue May 6 13:04:50 2008 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Tue May 6 13:04:54 2008 Subject: [Histonet] GLP question Message-ID: <951079.35649.qm@web50306.mail.re2.yahoo.com> It has been a while since I have worked in a GLP lab; but if the slides had not gone to the pathologist yet, the original would have been tossed. Recuts requested by the pathologist were another story entirely. Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: Linke_Noelle To: histonet@lists.utsouthwestern.edu Sent: Tuesday, May 6, 2008 10:24:37 AM Subject: [Histonet] GLP question Hi everyone, I have a question for all of the GLP compliant folks out there. How do you handle recuts that you do prior to giving slides to the pathologist? For example, you stain on Monday, look at your slides and find you are missing something like the mammary gland. You recut and stain on Tuesday....how do you document that? Do you keep your original slide and archive it? I thank you in advance for any help! Noelle No?lle Linke, MS, HTL(ASCP)QIHC Allergan, Inc 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5568 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ From dusko.trajkovic <@t> pfizer.com Tue May 6 14:48:00 2008 From: dusko.trajkovic <@t> pfizer.com (Trajkovic, Dusko) Date: Tue May 6 14:48:10 2008 Subject: [Histonet] GLP question In-Reply-To: <951079.35649.qm@web50306.mail.re2.yahoo.com> Message-ID: <3AD0BD3142459B4E9B12CBEAFF2B89B2070C2617@lajamrexm01.amer.pfizer.com> I am in agreement with Kim. Dusko -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Tuesday, May 06, 2008 11:05 AM To: Linke_Noelle; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] GLP question It has been a while since I have worked in a GLP lab; but if the slides had not gone to the pathologist yet, the original would have been tossed. Recuts requested by the pathologist were another story entirely. Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: Linke_Noelle To: histonet@lists.utsouthwestern.edu Sent: Tuesday, May 6, 2008 10:24:37 AM Subject: [Histonet] GLP question Hi everyone, I have a question for all of the GLP compliant folks out there. How do you handle recuts that you do prior to giving slides to the pathologist? For example, you stain on Monday, look at your slides and find you are missing something like the mammary gland. You recut and stain on Tuesday....how do you document that? Do you keep your original slide and archive it? I thank you in advance for any help! Noelle No?lle Linke, MS, HTL(ASCP)QIHC Allergan, Inc 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5568 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anitaibsc <@t> aol.com Tue May 6 15:50:29 2008 From: anitaibsc <@t> aol.com (anitaibsc@aol.com) Date: Tue May 6 15:51:11 2008 Subject: [Histonet] IHC products / replacement for Biomeda products Message-ID: <8CA7DD1F420D8E1-1174-17BF@webmail-nd21.sysops.aol.com> Hello All, This is to let you know that if you need replacement for Biomeda products like mounting media, antibodies, or buffers, they are available now. Contact us at anitaibsc@aol.com. Thank you. Anita Hingorani VP Marketing/Sales ImmunoBioScience Corp. 650-343-IBSC From elockman <@t> apsemail.com Tue May 6 16:43:42 2008 From: elockman <@t> apsemail.com (Emily Lockman) Date: Tue May 6 16:44:00 2008 Subject: [Histonet] GLP question In-Reply-To: <3AD0BD3142459B4E9B12CBEAFF2B89B2070C2617@lajamrexm01.amer.pfizer.com> References: <951079.35649.qm@web50306.mail.re2.yahoo.com> <3AD0BD3142459B4E9B12CBEAFF2B89B2070C2617@lajamrexm01.amer.pfizer.com> Message-ID: <037BDA8D37760D49A2A23D1C877EA8C95E0511@apsdc01.aps.dom> I currently work in a GLP compliant lab and we throw the original. Since the Pathologist won't see it, it does not need to be kept as "raw data". When a Path does request a recut, we label it as recut and keep the original. Hope this helps! Emily Emily M. Lockman, HT (ASCP) Histotechnologist II, Pathology Services American Preclinical Services, LLC (APS) 8945 Evergreen Boulevard Minneapolis, MN 55433 Phone: 763-717-7990 Fax: 763-717-2042 elockman@apsemail.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Trajkovic, Dusko Sent: Tuesday, May 06, 2008 2:48 PM To: Kim Merriam; Linke_Noelle; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] GLP question I am in agreement with Kim. Dusko -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Tuesday, May 06, 2008 11:05 AM To: Linke_Noelle; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] GLP question It has been a while since I have worked in a GLP lab; but if the slides had not gone to the pathologist yet, the original would have been tossed. Recuts requested by the pathologist were another story entirely. Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: Linke_Noelle To: histonet@lists.utsouthwestern.edu Sent: Tuesday, May 6, 2008 10:24:37 AM Subject: [Histonet] GLP question Hi everyone, I have a question for all of the GLP compliant folks out there. How do you handle recuts that you do prior to giving slides to the pathologist? For example, you stain on Monday, look at your slides and find you are missing something like the mammary gland. You recut and stain on Tuesday....how do you document that? Do you keep your original slide and archive it? I thank you in advance for any help! Noelle No?lle Linke, MS, HTL(ASCP)QIHC Allergan, Inc 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5568 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Adam.T.Anthony <@t> kp.org Tue May 6 18:01:48 2008 From: Adam.T.Anthony <@t> kp.org (Adam.T.Anthony@kp.org) Date: Tue May 6 18:02:40 2008 Subject: [Histonet] Sporothrichosis detection by IHC Message-ID: Anyone know of a product commercially available for detection of sporothrichosis by IHC? Thanks, Adam Anthony From nasonkini <@t> mail.nih.gov Tue May 6 18:24:14 2008 From: nasonkini <@t> mail.nih.gov (Igor Nasonkin) Date: Tue May 6 18:24:25 2008 Subject: [Histonet] Looking for detailed protocol to stain for PAS in liver sections (paraffin) Message-ID: Hi everybody, I am looking for a detailed protocol for PAS staining in liver sections (paraffin-embedded). The 1st set of sections did not work, and we had no +control since we have not done it. These are 4-6wk mouse liver sections embedded in paraffin; our lab is experienced in IHC on paraffin sections so these were done right. But PAS staining did not work. What could be main reasons? Any positive control we could use? Thank you in advance, Igor From AnthonyH <@t> chw.edu.au Tue May 6 18:31:59 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue May 6 18:32:10 2008 Subject: [Histonet] Looking for detailed protocol to stain for PAS in liver sections (paraffin) In-Reply-To: Message-ID: Use a section of appendix or other GIT tract. Mucin in the goblet cells should stain. Also look at your liver sections. Are basement membranes staining? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Igor Nasonkin Sent: Wednesday, 7 May 2008 9:24 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Looking for detailed protocol to stain for PAS in liver sections (paraffin) Hi everybody, I am looking for a detailed protocol for PAS staining in liver sections (paraffin-embedded). The 1st set of sections did not work, and we had no +control since we have not done it. These are 4-6wk mouse liver sections embedded in paraffin; our lab is experienced in IHC on paraffin sections so these were done right. But PAS staining did not work. What could be main reasons? Any positive control we could use? Thank you in advance, Igor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From gayle.callis <@t> bresnan.net Tue May 6 18:49:15 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Tue May 6 18:49:17 2008 Subject: [Histonet] RINSING IN TAP WATER AFTER DECAL References: <000001c8af9f$d3cfa200$0301a8c0@RENAD4YK9B8ABE> Message-ID: <005001c8afd3$cb12b420$6501a8c0@DHXTS541> We rinse according to size of the bone, A bone biopsy will not take long, maybe 15 minutes or so, larger bones anywhere from 30 min to a few hours. Long rinsing can swell tissues (overnight). RDO contains a high HCl concentration. One of bad things about not rinsing is the first stations in processing cycle are contaminated with decalcifier, altering pH. If you run a huge number of RDO decalcified samples, this could be a problem. HCL + formalin will form a toxic, carcingenic compound (Carson, JOH publication). I know people who simply rinsed a needle biopsy off with tap water and some don't bother, just put in processor without problems. CAL-X is a formic acid solution and is compatible with formalin. I am a proponent of rinsing and do so for all decalcifying solutions based on size of bone. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Rena Fail" To: "'Fischer, R. B'" ; Sent: Tuesday, May 06, 2008 11:37 AM Subject: RE: [Histonet] RINSING IN TAP WATER AFTER DECAL > The rinse step is necessary between formalin and RDO, the two chemicals > should not be combined > Rena > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fischer, > R. B > Sent: Tuesday, May 06, 2008 12:22 PM > To: histonet@lists.utsouthwestern.edu > Cc: Delcambre, Linda V > Subject: [Histonet] RINSING IN TAP WATER AFTER DECAL > > We have been using "RDO" and "CAL-X" decal solutions for some time now. > After the specified times in the decal solutions, the specimens are > rinsed in running tap water for about 10 minutes before being placed > back in 10%NBF for routine processing. We were rinsing to get rid of the > nitric acid. My questions are (1) Is this rinse step necessary? If it > is, (2) How long is an adequate time for rinsing?(3) How are other labs > handling the specimen post decal, and before processing? > > > > R.Brian Fischer > Histology Lead Tech > Community Hospital of the Monterey Peninsula > PO Box HH Monterey Ca. 93942 > 831-625-4791 > Fax: 831-6583683 From akemiat3377 <@t> yahoo.com Tue May 6 18:49:38 2008 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Tue May 6 18:49:41 2008 Subject: [Histonet] RINSING IN TAP WATER AFTER DECAL Message-ID: <437838.39439.qm@web31308.mail.mud.yahoo.com> Hi All, I am going to put an additional tidbit into the mix. I was instructed many many moons ago, (back in 1974) to 1st rinse off any residual RDO or decal solution with a brief rinse in tap water and then place the decal specimens into saturated sodium bicarbonate for 10 minutes to stop the decal process, otherwise the specimen will continue to decal. The tissue fizzes a bit, when this reaction ceases, then rinse in running tap water for an additional 10 minutes. Akemi Allison-Tacha, BS, HT(ASCP)HTL Client Services Manager PhenoPath laboratories 551 North 34th Street, Suite 100 Seattle, WA 98103-8675 Work: (206) 374-9000 ext 1053 E-Mail: akemiat3377@yahoo.com --- On Tue, 5/6/08, Rena Fail wrote: > From: Rena Fail > Subject: RE: [Histonet] RINSING IN TAP WATER AFTER DECAL > To: "'Fischer, R. B'" , histonet@lists.utsouthwestern.edu > Date: Tuesday, May 6, 2008, 10:37 AM > The rinse step is necessary between formalin and RDO, the > two chemicals > should not be combined > Rena > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On > Behalf Of Fischer, > R. B > Sent: Tuesday, May 06, 2008 12:22 PM > To: histonet@lists.utsouthwestern.edu > Cc: Delcambre, Linda V > Subject: [Histonet] RINSING IN TAP WATER AFTER DECAL > > We have been using "RDO" and "CAL-X" > decal solutions for some time now. > After the specified times in the decal solutions, the > specimens are > rinsed in running tap water for about 10 minutes before > being placed > back in 10%NBF for routine processing. We were rinsing to > get rid of the > nitric acid. My questions are (1) Is this rinse step > necessary? If it > is, (2) How long is an adequate time for rinsing?(3) How > are other labs > handling the specimen post decal, and before processing? > > > > R.Brian Fischer > Histology Lead Tech > Community Hospital of the Monterey Peninsula > PO Box HH Monterey Ca. 93942 > 831-625-4791 > Fax: 831-6583683 > > Confidentiality Notice: > This is a transmission from Community Hospital of the > Monterey > Peninsula. This message and any attached documents may be > confidential > and contain information protected by state and federal > medical privacy > statutes. They are intended only for the use of the > addressee. If you > are not the intended recipient, any disclosure, copying, or > distribution > of this information is strictly prohibited. If you > received this > transmission in error, please accept our apologies and > notify the > sender. > > Thank you. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Tue May 6 19:08:19 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Tue May 6 19:08:20 2008 Subject: [Histonet] Looking for detailed protocol to stain for PAS in liver sections (paraffin) References: Message-ID: <009401c8afd6$74e33a90$6501a8c0@DHXTS541> You did not say what you are trying to see in the liver? Glycogen? Some other PAS positive tissue component? If so,aqueous formalin fixation will remove the glyocgen. An alcoholic fixative to help retain glycogen. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Igor Nasonkin" To: Sent: Tuesday, May 06, 2008 5:24 PM Subject: [Histonet] Looking for detailed protocol to stain for PAS in liver sections (paraffin) > Hi everybody, > > I am looking for a detailed protocol for PAS staining in liver sections > (paraffin-embedded). The 1st set of sections did not work, and we had no > +control since we have not done it. These are 4-6wk mouse liver sections > embedded in paraffin; our lab is experienced in IHC on paraffin sections > so > these were done right. But PAS staining did not work. What could be main > reasons? Any positive control we could use? Thank you in advance, > Igor > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Tue May 6 19:39:59 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue May 6 19:39:50 2008 Subject: [Histonet] B-plus question References: Message-ID: <001001c8afda$e1985f90$0302a8c0@yourxhtr8hvc4p> Karen, we haven't had any problems and we've been using it for a couple of years. Immunos are 10 times better too. JTT ----- Original Message ----- From: "karen adams" To: Sent: Tuesday, May 06, 2008 8:00 AM Subject: [Histonet] B-plus question > Hello all....we are changing bone marrow fixative from formalin to B-plus. > If we fix for the required time in B-plu and then process on the VIP w/ > the > other specimens using formalin are there any effects on the tissue going > from B-plus to formalin?? > Thank you in advance > -- > Karen Adams > Supervisor > Pathology Laboratories West > 9303 Park West Blvd. > Knoxville, TN 37923 > kadamsplw@gmail.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jordan_2585 <@t> yahoo.com Tue May 6 21:31:52 2008 From: jordan_2585 <@t> yahoo.com (Jordan Phillips) Date: Tue May 6 21:31:55 2008 Subject: [Histonet] FISH probes Message-ID: <999031.60307.qm@web34605.mail.mud.yahoo.com> My lab is in the process of begining FISH testing.? Can anyone help with what probes to order?? Thanks ____________________________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ From jkiernan <@t> uwo.ca Wed May 7 00:11:47 2008 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed May 7 00:15:16 2008 Subject: [Histonet] Proper Grossing Attire In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3816@IS-E2K3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838C017A3816@IS-E2K3.grhs.net> Message-ID: With respects, and hoping that nobody will take offence:
Why histotechnology forum?< Kiernan
Anatomy, UWO
London, = =
----- Original Message -----
Pence <mpence@grhs.net>
Date: Tu May 6, 2008 10:40
Subject: [Histonet] Proper G rossing Attire
To: histonet@lists.utsouthwestern.edu
> is for
> "grossing in" specimens.  Two levels of grossing in are: 1)
> a punch bx
> advanced t colons-placentas.
>  < question is: "Is gloves alone appropriate attire fo
> first level?
> What do you wear w hen grossing at the second level?
>  
& save yourself much
sure.
>   advance,
> Mike
& ______________________ _______________________ 5F Histonet@lis http://lists.utsouthwester From histology.bc <@t> shaw.ca Wed May 7 01:40:53 2008 From: histology.bc <@t> shaw.ca (Paul Bradbury) Date: Wed May 7 01:34:15 2008 Subject: [Histonet] Re; RINSING IN TAP WATER AFTER DECAL In-Reply-To: <48210F0F.2080906@shaw.ca> References: <437838.39439.qm@web31308.mail.mud.yahoo.com> <48210F0F.2080906@shaw.ca> Message-ID: <48214EF5.7070602@shaw.ca> With all due respect to Akemi, the practice of "neutralizing" the decalcifying acid by treating the tissue with saturated sodium bicarbonate is not a good idea. In her e-mail she states that "The tissue fizzes a bit ..." no surprise there. Trying to retain good morphology in bone biopsy core is difficult enough without disrupting the tissues with bubbles of carbon dioxide. A bone core is usually only 2-3 mm in diameter, so washing out the decalcifying fluid will take only a few minutes. Once the acid has been removed, decalcification will stop. Paul Bradbury Kamloops, Canada ****************************************************************** > Akemi Allison-Tacha wrote: >> Hi All, >> >> I am going to put an additional tidbit into the mix. I was instructed many many moons ago, (back in 1974) to 1st rinse off any residual RDO or decal solution with a brief rinse in tap water and then place the decal specimens into saturated sodium bicarbonate for 10 minutes to stop the decal process, otherwise the specimen will continue to decal. The tissue fizzes a bit, when this reaction ceases, then rinse in running tap water for an additional 10 minutes. >> >> Akemi Allison-Tacha, BS, HT(ASCP)HTL >> Client Services Manager >> PhenoPath laboratories >> 551 North 34th Street, Suite 100 >> Seattle, WA 98103-8675 >> Work: (206) 374-9000 ext 1053 >> E-Mail: akemiat3377@yahoo.com >> >> From jennifer.l.hofecker <@t> Vanderbilt.Edu Wed May 7 07:39:21 2008 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Wed May 7 07:39:28 2008 Subject: [Histonet] TN meeting: hotel deadline Message-ID: <898D946569A27444B65667A49C0740520175B3DC@mailbe06.mc.vanderbilt.edu> Hello out there in histoland... I just wanted to take a moment to remind you of the deadline for reserving hotel rooms for the TSH meeting in June. The deadline is May 19th. We will release unsold rooms on that date. If you need more information, please visit www.nsh.org . The entire program is posted on the meetings page. You may also contact me with questions. I hope to see many of you in Townsend on June 19th. Have a great rest of the week! Jennifer L. Hofecker HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph 615.343.0083 fax 615.343.7089 NSH Quality Control Committee Chairperson TSH Secretary TSH 2008 Exhibit Coordinator From Jackie.O'Connor <@t> abbott.com Wed May 7 07:39:16 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed May 7 07:39:36 2008 Subject: [Histonet] GLP question In-Reply-To: <3AD0BD3142459B4E9B12CBEAFF2B89B2070C2617@lajamrexm01.amer.pfizer.com> Message-ID: My lab has the capability of producing 600+ slides per day. When we cut initial slides, the microtomy is documented by the microtomist at the time. Staining is subsequently documented - since it may be performed by another technician. Once documentation of a procedural step is made, that slide is already raw datum. If a recut is needed at QC, the reason for the recut is also documented, and the original slide is retained. The recut is subsequently documented, as well as whether or not the desired goal (missing mammary) was achieved. Gotta love the world of GLP. We may be documenting this to death - but it works quite well. From JCollins <@t> palmbeachpath.com Wed May 7 07:59:19 2008 From: JCollins <@t> palmbeachpath.com (Judy Collins) Date: Wed May 7 07:59:30 2008 Subject: [Histonet] Proper Grossing Attire In-Reply-To: References: <661949901A768E4F9CC16D8AF8F2838C017A3816@IS-E2K3.grhs.net> Message-ID: <05CAE76AB5D5ED409864C6DD86F1334901C95F0901@pbpsflexch02.pbp.local> His His question was not a hygiene question, but a safety compliance issue. Judy Collins -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Kiernan Sent: Wednesday, May 07, 2008 1:12 AM To: Mike Pence Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Proper Grossing Attire With respects, and hoping that nobody will take offence:
Why =e you asking a question about hygiene in a histotechnology forum?<=> 
John Kiernan
Anatomy, UWO
London,?nada.
= = =
----- Original Message -----
=om: Mike Pence <mpence@grhs.net>
Date: Tu=day, May 6, 2008 10:40
Subject: [Histonet] Proper G rossing Attire
To: histonet@lists.utsouthwestern.edu> I would like to hear everyone's opinions on what t= proper PPE
> is for
> "grossing in" specimens.  Two levels of grossing in are: 1)
>=B dictatingand submit specimen - limited to bisecting a punch bx > describing, cutting,=king selected sections - some judgment and
> advanced t=ining required ie. colons-placentas.
>  <=>> My question is: "Is gloves alone appropriate attire fo=he
> first level?
> What do you wear w hen grossing at the second level?
>  
&=; You may choose to reply off line as to save yourself much
=6gt; "flaming" I
> sure.
>  =B
> Thanks in advance,
> Mike
&=; ______________________ _______________________ 5F=F
> Histonet mailing list
> Histonet@lis=.utsouthwestern.edu
> http://lists.utsouthwester=2Eedu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed May 7 08:33:45 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 7 08:33:50 2008 Subject: [Histonet] Looking for detailed protocol to stain for PAS in liver sections (paraffin) In-Reply-To: Message-ID: <949191.32708.qm@web65703.mail.ac4.yahoo.com> Igor: PAS is an old and well known HC technique and you can find it in any histotechniques book, just follow it. The Schiff's reagent can either be bought or made but if you decide to make it yourself you should be aware that it is somewhat tricky, so my advise would be to buy it made (and keep it in the refrigerator). If you are using an already made and perhaps old solution, perhaps it did not work because it was inactive. Try it with formalin: add a few drops of the Schiff's reagent to formalin and it should instantly develop a dark magenta color and if it does not, your key reagent is bad and this could be the cause of the failure you experimented. Ren? J. Igor Nasonkin wrote: Hi everybody, I am looking for a detailed protocol for PAS staining in liver sections (paraffin-embedded). The 1st set of sections did not work, and we had no +control since we have not done it. These are 4-6wk mouse liver sections embedded in paraffin; our lab is experienced in IHC on paraffin sections so these were done right. But PAS staining did not work. What could be main reasons? Any positive control we could use? Thank you in advance, Igor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From rjbuesa <@t> yahoo.com Wed May 7 08:36:45 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 7 08:36:57 2008 Subject: [Histonet] FISH probes In-Reply-To: <999031.60307.qm@web34605.mail.mud.yahoo.com> Message-ID: <67599.39734.qm@web65708.mail.ac4.yahoo.com> Check Abbott's catalog. Ren? J. Jordan Phillips wrote: My lab is in the process of begining FISH testing. Can anyone help with what probes to order? Thanks ____________________________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From GregTesdall <@t> catholichealth.net Wed May 7 08:49:31 2008 From: GregTesdall <@t> catholichealth.net (Tesdall, Greg) Date: Wed May 7 08:50:33 2008 Subject: [Histonet] independent contractor Message-ID: Greetings. I would like to help a local hospital here in the midwest with some Histology coverage. I want to do this as an independent contractor/locums. I would appreciate input from anyone who has done this or anyone who has hired one. I need information concerning contracts, basis to charge and what to charge. Thank you. From mcauliff <@t> umdnj.edu Wed May 7 08:53:16 2008 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed May 7 08:53:10 2008 Subject: [Histonet] Looking for detailed protocol to stain for PAS in liver sections (paraffin) In-Reply-To: References: Message-ID: <4821B44C.6090605@umdnj.edu> As a number of listers have mentioned, this is not a difficult technique. That said, I always make my periodic acid fresh on the day of use. If it has been sitting around, no oxidation and no reaction. Geoff Igor Nasonkin wrote: > Hi everybody, > > I am looking for a detailed protocol for PAS staining in liver sections > (paraffin-embedded). The 1st set of sections did not work, and we had no > +control since we have not done it. These are 4-6wk mouse liver sections > embedded in paraffin; our lab is experienced in IHC on paraffin sections so > these were done right. But PAS staining did not work. What could be main > reasons? Any positive control we could use? Thank you in advance, > Igor > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From mpence <@t> grhs.net Wed May 7 09:06:56 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Wed May 7 09:07:09 2008 Subject: [Histonet] Proper Grossing Attire In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3819@IS-E2K3.grhs.net> Because I wanted to ask my fellow histologist and PA's what level of protection they are using for the level of services being provided. Mike -----Original Message----- From: John Kiernan [mailto:jkiernan@uwo.ca] Sent: Wednesday, May 07, 2008 12:12 AM To: Mike Pence Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Proper Grossing Attire With respects, and hoping that nobody will take offence: Why are you asking a question about hygiene in a histotechnology forum? John Kiernan Anatomy, UWO London, Canada. = = = ----- Original Message ----- From: Mike Pence Date: Tuesday, May 6, 2008 10:40 Subject: [Histonet] Proper Grossing Attire To: histonet@lists.utsouthwestern.edu > I would like to hear everyone's opinions on what the proper PPE > is for > "grossing in" specimens. Two levels of grossing in are: 1) > dictatingand submit specimen - limited to bisecting a punch bx > and 2) dictating, > describing, cutting, taking selected sections - some judgment and > advanced training required ie. colons-placentas. > > My question is: "Is gloves alone appropriate attire for the > first level? > What do you wear when grossing at the second level? > > You may choose to reply off line as to save yourself much > "flaming" I > sure. > > Thanks in advance, > Mike > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From michelle.schwab-macdonald <@t> novartis.com Wed May 7 10:24:30 2008 From: michelle.schwab-macdonald <@t> novartis.com (michelle.schwab-macdonald@novartis.com) Date: Wed May 7 10:24:39 2008 Subject: [Histonet] gross-section In-Reply-To: <397885.31237.qm@web33101.mail.mud.yahoo.com> Message-ID: Some manuals that I would recommend are: Surgical Pathology Dissection: An Illustrated Guide ISBN-13: 978-0387955599 Manual of Surgical Pathology ISBN-13: 978-0443079184 http://www.item.fraunhofer.de/reni/trimming/index.php - used as a guideline by many for all animal trimming (even though it only says mice and rats on the title, it is excellent) I have seen these "books" used to train many people. Let me know if you want more suggestions. I think I have a list at home. :-) Michelle Galina Deyneko Sent by: histonet-bounces@lists.utsouthwestern.edu 05/06/2008 12:29 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] gross-section Dear Colleagues Could you please advise the sources like manuals, textbooks or websites for gross-sectioning/ sampling of the organs of big mammalians ( we work with rabbits, monkeys). I think human gross-sectioning information also useful. Thank you. galina deyneko novartis,cambridge, ma --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Wed May 7 10:28:11 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Wed May 7 10:28:21 2008 Subject: [Histonet] Job opening in Great Falls, Montana for those interested Message-ID: <00ad01c8b056$f5930c30$6501a8c0@DHXTS541> Dear Histonetters, A Montana job opening for those interested in the Big Sky state. ----- Original Message ----- From: Kailey Hutchison To: gayle.callis@bresnan.net Sent: Wednesday, May 07, 2008 9:11 AM Subject: Histology Tech-Montana Good Afternoon! I am currently representing a hospital in Great Falls, Montana that is looking for a histology technician. The position is full-time, permanent, competitive pay, full benefits, and relocation assistance is offered! Please respond if you'd like to hear more! Questions: I want a permanent or contract job: I want to work in these states: I want to hear about these specific jobs: I am available to start a new job: I am looking for a salary or hourly rate of: Thanks for taking the time to fill this out and get back to me. I am looking forward to catching up with you all and helping you find your next great assignment. Have a good week ! Job Title: Histotechnician # of Positions: 1 Job Location: Montana Job Duration: full time employee Relocation Assistance: On a case by case basis JOB SUMMARY Performs all duties required for histologic examination of tissues. Also performs all duties of the Cyto-Histo Assistant. Embeds tissue samples, operates tissue processor, cuts and stains frozen section specimens, performs routine microtomy and performs special stains as requested. KNOWLEDGE/EXPERIENCE One year experience in histology required. Must be able to cut tissue using a microtome and stain tissue. Must demonstrate familiarity with anatomy, physiology, medical terminology and computer operations. Previous hospital lab experience a plus. EDUCATION Minimum High School Diploma. Prefer college course work in anatomy, physiology, medical terminology. LICENSE/CERTIFICATION/REGISTRY None required, but histotechnician certification preferred. APTITUDES Must be able to pay close attention to microscopic detail. Must demonstrate sufficient dexterity to cut very fine tissue. Must be flexible and organized to e able to perform special stains as requested. Demonstration of ability to perform duties with exceptional accuracy and efficiency. Demonstrated ability to plan and use time wisely. Ability to cope with a high work volume, requiring rapid production of accurate and satisfactory specimens for microscopic evaluation. Ability to work closely with others and with flexibility. Ability to represent the laboratory and hospital in a positive and professional manner Kailey Hutchison Senior Healthcare and HIS Recruiter Digital Prospectors Corporation 603-627-5020 x224 603-627-5025 603-361-4192-cell khutchison@dpcit.com Screen Name (AIM): KaileyBeth www.dpcit.com Link me in!! http://www.linkedin.com/in/kaileyhutchison From petunia_c <@t> hotmail.com Wed May 7 10:34:57 2008 From: petunia_c <@t> hotmail.com (barbara carter) Date: Wed May 7 10:35:01 2008 Subject: [Histonet] Histology Coordinator Position in Kenosha Wisconsin Message-ID: I am leaving my position as Coordinator at United Hospital System and am moving out of state and trying to help them recruit a new coordinator to take my place. There is a position open at United Hospital System at the Kenosha Campus in Kenosha Wisconsin for a Histology Coordinator. The hospital receives about 10,000 surgical specimen a year with 3 Pathologists and 3 Histologist. Anyone interested please contact Sue Wergin Laboratory Supervisor at 262-656-5603. _________________________________________________________________ Windows Live SkyDrive lets you share files with faraway friends. http://www.windowslive.com/skydrive/overview.html?ocid=TXT_TAGLM_WL_Refresh_skydrive_052008 From douglas-fredericks <@t> uiowa.edu Wed May 7 11:02:35 2008 From: douglas-fredericks <@t> uiowa.edu (Fredericks, Douglas) Date: Wed May 7 11:03:04 2008 Subject: [Histonet] Fluorescein labeling protocol for sheep Message-ID: <825A13A34899AB40A5039B9E1B71939F0355925B@HC-MAIL12.healthcare.uiowa.edu> I would like to know it any of you have fluorescein labeled sheep and what protocol is good to follow? I currently use this one, but I do not know if it is the best. 1. Oxytetracycline (30mg/kg IV) at xx weeks 2. Alizarin Red S (30mg/kg IV) at xx weeks 3. Fluorescein DCAF (20mg/kg IV) at xx weeks 4. Xylenol orange (90mg/kg IV) at xx weeks I also would like to know the proper protocol to make these labels. Suggestions please... Thanks, Doug ------------------------------------------ Douglas C. Fredericks Director Bone Healing Research Lab Iowa Spine Research Lab Department of Orthopaedic Surgery and Rehabilitation University of Iowa Carver College of Medicine 100 Oakdale Campus #259 ORB Iowa City, IA 52242 (319) 335-4377 (319) 335-4378 Fax douglas-fredericks@uiowa.edu From gayle.callis <@t> bresnan.net Wed May 7 11:05:39 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Wed May 7 11:05:45 2008 Subject: Fw: More on [Histonet] Looking for detailed protocol to stain for PAS in liver sections (paraffin) Message-ID: <00dc01c8b05c$330bcbb0$6501a8c0@DHXTS541> Dr. Nasonkin was staining for glycogen (per my question) and via private emailing, it was suggested he not use aqueous formalin or PFA fixation, but an alcoholic fixative to retain the glycogen in the liver, do diastase digestion to prove it is glyogen, and also process tissues - starting in higher (100%) alcohols to help retain glycogen. Suggested fixatives were Carnoy, Gendre and alcoholic formalin. If you have any other suggestions for him, please add to the list of to do's . Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT > ----- Original Message ----- > From: "Igor Nasonkin" > To: "Gayle Callis" > Cc: > Sent: Tuesday, May 06, 2008 6:35 PM > Subject: Re: [Histonet] Looking for detailed protocol to stain for PAS in > liver sections (paraffin) > > > Gayle, > Yes, glycogen. > Does it mean that if we fixed livers in paraformaldehyde prepared on PBS > we > lost glycogen? What if we fixed the whole liver, not section on a slide? > One > cannot remove glycogen from the whole liver this way. Thank you for this > info, > Igor > > Dr. Igor O. Nasonkin > Research Fellow > National Institutes of Health/NEI > 9000 Rockville Pike, MSC 1864 > Bldg 10, Room 10B11 > Bethesda, MD 20892 > Tel: 301-443-7398 ?work > 617-388-4104 ?cell > Fax 301-480-1769 > email: nasonkini@mail.nih.gov > http://www.nei.nih.gov/intramural/nnrl.asp > > On 5/6/08 8:08 PM, "Gayle Callis" wrote: > >> You did not say what you are trying to see in the liver? Glycogen? Some >> other PAS positive tissue component? If so,aqueous formalin fixation >> will remove the glyocgen. An alcoholic fixative helps retain glycogen. >> >> Gayle M. Callis >> HTL/HT/MT(ASCP) >> Bozeman MT 59715 >> >> ----- Original Message ----- >> From: "Igor Nasonkin" >> To: >> Sent: Tuesday, May 06, 2008 5:24 PM >> Subject: [Histonet] Looking for detailed protocol to stain for PAS in >> liver >> sections (paraffin) >> >>> I am looking for a detailed protocol for PAS staining in liver sections >>> (paraffin-embedded). The 1st set of sections did not work, and we had no >>> +control since we have not done it. These are 4-6wk mouse liver sections >>> embedded in paraffin; our lab is experienced in IHC on paraffin sections >>> so >>> these were done right. But PAS staining did not work. What could be main >>> reasons? Any positive control we could use? Thank you in advance, >>> Igor From annigyg <@t> gmail.com Wed May 7 11:09:14 2008 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Wed May 7 11:09:19 2008 Subject: [Histonet] Histology Coordinator Position in Kenosha Wisconsin In-Reply-To: References: Message-ID: i have a few questions for you if i may. you have 3 techs serving 3 paths and 10 000 a year apart from the basic routine histo operations, do your 3 techs - do any grossing - attend at renal biopsy collection - do frozen sections - take macro photos - do slide and block filing - source cases for sendout (consults/reviews) or do any archive searches - wash glassware, - do specimen discards, - make up formalin i am facing pressure from my (non-histo) boss who is comparing my lab and staff to 'other modern facilities' and he now tells me i need to cut my staff in half!! i need ammo and numbers from others to build my defence cheers Annie 2008/5/7 barbara carter : > > I am leaving my position as Coordinator at United Hospital System and am > moving out of state and trying to help them recruit a new coordinator to > take my place. > > There is a position open at United Hospital System at the Kenosha Campus > in Kenosha Wisconsin for a Histology Coordinator. The hospital receives > about 10,000 surgical specimen a year with 3 Pathologists and 3 Histologist. > > Anyone interested please contact Sue Wergin Laboratory Supervisor at > 262-656-5603. > > > > > _________________________________________________________________ > Windows Live SkyDrive lets you share files with faraway friends. > > http://www.windowslive.com/skydrive/overview.html?ocid=TXT_TAGLM_WL_Refresh_skydrive_052008_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne (van Binsbergen) Hope Abu Dhabi UAE From rjbuesa <@t> yahoo.com Wed May 7 11:15:11 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 7 11:15:18 2008 Subject: [Histonet] Proper Grossing Attire In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3819@IS-E2K3.grhs.net> Message-ID: <418172.33040.qm@web65710.mail.ac4.yahoo.com> Mike: In our lab the residents (those in charge of grossing) used the following: gloves, impervious aprons, and face shield/goggles. When dealing with bones, they also used cut resistant gloves. Feet covers were also worn. Ren? J. Mike Pence wrote: --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From Jessica.Vacca <@t> HCAhealthcare.com Wed May 7 11:34:37 2008 From: Jessica.Vacca <@t> HCAhealthcare.com (Vacca Jessica) Date: Wed May 7 11:34:46 2008 Subject: [Histonet] Autopsy P&P Message-ID: <41E16A15CE78374EA45B57E0F94339B803BABA15@ORLEV01.hca.corpad.net> Does anyone have an autopsy P&P that they would like to share that has basically the following criteria? Risk management approval Physician requested (not family) Outside facility performs. Granted it does not have to meet all, but I'm needing somewhere to begin. Thanks in advance for help. Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr. Brandon Fl 33511 (813) 571-5193 or (813) 681-5551 ext 2454 Jessica.Vacca@hcahealthcare.com From Janet.Bonner <@t> FLHOSP.ORG Wed May 7 12:06:17 2008 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Wed May 7 12:06:31 2008 Subject: [Histonet] Too many Techs... References: Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2621@fhosxchmb006.ADVENTISTCORP.NET> 10,000cases? 3300 cases per tech per year, 100 cases per tech per day? At least 300 blocks min. per day per tech. CAP has guidelines for justifying positions. I would also have the techs (like they need something else to do) keep track of their time for a month. It sounds as if three techs would minimally handle your lab. Not to mention two months worth of vacation/sick time taken per year. Also - what is the boss seeing? Coffee breaks? Long lunch breaks? We have 22 Techs, three shifts, six days per week, 50,000 Surgical cases. It takes two techs worth just to cover time off per year. We do not attend procedures and we buy our reagents. Good luck. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Anne van Binsbergen Sent: Wed 5/7/2008 12:09 PM To: barbara carter; histonet-request@lists.utsouthwestern.edu; Histonet Subject: Re: [Histonet] Histology Coordinator Position in Kenosha Wisconsin i have a few questions for you if i may. you have 3 techs serving 3 paths and 10 000 a year apart from the basic routine histo operations, do your 3 techs - do any grossing - attend at renal biopsy collection - do frozen sections - take macro photos - do slide and block filing - source cases for sendout (consults/reviews) or do any archive searches - wash glassware, - do specimen discards, - make up formalin i am facing pressure from my (non-histo) boss who is comparing my lab and staff to 'other modern facilities' and he now tells me i need to cut my staff in half!! i need ammo and numbers from others to build my defence cheers Annie 2008/5/7 barbara carter : > > I am leaving my position as Coordinator at United Hospital System and am > moving out of state and trying to help them recruit a new coordinator to > take my place. > > There is a position open at United Hospital System at the Kenosha Campus > in Kenosha Wisconsin for a Histology Coordinator. The hospital receives > about 10,000 surgical specimen a year with 3 Pathologists and 3 Histologist. > > Anyone interested please contact Sue Wergin Laboratory Supervisor at > 262-656-5603. > > > > > _________________________________________________________________ > Windows Live SkyDrive lets you share files with faraway friends. > > http://www.windowslive.com/skydrive/overview.html?ocid=TXT_TAGLM_WL_Refresh_skydrive_052008_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne (van Binsbergen) Hope Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From Susan.Weber2 <@t> va.gov Wed May 7 12:09:45 2008 From: Susan.Weber2 <@t> va.gov (Weber, Susan (VHACLE)) Date: Wed May 7 12:09:51 2008 Subject: [Histonet] B-plus question In-Reply-To: <482062A0.4080500@shaw.ca> References: <482062A0.4080500@shaw.ca> Message-ID: <16C83872A53F4346AA9C3A18E3A3AAB903F76D96@VHAV10MSGA1.v10.med.va.gov> I may have missed this, but may I have the name of the Vendor you purchase your B-plus from. Thanks. Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paul Bradbury Sent: Tuesday, May 06, 2008 9:53 AM To: karen adams; HistoNet Server Subject: Re: [Histonet] B-plus question Hi Karen, I have been using the same sequence of reagents for several years with great success. We routinely fix bone marrow cores for 3 hours in B-plus, rinse in water, decalcify, rinse again, and put the cassette in with all the other tissues for processing. B-plus contains formaldehyde anyway, so your are not introducing a different reagent when you transfer them to formalin during processing. B-plus gives much better cytological detail than formalin. Nuclear detail is crisper, granules are better preserved, hemosiderin is not effected. CD-3, CD-20, kappa and lambda, etc. all work beautifully after B-plus. It also has the advantage of not producing an fixation artefact pigment like B-5 does. I think you will be happy with your decision to change. Paul Bradbury Kamloops, Canada karen adams wrote: > Hello all....we are changing bone marrow fixative from formalin to B-plus. > If we fix for the required time in B-plu and then process on the VIP w/ the > other specimens using formalin are there any effects on the tissue going > from B-plus to formalin?? > Thank you in advance > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Wed May 7 12:24:29 2008 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed May 7 12:24:32 2008 Subject: [Histonet] cryoprotection In-Reply-To: <977462.62375.qm@web62515.mail.re1.yahoo.com> References: <977462.62375.qm@web62515.mail.re1.yahoo.com> Message-ID: Transfer the brain, after adequate fixation in buffered forma brain sin cutting sections. should still freeze as q on a metal cryostat chuck, compound for adhesion, then stand the chu CO2 and either acetone or alcohol. Mount the c in the cryostat and wait for its temperature  equilibrate. This isn't the fastest way to freeze thing but it's good enough for cryoprotected formaldehyde-fixed rodent brain UWO

----- Or <tshrobertso May 5, 2008 12:41< cryoprotection
To: histonet@li sts.utsouthwestern.edu

> i am trying to cryopr
> brain in sucrose. can you please
to prevent holes in my sample?
> ---------------------------------
> Be a
> ______ _______________________ 5F _________________
> Hist Histonet@lists.utsouthwestern.ed http://lists.utsouthwestern.edu/mailman/listinfo From Dorothy.L.Webb <@t> HealthPartners.Com Wed May 7 12:56:24 2008 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Wed May 7 12:56:30 2008 Subject: [Histonet] Lot numbers Message-ID: <0E394B648E5284478A6CCB78E5AFDA27056356BB@hpes1.HealthPartners.int> Does everyone keep track of each reagent lot number, including, water, formalin, alcohols, etc. as it is opened and used? What about recycled products? I am trying to come up with a logsheet and would appreciate any help in this area! Also, we have recently run into problems with our freezing compound coming off of the chucks during frozen sectioning. Any suggestions and/or what is the method of freezing tissue that everyone uses for their cryostat sections? Thanks fellow histotechs for helping me with these matters that I am coming up empty on!!!!!!!!!!!!!!!!!!!!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From michelle.schwab-macdonald <@t> novartis.com Wed May 7 12:56:46 2008 From: michelle.schwab-macdonald <@t> novartis.com (michelle.schwab-macdonald@novartis.com) Date: Wed May 7 12:56:59 2008 Subject: [Histonet] c-kit (CD117) rat and mouse tissue In-Reply-To: <005001c8afd3$cb12b420$6501a8c0@DHXTS541> Message-ID: I am looking for a c-kit Ab that will stain FFPE rat or mouse tissue. I have heard that Dako has one for rat. Is anyone using it and willing to share protocol information or does anyone have an Ab suggestion? Thanks in Advance!! :-) Michelle Schwab-MacDonald HT(ASCP) Novartis, Boston, MA From Shirley_PHUA <@t> hsa.gov.sg Wed May 7 13:02:51 2008 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Wed May 7 13:05:06 2008 Subject: [Histonet] Shirley Phua is out-of-office ... Message-ID: I will be out of the office from 08-05-2008 to 09-05-2008. I'll be away on 08 May 2008. Pathologists: I will process your requests when I return. If urgent, please forward your email to Henry_Kyaw@hsa.gov.sg From dusko.trajkovic <@t> pfizer.com Wed May 7 13:16:21 2008 From: dusko.trajkovic <@t> pfizer.com (Trajkovic, Dusko) Date: Wed May 7 13:16:45 2008 Subject: [Histonet] GLP question In-Reply-To: Message-ID: <3AD0BD3142459B4E9B12CBEAFF2B89B2070C2917@lajamrexm01.amer.pfizer.com> If our lab had the same operation as Jackie O., then that is the way I would also perform the GLP tracking. However, our set up is similar to Kim Merriam, where each Histotech is responsible for their own study or work assigned. One person will do the trimming, embedding, sectioning, staining and self QC. I don't consider my slides as final product until I sign off on the QC portion, have someone else verify and initial that my numbers are correct (blocks and slides match and are all accounted for) then hand the slides to the pathologist. During my QC process, if I find that there are imperfections in the sections (this is hypothetical, since things like that never happen to me) such as folds, knife marks, floaters, etc. I go back to recut the slide, restain and QC again. If I am satisfied with my new freshly coverslipped slide, I sign off the animal and submit my slides. The slide that I discarded is nothing more than any other section that I discarded during my microtome sectioning before deciding that I am deep enough in the block and it's time to collect my ribbon and place on the water bath. This is my story, and my opinion on the GLP process. I'm sure that someone else might have some issues with my process, but every situation is different. Having worked in a GLP lab for quite a few years, never had any issues with FDA inspectors regarding any work that I have submitted. Dusko Trajkovic Pfizer Inc, La Jolla ________________________________ From: Jackie M O'Connor [mailto:Jackie.O'Connor@abbott.com] Sent: Wednesday, May 07, 2008 5:39 AM To: Trajkovic, Dusko Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu; Kim Merriam; Linke_Noelle Subject: RE: [Histonet] GLP question My lab has the capability of producing 600+ slides per day. When we cut initial slides, the microtomy is documented by the microtomist at the time. Staining is subsequently documented - since it may be performed by another technician. Once documentation of a procedural step is made, that slide is already raw datum. If a recut is needed at QC, the reason for the recut is also documented, and the original slide is retained. The recut is subsequently documented, as well as whether or not the desired goal (missing mammary) was achieved. Gotta love the world of GLP. We may be documenting this to death - but it works quite well. From Charlene.Henry <@t> STJUDE.ORG Wed May 7 14:53:42 2008 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Wed May 7 14:53:49 2008 Subject: [Histonet] Lot numbers. . In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA27056356BB@hpes1.HealthPartners.int> Message-ID: <03E1F5968F60C5448635D49D38B283ED797B597D@SJMEMXMBS11.stjude.sjcrh.local> We had put the tracking of lot numbers and expiration dates of reagents (alcohol, xylene, methanol, formalin etc) into place just before our last CAP inspection and I'm glad we did because we would have been sited with a deficiency. I don't think that it is on the AP Checklist but it is on the General Checklist. The inspector asking me about the reagent lot numbers and expiration dates was a chemistry person and I tried to explain to her that the volume of reagents that a Histology Lab goes through in comparison with a Chemistry Lab is quite different. I told her that a Histology Lab would go through more reagents than the rest of all the clinical labs put together. I would think it would be impossible to track the lot number of recycled alcohols. We don't track the lot numbers of water because we have a Millipore system. We do track the lot number of formalin as it comes in and is put into use; however we do not track which lot number of formalin is used on individual cases. My experience with the compound coming off chucks during frozen sections has been when the chuck is too cold so we do not store our chucks in the cryostat but keep them at room temperature. It really does not add that much time when freezing tissue. Charlene Henry HT (ASCP), QIHC Anatomic Pathology Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, May 07, 2008 12:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Lot numbers. . Does everyone keep track of each reagent lot number, including, water, formalin, alcohols, etc. as it is opened and used? What about recycled products? I am trying to come up with a logsheet and would appreciate any help in this area! Also, we have recently run into problems with our freezing compound coming off of the chucks during frozen sectioning. Any suggestions and/or what is the method of freezing tissue that everyone uses for their cryostat sections? Thanks fellow histotechs for helping me with these matters that I am coming up empty on!!!!!!!!!!!!!!!!!!!!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charlene.Henry <@t> STJUDE.ORG Wed May 7 14:58:49 2008 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Wed May 7 14:58:57 2008 Subject: [Histonet] B-plus question. . In-Reply-To: <16C83872A53F4346AA9C3A18E3A3AAB903F76D96@VHAV10MSGA1.v10.med.va.gov> Message-ID: <03E1F5968F60C5448635D49D38B283ED797B597F@SJMEMXMBS11.stjude.sjcrh.local> We get our B-plus from BBC Biomedical. Charlene -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weber, Susan (VHACLE) Sent: Wednesday, May 07, 2008 12:10 PM To: Paul Bradbury; karen adams; HistoNet Server Subject: RE: [Histonet] B-plus question. . I may have missed this, but may I have the name of the Vendor you purchase your B-plus from. Thanks. Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paul Bradbury Sent: Tuesday, May 06, 2008 9:53 AM To: karen adams; HistoNet Server Subject: Re: [Histonet] B-plus question Hi Karen, I have been using the same sequence of reagents for several years with great success. We routinely fix bone marrow cores for 3 hours in B-plus, rinse in water, decalcify, rinse again, and put the cassette in with all the other tissues for processing. B-plus contains formaldehyde anyway, so your are not introducing a different reagent when you transfer them to formalin during processing. B-plus gives much better cytological detail than formalin. Nuclear detail is crisper, granules are better preserved, hemosiderin is not effected. CD-3, CD-20, kappa and lambda, etc. all work beautifully after B-plus. It also has the advantage of not producing an fixation artefact pigment like B-5 does. I think you will be happy with your decision to change. Paul Bradbury Kamloops, Canada karen adams wrote: > Hello all....we are changing bone marrow fixative from formalin to B-plus. > If we fix for the required time in B-plu and then process on the VIP w/ the > other specimens using formalin are there any effects on the tissue going > from B-plus to formalin?? > Thank you in advance > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Christen <@t> vet.k-state.edu Wed May 7 15:02:31 2008 From: Christen <@t> vet.k-state.edu (Shelly Christenson) Date: Wed May 7 15:03:28 2008 Subject: [Histonet] Immunohistotechnologist position open at Kansas State Univ. Message-ID: <4821C486.EF61.003F.0@vet.k-state.edu> There is a position in the Immunohistochemistry section of the Histology Lab of the Kansas State Veterinary Diagnostic Laboratory. The successful candidate will have a background in Immunohictochemeistry, Immunofluorescence, and other aspects of histology . Supervisory experience will be beneficial. A Bachelor of Science or equivalent degree and a minimum of one year of laboratory experience is required. This is just a short version of the job description for more information can contact: Dr. Brad DeBey, DVM, PhD 785/532-4481 debey@vet.k-state.edu Also it posited online https://www.da.ks.gov/ps/esummary/es-online/frmes1.asp Requisition number is 160443 Feel free to contact me also. Shelly Christenson HT(ASCP) 785/532-4464 christen@vet.k-state.edu From rjbuesa <@t> yahoo.com Wed May 7 15:03:32 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 7 15:03:36 2008 Subject: [Histonet] Lot numbers. . In-Reply-To: <03E1F5968F60C5448635D49D38B283ED797B597D@SJMEMXMBS11.stjude.sjcrh.local> Message-ID: <314908.74685.qm@web65707.mail.ac4.yahoo.com> The only lot numbers I kept track of were those of "critical reagents", namely: any one dealing with any IHC procedure and the few staining solutions bought commercially. Ren? J. "Henry, Charlene" wrote: --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From Janet.Bonner <@t> FLHOSP.ORG Wed May 7 15:08:49 2008 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Wed May 7 15:10:48 2008 Subject: [Histonet] Lot numbers References: <0E394B648E5284478A6CCB78E5AFDA27056356BB@hpes1.HealthPartners.int> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F262B@fhosxchmb006.ADVENTISTCORP.NET> We have this happen when the chuck is the temperature of the cryostat before we put the OCT on it. The OCT freezes before it has a chance to seep into the grooves. Try putting the OCT on the warm chuck just after you put the chuck on the bar in the cryostat. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Webb, Dorothy L Sent: Wed 5/7/2008 1:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Lot numbers Does everyone keep track of each reagent lot number, including, water, formalin, alcohols, etc. as it is opened and used? What about recycled products? I am trying to come up with a logsheet and would appreciate any help in this area! Also, we have recently run into problems with our freezing compound coming off of the chucks during frozen sectioning. Any suggestions and/or what is the method of freezing tissue that everyone uses for their cryostat sections? Thanks fellow histotechs for helping me with these matters that I am coming up empty on!!!!!!!!!!!!!!!!!!!!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From Clarissa.Wright <@t> med.navy.mil Wed May 7 15:13:04 2008 From: Clarissa.Wright <@t> med.navy.mil (Wright, Clarissa B CIV) Date: Wed May 7 15:13:12 2008 Subject: [Histonet] c-kit (CD117) rat and mouse tissue In-Reply-To: References: <005001c8afd3$cb12b420$6501a8c0@DHXTS541> Message-ID: <8072C1D1C2865C49B7F0A95FC47B7646AABF35@NMCSD-EX-VS-01.nmed.ds.med.navy.mil> Michelle, Try Lifespan Biosciences, they have several. I have not tried them, so I can't help you with the protocol. Kris -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of michelle.schwab-macdonald@novartis.com Sent: Wednesday, May 07, 2008 10:57 AM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] c-kit (CD117) rat and mouse tissue I am looking for a c-kit Ab that will stain FFPE rat or mouse tissue. I have heard that Dako has one for rat. Is anyone using it and willing to share protocol information or does anyone have an Ab suggestion? Thanks in Advance!! :-) Michelle Schwab-MacDonald HT(ASCP) Novartis, Boston, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From zodiac29 <@t> comcast.net Wed May 7 16:00:51 2008 From: zodiac29 <@t> comcast.net (zodiac29@comcast.net) Date: Wed May 7 16:00:56 2008 Subject: [Histonet] histonet@lists.utsouthwestern.edu Message-ID: <050720082100.4274.48221883000BDFE3000010B22216557996C7CD0C0E070B0196@comcast.net> Hello, I have read somewhere that silver solutions should be stored in an expolsive proof refrigerator. Is this true? Right know we just store them in a regular house hold type fridge. Can anyone comment on this? Thanks, Jenny From laurie.colbert <@t> huntingtonhospital.com Wed May 7 16:11:39 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed May 7 16:11:43 2008 Subject: [Histonet] glassware cleaners Message-ID: <57BE698966D5C54EAE8612E8941D768302E38DDF@EXCHANGE3.huntingtonhospital.com> I have used a detergent called Contrad 70 for years to clean my glassware, but it is no longer available from my vendor. What are others using to clean iron solutions, muci stain, silver, etc off of their glassware? Laurie From AnthonyH <@t> chw.edu.au Wed May 7 18:17:57 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed May 7 18:18:09 2008 Subject: More on [Histonet] Looking for detailed protocol to stain for PASin liver sections (paraffin) In-Reply-To: <00dc01c8b05c$330bcbb0$6501a8c0@DHXTS541> Message-ID: I have found the following Modified PAS procedure especially useful for cytology smears that have been fixed in ethanol: Alcoholic PAS Stain Mucins and glycogen are water soluble. Over-rinsing slides, especially cytological smears, could result in excessive loss of these PAS substances. The following variant of the PAS stain performs the reactions in alcohol, thus decreasing the loss of these substances. Solutions: 1. Alcoholic Schiff reagent Basic Fuchsin 0.5g Ethanol 80ml Distilled Water 20ml Hydrochloric acid 1ml 2. Alcoholic Periodic acid Periodic acid 0.5g 95% ethanol 50ml Procedure: 1. Fix smears in 95% ethanol or use methanol fixed air-dried smears. 2. Place in alcoholic periodic acid 20min. 3. Wash in alcohol 4. Place in alcoholic Schiff's 20min. 5. Rinse slides in alcohol to remove excess dye. 6. Rinse slides in water. 7. Counterstain slides in Haematoxylin 2min. 8. Rinse slides in water. 9. Differentiate and Blue. 10. Dehydrate, clear and mount. References: Horobin and Kevill-Davis (1971) Stain Techn 46:53-58. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Thursday, 8 May 2008 2:06 AM To: Histonet Subject: Fw: More on [Histonet] Looking for detailed protocol to stain for PASin liver sections (paraffin) Dr. Nasonkin was staining for glycogen (per my question) and via private emailing, it was suggested he not use aqueous formalin or PFA fixation, but an alcoholic fixative to retain the glycogen in the liver, do diastase digestion to prove it is glyogen, and also process tissues - starting in higher (100%) alcohols to help retain glycogen. Suggested fixatives were Carnoy, Gendre and alcoholic formalin. If you have any other suggestions for him, please add to the list of to do's . Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT > ----- Original Message ----- > From: "Igor Nasonkin" > To: "Gayle Callis" > Cc: > Sent: Tuesday, May 06, 2008 6:35 PM > Subject: Re: [Histonet] Looking for detailed protocol to stain for PAS > in liver sections (paraffin) > > > Gayle, > Yes, glycogen. > Does it mean that if we fixed livers in paraformaldehyde prepared on > PBS > we > lost glycogen? What if we fixed the whole liver, not section on a slide? > One > cannot remove glycogen from the whole liver this way. Thank you for this > info, > Igor > > Dr. Igor O. Nasonkin > Research Fellow > National Institutes of Health/NEI > 9000 Rockville Pike, MSC 1864 > Bldg 10, Room 10B11 > Bethesda, MD 20892 > Tel: 301-443-7398 -work > 617-388-4104 -cell > Fax 301-480-1769 > email: nasonkini@mail.nih.gov > http://www.nei.nih.gov/intramural/nnrl.asp > > On 5/6/08 8:08 PM, "Gayle Callis" wrote: > >> You did not say what you are trying to see in the liver? Glycogen? Some >> other PAS positive tissue component? If so,aqueous formalin fixation >> will remove the glyocgen. An alcoholic fixative helps retain >> glycogen. >> >> Gayle M. Callis >> HTL/HT/MT(ASCP) >> Bozeman MT 59715 >> >> ----- Original Message ----- >> From: "Igor Nasonkin" >> To: >> Sent: Tuesday, May 06, 2008 5:24 PM >> Subject: [Histonet] Looking for detailed protocol to stain for PAS in >> liver sections (paraffin) >> >>> I am looking for a detailed protocol for PAS staining in liver >>> sections (paraffin-embedded). The 1st set of sections did not work, >>> and we had no >>> +control since we have not done it. These are 4-6wk mouse liver >>> +sections >>> embedded in paraffin; our lab is experienced in IHC on paraffin >>> sections so these were done right. But PAS staining did not work. >>> What could be main reasons? Any positive control we could use? Thank >>> you in advance, Igor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Wed May 7 18:29:21 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed May 7 18:29:27 2008 Subject: More on [Histonet] Looking for detailed protocol to stain for PASin liver sections (paraffin) In-Reply-To: <000801c8b099$649c7840$6501a8c0@DHXTS541> Message-ID: Sorry, I have tried it on methanol fixed frozen sections of liver and it works quite well Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: Gayle Callis [mailto:gayle.callis@bresnan.net] Sent: Thursday, 8 May 2008 9:24 AM To: Tony Henwood Subject: Re: More on [Histonet] Looking for detailed protocol to stain for PASin liver sections (paraffin) Tony, What a delightful variation and I bet it would work on tissue sections too. Gayle Callis ----- Original Message ----- From: "Tony Henwood" To: "Gayle Callis" ; "Histonet" Sent: Wednesday, May 07, 2008 5:17 PM Subject: RE: More on [Histonet] Looking for detailed protocol to stain for PASin liver sections (paraffin) I have found the following Modified PAS procedure especially useful for cytology smears that have been fixed in ethanol: Alcoholic PAS Stain Mucins and glycogen are water soluble. Over-rinsing slides, especially cytological smears, could result in excessive loss of these PAS substances. The following variant of the PAS stain performs the reactions in alcohol, thus decreasing the loss of these substances. Solutions: 1. Alcoholic Schiff reagent Basic Fuchsin 0.5g Ethanol 80ml Distilled Water 20ml Hydrochloric acid 1ml 2. Alcoholic Periodic acid Periodic acid 0.5g 95% ethanol 50ml Procedure: 1. Fix smears in 95% ethanol or use methanol fixed air-dried smears. 2. Place in alcoholic periodic acid 20min. 3. Wash in alcohol 4. Place in alcoholic Schiff's 20min. 5. Rinse slides in alcohol to remove excess dye. 6. Rinse slides in water. 7. Counterstain slides in Haematoxylin 2min. 8. Rinse slides in water. 9. Differentiate and Blue. 10. Dehydrate, clear and mount. References: Horobin and Kevill-Davis (1971) Stain Techn 46:53-58. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Thursday, 8 May 2008 2:06 AM To: Histonet Subject: Fw: More on [Histonet] Looking for detailed protocol to stain for PASin liver sections (paraffin) Dr. Nasonkin was staining for glycogen (per my question) and via private emailing, it was suggested he not use aqueous formalin or PFA fixation, but an alcoholic fixative to retain the glycogen in the liver, do diastase digestion to prove it is glyogen, and also process tissues - starting in higher (100%) alcohols to help retain glycogen. Suggested fixatives were Carnoy, Gendre and alcoholic formalin. If you have any other suggestions for him, please add to the list of to do's . Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT > ----- Original Message ----- > From: "Igor Nasonkin" > To: "Gayle Callis" > Cc: > Sent: Tuesday, May 06, 2008 6:35 PM > Subject: Re: [Histonet] Looking for detailed protocol to stain for PAS > in liver sections (paraffin) > > > Gayle, > Yes, glycogen. > Does it mean that if we fixed livers in paraformaldehyde prepared on > PBS we > lost glycogen? What if we fixed the whole liver, not section on a slide? > One > cannot remove glycogen from the whole liver this way. Thank you for this > info, > Igor > > Dr. Igor O. Nasonkin > Research Fellow > National Institutes of Health/NEI > 9000 Rockville Pike, MSC 1864 > Bldg 10, Room 10B11 > Bethesda, MD 20892 > Tel: 301-443-7398 -work > 617-388-4104 -cell > Fax 301-480-1769 > email: nasonkini@mail.nih.gov > http://www.nei.nih.gov/intramural/nnrl.asp > > On 5/6/08 8:08 PM, "Gayle Callis" wrote: > >> You did not say what you are trying to see in the liver? Glycogen? Some >> other PAS positive tissue component? If so,aqueous formalin fixation >> will remove the glyocgen. An alcoholic fixative helps retain >> glycogen. >> >> Gayle M. Callis >> HTL/HT/MT(ASCP) >> Bozeman MT 59715 >> >> ----- Original Message ----- >> From: "Igor Nasonkin" >> To: >> Sent: Tuesday, May 06, 2008 5:24 PM >> Subject: [Histonet] Looking for detailed protocol to stain for PAS in >> liver sections (paraffin) >> >>> I am looking for a detailed protocol for PAS staining in liver >>> sections (paraffin-embedded). The 1st set of sections did not work, >>> and we had no >>> +control since we have not done it. These are 4-6wk mouse liver >>> +sections >>> embedded in paraffin; our lab is experienced in IHC on paraffin >>> sections so these were done right. But PAS staining did not work. >>> What could be main reasons? Any positive control we could use? Thank >>> you in advance, Igor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From akemiat3377 <@t> yahoo.com Wed May 7 19:11:11 2008 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Wed May 7 19:11:15 2008 Subject: [Histonet] Re; Decal Process From the Past In-Reply-To: <48214EF5.7070602@shaw.ca> Message-ID: <251486.25591.qm@web31304.mail.mud.yahoo.com> Hi Paul, I appreciate your courteous reply to the older methodologies, but as I stated, this was just "throwing an additional tidbit into the mix". As you are most likely aware, histology in the past was not considered an exact science. Histotech's referred to formula's as recipes and the results for the most part, depended on the phase on the moon and the technical skill and knowledge of the individual. Most of the histotech's I ran a crossed in the 60's, 70's, 80's and even the 90's were OJT. There are histotech's that are still following method's that were passed on to them years and years ago and aren't sure why. This procedure was for larger bone specimens. We never put BM cores into RDO, that solution was much too harsh for such a fragile specimen. For BM cores, we used a gentle decal solution that had EDTA incorporated into it. It usually only took a half hour and we never left it longer than an hour. We rinsed the decalcified BM core in running tap water, then placed it on the processor. When I started doing technical support for a biotech company, I became aware of the "old recipes" and variations in adhering to set procedures. There were a number of histotech's that had NO idea why they were doing a procedure, or how each chemical step impacted the final result. These histotech's had no clue of what theory and practice was all about. It was a wonderful opportunity to try to help my fellow histologists in any way possible and gave me a opportunity to grow as well. A day does not go by that I don't grow and learn something new. Akemi Allison-Tacha, BS, HT(ASCP)HTL Client Services Manager PhenoPath laboratories 551 North 34th Street, Suite 100 Seattle, WA 98103-8675 Work: (206) 374-9000 ext 1053 E-Mail: akemiat3377@yahoo.com --- On Tue, 5/6/08, Paul Bradbury wrote: > From: Paul Bradbury > Subject: [Histonet] Re; RINSING IN TAP WATER AFTER DECAL > To: "HistoNet Server" > Date: Tuesday, May 6, 2008, 11:40 PM > With all due respect to Akemi, the practice of > "neutralizing" the > decalcifying acid by treating the tissue with saturated > sodium > bicarbonate is not a good idea. > In her e-mail she states that "The tissue fizzes a bit > ..." no surprise > there. Trying to retain good morphology in bone biopsy core > is difficult > enough without disrupting the tissues with bubbles of > carbon dioxide. A > bone core is usually only 2-3 mm in diameter, so washing > out the > decalcifying fluid will take only a few minutes. Once the > acid has been > removed, decalcification will stop. > Paul Bradbury > Kamloops, Canada > > ****************************************************************** > > > Akemi Allison-Tacha wrote: > >> Hi All, > >> > >> I am going to put an additional tidbit into the > mix. I was instructed many many moons ago, (back in 1974) > to 1st rinse off any residual RDO or decal solution with a > brief rinse in tap water and then place the decal specimens > into saturated sodium bicarbonate for 10 minutes to stop the > decal process, otherwise the specimen will continue to > decal. The tissue fizzes a bit, when this reaction ceases, > then rinse in running tap water for an additional 10 > minutes. > >> > >> Akemi Allison-Tacha, BS, HT(ASCP)HTL > >> Client Services Manager > >> PhenoPath laboratories > >> 551 North 34th Street, Suite 100 > >> Seattle, WA 98103-8675 > >> Work: (206) 374-9000 ext 1053 > >> E-Mail: akemiat3377@yahoo.com > >> > >> > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jstaruk <@t> masshistology.com Wed May 7 19:20:36 2008 From: jstaruk <@t> masshistology.com (jstaruk) Date: Wed May 7 19:20:52 2008 Subject: [Histonet] Too many Techs... In-Reply-To: <5F31F38C96781A4FBE3196EBC22D47807F2621@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: _____________________ Jim Staruk Mass Histology Service www.masshistology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonner, Janet Sent: Wednesday, May 07, 2008 1:06 PM To: Anne van Binsbergen; barbara carter; histonet-request@lists.utsouthwestern.edu; Histonet Subject: [Histonet] Too many Techs... 10,000cases? 3300 cases per tech per year, 100 cases per tech per day? At least 300 blocks min. per day per tech. CAP has guidelines for justifying positions. I would also have the techs (like they need something else to do) keep track of their time for a month. It sounds as if three techs would minimally handle your lab. Not to mention two months worth of vacation/sick time taken per year. Also - what is the boss seeing? Coffee breaks? Long lunch breaks? We have 22 Techs, three shifts, six days per week, 50,000 Surgical cases. It takes two techs worth just to cover time off per year. We do not attend procedures and we buy our reagents. Good luck. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Anne van Binsbergen Sent: Wed 5/7/2008 12:09 PM To: barbara carter; histonet-request@lists.utsouthwestern.edu; Histonet Subject: Re: [Histonet] Histology Coordinator Position in Kenosha Wisconsin i have a few questions for you if i may. you have 3 techs serving 3 paths and 10 000 a year apart from the basic routine histo operations, do your 3 techs - do any grossing - attend at renal biopsy collection - do frozen sections - take macro photos - do slide and block filing - source cases for sendout (consults/reviews) or do any archive searches - wash glassware, - do specimen discards, - make up formalin i am facing pressure from my (non-histo) boss who is comparing my lab and staff to 'other modern facilities' and he now tells me i need to cut my staff in half!! i need ammo and numbers from others to build my defence cheers Annie 2008/5/7 barbara carter : > > I am leaving my position as Coordinator at United Hospital System and am > moving out of state and trying to help them recruit a new coordinator to > take my place. > > There is a position open at United Hospital System at the Kenosha Campus > in Kenosha Wisconsin for a Histology Coordinator. The hospital receives > about 10,000 surgical specimen a year with 3 Pathologists and 3 Histologist. > > Anyone interested please contact Sue Wergin Laboratory Supervisor at > 262-656-5603. > > > > > _________________________________________________________________ > Windows Live SkyDrive lets you share files with faraway friends. > > http://www.windowslive.com/skydrive/overview.html?ocid=TXT_TAGLM_WL_Refresh_ skydrive_052008_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne (van Binsbergen) Hope Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Wed May 7 19:27:40 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed May 7 19:27:50 2008 Subject: [Histonet] Polymer IHC Detection Message-ID: <582736990805071727n10f00e42w643fadfd7f7b8d0f@mail.gmail.com> Hi, Does anyone know of a good polymer immunohistochemical detection kit that labels antibodise raised in animals other than mouse or rabbit? I am looking for a goat or rat detection kit. I know I could use a mouse anti goat then a mouse secondary, but I'd like to simplify the process. Of course vendor responses are more than welcome. Thanks, Amos Brooks From liz <@t> premierlab.com Wed May 7 19:34:18 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed May 7 19:34:29 2008 Subject: [Histonet] Polymer IHC Detection In-Reply-To: <582736990805071727n10f00e42w643fadfd7f7b8d0f@mail.gmail.com> References: <582736990805071727n10f00e42w643fadfd7f7b8d0f@mail.gmail.com> Message-ID: Biocare Medical has both of those. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Wednesday, May 07, 2008 6:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Polymer IHC Detection Hi, Does anyone know of a good polymer immunohistochemical detection kit that labels antibodise raised in animals other than mouse or rabbit? I am looking for a goat or rat detection kit. I know I could use a mouse anti goat then a mouse secondary, but I'd like to simplify the process. Of course vendor responses are more than welcome. Thanks, Amos Brooks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Wed May 7 19:48:35 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Wed May 7 19:48:43 2008 Subject: [Histonet] Polymer IHC Detection References: <582736990805071727n10f00e42w643fadfd7f7b8d0f@mail.gmail.com> Message-ID: <001801c8b0a5$3fb2e440$6501a8c0@DHXTS541> Amos, Biocare has more than one species, including goat and are single links, thankfully. Their rodent kit is for either rat or mouse. The kits can also be for either HRP or Alk Phos. We did tweak their protocol to fit our needs using a Alk Phos rabbit kit by eliminating a peroxidase blocking step they had in the AP protocol - since peroxidase was not in the staining loop anyway. Our staining went very well. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Amos Brooks" To: Sent: Wednesday, May 07, 2008 6:27 PM Subject: [Histonet] Polymer IHC Detection > Hi, > Does anyone know of a good polymer immunohistochemical detection kit > that labels antibodise raised in animals other than mouse or rabbit? I am > looking for a goat or rat detection kit. I know I could use a mouse anti > goat then a mouse secondary, but I'd like to simplify the process. Of > course > vendor responses are more than welcome. > > Thanks, > Amos Brooks > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Wed May 7 19:54:17 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed May 7 19:54:39 2008 Subject: [Histonet] c-kit (CD117) rat and mouse tissue Message-ID: <582736990805071754h7196855bwc7fabfedff82cbf3@mail.gmail.com> Hi, That's cool timing, I just finished a project using this today and it works great on mouse tissue. Unfortunately I cannot avouch for rat tissue. Using Dako's C-Kit, CD117, code A4502 with HIER and Envision Rabbit the results are very nice. Check out: http://www.dakousa.com/index/prod_search/prod_products.htm?productareaid=3&baseprodidver=A103500017 Good luck, Amos Brooks Message: 5 Date: Wed, 7 May 2008 13:56:46 -0400 From: michelle.schwab-macdonald@novartis.com Subject: [Histonet] c-kit (CD117) rat and mouse tissue To: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: < OF0A5C7F1D.F7CD11CD-ON85257442.006264AC-85257442.006294C4@ah.novartis.com> Content-Type: text/plain; charset="US-ASCII" I am looking for a c-kit Ab that will stain FFPE rat or mouse tissue. I have heard that Dako has one for rat. Is anyone using it and willing to share protocol information or does anyone have an Ab suggestion? Thanks in Advance!! :-) Michelle Schwab-MacDonald HT(ASCP) Novartis, Boston, MA From John.Auld <@t> whnt.nhs.uk Wed May 7 12:03:37 2008 From: John.Auld <@t> whnt.nhs.uk (John Auld) Date: Wed May 7 20:05:54 2008 Subject: [Histonet] John Auld/APH/WHT is out of the office. Message-ID: I will be out of the office starting 07/05/2008 and will not return until 27/05/2008. I will respond to your message when I return. Alternatively please contact carol.jones@whnt.nhs.uk or sharon.forrest@whnt.nhs.uk or contact them on ex 2560. From MichelM9 <@t> chw.edu.au Wed May 7 21:55:00 2008 From: MichelM9 <@t> chw.edu.au (Michelle McDonald) Date: Wed May 7 21:55:12 2008 Subject: [Histonet] Histozymmography Message-ID: <47BD6E7614A693499453835D47E36F70044219B8@hedwig.nch.kids> I would like to hear from anyone who has any experience with histozymmography on bone tissue sections. We are in the process of optimising a technique to analyse MMP activity in adult rat growth plates in bone samples. We have been able to cut frozen sections of decalcified samples but as yet have not perfected frozen sections of undecalcified samples even using a tungsten carbide blade. We are also currently coating slides with fluorescently labelled gelatin or casein dissolved in a 1% agarose in 50mM Tris Ph 7.2 solution at 37 degrees. It is difficult to get a consistent thick coating on the slide and under the microscope these coatings look grainy. If anyone has any suggestions on how we can optimise these techniques we would be greatly appreciative Thank you Kind Regards Michelle Dr Michelle McDonald B.MedSci, PhD Research Officer Orthopaedic Research Dept. The Children's Hospital Westmead. Westmead NSW 2145 Australia Ph. +612 9845 1451 Fax. +612 9845 3078 ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From annigyg <@t> gmail.com Wed May 7 22:51:52 2008 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Wed May 7 22:51:56 2008 Subject: [Histonet] Re: Too many Techs... In-Reply-To: <5F31F38C96781A4FBE3196EBC22D47807F2621@fhosxchmb006.ADVENTISTCORP.NET> References: <5F31F38C96781A4FBE3196EBC22D47807F2621@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: no, we do way less than 10 000 a year my point is that in my opinion, no matter how LOW the workload may be, there are several basic areas which need to be covered if one has to attend to all the listed 'peripheral' services, as well as cover the basic daily routine histo (and be the cyto backup!!) i KNOW my staff are busy because i know exactly what it takes to cover all the areas they are involved in but the boss is a 'number cruncher' and he see things through 'hematology' eyes and all is compared to them histo is very different breaks are taken with a clock running next to a coffee cup - lunch is eaten on the trot samples are collected in OR by techs and they do all the reception/accessioning/sorting out mistakes etc very time consuming but not really measurable, particularly if one is running around like a headless chicken dealing with irate surgeons looking for results!! so i am trying to get a handle on all your labs out there, what you actually do, *apart from basic histo*, and how many of you there are to do it do you have dedicated admin staff do you have filing clerks and accession/data entry staff does your AP section have a secretary to deal with clinicians demanding path reports do the lab staff (techs) have to deal with phone queries for results any/all feedback appreciated Annie ps when doing stats for headcounts, is IHC classified as a sub-section apart with its own staff and back up or is it counted with routine histo? 2008/5/7 Bonner, Janet : > 10,000cases? 3300 cases per tech per year, 100 cases per tech per > day? At least 300 blocks min. per day per tech. > CAP has guidelines for justifying positions. I would also have the > techs (like they need something else to do) keep track of their time for a > month. It sounds as if three techs would minimally handle your lab. Not to > mention two months worth of vacation/sick time taken per year. > Also - what is the boss seeing? Coffee breaks? Long lunch breaks? > We have 22 Techs, three shifts, six days per week, 50,000 Surgical > cases. It takes two techs worth just to cover time off per year. We do not > attend procedures and we buy our reagents. > Good luck. > ------------------------------ > *From:* histonet-bounces@lists.utsouthwestern.edu on behalf of Anne van > Binsbergen > *Sent:* Wed 5/7/2008 12:09 PM > *To:* barbara carter; histonet-request@lists.utsouthwestern.edu; Histonet > *Subject:* Re: [Histonet] Histology Coordinator Position in Kenosha > Wisconsin > > i have a few questions for you if i may. > you have 3 techs serving 3 paths and 10 000 a year > apart from the basic routine histo operations, do your 3 techs > > - do any grossing > - attend at renal biopsy collection > - do frozen sections > - take macro photos > - do slide and block filing > - source cases for sendout (consults/reviews) or do any archive > searches > - wash glassware, > - do specimen discards, > - make up formalin > > i am facing pressure from my (non-histo) boss who is comparing my lab and > staff to 'other modern facilities' and he now tells me i need to cut my > staff in half!! > i need ammo and numbers from others to build my defence > cheers > Annie > > 2008/5/7 barbara carter : > > > > > I am leaving my position as Coordinator at United Hospital System and am > > moving out of state and trying to help them recruit a new coordinator to > > take my place. > > > > There is a position open at United Hospital System at the Kenosha Campus > > in Kenosha Wisconsin for a Histology Coordinator. The hospital receives > > about 10,000 surgical specimen a year with 3 Pathologists and 3 > Histologist. > > > > Anyone interested please contact Sue Wergin Laboratory Supervisor at > > 262-656-5603. > > > > > > > > > > _________________________________________________________________ > > Windows Live SkyDrive lets you share files with faraway friends. > > > > > http://www.windowslive.com/skydrive/overview.html?ocid=TXT_TAGLM_WL_Refresh_skydrive_052008_______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Anne (van Binsbergen) Hope > Abu Dhabi > UAE > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ======================================================= > The information contained in this message may be privileged and/or confidential > and protected from disclosure. If the reader of this message is not the intended > recipient or an employee or agent responsible for delivering this message to the > intended recipient, you are hereby notified that any dissemination, distribution > or copying of this communication is strictly prohibited. If you have received this > communication in error, please notify the sender immediately by replying to this > message and deleting the material from any computer. > ======================================================= > > -- Anne (van Binsbergen) Hope Abu Dhabi UAE From Helen.Ilsley <@t> uct.ac.za Thu May 8 00:38:56 2008 From: Helen.Ilsley <@t> uct.ac.za (Helen Ilsley) Date: Thu May 8 00:39:12 2008 Subject: [Histonet] Antibody amplifier Message-ID: <85F52572-8E17-4BE7-8FE2-456512AF3B0E@uct.ac.za> Hi I was wondering if anyone has used the Antibody amplifier from Prohisto. I would love to know what people thought of it. Many thanks Helen Helen Ilsley Helen.Ilsley@uct.ac.za Cardiovascular Research Unit Cape Heart Centre Anzio Road, Observatory, UCT 021-406 6398/6590 021-448 5935 (fax) From ratkay <@t> telus.net Thu May 8 00:44:24 2008 From: ratkay <@t> telus.net (ratkay@telus.net) Date: Thu May 8 00:44:28 2008 Subject: [Histonet] Shandon Citadel Tissue Basket Message-ID: <1210225464.4822933879728@webmail.telus.net> Does anyone have an extra Tissue basket for the Citadel 1000? Ours is broken and would love to buy a used one. The new one is way too expensive unfortunately. Thank you, Leslie Ratkay Histoprobe Consulting From John.Spair <@t> multicare.org Thu May 8 01:54:46 2008 From: John.Spair <@t> multicare.org (John Spair) Date: Thu May 8 01:57:38 2008 Subject: [Histonet] RE: Histonet Digest, Vol 54, Issue 9 References: <643C96FA2KK9638-01@MMS_multicare.org> Message-ID: <61A9977919846C479389493BAE2517CA01530941@MHSEXMBX1.multicare.org> B plus is made by a company called BBC in Standwood, Washington. They have a web site you can visit. There are a few vendors that carry this product for them as well. I don't remember which ones off hand. However go to www.bbcus.com and check it out. John Spair, Manager Pathology Services LABORATORIES Northwest MultiCare Health System PO Box 5299, Tacoma WA 98415 Phone: 253-403-6090 Fax: 253-403-1357 Message: 2 Date: Wed, 7 May 2008 13:09:45 -0400 From: "Weber, Susan (VHACLE)" Subject: RE: [Histonet] B-plus question To: "Paul Bradbury" , "karen adams" , "HistoNet Server" Message-ID: <16C83872A53F4346AA9C3A18E3A3AAB903F76D96@VHAV10MSGA1.v10.med.va.gov> Content-Type: text/plain; charset="us-ascii" I may have missed this, but may I have the name of the Vendor you purchase your B-plus from. Thanks. Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 "MMS " made the following annotations. ------------------------------------------------------------------------------ NOTICE: This e-mail and the attachments hereto, if any, may contain privileged and/or confidential information. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, you are hereby notified that any examination, distribution or copying of this e-mail and the attachments hereto, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by email or telephone and permanently delete this e-mail and the attachments hereto, if any, and destroy any printout thereof. MultiCare Health System, Tacoma, WA 98415 (253) 403-1000. ============================================================================== From bakevictoria <@t> gmail.com Thu May 8 06:21:28 2008 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Thu May 8 06:21:33 2008 Subject: [Histonet] microwave pressure cookers Message-ID: <4f016b690805080421v60614df2y2d3034a29fe12df2@mail.gmail.com> Hi Everyone, I'm trying to locate a 'lab certified' microwaveable pressure cooker. The last one I saw I thought might have been from BioCare or Biogenex. Does anyone know who carries them or what you are using for microwave pressure cookers? Thanks - Vikki From petunia_c <@t> hotmail.com Thu May 8 06:47:50 2008 From: petunia_c <@t> hotmail.com (barbara carter) Date: Thu May 8 06:47:54 2008 Subject: [Histonet] Reposting Correct # for Kenosha WI Histo Position Message-ID: I am sorry I mistyped a number in the phone number for Sue Wergin Laboratory Supervisor 262-653-5603. I am posting a Histology Coordinator Position in Kenosha WI at United Hospital System. There is 3 Pathologist and 3 Histotechs plus the Coordinator. The Histology Lab gets about 10,000 specimen a year. Please call the above correct phone number for Sue Wergin. _________________________________________________________________ With Windows Live for mobile, your contacts travel with you. http://www.windowslive.com/mobile/overview.html?ocid=TXT_TAGLM_WL_Refresh_mobile_052008 From LSebree <@t> uwhealth.org Thu May 8 07:21:44 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Thu May 8 07:21:49 2008 Subject: [Histonet] microwave pressure cookers In-Reply-To: <4f016b690805080421v60614df2y2d3034a29fe12df2@mail.gmail.com> Message-ID: We use a couple of Decloaking Chambers (lab pressure cookers) from Biocare Medical and are quite satisfied with them. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Thursday, May 08, 2008 6:21 AM To: histonet Subject: [Histonet] microwave pressure cookers Hi Everyone, I'm trying to locate a 'lab certified' microwaveable pressure cooker. The last one I saw I thought might have been from BioCare or Biogenex. Does anyone know who carries them or what you are using for microwave pressure cookers? Thanks - Vikki _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmacdonald <@t> mtsac.edu Thu May 8 07:50:35 2008 From: jmacdonald <@t> mtsac.edu (jmacdonald@mtsac.edu) Date: Thu May 8 07:50:48 2008 Subject: [Histonet] microwave pressure cookers In-Reply-To: <4f016b690805080421v60614df2y2d3034a29fe12df2@mail.gmail.com> References: <4f016b690805080421v60614df2y2d3034a29fe12df2@mail.gmail.com> Message-ID: <1492979604-1210251037-cardhu_decombobulator_blackberry.rim.net-1755811456-@bxe017.bisx.prod.on.blackberry> Biocare or Cell Marque Sent via BlackBerry by AT&T -----Original Message----- From: "Victoria Baker" Date: Thu, 8 May 2008 06:21:28 To:histonet Subject: [Histonet] microwave pressure cookers Hi Everyone, I'm trying to locate a 'lab certified' microwaveable pressure cooker. The last one I saw I thought might have been from BioCare or Biogenex. Does anyone know who carries them or what you are using for microwave pressure cookers? Thanks - Vikki _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mickie25 <@t> netzero.net Thu May 8 08:22:23 2008 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Thu May 8 08:22:54 2008 Subject: [Histonet] Lot numbers In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA27056356BB@hpes1.HealthPartners.int> References: <0E394B648E5284478A6CCB78E5AFDA27056356BB@hpes1.HealthPartners.int> Message-ID: Dorothy, Regarding freezing compound coming off the chucks, this is usually a problem if the chucks are held in the cryostat before embedding. The mounting media does not get to the bottom of the groves and so does not stick. Start with a warm chuck when freezing, even when making a blank build-up. Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, May 07, 2008 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Lot numbers Does everyone keep track of each reagent lot number, including, water, formalin, alcohols, etc. as it is opened and used? What about recycled products? I am trying to come up with a logsheet and would appreciate any help in this area! Also, we have recently run into problems with our freezing compound coming off of the chucks during frozen sectioning. Any suggestions and/or what is the method of freezing tissue that everyone uses for their cryostat sections? Thanks fellow histotechs for helping me with these matters that I am coming up empty on!!!!!!!!!!!!!!!!!!!!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charles.Embrey <@t> carle.com Thu May 8 08:25:13 2008 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Thu May 8 08:25:17 2008 Subject: [Histonet] Too many Techs... In-Reply-To: <5F31F38C96781A4FBE3196EBC22D47807F2621@fhosxchmb006.ADVENTISTCORP.NET> References: <5F31F38C96781A4FBE3196EBC22D47807F2621@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: <44780C571F28624DBB446DE55C4D733A1FE5B6@EXCHANGEBE1.carle.com> Janet, I don't understand your math. 10,000 cases per year divided by 52 would be 192 cases a week divided between 3 techs would be 64 cases each tech per week and average 12.8 cases per tech per day. It doesn't sound too bad to me. 100 cases per tech per day (at 3 techs) would equal about 72,000 cases per year. Chuck -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonner, Janet Sent: Wednesday, May 07, 2008 12:06 PM To: Anne van Binsbergen; barbara carter; histonet-request@lists.utsouthwestern.edu; Histonet Subject: [Histonet] Too many Techs... 10,000cases? 3300 cases per tech per year, 100 cases per tech per day? At least 300 blocks min. per day per tech. CAP has guidelines for justifying positions. I would also have the techs (like they need something else to do) keep track of their time for a month. It sounds as if three techs would minimally handle your lab. Not to mention two months worth of vacation/sick time taken per year. Also - what is the boss seeing? Coffee breaks? Long lunch breaks? We have 22 Techs, three shifts, six days per week, 50,000 Surgical cases. It takes two techs worth just to cover time off per year. We do not attend procedures and we buy our reagents. Good luck. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Anne van Binsbergen Sent: Wed 5/7/2008 12:09 PM To: barbara carter; histonet-request@lists.utsouthwestern.edu; Histonet Subject: Re: [Histonet] Histology Coordinator Position in Kenosha Wisconsin i have a few questions for you if i may. you have 3 techs serving 3 paths and 10 000 a year apart from the basic routine histo operations, do your 3 techs - do any grossing - attend at renal biopsy collection - do frozen sections - take macro photos - do slide and block filing - source cases for sendout (consults/reviews) or do any archive searches - wash glassware, - do specimen discards, - make up formalin i am facing pressure from my (non-histo) boss who is comparing my lab and staff to 'other modern facilities' and he now tells me i need to cut my staff in half!! i need ammo and numbers from others to build my defence cheers Annie 2008/5/7 barbara carter : > > I am leaving my position as Coordinator at United Hospital System and am > moving out of state and trying to help them recruit a new coordinator to > take my place. > > There is a position open at United Hospital System at the Kenosha Campus > in Kenosha Wisconsin for a Histology Coordinator. The hospital receives > about 10,000 surgical specimen a year with 3 Pathologists and 3 Histologist. > > Anyone interested please contact Sue Wergin Laboratory Supervisor at > 262-656-5603. > > > > > _________________________________________________________________ > Windows Live SkyDrive lets you share files with faraway friends. > > http://www.windowslive.com/skydrive/overview.html?ocid=TXT_TAGLM_WL_Refr esh_skydrive_052008_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne (van Binsbergen) Hope Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Thu May 8 08:28:51 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu May 8 08:28:58 2008 Subject: [Histonet] Lot numbers In-Reply-To: References: <0E394B648E5284478A6CCB78E5AFDA27056356BB@hpes1.HealthPartners.int> Message-ID: We keep our chucks in the cryostat before sectioning and have never had this problem (cryotstat temp is -28C). It could be that fast freezing it is the problem. When we put oct on the chuck to attach the specimin, the oct gets into the grooves of the chuck and then freezes because we're not using the fast freeze mode. Also we use Tissue Tek oct, if name brand makes a difference. Emily -- If you're a cowboy and you're dragging a guy behind your horse, I bet it would really make you mad if you looked back and the guy was reading a magazine. From bakevictoria <@t> gmail.com Thu May 8 08:48:23 2008 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Thu May 8 08:48:28 2008 Subject: [Histonet] Re: microwave pressure cookers In-Reply-To: <4f016b690805080421v60614df2y2d3034a29fe12df2@mail.gmail.com> References: <4f016b690805080421v60614df2y2d3034a29fe12df2@mail.gmail.com> Message-ID: <4f016b690805080648vd7fbcbbva085796294d22ae8@mail.gmail.com> Thanks - I appreciate your in put. I tried looking at BioCare's website and the pressure cooker I saw wasn't for the microwave. Can someone give me a product code number? I apologize, but I don't think I'm looking in the right place on their website. Thank you all again. Vikki On 5/8/08, Victoria Baker wrote: > Hi Everyone, > > I'm trying to locate a 'lab certified' microwaveable pressure cooker. > The last one I saw I thought might have been from BioCare or Biogenex. > Does anyone know who carries them or what you are using for microwave > pressure cookers? > > Thanks - > > Vikki > From LSebree <@t> uwhealth.org Thu May 8 08:54:05 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Thu May 8 08:54:09 2008 Subject: [Histonet] Re: microwave pressure cookers In-Reply-To: <4f016b690805080648vd7fbcbbva085796294d22ae8@mail.gmail.com> Message-ID: I'm sorry Vikki, I didn't understand that you wanted a pressure cooker for the MW. If you have the option, the stand alone lab pressure cookers (such as the Decloaking Chamber) work really well. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Thursday, May 08, 2008 8:48 AM To: histonet Subject: [Histonet] Re: microwave pressure cookers Thanks - I appreciate your in put. I tried looking at BioCare's website and the pressure cooker I saw wasn't for the microwave. Can someone give me a product code number? I apologize, but I don't think I'm looking in the right place on their website. Thank you all again. Vikki On 5/8/08, Victoria Baker wrote: > Hi Everyone, > > I'm trying to locate a 'lab certified' microwaveable pressure cooker. > The last one I saw I thought might have been from BioCare or Biogenex. > Does anyone know who carries them or what you are using for microwave > pressure cookers? > > Thanks - > > Vikki > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu May 8 09:51:06 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 8 09:51:11 2008 Subject: [Histonet] Re; Decal Process From the Past In-Reply-To: <251486.25591.qm@web31304.mail.mud.yahoo.com> Message-ID: <518733.53912.qm@web65714.mail.ac4.yahoo.com> About this "washing after DECAL" issue I would like to call the attention to the fact that, even if the acid decal solutions are usually strong acids, the amount of carryover to the first formalin station is so minuscule that there is no possibility of a pH change in it, specially considering the fact that it is a neutral BUFFERED formalin solution. That is why after our decals we just washed the specimens in running water for no more than 5 minutes and we had no problems. Ren? J. Akemi Allison-Tacha wrote: --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From asmith <@t> mail.barry.edu Thu May 8 11:37:43 2008 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Thu May 8 11:37:55 2008 Subject: [Histonet] RE: glassware cleaners In-Reply-To: <57BE698966D5C54EAE8612E8941D768302E38DDF@EXCHANGE3.huntingtonhospital.com> References: <57BE698966D5C54EAE8612E8941D768302E38DDF@EXCHANGE3.huntingtonhospital.com> Message-ID: For silver deposits, I use concentrated nitric acid. For blood, I use Alconox. For everything else, I use Eradastain (made by Cambridge Diagnostics, sold by Sigma-Aldrich). -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Wednesday, May 07, 2008 5:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] glassware cleaners I have used a detergent called Contrad 70 for years to clean my glassware, but it is no longer available from my vendor. What are others using to clean iron solutions, muci stain, silver, etc off of their glassware? Laurie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Robert.Lott <@t> TriadHospitals.com Thu May 8 11:58:15 2008 From: Robert.Lott <@t> TriadHospitals.com (Lott, Robert) Date: Thu May 8 11:58:22 2008 Subject: [Histonet] Bone Flap from Skull Message-ID: <4A3619571D9F6C4CB79C980E91DBE4E61FAC94@TNTRIEXEVS03.triadhospitals.net> A neurosurgeon just brought a bone flap from a patient and wants to replace it 3-4 days from now... Do any of you have suggestions or recommendations for storing the bone flap in the lab until he needs it again??? Need help fast! Thanks! Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology Trinity Medical Center / LabFirst 800 Montclair Road Birmingham, AL 35213 205-592-5388 205-592-5646 - fax robert.lott@triadhospitals.com From asmith <@t> mail.barry.edu Thu May 8 11:59:36 2008 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Thu May 8 11:59:47 2008 Subject: [Histonet] histonet@lists.utsouthwestern.edu In-Reply-To: <050720082100.4274.48221883000BDFE3000010B22216557996C7CD0C0E070B0196@comcast.net> References: <050720082100.4274.48221883000BDFE3000010B22216557996C7CD0C0E070B0196@comcast.net> Message-ID: Plain silver nitrate is not a problem. Diammine silver complexes can explode if they dry out, because they consist of an oxidizing agent (silver ion) complexed with a reducing agent (ammonia). The decomposition of diammine silver in solution is complex and poorly understood, but it seems to produce hydrogen gas, which is explosive. One might store diammine silver hydroxide in an explosion-proof refrigerator overnight, but it would be unwise to store it any longer than that. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of zodiac29@comcast.net Sent: Wednesday, May 07, 2008 5:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] histonet@lists.utsouthwestern.edu Hello, I have read somewhere that silver solutions should be stored in an expolsive proof refrigerator. Is this true? Right know we just store them in a regular house hold type fridge. Can anyone comment on this? Thanks, Jenny _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Thu May 8 12:02:34 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Thu May 8 12:02:39 2008 Subject: [Histonet] Bone Flap from Skull In-Reply-To: <4A3619571D9F6C4CB79C980E91DBE4E61FAC94@TNTRIEXEVS03.triadhospitals.net> Message-ID: Just off the top of my head (no pun intended) I would think an organ preservation solution might be reasonable assuming that your institution is involved in transplantation. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lott, Robert Sent: Thursday, May 08, 2008 11:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bone Flap from Skull A neurosurgeon just brought a bone flap from a patient and wants to replace it 3-4 days from now... Do any of you have suggestions or recommendations for storing the bone flap in the lab until he needs it again??? Need help fast! Thanks! Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology Trinity Medical Center / LabFirst 800 Montclair Road Birmingham, AL 35213 205-592-5388 205-592-5646 - fax robert.lott@triadhospitals.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu May 8 12:15:14 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 8 12:15:19 2008 Subject: [Histonet] Bone Flap from Skull In-Reply-To: <4A3619571D9F6C4CB79C980E91DBE4E61FAC94@TNTRIEXEVS03.triadhospitals.net> Message-ID: <160753.70968.qm@web65705.mail.ac4.yahoo.com> Freeze it at -80?C Ren? J. "Lott, Robert" wrote: A neurosurgeon just brought a bone flap from a patient and wants to replace it 3-4 days from now... Do any of you have suggestions or recommendations for storing the bone flap in the lab until he needs it again??? Need help fast! Thanks! Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology Trinity Medical Center / LabFirst 800 Montclair Road Birmingham, AL 35213 205-592-5388 205-592-5646 - fax robert.lott@triadhospitals.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From mcauliff <@t> umdnj.edu Thu May 8 12:16:51 2008 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu May 8 12:16:44 2008 Subject: [Histonet] Bone Flap from Skull In-Reply-To: References: Message-ID: <48233583.3050809@umdnj.edu> You do not need help with this, you need to get far away from this. I can't believe your institution does not have a protocol for this! Are you really going to take recommendations from Histoneters who are not MDs? I can just see you testifying at the malpractice trail (after the infected/necrotic tissue causes serious illness or death) that someone on histonet told you it was OK to ........... Get my drift? This is obvious malpractice on the surgeon's part. Run like hell. Geoff > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lott, > Robert > Sent: Thursday, May 08, 2008 11:58 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Bone Flap from Skull > > > A neurosurgeon just brought a bone flap from a patient and wants to > replace it 3-4 days from now... > > Do any of you have suggestions or recommendations for storing the bone > flap in the lab until he needs it again??? > > > > Need help fast! > > > > Thanks! > > > > > > Robert L. Lott, HTL(ASCP) > > Manager, Anatomic Pathology > > Trinity Medical Center / LabFirst > > 800 Montclair Road > > Birmingham, AL 35213 > > 205-592-5388 > > 205-592-5646 - fax > > robert.lott@triadhospitals.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From rachelr <@t> mail.nih.gov Thu May 8 12:18:57 2008 From: rachelr <@t> mail.nih.gov (Rivka Rachel) Date: Thu May 8 12:19:53 2008 Subject: [Histonet] Bone Flap from Skull In-Reply-To: <48233583.3050809@umdnj.edu> Message-ID: As a former neurosurgeon myself, I second that vote. Rivka On 5/8/08 1:16 PM, "Geoff McAuliffe" wrote: > You do not need help with this, you need to get far away from this. > I can't believe your institution does not have a protocol for this! > Are you really going to take recommendations from Histoneters who are > not MDs? > I can just see you testifying at the malpractice trail (after the > infected/necrotic tissue causes serious > illness or death) that someone on histonet told you it was OK to ........... > Get my drift? > This is obvious malpractice on the surgeon's part. Run like hell. > > Geoff >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lott, >> Robert >> Sent: Thursday, May 08, 2008 11:58 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Bone Flap from Skull >> >> >> A neurosurgeon just brought a bone flap from a patient and wants to >> replace it 3-4 days from now... >> >> Do any of you have suggestions or recommendations for storing the bone >> flap in the lab until he needs it again??? >> >> >> >> Need help fast! >> >> >> >> Thanks! >> >> >> >> >> >> Robert L. Lott, HTL(ASCP) >> >> Manager, Anatomic Pathology >> >> Trinity Medical Center / LabFirst >> >> 800 Montclair Road >> >> Birmingham, AL 35213 >> >> 205-592-5388 >> >> 205-592-5646 - fax >> >> robert.lott@triadhospitals.com >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > -- Rivka A. Rachel, MD, PhD Staff Scientist, National Eye Institute Neurobiology, Neurodegeneration & Repair Laboratory Bldg 10 Rm 10D43 Tel: 301 443-4906 From Erin.Martin <@t> ucsf.edu Thu May 8 12:26:13 2008 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Thu May 8 12:29:57 2008 Subject: [Histonet] California licensure Message-ID: Hi everyone, FYI - I finally got a call back from Lab Field Services with the CA dept of health. There is no California license for histotechs and she did not know of any plans for one. She thought that perhaps there was some confusion between histocompatibility tech (license) and histotech (no license). Erin From BoozerKA <@t> ah.org Thu May 8 12:57:00 2008 From: BoozerKA <@t> ah.org (Kathleen Boozer) Date: Thu May 8 12:57:40 2008 Subject: [Histonet] (no subject) Message-ID: <4822DC7C.4AA8.00C0.0@ah.org> From Marirose.Satterfield <@t> MercyMemorial.org Thu May 8 13:06:38 2008 From: Marirose.Satterfield <@t> MercyMemorial.org (Satterfield, Marirose) Date: Thu May 8 13:06:48 2008 Subject: [Histonet] CAP Her2Neu regs Message-ID: I am trying to comply with the CAP Her2Neu guidelines. I am thinking about letting our weekend specimens set in 70 % alcohol after 24 hrs of Formalin fixation. I was just wondering if I could have some feedback from anyone who uses this method. Does it effect your small biopsies when they are run this way? M Satterfield Histology Supervisor Mercy Memorial Hospital From leiker <@t> buffalo.edu Thu May 8 14:14:59 2008 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Thu May 8 14:15:05 2008 Subject: [Histonet] Why use Calcium Chloride in Trypsin Antigen Retrieval? Message-ID: <4C54C915415D9C933626AEA2@bchwxp2702.ad.med.buffalo.edu> Does anyone know why CaCl2 is used in trypsin buffers for retrieval of antigen in paraformaldehyde-fixed, paraffin-embedded tissue sections? Is it really necessary? Can I just use a Trypsin-EDTA solution we already have in the lab? Merced M Leiker Research Technician II 354 BRB (Lee Lab) / 140 Farber Hall (mail) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 From Carmen.Wynn <@t> us.astellas.com Thu May 8 14:21:56 2008 From: Carmen.Wynn <@t> us.astellas.com (Wynn, Carmen) Date: Thu May 8 14:22:33 2008 Subject: [Histonet] RE: Polymer IHC Detection In-Reply-To: <20080508003841.5FE3AC300D6@mail2-wa4.bigfish.com> References: <20080508003841.5FE3AC300D6@mail2-wa4.bigfish.com> Message-ID: Hello Amos, I have used the Biocare Medical Goat HRP polymer Kit, which detects Goat primary antibodies for use on Mouse, Rat, and Human tissue. Catalog# is GHP516G. Be sure to use some sort of detergent in your wash buffer to prevent background from ionic interactions on the slides. I use 0.05% Tween 20 in my wash buffer solution for any Biocare HRP polymer product and the last step prior to the substrate chromagen should always be a rinse in deionized water. Coincidently, my wash buffer is also my antibody diluent. Hope that this helps. Surely, you could by there special TBS buffer but it's more economical for me to make my own and add the Tween 20. Carmen Wynn, M.S., Senior Scientist Astellas Research Institute of America, LLC. (ARIA) Illinois Science and Technology Park 8045 Lamon Ave Skokie, IL 60077 Direct: 847-933-7419 Main: 847-933-7400 Fax: 847-933-7401 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, May 07, 2008 7:39 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 54, Issue 9 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Too many Techs... (Bonner, Janet) 2. RE: B-plus question (Weber, Susan (VHACLE)) 3. Re: cryoprotection (John Kiernan) 4. Lot numbers (Webb, Dorothy L) 5. c-kit (CD117) rat and mouse tissue (michelle.schwab-macdonald@novartis.com) 6. Shirley Phua is out-of-office ... (Shirley PHUA) 7. RE: GLP question (Trajkovic, Dusko) 8. RE: Lot numbers. . (Henry, Charlene) 9. RE: B-plus question. . (Henry, Charlene) 10. Immunohistotechnologist position open at Kansas State Univ. (Shelly Christenson) 11. RE: Lot numbers. . (Rene J Buesa) 12. RE: Lot numbers (Bonner, Janet) 13. RE: c-kit (CD117) rat and mouse tissue (Wright, Clarissa B CIV) 14. histonet@lists.utsouthwestern.edu (zodiac29@comcast.net) 15. glassware cleaners (Laurie Colbert) 16. RE: More on [Histonet] Looking for detailed protocol to stain for PASin liver sections (paraffin) (Tony Henwood) 17. RE: More on [Histonet] Looking for detailed protocol to stain for PASin liver sections (paraffin) (Tony Henwood) 18. Re: Re; Decal Process From the Past (Akemi Allison-Tacha) 19. RE: Too many Techs... (jstaruk) 20. Polymer IHC Detection (Amos Brooks) ---------------------------------------------------------------------- Message: 1 Date: Wed, 7 May 2008 13:06:17 -0400 From: "Bonner, Janet" Subject: [Histonet] Too many Techs... To: "Anne van Binsbergen" , "barbara carter" , histonet-request@lists.utsouthwestern.edu, "Histonet" Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2621@fhosxchmb006.ADVENTISTCORP.NET> Content-Type: text/plain; charset=iso-8859-1 10,000cases? 3300 cases per tech per year, 100 cases per tech per day? At least 300 blocks min. per day per tech. CAP has guidelines for justifying positions. I would also have the techs (like they need something else to do) keep track of their time for a month. It sounds as if three techs would minimally handle your lab. Not to mention two months worth of vacation/sick time taken per year. Also - what is the boss seeing? Coffee breaks? Long lunch breaks? We have 22 Techs, three shifts, six days per week, 50,000 Surgical cases. It takes two techs worth just to cover time off per year. We do not attend procedures and we buy our reagents. Good luck. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Anne van Binsbergen Sent: Wed 5/7/2008 12:09 PM To: barbara carter; histonet-request@lists.utsouthwestern.edu; Histonet Subject: Re: [Histonet] Histology Coordinator Position in Kenosha Wisconsin i have a few questions for you if i may. you have 3 techs serving 3 paths and 10 000 a year apart from the basic routine histo operations, do your 3 techs - do any grossing - attend at renal biopsy collection - do frozen sections - take macro photos - do slide and block filing - source cases for sendout (consults/reviews) or do any archive searches - wash glassware, - do specimen discards, - make up formalin i am facing pressure from my (non-histo) boss who is comparing my lab and staff to 'other modern facilities' and he now tells me i need to cut my staff in half!! i need ammo and numbers from others to build my defence cheers Annie 2008/5/7 barbara carter : > > I am leaving my position as Coordinator at United Hospital System and am > moving out of state and trying to help them recruit a new coordinator to > take my place. > > There is a position open at United Hospital System at the Kenosha Campus > in Kenosha Wisconsin for a Histology Coordinator. The hospital receives > about 10,000 surgical specimen a year with 3 Pathologists and 3 Histologist. > > Anyone interested please contact Sue Wergin Laboratory Supervisor at > 262-656-5603. > > > > > _________________________________________________________________ > Windows Live SkyDrive lets you share files with faraway friends. > > http://www.windowslive.com/skydrive/overview.html?ocid=TXT_TAGLM_WL_Refr esh_skydrive_052008_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne (van Binsbergen) Hope Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= ------------------------------ Message: 2 Date: Wed, 7 May 2008 13:09:45 -0400 From: "Weber, Susan (VHACLE)" Subject: RE: [Histonet] B-plus question To: "Paul Bradbury" , "karen adams" , "HistoNet Server" Message-ID: <16C83872A53F4346AA9C3A18E3A3AAB903F76D96@VHAV10MSGA1.v10.med.va.gov> Content-Type: text/plain; charset="us-ascii" I may have missed this, but may I have the name of the Vendor you purchase your B-plus from. Thanks. Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paul Bradbury Sent: Tuesday, May 06, 2008 9:53 AM To: karen adams; HistoNet Server Subject: Re: [Histonet] B-plus question Hi Karen, I have been using the same sequence of reagents for several years with great success. We routinely fix bone marrow cores for 3 hours in B-plus, rinse in water, decalcify, rinse again, and put the cassette in with all the other tissues for processing. B-plus contains formaldehyde anyway, so your are not introducing a different reagent when you transfer them to formalin during processing. B-plus gives much better cytological detail than formalin. Nuclear detail is crisper, granules are better preserved, hemosiderin is not effected. CD-3, CD-20, kappa and lambda, etc. all work beautifully after B-plus. It also has the advantage of not producing an fixation artefact pigment like B-5 does. I think you will be happy with your decision to change. Paul Bradbury Kamloops, Canada karen adams wrote: > Hello all....we are changing bone marrow fixative from formalin to B-plus. > If we fix for the required time in B-plu and then process on the VIP w/ the > other specimens using formalin are there any effects on the tissue going > from B-plus to formalin?? > Thank you in advance > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Wed, 07 May 2008 13:24:29 -0400 From: John Kiernan Subject: Re: [Histonet] cryoprotection To: Teisha Robertson Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="us-ascii" Transfer the brain, after adequate fixation in buffered forma brain sin cutting sections. should still freeze as q on a metal cryostat chuck, compound for adhesion, then stand the chu CO2 and either acetone or alcohol. Mount the c in the cryostat and wait for its temperature  equilibrate. This isn't the fastest way to freeze thing but it's good enough for cryoprotected formaldehyde-fixed rodent brain UWO

----- Or <tshrobertso May 5, 2008 12:41< cryoprotection
To: histonet@li sts.utsouthwestern.edu

> i am trying to cryopr
> brain in sucrose. can you please
to prevent holes in my sample?
> ---------------------------------
> Be a
> ______ _______________________ 5F _________________
> Hist Histonet@lists.utsouthwestern.ed http://lists.utsouthwestern.edu/mailman/listinfo ------------------------------ Message: 4 Date: Wed, 07 May 2008 12:56:24 -0500 From: "Webb, Dorothy L" Subject: [Histonet] Lot numbers To: histonet@lists.utsouthwestern.edu Message-ID: <0E394B648E5284478A6CCB78E5AFDA27056356BB@hpes1.HealthPartners.int> Content-Type: text/plain; charset="us-ascii" Does everyone keep track of each reagent lot number, including, water, formalin, alcohols, etc. as it is opened and used? What about recycled products? I am trying to come up with a logsheet and would appreciate any help in this area! Also, we have recently run into problems with our freezing compound coming off of the chucks during frozen sectioning. Any suggestions and/or what is the method of freezing tissue that everyone uses for their cryostat sections? Thanks fellow histotechs for helping me with these matters that I am coming up empty on!!!!!!!!!!!!!!!!!!!!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. ------------------------------ Message: 5 Date: Wed, 7 May 2008 13:56:46 -0400 From: michelle.schwab-macdonald@novartis.com Subject: [Histonet] c-kit (CD117) rat and mouse tissue To: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" I am looking for a c-kit Ab that will stain FFPE rat or mouse tissue. I have heard that Dako has one for rat. Is anyone using it and willing to share protocol information or does anyone have an Ab suggestion? Thanks in Advance!! :-) Michelle Schwab-MacDonald HT(ASCP) Novartis, Boston, MA ------------------------------ Message: 6 Date: Thu, 8 May 2008 02:02:51 +0800 From: Shirley PHUA Subject: [Histonet] Shirley Phua is out-of-office ... To: histonet Message-ID: Content-Type: text/plain; charset=US-ASCII I will be out of the office from 08-05-2008 to 09-05-2008. I'll be away on 08 May 2008. Pathologists: I will process your requests when I return. If urgent, please forward your email to Henry_Kyaw@hsa.gov.sg ------------------------------ Message: 7 Date: Wed, 7 May 2008 11:16:21 -0700 From: "Trajkovic, Dusko" Subject: RE: [Histonet] GLP question To: "Jackie M O'Connor" Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu, Linke_Noelle Message-ID: <3AD0BD3142459B4E9B12CBEAFF2B89B2070C2917@lajamrexm01.amer.pfizer.com> Content-Type: text/plain; charset="us-ascii" If our lab had the same operation as Jackie O., then that is the way I would also perform the GLP tracking. However, our set up is similar to Kim Merriam, where each Histotech is responsible for their own study or work assigned. One person will do the trimming, embedding, sectioning, staining and self QC. I don't consider my slides as final product until I sign off on the QC portion, have someone else verify and initial that my numbers are correct (blocks and slides match and are all accounted for) then hand the slides to the pathologist. During my QC process, if I find that there are imperfections in the sections (this is hypothetical, since things like that never happen to me) such as folds, knife marks, floaters, etc. I go back to recut the slide, restain and QC again. If I am satisfied with my new freshly coverslipped slide, I sign off the animal and submit my slides. The slide that I discarded is nothing more than any other section that I discarded during my microtome sectioning before deciding that I am deep enough in the block and it's time to collect my ribbon and place on the water bath. This is my story, and my opinion on the GLP process. I'm sure that someone else might have some issues with my process, but every situation is different. Having worked in a GLP lab for quite a few years, never had any issues with FDA inspectors regarding any work that I have submitted. Dusko Trajkovic Pfizer Inc, La Jolla ________________________________ From: Jackie M O'Connor [mailto:Jackie.O'Connor@abbott.com] Sent: Wednesday, May 07, 2008 5:39 AM To: Trajkovic, Dusko Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu; Kim Merriam; Linke_Noelle Subject: RE: [Histonet] GLP question My lab has the capability of producing 600+ slides per day. When we cut initial slides, the microtomy is documented by the microtomist at the time. Staining is subsequently documented - since it may be performed by another technician. Once documentation of a procedural step is made, that slide is already raw datum. If a recut is needed at QC, the reason for the recut is also documented, and the original slide is retained. The recut is subsequently documented, as well as whether or not the desired goal (missing mammary) was achieved. Gotta love the world of GLP. We may be documenting this to death - but it works quite well. ------------------------------ Message: 8 Date: Wed, 7 May 2008 14:53:42 -0500 From: "Henry, Charlene" Subject: RE: [Histonet] Lot numbers. . To: "'Webb, Dorothy L'" , "histonet@lists.utsouthwestern.edu" Message-ID: <03E1F5968F60C5448635D49D38B283ED797B597D@SJMEMXMBS11.stjude.sjcrh.local > Content-Type: text/plain; charset="us-ascii" We had put the tracking of lot numbers and expiration dates of reagents (alcohol, xylene, methanol, formalin etc) into place just before our last CAP inspection and I'm glad we did because we would have been sited with a deficiency. I don't think that it is on the AP Checklist but it is on the General Checklist. The inspector asking me about the reagent lot numbers and expiration dates was a chemistry person and I tried to explain to her that the volume of reagents that a Histology Lab goes through in comparison with a Chemistry Lab is quite different. I told her that a Histology Lab would go through more reagents than the rest of all the clinical labs put together. I would think it would be impossible to track the lot number of recycled alcohols. We don't track the lot numbers of water because we have a Millipore system. We do track the lot number of formalin as it comes in and is put into use; however we do not track which lot number of formalin is used on individual cases. My experience with the compound coming off chucks during frozen sections has been when the chuck is too cold so we do not store our chucks in the cryostat but keep them at room temperature. It really does not add that much time when freezing tissue. Charlene Henry HT (ASCP), QIHC Anatomic Pathology Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, May 07, 2008 12:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Lot numbers. . Does everyone keep track of each reagent lot number, including, water, formalin, alcohols, etc. as it is opened and used? What about recycled products? I am trying to come up with a logsheet and would appreciate any help in this area! Also, we have recently run into problems with our freezing compound coming off of the chucks during frozen sectioning. Any suggestions and/or what is the method of freezing tissue that everyone uses for their cryostat sections? Thanks fellow histotechs for helping me with these matters that I am coming up empty on!!!!!!!!!!!!!!!!!!!!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Wed, 7 May 2008 14:58:49 -0500 From: "Henry, Charlene" Subject: RE: [Histonet] B-plus question. . To: "'Weber, Susan (VHACLE)'" , Paul Bradbury , karen adams , HistoNet Server Message-ID: <03E1F5968F60C5448635D49D38B283ED797B597F@SJMEMXMBS11.stjude.sjcrh.local > Content-Type: text/plain; charset="us-ascii" We get our B-plus from BBC Biomedical. Charlene -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weber, Susan (VHACLE) Sent: Wednesday, May 07, 2008 12:10 PM To: Paul Bradbury; karen adams; HistoNet Server Subject: RE: [Histonet] B-plus question. . I may have missed this, but may I have the name of the Vendor you purchase your B-plus from. Thanks. Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paul Bradbury Sent: Tuesday, May 06, 2008 9:53 AM To: karen adams; HistoNet Server Subject: Re: [Histonet] B-plus question Hi Karen, I have been using the same sequence of reagents for several years with great success. We routinely fix bone marrow cores for 3 hours in B-plus, rinse in water, decalcify, rinse again, and put the cassette in with all the other tissues for processing. B-plus contains formaldehyde anyway, so your are not introducing a different reagent when you transfer them to formalin during processing. B-plus gives much better cytological detail than formalin. Nuclear detail is crisper, granules are better preserved, hemosiderin is not effected. CD-3, CD-20, kappa and lambda, etc. all work beautifully after B-plus. It also has the advantage of not producing an fixation artefact pigment like B-5 does. I think you will be happy with your decision to change. Paul Bradbury Kamloops, Canada karen adams wrote: > Hello all....we are changing bone marrow fixative from formalin to B-plus. > If we fix for the required time in B-plu and then process on the VIP w/ the > other specimens using formalin are there any effects on the tissue going > from B-plus to formalin?? > Thank you in advance > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Wed, 07 May 2008 15:02:31 -0500 From: "Shelly Christenson" Subject: [Histonet] Immunohistotechnologist position open at Kansas State Univ. To: Message-ID: <4821C486.EF61.003F.0@vet.k-state.edu> Content-Type: text/plain; charset=US-ASCII There is a position in the Immunohistochemistry section of the Histology Lab of the Kansas State Veterinary Diagnostic Laboratory. The successful candidate will have a background in Immunohictochemeistry, Immunofluorescence, and other aspects of histology . Supervisory experience will be beneficial. A Bachelor of Science or equivalent degree and a minimum of one year of laboratory experience is required. This is just a short version of the job description for more information can contact: Dr. Brad DeBey, DVM, PhD 785/532-4481 debey@vet.k-state.edu Also it posited online https://www.da.ks.gov/ps/esummary/es-online/frmes1.asp Requisition number is 160443 Feel free to contact me also. Shelly Christenson HT(ASCP) 785/532-4464 christen@vet.k-state.edu ------------------------------ Message: 11 Date: Wed, 7 May 2008 13:03:32 -0700 (PDT) From: Rene J Buesa Subject: RE: [Histonet] Lot numbers. . To: "Henry, Charlene" , "'Webb, Dorothy L'" , "histonet@lists.utsouthwestern.edu" Message-ID: <314908.74685.qm@web65707.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 The only lot numbers I kept track of were those of "critical reagents", namely: any one dealing with any IHC procedure and the few staining solutions bought commercially. Ren J. "Henry, Charlene" wrote: --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. ------------------------------ Message: 12 Date: Wed, 7 May 2008 16:08:49 -0400 From: "Bonner, Janet" Subject: RE: [Histonet] Lot numbers To: "Webb, Dorothy L" , histonet@lists.utsouthwestern.edu Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F262B@fhosxchmb006.ADVENTISTCORP.NET> Content-Type: text/plain; charset=iso-8859-1 We have this happen when the chuck is the temperature of the cryostat before we put the OCT on it. The OCT freezes before it has a chance to seep into the grooves. Try putting the OCT on the warm chuck just after you put the chuck on the bar in the cryostat. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Webb, Dorothy L Sent: Wed 5/7/2008 1:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Lot numbers Does everyone keep track of each reagent lot number, including, water, formalin, alcohols, etc. as it is opened and used? What about recycled products? I am trying to come up with a logsheet and would appreciate any help in this area! Also, we have recently run into problems with our freezing compound coming off of the chucks during frozen sectioning. Any suggestions and/or what is the method of freezing tissue that everyone uses for their cryostat sections? Thanks fellow histotechs for helping me with these matters that I am coming up empty on!!!!!!!!!!!!!!!!!!!!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= ------------------------------ Message: 13 Date: Wed, 7 May 2008 13:13:04 -0700 From: "Wright, Clarissa B CIV" Subject: RE: [Histonet] c-kit (CD117) rat and mouse tissue To: , , Message-ID: <8072C1D1C2865C49B7F0A95FC47B7646AABF35@NMCSD-EX-VS-01.nmed.ds.med.navy. mil> Content-Type: text/plain; charset="us-ascii" Michelle, Try Lifespan Biosciences, they have several. I have not tried them, so I can't help you with the protocol. Kris -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of michelle.schwab-macdonald@novartis.com Sent: Wednesday, May 07, 2008 10:57 AM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] c-kit (CD117) rat and mouse tissue I am looking for a c-kit Ab that will stain FFPE rat or mouse tissue. I have heard that Dako has one for rat. Is anyone using it and willing to share protocol information or does anyone have an Ab suggestion? Thanks in Advance!! :-) Michelle Schwab-MacDonald HT(ASCP) Novartis, Boston, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Wed, 07 May 2008 21:00:51 +0000 From: zodiac29@comcast.net Subject: [Histonet] histonet@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Message-ID: <050720082100.4274.48221883000BDFE3000010B22216557996C7CD0C0E070B0196@co mcast.net> Content-Type: text/plain Hello, I have read somewhere that silver solutions should be stored in an expolsive proof refrigerator. Is this true? Right know we just store them in a regular house hold type fridge. Can anyone comment on this? Thanks, Jenny ------------------------------ Message: 15 Date: Wed, 7 May 2008 14:11:39 -0700 From: "Laurie Colbert" Subject: [Histonet] glassware cleaners To: Message-ID: <57BE698966D5C54EAE8612E8941D768302E38DDF@EXCHANGE3.huntingtonhospital.c om> Content-Type: text/plain; charset="us-ascii" I have used a detergent called Contrad 70 for years to clean my glassware, but it is no longer available from my vendor. What are others using to clean iron solutions, muci stain, silver, etc off of their glassware? Laurie ------------------------------ Message: 16 Date: Thu, 8 May 2008 09:17:57 +1000 From: "Tony Henwood" Subject: RE: More on [Histonet] Looking for detailed protocol to stain for PASin liver sections (paraffin) To: "Gayle Callis" , "Histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" I have found the following Modified PAS procedure especially useful for cytology smears that have been fixed in ethanol: Alcoholic PAS Stain Mucins and glycogen are water soluble. Over-rinsing slides, especially cytological smears, could result in excessive loss of these PAS substances. The following variant of the PAS stain performs the reactions in alcohol, thus decreasing the loss of these substances. Solutions: 1. Alcoholic Schiff reagent Basic Fuchsin 0.5g Ethanol 80ml Distilled Water 20ml Hydrochloric acid 1ml 2. Alcoholic Periodic acid Periodic acid 0.5g 95% ethanol 50ml Procedure: 1. Fix smears in 95% ethanol or use methanol fixed air-dried smears. 2. Place in alcoholic periodic acid 20min. 3. Wash in alcohol 4. Place in alcoholic Schiff's 20min. 5. Rinse slides in alcohol to remove excess dye. 6. Rinse slides in water. 7. Counterstain slides in Haematoxylin 2min. 8. Rinse slides in water. 9. Differentiate and Blue. 10. Dehydrate, clear and mount. References: Horobin and Kevill-Davis (1971) Stain Techn 46:53-58. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Thursday, 8 May 2008 2:06 AM To: Histonet Subject: Fw: More on [Histonet] Looking for detailed protocol to stain for PASin liver sections (paraffin) Dr. Nasonkin was staining for glycogen (per my question) and via private emailing, it was suggested he not use aqueous formalin or PFA fixation, but an alcoholic fixative to retain the glycogen in the liver, do diastase digestion to prove it is glyogen, and also process tissues - starting in higher (100%) alcohols to help retain glycogen. Suggested fixatives were Carnoy, Gendre and alcoholic formalin. If you have any other suggestions for him, please add to the list of to do's . Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT > ----- Original Message ----- > From: "Igor Nasonkin" > To: "Gayle Callis" > Cc: > Sent: Tuesday, May 06, 2008 6:35 PM > Subject: Re: [Histonet] Looking for detailed protocol to stain for PAS > in liver sections (paraffin) > > > Gayle, > Yes, glycogen. > Does it mean that if we fixed livers in paraformaldehyde prepared on > PBS > we > lost glycogen? What if we fixed the whole liver, not section on a slide? > One > cannot remove glycogen from the whole liver this way. Thank you for this > info, > Igor > > Dr. Igor O. Nasonkin > Research Fellow > National Institutes of Health/NEI > 9000 Rockville Pike, MSC 1864 > Bldg 10, Room 10B11 > Bethesda, MD 20892 > Tel: 301-443-7398 -work > 617-388-4104 -cell > Fax 301-480-1769 > email: nasonkini@mail.nih.gov > http://www.nei.nih.gov/intramural/nnrl.asp > > On 5/6/08 8:08 PM, "Gayle Callis" wrote: > >> You did not say what you are trying to see in the liver? Glycogen? Some >> other PAS positive tissue component? If so,aqueous formalin fixation >> will remove the glyocgen. An alcoholic fixative helps retain >> glycogen. >> >> Gayle M. Callis >> HTL/HT/MT(ASCP) >> Bozeman MT 59715 >> >> ----- Original Message ----- >> From: "Igor Nasonkin" >> To: >> Sent: Tuesday, May 06, 2008 5:24 PM >> Subject: [Histonet] Looking for detailed protocol to stain for PAS in >> liver sections (paraffin) >> >>> I am looking for a detailed protocol for PAS staining in liver >>> sections (paraffin-embedded). The 1st set of sections did not work, >>> and we had no >>> +control since we have not done it. These are 4-6wk mouse liver >>> +sections >>> embedded in paraffin; our lab is experienced in IHC on paraffin >>> sections so these were done right. But PAS staining did not work. >>> What could be main reasons? Any positive control we could use? Thank >>> you in advance, Igor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 17 Date: Thu, 8 May 2008 09:29:21 +1000 From: "Tony Henwood" Subject: RE: More on [Histonet] Looking for detailed protocol to stain for PASin liver sections (paraffin) To: "Gayle Callis" , "Histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" Sorry, I have tried it on methanol fixed frozen sections of liver and it works quite well Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: Gayle Callis [mailto:gayle.callis@bresnan.net] Sent: Thursday, 8 May 2008 9:24 AM To: Tony Henwood Subject: Re: More on [Histonet] Looking for detailed protocol to stain for PASin liver sections (paraffin) Tony, What a delightful variation and I bet it would work on tissue sections too. Gayle Callis ----- Original Message ----- From: "Tony Henwood" To: "Gayle Callis" ; "Histonet" Sent: Wednesday, May 07, 2008 5:17 PM Subject: RE: More on [Histonet] Looking for detailed protocol to stain for PASin liver sections (paraffin) I have found the following Modified PAS procedure especially useful for cytology smears that have been fixed in ethanol: Alcoholic PAS Stain Mucins and glycogen are water soluble. Over-rinsing slides, especially cytological smears, could result in excessive loss of these PAS substances. The following variant of the PAS stain performs the reactions in alcohol, thus decreasing the loss of these substances. Solutions: 1. Alcoholic Schiff reagent Basic Fuchsin 0.5g Ethanol 80ml Distilled Water 20ml Hydrochloric acid 1ml 2. Alcoholic Periodic acid Periodic acid 0.5g 95% ethanol 50ml Procedure: 1. Fix smears in 95% ethanol or use methanol fixed air-dried smears. 2. Place in alcoholic periodic acid 20min. 3. Wash in alcohol 4. Place in alcoholic Schiff's 20min. 5. Rinse slides in alcohol to remove excess dye. 6. Rinse slides in water. 7. Counterstain slides in Haematoxylin 2min. 8. Rinse slides in water. 9. Differentiate and Blue. 10. Dehydrate, clear and mount. References: Horobin and Kevill-Davis (1971) Stain Techn 46:53-58. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Thursday, 8 May 2008 2:06 AM To: Histonet Subject: Fw: More on [Histonet] Looking for detailed protocol to stain for PASin liver sections (paraffin) Dr. Nasonkin was staining for glycogen (per my question) and via private emailing, it was suggested he not use aqueous formalin or PFA fixation, but an alcoholic fixative to retain the glycogen in the liver, do diastase digestion to prove it is glyogen, and also process tissues - starting in higher (100%) alcohols to help retain glycogen. Suggested fixatives were Carnoy, Gendre and alcoholic formalin. If you have any other suggestions for him, please add to the list of to do's . Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT > ----- Original Message ----- > From: "Igor Nasonkin" > To: "Gayle Callis" > Cc: > Sent: Tuesday, May 06, 2008 6:35 PM > Subject: Re: [Histonet] Looking for detailed protocol to stain for PAS > in liver sections (paraffin) > > > Gayle, > Yes, glycogen. > Does it mean that if we fixed livers in paraformaldehyde prepared on > PBS we > lost glycogen? What if we fixed the whole liver, not section on a slide? > One > cannot remove glycogen from the whole liver this way. Thank you for this > info, > Igor > > Dr. Igor O. Nasonkin > Research Fellow > National Institutes of Health/NEI > 9000 Rockville Pike, MSC 1864 > Bldg 10, Room 10B11 > Bethesda, MD 20892 > Tel: 301-443-7398 -work > 617-388-4104 -cell > Fax 301-480-1769 > email: nasonkini@mail.nih.gov > http://www.nei.nih.gov/intramural/nnrl.asp > > On 5/6/08 8:08 PM, "Gayle Callis" wrote: > >> You did not say what you are trying to see in the liver? Glycogen? Some >> other PAS positive tissue component? If so,aqueous formalin fixation >> will remove the glyocgen. An alcoholic fixative helps retain >> glycogen. >> >> Gayle M. Callis >> HTL/HT/MT(ASCP) >> Bozeman MT 59715 >> >> ----- Original Message ----- >> From: "Igor Nasonkin" >> To: >> Sent: Tuesday, May 06, 2008 5:24 PM >> Subject: [Histonet] Looking for detailed protocol to stain for PAS in >> liver sections (paraffin) >> >>> I am looking for a detailed protocol for PAS staining in liver >>> sections (paraffin-embedded). The 1st set of sections did not work, >>> and we had no >>> +control since we have not done it. These are 4-6wk mouse liver >>> +sections >>> embedded in paraffin; our lab is experienced in IHC on paraffin >>> sections so these were done right. But PAS staining did not work. >>> What could be main reasons? Any positive control we could use? Thank >>> you in advance, Igor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 18 Date: Wed, 7 May 2008 17:11:11 -0700 (PDT) From: Akemi Allison-Tacha Subject: Re: [Histonet] Re; Decal Process From the Past To: HistoNet Server , Paul Bradbury Message-ID: <251486.25591.qm@web31304.mail.mud.yahoo.com> Content-Type: text/plain; charset=us-ascii Hi Paul, I appreciate your courteous reply to the older methodologies, but as I stated, this was just "throwing an additional tidbit into the mix". As you are most likely aware, histology in the past was not considered an exact science. Histotech's referred to formula's as recipes and the results for the most part, depended on the phase on the moon and the technical skill and knowledge of the individual. Most of the histotech's I ran a crossed in the 60's, 70's, 80's and even the 90's were OJT. There are histotech's that are still following method's that were passed on to them years and years ago and aren't sure why. This procedure was for larger bone specimens. We never put BM cores into RDO, that solution was much too harsh for such a fragile specimen. For BM cores, we used a gentle decal solution that had EDTA incorporated into it. It usually only took a half hour and we never left it longer than an hour. We rinsed the decalcified BM core in running tap water, then placed it on the processor. When I started doing technical support for a biotech company, I became aware of the "old recipes" and variations in adhering to set procedures. There were a number of histotech's that had NO idea why they were doing a procedure, or how each chemical step impacted the final result. These histotech's had no clue of what theory and practice was all about. It was a wonderful opportunity to try to help my fellow histologists in any way possible and gave me a opportunity to grow as well. A day does not go by that I don't grow and learn something new. Akemi Allison-Tacha, BS, HT(ASCP)HTL Client Services Manager PhenoPath laboratories 551 North 34th Street, Suite 100 Seattle, WA 98103-8675 Work: (206) 374-9000 ext 1053 E-Mail: akemiat3377@yahoo.com --- On Tue, 5/6/08, Paul Bradbury wrote: > From: Paul Bradbury > Subject: [Histonet] Re; RINSING IN TAP WATER AFTER DECAL > To: "HistoNet Server" > Date: Tuesday, May 6, 2008, 11:40 PM > With all due respect to Akemi, the practice of > "neutralizing" the > decalcifying acid by treating the tissue with saturated > sodium > bicarbonate is not a good idea. > In her e-mail she states that "The tissue fizzes a bit > ..." no surprise > there. Trying to retain good morphology in bone biopsy core > is difficult > enough without disrupting the tissues with bubbles of > carbon dioxide. A > bone core is usually only 2-3 mm in diameter, so washing > out the > decalcifying fluid will take only a few minutes. Once the > acid has been > removed, decalcification will stop. > Paul Bradbury > Kamloops, Canada > > ****************************************************************** > > > Akemi Allison-Tacha wrote: > >> Hi All, > >> > >> I am going to put an additional tidbit into the > mix. I was instructed many many moons ago, (back in 1974) > to 1st rinse off any residual RDO or decal solution with a > brief rinse in tap water and then place the decal specimens > into saturated sodium bicarbonate for 10 minutes to stop the > decal process, otherwise the specimen will continue to > decal. The tissue fizzes a bit, when this reaction ceases, > then rinse in running tap water for an additional 10 > minutes. > >> > >> Akemi Allison-Tacha, BS, HT(ASCP)HTL > >> Client Services Manager > >> PhenoPath laboratories > >> 551 North 34th Street, Suite 100 > >> Seattle, WA 98103-8675 > >> Work: (206) 374-9000 ext 1053 > >> E-Mail: akemiat3377@yahoo.com > >> > >> > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Wed, 7 May 2008 20:20:36 -0400 From: "jstaruk" Subject: RE: [Histonet] Too many Techs... To: "'Bonner, Janet'" , "'Anne van Binsbergen'" , "'barbara carter'" , , "'Histonet'" Message-ID: Content-Type: text/plain; charset="us-ascii" _____________________ Jim Staruk Mass Histology Service www.masshistology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonner, Janet Sent: Wednesday, May 07, 2008 1:06 PM To: Anne van Binsbergen; barbara carter; histonet-request@lists.utsouthwestern.edu; Histonet Subject: [Histonet] Too many Techs... 10,000cases? 3300 cases per tech per year, 100 cases per tech per day? At least 300 blocks min. per day per tech. CAP has guidelines for justifying positions. I would also have the techs (like they need something else to do) keep track of their time for a month. It sounds as if three techs would minimally handle your lab. Not to mention two months worth of vacation/sick time taken per year. Also - what is the boss seeing? Coffee breaks? Long lunch breaks? We have 22 Techs, three shifts, six days per week, 50,000 Surgical cases. It takes two techs worth just to cover time off per year. We do not attend procedures and we buy our reagents. Good luck. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Anne van Binsbergen Sent: Wed 5/7/2008 12:09 PM To: barbara carter; histonet-request@lists.utsouthwestern.edu; Histonet Subject: Re: [Histonet] Histology Coordinator Position in Kenosha Wisconsin i have a few questions for you if i may. you have 3 techs serving 3 paths and 10 000 a year apart from the basic routine histo operations, do your 3 techs - do any grossing - attend at renal biopsy collection - do frozen sections - take macro photos - do slide and block filing - source cases for sendout (consults/reviews) or do any archive searches - wash glassware, - do specimen discards, - make up formalin i am facing pressure from my (non-histo) boss who is comparing my lab and staff to 'other modern facilities' and he now tells me i need to cut my staff in half!! i need ammo and numbers from others to build my defence cheers Annie 2008/5/7 barbara carter : > > I am leaving my position as Coordinator at United Hospital System and am > moving out of state and trying to help them recruit a new coordinator to > take my place. > > There is a position open at United Hospital System at the Kenosha Campus > in Kenosha Wisconsin for a Histology Coordinator. The hospital receives > about 10,000 surgical specimen a year with 3 Pathologists and 3 Histologist. > > Anyone interested please contact Sue Wergin Laboratory Supervisor at > 262-656-5603. > > > > > _________________________________________________________________ > Windows Live SkyDrive lets you share files with faraway friends. > > http://www.windowslive.com/skydrive/overview.html?ocid=TXT_TAGLM_WL_Refr esh_ skydrive_052008_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne (van Binsbergen) Hope Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Wed, 7 May 2008 20:27:40 -0400 From: "Amos Brooks" Subject: [Histonet] Polymer IHC Detection To: histonet@lists.utsouthwestern.edu Message-ID: <582736990805071727n10f00e42w643fadfd7f7b8d0f@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi, Does anyone know of a good polymer immunohistochemical detection kit that labels antibodise raised in animals other than mouse or rabbit? I am looking for a goat or rat detection kit. I know I could use a mouse anti goat then a mouse secondary, but I'd like to simplify the process. Of course vendor responses are more than welcome. Thanks, Amos Brooks ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 54, Issue 9 *************************************** From leiker <@t> buffalo.edu Thu May 8 14:24:33 2008 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Thu May 8 14:24:51 2008 Subject: [Histonet] ERRATUM in Why use Calcium Chloride in Trypsin Antigen Retrieval? In-Reply-To: <4C54C915415D9C933626AEA2@bchwxp2702.ad.med.buffalo.edu> References: <4C54C915415D9C933626AEA2@bchwxp2702.ad.med.buffalo.edu> Message-ID: <8855FC0BD46FE5FF9D0C3671@bchwxp2702.ad.med.buffalo.edu> Sorry I meant FORMaldehyde-fixed, not paraformaldehyde.... --On Thursday, May 08, 2008 3:14 PM -0400 Merced Leiker wrote: > Does anyone know why CaCl2 is used in trypsin buffers for retrieval of > antigen in paraformaldehyde-fixed, paraffin-embedded tissue sections? Is > it really necessary? Can I just use a Trypsin-EDTA solution we already > have in the lab? > > Merced M Leiker > Research Technician II > 354 BRB (Lee Lab) / 140 Farber Hall (mail) > School of Medicine and Biomedical Sciences > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 > Ph: (716) 829-6033 > Fx: (716) 829-2725 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 BRB (Lee Lab) / 140 Farber Hall (mail) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 From kenneth.metzger <@t> aruplab.com Thu May 8 14:29:53 2008 From: kenneth.metzger <@t> aruplab.com (Metzger, Kenneth) Date: Thu May 8 14:29:19 2008 Subject: [Histonet] Bone Marrow Cores Message-ID: We recently have switched from Z-5 to formalin for fixing our bone marrow cores (Medical Director's request). We are now having issues with the cores staying on the slides during IHC. We have tried extra Sta-On and extra baking to avail. Any suggestions. Thanks, Ken Ken Metzger HTL(ASCP) Histology Supervisor ARUP Laboratories 500 Chipeta way Salt Lake City, UT 84108 801.583.2787 ext 3101 - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From mpence <@t> grhs.net Thu May 8 14:29:59 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Thu May 8 14:30:07 2008 Subject: [Histonet] pigment in bone marrow clot sections Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3822@IS-E2K3.grhs.net> I need to ask if anyone has seen this before? In our bone marrow clot section that we stain H&E, I am seeing black precipitate that looks like you were looking at anthracotic pigment. This is at the same level as the section on the slide. It is not debris it is only on the stained section (the rest of the glass looks clean) and we fix in 10% NBF. It is not zinc, like we use to see in B-5 fixed tissues. We only see it in very bloody specimens like bone marrows or blood clots. Has any one else see this or know what it is or how to get rid of it? Thanks in advance, Mike From anitaibsc <@t> aol.com Fri May 9 14:33:34 2008 From: anitaibsc <@t> aol.com (AnitaIBSC) Date: Thu May 8 14:32:30 2008 Subject: [Histonet] Epithelial specific antigen EP CAM Message-ID: <6e6a9080.712d.4cb7.bfe9.a20e4e6dab70@aol.com> Hello All, We have about 15 mgs of Epithelial specific antigen EP CAM, FITC labeled antibody, which has been implicated in stem cell research. For further information contact us at anitaibsc@aol.com. Our web site is not set up yet. Thanks, Anita Hingorani VP Marketing/Sales ImmunoBioScience Corp. 650-343-IBSC From liz <@t> premierlab.com Thu May 8 14:34:08 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu May 8 14:34:13 2008 Subject: [Histonet] pigment in bone marrow clot sections In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3822@IS-E2K3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838C017A3822@IS-E2K3.grhs.net> Message-ID: I would be its formalin pigment, you need to check the pH of the fixative. You can get rid of it check freida's book. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Thursday, May 08, 2008 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] pigment in bone marrow clot sections I need to ask if anyone has seen this before? In our bone marrow clot section that we stain H&E, I am seeing black precipitate that looks like you were looking at anthracotic pigment. This is at the same level as the section on the slide. It is not debris it is only on the stained section (the rest of the glass looks clean) and we fix in 10% NBF. It is not zinc, like we use to see in B-5 fixed tissues. We only see it in very bloody specimens like bone marrows or blood clots. Has any one else see this or know what it is or how to get rid of it? Thanks in advance, Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jlyoder <@t> mail2jason.com Thu May 8 14:35:09 2008 From: jlyoder <@t> mail2jason.com (jason yoder) Date: Thu May 8 14:35:24 2008 Subject: [Histonet] please remove me from list Message-ID: <07d701c8b142$a03e3b50$066a010a@mail2world.com>

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Unlimited Email Storage – POP3 – Calendar – SMS – Translator – Much More! From Erin.Martin <@t> ucsf.edu Thu May 8 14:37:11 2008 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Thu May 8 14:39:53 2008 Subject: [Histonet] Re: Bone flap from skull Message-ID: Holy cow!!! The other response about telling a court that some people on line told you how to handle it is bad bad bad. Let the pathologists deal with it - do not get involved! Run! A neurosurgeon just brought a bone flap from a patient and wants to replace it 3-4 days from now... Do any of you have suggestions or recommendations for storing the bone flap in the lab until he needs it again??? Need help fast! Thanks! From dellav <@t> musc.edu Thu May 8 15:07:39 2008 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Thu May 8 15:07:42 2008 Subject: [Histonet] RE: Bone Flap from Skull In-Reply-To: <4A3619571D9F6C4CB79C980E91DBE4E61FAC94@TNTRIEXEVS03.triadhospitals.net> References: <4A3619571D9F6C4CB79C980E91DBE4E61FAC94@TNTRIEXEVS03.triadhospitals.net> Message-ID: Hi Robert, Aside for the "run for your life" advice, I wanted to offer something perhaps a bit more useful. I had the same scenario arise at a previous facility. Tissues to be reimplanted should be handled by blood bank or another service that can ensure sterility and temperature of storage (chart recorder on freezer) etc. I am fairly certain that there are FDA requirements that must be adhered to for re-implantation. This is definitely NOT a pathology function. Typically they are looking for someone with an ultra-low freezer but that is not the proper way to handle these requests. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lott, Robert Sent: Thursday, May 08, 2008 12:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bone Flap from Skull A neurosurgeon just brought a bone flap from a patient and wants to replace it 3-4 days from now... Do any of you have suggestions or recommendations for storing the bone flap in the lab until he needs it again??? Need help fast! Thanks! Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology Trinity Medical Center / LabFirst 800 Montclair Road Birmingham, AL 35213 205-592-5388 205-592-5646 - fax robert.lott@triadhospitals.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kenneth.metzger <@t> aruplab.com Thu May 8 15:08:30 2008 From: kenneth.metzger <@t> aruplab.com (Metzger, Kenneth) Date: Thu May 8 15:07:56 2008 Subject: [Histonet] Update Bone Marrow Cores Message-ID: Just to clarify something I should have put in the first message...We are using plus-slides. Thanks Ken Metzger HTL(ASCP) Histology Supervisor ARUP Laboratories 500 Chipeta way Salt Lake City, UT 84108 801.583.2787 ext 3101 - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From nancy_schmitt <@t> pa-ucl.com Thu May 8 15:17:07 2008 From: nancy_schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Thu May 8 15:17:14 2008 Subject: [Histonet] cassette labeler/printer Message-ID: <9FC023A4AB52BB4D87DC6456081A822C087C87@mercury.pa-ucl.com> Hi histonetters I am looking for feedback on cassette labeler/printers. We are starting to check into this instrumentation and would appreciate any information you can give - good or bad! Are you interfaced with it? Do you wish you were? Are you glad you are not? We have Copath Plus DHT. Is the size of the printer an issue? Thanks in advance for your help Nancy Schmitt Histology Coordinator Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From Susan.Weber2 <@t> va.gov Thu May 8 15:36:18 2008 From: Susan.Weber2 <@t> va.gov (Weber, Susan (VHACLE)) Date: Thu May 8 15:36:24 2008 Subject: [Histonet] Re: Too many Techs... In-Reply-To: References: <5F31F38C96781A4FBE3196EBC22D47807F2621@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: <16C83872A53F4346AA9C3A18E3A3AAB903F76D9C@VHAV10MSGA1.v10.med.va.gov> Janet- At my former job, I was by myself for 5 years. We had 1 Pathologist, 1 Secretary (who also did general lab secretarial duties) and a part time diener for Autopsies. They finally gave in and I was able to add a part time tech who covered for me full time if I was on vacation or off. We had 3500 cases per year. Primarily orthopedic Frozen sections on synovial tissue for polys and lots of decals! (I cut the knees and hips on a Bone Band saw with a diamond blade). We also had a breast surgeon who was very much into Microcals and Frozen sections. I occasionally went to Radiology to assist during sectioning for specimen radiograph. For CT Radiology specimens, we did not have to go there for FNA's but, they did bring them down for a rapid Dif Quick type stain. We did all of our Special stains by hand (purchased reagents) and hand coverslipping of slides. We sent our IHC blocks out to a sister hospital in the system for staining. I did ALL the accessioning and registration of drop off specimens, non-gyn cytoprep, QC/QA/QI, and was also on the Code Orange (Hazmat) team and safety officer, AND when the Pathology secretary wasn't there I did her job as well. Not to mention the general maintenance and cleaning of the grossing area, processing/cutting/staining autopsy tissue also. Was I busy?!?! Volume doesn't necessarily make for busy technologists! Note: I did say former job?! Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne van Binsbergen Sent: Wednesday, May 07, 2008 11:52 PM To: Bonner, Janet Cc: histonet-request@lists.utsouthwestern.edu; Histonet; barbara carter Subject: [Histonet] Re: Too many Techs... no, we do way less than 10 000 a year my point is that in my opinion, no matter how LOW the workload may be, there are several basic areas which need to be covered if one has to attend to all the listed 'peripheral' services, as well as cover the basic daily routine histo (and be the cyto backup!!) i KNOW my staff are busy because i know exactly what it takes to cover all the areas they are involved in but the boss is a 'number cruncher' and he see things through 'hematology' eyes and all is compared to them histo is very different breaks are taken with a clock running next to a coffee cup - lunch is eaten on the trot samples are collected in OR by techs and they do all the reception/accessioning/sorting out mistakes etc very time consuming but not really measurable, particularly if one is running around like a headless chicken dealing with irate surgeons looking for results!! so i am trying to get a handle on all your labs out there, what you actually do, *apart from basic histo*, and how many of you there are to do it do you have dedicated admin staff do you have filing clerks and accession/data entry staff does your AP section have a secretary to deal with clinicians demanding path reports do the lab staff (techs) have to deal with phone queries for results any/all feedback appreciated Annie ps when doing stats for headcounts, is IHC classified as a sub-section apart with its own staff and back up or is it counted with routine histo? 2008/5/7 Bonner, Janet : > 10,000cases? 3300 cases per tech per year, 100 cases per tech per > day? At least 300 blocks min. per day per tech. > CAP has guidelines for justifying positions. I would also have the > techs (like they need something else to do) keep track of their time for a > month. It sounds as if three techs would minimally handle your lab. Not to > mention two months worth of vacation/sick time taken per year. > Also - what is the boss seeing? Coffee breaks? Long lunch breaks? > We have 22 Techs, three shifts, six days per week, 50,000 Surgical > cases. It takes two techs worth just to cover time off per year. We do not > attend procedures and we buy our reagents. > Good luck. > ------------------------------ > *From:* histonet-bounces@lists.utsouthwestern.edu on behalf of Anne van > Binsbergen > *Sent:* Wed 5/7/2008 12:09 PM > *To:* barbara carter; histonet-request@lists.utsouthwestern.edu; Histonet > *Subject:* Re: [Histonet] Histology Coordinator Position in Kenosha > Wisconsin > > i have a few questions for you if i may. > you have 3 techs serving 3 paths and 10 000 a year > apart from the basic routine histo operations, do your 3 techs > > - do any grossing > - attend at renal biopsy collection > - do frozen sections > - take macro photos > - do slide and block filing > - source cases for sendout (consults/reviews) or do any archive > searches > - wash glassware, > - do specimen discards, > - make up formalin > > i am facing pressure from my (non-histo) boss who is comparing my lab and > staff to 'other modern facilities' and he now tells me i need to cut my > staff in half!! > i need ammo and numbers from others to build my defence > cheers > Annie > > 2008/5/7 barbara carter : > > > > > I am leaving my position as Coordinator at United Hospital System and am > > moving out of state and trying to help them recruit a new coordinator to > > take my place. > > > > There is a position open at United Hospital System at the Kenosha Campus > > in Kenosha Wisconsin for a Histology Coordinator. The hospital receives > > about 10,000 surgical specimen a year with 3 Pathologists and 3 > Histologist. > > > > Anyone interested please contact Sue Wergin Laboratory Supervisor at > > 262-656-5603. > > > > > > > > > > _________________________________________________________________ > > Windows Live SkyDrive lets you share files with faraway friends. > > > > > http://www.windowslive.com/skydrive/overview.html?ocid=TXT_TAGLM_WL_Refr esh_skydrive_052008_______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Anne (van Binsbergen) Hope > Abu Dhabi > UAE > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ======================================================= > The information contained in this message may be privileged and/or confidential > and protected from disclosure. If the reader of this message is not the intended > recipient or an employee or agent responsible for delivering this message to the > intended recipient, you are hereby notified that any dissemination, distribution > or copying of this communication is strictly prohibited. If you have received this > communication in error, please notify the sender immediately by replying to this > message and deleting the material from any computer. > ======================================================= > > -- Anne (van Binsbergen) Hope Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Weber2 <@t> va.gov Thu May 8 15:39:41 2008 From: Susan.Weber2 <@t> va.gov (Weber, Susan (VHACLE)) Date: Thu May 8 15:39:50 2008 Subject: [Histonet] RE: Histonet Digest, Vol 54, Issue 9 In-Reply-To: <61A9977919846C479389493BAE2517CA01530941@MHSEXMBX1.multicare.org> References: <643C96FA2KK9638-01@MMS_multicare.org> <61A9977919846C479389493BAE2517CA01530941@MHSEXMBX1.multicare.org> Message-ID: <16C83872A53F4346AA9C3A18E3A3AAB903F76D9D@VHAV10MSGA1.v10.med.va.gov> Thank you for you information. The vendor (BBC Biochemical) contacted me and is sending out a sample to evaluate. Thanks to all who responded to my query! Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Spair Sent: Thursday, May 08, 2008 2:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 54, Issue 9 B plus is made by a company called BBC in Standwood, Washington. They have a web site you can visit. There are a few vendors that carry this product for them as well. I don't remember which ones off hand. However go to www.bbcus.com and check it out. John Spair, Manager Pathology Services LABORATORIES Northwest MultiCare Health System PO Box 5299, Tacoma WA 98415 Phone: 253-403-6090 Fax: 253-403-1357 Message: 2 Date: Wed, 7 May 2008 13:09:45 -0400 From: "Weber, Susan (VHACLE)" Subject: RE: [Histonet] B-plus question To: "Paul Bradbury" , "karen adams" , "HistoNet Server" Message-ID: <16C83872A53F4346AA9C3A18E3A3AAB903F76D96@VHAV10MSGA1.v10.med.va.gov> Content-Type: text/plain; charset="us-ascii" I may have missed this, but may I have the name of the Vendor you purchase your B-plus from. Thanks. Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 "MMS " made the following annotations. ------------------------------------------------------------------------ ------ NOTICE: This e-mail and the attachments hereto, if any, may contain privileged and/or confidential information. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, you are hereby notified that any examination, distribution or copying of this e-mail and the attachments hereto, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by email or telephone and permanently delete this e-mail and the attachments hereto, if any, and destroy any printout thereof. MultiCare Health System, Tacoma, WA 98415 (253) 403-1000. ======================================================================== ====== From godsgalnow <@t> aol.com Thu May 8 16:26:00 2008 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Thu May 8 16:26:14 2008 Subject: [Histonet] FISH probes In-Reply-To: <999031.60307.qm@web34605.mail.mud.yahoo.com> References: <999031.60307.qm@web34605.mail.mud.yahoo.com> Message-ID: <8CA7F693ECDDC25-688-8D4@webmail-db15.sysops.aol.com> What are you looking for? Roxanne -----Original Message----- From: Jordan Phillips To: histonet@lists.utsouthwestern.edu Sent: Tue, 6 May 2008 10:31 pm Subject: [Histonet] FISH probes My lab is in the process of begining FISH testing.? Can anyone help with what probes to order?? Thanks ____________________________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Thu May 8 18:01:23 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Thu May 8 18:01:24 2008 Subject: An option for handling [Histonet] Re: Bone flap from skull References: Message-ID: <001a01c8b15f$700f27b0$6501a8c0@DHXTS541> There are bone banks for this purpose, and should be able to advise laboratories how to handle the bone after collection. The bone bank itself might have to be involved. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT ----- Original Message ----- From: "Martin, Erin" To: "histonet" Sent: Thursday, May 08, 2008 1:37 PM Subject: [Histonet] Re: Bone flap from skull Holy cow!!! The other response about telling a court that some people on line told you how to handle it is bad bad bad. Let the pathologists deal with it - do not get involved! Run! A neurosurgeon just brought a bone flap from a patient and wants to replace it 3-4 days from now... Do any of you have suggestions or recommendations for storing the bone flap in the lab until he needs it again??? Need help fast! Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMyers1 <@t> aol.com Thu May 8 18:37:26 2008 From: JMyers1 <@t> aol.com (JMyers1@aol.com) Date: Thu May 8 18:37:33 2008 Subject: [Histonet] microwave pressure cookers Message-ID: Vikki: I think that what you're referring to is the NordicWare 'Tender-Cooker', which is placed into a microwave oven after loading with water and containers holding slides in a retrieval solution. The insert is not 'lab-certified' per se, but you certainly could validate a procedure using this combination of devices if you wished. This item can be purchased from Nordic Ware: _http://www.nordicware.com/store/products/detail/22688074-7C89-102A-B382-0002B3267AD7_ (http://www.nordicware.com/store/products/detail/22688074-7C89-102A-B382-0002B3267AD7) (or, for $10 less, from Amazon: _http://www.amazon.com/Nordicware-62104-Nordic-Tender-Cooker/dp/B0007LC55A_ (http://www.amazon.com/Nordicware-62104-Nordic-Tender-Cooker/dp/B0007LC55A) ). Good Luck, Joe Myers ------------------------------ Message: 10 Date: Thu, 8 May 2008 06:21:28 -0500 From: "Victoria Baker" Subject: [Histonet] microwave pressure cookers To: histonet _histonet@pathology.swmed.edu_ (mailto:histonet@pathology.swmed.edu) Hi Everyone, I'm trying to locate a 'lab certified' microwaveable pressure cooker. The last one I saw I thought might have been from BioCare or Biogenex. Does anyone know who carries them or what you are using for microwave pressure cookers? Thanks - Vikki **************Wondering what's for Dinner Tonight? Get new twists on family favorites at AOL Food. (http://food.aol.com/dinner-tonight?NCID=aolfod00030000000001) From jackdodo <@t> msn.com Fri May 9 00:47:16 2008 From: jackdodo <@t> msn.com (WAYNE HOLLAND) Date: Fri May 9 00:47:25 2008 Subject: [Histonet] I need your help Message-ID: Everyone, I have started a new job. I have many things that need fixed. I have a gross room that are using regular cassettes metal tops and they are wrapping all of the derm work in yes wet lens paper and leaving them on the countertop by the hoods for periods of up to 45 minutes. I know this is not good for obvious reasons. I need your comments asap to help make them understand, again for obvious reasons. I have been doing this for 28 years and I need other field associates to back me up. Most of these specimen are very small. HELP! From c.m.vanderloos <@t> amc.uva.nl Fri May 9 05:51:20 2008 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri May 9 05:51:56 2008 Subject: [Histonet] RE: Why use Calcium Chloride in Trypsin Antigen Message-ID: Dear Merced Leiker,If I remember correctly Ca-ions are necessary for trypsin to perform its enzymatic activity. The use of EDTA in your buffer is therefore perhaps not such a good idea. Just use a Tris-HCl buffer pH7.8. The addition of CaCl2 is only needed for some highly purified types of trypsin. Crude types of trypsin powder contain enough Ca and doesn't need the addition of CaCl2.Hope this helpsChrisChris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The NetherlandsDate: Thu, 08 May 2008 15:14:59 -0400 From: Merced Leiker Subject: [Histonet] Why use Calcium Chloride in Trypsin Antigen Retrieval? To: histonet@lists.utsouthwestern.edu Does anyone know why CaCl2 is used in trypsin buffers for retrieval of antigen in paraformaldehyde-fixed, paraffin-embedded tissue sections? Is it really necessary? Can I just use a Trypsin-EDTA solution we already have in the lab? Merced M Leiker Research Technician II 354 BRB (Lee Lab) / 140 Farber Hall (mail) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 From jnocito <@t> satx.rr.com Fri May 9 05:57:18 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri May 9 05:56:57 2008 Subject: [Histonet] Bone Flap from Skull References: Message-ID: <007001c8b1c3$7375faf0$0302a8c0@yourxhtr8hvc4p> has this doctor flipped his lid? I'd be blowing my top off. I hate when people loose their heads over a situation. I LOVE FRIDAYSSSSSSSSSSSSS JTT ----- Original Message ----- From: "Rivka Rachel" To: "Geoff McAuliffe" Cc: ; "Lott,Robert" Sent: Thursday, May 08, 2008 12:18 PM Subject: Re: [Histonet] Bone Flap from Skull > As a former neurosurgeon myself, I second that vote. > Rivka > > > On 5/8/08 1:16 PM, "Geoff McAuliffe" wrote: > >> You do not need help with this, you need to get far away from this. >> I can't believe your institution does not have a protocol for this! >> Are you really going to take recommendations from Histoneters who are >> not MDs? >> I can just see you testifying at the malpractice trail (after the >> infected/necrotic tissue causes serious >> illness or death) that someone on histonet told you it was OK to >> ........... >> Get my drift? >> This is obvious malpractice on the surgeon's part. Run like hell. >> >> Geoff >>> -----Original Message----- >>> From: histonet-bounces@lists.utsouthwestern.edu >>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lott, >>> Robert >>> Sent: Thursday, May 08, 2008 11:58 AM >>> To: histonet@lists.utsouthwestern.edu >>> Subject: [Histonet] Bone Flap from Skull >>> >>> >>> A neurosurgeon just brought a bone flap from a patient and wants to >>> replace it 3-4 days from now... >>> >>> Do any of you have suggestions or recommendations for storing the bone >>> flap in the lab until he needs it again??? >>> >>> >>> >>> Need help fast! >>> >>> >>> >>> Thanks! >>> >>> >>> >>> >>> >>> Robert L. Lott, HTL(ASCP) >>> >>> Manager, Anatomic Pathology >>> >>> Trinity Medical Center / LabFirst >>> >>> 800 Montclair Road >>> >>> Birmingham, AL 35213 >>> >>> 205-592-5388 >>> >>> 205-592-5646 - fax >>> >>> robert.lott@triadhospitals.com >>> >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >>> >> > > -- > Rivka A. Rachel, MD, PhD > Staff Scientist, National Eye Institute > Neurobiology, Neurodegeneration & Repair Laboratory > Bldg 10 Rm 10D43 > Tel: 301 443-4906 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Fri May 9 06:01:27 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri May 9 06:01:10 2008 Subject: [Histonet] Bone Marrow Cores References: Message-ID: <00a001c8b1c4$079b3e20$0302a8c0@yourxhtr8hvc4p> see if you can get a sample of B-Plus from BBC biochemical. The fixing results mimic the old B-5 mercury-laded fixative. We don't have any problems with cores staying on. The other option is to obtain positive charged slides. They are more expensive than regular slides, but how can you put a price on a potential law suit or have to re-do another bone marrow procedure? JTT ----- Original Message ----- From: "Metzger, Kenneth" To: Sent: Thursday, May 08, 2008 2:29 PM Subject: [Histonet] Bone Marrow Cores We recently have switched from Z-5 to formalin for fixing our bone marrow cores (Medical Director's request). We are now having issues with the cores staying on the slides during IHC. We have tried extra Sta-On and extra baking to avail. Any suggestions. Thanks, Ken Ken Metzger HTL(ASCP) Histology Supervisor ARUP Laboratories 500 Chipeta way Salt Lake City, UT 84108 801.583.2787 ext 3101 - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Weber2 <@t> va.gov Fri May 9 10:20:21 2008 From: Susan.Weber2 <@t> va.gov (Weber, Susan (VHACLE)) Date: Fri May 9 10:20:42 2008 Subject: [Histonet] Too many Techs... In-Reply-To: <44780C571F28624DBB446DE55C4D733A1FE5B6@EXCHANGEBE1.carle.com> References: <5F31F38C96781A4FBE3196EBC22D47807F2621@fhosxchmb006.ADVENTISTCORP.NET> <44780C571F28624DBB446DE55C4D733A1FE5B6@EXCHANGEBE1.carle.com> Message-ID: <16C83872A53F4346AA9C3A18E3A3AAB903F76DA2@VHAV10MSGA1.v10.med.va.gov> As I stated before, Volume does not mean someone is not busy, look at the other tasks (even if they are not "histotech" duties) I would suggest a time study where each individual records what is being done during the day. Don't forget the tasks which are not done on a daily basis, but are equally important and little tasks (recording QC on charts for example). If your techs go to Radiology - include the time they left until the time they return, not just the procedure time and remember that when a person leaves a department to do such tasks, the other techs must "fill in" for that missing person just as if they were off. I take exception to the fact that so many people determine how staffing should be by using numbers and not looking at the "big" picture, also by people who really do not work in a department telling others how to staff the job (pet peeve, sorry) and not taking into account that most of the tasks (especially in a lower volume lab) are NOT automated (as in Chemisty, Hematology etc). OK so I have some issues with this and you might say I have several "Pets" so I will step off my soapbox, take my blood pressure pills, be thankful I said "former job" and double check my IRA and 401K for the future use! Whew! TGIF all! Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles.Embrey Sent: Thursday, May 08, 2008 9:25 AM To: Bonner, Janet Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Too many Techs... Janet, I don't understand your math. 10,000 cases per year divided by 52 would be 192 cases a week divided between 3 techs would be 64 cases each tech per week and average 12.8 cases per tech per day. It doesn't sound too bad to me. 100 cases per tech per day (at 3 techs) would equal about 72,000 cases per year. Chuck -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonner, Janet Sent: Wednesday, May 07, 2008 12:06 PM To: Anne van Binsbergen; barbara carter; histonet-request@lists.utsouthwestern.edu; Histonet Subject: [Histonet] Too many Techs... 10,000cases? 3300 cases per tech per year, 100 cases per tech per day? At least 300 blocks min. per day per tech. CAP has guidelines for justifying positions. I would also have the techs (like they need something else to do) keep track of their time for a month. It sounds as if three techs would minimally handle your lab. Not to mention two months worth of vacation/sick time taken per year. Also - what is the boss seeing? Coffee breaks? Long lunch breaks? We have 22 Techs, three shifts, six days per week, 50,000 Surgical cases. It takes two techs worth just to cover time off per year. We do not attend procedures and we buy our reagents. Good luck. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Anne van Binsbergen Sent: Wed 5/7/2008 12:09 PM To: barbara carter; histonet-request@lists.utsouthwestern.edu; Histonet Subject: Re: [Histonet] Histology Coordinator Position in Kenosha Wisconsin i have a few questions for you if i may. you have 3 techs serving 3 paths and 10 000 a year apart from the basic routine histo operations, do your 3 techs - do any grossing - attend at renal biopsy collection - do frozen sections - take macro photos - do slide and block filing - source cases for sendout (consults/reviews) or do any archive searches - wash glassware, - do specimen discards, - make up formalin i am facing pressure from my (non-histo) boss who is comparing my lab and staff to 'other modern facilities' and he now tells me i need to cut my staff in half!! i need ammo and numbers from others to build my defence cheers Annie 2008/5/7 barbara carter : > > I am leaving my position as Coordinator at United Hospital System and am > moving out of state and trying to help them recruit a new coordinator to > take my place. > > There is a position open at United Hospital System at the Kenosha Campus > in Kenosha Wisconsin for a Histology Coordinator. The hospital receives > about 10,000 surgical specimen a year with 3 Pathologists and 3 Histologist. > > Anyone interested please contact Sue Wergin Laboratory Supervisor at > 262-656-5603. > > > > > _________________________________________________________________ > Windows Live SkyDrive lets you share files with faraway friends. > > http://www.windowslive.com/skydrive/overview.html?ocid=TXT_TAGLM_WL_Refr esh_skydrive_052008_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne (van Binsbergen) Hope Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From plucas <@t> biopath.org Fri May 9 10:39:54 2008 From: plucas <@t> biopath.org (Paula Lucas) Date: Fri May 9 10:40:00 2008 Subject: [Histonet] Hematoxylin too light Message-ID: <20080509153954.CF4FC27C5@courageux.cnchost.com> Hello, My pathologist here likes the nuclear staining very dark. Does anyone have such a pathologist who likes a dark nucleus and if so, would you mind suggesting the H&E protocol and brand of hematoxylin used? We have the Leica autostainer and I'm using the Richard Allan 7211 with the times of 2.5 minutes in the Hematoxylin 7211 and 1.5 minutes in Eosin. I use the Clarifier 1 for 30 seconds and the Bluing reagent for 1 minute. I increased the time to 5 minutes in the 7211, but it really didn't make any changes. I've been using this method for the past several years, but this past year, the pathologist has been complaining about the stain being too pale. I would appreciate any suggestions. Thanks. Paula Lucas Lab Manager Bio-Path Medical Group From mary.helie <@t> yale.edu Fri May 9 11:00:13 2008 From: mary.helie <@t> yale.edu (Mary Helie) Date: Fri May 9 11:00:22 2008 Subject: [Histonet] Re: Histonet Digest, Vol 54, Issue 11 In-Reply-To: <200805081934.m48JYmxL005210@mr3.its.yale.edu> References: <200805081934.m48JYmxL005210@mr3.its.yale.edu> Message-ID: <4824750D.8010901@yale.edu> CaCl makes the trypsin "work" chelating (?spelling) agent from what I recall. You can buy ready to use tablets from Sigma T 7168 just dissolve in 1cc DI water 40' 20 min. histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Bone Flap from Skull (Sebree Linda A.) > 2. Re: Bone Flap from Skull (Rene J Buesa) > 3. Re: Bone Flap from Skull (Geoff McAuliffe) > 4. Re: Bone Flap from Skull (Rivka Rachel) > 5. California licensure (Martin, Erin) > 6. (no subject) (Kathleen Boozer) > 7. CAP Her2Neu regs (Satterfield, Marirose) > 8. Why use Calcium Chloride in Trypsin Antigen Retrieval? > (Merced Leiker) > 9. RE: Polymer IHC Detection (Wynn, Carmen) > 10. ERRATUM in Why use Calcium Chloride in Trypsin Antigen > Retrieval? (Merced Leiker) > 11. Bone Marrow Cores (Metzger, Kenneth) > 12. pigment in bone marrow clot sections (Mike Pence) > 13. Epithelial specific antigen EP CAM (AnitaIBSC) > 14. RE: pigment in bone marrow clot sections (Liz Chlipala) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 8 May 2008 12:02:34 -0500 > From: "Sebree Linda A." > Subject: RE: [Histonet] Bone Flap from Skull > To: "Lott, Robert" , > > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > Just off the top of my head (no pun intended) I would think an organ > preservation solution might be reasonable assuming that your institution > is involved in transplantation. > > Linda Sebree, HT(ASCP) > University of Wisconsin Hospital & Clinics > IHC/ISH Laboratory > A4/204-3224 > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > FAX: (608)262-7174 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lott, > Robert > Sent: Thursday, May 08, 2008 11:58 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Bone Flap from Skull > > > A neurosurgeon just brought a bone flap from a patient and wants to > replace it 3-4 days from now... > > Do any of you have suggestions or recommendations for storing the bone > flap in the lab until he needs it again??? > > > > Need help fast! > > > > Thanks! > > > > > > Robert L. Lott, HTL(ASCP) > > Manager, Anatomic Pathology > > Trinity Medical Center / LabFirst > > 800 Montclair Road > > Birmingham, AL 35213 > > 205-592-5388 > > 205-592-5646 - fax > > robert.lott@triadhospitals.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 2 > Date: Thu, 8 May 2008 10:15:14 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Bone Flap from Skull > To: "Lott, Robert" , > histonet@lists.utsouthwestern.edu > Message-ID: <160753.70968.qm@web65705.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Freeze it at -80?C > Ren? J. > > "Lott, Robert" wrote: > A neurosurgeon just brought a bone flap from a patient and wants to > replace it 3-4 days from now... > > Do any of you have suggestions or recommendations for storing the bone > flap in the lab until he needs it again??? > > > > Need help fast! > > > > Thanks! > > > > > > Robert L. Lott, HTL(ASCP) > > Manager, Anatomic Pathology > > Trinity Medical Center / LabFirst > > 800 Montclair Road > > Birmingham, AL 35213 > > 205-592-5388 > > 205-592-5646 - fax > > robert.lott@triadhospitals.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. > > ------------------------------ > > Message: 3 > Date: Thu, 08 May 2008 13:16:51 -0400 > From: Geoff McAuliffe > Subject: Re: [Histonet] Bone Flap from Skull > Cc: histonet@lists.utsouthwestern.edu, "Lott, Robert" > > Message-ID: <48233583.3050809@umdnj.edu> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > You do not need help with this, you need to get far away from this. > I can't believe your institution does not have a protocol for this! > Are you really going to take recommendations from Histoneters who are > not MDs? > I can just see you testifying at the malpractice trail (after the > infected/necrotic tissue causes serious > illness or death) that someone on histonet told you it was OK to ........... > Get my drift? > This is obvious malpractice on the surgeon's part. Run like hell. > > Geoff > >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lott, >> Robert >> Sent: Thursday, May 08, 2008 11:58 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Bone Flap from Skull >> >> >> A neurosurgeon just brought a bone flap from a patient and wants to >> replace it 3-4 days from now... >> >> Do any of you have suggestions or recommendations for storing the bone >> flap in the lab until he needs it again??? >> >> >> >> Need help fast! >> >> >> >> Thanks! >> >> >> >> >> >> Robert L. Lott, HTL(ASCP) >> >> Manager, Anatomic Pathology >> >> Trinity Medical Center / LabFirst >> >> 800 Montclair Road >> >> Birmingham, AL 35213 >> >> 205-592-5388 >> >> 205-592-5646 - fax >> >> robert.lott@triadhospitals.com >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> > > > From rjbuesa <@t> yahoo.com Fri May 9 11:11:55 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri May 9 11:11:58 2008 Subject: [Histonet] Hematoxylin too light In-Reply-To: <20080509153954.CF4FC27C5@courageux.cnchost.com> Message-ID: <418086.35900.qm@web65703.mail.ac4.yahoo.com> Just give more staining time to the one you are using, differentiate less and make sure that bluing is complete (perhaps use a lithium carbonate solution as a bluing agent. Do NOT use ammonia, because it could cause the section to peel-off). Ren? J. Paula Lucas wrote: --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From mary.helie <@t> yale.edu Fri May 9 11:15:28 2008 From: mary.helie <@t> yale.edu (Mary Helie) Date: Fri May 9 11:15:42 2008 Subject: [Histonet] Re: Histonet Digest, Vol 54, Issue 11 In-Reply-To: <200805081934.m48JYmxL005210@mr3.its.yale.edu> References: <200805081934.m48JYmxL005210@mr3.its.yale.edu> Message-ID: <482478A0.5050109@yale.edu> Before I get swamped I do not know if EDTA would or would not work I know it chelates as well- I was just familiar with CaCl2. histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Bone Flap from Skull (Sebree Linda A.) > 2. Re: Bone Flap from Skull (Rene J Buesa) > 3. Re: Bone Flap from Skull (Geoff McAuliffe) > 4. Re: Bone Flap from Skull (Rivka Rachel) > 5. California licensure (Martin, Erin) > 6. (no subject) (Kathleen Boozer) > 7. CAP Her2Neu regs (Satterfield, Marirose) > 8. Why use Calcium Chloride in Trypsin Antigen Retrieval? > (Merced Leiker) > 9. RE: Polymer IHC Detection (Wynn, Carmen) > 10. ERRATUM in Why use Calcium Chloride in Trypsin Antigen > Retrieval? (Merced Leiker) > 11. Bone Marrow Cores (Metzger, Kenneth) > 12. pigment in bone marrow clot sections (Mike Pence) > 13. Epithelial specific antigen EP CAM (AnitaIBSC) > 14. RE: pigment in bone marrow clot sections (Liz Chlipala) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 8 May 2008 12:02:34 -0500 > From: "Sebree Linda A." > Subject: RE: [Histonet] Bone Flap from Skull > To: "Lott, Robert" , > > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > Just off the top of my head (no pun intended) I would think an organ > preservation solution might be reasonable assuming that your institution > is involved in transplantation. > > Linda Sebree, HT(ASCP) > University of Wisconsin Hospital & Clinics > IHC/ISH Laboratory > A4/204-3224 > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > FAX: (608)262-7174 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lott, > Robert > Sent: Thursday, May 08, 2008 11:58 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Bone Flap from Skull > > > A neurosurgeon just brought a bone flap from a patient and wants to > replace it 3-4 days from now... > > Do any of you have suggestions or recommendations for storing the bone > flap in the lab until he needs it again??? > > > > Need help fast! > > > > Thanks! > > > > > > Robert L. Lott, HTL(ASCP) > > Manager, Anatomic Pathology > > Trinity Medical Center / LabFirst > > 800 Montclair Road > > Birmingham, AL 35213 > > 205-592-5388 > > 205-592-5646 - fax > > robert.lott@triadhospitals.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 2 > Date: Thu, 8 May 2008 10:15:14 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Bone Flap from Skull > To: "Lott, Robert" , > histonet@lists.utsouthwestern.edu > Message-ID: <160753.70968.qm@web65705.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Freeze it at -80?C > Ren? J. > > "Lott, Robert" wrote: > A neurosurgeon just brought a bone flap from a patient and wants to > replace it 3-4 days from now... > > Do any of you have suggestions or recommendations for storing the bone > flap in the lab until he needs it again??? > > > > Need help fast! > > > > Thanks! > > > > > > Robert L. Lott, HTL(ASCP) > > Manager, Anatomic Pathology > > Trinity Medical Center / LabFirst > > 800 Montclair Road > > Birmingham, AL 35213 > > 205-592-5388 > > 205-592-5646 - fax > > robert.lott@triadhospitals.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. > > ------------------------------ > > Message: 3 > Date: Thu, 08 May 2008 13:16:51 -0400 > From: Geoff McAuliffe > Subject: Re: [Histonet] Bone Flap from Skull > Cc: histonet@lists.utsouthwestern.edu, "Lott, Robert" > > Message-ID: <48233583.3050809@umdnj.edu> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > You do not need help with this, you need to get far away from this. > I can't believe your institution does not have a protocol for this! > Are you really going to take recommendations from Histoneters who are > not MDs? > I can just see you testifying at the malpractice trail (after the > infected/necrotic tissue causes serious > illness or death) that someone on histonet told you it was OK to ........... > Get my drift? > This is obvious malpractice on the surgeon's part. Run like hell. > > Geoff > >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lott, >> Robert >> Sent: Thursday, May 08, 2008 11:58 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Bone Flap from Skull >> >> >> A neurosurgeon just brought a bone flap from a patient and wants to >> replace it 3-4 days from now... >> >> Do any of you have suggestions or recommendations for storing the bone >> flap in the lab until he needs it again??? >> >> >> >> Need help fast! >> >> >> >> Thanks! >> >> >> >> >> >> Robert L. Lott, HTL(ASCP) >> >> Manager, Anatomic Pathology >> >> Trinity Medical Center / LabFirst >> >> 800 Montclair Road >> >> Birmingham, AL 35213 >> >> 205-592-5388 >> >> 205-592-5646 - fax >> >> robert.lott@triadhospitals.com >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> > > > From PMonfils <@t> Lifespan.org Fri May 9 11:38:11 2008 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri May 9 11:38:20 2008 Subject: [Histonet] Hematoxylin too light In-Reply-To: <20080509153954.CF4FC27C5@courageux.cnchost.com> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273D77@LSRIEXCH1.lsmaster.lifespan.org> I don't know what Hematoxylin 7211 is, but a good quality Gill III formula applied for 5 minutes and blued in lithium carbonate solution will produce nuclei about as dark as you can get them with an aluminum-mordanted hematoxylin. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Paula Lucas > Reply To: Paula Lucas > Sent: Friday, May 9, 2008 11:39 AM > To: histonet@pathology.swmed.edu > Subject: [Histonet] Hematoxylin too light > > Hello, > > My pathologist here likes the nuclear staining very dark. > > Does anyone have such a pathologist who likes a dark nucleus and if so, would you mind suggesting the H&E protocol and brand of hematoxylin used? > > We have the Leica autostainer and I'm using the Richard Allan 7211 with the times of 2.5 minutes in the Hematoxylin 7211 and 1.5 minutes in Eosin. I use the Clarifier 1 for 30 seconds and the Bluing reagent for 1 minute. I increased the time to 5 minutes in the 7211, but it really didn't make any changes. I've been using this method for the past several years, but this past year, the pathologist has been complaining about the stain being too pale. > > I would appreciate any suggestions. Thanks. > Paula Lucas > Lab Manager > Bio-Path Medical Group > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JMahoney <@t> alegent.org Fri May 9 12:30:45 2008 From: JMahoney <@t> alegent.org (Mahoney,Janice A) Date: Fri May 9 12:31:01 2008 Subject: [Histonet] Bone Flap from Skull In-Reply-To: <007001c8b1c3$7375faf0$0302a8c0@yourxhtr8hvc4p> References: <007001c8b1c3$7375faf0$0302a8c0@yourxhtr8hvc4p> Message-ID: <346E5878979BA54FB4B0BFD6AD93B9B9B01F623355@EXCHMBC1.ad.ah.local> Joe, I'm impressed, did you come up with those puns right off the top of your head? Jan -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, May 09, 2008 5:57 AM To: Rivka Rachel; Geoff McAuliffe Cc: histonet@lists.utsouthwestern.edu; Lott, Robert Subject: Re: [Histonet] Bone Flap from Skull has this doctor flipped his lid? I'd be blowing my top off. I hate when people loose their heads over a situation. I LOVE FRIDAYSSSSSSSSSSSSS JTT ----- Original Message ----- From: "Rivka Rachel" To: "Geoff McAuliffe" Cc: ; "Lott,Robert" Sent: Thursday, May 08, 2008 12:18 PM Subject: Re: [Histonet] Bone Flap from Skull > As a former neurosurgeon myself, I second that vote. > Rivka > > > On 5/8/08 1:16 PM, "Geoff McAuliffe" wrote: > >> You do not need help with this, you need to get far away from this. >> I can't believe your institution does not have a protocol for this! >> Are you really going to take recommendations from Histoneters who are >> not MDs? >> I can just see you testifying at the malpractice trail (after the >> infected/necrotic tissue causes serious >> illness or death) that someone on histonet told you it was OK to >> ........... >> Get my drift? >> This is obvious malpractice on the surgeon's part. Run like hell. >> >> Geoff >>> -----Original Message----- >>> From: histonet-bounces@lists.utsouthwestern.edu >>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lott, >>> Robert >>> Sent: Thursday, May 08, 2008 11:58 AM >>> To: histonet@lists.utsouthwestern.edu >>> Subject: [Histonet] Bone Flap from Skull >>> >>> >>> A neurosurgeon just brought a bone flap from a patient and wants to >>> replace it 3-4 days from now... >>> >>> Do any of you have suggestions or recommendations for storing the bone >>> flap in the lab until he needs it again??? >>> >>> >>> >>> Need help fast! >>> >>> >>> >>> Thanks! >>> >>> >>> >>> >>> >>> Robert L. Lott, HTL(ASCP) >>> >>> Manager, Anatomic Pathology >>> >>> Trinity Medical Center / LabFirst >>> >>> 800 Montclair Road >>> >>> Birmingham, AL 35213 >>> >>> 205-592-5388 >>> >>> 205-592-5646 - fax >>> >>> robert.lott@triadhospitals.com >>> >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >>> >> > > -- > Rivka A. Rachel, MD, PhD > Staff Scientist, National Eye Institute > Neurobiology, Neurodegeneration & Repair Laboratory > Bldg 10 Rm 10D43 > Tel: 301 443-4906 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From leiker <@t> buffalo.edu Fri May 9 12:46:27 2008 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Fri May 9 12:46:31 2008 Subject: [Histonet] What do microtomes retail at? In-Reply-To: <346E5878979BA54FB4B0BFD6AD93B9B9B01F623355@EXCHMBC1.ad.ah.local> References: <007001c8b1c3$7375faf0$0302a8c0@yourxhtr8hvc4p> <346E5878979BA54FB4B0BFD6AD93B9B9B01F623355@EXCHMBC1.ad.ah.local> Message-ID: Anyone know what a basic microtome retails at? I'm looking at purchasing a refurbished Leica/Reichert 2030 for $3000 and just wanted to know what a similar one costs new. Thanks! Merced M Leiker Research Technician II 354 BRB (Lee Lab) / 140 Farber Hall (mail) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 From bdelescavage <@t> cellnetix.com Fri May 9 13:20:16 2008 From: bdelescavage <@t> cellnetix.com (Beth Delescavage) Date: Fri May 9 13:20:41 2008 Subject: [Histonet] CellNetix Job Opportunities Message-ID: Hello Everyone~ The laboratory I am working for is looking to hire two team members: The first being a laboratory assistant duties will include accessioning, assisting the grossing technician, load and unloading the tissue processor, performing quality control on the tissue processor, recycling reagents, embedding tissue in paraffin, storage and disposal of tissue. The candidate would rotate through these assigned work areas and perform additional tasks assigned by the supervisor. The second being an HT/HTL or HT/HTL eligible individual to join our growing team; the duties will included embedding paraffin, loading and unloading the tissue processors, cutting paraffin blocks for H&E, special stains, and IHC slides, IHC staining, special stains, and other routine Histology duties. The candidate would rotate through these assigned work areas and perform additional tasks assigned by the supervisor, and have 1-2 years of Histology work experience. To find out more about the laboratory positions and the wonderful city of Seattle, contact William Lynch (Histology Supervisor) at 206-215-5952 or e-mail at wlynch@cellnetix.com Also check us out on the web at www.cellnetix.com Beth Delescavage, BS, HTL (ASCP) QIHC CellNetix Laboratories Seattle, WA DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From gayle.callis <@t> bresnan.net Fri May 9 13:25:49 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Fri May 9 13:25:51 2008 Subject: [Histonet] Re: Histonet Digest, Vol 54, Issue 11 References: <200805081934.m48JYmxL005210@mr3.its.yale.edu> <482478A0.5050109@yale.edu> Message-ID: <006d01c8b202$1b4c8ee0$6501a8c0@DHXTS541> If you have EDTA and also have calcium present in the solution, the EDTA is going to chelate the calcium, removing it. If calcium is needed in a solution, I don't think I would want to have EDTA in there with it. We have use crude trypsin for digestions to avoid having to add calcium to make the trypsin active (per Chris van der Loos reply). Our enzyme digestion with crude trypsin worked fine. Gayle M. Callis HTL/HT/MT(ASCP) ----- Original Message ----- From: "Mary Helie" To: Sent: Friday, May 09, 2008 10:15 AM Subject: [Histonet] Re: Histonet Digest, Vol 54, Issue 11 Before I get swamped I do not know if EDTA would or would not work I know it chelates as well- I was just familiar with CaCl2. histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Bone Flap from Skull (Sebree Linda A.) > 2. Re: Bone Flap from Skull (Rene J Buesa) > 3. Re: Bone Flap from Skull (Geoff McAuliffe) > 4. Re: Bone Flap from Skull (Rivka Rachel) > 5. California licensure (Martin, Erin) > 6. (no subject) (Kathleen Boozer) > 7. CAP Her2Neu regs (Satterfield, Marirose) > 8. Why use Calcium Chloride in Trypsin Antigen Retrieval? > (Merced Leiker) > 9. RE: Polymer IHC Detection (Wynn, Carmen) > 10. ERRATUM in Why use Calcium Chloride in Trypsin Antigen > Retrieval? (Merced Leiker) > 11. Bone Marrow Cores (Metzger, Kenneth) > 12. pigment in bone marrow clot sections (Mike Pence) > 13. Epithelial specific antigen EP CAM (AnitaIBSC) > 14. RE: pigment in bone marrow clot sections (Liz Chlipala) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 8 May 2008 12:02:34 -0500 > From: "Sebree Linda A." > Subject: RE: [Histonet] Bone Flap from Skull > To: "Lott, Robert" , > > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > Just off the top of my head (no pun intended) I would think an organ > preservation solution might be reasonable assuming that your institution > is involved in transplantation. > > Linda Sebree, HT(ASCP) > University of Wisconsin Hospital & Clinics > IHC/ISH Laboratory > A4/204-3224 > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > FAX: (608)262-7174 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lott, > Robert > Sent: Thursday, May 08, 2008 11:58 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Bone Flap from Skull > > > A neurosurgeon just brought a bone flap from a patient and wants to > replace it 3-4 days from now... > > Do any of you have suggestions or recommendations for storing the bone > flap in the lab until he needs it again??? > > > Need help fast! > > > Thanks! > > > > Robert L. Lott, HTL(ASCP) > > Manager, Anatomic Pathology > > Trinity Medical Center / LabFirst > > 800 Montclair Road > > Birmingham, AL 35213 > > 205-592-5388 > > 205-592-5646 - fax > > robert.lott@triadhospitals.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 2 > Date: Thu, 8 May 2008 10:15:14 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Bone Flap from Skull > To: "Lott, Robert" , > histonet@lists.utsouthwestern.edu > Message-ID: <160753.70968.qm@web65705.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Freeze it at -80?C > Ren? J. > > "Lott, Robert" wrote: > A neurosurgeon just brought a bone flap from a patient and wants to > replace it 3-4 days from now... > > Do any of you have suggestions or recommendations for storing the bone > flap in the lab until he needs it again??? > > > > Need help fast! > > > > Thanks! > > > > > > Robert L. Lott, HTL(ASCP) > > Manager, Anatomic Pathology > > Trinity Medical Center / LabFirst > > 800 Montclair Road > > Birmingham, AL 35213 > > 205-592-5388 > > 205-592-5646 - fax > > robert.lott@triadhospitals.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > --------------------------------- > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it > now. > > ------------------------------ > > Message: 3 > Date: Thu, 08 May 2008 13:16:51 -0400 > From: Geoff McAuliffe > Subject: Re: [Histonet] Bone Flap from Skull > Cc: histonet@lists.utsouthwestern.edu, "Lott, Robert" > > Message-ID: <48233583.3050809@umdnj.edu> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > You do not need help with this, you need to get far away from this. > I can't believe your institution does not have a protocol for this! > Are you really going to take recommendations from Histoneters who are not > MDs? > I can just see you testifying at the malpractice trail (after the > infected/necrotic tissue causes serious > illness or death) that someone on histonet told you it was OK to > ........... > Get my drift? > This is obvious malpractice on the surgeon's part. Run like hell. > > Geoff > >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lott, >> Robert >> Sent: Thursday, May 08, 2008 11:58 AM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Bone Flap from Skull >> >> >> A neurosurgeon just brought a bone flap from a patient and wants to >> replace it 3-4 days from now... >> >> Do any of you have suggestions or recommendations for storing the bone >> flap in the lab until he needs it again??? >> >> >> Need help fast! >> >> >> Thanks! >> >> >> >> Robert L. Lott, HTL(ASCP) >> >> Manager, Anatomic Pathology >> >> Trinity Medical Center / LabFirst >> >> 800 Montclair Road >> >> Birmingham, AL 35213 >> >> 205-592-5388 >> >> 205-592-5646 - fax >> >> robert.lott@triadhospitals.com >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri May 9 15:15:18 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri May 9 15:15:23 2008 Subject: [Histonet] CellNetix Job Opportunities In-Reply-To: Message-ID: <135134.49988.qm@web65713.mail.ac4.yahoo.com> Embedding? An assistant? Ren? J. Beth Delescavage wrote: Hello Everyone~ The laboratory I am working for is looking to hire two team members: The first being a laboratory assistant duties will include accessioning, assisting the grossing technician, load and unloading the tissue processor, performing quality control on the tissue processor, recycling reagents, embedding tissue in paraffin, storage and disposal of tissue. The candidate would rotate through these assigned work areas and perform additional tasks assigned by the supervisor. The second being an HT/HTL or HT/HTL eligible individual to join our growing team; the duties will included embedding paraffin, loading and unloading the tissue processors, cutting paraffin blocks for H&E, special stains, and IHC slides, IHC staining, special stains, and other routine Histology duties. The candidate would rotate through these assigned work areas and perform additional tasks assigned by the supervisor, and have 1-2 years of Histology work experience. To find out more about the laboratory positions and the wonderful city of Seattle, contact William Lynch (Histology Supervisor) at 206-215-5952 or e-mail at wlynch@cellnetix.com Also check us out on the web at www.cellnetix.com Beth Delescavage, BS, HTL (ASCP) QIHC CellNetix Laboratories Seattle, WA DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From bdelescavage <@t> cellnetix.com Fri May 9 15:20:35 2008 From: bdelescavage <@t> cellnetix.com (Beth Delescavage) Date: Fri May 9 15:21:03 2008 Subject: [Histonet] (no subject) Message-ID: Hi Rene~ I know what you must be thinking, and for the most part I agree with you. We have several lab assistants that want to be HT and have started their training with embedding. Don't worry the patient is always first and I would not let just anyone embed tissue, they will be trained first. Thanks Beth Beth Delescavage, BS, HTL (ASCP) QIHC CellNetix Laboratories DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From CrochiereSteve <@t> aol.com Fri May 9 18:15:56 2008 From: CrochiereSteve <@t> aol.com (CrochiereSteve@aol.com) Date: Fri May 9 18:16:04 2008 Subject: [Histonet] record slides? Message-ID: How many slides have you cut in an 8 hour day? One of my techs today cut 658 prostate bx slides in 7 hours. Just curious. steve **************Wondering what's for Dinner Tonight? Get new twists on family favorites at AOL Food. (http://food.aol.com/dinner-tonight?NCID=aolfod00030000000001) From freckles9660 <@t> yahoo.com Fri May 9 18:17:40 2008 From: freckles9660 <@t> yahoo.com (Karla Arrington) Date: Fri May 9 18:17:45 2008 Subject: [Histonet] IHC Background Staining Message-ID: <390591.1467.qm@web32505.mail.mud.yahoo.com> I do Immuno's by hand and I am getting background DAB precipitate on the inside circle of my sections. What is causing this and how do I get rid of it? ____________________________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ From jnocito <@t> satx.rr.com Fri May 9 18:38:03 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri May 9 18:37:23 2008 Subject: [Histonet] Bone Flap from Skull References: <007001c8b1c3$7375faf0$0302a8c0@yourxhtr8hvc4p> <346E5878979BA54FB4B0BFD6AD93B9B9B01F623355@EXCHMBC1.ad.ah.local> Message-ID: <005501c8b22d$b9d07ce0$0302a8c0@yourxhtr8hvc4p> yep. And I didn't blow my lid either ----- Original Message ----- From: "Mahoney,Janice A" To: "'Joe Nocito'" ; "Rivka Rachel" ; "Geoff McAuliffe" Cc: ; "Lott, Robert" Sent: Friday, May 09, 2008 12:30 PM Subject: RE: [Histonet] Bone Flap from Skull Joe, I'm impressed, did you come up with those puns right off the top of your head? Jan -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, May 09, 2008 5:57 AM To: Rivka Rachel; Geoff McAuliffe Cc: histonet@lists.utsouthwestern.edu; Lott, Robert Subject: Re: [Histonet] Bone Flap from Skull has this doctor flipped his lid? I'd be blowing my top off. I hate when people loose their heads over a situation. I LOVE FRIDAYSSSSSSSSSSSSS JTT ----- Original Message ----- From: "Rivka Rachel" To: "Geoff McAuliffe" Cc: ; "Lott,Robert" Sent: Thursday, May 08, 2008 12:18 PM Subject: Re: [Histonet] Bone Flap from Skull > As a former neurosurgeon myself, I second that vote. > Rivka > > > On 5/8/08 1:16 PM, "Geoff McAuliffe" wrote: > >> You do not need help with this, you need to get far away from this. >> I can't believe your institution does not have a protocol for this! >> Are you really going to take recommendations from Histoneters who are >> not MDs? >> I can just see you testifying at the malpractice trail (after the >> infected/necrotic tissue causes serious >> illness or death) that someone on histonet told you it was OK to >> ........... >> Get my drift? >> This is obvious malpractice on the surgeon's part. Run like hell. >> >> Geoff >>> -----Original Message----- >>> From: histonet-bounces@lists.utsouthwestern.edu >>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lott, >>> Robert >>> Sent: Thursday, May 08, 2008 11:58 AM >>> To: histonet@lists.utsouthwestern.edu >>> Subject: [Histonet] Bone Flap from Skull >>> >>> >>> A neurosurgeon just brought a bone flap from a patient and wants to >>> replace it 3-4 days from now... >>> >>> Do any of you have suggestions or recommendations for storing the bone >>> flap in the lab until he needs it again??? >>> >>> >>> >>> Need help fast! >>> >>> >>> >>> Thanks! >>> >>> >>> >>> >>> >>> Robert L. Lott, HTL(ASCP) >>> >>> Manager, Anatomic Pathology >>> >>> Trinity Medical Center / LabFirst >>> >>> 800 Montclair Road >>> >>> Birmingham, AL 35213 >>> >>> 205-592-5388 >>> >>> 205-592-5646 - fax >>> >>> robert.lott@triadhospitals.com >>> >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >>> >> > > -- > Rivka A. Rachel, MD, PhD > Staff Scientist, National Eye Institute > Neurobiology, Neurodegeneration & Repair Laboratory > Bldg 10 Rm 10D43 > Tel: 301 443-4906 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From ccrowder <@t> vetmed.lsu.edu Fri May 9 20:59:33 2008 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Fri May 9 21:02:27 2008 Subject: [Histonet] Louisiana Society Meeting in June Message-ID: Hello Histonetters, The Louisiana Society for Histotechnology is pleased to announce the 25th Annual Symposium/Convention "Histotechnology in Acadiana" will be held June 6 & 7, 2008. The meeting is located in Lafayette, LA at the Hotel Acadiana, 1801 W. Pinhook Road. The LSH block of rooms will be held until May 28th. For reservations call 337-233-8120 or 1-800-887-7371 and mention you are with the LSH group. Do to relocation of many of our members we would ask everyone who would like to receive a brochure/membership form in the mail or by fax, to please contact me via email vanmetmj@pbrc.edu [mailto:vanmetmj@pbrc.edu], 225-603-0953 or Hal Holloway at 225-769-6546. Walk-ins are always welcome! In addition, if you know of someone in your lab that would care to attend, simply make a copy of your registration form for them. The LSH would also like to extend the invitation to our fellow technologists and pathologists from surrounding states. We encourage everyone to attend in order to build our networking potential, earn those valuableCEU'sand enjoy some warm Cajun hospitality. We are very excited to have the well-known Forensic Anthropologist,Mary Manhein,as our keynote speaker. Her workshop, "The Bone Lady, Life as a Forensic Anthropologist", will be very educationaland include attendee participation. In addition, we will have a variety of topicspresentedby experienced speakers that promises to benefit everyone. The attendees will have access to several scientific vendor exhibits during the entire symposium. I have listed the workshops below and encourage y?all to come on over to Lafayette! 2008 LSH State Meeting Workshops: WS#1 - "The Bone Lady, Life as a ForensicAnthropologist? WS#2 - Histology ?Nuts and Bolts? ? Back to Basics WS#3 - CSI, Case Study Immunos WS#4 - Routine BoneHistotechniquesfor Clinical, Veterinary and Research Laboratories WS#5 - Laboratory Safety: Chemicals and Blood Borne Pathogens Thank you, Tina Van Meter Montina J. Van Meter, HT Lab Manager Autonomic Neuroscience Pennington Biomedical Research Center 6400 Perkins Rd. Baton Rouge, LA 70791 225-763-2622 From jstaruk <@t> masshistology.com Fri May 9 21:09:18 2008 From: jstaruk <@t> masshistology.com (jstaruk) Date: Fri May 9 21:09:36 2008 Subject: [Histonet] record slides? In-Reply-To: Message-ID: Steve, That's only 94 slides per hour. Were these serial sections off the same block or did the tech face each block and take one section per block? Were the slides pre-labeled? We prepare (among other things) teaching sets and one tech can prepare that many serial sections per hour (one section per slide on pre-labeled slides). We need specifics here. Jim _____________________ Jim Staruk Mass Histology Service www.masshistology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of CrochiereSteve@aol.com Sent: Friday, May 09, 2008 7:16 PM To: histonet@pathology.swmed.edu Subject: [Histonet] record slides? How many slides have you cut in an 8 hour day? One of my techs today cut 658 prostate bx slides in 7 hours. Just curious. steve **************Wondering what's for Dinner Tonight? Get new twists on family favorites at AOL Food. (http://food.aol.com/dinner-tonight?NCID=aolfod00030000000001) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Sat May 10 01:31:12 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat May 10 01:31:19 2008 Subject: AW: [Histonet] record slides? In-Reply-To: Message-ID: The first thought coming to my mind: poor girl/boy cutting the whole day. I like to cut, but I'am also glad to be ready after max. 3 hours (because of smaller workload, four techs cutting at the same time). Our prostate "menue": 2x HE ? 4 cuts, 1xIHC ? 4 cuts; 10 blocks per patient, without prelabelling; sliding microtome, 20-25 min In the mean we do 60-70 slides (with 1-4 cuts) per hour, without prelabelling, with walking in the staining room, with telephone, .... In my opinion it's a big difference, if one cuts at high speed for a few hours or has to do it the whole shift with the same concentration and quality. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von CrochiereSteve@aol.com Gesendet: Samstag, 10. Mai 2008 01:16 An: histonet@pathology.swmed.edu Betreff: [Histonet] record slides? How many slides have you cut in an 8 hour day? One of my techs today cut 658 prostate bx slides in 7 hours. Just curious. steve **************Wondering what's for Dinner Tonight? Get new twists on family favorites at AOL Food. (http://food.aol.com/dinner-tonight?NCID=aolfod00030000000001) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sat May 10 10:25:06 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat May 10 10:25:10 2008 Subject: [Histonet] IHC Background Staining In-Reply-To: <390591.1467.qm@web32505.mail.mud.yahoo.com> Message-ID: <564239.24626.qm@web65713.mail.ac4.yahoo.com> There could be so many causes that it would better for you if you check HistoNet archives. This subject has been the subject of many discussions in the past. Ren? J. Karla Arrington wrote: I do Immuno's by hand and I am getting background DAB precipitate on the inside circle of my sections. What is causing this and how do I get rid of it? ____________________________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From gayle.callis <@t> bresnan.net Sat May 10 10:37:47 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Sat May 10 10:37:48 2008 Subject: [Histonet] IHC Background Staining References: <390591.1467.qm@web32505.mail.mud.yahoo.com> Message-ID: <001701c8b2b3$cc055210$6501a8c0@DHXTS541> Adding detergent to all buffers and diluents helps, even before the DAB step. This prevents ionic interaction of chromogen to glass slide surface, 0.05% Tween 20. Someone can recommend concentration for Triton X-100. We found that drawing a circle around a section was more difficult to blot from (the circular edge). We now draw lines straight across, above and below the section, so blotting is done from a tipped slide and from the corner of the "square" after adequate rinsing. Other problems we encountered: Kits that were older, near expiration Some kits themselves gave more problems so we switched vendors OR microfiltered the DAB before application (which we disliked doing). Overdevelopment of the chromogen so the ppt is too heavy. We now monitor all chromogen development with a microscope (the positive control). This is easy to do with manual methods, and taught to me by a master of IHC, Dr. Chris van der Loos. Monitoring allows you to determine an approximate time for DAB development, duly noted for that antibody and future use. Good luck on solving the problem Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 . ----- Original Message ----- From: "Karla Arrington" To: Sent: Friday, May 09, 2008 5:17 PM Subject: [Histonet] IHC Background Staining >I do Immuno's by hand and I am getting background DAB precipitate on the >inside circle of my sections. > What is causing this and how do I get rid of it? > > > > ____________________________________________________________________________________ > Be a better friend, newshound, and > know-it-all with Yahoo! Mobile. Try it now. > http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From renafail <@t> bellsouth.net Sat May 10 11:21:40 2008 From: renafail <@t> bellsouth.net (Rena Fail) Date: Sat May 10 11:21:52 2008 Subject: [Histonet] IHC Background Staining In-Reply-To: <390591.1467.qm@web32505.mail.mud.yahoo.com> Message-ID: <000401c8b2b9$eeaa6f20$0301a8c0@RENAD4YK9B8ABE> The most common error made hand staining and the easiest to resolve is inadequate washing between steps. Make sure reagent is thoroughly drained from the slide and rinse with copious amts of buffer before proceeding to the next step Rena -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Karla Arrington Sent: Friday, May 09, 2008 7:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Background Staining I do Immuno's by hand and I am getting background DAB precipitate on the inside circle of my sections. What is causing this and how do I get rid of it? ________________________________________________________________________ ____________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Sat May 10 11:49:16 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Sat May 10 11:49:22 2008 Subject: [Histonet] Re: Bone Flap from Skull Message-ID: >>A neurosurgeon just brought a bone flap from a patient and wants to replace it 3-4 days from now... Do any of you have suggestions or recommendations for storing the bone flap in the lab until he needs it again???<< This is a problem that needs to be dumped on the pathologist's desk immediately. This is a "bone bank" problem - somebody up there suddenly decides that the pathology department is responsible for storing bone to be grafted into other patients by orthopedic surgeons - no testing for infectious agents, no records kept. Pathology departments are not equipped to handle sterile materials, nor to maintain patient records other than pathology reports. A conference between pathology, neurosurgery, and orthopedics is needed, perhaps urgently. Bob Richmond Samurai Pathologist Knoxville TN From amosbrooks <@t> gmail.com Sat May 10 17:14:17 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sat May 10 17:14:20 2008 Subject: [Histonet] record slides? Message-ID: <582736990805101514h42232700r67d3d51c57a4cb4@mail.gmail.com> WOW! Although I don't have such data to compare for you. I have to say that is really impressive. It averages out to 1.5 blocks per min. While it seems do-able, I think at that point I'd have to ask them to slow up a bit as it really would worry me when it comes to getting the right tissue on the right slides. If there were a mistake (s)he would have cut an aweful lot of blocks before realizing there was an error. Commendable, but scarey. Perhaps give him (her) wrist weights for a handicap :-) Amos Brooks Message: 7 Date: Fri, 9 May 2008 19:15:56 EDT From: CrochiereSteve@aol.com Subject: [Histonet] record slides? To: histonet@pathology.swmed.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" How many slides have you cut in an 8 hour day? One of my techs today cut 658 prostate bx slides in 7 hours. Just curious. steve From jackdodo <@t> msn.com Sat May 10 19:31:22 2008 From: jackdodo <@t> msn.com (WAYNE HOLLAND) Date: Sat May 10 19:31:30 2008 Subject: FW: RE: [Histonet] I need more ammunition Message-ID: I need more ammunition, please!! >From: WAYNE HOLLAND >Sent: Fri 5/9/2008 1:47 AM >To: Histonet@lists.utsouthwestern.edu >Subject: [Histonet] I need your help > > >Everyone, I have started a new job. I have many things that need fixed. I >have a gross room that are using regular cassettes metal tops and they are >wrapping all of the derm work in yes wet lens paper and leaving them on the >countertop by the hoods for periods of up to 45 minutes. I know this is not >good for obvious reasons. I need your comments asap to help make them >understand, again for obvious reasons. I have been doing this for 28 years >and I need other field associates to back me up. Most of these specimen are >very small. HELP! > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >NOTICE OF CONFIDENTIALITY >This electronic message, including attachments, is for the sole use of the >named recipient and may contain confidential or privileged information >protected by New York State, and Federal regulations. Any unauthorized >review, use, disclosure, copying or distribution is strictly prohibited. If >you are not the intended recipient or have received this communication in >error please contact the sender or email.security@bassett.org and destroy >all copies of the original message. Thank you. From gu.lang <@t> gmx.at Sun May 11 01:53:42 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sun May 11 01:53:53 2008 Subject: AW: RE: [Histonet] I need more ammunition In-Reply-To: Message-ID: <9D9A69851DA34EE08C0304AB3FB0E9C8@dielangs.at> How would your optimal handling look like? Tell us your "obvious reasons" -as a training for the discussions with your coworkers. Who is the person to change his/her opinion? What would/could happen, if the situation would'nt be changed? Is it a concern of the "CAP-thing?" Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von WAYNE HOLLAND Gesendet: Sonntag, 11. Mai 2008 02:31 An: Histonet@lists.utsouthwestern.edu Betreff: FW: RE: [Histonet] I need more ammunition I need more ammunition, please!! >From: WAYNE HOLLAND >Sent: Fri 5/9/2008 1:47 AM >To: Histonet@lists.utsouthwestern.edu >Subject: [Histonet] I need your help > > >Everyone, I have started a new job. I have many things that need fixed. I >have a gross room that are using regular cassettes metal tops and they are >wrapping all of the derm work in yes wet lens paper and leaving them on the >countertop by the hoods for periods of up to 45 minutes. I know this is not >good for obvious reasons. I need your comments asap to help make them >understand, again for obvious reasons. I have been doing this for 28 years >and I need other field associates to back me up. Most of these specimen are >very small. HELP! > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >NOTICE OF CONFIDENTIALITY >This electronic message, including attachments, is for the sole use of the >named recipient and may contain confidential or privileged information >protected by New York State, and Federal regulations. Any unauthorized >review, use, disclosure, copying or distribution is strictly prohibited. If >you are not the intended recipient or have received this communication in >error please contact the sender or email.security@bassett.org and destroy >all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Sun May 11 09:05:45 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sun May 11 09:05:39 2008 Subject: [Histonet] I need more ammunition References: Message-ID: <003001c8b370$1bfd18e0$0302a8c0@yourxhtr8hvc4p> Wayne, we perform almost 80 derm cases per day. We use blue sponges and not lens paper. The blue sponges make embedding so much faster. Often, the lens paper sticks together and makes it a nightmare to embed. Leaving any type of tissue on the counter is borderline malpractice. Not only does the tissue dry out, making processing and cutting difficult, it reeks havoc with immunohistochemistry. You make get no staining, weak staining or so much background staining you won't be able to read the slides. Where are you working? Nothing against you, but I want to make sure none of my family and friends are close by and pathology Joe Nocito BS, PA, HT(ASCP)QIHC San Antonio, TX ----- Original Message ----- From: "WAYNE HOLLAND" To: Sent: Saturday, May 10, 2008 7:31 PM Subject: FW: RE: [Histonet] I need more ammunition >I need more ammunition, please!! > >>From: WAYNE HOLLAND >>Sent: Fri 5/9/2008 1:47 AM >>To: Histonet@lists.utsouthwestern.edu >>Subject: [Histonet] I need your help >> >> >>Everyone, I have started a new job. I have many things that need fixed. I >>have a gross room that are using regular cassettes metal tops and they are >>wrapping all of the derm work in yes wet lens paper and leaving them on >>the countertop by the hoods for periods of up to 45 minutes. I know this >>is not good for obvious reasons. I need your comments asap to help make >>them understand, again for obvious reasons. I have been doing this for 28 >>years and I need other field associates to back me up. Most of these >>specimen are very small. HELP! >> >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >>NOTICE OF CONFIDENTIALITY >>This electronic message, including attachments, is for the sole use of the >>named recipient and may contain confidential or privileged information >>protected by New York State, and Federal regulations. Any unauthorized >>review, use, disclosure, copying or distribution is strictly prohibited. >>If you are not the intended recipient or have received this communication >>in error please contact the sender or email.security@bassett.org and >>destroy all copies of the original message. Thank you. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sun May 11 09:24:06 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun May 11 09:24:11 2008 Subject: FW: RE: [Histonet] I need more ammunition In-Reply-To: Message-ID: <40785.76974.qm@web65709.mail.ac4.yahoo.com> What you need is to explain to whomever is doing that to stop doing it. I don't think that you need anybody to support your words. You have to stand alone and explain WHY it is wrong and do NOT admit the continuation of such a bad practice. That practice has to stop regardless of how many years it has been done. Stand up for what is right. Ren? J. WAYNE HOLLAND wrote: --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From Rcartun <@t> harthosp.org Sun May 11 10:08:25 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Sun May 11 10:08:51 2008 Subject: [Histonet] IHC for TFE3 Message-ID: <4826D3A9020000770000C805@gwmail4.harthosp.org> Happy "Mother's Day" to all the moms out there! Does anyone know of a commercially-available antibody to the transcription factor "TFE3" used in the immunohistochemical evaluation of renal tumors? Thank you, Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From Barry.R.Rittman <@t> uth.tmc.edu Sun May 11 11:06:28 2008 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Sun May 11 11:06:33 2008 Subject: [Histonet] I need more ammunition In-Reply-To: <003001c8b370$1bfd18e0$0302a8c0@yourxhtr8hvc4p> References: <003001c8b370$1bfd18e0$0302a8c0@yourxhtr8hvc4p> Message-ID: Wayne I am not in your situation but I understand the problems when you are in a new job in histology. In many jobs the way in which things are done is because no one has guided the individuals in the job or have not adequately trained them. Once a practice has been in operation for some time it becomes the standard for that lab. I am assuming that you are in charge of this lab? Might I humbly suggest that you get individuals concerned together and ask them in a non confrontational manner why jobs are carried out in a certain manner. Perhaps this could be done in a casual setting over coffee. The suggest that there are better ways and show them these. Sometimes people will resist such changes and then you may have to insist or get your pathologist's backing. I have always found that it helps to put things down on paper with pros and cons of each method or practice. If there is, as Joe suggests a danger of poor or false negatives then you have a powerful argument both from point of view of costs (especially of immunological agents), of delays in having slides ready for the pathologist and (often the most convincing) the possibility legal consequences. One problem in all labs is that people need to feel that they have some say in the way their jobs are done. However while input is critical, it is important that when it comes down to it that the person in charge makes a decision and that the work is then done that way. Hope things work out for you. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Sunday, May 11, 2008 9:06 AM To: WAYNE HOLLAND; Histonet@lists.utsouthwestern.edu Subject: Re: RE: [Histonet] I need more ammunition Wayne, we perform almost 80 derm cases per day. We use blue sponges and not lens paper. The blue sponges make embedding so much faster. Often, the lens paper sticks together and makes it a nightmare to embed. Leaving any type of tissue on the counter is borderline malpractice. Not only does the tissue dry out, making processing and cutting difficult, it reeks havoc with immunohistochemistry. You make get no staining, weak staining or so much background staining you won't be able to read the slides. Where are you working? Nothing against you, but I want to make sure none of my family and friends are close by and pathology Joe Nocito BS, PA, HT(ASCP)QIHC San Antonio, TX ----- Original Message ----- From: "WAYNE HOLLAND" To: Sent: Saturday, May 10, 2008 7:31 PM Subject: FW: RE: [Histonet] I need more ammunition >I need more ammunition, please!! > >>From: WAYNE HOLLAND >>Sent: Fri 5/9/2008 1:47 AM >>To: Histonet@lists.utsouthwestern.edu >>Subject: [Histonet] I need your help >> >> >>Everyone, I have started a new job. I have many things that need fixed. I >>have a gross room that are using regular cassettes metal tops and they are >>wrapping all of the derm work in yes wet lens paper and leaving them on >>the countertop by the hoods for periods of up to 45 minutes. I know this >>is not good for obvious reasons. I need your comments asap to help make >>them understand, again for obvious reasons. I have been doing this for 28 >>years and I need other field associates to back me up. Most of these >>specimen are very small. HELP! >> >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >>NOTICE OF CONFIDENTIALITY >>This electronic message, including attachments, is for the sole use of the >>named recipient and may contain confidential or privileged information >>protected by New York State, and Federal regulations. Any unauthorized >>review, use, disclosure, copying or distribution is strictly prohibited. >>If you are not the intended recipient or have received this communication >>in error please contact the sender or email.security@bassett.org and >>destroy all copies of the original message. Thank you. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sun May 11 13:02:26 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun May 11 13:02:31 2008 Subject: [Histonet] I need more ammunition In-Reply-To: Message-ID: <893745.76665.qm@web65707.mail.ac4.yahoo.com> Did I miss the word "patient" or wasn't it in there? Ren? J. "Rittman, Barry R" wrote: Wayne I am not in your situation but I understand the problems when you are in a new job in histology. In many jobs the way in which things are done is because no one has guided the individuals in the job or have not adequately trained them. Once a practice has been in operation for some time it becomes the standard for that lab. I am assuming that you are in charge of this lab? Might I humbly suggest that you get individuals concerned together and ask them in a non confrontational manner why jobs are carried out in a certain manner. Perhaps this could be done in a casual setting over coffee. The suggest that there are better ways and show them these. Sometimes people will resist such changes and then you may have to insist or get your pathologist's backing. I have always found that it helps to put things down on paper with pros and cons of each method or practice. If there is, as Joe suggests a danger of poor or false negatives then you have a powerful argument both from point of view of costs (especially of immunological agents), of delays in having slides ready for the pathologist and (often the most convincing) the possibility legal consequences. One problem in all labs is that people need to feel that they have some say in the way their jobs are done. However while input is critical, it is important that when it comes down to it that the person in charge makes a decision and that the work is then done that way. Hope things work out for you. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Sunday, May 11, 2008 9:06 AM To: WAYNE HOLLAND; Histonet@lists.utsouthwestern.edu Subject: Re: RE: [Histonet] I need more ammunition Wayne, we perform almost 80 derm cases per day. We use blue sponges and not lens paper. The blue sponges make embedding so much faster. Often, the lens paper sticks together and makes it a nightmare to embed. Leaving any type of tissue on the counter is borderline malpractice. Not only does the tissue dry out, making processing and cutting difficult, it reeks havoc with immunohistochemistry. You make get no staining, weak staining or so much background staining you won't be able to read the slides. Where are you working? Nothing against you, but I want to make sure none of my family and friends are close by and pathology Joe Nocito BS, PA, HT(ASCP)QIHC San Antonio, TX ----- Original Message ----- From: "WAYNE HOLLAND" To: Sent: Saturday, May 10, 2008 7:31 PM Subject: FW: RE: [Histonet] I need more ammunition >I need more ammunition, please!! > >>From: WAYNE HOLLAND >>Sent: Fri 5/9/2008 1:47 AM >>To: Histonet@lists.utsouthwestern.edu >>Subject: [Histonet] I need your help >> >> >>Everyone, I have started a new job. I have many things that need fixed. I >>have a gross room that are using regular cassettes metal tops and they are >>wrapping all of the derm work in yes wet lens paper and leaving them on >>the countertop by the hoods for periods of up to 45 minutes. I know this >>is not good for obvious reasons. I need your comments asap to help make >>them understand, again for obvious reasons. I have been doing this for 28 >>years and I need other field associates to back me up. Most of these >>specimen are very small. HELP! >> >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >>NOTICE OF CONFIDENTIALITY >>This electronic message, including attachments, is for the sole use of the >>named recipient and may contain confidential or privileged information >>protected by New York State, and Federal regulations. Any unauthorized >>review, use, disclosure, copying or distribution is strictly prohibited. >>If you are not the intended recipient or have received this communication >>in error please contact the sender or email.security@bassett.org and >>destroy all copies of the original message. Thank you. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From jackdodo <@t> msn.com Sun May 11 13:10:34 2008 From: jackdodo <@t> msn.com (WAYNE HOLLAND) Date: Sun May 11 13:10:39 2008 Subject: [Histonet] I need more ammunition In-Reply-To: <893745.76665.qm@web65707.mail.ac4.yahoo.com> Message-ID: Oh, I am sure that is and was a consideration. I thank you all so very much for the additional comments, it helps me build a solid case. A mind changed against its will, is a mind unchanged still. This will help change their minds and way of thinking. >From: Rene J Buesa >To: "Rittman, Barry R" >,Histonet@lists.utsouthwestern.edu >Subject: RE: RE: [Histonet] I need more ammunition >Date: Sun, 11 May 2008 11:02:26 -0700 (PDT) > >Did I miss the word "patient" or wasn't it in there? >René J. > >"Rittman, Barry R" wrote: Wayne >I am not in your situation but I understand the problems when you are in >a new job in histology. >In many jobs the way in which things are done is because no one has >guided the individuals in the job or have not adequately trained them. >Once a practice has been in operation for some time it becomes the >standard for that lab. >I am assuming that you are in charge of this lab? >Might I humbly suggest that you get individuals concerned together and >ask them in a non confrontational manner why jobs are carried out in a >certain manner. Perhaps this could be done in a casual setting over >coffee. >The suggest that there are better ways and show them these. >Sometimes people will resist such changes and then you may have to >insist or get your pathologist's backing. >I have always found that it helps to put things down on paper with pros >and cons of each method or practice. If there is, as Joe suggests a >danger of poor or false negatives then you have a powerful argument both >from point of view of costs (especially of immunological agents), of >delays in having slides ready for the pathologist and (often the most >convincing) the possibility legal consequences. >One problem in all labs is that people need to feel that they have some >say in the way their jobs are done. However while input is critical, it >is important that when it comes down to it that the person in charge >makes a decision and that the work is then done that way. >Hope things work out for you. >Barry > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe >Nocito >Sent: Sunday, May 11, 2008 9:06 AM >To: WAYNE HOLLAND; Histonet@lists.utsouthwestern.edu >Subject: Re: RE: [Histonet] I need more ammunition > >Wayne, >we perform almost 80 derm cases per day. We use blue sponges and not >lens >paper. The blue sponges make embedding so much faster. Often, the lens >paper >sticks together and makes it a nightmare to embed. Leaving any type of >tissue on the counter is borderline malpractice. Not only does the >tissue >dry out, making processing and cutting difficult, it reeks havoc with >immunohistochemistry. You make get no staining, weak staining or so much > >background staining you won't be able to read the slides. >Where are you working? Nothing against you, but I want to make sure none >of >my family and friends are close by and pathology > >Joe Nocito BS, PA, HT(ASCP)QIHC >San Antonio, TX > > >----- Original Message ----- >From: "WAYNE HOLLAND" >To: >Sent: Saturday, May 10, 2008 7:31 PM >Subject: FW: RE: [Histonet] I need more ammunition > > > >I need more ammunition, please!! > > > >>From: WAYNE HOLLAND > >>Sent: Fri 5/9/2008 1:47 AM > >>To: Histonet@lists.utsouthwestern.edu > >>Subject: [Histonet] I need your help > >> > >> > >>Everyone, I have started a new job. I have many things that need >fixed. I > >>have a gross room that are using regular cassettes metal tops and they >are > >>wrapping all of the derm work in yes wet lens paper and leaving them >on > >>the countertop by the hoods for periods of up to 45 minutes. I know >this > >>is not good for obvious reasons. I need your comments asap to help >make > >>them understand, again for obvious reasons. I have been doing this for >28 > >>years and I need other field associates to back me up. Most of these > >>specimen are very small. HELP! > >> > >> > >> > >>_______________________________________________ > >>Histonet mailing list > >>Histonet@lists.utsouthwestern.edu > >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > >> > >>NOTICE OF CONFIDENTIALITY > >>This electronic message, including attachments, is for the sole use of >the > >>named recipient and may contain confidential or privileged information > > >>protected by New York State, and Federal regulations. Any unauthorized > > >>review, use, disclosure, copying or distribution is strictly >prohibited. > >>If you are not the intended recipient or have received this >communication > >>in error please contact the sender or email.security@bassett.org and > >>destroy all copies of the original message. Thank you. > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >--------------------------------- >Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it >now. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rick.Garnhart <@t> memorialhealthsystem.com Sun May 11 17:30:58 2008 From: Rick.Garnhart <@t> memorialhealthsystem.com (Rick.Garnhart@memorialhealthsystem.com) Date: Sun May 11 17:33:05 2008 Subject: [Histonet] Rick Garnhart/Histology/MEMHOSPCS is out of the office. Message-ID: I will be out of the office starting 05/11/2008 and will not return until 05/27/2008. I will respond to your message when I return. From akemiat3377 <@t> yahoo.com Sun May 11 18:16:28 2008 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Sun May 11 18:16:32 2008 Subject: [Histonet] Chuck Churukian turns 80 today Message-ID: <923957.21683.qm@web31308.mail.mud.yahoo.com> Hi All, I just wanted to share with all of you this special day in history for Chuck Churukian. Please join me in wishing Chuck a fabulous 80th birthday. I just spoke with him and he is having a wonderful day. He has several of his family members visiting and they went to the lilac festival. Jules Elias called him to express his best wishes and it made his day. Chuck is still working eight hours a week in the special stains lab at University of Rochester and is still involved with the Biological Stains Commission. He did however say that he is phasing out his involvement in presenting and attending histology meetings. We will miss his significant dedication and contribution to our field. Chuck said that the past ten years have been extremely rewarding. Especially with all of the awards he has received. If any of you want to express your wishes, reply to this e-mail and I will forward it on to him. Thank you, Akemi Akemi Allison-Tacha, BS, HT(ASCP)HTL Client Services Manager PhenoPath laboratories 551 North 34th Street, Suite 100 Seattle, WA 98103-8675 Work: (206) 374-9000 ext 1053 E-Mail: akemiat3377@yahoo.com From AnthonyH <@t> chw.edu.au Sun May 11 18:20:53 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun May 11 18:21:05 2008 Subject: [Histonet] Chuck Churukian turns 80 today In-Reply-To: <923957.21683.qm@web31308.mail.mud.yahoo.com> Message-ID: Chuck, Happy birthday. We in Australia have always appreciated your work and several of your modified techniques are still used in our lab. Great techniques that will definitely stand the test of time. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Monday, 12 May 2008 9:16 AM To: histo net Subject: [Histonet] Chuck Churukian turns 80 today Hi All, I just wanted to share with all of you this special day in history for Chuck Churukian. Please join me in wishing Chuck a fabulous 80th birthday. I just spoke with him and he is having a wonderful day. He has several of his family members visiting and they went to the lilac festival. Jules Elias called him to express his best wishes and it made his day. Chuck is still working eight hours a week in the special stains lab at University of Rochester and is still involved with the Biological Stains Commission. He did however say that he is phasing out his involvement in presenting and attending histology meetings. We will miss his significant dedication and contribution to our field. Chuck said that the past ten years have been extremely rewarding. Especially with all of the awards he has received. If any of you want to express your wishes, reply to this e-mail and I will forward it on to him. Thank you, Akemi Akemi Allison-Tacha, BS, HT(ASCP)HTL Client Services Manager PhenoPath laboratories 551 North 34th Street, Suite 100 Seattle, WA 98103-8675 Work: (206) 374-9000 ext 1053 E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From JMyers1 <@t> aol.com Sun May 11 19:48:26 2008 From: JMyers1 <@t> aol.com (JMyers1@aol.com) Date: Sun May 11 19:48:40 2008 Subject: [Histonet] IHC Background Staining Message-ID: Karla: It has been my experience that the background you've described is caused by the static that's 'built up' by wiping around the circular hyrdrophobic barrier after rinsing. As other HistoNetters have stated, this can be avoided by applying hyrdrophobic barriers that run the width of the slide, and removing excess buffer by simply tilting the edge of the slide onto an absorbent surface (rather that wiping). Cheers, Joe ------------------------------ Message: 8 Date: Fri, 9 May 2008 16:17:40 -0700 (PDT) From: Karla Arrington Subject: [Histonet] IHC Background Staining To: histonet@lists.utsouthwestern.edu Message-ID: <390591.1467.qm@web32505.mail.mud.yahoo.com> Content-Type: text/plain; charset=us-ascii I do Immuno's by hand and I am getting background DAB precipitate on the inside circle of my sections. What is causing this and how do I get rid of it? **************Wondering what's for Dinner Tonight? Get new twists on family favorites at AOL Food. (http://food.aol.com/dinner-tonight?NCID=aolfod00030000000001) From akemiat3377 <@t> yahoo.com Sun May 11 21:26:11 2008 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Sun May 11 21:26:16 2008 Subject: [Histonet] Chuck Churukian turns 80 today In-Reply-To: <923957.21683.qm@web31308.mail.mud.yahoo.com> Message-ID: <907258.54910.qm@web31304.mail.mud.yahoo.com> Please send your wishes to my personal e-mail at akemiat3377@yahoo.com I am afraid that the histonet might get a little too overloaded. Thanks, Akemi Allison-Tacha, BS, HT(ASCP)HTL Client Services Manager PhenoPath laboratories 551 North 34th Street, Suite 100 Seattle, WA 98103-8675 Work: (206) 374-9000 ext 1053 E-Mail: akemiat3377@yahoo.com --- On Sun, 5/11/08, Akemi Allison-Tacha wrote: > From: Akemi Allison-Tacha > Subject: [Histonet] Chuck Churukian turns 80 today > To: "histo net" > Date: Sunday, May 11, 2008, 4:16 PM > Hi All, > > I just wanted to share with all of you this special day in > history for Chuck Churukian. Please join me in wishing > Chuck a fabulous 80th birthday. I just spoke with him and > he is having a wonderful day. He has several of his family > members visiting and they went to the lilac festival. > Jules Elias called him to express his best wishes and it > made his day. > > Chuck is still working eight hours a week in the special > stains lab at University of Rochester and is still involved > with the Biological Stains Commission. He did however say > that he is phasing out his involvement in presenting and > attending histology meetings. We will miss his significant > dedication and contribution to our field. Chuck said that > the past ten years have been extremely rewarding. > Especially with all of the awards he has received. > > If any of you want to express your wishes, reply to this > e-mail and I will forward it on to him. > > Thank you, > Akemi > > Akemi Allison-Tacha, BS, HT(ASCP)HTL > Client Services Manager > PhenoPath laboratories > 551 North 34th Street, Suite 100 > Seattle, WA 98103-8675 > Work: (206) 374-9000 ext 1053 > E-Mail: akemiat3377@yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Mon May 12 03:14:50 2008 From: louise.renton <@t> gmail.com (louise renton) Date: Mon May 12 03:14:55 2008 Subject: [Histonet] abbreviation of "with" OT Message-ID: I have a great curiosity waiting to be relieved. Ever since I started working in the lab... in those days the pathologist dictated the gross report at cut up and the tech wrote it down...we used the abbreviation of "with" as a "c" with a little horizontal bar above it. Does anyone else use this and if so what is its history? curious Louise -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From HornHV <@t> archildrens.org Mon May 12 07:22:39 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon May 12 07:22:43 2008 Subject: [Histonet] record slides? In-Reply-To: References: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82C38@EMAIL.archildrens.org> Any way you slice it, that's too many slides for one person in one day. Glad I don't work at your lab. My neck, back and shoulder would be screaming at the end of the day. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of CrochiereSteve@aol.com Sent: Friday, May 09, 2008 6:16 PM To: histonet@pathology.swmed.edu Subject: [Histonet] record slides? How many slides have you cut in an 8 hour day? One of my techs today cut 658 prostate bx slides in 7 hours. Just curious. steve **************Wondering what's for Dinner Tonight? Get new twists on family favorites at AOL Food. (http://food.aol.com/dinner-tonight?NCID=aolfod00030000000001) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From b-frederick <@t> northwestern.edu Mon May 12 07:41:20 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon May 12 07:41:32 2008 Subject: [Histonet] record slides? In-Reply-To: Message-ID: <001901c8b42d$7e008370$d00f7ca5@lurie.northwestern.edu> The first thing I think too,is poor person to have no reprieve from cutting. I have constant interruptions, so 658 is not in my book. We cut sterile sections as we cut for DNA/RNA quite a bit and that requires more time in terms of changing blades for every case, cleaning if not changing a waterbath, wiping down the blade holder etc with alcohol, changing gloves and sometimes a mask. We are allowed to do 2 blocks per blade (one on each side).When the study also has 15 slides as well we are lucky to get 15 blocks done in a day.(225 slides +) Routine research we can just whip out, but clinical trials are VERY specific and time consuming. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Saturday, May 10, 2008 1:31 AM To: CrochiereSteve@aol.com Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] record slides? The first thought coming to my mind: poor girl/boy cutting the whole day. I like to cut, but I'am also glad to be ready after max. 3 hours (because of smaller workload, four techs cutting at the same time). Our prostate "menue": 2x HE ? 4 cuts, 1xIHC ? 4 cuts; 10 blocks per patient, without prelabelling; sliding microtome, 20-25 min In the mean we do 60-70 slides (with 1-4 cuts) per hour, without prelabelling, with walking in the staining room, with telephone, .... In my opinion it's a big difference, if one cuts at high speed for a few hours or has to do it the whole shift with the same concentration and quality. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von CrochiereSteve@aol.com Gesendet: Samstag, 10. Mai 2008 01:16 An: histonet@pathology.swmed.edu Betreff: [Histonet] record slides? How many slides have you cut in an 8 hour day? One of my techs today cut 658 prostate bx slides in 7 hours. Just curious. steve **************Wondering what's for Dinner Tonight? Get new twists on family favorites at AOL Food. (http://food.aol.com/dinner-tonight?NCID=aolfod00030000000001) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon May 12 07:55:13 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon May 12 07:55:16 2008 Subject: [Histonet] abbreviation of "with" OT In-Reply-To: Message-ID: <694087.16292.qm@web65710.mail.ac4.yahoo.com> curious Louise: "c" with a little horizontal line above is abbreviation for "cum" = with. Also it is used a small "s" with the same little bar abore as abbreviation of "sine" = without. Ren? J. louise renton wrote: I have a great curiosity waiting to be relieved. Ever since I started working in the lab... in those days the pathologist dictated the gross report at cut up and the tech wrote it down...we used the abbreviation of "with" as a "c" with a little horizontal bar above it. Does anyone else use this and if so what is its history? curious Louise -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From settembr <@t> umdnj.edu Mon May 12 08:07:28 2008 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Mon May 12 08:08:06 2008 Subject: [Histonet] IHC for TFE3 Message-ID: Hello Dr. Cartun, I use TFE-3 from Santa Cruz. It is an RUO the Cat. # is SC-5958. I use it @ 1:1000. It is made in Goat so I use Dako's LSAB+ detection kit. As a pretreatment I use Cell Marque's Trilogy but you can use any EDTA based pretreatment. Thanks for the Mother's Day wishes. Dana Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Richard Cartun 05/11/08 11:08 AM >>> Happy "Mother's Day" to all the moms out there! Does anyone know of a commercially-available antibody to the transcription factor "TFE3" used in the immunohistochemical evaluation of renal tumors? Thank you, Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From froyer <@t> bitstream.net Mon May 12 08:25:12 2008 From: froyer <@t> bitstream.net (Ford Royer) Date: Mon May 12 08:25:22 2008 Subject: [Histonet] What do microtomes retail at? In-Reply-To: References: <007001c8b1c3$7375faf0$0302a8c0@yourxhtr8hvc4p><346E5878979BA54FB4B0BFD6AD93B9B9B01F623355@EXCHMBC1.ad.ah.local> Message-ID: <00ad01c8b433$9bba2aa0$7701a80a@Ford> The last year that it was produced (1997-1999 I think)the list price on a Reichert/Leica 2030 was around $10,000.00. Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL:? 612-839-1046 Phone:? 763-542-8725 Fax:? 763-546-4830 eMail:? clinicallab@minnesotamedical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Merced Leiker Sent: Friday, May 09, 2008 12:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] What do microtomes retail at? Anyone know what a basic microtome retails at? I'm looking at purchasing a refurbished Leica/Reichert 2030 for $3000 and just wanted to know what a similar one costs new. Thanks! Merced M Leiker Research Technician II 354 BRB (Lee Lab) / 140 Farber Hall (mail) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Mon May 12 09:11:38 2008 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Mon May 12 09:11:43 2008 Subject: [Histonet] abbreviation of "with" OT Message-ID: <5C0BED61F529364E86309CADEA63FEF20163F3F3@TRFT-EX01.xRothGen.nhs.uk> Congratulations Rene. You are the first to get that dirty word through our new e-mail filtering system. I suppose it is the inverted commas that did it. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 12 May 2008 13:55 To: louise renton; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] abbreviation of "with" OT curious Louise: "c" with a little horizontal line above is abbreviation for "cum" = with. Also it is used a small "s" with the same little bar abore as abbreviation of "sine" = without. Ren? J. louise renton wrote: I have a great curiosity waiting to be relieved. Ever since I started working in the lab... in those days the pathologist dictated the gross report at cut up and the tech wrote it down...we used the abbreviation of "with" as a "c" with a little horizontal bar above it. Does anyone else use this and if so what is its history? curious Louise -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vazquezr <@t> ohsu.edu Mon May 12 09:25:13 2008 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Mon May 12 09:25:43 2008 Subject: [Histonet] abbreviation of "with" OT Message-ID: Louise, I even use it when I make out my shopping list at home and writing info down when talking with someone on the phone. It is medical shorthand, I don't know history, you could wikipedia it (everything is in that digest). Rene, potty mouth, potty mouth...haha Robyn >>> "Marshall Terry Dr, Consultant Histopathologist" 5/12/2008 7:11 AM >>> Congratulations Rene. You are the first to get that dirty word through our new e-mail filtering system. I suppose it is the inverted commas that did it. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 12 May 2008 13:55 To: louise renton; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] abbreviation of "with" OT curious Louise: "c" with a little horizontal line above is abbreviation for "cum" = with. Also it is used a small "s" with the same little bar abore as abbreviation of "sine" = without. Ren? J. louise renton wrote: I have a great curiosity waiting to be relieved. Ever since I started working in the lab... in those days the pathologist dictated the gross report at cut up and the tech wrote it down...we used the abbreviation of "with" as a "c" with a little horizontal bar above it. Does anyone else use this and if so what is its history? curious Louise -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Mon May 12 09:33:29 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Mon May 12 09:33:37 2008 Subject: [Histonet] abbreviation of "with" OT In-Reply-To: Message-ID: I have always used that "c" designation but I don't know where I picked it up. In MS word it is found in "insert" under "symbols". Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: Monday, May 12, 2008 3:15 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] abbreviation of "with" OT I have a great curiosity waiting to be relieved. Ever since I started working in the lab... in those days the pathologist dictated the gross report at cut up and the tech wrote it down...we used the abbreviation of "with" as a "c" with a little horizontal bar above it. Does anyone else use this and if so what is its history? curious Louise -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ploykasek <@t> phenopath.com Mon May 12 09:48:14 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Mon May 12 09:48:35 2008 Subject: [Histonet] IHC for TFE3 In-Reply-To: <4826D3A9020000770000C805@gwmail4.harthosp.org> Message-ID: Hi Rich. Were you working on Mother's Day?? I hope you are not working too hard. We are using the Santa Cruz goat TFE3 antibody their clone p16. We use a heat citrate retrieval. We have been using the LSAB+ Dako detection kit, but are trying the anti-goat polymer kit from Biocare. If you need any further info, just let me know. Have a good week. We're hoping for a bit of sunshine here in the Northwest. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Happy "Mother's Day" to all the moms out there! > > Does anyone know of a commercially-available antibody to the transcription > factor "TFE3" used in the immunohistochemical evaluation of renal tumors? > > Thank you, > > Richard > > Richard W. Cartun, Ph.D. > Director, Immunopathology & Histology > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > > Confidentiality Notice > > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential or proprietary information > which is legally privileged. Any unauthorized review, use, disclosure, or > distribution is prohibited. If you are not the intended recipient, please > promptly contact the sender by reply e-mail and destroy all copies of the > original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From Jackie.O'Connor <@t> abbott.com Mon May 12 09:48:32 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Mon May 12 09:48:55 2008 Subject: [Histonet] 4% paraformaldehyde all over again In-Reply-To: <5F31F38C96781A4FBE3196EBC22D47807F2621@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: Will vendors who contacted me off line regarding supplying vast quantities of 4% paraformaldehyde contact me again, please. Thanks. Jackie O'Connor Abbott Labs From Jackie.O'Connor <@t> abbott.com Mon May 12 09:49:54 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Mon May 12 09:50:11 2008 Subject: [Histonet] abbreviation of "with" OT In-Reply-To: Message-ID: Latin. C = cum (with) as in cum laude. S =sans (without) as in sans underwear. "Sebree Linda A." Sent by: histonet-bounces@lists.utsouthwestern.edu 05/12/2008 09:33 AM To "louise renton" , cc Subject RE: [Histonet] abbreviation of "with" OT I have always used that "c" designation but I don't know where I picked it up. In MS word it is found in "insert" under "symbols". Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: Monday, May 12, 2008 3:15 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] abbreviation of "with" OT I have a great curiosity waiting to be relieved. Ever since I started working in the lab... in those days the pathologist dictated the gross report at cut up and the tech wrote it down...we used the abbreviation of "with" as a "c" with a little horizontal bar above it. Does anyone else use this and if so what is its history? curious Louise -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Mon May 12 10:02:12 2008 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Mon May 12 10:02:16 2008 Subject: Fw: [Histonet] Chuck Churukian turns 80 today Message-ID: <429565.37130.qm@web31302.mail.mud.yahoo.com> Hi All, I just wanted to share with all of you this special day in history for Chuck Churukian. Please join me in wishing Chuck a fabulous 80th birthday. I just spoke with him and he is having a wonderful day. He has several of his family members visiting and they went to the lilac festival. Jules Elias called him to express his best wishes and it made his day. Chuck is still working eight hours a week in the special stains lab at University of Rochester and is still involved with the Biological Stains Commission. He did however say that he is phasing out his involvement in presenting and attending histology meetings. We will miss his significant contribution and dedication to our field. Chuck said that the past ten years have been extremely rewarding. Especially with all of the awards he has received. If any of you want to express your wishes, reply to this e-mail and I will forward it on to him. Thank you, Akemi Akemi Allison-Tacha, BS, HT(ASCP)HTL Client Services Manager PhenoPath laboratories 551 North 34th Street, Suite 100 Seattle, WA 98103-8675 Work: (206) 374-9000 ext 1053 E-Mail: akemiat3377@yahoo.com> > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Mon May 12 10:39:31 2008 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Mon May 12 10:40:47 2008 Subject: [Histonet] abbreviation of "with" OT References: Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2642@fhosxchmb006.ADVENTISTCORP.NET> We could use w/ to mean with...you know, so as not to offend...... ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Robyn Vazquez Sent: Mon 5/12/2008 10:25 AM To: louise.renton@gmail.com; Histonet@lists.utsouthwestern.edu; Terry.Marshall@rothgen.nhs.uk; rjbuesa@yahoo.com Subject: RE: [Histonet] abbreviation of "with" OT Louise, I even use it when I make out my shopping list at home and writing info down when talking with someone on the phone. It is medical shorthand, I don't know history, you could wikipedia it (everything is in that digest). Rene, potty mouth, potty mouth...haha Robyn >>> "Marshall Terry Dr, Consultant Histopathologist" 5/12/2008 7:11 AM >>> Congratulations Rene. You are the first to get that dirty word through our new e-mail filtering system. I suppose it is the inverted commas that did it. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 12 May 2008 13:55 To: louise renton; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] abbreviation of "with" OT curious Louise: "c" with a little horizontal line above is abbreviation for "cum" = with. Also it is used a small "s" with the same little bar abore as abbreviation of "sine" = without. Ren? J. louise renton wrote: I have a great curiosity waiting to be relieved. Ever since I started working in the lab... in those days the pathologist dictated the gross report at cut up and the tech wrote it down...we used the abbreviation of "with" as a "c" with a little horizontal bar above it. Does anyone else use this and if so what is its history? curious Louise -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ============================================================================== The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ============================================================================== From kalschev <@t> svm.vetmed.wisc.edu Mon May 12 10:47:37 2008 From: kalschev <@t> svm.vetmed.wisc.edu (Vicki Kalscheur) Date: Mon May 12 10:48:21 2008 Subject: [Histonet] Fw: Job opening Message-ID: <003701c8b447$80b6f210$c5d76880@vetmed.wisc.edu> Members and Friends: VK The job description is as follows: New York University College of Dentistry (NYUCD) is searching to fill a full-time technical position in our hard tissue preparation laboratory. The person should have experience in the cleaning, embedding (e.g. PMMA), sectioning, grinding and polishing mineralized bones and teeth. Our preparation laboratory contains a number of Buehler instruments for this work, but a new Exakt thin sectioning and grinding system has just been installed, and experience with this technology would also be a significant advantage. The person will also participate in the light and scanning electron imaging of prepared specimens. Please contact Tim Bromage, NYUCD, at tim.bromage@nyu.edu for more information. From Heather.D.Renko <@t> osfhealthcare.org Mon May 12 10:54:05 2008 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Mon May 12 10:54:15 2008 Subject: [Histonet] re: microtomes Message-ID: <40026EDDE64CDA47AB382C52619ACD3C0A09DF1F@pmc-rfd-mx01.intranet.osfnet.org> I purchased a basic manual Leica 2125 about two years ago for under $5000.00 and it's a gem of an instrument. Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From rjbuesa <@t> yahoo.com Mon May 12 11:04:31 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon May 12 11:04:37 2008 Subject: [Histonet] abbreviation of "with" OT In-Reply-To: Message-ID: <976044.36006.qm@web65711.mail.ac4.yahoo.com> "Sans" is the French derivative from the Latin "sine" (without). Ren? J. Jackie M O'Connor wrote: Latin. C = cum (with) as in cum laude. S =sans (without) as in sans underwear. "Sebree Linda A." Sent by: histonet-bounces@lists.utsouthwestern.edu 05/12/2008 09:33 AM To "louise renton" , cc Subject RE: [Histonet] abbreviation of "with" OT I have always used that "c" designation but I don't know where I picked it up. In MS word it is found in "insert" under "symbols". Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: Monday, May 12, 2008 3:15 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] abbreviation of "with" OT I have a great curiosity waiting to be relieved. Ever since I started working in the lab... in those days the pathologist dictated the gross report at cut up and the tech wrote it down...we used the abbreviation of "with" as a "c" with a little horizontal bar above it. Does anyone else use this and if so what is its history? curious Louise -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From MadaryJ <@t> MedImmune.com Mon May 12 11:11:47 2008 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Mon May 12 11:12:19 2008 Subject: [Histonet] Slides in a day Message-ID: Stevo-Nick here did the tech cut that many slides or blocks? Guessing slides since prostates are usually levels etc. How many blocks were cut? I mean, that is the question. Were the blocks rough cut already, were the slides made already? Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Laboratory One Medimmune Way Gaithersburg, MD 20878 ph 301.398.4745/6360 fx 301.398.9745 To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From Jackie.O'Connor <@t> abbott.com Mon May 12 11:17:24 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Mon May 12 11:17:41 2008 Subject: [Histonet] abbreviation of "with" OT In-Reply-To: <976044.36006.qm@web65711.mail.ac4.yahoo.com> Message-ID: Thanks for the clarification - I took Latin for 4 years, in the late 60's. I guess I forgot a couple of words. Still, sine underwear doesn't have the same impact as sans underwear. Rene J Buesa 05/12/2008 11:04 AM To Jackie M O'Connor , "Sebree Linda A." cc Histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Subject RE: [Histonet] abbreviation of "with" OT "Sans" is the French derivative from the Latin "sine" (without). Ren? J. Jackie M O'Connor wrote: Latin. C = cum (with) as in cum laude. S =sans (without) as in sans underwear. "Sebree Linda A." Sent by: histonet-bounces@lists.utsouthwestern.edu 05/12/2008 09:33 AM To "louise renton" , cc Subject RE: [Histonet] abbreviation of "with" OT I have always used that "c" designation but I don't know where I picked it up. In MS word it is found in "insert" under "symbols". Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: Monday, May 12, 2008 3:15 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] abbreviation of "with" OT I have a great curiosity waiting to be relieved. Ever since I started working in the lab... in those days the pathologist dictated the gross report at cut up and the tech wrote it down...we used the abbreviation of "with" as a "c" with a little horizontal bar above it. Does anyone else use this and if so what is its history? curious Louise -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From sbreeden <@t> nmda.nmsu.edu Mon May 12 11:20:55 2008 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Mon May 12 11:21:03 2008 Subject: [Histonet] Latin - Thank goodness! Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E62FA@nmdamailsvr.nmda.ad.nmsu.edu> When I was beginning high school (gulp - 1961), my mother INSISTED that I take Latin. I could not imagine what Latin would do for me in the long run, but I have thanked mother many, many times for insisting. Not only is it priceless in the medical field, but just doing crossword puzzles is made easier by knowing Latin. I can still recite "Gaul is divided into three parts..."! I've used the c-with-the-little-bar and the s-with-the-little-bar in practically everything (grocery lists, college class notes, doing gross cut-in, etc.) and have many times been asked what that means! And, it's pronounced "coom" which relieves the pronunciation anxiety and raised eyebrows. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From Jackie.O'Connor <@t> abbott.com Mon May 12 11:22:05 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Mon May 12 11:22:22 2008 Subject: [Histonet] Slides in a day In-Reply-To: Message-ID: Our techs can comfortably cut 120 blocks of animal tissue per 8 hour shift, which includes facing. Any more than that is pushing it, so 120 is our expectation. I have personally cut 300 slides in one day, but that included multiple sections from each block. "Madary, Joseph" Sent by: histonet-bounces@lists.utsouthwestern.edu 05/12/2008 11:11 AM To cc Subject [Histonet] Slides in a day Stevo-Nick here did the tech cut that many slides or blocks? Guessing slides since prostates are usually levels etc. How many blocks were cut? I mean, that is the question. Were the blocks rough cut already, were the slides made already? Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Laboratory One Medimmune Way Gaithersburg, MD 20878 ph 301.398.4745/6360 fx 301.398.9745 To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asmith <@t> mail.barry.edu Mon May 12 11:51:53 2008 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Mon May 12 11:52:06 2008 Subject: [Histonet] RE: Latin - Thank goodness! In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E62FA@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B017E62FA@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: Latin is also useful in law. (A sponge left in the pelvic cavity is a case of "Res ipsa loquitur.") Latin also helps in puzzling out articles in Spanish or Portuguese. Most of all Latin gives access to a great literature that has been rather poorly translated. No translation of the Aeneid give one any conception of the magnificence of the original. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Monday, May 12, 2008 12:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Latin - Thank goodness! When I was beginning high school (gulp - 1961), my mother INSISTED that I take Latin. I could not imagine what Latin would do for me in the long run, but I have thanked mother many, many times for insisting. Not only is it priceless in the medical field, but just doing crossword puzzles is made easier by knowing Latin. I can still recite "Gaul is divided into three parts..."! I've used the c-with-the-little-bar and the s-with-the-little-bar in practically everything (grocery lists, college class notes, doing gross cut-in, etc.) and have many times been asked what that means! And, it's pronounced "coom" which relieves the pronunciation anxiety and raised eyebrows. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MadaryJ <@t> MedImmune.com Mon May 12 12:08:59 2008 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Mon May 12 12:09:47 2008 Subject: [Histonet] C =Con(with) S means Sans(without)-Latin origin Message-ID: Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Laboratory One Medimmune Way Gaithersburg, MD 20878 ph 301.398.4745/6360 fx 301.398.9745 To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From Janet.Bonner <@t> FLHOSP.ORG Mon May 12 12:19:14 2008 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Mon May 12 12:20:22 2008 Subject: [Histonet] C =Con(with) S means Sans(without)-Latin origin References: Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2647@fhosxchmb006.ADVENTISTCORP.NET> OK, I use w/ = with, and w/o = without. -American ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Madary, Joseph Sent: Mon 5/12/2008 1:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] C =Con(with) S means Sans(without)-Latin origin Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Laboratory One Medimmune Way Gaithersburg, MD 20878 ph 301.398.4745/6360 fx 301.398.9745 To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ============================================================================== The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ============================================================================== From freckles9660 <@t> yahoo.com Mon May 12 14:56:58 2008 From: freckles9660 <@t> yahoo.com (Karla Arrington) Date: Mon May 12 14:57:00 2008 Subject: [Histonet] ICC problem Message-ID: <251789.53346.qm@web32506.mail.mud.yahoo.com> Hello all.. I currently perform IHC's by hand.? Although they are beautiful, I do get a faint background staining inside the circle of the hydropholic pen.? It looks like excess DAB stain. I do not wipe around the tissues causing no static.? Any suggestions on how to fix this? Karla Arrington ____________________________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ From freckles9660 <@t> yahoo.com Mon May 12 15:04:05 2008 From: freckles9660 <@t> yahoo.com (Karla Arrington) Date: Mon May 12 15:04:08 2008 Subject: [Histonet] Bone Marrow Staining Message-ID: <92592.51349.qm@web32502.mail.mud.yahoo.com> I currently use 10% NBF for fixing bone marrows (core and clot). Although lately the sections appear "washed out". I previously used B-5 Fixative. Is there a better fixative for fixing bone marrows so their cells are crisp and clear? Karla Arrington freckles9660@yahoo.com ____________________________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ From rjbuesa <@t> yahoo.com Mon May 12 15:17:13 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon May 12 15:17:16 2008 Subject: [Histonet] Bone Marrow Staining In-Reply-To: <92592.51349.qm@web32502.mail.mud.yahoo.com> Message-ID: <604837.79006.qm@web65710.mail.ac4.yahoo.com> The "washed out" appearance most likely has nothing to do with fixation. Check if you are heating the sections BEFORE they are completely drained off. That can cause that artifact. The only thing you have to be aware of when using NBF to fix BM specimens is to control the pH on the staining solutions (specially the Giemsa). Ren? J. Karla Arrington wrote: I currently use 10% NBF for fixing bone marrows (core and clot). Although lately the sections appear "washed out". I previously used B-5 Fixative. Is there a better fixative for fixing bone marrows so their cells are crisp and clear? Karla Arrington freckles9660@yahoo.com ____________________________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From bakevictoria <@t> gmail.com Mon May 12 15:33:38 2008 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Mon May 12 15:33:44 2008 Subject: [Histonet] IHC Background Staining In-Reply-To: <390591.1467.qm@web32505.mail.mud.yahoo.com> References: <390591.1467.qm@web32505.mail.mud.yahoo.com> Message-ID: <4f016b690805121333m20c00719v77da5f74d5645c28@mail.gmail.com> What everyone else is saying is true and you will need to check your procedure carefully. Manual staining is a technique that is difficult with some antibodies and especially when working with a large slide number. One of the things that I did was eliminate using a pap pen. I used 2 X 2 strips of parafilm that I cut in strips of 1 X 2 and layed them on the tissue/slide after the reagent has been applied. To remove I dipped the slides in buffer and the film would slide right off. Good luck. I did IHC manually for a long time and it is not always easy. Vikki On 5/9/08, Karla Arrington wrote: > I do Immuno's by hand and I am getting background DAB precipitate on the inside circle of my sections. > What is causing this and how do I get rid of it? > > > ____________________________________________________________________________________ > Be a better friend, newshound, and > know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tshrobertson <@t> yahoo.com Mon May 12 16:27:43 2008 From: tshrobertson <@t> yahoo.com (Teisha Robertson) Date: Mon May 12 16:27:47 2008 Subject: [Histonet] non specific staining Message-ID: <605614.77385.qm@web62515.mail.re1.yahoo.com> how do you eliminate non specific staining in olfactory bulb sections? --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From CIngles <@t> uwhealth.org Mon May 12 16:39:43 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Mon May 12 16:42:07 2008 Subject: [Histonet] RE: Latin - Thank goodness! References: <4D14F0FC9316DD41972D5F03C070908B017E62FA@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <08A0A863637F1349BBFD83A96B27A50A120128@uwhis-xchng3.uwhis.hosp.wisc.edu> I'm a youngster, but I also bought a latin dictionary just for the medical terminology aspect. Good thing I didn't have to take it. It is interesting stuff, but I'm terrible at learning languages. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Smith, Allen Sent: Mon 5/12/2008 11:51 AM To: 'Breeden, Sara' Cc: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Latin - Thank goodness! Latin is also useful in law. (A sponge left in the pelvic cavity is a case of "Res ipsa loquitur.") Latin also helps in puzzling out articles in Spanish or Portuguese. Most of all Latin gives access to a great literature that has been rather poorly translated. No translation of the Aeneid give one any conception of the magnificence of the original. From tjasper <@t> copc.net Mon May 12 17:52:06 2008 From: tjasper <@t> copc.net (Thomas Jasper) Date: Mon May 12 17:52:13 2008 Subject: [Histonet] I need more ammunition References: Message-ID: <90354A475B420441B2A0396E5008D4965E20AF@copc-sbs.COPC.local> Hi Wayne, I know I already sent you a reply on this topic (off-line). Just had one more thought...you might want to point out to the "powers that be" that your organization is running a HUGE risk for the ULTIMATE in legal nightmares. Nothing gets the attention of "higher ups" quicker than $$$$$$'s and lawsuits. In the end Wayne, if you do not get anywhere with this group, I would seriously consider seeking employment elsewhere. That's not always the most desirable move, but let's face it...you are currently in a position and a market, which makes you a hot commodity. Secondly, you don't need to be implicated in any legal entanglements due to poor decisions (or lack of them) by the folks in authority where you work. Watch out for yourself man it's tough to speak truth to power. I pity the poor patients as well. Good luck, Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WAYNE HOLLAND Sent: Saturday, May 10, 2008 5:31 PM To: Histonet@lists.utsouthwestern.edu Subject: FW: RE: [Histonet] I need more ammunition I need more ammunition, please!! >From: WAYNE HOLLAND >Sent: Fri 5/9/2008 1:47 AM >To: Histonet@lists.utsouthwestern.edu >Subject: [Histonet] I need your help > > >Everyone, I have started a new job. I have many things that need fixed. >I have a gross room that are using regular cassettes metal tops and >they are wrapping all of the derm work in yes wet lens paper and >leaving them on the countertop by the hoods for periods of up to 45 >minutes. I know this is not good for obvious reasons. I need your >comments asap to help make them understand, again for obvious reasons. >I have been doing this for 28 years and I need other field associates >to back me up. Most of these specimen are very small. HELP! > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >NOTICE OF CONFIDENTIALITY >This electronic message, including attachments, is for the sole use of >the named recipient and may contain confidential or privileged >information protected by New York State, and Federal regulations. Any >unauthorized review, use, disclosure, copying or distribution is >strictly prohibited. If you are not the intended recipient or have >received this communication in error please contact the sender or >email.security@bassett.org and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Carrie.Oto <@t> clinlab.ucsfmedctr.org Mon May 12 18:26:06 2008 From: Carrie.Oto <@t> clinlab.ucsfmedctr.org (Oto, Carrie) Date: Mon May 12 18:25:47 2008 Subject: [Histonet] Method validation Message-ID: <00EC53C1C120524B80A74AC951675233187E2C@CB-LIS-APSVR-1.ucsfmedicalcenter.org> Our Histology lab will be moving from one campus to another, 2 seperate CLIA's. Accrediting agency is Joint Commission. What, if any, has other labs done for method validation of histology equipment and reagents? For example, processor, stainer, IPOX stains, etc. Any and all information is appreciated especially if there is specific information for California labs. From jnocito <@t> satx.rr.com Mon May 12 20:56:18 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon May 12 20:56:08 2008 Subject: [Histonet] C =Con(with) S means Sans(without)-Latin origin References: <5F31F38C96781A4FBE3196EBC22D47807F2647@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: <006b01c8b49c$898769b0$0302a8c0@yourxhtr8hvc4p> yeah, you go girl JTT ----- Original Message ----- From: "Bonner, Janet" To: "Madary, Joseph" ; Sent: Monday, May 12, 2008 12:19 PM Subject: RE: [Histonet] C =Con(with) S means Sans(without)-Latin origin OK, I use w/ = with, and w/o = without. -American ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Madary, Joseph Sent: Mon 5/12/2008 1:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] C =Con(with) S means Sans(without)-Latin origin Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Laboratory One Medimmune Way Gaithersburg, MD 20878 ph 301.398.4745/6360 fx 301.398.9745 To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ============================================================================== The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Tue May 13 01:37:16 2008 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue May 13 01:37:19 2008 Subject: [Histonet] 4% paraformaldehyde all over again In-Reply-To: References: <5F31F38C96781A4FBE3196EBC22D47807F2621@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: There is no such thing as 4% paraformaldehyde. Nobody can sell or us nauseam. said, in 3 words, vomit". Latin is concise formaldehyde is a real solution, and solution is to heat 40 grammes of  litre of water, with an alkaline catalyst to sp hydrolysis. Paraformaldehyde exists only as a solid substan which is insoluble in water.  All this has been in all the textbooks and manuals for 40+ years.
 
Joh UWO
London, Canada
Original Message -----
From: <Jackie.O'Connor@abbott.com>< Monday, May 12, 2008 10:52
Subject: [Histo 4% paraformaldehyde all over again
To: Histonet < Histonet@lists.utsouthwestern.edu>, histonet-bounces@li sts.utsouthwestern.edu

> Will vendors who cont quantities < again, please. <
> Jackie O'Conn Labs
> _________ _______________________ 5F Histonet mail Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Tue May 13 04:02:22 2008 From: louise.renton <@t> gmail.com (louise renton) Date: Tue May 13 04:02:26 2008 Subject: [Histonet] thanks Message-ID: thanks to all who took the time to respond to my OT question. Now i can sleep nights.......... -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From asmith <@t> mail.barry.edu Tue May 13 08:07:41 2008 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Tue May 13 08:07:57 2008 Subject: [Histonet] 4% paraformaldehyde all over again In-Reply-To: References: <5F31F38C96781A4FBE3196EBC22D47807F2621@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: It appears that Dr. Kiernan is another victim of Microsoft office 2007. Could a users' coalition large enough to boycott it effectively be formed? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Kiernan Sent: Tuesday, May 13, 2008 2:37 AM To: Jackie M O'Connor Cc: Histonet; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] 4% paraformaldehyde all over again There is no such thing as 4% paraformaldehyde. Nobody can sell or us=uch a non-substance!
See Histonet archives passim ad nauseam.=6nbsp; (That's how the ancient Romans said, in 3 words,=2on and on, until you want to vomit". Latin is concise=
 
4% formaldehyde is a real solution, and =e of the ways of making the solution is to heat 40 grammes of =raformaldehyde in a litre of water, with an alkaline catalyst to sp?d up the hydrolysis. Paraformaldehyde exists only as a solid substan?, which is insoluble in water.  All this has been in all the textbooks and manuals for 40+ years.
 
Joh=iernan
Anatomy,  UWO
London, Canada
=D = = = =
----- Original Message -----
From: =ckie M O'Connor <Jackie.O'Connor@abbott.com><=>Date: Monday, May 12, 2008 10:52
Subject: [Histo=t] 4% paraformaldehyde all over again
To: Histonet < Histonet@lists.utsouthwestern.edu>, histonet-bounces@li sts.utsouthwestern.edu

> Will vendors who cont?ted me off line regarding supplying vast
> quantities <=>> of 4% paraformaldehyde contact me again, please. <=>> Thanks.
>
> Jackie O'Conn=
> Abbott Labs
> _________ _______________________ 5F=F_____________
> Histonet mail=g list
> Histonet@lists.utsouthwestern.edu
=6gt; http://lists.utsouthwestern.edu/mailman/listinfo/histonet From portera <@t> msu.edu Tue May 13 09:49:40 2008 From: portera <@t> msu.edu (Amy Porter) Date: Tue May 13 09:49:44 2008 Subject: [Histonet] Catalase Staining for Liver Aspirates Help Please Message-ID: <001201c8b508$92a98c50$8e7a0923@histolab> Hello to all - I am looking for an enzyme staining method to demonstrate peroxisomes (catalase specifically) in Canine Liver aspirate smears. If anyone out there has anything they would be will to share it would be so appreciated. I have spent a great deal of time searching on the web and have not yet found a methodology. Thanks in advance for any assistance. Amy Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu From akbitting <@t> geisinger.edu Tue May 13 10:23:17 2008 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Tue May 13 10:23:45 2008 Subject: [Histonet] Amended reports- Cerner CoPath users Message-ID: <48297A25.2B7F.00C9.0@geisinger.edu> I've got a question for users of Cerner Copath. Do you have CoPath set up so that when a report is amended the original diagnosis remains visible along with the amended diagnosis? Also, is there some type of flag at the top of the report to alert a physician that there have been changes to the report? Thanks for your help on this one. Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From nancy_schmitt <@t> pa-ucl.com Tue May 13 11:28:24 2008 From: nancy_schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Tue May 13 11:28:37 2008 Subject: [Histonet] Standardized Microtomes Message-ID: <9FC023A4AB52BB4D87DC6456081A822C087C8D@mercury.pa-ucl.com> Good Morning to all Does anybody standardize microtomes so they all cut at same angle? Is this impossible? We only cut control blocks on one microtome because they are each at a little different angle. Would be nice to be able to do at all........... Thanks for any feedback Nancy Schmitt Histology Coordinator Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From mwich <@t> 7thwavelabs.com Tue May 13 11:30:13 2008 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Tue May 13 11:30:23 2008 Subject: [Histonet] Apipophilin and MCM Message-ID: <62A8156F8071C8439080D626DF8C33A602E3F5@wave-mail.7thwave.local> Does anyone know if Apipophilin and MCM (mini chromosome maintenance protein) are commercially available and, if so, who sells them? Thanks for any info! This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From gentras <@t> vetmed.auburn.edu Tue May 13 11:35:58 2008 From: gentras <@t> vetmed.auburn.edu (Atoska Gentry) Date: Tue May 13 11:36:07 2008 Subject: [Histonet] rubber stoppers & tubing Message-ID: <4829C36E.8000504@vetmed.auburn.edu> hello, if any of you have a source for two-hole black rubber stoppers measuring : 21mm top x 13mm bottom x 7mm in length ( with the inscription 304w SET on top, and with one hole measuring 2-3mm and the other 3-4 mm); and or 15mm bottom x 8mm in length; also, rigid plastic tubing 2-3 mm in diameter, 196mm in length will you please contact me ASAP? Thank you kindly, Atoska -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu From liz <@t> premierlab.com Tue May 13 11:38:18 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue May 13 11:38:27 2008 Subject: [Histonet] Standardized Microtomes In-Reply-To: <9FC023A4AB52BB4D87DC6456081A822C087C8D@mercury.pa-ucl.com> References: <9FC023A4AB52BB4D87DC6456081A822C087C8D@mercury.pa-ucl.com> Message-ID: Newcomer supply has a device that will standardize microtomes. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Tuesday, May 13, 2008 10:28 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Standardized Microtomes Good Morning to all Does anybody standardize microtomes so they all cut at same angle? Is this impossible? We only cut control blocks on one microtome because they are each at a little different angle. Would be nice to be able to do at all........... Thanks for any feedback Nancy Schmitt Histology Coordinator Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Tue May 13 11:41:10 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue May 13 11:41:26 2008 Subject: [Histonet] Standardized Microtomes In-Reply-To: <9FC023A4AB52BB4D87DC6456081A822C087C8D@mercury.pa-ucl.com> Message-ID: Get a block aligner. We have aligned all of our microtomes so that any technicican can do a recut no matter what machine it was originally cut on. Nancy Schmitt Sent by: histonet-bounces@lists.utsouthwestern.edu 05/13/2008 11:28 AM To "'histonet@lists.utsouthwestern.edu'" cc Subject [Histonet] Standardized Microtomes Good Morning to all Does anybody standardize microtomes so they all cut at same angle? Is this impossible? We only cut control blocks on one microtome because they are each at a little different angle. Would be nice to be able to do at all........... Thanks for any feedback Nancy Schmitt Histology Coordinator Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From arizzo <@t> mcw.edu Tue May 13 11:54:13 2008 From: arizzo <@t> mcw.edu (amy rizzo) Date: Tue May 13 11:54:20 2008 Subject: [Histonet] Paraffin thickness standard or measurement? Message-ID: <000001c8b519$f8d86440$c91e3086@marqnet.mu.edu> Is there anyway to measure the thickness of your paraffin section as it comes off a microtome? I have noticed mine isn't as consistent as I would like. Do they sell something anywhere that does this? Thanks, Amy Rizzo From jcline <@t> wchsys.org Tue May 13 11:56:55 2008 From: jcline <@t> wchsys.org (Joyce Cline) Date: Tue May 13 11:57:06 2008 Subject: [Histonet] Standardized Microtomes In-Reply-To: <9FC023A4AB52BB4D87DC6456081A822C087C8D@mercury.pa-ucl.com> Message-ID: <001201c8b51a$5996a080$1d2a14ac@wchsys.org> Each week we align all our microtomes. A company called Advance Innovations has created an aligner that is available through several companies. Aligning our microtomes prevents losing tissue from small biopsies when a recut is called for. ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Good Morning to all Does anybody standardize microtomes so they all cut at same angle? Is this impossible? We only cut control blocks on one microtome because they are each at a little different angle. Would be nice to be able to do at all........... Thanks for any feedback Nancy Schmitt Histology Coordinator Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From Jackie.O'Connor <@t> abbott.com Tue May 13 12:05:29 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue May 13 12:05:47 2008 Subject: [Histonet] Paraffin thickness standard or measurement? In-Reply-To: <000001c8b519$f8d86440$c91e3086@marqnet.mu.edu> Message-ID: I don't think you can buy them legally - they are called 'eyes'. (ha!) You should be able to tell on the stained slide if you sections are not consistent. Usually, if you are getting thick and thin ribbons, something is loose on your microtome. clean off all paraffin debris - sometimes knives can torque from paraffin in the knife holder - paraffin generally gets in the way of tightening anything appropriately. Good luck. Jackie O' amy rizzo Sent by: histonet-bounces@lists.utsouthwestern.edu 05/13/2008 11:54 AM To cc Subject [Histonet] Paraffin thickness standard or measurement? Is there anyway to measure the thickness of your paraffin section as it comes off a microtome? I have noticed mine isn't as consistent as I would like. Do they sell something anywhere that does this? Thanks, Amy Rizzo _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgalnow <@t> aol.com Tue May 13 12:23:37 2008 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Tue May 13 12:23:46 2008 Subject: [Histonet] salary scales Message-ID: <8CA833536AA84EE-B60-1EFF@FWM-M44.sysops.aol.com> I know that this is a sore subject, but typically at what part of the range do you brings histotechnologists in at?? And if you wouldn't mind sharing the range I would be grateful.? I work for a company that is run by business people not lab people and I am trying to convince them to adjust the salaries.? For instance, our histotechnician range is 17.96 - 21.45, our histotechnologist range is 21.14 - 26.58, and our lab supervisor range is 25.46 - 34.28. Thanks in advance, Roxanne From TJJ <@t> Stowers-Institute.org Tue May 13 12:46:40 2008 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Tue May 13 12:47:00 2008 Subject: [Histonet] Sakura VIP flood? Message-ID: Any of you users of the Sakura VIP 5 have any problems with the fume control water somehow siphoning and flooding into the guts of the instrument? The manual tells us to put between 2-3 liters of water in there. We had issues with this happening early on, and were told we had too much water in there. We now use much less, and until 3 days ago had no problem with it. This morning came in to water all in the overflow tray, dripping down from the inside of the instrument on to the floor, and an empty fume control water bottle. My in house service guy will be calling Sakura today, but wanted to know of others' experiences with this issue. I'm not interested in bashing the product, it's worked well for us for over 6 years. Just wondering if it's happened to anybody else. Thanks! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From mpence <@t> grhs.net Tue May 13 12:59:04 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Tue May 13 12:59:09 2008 Subject: [Histonet] Help! Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A382E@IS-E2K3.grhs.net> I am in need of a calretinin control block. Can anyone tell me of anything other than lung mesothelioma tissue that will work in a pinch or if anyone would have a block you would be willing to send me? I will cover the shipping. Thanks, Mike Pence Great River Medical Center Dept. of Pathology West Burlington, IA From jqb7 <@t> cdc.gov Tue May 13 13:13:20 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Tue May 13 13:21:14 2008 Subject: [Histonet] Sakura VIP flood? In-Reply-To: References: Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A747A349@LTA3VS011.ees.hhs.gov> We fill to about 2.5 and have had no problems. Yet. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Teri Sent: Tuesday, May 13, 2008 1:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura VIP flood? Any of you users of the Sakura VIP 5 have any problems with the fume control water somehow siphoning and flooding into the guts of the instrument? The manual tells us to put between 2-3 liters of water in there. We had issues with this happening early on, and were told we had too much water in there. We now use much less, and until 3 days ago had no problem with it. This morning came in to water all in the overflow tray, dripping down from the inside of the instrument on to the floor, and an empty fume control water bottle. My in house service guy will be calling Sakura today, but wanted to know of others' experiences with this issue. I'm not interested in bashing the product, it's worked well for us for over 6 years. Just wondering if it's happened to anybody else. Thanks! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HDOWNS <@t> PARTNERS.ORG Tue May 13 13:26:28 2008 From: HDOWNS <@t> PARTNERS.ORG (Downs, Heather M.) Date: Tue May 13 13:26:36 2008 Subject: [Histonet] 4% paraformaldehyde all over again In-Reply-To: References: Message-ID: We purchase paraformaldehyde in powder form, from Fisher, and make our own 4% paraformaldehyde. We use it for perfusion, of animals and making PLP for our biopsies. Heather -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, May 13, 2008 1:03 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 54, Issue 17 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. C =Con(with) S means Sans(without)-Latin origin (Madary, Joseph) 2. RE: C =Con(with) S means Sans(without)-Latin origin (Bonner, Janet) 3. ICC problem (Karla Arrington) 4. Bone Marrow Staining (Karla Arrington) 5. Re: Bone Marrow Staining (Rene J Buesa) 6. Re: IHC Background Staining (Victoria Baker) 7. non specific staining (Teisha Robertson) 8. RE: RE: Latin - Thank goodness! (Ingles Claire) 9. RE: RE: [Histonet] I need more ammunition (Thomas Jasper) 10. Method validation (Oto, Carrie) 11. Re: C =Con(with) S means Sans(without)-Latin origin (Joe Nocito) 12. Re: 4% paraformaldehyde all over again (John Kiernan) 13. thanks (louise renton) 14. RE: 4% paraformaldehyde all over again (Smith, Allen) 15. Catalase Staining for Liver Aspirates Help Please (Amy Porter) 16. Amended reports- Cerner CoPath users (Angela Bitting) 17. Standardized Microtomes (Nancy Schmitt) 18. Apipophilin and MCM (Michele Wich) 19. rubber stoppers & tubing (Atoska Gentry) 20. RE: Standardized Microtomes (Liz Chlipala) 21. Re: Standardized Microtomes (Jackie M O'Connor) 22. Paraffin thickness standard or measurement? (amy rizzo) 23. RE: Standardized Microtomes (Joyce Cline) ---------------------------------------------------------------------- Message: 1 Date: Mon, 12 May 2008 13:08:59 -0400 From: "Madary, Joseph" Subject: [Histonet] C =Con(with) S means Sans(without)-Latin origin To: Message-ID: Content-Type: text/plain; charset="us-ascii" Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Laboratory One Medimmune Way Gaithersburg, MD 20878 ph 301.398.4745/6360 fx 301.398.9745 To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. ------------------------------ Message: 2 Date: Mon, 12 May 2008 13:19:14 -0400 From: "Bonner, Janet" Subject: RE: [Histonet] C =Con(with) S means Sans(without)-Latin origin To: "Madary, Joseph" , histonet@lists.utsouthwestern.edu Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2647@fhosxchmb006.ADVENTISTCORP.NET> Content-Type: text/plain; charset=iso-8859-1 OK, I use w/ = with, and w/o = without. -American ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Madary, Joseph Sent: Mon 5/12/2008 1:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] C =Con(with) S means Sans(without)-Latin origin Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Laboratory One Medimmune Way Gaithersburg, MD 20878 ph 301.398.4745/6360 fx 301.398.9745 To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ============================================================================== The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ============================================================================== ------------------------------ Message: 3 Date: Mon, 12 May 2008 12:56:58 -0700 (PDT) From: Karla Arrington Subject: [Histonet] ICC problem To: histonet@lists.utsouthwestern.edu Message-ID: <251789.53346.qm@web32506.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello all.. I currently perform IHC's by hand.? Although they are beautiful, I do get a faint background staining inside the circle of the hydropholic pen.? It looks like excess DAB stain. I do not wipe around the tissues causing no static.? Any suggestions on how to fix this? Karla Arrington ________________________________________________________________________________ ____ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ ------------------------------ Message: 4 Date: Mon, 12 May 2008 13:04:05 -0700 (PDT) From: Karla Arrington Subject: [Histonet] Bone Marrow Staining To: histonet@lists.utsouthwestern.edu Message-ID: <92592.51349.qm@web32502.mail.mud.yahoo.com> Content-Type: text/plain; charset=us-ascii I currently use 10% NBF for fixing bone marrows (core and clot). Although lately the sections appear "washed out". I previously used B-5 Fixative. Is there a better fixative for fixing bone marrows so their cells are crisp and clear? Karla Arrington freckles9660@yahoo.com ________________________________________________________________________________ ____ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ ------------------------------ Message: 5 Date: Mon, 12 May 2008 13:17:13 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Bone Marrow Staining To: Karla Arrington , histonet@lists.utsouthwestern.edu Message-ID: <604837.79006.qm@web65710.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 The "washed out" appearance most likely has nothing to do with fixation. Check if you are heating the sections BEFORE they are completely drained off. That can cause that artifact. The only thing you have to be aware of when using NBF to fix BM specimens is to control the pH on the staining solutions (specially the Giemsa). Ren? J. Karla Arrington wrote: I currently use 10% NBF for fixing bone marrows (core and clot). Although lately the sections appear "washed out". I previously used B-5 Fixative. Is there a better fixative for fixing bone marrows so their cells are crisp and clear? Karla Arrington freckles9660@yahoo.com ________________________________________________________________________________ ____ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. ------------------------------ Message: 6 Date: Mon, 12 May 2008 16:33:38 -0400 From: "Victoria Baker" Subject: Re: [Histonet] IHC Background Staining To: "Karla Arrington" Cc: histonet@lists.utsouthwestern.edu Message-ID: <4f016b690805121333m20c00719v77da5f74d5645c28@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 What everyone else is saying is true and you will need to check your procedure carefully. Manual staining is a technique that is difficult with some antibodies and especially when working with a large slide number. One of the things that I did was eliminate using a pap pen. I used 2 X 2 strips of parafilm that I cut in strips of 1 X 2 and layed them on the tissue/slide after the reagent has been applied. To remove I dipped the slides in buffer and the film would slide right off. Good luck. I did IHC manually for a long time and it is not always easy. Vikki On 5/9/08, Karla Arrington wrote: > I do Immuno's by hand and I am getting background DAB precipitate on the inside circle of my sections. > What is causing this and how do I get rid of it? > > > ________________________________________________________________________________ ____ > Be a better friend, newshound, and > know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 7 Date: Mon, 12 May 2008 14:27:43 -0700 (PDT) From: Teisha Robertson Subject: [Histonet] non specific staining To: histonet@lists.utsouthwestern.edu Message-ID: <605614.77385.qm@web62515.mail.re1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 how do you eliminate non specific staining in olfactory bulb sections? --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. ------------------------------ Message: 8 Date: Mon, 12 May 2008 16:39:43 -0500 From: "Ingles Claire" Subject: RE: [Histonet] RE: Latin - Thank goodness! Cc: Message-ID: <08A0A863637F1349BBFD83A96B27A50A120128@uwhis-xchng3.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="iso-8859-1" I'm a youngster, but I also bought a latin dictionary just for the medical terminology aspect. Good thing I didn't have to take it. It is interesting stuff, but I'm terrible at learning languages. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Smith, Allen Sent: Mon 5/12/2008 11:51 AM To: 'Breeden, Sara' Cc: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Latin - Thank goodness! Latin is also useful in law. (A sponge left in the pelvic cavity is a case of "Res ipsa loquitur.") Latin also helps in puzzling out articles in Spanish or Portuguese. Most of all Latin gives access to a great literature that has been rather poorly translated. No translation of the Aeneid give one any conception of the magnificence of the original. ------------------------------ Message: 9 Date: Mon, 12 May 2008 15:52:06 -0700 From: "Thomas Jasper" Subject: RE: RE: [Histonet] I need more ammunition To: "WAYNE HOLLAND" Cc: histonet@lists.utsouthwestern.edu Message-ID: <90354A475B420441B2A0396E5008D4965E20AF@copc-sbs.COPC.local> Content-Type: text/plain; charset="US-ASCII" Hi Wayne, I know I already sent you a reply on this topic (off-line). Just had one more thought...you might want to point out to the "powers that be" that your organization is running a HUGE risk for the ULTIMATE in legal nightmares. Nothing gets the attention of "higher ups" quicker than $$$$$$'s and lawsuits. In the end Wayne, if you do not get anywhere with this group, I would seriously consider seeking employment elsewhere. That's not always the most desirable move, but let's face it...you are currently in a position and a market, which makes you a hot commodity. Secondly, you don't need to be implicated in any legal entanglements due to poor decisions (or lack of them) by the folks in authority where you work. Watch out for yourself man it's tough to speak truth to power. I pity the poor patients as well. Good luck, Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WAYNE HOLLAND Sent: Saturday, May 10, 2008 5:31 PM To: Histonet@lists.utsouthwestern.edu Subject: FW: RE: [Histonet] I need more ammunition I need more ammunition, please!! >From: WAYNE HOLLAND >Sent: Fri 5/9/2008 1:47 AM >To: Histonet@lists.utsouthwestern.edu >Subject: [Histonet] I need your help > > >Everyone, I have started a new job. I have many things that need fixed. >I have a gross room that are using regular cassettes metal tops and >they are wrapping all of the derm work in yes wet lens paper and >leaving them on the countertop by the hoods for periods of up to 45 >minutes. I know this is not good for obvious reasons. I need your >comments asap to help make them understand, again for obvious reasons. >I have been doing this for 28 years and I need other field associates >to back me up. Most of these specimen are very small. HELP! > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >NOTICE OF CONFIDENTIALITY >This electronic message, including attachments, is for the sole use of >the named recipient and may contain confidential or privileged >information protected by New York State, and Federal regulations. Any >unauthorized review, use, disclosure, copying or distribution is >strictly prohibited. If you are not the intended recipient or have >received this communication in error please contact the sender or >email.security@bassett.org and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Mon, 12 May 2008 16:26:06 -0700 From: "Oto, Carrie" Subject: [Histonet] Method validation To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <00EC53C1C120524B80A74AC951675233187E2C@CB-LIS-APSVR-1.ucsfmedicalcenter.org> Content-Type: text/plain Our Histology lab will be moving from one campus to another, 2 seperate CLIA's. Accrediting agency is Joint Commission. What, if any, has other labs done for method validation of histology equipment and reagents? For example, processor, stainer, IPOX stains, etc. Any and all information is appreciated especially if there is specific information for California labs. ------------------------------ Message: 11 Date: Mon, 12 May 2008 20:56:18 -0500 From: "Joe Nocito" Subject: Re: [Histonet] C =Con(with) S means Sans(without)-Latin origin To: "Bonner, Janet" , "Madary, Joseph" , Message-ID: <006b01c8b49c$898769b0$0302a8c0@yourxhtr8hvc4p> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original yeah, you go girl JTT ----- Original Message ----- From: "Bonner, Janet" To: "Madary, Joseph" ; Sent: Monday, May 12, 2008 12:19 PM Subject: RE: [Histonet] C =Con(with) S means Sans(without)-Latin origin OK, I use w/ = with, and w/o = without. -American ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Madary, Joseph Sent: Mon 5/12/2008 1:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] C =Con(with) S means Sans(without)-Latin origin Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Laboratory One Medimmune Way Gaithersburg, MD 20878 ph 301.398.4745/6360 fx 301.398.9745 To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ============================================================================== The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Tue, 13 May 2008 02:37:16 -0400 From: John Kiernan Subject: Re: [Histonet] 4% paraformaldehyde all over again To: Jackie M O'Connor Cc: Histonet , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="us-ascii" There is no such thing as 4% paraformaldehyde. Nobody can sell or us nauseam. said, in 3 words, vomit". Latin is concise formaldehyde is a real solution, and solution is to heat 40 grammes of  litre of water, with an alkaline catalyst to sp hydrolysis. Paraformaldehyde exists only as a solid substan which is insoluble in water.  All this has been in all the textbooks and manuals for 40+ years.
 
Joh UWO
London, Canada
Original Message -----
From: <Jackie.O'Connor@abbott.com>< Monday, May 12, 2008 10:52
Subject: [Histo 4% paraformaldehyde all over again
To: Histonet < Histonet@lists.utsouthwestern.edu>, histonet-bounces@li sts.utsouthwestern.edu

> Will vendors who cont quantities < again, please. <
> Jackie O'Conn Labs
> _________ _______________________ 5F Histonet mail Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Tue, 13 May 2008 11:02:22 +0200 From: "louise renton" Subject: [Histonet] thanks To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 thanks to all who took the time to respond to my OT question. Now i can sleep nights.......... -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ------------------------------ Message: 14 Date: Tue, 13 May 2008 09:07:41 -0400 From: "Smith, Allen" Subject: RE: [Histonet] 4% paraformaldehyde all over again To: 'John Kiernan' Cc: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" It appears that Dr. Kiernan is another victim of Microsoft office 2007. Could a users' coalition large enough to boycott it effectively be formed? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Kiernan Sent: Tuesday, May 13, 2008 2:37 AM To: Jackie M O'Connor Cc: Histonet; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] 4% paraformaldehyde all over again There is no such thing as 4% paraformaldehyde. Nobody can sell or us=uch a non-substance!
See Histonet archives passim ad nauseam.=6nbsp; (That's how the ancient Romans said, in 3 words,=2on and on, until you want to vomit". Latin is concise=
 
4% formaldehyde is a real solution, and =e of the ways of making the solution is to heat 40 grammes of =raformaldehyde in a litre of water, with an alkaline catalyst to sp?d up the hydrolysis. Paraformaldehyde exists only as a solid substan?, which is insoluble in water.  All this has been in all the textbooks and manuals for 40+ years.
 
Joh=iernan
Anatomy,  UWO
London, Canada
=D = = = =
----- Original Message -----
From: =ckie M O'Connor <Jackie.O'Connor@abbott.com><=>Date: Monday, May 12, 2008 10:52
Subject: [Histo=t] 4% paraformaldehyde all over again
To: Histonet < Histonet@lists.utsouthwestern.edu>, histonet-bounces@li sts.utsouthwestern.edu

> Will vendors who cont?ted me off line regarding supplying vast
> quantities <=>> of 4% paraformaldehyde contact me again, please. <=>> Thanks.
>
> Jackie O'Conn=
> Abbott Labs
> _________ _______________________ 5F=F_____________
> Histonet mail=g list
> Histonet@lists.utsouthwestern.edu
=6gt; http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Tue, 13 May 2008 10:49:40 -0400 From: "Amy Porter" Subject: [Histonet] Catalase Staining for Liver Aspirates Help Please To: Cc: miyakaw2@msu.edu Message-ID: <001201c8b508$92a98c50$8e7a0923@histolab> Content-Type: text/plain; charset="iso-8859-1" Hello to all - I am looking for an enzyme staining method to demonstrate peroxisomes (catalase specifically) in Canine Liver aspirate smears. If anyone out there has anything they would be will to share it would be so appreciated. I have spent a great deal of time searching on the web and have not yet found a methodology. Thanks in advance for any assistance. Amy Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu ------------------------------ Message: 16 Date: Tue, 13 May 2008 11:23:17 -0400 From: "Angela Bitting" Subject: [Histonet] Amended reports- Cerner CoPath users To: Message-ID: <48297A25.2B7F.00C9.0@geisinger.edu> Content-Type: text/plain; charset="us-ascii" I've got a question for users of Cerner Copath. Do you have CoPath set up so that when a report is amended the original diagnosis remains visible along with the amended diagnosis? Also, is there some type of flag at the top of the report to alert a physician that there have been changes to the report? Thanks for your help on this one. Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD ------------------------------ Message: 17 Date: Tue, 13 May 2008 11:28:24 -0500 From: Nancy Schmitt Subject: [Histonet] Standardized Microtomes To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <9FC023A4AB52BB4D87DC6456081A822C087C8D@mercury.pa-ucl.com> Content-Type: text/plain; charset="iso-8859-1" Good Morning to all Does anybody standardize microtomes so they all cut at same angle? Is this impossible? We only cut control blocks on one microtome because they are each at a little different angle. Would be nice to be able to do at all........... Thanks for any feedback Nancy Schmitt Histology Coordinator Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ------------------------------ Message: 18 Date: Tue, 13 May 2008 11:30:13 -0500 From: "Michele Wich" Subject: [Histonet] Apipophilin and MCM To: Message-ID: <62A8156F8071C8439080D626DF8C33A602E3F5@wave-mail.7thwave.local> Content-Type: text/plain; charset="US-ASCII" Does anyone know if Apipophilin and MCM (mini chromosome maintenance protein) are commercially available and, if so, who sells them? Thanks for any info! This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer ------------------------------ Message: 19 Date: Tue, 13 May 2008 11:35:58 -0500 From: Atoska Gentry Subject: [Histonet] rubber stoppers & tubing To: Histonet Message-ID: <4829C36E.8000504@vetmed.auburn.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed hello, if any of you have a source for two-hole black rubber stoppers measuring : 21mm top x 13mm bottom x 7mm in length ( with the inscription 304w SET on top, and with one hole measuring 2-3mm and the other 3-4 mm); and or 15mm bottom x 8mm in length; also, rigid plastic tubing 2-3 mm in diameter, 196mm in length will you please contact me ASAP? Thank you kindly, Atoska -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu ------------------------------ Message: 20 Date: Tue, 13 May 2008 10:38:18 -0600 From: "Liz Chlipala" Subject: RE: [Histonet] Standardized Microtomes To: "Nancy Schmitt" , Message-ID: Content-Type: text/plain; charset="US-ASCII" Newcomer supply has a device that will standardize microtomes. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Tuesday, May 13, 2008 10:28 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Standardized Microtomes Good Morning to all Does anybody standardize microtomes so they all cut at same angle? Is this impossible? We only cut control blocks on one microtome because they are each at a little different angle. Would be nice to be able to do at all........... Thanks for any feedback Nancy Schmitt Histology Coordinator Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Tue, 13 May 2008 11:41:10 -0500 From: Jackie M O'Connor Subject: Re: [Histonet] Standardized Microtomes To: Nancy Schmitt Cc: "'histonet@lists.utsouthwestern.edu'" , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Get a block aligner. We have aligned all of our microtomes so that any technicican can do a recut no matter what machine it was originally cut on. Nancy Schmitt Sent by: histonet-bounces@lists.utsouthwestern.edu 05/13/2008 11:28 AM To "'histonet@lists.utsouthwestern.edu'" cc Subject [Histonet] Standardized Microtomes Good Morning to all Does anybody standardize microtomes so they all cut at same angle? Is this impossible? We only cut control blocks on one microtome because they are each at a little different angle. Would be nice to be able to do at all........... Thanks for any feedback Nancy Schmitt Histology Coordinator Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 22 Date: Tue, 13 May 2008 11:54:13 -0500 From: amy rizzo Subject: [Histonet] Paraffin thickness standard or measurement? To: Message-ID: <000001c8b519$f8d86440$c91e3086@marqnet.mu.edu> Content-Type: text/plain; charset="us-ascii" Is there anyway to measure the thickness of your paraffin section as it comes off a microtome? I have noticed mine isn't as consistent as I would like. Do they sell something anywhere that does this? Thanks, Amy Rizzo ------------------------------ Message: 23 Date: Tue, 13 May 2008 12:56:55 -0400 From: "Joyce Cline" Subject: RE: [Histonet] Standardized Microtomes To: Message-ID: <001201c8b51a$5996a080$1d2a14ac@wchsys.org> Content-Type: text/plain; charset="us-ascii" Each week we align all our microtomes. A company called Advance Innovations has created an aligner that is available through several companies. Aligning our microtomes prevents losing tissue from small biopsies when a recut is called for. ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Good Morning to all Does anybody standardize microtomes so they all cut at same angle? Is this impossible? We only cut control blocks on one microtome because they are each at a little different angle. Would be nice to be able to do at all........... Thanks for any feedback Nancy Schmitt Histology Coordinator Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 54, Issue 17 **************************************** The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From es144131 <@t> bcm.tmc.edu Tue May 13 14:13:00 2008 From: es144131 <@t> bcm.tmc.edu (Stephens, Elizabeth Humes) Date: Tue May 13 14:13:05 2008 Subject: [Histonet] slide scanner Message-ID: Does anybody have a recommendation for an inexpensive but reliable histology slide scanner? We have the Microtek Artixscan 4000tf and use the Microtek ScanWizard Pro TX software. The machine works fine but the software keeps crashing. We downloaded the most recent version (Scanwizard Pro TX7) but still am having problems on both Mac and PC, lab and personal computers. Thanks for your advice!! Elizabeth Stephens From rjbuesa <@t> yahoo.com Tue May 13 14:30:06 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue May 13 14:30:10 2008 Subject: [Histonet] salary scales In-Reply-To: <8CA833536AA84EE-B60-1EFF@FWM-M44.sysops.aol.com> Message-ID: <927545.19479.qm@web65712.mail.ac4.yahoo.com> Add, at least, $5.00/hour to each category and you will be competitive. Ren? J. godsgalnow@aol.com wrote: I know that this is a sore subject, but typically at what part of the range do you brings histotechnologists in at?? And if you wouldn't mind sharing the range I would be grateful.? I work for a company that is run by business people not lab people and I am trying to convince them to adjust the salaries.? For instance, our histotechnician range is 17.96 - 21.45, our histotechnologist range is 21.14 - 26.58, and our lab supervisor range is 25.46 - 34.28. Thanks in advance, Roxanne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue May 13 14:49:51 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue May 13 14:49:54 2008 Subject: [Histonet] Paraffin thickness standard or measurement? In-Reply-To: <000001c8b519$f8d86440$c91e3086@marqnet.mu.edu> Message-ID: <373477.31608.qm@web65703.mail.ac4.yahoo.com> Amy: Since time immemorial section thickness determination has been an almost unattainable goal. The now defunct American Optical Company (manufacturers of the Spencer microtomes) developed a method based on an specially designed interferometer that allowed the thickness determination. They also designed a resistance to cutting method, but even they thought neither was practical in the routine laboratory. I used to evaluate the thickness of the section using the lymphocytes' diameter (around 7 ?m) and if there were one or several lymphocytes layers as an indirect thickness method. Now if you are experiencing this problem it is better to check all the moving parts of your microtome and tight those that should be tight (including the block and blades holders) in order to avoid vibrations that are usually the cause of successive thick-thin sections. Hope this will help you Ren? J. amy rizzo wrote: _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charles.Embrey <@t> carle.com Tue May 13 15:21:54 2008 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Tue May 13 15:22:00 2008 Subject: [Histonet] salary scales In-Reply-To: <927545.19479.qm@web65712.mail.ac4.yahoo.com> References: <8CA833536AA84EE-B60-1EFF@FWM-M44.sysops.aol.com> <927545.19479.qm@web65712.mail.ac4.yahoo.com> Message-ID: <44780C571F28624DBB446DE55C4D733A1FE5BA@EXCHANGEBE1.carle.com> Isn't that a rather broad statement, Ren?? Without knowing what part of the country we are talking about it is hard to make an educated guess as to salary range. Roxanne's figures are actually slightly higher than the typical pay here in the Midwest (Illinois) for technician and a great deal higher for the other two categories. Here in Illinois she would be very completive. Chuck -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, May 13, 2008 2:30 PM To: godsgalnow@aol.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] salary scales Add, at least, $5.00/hour to each category and you will be competitive. Ren? J. godsgalnow@aol.com wrote: I know that this is a sore subject, but typically at what part of the range do you brings histotechnologists in at?? And if you wouldn't mind sharing the range I would be grateful.? I work for a company that is run by business people not lab people and I am trying to convince them to adjust the salaries.? For instance, our histotechnician range is 17.96 - 21.45, our histotechnologist range is 21.14 - 26.58, and our lab supervisor range is 25.46 - 34.28. Thanks in advance, Roxanne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sjchtascp <@t> yahoo.com Tue May 13 15:25:54 2008 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Tue May 13 15:25:57 2008 Subject: [Histonet] Milwaukee, WI Message-ID: <725849.35508.qm@web38203.mail.mud.yahoo.com> I use to work in the Milwaukee, WI area as a contract HT. CLS, the agency I worked for is no longer in business. I see theres several HT positions in the Milwaukee area. Does anyone know of a staffing agency that covers that area. Thanks, Steve From rjbuesa <@t> yahoo.com Tue May 13 16:16:31 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue May 13 16:16:35 2008 Subject: [Histonet] salary scales In-Reply-To: <44780C571F28624DBB446DE55C4D733A1FE5BA@EXCHANGEBE1.carle.com> Message-ID: <9807.6588.qm@web65710.mail.ac4.yahoo.com> Charles: No, I don't think it is "a rather broad statement". Regardless of the area of the country, you should never negotiate a salary as part of a group. You are "selling" your personal abilities and trying to cover your family needs. How different is the gasoline price today by regions? Are they so different? How about food products?. I encourage you to read my article in Advance for MLP (Jan.28, 2008) where I demonstrate that all the "alleged" salary regional variations are non statistically significant and offer some advise in how to negotiate your salary. Ren? J. "Charles.Embrey" wrote: From jstaruk <@t> masshistology.com Tue May 13 17:46:51 2008 From: jstaruk <@t> masshistology.com (jstaruk) Date: Tue May 13 17:46:58 2008 Subject: [Histonet] salary scales In-Reply-To: <9807.6588.qm@web65710.mail.ac4.yahoo.com> Message-ID: <0E8C20BD05BC44B9BE7B2C9E16CF4FC6@JimPC> As president and owner of a histology lab (although this can be any business), I feel I must add my thoughts to this discussion on salary (which pops up on this newsgroup quite often). First of all, there's more to a "salary" that what the number is on your weekly pay stub. Who's paying for your health insurance? Does your company offer a matching 401K plan? Life insurance? How many weeks paid vacation do you get? How many paid holidays? Paid sick days? Can you leave when your job is complete (and still get paid for the rest of the day)? Does your company pay for your lunch hour? Do they pay for your lunch? Do they offer child care? A work-out room? There's a heck of a lot more many companies do for their employees than simply paying them a certain rate for every hour they are "punched in". I figure any employee who works at my company for "only" $25.00 per hour is actually making about $55.00 per hour if they utilize all (or at least most) of the benefits we offer them. Then there's the matching social security, worker's Comp. insurance, state employee taxes and so on that we're also responsible to pay for each employee. Think of all this next time you say you only make $25.00 per hour! Jim _____________________ Jim Staruk Mass Histology Service www.masshistology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, May 13, 2008 5:17 PM To: Charles.Embrey; godsgalnow@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] salary scales Charles: No, I don't think it is "a rather broad statement". Regardless of the area of the country, you should never negotiate a salary as part of a group. You are "selling" your personal abilities and trying to cover your family needs. How different is the gasoline price today by regions? Are they so different? How about food products?. I encourage you to read my article in Advance for MLP (Jan.28, 2008) where I demonstrate that all the "alleged" salary regional variations are non statistically significant and offer some advise in how to negotiate your salary. Ren? J. "Charles.Embrey" wrote: _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dshowers <@t> dermpathdiagnostics.com Tue May 13 17:58:19 2008 From: dshowers <@t> dermpathdiagnostics.com (Showers, Doug) Date: Tue May 13 17:58:23 2008 Subject: [Histonet] salary scales References: <0E8C20BD05BC44B9BE7B2C9E16CF4FC6@JimPC> Message-ID: I agree that the prospective employee needs to weigh all of the other benefits, but frankly most do not. The cost of benefits to the employer is usually of little or no interest to the employee. Most simply want to know how much they will be taking home after taxes, insurance, etc. I have interviewed a lot of job applicants over the years and most of them give the benefits a cursory glance. I do think that if you offer great benefits your current employees spread the good word and attract more applicants for your positions. Often, during the years I spent in the Air Force, I would get a mailing from the military laying out the dollar cost of all of my benefits and I remember thinking "If I could get a little more of that in my paycheck I might be a happier person." Doug Showers MS, HT (ASCP) ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of jstaruk Sent: Tue 5/13/2008 5:46 PM To: 'Rene J Buesa'; 'Charles.Embrey'; godsgalnow@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] salary scales As president and owner of a histology lab (although this can be any business), I feel I must add my thoughts to this discussion on salary (which pops up on this newsgroup quite often). First of all, there's more to a "salary" that what the number is on your weekly pay stub. Who's paying for your health insurance? Does your company offer a matching 401K plan? Life insurance? How many weeks paid vacation do you get? How many paid holidays? Paid sick days? Can you leave when your job is complete (and still get paid for the rest of the day)? Does your company pay for your lunch hour? Do they pay for your lunch? Do they offer child care? A work-out room? There's a heck of a lot more many companies do for their employees than simply paying them a certain rate for every hour they are "punched in". I figure any employee who works at my company for "only" $25.00 per hour is actually making about $55.00 per hour if they utilize all (or at least most) of the benefits we offer them. Then there's the matching social security, worker's Comp. insurance, state employee taxes and so on that we're also responsible to pay for each employee. Think of all this next time you say you only make $25.00 per hour! Jim _____________________ Jim Staruk Mass Histology Service www.masshistology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, May 13, 2008 5:17 PM To: Charles.Embrey; godsgalnow@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] salary scales Charles: No, I don't think it is "a rather broad statement". Regardless of the area of the country, you should never negotiate a salary as part of a group. You are "selling" your personal abilities and trying to cover your family needs. How different is the gasoline price today by regions? Are they so different? How about food products?. I encourage you to read my article in Advance for MLP (Jan.28, 2008) where I demonstrate that all the "alleged" salary regional variations are non statistically significant and offer some advise in how to negotiate your salary. Ren? J. "Charles.Embrey" wrote: _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Tue May 13 18:58:34 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue May 13 18:58:38 2008 Subject: [Histonet] M$ office glitches Message-ID: <582736990805131658k726ad38eu4a2519b88742eb38@mail.gmail.com> Hi, Open Office ... Just as good and FREE!! Let's not be lemmings and throw our money at Micro$oft products just because they are there. They are NOT the only product out there! Open Source Rules! Amos Brooks Message: 14 Date: Tue, 13 May 2008 09:07:41 -0400 From: "Smith, Allen" Subject: RE: [Histonet] 4% paraformaldehyde all over again To: 'John Kiernan' Cc: "'histonet@lists.utsouthwestern.edu'" Message-ID: < E4132130AC2F764D8C173C5400D530426748D14E9E@exchsrv02.barrynet.barry.edu> Content-Type: text/plain; charset="us-ascii" It appears that Dr. Kiernan is another victim of Microsoft office 2007. Could a users' coalition large enough to boycott it effectively be formed? From amosbrooks <@t> gmail.com Tue May 13 19:10:55 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue May 13 19:11:00 2008 Subject: [Histonet] Standardized Microtomes Message-ID: <582736990805131710s398184e7oaa2d5d3ae40e4b43@mail.gmail.com> Hi Nancy, We do not do this, because one of the first things one should learn when learning how to cut is how to change the angle of whatever microtome you are cutting at. Setting them all to the same angle just makes learning how to use those funny little knobs on the top of the block holder pointless. So when a situation arises theat requires you to change the angle no one knows how to do it. The solution, just cut into it and the block will be the same angle, or re-embed the tissue (astonishingly lazy- extra work to accomplish nothing) and trim it back to the angle that is on all the microtomes thereby loosing tissue anyway! A far better solution, teach techs to adjust the block holder without loosing tissue (advance slowly)! Give a man a fish & he'll eat for a day. Teach a man to fish and he'll never go hungry. Just my $0.02 Amos Brooks Message: 17 Date: Tue, 13 May 2008 11:28:24 -0500 From: Nancy Schmitt Subject: [Histonet] Standardized Microtomes To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <9FC023A4AB52BB4D87DC6456081A822C087C8D@mercury.pa-ucl.com> Content-Type: text/plain; charset="iso-8859-1" Good Morning to all Does anybody standardize microtomes so they all cut at same angle? Is this impossible? We only cut control blocks on one microtome because they are each at a little different angle. Would be nice to be able to do at all........... Thanks for any feedback Nancy Schmitt Histology Coordinator Dubuque, IA From MichelM9 <@t> chw.edu.au Tue May 13 20:38:20 2008 From: MichelM9 <@t> chw.edu.au (Michelle McDonald) Date: Tue May 13 20:38:32 2008 Subject: [Histonet] Aqueous mountant Message-ID: <47BD6E7614A693499453835D47E36F7004421B29@hedwig.nch.kids> Hi all, Does anyone have a good aqueous mountant they can recommend? We are having trouble with ours, lots of bubbles we cannot get rid of!! Thanks in advance Michelle Dr Michelle McDonald B.MedSci, PhD Research Officer Orthopaedic Research Dept. The Children's Hospital Westmead. Westmead NSW 2145 Australia Ph. +612 9845 1451 Fax. +612 9845 3078 ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From Barry.R.Rittman <@t> uth.tmc.edu Tue May 13 20:51:33 2008 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Tue May 13 20:53:58 2008 Subject: [Histonet] Aqueous mountant References: <47BD6E7614A693499453835D47E36F7004421B29@hedwig.nch.kids> Message-ID: Michelle Have you tried to ultrasonicate the aqueous mountant that you are currently using? This quickly brings bubbles to the surface - we have used this for several things including glycerin mountants. Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Michelle McDonald Sent: Tue 5/13/2008 8:38 PM To: histonet@lists.utsouthwestern.edu Cc: Renjing Liu Subject: [Histonet] Aqueous mountant Hi all, Does anyone have a good aqueous mountant they can recommend? We are having trouble with ours, lots of bubbles we cannot get rid of!! Thanks in advance Michelle Dr Michelle McDonald B.MedSci, PhD Research Officer Orthopaedic Research Dept. The Children's Hospital Westmead. Westmead NSW 2145 Australia Ph. +612 9845 1451 Fax. +612 9845 3078 ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jorge.tornero <@t> gmail.com Wed May 14 01:09:13 2008 From: jorge.tornero <@t> gmail.com (Jorge Tornero) Date: Wed May 14 01:09:23 2008 Subject: [Histonet] About phosphate buffer - simple (silly?) question Message-ID: <8c964a790805132309j307b752cnac77a26637aaa468@mail.gmail.com> Hi, I have to prepair phosphate buffered formaline to preserve anchovy gonads. I am pretty new in this bussines, and unfortunately my knowledge on chemistry is far from ideal. When I started my work some time ago, I "inherited" some formulas about the matter, but soon I've realized that those formulas are imprecise and vague: for instance, they just say "mix x grams of dibasic salt with y grams of monobasic salt", and those x's and y's vary from one formula to other. Of course, I can't tell how many hydration water molecules are considered for the chemical species in the formulas, and of course there is nothing about the purity of the salts used. So I've decided to try to make the calculations by myself, thus solving the problem for every time I need to make this buffer or other. So I have been looking around and found the Henderson-Hasselbalch equation and I've employed it to make calculations, and everything is ok, BUT... The dissociation constant for phosphate, pKa, seems to be in most cases 7.2, but I've found, in several places, to be 6.86. They say it is a apparent pKa (they call it pKa') due to the ionic strenght of the solution. The problem is: Which pKa should I use? I guess the correct is 6.86, but I don't know the reasons for it. I've read that pKa'=pKa+correction factor, calculations involving ionic strenght but I am not able to find the tables where the correction factor for a given ionic strenght are tabulated. So my quesion is: Which pKa should I use? Why? How to calculate that pKa? I hope my bad english is not a problem for you to understand me. Best Regards, Jorge Tornero IEO-C?diz Spain From annigyg <@t> gmail.com Wed May 14 01:21:57 2008 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Wed May 14 01:22:02 2008 Subject: [Histonet] About phosphate buffer - simple (silly?) question In-Reply-To: <8c964a790805132309j307b752cnac77a26637aaa468@mail.gmail.com> References: <8c964a790805132309j307b752cnac77a26637aaa468@mail.gmail.com> Message-ID: Hi Jorge google 'neutral buffered formalin' and you will have the recipe for NBF Anne 2008/5/14 Jorge Tornero : > Hi, > > I have to prepair phosphate buffered formaline to preserve anchovy gonads. > I > am pretty new in this bussines, and unfortunately my knowledge on > chemistry > is far from ideal. > > When I started my work some time ago, I "inherited" some formulas about > the > matter, but soon I've realized that those formulas are imprecise and > vague: > for instance, they just say "mix x grams of dibasic salt with y grams of > monobasic salt", and those x's and y's vary from one formula to other. Of > course, I can't tell how many hydration water molecules are considered for > the chemical species in the formulas, and of course there is nothing about > the purity of the salts used. So I've decided to try to make the > calculations by myself, thus solving the problem for every time I need to > make this buffer or other. > > So I have been looking around and found the Henderson-Hasselbalch equation > and I've employed it to make calculations, and everything is ok, BUT... > > The dissociation constant for phosphate, pKa, seems to be in most cases > 7.2, > but I've found, in several places, to be 6.86. They say it is a apparent > pKa > (they call it pKa') due to the ionic strenght of the solution. The problem > is: Which pKa should I use? I guess the correct is 6.86, but I don't know > the reasons for it. I've read that pKa'=pKa+correction factor, > calculations > involving ionic strenght but I am not able to find the tables where the > correction factor for a given ionic strenght are tabulated. > > So my quesion is: Which pKa should I use? Why? How to calculate that pKa? > > I hope my bad english is not a problem for you to understand me. > > Best Regards, > > Jorge Tornero > IEO-C?diz > Spain > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From jorge.tornero <@t> gmail.com Wed May 14 01:40:59 2008 From: jorge.tornero <@t> gmail.com (Jorge Tornero) Date: Wed May 14 01:41:04 2008 Subject: [Histonet] About phosphate buffer - simple (silly?) question In-Reply-To: References: <8c964a790805132309j307b752cnac77a26637aaa468@mail.gmail.com> Message-ID: <8c964a790805132340j74003539jff138257f7d7897d@mail.gmail.com> Hi Ann and All, I know about those recipes, but I would rather prefer to be able to calculate it by myself and solve my problem "for ever" Thank you very much anyway Jorge Tornero IEO-C?diz Spain 2008/5/14 Anne van Binsbergen : > Hi Jorge > google 'neutral buffered formalin' and you will have the recipe for NBF > Anne > > 2008/5/14 Jorge Tornero : > > > Hi, > > > > I have to prepair phosphate buffered formaline to preserve anchovy > > gonads. I > > am pretty new in this bussines, and unfortunately my knowledge on > > chemistry > > is far from ideal. > > > > When I started my work some time ago, I "inherited" some formulas about > > the > > matter, but soon I've realized that those formulas are imprecise and > > vague: > > for instance, they just say "mix x grams of dibasic salt with y grams of > > monobasic salt", and those x's and y's vary from one formula to other. > > Of > > course, I can't tell how many hydration water molecules are considered > > for > > the chemical species in the formulas, and of course there is nothing > > about > > the purity of the salts used. So I've decided to try to make the > > calculations by myself, thus solving the problem for every time I need > > to > > make this buffer or other. > > > > So I have been looking around and found the Henderson-Hasselbalch > > equation > > and I've employed it to make calculations, and everything is ok, BUT... > > > > The dissociation constant for phosphate, pKa, seems to be in most cases > > 7.2, > > but I've found, in several places, to be 6.86. They say it is a apparent > > pKa > > (they call it pKa') due to the ionic strenght of the solution. The > > problem > > is: Which pKa should I use? I guess the correct is 6.86, but I don't > > know > > the reasons for it. I've read that pKa'=pKa+correction factor, > > calculations > > involving ionic strenght but I am not able to find the tables where the > > correction factor for a given ionic strenght are tabulated. > > > > So my quesion is: Which pKa should I use? Why? How to calculate that > > pKa? > > > > I hope my bad english is not a problem for you to understand me. > > > > Best Regards, > > > > Jorge Tornero > > IEO-C?diz > > Spain > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Anne van Binsbergen (Hope) > Abu Dhabi > UAE From ian.montgomery <@t> bio.gla.ac.uk Wed May 14 03:53:30 2008 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Wed May 14 03:53:37 2008 Subject: FW: [Histonet] Aqueous mountant Message-ID: <45E8ABB6BDAB461BBDB2A9EDD7598AAE@IBLS.GLA.AC.UK> Michelle, Have you tried aquamount from VWR? Been using it for years without problem. Ian. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michelle McDonald Sent: 14 May 2008 02:38 To: histonet@lists.utsouthwestern.edu Cc: Renjing Liu Subject: [Histonet] Aqueous mountant Hi all, Does anyone have a good aqueous mountant they can recommend? We are having trouble with ours, lots of bubbles we cannot get rid of!! Thanks in advance Michelle Dr Michelle McDonald B.MedSci, PhD Research Officer Orthopaedic Research Dept. The Children's Hospital Westmead. Westmead NSW 2145 Australia Ph. +612 9845 1451 Fax. +612 9845 3078 ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Wed May 14 06:01:05 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed May 14 06:01:13 2008 Subject: [Histonet] Sakura VIP flood? References: Message-ID: <004801c8b5b1$d08ab810$0302a8c0@yourxhtr8hvc4p> Gee Teri, that's a first for me. I've used the VIP 5 for years and never had this happen. I know, I've been a lot of help. JTT ----- Original Message ----- From: "Johnson, Teri" To: Sent: Tuesday, May 13, 2008 12:46 PM Subject: [Histonet] Sakura VIP flood? Any of you users of the Sakura VIP 5 have any problems with the fume control water somehow siphoning and flooding into the guts of the instrument? The manual tells us to put between 2-3 liters of water in there. We had issues with this happening early on, and were told we had too much water in there. We now use much less, and until 3 days ago had no problem with it. This morning came in to water all in the overflow tray, dripping down from the inside of the instrument on to the floor, and an empty fume control water bottle. My in house service guy will be calling Sakura today, but wanted to know of others' experiences with this issue. I'm not interested in bashing the product, it's worked well for us for over 6 years. Just wondering if it's happened to anybody else. Thanks! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From annigyg <@t> gmail.com Wed May 14 06:10:32 2008 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Wed May 14 06:10:40 2008 Subject: [Histonet] Sakura VIP flood? In-Reply-To: <004801c8b5b1$d08ab810$0302a8c0@yourxhtr8hvc4p> References: <004801c8b5b1$d08ab810$0302a8c0@yourxhtr8hvc4p> Message-ID: WOW!! am very curious to know what Sakura tells you also have a 'trusty VIP5 workhorse' in my stable - but never one that has flooded!! keep us in the loop Annie 2008/5/14 Joe Nocito : > Gee Teri, > that's a first for me. I've used the VIP 5 for years and never had this > happen. I know, I've been a lot of help. > > JTT > ----- Original Message ----- From: "Johnson, Teri" > > To: > Sent: Tuesday, May 13, 2008 12:46 PM > Subject: [Histonet] Sakura VIP flood? > > > Any of you users of the Sakura VIP 5 have any problems with the fume > control water somehow siphoning and flooding into the guts of the > instrument? The manual tells us to put between 2-3 liters of water in there. > We had issues with this happening early on, and were told we had too much > water in there. We now use much less, and until 3 days ago had no problem > with it. This morning came in to water all in the overflow tray, dripping > down from the inside of the instrument on to the floor, and an empty fume > control water bottle. > > My in house service guy will be calling Sakura today, but wanted to know of > others' experiences with this issue. I'm not interested in bashing the > product, it's worked well for us for over 6 years. Just wondering if it's > happened to anybody else. > > Thanks! > > Teri Johnson, HT(ASCP)QIHC > Managing Director Histology Facility > Stowers Institute for Medical Research > 1000 E. 50th St. > Kansas City, MO 64110 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From jqb7 <@t> cdc.gov Wed May 14 06:36:06 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed May 14 06:36:54 2008 Subject: [Histonet] Sakura VIP flood? In-Reply-To: References: <004801c8b5b1$d08ab810$0302a8c0@yourxhtr8hvc4p> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A747A34C@LTA3VS011.ees.hhs.gov> If it was an issue of overfilling like Sakura said then more people would have experienced it because I am sure most of us fill to between 2-3 liters. I think it is something else, definitely! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne van Binsbergen Sent: Wednesday, May 14, 2008 7:11 AM To: Joe Nocito Cc: histonet@lists.utsouthwestern.edu; Johnson, Teri Subject: Re: [Histonet] Sakura VIP flood? WOW!! am very curious to know what Sakura tells you also have a 'trusty VIP5 workhorse' in my stable - but never one that has flooded!! keep us in the loop Annie 2008/5/14 Joe Nocito : > Gee Teri, > that's a first for me. I've used the VIP 5 for years and never had > this happen. I know, I've been a lot of help. > > JTT > ----- Original Message ----- From: "Johnson, Teri" > > To: > Sent: Tuesday, May 13, 2008 12:46 PM > Subject: [Histonet] Sakura VIP flood? > > > Any of you users of the Sakura VIP 5 have any problems with the fume > control water somehow siphoning and flooding into the guts of the > instrument? The manual tells us to put between 2-3 liters of water in there. > We had issues with this happening early on, and were told we had too > much water in there. We now use much less, and until 3 days ago had no > problem with it. This morning came in to water all in the overflow > tray, dripping down from the inside of the instrument on to the floor, > and an empty fume control water bottle. > > My in house service guy will be calling Sakura today, but wanted to > know of others' experiences with this issue. I'm not interested in > bashing the product, it's worked well for us for over 6 years. Just > wondering if it's happened to anybody else. > > Thanks! > > Teri Johnson, HT(ASCP)QIHC > Managing Director Histology Facility > Stowers Institute for Medical Research 1000 E. 50th St. > Kansas City, MO 64110 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jennifer.l.hofecker <@t> Vanderbilt.Edu Wed May 14 06:44:49 2008 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Wed May 14 06:44:56 2008 Subject: [Histonet] Standardized Microtomes In-Reply-To: <582736990805131710s398184e7oaa2d5d3ae40e4b43@mail.gmail.com> References: <582736990805131710s398184e7oaa2d5d3ae40e4b43@mail.gmail.com> Message-ID: <898D946569A27444B65667A49C0740520175B453@mailbe06.mc.vanderbilt.edu> Ok, I have donned my flame resistant suit... I agree 100% with Amos! I learned to angle the block when I learned to cut and I have worked in a few labs where that was "forbidden". It always seemed so silly to me that instead of taking 0.5 seconds to angle the block and get a perfect full face of the tissue that I had to spend 15 minutes re-embedding the block. I actually had to tape the adjustment knobs on my microtome so I wouldn't "break the rules". One might think that if you angle the block, how will the next tech be able to "line it up"? Answer: If everyone knows how to angle the block, it's not an issue. One other question: If we are never supposed to angle the block, why are those little knobs on the block holder? Why don't microtome manufacturers make them all "standard" and not adjustable? I choose to believe that we are highly skilled individuals and that patient care is best served by us producing quality work. I am "pro-angling" all the way! I know... I know... Have a great day everyone! Jennifer L. Hofecker HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph 615.343.0083 fax 615.343.7089 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Tuesday, May 13, 2008 7:11 PM To: nancy_schmitt@pa-ucl.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] Standardized Microtomes Hi Nancy, We do not do this, because one of the first things one should learn when learning how to cut is how to change the angle of whatever microtome you are cutting at. Setting them all to the same angle just makes learning how to use those funny little knobs on the top of the block holder pointless. So when a situation arises theat requires you to change the angle no one knows how to do it. The solution, just cut into it and the block will be the same angle, or re-embed the tissue (astonishingly lazy- extra work to accomplish nothing) and trim it back to the angle that is on all the microtomes thereby loosing tissue anyway! A far better solution, teach techs to adjust the block holder without loosing tissue (advance slowly)! Give a man a fish & he'll eat for a day. Teach a man to fish and he'll never go hungry. Just my $0.02 Amos Brooks Message: 17 Date: Tue, 13 May 2008 11:28:24 -0500 From: Nancy Schmitt Subject: [Histonet] Standardized Microtomes To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <9FC023A4AB52BB4D87DC6456081A822C087C8D@mercury.pa-ucl.com> Content-Type: text/plain; charset="iso-8859-1" Good Morning to all Does anybody standardize microtomes so they all cut at same angle? Is this impossible? We only cut control blocks on one microtome because they are each at a little different angle. Would be nice to be able to do at all........... Thanks for any feedback Nancy Schmitt Histology Coordinator Dubuque, IA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From annigyg <@t> gmail.com Wed May 14 06:45:44 2008 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Wed May 14 06:45:49 2008 Subject: [Histonet] Sakura VIP flood? In-Reply-To: <1CE1847DFEA0A647B1CCDE4108EA60A747A34C@LTA3VS011.ees.hhs.gov> References: <004801c8b5b1$d08ab810$0302a8c0@yourxhtr8hvc4p> <1CE1847DFEA0A647B1CCDE4108EA60A747A34C@LTA3VS011.ees.hhs.gov> Message-ID: overfilling should not be a problem for the VIP5 - we fill our cleaning and fume control bottles to just over 4.5 litres - the machine only pulls out what it needs clean/reposition the rubber gasket which seals the retort lid and check the rotary valve thats my 5cents worth 2008/5/14 Bartlett, Jeanine (CDC/CCID/NCZVED) : > If it was an issue of overfilling like Sakura said then more people > would have experienced it because I am sure most of us fill to between > 2-3 liters. I think it is something else, definitely! > > > Jeanine Bartlett > Infectious Diseases Pathology Branch > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne van > Binsbergen > Sent: Wednesday, May 14, 2008 7:11 AM > To: Joe Nocito > Cc: histonet@lists.utsouthwestern.edu; Johnson, Teri > Subject: Re: [Histonet] Sakura VIP flood? > > WOW!! > am very curious to know what Sakura tells you also have a 'trusty VIP5 > workhorse' in my stable - but never one that has flooded!! > keep us in the loop > Annie > > 2008/5/14 Joe Nocito : > > > Gee Teri, > > that's a first for me. I've used the VIP 5 for years and never had > > this happen. I know, I've been a lot of help. > > > > JTT > > ----- Original Message ----- From: "Johnson, Teri" > > > > To: > > Sent: Tuesday, May 13, 2008 12:46 PM > > Subject: [Histonet] Sakura VIP flood? > > > > > > Any of you users of the Sakura VIP 5 have any problems with the fume > > control water somehow siphoning and flooding into the guts of the > > instrument? The manual tells us to put between 2-3 liters of water in > there. > > We had issues with this happening early on, and were told we had too > > much water in there. We now use much less, and until 3 days ago had no > > > problem with it. This morning came in to water all in the overflow > > tray, dripping down from the inside of the instrument on to the floor, > > > and an empty fume control water bottle. > > > > My in house service guy will be calling Sakura today, but wanted to > > know of others' experiences with this issue. I'm not interested in > > bashing the product, it's worked well for us for over 6 years. Just > > wondering if it's happened to anybody else. > > > > Thanks! > > > > Teri Johnson, HT(ASCP)QIHC > > Managing Director Histology Facility > > Stowers Institute for Medical Research 1000 E. 50th St. > > Kansas City, MO 64110 > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Anne van Binsbergen (Hope) > Abu Dhabi > UAE > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From jennifer.l.hofecker <@t> Vanderbilt.Edu Wed May 14 06:47:25 2008 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Wed May 14 06:47:31 2008 Subject: [Histonet] Sakura VIP flood? In-Reply-To: <1CE1847DFEA0A647B1CCDE4108EA60A747A34C@LTA3VS011.ees.hhs.gov> References: <004801c8b5b1$d08ab810$0302a8c0@yourxhtr8hvc4p> <1CE1847DFEA0A647B1CCDE4108EA60A747A34C@LTA3VS011.ees.hhs.gov> Message-ID: <898D946569A27444B65667A49C0740520175B454@mailbe06.mc.vanderbilt.edu> Hi histo netters! I just wanted to let you know that Teri is not the only one to experience this. In a previous lab, we had Niagara falls coming out of our VIP. I can't remember what was diagnosed (6-ish years ago) but the Sakura guy was able to fix it. I do remember it was a part inside the machine, not how full our water container was. I tell people all the time about that morning. You haven't seen anything until your processor is spewing water... At least it was water. Good Luck! Jennifer L. Hofecker HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph 615.343.0083 fax 615.343.7089 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Wednesday, May 14, 2008 6:36 AM To: Anne van Binsbergen; Joe Nocito Cc: histonet@lists.utsouthwestern.edu; Johnson,Teri Subject: RE: [Histonet] Sakura VIP flood? If it was an issue of overfilling like Sakura said then more people would have experienced it because I am sure most of us fill to between 2-3 liters. I think it is something else, definitely! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne van Binsbergen Sent: Wednesday, May 14, 2008 7:11 AM To: Joe Nocito Cc: histonet@lists.utsouthwestern.edu; Johnson, Teri Subject: Re: [Histonet] Sakura VIP flood? WOW!! am very curious to know what Sakura tells you also have a 'trusty VIP5 workhorse' in my stable - but never one that has flooded!! keep us in the loop Annie 2008/5/14 Joe Nocito : > Gee Teri, > that's a first for me. I've used the VIP 5 for years and never had > this happen. I know, I've been a lot of help. > > JTT > ----- Original Message ----- From: "Johnson, Teri" > > To: > Sent: Tuesday, May 13, 2008 12:46 PM > Subject: [Histonet] Sakura VIP flood? > > > Any of you users of the Sakura VIP 5 have any problems with the fume > control water somehow siphoning and flooding into the guts of the > instrument? The manual tells us to put between 2-3 liters of water in there. > We had issues with this happening early on, and were told we had too > much water in there. We now use much less, and until 3 days ago had no > problem with it. This morning came in to water all in the overflow > tray, dripping down from the inside of the instrument on to the floor, > and an empty fume control water bottle. > > My in house service guy will be calling Sakura today, but wanted to > know of others' experiences with this issue. I'm not interested in > bashing the product, it's worked well for us for over 6 years. Just > wondering if it's happened to anybody else. > > Thanks! > > Teri Johnson, HT(ASCP)QIHC > Managing Director Histology Facility > Stowers Institute for Medical Research 1000 E. 50th St. > Kansas City, MO 64110 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From paintedsplashes <@t> yahoo.com Wed May 14 07:24:58 2008 From: paintedsplashes <@t> yahoo.com (Jeanne Clark) Date: Wed May 14 07:25:07 2008 Subject: [Histonet] IHC protocols Message-ID: <862071.89097.qm@web30706.mail.mud.yahoo.com> I am curious how labs determine the drying time and temps on their IHC slides prior to retrieval and IHC staining? Overnight at RT, 1 hour at 60 C, 30 min at 70 C? I have seen all these in use but no actual documentation of why? Jeanne Clark [40.gif] 233 McCarty Hollow Road Telford, Tn. 37690 423-612-1213 From relia1 <@t> earthlink.net Wed May 14 07:36:42 2008 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed May 14 07:36:54 2008 Subject: [Histonet] RELIA Histology Job Alert 5/14/08 Message-ID: Hi Histonetters! I just want to take a minute to tell you about my most exciting current opportunities. All of these positions are full time permanent dayshift positions. My clients are the premier facilities in their areas and offer excellent compensation commisurate with your experience and the cost of living in the area. In addition they offer excellent benefits and relocation assistance. If you or anyone you know might be interested in hearing more about these opportunities please let me know. You can respond to this e-mail relia1@earthlink.net or call me toll free at 866-607-3542. Here are the jobs I want to tell you about: Special Stains Tech - San Antonio Immunohistochemistry Tech - San Antonio P/A-Grossing Histotech - Frederick, MD Histotech - Frederick, MD Histotech - Atlanta, GA Histotechnologist - NYC Histotechs - several positions throughout the Tampa Bay Area New Grads and Experienced Techs are welcome to apply. Remember it never hurts to look! Thanks-Pam 866-607-3542 relia1@earthlink.net Have a Great Day!! Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.myspace.com/pamatrelia From kmerriam2003 <@t> yahoo.com Wed May 14 07:59:19 2008 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Wed May 14 07:59:30 2008 Subject: [Histonet] slide scanner Message-ID: <459784.90885.qm@web50308.mail.re2.yahoo.com> I have had really good luck with the Aperio slide scanner, but it is not cheap! ?Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: "Stephens, Elizabeth Humes" To: histonet@lists.utsouthwestern.edu Sent: Tuesday, May 13, 2008 3:13:00 PM Subject: [Histonet] slide scanner Does anybody have a recommendation for an inexpensive but reliable histology slide scanner? We have the Microtek Artixscan 4000tf and use the Microtek ScanWizard Pro TX software. The machine works fine but the software keeps crashing. We downloaded the most recent version (Scanwizard Pro TX7) but still am having problems on both Mac and PC, lab and personal computers. Thanks for your advice!! Elizabeth Stephens _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed May 14 08:08:01 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 14 08:08:11 2008 Subject: [Histonet] salary scales In-Reply-To: <0E8C20BD05BC44B9BE7B2C9E16CF4FC6@JimPC> Message-ID: <293569.75122.qm@web65703.mail.ac4.yahoo.com> Jim: All those "collateral" payments you mention, as true as they are, are YOUR cost for doing business and they require that YOU try to exchange all of them for a high productivity level in order to "receive" a certain work amount for the salary component of your costs (that is, at least 70% of your production costs). All those things are true and become the incentives for some employees to work on some companies and avoid others. Having said and acknowledging all that, they have NOTHING to do with the NET payment your employees receive and that have to be used to pay for rent/mortgage, food, gasoline and all the other things that have to come out of the NET "leftover" of their GROSS salary (and remember that the $25/hour you mention is GROSS salary) so, yes additional benefits are important, but the net amount received as work payment are more "meaningful" from the practical point of view. Ren? J. jstaruk wrote: From b-frederick <@t> northwestern.edu Wed May 14 08:14:10 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed May 14 08:14:28 2008 Subject: [Histonet] salary scales In-Reply-To: Message-ID: <000001c8b5c4$689c7280$d00f7ca5@lurie.northwestern.edu> Fine look at the benefits and then tell me that if the cost of the health insurance goes up 20% (causing one to have to go to a higher deductible), my train fare goes up 10%, gas is high and though I am single I own a home and therefore am responsible for all those associated costs. Now how does a raise cover all that? That's why we gripe about what is on the pay scale. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Showers, Doug Sent: Tuesday, May 13, 2008 5:58 PM To: jstaruk; Rene J Buesa; Charles.Embrey; godsgalnow@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] salary scales I agree that the prospective employee needs to weigh all of the other benefits, but frankly most do not. The cost of benefits to the employer is usually of little or no interest to the employee. Most simply want to know how much they will be taking home after taxes, insurance, etc. I have interviewed a lot of job applicants over the years and most of them give the benefits a cursory glance. I do think that if you offer great benefits your current employees spread the good word and attract more applicants for your positions. Often, during the years I spent in the Air Force, I would get a mailing from the military laying out the dollar cost of all of my benefits and I remember thinking "If I could get a little more of that in my paycheck I might be a happier person." Doug Showers MS, HT (ASCP) ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of jstaruk Sent: Tue 5/13/2008 5:46 PM To: 'Rene J Buesa'; 'Charles.Embrey'; godsgalnow@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] salary scales As president and owner of a histology lab (although this can be any business), I feel I must add my thoughts to this discussion on salary (which pops up on this newsgroup quite often). First of all, there's more to a "salary" that what the number is on your weekly pay stub. Who's paying for your health insurance? Does your company offer a matching 401K plan? Life insurance? How many weeks paid vacation do you get? How many paid holidays? Paid sick days? Can you leave when your job is complete (and still get paid for the rest of the day)? Does your company pay for your lunch hour? Do they pay for your lunch? Do they offer child care? A work-out room? There's a heck of a lot more many companies do for their employees than simply paying them a certain rate for every hour they are "punched in". I figure any employee who works at my company for "only" $25.00 per hour is actually making about $55.00 per hour if they utilize all (or at least most) of the benefits we offer them. Then there's the matching social security, worker's Comp. insurance, state employee taxes and so on that we're also responsible to pay for each employee. Think of all this next time you say you only make $25.00 per hour! Jim _____________________ Jim Staruk Mass Histology Service www.masshistology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, May 13, 2008 5:17 PM To: Charles.Embrey; godsgalnow@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] salary scales Charles: No, I don't think it is "a rather broad statement". Regardless of the area of the country, you should never negotiate a salary as part of a group. You are "selling" your personal abilities and trying to cover your family needs. How different is the gasoline price today by regions? Are they so different? How about food products?. I encourage you to read my article in Advance for MLP (Jan.28, 2008) where I demonstrate that all the "alleged" salary regional variations are non statistically significant and offer some advise in how to negotiate your salary. Ren? J. "Charles.Embrey" wrote: _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed May 14 08:14:48 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 14 08:14:51 2008 Subject: [Histonet] Standardized Microtomes In-Reply-To: <582736990805131710s398184e7oaa2d5d3ae40e4b43@mail.gmail.com> Message-ID: <211778.92149.qm@web65707.mail.ac4.yahoo.com> All in all the practice of "changing the angle to match the block you have to recut as needed" is not a good practice. During it you could end loosing tissue when trying to "build" a new flat surface from a block that was originally cut at a different angle, while "sculping" the new surface. The best practice is to have all the microtomes cutting at the same angle and try to convince those HTs that say that "I have always cut with this angle" that they are flat wrong and a general angle can be good for the majority of blocks. Ren? J. Amos Brooks wrote: From Charles.Embrey <@t> carle.com Wed May 14 08:19:31 2008 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Wed May 14 08:19:36 2008 Subject: [Histonet] salary scales In-Reply-To: <405971.85367.qm@web65709.mail.ac4.yahoo.com> References: <44780C571F28624DBB446DE55C4D733A1FE5BA@EXCHANGEBE1.carle.com> <405971.85367.qm@web65709.mail.ac4.yahoo.com> Message-ID: <44780C571F28624DBB446DE55C4D733A1FE5BC@EXCHANGEBE1.carle.com> Rene, I agree with you. I think salaries across the board for histotechs are below what they should be but Roxanne asked the statement from an employer's perspective and not an employee. She wanted to know if her range was completive and depending on the part of the country, (Yes, it can make a difference in home prices and in being where people want to live and work. The "Sunshine Tax" is a great example), the range she stated would be. If she were in a lab just down the road from me my histotechs would swarm from my lab to apply at her lab. As an employer, you look to protect the bottom line and offer the lowest salary you can and still be attractive to potential employees. It will only change when the lower paying labs can't find help and salaries will rise. I am watching that happen even now as this country faces a technician shortage. It all boils down to supply and demand. Chuck ________________________________ From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Tuesday, May 13, 2008 4:35 PM To: Charles.Embrey Subject: RE: [Histonet] salary scales Charles: Attached 2 articles I wrote on the salary subject that explain my personal position on this issue. As long as HTs keep accepting misery salaries (always BELOW those of the MTs) we will keep being considered as a "second class technical personnel". Ren? J. "Charles.Embrey" wrote: From b-frederick <@t> northwestern.edu Wed May 14 08:23:07 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed May 14 08:23:20 2008 Subject: [Histonet] About phosphate buffer - simple (silly?) question In-Reply-To: <8c964a790805132340j74003539jff138257f7d7897d@mail.gmail.com> Message-ID: <000101c8b5c5$a8845a10$d00f7ca5@lurie.northwestern.edu> But it premade from Fisher or just about anyone else.. 10% NBF. Why bother with the math? You can get it in a 5 gallon container. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jorge Tornero Sent: Wednesday, May 14, 2008 1:41 AM To: histonet Subject: Re: [Histonet] About phosphate buffer - simple (silly?) question Hi Ann and All, I know about those recipes, but I would rather prefer to be able to calculate it by myself and solve my problem "for ever" Thank you very much anyway Jorge Tornero IEO-C?diz Spain 2008/5/14 Anne van Binsbergen : > Hi Jorge > google 'neutral buffered formalin' and you will have the recipe for NBF > Anne > > 2008/5/14 Jorge Tornero : > > > Hi, > > > > I have to prepair phosphate buffered formaline to preserve anchovy > > gonads. I > > am pretty new in this bussines, and unfortunately my knowledge on > > chemistry > > is far from ideal. > > > > When I started my work some time ago, I "inherited" some formulas about > > the > > matter, but soon I've realized that those formulas are imprecise and > > vague: > > for instance, they just say "mix x grams of dibasic salt with y grams of > > monobasic salt", and those x's and y's vary from one formula to other. > > Of > > course, I can't tell how many hydration water molecules are considered > > for > > the chemical species in the formulas, and of course there is nothing > > about > > the purity of the salts used. So I've decided to try to make the > > calculations by myself, thus solving the problem for every time I need > > to > > make this buffer or other. > > > > So I have been looking around and found the Henderson-Hasselbalch > > equation > > and I've employed it to make calculations, and everything is ok, BUT... > > > > The dissociation constant for phosphate, pKa, seems to be in most cases > > 7.2, > > but I've found, in several places, to be 6.86. They say it is a apparent > > pKa > > (they call it pKa') due to the ionic strenght of the solution. The > > problem > > is: Which pKa should I use? I guess the correct is 6.86, but I don't > > know > > the reasons for it. I've read that pKa'=pKa+correction factor, > > calculations > > involving ionic strenght but I am not able to find the tables where the > > correction factor for a given ionic strenght are tabulated. > > > > So my quesion is: Which pKa should I use? Why? How to calculate that > > pKa? > > > > I hope my bad english is not a problem for you to understand me. > > > > Best Regards, > > > > Jorge Tornero > > IEO-C?diz > > Spain > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Anne van Binsbergen (Hope) > Abu Dhabi > UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed May 14 08:25:34 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 14 08:32:23 2008 Subject: [Histonet] About phosphate buffer - simple (silly?) question In-Reply-To: <8c964a790805132340j74003539jff138257f7d7897d@mail.gmail.com> Message-ID: <738078.64164.qm@web65709.mail.ac4.yahoo.com> Then you should get a good chemistry book and learn. Ann gave you a good advise but if you don't want to take, you will have to learn chemistry. Ren? J. Jorge Tornero wrote: Hi Ann and All, I know about those recipes, but I would rather prefer to be able to calculate it by myself and solve my problem "for ever" Thank you very much anyway Jorge Tornero IEO-C?diz Spain 2008/5/14 Anne van Binsbergen : > Hi Jorge > google 'neutral buffered formalin' and you will have the recipe for NBF > Anne > > 2008/5/14 Jorge Tornero : > > > Hi, > > > > I have to prepair phosphate buffered formaline to preserve anchovy > > gonads. I > > am pretty new in this bussines, and unfortunately my knowledge on > > chemistry > > is far from ideal. > > > > When I started my work some time ago, I "inherited" some formulas about > > the > > matter, but soon I've realized that those formulas are imprecise and > > vague: > > for instance, they just say "mix x grams of dibasic salt with y grams of > > monobasic salt", and those x's and y's vary from one formula to other. > > Of > > course, I can't tell how many hydration water molecules are considered > > for > > the chemical species in the formulas, and of course there is nothing > > about > > the purity of the salts used. So I've decided to try to make the > > calculations by myself, thus solving the problem for every time I need > > to > > make this buffer or other. > > > > So I have been looking around and found the Henderson-Hasselbalch > > equation > > and I've employed it to make calculations, and everything is ok, BUT... > > > > The dissociation constant for phosphate, pKa, seems to be in most cases > > 7.2, > > but I've found, in several places, to be 6.86. They say it is a apparent > > pKa > > (they call it pKa') due to the ionic strenght of the solution. The > > problem > > is: Which pKa should I use? I guess the correct is 6.86, but I don't > > know > > the reasons for it. I've read that pKa'=pKa+correction factor, > > calculations > > involving ionic strenght but I am not able to find the tables where the > > correction factor for a given ionic strenght are tabulated. > > > > So my quesion is: Which pKa should I use? Why? How to calculate that > > pKa? > > > > I hope my bad english is not a problem for you to understand me. > > > > Best Regards, > > > > Jorge Tornero > > IEO-C?diz > > Spain > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Anne van Binsbergen (Hope) > Abu Dhabi > UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgalnow <@t> aol.com Wed May 14 08:38:34 2008 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Wed May 14 08:39:02 2008 Subject: [Histonet] salary scales In-Reply-To: <44780C571F28624DBB446DE55C4D733A1FE5BC@EXCHANGEBE1.carle.com> References: <44780C571F28624DBB446DE55C4D733A1FE5BA@EXCHANGEBE1.carle.com> <405971.85367.qm@web65709.mail.ac4.yahoo.com> <44780C571F28624DBB446DE55C4D733A1FE5BC@EXCHANGEBE1.carle.com> Message-ID: <8CA83DEF100ED11-B18-132@FWM-D28.sysops.aol.com> To all that are curious...I am in the Tampa Bay area in Florida and we are within a stones throw to Quest, Ameripath and LabCorp...we are trying to compete so we can keep the employees we have and to make sure they are paid appropriately. While I am looking at this from an employer's perspective, I am also one of the employees, so it does benefit me as well. Roxanne -----Original Message----- From: Charles.Embrey To: Rene J Buesa Cc: histonet@lists.utsouthwestern.edu Sent: Wed, 14 May 2008 9:19 am Subject: RE: [Histonet] salary scales Rene, I agree with you. I think salaries across the board for histotechs are elow what they should be but Roxanne asked the statement from an employer's erspective and not an employee. She wanted to know if her range was completive nd depending on the part of the country, (Yes, it can make a difference in home rices and in being where people want to live and work. The "Sunshine Tax" is a reat example), the range she stated would be. If she were in a lab just down he road from me my histotechs would swarm from my lab to apply at her lab. As n employer, you look to protect the bottom line and offer the lowest salary you an and still be attractive to potential employees. It will only change when he lower paying labs can't find help and salaries will rise. I am watching hat happen even now as this country faces a technician shortage. It all boils own to supply and demand. Chuck ________________________________ From: Rene J Buesa [mailto:rjbuesa@yahoo.com] ent: Tuesday, May 13, 2008 4:35 PM o: Charles.Embrey ubject: RE: [Histonet] salary scales Charles: Attached 2 articles I wrote on the salary subject that explain my personal osition on this issue. As long as HTs keep accepting misery salaries (always ELOW those of the MTs) we will keep being considered as a "second class echnical personnel". Ren? J. "Charles.Embrey" wrote: _______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From ryaskovich <@t> dir.nidcr.nih.gov Wed May 14 08:56:03 2008 From: ryaskovich <@t> dir.nidcr.nih.gov (Yaskovich, Ruth A (NIH/NIDCR) [E]) Date: Wed May 14 08:57:37 2008 Subject: [Histonet] slide scanner In-Reply-To: <459784.90885.qm@web50308.mail.re2.yahoo.com> References: <459784.90885.qm@web50308.mail.re2.yahoo.com> Message-ID: I second that Kim! It's wonderful! Ruth Yaskovich National Institutes of Health National Institute of Dental and Crainiofacial Research Neurobiology and Pain Therapeutics -----Original Message----- From: Kim Merriam [mailto:kmerriam2003@yahoo.com] Sent: Wednesday, May 14, 2008 8:59 AM To: Stephens, Elizabeth Humes; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] slide scanner I have had really good luck with the Aperio slide scanner, but it is not cheap! ?Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: "Stephens, Elizabeth Humes" To: histonet@lists.utsouthwestern.edu Sent: Tuesday, May 13, 2008 3:13:00 PM Subject: [Histonet] slide scanner Does anybody have a recommendation for an inexpensive but reliable histology slide scanner? We have the Microtek Artixscan 4000tf and use the Microtek ScanWizard Pro TX software. The machine works fine but the software keeps crashing. We downloaded the most recent version (Scanwizard Pro TX7) but still am having problems on both Mac and PC, lab and personal computers. Thanks for your advice!! Elizabeth Stephens _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed May 14 08:33:07 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 14 08:59:55 2008 Subject: [Histonet] Standardized Microtomes In-Reply-To: <898D946569A27444B65667A49C0740520175B453@mailbe06.mc.vanderbilt.edu> Message-ID: <38930.74828.qm@web65711.mail.ac4.yahoo.com> They are not manufactured with a "standard angle" because according with the RESISTANCE of the material to cut, due to the intrinsic characteristics of the tissue (more or less "solid" or "resistant") you should experiment with the "ideal angle" for that particular block. In the general pathology practice being all human tissues and most of them infiltrated with the same melting point paraffin, they "become" of a "quasi standard" resistance level that can allow a single angle for all microtomes. Under those circumstances it is better to have all microtomes working at the same angle and allowing any HT to recut a block initially cut by any one of the other HTs in the lab. Ren? j. "Hofecker, Jennifer L" wrote: From rjbuesa <@t> yahoo.com Wed May 14 08:38:19 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 14 09:05:07 2008 Subject: [Histonet] IHC protocols In-Reply-To: <862071.89097.qm@web30706.mail.mud.yahoo.com> Message-ID: <273498.62853.qm@web65702.mail.ac4.yahoo.com> This aspect, and many others as well, are the result of the lack of standardization in most of our procedures (that are not staining procedures with specific times per step). Each lab pretty much accomplish its goals by doing things "their way" usually the "way of the master/Senior HT" that "set in stone" the general procedures (until s/he retires and a new senior HT arrives with "new and better" ways of doing things). Ren? J. Jeanne Clark wrote: From jennifer.harvey <@t> Vanderbilt.Edu Wed May 14 09:12:21 2008 From: jennifer.harvey <@t> Vanderbilt.Edu (Harvey, Jennifer Lynn) Date: Wed May 14 09:12:30 2008 Subject: [Histonet] Standardized Microtomes In-Reply-To: <898D946569A27444B65667A49C0740520175B453@mailbe06.mc.vanderbilt.edu> References: <582736990805131710s398184e7oaa2d5d3ae40e4b43@mail.gmail.com> <898D946569A27444B65667A49C0740520175B453@mailbe06.mc.vanderbilt.edu> Message-ID: I totally agree. I am "pro-angling"!!! If you don't angle then how do you get a full cut? I haven't had to many pieces of tissue totally flat an a little angling is sometimes needed gets a full cut. I can't imagine not angling to get the best representative section. I am taking the flames with you! Jennifer Harvey, HT(ASCP) QIHC Vanderbilt Vision Research Center RM 8105 MCE North Tower 1215 21st Ave. South Nashville, TN 37232-8808 Phone: 615-936-1486 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hofecker, Jennifer L Sent: Wednesday, May 14, 2008 6:45 AM To: Amos Brooks Cc: histonet Subject: RE: [Histonet] Standardized Microtomes Ok, I have donned my flame resistant suit... I agree 100% with Amos! I learned to angle the block when I learned to cut and I have worked in a few labs where that was "forbidden". It always seemed so silly to me that instead of taking 0.5 seconds to angle the block and get a perfect full face of the tissue that I had to spend 15 minutes re-embedding the block. I actually had to tape the adjustment knobs on my microtome so I wouldn't "break the rules". One might think that if you angle the block, how will the next tech be able to "line it up"? Answer: If everyone knows how to angle the block, it's not an issue. One other question: If we are never supposed to angle the block, why are those little knobs on the block holder? Why don't microtome manufacturers make them all "standard" and not adjustable? I choose to believe that we are highly skilled individuals and that patient care is best served by us producing quality work. I am "pro-angling" all the way! I know... I know... Have a great day everyone! Jennifer L. Hofecker HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph 615.343.0083 fax 615.343.7089 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Tuesday, May 13, 2008 7:11 PM To: nancy_schmitt@pa-ucl.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] Standardized Microtomes Hi Nancy, We do not do this, because one of the first things one should learn when learning how to cut is how to change the angle of whatever microtome you are cutting at. Setting them all to the same angle just makes learning how to use those funny little knobs on the top of the block holder pointless. So when a situation arises theat requires you to change the angle no one knows how to do it. The solution, just cut into it and the block will be the same angle, or re-embed the tissue (astonishingly lazy- extra work to accomplish nothing) and trim it back to the angle that is on all the microtomes thereby loosing tissue anyway! A far better solution, teach techs to adjust the block holder without loosing tissue (advance slowly)! Give a man a fish & he'll eat for a day. Teach a man to fish and he'll never go hungry. Just my $0.02 Amos Brooks Message: 17 Date: Tue, 13 May 2008 11:28:24 -0500 From: Nancy Schmitt Subject: [Histonet] Standardized Microtomes To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <9FC023A4AB52BB4D87DC6456081A822C087C8D@mercury.pa-ucl.com> Content-Type: text/plain; charset="iso-8859-1" Good Morning to all Does anybody standardize microtomes so they all cut at same angle? Is this impossible? We only cut control blocks on one microtome because they are each at a little different angle. Would be nice to be able to do at all........... Thanks for any feedback Nancy Schmitt Histology Coordinator Dubuque, IA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JSCHUMA1 <@t> Fairview.org Wed May 14 09:51:16 2008 From: JSCHUMA1 <@t> Fairview.org (Schumacher, Jennifer J) Date: Wed May 14 10:26:34 2008 Subject: [Histonet] DRS 60 staining dishes Message-ID: A few weeks ago, I requested staining dishes from a colleague on histonet. However, I mis-read the email. I have a DRS 601 stainer, and the dishes are for the DRS 60 stainer. If anyone else could use these twelve staining dishes, I would be happy to send them to you. Send me your fedex number and address and I'll ship them out to anyone who wants them. Thank you. Jennifer Jennifer Schumacher CLS Lead, Pathology Riverside Campus University of Minnesota Medical Center, Fairview 2450 Riverside Ave Minneapolis, MN 55454 Phone 612.273.2883 Pager 612.899.6038 Fax 612.273.9124 email jschuma1@fairview.org From yourbiomed <@t> cox.net Wed May 14 10:52:09 2008 From: yourbiomed <@t> cox.net (YourBiomed.Com ) Date: Wed May 14 10:52:14 2008 Subject: [Histonet] VIP Flooding Message-ID: <20080514115209.XD95J.15315.imail@fed1rmwml34> All, More than likely the rotary valve is to blame. The rotary valve ?When not working correctly? can pickup from one station and dump into another. This can happen if the rotary valve o-rings or sealing internal is not to manufacturer specs. It can also happen if after it picked fluid from one station and then the rotary valve advanced a few steps and returned fluid into incorrect station "Bottle" It is true this particular processor is a "Work Horse" I'm betting you had one bottle low on fluid or empty and another full? Usually the bottles ?stations? are next to each other. Anyway this can be caused by many issues, here are a few. 1. The position sensor on the rotary valve is faulty. 2. The rotary valve seals are compromised. 3. A protocol can become corrupt. From gu.lang <@t> gmx.at Wed May 14 09:22:17 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed May 14 11:09:04 2008 Subject: AW: [Histonet] Help! In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A382E@IS-E2K3.grhs.net> Message-ID: <81E2E82A7FCD4A54A4565AF6B90679A1@dielangs.at> Nordiqc says: Appendix can be used as positive control: The nerves must be as strongly stained as possible, while no staining of the epithelial and smooth muscle cells shall be seen. Further information on http://www.nordiqc.org/Run-19/assessment/assessment-CR.htm Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Mike Pence Gesendet: Dienstag, 13. Mai 2008 19:59 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Help! I am in need of a calretinin control block. Can anyone tell me of anything other than lung mesothelioma tissue that will work in a pinch or if anyone would have a block you would be willing to send me? I will cover the shipping. Thanks, Mike Pence Great River Medical Center Dept. of Pathology West Burlington, IA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dshowers <@t> dermpathdiagnostics.com Wed May 14 11:12:21 2008 From: dshowers <@t> dermpathdiagnostics.com (Showers, Doug) Date: Wed May 14 11:12:31 2008 Subject: [Histonet] Standardized Microtomes References: <38930.74828.qm@web65711.mail.ac4.yahoo.com> Message-ID: They are not manufactured with a fixed angle for several reasons. The primary reason is that due to inconsistencies in embedding the plastic cassette on the back of the block, the face of the block is just not always going to be flush with the edge of the knife. A secondary reason is to change the angle of the block face to match the knife edge when doing recuts, etc. Back in the stone age with hinged, L-shaped, and the grid molds the back of the block was never flat so techs became adept at "eyeballing" the angle when the block was put in the microtome clamp. Fine tuning was then done using the adjustment screws. With the introduction of embedding rings and base molds, there was less need for such adjusting and even less need with our present day processing/embedding cassettes and base molds. So I am not surprised that a lot of places have techs who do not ever change their angle. I agree that it is a necessary skill that should be taught. I also think that adjusting the knife holder back to a neutral position in relation to the knife edge should become part of each microtomes' care, maybe on a weekly basis. Doug Showers MS, HT (ASCP) Operations Manager Dermpath Diagnostics VM 561-712-6283 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Wed 5/14/2008 8:33 AM To: Hofecker, Jennifer L; Amos Brooks Cc: histonet Subject: RE: [Histonet] Standardized Microtomes They are not manufactured with a "standard angle" because according with the RESISTANCE of the material to cut, due to the intrinsic characteristics of the tissue (more or less "solid" or "resistant") you should experiment with the "ideal angle" for that particular block. In the general pathology practice being all human tissues and most of them infiltrated with the same melting point paraffin, they "become" of a "quasi standard" resistance level that can allow a single angle for all microtomes. Under those circumstances it is better to have all microtomes working at the same angle and allowing any HT to recut a block initially cut by any one of the other HTs in the lab. Ren? j. "Hofecker, Jennifer L" wrote: _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rosenfeldtek <@t> hotmail.com Wed May 14 11:28:50 2008 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Wed May 14 11:28:55 2008 Subject: [Histonet] Salary Scales Message-ID: Last year I hired a Histologist and started him at $54,500 a year. It seemed a little steep at the time, but it really improved our research productivity and helped me sleep at night to have a organized person who really, really knew their stuff focussing on that bit of the work--so I didn't have to. I've decided it is unreasonable to expect to be able to do science on the cheap, and my strategy now for recruting and retaining the best workers is--pay them! Jerry Ricks Research Scientist University of Washington Department of Pathology _________________________________________________________________ Make Windows Vista more reliable and secure with Windows Vista Service Pack 1. http://www.windowsvista.com/SP1?WT.mc_id=hotmailvistasp1banner From dcrippen <@t> buckinstitute.org Wed May 14 11:31:36 2008 From: dcrippen <@t> buckinstitute.org (Danielle Crippen) Date: Wed May 14 11:31:48 2008 Subject: [Histonet] unsubscribe Message-ID: Danielle Crippen Morphology and Imaging Core x2046 From akemiat3377 <@t> yahoo.com Wed May 14 11:34:30 2008 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Wed May 14 11:34:35 2008 Subject: [Histonet] e-mails forwarded to Chuck Churukian Message-ID: <801611.86084.qm@web31305.mail.mud.yahoo.com> Hi All, To those of you that sent e-mails to Chuck for his 80th birthday, I just wanted to let you know that I forwarded all of them to Chuck. I called him yesterday to give him a heads-up. I wanted to make sure he didn't start deleating all of them. He was very touched and appreciative. Chuck wanted me to extend his appreciation. Thanks, Akemi Akemi Allison-Tacha, BS, HT(ASCP)HTL Client Services Manager PhenoPath laboratories 551 North 34th Street, Suite 100 Seattle, WA 98103-8675 Work: (206) 374-9000 ext 1053 E-Mail: akemiat3377@yahoo.com From POWELL_SA <@t> Mercer.edu Wed May 14 10:07:06 2008 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Wed May 14 11:38:51 2008 Subject: [Histonet] FW: REMINDER! Invitation to Symposia: Advances in Histology and Immunohistochemistry Techniques Message-ID: <01MURYX5J2MC004UVO@Macon2.Mercer.edu> FYI, Here is the program for the FREE IHC workshop in Atlanta at Emory next Wednesday, May 21. Subject: REMINDER! Invitation to Symposia: Advances in Histology and Immunohistochemistry Techniques The 2008 Symposia Series The Biosystems Division of Leica Microsystems cordially invites you to attend the 2008 Symposia Series "Advances in Histology and Immunohistochemistry Techniques" You will learn about exciting industry advances that are shaping the histology laboratory, earn free CEU credits, and see demonstrations of state-of-the-art laboratory solutions for your workflow challenges. Keynote Speaker: Dr. Cynthia Cohen, Deprtment of Pathology, Emory University Hospital, "In Situ Hybridization in Today's ImmunoPathology Laboratory" Guest Speakers: Jim Burchette, HT (ASCP) QIHC, Director of ImmunoPathology, Duke University, "Specialized Immunohistochemistry and Troubleshooting Techniques" Dr. Mark Rees, Molecular Biology Manager, Novocastra, Leica Microsystems, "The First Quantitative Comparison of Immunohistochemical Rabbit and Mouse Monoclonal Antibody Affinities Using Biacore Analysis" David White, Group Marketing Manager, Leica Microsystems, "Histology: Maximizing Quality and Workflow" Date: Wednesday, May 21, 2008, Time: 9:00 to 3:30 p.m. (8:30 registration) Place: Emory Conference Center, 1615 Clifton Road, Atlanta, GA At noon we invite all attendees to enjoy a complimentary lunch and the chance to network. Please R.S.V.P. to help us plan the event. Email broadspectrumhistology@leica-microsystems.com Leica Microsystems plans to sponsor future symposia in California, Boston, and Washington D.C. Please contact your local Leica Microsystems representative to learn more! You are subscribed as: powell_sa@mercer.edu. To unsubscribe this email address, please click here From tammy <@t> surgicalpathlabs.com Wed May 14 11:51:58 2008 From: tammy <@t> surgicalpathlabs.com (Tammy de Leon) Date: Wed May 14 11:52:03 2008 Subject: [Histonet] HT/HTL Positions Available in Florida In-Reply-To: <01MURYX5J2MC004UVO@Macon2.Mercer.edu> Message-ID: SPL, a CAP accredited Pathology Laboratory in Pinellas Park is currently seeking Histotechnologists, HT/HTL ASCP Preferred. Primary responsibilities include on-site frozen sections including working in our mobile lab units. We offer competitive pay, a comprehensive benefits package (100% of medical, dental, LTD, & life premiums are paid by SPL!!) We offer flexible schedule opportunities!! 5 day work weeks or 4 ten hour days allowing for LONG WEEKENDS and more family time!!!!! Please forward resume to tammy@surgicalpathlabs.com , call 727-545-2339 or fax 727-545-1644. Tammy R. de Leon Surgical Pathology Laboratories Human Resources Officer 7641 66th Street N Suite C Pinellas Park, Fl 33781 Office Phone - 727-545-2339 Fax - 727-545-1644 From mlgiebel <@t> vcu.edu Wed May 14 11:56:51 2008 From: mlgiebel <@t> vcu.edu (Mary L Giebel/FS/VCU) Date: Wed May 14 11:56:57 2008 Subject: [Histonet] ZYMED Histostain Plus Kit on animal tissue Message-ID: At a client's request, I am using a ZYMED's Broad Spectrum Histostain Plus Kit (#85-9843) on rat tissue. I have encountered an unexpected staining pattern as well as more background staining than usual. I would be very interested in hearing from others who have used this kit on animal tissue. I found the question posed in the Histonet archives (8/23/2005), but never found an answer posted. Thank you for your help. Regards, Mary Giebel From Ngale <@t> bccancer.bc.ca Wed May 14 12:05:39 2008 From: Ngale <@t> bccancer.bc.ca (Gale, Nadia) Date: Wed May 14 12:05:53 2008 Subject: [Histonet] Glucose oxidase method for blocking endogenous peroxidase Message-ID: I've searched the archives and cannot find what I am after... I'd like to try the glucose oxidase method for blocking endogenous peroxidase in tissue sections for IHC. What is a suitable replacement for the B-d(+)glucose G5250 discontinued by Sigma? Will the D-(+)-Glucose G8270 work? This is 4% beta isomer and 96% alpha. Thanks for your help, Nadia Nadia Gale Lead Histotechnologist Centre for Translational and Applied Genomics (CTAG) 3427-600 West 10th Avenue Vancouver, BC V5Z 4E6 tel 604-877-6000 ext 2426 fax 604-877-6089 ngale@bccancer.bc.ca From Pam.Bakken <@t> childrensmn.org Wed May 14 12:39:57 2008 From: Pam.Bakken <@t> childrensmn.org (Pam Bakken) Date: Wed May 14 12:40:22 2008 Subject: [Histonet] Cutting research blocks from home? Message-ID: <482ADD9D.37F9.0000.0@childrensmn.org> Hi, I am interested in working part time from home cutting research blocks. Is there anyone that could give me some advice on how to get started? How do I charge? Any experience, advice or knowledge would be greatly appreciated. Thanks, Pamm Bakken Children's Hospital - Minneapolis _______________________________________________________________ Confidentiality Statement: This email/fax, including attachments, may include confidential and/or proprietary information and may be used only by the person or entity to which it is addressed. If the reader of this email/fax is not the intended recipient or his or her agent, the reader is hereby notified that any dissemination, distribution or copying of this email/fax is prohibited. If you have received this email/fax in error, please notify the sender by replying to this message and deleting this email or destroying this facsimile immediately. From kmerriam2003 <@t> yahoo.com Wed May 14 12:51:11 2008 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Wed May 14 12:51:18 2008 Subject: [Histonet] ZYMED Histostain Plus Kit on animal tissue Message-ID: <654253.27119.qm@web50308.mail.re2.yahoo.com> I dont have experience with this particular kit from Zymed, but the term "broad spectrum" makes me think that it?would be similar to a universal secondary (that might include anti-mouse and anti-rabbit secondaries) which would likely cross-react to your rat tissue.? It might be worth giving Zymed a call to find out about this kit. ?Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: Mary L Giebel/FS/VCU To: histonet@lists.utsouthwestern.edu Sent: Wednesday, May 14, 2008 12:56:51 PM Subject: [Histonet] ZYMED Histostain Plus Kit on animal tissue At a client's request, I am using a ZYMED's Broad Spectrum Histostain Plus Kit (#85-9843) on rat tissue. I have encountered an unexpected staining pattern as well as more background staining than usual. I would be very interested in hearing from others who have used this kit on animal tissue. I found the question posed in the Histonet archives (8/23/2005), but never found an answer posted. Thank you for your help. Regards, Mary Giebel ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed May 14 14:18:02 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 14 14:18:06 2008 Subject: [Histonet] Cutting research blocks from home? In-Reply-To: <482ADD9D.37F9.0000.0@childrensmn.org> Message-ID: <734.60655.qm@web65716.mail.ac4.yahoo.com> After getting the "essentials" (microtome, water bath, oven, staining dishes and needed supplies) you have to decide a price. Charge by the slide. One H&E stain costs about $4.50, with everything else (from cutting to staining) I would charge $10/stained section. Ren? J. Pam Bakken wrote: From rjbuesa <@t> yahoo.com Wed May 14 14:21:40 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 14 14:21:42 2008 Subject: [Histonet] Salary Scales In-Reply-To: Message-ID: <623891.86971.qm@web65712.mail.ac4.yahoo.com> Which is equivalent to $26.20 / hour (2080 hours / year) and could be considered as "decent". Ren? J. JR R wrote: From mrsseagle <@t> yahoo.com Wed May 14 14:28:43 2008 From: mrsseagle <@t> yahoo.com (MICHELLE SEAGLE) Date: Wed May 14 14:28:49 2008 Subject: [Histonet] Suggestions on Equipment?? Message-ID: <739926.38110.qm@web51803.mail.re2.yahoo.com> Our hospital is currently looking to purchase a new cryostat, H& E Stainer and an Embedding Center. Any suggestions on a certain model,type or company with one that you have had a good experience with would be greatly appreciated. Michelle Seagle HT (ASCP) Rutherford Hospital MICHELLE SEAGLE From TBritten <@t> aol.com Wed May 14 14:36:57 2008 From: TBritten <@t> aol.com (TBritten@aol.com) Date: Wed May 14 14:37:09 2008 Subject: [Histonet] Suggestions on Equipment?? Message-ID: hello; we have used the "hacker" brand cryostat for many years at very high volumes and they have been very good. there are under a few brand marks but all who sell such things will be able to help you identify the latest model. tom In a message dated 5/14/2008 3:30:34 P.M. Eastern Daylight Time, mrsseagle@yahoo.com writes: Our hospital is currently looking to purchase a new cryostat, H& E Stainer and an Embedding Center. Any suggestions on a certain model,type or company with one that you have had a good experience with would be greatly appreciated. Michelle Seagle HT (ASCP) Rutherford Hospital MICHELLE SEAGLE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************Wondering what's for Dinner Tonight? Get new twists on family favorites at AOL Food. (http://food.aol.com/dinner-tonight?NCID=aolfod00030000000001) From pruegg <@t> ihctech.net Wed May 14 15:32:05 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed May 14 15:32:07 2008 Subject: [Histonet] cd38 on mouse tissue Message-ID: <003301c8b601$9378f720$6401a8c0@Patsyoffice> Anybody used cd38 on ffpe or frozen mouse tissue? Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From rjbuesa <@t> yahoo.com Wed May 14 15:40:16 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 14 15:40:20 2008 Subject: [Histonet] Suggestions on Equipment?? In-Reply-To: <739926.38110.qm@web51803.mail.re2.yahoo.com> Message-ID: <747728.50125.qm@web65703.mail.ac4.yahoo.com> Cryostat from Leica; H&E stainer and embedding center from Sakura, although it is very likely that the Leica people (that manufacture the 3 pieces of equipment) will give you a better price if you buy all from them. Otherwise follow the first choice (Leica + Sakura). Ren? J. MICHELLE SEAGLE wrote: From gayle.callis <@t> bresnan.net Wed May 14 16:46:23 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Wed May 14 16:46:26 2008 Subject: Sending privately Re: [Histonet] Glucose oxidase method for blocking endogenous peroxidase References: Message-ID: <000501c8b60b$f46ed9a0$6501a8c0@DHXTS541> Nadia, I will attach the method and all the regeant needs to you privately and where you can purchase the correct glucose. No, glucose D-(=) Glucose will NOT work. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Gale, Nadia" To: Sent: Wednesday, May 14, 2008 11:05 AM Subject: [Histonet] Glucose oxidase method for blocking endogenous peroxidase I've searched the archives and cannot find what I am after... I'd like to try the glucose oxidase method for blocking endogenous peroxidase in tissue sections for IHC. What is a suitable replacement for the B-d(+)glucose G5250 discontinued by Sigma? Will the D-(+)-Glucose G8270 work? This is 4% beta isomer and 96% alpha. Thanks for your help, Nadia Nadia Gale Lead Histotechnologist Centre for Translational and Applied Genomics (CTAG) 3427-600 West 10th Avenue Vancouver, BC V5Z 4E6 tel 604-877-6000 ext 2426 fax 604-877-6089 ngale@bccancer.bc.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cclutz2 <@t> life.uiuc.edu Wed May 14 17:12:59 2008 From: cclutz2 <@t> life.uiuc.edu (Claudia Lutz) Date: Wed May 14 17:13:17 2008 Subject: [Histonet] vibratome sections for cavalieri volume estimation In-Reply-To: References: Message-ID: <4769.130.126.50.22.1210803202.squirrel@www-s.life.uiuc.edu> Hi, I am fairly new to histological procedures and new to this list. My lab would like to stain vibratome sections of honey bee brain with phalloidin in order to estimate volume of specific brain regions. I am fixing the brains overnight in 4% paraformaldehyde, embedding in agarose, sectioning at 100 um, then staining free-floating sections in phalloidin with PBS with 0.2% Triton-X. I am mounting the slices in 80% glycerol and then imaging with the confocal microscope. I would like to use stereological procedures (specifically the cavalieri volume estimator) to quantify the volume of specific brain regions using systematic sampling of 10um optical sections that I obtain from the slices. I have several (possibly naive) questions: 1. My 100 um sections are shrinking to 60 um (or less) by the time I image them, judging by where I find or lose focus while imaging. What can I do to minimize this? How can I properly quantify the degree of shrinkage, to see whether or not it is uniform? Can I avoid bias in my volume estimation? 2. My phalloidin staining appears much brighter in the center of the slice, even when I use settings to adjust the gain while taking z-stacks. Does this indicate tissue damage at the edges? Can I ignore this? This also relates to my question above, since I am not sure I can precisely find the edge of the slice by focusing when I need to increase the gain. 3. I lose order and orientation of the slices because I am staining them free-floating. Does anyone have experience staining 40 or 50 um vibratome slices on the slide with phalloidin or antibody, or have a trustworthy protocol for this? Sorry to ask so many questions, and thank you for any help. There is not a lot of expertise in my lab on this, so I am feeling frustrated. Claudia Lutz University of Illinois at Urbana-Champaign Neuroscience Graduate Program From gayle.callis <@t> bresnan.net Wed May 14 17:22:26 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Wed May 14 17:22:29 2008 Subject: [Histonet] ZYMED Histostain Plus Kit on animal tissue References: <654253.27119.qm@web50308.mail.re2.yahoo.com> Message-ID: <003601c8b610$fd760000$6501a8c0@DHXTS541> Kim, This kit detects (in its broad spectrum) - mouse, rabbit, rat and guinea pig. You are correct about this more universal, multilink secondary. The kit protocol is found on the Invitrogen website with information on what is in the secondary spectrum. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Kim Merriam" To: "Mary L Giebel/FS/VCU" ; Sent: Wednesday, May 14, 2008 11:51 AM Subject: Re: [Histonet] ZYMED Histostain Plus Kit on animal tissue I dont have experience with this particular kit from Zymed, but the term "broad spectrum" makes me think that it would be similar to a universal secondary (that might include anti-mouse and anti-rabbit secondaries) which would likely cross-react to your rat tissue. It might be worth giving Zymed a call to find out about this kit. Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: Mary L Giebel/FS/VCU To: histonet@lists.utsouthwestern.edu Sent: Wednesday, May 14, 2008 12:56:51 PM Subject: [Histonet] ZYMED Histostain Plus Kit on animal tissue At a client's request, I am using a ZYMED's Broad Spectrum Histostain Plus Kit (#85-9843) on rat tissue. I have encountered an unexpected staining pattern as well as more background staining than usual. I would be very interested in hearing from others who have used this kit on animal tissue. I found the question posed in the Histonet archives (8/23/2005), but never found an answer posted. Thank you for your help. Regards, Mary Giebel _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shupeiwu <@t> umich.edu Wed May 14 17:31:31 2008 From: shupeiwu <@t> umich.edu (shupeiwu@umich.edu) Date: Wed May 14 17:31:34 2008 Subject: [Histonet] OCT vs. isopentane Message-ID: <20080514183131.39132e3x7n46szfo@web.mail.umich.edu> Hello all, I am quite new in the field of histochemistry. I'm doing mouse intestine cryostat section right now. I'm so confused about the method for cryostat section. First, I knew that most of time people using OCT to embed their sample, but I saw some papers they use liquid nitrogen cooled isopentane to embed their sample. Do these two methods cause any difference? Because I saw some background flurorescence in the OCT embedded slide. And if the sample embedded in isopentane, does the cryostat section take place in the same kind of machine? (with -20oC cryosection) Second, for the 4% Paraformaldehyde following by sucrose-PBS for cryoprotection, does it is really important to wash out the previous medium before entering the next step until the embedding. Third, does the thickness of the slide affect the autofluorescence? I knew those questions might some kind of vague. But thank you very much for any answers ahead. Wu From amosbrooks <@t> gmail.com Wed May 14 17:42:54 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed May 14 17:43:07 2008 Subject: [Histonet] Standardized Microtomes In-Reply-To: <211778.92149.qm@web65707.mail.ac4.yahoo.com> References: <582736990805131710s398184e7oaa2d5d3ae40e4b43@mail.gmail.com> <211778.92149.qm@web65707.mail.ac4.yahoo.com> Message-ID: <582736990805141542i42937995ub1411468c2feda9b@mail.gmail.com> Sorry Rene, I absolutly disagree with that. The exact opposite: *Not* modifying the angle of your block holder to suit the block, is not good practice. You are bound to loose tissue when cutting archived blocks, or blocks from other labs or even slight variations within your own lab. If you don't adapt to the blockyou are cutting. Without modifying the angle of the holder, one has 2 choices: Just cut thru it (refacing) or re-embedding and thereby needing to re-trim. Both scenerios will loose tissue. Often I have cut a freshly embedded block where the cassette didn't sit perfectly flat on the mold, otherwise the embedding was perfect. (Often due to poorly dissected tissue with high points). This results in an angle that is slightly askew. Adjusting the block holder is much faster than re-embedding the block. If it is done properly you can actually modify the block angle without ever cutting any tissue even on a previously cut block. We're going to have to disagree on this, Amos On Wed, May 14, 2008 at 9:14 AM, Rene J Buesa wrote: > All in all the practice of "changing the angle to match the block you have > to recut as needed" is not a good practice. > During it you could end loosing tissue when trying to "build" a new flat > surface from a block that was originally cut at a different angle, while > "sculping" the new surface. > The best practice is to have all the microtomes cutting at the same angle > and try to convince those HTs that say that "I have always cut with this > angle" that they are flat wrong and a general angle can be good for the > majority of blocks. > Ren? J. > From PMonfils <@t> Lifespan.org Wed May 14 17:57:17 2008 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed May 14 17:57:22 2008 Subject: [Histonet] OCT vs. isopentane In-Reply-To: <20080514183131.39132e3x7n46szfo@web.mail.umich.edu> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273D7B@LSRIEXCH1.lsmaster.lifespan.org> Isopentane is not an embedding medium. OCT (or another comparable brand) is the embedding medium. Isopentane cooled with liquid nitrogen or dry ice is the freezing medium for freezing the liquid OCT into solid blocks. In sucrose cryoprotection, some people wash out the formalin before going into the first sucrose solution. I find that the sucrose solution itself washes out the formalin quite adequately. You do not wash at all between the different concentrations of sucrose. That would defeat the purpose of using an ascending series of concentrations. From gayle.callis <@t> bresnan.net Wed May 14 18:31:20 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Wed May 14 18:31:23 2008 Subject: [Histonet] Standardized Microtomes References: <582736990805131710s398184e7oaa2d5d3ae40e4b43@mail.gmail.com><211778.92149.qm@web65707.mail.ac4.yahoo.com> <582736990805141542i42937995ub1411468c2feda9b@mail.gmail.com> Message-ID: <006801c8b61a$9da832b0$6501a8c0@DHXTS541> When recutting a block or having one where the embedded tissue is a bit out whack for decent cutting alignment, then the x/y axis IS adjusted. The blade angle is generally not adjusted, only the x/y axis is changed. Even moving a blade sideways to access a sharp, new edge can alter the x/y orientation slightly. When this happens, reapproaching the blade, x/y adjustement may be needed to get first sections coming off the block - often the sections one absolutely must have. We don't change the blade angle unless someone tweaks the lever (generally an inexperienced tech or student who has no clue what is going on but they love flipping levers around). Sometimes a blade angle is readjusted from a new blade lot even though the manufacturer of the blade is the same. The blade manufacturing process can introduce minute changes in blades. When changing from high to low profile blades we readjust blade angle slightly at times - the low profiles being thinner in metal thickness and narrower width from top to bottom than the high profile. Rembedding and refacing a block is not ideal. As Amos points out, the danger of losing the region of interest in the embedded tissue is just too high especially on a recut. One can always turn a block in the holder, but that means some clever, careful x/y axis adjustment - we do this all the time. I vote for Amos's way as he just described it, and that is how we do it in our lab. We meaning "I" do it, the x/y axis way. The lever tweakers are generally gently repirmanded and taught the correct way for where a blade angle works best for the preferred blade in the lab. Gayle M. Callis HTL/HT/MT(ASCP) Bozemant MT ----- Original Message ----- From: "Amos Brooks" To: "Rene J Buesa" Cc: Sent: Wednesday, May 14, 2008 4:42 PM Subject: Re: [Histonet] Standardized Microtomes Sorry Rene, I absolutly disagree with that. The exact opposite: *Not* modifying the angle of your block holder to suit the block, is not good practice. You are bound to loose tissue when cutting archived blocks, or blocks from other labs or even slight variations within your own lab. If you don't adapt to the blockyou are cutting. Without modifying the angle of the holder, one has 2 choices: Just cut thru it (refacing) or re-embedding and thereby needing to re-trim. Both scenerios will loose tissue. Often I have cut a freshly embedded block where the cassette didn't sit perfectly flat on the mold, otherwise the embedding was perfect. (Often due to poorly dissected tissue with high points). This results in an angle that is slightly askew. Adjusting the block holder is much faster than re-embedding the block. If it is done properly you can actually modify the block angle without ever cutting any tissue even on a previously cut block. We're going to have to disagree on this, Amos On Wed, May 14, 2008 at 9:14 AM, Rene J Buesa wrote: > All in all the practice of "changing the angle to match the block you have > to recut as needed" is not a good practice. > During it you could end loosing tissue when trying to "build" a new flat > surface from a block that was originally cut at a different angle, while > "sculping" the new surface. > The best practice is to have all the microtomes cutting at the same angle > and try to convince those HTs that say that "I have always cut with this > angle" that they are flat wrong and a general angle can be good for the > majority of blocks. > Ren? J. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jstaruk <@t> masshistology.com Wed May 14 19:02:15 2008 From: jstaruk <@t> masshistology.com (jstaruk) Date: Wed May 14 19:02:14 2008 Subject: [Histonet] Help identifying LS174T tumor cells In-Reply-To: <734.60655.qm@web65716.mail.ac4.yahoo.com> Message-ID: OK, I've been working on this for over a week and finally decided I need help. LS174T tumor cells (a human colon cancer cell line) were implanted into mouse lymph nodes. I'm trying to immuno-stain these implanted cells. I tried a pan cytokeratin antibody and a CK20 cytokeratin antibody and neither worked. Has anyone here ever worked with this cell line? Any suggestions on how to differentiate these cells from the normal mouse cells? Thanks! Jim _____________________ Jim Staruk Mass Histology Service www.masshistology.com From gamal.akabani <@t> gmail.com Wed May 14 20:09:26 2008 From: gamal.akabani <@t> gmail.com (Gamal Akabani) Date: Wed May 14 20:09:34 2008 Subject: [Histonet] Gamal Akabani has sent you a hi5 Friend Request Message-ID: <1478199894.95.1210813766874.JavaMail.root@sfapp294> Gamal Akabani would like to be your friend on hi5! I set up a hi5 profile and I want to add you as a friend so we can share pictures and start building our network. First you need to join hi5! Once you join, you will have a chance to create a profile, share pictures, and find friends. Thanks, Gamal [1]Join hi5!» [2][nophoto_boy_100.gif] [3]Gamal Akabani 1 Friends ------------------------------------------------------ Copyright 2002-2008 Hi5 Networks, Inc. All rights reserved. P.O. Box 31118, San Francisco, CA 94131, USA [4]Privacy Policy | [5]Unsubscribe | [6]Terms of Service [to.do?loginid=&smid=] References 1. http://www.hi5.com/friend/registration/displayRegistration.do?inviteId=B_e6054e2_ZwJcpc6CJdh&smid= 2. http://www.hi5.com/friend/registration/displayRegistration.do?inviteId=B_e6054e2_ZwJcpc6CJdh&smid= 3. http://www.hi5.com/friend/registration/displayRegistration.do?inviteId=B_e6054e2_ZwJcpc6CJdh&smid= 4. http://www.hi5.com/friend/displayPrivacy.do 5. http://www.hi5.com/friend/account/displayEditPrivacy.do?loginid= 6. http://www.hi5.com/friend/displayTOS.do From koellingr <@t> comcast.net Wed May 14 20:46:09 2008 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Wed May 14 20:46:14 2008 Subject: [Histonet] Help identifying LS174T tumor cells Message-ID: <051520080146.9807.482B95E1000D52F40000264F22134843739D09020704040A0105@comcast.net> Jim, Haven't worked on that cell line in lymph nodes but in other parts of mice, xenografts and monolayers. I'm assuming these are nude mice so the cells aren't simply being destroyed by host (mouse) immune response. I'd stay away from cytokeratins. CK20 can be downregulated in that line and there are cytokeratins that endogenously mark in lymph nodes. That line I believe is presumed to be goblet cell associated. I've looked at it with CEA and villin, things other than CK20 or pan cytokeratin that might traditionally mark colon. Might try cdx2 besides CEA or villin. I believe in ATTC that line is known as a large producer of CEA and state of the art (back when I did this) was to localize LS174T's was radiolabelled CEA. Never tried but MUC antibody or even a PAS or mucin histology stain might do. Raymond Koelling PhenoPath Labs Seattle, WA -------------- Original message -------------- From: "jstaruk" > OK, I've been working on this for over a week and finally decided I need > help. LS174T tumor cells (a human colon cancer cell line) were implanted > into mouse lymph nodes. I'm trying to immuno-stain these implanted cells. > I tried a pan cytokeratin antibody and a CK20 cytokeratin antibody and > neither worked. Has anyone here ever worked with this cell line? Any > suggestions on how to differentiate these cells from the normal mouse cells? > > Thanks! > > Jim > > _____________________ > Jim Staruk > Mass Histology Service > www.masshistology.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jim.manavis <@t> imvs.sa.gov.au Wed May 14 22:14:08 2008 From: jim.manavis <@t> imvs.sa.gov.au (Jim Manavis) Date: Wed May 14 22:14:23 2008 Subject: FW: [Histonet] Help identifying LS174T tumor cells Message-ID: <001c01c8b639$bd8796b0$636c140a@itp36533> Jim I've gone through something like this a few years ago. We attempted to differentiate between host mouse cells and introduced human cancer cells. We used the human mitochondrial marker successfully on FFPE tissue to differentiate between the two. Initially we were using the Chemicon antibody (Cat No. MAB1273) but then struck a snag with it, where with a particular set of batch numbers it didn't work. We then turned to the Abcam antibody (Cat No. ab3298) and have been using this ever since Cheers Jim Jim Manavis Laboratory Manager Hanson Institute Centre for Neurological Diseases IMVS, Adelaide, SA, 5000 Australia Phone: 61-08-8222-3668 / 0401120697 FAX: 61-08-8222 3392 email: jim.manavis@imvs.sa.gov.au Disclaimer: Not this little black duck! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of koellingr@comcast.net Sent: Thursday, 15 May 2008 11:16 AM To: jstaruk; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Help identifying LS174T tumor cells Jim, Haven't worked on that cell line in lymph nodes but in other parts of mice, xenografts and monolayers. I'm assuming these are nude mice so the cells aren't simply being destroyed by host (mouse) immune response. I'd stay away from cytokeratins. CK20 can be downregulated in that line and there are cytokeratins that endogenously mark in lymph nodes. That line I believe is presumed to be goblet cell associated. I've looked at it with CEA and villin, things other than CK20 or pan cytokeratin that might traditionally mark colon. Might try cdx2 besides CEA or villin. I believe in ATTC that line is known as a large producer of CEA and state of the art (back when I did this) was to localize LS174T's was radiolabelled CEA. Never tried but MUC antibody or even a PAS or mucin histology stain might do. Raymond Koelling PhenoPath Labs Seattle, WA -------------- Original message -------------- From: "jstaruk" > OK, I've been working on this for over a week and finally decided I need > help. LS174T tumor cells (a human colon cancer cell line) were implanted > into mouse lymph nodes. I'm trying to immuno-stain these implanted cells. > I tried a pan cytokeratin antibody and a CK20 cytokeratin antibody and > neither worked. Has anyone here ever worked with this cell line? Any > suggestions on how to differentiate these cells from the normal mouse cells? > > Thanks! > > Jim > > _____________________ > Jim Staruk > Mass Histology Service > www.masshistology.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From annigyg <@t> gmail.com Wed May 14 23:05:05 2008 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Wed May 14 23:05:09 2008 Subject: [Histonet] Suggestions on Equipment?? In-Reply-To: <747728.50125.qm@web65703.mail.ac4.yahoo.com> References: <739926.38110.qm@web51803.mail.re2.yahoo.com> <747728.50125.qm@web65703.mail.ac4.yahoo.com> Message-ID: it seems like Rene and I were cut from a similar AP mould Leica Cryotat - go for the new one with the on board decontamination Sakura Embedder - its for left handers and right handers and the cold plate is a separate unit - neat and compact Sakura DRS2000 or the Prisma (with the attached coverslipper) - it has small staining dishes (optional) which i just love Sakura need to vamp up their Cryostat technology - then my whole lab will be a showcase of their products - are you reading this guys - LOL Annie 2008/5/15 Rene J Buesa : > Cryostat from Leica; H&E stainer and embedding center from Sakura, although > it is very likely that the Leica people (that manufacture the 3 pieces of > equipment) will give you a better price if you buy all from them. Otherwise > follow the first choice (Leica + Sakura). > Ren? J. > > MICHELLE SEAGLE wrote: > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From rjbuesa <@t> yahoo.com Thu May 15 07:43:21 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 15 07:43:25 2008 Subject: [Histonet] Standardized Microtomes In-Reply-To: <582736990805141542i42937995ub1411468c2feda9b@mail.gmail.com> Message-ID: <812281.7705.qm@web65711.mail.ac4.yahoo.com> Amos: You had pointed out to the "acceptable exception". I do not advocate to NEVER align a blade to a block when necessary. What I say that for new block to be cut ALL microtomes in the lab should use the same angle and allow, for instance, that any HT could cut a recur for any special procedure without having to adjust the cutting angle to that specific block, because it was cut with an uniform angle. Archival blocks, or those received from another institution as a consult, of course that should be cut by adapting the cutting angle to the block, that is always better than reembedding the block. So, for NEW, everyday routine blocks: all microtomes with the same angle. For old or consult blocks: adapt the microtome to them. Besides, you don't have to be sorry to express your opinion! Ren? J. From Bonnie.Whitaker <@t> osumc.edu Thu May 15 08:00:32 2008 From: Bonnie.Whitaker <@t> osumc.edu (Whitaker, Bonnie) Date: Thu May 15 08:00:48 2008 Subject: [Histonet] Looking for Melissa Barrett Message-ID: <3CE20ED86C4A114EBDF3BCE8DEFD8F60262493@msxc06.OSUMC.EDU> Hi All, We are trying to locate Melissa Barrett (at one time she was at the South Bend Medical Foundation) regarding a revision and repackaging of an exercise she did for TechSample a few years back. Melissa, if you are here please email me with contact info, or if anyone else knows how to reach her, please let me know. Thanks, Bonnie Whitaker Histology Manager Ohio State University Medical Center 614.293.5048 From nrutledge <@t> CapeCodHealth.org Thu May 15 09:33:45 2008 From: nrutledge <@t> CapeCodHealth.org (Rutledge, Nancy) Date: Thu May 15 09:35:05 2008 Subject: [Histonet] need knife guard Message-ID: <47AD3B259E920D449F580E6AE82C2B8F210256@FHEXSVR2.FHDOMAIN1.capecodhealth.org> I'm looking for one of the old knife guards, metal clip, fits over blade that extends beyond knife holder. Happy to pay for it, only need one. Thanks Nancy Rutledge Falmouth Hospital 100 Ter Heun Drive Falmouth, MA 02540 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org From GDawson <@t> dynacaremilwaukee.com Thu May 15 09:59:48 2008 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Thu May 15 09:59:53 2008 Subject: [Histonet] Salary Scales In-Reply-To: <623891.86971.qm@web65712.mail.ac4.yahoo.com> Message-ID: $26.20 / hour is "decent"? For the Milwaukee area, that is a phenominal payrate. What regions see this as "run of the mill"? I wish I could offer techs this pay. It would certainly solve all of our staffing issues in one fell swoop. I also wonder where payscales like this are when the Histology pay scales are being compiled for publication. You look at our printed ranges and you cannot help but think that they are more useful to those who want to keep histo-pay low. Call me a coward but I don't feel comfortable quoting our average histotech pay. I'll just say that it is SIGNIFICANTLY below $26.20. Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Wednesday, May 14, 2008 2:22 PM To: JR R; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Salary Scales Which is equivalent to $26.20 / hour (2080 hours / year) and could be considered as "decent". Ren? J. JR R wrote: _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu May 15 11:18:00 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 15 11:18:07 2008 Subject: [Histonet] Salary Scales In-Reply-To: Message-ID: <355672.50007.qm@web65701.mail.ac4.yahoo.com> Glen: And it will keep that way until histotechs star demanding what is deserved! Have you realized that histotechs are the only specialists in the medical lab that have to make decisions all along the process? When to reject a too thick slice of tissue to assure proper processing? What part to embed to cut? Up to where trim the block discarding parts of the specimen FOR EVER?! Which section to take or which to discard FOR EVER?! When to stop differentiation in a special stain? There is no other area of the ML that has to take so many decisions, and they are better paid. And will be until the HTs decide to take action and demand what is deserved. Just my opinion (as usual!). Ren? J. "Dawson, Glen" wrote: From rhdes.street <@t> googlemail.com Thu May 15 11:39:02 2008 From: rhdes.street <@t> googlemail.com (Rupert Street) Date: Thu May 15 11:39:09 2008 Subject: [Histonet] Alternative Preserving Medium for Histology Message-ID: <2e02b98e0805150939y2eca73dcld8413d92c742e9d6@mail.gmail.com> Dear All; At the risk of running the gamut with your news group, and I have asked permission. Myself and my colleagues are looking for people interested in beta testing an alternative to formalin/formaldehyde for within the histopathology industry. Before you start cracking back with questions, this product is not a fixative so therefore is no use at all for long term preservation. It is purely focussed towards biopsy usage. The product has been adapted from a successful product used within the funeral industry which seeks to replace formalin based embalming wherever people have concerns etc. If you are interested in helping us please send your details to the attached e-mail. Regards Rupert Street From Erin.Martin <@t> ucsf.edu Thu May 15 11:51:29 2008 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Thu May 15 11:52:30 2008 Subject: [Histonet] Salary Scales Message-ID: $26/hr for an experienced tech is in range here... From ktuttle <@t> umm.edu Thu May 15 11:58:15 2008 From: ktuttle <@t> umm.edu (Kimberly Tuttle) Date: Thu May 15 11:58:39 2008 Subject: [Histonet] Cutting research blocks from home? In-Reply-To: <482ADD9D.37F9.0000.0@childrensmn.org> References: <482ADD9D.37F9.0000.0@childrensmn.org> Message-ID: <482C3366.90CE.001A.3@umm.edu> Is there anyone on the list who does this? How is a "basement lab" regulated? If you dont do any staining and only cut unstained, therefore no reagents, is there no need to be regulated? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax >>> "Pam Bakken" 5/14/2008 1:39:57 pm >>> Hi, I am interested in working part time from home cutting research blocks. Is there anyone that could give me some advice on how to get started? How do I charge? Any experience, advice or knowledge would be greatly appreciated. Thanks, Pamm Bakken Children's Hospital - Minneapolis _______________________________________________________________ Confidentiality Statement: This email/fax, including attachments, may include confidential and/or proprietary information and may be used only by the person or entity to which it is addressed. If the reader of this email/fax is not the intended recipient or his or her agent, the reader is hereby notified that any dissemination, distribution or copying of this email/fax is prohibited. If you have received this email/fax in error, please notify the sender by replying to this message and deleting this email or destroying this facsimile immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From Heather.D.Renko <@t> osfhealthcare.org Thu May 15 12:19:47 2008 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Thu May 15 12:20:02 2008 Subject: [Histonet] re: Leica IPC Message-ID: <40026EDDE64CDA47AB382C52619ACD3C0A09DF3D@pmc-rfd-mx01.intranet.osfnet.org> For any Leica cassette labeler IPC users, I am curious how often are you gong through ink cartridges and what is you volume. I am trying to estimate annual costs. Please reply directly to me and thank you for your input. Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From Traczyk7 <@t> aol.com Thu May 15 12:29:51 2008 From: Traczyk7 <@t> aol.com (Traczyk7@aol.com) Date: Thu May 15 12:30:02 2008 Subject: [Histonet] Veronica "Ronnie" Alzheimer Message-ID: I just found out that Ronnie Alzheimer died on May 11, 2008. She was the director of the histology program at Muhlenberg Hospital in Plainfield, NJ from about 1975 to 1985. She spent 15 years as director of education at St. Francis Medical Center in Trenton, NJ. I remember her fondly as a dedicated educator and friend. I can honestly say that I would not have remained in the histology field if I did not have the opportunity to be part of the program that Ronnie ran at Muhlenberg. In lieu of flowers, donations to the American Lung Association (1600 Route 22 East Union, NJ 07083) have been requested by her family. I will pass along any cards or emails if you send them to me at _murp593@aol.com_ (mailto:murp593@aol.com) or 529 Smith Drive Pt. Pleasant, NJ 08742. Regards, Dorothy Murphy Traczyk **************Wondering what's for Dinner Tonight? Get new twists on family favorites at AOL Food. (http://food.aol.com/dinner-tonight?NCID=aolfod00030000000001) From Karen.Heckford <@t> CHW.edu Thu May 15 12:32:18 2008 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Thu May 15 12:32:23 2008 Subject: [Histonet] Salary Scales In-Reply-To: References: <623891.86971.qm@web65712.mail.ac4.yahoo.com> Message-ID: <2842DC75AE43AA4B92954CFB31781BC1821654@CHW-MSG-301.chw.edu> I guess it does really depend on the area. In San Francisco it is high twenties to mid thirties. One hour north of San Francisco it can be as low as $7-10.00 an hour less. Big jump for 70 miles. Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathology Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Thursday, May 15, 2008 8:00 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Salary Scales $26.20 / hour is "decent"? For the Milwaukee area, that is a phenominal payrate. What regions see this as "run of the mill"? I wish I could offer techs this pay. It would certainly solve all of our staffing issues in one fell swoop. I also wonder where payscales like this are when the Histology pay scales are being compiled for publication. You look at our printed ranges and you cannot help but think that they are more useful to those who want to keep histo-pay low. Call me a coward but I don't feel comfortable quoting our average histotech pay. I'll just say that it is SIGNIFICANTLY below $26.20. Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Wednesday, May 14, 2008 2:22 PM To: JR R; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Salary Scales Which is equivalent to $26.20 / hour (2080 hours / year) and could be considered as "decent". Ren? J. JR R wrote: _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmiller <@t> physlab.com Thu May 15 12:38:31 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Thu May 15 12:37:22 2008 Subject: [Histonet] FW: Positive staining on my neg controls Message-ID: <000001c8b6b2$7e4b09e0$3d02a8c0@plab.local> Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 _____ From: Cheri Miller [mailto:cmiller@physlab.com] Sent: Thursday, May 15, 2008 11:52 AM To: histonet-bounces@lists.utsouthwestern.edu Subject: Positive staining on my neg controls I am having blush of staining on my mono neg controls.I have a nexus IHC from ventana.I do my retrieval off line with a steamer. I have not had this issue before, it has happened with 3 antibodies so far..any suggestions? Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From tim.morken <@t> thermofisher.com Thu May 15 12:42:28 2008 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Thu May 15 12:43:07 2008 Subject: [Histonet] Fingernail IHC problems Message-ID: <6BFF6D137DF6BC43B33891BA96E83B190176DC9A@PGHCR-EXMB-VS-1.na.fshrnet.com> I had a quesiton about IHC on fingernail treated with KOH to soften it. Are any antigens significantly or completely destroyed by KOH treatment? Are there any restoration treatments? Is there something else that is better for softening that will not harm IHC? Thanks! Tim Morken Technical Support Manager Lab Vision Products Anatomical Pathology ThermoFisher Scientific From pkromund <@t> gundluth.org Thu May 15 13:11:19 2008 From: pkromund <@t> gundluth.org (pkromund@gundluth.org) Date: Thu May 15 13:11:36 2008 Subject: [Histonet] Salary Scales In-Reply-To: Message-ID: wherer is (here)? "Martin, Erin" To Sent by: "histonet" histonet-bounces@ lists.utsouthwest cc ern.edu Subject RE: [Histonet] Salary Scales 05/15/2008 11:51 AM $26/hr for an experienced tech is in range here... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From am3309 <@t> uga.edu Thu May 15 13:11:49 2008 From: am3309 <@t> uga.edu (Abigail M. Butler) Date: Thu May 15 13:11:53 2008 Subject: [Histonet] Combined Ag/AChE histochemical staining Message-ID: <20080515141149.OBS34199@punts1.cc.uga.edu> Can anyone give any information about doing a combined Ag/AChE histochemical stain on frozen sections? Thanks Abbie Butler, HT (ASCP), QIHC University of Georgia College of Veterinary Medicine From sharon.osborn <@t> comcast.net Thu May 15 13:16:01 2008 From: sharon.osborn <@t> comcast.net (sharon.osborn@comcast.net) Date: Thu May 15 13:16:34 2008 Subject: [Histonet] Ford Royer Message-ID: <051520081816.26667.482C7DE1000C95D40000682B2216566276029D010D9C01D202019D0E089C@comcast.net> Ford, please send me your current contact information. Thank you, sharon osborn LabVision ThermoFisher 47777 Warm Springs Blvd Fremont, CA 510-991-2858 From pruegg <@t> ihctech.net Thu May 15 13:22:58 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu May 15 13:23:12 2008 Subject: [Histonet] Salary Scales In-Reply-To: <2842DC75AE43AA4B92954CFB31781BC1821654@CHW-MSG-301.chw.edu> References: <623891.86971.qm@web65712.mail.ac4.yahoo.com> <2842DC75AE43AA4B92954CFB31781BC1821654@CHW-MSG-301.chw.edu> Message-ID: <007d01c8b6b8$b7ce7de0$6401a8c0@Patsyoffice> In Colorado it depends on if you are permanently employed or working per diem, The daily people who cover and get no benefits make at least $25 per hour, but those employed with benefits go from $15 up an hour. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Thursday, May 15, 2008 11:32 AM To: Dawson, Glen; Histonet (E-mail) Subject: RE: [Histonet] Salary Scales I guess it does really depend on the area. In San Francisco it is high twenties to mid thirties. One hour north of San Francisco it can be as low as $7-10.00 an hour less. Big jump for 70 miles. Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathology Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Thursday, May 15, 2008 8:00 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Salary Scales $26.20 / hour is "decent"? For the Milwaukee area, that is a phenominal payrate. What regions see this as "run of the mill"? I wish I could offer techs this pay. It would certainly solve all of our staffing issues in one fell swoop. I also wonder where payscales like this are when the Histology pay scales are being compiled for publication. You look at our printed ranges and you cannot help but think that they are more useful to those who want to keep histo-pay low. Call me a coward but I don't feel comfortable quoting our average histotech pay. I'll just say that it is SIGNIFICANTLY below $26.20. Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Wednesday, May 14, 2008 2:22 PM To: JR R; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Salary Scales Which is equivalent to $26.20 / hour (2080 hours / year) and could be considered as "decent". Ren? J. JR R wrote: _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wilson_c <@t> ricerca.com Thu May 15 13:23:11 2008 From: wilson_c <@t> ricerca.com (Wilson, Carol) Date: Thu May 15 13:23:15 2008 Subject: [Histonet] nerve dissection and section of rodents Message-ID: <9D443EB9D0270143B5AAF190CB1A58A3067963BE@dogwood.ricerca.com> Hi All, Looking for any helpful hints or suggestions in obtaining good sections at both dissection and microtomy of ganglia/roots along spinal cord of rats, as well as peroneal, sural and tibial nerves. Routine paraffin processing with H&E staining. Thanks in advance, Carol Carol Wilson, HT(ASCP) Lead Technician/Histology Ricerca Biosciences, LLC 440-357-3930 From emerald_lake77 <@t> yahoo.com Thu May 15 13:24:50 2008 From: emerald_lake77 <@t> yahoo.com (GT Hebert) Date: Thu May 15 13:24:55 2008 Subject: [Histonet] Anti-mouse CD56 antibody Message-ID: <968547.73681.qm@web31706.mail.mud.yahoo.com> Hello, Does anyone know of a working anti-mouse CD56 antibody. I plan on using a mouse brain as the positive control. Any advice or information would be greatly appreciated. Thank you. Gustave Gustave T. Hebert Scientist II Cardiovascular and Metabolic Diseases Wyeth Research Cambridge MA From manningl <@t> inspection.gc.ca Thu May 15 15:49:20 2008 From: manningl <@t> inspection.gc.ca (Lisa Manning) Date: Thu May 15 15:49:31 2008 Subject: [Histonet] formalin tanks Message-ID: Dear Histonet group, Some of the hospitals here are using large formalin tanks. Does anyone have experience with these tanks and is there an SOP or any preventative maintenance involved with these tanks (ie. Treat yearly with CLR or other de-scaling solution)? Thanks, Lisa Lisa Manning MLT, BSc. Pathology Technical Director Diagnostic Services of Manitoba 401B Brodie Centre 727 McDermot Avenue Winnipeg, Manitoba R3E 3P5 Ph. (204) 789-3325 Fax (204) 789-3931 lmanning@hsc.mb.ca From ebreisch <@t> rchsd.org Thu May 15 15:54:34 2008 From: ebreisch <@t> rchsd.org (Breisch, Eric) Date: Thu May 15 15:54:41 2008 Subject: [Histonet] ATYPICAL MYCOBACTERIUM Message-ID: <43B97B4C402C2C44AAA2A8D2C86A88B31A5ABA@e2k3backend1.RCHSD.org> Is anyone employing an immunoperoxidase stain for quick identification of atypical mycobacterium and if so would they please share the antibody used, the commercial source and any other pertinent information which may be useful for employing this technique? Your help is greatly appreciated. Thank you, Eric Eric A. Breisch, Ph.D. Clinical Anatomist Dept. of Pathology Rady Children's Hospital and Health Center Associate Clinical Professor of Anatomy Dept. of Surgery UCSD School of Medicine From talulahgosh <@t> gmail.com Thu May 15 16:56:40 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu May 15 16:56:46 2008 Subject: [Histonet] Cutting research blocks from home? In-Reply-To: <482C3366.90CE.001A.3@umm.edu> References: <482ADD9D.37F9.0000.0@childrensmn.org> <482C3366.90CE.001A.3@umm.edu> Message-ID: On Thu, May 15, 2008 at 12:58 PM, Kimberly Tuttle wrote: > Is there anyone on the list who does this? How is a "basement lab" regulated? If you dont do any staining and only cut unstained, therefore no reagents, is there no need to be regulated? > I would look into the institutions from who(m?) you're getting the specimens. They might not let anyone take the tissue off of the facility grounds. With a university lab, we can't even move tissue to another lab without filling out forms. Also, human tissue is BSL-2, at least, right? Emily -- If trees could scream, would we be so cavalier about cutting them down? We might, if they screamed all the time, for no good reason. From tkngflght <@t> yahoo.com Thu May 15 16:56:48 2008 From: tkngflght <@t> yahoo.com (Cheryl) Date: Thu May 15 16:56:52 2008 Subject: [Histonet] Fresh frozen--which Gill Heme works better?? In-Reply-To: <051520080146.9807.482B95E1000D52F40000264F22134843739D09020704040A0105@comcast.net> References: <051520080146.9807.482B95E1000D52F40000264F22134843739D09020704040A0105@comcast.net> Message-ID: <00d301c8b6d6$9417bf60$320aa8c0@FSROGER> Hi All! We're working up a new shorter stain for our frozens. Which Gill hematoxyin would you suggest for dermatology frozen sections? We like a progressive stain that blues well with ammonia water. Gill I or Gill II? Cheryl Cheryl R. Kerry, HT(ASCP), BA Full Staff Inc. Staffing the lab - One GREAT tech at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone admin@fullstaff.org www.fullstaff.org From Herrick.James <@t> mayo.edu Thu May 15 16:58:16 2008 From: Herrick.James <@t> mayo.edu (Herrick, James L.) Date: Thu May 15 16:58:21 2008 Subject: [Histonet] Question on epithelial staining in plastic resin Message-ID: Hello everyone, Does anyone have experience staining for epithelial cells (pig arteries) in MMA embedded specimens? So far I have run immuno for VWF, eNOS and am currently trying a lectin stain using Griffonia (no success). I have previously run immuno on this same tissue type for actin which looked great. I am using a steamer for antigen retrieval with the solution Retrievit-8 from InnoGenex. I have tried anywhere from 15 minutes to 1 hour in the steamer. For deplastification I am using a Xylene, 2-MEA, Acetone and ETOH protocol. I am thinking that this may be too harsh. Any feedback on this issue would be greatly appreciated. Thanks for your help. Jim From gayle.callis <@t> bresnan.net Thu May 15 17:49:40 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Thu May 15 17:49:43 2008 Subject: [Histonet] Question on epithelial staining in plastic resin References: Message-ID: <000e01c8b6dd$f661c790$6501a8c0@DHXTS541> Neil Hand did pressure cooker retrieval and published in J of Histotechnology, NM Hand, Chruch RJ. Superheating using pressure cooking: its use and application in unmasking antigens embedded in methyl methacrylate. J Histotechnology, 21:231-236, 1988. If you are a member of NSH, they will send a copy of this to you - not sure if it will cost you anything. NSH website has application for doing this, or call them. He also wrote a chapter on the subject in Gamble and Bancroft Theory and Practice of Histological Techniques, 6th Edition, 2007 chapter 29. He removed MMA from thin tissue sections, 2 um or so, with 37C xylene, 10 to 20 minutes, followed by a routine rehydration to distilled water. He had great success with his IHC on MMA embedded tissues, with a panel of 200 antibodies or so. I am sure the thickness of the section will affect MMA removal, so time in the warm xylene may need to be extended and maybe an extra change to ensure plastic removal. I am not sure if he has pdf's of his publications, but you can contact him at Hand Neil (Histopathology): Neil.Hand@nuh.nhs.uk He is regarded as one of the experts for doing MMA/IHC work. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Herrick, James L." To: Sent: Thursday, May 15, 2008 3:58 PM Subject: [Histonet] Question on epithelial staining in plastic resin Hello everyone, Does anyone have experience staining for epithelial cells (pig arteries) in MMA embedded specimens? So far I have run immuno for VWF, eNOS and am currently trying a lectin stain using Griffonia (no success). I have previously run immuno on this same tissue type for actin which looked great. I am using a steamer for antigen retrieval with the solution Retrievit-8 from InnoGenex. I have tried anywhere from 15 minutes to 1 hour in the steamer. For deplastification I am using a Xylene, 2-MEA, Acetone and ETOH protocol. I am thinking that this may be too harsh. Any feedback on this issue would be greatly appreciated. Thanks for your help. Jim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From klmercer <@t> MIT.EDU Fri May 16 06:53:04 2008 From: klmercer <@t> MIT.EDU (Kim Mercer) Date: Fri May 16 06:53:33 2008 Subject: [Histonet] cadenza buffer alternative or recipe Message-ID: Thermo Fisher are not making Cadenza Buffer Wash Concentrate( cat #407340) that is used with the Sequenza coverplate system for immunohistochemistry. Thermo Fisher did recommend Tris buffered saline with Tween but we have found that our immunohostochemisty is not as nice as it was with cadenza. According to the MSDS Cadenza contains sodium chloride, Tris and Polyoxyethylene. Does anyone have any suggestions or advice in regard to a cadenza replacement? Thermo-fisher won't tell us the recipe. Thanks Kim Mercer Koch Institute for Integrative Cancer Research From froyer <@t> bitstream.net Fri May 16 08:29:56 2008 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri May 16 08:30:08 2008 Subject: [Histonet] Ford Royer In-Reply-To: <051520081816.26667.482C7DE1000C95D40000682B2216566276029D010D9C01D202019D0E089C@comcast.net> References: <051520081816.26667.482C7DE1000C95D40000682B2216566276029D010D9C01D202019D0E089C@comcast.net> Message-ID: <004801c8b758$ee8444f0$7701a80a@Ford> Here is my contact information.... Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL:? 612-839-1046 Phone:? 763-542-8725 Fax:? 763-546-4830 eMail:? froyer@bitstream.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sharon.osborn@comcast.net Sent: Thursday, May 15, 2008 1:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ford Royer Ford, please send me your current contact information. Thank you, sharon osborn LabVision ThermoFisher 47777 Warm Springs Blvd Fremont, CA 510-991-2858 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mona_diane <@t> hotmail.com Fri May 16 08:45:32 2008 From: mona_diane <@t> hotmail.com (Ramona Turner) Date: Fri May 16 08:45:40 2008 Subject: [Histonet] Celestine Blue Procedure Message-ID: Does anyone have a tried and true procedure using celestine blue in place of hematoxylin? I am contemplating using celestine blue in place of hematoxylin if it becomes scarce. I tried Celestine blue in place of hematoxlyin on my stainer using the usual times, but the results where not exactly the same. It needs some squeeking. Thanks for the help. Ramona _________________________________________________________________ Keep your kids safer online with Windows Live Family Safety. http://www.windowslive.com/family_safety/overview.html?ocid=TXT_TAGLM_WL_Refresh_family_safety_052008 From AFeatherstone <@t> KaleidaHealth.Org Fri May 16 09:07:03 2008 From: AFeatherstone <@t> KaleidaHealth.Org (Featherstone, Annette) Date: Fri May 16 09:07:17 2008 Subject: [Histonet] RE: Histonet Digest, Vol 54, Issue 21 In-Reply-To: References: Message-ID: <16A5E67B2A1F714885DEDC2CB68DD6090CB8EB@KALEXMB03.KaleidaHealth.org> What is everyone doing about the hematoxylin shortage? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, May 15, 2008 12:48 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 54, Issue 21 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Glucose oxidase method for blocking endogenous peroxidase (Gale, Nadia) 2. Cutting research blocks from home? (Pam Bakken) 3. Re: ZYMED Histostain Plus Kit on animal tissue (Kim Merriam) 4. Re: Cutting research blocks from home? (Rene J Buesa) 5. Re: Salary Scales (Rene J Buesa) 6. Suggestions on Equipment?? (MICHELLE SEAGLE) 7. Re: Suggestions on Equipment?? (TBritten@aol.com) 8. cd38 on mouse tissue (Patsy Ruegg) 9. Re: Suggestions on Equipment?? (Rene J Buesa) 10. Sending privately Re: [Histonet] Glucose oxidase method for blocking endogenous peroxidase (Gayle Callis) 11. vibratome sections for cavalieri volume estimation (Claudia Lutz) 12. Re: ZYMED Histostain Plus Kit on animal tissue (Gayle Callis) 13. OCT vs. isopentane (shupeiwu@umich.edu) 14. Re: Standardized Microtomes (Amos Brooks) 15. RE: OCT vs. isopentane (Monfils, Paul) 16. Re: Standardized Microtomes (Gayle Callis) 17. Help identifying LS174T tumor cells (jstaruk) 18. Gamal Akabani has sent you a hi5 Friend Request (Gamal Akabani) 19. Re: Help identifying LS174T tumor cells (koellingr@comcast.net) 20. FW: [Histonet] Help identifying LS174T tumor cells (Jim Manavis) 21. Re: Suggestions on Equipment?? (Anne van Binsbergen) 22. Re: Standardized Microtomes (Rene J Buesa) 23. Looking for Melissa Barrett (Whitaker, Bonnie) 24. need knife guard (Rutledge, Nancy) 25. RE: Salary Scales (Dawson, Glen) 26. RE: Salary Scales (Rene J Buesa) 27. Alternative Preserving Medium for Histology (Rupert Street) ---------------------------------------------------------------------- Message: 1 Date: Wed, 14 May 2008 10:05:39 -0700 From: "Gale, Nadia" Subject: [Histonet] Glucose oxidase method for blocking endogenous peroxidase To: Message-ID: Content-Type: text/plain; charset="us-ascii" I've searched the archives and cannot find what I am after... I'd like to try the glucose oxidase method for blocking endogenous peroxidase in tissue sections for IHC. What is a suitable replacement for the B-d(+)glucose G5250 discontinued by Sigma? Will the D-(+)-Glucose G8270 work? This is 4% beta isomer and 96% alpha. Thanks for your help, Nadia Nadia Gale Lead Histotechnologist Centre for Translational and Applied Genomics (CTAG) 3427-600 West 10th Avenue Vancouver, BC V5Z 4E6 tel 604-877-6000 ext 2426 fax 604-877-6089 ngale@bccancer.bc.ca ------------------------------ Message: 2 Date: Wed, 14 May 2008 12:39:57 -0500 From: "Pam Bakken" Subject: [Histonet] Cutting research blocks from home? To: Message-ID: <482ADD9D.37F9.0000.0@childrensmn.org> Content-Type: text/plain; charset="us-ascii" Hi, I am interested in working part time from home cutting research blocks. Is there anyone that could give me some advice on how to get started? How do I charge? Any experience, advice or knowledge would be greatly appreciated. Thanks, Pamm Bakken Children's Hospital - Minneapolis _______________________________________________________________ Confidentiality Statement: This email/fax, including attachments, may include confidential and/or proprietary information and may be used only by the person or entity to which it is addressed. If the reader of this email/fax is not the intended recipient or his or her agent, the reader is hereby notified that any dissemination, distribution or copying of this email/fax is prohibited. If you have received this email/fax in error, please notify the sender by replying to this message and deleting this email or destroying this facsimile immediately. ------------------------------ Message: 3 Date: Wed, 14 May 2008 10:51:11 -0700 (PDT) From: Kim Merriam Subject: Re: [Histonet] ZYMED Histostain Plus Kit on animal tissue To: Mary L Giebel/FS/VCU , histonet@lists.utsouthwestern.edu Message-ID: <654253.27119.qm@web50308.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I dont have experience with this particular kit from Zymed, but the term "broad spectrum" makes me think that it?would be similar to a universal secondary (that might include anti-mouse and anti-rabbit secondaries) which would likely cross-react to your rat tissue.? It might be worth giving Zymed a call to find out about this kit. ?Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: Mary L Giebel/FS/VCU To: histonet@lists.utsouthwestern.edu Sent: Wednesday, May 14, 2008 12:56:51 PM Subject: [Histonet] ZYMED Histostain Plus Kit on animal tissue At a client's request, I am using a ZYMED's Broad Spectrum Histostain Plus Kit (#85-9843) on rat tissue. I have encountered an unexpected staining pattern as well as more background staining than usual. I would be very interested in hearing from others who have used this kit on animal tissue. I found the question posed in the Histonet archives (8/23/2005), but never found an answer posted. Thank you for your help. Regards, Mary Giebel ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Wed, 14 May 2008 12:18:02 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Cutting research blocks from home? To: Pam Bakken , histonet@lists.utsouthwestern.edu Message-ID: <734.60655.qm@web65716.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 After getting the "essentials" (microtome, water bath, oven, staining dishes and needed supplies) you have to decide a price. Charge by the slide. One H&E stain costs about $4.50, with everything else (from cutting to staining) I would charge $10/stained section. Ren? J. Pam Bakken wrote: ------------------------------ Message: 5 Date: Wed, 14 May 2008 12:21:40 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Salary Scales To: JR R , histonet@lists.utsouthwestern.edu Message-ID: <623891.86971.qm@web65712.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Which is equivalent to $26.20 / hour (2080 hours / year) and could be considered as "decent". Ren? J. JR R wrote: ------------------------------ Message: 6 Date: Wed, 14 May 2008 12:28:43 -0700 (PDT) From: MICHELLE SEAGLE Subject: [Histonet] Suggestions on Equipment?? To: histonet@lists.utsouthwestern.edu Message-ID: <739926.38110.qm@web51803.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Our hospital is currently looking to purchase a new cryostat, H& E Stainer and an Embedding Center. Any suggestions on a certain model,type or company with one that you have had a good experience with would be greatly appreciated. Michelle Seagle HT (ASCP) Rutherford Hospital MICHELLE SEAGLE ------------------------------ Message: 7 Date: Wed, 14 May 2008 15:36:57 EDT From: TBritten@aol.com Subject: Re: [Histonet] Suggestions on Equipment?? To: mrsseagle@yahoo.com, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" hello; we have used the "hacker" brand cryostat for many years at very high volumes and they have been very good. there are under a few brand marks but all who sell such things will be able to help you identify the latest model. tom In a message dated 5/14/2008 3:30:34 P.M. Eastern Daylight Time, mrsseagle@yahoo.com writes: Our hospital is currently looking to purchase a new cryostat, H& E Stainer and an Embedding Center. Any suggestions on a certain model,type or company with one that you have had a good experience with would be greatly appreciated. Michelle Seagle HT (ASCP) Rutherford Hospital MICHELLE SEAGLE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************Wondering what's for Dinner Tonight? Get new twists on family favorites at AOL Food. (http://food.aol.com/dinner-tonight?NCID=aolfod00030000000001) ------------------------------ Message: 8 Date: Wed, 14 May 2008 14:32:05 -0600 From: "Patsy Ruegg" Subject: [Histonet] cd38 on mouse tissue To: "'Histonet'" Message-ID: <003301c8b601$9378f720$6401a8c0@Patsyoffice> Content-Type: text/plain; charset="us-ascii" Anybody used cd38 on ffpe or frozen mouse tissue? Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. ------------------------------ Message: 9 Date: Wed, 14 May 2008 13:40:16 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Suggestions on Equipment?? To: MICHELLE SEAGLE , histonet@lists.utsouthwestern.edu Message-ID: <747728.50125.qm@web65703.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Cryostat from Leica; H&E stainer and embedding center from Sakura, although it is very likely that the Leica people (that manufacture the 3 pieces of equipment) will give you a better price if you buy all from them. Otherwise follow the first choice (Leica + Sakura). Ren? J. MICHELLE SEAGLE wrote: ------------------------------ Message: 10 Date: Wed, 14 May 2008 15:46:23 -0600 From: "Gayle Callis" Subject: Sending privately Re: [Histonet] Glucose oxidase method for blocking endogenous peroxidase To: "Gale, Nadia" , Message-ID: <000501c8b60b$f46ed9a0$6501a8c0@DHXTS541> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Nadia, I will attach the method and all the regeant needs to you privately and where you can purchase the correct glucose. No, glucose D-(=) Glucose will NOT work. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Gale, Nadia" To: Sent: Wednesday, May 14, 2008 11:05 AM Subject: [Histonet] Glucose oxidase method for blocking endogenous peroxidase I've searched the archives and cannot find what I am after... I'd like to try the glucose oxidase method for blocking endogenous peroxidase in tissue sections for IHC. What is a suitable replacement for the B-d(+)glucose G5250 discontinued by Sigma? Will the D-(+)-Glucose G8270 work? This is 4% beta isomer and 96% alpha. Thanks for your help, Nadia Nadia Gale Lead Histotechnologist Centre for Translational and Applied Genomics (CTAG) 3427-600 West 10th Avenue Vancouver, BC V5Z 4E6 tel 604-877-6000 ext 2426 fax 604-877-6089 ngale@bccancer.bc.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Wed, 14 May 2008 17:12:59 -0500 (CDT) From: "Claudia Lutz" Subject: [Histonet] vibratome sections for cavalieri volume estimation To: histonet@lists.utsouthwestern.edu Message-ID: <4769.130.126.50.22.1210803202.squirrel@www-s.life.uiuc.edu> Content-Type: text/plain;charset=iso-8859-1 Hi, I am fairly new to histological procedures and new to this list. My lab would like to stain vibratome sections of honey bee brain with phalloidin in order to estimate volume of specific brain regions. I am fixing the brains overnight in 4% paraformaldehyde, embedding in agarose, sectioning at 100 um, then staining free-floating sections in phalloidin with PBS with 0.2% Triton-X. I am mounting the slices in 80% glycerol and then imaging with the confocal microscope. I would like to use stereological procedures (specifically the cavalieri volume estimator) to quantify the volume of specific brain regions using systematic sampling of 10um optical sections that I obtain from the slices. I have several (possibly naive) questions: 1. My 100 um sections are shrinking to 60 um (or less) by the time I image them, judging by where I find or lose focus while imaging. What can I do to minimize this? How can I properly quantify the degree of shrinkage, to see whether or not it is uniform? Can I avoid bias in my volume estimation? 2. My phalloidin staining appears much brighter in the center of the slice, even when I use settings to adjust the gain while taking z-stacks. Does this indicate tissue damage at the edges? Can I ignore this? This also relates to my question above, since I am not sure I can precisely find the edge of the slice by focusing when I need to increase the gain. 3. I lose order and orientation of the slices because I am staining them free-floating. Does anyone have experience staining 40 or 50 um vibratome slices on the slide with phalloidin or antibody, or have a trustworthy protocol for this? Sorry to ask so many questions, and thank you for any help. There is not a lot of expertise in my lab on this, so I am feeling frustrated. Claudia Lutz University of Illinois at Urbana-Champaign Neuroscience Graduate Program ------------------------------ Message: 12 Date: Wed, 14 May 2008 16:22:26 -0600 From: "Gayle Callis" Subject: Re: [Histonet] ZYMED Histostain Plus Kit on animal tissue To: Message-ID: <003601c8b610$fd760000$6501a8c0@DHXTS541> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Kim, This kit detects (in its broad spectrum) - mouse, rabbit, rat and guinea pig. You are correct about this more universal, multilink secondary. The kit protocol is found on the Invitrogen website with information on what is in the secondary spectrum. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Kim Merriam" To: "Mary L Giebel/FS/VCU" ; Sent: Wednesday, May 14, 2008 11:51 AM Subject: Re: [Histonet] ZYMED Histostain Plus Kit on animal tissue I dont have experience with this particular kit from Zymed, but the term "broad spectrum" makes me think that it would be similar to a universal secondary (that might include anti-mouse and anti-rabbit secondaries) which would likely cross-react to your rat tissue. It might be worth giving Zymed a call to find out about this kit. Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: Mary L Giebel/FS/VCU To: histonet@lists.utsouthwestern.edu Sent: Wednesday, May 14, 2008 12:56:51 PM Subject: [Histonet] ZYMED Histostain Plus Kit on animal tissue At a client's request, I am using a ZYMED's Broad Spectrum Histostain Plus Kit (#85-9843) on rat tissue. I have encountered an unexpected staining pattern as well as more background staining than usual. I would be very interested in hearing from others who have used this kit on animal tissue. I found the question posed in the Histonet archives (8/23/2005), but never found an answer posted. Thank you for your help. Regards, Mary Giebel _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Wed, 14 May 2008 18:31:31 -0400 From: shupeiwu@umich.edu Subject: [Histonet] OCT vs. isopentane To: histonet@lists.utsouthwestern.edu Message-ID: <20080514183131.39132e3x7n46szfo@web.mail.umich.edu> Content-Type: text/plain; charset=ISO-8859-1; DelSp="Yes"; format="flowed" Hello all, I am quite new in the field of histochemistry. I'm doing mouse intestine cryostat section right now. I'm so confused about the method for cryostat section. First, I knew that most of time people using OCT to embed their sample, but I saw some papers they use liquid nitrogen cooled isopentane to embed their sample. Do these two methods cause any difference? Because I saw some background flurorescence in the OCT embedded slide. And if the sample embedded in isopentane, does the cryostat section take place in the same kind of machine? (with -20oC cryosection) Second, for the 4% Paraformaldehyde following by sucrose-PBS for cryoprotection, does it is really important to wash out the previous medium before entering the next step until the embedding. Third, does the thickness of the slide affect the autofluorescence? I knew those questions might some kind of vague. But thank you very much for any answers ahead. Wu ------------------------------ Message: 14 Date: Wed, 14 May 2008 18:42:54 -0400 From: "Amos Brooks" Subject: Re: [Histonet] Standardized Microtomes To: "Rene J Buesa" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <582736990805141542i42937995ub1411468c2feda9b@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Sorry Rene, I absolutly disagree with that. The exact opposite: *Not* modifying the angle of your block holder to suit the block, is not good practice. You are bound to loose tissue when cutting archived blocks, or blocks from other labs or even slight variations within your own lab. If you don't adapt to the blockyou are cutting. Without modifying the angle of the holder, one has 2 choices: Just cut thru it (refacing) or re-embedding and thereby needing to re-trim. Both scenerios will loose tissue. Often I have cut a freshly embedded block where the cassette didn't sit perfectly flat on the mold, otherwise the embedding was perfect. (Often due to poorly dissected tissue with high points). This results in an angle that is slightly askew. Adjusting the block holder is much faster than re-embedding the block. If it is done properly you can actually modify the block angle without ever cutting any tissue even on a previously cut block. We're going to have to disagree on this, Amos On Wed, May 14, 2008 at 9:14 AM, Rene J Buesa wrote: > All in all the practice of "changing the angle to match the block you > have to recut as needed" is not a good practice. > During it you could end loosing tissue when trying to "build" a new > flat surface from a block that was originally cut at a different > angle, while "sculping" the new surface. > The best practice is to have all the microtomes cutting at the same > angle and try to convince those HTs that say that "I have always cut > with this angle" that they are flat wrong and a general angle can be > good for the majority of blocks. > Ren? J. > ------------------------------ Message: 15 Date: Wed, 14 May 2008 18:57:17 -0400 From: "Monfils, Paul" Subject: RE: [Histonet] OCT vs. isopentane To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E273D7B@LSRIEXCH1.lsmaster.lifespan.org> Content-Type: text/plain; charset="iso-8859-1" Isopentane is not an embedding medium. OCT (or another comparable brand) is the embedding medium. Isopentane cooled with liquid nitrogen or dry ice is the freezing medium for freezing the liquid OCT into solid blocks. In sucrose cryoprotection, some people wash out the formalin before going into the first sucrose solution. I find that the sucrose solution itself washes out the formalin quite adequately. You do not wash at all between the different concentrations of sucrose. That would defeat the purpose of using an ascending series of concentrations. ------------------------------ Message: 16 Date: Wed, 14 May 2008 17:31:20 -0600 From: "Gayle Callis" Subject: Re: [Histonet] Standardized Microtomes To: "Amos Brooks" , "Rene J Buesa" Cc: histonet@lists.utsouthwestern.edu Message-ID: <006801c8b61a$9da832b0$6501a8c0@DHXTS541> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original When recutting a block or having one where the embedded tissue is a bit out whack for decent cutting alignment, then the x/y axis IS adjusted. The blade angle is generally not adjusted, only the x/y axis is changed. Even moving a blade sideways to access a sharp, new edge can alter the x/y orientation slightly. When this happens, reapproaching the blade, x/y adjustement may be needed to get first sections coming off the block - often the sections one absolutely must have. We don't change the blade angle unless someone tweaks the lever (generally an inexperienced tech or student who has no clue what is going on but they love flipping levers around). Sometimes a blade angle is readjusted from a new blade lot even though the manufacturer of the blade is the same. The blade manufacturing process can introduce minute changes in blades. When changing from high to low profile blades we readjust blade angle slightly at times - the low profiles being thinner in metal thickness and narrower width from top to bottom than the high profile. Rembedding and refacing a block is not ideal. As Amos points out, the danger of losing the region of interest in the embedded tissue is just too high especially on a recut. One can always turn a block in the holder, but that means some clever, careful x/y axis adjustment - we do this all the time. I vote for Amos's way as he just described it, and that is how we do it in our lab. We meaning "I" do it, the x/y axis way. The lever tweakers are generally gently repirmanded and taught the correct way for where a blade angle works best for the preferred blade in the lab. Gayle M. Callis HTL/HT/MT(ASCP) Bozemant MT ----- Original Message ----- From: "Amos Brooks" To: "Rene J Buesa" Cc: Sent: Wednesday, May 14, 2008 4:42 PM Subject: Re: [Histonet] Standardized Microtomes Sorry Rene, I absolutly disagree with that. The exact opposite: *Not* modifying the angle of your block holder to suit the block, is not good practice. You are bound to loose tissue when cutting archived blocks, or blocks from other labs or even slight variations within your own lab. If you don't adapt to the blockyou are cutting. Without modifying the angle of the holder, one has 2 choices: Just cut thru it (refacing) or re-embedding and thereby needing to re-trim. Both scenerios will loose tissue. Often I have cut a freshly embedded block where the cassette didn't sit perfectly flat on the mold, otherwise the embedding was perfect. (Often due to poorly dissected tissue with high points). This results in an angle that is slightly askew. Adjusting the block holder is much faster than re-embedding the block. If it is done properly you can actually modify the block angle without ever cutting any tissue even on a previously cut block. We're going to have to disagree on this, Amos On Wed, May 14, 2008 at 9:14 AM, Rene J Buesa wrote: > All in all the practice of "changing the angle to match the block you have > to recut as needed" is not a good practice. > During it you could end loosing tissue when trying to "build" a new flat > surface from a block that was originally cut at a different angle, while > "sculping" the new surface. > The best practice is to have all the microtomes cutting at the same angle > and try to convince those HTs that say that "I have always cut with this > angle" that they are flat wrong and a general angle can be good for the > majority of blocks. > Ren? J. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Wed, 14 May 2008 20:02:15 -0400 From: "jstaruk" Subject: [Histonet] Help identifying LS174T tumor cells To: Message-ID: Content-Type: text/plain; charset="US-ASCII" OK, I've been working on this for over a week and finally decided I need help. LS174T tumor cells (a human colon cancer cell line) were implanted into mouse lymph nodes. I'm trying to immuno-stain these implanted cells. I tried a pan cytokeratin antibody and a CK20 cytokeratin antibody and neither worked. Has anyone here ever worked with this cell line? Any suggestions on how to differentiate these cells from the normal mouse cells? Thanks! Jim _____________________ Jim Staruk Mass Histology Service www.masshistology.com ------------------------------ Message: 18 Date: Wed, 14 May 2008 18:09:26 -0700 From: Gamal Akabani Subject: [Histonet] Gamal Akabani has sent you a hi5 Friend Request To: histonet@lists.utsouthwestern.edu Message-ID: <1478199894.95.1210813766874.JavaMail.root@sfapp294> Content-Type: text/plain; charset="UTF-8" Gamal Akabani would like to be your friend on hi5! I set up a hi5 profile and I want to add you as a friend so we can share pictures and start building our network. First you need to join hi5! Once you join, you will have a chance to create a profile, share pictures, and find friends. Thanks, Gamal [1]Join hi5!? [2][nophoto_boy_100.gif] [3]Gamal Akabani 1 Friends ------------------------------------------------------ Copyright 2002-2008 Hi5 Networks, Inc. All rights reserved. P.O. Box 31118, San Francisco, CA 94131, USA [4]Privacy Policy | [5]Unsubscribe | [6]Terms of Service [to.do?loginid=&smid=] References 1. http://www.hi5.com/friend/registration/displayRegistration.do?inviteId=B_e6054e2_ZwJcpc6CJdh&smid= 2. http://www.hi5.com/friend/registration/displayRegistration.do?inviteId=B_e6054e2_ZwJcpc6CJdh&smid= 3. http://www.hi5.com/friend/registration/displayRegistration.do?inviteId=B_e6054e2_ZwJcpc6CJdh&smid= 4. http://www.hi5.com/friend/displayPrivacy.do 5. http://www.hi5.com/friend/account/displayEditPrivacy.do?loginid= 6. http://www.hi5.com/friend/displayTOS.do ------------------------------ Message: 19 Date: Thu, 15 May 2008 01:46:09 +0000 From: koellingr@comcast.net Subject: Re: [Histonet] Help identifying LS174T tumor cells To: "jstaruk" , Message-ID: <051520080146.9807.482B95E1000D52F40000264F22134843739D09020704040A0105@comcast.net> Content-Type: text/plain Jim, Haven't worked on that cell line in lymph nodes but in other parts of mice, xenografts and monolayers. I'm assuming these are nude mice so the cells aren't simply being destroyed by host (mouse) immune response. I'd stay away from cytokeratins. CK20 can be downregulated in that line and there are cytokeratins that endogenously mark in lymph nodes. That line I believe is presumed to be goblet cell associated. I've looked at it with CEA and villin, things other than CK20 or pan cytokeratin that might traditionally mark colon. Might try cdx2 besides CEA or villin. I believe in ATTC that line is known as a large producer of CEA and state of the art (back when I did this) was to localize LS174T's was radiolabelled CEA. Never tried but MUC antibody or even a PAS or mucin histology stain might do. Raymond Koelling PhenoPath Labs Seattle, WA -------------- Original message -------------- From: "jstaruk" > OK, I've been working on this for over a week and finally decided I need > help. LS174T tumor cells (a human colon cancer cell line) were implanted > into mouse lymph nodes. I'm trying to immuno-stain these implanted cells. > I tried a pan cytokeratin antibody and a CK20 cytokeratin antibody and > neither worked. Has anyone here ever worked with this cell line? Any > suggestions on how to differentiate these cells from the normal mouse cells? > > Thanks! > > Jim > > _____________________ > Jim Staruk > Mass Histology Service > www.masshistology.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Thu, 15 May 2008 12:44:08 +0930 From: "Jim Manavis" Subject: FW: [Histonet] Help identifying LS174T tumor cells To: "Histonet" Message-ID: <001c01c8b639$bd8796b0$636c140a@itp36533> Content-Type: text/plain; charset="US-ASCII" Jim I've gone through something like this a few years ago. We attempted to differentiate between host mouse cells and introduced human cancer cells. We used the human mitochondrial marker successfully on FFPE tissue to differentiate between the two. Initially we were using the Chemicon antibody (Cat No. MAB1273) but then struck a snag with it, where with a particular set of batch numbers it didn't work. We then turned to the Abcam antibody (Cat No. ab3298) and have been using this ever since Cheers Jim Jim Manavis Laboratory Manager Hanson Institute Centre for Neurological Diseases IMVS, Adelaide, SA, 5000 Australia Phone: 61-08-8222-3668 / 0401120697 FAX: 61-08-8222 3392 email: jim.manavis@imvs.sa.gov.au Disclaimer: Not this little black duck! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of koellingr@comcast.net Sent: Thursday, 15 May 2008 11:16 AM To: jstaruk; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Help identifying LS174T tumor cells Jim, Haven't worked on that cell line in lymph nodes but in other parts of mice, xenografts and monolayers. I'm assuming these are nude mice so the cells aren't simply being destroyed by host (mouse) immune response. I'd stay away from cytokeratins. CK20 can be downregulated in that line and there are cytokeratins that endogenously mark in lymph nodes. That line I believe is presumed to be goblet cell associated. I've looked at it with CEA and villin, things other than CK20 or pan cytokeratin that might traditionally mark colon. Might try cdx2 besides CEA or villin. I believe in ATTC that line is known as a large producer of CEA and state of the art (back when I did this) was to localize LS174T's was radiolabelled CEA. Never tried but MUC antibody or even a PAS or mucin histology stain might do. Raymond Koelling PhenoPath Labs Seattle, WA -------------- Original message -------------- From: "jstaruk" > OK, I've been working on this for over a week and finally decided I need > help. LS174T tumor cells (a human colon cancer cell line) were implanted > into mouse lymph nodes. I'm trying to immuno-stain these implanted cells. > I tried a pan cytokeratin antibody and a CK20 cytokeratin antibody and > neither worked. Has anyone here ever worked with this cell line? Any > suggestions on how to differentiate these cells from the normal mouse cells? > > Thanks! > > Jim > > _____________________ > Jim Staruk > Mass Histology Service > www.masshistology.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Thu, 15 May 2008 08:05:05 +0400 From: "Anne van Binsbergen" Subject: Re: [Histonet] Suggestions on Equipment?? To: "Rene J Buesa" Cc: histonet@lists.utsouthwestern.edu, MICHELLE SEAGLE Message-ID: Content-Type: text/plain; charset=ISO-8859-1 it seems like Rene and I were cut from a similar AP mould Leica Cryotat - go for the new one with the on board decontamination Sakura Embedder - its for left handers and right handers and the cold plate is a separate unit - neat and compact Sakura DRS2000 or the Prisma (with the attached coverslipper) - it has small staining dishes (optional) which i just love Sakura need to vamp up their Cryostat technology - then my whole lab will be a showcase of their products - are you reading this guys - LOL Annie 2008/5/15 Rene J Buesa : > Cryostat from Leica; H&E stainer and embedding center from Sakura, although > it is very likely that the Leica people (that manufacture the 3 pieces of > equipment) will give you a better price if you buy all from them. Otherwise > follow the first choice (Leica + Sakura). > Ren? J. > > MICHELLE SEAGLE wrote: > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE ------------------------------ Message: 22 Date: Thu, 15 May 2008 05:43:21 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Standardized Microtomes To: Amos Brooks Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <812281.7705.qm@web65711.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Amos: You had pointed out to the "acceptable exception". I do not advocate to NEVER align a blade to a block when necessary. What I say that for new block to be cut ALL microtomes in the lab should use the same angle and allow, for instance, that any HT could cut a recur for any special procedure without having to adjust the cutting angle to that specific block, because it was cut with an uniform angle. Archival blocks, or those received from another institution as a consult, of course that should be cut by adapting the cutting angle to the block, that is always better than reembedding the block. So, for NEW, everyday routine blocks: all microtomes with the same angle. For old or consult blocks: adapt the microtome to them. Besides, you don't have to be sorry to express your opinion! Ren? J. ------------------------------ Message: 23 Date: Thu, 15 May 2008 09:00:32 -0400 From: "Whitaker, Bonnie" Subject: [Histonet] Looking for Melissa Barrett To: histonet@lists.utsouthwestern.edu Message-ID: <3CE20ED86C4A114EBDF3BCE8DEFD8F60262493@msxc06.OSUMC.EDU> Content-Type: text/plain; charset=us-ascii Hi All, We are trying to locate Melissa Barrett (at one time she was at the South Bend Medical Foundation) regarding a revision and repackaging of an exercise she did for TechSample a few years back. Melissa, if you are here please email me with contact info, or if anyone else knows how to reach her, please let me know. Thanks, Bonnie Whitaker Histology Manager Ohio State University Medical Center 614.293.5048 ------------------------------ Message: 24 Date: Thu, 15 May 2008 10:33:45 -0400 From: "Rutledge, Nancy" Subject: [Histonet] need knife guard To: Message-ID: <47AD3B259E920D449F580E6AE82C2B8F210256@FHEXSVR2.FHDOMAIN1.capecodhealth.org> Content-Type: text/plain; charset="us-ascii" I'm looking for one of the old knife guards, metal clip, fits over blade that extends beyond knife holder. Happy to pay for it, only need one. Thanks Nancy Rutledge Falmouth Hospital 100 Ter Heun Drive Falmouth, MA 02540 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org ------------------------------ Message: 25 Date: Thu, 15 May 2008 09:59:48 -0500 From: "Dawson, Glen" Subject: RE: [Histonet] Salary Scales To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" $26.20 / hour is "decent"? For the Milwaukee area, that is a phenominal payrate. What regions see this as "run of the mill"? I wish I could offer techs this pay. It would certainly solve all of our staffing issues in one fell swoop. I also wonder where payscales like this are when the Histology pay scales are being compiled for publication. You look at our printed ranges and you cannot help but think that they are more useful to those who want to keep histo-pay low. Call me a coward but I don't feel comfortable quoting our average histotech pay. I'll just say that it is SIGNIFICANTLY below $26.20. Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Wednesday, May 14, 2008 2:22 PM To: JR R; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Salary Scales Which is equivalent to $26.20 / hour (2080 hours / year) and could be considered as "decent". Ren? J. JR R wrote: _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 26 Date: Thu, 15 May 2008 09:18:00 -0700 (PDT) From: Rene J Buesa Subject: RE: [Histonet] Salary Scales To: "Dawson, Glen" , histonet@lists.utsouthwestern.edu Message-ID: <355672.50007.qm@web65701.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Glen: And it will keep that way until histotechs star demanding what is deserved! Have you realized that histotechs are the only specialists in the medical lab that have to make decisions all along the process? When to reject a too thick slice of tissue to assure proper processing? What part to embed to cut? Up to where trim the block discarding parts of the specimen FOR EVER?! Which section to take or which to discard FOR EVER?! When to stop differentiation in a special stain? There is no other area of the ML that has to take so many decisions, and they are better paid. And will be until the HTs decide to take action and demand what is deserved. Just my opinion (as usual!). Ren? J. "Dawson, Glen" wrote: ------------------------------ Message: 27 Date: Thu, 15 May 2008 17:39:02 +0100 From: "Rupert Street" Subject: [Histonet] Alternative Preserving Medium for Histology To: histonet@lists.utsouthwestern.edu Message-ID: <2e02b98e0805150939y2eca73dcld8413d92c742e9d6@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Dear All; At the risk of running the gamut with your news group, and I have asked permission. Myself and my colleagues are looking for people interested in beta testing an alternative to formalin/formaldehyde for within the histopathology industry. Before you start cracking back with questions, this product is not a fixative so therefore is no use at all for long term preservation. It is purely focussed towards biopsy usage. The product has been adapted from a successful product used within the funeral industry which seeks to replace formalin based embalming wherever people have concerns etc. If you are interested in helping us please send your details to the attached e-mail. Regards Rupert Street ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 54, Issue 21 **************************************** 2007 Best Places to Work Finalist Visit our careers page at www.kaleidahealth.org/careers CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. 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From mpence <@t> grhs.net Fri May 16 10:27:06 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Fri May 16 10:27:14 2008 Subject: [Histonet] RE: Histonet Digest, Vol 54, Issue 21 In-Reply-To: <16A5E67B2A1F714885DEDC2CB68DD6090CB8EB@KALEXMB03.KaleidaHealth.org> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3840@IS-E2K3.grhs.net> I have not, as of yet, to see any delays or shortfalls of hematoxylin at this time in my orders. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Featherstone, Annette Sent: Friday, May 16, 2008 9:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 54, Issue 21 What is everyone doing about the hematoxylin shortage? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, May 15, 2008 12:48 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 54, Issue 21 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Glucose oxidase method for blocking endogenous peroxidase (Gale, Nadia) 2. Cutting research blocks from home? (Pam Bakken) 3. Re: ZYMED Histostain Plus Kit on animal tissue (Kim Merriam) 4. Re: Cutting research blocks from home? (Rene J Buesa) 5. Re: Salary Scales (Rene J Buesa) 6. Suggestions on Equipment?? (MICHELLE SEAGLE) 7. Re: Suggestions on Equipment?? (TBritten@aol.com) 8. cd38 on mouse tissue (Patsy Ruegg) 9. Re: Suggestions on Equipment?? (Rene J Buesa) 10. Sending privately Re: [Histonet] Glucose oxidase method for blocking endogenous peroxidase (Gayle Callis) 11. vibratome sections for cavalieri volume estimation (Claudia Lutz) 12. Re: ZYMED Histostain Plus Kit on animal tissue (Gayle Callis) 13. OCT vs. isopentane (shupeiwu@umich.edu) 14. Re: Standardized Microtomes (Amos Brooks) 15. RE: OCT vs. isopentane (Monfils, Paul) 16. Re: Standardized Microtomes (Gayle Callis) 17. Help identifying LS174T tumor cells (jstaruk) 18. Gamal Akabani has sent you a hi5 Friend Request (Gamal Akabani) 19. Re: Help identifying LS174T tumor cells (koellingr@comcast.net) 20. FW: [Histonet] Help identifying LS174T tumor cells (Jim Manavis) 21. Re: Suggestions on Equipment?? (Anne van Binsbergen) 22. Re: Standardized Microtomes (Rene J Buesa) 23. Looking for Melissa Barrett (Whitaker, Bonnie) 24. need knife guard (Rutledge, Nancy) 25. RE: Salary Scales (Dawson, Glen) 26. RE: Salary Scales (Rene J Buesa) 27. Alternative Preserving Medium for Histology (Rupert Street) ---------------------------------------------------------------------- Message: 1 Date: Wed, 14 May 2008 10:05:39 -0700 From: "Gale, Nadia" Subject: [Histonet] Glucose oxidase method for blocking endogenous peroxidase To: Message-ID: Content-Type: text/plain; charset="us-ascii" I've searched the archives and cannot find what I am after... I'd like to try the glucose oxidase method for blocking endogenous peroxidase in tissue sections for IHC. What is a suitable replacement for the B-d(+)glucose G5250 discontinued by Sigma? Will the D-(+)-Glucose G8270 work? This is 4% beta isomer and 96% alpha. Thanks for your help, Nadia Nadia Gale Lead Histotechnologist Centre for Translational and Applied Genomics (CTAG) 3427-600 West 10th Avenue Vancouver, BC V5Z 4E6 tel 604-877-6000 ext 2426 fax 604-877-6089 ngale@bccancer.bc.ca ------------------------------ Message: 2 Date: Wed, 14 May 2008 12:39:57 -0500 From: "Pam Bakken" Subject: [Histonet] Cutting research blocks from home? To: Message-ID: <482ADD9D.37F9.0000.0@childrensmn.org> Content-Type: text/plain; charset="us-ascii" Hi, I am interested in working part time from home cutting research blocks. Is there anyone that could give me some advice on how to get started? How do I charge? Any experience, advice or knowledge would be greatly appreciated. Thanks, Pamm Bakken Children's Hospital - Minneapolis _______________________________________________________________ Confidentiality Statement: This email/fax, including attachments, may include confidential and/or proprietary information and may be used only by the person or entity to which it is addressed. If the reader of this email/fax is not the intended recipient or his or her agent, the reader is hereby notified that any dissemination, distribution or copying of this email/fax is prohibited. If you have received this email/fax in error, please notify the sender by replying to this message and deleting this email or destroying this facsimile immediately. ------------------------------ Message: 3 Date: Wed, 14 May 2008 10:51:11 -0700 (PDT) From: Kim Merriam Subject: Re: [Histonet] ZYMED Histostain Plus Kit on animal tissue To: Mary L Giebel/FS/VCU , histonet@lists.utsouthwestern.edu Message-ID: <654253.27119.qm@web50308.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I dont have experience with this particular kit from Zymed, but the term "broad spectrum" makes me think that it?would be similar to a universal secondary (that might include anti-mouse and anti-rabbit secondaries) which would likely cross-react to your rat tissue.? It might be worth giving Zymed a call to find out about this kit. ?Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: Mary L Giebel/FS/VCU To: histonet@lists.utsouthwestern.edu Sent: Wednesday, May 14, 2008 12:56:51 PM Subject: [Histonet] ZYMED Histostain Plus Kit on animal tissue At a client's request, I am using a ZYMED's Broad Spectrum Histostain Plus Kit (#85-9843) on rat tissue. I have encountered an unexpected staining pattern as well as more background staining than usual. I would be very interested in hearing from others who have used this kit on animal tissue. I found the question posed in the Histonet archives (8/23/2005), but never found an answer posted. Thank you for your help. Regards, Mary Giebel ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Wed, 14 May 2008 12:18:02 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Cutting research blocks from home? To: Pam Bakken , histonet@lists.utsouthwestern.edu Message-ID: <734.60655.qm@web65716.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 After getting the "essentials" (microtome, water bath, oven, staining dishes and needed supplies) you have to decide a price. Charge by the slide. One H&E stain costs about $4.50, with everything else (from cutting to staining) I would charge $10/stained section. Ren? J. Pam Bakken wrote: ------------------------------ Message: 5 Date: Wed, 14 May 2008 12:21:40 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Salary Scales To: JR R , histonet@lists.utsouthwestern.edu Message-ID: <623891.86971.qm@web65712.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Which is equivalent to $26.20 / hour (2080 hours / year) and could be considered as "decent". Ren? J. JR R wrote: ------------------------------ Message: 6 Date: Wed, 14 May 2008 12:28:43 -0700 (PDT) From: MICHELLE SEAGLE Subject: [Histonet] Suggestions on Equipment?? To: histonet@lists.utsouthwestern.edu Message-ID: <739926.38110.qm@web51803.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Our hospital is currently looking to purchase a new cryostat, H& E Stainer and an Embedding Center. Any suggestions on a certain model,type or company with one that you have had a good experience with would be greatly appreciated. Michelle Seagle HT (ASCP) Rutherford Hospital MICHELLE SEAGLE ------------------------------ Message: 7 Date: Wed, 14 May 2008 15:36:57 EDT From: TBritten@aol.com Subject: Re: [Histonet] Suggestions on Equipment?? To: mrsseagle@yahoo.com, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" hello; we have used the "hacker" brand cryostat for many years at very high volumes and they have been very good. there are under a few brand marks but all who sell such things will be able to help you identify the latest model. tom In a message dated 5/14/2008 3:30:34 P.M. Eastern Daylight Time, mrsseagle@yahoo.com writes: Our hospital is currently looking to purchase a new cryostat, H& E Stainer and an Embedding Center. Any suggestions on a certain model,type or company with one that you have had a good experience with would be greatly appreciated. Michelle Seagle HT (ASCP) Rutherford Hospital MICHELLE SEAGLE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************Wondering what's for Dinner Tonight? Get new twists on family favorites at AOL Food. (http://food.aol.com/dinner-tonight?NCID=aolfod00030000000001) ------------------------------ Message: 8 Date: Wed, 14 May 2008 14:32:05 -0600 From: "Patsy Ruegg" Subject: [Histonet] cd38 on mouse tissue To: "'Histonet'" Message-ID: <003301c8b601$9378f720$6401a8c0@Patsyoffice> Content-Type: text/plain; charset="us-ascii" Anybody used cd38 on ffpe or frozen mouse tissue? Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. ------------------------------ Message: 9 Date: Wed, 14 May 2008 13:40:16 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Suggestions on Equipment?? To: MICHELLE SEAGLE , histonet@lists.utsouthwestern.edu Message-ID: <747728.50125.qm@web65703.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Cryostat from Leica; H&E stainer and embedding center from Sakura, although it is very likely that the Leica people (that manufacture the 3 pieces of equipment) will give you a better price if you buy all from them. Otherwise follow the first choice (Leica + Sakura). Ren? J. MICHELLE SEAGLE wrote: ------------------------------ Message: 10 Date: Wed, 14 May 2008 15:46:23 -0600 From: "Gayle Callis" Subject: Sending privately Re: [Histonet] Glucose oxidase method for blocking endogenous peroxidase To: "Gale, Nadia" , Message-ID: <000501c8b60b$f46ed9a0$6501a8c0@DHXTS541> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Nadia, I will attach the method and all the regeant needs to you privately and where you can purchase the correct glucose. No, glucose D-(=) Glucose will NOT work. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Gale, Nadia" To: Sent: Wednesday, May 14, 2008 11:05 AM Subject: [Histonet] Glucose oxidase method for blocking endogenous peroxidase I've searched the archives and cannot find what I am after... I'd like to try the glucose oxidase method for blocking endogenous peroxidase in tissue sections for IHC. What is a suitable replacement for the B-d(+)glucose G5250 discontinued by Sigma? Will the D-(+)-Glucose G8270 work? This is 4% beta isomer and 96% alpha. Thanks for your help, Nadia Nadia Gale Lead Histotechnologist Centre for Translational and Applied Genomics (CTAG) 3427-600 West 10th Avenue Vancouver, BC V5Z 4E6 tel 604-877-6000 ext 2426 fax 604-877-6089 ngale@bccancer.bc.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Wed, 14 May 2008 17:12:59 -0500 (CDT) From: "Claudia Lutz" Subject: [Histonet] vibratome sections for cavalieri volume estimation To: histonet@lists.utsouthwestern.edu Message-ID: <4769.130.126.50.22.1210803202.squirrel@www-s.life.uiuc.edu> Content-Type: text/plain;charset=iso-8859-1 Hi, I am fairly new to histological procedures and new to this list. My lab would like to stain vibratome sections of honey bee brain with phalloidin in order to estimate volume of specific brain regions. I am fixing the brains overnight in 4% paraformaldehyde, embedding in agarose, sectioning at 100 um, then staining free-floating sections in phalloidin with PBS with 0.2% Triton-X. I am mounting the slices in 80% glycerol and then imaging with the confocal microscope. I would like to use stereological procedures (specifically the cavalieri volume estimator) to quantify the volume of specific brain regions using systematic sampling of 10um optical sections that I obtain from the slices. I have several (possibly naive) questions: 1. My 100 um sections are shrinking to 60 um (or less) by the time I image them, judging by where I find or lose focus while imaging. What can I do to minimize this? How can I properly quantify the degree of shrinkage, to see whether or not it is uniform? Can I avoid bias in my volume estimation? 2. My phalloidin staining appears much brighter in the center of the slice, even when I use settings to adjust the gain while taking z-stacks. Does this indicate tissue damage at the edges? Can I ignore this? This also relates to my question above, since I am not sure I can precisely find the edge of the slice by focusing when I need to increase the gain. 3. I lose order and orientation of the slices because I am staining them free-floating. Does anyone have experience staining 40 or 50 um vibratome slices on the slide with phalloidin or antibody, or have a trustworthy protocol for this? Sorry to ask so many questions, and thank you for any help. There is not a lot of expertise in my lab on this, so I am feeling frustrated. Claudia Lutz University of Illinois at Urbana-Champaign Neuroscience Graduate Program ------------------------------ Message: 12 Date: Wed, 14 May 2008 16:22:26 -0600 From: "Gayle Callis" Subject: Re: [Histonet] ZYMED Histostain Plus Kit on animal tissue To: Message-ID: <003601c8b610$fd760000$6501a8c0@DHXTS541> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Kim, This kit detects (in its broad spectrum) - mouse, rabbit, rat and guinea pig. You are correct about this more universal, multilink secondary. The kit protocol is found on the Invitrogen website with information on what is in the secondary spectrum. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Kim Merriam" To: "Mary L Giebel/FS/VCU" ; Sent: Wednesday, May 14, 2008 11:51 AM Subject: Re: [Histonet] ZYMED Histostain Plus Kit on animal tissue I dont have experience with this particular kit from Zymed, but the term "broad spectrum" makes me think that it would be similar to a universal secondary (that might include anti-mouse and anti-rabbit secondaries) which would likely cross-react to your rat tissue. It might be worth giving Zymed a call to find out about this kit. Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: Mary L Giebel/FS/VCU To: histonet@lists.utsouthwestern.edu Sent: Wednesday, May 14, 2008 12:56:51 PM Subject: [Histonet] ZYMED Histostain Plus Kit on animal tissue At a client's request, I am using a ZYMED's Broad Spectrum Histostain Plus Kit (#85-9843) on rat tissue. I have encountered an unexpected staining pattern as well as more background staining than usual. I would be very interested in hearing from others who have used this kit on animal tissue. I found the question posed in the Histonet archives (8/23/2005), but never found an answer posted. Thank you for your help. Regards, Mary Giebel _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Wed, 14 May 2008 18:31:31 -0400 From: shupeiwu@umich.edu Subject: [Histonet] OCT vs. isopentane To: histonet@lists.utsouthwestern.edu Message-ID: <20080514183131.39132e3x7n46szfo@web.mail.umich.edu> Content-Type: text/plain; charset=ISO-8859-1; DelSp="Yes"; format="flowed" Hello all, I am quite new in the field of histochemistry. I'm doing mouse intestine cryostat section right now. I'm so confused about the method for cryostat section. First, I knew that most of time people using OCT to embed their sample, but I saw some papers they use liquid nitrogen cooled isopentane to embed their sample. Do these two methods cause any difference? Because I saw some background flurorescence in the OCT embedded slide. And if the sample embedded in isopentane, does the cryostat section take place in the same kind of machine? (with -20oC cryosection) Second, for the 4% Paraformaldehyde following by sucrose-PBS for cryoprotection, does it is really important to wash out the previous medium before entering the next step until the embedding. Third, does the thickness of the slide affect the autofluorescence? I knew those questions might some kind of vague. But thank you very much for any answers ahead. Wu ------------------------------ Message: 14 Date: Wed, 14 May 2008 18:42:54 -0400 From: "Amos Brooks" Subject: Re: [Histonet] Standardized Microtomes To: "Rene J Buesa" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <582736990805141542i42937995ub1411468c2feda9b@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Sorry Rene, I absolutly disagree with that. The exact opposite: *Not* modifying the angle of your block holder to suit the block, is not good practice. You are bound to loose tissue when cutting archived blocks, or blocks from other labs or even slight variations within your own lab. If you don't adapt to the blockyou are cutting. Without modifying the angle of the holder, one has 2 choices: Just cut thru it (refacing) or re-embedding and thereby needing to re-trim. Both scenerios will loose tissue. Often I have cut a freshly embedded block where the cassette didn't sit perfectly flat on the mold, otherwise the embedding was perfect. (Often due to poorly dissected tissue with high points). This results in an angle that is slightly askew. Adjusting the block holder is much faster than re-embedding the block. If it is done properly you can actually modify the block angle without ever cutting any tissue even on a previously cut block. We're going to have to disagree on this, Amos On Wed, May 14, 2008 at 9:14 AM, Rene J Buesa wrote: > All in all the practice of "changing the angle to match the block you > have to recut as needed" is not a good practice. > During it you could end loosing tissue when trying to "build" a new > flat surface from a block that was originally cut at a different > angle, while "sculping" the new surface. > The best practice is to have all the microtomes cutting at the same > angle and try to convince those HTs that say that "I have always cut > with this angle" that they are flat wrong and a general angle can be > good for the majority of blocks. > Ren? J. > ------------------------------ Message: 15 Date: Wed, 14 May 2008 18:57:17 -0400 From: "Monfils, Paul" Subject: RE: [Histonet] OCT vs. isopentane To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E273D7B@LSRIEXCH1.lsmaster.lifespan.org> Content-Type: text/plain; charset="iso-8859-1" Isopentane is not an embedding medium. OCT (or another comparable brand) is the embedding medium. Isopentane cooled with liquid nitrogen or dry ice is the freezing medium for freezing the liquid OCT into solid blocks. In sucrose cryoprotection, some people wash out the formalin before going into the first sucrose solution. I find that the sucrose solution itself washes out the formalin quite adequately. You do not wash at all between the different concentrations of sucrose. That would defeat the purpose of using an ascending series of concentrations. ------------------------------ Message: 16 Date: Wed, 14 May 2008 17:31:20 -0600 From: "Gayle Callis" Subject: Re: [Histonet] Standardized Microtomes To: "Amos Brooks" , "Rene J Buesa" Cc: histonet@lists.utsouthwestern.edu Message-ID: <006801c8b61a$9da832b0$6501a8c0@DHXTS541> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original When recutting a block or having one where the embedded tissue is a bit out whack for decent cutting alignment, then the x/y axis IS adjusted. The blade angle is generally not adjusted, only the x/y axis is changed. Even moving a blade sideways to access a sharp, new edge can alter the x/y orientation slightly. When this happens, reapproaching the blade, x/y adjustement may be needed to get first sections coming off the block - often the sections one absolutely must have. We don't change the blade angle unless someone tweaks the lever (generally an inexperienced tech or student who has no clue what is going on but they love flipping levers around). Sometimes a blade angle is readjusted from a new blade lot even though the manufacturer of the blade is the same. The blade manufacturing process can introduce minute changes in blades. When changing from high to low profile blades we readjust blade angle slightly at times - the low profiles being thinner in metal thickness and narrower width from top to bottom than the high profile. Rembedding and refacing a block is not ideal. As Amos points out, the danger of losing the region of interest in the embedded tissue is just too high especially on a recut. One can always turn a block in the holder, but that means some clever, careful x/y axis adjustment - we do this all the time. I vote for Amos's way as he just described it, and that is how we do it in our lab. We meaning "I" do it, the x/y axis way. The lever tweakers are generally gently repirmanded and taught the correct way for where a blade angle works best for the preferred blade in the lab. Gayle M. Callis HTL/HT/MT(ASCP) Bozemant MT ----- Original Message ----- From: "Amos Brooks" To: "Rene J Buesa" Cc: Sent: Wednesday, May 14, 2008 4:42 PM Subject: Re: [Histonet] Standardized Microtomes Sorry Rene, I absolutly disagree with that. The exact opposite: *Not* modifying the angle of your block holder to suit the block, is not good practice. You are bound to loose tissue when cutting archived blocks, or blocks from other labs or even slight variations within your own lab. If you don't adapt to the blockyou are cutting. Without modifying the angle of the holder, one has 2 choices: Just cut thru it (refacing) or re-embedding and thereby needing to re-trim. Both scenerios will loose tissue. Often I have cut a freshly embedded block where the cassette didn't sit perfectly flat on the mold, otherwise the embedding was perfect. (Often due to poorly dissected tissue with high points). This results in an angle that is slightly askew. Adjusting the block holder is much faster than re-embedding the block. If it is done properly you can actually modify the block angle without ever cutting any tissue even on a previously cut block. We're going to have to disagree on this, Amos On Wed, May 14, 2008 at 9:14 AM, Rene J Buesa wrote: > All in all the practice of "changing the angle to match the block you have > to recut as needed" is not a good practice. > During it you could end loosing tissue when trying to "build" a new flat > surface from a block that was originally cut at a different angle, while > "sculping" the new surface. > The best practice is to have all the microtomes cutting at the same angle > and try to convince those HTs that say that "I have always cut with this > angle" that they are flat wrong and a general angle can be good for the > majority of blocks. > Ren? J. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Wed, 14 May 2008 20:02:15 -0400 From: "jstaruk" Subject: [Histonet] Help identifying LS174T tumor cells To: Message-ID: Content-Type: text/plain; charset="US-ASCII" OK, I've been working on this for over a week and finally decided I need help. LS174T tumor cells (a human colon cancer cell line) were implanted into mouse lymph nodes. I'm trying to immuno-stain these implanted cells. I tried a pan cytokeratin antibody and a CK20 cytokeratin antibody and neither worked. Has anyone here ever worked with this cell line? Any suggestions on how to differentiate these cells from the normal mouse cells? Thanks! Jim _____________________ Jim Staruk Mass Histology Service www.masshistology.com ------------------------------ Message: 18 Date: Wed, 14 May 2008 18:09:26 -0700 From: Gamal Akabani Subject: [Histonet] Gamal Akabani has sent you a hi5 Friend Request To: histonet@lists.utsouthwestern.edu Message-ID: <1478199894.95.1210813766874.JavaMail.root@sfapp294> Content-Type: text/plain; charset="UTF-8" Gamal Akabani would like to be your friend on hi5! I set up a hi5 profile and I want to add you as a friend so we can share pictures and start building our network. First you need to join hi5! Once you join, you will have a chance to create a profile, share pictures, and find friends. Thanks, Gamal [1]Join hi5!? [2][nophoto_boy_100.gif] [3]Gamal Akabani 1 Friends ------------------------------------------------------ Copyright 2002-2008 Hi5 Networks, Inc. All rights reserved. P.O. Box 31118, San Francisco, CA 94131, USA [4]Privacy Policy | [5]Unsubscribe | [6]Terms of Service [to.do?loginid=&smid=] References 1. http://www.hi5.com/friend/registration/displayRegistration.do?inviteId=B_e6054e2_ZwJcpc6CJdh&smid= 2. http://www.hi5.com/friend/registration/displayRegistration.do?inviteId=B_e6054e2_ZwJcpc6CJdh&smid= 3. http://www.hi5.com/friend/registration/displayRegistration.do?inviteId=B_e6054e2_ZwJcpc6CJdh&smid= 4. http://www.hi5.com/friend/displayPrivacy.do 5. http://www.hi5.com/friend/account/displayEditPrivacy.do?loginid= 6. http://www.hi5.com/friend/displayTOS.do ------------------------------ Message: 19 Date: Thu, 15 May 2008 01:46:09 +0000 From: koellingr@comcast.net Subject: Re: [Histonet] Help identifying LS174T tumor cells To: "jstaruk" , Message-ID: <051520080146.9807.482B95E1000D52F40000264F22134843739D09020704040A0105@comcast.net> Content-Type: text/plain Jim, Haven't worked on that cell line in lymph nodes but in other parts of mice, xenografts and monolayers. I'm assuming these are nude mice so the cells aren't simply being destroyed by host (mouse) immune response. I'd stay away from cytokeratins. CK20 can be downregulated in that line and there are cytokeratins that endogenously mark in lymph nodes. That line I believe is presumed to be goblet cell associated. I've looked at it with CEA and villin, things other than CK20 or pan cytokeratin that might traditionally mark colon. Might try cdx2 besides CEA or villin. I believe in ATTC that line is known as a large producer of CEA and state of the art (back when I did this) was to localize LS174T's was radiolabelled CEA. Never tried but MUC antibody or even a PAS or mucin histology stain might do. Raymond Koelling PhenoPath Labs Seattle, WA -------------- Original message -------------- From: "jstaruk" > OK, I've been working on this for over a week and finally decided I need > help. LS174T tumor cells (a human colon cancer cell line) were implanted > into mouse lymph nodes. I'm trying to immuno-stain these implanted cells. > I tried a pan cytokeratin antibody and a CK20 cytokeratin antibody and > neither worked. Has anyone here ever worked with this cell line? Any > suggestions on how to differentiate these cells from the normal mouse cells? > > Thanks! > > Jim > > _____________________ > Jim Staruk > Mass Histology Service > www.masshistology.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Thu, 15 May 2008 12:44:08 +0930 From: "Jim Manavis" Subject: FW: [Histonet] Help identifying LS174T tumor cells To: "Histonet" Message-ID: <001c01c8b639$bd8796b0$636c140a@itp36533> Content-Type: text/plain; charset="US-ASCII" Jim I've gone through something like this a few years ago. We attempted to differentiate between host mouse cells and introduced human cancer cells. We used the human mitochondrial marker successfully on FFPE tissue to differentiate between the two. Initially we were using the Chemicon antibody (Cat No. MAB1273) but then struck a snag with it, where with a particular set of batch numbers it didn't work. We then turned to the Abcam antibody (Cat No. ab3298) and have been using this ever since Cheers Jim Jim Manavis Laboratory Manager Hanson Institute Centre for Neurological Diseases IMVS, Adelaide, SA, 5000 Australia Phone: 61-08-8222-3668 / 0401120697 FAX: 61-08-8222 3392 email: jim.manavis@imvs.sa.gov.au Disclaimer: Not this little black duck! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of koellingr@comcast.net Sent: Thursday, 15 May 2008 11:16 AM To: jstaruk; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Help identifying LS174T tumor cells Jim, Haven't worked on that cell line in lymph nodes but in other parts of mice, xenografts and monolayers. I'm assuming these are nude mice so the cells aren't simply being destroyed by host (mouse) immune response. I'd stay away from cytokeratins. CK20 can be downregulated in that line and there are cytokeratins that endogenously mark in lymph nodes. That line I believe is presumed to be goblet cell associated. I've looked at it with CEA and villin, things other than CK20 or pan cytokeratin that might traditionally mark colon. Might try cdx2 besides CEA or villin. I believe in ATTC that line is known as a large producer of CEA and state of the art (back when I did this) was to localize LS174T's was radiolabelled CEA. Never tried but MUC antibody or even a PAS or mucin histology stain might do. Raymond Koelling PhenoPath Labs Seattle, WA -------------- Original message -------------- From: "jstaruk" > OK, I've been working on this for over a week and finally decided I need > help. LS174T tumor cells (a human colon cancer cell line) were implanted > into mouse lymph nodes. I'm trying to immuno-stain these implanted cells. > I tried a pan cytokeratin antibody and a CK20 cytokeratin antibody and > neither worked. Has anyone here ever worked with this cell line? Any > suggestions on how to differentiate these cells from the normal mouse cells? > > Thanks! > > Jim > > _____________________ > Jim Staruk > Mass Histology Service > www.masshistology.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Thu, 15 May 2008 08:05:05 +0400 From: "Anne van Binsbergen" Subject: Re: [Histonet] Suggestions on Equipment?? To: "Rene J Buesa" Cc: histonet@lists.utsouthwestern.edu, MICHELLE SEAGLE Message-ID: Content-Type: text/plain; charset=ISO-8859-1 it seems like Rene and I were cut from a similar AP mould Leica Cryotat - go for the new one with the on board decontamination Sakura Embedder - its for left handers and right handers and the cold plate is a separate unit - neat and compact Sakura DRS2000 or the Prisma (with the attached coverslipper) - it has small staining dishes (optional) which i just love Sakura need to vamp up their Cryostat technology - then my whole lab will be a showcase of their products - are you reading this guys - LOL Annie 2008/5/15 Rene J Buesa : > Cryostat from Leica; H&E stainer and embedding center from Sakura, although > it is very likely that the Leica people (that manufacture the 3 pieces of > equipment) will give you a better price if you buy all from them. Otherwise > follow the first choice (Leica + Sakura). > Ren? J. > > MICHELLE SEAGLE wrote: > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE ------------------------------ Message: 22 Date: Thu, 15 May 2008 05:43:21 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Standardized Microtomes To: Amos Brooks Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <812281.7705.qm@web65711.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Amos: You had pointed out to the "acceptable exception". I do not advocate to NEVER align a blade to a block when necessary. What I say that for new block to be cut ALL microtomes in the lab should use the same angle and allow, for instance, that any HT could cut a recur for any special procedure without having to adjust the cutting angle to that specific block, because it was cut with an uniform angle. Archival blocks, or those received from another institution as a consult, of course that should be cut by adapting the cutting angle to the block, that is always better than reembedding the block. So, for NEW, everyday routine blocks: all microtomes with the same angle. For old or consult blocks: adapt the microtome to them. Besides, you don't have to be sorry to express your opinion! Ren? J. ------------------------------ Message: 23 Date: Thu, 15 May 2008 09:00:32 -0400 From: "Whitaker, Bonnie" Subject: [Histonet] Looking for Melissa Barrett To: histonet@lists.utsouthwestern.edu Message-ID: <3CE20ED86C4A114EBDF3BCE8DEFD8F60262493@msxc06.OSUMC.EDU> Content-Type: text/plain; charset=us-ascii Hi All, We are trying to locate Melissa Barrett (at one time she was at the South Bend Medical Foundation) regarding a revision and repackaging of an exercise she did for TechSample a few years back. Melissa, if you are here please email me with contact info, or if anyone else knows how to reach her, please let me know. Thanks, Bonnie Whitaker Histology Manager Ohio State University Medical Center 614.293.5048 ------------------------------ Message: 24 Date: Thu, 15 May 2008 10:33:45 -0400 From: "Rutledge, Nancy" Subject: [Histonet] need knife guard To: Message-ID: <47AD3B259E920D449F580E6AE82C2B8F210256@FHEXSVR2.FHDOMAIN1.capecodhealth.org> Content-Type: text/plain; charset="us-ascii" I'm looking for one of the old knife guards, metal clip, fits over blade that extends beyond knife holder. Happy to pay for it, only need one. Thanks Nancy Rutledge Falmouth Hospital 100 Ter Heun Drive Falmouth, MA 02540 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org ------------------------------ Message: 25 Date: Thu, 15 May 2008 09:59:48 -0500 From: "Dawson, Glen" Subject: RE: [Histonet] Salary Scales To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" $26.20 / hour is "decent"? For the Milwaukee area, that is a phenominal payrate. What regions see this as "run of the mill"? I wish I could offer techs this pay. It would certainly solve all of our staffing issues in one fell swoop. I also wonder where payscales like this are when the Histology pay scales are being compiled for publication. You look at our printed ranges and you cannot help but think that they are more useful to those who want to keep histo-pay low. Call me a coward but I don't feel comfortable quoting our average histotech pay. I'll just say that it is SIGNIFICANTLY below $26.20. Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Wednesday, May 14, 2008 2:22 PM To: JR R; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Salary Scales Which is equivalent to $26.20 / hour (2080 hours / year) and could be considered as "decent". Ren? J. JR R wrote: _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 26 Date: Thu, 15 May 2008 09:18:00 -0700 (PDT) From: Rene J Buesa Subject: RE: [Histonet] Salary Scales To: "Dawson, Glen" , histonet@lists.utsouthwestern.edu Message-ID: <355672.50007.qm@web65701.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Glen: And it will keep that way until histotechs star demanding what is deserved! Have you realized that histotechs are the only specialists in the medical lab that have to make decisions all along the process? When to reject a too thick slice of tissue to assure proper processing? What part to embed to cut? Up to where trim the block discarding parts of the specimen FOR EVER?! Which section to take or which to discard FOR EVER?! When to stop differentiation in a special stain? There is no other area of the ML that has to take so many decisions, and they are better paid. And will be until the HTs decide to take action and demand what is deserved. Just my opinion (as usual!). Ren? J. "Dawson, Glen" wrote: ------------------------------ Message: 27 Date: Thu, 15 May 2008 17:39:02 +0100 From: "Rupert Street" Subject: [Histonet] Alternative Preserving Medium for Histology To: histonet@lists.utsouthwestern.edu Message-ID: <2e02b98e0805150939y2eca73dcld8413d92c742e9d6@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Dear All; At the risk of running the gamut with your news group, and I have asked permission. Myself and my colleagues are looking for people interested in beta testing an alternative to formalin/formaldehyde for within the histopathology industry. Before you start cracking back with questions, this product is not a fixative so therefore is no use at all for long term preservation. It is purely focussed towards biopsy usage. The product has been adapted from a successful product used within the funeral industry which seeks to replace formalin based embalming wherever people have concerns etc. If you are interested in helping us please send your details to the attached e-mail. Regards Rupert Street ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 54, Issue 21 **************************************** 2007 Best Places to Work Finalist Visit our careers page at www.kaleidahealth.org/careers CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TBritten <@t> aol.com Fri May 16 10:34:55 2008 From: TBritten <@t> aol.com (TBritten@aol.com) Date: Fri May 16 10:35:24 2008 Subject: [Histonet] Cutting research blocks from home? Message-ID: i would be very cautious in starting this program...numerous liabilities could follow, the tracking of these materials, are you zoned at your home for such things, as mentioned bsl-2 or even bsl-3 required environment, disposal of material (medical waste), etc etc. i'm in my own business and only wish you the best but we do live in a very litigious society..tom In a message dated 5/15/2008 5:57:23 P.M. Eastern Daylight Time, talulahgosh@gmail.com writes: On Thu, May 15, 2008 at 12:58 PM, Kimberly Tuttle wrote: > Is there anyone on the list who does this? How is a "basement lab" regulated? If you dont do any staining and only cut unstained, therefore no reagents, is there no need to be regulated? > I would look into the institutions from who(m?) you're getting the specimens. They might not let anyone take the tissue off of the facility grounds. With a university lab, we can't even move tissue to another lab without filling out forms. Also, human tissue is BSL-2, at least, right? Emily -- If trees could scream, would we be so cavalier about cutting them down? We might, if they screamed all the time, for no good reason. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************Wondering what's for Dinner Tonight? Get new twists on family favorites at AOL Food. (http://food.aol.com/dinner-tonight?NCID=aolfod00030000000001) From rjbuesa <@t> yahoo.com Fri May 16 10:41:25 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri May 16 10:41:32 2008 Subject: [Histonet] Celestine Blue Procedure In-Reply-To: Message-ID: <394513.24571.qm@web65703.mail.ac4.yahoo.com> Ramona: The trick with celestine blue is the formula you use to prepare the staining solution. Gray prefers (and I have also used years ago) one that is as follows: water -- 100mL + ferric alum -- 2.5g + celestin blue B-- 0.5g + glycerol -- 14 mL + sulfuric acid -- 2 mL. Boil the dye in the alum solution (prepared first). Cool, filter and add the rest. Staining from 5 min to 1 hour (too long for a routine lab); will not overstain. Does not need "bluing". I don't think the hematoxylin will be unavailable any time soon. Ren? J. Ramona Turner wrote: From kenneth.metzger <@t> aruplab.com Fri May 16 11:00:40 2008 From: kenneth.metzger <@t> aruplab.com (Metzger, Kenneth) Date: Fri May 16 11:00:03 2008 Subject: [Histonet] Keratin-903 on Liver Message-ID: One of our pathologists wants us to use K-903 on liver to tag the bile duct. Though our procedure works great on prostate we are having trouble with it on the liver..any suggestions? Thanks Ken Metzger HTL(ASCP) Histology Supervisor ARUP Laboratories 500 Chipeta way Salt Lake City, UT 84108 801.583.2787 ext 3101 - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From dgaupp <@t> tulane.edu Fri May 16 11:04:42 2008 From: dgaupp <@t> tulane.edu (Gaupp, Dina D ) Date: Fri May 16 11:36:24 2008 Subject: [Histonet] fresh-frozen mouse tail sectioning Message-ID: <447056A67472B241A330A525B4AF71672061EF@EX02.ad.tulane.edu> To Whomever This Applies: I am having big problems sectioning mouse tail, approximately 2mm in thickness, vertical embedded in OCT for frozen sections. The tissue is fresh-frozen because the principle investigator would like to detect an enzyme x-gal. No fix, no cryopreservation - the tissue was not immersed in any type of solution. He snipped the ends of a mouse tail & immediately gave me the tissues. I embedded the tissue vertically in OCT & flash froze at -80C. Upon sectioning, the tissue rolled. I could not for the life of me get 1 section. All the tissues rolled & its so tiny to begin with that it was hard for me to grab it. I used the anti-roll plate, hoping it would hold the tissue in place but it still rolled. I changed temperature settings(increase & decrease temp), thickness, angle, rubbed it with my fingers. Everything I could think of to get a section. I asked someone else in the lab to try & they couldn't get a section. It was like the tissue completely separated from OCT. The principle investigator will give me more samples & I don't want this to happen again. Can anyone in histoland help me, tell me what I didn't do or what I did wrong? Dina D. Gaupp, BS, MT Senior Lab Supervisor Center for Gene Therapy, SL-99 Tulane University Health Science Center 1430 Tulane Ave New Orleans, La 70112 Lab: 504-988-1194 dgaupp@tulane.edu From rjbuesa <@t> yahoo.com Fri May 16 11:48:02 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri May 16 11:48:06 2008 Subject: [Histonet] fresh-frozen mouse tail sectioning In-Reply-To: <447056A67472B241A330A525B4AF71672061EF@EX02.ad.tulane.edu> Message-ID: <286758.49135.qm@web65716.mail.ac4.yahoo.com> I would try the investigator to give me the tail tip BEFORE freezing it, and include it in the OCT and freeze it then. I think that being frozen it is more difficult to be held in place by the OCT. I would also try to use transfer film for the sections. Ren? J. "Gaupp, Dina D " wrote: http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Fri May 16 11:53:40 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri May 16 11:53:41 2008 Subject: [Histonet] fresh-frozen mouse tail sectioning In-Reply-To: <447056A67472B241A330A525B4AF71672061EF@EX02.ad.tulane.edu> References: <447056A67472B241A330A525B4AF71672061EF@EX02.ad.tulane.edu> Message-ID: <010101c8b775$659cf160$6401a8c0@Patsyoffice> I would just lay it on it's side for freezing the first time and then turn it vertically to mount onto a chuck for sectioning. Also, you probably do not have this, but for difficult things like this (the tail will have at least cartilage in it) I use the tape transfer technique from Instrumedics (now owned by McCormick). Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gaupp, Dina D Sent: Friday, May 16, 2008 10:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fresh-frozen mouse tail sectioning To Whomever This Applies: I am having big problems sectioning mouse tail, approximately 2mm in thickness, vertical embedded in OCT for frozen sections. The tissue is fresh-frozen because the principle investigator would like to detect an enzyme x-gal. No fix, no cryopreservation - the tissue was not immersed in any type of solution. He snipped the ends of a mouse tail & immediately gave me the tissues. I embedded the tissue vertically in OCT & flash froze at -80C. Upon sectioning, the tissue rolled. I could not for the life of me get 1 section. All the tissues rolled & its so tiny to begin with that it was hard for me to grab it. I used the anti-roll plate, hoping it would hold the tissue in place but it still rolled. I changed temperature settings(increase & decrease temp), thickness, angle, rubbed it with my fingers. Everything I could think of to get a section. I asked someone else in the lab to try & they couldn't get a section. It was like the tissue completely separated from OCT. The principle investigator will give me more samples & I don't want this to happen again. Can anyone in histoland help me, tell me what I didn't do or what I did wrong? Dina D. Gaupp, BS, MT Senior Lab Supervisor Center for Gene Therapy, SL-99 Tulane University Health Science Center 1430 Tulane Ave New Orleans, La 70112 Lab: 504-988-1194 dgaupp@tulane.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ploykasek <@t> phenopath.com Fri May 16 12:22:21 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri May 16 12:22:31 2008 Subject: [Histonet] Keratin-903 on Liver In-Reply-To: Message-ID: Hi Ken. By referring to K-903, I believe you mean cytokeratin clone 34BE12. The CK-903 designation is actually an old catalog number, not the name of the keratin. Dr. Allen Gown made the 34BE12 antibody about 20 years ago. It's a high molecular weight keratin (CK1,5,10 & 14). Just a bit of history for you. But on to helping with your staining issues. This keratin should stain the bile ducts in liver. What pretreatment are you using? This antibody usually works with either an enzyme digestion or a gentle heat retrieval in citrate. Let me know if you need more info. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > One of our pathologists wants us to use K-903 on liver to tag the bile duct. > Though our procedure works great on prostate we are having trouble with it on > the liver..any suggestions? Thanks > > Ken Metzger HTL(ASCP) > Histology Supervisor > ARUP Laboratories > 500 Chipeta way > Salt Lake City, UT 84108 > 801.583.2787 ext 3101 > > > - ------------------------------------------------------------------ > The information transmitted by this e-mail and any included > attachments are from ARUP Laboratories and are intended only for the > recipient. The information contained in this message is confidential > and may constitute inside or non-public information under > international, federal, or state securities laws, or protected health > information and is intended only for the use of the recipient. > Unauthorized forwarding, printing, copying, distributing, or use of > such information is strictly prohibited and may be unlawful. If you > are not the intended recipient, please promptly delete this e-mail > and notify the sender of the delivery error or you may call ARUP > Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 > (800) 522-2787 ext. 2100 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From Rcartun <@t> harthosp.org Fri May 16 12:22:35 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri May 16 12:22:49 2008 Subject: [Histonet] AEC chromogen - coverslipping Message-ID: <482D8A9B020000770000C9EC@gwmail4.harthosp.org> If you use AEC as a chromogen for IHC staining, what are using to protect it before putting the slides into xylol for permanent coverslipping? Thanks! Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From christiegowan <@t> msn.com Fri May 16 12:24:32 2008 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Fri May 16 12:24:39 2008 Subject: [Histonet] Sakura tape coverslip problem In-Reply-To: <010101c8b775$659cf160$6401a8c0@Patsyoffice> Message-ID: Does anyone have a recovery method for tape coverslips that become detatched from the slide taking the tissue with it? I have tried several things but have not found a really good way to do this. I called the company and they did not have a procedure. Thanks, Christie Gowan UAB Hospital Birmingham, AL From rachelr <@t> nei.nih.gov Fri May 16 12:56:06 2008 From: rachelr <@t> nei.nih.gov (Rachel, Rivka (NIH/NEI) [E]) Date: Fri May 16 12:57:54 2008 Subject: [Histonet] fresh-frozen mouse tail sectioning References: <447056A67472B241A330A525B4AF71672061EF@EX02.ad.tulane.edu> Message-ID: Do you have to freeze or section the tissue at all? If there is significant beta-galactosidase activity, you should be able to just put the tail tip in X-gal and see if it turns blue, having of course appropriate positive and negative control tails that are known to carry or not carry, respectively, the relevant transgene. Rivka -- Rivka A. Rachel, MD, PhD Staff Scientist, National Eye Institute Neurobiology-Neurodegeneration and Repair Laboratory Tel: 301 443-4906 -----Original Message----- From: Gaupp, Dina D [mailto:dgaupp@tulane.edu] Sent: Fri 5/16/2008 12:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fresh-frozen mouse tail sectioning To Whomever This Applies: I am having big problems sectioning mouse tail, approximately 2mm in thickness, vertical embedded in OCT for frozen sections. The tissue is fresh-frozen because the principle investigator would like to detect an enzyme x-gal. No fix, no cryopreservation - the tissue was not immersed in any type of solution. He snipped the ends of a mouse tail & immediately gave me the tissues. I embedded the tissue vertically in OCT & flash froze at -80C. Upon sectioning, the tissue rolled. I could not for the life of me get 1 section. All the tissues rolled & its so tiny to begin with that it was hard for me to grab it. I used the anti-roll plate, hoping it would hold the tissue in place but it still rolled. I changed temperature settings(increase & decrease temp), thickness, angle, rubbed it with my fingers. Everything I could think of to get a section. I asked someone else in the lab to try & they couldn't get a section. It was like the tissue completely separated from OCT. The principle investigator will give me more samples & I don't want this to happen again. Can anyone in histoland help me, tell me what I didn't do or what I did wrong? Dina D. Gaupp, BS, MT Senior Lab Supervisor Center for Gene Therapy, SL-99 Tulane University Health Science Center 1430 Tulane Ave New Orleans, La 70112 Lab: 504-988-1194 dgaupp@tulane.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lloyd.3 <@t> osu.edu Fri May 16 13:04:41 2008 From: lloyd.3 <@t> osu.edu (Mary Lloyd) Date: Fri May 16 13:04:47 2008 Subject: [Histonet] Calcium oxylate stain Message-ID: <2096B6FC591D034FA50AFFD7A42EE96506774F61@dental.dentnet.dent.ohio-state.edu> My oral pathologist is requesting a specific stain for calcium oxylate. I have done von kossa for calcium but he wants something more specific. I would appreciate any help. Thanks Mary From dcrippen <@t> buckinstitute.org Fri May 16 13:07:51 2008 From: dcrippen <@t> buckinstitute.org (Danielle Crippen) Date: Fri May 16 13:07:56 2008 Subject: [Histonet] unsubscribe Message-ID: Danielle Crippen Morphology and Imaging Core x2046 From tissuetech <@t> juno.com Fri May 16 13:28:10 2008 From: tissuetech <@t> juno.com (tissuetech@juno.com) Date: Fri May 16 13:29:46 2008 Subject: [Histonet] Sakura tape coverslip problem Message-ID: <20080516.132810.15454.0@webmail14.vgs.untd.com> Christie Sorry about your problem. At this time there is nothing that I know of where you can remove the tissue from the tape and recover slip. I know you are caught between a rock & a hard place, but over the years no one that I know has ever been able to retrieve and remount. Sorry I can't be of more help. Fred S - Tissue Techniques Path Labs _____________________________________________________________ Click here to save cash and find low rates on auto loans. http://thirdpartyoffers.juno.com/TGL2121/fc/Ioyw6i3ndyH35dbInkZRPhRa58lNrdsPTYWRhADbbiHInlQ8K8YNeR/?count=1234567890 From tjasper <@t> copc.net Fri May 16 13:41:27 2008 From: tjasper <@t> copc.net (Thomas Jasper) Date: Fri May 16 13:41:34 2008 Subject: [Histonet] Calcium oxylate stain References: <2096B6FC591D034FA50AFFD7A42EE96506774F61@dental.dentnet.dent.ohio-state.edu> Message-ID: <90354A475B420441B2A0396E5008D4965E20BF@copc-sbs.COPC.local> How about Alizarin Red S? Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mary Lloyd Sent: Friday, May 16, 2008 11:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Calcium oxylate stain My oral pathologist is requesting a specific stain for calcium oxylate. I have done von kossa for calcium but he wants something more specific. I would appreciate any help. Thanks Mary _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Fri May 16 14:10:02 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Fri May 16 14:10:05 2008 Subject: [Histonet] Calcium oxylate stain References: <2096B6FC591D034FA50AFFD7A42EE96506774F61@dental.dentnet.dent.ohio-state.edu> Message-ID: <006401c8b788$71b60a50$6501a8c0@DHXTS541> Pizzolata's stain for calcium oxalate. I has been discussed on Histonet in the past, and you may be able to pick up the method on the web. Sheehan and Hrapchak Theory and Practice of Histotechnology, second edition has the method. Very easy to do. We ran the von Kossa on an adjacent section to distinguish between calcium oxalate and other calcium salt deposits, and eliminate one or the other for diagnostic purposes. A good calcium oxalate positive control is a dog or cat kidney that drank antifreeze containing ethylene glycol. The crystals are a bit refractile looking in an H&E section. A local veterinary diagnositic laboratory may have tissues available. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Mary Lloyd" To: Sent: Friday, May 16, 2008 12:04 PM Subject: [Histonet] Calcium oxylate stain My oral pathologist is requesting a specific stain for calcium oxylate. I have done von kossa for calcium but he wants something more specific. I would appreciate any help. Thanks Mary _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Fri May 16 14:12:53 2008 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Fri May 16 14:12:56 2008 Subject: [Histonet] AEC Chromagen - Coverslipping Message-ID: <150685.15173.qm@web50109.mail.re2.yahoo.com> I use Crystal Mount. After rinsing to blue the hematoxylin,?place a drop to cover the section, drain excess, let dry completely, and coverslip with resin mounting media. ?Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 631 N. Broadway Ave. Moore, OK 73160 405-570-6679 cell 405-759-3953 lab contact@excaliburpathology.com www.excaliburpathology.com From gayle.callis <@t> bresnan.net Fri May 16 14:18:31 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Fri May 16 14:18:32 2008 Subject: [Histonet] AEC chromogen - coverslipping References: <482D8A9B020000770000C9EC@gwmail4.harthosp.org> Message-ID: <006e01c8b789$a0f962c0$6501a8c0@DHXTS541> Richard, We have used the liquid mounting media, Crystal Mount from Biomeda - ThermoFisher used to carry this. Do the liquid coverslip first, let it dry according to directions i.e. several ways with heat, then mount a permanent coverglass over the top of this. AEC is preserved. If you try to look at the section before mounting a permanent coverslip, it can look a bit out of focus. Make sure the media covers the section smoothly and as flat at possible to avoid the "waves" of media. Someone recently posted the contact for Biomeda on Histonet on the chance ThermoFisher is not selling Crystal Mount anymore. There are other companies that supply this type of mounting media but not sure which vendors. Good luck Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Richard Cartun" To: "Histonet" Sent: Friday, May 16, 2008 11:22 AM Subject: [Histonet] AEC chromogen - coverslipping If you use AEC as a chromogen for IHC staining, what are using to protect it before putting the slides into xylol for permanent coverslipping? Thanks! Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Fri May 16 14:40:16 2008 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Fri May 16 14:40:21 2008 Subject: [Histonet] looking for c-kit In-Reply-To: <90354A475B420441B2A0396E5008D4965E20BF@copc-sbs.COPC.local> References: <2096B6FC591D034FA50AFFD7A42EE96506774F61@dental.dentnet.dent.ohi o-state.edu> <90354A475B420441B2A0396E5008D4965E20BF@copc-sbs.COPC.local> Message-ID: <541B786D4309163175E035B6@bchwxp2702.ad.med.buffalo.edu> I'm looking for an indirect immunofluorescence procedure for localizing c-kit on formaldehyde-fixed, paraffin-embedded tissue. What type of AR do you use, and do you permeabilize? Thanks, Merced M Leiker Research Technician II 354 BRB (Lee Lab) / 140 Farber Hall (mail) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 From rjbuesa <@t> yahoo.com Fri May 16 15:03:50 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri May 16 15:03:54 2008 Subject: [Histonet] AEC chromogen - coverslipping In-Reply-To: <482D8A9B020000770000C9EC@gwmail4.harthosp.org> Message-ID: <706741.94015.qm@web65709.mail.ac4.yahoo.com> I don't think you can do much, except of using a water soluble mounting medium able to solidify permanently. Ren? J. Richard Cartun wrote: If you use AEC as a chromogen for IHC staining, what are using to protect it before putting the slides into xylol for permanent coverslipping? Thanks! Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri May 16 15:08:07 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri May 16 15:08:14 2008 Subject: [Histonet] Sakura tape coverslip problem In-Reply-To: Message-ID: <897976.53776.qm@web65704.mail.ac4.yahoo.com> Christie: Put the tape with the section back over the slide. Place a glass coverslip on top held with a paper clip. Immerse the whole thing in acetone. Hopefully the plastic will dissolve leaving the tissue between the glass coverslip and the slide. Eliminate the acetone by capillarity with xylene and in the same way add the coverslipping medium. Work as gentle as you can. I have done it! Ren? J. CHRISTIE GOWAN wrote: From Linke_Noelle <@t> Allergan.com Fri May 16 15:09:59 2008 From: Linke_Noelle <@t> Allergan.com (Linke_Noelle) Date: Fri May 16 15:11:36 2008 Subject: [Histonet] AEC chromogen - coverslipping In-Reply-To: <006e01c8b789$a0f962c0$6501a8c0@DHXTS541> References: <482D8A9B020000770000C9EC@gwmail4.harthosp.org> <006e01c8b789$a0f962c0$6501a8c0@DHXTS541> Message-ID: <5C3DA4BE34AA0641BAA10A7C1478B60527D26C@IRMAIL132.irvine.allergan.com> I use BioCare's Romulin AEC.....xylene compatible, it's beautiful stuff!! Noelle No?lle Linke, MS, HTL(ASCP)QIHC Allergan, Inc 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5568 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Friday, May 16, 2008 12:19 PM To: Richard Cartun; Histonet Subject: Re: [Histonet] AEC chromogen - coverslipping Richard, We have used the liquid mounting media, Crystal Mount from Biomeda - ThermoFisher used to carry this. Do the liquid coverslip first, let it dry according to directions i.e. several ways with heat, then mount a permanent coverglass over the top of this. AEC is preserved. If you try to look at the section before mounting a permanent coverslip, it can look a bit out of focus. Make sure the media covers the section smoothly and as flat at possible to avoid the "waves" of media. Someone recently posted the contact for Biomeda on Histonet on the chance ThermoFisher is not selling Crystal Mount anymore. There are other companies that supply this type of mounting media but not sure which vendors. Good luck Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Richard Cartun" To: "Histonet" Sent: Friday, May 16, 2008 11:22 AM Subject: [Histonet] AEC chromogen - coverslipping If you use AEC as a chromogen for IHC staining, what are using to protect it before putting the slides into xylol for permanent coverslipping? Thanks! Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NMargaryan <@t> childrensmemorial.org Fri May 16 15:41:47 2008 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Fri May 16 15:39:44 2008 Subject: [Histonet] WAP ab Message-ID: HI Dears, It is late of Friday and time to go home......... But I need to find good WAP Ab for immuno. It will be appreciated if anybody could suggest the WAP Ab for immuno and what kind of Retrieval to use. Have a nice weekend, Naira Naira V. Margaryan, D.V.M., Ph.D. Research Scientist Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3363 Tel: 773-755-6340 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 From sccrshlly <@t> yahoo.com Fri May 16 17:17:28 2008 From: sccrshlly <@t> yahoo.com (Shelly Coker) Date: Fri May 16 17:17:31 2008 Subject: [Histonet] Re: Hematoxylin Shortages Message-ID: <345606.59906.qm@web90303.mail.mud.yahoo.com> I just had a demo of the Surgipath staining system, and the rep informed me that there is going to be a shortage, and as of right now, Surgipath will not be shipping their Hematoxylin to new customers, only those currently using the staining system. He did inform me that they anticipated being able to send this product out to new cutomers in August, based on information received from the production side. I would take that to imply that they plan to have this under control by August, or at least that's what I am hoping! Regards, Michelle Coker HT(ASCP)cm From sccrshlly <@t> yahoo.com Fri May 16 17:23:53 2008 From: sccrshlly <@t> yahoo.com (Shelly Coker) Date: Fri May 16 17:23:58 2008 Subject: [Histonet] Histoscreen Cassettes Message-ID: <163239.63792.qm@web90303.mail.mud.yahoo.com> Hi everyone! I have some Histoscreen cassettes I would like to dispose of. These cassettes have been in our storage for close to a year. The colors I have are blue and lilac. We currently process our specimens in a microwave processor, and these cassettes wreak havoc on our tissue. If there is anyone interested, please contact me off-list. Thanks, Michelle Coker HT(ASCP)cm From san.htin <@t> yahoo.com Fri May 16 22:30:35 2008 From: san.htin <@t> yahoo.com (San Tin) Date: Fri May 16 22:30:40 2008 Subject: [Histonet] Microwave tissue processor Message-ID: <309011.60354.qm@web55806.mail.re3.yahoo.com> Dear All, Our hospital is looking for a microwave rapid tissue processor. Any comments on any instruments aspecially Sakura Tissue TeK Xpress? Any finding with Immunohistochemistry? Please Give comments. Regards, San From sccrshlly <@t> yahoo.com Fri May 16 22:43:41 2008 From: sccrshlly <@t> yahoo.com (Shelly Coker) Date: Fri May 16 22:43:45 2008 Subject: [Histonet] Re: Histoscreen Cassettes Message-ID: <479141.33606.qm@web90305.mail.mud.yahoo.com> I have found a taker for the cassettes. Have a great weekend everyone! From rjbuesa <@t> yahoo.com Sat May 17 09:21:31 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat May 17 09:21:35 2008 Subject: [Histonet] Microwave tissue processor In-Reply-To: <309011.60354.qm@web55806.mail.re3.yahoo.com> Message-ID: <677561.10453.qm@web65709.mail.ac4.yahoo.com> Sakura Tissue Tek Xpress (the first version with 4 ovens, or the newer with only two) is a good instrument although "somewhat" pricey ($250,000 for the first and half that amount for the second). There are other less expensive alternatives that allow also a "Lean" like work flow. I am sending you an article on the subject. Ren? J. San Tin wrote: http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sprice2003 <@t> gmail.com Sat May 17 13:28:20 2008 From: sprice2003 <@t> gmail.com (Sally Price) Date: Sat May 17 13:28:30 2008 Subject: [Histonet] AEC chromogen - coverslipping Message-ID: Dr. C: Although it would requre you to obtain your AEC from anaother supplier, you might consider using Biocare's AEC, which doesn't require any special handling -- slides can be dehdrayed and cleared by routine methods. Cheers, Sally ------------------------------ Message: 2 Date: Fri, 16 May 2008 13:22:35 -0400 From: "Richard Cartun" Subject: [Histonet] AEC chromogen - coverslipping To: "Histonet" histonet@lists.utsouthwestern.edu If you use AEC as a chromogen for IHC staining, what are using to protect it before putting the slides into xylol for permanent coverslipping? Thanks! Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 From John.Spair <@t> multicare.org Sat May 17 14:21:44 2008 From: John.Spair <@t> multicare.org (John Spair) Date: Sat May 17 14:26:41 2008 Subject: [Histonet] Coverslip Tape - Sakura References: <6431D02F1Q040670-01@MMS_multicare.org> Message-ID: <61A9977919846C479389493BAE2517CA01530955@MHSEXMBX1.multicare.org> Can't you just put some mounting media on the detached coverslip and put it on a blank slide to recover it?? I've not seen too many of those problems with our stuff, and we've been using the Sakura coverslip machine for about 18 years now. We keep our slides for 20 years. Our slides are stored however, in somewhat of a climate controlled environment and I've heard this is the key to not having problems. Good luck. Message: 7 Date: Fri, 16 May 2008 18:28:10 GMT From: "tissuetech@juno.com" Subject: Re: [Histonet] Sakura tape coverslip problem To: christiegowan@msn.com Cc: histonet@lists.utsouthwestern.edu Message-ID: <20080516.132810.15454.0@webmail14.vgs.untd.com> Content-Type: text/plain; charset=windows-1252 Christie Sorry about your problem. At this time there is nothing that I know of where you can remove the tissue from the tape and recover slip. I know you are caught between a rock & a hard place, but over the years no one that I know has ever been able to retrieve and remount. Sorry I can't be of more help. Fred S - Tissue Techniques Path Labs _____________________________________________________________ Click here to save cash and find low rates on auto loans. John Spair, Manager Pathology Services LABORATORIES Northwest MultiCare Health System PO Box 5299, Tacoma WA 98415 Phone: 253-403-6090 Fax: 253-403-1357 ________________________________ "MMS " made the following annotations. ------------------------------------------------------------------------------ NOTICE: This e-mail and the attachments hereto, if any, may contain privileged and/or confidential information. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, you are hereby notified that any examination, distribution or copying of this e-mail and the attachments hereto, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by email or telephone and permanently delete this e-mail and the attachments hereto, if any, and destroy any printout thereof. MultiCare Health System, Tacoma, WA 98415 (253) 403-1000. ============================================================================== From boreades <@t> gmail.com Sat May 17 21:58:52 2008 From: boreades <@t> gmail.com (=?ISO-8859-1?Q?Sebasti=E1n_Vecchio?=) Date: Sat May 17 21:59:01 2008 Subject: [Histonet] Re: Histonet Digest, Vol 54, Issue 25 In-Reply-To: <482f0f2d.3c432c0a.0f54.ffffd2d9SMTPIN_ADDED@mx.google.com> References: <482f0f2d.3c432c0a.0f54.ffffd2d9SMTPIN_ADDED@mx.google.com> Message-ID: <8546b4020805171958i390f8c42j7487c9af5c520924@mail.gmail.com> Hi everyone, I`m looking for a protocol for microwave deparaffinizing , to eliminate the use of xylene, does any have one to submit, tanks from here in southamerica. Regards, Sebastian , histotechnology student, EUTM, fmed , UDELAR. Montevideo, Uruguay. 2008/5/17, histonet-request@lists.utsouthwestern.edu : > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Keratin-903 on Liver (Patti Loykasek) > 2. AEC chromogen - coverslipping (Richard Cartun) > 3. Sakura tape coverslip problem (CHRISTIE GOWAN) > 4. RE: fresh-frozen mouse tail sectioning > (Rachel, Rivka (NIH/NEI) [E]) > 5. Calcium oxylate stain (Mary Lloyd) > 6. unsubscribe (Danielle Crippen) > 7. Re: Sakura tape coverslip problem (tissuetech@juno.com) > 8. RE: Calcium oxylate stain (Thomas Jasper) > 9. Re: Calcium oxylate stain (Gayle Callis) > 10. AEC Chromagen - Coverslipping (Paula Pierce) > 11. Re: AEC chromogen - coverslipping (Gayle Callis) > 12. looking for c-kit (Merced Leiker) > 13. Re: AEC chromogen - coverslipping (Rene J Buesa) > 14. Re: Sakura tape coverslip problem (Rene J Buesa) > 15. RE: AEC chromogen - coverslipping (Linke_Noelle) > 16. WAP ab (Margaryan, Naira) > 17. Re: Hematoxylin Shortages (Shelly Coker) > 18. Histoscreen Cassettes (Shelly Coker) > 19. Microwave tissue processor (San Tin) > 20. Re: Histoscreen Cassettes (Shelly Coker) > 21. Re: Microwave tissue processor (Rene J Buesa) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 16 May 2008 10:22:21 -0700 > From: Patti Loykasek > Subject: Re: [Histonet] Keratin-903 on Liver > To: "Metzger, Kenneth" , > > Message-ID: > Content-Type: text/plain; charset="US-ASCII" > > Hi Ken. By referring to K-903, I believe you mean cytokeratin clone 34BE12. > The CK-903 designation is actually an old catalog number, not the name of > the keratin. Dr. Allen Gown made the 34BE12 antibody about 20 years ago. > It's a high molecular weight keratin (CK1,5,10 & 14). Just a bit of history > for you. But on to helping with your staining issues. This keratin should > stain the bile ducts in liver. What pretreatment are you using? This > antibody usually works with either an enzyme digestion or a gentle heat > retrieval in citrate. Let me know if you need more info. > > > Patti Loykasek BS, HTL, QIHC > PhenoPath Laboratories > Seattle, WA > > > > > >> One of our pathologists wants us to use K-903 on liver to tag the bile >> duct. >> Though our procedure works great on prostate we are having trouble with it >> on >> the liver..any suggestions? Thanks >> >> Ken Metzger HTL(ASCP) >> Histology Supervisor >> ARUP Laboratories >> 500 Chipeta way >> Salt Lake City, UT 84108 >> 801.583.2787 ext 3101 >> >> >> - ------------------------------------------------------------------ >> The information transmitted by this e-mail and any included >> attachments are from ARUP Laboratories and are intended only for the >> recipient. The information contained in this message is confidential >> and may constitute inside or non-public information under >> international, federal, or state securities laws, or protected health >> information and is intended only for the use of the recipient. >> Unauthorized forwarding, printing, copying, distributing, or use of >> such information is strictly prohibited and may be unlawful. If you >> are not the intended recipient, please promptly delete this e-mail >> and notify the sender of the delivery error or you may call ARUP >> Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 >> (800) 522-2787 ext. 2100 >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > This e-mail message, including any attachments, is for the sole use of the > intended recipients and may contain privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not the > intended > recipient, please contact the sender by e-mail and destroy all copies of the > original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. > at (206) 374-9000. > > > > > ------------------------------ > > Message: 2 > Date: Fri, 16 May 2008 13:22:35 -0400 > From: "Richard Cartun" > Subject: [Histonet] AEC chromogen - coverslipping > To: "Histonet" > Message-ID: <482D8A9B020000770000C9EC@gwmail4.harthosp.org> > Content-Type: text/plain; charset=US-ASCII > > If you use AEC as a chromogen for IHC staining, what are using to protect it > before putting the slides into xylol for permanent coverslipping? > > Thanks! > > Richard > > Richard W. Cartun, Ph.D. > Director, Immunopathology & Histology > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > > Confidentiality Notice > > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential or proprietary > information which is legally privileged. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are not the intended > recipient, please promptly contact the sender by reply e-mail and destroy > all copies of the original message. > > > > ------------------------------ > > Message: 3 > Date: Fri, 16 May 2008 17:24:32 +0000 > From: "CHRISTIE GOWAN" > Subject: [Histonet] Sakura tape coverslip problem > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; format=flowed > > > Does anyone have a recovery method for tape coverslips that become detatched > from the slide taking the tissue with it? I have tried several things but > have not found a really good way to do this. I called the company and they > did not have a procedure. > Thanks, > Christie Gowan > UAB Hospital > Birmingham, AL > > > > > > ------------------------------ > > Message: 4 > Date: Fri, 16 May 2008 13:56:06 -0400 > From: "Rachel, Rivka (NIH/NEI) [E]" > Subject: RE: [Histonet] fresh-frozen mouse tail sectioning > To: "Gaupp, Dina D " , > > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > Do you have to freeze or section the tissue at all? If there is significant > beta-galactosidase activity, you should be able to just put the tail tip in > X-gal and see if it turns blue, having of course appropriate positive and > negative control tails that are known to carry or not carry, respectively, > the relevant transgene. > > Rivka > > > -- > Rivka A. Rachel, MD, PhD > Staff Scientist, National Eye Institute > Neurobiology-Neurodegeneration and Repair Laboratory > Tel: 301 443-4906 > > > > -----Original Message----- > From: Gaupp, Dina D [mailto:dgaupp@tulane.edu] > Sent: Fri 5/16/2008 12:04 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] fresh-frozen mouse tail sectioning > > To Whomever This Applies: > > I am having big problems sectioning mouse tail, approximately 2mm in > thickness, vertical embedded in OCT for frozen sections. The tissue is > fresh-frozen because the principle investigator would like to detect an > enzyme x-gal. No fix, no cryopreservation - the tissue was not immersed in > any type of solution. He snipped the ends of a mouse tail & immediately > gave me the tissues. I embedded the tissue vertically in OCT & flash froze > at -80C. Upon sectioning, the tissue rolled. I could not for the life of > me get 1 section. All the tissues rolled & its so tiny to begin with that > it was hard for me to grab it. I used the anti-roll plate, hoping it would > hold the tissue in place but it still rolled. I changed temperature > settings(increase & decrease temp), thickness, angle, rubbed it with my > fingers. Everything I could think of to get a section. I asked someone > else in the lab to try & they couldn't get a section. It was like the > tissue completely separated from OCT. The principle investigator will give > me more samples & I don't want this to happen again. > > Can anyone in histoland help me, tell me what I didn't do or what I did > wrong? > > Dina D. Gaupp, BS, MT > Senior Lab Supervisor > Center for Gene Therapy, SL-99 > Tulane University Health Science Center > 1430 Tulane Ave > New Orleans, La 70112 > Lab: 504-988-1194 > dgaupp@tulane.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 5 > Date: Fri, 16 May 2008 14:04:41 -0400 > From: "Mary Lloyd" > Subject: [Histonet] Calcium oxylate stain > To: > Message-ID: > <2096B6FC591D034FA50AFFD7A42EE96506774F61@dental.dentnet.dent.ohio-state.edu> > > Content-Type: text/plain; charset="us-ascii" > > My oral pathologist is requesting a specific stain for calcium oxylate. > I have done von kossa for calcium but he wants something more specific. > I would appreciate any help. Thanks Mary > > > ------------------------------ > > Message: 6 > Date: Fri, 16 May 2008 11:07:51 -0700 > From: "Danielle Crippen" > Subject: [Histonet] unsubscribe > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > > > > > Danielle Crippen > > Morphology and Imaging Core > > x2046 > > > > > > ------------------------------ > > Message: 7 > Date: Fri, 16 May 2008 18:28:10 GMT > From: "tissuetech@juno.com" > Subject: Re: [Histonet] Sakura tape coverslip problem > To: christiegowan@msn.com > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <20080516.132810.15454.0@webmail14.vgs.untd.com> > Content-Type: text/plain; charset=windows-1252 > > Christie > Sorry about your problem. At this time there is nothing that I know of > where you can remove the tissue from the tape and recover slip. I know you > are caught between a rock & a hard place, but over the years no one that I > know has ever been able to retrieve and remount. Sorry I can't be of more > help. > Fred S - Tissue Techniques Path Labs > > _____________________________________________________________ > Click here to save cash and find low rates on auto loans. > http://thirdpartyoffers.juno.com/TGL2121/fc/Ioyw6i3ndyH35dbInkZRPhRa58lNrdsPTYWRhADbbiHInlQ8K8YNeR/?count=1234567890 > > > ------------------------------ > > Message: 8 > Date: Fri, 16 May 2008 11:41:27 -0700 > From: "Thomas Jasper" > Subject: RE: [Histonet] Calcium oxylate stain > To: "Mary Lloyd" > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > <90354A475B420441B2A0396E5008D4965E20BF@copc-sbs.COPC.local> > Content-Type: text/plain; charset="US-ASCII" > > How about Alizarin Red S? > Tom J. > > Thomas Jasper HT (ASCP) BAS > Histology Supervisor > Central Oregon Regional Pathology Services > Bend, Oregon 97701 > 541/693-2677 > tjasper@copc.net > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mary > Lloyd > Sent: Friday, May 16, 2008 11:05 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Calcium oxylate stain > > My oral pathologist is requesting a specific stain for calcium oxylate. > I have done von kossa for calcium but he wants something more specific. > I would appreciate any help. Thanks Mary > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 9 > Date: Fri, 16 May 2008 13:10:02 -0600 > From: "Gayle Callis" > Subject: Re: [Histonet] Calcium oxylate stain > To: "Mary Lloyd" , > > Message-ID: <006401c8b788$71b60a50$6501a8c0@DHXTS541> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > Pizzolata's stain for calcium oxalate. I has been discussed on Histonet in > the past, and you may be able to pick up the method on the web. Sheehan and > Hrapchak Theory and Practice of Histotechnology, second edition has the > method. Very easy to do. We ran the von Kossa on an adjacent section to > distinguish between calcium oxalate and other calcium salt deposits, and > eliminate one or the other for diagnostic purposes. > > A good calcium oxalate positive control is a dog or cat kidney that drank > antifreeze containing ethylene glycol. The crystals are a bit refractile > looking in an H&E section. A local veterinary diagnositic laboratory may > have tissues available. > > Gayle M. Callis > HTL/HT/MT(ASCP) > Bozeman MT 59715 > > > ----- Original Message ----- > From: "Mary Lloyd" > To: > Sent: Friday, May 16, 2008 12:04 PM > Subject: [Histonet] Calcium oxylate stain > > > My oral pathologist is requesting a specific stain for calcium oxylate. > I have done von kossa for calcium but he wants something more specific. > I would appreciate any help. Thanks Mary > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 10 > Date: Fri, 16 May 2008 12:12:53 -0700 (PDT) > From: Paula Pierce > Subject: [Histonet] AEC Chromagen - Coverslipping > To: Histonet > Message-ID: <150685.15173.qm@web50109.mail.re2.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > I use Crystal Mount. After rinsing to blue the hematoxylin, place a drop to > cover the section, drain excess, let dry completely, and coverslip with > resin mounting media. > Paula Pierce, HTL(ASCP)HT > > Excalibur Pathology, Inc. > 631 N. Broadway Ave. > Moore, OK 73160 > 405-570-6679 cell > 405-759-3953 lab > contact@excaliburpathology.com > www.excaliburpathology.com > > ------------------------------ > > Message: 11 > Date: Fri, 16 May 2008 13:18:31 -0600 > From: "Gayle Callis" > Subject: Re: [Histonet] AEC chromogen - coverslipping > To: "Richard Cartun" , "Histonet" > > Message-ID: <006e01c8b789$a0f962c0$6501a8c0@DHXTS541> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > Richard, > > We have used the liquid mounting media, Crystal Mount from Biomeda - > ThermoFisher used to carry this. Do the liquid coverslip first, let it dry > according to directions i.e. several ways with heat, then mount a permanent > coverglass over the top of this. AEC is preserved. > > If you try to look at the section before mounting a permanent coverslip, > it can look a bit out of focus. Make sure the media covers the section > smoothly and as flat at possible to avoid the "waves" of media. > > Someone recently posted the contact for Biomeda on Histonet on the chance > ThermoFisher is not selling Crystal Mount anymore. There are other > companies that supply this type of mounting media but not sure which > vendors. > > Good luck > > Gayle M. Callis > HTL/HT/MT(ASCP) > Bozeman MT 59715 > > ----- Original Message ----- > From: "Richard Cartun" > To: "Histonet" > Sent: Friday, May 16, 2008 11:22 AM > Subject: [Histonet] AEC chromogen - coverslipping > > > If you use AEC as a chromogen for IHC staining, what are using to protect it > before putting the slides into xylol for permanent coverslipping? > > Thanks! > > Richard > > Richard W. Cartun, Ph.D. > Director, Immunopathology & Histology > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > > Confidentiality Notice > > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential or proprietary > information which is legally privileged. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are not the intended > recipient, please promptly contact the sender by reply e-mail and destroy > all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 12 > Date: Fri, 16 May 2008 15:40:16 -0400 > From: Merced Leiker > Subject: [Histonet] looking for c-kit > To: histonet@lists.utsouthwestern.edu > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <541B786D4309163175E035B6@bchwxp2702.ad.med.buffalo.edu> > Content-Type: text/plain; charset=us-ascii; format=flowed > > I'm looking for an indirect immunofluorescence procedure for localizing > c-kit on formaldehyde-fixed, paraffin-embedded tissue. > > What type of AR do you use, and do you permeabilize? > > Thanks, > > Merced M Leiker > Research Technician II > 354 BRB (Lee Lab) / 140 Farber Hall (mail) > School of Medicine and Biomedical Sciences > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 > Ph: (716) 829-6033 > Fx: (716) 829-2725 > > > > > ------------------------------ > > Message: 13 > Date: Fri, 16 May 2008 13:03:50 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] AEC chromogen - coverslipping > To: Richard Cartun , Histonet > > Message-ID: <706741.94015.qm@web65709.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > I don't think you can do much, except of using a water soluble mounting > medium able to solidify permanently. > Ren? J. > > Richard Cartun wrote: > If you use AEC as a chromogen for IHC staining, what are using to protect > it before putting the slides into xylol for permanent coverslipping? > > Thanks! > > Richard > > Richard W. Cartun, Ph.D. > Director, Immunopathology & Histology > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > > Confidentiality Notice > > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential or proprietary > information which is legally privileged. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are not the intended > recipient, please promptly contact the sender by reply e-mail and destroy > all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 14 > Date: Fri, 16 May 2008 13:08:07 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Sakura tape coverslip problem > To: CHRISTIE GOWAN , > histonet@lists.utsouthwestern.edu > Message-ID: <897976.53776.qm@web65704.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Christie: > Put the tape with the section back over the slide. Place a glass coverslip > on top held with a paper clip. Immerse the whole thing in acetone. Hopefully > the plastic will dissolve leaving the tissue between the glass coverslip and > the slide. Eliminate the acetone by capillarity with xylene and in the same > way add the coverslipping medium. Work as gentle as you can. I have done it! > Ren? J. > > CHRISTIE GOWAN wrote: > > > > > ------------------------------ > > Message: 15 > Date: Fri, 16 May 2008 13:09:59 -0700 > From: "Linke_Noelle" > Subject: RE: [Histonet] AEC chromogen - coverslipping > To: "Gayle Callis" , "Richard Cartun" > , "Histonet" > Message-ID: > <5C3DA4BE34AA0641BAA10A7C1478B60527D26C@IRMAIL132.irvine.allergan.com> > Content-Type: text/plain; charset="iso-8859-1" > > I use BioCare's Romulin AEC.....xylene compatible, it's beautiful stuff!! > > Noelle > > No?lle Linke, MS, HTL(ASCP)QIHC > Allergan, Inc > 2525 Dupont Drive RD-2A > Irvine, CA 92612 > 714-246-5568 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis > Sent: Friday, May 16, 2008 12:19 PM > To: Richard Cartun; Histonet > Subject: Re: [Histonet] AEC chromogen - coverslipping > > Richard, > > We have used the liquid mounting media, Crystal Mount from Biomeda - > ThermoFisher used to carry this. Do the liquid coverslip first, let it dry > according to directions i.e. several ways with heat, then mount a permanent > coverglass over the top of this. AEC is preserved. > > If you try to look at the section before mounting a permanent coverslip, > it can look a bit out of focus. Make sure the media covers the section > smoothly and as flat at possible to avoid the "waves" of media. > > Someone recently posted the contact for Biomeda on Histonet on the chance > ThermoFisher is not selling Crystal Mount anymore. There are other > companies that supply this type of mounting media but not sure which > vendors. > > Good luck > > Gayle M. Callis > HTL/HT/MT(ASCP) > Bozeman MT 59715 > > ----- Original Message ----- > From: "Richard Cartun" > To: "Histonet" > Sent: Friday, May 16, 2008 11:22 AM > Subject: [Histonet] AEC chromogen - coverslipping > > > If you use AEC as a chromogen for IHC staining, what are using to protect it > before putting the slides into xylol for permanent coverslipping? > > Thanks! > > Richard > > Richard W. Cartun, Ph.D. > Director, Immunopathology & Histology > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > > Confidentiality Notice > > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential or proprietary > information which is legally privileged. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are not the intended > recipient, please promptly contact the sender by reply e-mail and destroy > all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 16 > Date: Fri, 16 May 2008 15:41:47 -0500 > From: "Margaryan, Naira" > Subject: [Histonet] WAP ab > To: > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > HI Dears, > > > > It is late of Friday and time to go home......... > > > But I need to find good WAP Ab for immuno. It will be appreciated if > anybody could suggest the WAP Ab for immuno and what kind of Retrieval > to use. > > > Have a nice weekend, > > Naira > > > > Naira V. Margaryan, D.V.M., Ph.D. > > Research Scientist > > Children's Memorial Research Center > > 2300 Children's Plaza, Box 222 > > Chicago, IL 60614-3363 > > Tel: 773-755-6340 > > Fax: 773-755-6594 > > nmargaryan@childrensmemorial.org > > > > > For Express Mail: > > CMRC, Room C.473 > > 2430 N. Halsted Street > > Chicago, IL 60614-4314 > > > > > > ------------------------------ > > Message: 17 > Date: Fri, 16 May 2008 15:17:28 -0700 (PDT) > From: Shelly Coker > Subject: [Histonet] Re: Hematoxylin Shortages > To: histonet@lists.utsouthwestern.edu > Message-ID: <345606.59906.qm@web90303.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > I just had a demo of the Surgipath staining system, and the rep informed me > that there is going to be a shortage, and as of right now, Surgipath will > not be shipping their Hematoxylin to new customers, only those currently > using the staining system. He did inform me that they anticipated being > able to send this product out to new cutomers in August, based on > information received from the production side. I would take that to imply > that they plan to have this under control by August, or at least that's what > I am hoping! > > Regards, > > Michelle Coker > HT(ASCP)cm > > > > ------------------------------ > > Message: 18 > Date: Fri, 16 May 2008 15:23:53 -0700 (PDT) > From: Shelly Coker > Subject: [Histonet] Histoscreen Cassettes > To: histonet@lists.utsouthwestern.edu > Message-ID: <163239.63792.qm@web90303.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi everyone! > > I have some Histoscreen cassettes I would like to dispose of. These > cassettes have been in our storage for close to a year. The colors I have > are blue and lilac. We currently process our specimens in a microwave > processor, and these cassettes wreak havoc on our tissue. If there is > anyone interested, please contact me off-list. > > Thanks, > > Michelle Coker > HT(ASCP)cm > > > > ------------------------------ > > Message: 19 > Date: Fri, 16 May 2008 20:30:35 -0700 (PDT) > From: San Tin > Subject: [Histonet] Microwave tissue processor > To: histonet@lists.utsouthwestern.edu > Message-ID: <309011.60354.qm@web55806.mail.re3.yahoo.com> > Content-Type: text/plain; charset="us-ascii" > > > Dear All, > > Our hospital is looking for a microwave rapid tissue processor. Any > comments on any instruments aspecially Sakura Tissue TeK Xpress? Any > finding with Immunohistochemistry? > > Please Give comments. > > > Regards, > > San > > > ------------------------------ > > Message: 20 > Date: Fri, 16 May 2008 20:43:41 -0700 (PDT) > From: Shelly Coker > Subject: [Histonet] Re: Histoscreen Cassettes > To: histonet@lists.utsouthwestern.edu > Message-ID: <479141.33606.qm@web90305.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > I have found a taker for the cassettes. Have a great weekend everyone! > > > > > > ------------------------------ > > Message: 21 > Date: Sat, 17 May 2008 07:21:31 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Microwave tissue processor > To: san.htin@yahoo.com, histonet@lists.utsouthwestern.edu > Message-ID: <677561.10453.qm@web65709.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Sakura Tissue Tek Xpress (the first version with 4 ovens, or the newer with > only two) is a good instrument although "somewhat" pricey ($250,000 for the > first and half that amount for the second). > There are other less expensive alternatives that allow also a "Lean" like > work flow. > I am sending you an article on the subject. > Ren? J. > > San Tin wrote: > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 54, Issue 25 > **************************************** > From sheila_adey <@t> hotmail.com Sun May 18 07:23:55 2008 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Sun May 18 07:24:01 2008 Subject: [Histonet] AEC chromogen - coverslipping In-Reply-To: <006e01c8b789$a0f962c0$6501a8c0@DHXTS541> References: <482D8A9B020000770000C9EC@gwmail4.harthosp.org> <006e01c8b789$a0f962c0$6501a8c0@DHXTS541> Message-ID: We were of the understanding that we could just use the aqueous mountant with the cover glass in the over and they were good to go?Sheila Adey HT MLTPort Huron HospitalMichigan> From: gayle.callis@bresnan.net> To: Rcartun@harthosp.org; histonet@lists.utsouthwestern.edu> Date: Fri, 16 May 2008 13:18:31 -0600> Subject: Re: [Histonet] AEC chromogen - coverslipping> CC: > > Richard,> > We have used the liquid mounting media, Crystal Mount from Biomeda - > ThermoFisher used to carry this. Do the liquid coverslip first, let it dry > according to directions i.e. several ways with heat, then mount a permanent > coverglass over the top of this. AEC is preserved.> > If you try to look at the section before mounting a permanent coverslip, > it can look a bit out of focus. Make sure the media covers the section > smoothly and as flat at possible to avoid the "waves" of media.> > Someone recently posted the contact for Biomeda on Histonet on the chance > ThermoFisher is not selling Crystal Mount anymore. There are other > companies that supply this type of mounting media but not sure which > vendors.> > Good luck> > Gayle M. Callis> HTL/HT/MT(ASCP)> Bozeman MT 59715> > ----- Original Message ----- > From: "Richard Cartun" > To: "Histonet" > Sent: Friday, May 16, 2008 11:22 AM> Subject: [Histonet] AEC chromogen - coverslipping> > > If you use AEC as a chromogen for IHC staining, what are using to protect it > before putting the slides into xylol for permanent coverslipping?> > Thanks!> > Richard> > Richard W. Cartun, Ph.D.> Director, Immunopathology & Histology> Assistant Director, Anatomic Pathology> Hartford Hospital> 80 Seymour Street> Hartford, CT 06102> (860) 545-1596> (860) 545-0174 Fax> > Confidentiality Notice> > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential or proprietary > information which is legally privileged. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are not the intended > recipient, please promptly contact the sender by reply e-mail and destroy > all copies of the original message.> > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ From JMacDonald <@t> mtsac.edu Sun May 18 20:51:37 2008 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Sun May 18 20:51:42 2008 Subject: [Histonet] Salary Scales In-Reply-To: <355672.50007.qm@web65701.mail.ac4.yahoo.com> Message-ID: While I agree with Renee that histotechs have to make many decisions, I don't necessarily agree that they make more decisions that other areas in the lab. I worked in the clinical lab for many years and had to make many decisions regarding the adequacy of a specimen and interpret the QC before that result could be released. Manual differentials require that the tech know the morphology of all cell types. Cross matching blood for transfusions requires interpretation before that blood can be released for transfusion to the patient. An error in cross-matching can kill the patient. I can tell you that my stress level as a Medical Technologist was much higher than my stress level as Histotechnician. There are many more examples where the knowledge and judgement of the tech will determine the outcome of patient result reporting and treatment. The pathologist does not make the final decision for the Med Tech before they release results to the clinician. We were also responsible for notifying the clinician when the patient results were critical. Jennifer Rene J Buesa Sent by: histonet-bounces@lists.utsouthwestern.edu 05/15/2008 09:22 AM To "Dawson, Glen" , histonet@lists.utsouthwestern.edu cc Subject RE: [Histonet] Salary Scales Glen: And it will keep that way until histotechs star demanding what is deserved! Have you realized that histotechs are the only specialists in the medical lab that have to make decisions all along the process? When to reject a too thick slice of tissue to assure proper processing? What part to embed to cut? Up to where trim the block discarding parts of the specimen FOR EVER?! Which section to take or which to discard FOR EVER?! When to stop differentiation in a special stain? There is no other area of the ML that has to take so many decisions, and they are better paid. And will be until the HTs decide to take action and demand what is deserved. Just my opinion (as usual!). Ren? J. "Dawson, Glen" wrote: _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Mon May 19 07:34:59 2008 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Mon May 19 07:35:14 2008 Subject: [Histonet] Salary Scales In-Reply-To: Message-ID: I think we are talking about side issues and not the bigger picture here. I believe that many histotechs return to this "salary discussion" because we are puzzled as to why histotechs are so lacking in both pay and respect. I've said it before that we are in a pickle because of 3 basic reasons. Number one would be the fact that histotechs were bench trained for so many years with no degree needed. Number two is the fact that the vast majority of histotechs are female and sexism is alive and well in our world today. Lastly, pathologists have not made a point to strengthen a histotechs place in the lab/hospital. Don't get me wrong, some Pathologists go out of their way to champion our cause but most, especially in the past, had/have a vested interest in seeing that histo-pay is kept low. The puzzling thing is that basic principles that sould govern the status/pay of a profession like histotechnology such as supply&demand or complexity of the job doesn't seem to apply in our case. The result is that we are confused as to why things don't get better for the profession and we sometimes beat our chests and say things like "we rule" or insinuate that we are more important than we are...perhaps to stroke egos that could use some stroking every once in a while. Just My Opinion, Glen Dawson BS, HT & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: Jennifer MacDonald [mailto:JMacDonald@mtsac.edu] Sent: Sunday, May 18, 2008 8:52 PM To: Rene J Buesa Cc: Dawson, Glen; histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Salary Scales While I agree with Renee that histotechs have to make many decisions, I don't necessarily agree that they make more decisions that other areas in the lab. I worked in the clinical lab for many years and had to make many decisions regarding the adequacy of a specimen and interpret the QC before that result could be released. Manual differentials require that the tech know the morphology of all cell types. Cross matching blood for transfusions requires interpretation before that blood can be released for transfusion to the patient. An error in cross-matching can kill the patient. I can tell you that my stress level as a Medical Technologist was much higher than my stress level as Histotechnician. There are many more examples where the knowledge and judgement of the tech will determine the outcome of patient result reporting and treatment. The pathologist does not make the final decision for the Med Tech before they release results to the clinician. We were also responsible for notifying the clinician when the patient results were critical. Jennifer Rene J Buesa Sent by: histonet-bounces@lists.utsouthwestern.edu 05/15/2008 09:22 AM To "Dawson, Glen" , histonet@lists.utsouthwestern.edu cc Subject RE: [Histonet] Salary Scales Glen: And it will keep that way until histotechs star demanding what is deserved! Have you realized that histotechs are the only specialists in the medical lab that have to make decisions all along the process? When to reject a too thick slice of tissue to assure proper processing? What part to embed to cut? Up to where trim the block discarding parts of the specimen FOR EVER?! Which section to take or which to discard FOR EVER?! When to stop differentiation in a special stain? There is no other area of the ML that has to take so many decisions, and they are better paid. And will be until the HTs decide to take action and demand what is deserved. Just my opinion (as usual!). Ren? J. "Dawson, Glen" wrote: _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon May 19 08:15:53 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon May 19 08:16:02 2008 Subject: [Histonet] Salary Scales In-Reply-To: Message-ID: <876618.75321.qm@web65710.mail.ac4.yahoo.com> My main issue is with the samples: in histology they are usually UNIQUE, small, solid and if lost or damaged they cannot be replaced at all. That fact makes the decisions taken regarding that sample more transcendental. Unless you are dealing with a blood sample taken during a physiological or medical crisis, any blood sample can be redrawn if damaged or lost, and that makes the samples sometimes a non issue within the ML. I am not referring to the technical part of reading a blood differential count or releasing a result. Blood counts now are done automatically and what the MT has to do is to check on flagged cells, like a cytotech reading the least "normal" cells in a PAP smear "read" by an automaton. A MT has to make sure that the controls in a run are within the established limits, and if that is the case, then it is normal to release the results that, if out of the limits, come also flagged by the instrument. What I am referring to also is that when confronted with a foreseeable workload increment the manager in the medical lab starts looking for a more efficient and productive analytical instrument, but confronted with an increment in the number of surgical cases, the manager in the histology lab can only hope to be able to hire more qualified personnel. I don' say that there are no decisions to make by the MT, what I am trying to point out is that those decisions, because of the special characteristics of the samples, have more permanent consequences in histology. I am also saying that those differences are not reflected in the salaries, always higher for the MT even when the work for the MT is automated in about 80% of the tests, and it does not reach 30% for the histology lab. Ren? J. Jennifer MacDonald wrote: While I agree with Renee that histotechs have to make many decisions, I don't necessarily agree that they make more decisions that other areas in the lab. I worked in the clinical lab for many years and had to make many decisions regarding the adequacy of a specimen and interpret the QC before that result could be released. Manual differentials require that the tech know the morphology of all cell types. Cross matching blood for transfusions requires interpretation before that blood can be released for transfusion to the patient. An error in cross-matching can kill the patient. I can tell you that my stress level as a Medical Technologist was much higher than my stress level as Histotechnician. There are many more examples where the knowledge and judgement of the tech will determine the outcome of patient result reporting and treatment. The pathologist does not make the final decision for the Med Tech before they release results to the clinician. We were also responsible for notifying the clinician when the patient results were critical. Jennifer Rene J Buesa Sent by: histonet-bounces@lists.utsouthwestern.edu 05/15/2008 09:22 AM To "Dawson, Glen" , histonet@lists.utsouthwestern.edu cc Subject RE: [Histonet] Salary Scales Glen: And it will keep that way until histotechs star demanding what is deserved! Have you realized that histotechs are the only specialists in the medical lab that have to make decisions all along the process? When to reject a too thick slice of tissue to assure proper processing? What part to embed to cut? Up to where trim the block discarding parts of the specimen FOR EVER?! Which section to take or which to discard FOR EVER?! When to stop differentiation in a special stain? There is no other area of the ML that has to take so many decisions, and they are better paid. And will be until the HTs decide to take action and demand what is deserved. Just my opinion (as usual!). Ren? J. "Dawson, Glen" wrote: _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Mon May 19 08:56:56 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon May 19 08:56:47 2008 Subject: [Histonet] Salary Scales In-Reply-To: References: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82C5B@EMAIL.archildrens.org> Glen I completely agree with you. I think you have hit the nail right on the head. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Monday, May 19, 2008 7:35 AM Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Salary Scales I think we are talking about side issues and not the bigger picture here. I believe that many histotechs return to this "salary discussion" because we are puzzled as to why histotechs are so lacking in both pay and respect. I've said it before that we are in a pickle because of 3 basic reasons. Number one would be the fact that histotechs were bench trained for so many years with no degree needed. Number two is the fact that the vast majority of histotechs are female and sexism is alive and well in our world today. Lastly, pathologists have not made a point to strengthen a histotechs place in the lab/hospital. Don't get me wrong, some Pathologists go out of their way to champion our cause but most, especially in the past, had/have a vested interest in seeing that histo-pay is kept low. The puzzling thing is that basic principles that sould govern the status/pay of a profession like histotechnology such as supply&demand or complexity of the job doesn't seem to apply in our case. The result is that we are confused as to why things don't get better for the profession and we sometimes beat our chests and say things like "we rule" or insinuate that we are more important than we are...perhaps to stroke egos that could use some stroking every once in a while. Just My Opinion, Glen Dawson BS, HT & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: Jennifer MacDonald [mailto:JMacDonald@mtsac.edu] Sent: Sunday, May 18, 2008 8:52 PM To: Rene J Buesa Cc: Dawson, Glen; histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Salary Scales While I agree with Renee that histotechs have to make many decisions, I don't necessarily agree that they make more decisions that other areas in the lab. I worked in the clinical lab for many years and had to make many decisions regarding the adequacy of a specimen and interpret the QC before that result could be released. Manual differentials require that the tech know the morphology of all cell types. Cross matching blood for transfusions requires interpretation before that blood can be released for transfusion to the patient. An error in cross-matching can kill the patient. I can tell you that my stress level as a Medical Technologist was much higher than my stress level as Histotechnician. There are many more examples where the knowledge and judgement of the tech will determine the outcome of patient result reporting and treatment. The pathologist does not make the final decision for the Med Tech before they release results to the clinician. We were also responsible for notifying the clinician when the patient results were critical. Jennifer Rene J Buesa Sent by: histonet-bounces@lists.utsouthwestern.edu 05/15/2008 09:22 AM To "Dawson, Glen" , histonet@lists.utsouthwestern.edu cc Subject RE: [Histonet] Salary Scales Glen: And it will keep that way until histotechs star demanding what is deserved! Have you realized that histotechs are the only specialists in the medical lab that have to make decisions all along the process? When to reject a too thick slice of tissue to assure proper processing? What part to embed to cut? Up to where trim the block discarding parts of the specimen FOR EVER?! Which section to take or which to discard FOR EVER?! When to stop differentiation in a special stain? There is no other area of the ML that has to take so many decisions, and they are better paid. And will be until the HTs decide to take action and demand what is deserved. Just my opinion (as usual!). Ren? J. "Dawson, Glen" wrote: _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From Rcartun <@t> harthosp.org Mon May 19 09:00:48 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Mon May 19 09:01:03 2008 Subject: [Histonet] Slide filing policy Message-ID: <48314FD0020000770000CA30@gwmail4.harthosp.org> Short of keeping all slides under "lock and key", what are labs doing to keep track of patient slides that are removed from the pathology file by residents, fellows, and attending staff? Do you have them sign-out the slides if they are going to have them for more than, say 24 hours? We are constantly looking for slides that are not in the file. Thanks. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From lblazek <@t> digestivespecialists.com Mon May 19 09:11:04 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Mon May 19 09:05:49 2008 Subject: [Histonet] job opening Message-ID: <1F937FB30BDB7C4A9F39F83FEA8D379F9F54B9@bruexchange1.digestivespecialists.com> We're growing! I'm looking for an HT (ASCP), or eligible, to join our fast growing state of the art lab team. If you're interested email me off list for more information. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com From HornHV <@t> archildrens.org Mon May 19 09:07:30 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon May 19 09:07:52 2008 Subject: [Histonet] Slide filing policy In-Reply-To: <48314FD0020000770000CA30@gwmail4.harthosp.org> References: <48314FD0020000770000CA30@gwmail4.harthosp.org> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82C60@EMAIL.archildrens.org> We use the slide markers to put in the file giving the case number and who took them. The markers are removed when the slides are refiled. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Monday, May 19, 2008 9:01 AM To: Histonet Subject: [Histonet] Slide filing policy Short of keeping all slides under "lock and key", what are labs doing to keep track of patient slides that are removed from the pathology file by residents, fellows, and attending staff? Do you have them sign-out the slides if they are going to have them for more than, say 24 hours? We are constantly looking for slides that are not in the file. Thanks. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From Charles.Embrey <@t> carle.com Mon May 19 09:52:40 2008 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Mon May 19 09:52:46 2008 Subject: [Histonet] Salary Scales In-Reply-To: References: Message-ID: <44780C571F28624DBB446DE55C4D733A1FE5BF@EXCHANGEBE1.carle.com> Glen, I think the one loophole with supply and demand that hurts histotechs the most is that there is more than one source for supply. When demand is high and supply is low, as it is in both cases for histotechs right now, pay should go up. I have watched pay increase across the country since I entered the field many years ago, but not to the point it should have. The big downfall is the alternate source of supply. I actually heard a pathologist recently say, "It would be easier to just recruit someone from McDonalds and teach them to embed blocks and cut slides." In the vast majority of labs you do not have to be a certified Histotechnician to do the job. Try to get a job in the medical lab as a lab tech without an MT or MLT and see how quickly you can be hired. I know ASCP has tightened the OJT route to require more education but until labs are forced to hire only HTs or HTLs to do the job, the alternate supply channel with exist. I'm afraid that we can shake our fist at this as much as we like but the problem is just outside our sphere of control. Chuck -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Monday, May 19, 2008 7:35 AM Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Salary Scales I think we are talking about side issues and not the bigger picture here. I believe that many histotechs return to this "salary discussion" because we are puzzled as to why histotechs are so lacking in both pay and respect. I've said it before that we are in a pickle because of 3 basic reasons. Number one would be the fact that histotechs were bench trained for so many years with no degree needed. Number two is the fact that the vast majority of histotechs are female and sexism is alive and well in our world today. Lastly, pathologists have not made a point to strengthen a histotechs place in the lab/hospital. Don't get me wrong, some Pathologists go out of their way to champion our cause but most, especially in the past, had/have a vested interest in seeing that histo-pay is kept low. The puzzling thing is that basic principles that sould govern the status/pay of a profession like histotechnology such as supply&demand or complexity of the job doesn't seem to apply in our case. The result is that we are confused as to why things don't get better for the profession and we sometimes beat our chests and say things like "we rule" or insinuate that we are more important than we are...perhaps to stroke egos that could use some stroking every once in a while. Just My Opinion, Glen Dawson BS, HT & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: Jennifer MacDonald [mailto:JMacDonald@mtsac.edu] Sent: Sunday, May 18, 2008 8:52 PM To: Rene J Buesa Cc: Dawson, Glen; histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Salary Scales While I agree with Renee that histotechs have to make many decisions, I don't necessarily agree that they make more decisions that other areas in the lab. I worked in the clinical lab for many years and had to make many decisions regarding the adequacy of a specimen and interpret the QC before that result could be released. Manual differentials require that the tech know the morphology of all cell types. Cross matching blood for transfusions requires interpretation before that blood can be released for transfusion to the patient. An error in cross-matching can kill the patient. I can tell you that my stress level as a Medical Technologist was much higher than my stress level as Histotechnician. There are many more examples where the knowledge and judgement of the tech will determine the outcome of patient result reporting and treatment. The pathologist does not make the final decision for the Med Tech before they release results to the clinician. We were also responsible for notifying the clinician when the patient results were critical. Jennifer Rene J Buesa Sent by: histonet-bounces@lists.utsouthwestern.edu 05/15/2008 09:22 AM To "Dawson, Glen" , histonet@lists.utsouthwestern.edu cc Subject RE: [Histonet] Salary Scales Glen: And it will keep that way until histotechs star demanding what is deserved! Have you realized that histotechs are the only specialists in the medical lab that have to make decisions all along the process? When to reject a too thick slice of tissue to assure proper processing? What part to embed to cut? Up to where trim the block discarding parts of the specimen FOR EVER?! Which section to take or which to discard FOR EVER?! When to stop differentiation in a special stain? There is no other area of the ML that has to take so many decisions, and they are better paid. And will be until the HTs decide to take action and demand what is deserved. Just my opinion (as usual!). Ren? J. "Dawson, Glen" wrote: _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From integrated.histo <@t> gmail.com Mon May 19 09:54:59 2008 From: integrated.histo <@t> gmail.com (Cindy DuBois) Date: Mon May 19 09:55:03 2008 Subject: [Histonet] Heat & Hematoxylin Message-ID: <5d9104a30805190754o4b85c2f8x9b0747d532d69012@mail.gmail.com> Our doctors are being very strict with the A/C. It only runs from 7am to 7pm. When we come into the lab in the morning @ 3:30 it is 82. This morning I check the high for the weekend and it was 92 in the lab. Within the last 2 weeks, the doctors are also complaining about our washed-out dull looking hematoxylin. We have increased our staining time to 7 minutes (from 5) and decreased the acid alcohol to 1 dip. All the label says on the stain bottle is to keep the stain at "controlled room temp". I have mentioned this to the doctors but all I get is "85 can be considered room temp". Does anyone have any specifications as to what temperature should be maintained in a lab? Cindy From Robert.Schoonhoven <@t> mpiresearch.com Mon May 19 09:56:27 2008 From: Robert.Schoonhoven <@t> mpiresearch.com (Robert Schoonhoven) Date: Mon May 19 09:56:34 2008 Subject: [Histonet] gerbil clitoral and prepucial glands In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82C5B@EMAIL.archildrens.org> Message-ID: Is there a gerbil expert out there?? I need to know if gerbils have clitoral and prepucial glands. TIA Robert Schoonhoven BS, HT, HTL (ASCP) Scientist, Pathology MPI Research 54943 North Main Street Mattawan, MI 49071-9399 E-Mail: robert.schoonhoven@mpiresearch.com Office 269.668.3336 X-1768 Lab. 269.668.3336 X-2009 Cell 269.615.0576 This communication, including attachments, is for the exclusive use of addressee and may contain proprietary, confidential and/or privileged information. If you are not the intended recipient, any use, copying, disclosure, dissemination or distribution is strictly prohibited. If you are not the intended recipient, please notify the sender immediately by return e-mail, delete this communication and destroy all copies. From rjbuesa <@t> yahoo.com Mon May 19 10:08:16 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon May 19 10:08:29 2008 Subject: [Histonet] Slide filing policy In-Reply-To: <48314FD0020000770000CA30@gwmail4.harthosp.org> Message-ID: <123030.96819.qm@web65711.mail.ac4.yahoo.com> We used a triple approach to this issue: 1- a log where the person receiving the slides (usually a resident) had to write down the slides received from an assistant or a clerk working at the lab, before being able to take them away. 2- the assistant or clerk removing the slides (and working from a written request), placed a cardboard divider where the slides were taken from, with the name of the person requesting the slides, along with the date, were written. 3- before requesting additional slides (unless authorized by the chief resident because it was part of a research, study or conference) any resident with slides had to return them before receiving additional slides. Also it was a task of the assistants/clerk to check the files for slides removed for more than 3 weeks. Ren? J. Richard Cartun wrote: Short of keeping all slides under "lock and key", what are labs doing to keep track of patient slides that are removed from the pathology file by residents, fellows, and attending staff? Do you have them sign-out the slides if they are going to have them for more than, say 24 hours? We are constantly looking for slides that are not in the file. Thanks. Richard Richard W. Cartun, Ph.D. From rjbuesa <@t> yahoo.com Mon May 19 10:13:02 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon May 19 10:13:11 2008 Subject: [Histonet] Slide filing policy In-Reply-To: <48314FD0020000770000CA30@gwmail4.harthosp.org> Message-ID: <81197.97696.qm@web65701.mail.ac4.yahoo.com> Sorry, I forgot a detail! We were able to do our "triple approach" to the issue of removing slides, because we had ALL our slides in cabinets in a large room, under "lock and key". The cut blocks (9 years worth of them) were in another locked room. Ren? J. Richard Cartun wrote: Short of keeping all slides under "lock and key", what are labs doing to keep track of patient slides that are removed from the pathology file by residents, fellows, and attending staff? Do you have them sign-out the slides if they are going to have them for more than, say 24 hours? We are constantly looking for slides that are not in the file. Thanks. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Mon May 19 10:26:16 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Mon May 19 10:26:23 2008 Subject: [Histonet] Slide filing policy In-Reply-To: <123030.96819.qm@web65711.mail.ac4.yahoo.com> References: <123030.96819.qm@web65711.mail.ac4.yahoo.com> Message-ID: <48319C18.3080403@pathology.washington.edu> Rene, What happens after normal hours, can a resident still checkout slides? Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Rene J Buesa wrote: > We used a triple approach to this issue: > 1- a log where the person receiving the slides (usually a resident) had to write down the slides received from an assistant or a clerk working at the lab, before being able to take them away. > 2- the assistant or clerk removing the slides (and working from a written request), placed a cardboard divider where the slides were taken from, with the name of the person requesting the slides, along with the date, were written. > 3- before requesting additional slides (unless authorized by the chief resident because it was part of a research, study or conference) any resident with slides had to return them before receiving additional slides. > > Also it was a task of the assistants/clerk to check the files for slides removed for more than 3 weeks. > Ren? J. > > Richard Cartun wrote: > Short of keeping all slides under "lock and key", what are labs doing to keep track of patient slides that are removed from the pathology file by residents, fellows, and attending staff? Do you have them sign-out the slides if they are going to have them for more than, say 24 hours? We are constantly looking for slides that are not in the file. Thanks. > > Richard > > Richard W. Cartun, Ph.D. > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From victor <@t> pathology.washington.edu Mon May 19 10:30:27 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Mon May 19 10:30:37 2008 Subject: [Histonet] Slide filing policy In-Reply-To: <48314FD0020000770000CA30@gwmail4.harthosp.org> References: <48314FD0020000770000CA30@gwmail4.harthosp.org> Message-ID: <48319D13.2090705@pathology.washington.edu> Richard, We created our own electronic tracking program. Our slides are bar coded, so it is very easy to check them out. The information is written to our LIS program as well, so anyone can see where the slides are at a glance. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Richard Cartun wrote: > Short of keeping all slides under "lock and key", what are labs doing to keep track of patient slides that are removed from the pathology file by residents, fellows, and attending staff? Do you have them sign-out the slides if they are going to have them for more than, say 24 hours? We are constantly looking for slides that are not in the file. Thanks. > > Richard > > Richard W. Cartun, Ph.D. > Director, Immunopathology & Histology > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > > Confidentiality Notice > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Mon May 19 11:18:42 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon May 19 11:18:48 2008 Subject: [Histonet] Salary Scales In-Reply-To: <44780C571F28624DBB446DE55C4D733A1FE5BF@EXCHANGEBE1.carle.com> Message-ID: <81076.91454.qm@web65708.mail.ac4.yahoo.com> Completely agree with Charles. That "McDonald hiring attitude" is similar to the one used at the start of the XXth century by pathologists all around when hiring to train in histology low pay hospital personnel. Now who SHOULD promote a correct policy of hiring licensed personnel for histology. I strongly believe that this ought to be the sole and dedicated lobbying task of the National Society for Histotechnology. Nothing else but this! Ren? J. "Charles.Embrey" wrote: Glen, I think the one loophole with supply and demand that hurts histotechs the most is that there is more than one source for supply. When demand is high and supply is low, as it is in both cases for histotechs right now, pay should go up. I have watched pay increase across the country since I entered the field many years ago, but not to the point it should have. The big downfall is the alternate source of supply. I actually heard a pathologist recently say, "It would be easier to just recruit someone from McDonalds and teach them to embed blocks and cut slides." In the vast majority of labs you do not have to be a certified Histotechnician to do the job. Try to get a job in the medical lab as a lab tech without an MT or MLT and see how quickly you can be hired. I know ASCP has tightened the OJT route to require more education but until labs are forced to hire only HTs or HTLs to do the job, the alternate supply channel with exist. I'm afraid that we can shake our fist at this as much as we like but the problem is just outside our sphere of control. Chuck -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Monday, May 19, 2008 7:35 AM Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Salary Scales I think we are talking about side issues and not the bigger picture here. I believe that many histotechs return to this "salary discussion" because we are puzzled as to why histotechs are so lacking in both pay and respect. I've said it before that we are in a pickle because of 3 basic reasons. Number one would be the fact that histotechs were bench trained for so many years with no degree needed. Number two is the fact that the vast majority of histotechs are female and sexism is alive and well in our world today. Lastly, pathologists have not made a point to strengthen a histotechs place in the lab/hospital. Don't get me wrong, some Pathologists go out of their way to champion our cause but most, especially in the past, had/have a vested interest in seeing that histo-pay is kept low. The puzzling thing is that basic principles that sould govern the status/pay of a profession like histotechnology such as supply&demand or complexity of the job doesn't seem to apply in our case. The result is that we are confused as to why things don't get better for the profession and we sometimes beat our chests and say things like "we rule" or insinuate that we are more important than we are...perhaps to stroke egos that could use some stroking every once in a while. Just My Opinion, Glen Dawson BS, HT & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: Jennifer MacDonald [mailto:JMacDonald@mtsac.edu] Sent: Sunday, May 18, 2008 8:52 PM To: Rene J Buesa Cc: Dawson, Glen; histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Salary Scales While I agree with Renee that histotechs have to make many decisions, I don't necessarily agree that they make more decisions that other areas in the lab. I worked in the clinical lab for many years and had to make many decisions regarding the adequacy of a specimen and interpret the QC before that result could be released. Manual differentials require that the tech know the morphology of all cell types. Cross matching blood for transfusions requires interpretation before that blood can be released for transfusion to the patient. An error in cross-matching can kill the patient. I can tell you that my stress level as a Medical Technologist was much higher than my stress level as Histotechnician. There are many more examples where the knowledge and judgement of the tech will determine the outcome of patient result reporting and treatment. The pathologist does not make the final decision for the Med Tech before they release results to the clinician. We were also responsible for notifying the clinician when the patient results were critical. Jennifer Rene J Buesa Sent by: histonet-bounces@lists.utsouthwestern.edu 05/15/2008 09:22 AM To "Dawson, Glen" , histonet@lists.utsouthwestern.edu cc Subject RE: [Histonet] Salary Scales Glen: And it will keep that way until histotechs star demanding what is deserved! Have you realized that histotechs are the only specialists in the medical lab that have to make decisions all along the process? When to reject a too thick slice of tissue to assure proper processing? What part to embed to cut? Up to where trim the block discarding parts of the specimen FOR EVER?! Which section to take or which to discard FOR EVER?! When to stop differentiation in a special stain? There is no other area of the ML that has to take so many decisions, and they are better paid. And will be until the HTs decide to take action and demand what is deserved. Just my opinion (as usual!). Ren? J. "Dawson, Glen" wrote: _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LeslieS <@t> vetmed.ufl.edu Mon May 19 12:21:56 2008 From: LeslieS <@t> vetmed.ufl.edu (Shawn Leslie) Date: Mon May 19 12:22:21 2008 Subject: Fwd: RE: [Histonet] Salary Scales References: <44780C571F28624DBB446DE55C4D733A1FE5BF@EXCHANGEBE1.carle.com> <81076.91454.qm@web65708.mail.ac4.yahoo.com> <48317E2A.CC0E.007B.0@vetmed.ufl.edu> Message-ID: <48317EEC.CC0E.007B.0@vetmed.ufl.edu> Fact of the matter is...We have always been "the red headed step child of the lab". I think we always will be as well. There still exists an attitude in the main lab ,and labs in general, that anyone can be a Histotech. I remember in a lab I once worked that when a new person would come in.....they would be walked through a gauntlet of people and introduced to the lab techs and of course "Mr. Leslie... he works in Histology". Didn't even get respected as a Lab tech just the person that works in Histology though I was, in fact, trained in Clinical Path before entering Anatomical Path. And what about the Pathologist, that after complaining that a piece of equipment was failing, said to me " oh you Histotechs are all alike...You drop out of life and become a Histotech and then complain about it". Again illustrating the lack of respect for the Histotech. So as long this continues it will never change..... Just my two cents worth.... Completely agree with Charles. That "McDonald hiring attitude" is similar to the one used at the start of the XXth century by pathologists all around when hiring to train in histology low pay hospital personnel. Now who SHOULD promote a correct policy of hiring licensed personnel for histology. I strongly believe that this ought to be the sole and dedicated lobbying task of the National Society for Histotechnology. Nothing else but this! Ren? J. "Charles.Embrey" wrote: Glen, I think the one loophole with supply and demand that hurts histotechs the most is that there is more than one source for supply. When demand is high and supply is low, as it is in both cases for histotechs right now, pay should go up. I have watched pay increase across the country since I entered the field many years ago, but not to the point it should have. The big downfall is the alternate source of supply. I actually heard a pathologist recently say, "It would be easier to just recruit someone from McDonalds and teach them to embed blocks and cut slides." In the vast majority of labs you do not have to be a certified Histotechnician to do the job. Try to get a job in the medical lab as a lab tech without an MT or MLT and see how quickly you can be hired. I know ASCP has tightened the OJT route to require more education but until labs are forced to hire only HTs or HTLs to do the job, the alternate supply channel with exist. I'm afraid that we can shake our fist at this as much as we like but the problem is just outside our sphere of control. Chuck -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Monday, May 19, 2008 7:35 AM Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Salary Scales I think we are talking about side issues and not the bigger picture here. I believe that many histotechs return to this "salary discussion" because we are puzzled as to why histotechs are so lacking in both pay and respect. I've said it before that we are in a pickle because of 3 basic reasons. Number one would be the fact that histotechs were bench trained for so many years with no degree needed. Number two is the fact that the vast majority of histotechs are female and sexism is alive and well in our world today. Lastly, pathologists have not made a point to strengthen a histotechs place in the lab/hospital. Don't get me wrong, some Pathologists go out of their way to champion our cause but most, especially in the past, had/have a vested interest in seeing that histo-pay is kept low. The puzzling thing is that basic principles that sould govern the status/pay of a profession like histotechnology such as supply&demand or complexity of the job doesn't seem to apply in our case. The result is that we are confused as to why things don't get better for the profession and we sometimes beat our chests and say things like "we rule" or ins inuate that we are more important than we are...perhaps to stroke egos that could use some stroking every once in a while. Just My Opinion, Glen Dawson BS, HT & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: Jennifer MacDonald [mailto:JMacDonald@mtsac.edu] Sent: Sunday, May 18, 2008 8:52 PM To: Rene J Buesa Cc: Dawson, Glen; histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Salary Scales While I agree with Renee that histotechs have to make many decisions, I don't necessarily agree that they make more decisions that other areas in the lab. I worked in the clinical lab for many years and had to make many decisions regarding the adequacy of a specimen and interpret the QC before that result could be released. Manual differentials require that the tech know the morphology of all cell types. Cross matching blood for transfusions requires interpretation before that blood can be released for transfusion to the patient. An error in cross-matching can kill the patient. I can tell you that my stress level as a Medical Technologist was much higher than my stress level as Histotechnician. There are many more examples where the knowledge and judgement of the tech will determine the outcome of patient result reporting and treatment. The pathologist does not make the final decision for the Med Tech before they release results to the clinician. We were also responsible for notifying the clinician when the patient results were critical. Jennifer Rene J Buesa Sent by: histonet-bounces@lists.utsouthwestern.edu 05/15/2008 09:22 AM To "Dawson, Glen" , histonet@lists.utsouthwestern.edu cc Subject RE: [Histonet] Salary Scales Glen: And it will keep that way until histotechs star demanding what is deserved! Have you realized that histotechs are the only specialists in the medical lab that have to make decisions all along the process? When to reject a too thick slice of tissue to assure proper processing? What part to embed to cut? Up to where trim the block discarding parts of the specimen FOR EVER?! Which section to take or which to discard FOR EVER?! When to stop differentiation in a special stain? There is no other area of the ML that has to take so many decisions, and they are better paid. And will be until the HTs decide to take action and demand what is deserved. Just my opinion (as usual!). Ren? J. "Dawson, Glen" wrote: _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Shawn Leslie HT(ASCP) Scientific Research Manager Anatomic Pathology University of Florida School of Veterinary Medicine 352-392-2235 ext 4555 From jqb7 <@t> cdc.gov Mon May 19 12:27:19 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Mon May 19 12:31:27 2008 Subject: [Histonet] Microwave tissue processor References: <309011.60354.qm@web55806.mail.re3.yahoo.com> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A73DF62B@LTA3VS011.ees.hhs.gov> We have the Xpress and it is amazingly simple to use and maintain. The only difference we found with our IHC was that the staining was a little more intense with the Xpress processed tissue with a few of the antibodies. But that is a good thing! Feel free to call or email me directly if you like. Jeanine Bartlett, HT(ASCP)QIHC Centers for Disease Control Atlanta, GA 404-639-3590 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of San Tin Sent: Fri 5/16/2008 11:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave tissue processor Dear All, Our hospital is looking for a microwave rapid tissue processor. Any comments on any instruments aspecially Sakura Tissue TeK Xpress? Any finding with Immunohistochemistry? Please Give comments. Regards, San _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Mon May 19 12:36:06 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Mon May 19 12:37:26 2008 Subject: [Histonet] Slide filing policy References: <48314FD0020000770000CA30@gwmail4.harthosp.org> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A73DF62C@LTA3VS011.ees.hhs.gov> Please share all replies...we have the same problem! Jeanine Bartlett ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Richard Cartun Sent: Mon 5/19/2008 10:00 AM To: Histonet Subject: [Histonet] Slide filing policy Short of keeping all slides under "lock and key", what are labs doing to keep track of patient slides that are removed from the pathology file by residents, fellows, and attending staff? Do you have them sign-out the slides if they are going to have them for more than, say 24 hours? We are constantly looking for slides that are not in the file. Thanks. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From klmercer <@t> MIT.EDU Mon May 19 12:49:31 2008 From: klmercer <@t> MIT.EDU (Kim L Mercer) Date: Mon May 19 12:49:48 2008 Subject: Fwd: RE: [Histonet] Salary Scales In-Reply-To: <48317EEC.CC0E.007B.0@vetmed.ufl.edu> References: <44780C571F28624DBB446DE55C4D733A1FE5BF@EXCHANGEBE1.carle.com> <81076.91454.qm@web65708.mail.ac4.yahoo.com> <48317E2A.CC0E.007B.0@vetmed.ufl.edu> <48317EEC.CC0E.007B.0@vetmed.ufl.edu> Message-ID: <20080519134931.kgcclzspcsg00wsk@webmail.mit.edu> In Ireland, Histolgy is one equal branch of medical technology (microbiology, hematology, Clinical chemistry and Blood transfusion). Just thought you'd all like to hear that.I was shocked when I came to this country an saw that histotechs were treated differently. Kim Quoting Shawn Leslie : > Fact of the matter is...We have always been "the red headed step child > of the lab". I think we always will be as well. There still exists an > attitude in the main lab ,and labs in general, that anyone can be a > Histotech. I remember in a lab I once worked that when a new person > would come in.....they would be walked through a gauntlet of people and > introduced to the lab techs and of course "Mr. Leslie... he works in > Histology". Didn't even get respected as a Lab tech just the person that > works in Histology though I was, in fact, trained in Clinical Path > before entering Anatomical Path. > And what about the Pathologist, that after complaining that a piece > of equipment was failing, said to me " oh you Histotechs are all > alike...You drop out of life and become a Histotech and then complain > about it". Again illustrating the lack of respect for the Histotech. So > as long this continues it will never change..... > > Just my two cents worth.... > > > > > Completely agree with Charles. That "McDonald hiring attitude" is > similar to the one used at the start of the XXth century by pathologists > all around when hiring to train in histology low pay hospital > personnel. > Now who SHOULD promote a correct policy of hiring licensed personnel > for histology. > I strongly believe that this ought to be the sole and dedicated > lobbying task of the National Society for Histotechnology. Nothing else > but this! > Ren? J. > > "Charles.Embrey" wrote: > Glen, I think the one loophole with supply and demand that hurts > histotechs the most is that there is more than one source for supply. > When demand is high and supply is low, as it is in both cases for > histotechs right now, pay should go up. I have watched pay increase > across the country since I entered the field many years ago, but not to > the point it should have. The big downfall is the alternate source of > supply. I actually heard a pathologist recently say, "It would be easier > to just recruit someone from McDonalds and teach them to embed blocks > and cut slides." In the vast majority of labs you do not have to be a > certified Histotechnician to do the job. Try to get a job in the medical > lab as a lab tech without an MT or MLT and see how quickly you can be > hired. I know ASCP has tightened the OJT route to require more education > but until labs are forced to hire only HTs or HTLs to do the job, the > alternate supply channel with exist. I'm afraid that we can shake our > fist > at this as much as we like but the problem is just outside our sphere > of control. > > Chuck > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Dawson, Glen > Sent: Monday, May 19, 2008 7:35 AM > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Salary Scales > > I think we are talking about side issues and not the bigger picture > here. I believe that many histotechs return to this "salary discussion" > because we are puzzled as to why histotechs are so lacking in both pay > and respect. I've said it before that we are in a pickle because of 3 > basic reasons. Number one would be the fact that histotechs were bench > trained for so many years with no degree needed. Number two is the fact > that the vast majority of histotechs are female and sexism is alive and > well in our world today. Lastly, pathologists have not made a point to > strengthen a histotechs place in the lab/hospital. Don't get me wrong, > some Pathologists go out of their way to champion our cause but most, > especially in the past, had/have a vested interest in seeing that > histo-pay is kept low. > > The puzzling thing is that basic principles that sould govern the > status/pay of a profession like histotechnology such as supply&demand or > complexity of the job doesn't seem to apply in our case. The result is > that we are confused as to why things don't get better for the > profession and we sometimes beat our chests and say things like "we > rule" or ins > inuate that we are more important than we are...perhaps to > stroke egos that could use some stroking every once in a while. > > Just My Opinion, > > Glen Dawson BS, HT & QIHC > IHC Manager > Milwaukee, WI > > -----Original Message----- > From: Jennifer MacDonald [mailto:JMacDonald@mtsac.edu] > Sent: Sunday, May 18, 2008 8:52 PM > To: Rene J Buesa > Cc: Dawson, Glen; histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Subject: RE: [Histonet] Salary Scales > > > > While I agree with Renee that histotechs have to make many decisions, I > don't necessarily agree that they make more decisions that other areas > in the lab. I worked in the clinical lab for many years and had to make > many decisions regarding the adequacy of a specimen and interpret the QC > before that result could be released. Manual differentials require that > the tech know the morphology of all cell types. Cross matching blood for > transfusions requires interpretation before that blood can be released > for transfusion to the patient. An error in cross-matching can kill the > patient. I can tell you that my stress level as a Medical Technologist > was much higher than my stress level as Histotechnician. There are many > more examples where the knowledge and judgement of the tech will > determine the outcome of patient result reporting and treatment. The > pathologist does not make the final decision for the Med Tech before > they release results to the clinician. We were also responsible > for notifying the clinician when the patient results were critical. > Jennifer > > > > > > Rene J Buesa > Sent by: histonet-bounces@lists.utsouthwestern.edu > > > 05/15/2008 09:22 AM > > > To > "Dawson, Glen" , histonet@lists.utsouthwestern.edu > > cc > > Subject > RE: [Histonet] Salary Scales > > > > > > > Glen: > And it will keep that way until histotechs star demanding what is > deserved! > > Have you realized that histotechs are the only specialists in the > medical lab that have to make decisions all along the process? > When to reject a too thick slice of tissue to assure proper > processing? > What part to embed to cut? > Up to where trim the block discarding parts of the specimen FOR EVER?! > Which section to take or which to discard FOR EVER?! > When to stop differentiation in a special stain? > > There is no other area of the ML that has to take so many decisions, > and they are better paid. And will be until the HTs decide to take > action and demand what is deserved. > Just my opinion (as usual!). > Ren? J. > > > "Dawson, Glen" wrote: > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Shawn Leslie HT(ASCP) > Scientific Research Manager > Anatomic Pathology > University of Florida > School of Veterinary Medicine > 352-392-2235 ext 4555 > From NSEARCY <@t> swmail.sw.org Mon May 19 12:53:25 2008 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Mon May 19 12:53:38 2008 Subject: [Histonet] Training For Transcriptionist Message-ID: Historically, pathology transcriptionists (and I assume radiology transcriptionists) have been trained on- the job. I am in dire need of some on-line resources for training of new employees. There are no formal programs---only 'terminology" courses at junior colleges. Anyone use these types of aids or know of how one replaces these valuable people? This has always been an issue for me. Any comments? Thanks From Janet.Bonner <@t> FLHOSP.ORG Mon May 19 13:02:47 2008 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Mon May 19 13:05:43 2008 Subject: [Histonet] Slide filing policy References: <48314FD0020000770000CA30@gwmail4.harthosp.org> <1CE1847DFEA0A647B1CCDE4108EA60A73DF62C@LTA3VS011.ees.hhs.gov> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F266D@fhosxchmb006.ADVENTISTCORP.NET> We have them in a separate building, under lock and key, with request made in advance (by Email is fine). They belong to the hospital. A tight control will save you from Litigation one day! Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Mon 5/19/2008 1:36 PM To: Richard Cartun; Histonet Subject: RE: [Histonet] Slide filing policy Please share all replies...we have the same problem! Jeanine Bartlett ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Richard Cartun Sent: Mon 5/19/2008 10:00 AM To: Histonet Subject: [Histonet] Slide filing policy Short of keeping all slides under "lock and key", what are labs doing to keep track of patient slides that are removed from the pathology file by residents, fellows, and attending staff? Do you have them sign-out the slides if they are going to have them for more than, say 24 hours? We are constantly looking for slides that are not in the file. Thanks. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From vazquezr <@t> ohsu.edu Mon May 19 13:08:26 2008 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Mon May 19 13:09:10 2008 Subject: [Histonet] Heat & Hematoxylin Message-ID: Cindy, Do you have refrig/freezers, cryostats, and anything that puts out heat from the motor? Our lab is between 68-70, our equipment doesn't have to work to stay at their temps. And the equipment will last longer. And beside the tech have to be comfortable. It would be inconsiderate to employees to have them suffer in a high temp such as 85 degrees. Does the doctors have to stay in the lab constantly? Crank their offices heat up to 85 and see how they like it, I bet not. Just my opinion. Robyn >>> "Cindy DuBois" 5/19/2008 7:54 AM >>> Our doctors are being very strict with the A/C. It only runs from 7am to 7pm. When we come into the lab in the morning @ 3:30 it is 82. This morning I check the high for the weekend and it was 92 in the lab. Within the last 2 weeks, the doctors are also complaining about our washed-out dull looking hematoxylin. We have increased our staining time to 7 minutes (from 5) and decreased the acid alcohol to 1 dip. All the label says on the stain bottle is to keep the stain at "controlled room temp". I have mentioned this to the doctors but all I get is "85 can be considered room temp". Does anyone have any specifications as to what temperature should be maintained in a lab? Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From plucas <@t> biopath.org Mon May 19 13:22:25 2008 From: plucas <@t> biopath.org (Paula Lucas) Date: Mon May 19 13:22:28 2008 Subject: [Histonet] Histotechs needed Message-ID: <20080519182225.6074C549D@alexander.cnchost.com> Hello, We need a histotech who can embed and cut on Saturday mornings from 5 am to about 10 am. We are also looking for a part time histotech who can embed and cut from Tuesday through Saturday mornings from 5 am to about 10 am. We are in Fountain Valley, California. Please email me if interested or if you have any questions. Thank you, Paula Lucas Lab Manager Bio-Path Medical Group From tim.morken <@t> thermofisher.com Mon May 19 13:27:26 2008 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Mon May 19 13:27:58 2008 Subject: Fwd: RE: [Histonet] Salary Scales In-Reply-To: <20080519134931.kgcclzspcsg00wsk@webmail.mit.edu> References: <44780C571F28624DBB446DE55C4D733A1FE5BF@EXCHANGEBE1.carle.com><81076.91454.qm@web65708.mail.ac4.yahoo.com><48317E2A.CC0E.007B.0@vetmed.ufl.edu><48317EEC.CC0E.007B.0@vetmed.ufl.edu> <20080519134931.kgcclzspcsg00wsk@webmail.mit.edu> Message-ID: <6BFF6D137DF6BC43B33891BA96E83B19017BCF95@PGHCR-EXMB-VS-1.na.fshrnet.com> Kim wrote " In Ireland, Histology is one equal branch of medical technology (microbiology, hematology, Clinical chemistry and Blood transfusion)." Yes, in most of the world Histotechnology is "just" another branch of medical technology. I was exposed to that while working in Saudi Arabia and found that I was a bit embarassed about the education level of the US techs vs the techs from the rest of the world (we had 10 nationalities working in our lab and every one besides the US was Bachelors-level educated with Histotechnology as a specialty - and all were excellent). Somewhere along the line in the distant past the U.S. Med Tech schools and associations dropped Histology as a "legitimate" discipline and we have been paying for it every since. I suspect it had to do with the fact that licensed med techs can report out results - even if it is just numbers it gives them responsibility no Histotech has. Since Pathologists do all the reporting out of histology the histotech is seen as a worker, not a scientist. It doesn't help that for many years, (even now in some places) people were practically dragged off the street to do histology work. Of course pathologists have fought licensing for histotechs forever - essentially a financial issue for those private labs that train their own. I know techs in private labs who say their pathologists are unwilling to support working towards an HT certification because if the person gets it they either will want more pay or will leave to get higher pay. Of course we on this list all know that in the last 25 years hisotechnology has come up to the level of any med tech area in terms of technology. But, we still don't report anything out. We do complicated technical work, but unfortunately it is still seen as an area where a reasonably dexterous person can do the work without much knowledge. What I have seen is that those histotechs who are willing to do the extra work to learn the field at the expert level (that is, able to advise pathologists, not just take orders) are quite well off in terms of ability to command the pay they want and the job they want. It does take work, and some sacrifice, but well worth it in the long run if this is the field you will be in for many years. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim L Mercer Sent: Monday, May 19, 2008 10:50 AM To: histonet@lists.utsouthwestern.edu Subject: Re: Fwd: RE: [Histonet] Salary Scales In Ireland, Histolgy is one equal branch of medical technology (microbiology, hematology, Clinical chemistry and Blood transfusion). Just thought you'd all like to hear that.I was shocked when I came to this country an saw that histotechs were treated differently. Kim From LuckG <@t> empirehealth.org Mon May 19 13:36:25 2008 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Mon May 19 13:36:31 2008 Subject: [Histonet] Slide filing policy>> Another Reply In-Reply-To: <5F31F38C96781A4FBE3196EBC22D47807F266D@fhosxchmb006.ADVENTISTCORP.NET> References: <48314FD0020000770000CA30@gwmail4.harthosp.org><1CE1847DFEA0A647B1CCDE4108EA60A73DF62C@LTA3VS011.ees.hhs.gov> <5F31F38C96781A4FBE3196EBC22D47807F266D@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: <6BB8BC4519AAB844B174FC739A679BBCCF00FB@IRMEXCH01.irm.inhs.org> Our blocks and slides are locked up as well. Any requests are processed through the transcription staff and are checked out in a log book. In addition an electronic "marker" is attached to the case in Meditech. This filter is removed when the materials are returned. We do searches on a regular basis to look for long standing attached markers that may need a follow-up. Greg Luck, B.S., HT(ASCP) Anatomic Pathology Supervisor Deaconess Med Cntr 800 W. 5th Ave Spokane, WA 99204 Offc 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmc.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonner, Janet Sent: Monday, May 19, 2008 11:03 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Richard Cartun; Histonet Subject: RE: [Histonet] Slide filing policy We have them in a separate building, under lock and key, with request made in advance (by Email is fine). They belong to the hospital. A tight control will save you from Litigation one day! Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Mon 5/19/2008 1:36 PM To: Richard Cartun; Histonet Subject: RE: [Histonet] Slide filing policy Please share all replies...we have the same problem! Jeanine Bartlett ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Richard Cartun Sent: Mon 5/19/2008 10:00 AM To: Histonet Subject: [Histonet] Slide filing policy Short of keeping all slides under "lock and key", what are labs doing to keep track of patient slides that are removed from the pathology file by residents, fellows, and attending staff? Do you have them sign-out the slides if they are going to have them for more than, say 24 hours? We are constantly looking for slides that are not in the file. Thanks. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmjohnson34 <@t> hotmail.com Mon May 19 13:55:26 2008 From: jmjohnson34 <@t> hotmail.com (Jennifer Johnson) Date: Mon May 19 13:55:30 2008 Subject: [Histonet] Formalin+Acetic Acid Message-ID: Does anyone know of the top of his/her head what the chemical reaction is between formaldehyde and acetic acid that causes the milky white turbitity? Thanks, Jennifer _________________________________________________________________ Keep your kids safer online with Windows Live Family Safety. http://www.windowslive.com/family_safety/overview.html?ocid=TXT_TAGLM_WL_Refresh_family_safety_052008 From tjasper <@t> copc.net Mon May 19 14:00:31 2008 From: tjasper <@t> copc.net (Thomas Jasper) Date: Mon May 19 14:00:38 2008 Subject: [Histonet] Salary Scales References: <876618.75321.qm@web65710.mail.ac4.yahoo.com> Message-ID: <90354A475B420441B2A0396E5008D4965E20C3@copc-sbs.COPC.local> Rene, Glen, et al, I agree totally, and Rene that was a great response to Jennifer. I certainly don't have a solution or solutions to the low pay problem, but understanding it as best we can is necessary for improvement and remediation. And I think it was Chuck who pointed out that there has been some improvement. That is correct and a good thing too. I believe the point has been made about education and that is critical. The Histology world has made tremendous strides educationally. This may be preaching to the choir a bit, but hopefully communicating in this forum will help those indirectly involved with Histology (technically), yet directly involved financially, get a better handle on just how valuable we are. Rene, your points are well made. The clinical lab mind-set, for the most part, is that Histology is a walk in the park. While the rest of the clinical lab (for some reason) is at a higher level than Histology. I found Jennifer's point, about pathologists not making final decisions, before releasing results to clinicians, a bit curious. As a Med Tech, you are not diagnosing a patient, the clinician is. As a Histotech, you are not diagnosing the patient, the clinician is as well, albeit with input from a pathologist, via path report, etc. At times, clinicians are not diagnosing off clinical lab results, until there is input from a pathologist as well. That is why pathologists are the medical directors of clinical labs, even though their closest working relationships are usually with Histology. Also, the world of Histology has exploded, exponentially in the last 20-30 years. This is not to say that the clinical lab world hasn't ramped up technically as well. To the contrary, all lab service has taken tremendous technical leaps. However, automation has more readily suited the clinical lab for the most part. This has put even more pressure on Histology to meet the demands for turnaround times and staff shortages. Even though we've got great new, innovative technology there is a manual and artistic element to Histology that does not exist in the clinical lab. I've been told, the day is coming when automation will do Histology from beginning to end. In the meantime, you'd be hard pressed to replace a deft hand and a keen eye in most Histology applications. Jennifer, believe me, I'm glad your stress has been reduced doing Histology instead of clinical lab work. I take that as a compliment, a compliment to the nature of our work and the type of people doing it. Despite the money differences, I can't tell you how many times I've thanked my lucky stars to be working in Histology and with Histologists. I know this is anecdotal, but in my experience, most of Histology folks just haven't been wound as tight as other laboratorians I've encountered. Getting back to stress for a moment...having a surgeon and/or a pathologist pacing behind you while cutting a difficult frozen is a unique sensation never felt in the clinical lab. Same thing applies to any Moh's service. Lastly, I believe, more is being asked of Histology today than ever before. More...learning more in shorter periods of time, doing more with less (staff and money) and having more of an impact in patient diagnoses and avenues of treatment. The irony is that some people just expect this...continued improvement, increased workload, etc. Yet, Histologists are supposed to just keep quiet, and be thankful for what compensation comes their way. At my last job, unionization was leveraged to help alleviate this situation. I was on the other side of the fence, but could certainly appreciate the wherefore and the why. Please understand, my comments are not meant to minimize the importance of clinical labs or the hard working personnel in them. I'm trying to help shine a light onto the much deserving Histologist and the Histology world. I'm off my soapbox now, thanks. Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, May 19, 2008 6:16 AM To: Jennifer MacDonald Cc: Dawson, Glen; histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Salary Scales My main issue is with the samples: in histology they are usually UNIQUE, small, solid and if lost or damaged they cannot be replaced at all. That fact makes the decisions taken regarding that sample more transcendental. Unless you are dealing with a blood sample taken during a physiological or medical crisis, any blood sample can be redrawn if damaged or lost, and that makes the samples sometimes a non issue within the ML. I am not referring to the technical part of reading a blood differential count or releasing a result. Blood counts now are done automatically and what the MT has to do is to check on flagged cells, like a cytotech reading the least "normal" cells in a PAP smear "read" by an automaton. A MT has to make sure that the controls in a run are within the established limits, and if that is the case, then it is normal to release the results that, if out of the limits, come also flagged by the instrument. What I am referring to also is that when confronted with a foreseeable workload increment the manager in the medical lab starts looking for a more efficient and productive analytical instrument, but confronted with an increment in the number of surgical cases, the manager in the histology lab can only hope to be able to hire more qualified personnel. I don' say that there are no decisions to make by the MT, what I am trying to point out is that those decisions, because of the special characteristics of the samples, have more permanent consequences in histology. I am also saying that those differences are not reflected in the salaries, always higher for the MT even when the work for the MT is automated in about 80% of the tests, and it does not reach 30% for the histology lab. Ren? J. Jennifer MacDonald wrote: While I agree with Renee that histotechs have to make many decisions, I don't necessarily agree that they make more decisions that other areas in the lab. I worked in the clinical lab for many years and had to make many decisions regarding the adequacy of a specimen and interpret the QC before that result could be released. Manual differentials require that the tech know the morphology of all cell types. Cross matching blood for transfusions requires interpretation before that blood can be released for transfusion to the patient. An error in cross-matching can kill the patient. I can tell you that my stress level as a Medical Technologist was much higher than my stress level as Histotechnician. There are many more examples where the knowledge and judgement of the tech will determine the outcome of patient result reporting and treatment. The pathologist does not make the final decision for the Med Tech before they release results to the clinician. We were also responsible for notifying the clinician when the patient results were critical. Jennifer Rene J Buesa Sent by: histonet-bounces@lists.utsouthwestern.edu 05/15/2008 09:22 AM To "Dawson, Glen" , histonet@lists.utsouthwestern.edu cc Subject RE: [Histonet] Salary Scales Glen: And it will keep that way until histotechs star demanding what is deserved! Have you realized that histotechs are the only specialists in the medical lab that have to make decisions all along the process? When to reject a too thick slice of tissue to assure proper processing? What part to embed to cut? Up to where trim the block discarding parts of the specimen FOR EVER?! Which section to take or which to discard FOR EVER?! When to stop differentiation in a special stain? There is no other area of the ML that has to take so many decisions, and they are better paid. And will be until the HTs decide to take action and demand what is deserved. Just my opinion (as usual!). Ren? J. "Dawson, Glen" wrote: _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon May 19 14:13:34 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon May 19 14:13:39 2008 Subject: [Histonet] Heat & Hematoxylin In-Reply-To: <5d9104a30805190754o4b85c2f8x9b0747d532d69012@mail.gmail.com> Message-ID: <125757.28833.qm@web65706.mail.ac4.yahoo.com> You could do something to try to prove your point (about damage because of high temperature). Take one bottle of hematoxylin to your house (that I assume is at "good" temp.) this Friday. Come Monday cut an extra set of slides and stain one set with the hematoxylin left in the lab without A/C during the whole weekend, and another set with the hematoxylin you took to your house. Compare them and decide who is right about this issue. Ren? J. Cindy DuBois wrote: From mcauliff <@t> umdnj.edu Mon May 19 14:14:37 2008 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Mon May 19 14:14:25 2008 Subject: [Histonet] Formalin+Acetic Acid In-Reply-To: References: Message-ID: <4831D19D.5060203@umdnj.edu> There should not be any "milky white turbidity". Geoff Jennifer Johnson wrote: > Does anyone know of the top of his/her head what the chemical reaction is between formaldehyde and acetic acid that causes the milky white turbitity? > > Thanks, > Jennifer > _________________________________________________________________ > Keep your kids safer online with Windows Live Family Safety. > http://www.windowslive.com/family_safety/overview.html?ocid=TXT_TAGLM_WL_Refresh_family_safety_052008_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From MLashus <@t> pathgroup.com Mon May 19 14:20:52 2008 From: MLashus <@t> pathgroup.com (Mighnon Lashus) Date: Mon May 19 14:20:57 2008 Subject: [Histonet] Varicella zoster Message-ID: <197CD0B02A81F94994A285C59C8AE05C027A94C3F8@pgnexchange.pathgroup.com> Does anyone know where I can get an antibody for varicella zoster? Thanks, Mighnon Lashus, HT (ASCP) PathGroup Lab 4071 S. Access Road, Suite 107 Chattanooga, TN 37406 423-493-0207 423-493-0208 fax mlashus@pathgroup.com ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From gayle.callis <@t> bresnan.net Mon May 19 14:32:32 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Mon May 19 14:34:00 2008 Subject: Long reply Re: [Histonet] Heat & Hematoxylin References: Message-ID: <002a01c8b9e7$15921be0$6501a8c0@DHXTS541> Cindy and Robyn, We maintain a working temperature to approx 70 - 72F. Their suggested temperature range is outrageous. Your pathologists might find employees more productive with cooler working temperatures (you have to wear lab coats, gloves etc that add to discomfort). At the risk of being "catty", I presume your pathologists are on the "AC'd 7 am to 7 pm" schedule? What is the reasoning behind lowering temperature in that 12 hour period other than saving the almighty dollar. If the lab is that hot when the AC kicks in, isn't the system working harder (costing more) to cool down a lab? Shouldn't ALL employees have the same working conditions, including the early morning crew. Fair is fair! I suggest you survey your chemicals and solvents, list the flash point and proper storage temperature for volatile, flammable and potentially explosive chemicals you have on hand. You safety can be compromised due to improper temperatures for chemical storage, usage/longevity. 85F to 92F is excessive. High temperature also affects how paraffin sections. Contact the hematoxylin manufacturer technical services, tell them of your plight and ask them if higher temperature is a problem. They put "controlled room temperature" on their containers for a very good reason but have them say why they put storage conditions on the bottle. Controlled room temperature, in one instance, had a range of 20 - 25C (68 - 77F). This range falls into what Wikipedia defined (see below). Heat accelerates chemical reactions, and probably contributes to the breakdown of chemical solutions too. The Wikipedia definition of Room Temperature was interesting: Room temperature (also referred to as ambient temperature) is a common term to denote a certain temperature within enclosed space at which humans are accustomed. Room temperature is thus often indicated by general human comfort, with the common range of 18?C (64.4 ?F) to 24?C (75.2 ?F), though climate may acclimatise people to higher or lower temperatures. The term can also refer to a temperature of food to be consumed (e.g., red wine) which is placed in such a room for a given time. Furthermore, it may refer to a certain temperature within settings of scientific experiments and calculations. For human comfort, desirable room temperature greatly depends on individual needs and various other factors. According to the West Midlands Public Health Observatory,[1] 21 ?C (69.8 ?F) is the recommended living room temperature, whereas 18 ?C (64.4 ?F) is the recommended bedroom temperature. A study carried out at the Uppsala University,[2] on indoor air quality and subjective indoor air quality (SIAQ) in primary schools, states that perception of high room temperature was related to a poor climate of cooperation. To achieve a good SIAQ, it recommends room temperature should be at a maximum of 24.0 ?C (75.5 ?F). Scientific definition For scientific calculations, room temperature is taken to be 20 to 23.5 degrees Celsius, 528 to 537 degrees Rankine (?R), or 293 to 296 Kelvin (K), with an average of 21 ?C, about 70 degrees Fahrenheit (?F).[3]. For numerical convenience, either 20 ?C or 300 K is often used. However, room temperature is not a precisely defined scientific term as opposed to Standard Temperature and Pressure, which has several, slightly different, definitions. Good luck on solving this problem. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Robyn Vazquez" To: ; Sent: Monday, May 19, 2008 12:08 PM Subject: Re: [Histonet] Heat & Hematoxylin > Cindy, > Do you have refrig/freezers, cryostats, and anything that puts out heat > from the motor? Our lab is between 68-70, our equipment doesn't have to > work to stay at their temps. And the equipment will last longer. And > beside the tech have to be comfortable. It would be inconsiderate to > employees to have them suffer in a high temp such as 85 degrees. Does > the doctors have to stay in the lab constantly? Crank their offices heat > up to 85 and see how they like it, I bet not. Just my opinion. > > Robyn > >>>> "Cindy DuBois" 5/19/2008 7:54 AM >>> > > Our doctors are being very strict with the A/C. It only runs from 7am > to > 7pm. When we come into the lab in the morning @ 3:30 it is 82. This > morning I check the high for the weekend and it was 92 in the lab. > Within the last 2 weeks, the doctors are also complaining about our > washed-out dull looking hematoxylin. We have increased our staining > time to > 7 minutes (from 5) and decreased the acid alcohol to 1 dip. All the > label > says on the stain bottle is to keep the stain at "controlled room > temp". I > have mentioned this to the doctors but all I get is "85 can be > considered > room temp". > Does anyone have any specifications as to what temperature should be > maintained in a lab? > Cindy > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From boreades <@t> gmail.com Mon May 19 14:36:21 2008 From: boreades <@t> gmail.com (=?ISO-8859-1?Q?Sebasti=E1n_Vecchio?=) Date: Mon May 19 14:36:25 2008 Subject: [Histonet] Microwave deparaffinization. Message-ID: <8546b4020805191236y3c9cad39k8f6f1bd3a2f3302b@mail.gmail.com> I have been trying to deparafinize section in the lab, but I cannot get the right time and power for the MW oven, does any one has a protocol for this . This method eliminates the uses of xylol in the processing of staining sections. Thanks. Sebastian, Student of Histotechnology, EUTM, FMED, UDELAR. From PMonfils <@t> Lifespan.org Mon May 19 14:56:33 2008 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Mon May 19 14:56:38 2008 Subject: [Histonet] Formalin+Acetic Acid In-Reply-To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E273D7E@LSRIEXCH1.lsmaster.lifespan.org> Formaldehyde and acetic acid should not react. The two are used in combination in a number of fixatives, including Bouin's fluid and several plant fixatives, and yield clear solutions. From rjbuesa <@t> yahoo.com Mon May 19 15:48:42 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon May 19 15:48:47 2008 Subject: [Histonet] Microwave deparaffinization. In-Reply-To: <8546b4020805191236y3c9cad39k8f6f1bd3a2f3302b@mail.gmail.com> Message-ID: <549014.59137.qm@web65710.mail.ac4.yahoo.com> Paraffin is "transparent" or "translucent" to microwaves, meaning that paraffin CANNOT be melted or even heated using microwaves. Instruments using microwaves heat the container (made of or including parts made of WEFLON) that melt the paraffin by convection from the hot container but NOT because the paraffin is directly heated. This is one of those good ideas that will not fly! Ren? J. Sebasti?n Vecchio wrote: I have been trying to deparafinize section in the lab, but I cannot get the right time and power for the MW oven, does any one has a protocol for this . This method eliminates the uses of xylol in the processing of staining sections. Thanks. Sebastian, Student of Histotechnology, EUTM, FMED, UDELAR. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From saby_joseph_a <@t> yahoo.com Mon May 19 16:31:24 2008 From: saby_joseph_a <@t> yahoo.com (Joseph Saby) Date: Mon May 19 16:31:28 2008 Subject: Long reply Re: [Histonet] Heat & Hematoxylin Message-ID: <531515.2157.qm@web33801.mail.mud.yahoo.com> Another quick consideration: Most GLP labs require that the evvironment be controlled, and I would suspect the same for hospital? labs.? I could see temeprature variations causing major inconsistencies in special stains/histochemical reactions. Jose Saby, BS HT ----- Original Message ---- From: Gayle Callis To: Robyn Vazquez ; integrated.histo@gmail.com; histonet@lists.utsouthwestern.edu Sent: Monday, May 19, 2008 3:32:32 PM Subject: Long reply Re: [Histonet] Heat & Hematoxylin Cindy and Robyn, We maintain a working temperature to approx 70 - 72F.? Their suggested temperature range is outrageous.? Your pathologists might find employees more productive with cooler working temperatures (you have to wear lab coats, gloves etc that add to discomfort).? At the risk of being "catty",? I presume your pathologists? are on the "AC'd 7 am to 7 pm" schedule?? What is the reasoning behind lowering temperature in that 12 hour period other than saving the almighty dollar.? If the lab is that hot when the AC kicks in, isn't the system working harder (costing more) to cool down a lab? Shouldn't ALL employees have the same working conditions, including the early morning crew.? Fair is fair! I suggest you survey your chemicals and solvents,? list the flash point and proper storage temperature for volatile, flammable and potentially explosive chemicals you have on hand.? You safety can be compromised due to improper temperatures for chemical storage, usage/longevity.? 85F to 92F is excessive.? High temperature also affects how paraffin sections. Contact the hematoxylin manufacturer technical services, tell them of your plight and ask them if higher temperature is a problem.? They put "controlled room temperature" on their containers for a very good reason but have them say why they put storage conditions on the bottle.? Controlled room temperature, in one instance, had a range of 20 - 25C (68 - 77F).? This range falls into what Wikipedia defined (see below).? Heat accelerates chemical reactions, and probably contributes to the breakdown of chemical solutions too. The Wikipedia definition of Room Temperature was interesting: Room temperature (also referred to as ambient temperature) is a common term to denote a certain temperature within enclosed space at which humans are accustomed. Room temperature is thus often indicated by general human comfort, with the common range of 18?C (64.4 ?F) to 24?C (75.2 ?F), though climate may acclimatise people to higher or lower temperatures. The term can also refer to a temperature of food to be consumed (e.g., red wine) which is placed in such a room for a given time. Furthermore, it may refer to a certain temperature within settings of scientific experiments and calculations. For human comfort, desirable room temperature greatly depends on individual needs and various other factors. According to the West Midlands Public Health Observatory,[1] 21 ?C (69.8 ?F) is the recommended living room temperature, whereas 18 ?C (64.4 ?F) is the recommended bedroom temperature. A study carried out at the Uppsala University,[2] on indoor air quality and subjective indoor air quality (SIAQ) in primary schools, states that perception of high room temperature was related to a poor climate of cooperation. To achieve a good SIAQ, it recommends room temperature should be at a maximum of 24.0 ?C (75.5 ?F). Scientific definition For scientific calculations, room temperature is taken to be 20 to 23.5 degrees Celsius, 528 to 537 degrees Rankine (?R), or 293 to 296 Kelvin (K), with an average of 21 ?C, about 70 degrees Fahrenheit (?F).[3]. For numerical convenience, either 20 ?C or 300 K is often used. However, room temperature is not a precisely defined scientific term as opposed to Standard Temperature and Pressure, which has several, slightly different, definitions. Good luck on solving this problem. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Robyn Vazquez" To: ; Sent: Monday, May 19, 2008 12:08 PM Subject: Re: [Histonet] Heat & Hematoxylin > Cindy, > Do you have refrig/freezers, cryostats, and anything that puts out heat > from the motor?? Our lab is between 68-70, our equipment doesn't have to > work to stay at their temps.? And the equipment will last longer.? And > beside the tech have to be comfortable.? It would be inconsiderate to > employees to have them suffer in a high temp such as 85 degrees. Does > the doctors have to stay in the lab constantly? Crank their offices heat > up to 85 and see how they like it, I bet not.? Just my opinion. > > Robyn > >>>> "Cindy DuBois" 5/19/2008 7:54 AM >>> > > Our doctors are being very strict with the A/C.? It only runs from 7am > to > 7pm.? When we come into the lab in the morning @ 3:30 it is 82.? This > morning I check the high for the weekend and it was 92 in the lab. > Within the last 2 weeks, the doctors are also complaining about our > washed-out dull looking hematoxylin.? We have increased our staining > time to > 7 minutes (from 5) and decreased the acid alcohol to 1 dip.? All the > label > says on the stain bottle is to keep the stain at "controlled room > temp".? I > have mentioned this to the doctors but all I get is "85 can be > considered > room temp". > Does anyone have any specifications as to what temperature should be > maintained in a lab? > Cindy > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PIXLEYSK <@t> UCMAIL.UC.EDU Mon May 19 16:36:12 2008 From: PIXLEYSK <@t> UCMAIL.UC.EDU (Pixley, Sarah (pixleysk)) Date: Mon May 19 16:36:40 2008 Subject: [Histonet] Percent Sucrose for cryopreservation In-Reply-To: <200805191702.GSC68476@mprelay1.uc.edu> Message-ID: <771F0CF37F5F7E44884D8022D2A3EAB4031662F6@ucmail6.ad.uc.edu> Dear List: What are the considerations in choosing the percent of sucrose for cryopreservation for frozen sectioning? Here is what we are doing: We perfuse mice with 4% peraformaldehyde in 0.1M phosphate buffer, then fix overnight in the same. We dissect out either brain tissue or nose tissue. The nasal tissues are decalcified with 10% formic acid, then rinsed. All tissues are then immersed currently in 30% sucrose in 0.1M phosphate buffer. We freeze in OCT compound using dry ice. Cryostat sections are cut at 14-20 um, depending on the tissue. We then do immunostaining for a variety of antigens, including BrdU. Our question is: Is 30% sucrose optimal or should we use a lower percentage? We have heard that 30% may be too high. How would we determine that? We have not had any problems, so we are inclined of course to let it go, but perhaps we could optimize it for this next important set of experiments. Thanks, Sarah Pixley From eearle <@t> ccpathology.com Mon May 19 17:33:09 2008 From: eearle <@t> ccpathology.com (Elizabeth Earle) Date: Mon May 19 17:33:17 2008 Subject: [Histonet] White Nose Syndrome - maybe not histology related Message-ID: Hi is anyone working with or does anybody know anyone who might be working with White Nose Syndrome of bats? Thanks Elizabeth From jmjohnson34 <@t> hotmail.com Mon May 19 17:47:17 2008 From: jmjohnson34 <@t> hotmail.com (Jennifer Johnson) Date: Mon May 19 17:47:21 2008 Subject: [Histonet] Formaldehyde + Acetic Acid Message-ID: Thanks for all of your responses. I guess I didn't think this one through before sending. My Pathologist had asked me to ask all of you why formalin and acetic acid left a milky white turbitity when mixed. What he was doing was mixing FAA + Formalin which caused this reaction. Have you ever had one of those days where you stop for one second to relax and your boss sees you do this and then assumes that you have nothing to do? He then commenses to find "wild goose chases" to send you on which occupy (waste) what little time you had to do your job _________________________________________________________________ E-mail for the greater good. Join the i?m Initiative from Microsoft. http://im.live.com/Messenger/IM/Join/Default.aspx?source=EML_WL_ GreaterGood From Maria.Mejia <@t> ucsf.edu Mon May 19 17:52:16 2008 From: Maria.Mejia <@t> ucsf.edu (Mejia, Maria) Date: Mon May 19 17:52:21 2008 Subject: [Histonet] one heck of a IHC question regarding fixed free-floating brain sections Message-ID: <6CF686BD6F24A546B85B24FE3B97864701B79029@EXVS06.net.ucsf.edu> Dear All, I have one heck of a question for everyone & especially those who work with fixed 40um free-floating brain sections. A number of our very precious brain sections were mistakenly stained with the (wrong) primary (anti-human NTN) antibody. Now, we KNOW that there is NO NTN (which influences a variety of neuronal populations in the brain) in these sections. All sections (except 1) have NOT gone through the DAB development. So, we need to know [if] & [how] we can restain these sections with the correct primary antibod Here the protocol using the wrong primary antibody - please read carefully. -wash sections in PBS x3 - 5 mins ea. -block in 1% H202/PBS - 20 mins -wash sections in PBS x3 - 5 mins -block in casein biocare sniper - 30 mins -incubate in anti-goat primary NTN 1:4000 antibody in diluent - stained overnight. Next Day: -wash in PBS x3 - 5 mins ea. -incubate in Biocare Medical Goat probe - 1 hr. -wash in PBS x3 - 5 mins ea. -incubate in Biocare Medical Goat HRP - 1 hr. -wash x3 PBS - 5 mins ea. -develop ONLY ONE SECTION in Vector DAB - here's when we caught our mistake. Since we only developed 1 section in DAB - the other sections are still sitting in PBS @ 4C & have NOT gone through the DAB development. My question is can we & how - do we re-stain these undeveloped (no DAB) sections using the correct primary antibody either polyclonal or monoclonal using either the same goat probe & goat HRP or another polymer combination. Then develop the resulting end with DAB??????? If we can how do we do it???????? Please any insightful suggestions & tips will be greatly helpful to us. Please Dr. Loos let me hear from you!!! Regards Maria Mejia Histology Manager Department of Neurosurgery UCSF San Francisco, CA 94103 From Linke_Noelle <@t> Allergan.com Mon May 19 18:13:22 2008 From: Linke_Noelle <@t> Allergan.com (Linke_Noelle) Date: Mon May 19 18:17:40 2008 Subject: [Histonet] one heck of a IHC question regarding fixed free-floating brain sections In-Reply-To: <6CF686BD6F24A546B85B24FE3B97864701B79029@EXVS06.net.ucsf.edu> References: <6CF686BD6F24A546B85B24FE3B97864701B79029@EXVS06.net.ucsf.edu> Message-ID: <5C3DA4BE34AA0641BAA10A7C1478B60527D28A@IRMAIL132.irvine.allergan.com> Hi Maria, I say go back with the right antibody(don't use a goat Ab.... use rabbit or mouse), followed by anti-whatever(rabbit or mouse), and you'll need to use a Alk-phos detection and chromogen so you don't light up any of the HRP labeled stuff. Or....if you know they will be negative, I would think that doing the DAB development would simply bind up the HRP sites (if any are there), then you could start your staining all over.... Noelle No?lle Linke, MS, HTL(ASCP)QIHC Allergan, Inc 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5568 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mejia, Maria Sent: Monday, May 19, 2008 3:52 PM To: histonet@lists.utsouthwestern.edu Cc: c.m.vanderloos@amc.uva.nl Subject: [Histonet] one heck of a IHC question regarding fixed free-floating brain sections Dear All, I have one heck of a question for everyone & especially those who work with fixed 40um free-floating brain sections. A number of our very precious brain sections were mistakenly stained with the (wrong) primary (anti-human NTN) antibody. Now, we KNOW that there is NO NTN (which influences a variety of neuronal populations in the brain) in these sections. All sections (except 1) have NOT gone through the DAB development. So, we need to know [if] & [how] we can restain these sections with the correct primary antibod Here the protocol using the wrong primary antibody - please read carefully. -wash sections in PBS x3 - 5 mins ea. -block in 1% H202/PBS - 20 mins -wash sections in PBS x3 - 5 mins -block in casein biocare sniper - 30 mins -incubate in anti-goat primary NTN 1:4000 antibody in diluent - stained overnight. Next Day: -wash in PBS x3 - 5 mins ea. -incubate in Biocare Medical Goat probe - 1 hr. -wash in PBS x3 - 5 mins ea. -incubate in Biocare Medical Goat HRP - 1 hr. -wash x3 PBS - 5 mins ea. -develop ONLY ONE SECTION in Vector DAB - here's when we caught our mistake. Since we only developed 1 section in DAB - the other sections are still sitting in PBS @ 4C & have NOT gone through the DAB development. My question is can we & how - do we re-stain these undeveloped (no DAB) sections using the correct primary antibody either polyclonal or monoclonal using either the same goat probe & goat HRP or another polymer combination. Then develop the resulting end with DAB??????? If we can how do we do it???????? Please any insightful suggestions & tips will be greatly helpful to us. Please Dr. Loos let me hear from you!!! Regards Maria Mejia Histology Manager Department of Neurosurgery UCSF San Francisco, CA 94103 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From WWmn916 <@t> aol.com Mon May 19 21:31:27 2008 From: WWmn916 <@t> aol.com (WWmn916@aol.com) Date: Mon May 19 21:31:41 2008 Subject: [Histonet] Smudged inked on cassettes Message-ID: Hello histo-folks, I tried to search the archives, but couldn't come up with it.... Here's the question: We seem to be having some problems with smudged cassettes after processing. We use the Surgipath cassette printer and ribbon. Even recently, the grossing folks said the printer ribbon was brand new and we still ended up with some cassettes that completely smudged. We instruct the people who load the traditional VIP processors to not touch the numbered part of the cassettes as they load them or embedders during the embedding process. Still we get some cassettes that smudge almost unreadable. Somewhere along the way, I once heard that fatty breast tissue will cause smudging during processing. Is this a possibility? If so, how do we correct any of these problems? Thanks, Deb King **************Wondering what's for Dinner Tonight? Get new twists on family favorites at AOL Food. (http://food.aol.com/dinner-tonight?NCID=aolfod00030000000001) From anh2006 <@t> med.cornell.edu Mon May 19 22:16:16 2008 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Mon May 19 22:18:47 2008 Subject: [Histonet] one heck of a IHC question regarding fixedfree-floating brain sections In-Reply-To: <5C3DA4BE34AA0641BAA10A7C1478B60527D28A@IRMAIL132.irvine.allergan.com> References: <6CF686BD6F24A546B85B24FE3B97864701B79029@EXVS06.net.ucsf.edu><5C3DA4BE34AA0641BAA10A7C1478B60527D28A@IRMAIL132.irvine.allergan.com> Message-ID: <77784374-1211253517-cardhu_decombobulator_blackberry.rim.net-541217240-@bxe115.bisx.prod.on.blackberry> If you want to inactivate the residual hrp just incubate in hydrogen peroxiede step again. I do sequential double stains with two HRP based chromagen/substrates and do this all the time. I use 0.3% or 3% but I don't work with thick sections so you may need to tweak that a bit. I agree just redo the protocol. Using a different color might be a good idea though just so you can track any interference from your first stain. -----Original Message----- From: Linke_Noelle Date: Mon, 19 May 2008 16:13:22 To:"Mejia, Maria" Cc:histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] one heck of a IHC question regarding fixed free-floating brain sections Hi Maria, I say go back with the right antibody(don't use a goat Ab.... use rabbit or mouse), followed by anti-whatever(rabbit or mouse), and you'll need to use a Alk-phos detection and chromogen so you don't light up any of the HRP labeled stuff. Or....if you know they will be negative, I would think that doing the DAB development would simply bind up the HRP sites (if any are there), then you could start your staining all over.... Noelle No?lle Linke, MS, HTL(ASCP)QIHC Allergan, Inc 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5568 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mejia, Maria Sent: Monday, May 19, 2008 3:52 PM To: histonet@lists.utsouthwestern.edu Cc: c.m.vanderloos@amc.uva.nl Subject: [Histonet] one heck of a IHC question regarding fixed free-floating brain sections Dear All, I have one heck of a question for everyone & especially those who work with fixed 40um free-floating brain sections. A number of our very precious brain sections were mistakenly stained with the (wrong) primary (anti-human NTN) antibody. Now, we KNOW that there is NO NTN (which influences a variety of neuronal populations in the brain) in these sections. All sections (except 1) have NOT gone through the DAB development. So, we need to know [if] & [how] we can restain these sections with the correct primary antibod Here the protocol using the wrong primary antibody - please read carefully. -wash sections in PBS x3 - 5 mins ea. -block in 1% H202/PBS - 20 mins -wash sections in PBS x3 - 5 mins -block in casein biocare sniper - 30 mins -incubate in anti-goat primary NTN 1:4000 antibody in diluent - stained overnight. Next Day: -wash in PBS x3 - 5 mins ea. -incubate in Biocare Medical Goat probe - 1 hr. -wash in PBS x3 - 5 mins ea. -incubate in Biocare Medical Goat HRP - 1 hr. -wash x3 PBS - 5 mins ea. -develop ONLY ONE SECTION in Vector DAB - here's when we caught our mistake. Since we only developed 1 section in DAB - the other sections are still sitting in PBS @ 4C & have NOT gone through the DAB development. My question is can we & how - do we re-stain these undeveloped (no DAB) sections using the correct primary antibody either polyclonal or monoclonal using either the same goat probe & goat HRP or another polymer combination. Then develop the resulting end with DAB??????? If we can how do we do it???????? Please any insightful suggestions & tips will be greatly helpful to us. Please Dr. Loos let me hear from you!!! Regards Maria Mejia Histology Manager Department of Neurosurgery UCSF San Francisco, CA 94103 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nasonkini <@t> mail.nih.gov Mon May 19 22:31:19 2008 From: nasonkini <@t> mail.nih.gov (Igor Nasonkin) Date: Mon May 19 22:31:28 2008 Subject: FW: [Histonet] one heck of a IHC question regarding fixedfree-floating brain sections In-Reply-To: <77784374-1211253517-cardhu_decombobulator_blackberry.rim.net-541217240-@bxe115.bisx.prod.on.blackberry> Message-ID: Maria, you need to block the 1st primary antibody with IgG, and then use appropriate serum, before using another primary from the same species. I would use a different primary AB from a different species, i.e. if u used goat anti.. The 1st time (when mistake was made) now use rabbit anti.. If you have to use another primary from the same species - I suggest review excellent protocols on jacksonimmuno.com (labeling with 2 primaries from the same species). This works, although may give you some background if blocking is not done properly. Igor Dr. Igor O. Nasonkin Research Fellow National Institutes of Health/NEI 9000 Rockville Pike, MSC 1864 Bldg 10, Room 10B11 Bethesda, MD 20892 Tel: 301-443-7398 ?work 617-388-4104 ?cell Fax 301-480-1769 email: nasonkini@mail.nih.gov http://www.nei.nih.gov/intramural/nnrl.asp ------ Forwarded Message > From: > Reply-To: > Date: Tue, 20 May 2008 03:16:16 +0000 > To: Linke_Noelle , > , "Mejia, Maria" > > Cc: > Subject: Re: [Histonet] one heck of a IHC question regarding > fixedfree-floating brain sections > > histonet@lists.utsouthwestern.edu ------ End of Forwarded Message From anthony <@t> histotechexchange.com Mon May 19 22:18:42 2008 From: anthony <@t> histotechexchange.com (anthony@histotechexchange.com) Date: Mon May 19 22:48:20 2008 Subject: [Histonet] Microwave deparaffinization. In-Reply-To: <549014.59137.qm@web65710.mail.ac4.yahoo.com> References: <549014.59137.qm@web65710.mail.ac4.yahoo.com> Message-ID: <1443.65.40.219.182.1211253522.squirrel@host7.wfdns.com> Dear all: Paraffin can be heated by a microwave. Normal microwaves are set to create a wavelength that affects water molecules. You need a microwave that affects long carbon chain molecules. Funny, I was talking to a chemist who teaches at our local University, and I asked her about breaking down wax less complex carbon chain molecules. She told me that you can but. Unfortunately, when not tightly regulated, it is called a fire. Talk about being flamed. Ha ha. Yours truly, Anthony Williams BSc. HT (ASCP) Histotech Exchange LLC 19 Whitmore St. Lexington, VA 24450 T 1 877 464 8911 T 1 877 GO HT 911 F 1 540 301 0071 anthony@histotechexchange.com www.histotechexchange.com Paraffin is "transparent" or "translucent" to microwaves, meaning that > paraffin CANNOT be melted or even heated using microwaves. > Instruments using microwaves heat the container (made of or including > parts made of WEFLON) that melt the paraffin by convection from the hot > container but NOT because the paraffin is directly heated. > This is one of those good ideas that will not fly! > Ren? J. > > Sebasti?n Vecchio wrote: > I have been trying to deparafinize section in the lab, but I cannot get > the > right time and power for the MW oven, does any one has a protocol for this > . > This method eliminates the uses of xylol in the processing of staining > sections. > Thanks. > Sebastian, Student of Histotechnology, EUTM, FMED, UDELAR. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From annigyg <@t> gmail.com Mon May 19 22:56:32 2008 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Mon May 19 22:56:36 2008 Subject: [Histonet] Formaldehyde + Acetic Acid In-Reply-To: References: Message-ID: my day is a series of 'wild goose chases' the latest one is to uncoverslip (glass+DPX), destain and restain several BOXES of teaching slides from the early '60s ....but no coverslip-tape thank you very much!!! ha ha ha!!! I'm ready for the funny farm Annie 2008/5/20 Jennifer Johnson : > > Thanks for all of your responses. I guess I didn't think this one through > before sending. My Pathologist had asked me to ask all of you why formalin > and acetic acid left a milky white turbitity when mixed. What he was doing > was mixing FAA + Formalin which caused this reaction. Have you ever had one > of those days where you stop for one second to relax and your boss sees you > do this and then assumes that you have nothing to do? He then commenses to > find "wild goose chases" to send you on which occupy (waste) what little > time you had to do your job > _________________________________________________________________ > E-mail for the greater good. Join the i'm Initiative from Microsoft. > http://im.live.com/Messenger/IM/Join/Default.aspx?source=EML_WL_GreaterGood_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From anh2006 <@t> med.cornell.edu Mon May 19 23:13:18 2008 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Mon May 19 23:13:16 2008 Subject: FW: [Histonet] one heck of a IHC question regarding fixedfree-floating brain sections In-Reply-To: References: Message-ID: Yes sorry my message was assuming your primaries were from different species, otherwise indeed you need to use specialized blocking methods ... I think I am too tired to be emailing clearly!!! >Maria, > you need to block the 1st primary antibody with IgG, and then use >appropriate serum, before using another primary from the same species. >I would use a different primary AB from a different species, i.e. if u used >goat anti.. The 1st time (when mistake was made) now use rabbit anti.. > >If you have to use another primary from the same species - I suggest review >excellent protocols on jacksonimmuno.com (labeling with 2 primaries from the >same species). This works, although may give you some background if blocking >is not done properly. > >Igor > >Dr. Igor O. Nasonkin >Research Fellow >National Institutes of Health/NEI >9000 Rockville Pike, MSC 1864 >Bldg 10, Room 10B11 >Bethesda, MD 20892 >Tel: 301-443-7398 -work > 617-388-4104 -cell >Fax 301-480-1769 >email: nasonkini@mail.nih.gov >http://www.nei.nih.gov/intramural/nnrl.asp > > >------ Forwarded Message >> From: >> Reply-To: >> Date: Tue, 20 May 2008 03:16:16 +0000 >> To: Linke_Noelle , >> , "Mejia, Maria" >> >> Cc: >> Subject: Re: [Histonet] one heck of a IHC question regarding >> fixedfree-floating brain sections >> >> histonet@lists.utsouthwestern.edu > >------ End of Forwarded Message > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- From sbreeden <@t> nmda.nmsu.edu Tue May 20 07:12:02 2008 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue May 20 07:12:10 2008 Subject: [Histonet] Lab temperatures Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E630C@nmdamailsvr.nmda.ad.nmsu.edu> For the first 2 years I worked here, the summertime temperature in the lab averaged 83F. My lab is approximately 12x15' and all my equipment is in here (processor, stainer, oven, flotation bath, Ventana, embedding unit, refrigerator). Can you say "hot"? I complained (sweetly) to Maintenance, who explained that VDS was at the end of the AC plenum and that there was really nothing they could do. The beginning of my 3rd year, I complained (ever so sweetly again) that if the temperature did not improve, I was going to work naked. Apparently they figured this would not be a good thing, and miraculously a new thermostat solved the problem in my lab. As I write, it is 72.5F and 22% humidity. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From sherylmccandless <@t> grandecom.net Tue May 20 07:38:30 2008 From: sherylmccandless <@t> grandecom.net (Sheryl McCandless) Date: Tue May 20 07:38:37 2008 Subject: [Histonet] Re: Salary Scales Message-ID: I agree that the pay is not so great, but I think the lack of respect and the "anyone can do it attitude" probably makes the low pay worse. After I got my certification, I couldn't even get market value for my area, but what bothered me the most was that I was treated like the certification was "no big deal" and it really didn't seem to matter whether I was certified or not. I could easily be replaced with anyone. That's really a shame when you think about the patient care aspect. A lab is entrusted with their specimens and their lives, yet some pathologists don't care whether their techs took their job seriously enough to do the work to get certified. I suppose they care more about their pocketbook. As much as I enjoyed working in histology, it was enough to make me leave the field at least for now. Maybe one day histotechs will not only get better pay, but also a thank you every now and then. From rjbuesa <@t> yahoo.com Tue May 20 07:44:07 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue May 20 07:44:14 2008 Subject: [Histonet] Formalin+Acetic Acid In-Reply-To: Message-ID: <52608.66450.qm@web65716.mail.ac4.yahoo.com> There is nothing that can cause a milky appearance due to mixing formaldehyde and acetic acid. You must have a contaminant or another substance in that mix. By themselves formaldehyde + acetic acid do not produce any color or precipitate. Ren? J. Jennifer Johnson wrote: Does anyone know of the top of his/her head what the chemical reaction is between formaldehyde and acetic acid that causes the milky white turbitity? Thanks, Jennifer _________________________________________________________________ Keep your kids safer online with Windows Live Family Safety. http://www.windowslive.com/family_safety/overview.html?ocid=TXT_TAGLM_WL_Refresh_family_safety_052008_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue May 20 07:57:47 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue May 20 07:57:54 2008 Subject: [Histonet] Formaldehyde + Acetic Acid In-Reply-To: Message-ID: <383417.87568.qm@web65703.mail.ac4.yahoo.com> Not even that: FAA (formaldehyde + alcohol + acetic acid) + formalin do NOT produce any milky solution. Tell you PT to check what s/he was mixing, that had to be something else in that mixture. Ren? J. Jennifer Johnson wrote: From rjbuesa <@t> yahoo.com Tue May 20 08:16:34 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue May 20 08:16:38 2008 Subject: [Histonet] Microwave deparaffinization. In-Reply-To: <1443.65.40.219.182.1211253522.squirrel@host7.wfdns.com> Message-ID: <140104.79203.qm@web65701.mail.ac4.yahoo.com> Anthony: It is not necessary to start a fire to break down large complex hydrocarbon molecules. That is what the petroleum refineries do every day and is called "cracking". Paraffin cannot be heated in a MW oven. They are non polar molecules and non polar molecules are not affected by the microwaves because they cannot "vibrate" under their influence and therefore cannot transform the movement (vibration) energy induced by the MW into heat energy. Ren? J. anthony@histotechexchange.com wrote: > From JHAPPEL <@t> PARTNERS.ORG Tue May 20 08:40:52 2008 From: JHAPPEL <@t> PARTNERS.ORG (Happel, James F.) Date: Tue May 20 08:40:57 2008 Subject: [Histonet] Brightly Staining Goblet Cells in GI bx's Message-ID: Good Morning, We are finding recently that our small GI biopsies are demonstrating brightly staining goblet cells, so much so that the GI pathologists are calling it "distracting". The rest of the staining on the slide appears normal. The goblet cells are presenting a hyper-hematoxylin stippling. We've recently changed hematoxylin (we are using Richard-Allen Hematoxylin I now) however, this artifact was present prior to switching, although it is more intense now. Any ideas as to what might be causing it? Thanks! James Happel Mass General Hospital The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From rjbuesa <@t> yahoo.com Tue May 20 09:42:56 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue May 20 09:43:00 2008 Subject: [Histonet] Brightly Staining Goblet Cells in GI bx's In-Reply-To: Message-ID: <507950.64401.qm@web65704.mail.ac4.yahoo.com> It is the hematoxylin, no doubt about it. This is a consequence of the elimination of mercury oxide from Harris formula. Try weakening the hematoxylin (8:10 dilution rate). Ren? J. "Happel, James F." wrote: From ploykasek <@t> phenopath.com Tue May 20 09:44:18 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue May 20 09:44:29 2008 Subject: [Histonet] Varicella zoster In-Reply-To: <197CD0B02A81F94994A285C59C8AE05C027A94C3F8@pgnexchange.pathgroup.com> Message-ID: Dear Mighnon, we purchase a varicella zoster antibody clone M1 from Chemicon. We haven't ordered it in over a year, but I am assuming it is still available from Chemicon. It does work on formalin fixed paraffin embedded tissue using a heat retrieval. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Does anyone know where I can get an antibody for varicella zoster? Thanks, > > > Mighnon Lashus, HT (ASCP) > > PathGroup Lab > > 4071 S. Access Road, Suite 107 > > Chattanooga, TN 37406 > > 423-493-0207 > > 423-493-0208 fax > > mlashus@pathgroup.com > > > ________________________________ > Important Notice: This e-mail is intended for the use of the person to whom it > is addressed and may contain information that is privileged and confidential. > If you are not the intended recipient, any disclosure, copying, distribution, > or use of the contents of this message is strictly prohibited. If you have > received this e-mail in error, please destroy this message and contact the > Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From jkiernan <@t> uwo.ca Tue May 20 09:48:26 2008 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue May 20 09:48:34 2008 Subject: [Histonet] Percent Sucrose for cryopreservation Message-ID: According to an EM and X-ray diffraction stuudy (J.Lepault et al 1997; J. Microsc. 187: 158-166) you need 60% sucrose to prevent ice crystal formation. Most people use 20-30% sucrose, and combined with rapid freezing this is OK for light microscopy. I guess the ice crystals are too small to make visible holes in the tissue. Is there any point dissolving the sucrose in phosphate buffer rather than water? ----- Original Message ----- From: "Pixley, Sarah (pixleysk)" Date: Monday, May 19, 2008 17:37 Subject: [Histonet] Percent Sucrose for cryopreservation To: histonet@lists.utsouthwestern.edu > Dear List: > > What are the considerations in choosing the percent of sucrose for > cryopreservation for frozen sectioning? Here is what we are doing: > We perfuse mice with 4% peraformaldehyde in 0.1M phosphate > buffer, then > fix overnight in the same. We dissect out either brain tissue or nose > tissue. The nasal tissues are decalcified with 10% formic acid, then > rinsed. All tissues are then immersed currently in 30% sucrose > in 0.1M > phosphate buffer. We freeze in OCT compound using dry ice. Cryostat > sections are cut at 14-20 um, depending on the tissue. We then do > immunostaining for a variety of antigens, including BrdU. > > Our question is: Is 30% sucrose optimal or should we use a lower > percentage? We have heard that 30% may be too high. How would we > determine that? We have not had any problems, so we are > inclined of > course to let it go, but perhaps we could optimize it for this next > important set of experiments. > > Thanks, > Sarah Pixley > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mickie25 <@t> netzero.net Tue May 20 11:15:54 2008 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Tue May 20 11:16:14 2008 Subject: [Histonet] Mohs & Derm Histology Job Opening In-Reply-To: <007d01c8b6b8$b7ce7de0$6401a8c0@Patsyoffice> References: <623891.86971.qm@web65712.mail.ac4.yahoo.com><2842DC75AE43AA4B92954CFB31781BC1821654@CHW-MSG-301.chw.edu> <007d01c8b6b8$b7ce7de0$6401a8c0@Patsyoffice> Message-ID: Dear Histonetters, There is a full time job opening for a person with experience in Mohs who can also perform routine histology on dermatological specimens. This job is in New Mexico and some training is available. This opening is available now. The work environment is pleasant and busy. This is the perfect job for someone who likes variety in their work week and likes to be in charge of their lab. If you are interested, please call me at: 509-954-7134 or email me at: mickie25@netzero.net. Thank you! Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Thursday, May 15, 2008 11:23 AM To: 'Heckford, Karen - SMMC-SF'; 'Dawson, Glen'; 'Histonet (E-mail)' Subject: RE: [Histonet] Salary Scales In Colorado it depends on if you are permanently employed or working per diem, The daily people who cover and get no benefits make at least $25 per hour, but those employed with benefits go from $15 up an hour. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Thursday, May 15, 2008 11:32 AM To: Dawson, Glen; Histonet (E-mail) Subject: RE: [Histonet] Salary Scales I guess it does really depend on the area. In San Francisco it is high twenties to mid thirties. One hour north of San Francisco it can be as low as $7-10.00 an hour less. Big jump for 70 miles. Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathology Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Thursday, May 15, 2008 8:00 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Salary Scales $26.20 / hour is "decent"? For the Milwaukee area, that is a phenominal payrate. What regions see this as "run of the mill"? I wish I could offer techs this pay. It would certainly solve all of our staffing issues in one fell swoop. I also wonder where payscales like this are when the Histology pay scales are being compiled for publication. You look at our printed ranges and you cannot help but think that they are more useful to those who want to keep histo-pay low. Call me a coward but I don't feel comfortable quoting our average histotech pay. I'll just say that it is SIGNIFICANTLY below $26.20. Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Wednesday, May 14, 2008 2:22 PM To: JR R; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Salary Scales Which is equivalent to $26.20 / hour (2080 hours / year) and could be considered as "decent". Ren? J. JR R wrote: _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Erin.Martin <@t> ucsf.edu Tue May 20 11:24:37 2008 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Tue May 20 11:26:27 2008 Subject: [Histonet] Research at home Message-ID: Hi, a few days ago someone posted about cutting blocks at home and I didn't see much response. Could the person who posted please email me and tell me if you got any answers? I'm curious...Thanks! From laurie.colbert <@t> huntingtonhospital.com Tue May 20 11:53:45 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Tue May 20 11:53:50 2008 Subject: [Histonet] Job Opportunity - Pasadena, CA Message-ID: <57BE698966D5C54EAE8612E8941D768302F248CA@EXCHANGE3.huntingtonhospital.com> Huntington Memorial Hospital in Pasadena, CA has a full time histotech position available. The hours are 4:00 am - 12:30 pm, Monday - Friday. Please email me or call me directly if you are interested and would like more information. Laurie Colbert Huntington Hospital Pasadena, CA (626) 397-8620 (626) 397-2187 fax From jcline <@t> wchsys.org Tue May 20 12:00:57 2008 From: jcline <@t> wchsys.org (Joyce Cline) Date: Tue May 20 12:01:05 2008 Subject: Michelle Coker RE: [Histonet] Histoscreen Cassettes In-Reply-To: <163239.63792.qm@web90303.mail.mud.yahoo.com> Message-ID: <003401c8ba9b$13fb1b60$1d2a14ac@wchsys.org> I would like to know what kind of problems you had with the screened cassettes? Also, what kind of microwave processor do you use? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shelly Coker Sent: Friday, May 16, 2008 6:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histoscreen Cassettes Hi everyone! I have some Histoscreen cassettes I would like to dispose of. These cassettes have been in our storage for close to a year. The colors I have are blue and lilac. We currently process our specimens in a microwave processor, and these cassettes wreak havoc on our tissue. If there is anyone interested, please contact me off-list. Thanks, Michelle Coker HT(ASCP)cm _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From Terri.Brown <@t> Northside.com Tue May 20 12:03:18 2008 From: Terri.Brown <@t> Northside.com (Terri Brown) Date: Tue May 20 12:03:49 2008 Subject: [Histonet] Job Opportunity - Pasadena, CA In-Reply-To: <57BE698966D5C54EAE8612E8941D768302F248CA@EXCHANGE3.huntingtonhospital.com> References: <57BE698966D5C54EAE8612E8941D768302F248CA@EXCHANGE3.huntingtonhospital.com> Message-ID: <8CEB6DA1A3F35743800669D4CFE21F7D03F8FFD2@NSMXMS04.northside.local> Northside Hospital Atlanta, Ga has a full time histotech position available. The hours are 5 am - 1:30 pm or 6 am - 2:30 pm. We also, have a histotech position available 10:30 am - 7 pm to help cover frozens and some Immuno staining. Please e mail or call me directly if you are interested and would like more information. Terri H. Brown, HT (ASCP) Pathology Laboratory Manager Northside Hospital Office: 404-845-5423 Fax: 404-851-6400 terri.brown@northside.com CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. From joelleweaver <@t> hotmail.com Tue May 20 12:06:00 2008 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Tue May 20 12:06:04 2008 Subject: [Histonet] histotech pay/respect Message-ID: In response to several postings regarding pay and respect- I have been feeling somewhat better to read that others are disheartened by the lack of respect for the effort and time that it can take to become a certified, experienced histotech. The pay doesn't really bother me as much as the attitude that I encounter on a daily basis that "anyone" can do histology and that it is to quote "no big deal" to get certified. I have to concur that in my experience, histology managers do not seem to value or even recognize the skills and time it takes to perfect this trade.I know for me, getting an HTL was quite a burden at times. I had extra studies for sure. And, back in that day, the ASCP slide practical was no picnic. At my own hospital, this attitude is epitomized by the fact that routinely non-certifed, non-histology people are both hired and promoted. I have never seen anyone with a histology background given any sort of professional respect like that given freely to the MT personnel that work there. They do not even recgonize an HTL as a certification. They only give you a small increase for being certified at all (HT). At particularly bad moments, this had made me want to leave the field as well. So, I can relate very easily to everyone else's sentiments. This hospital has been an especially bad example of the "warm body" syndrome. It is almost crippling in terms of the quality of the work and TAT. The inferior service and poor quality produced only serves to reinforce the negative concept- it is indeed a vicious cycle! At least (all of us) are not alone! Joelle _________________________________________________________________ Keep your kids safer online with Windows Live Family Safety. http://www.windowslive.com/family_safety/overview.html?ocid=TXT_TAGLM_WL_Refresh_family_safety_052008 From KCOLP <@t> capefearvalley.com Tue May 20 12:11:31 2008 From: KCOLP <@t> capefearvalley.com (Kelly Colpitts) Date: Tue May 20 12:11:38 2008 Subject: [Histonet] Monoclonal and Polyclonal Negative Antibody Message-ID: <545926051D01CF4587A6DEF9A1750CE4015C3DBE@ntmessaging.capefear.local> To Whom It May Concern, Recently our lab was told that we would combine our pre-made Monoclonal Negative Antibody and our pre-made Polyclonal Negative Antibody in the same dispenser to run as the negative reagent on all cases. Would it be ok to mix a Mouse Monoclonal and a Rabbit Polyclonal or is the difference in species going to cause a problem? Thanks for your help! This email and any files transmitted with it are intended solely for the use of the individual or entity to whom they are addressed. The individual sender of this email is responsible for its content, and Cape Fear Valley Health System does not assume any legal liability or responsibility for the accuracy, completeness or usefulness of any information, product, or process disclosed. Further, the views and opinions of the author expressed herein do not necessarily state or reflect that of the Cape Fear Valley Health System, its management or its trustees. From TJJ <@t> Stowers-Institute.org Tue May 20 12:18:15 2008 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Tue May 20 12:18:41 2008 Subject: [Histonet] Re: one heck of a IHC question regarding fixed Message-ID: Maria - you can elute your antibody reactions using 0.1M glycine buffer pH 2.0 (5-10 minutes each, x 2 times for slide mounted tissues, perhaps a bit longer for the free floating sections). This should elute your first primary antibody from the original antigenic site. Rinse well and place in buffer to bring the pH back to near neutral. Had these been slide mounted sections, you can also successfully elute antibodies using heat induced antigen retrieval (Lan et al. JHC 43:97-102, 1985). These treatments only really work well if you know it will not compromise the staining with the subsequent antibody. Otherwise, if I understand correctly, your correct antibody is also made in goat. If that is the case, then use the goat serum block or (my preferred method) is the anti-goat FAB fragments from Jackson Immunochemicals (previously recommended), and then proceed with the correct immunostaining protocol. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From GDawson <@t> dynacaremilwaukee.com Tue May 20 13:45:16 2008 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Tue May 20 13:45:25 2008 Subject: [Histonet] histotech pay/respect In-Reply-To: Message-ID: Joelle, Best pathologist quote that I've ever heard myself: "Let's face it, a trained monkey can cut blocks." This was referring to trying to staff his personal research lab and was said with all seriousness. Needless to say, this pathologist and I didn't get along well at all. Unfortunately, too many share these sentiments and don't have a clue that a histotech tends to do a bit more than "cut blocks". Sort of makes you wonder why any of us bothers with getting certified or with obtaining necessary CEU's to stay that way. Hang in There, Glen Dawson BS, HT & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of joelle weaver Sent: Tuesday, May 20, 2008 12:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] histotech pay/respect In response to several postings regarding pay and respect- I have been feeling somewhat better to read that others are disheartened by the lack of respect for the effort and time that it can take to become a certified, experienced histotech. The pay doesn't really bother me as much as the attitude that I encounter on a daily basis that "anyone" can do histology and that it is to quote "no big deal" to get certified. I have to concur that in my experience, histology managers do not seem to value or even recognize the skills and time it takes to perfect this trade.I know for me, getting an HTL was quite a burden at times. I had extra studies for sure. And, back in that day, the ASCP slide practical was no picnic. At my own hospital, this attitude is epitomized by the fact that routinely non-certifed, non-histology people are both hired and promoted. I have never seen anyone with a histology background given any sort of professional respect like that given freely to the MT personnel that work there. They do not even recgonize an HTL as a certification. They only give you a small increase for being certified at all (HT). At particularly bad moments, this had made me want to leave the field as well. So, I can relate very easily to everyone else's sentiments. This hospital has been an especially bad example of the "warm body" syndrome. It is almost crippling in terms of the quality of the work and TAT. The inferior service and poor quality produced only serves to reinforce the negative concept- it is indeed a vicious cycle! At least (all of us) are not alone! Joelle _________________________________________________________________ Keep your kids safer online with Windows Live Family Safety. http://www.windowslive.com/family_safety/overview.html?ocid=TXT_TAGLM_WL_Refresh_family_safety_052008_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmmathis1 <@t> bellsouth.net Tue May 20 13:52:36 2008 From: cmmathis1 <@t> bellsouth.net (cmmathis1@bellsouth.net) Date: Tue May 20 13:52:42 2008 Subject: [Histonet] Sample antibodies Message-ID: <052020081852.19529.48331DF400087B3200004C4922218675169B0A02D2089B9A019C04040A0DBFCE9C07089B0E03030C@att.net> Are there any companies that have sample antibodies or any kind of trial size? Cathy Mathis From LSebree <@t> uwhealth.org Tue May 20 14:11:19 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Tue May 20 14:11:24 2008 Subject: [Histonet] Sample antibodies In-Reply-To: <052020081852.19529.48331DF400087B3200004C4922218675169B0A02D2089B9A019C04040A0DBFCE9C07089B0E03030C@att.net> Message-ID: NeoMarkers sells 0.1 ml sizes. These are marketed through the Lab Vision division of Thermo Scientific, phone #: 1(800)828-1628. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of cmmathis1@bellsouth.net Sent: Tuesday, May 20, 2008 1:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sample antibodies Are there any companies that have sample antibodies or any kind of trial size? Cathy Mathis _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DixonM <@t> vetmed.ufl.edu Tue May 20 14:13:20 2008 From: DixonM <@t> vetmed.ufl.edu (MaryAnn Dixon) Date: Tue May 20 14:13:47 2008 Subject: [Histonet] NSH bulletin (In Action) In-Reply-To: <052020081852.19529.48331DF400087B3200004C4922218675169B0A02D2089B9A019C04040A0DBFCE9C07089B0E03030C@att.net> References: <052020081852.19529.48331DF400087B3200004C4922218675169B0A02D2089B9A019C04040A0DBFCE9C07089B0E03030C@att.net> Message-ID: <4832EA8F.6EA2.00FD.0@vetmed.ufl.edu> Hi Histonetters, Has anyone read the In Action NSH bulletin lately? Just another example of the lack of recognition for the field of Histology. I am new to the field of Histology. What has happened to those employed in the NY labs? Are other states following by example? Should I get out now before I am unemployed? MaryAnn Dixon Biological Scientist Anatomic Pathology University of Florida Medical Center 352-392-2235 ext 4517 From ploykasek <@t> phenopath.com Tue May 20 14:13:58 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue May 20 14:14:08 2008 Subject: [Histonet] histotech pay/respect In-Reply-To: Message-ID: It is sad to think that there are so many negative experiences out there. I feel lucky that I have never experienced this. Most places I have worked the pathologists considered us part of a team in patient care. That's not to say I never heard disrespectful remarks, but for the most part have always been treated well. At the last hospital I worked in Tulsa, the med techs even encouraged me to ask for more money when I obtained my specialty QIHC certification, as the med techs there received more when they obtained specialty certification. I will say that I always try to treat all staff I encounter - Physicians & all other lab techs, etc- with respect & an attitude of "how can I help you". We're all in this to provide good patient care. I have enjoyed all the various places I have worked in my career. I didn't realize exactly how lucky I have been. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > > In response to several postings regarding pay and respect- I have been feeling > somewhat better to read that others are disheartened by the lack of respect > for the effort and time that it can take to become a certified, experienced > histotech. The pay doesn't really bother me as much as the attitude that I > encounter on a daily basis that "anyone" can do histology and that it is to > quote "no big deal" to get certified. I have to concur that in my experience, > histology managers do not seem to value or even recognize the skills and time > it takes to perfect this trade.I know for me, getting an HTL was quite a > burden at times. I had extra studies for sure. And, back in that day, the ASCP > slide practical was no picnic. At my own hospital, this attitude is epitomized > by the fact that routinely non-certifed, non-histology people are both hired > and promoted. I have never seen anyone with a histology background given any > sort of professional respect like that given freely to the MT personnel that > work there. They do not even recgonize an HTL as a certification. They only > give you a small increase for being certified at all (HT). At particularly bad > moments, this had made me want to leave the field as well. So, I can relate > very easily to everyone else's sentiments. This hospital has been an > especially bad example of the "warm body" syndrome. It is almost crippling in > terms of the quality of the work and TAT. The inferior service and poor > quality produced only serves to reinforce the negative concept- it is indeed a > vicious cycle! > At least (all of us) are not alone! > Joelle > _________________________________________________________________ > Keep your kids safer online with Windows Live Family Safety. > http://www.windowslive.com/family_safety/overview.html?ocid=TXT_TAGLM_WL_Refre > sh_family_safety_052008_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From pdunlop720 <@t> gmail.com Tue May 20 14:20:17 2008 From: pdunlop720 <@t> gmail.com (Patty Dunlop) Date: Tue May 20 14:20:22 2008 Subject: [Histonet] xylene substitute recoverslipping Message-ID: <80ab7bc60805201220h44cb0e54y8223f5231a7fc495@mail.gmail.com> Hello Histonetters, I use Clear-Rite 3, a xylene substitute, and am having problems when I need to recoverslip. Currently, I just put the slide that needs recoverslipped into a coplin jar of Clear-rite 3 at room temperature. The coverslip really doesn't like to come off (takes a week) and it tends to be a sticky, messy process. Does anyone have a method for removing coverslips with a xylene substitute? Should I use heat? Thanks, Patty From lblazek <@t> digestivespecialists.com Tue May 20 14:35:25 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue May 20 14:30:08 2008 Subject: [Histonet] histotech pay/respect In-Reply-To: References: Message-ID: <1F937FB30BDB7C4A9F39F83FEA8D379F9F54DB@bruexchange1.digestivespecialists.com> Patti and all, Horary Patti. I was just sitting here getting ready to post my email when you posted yours. I was beginning to think I was the only one here that has had a very positive experience as a histotech. I've been watching this topic for a long time now and wonder where I've been. I've been a histotech since the early 70's. The pathologist I worked for in the early 80's wanted to know why I never took my registry when I finished school. I guess a baby got in the way and no one really required it. He pushed and encouraged me to take my registry. He was so supportive and helpful to someone that waited 9 years to sit for the exam that it was amazing. I did receive a healthy raise when I passed. I have never in all these years worked for a pathologist that wasn't respectful and included me as a part of a team. I have had a bit of "red-headed stepchild" reactions from MTs but that never bothered me since none of them had a clue as to what a histotech did. When they removed the histology rotation from the MT training in the early 70's they didn't have any exposure to our world. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Tuesday, May 20, 2008 2:45 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect Joelle, Best pathologist quote that I've ever heard myself: "Let's face it, a trained monkey can cut blocks." This was referring to trying to staff his personal research lab and was said with all seriousness. Needless to say, this pathologist and I didn't get along well at all. Unfortunately, too many share these sentiments and don't have a clue that a histotech tends to do a bit more than "cut blocks". Sort of makes you wonder why any of us bothers with getting certified or with obtaining necessary CEU's to stay that way. Hang in There, Glen Dawson BS, HT & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of joelle weaver Sent: Tuesday, May 20, 2008 12:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] histotech pay/respect In response to several postings regarding pay and respect- I have been feeling somewhat better to read that others are disheartened by the lack of respect for the effort and time that it can take to become a certified, experienced histotech. The pay doesn't really bother me as much as the attitude that I encounter on a daily basis that "anyone" can do histology and that it is to quote "no big deal" to get certified. I have to concur that in my experience, histology managers do not seem to value or even recognize the skills and time it takes to perfect this trade.I know for me, getting an HTL was quite a burden at times. I had extra studies for sure. And, back in that day, the ASCP slide practical was no picnic. At my own hospital, this attitude is epitomized by the fact that routinely non-certifed, non-histology people are both hired and promoted. I have never seen anyone with a histology background given any sort of professional respect like that given freely to the MT personnel that work there. They do not even recgonize an HTL as a certification. They only give you a small increase for being certified at all (HT). At particularly bad moments, this had made me want to leave the field as well. So, I can relate very easily to everyone else's sentiments. This hospital has been an especially bad example of the "warm body" syndrome. It is almost crippling in terms of the quality of the work and TAT. The inferior service and poor quality produced only serves to reinforce the negative concept- it is indeed a vicious cycle! At least (all of us) are not alone! Joelle _________________________________________________________________ Keep your kids safer online with Windows Live Family Safety. http://www.windowslive.com/family_safety/overview.html?ocid=TXT_TAGLM_WL _Refresh_family_safety_052008___________________________________________ ____ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From september.amspacher <@t> bassett.org Tue May 20 14:31:03 2008 From: september.amspacher <@t> bassett.org (Amspacher, September) Date: Tue May 20 14:33:54 2008 Subject: [Histonet] NSH bulletin (In Action) In-Reply-To: <4832EA8F.6EA2.00FD.0@vetmed.ufl.edu> References: <052020081852.19529.48331DF400087B3200004C4922218675169B0A02D2089B9A019C04040A0DBFCE9C07089B0E03030C@att.net>, <4832EA8F.6EA2.00FD.0@vetmed.ufl.edu> Message-ID: MaryAnn- Please do not leave the field-I think I can speak for everyone there are days but I love my job, I love doing histology and I would hate to see anyone give it up. We of New York state are and having been fighting hard. While we were very excited to see the article and see what NSH was doing on our behalf, It did not have the latest update. There is an Amendment to the legislation that is currently in both the NYS Senate and NYS Assembly and we have our fingers crossed that it will go through before the end of the current session which is the end of June. We have some very powerful political people backing our efforts and with everything in place we are hopeful of once again being Histotechs and not med techs- It has been a huge battle and one that has affected all of us, from some of us (myself included) moving back to New York and being told that your professional degree and training is no longer recognized and each and every one of us paying the $250-$295 for the right to work in the state of New York. Other states for example Florida, Rhode Island and Nevada have state licensure but if you have taken and passed your ASCP exam you are grandfathered in after paying the fees ( Note Rhode Island is $32.50 and Nevada is $75.00 ). Thank you for your concern for those of us working in New York State. Keep your chin up and good luck in the field- I hope you enjoy it just as much as I do. September Amspacher HT(ASCP) Sr. Tech/ Histologist Bassett Healthcare, Cooperstown NY From: MaryAnn Dixon Sent: Tue 5/20/2008 3:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NSH bulletin (In Action) Hi Histonetters, Has anyone read the In Action NSH bulletin lately? Just another example of the lack of recognition for the field of Histology. I am new to the field of Histology. What has happened to those employed in the NY labs? Are other states following by example? Should I get out now before I am unemployed? MaryAnn Dixon Biological Scientist Anatomic Pathology University of Florida Medical Center 352-392-2235 ext 4517 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE OF CONFIDENTIALITY This electronic message, including attachments, is for the sole use of the named recipient and may contain confidential or privileged information protected by New York State, and Federal regulations. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. If you are not the intended recipient or have received this communication in error please contact the sender or email.security@bassett.org and destroy all copies of the original message. Thank you. From gayle.callis <@t> bresnan.net Tue May 20 15:00:40 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Tue May 20 15:00:38 2008 Subject: [Histonet] xylene substitute recoverslipping References: <80ab7bc60805201220h44cb0e54y8223f5231a7fc495@mail.gmail.com> Message-ID: <007701c8bab4$2e2d27d0$6501a8c0@DHXTS541> We use xylene to remove coverslips and do this work inside a hood. After coverslip is off and mounting media is gone with an extra xylene rinse, we go back to Clearite 3 to rinse off xylene and recoverslip. Anything to try an minimize exposure to xylene is done. Unfortunately, we found the substitute just didn't work for this job. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT ----- Original Message ----- From: "Patty Dunlop" To: Sent: Tuesday, May 20, 2008 1:20 PM Subject: [Histonet] xylene substitute recoverslipping > Hello Histonetters, > > I use Clear-Rite 3, a xylene substitute, and am having problems when I > need > to recoverslip. Currently, I just put the slide that needs recoverslipped > into a coplin jar of Clear-rite 3 at room temperature. The coverslip > really > doesn't like to come off (takes a week) and it tends to be a sticky, messy > process. Does anyone have a method for removing coverslips with a xylene > substitute? Should I use heat? > > Thanks, > Patty > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JGREWE <@t> OhioHealth.com Tue May 20 15:01:35 2008 From: JGREWE <@t> OhioHealth.com (JGREWE@OhioHealth.com) Date: Tue May 20 15:01:44 2008 Subject: [Histonet] Jacquelyn Grewe/Staff/OhioHealth is out of the office . Message-ID: I will be out of the office starting 05/20/2008 and will not return until 06/02/2008. From brett_connolly <@t> merck.com Tue May 20 15:03:22 2008 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Tue May 20 15:03:33 2008 Subject: [Histonet] CD11b on FFPE mouse sections Message-ID: <63EA0607835FBA4689CEA9EA8B48269201166D54@usctmx1141.merck.com> Looking for recommendations for anti- CD11b on paraffin mouse sections? I see Serotec has one...anyone have experience with it? or others? Thanks, Brett Brett M. Connolly, Ph.D. Research Fellow MRL, Imaging Research Merck & Co., Inc. WP-44K PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From slappycraw <@t> yahoo.com Tue May 20 15:14:45 2008 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Tue May 20 15:14:52 2008 Subject: [Histonet] histotech pay/respect In-Reply-To: Message-ID: <520971.93996.qm@web53611.mail.re2.yahoo.com> I have worked on both sides of this issue and in my opinion if you're not getting the respect or the pay that you think you deserve then if at all possible try to find another place to go. I know for some it's not really possible to pick up and move but if you can it might be your best option. It's too bad we don't have a list of the 100 best pathology laboratories to work in. If places have good people constantly leaving you'd think eventually someone would get the message. Sometimes it just takes a couple good people in the right places to turn things around. I know wherever I have lived and worked, the word got around pretty quickly on the best places to work. Patti Loykasek wrote: It is sad to think that there are so many negative experiences out there. I feel lucky that I have never experienced this. Most places I have worked the pathologists considered us part of a team in patient care. That's not to say I never heard disrespectful remarks, but for the most part have always been treated well. At the last hospital I worked in Tulsa, the med techs even encouraged me to ask for more money when I obtained my specialty QIHC certification, as the med techs there received more when they obtained specialty certification. I will say that I always try to treat all staff I encounter - Physicians & all other lab techs, etc- with respect & an attitude of "how can I help you". We're all in this to provide good patient care. I have enjoyed all the various places I have worked in my career. I didn't realize exactly how lucky I have been. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > > In response to several postings regarding pay and respect- I have been feeling > somewhat better to read that others are disheartened by the lack of respect > for the effort and time that it can take to become a certified, experienced > histotech. The pay doesn't really bother me as much as the attitude that I > encounter on a daily basis that "anyone" can do histology and that it is to > quote "no big deal" to get certified. I have to concur that in my experience, > histology managers do not seem to value or even recognize the skills and time > it takes to perfect this trade.I know for me, getting an HTL was quite a > burden at times. I had extra studies for sure. And, back in that day, the ASCP > slide practical was no picnic. At my own hospital, this attitude is epitomized > by the fact that routinely non-certifed, non-histology people are both hired > and promoted. I have never seen anyone with a histology background given any > sort of professional respect like that given freely to the MT personnel that > work there. They do not even recgonize an HTL as a certification. They only > give you a small increase for being certified at all (HT). At particularly bad > moments, this had made me want to leave the field as well. So, I can relate > very easily to everyone else's sentiments. This hospital has been an > especially bad example of the "warm body" syndrome. It is almost crippling in > terms of the quality of the work and TAT. The inferior service and poor > quality produced only serves to reinforce the negative concept- it is indeed a > vicious cycle! > At least (all of us) are not alone! > Joelle > _________________________________________________________________ > Keep your kids safer online with Windows Live Family Safety. > http://www.windowslive.com/family_safety/overview.html?ocid=TXT_TAGLM_WL_Refre > sh_family_safety_052008_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Larry A. Woody Seattle, Wa. From CIngles <@t> uwhealth.org Tue May 20 17:11:09 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Tue May 20 17:12:34 2008 Subject: [Histonet] NSH bulletin (In Action) References: <052020081852.19529.48331DF400087B3200004C4922218675169B0A02D2089B9A019C04040A0DBFCE9C07089B0E03030C@att.net> <4832EA8F.6EA2.00FD.0@vetmed.ufl.edu> Message-ID: <08A0A863637F1349BBFD83A96B27A50A120133@uwhis-xchng3.uwhis.hosp.wisc.edu> I thought histotechs in Florida had to have a Florida liscense already or am I just confused? (it's been known to happen...) Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of MaryAnn Dixon Sent: Tue 5/20/2008 2:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NSH bulletin (In Action) Hi Histonetters, Has anyone read the In Action NSH bulletin lately? Just another example of the lack of recognition for the field of Histology. I am new to the field of Histology. What has happened to those employed in the NY labs? Are other states following by example? Should I get out now before I am unemployed? MaryAnn Dixon Biological Scientist Anatomic Pathology University of Florida Medical Center 352-392-2235 ext 4517 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcox90 <@t> yahoo.com Tue May 20 18:05:40 2008 From: jcox90 <@t> yahoo.com (Jill Cox) Date: Tue May 20 18:05:44 2008 Subject: [Histonet] Pathology Report Template Message-ID: <837412.4281.qm@web56805.mail.re3.yahoo.com> Hi Netters!! I am setting up a new lab and looking for a Dermatology Pathology report template. Do you know where we can purchase one or find one on Internet? I have been looking and not been very successful. Thanks in advance!! Jill Cox HT (ASCP) From AnthonyH <@t> chw.edu.au Tue May 20 23:41:13 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue May 20 23:41:29 2008 Subject: [Histonet] Microwave deparaffinization. In-Reply-To: <8546b4020805191236y3c9cad39k8f6f1bd3a2f3302b@mail.gmail.com> Message-ID: Sebastian, The following article uses a heated detergent solution to dewax and rehydrate sections. I have not as yet used it myself but I plan to try it at some time. Lars Falkeholm, Crawford A. Grant, Anders Magnusson, and Eva M?ller (2001) "Xylene-Free Method for Histological Preparation: A Multicentre Evaluation" LABORATORY INVESTIGATION Vol. 81, No. 9, p. 1213-1221. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebasti?n Vecchio Sent: Tuesday, 20 May 2008 5:36 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave deparaffinization. I have been trying to deparafinize section in the lab, but I cannot get the right time and power for the MW oven, does any one has a protocol for this . This method eliminates the uses of xylol in the processing of staining sections. Thanks. Sebastian, Student of Histotechnology, EUTM, FMED, UDELAR. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From annigyg <@t> gmail.com Wed May 21 01:15:33 2008 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Wed May 21 01:15:39 2008 Subject: [Histonet] Microwave deparaffinization. In-Reply-To: References: <8546b4020805191236y3c9cad39k8f6f1bd3a2f3302b@mail.gmail.com> Message-ID: as per the DAKO PT link procedure we have tried dewaxing slides for IHC using our standard buffers (with tween) and heating them up to the recommended temps - works like a charm ER and PR looking good i could not believe when reading the 'blurb' that the solution used to 'boil' out the wax would not leave a greasy residue on the slides as they are removed and cooler air congeals the waxy/buffer on the slides but i was wrong - try it out Annie 2008/5/21 Tony Henwood : > Sebastian, > The following article uses a heated detergent solution to dewax and > rehydrate sections. I have not as yet used it myself but I plan to try it at > some time. > > Lars Falkeholm, Crawford A. Grant, Anders Magnusson, and Eva M?ller (2001) > "Xylene-Free Method for Histological Preparation: A Multicentre Evaluation" > LABORATORY INVESTIGATION Vol. 81, No. 9, p. 1213-1221. > > > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) > Laboratory Manager & Senior Scientist > The Children's Hospital at Westmead, > Locked Bag 4001, Westmead, 2145, AUSTRALIA. > Tel: 612 9845 3306 > Fax: 612 9845 3318 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebasti?n Vecchio > Sent: Tuesday, 20 May 2008 5:36 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Microwave deparaffinization. > > > I have been trying to deparafinize section in the lab, but I cannot get > the right time and power for the MW oven, does any one has a protocol for > this . This method eliminates the uses of xylol in the processing of > staining sections. Thanks. Sebastian, Student of Histotechnology, EUTM, > FMED, UDELAR. _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************* > This email and any files transmitted with it are confidential and intended > solely for the use of the individual or entity to whom they are addressed. > If you are not the intended recipient, please delete it and notify the > sender. > > Views expressed in this message and any attachments are those of the > individual sender, and are not necessarily the views of The Children's > Hospital at Westmead > > This note also confirms that this email message has been > virus scanned and although no computer viruses were detected, The Childrens > Hospital at Westmead accepts no liability for any consequential damage > resulting from email containing computer viruses. > ********************************************************************** > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From lpwenk <@t> sbcglobal.net Wed May 21 04:52:31 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed May 21 04:52:36 2008 Subject: [Histonet] Formaldehyde + Acetic Acid In-Reply-To: <383417.87568.qm@web65703.mail.ac4.yahoo.com> Message-ID: <000601c8bb28$6357bf00$3fe42d4b@HPPav2> Just a thought - Could they have used neutral buffered formalin instead of formaldehyde? The buffering salts could precipitate out in alcohol. Lee & Peggy Wenk -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, May 20, 2008 8:58 AM To: Jennifer Johnson; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Formaldehyde + Acetic Acid Not even that: FAA (formaldehyde + alcohol + acetic acid) + formalin do NOT produce any milky solution. Tell you PT to check what s/he was mixing, that had to be something else in that mixture. Ren? J. Jennifer Johnson wrote: _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmjohnson34 <@t> hotmail.com Wed May 21 06:38:48 2008 From: jmjohnson34 <@t> hotmail.com (Jennifer Johnson) Date: Wed May 21 06:38:55 2008 Subject: [Histonet] Formaldehyde + Acetic Acid In-Reply-To: <000601c8bb28$6357bf00$3fe42d4b@HPPav2> References: <383417.87568.qm@web65703.mail.ac4.yahoo.com> <000601c8bb28$6357bf00$3fe42d4b@HPPav2> Message-ID: Yes, It was NBF. What started this whole mess was that my pathologist keeps a small holding tank of "somewhat clean?" NB formalin that he pours out of the biopsy containers when he grosses (He lived through the great depression). Then, he stores the basket from the processor in there while he grosses and at the end of the day, transfers it into the processor and starts it. One day, I had put a large breast specimen in FAA and he poured the FAA into the "holding tank" not knowing that it wasn't pure 10% NB formalin. (Yes, he is hard of smelling) It ruined all of the bloody specimens such as placenta (I assume because they are never fixed when they come down and the acetic acid lysed the red blood cells in it turning it to mush?) but everything else seemed to fair well. Now he is trying to figure out what the chemical reaction was that caused the problem. > From: lpwenk@sbcglobal.net> To: rjbuesa@yahoo.com; jmjohnson34@hotmail.com; histonet@lists.utsouthwestern.edu> Subject: RE: [Histonet] Formaldehyde + Acetic Acid> Date: Wed, 21 May 2008 05:52:31 -0400> > Just a thought - Could they have used neutral buffered formalin instead of> formaldehyde? The buffering salts could precipitate out in alcohol. > > > Lee & Peggy Wenk> -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa> Sent: Tuesday, May 20, 2008 8:58 AM> To: Jennifer Johnson; histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Formaldehyde + Acetic Acid> > Not even that: FAA (formaldehyde + alcohol + acetic acid) + formalin do NOT> produce any milky solution. Tell you PT to check what s/he was mixing, that> had to be something else in that mixture.> Ren? J.> > Jennifer Johnson wrote:> > > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> _________________________________________________________________ Give to a good cause with every e-mail. Join the i?m Initiative from Microsoft. http://im.live.com/Messenger/IM/Join/Default.aspx?souce=EML_WL_ GoodCause From rjbuesa <@t> yahoo.com Wed May 21 07:50:17 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 21 07:50:22 2008 Subject: [Histonet] Sample antibodies In-Reply-To: <052020081852.19529.48331DF400087B3200004C4922218675169B0A02D2089B9A019C04040A0DBFCE9C07089B0E03030C@att.net> Message-ID: <125442.28942.qm@web65704.mail.ac4.yahoo.com> My DAKO representative regularly used to give me samples of antibodies there were starting to market, or from some I wanted to test before using them regularly. Perhaps you could contact DAKO Ren? J. cmmathis1@bellsouth.net wrote: Are there any companies that have sample antibodies or any kind of trial size? Cathy Mathis _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed May 21 07:58:23 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 21 07:58:32 2008 Subject: [Histonet] NSH bulletin (In Action) In-Reply-To: <4832EA8F.6EA2.00FD.0@vetmed.ufl.edu> Message-ID: <36038.98450.qm@web65712.mail.ac4.yahoo.com> MaryAnn: In countries with structured laboratory professionals licenses (like Canada, South Africa, the UK, Austria, Spain) all laboratory professionals have a common license level divided into specialties, "histology" being one of them. In those countries (in contrast to ours) all laboratory professionals earn the same basic salary regardless of the specialty, and that is exactly was is missing here and why histotechs are at the salary bottom scale in the medical lab. Perhaps we should try to get a similar situation, which is similar to what NY has done now. There should be just one license for all laboratory professionals, divided by specialties. I think this will eliminate the salary disparities now existing between MT and HT Done in an orderly way and with the required "grace periods" I think this is the correct approach. Ren? J. MaryAnn Dixon wrote: Hi Histonetters, Has anyone read the In Action NSH bulletin lately? Just another example of the lack of recognition for the field of Histology. I am new to the field of Histology. What has happened to those employed in the NY labs? Are other states following by example? Should I get out now before I am unemployed? MaryAnn Dixon Biological Scientist Anatomic Pathology University of Florida Medical Center 352-392-2235 ext 4517 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed May 21 08:09:19 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 21 08:09:24 2008 Subject: [Histonet] histotech pay/respect In-Reply-To: <1F937FB30BDB7C4A9F39F83FEA8D379F9F54DB@bruexchange1.digestivespecialists.com> Message-ID: <108885.49172.qm@web65709.mail.ac4.yahoo.com> Having a good experience with a pathologist (or even with several) is a sign of "good luck", like ending with a good spouse. We histotech should not be subjected to the "good (or bad) whims of pathologists", it should be a RIGHT to earn decent salaries and be treated with respect. I am sure that during slavery times, there were bad and "not so bad" plantation owners! Ren? J. "Blazek, Linda" wrote: Patti and all, Horary Patti. I was just sitting here getting ready to post my email when you posted yours. I was beginning to think I was the only one here that has had a very positive experience as a histotech. I've been watching this topic for a long time now and wonder where I've been. I've been a histotech since the early 70's. The pathologist I worked for in the early 80's wanted to know why I never took my registry when I finished school. I guess a baby got in the way and no one really required it. He pushed and encouraged me to take my registry. He was so supportive and helpful to someone that waited 9 years to sit for the exam that it was amazing. I did receive a healthy raise when I passed. I have never in all these years worked for a pathologist that wasn't respectful and included me as a part of a team. I have had a bit of "red-headed stepchild" reactions from MTs but that never bothered me since none of them had a clue as to what a histotech did. When they removed the histology rotation from the MT training in the early 70's they didn't have any exposure to our world. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Tuesday, May 20, 2008 2:45 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect Joelle, Best pathologist quote that I've ever heard myself: "Let's face it, a trained monkey can cut blocks." This was referring to trying to staff his personal research lab and was said with all seriousness. Needless to say, this pathologist and I didn't get along well at all. Unfortunately, too many share these sentiments and don't have a clue that a histotech tends to do a bit more than "cut blocks". Sort of makes you wonder why any of us bothers with getting certified or with obtaining necessary CEU's to stay that way. Hang in There, Glen Dawson BS, HT & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of joelle weaver Sent: Tuesday, May 20, 2008 12:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] histotech pay/respect In response to several postings regarding pay and respect- I have been feeling somewhat better to read that others are disheartened by the lack of respect for the effort and time that it can take to become a certified, experienced histotech. The pay doesn't really bother me as much as the attitude that I encounter on a daily basis that "anyone" can do histology and that it is to quote "no big deal" to get certified. I have to concur that in my experience, histology managers do not seem to value or even recognize the skills and time it takes to perfect this trade.I know for me, getting an HTL was quite a burden at times. I had extra studies for sure. And, back in that day, the ASCP slide practical was no picnic. At my own hospital, this attitude is epitomized by the fact that routinely non-certifed, non-histology people are both hired and promoted. I have never seen anyone with a histology background given any sort of professional respect like that given freely to the MT personnel that work there. They do not even recgonize an HTL as a certification. They only give you a small increase for being certified at all (HT). At particularly bad moments, this had made me want to leave the field as well. So, I can relate very easily to everyone else's sentiments. This hospital has been an especially bad example of the "warm body" syndrome. It is almost crippling in terms of the quality of the work and TAT. The inferior service and poor quality produced only serves to reinforce the negative concept- it is indeed a vicious cycle! At least (all of us) are not alone! Joelle _________________________________________________________________ Keep your kids safer online with Windows Live Family Safety. http://www.windowslive.com/family_safety/overview.html?ocid=TXT_TAGLM_WL _Refresh_family_safety_052008___________________________________________ ____ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed May 21 08:17:21 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 21 08:17:28 2008 Subject: [Histonet] NSH bulletin (In Action) In-Reply-To: <08A0A863637F1349BBFD83A96B27A50A120133@uwhis-xchng3.uwhis.hosp.wisc.edu> Message-ID: <980210.74501.qm@web65714.mail.ac4.yahoo.com> Florida requires licenses to practice histology but they are apart from any other area of the medical lab that has licenses for each area (chemistry, hematology, blood bank, etc). Ren? J. Ingles Claire wrote: I thought histotechs in Florida had to have a Florida liscense already or am I just confused? (it's been known to happen...) Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of MaryAnn Dixon Sent: Tue 5/20/2008 2:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NSH bulletin (In Action) Hi Histonetters, Has anyone read the In Action NSH bulletin lately? Just another example of the lack of recognition for the field of Histology. I am new to the field of Histology. What has happened to those employed in the NY labs? Are other states following by example? Should I get out now before I am unemployed? MaryAnn Dixon Biological Scientist Anatomic Pathology University of Florida Medical Center 352-392-2235 ext 4517 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Wed May 21 08:25:36 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed May 21 08:20:27 2008 Subject: [Histonet] histotech pay/respect In-Reply-To: <108885.49172.qm@web65709.mail.ac4.yahoo.com> References: <1F937FB30BDB7C4A9F39F83FEA8D379F9F54DB@bruexchange1.digestivespecialists.com> <108885.49172.qm@web65709.mail.ac4.yahoo.com> Message-ID: <1F937FB30BDB7C4A9F39F83FEA8D379F9F54E1@bruexchange1.digestivespecialists.com> Sorry Ren? but I don't think having worked with 12 different pathologists and being treated with respect by ALL of them is a matter of good luck. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com ________________________________ From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Wednesday, May 21, 2008 9:09 AM To: Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect Having a good experience with a pathologist (or even with several) is a sign of "good luck", like ending with a good spouse. We histotech should not be subjected to the "good (or bad) whims of pathologists", it should be a RIGHT to earn decent salaries and be treated with respect. I am sure that during slavery times, there were bad and "not so bad" plantation owners! Ren? J. "Blazek, Linda" wrote: Patti and all, Horary Patti. I was just sitting here getting ready to post my email when you posted yours. I was beginning to think I was the only one here that has had a very positive experience as a histotech. I've been watching this topic for a long time now and wonder where I've been. I've been a histotech since the early 70's. The pathologist I worked for in the early 80's wanted to know why I never took my registry when I finished school. I guess a baby got in the way and no one really required it. He pushed and encouraged me to take my registry. He was so supportive and helpful to someone that waited 9 years to sit for the exam that it was amazing. I did receive a healthy raise when I passed. I have never in all these years worked for a pathologist that wasn't respectful and included me as a part of a team. I have had a bit of "red-headed stepchild" reactions from MTs but that never bothered me since none of them had a clue as to what a histotech did. When they removed the histology rotation from the MT training in the early 70's they didn't have any exposure to our world. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Tuesday, May 20, 2008 2:45 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect Joelle, Best pathologist quote that I've ever heard myself: "Let's face it, a trained monkey can cut blocks." This was referring to trying to staff his personal research lab and was said with all seriousness. Needless to say, this pathologist and I didn't get along well at all. Unfortunately, too many share these sentiments and don't have a clue that a histotech tends to do a bit more than "cut blocks". Sort of makes you wonder why any of us bothers with getting certified or with obtaining necessary CEU's to stay that way. Hang in There, Glen Dawson BS, HT & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of joelle weaver Sent: Tuesday, May 20, 2008 12:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] histotech pay/respect In response to several postings regarding pay and respect- I have been feeling somewhat better to read that others are disheartened by the lack of respect for the effort and time that it can take to become a certified, experienced histotech. The pay doesn't really bother me as much as the attitude that I encounter on a daily basis that "anyone" can do histology and that it is to quote "no big deal" to get certified. I have to concur that in my experience, histology managers do not seem to value or even recognize the skills and time it takes to perfect this trade.I know for me, getting an HTL was quite a burden at times. I had extra studies for sure. And, back in that day, the ASCP slide practical was no picnic. At my own hospital, this attitude is epitomized by the fact that routinely non-certifed, non-histology people are both hired and promoted. I have never seen anyone with a histology background given any sort of professional respect like that given freely to the MT personnel that work there. They do not even recgonize an HTL as a certification. They only give you a small increase for being certified at all (HT). At particularly bad moments, this had made me want to leave the field as well. So, I can relate very easily to everyone else's sentiments. This hospital has been an especially bad example of the "warm body" syndrome. It is almost crippling in terms of the quality of the work and TAT. The inferior service and poor quality produced only serves to reinforce the negative concept- it is indeed a vicious cycle! At least (all of us) are not alone! Joelle _________________________________________________________________ Keep your kids safer online with Windows Live Family Safety. http://www.windowslive.com/family_safety/overview.html?ocid=TXT_TAGLM_WL _Refresh_family_safety_052008___________________________________________ ____ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed May 21 08:29:18 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 21 08:29:22 2008 Subject: [Histonet] Formaldehyde + Acetic Acid In-Reply-To: Message-ID: <901731.78152.qm@web65708.mail.ac4.yahoo.com> Peggy showed why she had been awarded "Histotech of the Year" with her very clever and insightful answer. The salts dissolved in the NBF will precipitate with the alcohol. That is why the tissue processors have to be cleaned with hot hater (to eliminate the salts by dissolving them), or why some people (like me) used to use 10% formalin NOT buffered in the first 2 stations of the VIP (to avoid the salt precipitates). Ren? J. Jennifer Johnson wrote: .hmmessage P { margin:0px; padding:0px } body.hmmessage { FONT-SIZE: 10pt; FONT-FAMILY:Tahoma } > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > --------------------------------- Give to a good cause with every e-mail. Join the i?m Initiative from Microsoft. From galalmkh <@t> yahoo.com Wed May 21 08:55:04 2008 From: galalmkh <@t> yahoo.com (manal galal) Date: Wed May 21 08:55:12 2008 Subject: [Histonet] modified gomori stain Message-ID: <866479.14253.qm@web50212.mail.re2.yahoo.com> Hi I need help with modified gomori stain for muscle biopsies. I cant seem to get the muscle fiber to turn green. everytime I put the acetic acid for differentiation, the fibers loose all the color. can anyone help me. Manal Galal cairo From Gguerzon <@t> lifebridgehealth.org Wed May 21 08:55:18 2008 From: Gguerzon <@t> lifebridgehealth.org (Godfrey Guerzon) Date: Wed May 21 08:55:35 2008 Subject: [Histonet] histotech pay/respect In-Reply-To: <1F937FB30BDB7C4A9F39F83FEA8D379F9F54E1@bruexchange1.digestivespecialists.com> References: <1F937FB30BDB7C4A9F39F83FEA8D379F9F54DB@bruexchange1.digestivespecialists.com> <108885.49172.qm@web65709.mail.ac4.yahoo.com> <1F937FB30BDB7C4A9F39F83FEA8D379F9F54E1@bruexchange1.digestivespecialists.com> Message-ID: <4833F186.704A.0068.0@lifebridgehealth.org> I agree with you Linda. I have worked with a lot of Pathologists and I was treated with respect by all of them. Maybe not at the beginning, but later on all treated me with respect. I believe like trust - respect is earned. To be respected you must be respectful. The world and its citizens responds to what you dish out accordingly. Godfrey >>> "Blazek, Linda" 5/21/2008 9:25 AM >>> Sorry Ren? but I don't think having worked with 12 different pathologists and being treated with respect by ALL of them is a matter of good luck. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com ________________________________ From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Wednesday, May 21, 2008 9:09 AM To: Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect Having a good experience with a pathologist (or even with several) is a sign of "good luck", like ending with a good spouse. We histotech should not be subjected to the "good (or bad) whims of pathologists", it should be a RIGHT to earn decent salaries and be treated with respect. I am sure that during slavery times, there were bad and "not so bad" plantation owners! Ren? J. "Blazek, Linda" wrote: Patti and all, Horary Patti. I was just sitting here getting ready to post my email when you posted yours. I was beginning to think I was the only one here that has had a very positive experience as a histotech. I've been watching this topic for a long time now and wonder where I've been. I've been a histotech since the early 70's. The pathologist I worked for in the early 80's wanted to know why I never took my registry when I finished school. I guess a baby got in the way and no one really required it. He pushed and encouraged me to take my registry. He was so supportive and helpful to someone that waited 9 years to sit for the exam that it was amazing. I did receive a healthy raise when I passed. I have never in all these years worked for a pathologist that wasn't respectful and included me as a part of a team. I have had a bit of "red-headed stepchild" reactions from MTs but that never bothered me since none of them had a clue as to what a histotech did. When they removed the histology rotation from the MT training in the early 70's they didn't have any exposure to our world. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Tuesday, May 20, 2008 2:45 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect Joelle, Best pathologist quote that I've ever heard myself: "Let's face it, a trained monkey can cut blocks." This was referring to trying to staff his personal research lab and was said with all seriousness. Needless to say, this pathologist and I didn't get along well at all. Unfortunately, too many share these sentiments and don't have a clue that a histotech tends to do a bit more than "cut blocks". Sort of makes you wonder why any of us bothers with getting certified or with obtaining necessary CEU's to stay that way. Hang in There, Glen Dawson BS, HT & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of joelle weaver Sent: Tuesday, May 20, 2008 12:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] histotech pay/respect In response to several postings regarding pay and respect- I have been feeling somewhat better to read that others are disheartened by the lack of respect for the effort and time that it can take to become a certified, experienced histotech. The pay doesn't really bother me as much as the attitude that I encounter on a daily basis that "anyone" can do histology and that it is to quote "no big deal" to get certified. I have to concur that in my experience, histology managers do not seem to value or even recognize the skills and time it takes to perfect this trade.I know for me, getting an HTL was quite a burden at times. I had extra studies for sure. And, back in that day, the ASCP slide practical was no picnic. At my own hospital, this attitude is epitomized by the fact that routinely non-certifed, non-histology people are both hired and promoted. I have never seen anyone with a histology background given any sort of professional respect like that given freely to the MT personnel that work there. They do not even recgonize an HTL as a certification. They only give you a small increase for being certified at all (HT). At particularly bad moments, this had made me want to leave the field as well. So, I can relate very easily to everyone else's sentiments. This hospital has been an especially bad example of the "warm body" syndrome. It is almost crippling in terms of the quality of the work and TAT. The inferior service and poor quality produced only serves to reinforce the negative concept- it is indeed a vicious cycle! At least (all of us) are not alone! Joelle _________________________________________________________________ Keep your kids safer online with Windows Live Family Safety. http://www.windowslive.com/family_safety/overview.html?ocid=TXT_TAGLM_WL _Refresh_family_safety_052008___________________________________________ ____ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------------------------------- THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE ADDRESSEE LISTED ABOVE. This record has been disclosed in accordance with Subtitle 3 of Title 4 of the Health-General Article of the Annotated Code of Maryland. Further disclosure of medical information contained herein is prohibited. If you are neither the intended recipient nor the individual responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure of patient information is strictly prohibited. If you have received this email in error, immediately notify us by telephone or return email. ----------------------------------------------------------------- From jqb7 <@t> cdc.gov Wed May 21 09:03:15 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed May 21 09:05:04 2008 Subject: [Histonet] histotech pay/respect In-Reply-To: <1F937FB30BDB7C4A9F39F83FEA8D379F9F54E1@bruexchange1.digestivespecialists.com> References: <1F937FB30BDB7C4A9F39F83FEA8D379F9F54DB@bruexchange1.digestivespecialists.com> <108885.49172.qm@web65709.mail.ac4.yahoo.com> <1F937FB30BDB7C4A9F39F83FEA8D379F9F54E1@bruexchange1.digestivespecialists.com> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A747A394@LTA3VS011.ees.hhs.gov> I have to say that the majority of pathologists I have worked with over the years have treated me with respect but not all. And the ones that did not treat me respectfully did not treat any of the laboratorians with respect. So I can't say respect is always earned. There are often a few bad eggs in the mix. I can remember as a student (back in the day) one of the pathologists came into the lab with a tray of H&E's and shouted, "Who cut this sh**?!" Then he threw the tray on the floor and broke all of the slides. Now I am sure that type of behavior would never be allowed today. But honestly, over the many years I have been in histology I can say only 2 have been truly disrespectful. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Wednesday, May 21, 2008 9:26 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect Sorry Ren? but I don't think having worked with 12 different pathologists and being treated with respect by ALL of them is a matter of good luck. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com ________________________________ From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Wednesday, May 21, 2008 9:09 AM To: Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect Having a good experience with a pathologist (or even with several) is a sign of "good luck", like ending with a good spouse. We histotech should not be subjected to the "good (or bad) whims of pathologists", it should be a RIGHT to earn decent salaries and be treated with respect. I am sure that during slavery times, there were bad and "not so bad" plantation owners! Ren? J. "Blazek, Linda" wrote: Patti and all, Horary Patti. I was just sitting here getting ready to post my email when you posted yours. I was beginning to think I was the only one here that has had a very positive experience as a histotech. I've been watching this topic for a long time now and wonder where I've been. I've been a histotech since the early 70's. The pathologist I worked for in the early 80's wanted to know why I never took my registry when I finished school. I guess a baby got in the way and no one really required it. He pushed and encouraged me to take my registry. He was so supportive and helpful to someone that waited 9 years to sit for the exam that it was amazing. I did receive a healthy raise when I passed. I have never in all these years worked for a pathologist that wasn't respectful and included me as a part of a team. I have had a bit of "red-headed stepchild" reactions from MTs but that never bothered me since none of them had a clue as to what a histotech did. When they removed the histology rotation from the MT training in the early 70's they didn't have any exposure to our world. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Tuesday, May 20, 2008 2:45 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect Joelle, Best pathologist quote that I've ever heard myself: "Let's face it, a trained monkey can cut blocks." This was referring to trying to staff his personal research lab and was said with all seriousness. Needless to say, this pathologist and I didn't get along well at all. Unfortunately, too many share these sentiments and don't have a clue that a histotech tends to do a bit more than "cut blocks". Sort of makes you wonder why any of us bothers with getting certified or with obtaining necessary CEU's to stay that way. Hang in There, Glen Dawson BS, HT & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of joelle weaver Sent: Tuesday, May 20, 2008 12:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] histotech pay/respect In response to several postings regarding pay and respect- I have been feeling somewhat better to read that others are disheartened by the lack of respect for the effort and time that it can take to become a certified, experienced histotech. The pay doesn't really bother me as much as the attitude that I encounter on a daily basis that "anyone" can do histology and that it is to quote "no big deal" to get certified. I have to concur that in my experience, histology managers do not seem to value or even recognize the skills and time it takes to perfect this trade.I know for me, getting an HTL was quite a burden at times. I had extra studies for sure. And, back in that day, the ASCP slide practical was no picnic. At my own hospital, this attitude is epitomized by the fact that routinely non-certifed, non-histology people are both hired and promoted. I have never seen anyone with a histology background given any sort of professional respect like that given freely to the MT personnel that work there. They do not even recgonize an HTL as a certification. They only give you a small increase for being certified at all (HT). At particularly bad moments, this had made me want to leave the field as well. So, I can relate very easily to everyone else's sentiments. This hospital has been an especially bad example of the "warm body" syndrome. It is almost crippling in terms of the quality of the work and TAT. The inferior service and poor quality produced only serves to reinforce the negative concept- it is indeed a vicious cycle! At least (all of us) are not alone! Joelle _________________________________________________________________ Keep your kids safer online with Windows Live Family Safety. http://www.windowslive.com/family_safety/overview.html?ocid=TXT_TAGLM_WL _Refresh_family_safety_052008___________________________________________ ____ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Wed May 21 09:17:07 2008 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Wed May 21 09:17:21 2008 Subject: [Histonet] histotech pay/respect Message-ID: <5C0BED61F529364E86309CADEA63FEF20163F3FF@TRFT-EX01.xRothGen.nhs.uk> Gee, the number of times I would liked to have done that:-) Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 21 May 2008 15:03 To: Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect I have to say that the majority of pathologists I have worked with over the years have treated me with respect but not all. And the ones that did not treat me respectfully did not treat any of the laboratorians with respect. So I can't say respect is always earned. There are often a few bad eggs in the mix. I can remember as a student (back in the day) one of the pathologists came into the lab with a tray of H&E's and shouted, "Who cut this sh**?!" Then he threw the tray on the floor and broke all of the slides. Now I am sure that type of behavior would never be allowed today. But honestly, over the many years I have been in histology I can say only 2 have been truly disrespectful. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Wednesday, May 21, 2008 9:26 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect Sorry Ren? but I don't think having worked with 12 different pathologists and being treated with respect by ALL of them is a matter of good luck. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com ________________________________ From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Wednesday, May 21, 2008 9:09 AM To: Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect Having a good experience with a pathologist (or even with several) is a sign of "good luck", like ending with a good spouse. We histotech should not be subjected to the "good (or bad) whims of pathologists", it should be a RIGHT to earn decent salaries and be treated with respect. I am sure that during slavery times, there were bad and "not so bad" plantation owners! Ren? J. "Blazek, Linda" wrote: Patti and all, Horary Patti. I was just sitting here getting ready to post my email when you posted yours. I was beginning to think I was the only one here that has had a very positive experience as a histotech. I've been watching this topic for a long time now and wonder where I've been. I've been a histotech since the early 70's. The pathologist I worked for in the early 80's wanted to know why I never took my registry when I finished school. I guess a baby got in the way and no one really required it. He pushed and encouraged me to take my registry. He was so supportive and helpful to someone that waited 9 years to sit for the exam that it was amazing. I did receive a healthy raise when I passed. I have never in all these years worked for a pathologist that wasn't respectful and included me as a part of a team. I have had a bit of "red-headed stepchild" reactions from MTs but that never bothered me since none of them had a clue as to what a histotech did. When they removed the histology rotation from the MT training in the early 70's they didn't have any exposure to our world. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Tuesday, May 20, 2008 2:45 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect Joelle, Best pathologist quote that I've ever heard myself: "Let's face it, a trained monkey can cut blocks." This was referring to trying to staff his personal research lab and was said with all seriousness. Needless to say, this pathologist and I didn't get along well at all. Unfortunately, too many share these sentiments and don't have a clue that a histotech tends to do a bit more than "cut blocks". Sort of makes you wonder why any of us bothers with getting certified or with obtaining necessary CEU's to stay that way. Hang in There, Glen Dawson BS, HT & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of joelle weaver Sent: Tuesday, May 20, 2008 12:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] histotech pay/respect In response to several postings regarding pay and respect- I have been feeling somewhat better to read that others are disheartened by the lack of respect for the effort and time that it can take to become a certified, experienced histotech. The pay doesn't really bother me as much as the attitude that I encounter on a daily basis that "anyone" can do histology and that it is to quote "no big deal" to get certified. I have to concur that in my experience, histology managers do not seem to value or even recognize the skills and time it takes to perfect this trade.I know for me, getting an HTL was quite a burden at times. I had extra studies for sure. And, back in that day, the ASCP slide practical was no picnic. At my own hospital, this attitude is epitomized by the fact that routinely non-certifed, non-histology people are both hired and promoted. I have never seen anyone with a histology background given any sort of professional respect like that given freely to the MT personnel that work there. They do not even recgonize an HTL as a certification. They only give you a small increase for being certified at all (HT). At particularly bad moments, this had made me want to leave the field as well. So, I can relate very easily to everyone else's sentiments. This hospital has been an especially bad example of the "warm body" syndrome. It is almost crippling in terms of the quality of the work and TAT. The inferior service and poor quality produced only serves to reinforce the negative concept- it is indeed a vicious cycle! At least (all of us) are not alone! Joelle _________________________________________________________________ Keep your kids safer online with Windows Live Family Safety. http://www.windowslive.com/family_safety/overview.html?ocid=TXT_TAGLM_WL _Refresh_family_safety_052008___________________________________________ ____ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jennifer.l.hofecker <@t> Vanderbilt.Edu Wed May 21 09:27:17 2008 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Wed May 21 09:27:24 2008 Subject: [Histonet] modified Gomori stain In-Reply-To: <866479.14253.qm@web50212.mail.re2.yahoo.com> References: <866479.14253.qm@web50212.mail.re2.yahoo.com> Message-ID: <898D946569A27444B65667A49C0740520175B4BF@mailbe06.mc.vanderbilt.edu> Hi there. I have two suggestions for you. The first would be to check the pH of your Gomori trichrome solution. Strange things happen to muscles when the pH strays from 3.4! The second suggestion would be to just skip the acid differentiation, provided the sections are green when they come out of the stain. I did that once, accidentally, and the pathologist was able to interpret the slides. If the sections are predominately red after the staining step, check the pH. Please let me know if you need any further information. Good Luck! Jennifer L. Hofecker HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph 615.343.0083 fax 615.343.7089 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of manal galal Sent: Wednesday, May 21, 2008 8:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] modified gomori stain Hi I need help with modified gomori stain for muscle biopsies. I cant seem to get the muscle fiber to turn green. everytime I put the acetic acid for differentiation, the fibers loose all the color. can anyone help me. Manal Galal cairo _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed May 21 09:28:41 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 21 09:28:44 2008 Subject: [Histonet] modified gomori stain In-Reply-To: <866479.14253.qm@web50212.mail.re2.yahoo.com> Message-ID: <214249.32718.qm@web65711.mail.ac4.yahoo.com> Prepare a fresh green staining solution and try again! Ren? J. manal galal wrote: Hi I need help with modified gomori stain for muscle biopsies. I cant seem to get the muscle fiber to turn green. everytime I put the acetic acid for differentiation, the fibers loose all the color. can anyone help me. Manal Galal cairo _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jennifer.l.hofecker <@t> Vanderbilt.Edu Wed May 21 09:37:21 2008 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Wed May 21 09:37:30 2008 Subject: [Histonet] histotech pay/respect Message-ID: <898D946569A27444B65667A49C0740520175B4C0@mailbe06.mc.vanderbilt.edu> I would also like to "chime in" and say that throughout my career, I have been very blessed to work with wonderful pathologists. The majority of them supported and encouraged my professional development. Unfortunately much of the time, their hands are tied when it comes to salaries. I have learned that although money is nice, it is quite rewarding to work with people who respect me and value my contributions to patient care. I agree, there have been a few "bad apples" who did not treat me with respect. That's when it's time to polish up the resume. Heading into the bunker now... Jennifer L. Hofecker HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph 615.343.0083 fax 615.343.7089 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Wednesday, May 21, 2008 9:17 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect Gee, the number of times I would liked to have done that:-) Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 21 May 2008 15:03 To: Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect I have to say that the majority of pathologists I have worked with over the years have treated me with respect but not all. And the ones that did not treat me respectfully did not treat any of the laboratorians with respect. So I can't say respect is always earned. There are often a few bad eggs in the mix. I can remember as a student (back in the day) one of the pathologists came into the lab with a tray of H&E's and shouted, "Who cut this sh**?!" Then he threw the tray on the floor and broke all of the slides. Now I am sure that type of behavior would never be allowed today. But honestly, over the many years I have been in histology I can say only 2 have been truly disrespectful. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Wednesday, May 21, 2008 9:26 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect Sorry Ren? but I don't think having worked with 12 different pathologists and being treated with respect by ALL of them is a matter of good luck. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com ________________________________ From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Wednesday, May 21, 2008 9:09 AM To: Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect Having a good experience with a pathologist (or even with several) is a sign of "good luck", like ending with a good spouse. We histotech should not be subjected to the "good (or bad) whims of pathologists", it should be a RIGHT to earn decent salaries and be treated with respect. I am sure that during slavery times, there were bad and "not so bad" plantation owners! Ren? J. "Blazek, Linda" wrote: Patti and all, Horary Patti. I was just sitting here getting ready to post my email when you posted yours. I was beginning to think I was the only one here that has had a very positive experience as a histotech. I've been watching this topic for a long time now and wonder where I've been. I've been a histotech since the early 70's. The pathologist I worked for in the early 80's wanted to know why I never took my registry when I finished school. I guess a baby got in the way and no one really required it. He pushed and encouraged me to take my registry. He was so supportive and helpful to someone that waited 9 years to sit for the exam that it was amazing. I did receive a healthy raise when I passed. I have never in all these years worked for a pathologist that wasn't respectful and included me as a part of a team. I have had a bit of "red-headed stepchild" reactions from MTs but that never bothered me since none of them had a clue as to what a histotech did. When they removed the histology rotation from the MT training in the early 70's they didn't have any exposure to our world. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Tuesday, May 20, 2008 2:45 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect Joelle, Best pathologist quote that I've ever heard myself: "Let's face it, a trained monkey can cut blocks." This was referring to trying to staff his personal research lab and was said with all seriousness. Needless to say, this pathologist and I didn't get along well at all. Unfortunately, too many share these sentiments and don't have a clue that a histotech tends to do a bit more than "cut blocks". Sort of makes you wonder why any of us bothers with getting certified or with obtaining necessary CEU's to stay that way. Hang in There, Glen Dawson BS, HT & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of joelle weaver Sent: Tuesday, May 20, 2008 12:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] histotech pay/respect In response to several postings regarding pay and respect- I have been feeling somewhat better to read that others are disheartened by the lack of respect for the effort and time that it can take to become a certified, experienced histotech. The pay doesn't really bother me as much as the attitude that I encounter on a daily basis that "anyone" can do histology and that it is to quote "no big deal" to get certified. I have to concur that in my experience, histology managers do not seem to value or even recognize the skills and time it takes to perfect this trade.I know for me, getting an HTL was quite a burden at times. I had extra studies for sure. And, back in that day, the ASCP slide practical was no picnic. At my own hospital, this attitude is epitomized by the fact that routinely non-certifed, non-histology people are both hired and promoted. I have never seen anyone with a histology background given any sort of professional respect like that given freely to the MT personnel that work there. They do not even recgonize an HTL as a certification. They only give you a small increase for being certified at all (HT). At particularly bad moments, this had made me want to leave the field as well. So, I can relate very easily to everyone else's sentiments. This hospital has been an especially bad example of the "warm body" syndrome. It is almost crippling in terms of the quality of the work and TAT. The inferior service and poor quality produced only serves to reinforce the negative concept- it is indeed a vicious cycle! At least (all of us) are not alone! Joelle _________________________________________________________________ Keep your kids safer online with Windows Live Family Safety. http://www.windowslive.com/family_safety/overview.html?ocid=TXT_TAGLM_WL _Refresh_family_safety_052008___________________________________________ ____ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alaskagirl1950 <@t> yahoo.com Wed May 21 09:40:39 2008 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Wed May 21 09:40:46 2008 Subject: [Histonet] Lab temperatures In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E630C@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <283278.71910.qm@web52501.mail.re2.yahoo.com> I have had problems like that, anywhere from 60F to 98F. Even in the Alabama Summer when you melt walking outside, we would freeze inside. I love it cool, from Alaska, but even I was going outside at lunch to warm up. Leaving in the evening, I would leave the windows up in the car until I was no longer Blue. Now when it was 98 degrees, my boss sent me home. And like you they say that the A/C is either on full force or off. But ours keeps breaking down. When it is impossible I just sit with the fan on me, blocks melting in the heat. Or sit bundled in sweat shirt and lab coats warming blocks just a little. I feel like one of the lab rats down stairs. Let's see how many extremes the human body can take. But all my Pathologists are wonderful to work with, so I stay. Homesteading spirit you know! Patricia From amh-histolab <@t> amh.org Wed May 21 09:40:49 2008 From: amh-histolab <@t> amh.org (AMH-HistoLab) Date: Wed May 21 09:41:25 2008 Subject: [Histonet] removing tissue from already H and E stained slides. Message-ID: I have a situation where a pathologist needs an Er and PR IHC done on a previous H and E stained slide. The slide has two sections on it and she asked if one section can be removed to another slide to perform ER on one slide and PR on the other. Does anyone know of a solution that will remove tissue safely from a slide after H and E stained and not interfere with IHC? Abington Memorial Hospital 1200 Old York Rd Abington, PA. 19001 ****************** CONFIDENTIALITY NOTICE ********************** This e-mail contains LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION intended only for the use of the recipient named above. If you are not the intended recipient, you are hereby notified that any dissemination or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please notify the transmitting hospital by telephone or e-mail and delete the original e-mail received in error. THIS INFORMATION HAS BEEN DISCLOSED TO YOU FROM RECORDS WHOSE CONFIDENTIALITY IS PROTECTED BY STATE AND FEDERAL LAW. ANY FURTHER DISCLOSURE, COPYING, DISTRIBUTION OR ACTION TAKEN IN RELIANCE ON THE CONTENTS OF THESE DOCUMENTS WITHOUT THE PRIOR WRITTEN CONSENT OF THE PERSON TO WHOM IT PERTAINS IS PROHIBITED. YOU ARE REQUIRED TO DESTROY THE INFORMATION AFTER THE STATED NEED HAS BEEN FULFILLED. From ian.montgomery <@t> bio.gla.ac.uk Wed May 21 09:43:34 2008 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Wed May 21 09:43:50 2008 Subject: FW: [Histonet] histotech pay/respect Message-ID: <7BF1D0D93E7F4F7483919CB83098E99A@IBLS.GLA.AC.UK> I've been following this discussion with interest as it's all so different here, we know our place. The master servant relationship is alive and well in the UK. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: 21 May 2008 15:17 To: Bartlett, Jeanine (CDC/CCID/NCZVED); Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect Gee, the number of times I would liked to have done that:-) Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 21 May 2008 15:03 To: Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect I have to say that the majority of pathologists I have worked with over the years have treated me with respect but not all. And the ones that did not treat me respectfully did not treat any of the laboratorians with respect. So I can't say respect is always earned. There are often a few bad eggs in the mix. I can remember as a student (back in the day) one of the pathologists came into the lab with a tray of H&E's and shouted, "Who cut this sh**?!" Then he threw the tray on the floor and broke all of the slides. Now I am sure that type of behavior would never be allowed today. But honestly, over the many years I have been in histology I can say only 2 have been truly disrespectful. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Wednesday, May 21, 2008 9:26 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect Sorry Ren? but I don't think having worked with 12 different pathologists and being treated with respect by ALL of them is a matter of good luck. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com ________________________________ From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Wednesday, May 21, 2008 9:09 AM To: Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect Having a good experience with a pathologist (or even with several) is a sign of "good luck", like ending with a good spouse. We histotech should not be subjected to the "good (or bad) whims of pathologists", it should be a RIGHT to earn decent salaries and be treated with respect. I am sure that during slavery times, there were bad and "not so bad" plantation owners! Ren? J. "Blazek, Linda" wrote: Patti and all, Horary Patti. I was just sitting here getting ready to post my email when you posted yours. I was beginning to think I was the only one here that has had a very positive experience as a histotech. I've been watching this topic for a long time now and wonder where I've been. I've been a histotech since the early 70's. The pathologist I worked for in the early 80's wanted to know why I never took my registry when I finished school. I guess a baby got in the way and no one really required it. He pushed and encouraged me to take my registry. He was so supportive and helpful to someone that waited 9 years to sit for the exam that it was amazing. I did receive a healthy raise when I passed. I have never in all these years worked for a pathologist that wasn't respectful and included me as a part of a team. I have had a bit of "red-headed stepchild" reactions from MTs but that never bothered me since none of them had a clue as to what a histotech did. When they removed the histology rotation from the MT training in the early 70's they didn't have any exposure to our world. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Tuesday, May 20, 2008 2:45 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect Joelle, Best pathologist quote that I've ever heard myself: "Let's face it, a trained monkey can cut blocks." This was referring to trying to staff his personal research lab and was said with all seriousness. Needless to say, this pathologist and I didn't get along well at all. Unfortunately, too many share these sentiments and don't have a clue that a histotech tends to do a bit more than "cut blocks". Sort of makes you wonder why any of us bothers with getting certified or with obtaining necessary CEU's to stay that way. Hang in There, Glen Dawson BS, HT & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of joelle weaver Sent: Tuesday, May 20, 2008 12:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] histotech pay/respect In response to several postings regarding pay and respect- I have been feeling somewhat better to read that others are disheartened by the lack of respect for the effort and time that it can take to become a certified, experienced histotech. The pay doesn't really bother me as much as the attitude that I encounter on a daily basis that "anyone" can do histology and that it is to quote "no big deal" to get certified. I have to concur that in my experience, histology managers do not seem to value or even recognize the skills and time it takes to perfect this trade.I know for me, getting an HTL was quite a burden at times. I had extra studies for sure. And, back in that day, the ASCP slide practical was no picnic. At my own hospital, this attitude is epitomized by the fact that routinely non-certifed, non-histology people are both hired and promoted. I have never seen anyone with a histology background given any sort of professional respect like that given freely to the MT personnel that work there. They do not even recgonize an HTL as a certification. They only give you a small increase for being certified at all (HT). At particularly bad moments, this had made me want to leave the field as well. So, I can relate very easily to everyone else's sentiments. This hospital has been an especially bad example of the "warm body" syndrome. It is almost crippling in terms of the quality of the work and TAT. The inferior service and poor quality produced only serves to reinforce the negative concept- it is indeed a vicious cycle! At least (all of us) are not alone! Joelle _________________________________________________________________ Keep your kids safer online with Windows Live Family Safety. http://www.windowslive.com/family_safety/overview.html?ocid=TXT_TAGLM_WL _Refresh_family_safety_052008___________________________________________ ____ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed May 21 09:57:01 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed May 21 09:58:51 2008 Subject: [Histonet] histotech pay/respect In-Reply-To: <5C0BED61F529364E86309CADEA63FEF20163F3FF@TRFT-EX01.xRothGen.nhs.uk> References: <5C0BED61F529364E86309CADEA63FEF20163F3FF@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A747A398@LTA3VS011.ees.hhs.gov> Shame on you! LOL! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: Wednesday, May 21, 2008 10:17 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect Gee, the number of times I would liked to have done that:-) Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 21 May 2008 15:03 To: Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect I have to say that the majority of pathologists I have worked with over the years have treated me with respect but not all. And the ones that did not treat me respectfully did not treat any of the laboratorians with respect. So I can't say respect is always earned. There are often a few bad eggs in the mix. I can remember as a student (back in the day) one of the pathologists came into the lab with a tray of H&E's and shouted, "Who cut this sh**?!" Then he threw the tray on the floor and broke all of the slides. Now I am sure that type of behavior would never be allowed today. But honestly, over the many years I have been in histology I can say only 2 have been truly disrespectful. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Wednesday, May 21, 2008 9:26 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect Sorry Ren? but I don't think having worked with 12 different pathologists and being treated with respect by ALL of them is a matter of good luck. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com ________________________________ From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Wednesday, May 21, 2008 9:09 AM To: Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect Having a good experience with a pathologist (or even with several) is a sign of "good luck", like ending with a good spouse. We histotech should not be subjected to the "good (or bad) whims of pathologists", it should be a RIGHT to earn decent salaries and be treated with respect. I am sure that during slavery times, there were bad and "not so bad" plantation owners! Ren? J. "Blazek, Linda" wrote: Patti and all, Horary Patti. I was just sitting here getting ready to post my email when you posted yours. I was beginning to think I was the only one here that has had a very positive experience as a histotech. I've been watching this topic for a long time now and wonder where I've been. I've been a histotech since the early 70's. The pathologist I worked for in the early 80's wanted to know why I never took my registry when I finished school. I guess a baby got in the way and no one really required it. He pushed and encouraged me to take my registry. He was so supportive and helpful to someone that waited 9 years to sit for the exam that it was amazing. I did receive a healthy raise when I passed. I have never in all these years worked for a pathologist that wasn't respectful and included me as a part of a team. I have had a bit of "red-headed stepchild" reactions from MTs but that never bothered me since none of them had a clue as to what a histotech did. When they removed the histology rotation from the MT training in the early 70's they didn't have any exposure to our world. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Tuesday, May 20, 2008 2:45 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect Joelle, Best pathologist quote that I've ever heard myself: "Let's face it, a trained monkey can cut blocks." This was referring to trying to staff his personal research lab and was said with all seriousness. Needless to say, this pathologist and I didn't get along well at all. Unfortunately, too many share these sentiments and don't have a clue that a histotech tends to do a bit more than "cut blocks". Sort of makes you wonder why any of us bothers with getting certified or with obtaining necessary CEU's to stay that way. Hang in There, Glen Dawson BS, HT & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of joelle weaver Sent: Tuesday, May 20, 2008 12:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] histotech pay/respect In response to several postings regarding pay and respect- I have been feeling somewhat better to read that others are disheartened by the lack of respect for the effort and time that it can take to become a certified, experienced histotech. The pay doesn't really bother me as much as the attitude that I encounter on a daily basis that "anyone" can do histology and that it is to quote "no big deal" to get certified. I have to concur that in my experience, histology managers do not seem to value or even recognize the skills and time it takes to perfect this trade.I know for me, getting an HTL was quite a burden at times. I had extra studies for sure. And, back in that day, the ASCP slide practical was no picnic. At my own hospital, this attitude is epitomized by the fact that routinely non-certifed, non-histology people are both hired and promoted. I have never seen anyone with a histology background given any sort of professional respect like that given freely to the MT personnel that work there. They do not even recgonize an HTL as a certification. They only give you a small increase for being certified at all (HT). At particularly bad moments, this had made me want to leave the field as well. So, I can relate very easily to everyone else's sentiments. This hospital has been an especially bad example of the "warm body" syndrome. It is almost crippling in terms of the quality of the work and TAT. The inferior service and poor quality produced only serves to reinforce the negative concept- it is indeed a vicious cycle! At least (all of us) are not alone! Joelle _________________________________________________________________ Keep your kids safer online with Windows Live Family Safety. http://www.windowslive.com/family_safety/overview.html?ocid=TXT_TAGLM_WL _Refresh_family_safety_052008___________________________________________ ____ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Wed May 21 10:01:58 2008 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Wed May 21 10:02:14 2008 Subject: [Histonet] histotech pay/respect (private joke) Message-ID: <5C0BED61F529364E86309CADEA63FEF20163F400@TRFT-EX01.xRothGen.nhs.uk> What? Scotland is still in the UK? I thought England was a protectorate of Scotland. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian Montgomery Sent: 21 May 2008 15:44 To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] histotech pay/respect I've been following this discussion with interest as it's all so different here, we know our place. The master servant relationship is alive and well in the UK. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: 21 May 2008 15:17 To: Bartlett, Jeanine (CDC/CCID/NCZVED); Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect Gee, the number of times I would liked to have done that:-) Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 21 May 2008 15:03 To: Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect I have to say that the majority of pathologists I have worked with over the years have treated me with respect but not all. And the ones that did not treat me respectfully did not treat any of the laboratorians with respect. So I can't say respect is always earned. There are often a few bad eggs in the mix. I can remember as a student (back in the day) one of the pathologists came into the lab with a tray of H&E's and shouted, "Who cut this sh**?!" Then he threw the tray on the floor and broke all of the slides. Now I am sure that type of behavior would never be allowed today. But honestly, over the many years I have been in histology I can say only 2 have been truly disrespectful. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Wednesday, May 21, 2008 9:26 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect Sorry Ren? but I don't think having worked with 12 different pathologists and being treated with respect by ALL of them is a matter of good luck. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com ________________________________ From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Wednesday, May 21, 2008 9:09 AM To: Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect Having a good experience with a pathologist (or even with several) is a sign of "good luck", like ending with a good spouse. We histotech should not be subjected to the "good (or bad) whims of pathologists", it should be a RIGHT to earn decent salaries and be treated with respect. I am sure that during slavery times, there were bad and "not so bad" plantation owners! Ren? J. "Blazek, Linda" wrote: Patti and all, Horary Patti. I was just sitting here getting ready to post my email when you posted yours. I was beginning to think I was the only one here that has had a very positive experience as a histotech. I've been watching this topic for a long time now and wonder where I've been. I've been a histotech since the early 70's. The pathologist I worked for in the early 80's wanted to know why I never took my registry when I finished school. I guess a baby got in the way and no one really required it. He pushed and encouraged me to take my registry. He was so supportive and helpful to someone that waited 9 years to sit for the exam that it was amazing. I did receive a healthy raise when I passed. I have never in all these years worked for a pathologist that wasn't respectful and included me as a part of a team. I have had a bit of "red-headed stepchild" reactions from MTs but that never bothered me since none of them had a clue as to what a histotech did. When they removed the histology rotation from the MT training in the early 70's they didn't have any exposure to our world. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Tuesday, May 20, 2008 2:45 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect Joelle, Best pathologist quote that I've ever heard myself: "Let's face it, a trained monkey can cut blocks." This was referring to trying to staff his personal research lab and was said with all seriousness. Needless to say, this pathologist and I didn't get along well at all. Unfortunately, too many share these sentiments and don't have a clue that a histotech tends to do a bit more than "cut blocks". Sort of makes you wonder why any of us bothers with getting certified or with obtaining necessary CEU's to stay that way. Hang in There, Glen Dawson BS, HT & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of joelle weaver Sent: Tuesday, May 20, 2008 12:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] histotech pay/respect In response to several postings regarding pay and respect- I have been feeling somewhat better to read that others are disheartened by the lack of respect for the effort and time that it can take to become a certified, experienced histotech. The pay doesn't really bother me as much as the attitude that I encounter on a daily basis that "anyone" can do histology and that it is to quote "no big deal" to get certified. I have to concur that in my experience, histology managers do not seem to value or even recognize the skills and time it takes to perfect this trade.I know for me, getting an HTL was quite a burden at times. I had extra studies for sure. And, back in that day, the ASCP slide practical was no picnic. At my own hospital, this attitude is epitomized by the fact that routinely non-certifed, non-histology people are both hired and promoted. I have never seen anyone with a histology background given any sort of professional respect like that given freely to the MT personnel that work there. They do not even recgonize an HTL as a certification. They only give you a small increase for being certified at all (HT). At particularly bad moments, this had made me want to leave the field as well. So, I can relate very easily to everyone else's sentiments. This hospital has been an especially bad example of the "warm body" syndrome. It is almost crippling in terms of the quality of the work and TAT. The inferior service and poor quality produced only serves to reinforce the negative concept- it is indeed a vicious cycle! At least (all of us) are not alone! Joelle _________________________________________________________________ Keep your kids safer online with Windows Live Family Safety. http://www.windowslive.com/family_safety/overview.html?ocid=TXT_TAGLM_WL _Refresh_family_safety_052008___________________________________________ ____ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From integrated.histo <@t> gmail.com Wed May 21 10:25:42 2008 From: integrated.histo <@t> gmail.com (Cindy DuBois) Date: Wed May 21 10:25:46 2008 Subject: [Histonet] Working naked Message-ID: <5d9104a30805210825w3ce55398rc388f69d3750a214@mail.gmail.com> We have threatened to come to work in our bathing suits and run through the sprinklers during our breaks. Our lab manager insists he is working on the problem (I have worked for him for 20 years and I believe he is). The property owners claim they will only allow the A/C to be on for 12 hours a day and never on weekends and the pathologists want it on when they are here 7am - 7pm. Our lab manager sent an e-mail to the pathologist and said if a solution is not worked out by friday, we will not be coming in until 6 am (our start time now is 3:30). I have also printed out lots of material from the EPA about air exchange and proper storage of flammable materials. Hopefully something will convince the pathologists to fight the property owners on this. Meanwhile, I'll be coming to Albuquerque in June for the summer symposium and hopefully your temps will be cooler. Cindy From Ngale <@t> bccancer.bc.ca Wed May 21 10:40:00 2008 From: Ngale <@t> bccancer.bc.ca (Gale, Nadia) Date: Wed May 21 10:40:09 2008 Subject: [Histonet] Digital microscope camera Message-ID: I'm looking to adapt our lab microscope (Nikon Eclipse 50i) with a digital camera for publication quality photos that can be taken quickly. While I would love to purchase one of Nikon's cameras made for this purpose, we just don't have the budget. Does anyone have experience with setting up a consumer-level digital camera with a brightfield scope? As an aside, I'm disheartened to hear about the negative aspects of histotechnology in the States. In Canada, as in many other countries, histology is a division of the medical laboratory technology program and is treated with the same respect as any other discipline in the lab. Is there any lobby to have histology re-integrated into the general MLT programs? Regards, Nadia Nadia Gale Lead Histotechnologist Centre for Translational and Applied Genomics (CTAG) 3427-600 West 10th Avenue Vancouver, BC V5Z 4E6 tel 604-877-6000 ext 2426 fax 604-877-6089 ngale@bccancer.bc.ca From jqb7 <@t> cdc.gov Wed May 21 10:38:17 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed May 21 10:41:13 2008 Subject: [Histonet] Working naked In-Reply-To: <5d9104a30805210825w3ce55398rc388f69d3750a214@mail.gmail.com> References: <5d9104a30805210825w3ce55398rc388f69d3750a214@mail.gmail.com> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A747A39A@LTA3VS011.ees.hhs.gov> Those pesky pathologists...........are they are least treating you respectfully when they insist on A/C during their hours? LOL! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy DuBois Sent: Wednesday, May 21, 2008 11:26 AM To: sbreeden@nmda.nmsu.edu; Histonet Subject: [Histonet] Working naked We have threatened to come to work in our bathing suits and run through the sprinklers during our breaks. Our lab manager insists he is working on the problem (I have worked for him for 20 years and I believe he is). The property owners claim they will only allow the A/C to be on for 12 hours a day and never on weekends and the pathologists want it on when they are here 7am - 7pm. Our lab manager sent an e-mail to the pathologist and said if a solution is not worked out by friday, we will not be coming in until 6 am (our start time now is 3:30). I have also printed out lots of material from the EPA about air exchange and proper storage of flammable materials. Hopefully something will convince the pathologists to fight the property owners on this. Meanwhile, I'll be coming to Albuquerque in June for the summer symposium and hopefully your temps will be cooler. Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rchiovetti <@t> yahoo.com Wed May 21 11:01:49 2008 From: rchiovetti <@t> yahoo.com (Robert Chiovetti) Date: Wed May 21 11:01:55 2008 Subject: [Histonet] Digital microscope camera Message-ID: <485331.46636.qm@web58902.mail.re1.yahoo.com> Nadia, There are a couple of manufacturers that make adapters for the more popular "point-and-shoot" digital cameras. With the proper adapter you can remove an eyepiece, insert the adapter (with camera attached) into the eyepiece tube and take pretty decent quality digital photos. It helps a lot if your digital camera can be set to "infinity focus" (usually an icon with mountains) and if you can set the camera's exposure control to "aperture priority" or "shutter priority." The exact terminology might be different, depending on what kind of camera you have. You can use the camera's "zoom" settings to convert the circular field of view in the scope to a rectangular image for the camera. The adapters cost about as much as (or a little more than) a decent digital camera, but it's still less expensive than a dedicated microscope camera. I've had very good luck with the adapters made by Bobby Martin at Martin Microscope. Take a look at: www.martinmicroscope.com. Disclaimer: I have no personal or financial ties to Martin Microscope. I just sell their stuff on occasion. Good luck! Cheers, Bob Chiovetti Robert (Bob) Chiovetti, Ph.D. Southwest Precision Instruments See What's New on Our Website! Arizona's Microscopy Resource 132 North Elster Drive Tucson, AZ 85710-3212 Tel./Fax 520-546-4986 Member, Arizona Small Business Association (www.asba.com) ----- Original Message ---- From: "Gale, Nadia" To: histonet@lists.utsouthwestern.edu Sent: Wednesday, May 21, 2008 9:40:00 AM Subject: [Histonet] Digital microscope camera I'm looking to adapt our lab microscope (Nikon Eclipse 50i) with a digital camera for publication quality photos that can be taken quickly. While I would love to purchase one of Nikon's cameras made for this purpose, we just don't have the budget. Does anyone have experience with setting up a consumer-level digital camera with a brightfield scope? As an aside, I'm disheartened to hear about the negative aspects of histotechnology in the States. In Canada, as in many other countries, histology is a division of the medical laboratory technology program and is treated with the same respect as any other discipline in the lab. Is there any lobby to have histology re-integrated into the general MLT programs? Regards, Nadia Nadia Gale Lead Histotechnologist Centre for Translational and Applied Genomics (CTAG) 3427-600 West 10th Avenue Vancouver, BC V5Z 4E6 tel 604-877-6000 ext 2426 fax 604-877-6089 ngale@bccancer.bc.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ktuttle <@t> umm.edu Wed May 21 11:27:42 2008 From: ktuttle <@t> umm.edu (Kimberly Tuttle) Date: Wed May 21 11:28:08 2008 Subject: [Histonet] S100P on paraffin References: <47D7E165.90CE.001A.3@umm.edu> Message-ID: <4834153D.90CE.001A.3@umm.edu> Has anyone has success with S100P on paraffin embedded tissue. I followed the protocol exactly as published in Am J Clin Path. We got cytoplasmic staining but no nuclear staining- therefore negative. Suggestions? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From Rcartun <@t> harthosp.org Wed May 21 11:28:53 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed May 21 11:29:06 2008 Subject: [Histonet] Polyomavirus Message-ID: <483415850200007700002BBE@gwmail6.harthosp.org> Is anyone using a monoclonal anti-polyomavirus antibody (clone PAb101) from BD Pharmingen in IHC staining? If so, please contact me. Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From tkngflght <@t> yahoo.com Wed May 21 11:52:14 2008 From: tkngflght <@t> yahoo.com (Cheryl) Date: Wed May 21 11:52:14 2008 Subject: [Histonet] Working naked In-Reply-To: <1CE1847DFEA0A647B1CCDE4108EA60A747A39A@LTA3VS011.ees.hhs.gov> References: <5d9104a30805210825w3ce55398rc388f69d3750a214@mail.gmail.com> <1CE1847DFEA0A647B1CCDE4108EA60A747A39A@LTA3VS011.ees.hhs.gov> Message-ID: <00d301c8bb63$060e3550$400aa8c0@FSROGER> I've been known to sport and use a squirt gun. HR said as long as I applied it uniformly across all employee/job descriptions and didn't show favoritism or use it as a threat, it was okay :) Cheryl Cheryl R. Kerry, HT(ASCP), BA Full Staff Inc. Staffing the lab - One GREAT tech at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone admin@fullstaff.org www.fullstaff.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Wednesday, May 21, 2008 10:38 AM To: Cindy DuBois; sbreeden@nmda.nmsu.edu; Histonet Subject: RE: [Histonet] Working naked Those pesky pathologists...........are they are least treating you respectfully when they insist on A/C during their hours? LOL! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy DuBois Sent: Wednesday, May 21, 2008 11:26 AM To: sbreeden@nmda.nmsu.edu; Histonet Subject: [Histonet] Working naked We have threatened to come to work in our bathing suits and run through the sprinklers during our breaks. Our lab manager insists he is working on the problem (I have worked for him for 20 years and I believe he is). The property owners claim they will only allow the A/C to be on for 12 hours a day and never on weekends and the pathologists want it on when they are here 7am - 7pm. Our lab manager sent an e-mail to the pathologist and said if a solution is not worked out by friday, we will not be coming in until 6 am (our start time now is 3:30). I have also printed out lots of material from the EPA about air exchange and proper storage of flammable materials. Hopefully something will convince the pathologists to fight the property owners on this. Meanwhile, I'll be coming to Albuquerque in June for the summer symposium and hopefully your temps will be cooler. Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Wed May 21 12:12:17 2008 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Wed May 21 12:14:18 2008 Subject: [Histonet] Working naked References: <5d9104a30805210825w3ce55398rc388f69d3750a214@mail.gmail.com><1CE1847DFEA0A647B1CCDE4108EA60A747A39A@LTA3VS011.ees.hhs.gov> <00d301c8bb63$060e3550$400aa8c0@FSROGER> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F267F@fhosxchmb006.ADVENTISTCORP.NET> I remember the xylene evaporating at a rapid rate at those temperatures and the Pathologists would have to send us home, we were acting a little "funny". (At least more than normal) Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cheryl Sent: Wed 5/21/2008 12:52 PM To: 'Histonet' Subject: RE: [Histonet] Working naked I've been known to sport and use a squirt gun. HR said as long as I applied it uniformly across all employee/job descriptions and didn't show favoritism or use it as a threat, it was okay :) Cheryl Cheryl R. Kerry, HT(ASCP), BA Full Staff Inc. Staffing the lab - One GREAT tech at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone admin@fullstaff.org www.fullstaff.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Wednesday, May 21, 2008 10:38 AM To: Cindy DuBois; sbreeden@nmda.nmsu.edu; Histonet Subject: RE: [Histonet] Working naked Those pesky pathologists...........are they are least treating you respectfully when they insist on A/C during their hours? LOL! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy DuBois Sent: Wednesday, May 21, 2008 11:26 AM To: sbreeden@nmda.nmsu.edu; Histonet Subject: [Histonet] Working naked We have threatened to come to work in our bathing suits and run through the sprinklers during our breaks. Our lab manager insists he is working on the problem (I have worked for him for 20 years and I believe he is). The property owners claim they will only allow the A/C to be on for 12 hours a day and never on weekends and the pathologists want it on when they are here 7am - 7pm. Our lab manager sent an e-mail to the pathologist and said if a solution is not worked out by friday, we will not be coming in until 6 am (our start time now is 3:30). I have also printed out lots of material from the EPA about air exchange and proper storage of flammable materials. Hopefully something will convince the pathologists to fight the property owners on this. Meanwhile, I'll be coming to Albuquerque in June for the summer symposium and hopefully your temps will be cooler. Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From jkiernan <@t> uwo.ca Wed May 21 12:23:42 2008 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed May 21 12:23:49 2008 Subject: [Histonet] Digital microscope camera In-Reply-To: <485331.46636.qm@web58902.mail.re1.yahoo.com> References: <485331.46636.qm@web58902.mail.re1.yahoo.com> Message-ID: Look up Motic Images on the Web. They sell cameras with a set of adapters for eyepiece, C-mount etc. I bought one about 4 years ago for about $300. It will take still pictures or movies up to about 30 seconds - good for living protozoa that won't keep still for you to have a good look at them. The software that comes with the Motic camera isn't compatible with Windows Vista, unfortunately, but I expect they'll be attending to that. John Kiernan London, Canada = = = > ----- Original Message ---- > From: "Gale, Nadia" > To: histonet@lists.utsouthwestern.edu > Sent: Wednesday, May 21, 2008 9:40:00 AM > Subject: [Histonet] Digital microscope camera > > I'm looking to adapt our lab microscope (Nikon Eclipse 50i) with a > digital camera for publication quality photos that can be taken > quickly.While I would love to purchase one of Nikon's cameras > made for this > purpose, we just don't have the budget. Does anyone have > experiencewith setting up a consumer-level digital camera with a > brightfieldscope? > > As an aside, I'm disheartened to hear about the negative aspects of > histotechnology in the States. In Canada, as in many other > countries,histology is a division of the medical laboratory > technology program and > is treated with the same respect as any other discipline in the > lab. Is > there any lobby to have histology re-integrated into the general MLT > programs? > > > Regards, > Nadia > > Nadia Gale > Lead Histotechnologist > Centre for Translational and Applied Genomics (CTAG) > 3427-600 West 10th Avenue > Vancouver, BC V5Z 4E6 > tel 604-877-6000 ext 2426 > fax 604-877-6089 > ngale@bccancer.bc.ca > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From octavio109 <@t> hotmail.com Wed May 21 13:38:01 2008 From: octavio109 <@t> hotmail.com (Corinthia D. Emanuel) Date: Wed May 21 13:38:06 2008 Subject: [Histonet] Histotechs needed in Atlanta, Ga In-Reply-To: References: Message-ID: Fax resume to 770-381-6451 for immediate consideration. Sign on bonus of $1000! Experienced ONLY in cutting, staining, grossing and embedding, please! Certification preferred, but not required. Full-time and part-time postions available. All shifts. _________________________________________________________________ Change the world with e-mail. Join the i?m Initiative from Microsoft. http://im.live.com/Messenger/IM/Join/Default.aspx?source=EML_WL_ChangeWorld From hej01 <@t> health.state.ny.us Wed May 21 13:50:34 2008 From: hej01 <@t> health.state.ny.us (Helen E Johnson) Date: Wed May 21 13:50:42 2008 Subject: [Histonet] staining cell cultures with Giemsa Message-ID: Hi Histonetters, Does anyone have a protocol staining cell cultures with Giemsa? Thanks. Helen Johnson (hej01@health.state.ny.us) IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. From akbitting <@t> geisinger.edu Wed May 21 14:18:02 2008 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Wed May 21 14:18:16 2008 Subject: [Histonet] Richard Allan Hematoxylin problems In-Reply-To: <007701c8bab4$2e2d27d0$6501a8c0@DHXTS541> References: <80ab7bc60805201220h44cb0e54y8223f5231a7fc495@mail.gmail.com> <007701c8bab4$2e2d27d0$6501a8c0@DHXTS541> Message-ID: <48343D2A.2B7F.00C9.0@geisinger.edu> We have always changed our hematoxylin every Sunday night, and just filtered on the days in between. We've had no change in volumes. Now, all of a sudden, by Tuesday my hematoxylin staining is so pale that the docs are screaming. This has been going on for about two weeks now. We use Richard Allan Modified Harris Hematoxylin. Anyone else seen a change with that brand? I know there is supposed to be a "shortage" of hematoxylin right now. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> "Gayle Callis" 5/20/2008 4:00 PM >>> We use xylene to remove coverslips and do this work inside a hood. After coverslip is off and mounting media is gone with an extra xylene rinse, we go back to Clearite 3 to rinse off xylene and recoverslip. Anything to try an minimize exposure to xylene is done. Unfortunately, we found the substitute just didn't work for this job. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT ----- Original Message ----- From: "Patty Dunlop" To: Sent: Tuesday, May 20, 2008 1:20 PM Subject: [Histonet] xylene substitute recoverslipping > Hello Histonetters, > > I use Clear-Rite 3, a xylene substitute, and am having problems when I > need > to recoverslip. Currently, I just put the slide that needs recoverslipped > into a coplin jar of Clear-rite 3 at room temperature. The coverslip > really > doesn't like to come off (takes a week) and it tends to be a sticky, messy > process. Does anyone have a method for removing coverslips with a xylene > substitute? Should I use heat? > > Thanks, > Patty > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From b-frederick <@t> northwestern.edu Wed May 21 14:41:43 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed May 21 14:41:53 2008 Subject: [Histonet] Working naked In-Reply-To: <00d301c8bb63$060e3550$400aa8c0@FSROGER> Message-ID: <000001c8bb7a$b2d978a0$d00f7ca5@lurie.northwestern.edu> I worked in a lab where embeddinf cold plates were used instead of ice trays. Those having hot flashes loved them- laid there heads on them!!!! Barring that,there is always the -80 freezer (instant FS!) Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Wednesday, May 21, 2008 11:52 AM To: 'Histonet' Subject: RE: [Histonet] Working naked I've been known to sport and use a squirt gun. HR said as long as I applied it uniformly across all employee/job descriptions and didn't show favoritism or use it as a threat, it was okay :) Cheryl Cheryl R. Kerry, HT(ASCP), BA Full Staff Inc. Staffing the lab - One GREAT tech at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone admin@fullstaff.org www.fullstaff.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Wednesday, May 21, 2008 10:38 AM To: Cindy DuBois; sbreeden@nmda.nmsu.edu; Histonet Subject: RE: [Histonet] Working naked Those pesky pathologists...........are they are least treating you respectfully when they insist on A/C during their hours? LOL! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy DuBois Sent: Wednesday, May 21, 2008 11:26 AM To: sbreeden@nmda.nmsu.edu; Histonet Subject: [Histonet] Working naked We have threatened to come to work in our bathing suits and run through the sprinklers during our breaks. Our lab manager insists he is working on the problem (I have worked for him for 20 years and I believe he is). The property owners claim they will only allow the A/C to be on for 12 hours a day and never on weekends and the pathologists want it on when they are here 7am - 7pm. Our lab manager sent an e-mail to the pathologist and said if a solution is not worked out by friday, we will not be coming in until 6 am (our start time now is 3:30). I have also printed out lots of material from the EPA about air exchange and proper storage of flammable materials. Hopefully something will convince the pathologists to fight the property owners on this. Meanwhile, I'll be coming to Albuquerque in June for the summer symposium and hopefully your temps will be cooler. Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From plucas <@t> biopath.org Wed May 21 15:10:22 2008 From: plucas <@t> biopath.org (Paula Lucas) Date: Wed May 21 15:10:29 2008 Subject: [Histonet] Richard Allan Hematoxylin problems In-Reply-To: <80ab7bc60805201220h44cb0e54y8223f5231a7fc495@mail.gmail.com> <007701c8bab4$2e2d27d0$6501a8c0@DHXTS541> Message-ID: <20080521201022.D9119D2AEB@swiftsure.cnchost.com> Yes, I am experiencing the same problem. I haven't changed my protocol, since it's been in place now for the past 6 years. About three weeks ago, my pathologist started complaining that the nuclei is too pale. I use their 7211 and I asked for a sample of their Hematoxylin 1. I'll see if it produces a darker stain. Paula ---- Angela Bitting wrote: > > We have always changed our hematoxylin every Sunday night, and just filtered on the days in between. We've had no change in volumes. Now, all of a sudden, by Tuesday my hematoxylin staining is so pale that the docs are screaming. This has been going on for about two weeks now. We use Richard Allan Modified Harris Hematoxylin. Anyone else seen a change with that brand? I know there is supposed to be a "shortage" of hematoxylin right now. > > Angela Bitting, HT(ASCP) > Technical Specialist, Histology > Geisinger Medical Center > 100 N Academy Ave. MC 23-00 > Danville, PA 17822 > phone 570-214-9634 > fax 570-271-5916 > > No trees were hurt in the sending of this email > However many electrons were severly inconvienienced! > > > >>> "Gayle Callis" 5/20/2008 4:00 PM >>> > We use xylene to remove coverslips and do this work inside a hood. After > coverslip is off and mounting media is gone with an extra xylene rinse, we > go back to Clearite 3 to rinse off xylene and recoverslip. Anything to try > an minimize exposure to xylene is done. Unfortunately, we found the > substitute just didn't work for this job. > > Gayle M. Callis > HTL/HT/MT(ASCP) > Bozeman MT > > > ----- Original Message ----- > From: "Patty Dunlop" > To: > Sent: Tuesday, May 20, 2008 1:20 PM > Subject: [Histonet] xylene substitute recoverslipping > > > > Hello Histonetters, > > > > I use Clear-Rite 3, a xylene substitute, and am having problems when I > > need > > to recoverslip. Currently, I just put the slide that needs recoverslipped > > into a coplin jar of Clear-rite 3 at room temperature. The coverslip > > really > > doesn't like to come off (takes a week) and it tends to be a sticky, messy > > process. Does anyone have a method for removing coverslips with a xylene > > substitute? Should I use heat? > > > > Thanks, > > Patty > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From rjbuesa <@t> yahoo.com Wed May 21 15:31:15 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 21 15:31:20 2008 Subject: [Histonet] removing tissue from already H and E stained slides. In-Reply-To: Message-ID: <31399.94853.qm@web65707.mail.ac4.yahoo.com> There is no REALLY any good way to do that (even with some special adherents that solidify over them). It would be better to make an hydrophobic barrier between the two sections and stain one with ER and the other with PR. Ren? J. AMH-HistoLab wrote: From LeslieS <@t> vetmed.ufl.edu Wed May 21 15:30:59 2008 From: LeslieS <@t> vetmed.ufl.edu (Shawn Leslie) Date: Wed May 21 15:31:24 2008 Subject: [Histonet] Richard Allan Hematoxylin problems In-Reply-To: <48343D2A.2B7F.00C9.0@geisinger.edu> References: <80ab7bc60805201220h44cb0e54y8223f5231a7fc495@mail.gmail.com> <007701c8bab4$2e2d27d0$6501a8c0@DHXTS541> <48343D2A.2B7F.00C9.0@geisinger.edu> Message-ID: <48344E43.CC0E.007B.0@vetmed.ufl.edu> We also use the Richard Allen 7211 but we haven't had any complaints. Maybe it's just a bad batch. Contact the vendor I am sure they will fix it.... Shawn Leslie HT (ASCP) Scientific Research Manager Anatomic Pathology University of Florida School of Veterinary Medicine 352-392-2235 ext 4555 >>> "Angela Bitting" 5/21/2008 3:18 PM >>> We have always changed our hematoxylin every Sunday night, and just filtered on the days in between. We've had no change in volumes. Now, all of a sudden, by Tuesday my hematoxylin staining is so pale that the docs are screaming. This has been going on for about two weeks now. We use Richard Allan Modified Harris Hematoxylin. Anyone else seen a change with that brand? I know there is supposed to be a "shortage" of hematoxylin right now. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> "Gayle Callis" 5/20/2008 4:00 PM >>> We use xylene to remove coverslips and do this work inside a hood. After coverslip is off and mounting media is gone with an extra xylene rinse, we go back to Clearite 3 to rinse off xylene and recoverslip. Anything to try an minimize exposure to xylene is done. Unfortunately, we found the substitute just didn't work for this job. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT ----- Original Message ----- From: "Patty Dunlop" To: Sent: Tuesday, May 20, 2008 1:20 PM Subject: [Histonet] xylene substitute recoverslipping > Hello Histonetters, > > I use Clear-Rite 3, a xylene substitute, and am having problems when I > need > to recoverslip. Currently, I just put the slide that needs recoverslipped > into a coplin jar of Clear-rite 3 at room temperature. The coverslip > really > doesn't like to come off (takes a week) and it tends to be a sticky, messy > process. Does anyone have a method for removing coverslips with a xylene > substitute? Should I use heat? > > Thanks, > Patty > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From jennifer.l.hofecker <@t> Vanderbilt.Edu Wed May 21 15:32:19 2008 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Wed May 21 15:32:25 2008 Subject: [Histonet] Users of Ini-1 Message-ID: <898D946569A27444B65667A49C0740520175B4CA@mailbe06.mc.vanderbilt.edu> Hi everyone, Are any of you using Ini-1 on formalin fixed paraffin embedded (human) brain tumors? If you wouldn't mind, please contact me off - list. I would like to discuss optimization of the antibody with other users. I am currently using Santa Cruz rabbit poly (H-300). I am especially interested in experiences with this particular product. Thanks so much! Jennifer L. Hofecker HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph 615.343.0083 fax 615.343.7089 From ploykasek <@t> phenopath.com Wed May 21 15:42:26 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Wed May 21 15:42:38 2008 Subject: FW: [Histonet] removing tissue from already H and E stained slides. In-Reply-To: Message-ID: ------ Forwarded Message From: Patti Loykasek Date: Wed, 21 May 2008 09:07:30 -0700 To: AMH-HistoLab Subject: Re: [Histonet] removing tissue from already H and E stained slides. Is your pretreatment for ER & PR the same? If so, there's no need to remove a section. The slide can be stained manually. Simply pretreat the slide (with appropriate controls of course), use a barrier pen around each section, carefully apply each antibody - 1 per section- & perform your detection etc.. as usual. If your H&E is on a non-adhesive slide you do run the risk of losing your sections during pretreatment. Hope this helps. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > I have a situation where a pathologist needs an Er and PR IHC done on a > previous H and E stained slide. The slide has two sections on it and she > asked if one section can be removed to another slide to perform ER on > one slide and PR on the other. Does anyone know of a solution that will > remove tissue safely from a slide after H and E stained and not > interfere with IHC? > > Abington Memorial Hospital > 1200 Old York Rd > Abington, PA. 19001 > > > > ****************** CONFIDENTIALITY NOTICE ********************** > > This e-mail contains LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION > intended only for the use of the recipient named above. If you are not > the intended recipient, you are hereby notified that any dissemination or > copying of this e-mail is strictly prohibited. If you have received this > e-mail in error, please notify the transmitting hospital by telephone or > e-mail and delete the original e-mail received in error. > > THIS INFORMATION HAS BEEN DISCLOSED TO YOU FROM RECORDS WHOSE > CONFIDENTIALITY IS PROTECTED BY STATE AND FEDERAL LAW. ANY FURTHER > DISCLOSURE, COPYING, DISTRIBUTION OR ACTION TAKEN IN RELIANCE ON THE > CONTENTS OF THESE DOCUMENTS WITHOUT THE PRIOR WRITTEN CONSENT OF THE > PERSON TO WHOM IT PERTAINS IS PROHIBITED. YOU ARE REQUIRED TO DESTROY > THE INFORMATION AFTER THE STATED NEED HAS BEEN FULFILLED. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------ End of Forwarded Message This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From jnocito <@t> satx.rr.com Wed May 21 17:01:57 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed May 21 17:02:03 2008 Subject: [Histonet] histotech pay/respect References: <898D946569A27444B65667A49C0740520175B4C0@mailbe06.mc.vanderbilt.edu> Message-ID: <005f01c8bb8e$4a0830a0$0302a8c0@yourxhtr8hvc4p> Unlike Jenn, I have worked (emphasis on "worked") for some real doozies. Even though I was a manager of a private lab, I was by a partner that "she" was the owner. I told her that I've been with this lab longer than you and never did any of her partners bring that to my attention. I was also told that a pathologist's time was more valuable than mine, so when a problem surfaced that concerned him, I told him that I didn't have the time. To me, money isn't everything. I'd rather work in a place that had mutual respect, a nice atmosphere and friendly people. JTT ----- Original Message ----- From: "Hofecker, Jennifer L" To: "histonet" Sent: Wednesday, May 21, 2008 9:37 AM Subject: RE: [Histonet] histotech pay/respect I would also like to "chime in" and say that throughout my career, I have been very blessed to work with wonderful pathologists. The majority of them supported and encouraged my professional development. Unfortunately much of the time, their hands are tied when it comes to salaries. I have learned that although money is nice, it is quite rewarding to work with people who respect me and value my contributions to patient care. I agree, there have been a few "bad apples" who did not treat me with respect. That's when it's time to polish up the resume. Heading into the bunker now... Jennifer L. Hofecker HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph 615.343.0083 fax 615.343.7089 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Wednesday, May 21, 2008 9:17 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect Gee, the number of times I would liked to have done that:-) Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: 21 May 2008 15:03 To: Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect I have to say that the majority of pathologists I have worked with over the years have treated me with respect but not all. And the ones that did not treat me respectfully did not treat any of the laboratorians with respect. So I can't say respect is always earned. There are often a few bad eggs in the mix. I can remember as a student (back in the day) one of the pathologists came into the lab with a tray of H&E's and shouted, "Who cut this sh**?!" Then he threw the tray on the floor and broke all of the slides. Now I am sure that type of behavior would never be allowed today. But honestly, over the many years I have been in histology I can say only 2 have been truly disrespectful. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Wednesday, May 21, 2008 9:26 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect Sorry Ren? but I don't think having worked with 12 different pathologists and being treated with respect by ALL of them is a matter of good luck. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com ________________________________ From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Wednesday, May 21, 2008 9:09 AM To: Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect Having a good experience with a pathologist (or even with several) is a sign of "good luck", like ending with a good spouse. We histotech should not be subjected to the "good (or bad) whims of pathologists", it should be a RIGHT to earn decent salaries and be treated with respect. I am sure that during slavery times, there were bad and "not so bad" plantation owners! Ren? J. "Blazek, Linda" wrote: Patti and all, Horary Patti. I was just sitting here getting ready to post my email when you posted yours. I was beginning to think I was the only one here that has had a very positive experience as a histotech. I've been watching this topic for a long time now and wonder where I've been. I've been a histotech since the early 70's. The pathologist I worked for in the early 80's wanted to know why I never took my registry when I finished school. I guess a baby got in the way and no one really required it. He pushed and encouraged me to take my registry. He was so supportive and helpful to someone that waited 9 years to sit for the exam that it was amazing. I did receive a healthy raise when I passed. I have never in all these years worked for a pathologist that wasn't respectful and included me as a part of a team. I have had a bit of "red-headed stepchild" reactions from MTs but that never bothered me since none of them had a clue as to what a histotech did. When they removed the histology rotation from the MT training in the early 70's they didn't have any exposure to our world. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Tuesday, May 20, 2008 2:45 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histotech pay/respect Joelle, Best pathologist quote that I've ever heard myself: "Let's face it, a trained monkey can cut blocks." This was referring to trying to staff his personal research lab and was said with all seriousness. Needless to say, this pathologist and I didn't get along well at all. Unfortunately, too many share these sentiments and don't have a clue that a histotech tends to do a bit more than "cut blocks". Sort of makes you wonder why any of us bothers with getting certified or with obtaining necessary CEU's to stay that way. Hang in There, Glen Dawson BS, HT & QIHC IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of joelle weaver Sent: Tuesday, May 20, 2008 12:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] histotech pay/respect In response to several postings regarding pay and respect- I have been feeling somewhat better to read that others are disheartened by the lack of respect for the effort and time that it can take to become a certified, experienced histotech. The pay doesn't really bother me as much as the attitude that I encounter on a daily basis that "anyone" can do histology and that it is to quote "no big deal" to get certified. I have to concur that in my experience, histology managers do not seem to value or even recognize the skills and time it takes to perfect this trade.I know for me, getting an HTL was quite a burden at times. I had extra studies for sure. And, back in that day, the ASCP slide practical was no picnic. At my own hospital, this attitude is epitomized by the fact that routinely non-certifed, non-histology people are both hired and promoted. I have never seen anyone with a histology background given any sort of professional respect like that given freely to the MT personnel that work there. They do not even recgonize an HTL as a certification. They only give you a small increase for being certified at all (HT). At particularly bad moments, this had made me want to leave the field as well. So, I can relate very easily to everyone else's sentiments. This hospital has been an especially bad example of the "warm body" syndrome. It is almost crippling in terms of the quality of the work and TAT. The inferior service and poor quality produced only serves to reinforce the negative concept- it is indeed a vicious cycle! At least (all of us) are not alone! Joelle _________________________________________________________________ Keep your kids safer online with Windows Live Family Safety. http://www.windowslive.com/family_safety/overview.html?ocid=TXT_TAGLM_WL _Refresh_family_safety_052008___________________________________________ ____ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr <@t> comcast.net Wed May 21 23:53:53 2008 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Wed May 21 23:54:00 2008 Subject: [Histonet] staining cell cultures with Giemsa Message-ID: <052220080453.2915.4834FC610006174F00000B6322070029539D09020704040A0105@comcast.net> Helen, What "kind" of cell cultures? Primary bone marrow cultures? Immortal cell culture? Other primary lines? Why Giemsa? And grown in what? T-25's, T-150's? Or on chambered glass slides? Chambered glass slides? Have done Giemsa and many other histologic stains on "cell cultures" but the protocols (H&E, Giemsa, Oil Red O, etc) didn't vary much from standard histology lab other than I just used some common sense in adapting the particular situation to the stain. A primary bone marrow culture in a T150 flask, I would wash and "fix" the cells (methanol) for Giemsa. Other fixative for other stain. If I didn't mind viewing through the 2 flat sides, just do the stain in flask, emptying and washing. Or if I wanted to get a "close" view with higher power, after fixation saw through the "top" "flat" half, do stain in what was an open container. Or if I wanted really high power, I'd put sterile coverslips in the flask before seeding cells, grow to a monolayer, remove coverslips, fix and s tain them. Depends on the pickiness of the cell line. Or grow culture in chambered glass slides, remove chamber material, fix, stain. I guess my point is you might not need to look for a specific Giemsa modification (or histologic stain) for "cell cultures". You need to follow basic staining protocols and use common sense to adapt the stain to your very particular siutuation with type of cell and culture environment taken into consideration and not do something unwise (take culture flask through cyclic hydrocarbons like xylene to clear). This is all time consuming and sounds like a lot of work but common sense for your particular project unique to you will take you further than someone elses directions. Ray Koelling PhenoPath Labs Seattle, WA -------------- Original message -------------- From: Helen E Johnson > > Hi Histonetters, > Does anyone have a protocol staining cell cultures with Giemsa? > Thanks. Helen Johnson > (hej01@health.state.ny.us) > > > IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or > sensitive information which is, or may be, legally privileged or otherwise > protected by law from further disclosure. It is intended only for the > addressee. If you received this in error or from someone who was not authorized > to send it to you, please do not distribute, copy or use it or any attachments. > Please notify the sender immediately by reply e-mail and delete this from your > system. Thank you for your cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nfournier <@t> sasktel.net Thu May 22 01:14:01 2008 From: nfournier <@t> sasktel.net (Neil Fournier) Date: Thu May 22 01:12:09 2008 Subject: [Histonet] rat perfusion Message-ID: <000801c8bbd3$07ab0080$07d86e40@NEIL> Hi everyone, I was hoping that someone might have an answer that one of my colleagues is seeing during their rat perfusions. They have noticed that rats are making considerable movements during saline rinse. This is influencing the quality of perfusion with paraformaldehyde as the animals no longer make any movements and brain tissue (which we are interested in) appears quite soft. The issue is definitely not related to anesthetic as the animals are well over-dosed at this point and have all shown stage III anesthesia (we aren't barbaric here). Rats are initially intracardially perfused with 0.9% physiological saline (dissolved dH2O) to washout blood, followed by 4% paraformaldehyde (dissolved in 0.1 M PB) to fix tissue. Needle is placed in the ascending aorta or left ventricle and the heart clamped to keep needle in place, the right atrium is clipped. I have only two ideas regarding what the problem could be 1) related to salt content of the saline; or 2) some how the students are mispositioning the placement of the perfusion needle? Does anyone have an idea regarding what the problem could be? Has anyone seen this before? Neil From lpwenk <@t> sbcglobal.net Thu May 22 04:27:23 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu May 22 04:27:30 2008 Subject: [Histonet] Formaldehyde + Acetic Acid In-Reply-To: <901731.78152.qm@web65708.mail.ac4.yahoo.com> Message-ID: <000301c8bbee$0b0ea9a0$35ee2d4b@HPPav2> Thanks for the nice words, but unfortunately, my answer was based on my own real life error. Some years ago, I was making alcoholic formalin for the tissue processor. I had already mixed the alcohol and water, then poured in the formaldehyde. And watched it turn cloudy milky white, to my utter amazement. My thoughts? - it's never done that before - I wonder if it will go away if I wait a couple of minutes (no) - I wonder if it will go away if I swirl the container and mix the solutions better (no) - I wonder what's wrong with the water today (no idea why I thought the water was the problem) Finally, I thought about looking at the labels on the solutions, and found that I had inadvertantly grabbed the 10% NBF instead of the 40% formaldehyde. Fortunately, I hadn't ruined any tissue, and all I had to do was make up new alcoholic formalin using the correct chemicals. However, that episode did teach me what can happen when I don't check each label before I use a chemical. And, I did get to see what happens when buffering salts and alcohol are mixed together. And this mistake then allowed me, many years later (now), to help Jennifer figure out her cloudiness problem. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 _____ From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Wednesday, May 21, 2008 9:29 AM To: Jennifer Johnson; lpwenk@sbcglobal.net; histonet@lists.utsouthwestern.edu; Elaine Smith Subject: RE: [Histonet] Formaldehyde + Acetic Acid Peggy showed why she had been awarded "Histotech of the Year" with her very clever and insightful answer. The salts dissolved in the NBF will precipitate with the alcohol. That is why the tissue processors have to be cleaned with hot hater (to eliminate the salts by dissolving them), or why some people (like me) used to use 10% formalin NOT buffered in the first 2 stations of the VIP (to avoid the salt precipitates). Ren? J. Jennifer Johnson wrote: > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _____ Give to a good cause with every e-mail. Join the i?m Initiative from Microsoft. From BMolinari <@t> heart.thi.tmc.edu Thu May 22 05:35:35 2008 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Thu May 22 05:35:33 2008 Subject: [Histonet] Richard Allan Hematoxylin problems In-Reply-To: <20080521201022.D9119D2AEB@swiftsure.cnchost.com> References: <80ab7bc60805201220h44cb0e54y8223f5231a7fc495@mail.gmail.com><007701c8bab4$2e2d27d0$6501a8c0@DHXTS541> <20080521201022.D9119D2AEB@swiftsure.cnchost.com> Message-ID: Phew, I thought it was me. I use Hematoxylin 1 (7221) and have the same issue. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,Texas 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Wednesday, May 21, 2008 3:10 PM To: Angela Bitting; Gayle Callis; Patty Dunlop; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Richard Allan Hematoxylin problems Yes, I am experiencing the same problem. I haven't changed my protocol, since it's been in place now for the past 6 years. About three weeks ago, my pathologist started complaining that the nuclei is too pale. I use their 7211 and I asked for a sample of their Hematoxylin 1. I'll see if it produces a darker stain. Paula ---- Angela Bitting wrote: > > We have always changed our hematoxylin every Sunday night, and just filtered on the days in between. We've had no change in volumes. Now, all of a sudden, by Tuesday my hematoxylin staining is so pale that the docs are screaming. This has been going on for about two weeks now. We use Richard Allan Modified Harris Hematoxylin. Anyone else seen a change with that brand? I know there is supposed to be a "shortage" of hematoxylin right now. > > Angela Bitting, HT(ASCP) > Technical Specialist, Histology > Geisinger Medical Center > 100 N Academy Ave. MC 23-00 > Danville, PA 17822 > phone 570-214-9634 > fax 570-271-5916 > > No trees were hurt in the sending of this email > However many electrons were severly inconvienienced! > > > >>> "Gayle Callis" 5/20/2008 4:00 PM >>> > We use xylene to remove coverslips and do this work inside a hood. After > coverslip is off and mounting media is gone with an extra xylene rinse, we > go back to Clearite 3 to rinse off xylene and recoverslip. Anything to try > an minimize exposure to xylene is done. Unfortunately, we found the > substitute just didn't work for this job. > > Gayle M. Callis > HTL/HT/MT(ASCP) > Bozeman MT > > > ----- Original Message ----- > From: "Patty Dunlop" > To: > Sent: Tuesday, May 20, 2008 1:20 PM > Subject: [Histonet] xylene substitute recoverslipping > > > > Hello Histonetters, > > > > I use Clear-Rite 3, a xylene substitute, and am having problems when I > > need > > to recoverslip. Currently, I just put the slide that needs recoverslipped > > into a coplin jar of Clear-rite 3 at room temperature. The coverslip > > really > > doesn't like to come off (takes a week) and it tends to be a sticky, messy > > process. Does anyone have a method for removing coverslips with a xylene > > substitute? Should I use heat? > > > > Thanks, > > Patty > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu May 22 07:54:56 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 22 07:55:02 2008 Subject: [Histonet] Formaldehyde + Acetic Acid In-Reply-To: <000301c8bbee$0b0ea9a0$35ee2d4b@HPPav2> Message-ID: <226408.20148.qm@web65705.mail.ac4.yahoo.com> And that is what is called EXPERIENCE = the intelligent use of mistakes made along our life! Ren? J. Lee & Peggy Wenk wrote: Thanks for the nice words, but unfortunately, my answer was based on my own real life error. Some years ago, I was making alcoholic formalin for the tissue processor. I had already mixed the alcohol and water, then poured in the formaldehyde. And watched it turn cloudy milky white, to my utter amazement. My thoughts? - it's never done that before - I wonder if it will go away if I wait a couple of minutes (no) - I wonder if it will go away if I swirl the container and mix the solutions better (no) - I wonder what's wrong with the water today (no idea why I thought the water was the problem) Finally, I thought about looking at the labels on the solutions, and found that I had inadvertantly grabbed the 10% NBF instead of the 40% formaldehyde. Fortunately, I hadn't ruined any tissue, and all I had to do was make up new alcoholic formalin using the correct chemicals. However, that episode did teach me what can happen when I don't check each label before I use a chemical. And, I did get to see what happens when buffering salts and alcohol are mixed together. And this mistake then allowed me, many years later (now), to help Jennifer figure out her cloudiness problem. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 --------------------------------- From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Wednesday, May 21, 2008 9:29 AM To: Jennifer Johnson; lpwenk@sbcglobal.net; histonet@lists.utsouthwestern.edu; Elaine Smith Subject: RE: [Histonet] Formaldehyde + Acetic Acid Peggy showed why she had been awarded "Histotech of the Year" with her very clever and insightful answer. The salts dissolved in the NBF will precipitate with the alcohol. That is why the tissue processors have to be cleaned with hot hater (to eliminate the salts by dissolving them), or why some people (like me) used to use 10% formalin NOT buffered in the first 2 stations of the VIP (to avoid the salt precipitates). Ren? J. Jennifer Johnson wrote: .hmmessage P { PADDING-RIGHT: 0px; PADDING-LEFT: 0px; PADDING-BOTTOM: 0px; MARGIN: 0px; PADDING-TOP: 0px } BODY.hmmessage { FONT-SIZE: 10pt; FONT-FAMILY: Tahoma } > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > --------------------------------- Give to a good cause with every e-mail. Join the i?m Initiative from Microsoft. From kdboydhisto <@t> yahoo.com Thu May 22 08:51:19 2008 From: kdboydhisto <@t> yahoo.com (KELLY BOYD) Date: Thu May 22 08:51:22 2008 Subject: [Histonet] Hematoxylin shortage Message-ID: <303463.82470.qm@web58611.mail.re3.yahoo.com> Can someone please tell me what is up with all the talk about a hematoxylin shortage? There is even a vendor telling their customers they need to stock up. Is this fact or rumor? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 Tele (252)-830-6866 Cell (252)-943-9527 Fax (252)-830-0032 From rjbuesa <@t> yahoo.com Thu May 22 09:00:50 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 22 09:00:54 2008 Subject: [Histonet] Hematoxylin shortage In-Reply-To: <303463.82470.qm@web58611.mail.re3.yahoo.com> Message-ID: <160915.12883.qm@web65702.mail.ac4.yahoo.com> Never pay attention to a vendor urging to stock up. It just may be a gimmick to increase sales/commissions. There is nothing concrete so far about that rumor. Ren? J. KELLY BOYD wrote: Can someone please tell me what is up with all the talk about a hematoxylin shortage? There is even a vendor telling their customers they need to stock up. Is this fact or rumor? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 Tele (252)-830-6866 Cell (252)-943-9527 Fax (252)-830-0032 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jessica.Vacca <@t> HCAhealthcare.com Thu May 22 09:09:58 2008 From: Jessica.Vacca <@t> HCAhealthcare.com (Vacca Jessica) Date: Thu May 22 09:10:04 2008 Subject: [Histonet] Hematoxylin shortage In-Reply-To: <160915.12883.qm@web65702.mail.ac4.yahoo.com> References: <303463.82470.qm@web58611.mail.re3.yahoo.com> <160915.12883.qm@web65702.mail.ac4.yahoo.com> Message-ID: <41E16A15CE78374EA45B57E0F94339B803CDC47B@ORLEV01.hca.corpad.net> I have received an email from my Supply chain that states there is a hemo shortage due to the hurricanes last year. Thermo is offering a synthetic substitute. We order Hemo (Richard Allen) from Cardinal. I spoke with my sales rep, and it was confirmed, but he did state not to stock up because it would cause an even more chaotic situation of hoarding and not be able to keep up with the demand. Let's see what the future brings?? Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr. Brandon Fl 33511 (813) 571-5193 or (813) 681-5551 ext 2454 Jessica.Vacca@hcahealthcare.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, May 22, 2008 10:01 AM To: KELLY BOYD; histonet Subject: Re: [Histonet] Hematoxylin shortage Never pay attention to a vendor urging to stock up. It just may be a gimmick to increase sales/commissions. There is nothing concrete so far about that rumor. Ren? J. KELLY BOYD wrote: Can someone please tell me what is up with all the talk about a hematoxylin shortage? There is even a vendor telling their customers they need to stock up. Is this fact or rumor? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 Tele (252)-830-6866 Cell (252)-943-9527 Fax (252)-830-0032 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Thu May 22 09:10:01 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Thu May 22 09:10:11 2008 Subject: [Histonet] Factor 13a Message-ID: Good morning everyone, My latest lot of Factor 13a, from a reputable vendor, has "pooped out" It tests out fine at their facility but is much weaker on my Benchmark, XT and NexES, with DAB detection, than the previous lot and is basically negative with Alk. Phos. Red detection. So my question is, which F13a are people using reliably on Ventana instruments? Thanks for the help, Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From mribeiro <@t> brinegroup.com Thu May 22 09:24:22 2008 From: mribeiro <@t> brinegroup.com (Melissa Ribeiro) Date: Thu May 22 09:24:28 2008 Subject: [Histonet] Histology Supervisor - IHC Message-ID: <0D88BB18E51FE3449EB2D2F505936A7A4ADB9C@brinedc.brine.local> Our firm has been retained to identify a candidate with a strong IHC background for a Histology Supervisor position in a Massachusetts Teaching Hospital. Any qualified and interested candidates, please feel free to send resumes to mribeiro@brinegroup.com or call (781) 272-3400 Melissa Ribeiro Healthcare Division Brine Group Staffing Solutions 20 Mall Road, Suite 225 Burlington, MA 01803 mribeiro@brinegroup.com Ph. (781) 272-3400 ext. 228 Fax (781) 494-3401 Visit our newly redesigned web site at www.brinegroup.com , the intelligent choice for staffing solutions. This electronic message contains information from Brine Group Staffing Solutions that may be privileged and confidential. The information is intended for the use of the addressee(s) only. If you are not an addressee, or received this email in error, note that any disclosure, copying, distribution, or use of the contents of this message is prohibited. If you have received this E-mail in error, please contact the sender immediately. From Lynn.Burton <@t> Illinois.gov Thu May 22 09:23:09 2008 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Thu May 22 09:25:47 2008 Subject: [Histonet] Hematoxylin shortage References: <303463.82470.qm@web58611.mail.re3.yahoo.com> Message-ID: I was recently at a meeting for ISH, and this has been reported on this venue previously, and all vendors and some speakers confirmed this to be true. They do say that the shortage should clear up in a few months. They also said that stock piling was not a good idea. Better to stretch your supply as much as possible. Lynn Burton Animal Disease Lab Galesburg,Il ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of KELLY BOYD Sent: Thu 5/22/2008 8:51 AM To: histonet Subject: [Histonet] Hematoxylin shortage Can someone please tell me what is up with all the talk about a hematoxylin shortage? There is even a vendor telling their customers they need to stock up. Is this fact or rumor? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 Tele (252)-830-6866 Cell (252)-943-9527 Fax (252)-830-0032 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From fudo <@t> ufl.edu Thu May 22 09:36:46 2008 From: fudo <@t> ufl.edu (FU,DONGTAO) Date: Thu May 22 09:36:50 2008 Subject: [Histonet] PH of Toluidine blue and aldehyde fuscin staining Message-ID: <1056368044.242941211467006107.JavaMail.osg@osgjas01.cns.ufl.edu> Hi, all we will do some special staining of T blue and aldehyde fuscin. We will make some solutions by ourself. I notice some buffers need to measure PH value. But they are dyes, so you can not use dip paper. How do you measure them? Thank you, Ann From BNuern <@t> coj.net Thu May 22 09:36:58 2008 From: BNuern <@t> coj.net (Nuernberger, Barb) Date: Thu May 22 09:37:51 2008 Subject: [Histonet] osmium Message-ID: <66529604C8644845BB24FF6F6235664101FCC33C@EVS1.coj.net> I'm asking this on behalf of my histologist who has been asked by a pathologist to modify a protocol that she has from the AFIP manual. When doing an osmium fixation on formalin fixed tissue, is the 8 hour fixing time a minimum or can it sit overnight before continuing? Thank you for any help. Barb Nuernberger Chief Toxicologist Medical Examiner's Office 2100 Jefferson St. Jacksonville,FL 32206 (904)630-5470 BNuern@coj.net From sbreeden <@t> nmda.nmsu.edu Thu May 22 09:45:56 2008 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu May 22 09:46:07 2008 Subject: [Histonet] Manual BVD Procedure Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E631D@nmdamailsvr.nmda.ad.nmsu.edu> I have a visiting veterinary pathologist from Mexico this week; he is in need of a manual procedure for BVD. I run the Ventana system and am no help to him - could someone kindly share their manual BVD protocol with me? And perhaps a source for the antibody for same? My thanks! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From crochieresteve <@t> aol.com Thu May 22 09:49:01 2008 From: crochieresteve <@t> aol.com (crochieresteve@aol.com) Date: Thu May 22 09:49:12 2008 Subject: [Histonet] HIER to repair dried specimens? Message-ID: <8CA8A321B9E08F5-6B4-1295@webmail-nf15.sim.aol.com> I have accidentally stumbled on an unexplainable "by product" of HIER in citrate buffer, pH6.0. We had some specimens that had unfortunately air dried prior to fixation and processing and therefore looked terrible. The H&E was practically unreadable due to drying artifact. In an effort to see something worthwhile, IHC stains were ordered. The hematoxylin counterstain, following retrieval showed much better nuclear detail than the original H&E. Several additional slides were cut and stained with H&E following HIER. They were much better. This has been repeated on a few other specimens with similar results. Any ideas as to how or why this is working? thanks sc From oshel1pe <@t> cmich.edu Thu May 22 10:03:38 2008 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Thu May 22 10:03:49 2008 Subject: [Histonet] osmium In-Reply-To: <66529604C8644845BB24FF6F6235664101FCC33C@EVS1.coj.net> References: <66529604C8644845BB24FF6F6235664101FCC33C@EVS1.coj.net> Message-ID: Discounting regulations, generally osmium fixation can be done overnight at 4 deg C (refrigerator temperature). Mind, OsO4 is slow penetrating, and overnight might be required simply because of the size of the tissue blocks. Room temp ... mayyyyyybbbeee given the tissue requirements. But OsO4 makes tissues brittle and crunchy, so that usually isn't a good idea. Phil >I'm asking this on behalf of my histologist who has been asked by a >pathologist to modify a protocol that she has from the AFIP manual. >When doing an osmium fixation on formalin fixed tissue, is the 8 hour >fixing time a minimum or can it sit overnight before continuing? > >Thank you for any help. >Barb Nuernberger >Chief Toxicologist > > >Medical Examiner's Office >2100 Jefferson St. >Jacksonville,FL 32206 >(904)630-5470 >BNuern@coj.net -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576 From Robinsoc <@t> mercyhealth.com Thu May 22 10:11:54 2008 From: Robinsoc <@t> mercyhealth.com (Cynthia Robinson) Date: Thu May 22 10:12:18 2008 Subject: [Histonet] Hematoxylin shortage In-Reply-To: <160915.12883.qm@web65702.mail.ac4.yahoo.com> References: <303463.82470.qm@web58611.mail.re3.yahoo.com> <160915.12883.qm@web65702.mail.ac4.yahoo.com> Message-ID: <483546EA.59BC.00AF.0@mercyhealth.com> I use Thermo Shandon as a supplier for my hematoxylin and just got an order placed on back order until the end of 2008. Cindi Robinson HT(ASCP) Mercy Medical Center-Sioux City Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes, SD 57049 >>> Rene J Buesa 5/22/2008 9:00 AM >>> Never pay attention to a vendor urging to stock up. It just may be a gimmick to increase sales/commissions. There is nothing concrete so far about that rumor. Ren? J. KELLY BOYD wrote: Can someone please tell me what is up with all the talk about a hematoxylin shortage? There is even a vendor telling their customers they need to stock up. Is this fact or rumor? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 Tele (252)-830-6866 Cell (252)-943-9527 Fax (252)-830-0032 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From o.m.gallagher <@t> sheffield.ac.uk Thu May 22 10:19:51 2008 From: o.m.gallagher <@t> sheffield.ac.uk (Orla Gallagher) Date: Thu May 22 10:19:56 2008 Subject: [Histonet] Microtome blades jammed in dispensing box Message-ID: <48359D26.20553.15909D5@localhost> Dear Histonetters, We've been having a problem over the past year or 2 with our microtome blades becoming jammed in the dispensing box after only a few have been used and wondered if this has happened to anyone else? Our refurbished building has a cooling system without humidity; would this cause the blades to become jammed? It's happened with Feather S35 & N35 blades and Accu Edge blades from different suppliers and in more than one lab in the building. Any advice on how to get the blades out would be much appreciated! Many thanks, Orla ****************************** Ms. Orla Gallagher Academic Unit of Bone Biology D Floor (DU20) Medical School Henry Wellcome Laboratories for Medical Research University of Sheffield Beech Hill Road Sheffield S10 2RX www.sheffield.ac.uk E-mail:o.m.gallagher@sheffield.ac.uk Tel: 0114 271 3783(lab) 0114 271 3337(office) Fax: 0114 271 1711 From rjr6 <@t> psu.edu Thu May 22 10:33:14 2008 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Thu May 22 10:33:20 2008 Subject: [Histonet] Microtome blades jammed in dispensing box In-Reply-To: <48359D26.20553.15909D5@localhost> References: <48359D26.20553.15909D5@localhost> Message-ID: I have had this happen several times. I found that if the dispenser button is pulled back so the next blade is ready to dispense I don't have it happen. When it does happen I have taken the dispenser apart carefully and separate the blades into a box lid. Roberta Horner HT/HTL Penn State University Animal Diagnostic Lab From bhewlett <@t> cogeco.ca Thu May 22 10:38:28 2008 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Thu May 22 10:38:43 2008 Subject: [Histonet] HIER to repair dried specimens? References: <8CA8A321B9E08F5-6B4-1295@webmail-nf15.sim.aol.com> Message-ID: <000501c8bc21$e4296580$6700a8c0@mainbox> It's called re-hydration! Similar techniques are used for partial restoration of mummified tissue for histological examination. Bryan \ ----- Original Message ----- From: To: Sent: Thursday, May 22, 2008 10:49 AM Subject: [Histonet] HIER to repair dried specimens? >I have accidentally stumbled on an unexplainable "by product" of HIER in >citrate buffer, pH6.0. > We had some specimens that had unfortunately air dried prior to fixation > and processing and therefore looked terrible. The H&E was practically > unreadable due to drying artifact. In an effort to see something > worthwhile, IHC stains were ordered. The hematoxylin counterstain, > following retrieval showed much better nuclear detail than the original > H&E. Several additional slides were cut and stained with H&E following > HIER. They were much better. This has been repeated on a few other > specimens with similar resu > lts. Any ideas as to how or why this is working? > thanks > sc > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From RSalcedo <@t> tmhs.org Thu May 22 10:39:53 2008 From: RSalcedo <@t> tmhs.org (Salcedo, Rudy) Date: Thu May 22 10:40:13 2008 Subject: [Histonet] Leica Peloris Message-ID: <0DE2321B1716814E9DF3B6753171E72201133632@EXCH9.tmh.tmhs> Histonet, Any labs out there using the Leica Peloris Processor, if so please send me some feed back on you experience with the Peloris. Thanks in advance. Rudy Salcedo Houston, Texas NOTICE: Our e-mail address at Methodist has changed to tmhs.org Methodist. Leading Medicine. Ranked No. 10 on FORTUNE magazine's list of the "100 Best Companies to Work For" in 2008 Named by U.S. News & World Report as one of "America's Best Hospitals" Designated as a Magnet hospital for excellence in nursing ***CONFIDENTIALITY NOTICE*** This e-mail is the property of The Methodist Hospital and/or its relevant affiliates and may contain confidential and privileged material for the sole use of the intended recipient(s). Any review, use, distribution or disclosure by others is strictly prohibited. If you are not the intended recipient (or authorized to receive for the recipient), please contact the sender and delete all copies of the message. Thank you. From mabosso <@t> unipathllc.com Thu May 22 10:43:18 2008 From: mabosso <@t> unipathllc.com (Mary Abosso) Date: Thu May 22 10:43:35 2008 Subject: [Histonet] Richard Allan Hematoxylin problems References: <80ab7bc60805201220h44cb0e54y8223f5231a7fc495@mail.gmail.com><007701c8bab4$2e2d27d0$6501a8c0@DHXTS541><48343D2A.2B7F.00C9.0@geisinger.edu> <48344E43.CC0E.007B.0@vetmed.ufl.edu> Message-ID: <43A451981FF6634795BE83B1B5494D6313647D@exchange.unipathllc.corp> I was the QA Histotechnologist at Richard-Allan and the best way to handle any product issues is to call in to Tech-Rite technical support who can help figure out what the issue is. Additonally, they will file a complaint with the QA department who will thouroughly investigate the retain sample from that lot, all of the chemicals used in the batch. Richard-Allan (Thermo-Fisher Scientific) is committed to the highest standard of quality and needs the customer feed back to continuously improve. Mary Abosso Unipath LLC. Denver, CO ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Shawn Leslie Sent: Wed 5/21/2008 2:30 PM To: Angela Bitting Cc: HistoNet Subject: Re: [Histonet] Richard Allan Hematoxylin problems We also use the Richard Allen 7211 but we haven't had any complaints. Maybe it's just a bad batch. Contact the vendor I am sure they will fix it.... Shawn Leslie HT (ASCP) Scientific Research Manager Anatomic Pathology University of Florida School of Veterinary Medicine 352-392-2235 ext 4555 >>> "Angela Bitting" 5/21/2008 3:18 PM >>> We have always changed our hematoxylin every Sunday night, and just filtered on the days in between. We've had no change in volumes. Now, all of a sudden, by Tuesday my hematoxylin staining is so pale that the docs are screaming. This has been going on for about two weeks now. We use Richard Allan Modified Harris Hematoxylin. Anyone else seen a change with that brand? I know there is supposed to be a "shortage" of hematoxylin right now. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> "Gayle Callis" 5/20/2008 4:00 PM >>> We use xylene to remove coverslips and do this work inside a hood. After coverslip is off and mounting media is gone with an extra xylene rinse, we go back to Clearite 3 to rinse off xylene and recoverslip. Anything to try an minimize exposure to xylene is done. Unfortunately, we found the substitute just didn't work for this job. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT ----- Original Message ----- From: "Patty Dunlop" To: Sent: Tuesday, May 20, 2008 1:20 PM Subject: [Histonet] xylene substitute recoverslipping > Hello Histonetters, > > I use Clear-Rite 3, a xylene substitute, and am having problems when I > need > to recoverslip. Currently, I just put the slide that needs recoverslipped > into a coplin jar of Clear-rite 3 at room temperature. The coverslip > really > doesn't like to come off (takes a week) and it tends to be a sticky, messy > process. Does anyone have a method for removing coverslips with a xylene > substitute? Should I use heat? > > Thanks, > Patty > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgalnow <@t> aol.com Thu May 22 10:47:39 2008 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Thu May 22 10:47:44 2008 Subject: [Histonet] now hiring Message-ID: <8CA8A3A4C2B73E8-D34-22E4@FWM-D26.sysops.aol.com> Hey--- I know that you all have seen my posting for our salary ranges here and it has caused much disagreement, sorry about that!? However, I am now looking for a histotechnologist (Florida licensed as a technologist, or eligible), HT(ASCP), with QIHC preferred.? Extensive knowledge of IHC is needed, prefer molecular testing background.? MP certification would be great....... Excellent pay and bennies.......in sunny Tampa, Florida And the best part is....you get to work with me (ha ha). Roxanne From contact <@t> excaliburpathology.com Thu May 22 10:48:51 2008 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Thu May 22 10:49:00 2008 Subject: [Histonet] Osmium Message-ID: <151803.29445.qm@web50108.mail.re2.yahoo.com> Are the specimens to be used for electron microscopy or fat stains? How big are they? Tell her to be very careful when using osmium. The fumes will fix your corneas. ?Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 631 N. Broadway Ave. Moore, OK 73160 405-570-6679 cell 405-759-3953 lab contact@excaliburpathology.com www.excaliburpathology.com From crochieresteve <@t> aol.com Thu May 22 10:51:48 2008 From: crochieresteve <@t> aol.com (crochieresteve@aol.com) Date: Thu May 22 10:51:55 2008 Subject: [Histonet] HIER to repair dried specimens? In-Reply-To: <000501c8bc21$e4296580$6700a8c0@mainbox> References: <8CA8A321B9E08F5-6B4-1295@webmail-nf15.sim.aol.com> <000501c8bc21$e4296580$6700a8c0@mainbox> Message-ID: <8CA8A3AE0814351-87C-14B7@webmail-nb18.sysops.aol.com> I'm familiar with rehydrating mummy tissue from my years at AFIP. We used formol-glycol for several days prior to processing. I didn't think boiling slides in buffer would "repair" "cooked" tissue, but it seems to be so. Thanks s -----Original Message----- From: Bryan Hewlett To: histonet@pathology.swmed.edu; crochieresteve@aol.com Sent: Thu, 22 May 2008 11:38 am Subject: Re: [Histonet] HIER to repair dried specimens? It's called re-hydration!? Similar techniques are used for partial restoration of mummified tissue for histological examination.? ? Bryan? \? ? ----- Original Message ----- From: ? To: ? Sent: Thursday, May 22, 2008 10:49 AM? Subject: [Histonet] HIER to repair dried specimens?? ? >I have accidentally stumbled on an unexplainable "by product" of HIER in >citrate buffer, pH6.0.? > We had some specimens that had unfortunately air dried prior to fixation > and processing and therefore looked terrible. The H&E was practically > unreadable due to drying artifact. In an effort to see something > worthwhile, IHC stains were ordered. The hematoxylin counterstain, > following retrieval showed much better nuclear detail than the original > H&E. Several additional slides were cut and stained with H&E following > HIER. They were much better. This has been repeated on a few other > specimens with similar resu? > lts. Any ideas as to how or why this is working?? > thanks? > sc? > _______________________________________________? > Histonet mailing list? > Histonet@lists.utsouthwestern.edu? > http://lists.utsouthwestern.edu/mailman/listinfo/histonet? > ? From contact <@t> excaliburpathology.com Thu May 22 10:53:36 2008 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Thu May 22 10:53:47 2008 Subject: [Histonet] Microtome blades jammed in dispenser box Message-ID: <516735.64474.qm@web50109.mail.re2.yahoo.com> I am having the same problem. The tab that grabs the hole in the blades to push them forward tends to wear more than in the past and does not grab after a few have been dispensed. I have taken a very fine pair of forceps and helped the blade along and when that no loner works because the blade does not move at all I took the box apart. ?Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 631 N. Broadway Ave. Moore, OK 73160 405-570-6679 cell 405-759-3953 lab contact@excaliburpathology.com www.excaliburpathology.com From mpence <@t> grhs.net Thu May 22 11:18:45 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Thu May 22 11:18:51 2008 Subject: [Histonet] Leica Peloris In-Reply-To: <0DE2321B1716814E9DF3B6753171E72201133632@EXCH9.tmh.tmhs> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3849@IS-E2K3.grhs.net> I just purchased a Peloris about 3 months ago. Love it. I can process all thru the day and it has made a huge impact on turn around times. We cut fewer blocks thru out the day and I think it processes fatty specimens better. I have a few small issues, but I have been very happy with the processor. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Salcedo, Rudy Sent: Thursday, May 22, 2008 10:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Peloris Histonet, Any labs out there using the Leica Peloris Processor, if so please send me some feed back on you experience with the Peloris. Thanks in advance. Rudy Salcedo Houston, Texas NOTICE: Our e-mail address at Methodist has changed to tmhs.org Methodist. Leading Medicine. Ranked No. 10 on FORTUNE magazine's list of the "100 Best Companies to Work For" in 2008 Named by U.S. News & World Report as one of "America's Best Hospitals" Designated as a Magnet hospital for excellence in nursing ***CONFIDENTIALITY NOTICE*** This e-mail is the property of The Methodist Hospital and/or its relevant affiliates and may contain confidential and privileged material for the sole use of the intended recipient(s). Any review, use, distribution or disclosure by others is strictly prohibited. If you are not the intended recipient (or authorized to receive for the recipient), please contact the sender and delete all copies of the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terri.Brown <@t> Northside.com Thu May 22 11:21:46 2008 From: Terri.Brown <@t> Northside.com (Terri Brown) Date: Thu May 22 11:22:12 2008 Subject: [Histonet] Hematoxylin shortage In-Reply-To: <483546EA.59BC.00AF.0@mercyhealth.com> References: <303463.82470.qm@web58611.mail.re3.yahoo.com> <160915.12883.qm@web65702.mail.ac4.yahoo.com> <483546EA.59BC.00AF.0@mercyhealth.com> Message-ID: <8CEB6DA1A3F35743800669D4CFE21F7D03F8FFE0@NSMXMS04.northside.local> I heard that this was going to happen and ordered the powder so we could make our own. It is on backorder too. I ordered it back in April. Terri Brown, HT (ASCP) Northside Hospital-Atlanta, Ga. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Robinson Sent: Thursday, May 22, 2008 11:12 AM To: histonet; KELLY BOYD; Rene J Buesa Subject: Re: [Histonet] Hematoxylin shortage I use Thermo Shandon as a supplier for my hematoxylin and just got an order placed on back order until the end of 2008. Cindi Robinson HT(ASCP) Mercy Medical Center-Sioux City Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes, SD 57049 >>> Rene J Buesa 5/22/2008 9:00 AM >>> Never pay attention to a vendor urging to stock up. It just may be a gimmick to increase sales/commissions. There is nothing concrete so far about that rumor. Ren? J. KELLY BOYD wrote: Can someone please tell me what is up with all the talk about a hematoxylin shortage? There is even a vendor telling their customers they need to stock up. Is this fact or rumor? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 Tele (252)-830-6866 Cell (252)-943-9527 Fax (252)-830-0032 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. From mcauliff <@t> umdnj.edu Thu May 22 12:05:23 2008 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu May 22 12:05:09 2008 Subject: [Histonet] osmium In-Reply-To: <66529604C8644845BB24FF6F6235664101FCC33C@EVS1.coj.net> References: <66529604C8644845BB24FF6F6235664101FCC33C@EVS1.coj.net> Message-ID: <4835A7D3.7030306@umdnj.edu> You can fix the tissue overnight either cold or at room temp. but osmium will only penetrate about 1mm no matter how long you fix. And it will make the tissue VERY brittle as Phil mentioned. Seriously consider cutting frozen sections and osmicating them, no penetration or brittle sections problems! You MUST open the bottles/vials etc under a hood, osmium will fix your corneas! Geoff Nuernberger, Barb wrote: > I'm asking this on behalf of my histologist who has been asked by a > pathologist to modify a protocol that she has from the AFIP manual. > When doing an osmium fixation on formalin fixed tissue, is the 8 hour > fixing time a minimum or can it sit overnight before continuing? > > Thank you for any help. > Barb Nuernberger > Chief Toxicologist > > > Medical Examiner's Office > 2100 Jefferson St. > Jacksonville,FL 32206 > (904)630-5470 > BNuern@coj.net > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From PIXLEYSK <@t> UCMAIL.UC.EDU Thu May 22 12:16:37 2008 From: PIXLEYSK <@t> UCMAIL.UC.EDU (Pixley, Sarah (pixleysk)) Date: Thu May 22 12:16:44 2008 Subject: [Histonet] Staining cultured cells with Giemsa In-Reply-To: <200805221413.JEC82574@mprelay2.uc.edu> Message-ID: <771F0CF37F5F7E44884D8022D2A3EAB40316632A@ucmail6.ad.uc.edu> Here is my staining procedure for monolayer cells in culture. As a previous person mentioned, use your common sense to modify. But I worked with it enough to know that the following steps worked for me. Giemsa Stain: Use Giemsa stain from Sigma: ?Rinse live cells 1x with PBS 1X (warranted to get rid of phenol red and any serum) Fix in cold 100% Methanol, 5-7 mins Air Dry Dilute Giemsa 1/20 with ddWater. ? Maybe it would be wise next time to filter, Whatman#1 Stain 15-60 mins. For cultured cell monolayers, 15 was enough. Rinse with ddWater. ~5-6 times. Air dry Can mount with aqueous mounting medium. Sarah Pixley From thoward <@t> unm.edu Thu May 22 12:49:13 2008 From: thoward <@t> unm.edu (Tamara A Howard) Date: Thu May 22 12:49:17 2008 Subject: [Histonet] RE: Microtome blades jammed in dispensing box Message-ID: I've had boxes of blades that did this - I use a needle probe to poke the top 2 blades apart (poking through the little window where the slider goes). It takes a few tries sometimes, but once you can separate the top blade it slides out in a jiffy. Tamara *************************** Tamara Howard Cell Biology & Physiology UNM-HSC Albuquerque, NM *************************** From sbreeden <@t> nmda.nmsu.edu Thu May 22 12:57:29 2008 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu May 22 12:57:36 2008 Subject: [Histonet] Blades & Hematoxylin Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E6323@nmdamailsvr.nmda.ad.nmsu.edu> 1. Blades: I use the Sakura/Feathers and I just give the container a good "whack" (on the bottom) on the counter before I dispense the first blade. They have no choice but to be compliant after that. even think about our humidity level in NM at about 10%... 2. Hematoxylin: I have been reluctant because of my "position" as president of the New Mexico Society for Histology to endorse products in this venue, but the hematoxylin issue makes it necessary for me to break my rule. I recently changed to the Surgipath "SelecTech" system and I have to report rave reviews by my pathologists. The hematoxylin is good for TWENTY-FIVE HUNDRED slides - the last slides being at least as good as the first - and I've had no difficulty in getting a supply. Shelf life is 2 years. Stain quality is superb. Cost is extremely reasonable for the quality. My autostainer results had been dramatically variable until I made the change to SelecTech and although I had tried it as an economic move, it has proved to be that and more. The SelecTech system gives me beautiful results. Bosses are happy as clams with the stain now! 3. How happy IS a clam??? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From sbreeden <@t> nmda.nmsu.edu Thu May 22 12:58:54 2008 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu May 22 12:59:00 2008 Subject: [Histonet] Blades & Hematoxylin, Part 2 Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E6324@nmdamailsvr.nmda.ad.nmsu.edu> I hit some key before I was finished proofing my post so the fact that it may not make sense is not my fault! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From laurie.colbert <@t> huntingtonhospital.com Thu May 22 13:16:05 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Thu May 22 13:16:11 2008 Subject: [Histonet] Leica Peloris Message-ID: <57BE698966D5C54EAE8612E8941D768303019248@EXCHANGE3.huntingtonhospital.com> Do you use xylene on the Peloris? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Thursday, May 22, 2008 9:19 AM To: Salcedo, Rudy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica Peloris I just purchased a Peloris about 3 months ago. Love it. I can process all thru the day and it has made a huge impact on turn around times. We cut fewer blocks thru out the day and I think it processes fatty specimens better. I have a few small issues, but I have been very happy with the processor. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Salcedo, Rudy Sent: Thursday, May 22, 2008 10:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Peloris Histonet, Any labs out there using the Leica Peloris Processor, if so please send me some feed back on you experience with the Peloris. Thanks in advance. Rudy Salcedo Houston, Texas NOTICE: Our e-mail address at Methodist has changed to tmhs.org Methodist. Leading Medicine. 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Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kdboydhisto <@t> yahoo.com Thu May 22 13:32:42 2008 From: kdboydhisto <@t> yahoo.com (KELLY BOYD) Date: Thu May 22 13:32:46 2008 Subject: [Histonet] processor schedule Message-ID: <764609.46678.qm@web58610.mail.re3.yahoo.com> What is a good time schedule for processing small dermatology biopsies?(no excisions) Not for a rush run, just a regular overnight run. I would like to know the best alcohol percentages and whether or not to use pressure or vacuum. We do use a lot of the blue sponges to flatten out specimens that tend to curl up. We put them on an old VIP or a VIP 5 processor. All of these biopsies have been fixed at least 24 hours before we receive them. After they are grossed in, they are placed in water instead of formalin to cut back on fumes. Then they are put on the processor with formalin as the first two stations. Can this harm tissue? Thanks! Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 Tele (252)-830-6866 Cell (252)-943-9527 Fax (252)-830-0032 From anitaibsc <@t> aol.com Thu May 22 13:33:10 2008 From: anitaibsc <@t> aol.com (anitaibsc@aol.com) Date: Thu May 22 13:33:25 2008 Subject: [Histonet] Hematoxylin Counter Stain - Available now Message-ID: <8CA8A516B92E659-147C-20FB@webmail-nd19.sysops.aol.com> Hello Netters, You can order Hematoxylin of excellent quality for IHC and H&E staining,?equivalent to Biomeda product, from ImmunoBioScience Corp. available for immediate delivery. Cat# AR-6521 -01 (15ml) for $50.00; -02 (100ml) for $90.00; -03 (250ml) for $150.00; -05 (1000ml) for $250.00. Please email anitaIBSC@aol.com Thank you. Anita Hingorani 650-343-IBSC From algranth <@t> u.arizona.edu Thu May 22 14:01:52 2008 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Thu May 22 14:11:30 2008 Subject: [Histonet] Blades & Hematoxylin Message-ID: <6.2.3.4.1.20080522115717.01f8f950@algranth.inbox.email.arizona.edu> Sally is right - Surgipath's new hematoxylin is great. I have just tried it out and changed over from the RAS Hematoxylin 7211. The stain is nice and clear - very crisp and it does last a long time. Love it! Andi ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From rjbuesa <@t> yahoo.com Thu May 22 14:38:49 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 22 14:38:59 2008 Subject: [Histonet] PH of Toluidine blue and aldehyde fuscin staining In-Reply-To: <1056368044.242941211467006107.JavaMail.osg@osgjas01.cns.ufl.edu> Message-ID: <119670.71119.qm@web65706.mail.ac4.yahoo.com> You have to measure the pH value of the solution once it is prepared. Being colored solutions it will be difficult to use a pH paper. Ideally you should use a pH-meter with a glass electrode. Ren? J. "FU,DONGTAO" wrote: Hi, all we will do some special staining of T blue and aldehyde fuscin. We will make some solutions by ourself. I notice some buffers need to measure PH value. But they are dyes, so you can not use dip paper. How do you measure them? Thank you, Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu May 22 14:46:40 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 22 14:46:43 2008 Subject: [Histonet] processor schedule In-Reply-To: <764609.46678.qm@web58610.mail.re3.yahoo.com> Message-ID: <415543.22577.qm@web65715.mail.ac4.yahoo.com> Kelly: You have limited the options because you are talking about "overnight". I also strongly recommend you to stop that practice of putting the biopsies in water after being fixed. That is no good procedure. If you have a problem with the fumes, place all the cassettes with formalin in a fumes hood. Pressure - vacuum in the tissue processor is to assure better exchange of reagents and it should be used if your tissue processor has that capability (as all VIPs do). You can start in 80% EthOL and if the biopsies have fat you should increase the xylene times. A 4 hours protocol will be more than enough. Try that the end of the processing time coincides with the staring time for embedding, which means that the specimens should be in formalin (first station) until the process starts. Ren? J. KELLY BOYD wrote: From rjbuesa <@t> yahoo.com Thu May 22 14:48:16 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 22 14:48:20 2008 Subject: [Histonet] Hematoxylin Counter Stain - Available now In-Reply-To: <8CA8A516B92E659-147C-20FB@webmail-nd19.sysops.aol.com> Message-ID: <47849.48910.qm@web65711.mail.ac4.yahoo.com> With those retail prices ($250/liter) I am willing to prepare some hematoxylin from some old powder I have and sell it! Ren? J. anitaibsc@aol.com wrote: Hello Netters, You can order Hematoxylin of excellent quality for IHC and H&E staining,?equivalent to Biomeda product, from ImmunoBioScience Corp. available for immediate delivery. Cat# AR-6521 -01 (15ml) for $50.00; -02 (100ml) for $90.00; -03 (250ml) for $150.00; -05 (1000ml) for $250.00. Please email anitaIBSC@aol.com Thank you. Anita Hingorani 650-343-IBSC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jtaylor <@t> meriter.com Thu May 22 15:05:00 2008 From: jtaylor <@t> meriter.com (Taylor, Jean) Date: Thu May 22 15:06:07 2008 Subject: [Histonet] p53 Message-ID: <328CBAE62F31C642B422970E879DFADC03A36954@pcwex01> Hi everyone, I am wondering what clone of p53 labs are using. I currently use Dako's DO-7 clone but am also testing NeoMarker's rabbit monoclonal SP5. I've gotten very different results comparing the two and would like to know what the majority of labs are using. Thanks! Jean Taylor HT(ASCP)QIHC Meriter Labs Madison, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, May 22, 2008 12:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 54, Issue 35 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Factor 13a (Sebree Linda A.) 2. Histology Supervisor - IHC (Melissa Ribeiro) 3. RE: Hematoxylin shortage (Burton, Lynn) 4. PH of Toluidine blue and aldehyde fuscin staining (FU,DONGTAO) 5. osmium (Nuernberger, Barb) 6. Manual BVD Procedure (Breeden, Sara) 7. HIER to repair dried specimens? (crochieresteve@aol.com) 8. Re: osmium (Philip Oshel) 9. Re: Hematoxylin shortage (Cynthia Robinson) 10. Microtome blades jammed in dispensing box (Orla Gallagher) 11. RE: Microtome blades jammed in dispensing box (Roberta Horner) 12. Re: HIER to repair dried specimens? (Bryan Hewlett) 13. Leica Peloris (Salcedo, Rudy) 14. RE: Richard Allan Hematoxylin problems (Mary Abosso) 15. now hiring (godsgalnow@aol.com) 16. Osmium (Paula Pierce) 17. Re: HIER to repair dried specimens? (crochieresteve@aol.com) 18. Microtome blades jammed in dispenser box (Paula Pierce) 19. RE: Leica Peloris (Mike Pence) 20. RE: Hematoxylin shortage (Terri Brown) ---------------------------------------------------------------------- Message: 1 Date: Thu, 22 May 2008 09:10:01 -0500 From: "Sebree Linda A." Subject: [Histonet] Factor 13a To: "Histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" Good morning everyone, My latest lot of Factor 13a, from a reputable vendor, has "pooped out" It tests out fine at their facility but is much weaker on my Benchmark, XT and NexES, with DAB detection, than the previous lot and is basically negative with Alk. Phos. Red detection. So my question is, which F13a are people using reliably on Ventana instruments? Thanks for the help, Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 ------------------------------ Message: 2 Date: Thu, 22 May 2008 10:24:22 -0400 From: "Melissa Ribeiro" Subject: [Histonet] Histology Supervisor - IHC To: Message-ID: <0D88BB18E51FE3449EB2D2F505936A7A4ADB9C@brinedc.brine.local> Content-Type: text/plain; charset="US-ASCII" Our firm has been retained to identify a candidate with a strong IHC background for a Histology Supervisor position in a Massachusetts Teaching Hospital. Any qualified and interested candidates, please feel free to send resumes to mribeiro@brinegroup.com or call (781) 272-3400 Melissa Ribeiro Healthcare Division Brine Group Staffing Solutions 20 Mall Road, Suite 225 Burlington, MA 01803 mribeiro@brinegroup.com Ph. (781) 272-3400 ext. 228 Fax (781) 494-3401 Visit our newly redesigned web site at www.brinegroup.com , the intelligent choice for staffing solutions. This electronic message contains information from Brine Group Staffing Solutions that may be privileged and confidential. The information is intended for the use of the addressee(s) only. If you are not an addressee, or received this email in error, note that any disclosure, copying, distribution, or use of the contents of this message is prohibited. If you have received this E-mail in error, please contact the sender immediately. ------------------------------ Message: 3 Date: Thu, 22 May 2008 09:23:09 -0500 From: "Burton, Lynn" Subject: RE: [Histonet] Hematoxylin shortage To: "KELLY BOYD" , "histonet" Message-ID: Content-Type: text/plain; charset="iso-8859-1" I was recently at a meeting for ISH, and this has been reported on this venue previously, and all vendors and some speakers confirmed this to be true. They do say that the shortage should clear up in a few months. They also said that stock piling was not a good idea. Better to stretch your supply as much as possible. Lynn Burton Animal Disease Lab Galesburg,Il ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of KELLY BOYD Sent: Thu 5/22/2008 8:51 AM To: histonet Subject: [Histonet] Hematoxylin shortage Can someone please tell me what is up with all the talk about a hematoxylin shortage? There is even a vendor telling their customers they need to stock up. Is this fact or rumor? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 Tele (252)-830-6866 Cell (252)-943-9527 Fax (252)-830-0032 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Thu, 22 May 2008 10:36:46 -0400 (EDT) From: "FU,DONGTAO" Subject: [Histonet] PH of Toluidine blue and aldehyde fuscin staining To: Histonet@lists.utsouthwestern.edu Message-ID: <1056368044.242941211467006107.JavaMail.osg@osgjas01.cns.ufl.edu> Content-Type: text/plain; format=flowed; charset=us-ascii Hi, all we will do some special staining of T blue and aldehyde fuscin. We will make some solutions by ourself. I notice some buffers need to measure PH value. But they are dyes, so you can not use dip paper. How do you measure them? Thank you, Ann ------------------------------ Message: 5 Date: Thu, 22 May 2008 10:36:58 -0400 From: "Nuernberger, Barb" Subject: [Histonet] osmium To: Message-ID: <66529604C8644845BB24FF6F6235664101FCC33C@EVS1.coj.net> Content-Type: text/plain; charset="us-ascii" I'm asking this on behalf of my histologist who has been asked by a pathologist to modify a protocol that she has from the AFIP manual. When doing an osmium fixation on formalin fixed tissue, is the 8 hour fixing time a minimum or can it sit overnight before continuing? Thank you for any help. Barb Nuernberger Chief Toxicologist Medical Examiner's Office 2100 Jefferson St. Jacksonville,FL 32206 (904)630-5470 BNuern@coj.net ------------------------------ Message: 6 Date: Thu, 22 May 2008 08:45:56 -0600 From: "Breeden, Sara" Subject: [Histonet] Manual BVD Procedure To: Cc: "Taylor, Flint" Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E631D@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" I have a visiting veterinary pathologist from Mexico this week; he is in need of a manual procedure for BVD. I run the Ventana system and am no help to him - could someone kindly share their manual BVD protocol with me? And perhaps a source for the antibody for same? My thanks! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ------------------------------ Message: 7 Date: Thu, 22 May 2008 10:49:01 -0400 From: crochieresteve@aol.com Subject: [Histonet] HIER to repair dried specimens? To: histonet@pathology.swmed.edu Message-ID: <8CA8A321B9E08F5-6B4-1295@webmail-nf15.sim.aol.com> Content-Type: text/plain; charset="us-ascii" I have accidentally stumbled on an unexplainable "by product" of HIER in citrate buffer, pH6.0. We had some specimens that had unfortunately air dried prior to fixation and processing and therefore looked terrible. The H&E was practically unreadable due to drying artifact. In an effort to see something worthwhile, IHC stains were ordered. The hematoxylin counterstain, following retrieval showed much better nuclear detail than the original H&E. Several additional slides were cut and stained with H&E following HIER. They were much better. This has been repeated on a few other specimens with similar results. Any ideas as to how or why this is working? thanks sc ------------------------------ Message: 8 Date: Thu, 22 May 2008 11:03:38 -0400 From: Philip Oshel Subject: Re: [Histonet] osmium To: "Nuernberger, Barb" Cc: Histonet@Pathology.swmed.edu Message-ID: Content-Type: text/plain; charset="us-ascii" ; format="flowed" Discounting regulations, generally osmium fixation can be done overnight at 4 deg C (refrigerator temperature). Mind, OsO4 is slow penetrating, and overnight might be required simply because of the size of the tissue blocks. Room temp ... mayyyyyybbbeee given the tissue requirements. But OsO4 makes tissues brittle and crunchy, so that usually isn't a good idea. Phil >I'm asking this on behalf of my histologist who has been asked by a >pathologist to modify a protocol that she has from the AFIP manual. >When doing an osmium fixation on formalin fixed tissue, is the 8 hour >fixing time a minimum or can it sit overnight before continuing? > >Thank you for any help. >Barb Nuernberger >Chief Toxicologist > > >Medical Examiner's Office >2100 Jefferson St. >Jacksonville,FL 32206 >(904)630-5470 >BNuern@coj.net -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576 ------------------------------ Message: 9 Date: Thu, 22 May 2008 11:11:54 -0400 From: "Cynthia Robinson" Subject: Re: [Histonet] Hematoxylin shortage To: "histonet" , "KELLY BOYD" , "Rene J Buesa" Message-ID: <483546EA.59BC.00AF.0@mercyhealth.com> Content-Type: text/plain; charset=UTF-8 I use Thermo Shandon as a supplier for my hematoxylin and just got an order placed on back order until the end of 2008. Cindi Robinson HT(ASCP) Mercy Medical Center-Sioux City Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes, SD 57049 >>> Rene J Buesa 5/22/2008 9:00 AM >>> Never pay attention to a vendor urging to stock up. It just may be a gimmick to increase sales/commissions. There is nothing concrete so far about that rumor. Ren?? J. KELLY BOYD wrote: Can someone please tell me what is up with all the talk about a hematoxylin shortage? There is even a vendor telling their customers they need to stock up. Is this fact or rumor? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 Tele (252)-830-6866 Cell (252)-943-9527 Fax (252)-830-0032 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Thu, 22 May 2008 16:19:51 +0100 From: "Orla Gallagher" Subject: [Histonet] Microtome blades jammed in dispensing box To: histonet@lists.utsouthwestern.edu Message-ID: <48359D26.20553.15909D5@localhost> Content-Type: text/plain; charset=US-ASCII Dear Histonetters, We've been having a problem over the past year or 2 with our microtome blades becoming jammed in the dispensing box after only a few have been used and wondered if this has happened to anyone else? Our refurbished building has a cooling system without humidity; would this cause the blades to become jammed? It's happened with Feather S35 & N35 blades and Accu Edge blades from different suppliers and in more than one lab in the building. Any advice on how to get the blades out would be much appreciated! Many thanks, Orla ****************************** Ms. Orla Gallagher Academic Unit of Bone Biology D Floor (DU20) Medical School Henry Wellcome Laboratories for Medical Research University of Sheffield Beech Hill Road Sheffield S10 2RX www.sheffield.ac.uk E-mail:o.m.gallagher@sheffield.ac.uk Tel: 0114 271 3783(lab) 0114 271 3337(office) Fax: 0114 271 1711 ------------------------------ Message: 11 Date: Thu, 22 May 2008 11:33:14 -0400 From: Roberta Horner Subject: RE: [Histonet] Microtome blades jammed in dispensing box To: Orla Gallagher , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I have had this happen several times. I found that if the dispenser button is pulled back so the next blade is ready to dispense I don't have it happen. When it does happen I have taken the dispenser apart carefully and separate the blades into a box lid. Roberta Horner HT/HTL Penn State University Animal Diagnostic Lab ------------------------------ Message: 12 Date: Thu, 22 May 2008 11:38:28 -0400 From: "Bryan Hewlett" Subject: Re: [Histonet] HIER to repair dried specimens? To: , Message-ID: <000501c8bc21$e4296580$6700a8c0@mainbox> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original It's called re-hydration! Similar techniques are used for partial restoration of mummified tissue for histological examination. Bryan \ ----- Original Message ----- From: To: Sent: Thursday, May 22, 2008 10:49 AM Subject: [Histonet] HIER to repair dried specimens? >I have accidentally stumbled on an unexplainable "by product" of HIER in >citrate buffer, pH6.0. > We had some specimens that had unfortunately air dried prior to fixation > and processing and therefore looked terrible. The H&E was practically > unreadable due to drying artifact. In an effort to see something > worthwhile, IHC stains were ordered. The hematoxylin counterstain, > following retrieval showed much better nuclear detail than the original > H&E. Several additional slides were cut and stained with H&E following > HIER. They were much better. This has been repeated on a few other > specimens with similar resu > lts. Any ideas as to how or why this is working? > thanks > sc > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 13 Date: Thu, 22 May 2008 10:39:53 -0500 From: "Salcedo, Rudy" Subject: [Histonet] Leica Peloris To: Message-ID: <0DE2321B1716814E9DF3B6753171E72201133632@EXCH9.tmh.tmhs> Content-Type: text/plain; charset="iso-8859-1" Histonet, Any labs out there using the Leica Peloris Processor, if so please send me some feed back on you experience with the Peloris. Thanks in advance. Rudy Salcedo Houston, Texas NOTICE: Our e-mail address at Methodist has changed to tmhs.org Methodist. Leading Medicine. Ranked No. 10 on FORTUNE magazine's list of the "100 Best Companies to Work For" in 2008 Named by U.S. News & World Report as one of "America's Best Hospitals" Designated as a Magnet hospital for excellence in nursing ***CONFIDENTIALITY NOTICE*** This e-mail is the property of The Methodist Hospital and/or its relevant affiliates and may contain confidential and privileged material for the sole use of the intended recipient(s). Any review, use, distribution or disclosure by others is strictly prohibited. If you are not the intended recipient (or authorized to receive for the recipient), please contact the sender and delete all copies of the message. Thank you. ------------------------------ Message: 14 Date: Thu, 22 May 2008 09:43:18 -0600 From: "Mary Abosso" Subject: RE: [Histonet] Richard Allan Hematoxylin problems To: "Shawn Leslie" , "Angela Bitting" Cc: HistoNet Message-ID: <43A451981FF6634795BE83B1B5494D6313647D@exchange.unipathllc.corp> Content-Type: text/plain; charset="iso-8859-1" I was the QA Histotechnologist at Richard-Allan and the best way to handle any product issues is to call in to Tech-Rite technical support who can help figure out what the issue is. Additonally, they will file a complaint with the QA department who will thouroughly investigate the retain sample from that lot, all of the chemicals used in the batch. Richard-Allan (Thermo-Fisher Scientific) is committed to the highest standard of quality and needs the customer feed back to continuously improve. Mary Abosso Unipath LLC. Denver, CO ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Shawn Leslie Sent: Wed 5/21/2008 2:30 PM To: Angela Bitting Cc: HistoNet Subject: Re: [Histonet] Richard Allan Hematoxylin problems We also use the Richard Allen 7211 but we haven't had any complaints. Maybe it's just a bad batch. Contact the vendor I am sure they will fix it.... Shawn Leslie HT (ASCP) Scientific Research Manager Anatomic Pathology University of Florida School of Veterinary Medicine 352-392-2235 ext 4555 >>> "Angela Bitting" 5/21/2008 3:18 PM >>> We have always changed our hematoxylin every Sunday night, and just filtered on the days in between. We've had no change in volumes. Now, all of a sudden, by Tuesday my hematoxylin staining is so pale that the docs are screaming. This has been going on for about two weeks now. We use Richard Allan Modified Harris Hematoxylin. Anyone else seen a change with that brand? I know there is supposed to be a "shortage" of hematoxylin right now. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> "Gayle Callis" 5/20/2008 4:00 PM >>> We use xylene to remove coverslips and do this work inside a hood. After coverslip is off and mounting media is gone with an extra xylene rinse, we go back to Clearite 3 to rinse off xylene and recoverslip. Anything to try an minimize exposure to xylene is done. Unfortunately, we found the substitute just didn't work for this job. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT ----- Original Message ----- From: "Patty Dunlop" To: Sent: Tuesday, May 20, 2008 1:20 PM Subject: [Histonet] xylene substitute recoverslipping > Hello Histonetters, > > I use Clear-Rite 3, a xylene substitute, and am having problems when I > need > to recoverslip. Currently, I just put the slide that needs recoverslipped > into a coplin jar of Clear-rite 3 at room temperature. The coverslip > really > doesn't like to come off (takes a week) and it tends to be a sticky, messy > process. Does anyone have a method for removing coverslips with a xylene > substitute? Should I use heat? > > Thanks, > Patty > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Thu, 22 May 2008 11:47:39 -0400 From: godsgalnow@aol.com Subject: [Histonet] now hiring To: histonet@lists.utsouthwestern.edu Message-ID: <8CA8A3A4C2B73E8-D34-22E4@FWM-D26.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Hey--- I know that you all have seen my posting for our salary ranges here and it has caused much disagreement, sorry about that!? However, I am now looking for a histotechnologist (Florida licensed as a technologist, or eligible), HT(ASCP), with QIHC preferred.? Extensive knowledge of IHC is needed, prefer molecular testing background.? MP certification would be great....... Excellent pay and bennies.......in sunny Tampa, Florida And the best part is....you get to work with me (ha ha). Roxanne ------------------------------ Message: 16 Date: Thu, 22 May 2008 08:48:51 -0700 (PDT) From: Paula Pierce Subject: [Histonet] Osmium To: Histonet Message-ID: <151803.29445.qm@web50108.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Are the specimens to be used for electron microscopy or fat stains? How big are they? Tell her to be very careful when using osmium. The fumes will fix your corneas. ?Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 631 N. Broadway Ave. Moore, OK 73160 405-570-6679 cell 405-759-3953 lab contact@excaliburpathology.com www.excaliburpathology.com ------------------------------ Message: 17 Date: Thu, 22 May 2008 11:51:48 -0400 From: crochieresteve@aol.com Subject: Re: [Histonet] HIER to repair dried specimens? To: bhewlett@cogeco.ca, histonet@pathology.swmed.edu Message-ID: <8CA8A3AE0814351-87C-14B7@webmail-nb18.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" I'm familiar with rehydrating mummy tissue from my years at AFIP. We used formol-glycol for several days prior to processing. I didn't think boiling slides in buffer would "repair" "cooked" tissue, but it seems to be so. Thanks s -----Original Message----- From: Bryan Hewlett To: histonet@pathology.swmed.edu; crochieresteve@aol.com Sent: Thu, 22 May 2008 11:38 am Subject: Re: [Histonet] HIER to repair dried specimens? It's called re-hydration!? Similar techniques are used for partial restoration of mummified tissue for histological examination.? ? Bryan? \? ? ----- Original Message ----- From: ? To: ? Sent: Thursday, May 22, 2008 10:49 AM? Subject: [Histonet] HIER to repair dried specimens?? ? >I have accidentally stumbled on an unexplainable "by product" of HIER in >citrate buffer, pH6.0.? > We had some specimens that had unfortunately air dried prior to fixation > and processing and therefore looked terrible. The H&E was practically > unreadable due to drying artifact. In an effort to see something > worthwhile, IHC stains were ordered. The hematoxylin counterstain, > following retrieval showed much better nuclear detail than the original > H&E. Several additional slides were cut and stained with H&E following > HIER. They were much better. This has been repeated on a few other > specimens with similar resu? > lts. Any ideas as to how or why this is working?? > thanks? > sc? > _______________________________________________? > Histonet mailing list? > Histonet@lists.utsouthwestern.edu? > http://lists.utsouthwestern.edu/mailman/listinfo/histonet? > ? ------------------------------ Message: 18 Date: Thu, 22 May 2008 08:53:36 -0700 (PDT) From: Paula Pierce Subject: [Histonet] Microtome blades jammed in dispenser box To: Histonet Message-ID: <516735.64474.qm@web50109.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I am having the same problem. The tab that grabs the hole in the blades to push them forward tends to wear more than in the past and does not grab after a few have been dispensed. I have taken a very fine pair of forceps and helped the blade along and when that no loner works because the blade does not move at all I took the box apart. ?Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 631 N. Broadway Ave. Moore, OK 73160 405-570-6679 cell 405-759-3953 lab contact@excaliburpathology.com www.excaliburpathology.com ------------------------------ Message: 19 Date: Thu, 22 May 2008 11:18:45 -0500 From: "Mike Pence" Subject: RE: [Histonet] Leica Peloris To: "Salcedo, Rudy" , Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3849@IS-E2K3.grhs.net> Content-Type: text/plain; charset="us-ascii" I just purchased a Peloris about 3 months ago. Love it. I can process all thru the day and it has made a huge impact on turn around times. We cut fewer blocks thru out the day and I think it processes fatty specimens better. I have a few small issues, but I have been very happy with the processor. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Salcedo, Rudy Sent: Thursday, May 22, 2008 10:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Peloris Histonet, Any labs out there using the Leica Peloris Processor, if so please send me some feed back on you experience with the Peloris. Thanks in advance. Rudy Salcedo Houston, Texas NOTICE: Our e-mail address at Methodist has changed to tmhs.org Methodist. Leading Medicine. Ranked No. 10 on FORTUNE magazine's list of the "100 Best Companies to Work For" in 2008 Named by U.S. News & World Report as one of "America's Best Hospitals" Designated as a Magnet hospital for excellence in nursing ***CONFIDENTIALITY NOTICE*** This e-mail is the property of The Methodist Hospital and/or its relevant affiliates and may contain confidential and privileged material for the sole use of the intended recipient(s). Any review, use, distribution or disclosure by others is strictly prohibited. If you are not the intended recipient (or authorized to receive for the recipient), please contact the sender and delete all copies of the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Thu, 22 May 2008 12:21:46 -0400 From: "Terri Brown" Subject: RE: [Histonet] Hematoxylin shortage To: "Cynthia Robinson" , "histonet" , "KELLY BOYD" , "Rene J Buesa" Message-ID: <8CEB6DA1A3F35743800669D4CFE21F7D03F8FFE0@NSMXMS04.northside.local> Content-Type: text/plain; charset="iso-8859-1" I heard that this was going to happen and ordered the powder so we could make our own. It is on backorder too. I ordered it back in April. Terri Brown, HT (ASCP) Northside Hospital-Atlanta, Ga. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Robinson Sent: Thursday, May 22, 2008 11:12 AM To: histonet; KELLY BOYD; Rene J Buesa Subject: Re: [Histonet] Hematoxylin shortage I use Thermo Shandon as a supplier for my hematoxylin and just got an order placed on back order until the end of 2008. Cindi Robinson HT(ASCP) Mercy Medical Center-Sioux City Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes, SD 57049 >>> Rene J Buesa 5/22/2008 9:00 AM >>> Never pay attention to a vendor urging to stock up. It just may be a gimmick to increase sales/commissions. There is nothing concrete so far about that rumor. Ren? J. KELLY BOYD wrote: Can someone please tell me what is up with all the talk about a hematoxylin shortage? There is even a vendor telling their customers they need to stock up. Is this fact or rumor? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 Tele (252)-830-6866 Cell (252)-943-9527 Fax (252)-830-0032 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 54, Issue 35 **************************************** From kevinpe <@t> mail.med.upenn.edu Thu May 22 15:11:44 2008 From: kevinpe <@t> mail.med.upenn.edu (Kevin Egan) Date: Thu May 22 15:11:49 2008 Subject: [Histonet] Poor DAPI stain in old specimens? Message-ID: <1211487104.4835d380e57f7@webmail.pobox.upenn.edu> Hello all, This list has been very helpful to new people in the field. I work in Orthopaedic surgery and I am having trouble with immunofluorescent staining bone sections. It's not so much the autofluorescence; I avoid the GFP channel at all costs and can distinguish real signal from background in the Green and IR channels pretty well. The problem I recently have been running into is no DAPI staining in my sections. These sections are heart valves removed from older people with end stage calcified heart valves. The dapi staining is really weak or not even there, all I see is lots of background in the UV filter. I can see where cells probably are, based on the round shape and fluorescent signal in other channels, but it's indistinguishable from non-cellular parts. I know the DAPI I am using works, I have used it successfully in younger bone specimens. I checked the sample under a confocal microscope, and it seems pretty clear that the DAPI is not staining the nuclei, instead of it just staining everything. I recently learned that a colleague in the lab here is having similar problems with his old bone specimens. A recently collected specimen which was quickly fixed, processed, and embedded in parrafin is giving the same poor DAPI staining. This is in stark contrast to bone specimens he has collected from younger individuals, where the DAPI stains quite well. We can clearly see cells in H&E/Saffranin-O stains, but the cells don't pickup any DAPI stain. In both cases, the samples are formalin fixed and embedded with either parrafin or paraplast, and then cut at 7 microns. Does anyone know why DAPI would not work in older tissue samples? Has anyone else experienced this? Thank you very much, Kevin -- Kevin Egan Research Specialist University of Pennsylvania School of Medicine From LSebree <@t> uwhealth.org Thu May 22 15:12:12 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Thu May 22 15:12:23 2008 Subject: [Histonet] p53 In-Reply-To: <328CBAE62F31C642B422970E879DFADC03A36954@pcwex01> Message-ID: Hi Jean, We use Biocare Medical's p53, clone DO-7. It works well for us at 1:50 on our VMS instruments but we do use a pretty long on-line HIER and an amplification step on 2 of our 3 instruments. Take care, Linda Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean Sent: Thursday, May 22, 2008 3:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p53 Hi everyone, I am wondering what clone of p53 labs are using. I currently use Dako's DO-7 clone but am also testing NeoMarker's rabbit monoclonal SP5. I've gotten very different results comparing the two and would like to know what the majority of labs are using. Thanks! Jean Taylor HT(ASCP)QIHC Meriter Labs Madison, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, May 22, 2008 12:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 54, Issue 35 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Factor 13a (Sebree Linda A.) 2. Histology Supervisor - IHC (Melissa Ribeiro) 3. RE: Hematoxylin shortage (Burton, Lynn) 4. PH of Toluidine blue and aldehyde fuscin staining (FU,DONGTAO) 5. osmium (Nuernberger, Barb) 6. Manual BVD Procedure (Breeden, Sara) 7. HIER to repair dried specimens? (crochieresteve@aol.com) 8. Re: osmium (Philip Oshel) 9. Re: Hematoxylin shortage (Cynthia Robinson) 10. Microtome blades jammed in dispensing box (Orla Gallagher) 11. RE: Microtome blades jammed in dispensing box (Roberta Horner) 12. Re: HIER to repair dried specimens? (Bryan Hewlett) 13. Leica Peloris (Salcedo, Rudy) 14. RE: Richard Allan Hematoxylin problems (Mary Abosso) 15. now hiring (godsgalnow@aol.com) 16. Osmium (Paula Pierce) 17. Re: HIER to repair dried specimens? (crochieresteve@aol.com) 18. Microtome blades jammed in dispenser box (Paula Pierce) 19. RE: Leica Peloris (Mike Pence) 20. RE: Hematoxylin shortage (Terri Brown) ---------------------------------------------------------------------- Message: 1 Date: Thu, 22 May 2008 09:10:01 -0500 From: "Sebree Linda A." Subject: [Histonet] Factor 13a To: "Histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" Good morning everyone, My latest lot of Factor 13a, from a reputable vendor, has "pooped out" It tests out fine at their facility but is much weaker on my Benchmark, XT and NexES, with DAB detection, than the previous lot and is basically negative with Alk. Phos. Red detection. So my question is, which F13a are people using reliably on Ventana instruments? Thanks for the help, Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 ------------------------------ Message: 2 Date: Thu, 22 May 2008 10:24:22 -0400 From: "Melissa Ribeiro" Subject: [Histonet] Histology Supervisor - IHC To: Message-ID: <0D88BB18E51FE3449EB2D2F505936A7A4ADB9C@brinedc.brine.local> Content-Type: text/plain; charset="US-ASCII" Our firm has been retained to identify a candidate with a strong IHC background for a Histology Supervisor position in a Massachusetts Teaching Hospital. Any qualified and interested candidates, please feel free to send resumes to mribeiro@brinegroup.com or call (781) 272-3400 Melissa Ribeiro Healthcare Division Brine Group Staffing Solutions 20 Mall Road, Suite 225 Burlington, MA 01803 mribeiro@brinegroup.com Ph. (781) 272-3400 ext. 228 Fax (781) 494-3401 Visit our newly redesigned web site at www.brinegroup.com , the intelligent choice for staffing solutions. This electronic message contains information from Brine Group Staffing Solutions that may be privileged and confidential. The information is intended for the use of the addressee(s) only. If you are not an addressee, or received this email in error, note that any disclosure, copying, distribution, or use of the contents of this message is prohibited. If you have received this E-mail in error, please contact the sender immediately. ------------------------------ Message: 3 Date: Thu, 22 May 2008 09:23:09 -0500 From: "Burton, Lynn" Subject: RE: [Histonet] Hematoxylin shortage To: "KELLY BOYD" , "histonet" Message-ID: Content-Type: text/plain; charset="iso-8859-1" I was recently at a meeting for ISH, and this has been reported on this venue previously, and all vendors and some speakers confirmed this to be true. They do say that the shortage should clear up in a few months. They also said that stock piling was not a good idea. Better to stretch your supply as much as possible. Lynn Burton Animal Disease Lab Galesburg,Il ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of KELLY BOYD Sent: Thu 5/22/2008 8:51 AM To: histonet Subject: [Histonet] Hematoxylin shortage Can someone please tell me what is up with all the talk about a hematoxylin shortage? There is even a vendor telling their customers they need to stock up. Is this fact or rumor? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 Tele (252)-830-6866 Cell (252)-943-9527 Fax (252)-830-0032 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Thu, 22 May 2008 10:36:46 -0400 (EDT) From: "FU,DONGTAO" Subject: [Histonet] PH of Toluidine blue and aldehyde fuscin staining To: Histonet@lists.utsouthwestern.edu Message-ID: <1056368044.242941211467006107.JavaMail.osg@osgjas01.cns.ufl.edu> Content-Type: text/plain; format=flowed; charset=us-ascii Hi, all we will do some special staining of T blue and aldehyde fuscin. We will make some solutions by ourself. I notice some buffers need to measure PH value. But they are dyes, so you can not use dip paper. How do you measure them? Thank you, Ann ------------------------------ Message: 5 Date: Thu, 22 May 2008 10:36:58 -0400 From: "Nuernberger, Barb" Subject: [Histonet] osmium To: Message-ID: <66529604C8644845BB24FF6F6235664101FCC33C@EVS1.coj.net> Content-Type: text/plain; charset="us-ascii" I'm asking this on behalf of my histologist who has been asked by a pathologist to modify a protocol that she has from the AFIP manual. When doing an osmium fixation on formalin fixed tissue, is the 8 hour fixing time a minimum or can it sit overnight before continuing? Thank you for any help. Barb Nuernberger Chief Toxicologist Medical Examiner's Office 2100 Jefferson St. Jacksonville,FL 32206 (904)630-5470 BNuern@coj.net ------------------------------ Message: 6 Date: Thu, 22 May 2008 08:45:56 -0600 From: "Breeden, Sara" Subject: [Histonet] Manual BVD Procedure To: Cc: "Taylor, Flint" Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E631D@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" I have a visiting veterinary pathologist from Mexico this week; he is in need of a manual procedure for BVD. I run the Ventana system and am no help to him - could someone kindly share their manual BVD protocol with me? And perhaps a source for the antibody for same? My thanks! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ------------------------------ Message: 7 Date: Thu, 22 May 2008 10:49:01 -0400 From: crochieresteve@aol.com Subject: [Histonet] HIER to repair dried specimens? To: histonet@pathology.swmed.edu Message-ID: <8CA8A321B9E08F5-6B4-1295@webmail-nf15.sim.aol.com> Content-Type: text/plain; charset="us-ascii" I have accidentally stumbled on an unexplainable "by product" of HIER in citrate buffer, pH6.0. We had some specimens that had unfortunately air dried prior to fixation and processing and therefore looked terrible. The H&E was practically unreadable due to drying artifact. In an effort to see something worthwhile, IHC stains were ordered. The hematoxylin counterstain, following retrieval showed much better nuclear detail than the original H&E. Several additional slides were cut and stained with H&E following HIER. They were much better. This has been repeated on a few other specimens with similar results. Any ideas as to how or why this is working? thanks sc ------------------------------ Message: 8 Date: Thu, 22 May 2008 11:03:38 -0400 From: Philip Oshel Subject: Re: [Histonet] osmium To: "Nuernberger, Barb" Cc: Histonet@Pathology.swmed.edu Message-ID: Content-Type: text/plain; charset="us-ascii" ; format="flowed" Discounting regulations, generally osmium fixation can be done overnight at 4 deg C (refrigerator temperature). Mind, OsO4 is slow penetrating, and overnight might be required simply because of the size of the tissue blocks. Room temp ... mayyyyyybbbeee given the tissue requirements. But OsO4 makes tissues brittle and crunchy, so that usually isn't a good idea. Phil >I'm asking this on behalf of my histologist who has been asked by a >pathologist to modify a protocol that she has from the AFIP manual. >When doing an osmium fixation on formalin fixed tissue, is the 8 hour >fixing time a minimum or can it sit overnight before continuing? > >Thank you for any help. >Barb Nuernberger >Chief Toxicologist > > >Medical Examiner's Office >2100 Jefferson St. >Jacksonville,FL 32206 >(904)630-5470 >BNuern@coj.net -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576 ------------------------------ Message: 9 Date: Thu, 22 May 2008 11:11:54 -0400 From: "Cynthia Robinson" Subject: Re: [Histonet] Hematoxylin shortage To: "histonet" , "KELLY BOYD" , "Rene J Buesa" Message-ID: <483546EA.59BC.00AF.0@mercyhealth.com> Content-Type: text/plain; charset=UTF-8 I use Thermo Shandon as a supplier for my hematoxylin and just got an order placed on back order until the end of 2008. Cindi Robinson HT(ASCP) Mercy Medical Center-Sioux City Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes, SD 57049 >>> Rene J Buesa 5/22/2008 9:00 AM >>> Never pay attention to a vendor urging to stock up. It just may be a gimmick to increase sales/commissions. There is nothing concrete so far about that rumor. Ren?? J. KELLY BOYD wrote: Can someone please tell me what is up with all the talk about a hematoxylin shortage? There is even a vendor telling their customers they need to stock up. Is this fact or rumor? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 Tele (252)-830-6866 Cell (252)-943-9527 Fax (252)-830-0032 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Thu, 22 May 2008 16:19:51 +0100 From: "Orla Gallagher" Subject: [Histonet] Microtome blades jammed in dispensing box To: histonet@lists.utsouthwestern.edu Message-ID: <48359D26.20553.15909D5@localhost> Content-Type: text/plain; charset=US-ASCII Dear Histonetters, We've been having a problem over the past year or 2 with our microtome blades becoming jammed in the dispensing box after only a few have been used and wondered if this has happened to anyone else? Our refurbished building has a cooling system without humidity; would this cause the blades to become jammed? It's happened with Feather S35 & N35 blades and Accu Edge blades from different suppliers and in more than one lab in the building. Any advice on how to get the blades out would be much appreciated! Many thanks, Orla ****************************** Ms. Orla Gallagher Academic Unit of Bone Biology D Floor (DU20) Medical School Henry Wellcome Laboratories for Medical Research University of Sheffield Beech Hill Road Sheffield S10 2RX www.sheffield.ac.uk E-mail:o.m.gallagher@sheffield.ac.uk Tel: 0114 271 3783(lab) 0114 271 3337(office) Fax: 0114 271 1711 ------------------------------ Message: 11 Date: Thu, 22 May 2008 11:33:14 -0400 From: Roberta Horner Subject: RE: [Histonet] Microtome blades jammed in dispensing box To: Orla Gallagher , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I have had this happen several times. I found that if the dispenser button is pulled back so the next blade is ready to dispense I don't have it happen. When it does happen I have taken the dispenser apart carefully and separate the blades into a box lid. Roberta Horner HT/HTL Penn State University Animal Diagnostic Lab ------------------------------ Message: 12 Date: Thu, 22 May 2008 11:38:28 -0400 From: "Bryan Hewlett" Subject: Re: [Histonet] HIER to repair dried specimens? To: , Message-ID: <000501c8bc21$e4296580$6700a8c0@mainbox> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original It's called re-hydration! Similar techniques are used for partial restoration of mummified tissue for histological examination. Bryan \ ----- Original Message ----- From: To: Sent: Thursday, May 22, 2008 10:49 AM Subject: [Histonet] HIER to repair dried specimens? >I have accidentally stumbled on an unexplainable "by product" of HIER in >citrate buffer, pH6.0. > We had some specimens that had unfortunately air dried prior to fixation > and processing and therefore looked terrible. The H&E was practically > unreadable due to drying artifact. In an effort to see something > worthwhile, IHC stains were ordered. The hematoxylin counterstain, > following retrieval showed much better nuclear detail than the original > H&E. Several additional slides were cut and stained with H&E following > HIER. They were much better. This has been repeated on a few other > specimens with similar resu > lts. Any ideas as to how or why this is working? > thanks > sc > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 13 Date: Thu, 22 May 2008 10:39:53 -0500 From: "Salcedo, Rudy" Subject: [Histonet] Leica Peloris To: Message-ID: <0DE2321B1716814E9DF3B6753171E72201133632@EXCH9.tmh.tmhs> Content-Type: text/plain; charset="iso-8859-1" Histonet, Any labs out there using the Leica Peloris Processor, if so please send me some feed back on you experience with the Peloris. Thanks in advance. Rudy Salcedo Houston, Texas NOTICE: Our e-mail address at Methodist has changed to tmhs.org Methodist. Leading Medicine. Ranked No. 10 on FORTUNE magazine's list of the "100 Best Companies to Work For" in 2008 Named by U.S. News & World Report as one of "America's Best Hospitals" Designated as a Magnet hospital for excellence in nursing ***CONFIDENTIALITY NOTICE*** This e-mail is the property of The Methodist Hospital and/or its relevant affiliates and may contain confidential and privileged material for the sole use of the intended recipient(s). Any review, use, distribution or disclosure by others is strictly prohibited. If you are not the intended recipient (or authorized to receive for the recipient), please contact the sender and delete all copies of the message. Thank you. ------------------------------ Message: 14 Date: Thu, 22 May 2008 09:43:18 -0600 From: "Mary Abosso" Subject: RE: [Histonet] Richard Allan Hematoxylin problems To: "Shawn Leslie" , "Angela Bitting" Cc: HistoNet Message-ID: <43A451981FF6634795BE83B1B5494D6313647D@exchange.unipathllc.corp> Content-Type: text/plain; charset="iso-8859-1" I was the QA Histotechnologist at Richard-Allan and the best way to handle any product issues is to call in to Tech-Rite technical support who can help figure out what the issue is. Additonally, they will file a complaint with the QA department who will thouroughly investigate the retain sample from that lot, all of the chemicals used in the batch. Richard-Allan (Thermo-Fisher Scientific) is committed to the highest standard of quality and needs the customer feed back to continuously improve. Mary Abosso Unipath LLC. Denver, CO ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Shawn Leslie Sent: Wed 5/21/2008 2:30 PM To: Angela Bitting Cc: HistoNet Subject: Re: [Histonet] Richard Allan Hematoxylin problems We also use the Richard Allen 7211 but we haven't had any complaints. Maybe it's just a bad batch. Contact the vendor I am sure they will fix it.... Shawn Leslie HT (ASCP) Scientific Research Manager Anatomic Pathology University of Florida School of Veterinary Medicine 352-392-2235 ext 4555 >>> "Angela Bitting" 5/21/2008 3:18 PM >>> We have always changed our hematoxylin every Sunday night, and just filtered on the days in between. We've had no change in volumes. Now, all of a sudden, by Tuesday my hematoxylin staining is so pale that the docs are screaming. This has been going on for about two weeks now. We use Richard Allan Modified Harris Hematoxylin. Anyone else seen a change with that brand? I know there is supposed to be a "shortage" of hematoxylin right now. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> "Gayle Callis" 5/20/2008 4:00 PM >>> We use xylene to remove coverslips and do this work inside a hood. After coverslip is off and mounting media is gone with an extra xylene rinse, we go back to Clearite 3 to rinse off xylene and recoverslip. Anything to try an minimize exposure to xylene is done. Unfortunately, we found the substitute just didn't work for this job. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT ----- Original Message ----- From: "Patty Dunlop" To: Sent: Tuesday, May 20, 2008 1:20 PM Subject: [Histonet] xylene substitute recoverslipping > Hello Histonetters, > > I use Clear-Rite 3, a xylene substitute, and am having problems when I > need > to recoverslip. Currently, I just put the slide that needs recoverslipped > into a coplin jar of Clear-rite 3 at room temperature. The coverslip > really > doesn't like to come off (takes a week) and it tends to be a sticky, messy > process. Does anyone have a method for removing coverslips with a xylene > substitute? Should I use heat? > > Thanks, > Patty > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Thu, 22 May 2008 11:47:39 -0400 From: godsgalnow@aol.com Subject: [Histonet] now hiring To: histonet@lists.utsouthwestern.edu Message-ID: <8CA8A3A4C2B73E8-D34-22E4@FWM-D26.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Hey--- I know that you all have seen my posting for our salary ranges here and it has caused much disagreement, sorry about that!? However, I am now looking for a histotechnologist (Florida licensed as a technologist, or eligible), HT(ASCP), with QIHC preferred.? Extensive knowledge of IHC is needed, prefer molecular testing background.? MP certification would be great....... Excellent pay and bennies.......in sunny Tampa, Florida And the best part is....you get to work with me (ha ha). Roxanne ------------------------------ Message: 16 Date: Thu, 22 May 2008 08:48:51 -0700 (PDT) From: Paula Pierce Subject: [Histonet] Osmium To: Histonet Message-ID: <151803.29445.qm@web50108.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Are the specimens to be used for electron microscopy or fat stains? How big are they? Tell her to be very careful when using osmium. The fumes will fix your corneas. ?Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 631 N. Broadway Ave. Moore, OK 73160 405-570-6679 cell 405-759-3953 lab contact@excaliburpathology.com www.excaliburpathology.com ------------------------------ Message: 17 Date: Thu, 22 May 2008 11:51:48 -0400 From: crochieresteve@aol.com Subject: Re: [Histonet] HIER to repair dried specimens? To: bhewlett@cogeco.ca, histonet@pathology.swmed.edu Message-ID: <8CA8A3AE0814351-87C-14B7@webmail-nb18.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" I'm familiar with rehydrating mummy tissue from my years at AFIP. We used formol-glycol for several days prior to processing. I didn't think boiling slides in buffer would "repair" "cooked" tissue, but it seems to be so. Thanks s -----Original Message----- From: Bryan Hewlett To: histonet@pathology.swmed.edu; crochieresteve@aol.com Sent: Thu, 22 May 2008 11:38 am Subject: Re: [Histonet] HIER to repair dried specimens? It's called re-hydration!? Similar techniques are used for partial restoration of mummified tissue for histological examination.? ? Bryan? \? ? ----- Original Message ----- From: ? To: ? Sent: Thursday, May 22, 2008 10:49 AM? Subject: [Histonet] HIER to repair dried specimens?? ? >I have accidentally stumbled on an unexplainable "by product" of HIER in >citrate buffer, pH6.0.? > We had some specimens that had unfortunately air dried prior to fixation > and processing and therefore looked terrible. The H&E was practically > unreadable due to drying artifact. In an effort to see something > worthwhile, IHC stains were ordered. The hematoxylin counterstain, > following retrieval showed much better nuclear detail than the original > H&E. Several additional slides were cut and stained with H&E following > HIER. They were much better. This has been repeated on a few other > specimens with similar resu? > lts. Any ideas as to how or why this is working?? > thanks? > sc? > _______________________________________________? > Histonet mailing list? > Histonet@lists.utsouthwestern.edu? > http://lists.utsouthwestern.edu/mailman/listinfo/histonet? > ? ------------------------------ Message: 18 Date: Thu, 22 May 2008 08:53:36 -0700 (PDT) From: Paula Pierce Subject: [Histonet] Microtome blades jammed in dispenser box To: Histonet Message-ID: <516735.64474.qm@web50109.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I am having the same problem. The tab that grabs the hole in the blades to push them forward tends to wear more than in the past and does not grab after a few have been dispensed. I have taken a very fine pair of forceps and helped the blade along and when that no loner works because the blade does not move at all I took the box apart. ?Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 631 N. Broadway Ave. Moore, OK 73160 405-570-6679 cell 405-759-3953 lab contact@excaliburpathology.com www.excaliburpathology.com ------------------------------ Message: 19 Date: Thu, 22 May 2008 11:18:45 -0500 From: "Mike Pence" Subject: RE: [Histonet] Leica Peloris To: "Salcedo, Rudy" , Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3849@IS-E2K3.grhs.net> Content-Type: text/plain; charset="us-ascii" I just purchased a Peloris about 3 months ago. Love it. I can process all thru the day and it has made a huge impact on turn around times. We cut fewer blocks thru out the day and I think it processes fatty specimens better. I have a few small issues, but I have been very happy with the processor. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Salcedo, Rudy Sent: Thursday, May 22, 2008 10:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Peloris Histonet, Any labs out there using the Leica Peloris Processor, if so please send me some feed back on you experience with the Peloris. Thanks in advance. Rudy Salcedo Houston, Texas NOTICE: Our e-mail address at Methodist has changed to tmhs.org Methodist. Leading Medicine. Ranked No. 10 on FORTUNE magazine's list of the "100 Best Companies to Work For" in 2008 Named by U.S. News & World Report as one of "America's Best Hospitals" Designated as a Magnet hospital for excellence in nursing ***CONFIDENTIALITY NOTICE*** This e-mail is the property of The Methodist Hospital and/or its relevant affiliates and may contain confidential and privileged material for the sole use of the intended recipient(s). Any review, use, distribution or disclosure by others is strictly prohibited. If you are not the intended recipient (or authorized to receive for the recipient), please contact the sender and delete all copies of the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Thu, 22 May 2008 12:21:46 -0400 From: "Terri Brown" Subject: RE: [Histonet] Hematoxylin shortage To: "Cynthia Robinson" , "histonet" , "KELLY BOYD" , "Rene J Buesa" Message-ID: <8CEB6DA1A3F35743800669D4CFE21F7D03F8FFE0@NSMXMS04.northside.local> Content-Type: text/plain; charset="iso-8859-1" I heard that this was going to happen and ordered the powder so we could make our own. It is on backorder too. I ordered it back in April. Terri Brown, HT (ASCP) Northside Hospital-Atlanta, Ga. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cynthia Robinson Sent: Thursday, May 22, 2008 11:12 AM To: histonet; KELLY BOYD; Rene J Buesa Subject: Re: [Histonet] Hematoxylin shortage I use Thermo Shandon as a supplier for my hematoxylin and just got an order placed on back order until the end of 2008. Cindi Robinson HT(ASCP) Mercy Medical Center-Sioux City Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes, SD 57049 >>> Rene J Buesa 5/22/2008 9:00 AM >>> Never pay attention to a vendor urging to stock up. It just may be a gimmick to increase sales/commissions. There is nothing concrete so far about that rumor. Ren? J. KELLY BOYD wrote: Can someone please tell me what is up with all the talk about a hematoxylin shortage? There is even a vendor telling their customers they need to stock up. Is this fact or rumor? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 Tele (252)-830-6866 Cell (252)-943-9527 Fax (252)-830-0032 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 54, Issue 35 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmjohnson34 <@t> hotmail.com Thu May 22 15:43:34 2008 From: jmjohnson34 <@t> hotmail.com (Jennifer Johnson) Date: Thu May 22 15:43:43 2008 Subject: [Histonet] (no subject) Message-ID: Dear Netters, Last year I was planning a vacation to Albuquerque, NM and Sara Breeden gave me the most excellent list of things to do! I figured I would try it again. Next Wednesday I will be leaving for a two week vacation to Fiji, New Zealand, Australia, and Hawaii. Any suggestions from you locals? Please reply off list so as not to clog up the histonet. Thanks, Jennifer Johnson, HTL (ASCP) _________________________________________________________________ Change the world with e-mail. Join the i?m Initiative from Microsoft. http://im.live.com/Messenger/IM/Join/Default.aspx?source=EML_WL_ChangeWorld From brett_connolly <@t> merck.com Thu May 22 15:47:46 2008 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Thu May 22 15:47:57 2008 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: <63EA0607835FBA4689CEA9EA8B482692011BAC3F@usctmx1141.merck.com> I suggest you take the rest of us with you !! Brett Brett M. Connolly, Ph.D. Research Fellow MRL, Imaging Research Merck & Co., Inc. WP-44K PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Johnson Sent: Thursday, May 22, 2008 4:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Dear Netters, Last year I was planning a vacation to Albuquerque, NM and Sara Breeden gave me the most excellent list of things to do! I figured I would try it again. Next Wednesday I will be leaving for a two week vacation to Fiji, New Zealand, Australia, and Hawaii. Any suggestions from you locals? Please reply off list so as not to clog up the histonet. Thanks, Jennifer Johnson, HTL (ASCP) _________________________________________________________________ Change the world with e-mail. Join the i'm Initiative from Microsoft. http://im.live.com/Messenger/IM/Join/Default.aspx?source=EML_WL_ChangeWo rld_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From pmarcum <@t> vet.upenn.edu Thu May 22 16:05:25 2008 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Thu May 22 16:05:34 2008 Subject: [Histonet] (no subject) In-Reply-To: <63EA0607835FBA4689CEA9EA8B482692011BAC3F@usctmx1141.merck. com> References: <63EA0607835FBA4689CEA9EA8B482692011BAC3F@usctmx1141.merck.com> Message-ID: <6.2.5.6.2.20080522170454.01cc51a0@vet.upenn.edu> Absolutely Great Idea, Brett. She pays right?? Pam Marcum At 04:47 PM 5/22/2008, Connolly, Brett M wrote: >I suggest you take the rest of us with you !! > >Brett > >Brett M. Connolly, Ph.D. >Research Fellow >MRL, Imaging Research >Merck & Co., Inc. >WP-44K >PO Box 4 >West Point, PA 19486 >PH 215-652-2501 >fax. 215-993-6803 >e-mail. brett_connolly@merck.com > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer >Johnson >Sent: Thursday, May 22, 2008 4:44 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] (no subject) > > >Dear Netters, > >Last year I was planning a vacation to Albuquerque, NM and Sara Breeden >gave me the most excellent list of things to do! I figured I would try >it again. Next Wednesday I will be leaving for a two week vacation to >Fiji, New Zealand, Australia, and Hawaii. Any suggestions from you >locals? Please reply off list so as not to clog up the histonet. >Thanks, Jennifer Johnson, HTL (ASCP) >_________________________________________________________________ >Change the world with e-mail. Join the i'm Initiative from Microsoft. >http://im.live.com/Messenger/IM/Join/Default.aspx?source=EML_WL_ChangeWo >rld_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >Notice: This e-mail message, together with any attachments, >contains information of Merck & Co., Inc. (One Merck Drive, >Whitehouse Station, New Jersey, USA 08889), and/or its affiliates >(which may be known outside the United States as Merck Frosst, Merck >Sharp & Dohme or MSD and in Japan, as Banyu - direct contact >information for affiliates is available at >http://www.merck.com/contact/contacts.html) that may be >confidential, proprietary copyrighted and/or legally privileged. It >is intended solely for the use of the individual or entity named on >this message. If you are not the intended recipient, and have >received this message in error, please notify us immediately by >reply e-mail and then delete it from your system. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From Sharon.Davis-Devine <@t> carle.com Thu May 22 16:13:37 2008 From: Sharon.Davis-Devine <@t> carle.com (Sharon.Davis-Devine) Date: Thu May 22 16:13:49 2008 Subject: [Histonet] Validation Message-ID: <44780C571F28624DBB446DE55C4D733A021E09A6@EXCHANGEBE1.carle.com> We are currently in the process of switching from a Dako immuno stainer to the Ventana system. We need to do a validation for the new system but need ideas from everyone out there who has done a validation to give us some pointers on how to accomplish this. Any information is greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com From Heidi.Miers <@t> nau.edu Thu May 22 17:27:01 2008 From: Heidi.Miers <@t> nau.edu (Heidi Miers) Date: Thu May 22 17:27:09 2008 Subject: [Histonet] Skin biopsies Message-ID: <4835F335.2020509@nau.edu> Does anyone have any advice on tissue processing times for 0.5 to 1 mm thick skin biopsies for IHC? Thanks to all in advance, Heidi From anitaibsc <@t> aol.com Thu May 22 17:51:10 2008 From: anitaibsc <@t> aol.com (anitaibsc@aol.com) Date: Thu May 22 17:51:25 2008 Subject: [Histonet] Hematoxylin shortage Message-ID: <8CA8A757697861D-8C4-8D3@webmail-nd19.sysops.aol.com> Hello Netteres, The information?I provided was to assist with a specific product and was not intended as a mass, commercial advertisement. IBSC makes variety of IHC products not just Hematoxylin. Anita From jnocito <@t> satx.rr.com Thu May 22 18:51:41 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu May 22 18:51:47 2008 Subject: [Histonet] Leica Peloris References: <57BE698966D5C54EAE8612E8941D768303019248@EXCHANGE3.huntingtonhospital.com> Message-ID: <00c001c8bc66$c8d0c3b0$0302a8c0@yourxhtr8hvc4p> we just purchased two, with a third on the way. We took off the xylene and the tissue has processed better. We had to monkey around with our fatty specimens a little. We run a 4 hour and an 8 hour xylene-free program. Next week, we try the 1.5 hour xylene free on real specimens. We are trying to process throughout the day. So far, I like them. But, need to see the response time if a repair needs to be made. This company has not had a good track record in the past. JTT ----- Original Message ----- From: "Laurie Colbert" To: "Mike Pence" ; "Salcedo, Rudy" ; Sent: Thursday, May 22, 2008 1:16 PM Subject: RE: [Histonet] Leica Peloris Do you use xylene on the Peloris? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Thursday, May 22, 2008 9:19 AM To: Salcedo, Rudy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica Peloris I just purchased a Peloris about 3 months ago. Love it. I can process all thru the day and it has made a huge impact on turn around times. We cut fewer blocks thru out the day and I think it processes fatty specimens better. I have a few small issues, but I have been very happy with the processor. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Salcedo, Rudy Sent: Thursday, May 22, 2008 10:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Peloris Histonet, Any labs out there using the Leica Peloris Processor, if so please send me some feed back on you experience with the Peloris. Thanks in advance. Rudy Salcedo Houston, Texas NOTICE: Our e-mail address at Methodist has changed to tmhs.org Methodist. Leading Medicine. Ranked No. 10 on FORTUNE magazine's list of the "100 Best Companies to Work For" in 2008 Named by U.S. News & World Report as one of "America's Best Hospitals" Designated as a Magnet hospital for excellence in nursing ***CONFIDENTIALITY NOTICE*** This e-mail is the property of The Methodist Hospital and/or its relevant affiliates and may contain confidential and privileged material for the sole use of the intended recipient(s). Any review, use, distribution or disclosure by others is strictly prohibited. If you are not the intended recipient (or authorized to receive for the recipient), please contact the sender and delete all copies of the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dcojita <@t> tampabay.rr.com Thu May 22 20:15:06 2008 From: dcojita <@t> tampabay.rr.com (dcojita@tampabay.rr.com) Date: Thu May 22 20:15:20 2008 Subject: [Histonet] Hematoxylin shortage In-Reply-To: <303463.82470.qm@web58611.mail.re3.yahoo.com> Message-ID: This is fact. The hematoxylin trees were all wiped out due to past hurricanes. The shortage is real. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of KELLY BOYD Sent: Thursday, May 22, 2008 9:51 AM To: histonet Subject: [Histonet] Hematoxylin shortage Can someone please tell me what is up with all the talk about a hematoxylin shortage? There is even a vendor telling their customers they need to stock up. Is this fact or rumor? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 Tele (252)-830-6866 Cell (252)-943-9527 Fax (252)-830-0032 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rhbrown <@t> histocs.com Thu May 22 20:54:15 2008 From: rhbrown <@t> histocs.com (histocs) Date: Thu May 22 20:54:22 2008 Subject: [Histonet] looking for a service manual for the leica TP1050 Message-ID: <000901c8bc77$e7f3bde0$6500a8c0@LHBLION> Would anyone have a service manual for the TP1050. I would be happy to pay for a copy or? thanks LeRoy Brown HT(ASCP)HTL HCS Everson, WA 98247 From mohs76009 <@t> yahoo.com Thu May 22 21:16:37 2008 From: mohs76009 <@t> yahoo.com (Matt Bancroft) Date: Thu May 22 21:16:40 2008 Subject: [Histonet] Microtome blades jammed in dispenser box In-Reply-To: <516735.64474.qm@web50109.mail.re2.yahoo.com> Message-ID: <611570.86726.qm@web63410.mail.re1.yahoo.com> I recently switched to the thermo blades, I think that they last longer and have better sections. I have not had any issues with these blades Paula Pierce wrote: I am having the same problem. The tab that grabs the hole in the blades to push them forward tends to wear more than in the past and does not grab after a few have been dispensed. I have taken a very fine pair of forceps and helped the blade along and when that no loner works because the blade does not move at all I took the box apart. Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 631 N. Broadway Ave. Moore, OK 73160 405-570-6679 cell 405-759-3953 lab contact@excaliburpathology.com www.excaliburpathology.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Fri May 23 04:56:36 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri May 23 05:28:22 2008 Subject: [Histonet] Blades & Hematoxylin In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E6323@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B017E6323@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A7F23A2F@LTA3VS011.ees.hhs.gov> I wanted to give the SelecTech system a try and I even submitted slides to Surgipath who said they would stain them and return them for our review. After weeks passed I contacted Surgipath and was told they would be coming soon. After a few more months I told them to forget it. I was very disappointed that they were not responsive. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, May 22, 2008 1:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blades & Hematoxylin 1. Blades: I use the Sakura/Feathers and I just give the container a good "whack" (on the bottom) on the counter before I dispense the first blade. They have no choice but to be compliant after that. even think about our humidity level in NM at about 10%... 2. Hematoxylin: I have been reluctant because of my "position" as president of the New Mexico Society for Histology to endorse products in this venue, but the hematoxylin issue makes it necessary for me to break my rule. I recently changed to the Surgipath "SelecTech" system and I have to report rave reviews by my pathologists. The hematoxylin is good for TWENTY-FIVE HUNDRED slides - the last slides being at least as good as the first - and I've had no difficulty in getting a supply. Shelf life is 2 years. Stain quality is superb. Cost is extremely reasonable for the quality. My autostainer results had been dramatically variable until I made the change to SelecTech and although I had tried it as an economic move, it has proved to be that and more. The SelecTech system gives me beautiful results. Bosses are happy as clams with the stain now! 3. How happy IS a clam??? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jennifer.l.hofecker <@t> Vanderbilt.Edu Fri May 23 09:02:11 2008 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Fri May 23 09:02:16 2008 Subject: [Histonet] TN meeting Vendor Deadline Approaching! Message-ID: <898D946569A27444B65667A49C0740520175B4F9@mailbe06.mc.vanderbilt.edu> Happy Friday to everyone! Just a brief reminder that the vendor registration deadline for the Tennessee Society for Histotechnology Annual Meeting (June 19-21 in Townsend, TN) is quickly approaching. If you have not yet registered and are interested in exhibiting at our meeting, please contact me ASAP. All registration materials and booth fees are due to me by May 30th. If you need exhibiting information, I'll be glad to pass it along. For everyone else, it's not too late to register to attend the meeting. There are still hotel rooms available and a great variety of educational offerings. Please visit the NSH website for complete program information or feel free to contact me with questions. Have a great (hopefully long) weekend! Jennifer L. Hofecker HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph 615.343.0083 fax 615.343.7089 NSH Quality Control Committee Chairperson TSH Secretary TSH 2008 Exhibit Coordinator From michelle.schwab-macdonald <@t> novartis.com Fri May 23 09:13:03 2008 From: michelle.schwab-macdonald <@t> novartis.com (michelle.schwab-macdonald@novartis.com) Date: Fri May 23 09:10:12 2008 Subject: [Histonet] 4F1G (4% Formaldehyde & 1% Glutaraldehyde in 0.1 M PB, pH 7.4) fixative and immuno Message-ID: I am looking for anyone who might have experience with 4F1G (4% Formaldehyde &1% Gluteraldehyde in 0.1M PBS, pH 7.4) and immunohistochemistry staining. We are interested in fixing tissue in this and then making both EM and paraffin blocks from the same sample. I was interested in getting opinions on how different immuno markers, normally run on FFPE tissue, might be affected by this fixation change. I realize that it is probably marker dependent and that fixation is a huge factor in immuno, but I was just interested in some opinions or any markers that you might have tried this fixative with that will work. Thanks in Advance!! :-) Michelle Schwab-MacDonald HT(ASCP) Novartis, Boston, MA From HornHV <@t> archildrens.org Fri May 23 09:16:22 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri May 23 09:16:23 2008 Subject: [Histonet] Blades & Hematoxylin In-Reply-To: <1CE1847DFEA0A647B1CCDE4108EA60A7F23A2F@LTA3VS011.ees.hhs.gov> References: <4D14F0FC9316DD41972D5F03C070908B017E6323@nmdamailsvr.nmda.ad.nmsu.edu> <1CE1847DFEA0A647B1CCDE4108EA60A7F23A2F@LTA3VS011.ees.hhs.gov> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82C7A@EMAIL.archildrens.org> Jeanine I am sorry you had this experience. My Surgipath rep came to my lab and worked with us as we stained slides with the new stains to try them out. They are excellent. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Friday, May 23, 2008 4:57 AM To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blades & Hematoxylin I wanted to give the SelecTech system a try and I even submitted slides to Surgipath who said they would stain them and return them for our review. After weeks passed I contacted Surgipath and was told they would be coming soon. After a few more months I told them to forget it. I was very disappointed that they were not responsive. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, May 22, 2008 1:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blades & Hematoxylin 1. Blades: I use the Sakura/Feathers and I just give the container a good "whack" (on the bottom) on the counter before I dispense the first blade. They have no choice but to be compliant after that. even think about our humidity level in NM at about 10%... 2. Hematoxylin: I have been reluctant because of my "position" as president of the New Mexico Society for Histology to endorse products in this venue, but the hematoxylin issue makes it necessary for me to break my rule. I recently changed to the Surgipath "SelecTech" system and I have to report rave reviews by my pathologists. The hematoxylin is good for TWENTY-FIVE HUNDRED slides - the last slides being at least as good as the first - and I've had no difficulty in getting a supply. Shelf life is 2 years. Stain quality is superb. Cost is extremely reasonable for the quality. My autostainer results had been dramatically variable until I made the change to SelecTech and although I had tried it as an economic move, it has proved to be that and more. The SelecTech system gives me beautiful results. Bosses are happy as clams with the stain now! 3. How happy IS a clam??? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From rjbuesa <@t> yahoo.com Fri May 23 09:27:11 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri May 23 09:27:15 2008 Subject: [Histonet] 4F1G (4% Formaldehyde & 1% Glutaraldehyde in 0.1 M PB, pH 7.4) fixative and immuno In-Reply-To: Message-ID: <486224.7212.qm@web65703.mail.ac4.yahoo.com> Generally speaking you cannot get a good preservation/fixation for TEM if the block is also processed for paraffin infiltration. The reason being the thickness/size of the slice of tissue that HAS to be of 1-2 cubic mm to assure a good preservation for TEM. Other than that you just will probably have to make a stronger HIER before the IHC. I would treat one slice of tissue for FFPE and another for TEM. Ren? J. michelle.schwab-macdonald@novartis.com wrote: I am looking for anyone who might have experience with 4F1G (4% Formaldehyde &1% Gluteraldehyde in 0.1M PBS, pH 7.4) and immunohistochemistry staining. We are interested in fixing tissue in this and then making both EM and paraffin blocks from the same sample. I was interested in getting opinions on how different immuno markers, normally run on FFPE tissue, might be affected by this fixation change. I realize that it is probably marker dependent and that fixation is a huge factor in immuno, but I was just interested in some opinions or any markers that you might have tried this fixative with that will work. Thanks in Advance!! :-) Michelle Schwab-MacDonald HT(ASCP) Novartis, Boston, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lesley.bechtold <@t> jax.org Fri May 23 09:35:56 2008 From: lesley.bechtold <@t> jax.org (Lesley Bechtold) Date: Fri May 23 09:37:46 2008 Subject: [Histonet] Histology Opening In Bar Harbor, Maine Message-ID: <20080523103556689.00000001892@spikey> There is a fulltime position in the Histology Service of The Jackson Laboratory as a Histotechnologist II/III. Responsibilities include conduct of standard histological protocols including embedding, sectioning and staining as well as routine laboratory maintenance and administrative tasks. Minimum qualifications include Associate's degree in a biological science and HT(ASCP) certification plus 3-5 years of experience in histology OR a Bachelor's degree in a biological field and a minimum 3 years of experience in histology. Experience in murine histology and specialized techniques such as serial sectioning, immunohistochemistry, plastic embedding and plastic sectioning is helpful. The incumbent will have the opportunity to further their skills and knowledge. Required computer skills include email, internet, word processing, spreadsheets and familiarity with databases. Effective written and verbal communication skills are essential. Successful applicants will demonstrate good interpersonal skills and must have the ability and willingness to function effectively in a team environment. The Jackson Laboratory is one of the world's foremost centers for mammalian genetics research. Located in Bar Harbor, Maine, the lab is adjacent to Acadia National Park. Mountains, ocean, forests, lakes, and trails are all within walking distance. If you are looking for a more natural environment, this could be the opportunity you've been searching for. Interested individuals should apply on-line on the internet at www.jax.org, click on Careers at the top of the page. On Current Opportunities, click on "Bar Harbor", type in "Histology" for a keyword and click on "search". This will go directly to Job Listing 00001127. Please submit cover letter and resume online as one document. Lesley S. Bechtold Senior Manager, Histopathology & Microscopy Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 From rfields <@t> gidocs.net Fri May 23 09:49:18 2008 From: rfields <@t> gidocs.net (Rosa Fields) Date: Fri May 23 09:49:25 2008 Subject: [Histonet] Blades & Hematoxylin In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82C7A@EMAIL.archildrens.org> References: <4D14F0FC9316DD41972D5F03C070908B017E6323@nmdamailsvr.nmda.ad.nmsu.edu><1CE1847DFEA0A647B1CCDE4108EA60A7F23A2F@LTA3VS011.ees.hhs.gov> <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82C7A@EMAIL.archildrens.org> Message-ID: <2F2611250DCD6549AA3D96CE8AF1F0180109C3B6@giexchange.gidocs.net> Our Surgipath rep came to our lab also, the stain is excellent, I am very happy with it also. Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Friday, May 23, 2008 9:16 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blades & Hematoxylin Jeanine I am sorry you had this experience. My Surgipath rep came to my lab and worked with us as we stained slides with the new stains to try them out. They are excellent. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Friday, May 23, 2008 4:57 AM To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blades & Hematoxylin I wanted to give the SelecTech system a try and I even submitted slides to Surgipath who said they would stain them and return them for our review. After weeks passed I contacted Surgipath and was told they would be coming soon. After a few more months I told them to forget it. I was very disappointed that they were not responsive. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, May 22, 2008 1:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blades & Hematoxylin 1. Blades: I use the Sakura/Feathers and I just give the container a good "whack" (on the bottom) on the counter before I dispense the first blade. They have no choice but to be compliant after that. even think about our humidity level in NM at about 10%... 2. Hematoxylin: I have been reluctant because of my "position" as president of the New Mexico Society for Histology to endorse products in this venue, but the hematoxylin issue makes it necessary for me to break my rule. I recently changed to the Surgipath "SelecTech" system and I have to report rave reviews by my pathologists. The hematoxylin is good for TWENTY-FIVE HUNDRED slides - the last slides being at least as good as the first - and I've had no difficulty in getting a supply. Shelf life is 2 years. Stain quality is superb. Cost is extremely reasonable for the quality. My autostainer results had been dramatically variable until I made the change to SelecTech and although I had tried it as an economic move, it has proved to be that and more. The SelecTech system gives me beautiful results. Bosses are happy as clams with the stain now! 3. How happy IS a clam??? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kelly_colpitts <@t> hotmail.com Fri May 23 09:52:39 2008 From: kelly_colpitts <@t> hotmail.com (Kelly Colpitts) Date: Fri May 23 09:52:44 2008 Subject: [Histonet] Mixing Monoclonal and Polyclonal Negative Reagents Message-ID: To Whom It May Concern,I had posted this question earlier in the week but I realize that it was difficult to read so here it is again. Recently our lab was told that we would combine our pre-made Monoclonal Negative Antibody and our pre-made Polyclonal Negative Antibody in the same dispenser to run as the negative reagent on all cases. Would it be ok to mix a Mouse Monoclonal and a Rabbit Polyclonal or is the difference in species going to cause a problem? Thanks for your help! _________________________________________________________________ Change the world with e-mail. Join the i?m Initiative from Microsoft. http://im.live.com/Messenger/IM/Join/Default.aspx?source=EML_WL_ChangeWorld From SharonC <@t> celligent.net Fri May 23 10:14:31 2008 From: SharonC <@t> celligent.net (Sharon Campbell) Date: Fri May 23 10:11:34 2008 Subject: [Histonet] Gi biopsies and polyps Message-ID: Hello Histoworld, My lab is going to be doing many GI biopsies (new clients). Does anyone have a good short processing schedule for GI biopsies? Also, can GI polyps be on the same processing schedule? Thank you in advance, Sharon Campbell From gvdobbin <@t> ihis.org Fri May 23 11:18:03 2008 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Fri May 23 11:18:26 2008 Subject: [Histonet] Blades & Hematoxylin Message-ID: My experience with sectTech reagents is identical to that described by Jeanine (below). Big time saver when I am not troubleshooting H&E quality every other week!! Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 There is some merit in doing the right thing rather badly, but absolutely none in doing the wrong thing excellently! >>> "Bartlett, Jeanine (CDC/CCID/NCZVED)" 5/23/2008 6:56:36 AM >>> I wanted to give the SelecTech system a try and I even submitted slides to Surgipath who said they would stain them and return them for our review. After weeks passed I contacted Surgipath and was told they would be coming soon. After a few more months I told them to forget it. I was very disappointed that they were not responsive. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, May 22, 2008 1:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blades & Hematoxylin 1. Blades: I use the Sakura/Feathers and I just give the container a good "whack" (on the bottom) on the counter before I dispense the first blade. They have no choice but to be compliant after that. even think about our humidity level in NM at about 10%... 2. Hematoxylin: I have been reluctant because of my "position" as president of the New Mexico Society for Histology to endorse products in this venue, but the hematoxylin issue makes it necessary for me to break my rule. I recently changed to the Surgipath "SelecTech" system and I have to report rave reviews by my pathologists. The hematoxylin is good for TWENTY-FIVE HUNDRED slides - the last slides being at least as good as the first - and I've had no difficulty in getting a supply. Shelf life is 2 years. Stain quality is superb. Cost is extremely reasonable for the quality. My autostainer results had been dramatically variable until I made the change to SelecTech and although I had tried it as an economic move, it has proved to be that and more. The SelecTech system gives me beautiful results. Bosses are happy as clams with the stain now! 3. How happy IS a clam??? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- From gvdobbin <@t> ihis.org Fri May 23 11:20:27 2008 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Fri May 23 11:20:47 2008 Subject: [Histonet] Blades & Hematox Clarification Message-ID: I should have said my experience was the same as Sally's (ie very positive). Cheers. Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 There is some merit in doing the right thing rather badly, but absolutely none in doing the wrong thing excellently! >>> "Bartlett, Jeanine (CDC/CCID/NCZVED)" 5/23/2008 6:56:36 AM >>> I wanted to give the SelecTech system a try and I even submitted slides to Surgipath who said they would stain them and return them for our review. After weeks passed I contacted Surgipath and was told they would be coming soon. After a few more months I told them to forget it. I was very disappointed that they were not responsive. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, May 22, 2008 1:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blades & Hematoxylin 1. Blades: I use the Sakura/Feathers and I just give the container a good "whack" (on the bottom) on the counter before I dispense the first blade. They have no choice but to be compliant after that. even think about our humidity level in NM at about 10%... 2. Hematoxylin: I have been reluctant because of my "position" as president of the New Mexico Society for Histology to endorse products in this venue, but the hematoxylin issue makes it necessary for me to break my rule. I recently changed to the Surgipath "SelecTech" system and I have to report rave reviews by my pathologists. The hematoxylin is good for TWENTY-FIVE HUNDRED slides - the last slides being at least as good as the first - and I've had no difficulty in getting a supply. Shelf life is 2 years. Stain quality is superb. Cost is extremely reasonable for the quality. My autostainer results had been dramatically variable until I made the change to SelecTech and although I had tried it as an economic move, it has proved to be that and more. The SelecTech system gives me beautiful results. Bosses are happy as clams with the stain now! 3. How happy IS a clam??? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- From lblazek <@t> digestivespecialists.com Fri May 23 11:36:59 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri May 23 11:31:36 2008 Subject: [Histonet] Blades & Hematox Clarification In-Reply-To: References: Message-ID: <1F937FB30BDB7C4A9F39F83FEA8D379F9F5501@bruexchange1.digestivespecialists.com> Mine experience with SelecTech has also been great. They are a wonderful stain and the rep helped us get all of the protocols set up. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Greg Dobbin Sent: Friday, May 23, 2008 12:20 PM To: jqb7@cdc.gov; histonet@lists.utsouthwestern.edu; sbreeden@nmda.nmsu.edu Subject: RE: [Histonet] Blades & Hematox Clarification I should have said my experience was the same as Sally's (ie very positive). Cheers. Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 There is some merit in doing the right thing rather badly, but absolutely none in doing the wrong thing excellently! >>> "Bartlett, Jeanine (CDC/CCID/NCZVED)" 5/23/2008 6:56:36 AM >>> I wanted to give the SelecTech system a try and I even submitted slides to Surgipath who said they would stain them and return them for our review. After weeks passed I contacted Surgipath and was told they would be coming soon. After a few more months I told them to forget it. I was very disappointed that they were not responsive. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, May 22, 2008 1:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blades & Hematoxylin 1. Blades: I use the Sakura/Feathers and I just give the container a good "whack" (on the bottom) on the counter before I dispense the first blade. They have no choice but to be compliant after that. even think about our humidity level in NM at about 10%... 2. Hematoxylin: I have been reluctant because of my "position" as president of the New Mexico Society for Histology to endorse products in this venue, but the hematoxylin issue makes it necessary for me to break my rule. I recently changed to the Surgipath "SelecTech" system and I have to report rave reviews by my pathologists. The hematoxylin is good for TWENTY-FIVE HUNDRED slides - the last slides being at least as good as the first - and I've had no difficulty in getting a supply. Shelf life is 2 years. Stain quality is superb. Cost is extremely reasonable for the quality. My autostainer results had been dramatically variable until I made the change to SelecTech and although I had tried it as an economic move, it has proved to be that and more. The SelecTech system gives me beautiful results. Bosses are happy as clams with the stain now! 3. How happy IS a clam??? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Fri May 23 12:44:44 2008 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri May 23 12:44:53 2008 Subject: [Histonet] OT: Clams & Hematoxylin Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E6332@nmdamailsvr.nmda.ad.nmsu.edu> Alas, still no answer to my inquiry "How happy is a clam?". Mayhap a 3-day weekend will put me back on a serious track. NOT! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From Shirley_PHUA <@t> hsa.gov.sg Fri May 23 13:03:23 2008 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Fri May 23 13:07:31 2008 Subject: [Histonet] Shirley Phua is out-of-office ... Message-ID: I will be out of the office from 23-05-2008 to 24-05-2008. I'll be away on 23 May 2008 afternoon. Pathologists: I will process your requests when I return. If urgent, please forward your email to Henry_Kyaw@hsa.gov.sg From BMolinari <@t> heart.thi.tmc.edu Fri May 23 13:21:12 2008 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri May 23 13:21:21 2008 Subject: [Histonet] OT: Clams & Hematoxylin In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E6332@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: I googled the phrase and it originated in the US in a poem called "The Blind Man and the Elephant"by John G. Saxe where he used the phrase in "Sonnett to a Clam". I know I am happy as a clam when I am feasting on a bucket of steamers! Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Patholgy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, May 23, 2008 12:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OT: Clams & Hematox Alas, still no answer to my inquiry "How happy is a clam?". Mayhap a 3-day weekend will put me back on a serious track. NOT! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Fri May 23 13:33:57 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri May 23 13:28:39 2008 Subject: [Histonet] OT: Clams & Hematoxylin In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E6332@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B017E6332@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <1F937FB30BDB7C4A9F39F83FEA8D379F9F5504@bruexchange1.digestivespecialists.com> http://www.happyclambarandgrille.com/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, May 23, 2008 1:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OT: Clams & Hematoxylin Alas, still no answer to my inquiry "How happy is a clam?". Mayhap a 3-day weekend will put me back on a serious track. NOT! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Weber2 <@t> va.gov Fri May 23 13:58:37 2008 From: Susan.Weber2 <@t> va.gov (Weber, Susan (VHACLE)) Date: Fri May 23 13:58:45 2008 Subject: [Histonet] OT: Clams & Hematoxylin In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E6332@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B017E6332@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <16C83872A53F4346AA9C3A18E3A3AAB903F76DBE@VHAV10MSGA1.v10.med.va.gov> I guess the answer to your query about the happiness of clams depends if it is "invited" to the clambake, or the "guest of honor"! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, May 23, 2008 1:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OT: Clams & Hematoxylin Alas, still no answer to my inquiry "How happy is a clam?". Mayhap a 3-day weekend will put me back on a serious track. NOT! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HDOWNS <@t> PARTNERS.ORG Fri May 23 14:08:44 2008 From: HDOWNS <@t> PARTNERS.ORG (Downs, Heather M.) Date: Fri May 23 14:08:57 2008 Subject: [Histonet] Skin biopsies In-Reply-To: References: Message-ID: Heidi, I think it will depend on exactly what you are staining for. We routinely stain 3mm punch bx by fixing in Zamboni's for a few days, followed by cutting on a sliding microtome w/ dry ice @ 50 microns, and then do free floating staining for small nerve fibers. Our IHC's will only work with certain fixatives, PLP or Zamboni's Heather -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, May 23, 2008 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 54, Issue 37 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. (no subject) (Jennifer Johnson) 2. RE: (no subject) (Connolly, Brett M) 3. RE: (no subject) (Pamela Marcum) 4. Validation (Sharon.Davis-Devine) 5. Skin biopsies (Heidi Miers) 6. Hematoxylin shortage (anitaibsc@aol.com) 7. Re: Leica Peloris (Joe Nocito) 8. RE: Hematoxylin shortage (dcojita@tampabay.rr.com) 9. looking for a service manual for the leica TP1050 (histocs) 10. Re: Microtome blades jammed in dispenser box (Matt Bancroft) 11. RE: Blades & Hematoxylin (Bartlett, Jeanine (CDC/CCID/NCZVED)) 12. TN meeting Vendor Deadline Approaching! (Hofecker, Jennifer L) 13. 4F1G (4% Formaldehyde & 1% Glutaraldehyde in 0.1 M PB, pH 7.4) fixative and immuno (michelle.schwab-macdonald@novartis.com) 14. RE: Blades & Hematoxylin (Horn, Hazel V) 15. Re: 4F1G (4% Formaldehyde & 1% Glutaraldehyde in 0.1 M PB, pH 7.4) fixative and immuno (Rene J Buesa) 16. Histology Opening In Bar Harbor, Maine (Lesley Bechtold) 17. RE: Blades & Hematoxylin (Rosa Fields) 18. Mixing Monoclonal and Polyclonal Negative Reagents (Kelly Colpitts) 19. Gi biopsies and polyps (Sharon Campbell) 20. RE: Blades & Hematoxylin (Greg Dobbin) 21. RE: Blades & Hematox Clarification (Greg Dobbin) 22. RE: Blades & Hematox Clarification (Blazek, Linda) ---------------------------------------------------------------------- Message: 1 Date: Thu, 22 May 2008 16:43:34 -0400 From: Jennifer Johnson Subject: [Histonet] (no subject) To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="Windows-1252" Dear Netters, Last year I was planning a vacation to Albuquerque, NM and Sara Breeden gave me the most excellent list of things to do! I figured I would try it again. Next Wednesday I will be leaving for a two week vacation to Fiji, New Zealand, Australia, and Hawaii. Any suggestions from you locals? Please reply off list so as not to clog up the histonet. Thanks, Jennifer Johnson, HTL (ASCP) _________________________________________________________________ Change the world with e-mail. Join the i'm Initiative from Microsoft. http://im.live.com/Messenger/IM/Join/Default.aspx?source=EML_WL_ChangeWorld ------------------------------ Message: 2 Date: Thu, 22 May 2008 16:47:46 -0400 From: "Connolly, Brett M" Subject: RE: [Histonet] (no subject) To: "Jennifer Johnson" , Message-ID: <63EA0607835FBA4689CEA9EA8B482692011BAC3F@usctmx1141.merck.com> Content-Type: text/plain; charset="us-ascii" I suggest you take the rest of us with you !! Brett Brett M. Connolly, Ph.D. Research Fellow MRL, Imaging Research Merck & Co., Inc. WP-44K PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Johnson Sent: Thursday, May 22, 2008 4:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Dear Netters, Last year I was planning a vacation to Albuquerque, NM and Sara Breeden gave me the most excellent list of things to do! I figured I would try it again. Next Wednesday I will be leaving for a two week vacation to Fiji, New Zealand, Australia, and Hawaii. Any suggestions from you locals? Please reply off list so as not to clog up the histonet. Thanks, Jennifer Johnson, HTL (ASCP) _________________________________________________________________ Change the world with e-mail. Join the i'm Initiative from Microsoft. http://im.live.com/Messenger/IM/Join/Default.aspx?source=EML_WL_ChangeWo rld_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 3 Date: Thu, 22 May 2008 17:05:25 -0400 From: Pamela Marcum Subject: RE: [Histonet] (no subject) To: "Connolly, Brett M" , "Jennifer Johnson" , Message-ID: <6.2.5.6.2.20080522170454.01cc51a0@vet.upenn.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Absolutely Great Idea, Brett. She pays right?? Pam Marcum At 04:47 PM 5/22/2008, Connolly, Brett M wrote: >I suggest you take the rest of us with you !! > >Brett > >Brett M. Connolly, Ph.D. >Research Fellow >MRL, Imaging Research >Merck & Co., Inc. >WP-44K >PO Box 4 >West Point, PA 19486 >PH 215-652-2501 >fax. 215-993-6803 >e-mail. brett_connolly@merck.com > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer >Johnson >Sent: Thursday, May 22, 2008 4:44 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] (no subject) > > >Dear Netters, > >Last year I was planning a vacation to Albuquerque, NM and Sara Breeden >gave me the most excellent list of things to do! I figured I would try >it again. Next Wednesday I will be leaving for a two week vacation to >Fiji, New Zealand, Australia, and Hawaii. Any suggestions from you >locals? Please reply off list so as not to clog up the histonet. >Thanks, Jennifer Johnson, HTL (ASCP) >_________________________________________________________________ >Change the world with e-mail. Join the i'm Initiative from Microsoft. >http://im.live.com/Messenger/IM/Join/Default.aspx?source=EML_WL_ChangeWo >rld_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >Notice: This e-mail message, together with any attachments, >contains information of Merck & Co., Inc. (One Merck Drive, >Whitehouse Station, New Jersey, USA 08889), and/or its affiliates >(which may be known outside the United States as Merck Frosst, Merck >Sharp & Dohme or MSD and in Japan, as Banyu - direct contact >information for affiliates is available at >http://www.merck.com/contact/contacts.html) that may be >confidential, proprietary copyrighted and/or legally privileged. It >is intended solely for the use of the individual or entity named on >this message. If you are not the intended recipient, and have >received this message in error, please notify us immediately by >reply e-mail and then delete it from your system. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu ------------------------------ Message: 4 Date: Thu, 22 May 2008 16:13:37 -0500 From: "Sharon.Davis-Devine" Subject: [Histonet] Validation To: Message-ID: <44780C571F28624DBB446DE55C4D733A021E09A6@EXCHANGEBE1.carle.com> Content-Type: text/plain; charset="us-ascii" We are currently in the process of switching from a Dako immuno stainer to the Ventana system. We need to do a validation for the new system but need ideas from everyone out there who has done a validation to give us some pointers on how to accomplish this. Any information is greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com ------------------------------ Message: 5 Date: Thu, 22 May 2008 15:27:01 -0700 From: Heidi Miers Subject: [Histonet] Skin biopsies To: histonet@lists.utsouthwestern.edu Message-ID: <4835F335.2020509@nau.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Does anyone have any advice on tissue processing times for 0.5 to 1 mm thick skin biopsies for IHC? Thanks to all in advance, Heidi ------------------------------ Message: 6 Date: Thu, 22 May 2008 18:51:10 -0400 From: anitaibsc@aol.com Subject: [Histonet] Hematoxylin shortage To: histonet@lists.utsouthwestern.edu Message-ID: <8CA8A757697861D-8C4-8D3@webmail-nd19.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Hello Netteres, The information?I provided was to assist with a specific product and was not intended as a mass, commercial advertisement. IBSC makes variety of IHC products not just Hematoxylin. Anita ------------------------------ Message: 7 Date: Thu, 22 May 2008 18:51:41 -0500 From: "Joe Nocito" Subject: Re: [Histonet] Leica Peloris To: "Laurie Colbert" , "Mike Pence" , "Salcedo, Rudy" , Message-ID: <00c001c8bc66$c8d0c3b0$0302a8c0@yourxhtr8hvc4p> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original we just purchased two, with a third on the way. We took off the xylene and the tissue has processed better. We had to monkey around with our fatty specimens a little. We run a 4 hour and an 8 hour xylene-free program. Next week, we try the 1.5 hour xylene free on real specimens. We are trying to process throughout the day. So far, I like them. But, need to see the response time if a repair needs to be made. This company has not had a good track record in the past. JTT ----- Original Message ----- From: "Laurie Colbert" To: "Mike Pence" ; "Salcedo, Rudy" ; Sent: Thursday, May 22, 2008 1:16 PM Subject: RE: [Histonet] Leica Peloris Do you use xylene on the Peloris? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Thursday, May 22, 2008 9:19 AM To: Salcedo, Rudy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica Peloris I just purchased a Peloris about 3 months ago. Love it. I can process all thru the day and it has made a huge impact on turn around times. We cut fewer blocks thru out the day and I think it processes fatty specimens better. I have a few small issues, but I have been very happy with the processor. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Salcedo, Rudy Sent: Thursday, May 22, 2008 10:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Peloris Histonet, Any labs out there using the Leica Peloris Processor, if so please send me some feed back on you experience with the Peloris. Thanks in advance. Rudy Salcedo Houston, Texas NOTICE: Our e-mail address at Methodist has changed to tmhs.org Methodist. Leading Medicine. Ranked No. 10 on FORTUNE magazine's list of the "100 Best Companies to Work For" in 2008 Named by U.S. News & World Report as one of "America's Best Hospitals" Designated as a Magnet hospital for excellence in nursing ***CONFIDENTIALITY NOTICE*** This e-mail is the property of The Methodist Hospital and/or its relevant affiliates and may contain confidential and privileged material for the sole use of the intended recipient(s). Any review, use, distribution or disclosure by others is strictly prohibited. If you are not the intended recipient (or authorized to receive for the recipient), please contact the sender and delete all copies of the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Thu, 22 May 2008 21:15:06 -0400 From: Subject: RE: [Histonet] Hematoxylin shortage To: "'KELLY BOYD'" , "'histonet'" Message-ID: Content-Type: text/plain; charset="US-ASCII" This is fact. The hematoxylin trees were all wiped out due to past hurricanes. The shortage is real. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of KELLY BOYD Sent: Thursday, May 22, 2008 9:51 AM To: histonet Subject: [Histonet] Hematoxylin shortage Can someone please tell me what is up with all the talk about a hematoxylin shortage? There is even a vendor telling their customers they need to stock up. Is this fact or rumor? Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 Tele (252)-830-6866 Cell (252)-943-9527 Fax (252)-830-0032 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Thu, 22 May 2008 18:54:15 -0700 From: "histocs" Subject: [Histonet] looking for a service manual for the leica TP1050 To: "Histonet" Message-ID: <000901c8bc77$e7f3bde0$6500a8c0@LHBLION> Content-Type: text/plain; charset="Windows-1252" Would anyone have a service manual for the TP1050. I would be happy to pay for a copy or? thanks LeRoy Brown HT(ASCP)HTL HCS Everson, WA 98247 ------------------------------ Message: 10 Date: Thu, 22 May 2008 19:16:37 -0700 (PDT) From: Matt Bancroft Subject: Re: [Histonet] Microtome blades jammed in dispenser box To: Paula Pierce , Histonet Message-ID: <611570.86726.qm@web63410.mail.re1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I recently switched to the thermo blades, I think that they last longer and have better sections. I have not had any issues with these blades Paula Pierce wrote: I am having the same problem. The tab that grabs the hole in the blades to push them forward tends to wear more than in the past and does not grab after a few have been dispensed. I have taken a very fine pair of forceps and helped the blade along and when that no loner works because the blade does not move at all I took the box apart. Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 631 N. Broadway Ave. Moore, OK 73160 405-570-6679 cell 405-759-3953 lab contact@excaliburpathology.com www.excaliburpathology.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Fri, 23 May 2008 05:56:36 -0400 From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" Subject: RE: [Histonet] Blades & Hematoxylin To: "Breeden, Sara" , histonet@lists.utsouthwestern.edu Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A7F23A2F@LTA3VS011.ees.hhs.gov> Content-Type: text/plain; charset=us-ascii I wanted to give the SelecTech system a try and I even submitted slides to Surgipath who said they would stain them and return them for our review. After weeks passed I contacted Surgipath and was told they would be coming soon. After a few more months I told them to forget it. I was very disappointed that they were not responsive. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, May 22, 2008 1:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blades & Hematoxylin 1. Blades: I use the Sakura/Feathers and I just give the container a good "whack" (on the bottom) on the counter before I dispense the first blade. They have no choice but to be compliant after that. even think about our humidity level in NM at about 10%... 2. Hematoxylin: I have been reluctant because of my "position" as president of the New Mexico Society for Histology to endorse products in this venue, but the hematoxylin issue makes it necessary for me to break my rule. I recently changed to the Surgipath "SelecTech" system and I have to report rave reviews by my pathologists. The hematoxylin is good for TWENTY-FIVE HUNDRED slides - the last slides being at least as good as the first - and I've had no difficulty in getting a supply. Shelf life is 2 years. Stain quality is superb. Cost is extremely reasonable for the quality. My autostainer results had been dramatically variable until I made the change to SelecTech and although I had tried it as an economic move, it has proved to be that and more. The SelecTech system gives me beautiful results. Bosses are happy as clams with the stain now! 3. How happy IS a clam??? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Fri, 23 May 2008 09:02:11 -0500 From: "Hofecker, Jennifer L" Subject: [Histonet] TN meeting Vendor Deadline Approaching! To: "histonet" Message-ID: <898D946569A27444B65667A49C0740520175B4F9@mailbe06.mc.vanderbilt.edu> Content-Type: text/plain; charset="us-ascii" Happy Friday to everyone! Just a brief reminder that the vendor registration deadline for the Tennessee Society for Histotechnology Annual Meeting (June 19-21 in Townsend, TN) is quickly approaching. If you have not yet registered and are interested in exhibiting at our meeting, please contact me ASAP. All registration materials and booth fees are due to me by May 30th. If you need exhibiting information, I'll be glad to pass it along. For everyone else, it's not too late to register to attend the meeting. There are still hotel rooms available and a great variety of educational offerings. Please visit the NSH website for complete program information or feel free to contact me with questions. Have a great (hopefully long) weekend! Jennifer L. Hofecker HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph 615.343.0083 fax 615.343.7089 NSH Quality Control Committee Chairperson TSH Secretary TSH 2008 Exhibit Coordinator ------------------------------ Message: 13 Date: Fri, 23 May 2008 10:13:03 -0400 From: michelle.schwab-macdonald@novartis.com Subject: [Histonet] 4F1G (4% Formaldehyde & 1% Glutaraldehyde in 0.1 M PB, pH 7.4) fixative and immuno To: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" I am looking for anyone who might have experience with 4F1G (4% Formaldehyde &1% Gluteraldehyde in 0.1M PBS, pH 7.4) and immunohistochemistry staining. We are interested in fixing tissue in this and then making both EM and paraffin blocks from the same sample. I was interested in getting opinions on how different immuno markers, normally run on FFPE tissue, might be affected by this fixation change. I realize that it is probably marker dependent and that fixation is a huge factor in immuno, but I was just interested in some opinions or any markers that you might have tried this fixative with that will work. Thanks in Advance!! :-) Michelle Schwab-MacDonald HT(ASCP) Novartis, Boston, MA ------------------------------ Message: 14 Date: Fri, 23 May 2008 09:16:22 -0500 From: "Horn, Hazel V" Subject: RE: [Histonet] Blades & Hematoxylin To: "Bartlett, Jeanine (CDC/CCID/NCZVED)" , "Breeden, Sara" , Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82C7A@EMAIL.archildrens.org> Content-Type: text/plain; charset="us-ascii" Jeanine I am sorry you had this experience. My Surgipath rep came to my lab and worked with us as we stained slides with the new stains to try them out. They are excellent. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Friday, May 23, 2008 4:57 AM To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blades & Hematoxylin I wanted to give the SelecTech system a try and I even submitted slides to Surgipath who said they would stain them and return them for our review. After weeks passed I contacted Surgipath and was told they would be coming soon. After a few more months I told them to forget it. I was very disappointed that they were not responsive. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, May 22, 2008 1:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blades & Hematoxylin 1. Blades: I use the Sakura/Feathers and I just give the container a good "whack" (on the bottom) on the counter before I dispense the first blade. They have no choice but to be compliant after that. even think about our humidity level in NM at about 10%... 2. Hematoxylin: I have been reluctant because of my "position" as president of the New Mexico Society for Histology to endorse products in this venue, but the hematoxylin issue makes it necessary for me to break my rule. I recently changed to the Surgipath "SelecTech" system and I have to report rave reviews by my pathologists. The hematoxylin is good for TWENTY-FIVE HUNDRED slides - the last slides being at least as good as the first - and I've had no difficulty in getting a supply. Shelf life is 2 years. Stain quality is superb. Cost is extremely reasonable for the quality. My autostainer results had been dramatically variable until I made the change to SelecTech and although I had tried it as an economic move, it has proved to be that and more. The SelecTech system gives me beautiful results. Bosses are happy as clams with the stain now! 3. How happy IS a clam??? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** ******************************************************************************** ******************************************************************************** ******************************************************************************** ******************************************************************************** ******************************************************************************** ******************************************************************************** ********************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ------------------------------ Message: 15 Date: Fri, 23 May 2008 07:27:11 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] 4F1G (4% Formaldehyde & 1% Glutaraldehyde in 0.1 M PB, pH 7.4) fixative and immuno To: michelle.schwab-macdonald@novartis.com, histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: <486224.7212.qm@web65703.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Generally speaking you cannot get a good preservation/fixation for TEM if the block is also processed for paraffin infiltration. The reason being the thickness/size of the slice of tissue that HAS to be of 1-2 cubic mm to assure a good preservation for TEM. Other than that you just will probably have to make a stronger HIER before the IHC. I would treat one slice of tissue for FFPE and another for TEM. Ren? J. michelle.schwab-macdonald@novartis.com wrote: I am looking for anyone who might have experience with 4F1G (4% Formaldehyde &1% Gluteraldehyde in 0.1M PBS, pH 7.4) and immunohistochemistry staining. We are interested in fixing tissue in this and then making both EM and paraffin blocks from the same sample. I was interested in getting opinions on how different immuno markers, normally run on FFPE tissue, might be affected by this fixation change. I realize that it is probably marker dependent and that fixation is a huge factor in immuno, but I was just interested in some opinions or any markers that you might have tried this fixative with that will work. Thanks in Advance!! :-) Michelle Schwab-MacDonald HT(ASCP) Novartis, Boston, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Fri, 23 May 2008 10:35:56 -0400 From: "Lesley Bechtold" Subject: [Histonet] Histology Opening In Bar Harbor, Maine To: "histonet@lists.utsouthwestern.edu" Message-ID: <20080523103556689.00000001892@spikey> Content-Type: text/plain; charset=us-ascii There is a fulltime position in the Histology Service of The Jackson Laboratory as a Histotechnologist II/III. Responsibilities include conduct of standard histological protocols including embedding, sectioning and staining as well as routine laboratory maintenance and administrative tasks. Minimum qualifications include Associate's degree in a biological science and HT(ASCP) certification plus 3-5 years of experience in histology OR a Bachelor's degree in a biological field and a minimum 3 years of experience in histology. Experience in murine histology and specialized techniques such as serial sectioning, immunohistochemistry, plastic embedding and plastic sectioning is helpful. The incumbent will have the opportunity to further their skills and knowledge. Required computer skills include email, internet, word processing, spreadsheets and familiarity with databases. Effective written and verbal communication skills are essential. Successful applicants will demonstrate good interpersonal skills and must have the ability and willingness to function effectively in a team environment. The Jackson Laboratory is one of the world's foremost centers for mammalian genetics research. Located in Bar Harbor, Maine, the lab is adjacent to Acadia National Park. Mountains, ocean, forests, lakes, and trails are all within walking distance. If you are looking for a more natural environment, this could be the opportunity you've been searching for. Interested individuals should apply on-line on the internet at www.jax.org, click on Careers at the top of the page. On Current Opportunities, click on "Bar Harbor", type in "Histology" for a keyword and click on "search". This will go directly to Job Listing 00001127. Please submit cover letter and resume online as one document. Lesley S. Bechtold Senior Manager, Histopathology & Microscopy Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 ------------------------------ Message: 17 Date: Fri, 23 May 2008 09:49:18 -0500 From: "Rosa Fields" Subject: RE: [Histonet] Blades & Hematoxylin To: "Horn, Hazel V" , "Bartlett, Jeanine \(CDC/CCID/NCZVED\)" , "Breeden, Sara" , Message-ID: <2F2611250DCD6549AA3D96CE8AF1F0180109C3B6@giexchange.gidocs.net> Content-Type: text/plain; charset="iso-8859-1" Our Surgipath rep came to our lab also, the stain is excellent, I am very happy with it also. Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Friday, May 23, 2008 9:16 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blades & Hematoxylin Jeanine I am sorry you had this experience. My Surgipath rep came to my lab and worked with us as we stained slides with the new stains to try them out. They are excellent. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Friday, May 23, 2008 4:57 AM To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blades & Hematoxylin I wanted to give the SelecTech system a try and I even submitted slides to Surgipath who said they would stain them and return them for our review. After weeks passed I contacted Surgipath and was told they would be coming soon. After a few more months I told them to forget it. I was very disappointed that they were not responsive. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, May 22, 2008 1:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blades & Hematoxylin 1. Blades: I use the Sakura/Feathers and I just give the container a good "whack" (on the bottom) on the counter before I dispense the first blade. They have no choice but to be compliant after that. even think about our humidity level in NM at about 10%... 2. Hematoxylin: I have been reluctant because of my "position" as president of the New Mexico Society for Histology to endorse products in this venue, but the hematoxylin issue makes it necessary for me to break my rule. I recently changed to the Surgipath "SelecTech" system and I have to report rave reviews by my pathologists. The hematoxylin is good for TWENTY-FIVE HUNDRED slides - the last slides being at least as good as the first - and I've had no difficulty in getting a supply. Shelf life is 2 years. Stain quality is superb. Cost is extremely reasonable for the quality. My autostainer results had been dramatically variable until I made the change to SelecTech and although I had tried it as an economic move, it has proved to be that and more. The SelecTech system gives me beautiful results. Bosses are happy as clams with the stain now! 3. How happy IS a clam??? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** ******************************************************************************** ******************************************************************************** ******************************************************************************** ******************************************************************************** ******************************************************************************** ******************************************************************************** ********************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Fri, 23 May 2008 10:52:39 -0400 From: Kelly Colpitts Subject: [Histonet] Mixing Monoclonal and Polyclonal Negative Reagents To: Message-ID: Content-Type: text/plain; charset="Windows-1252" To Whom It May Concern,I had posted this question earlier in the week but I realize that it was difficult to read so here it is again. Recently our lab was told that we would combine our pre-made Monoclonal Negative Antibody and our pre-made Polyclonal Negative Antibody in the same dispenser to run as the negative reagent on all cases. Would it be ok to mix a Mouse Monoclonal and a Rabbit Polyclonal or is the difference in species going to cause a problem? Thanks for your help! _________________________________________________________________ Change the world with e-mail. Join the i'm Initiative from Microsoft. http://im.live.com/Messenger/IM/Join/Default.aspx?source=EML_WL_ChangeWorld ------------------------------ Message: 19 Date: Fri, 23 May 2008 11:14:31 -0400 From: "Sharon Campbell" Subject: [Histonet] Gi biopsies and polyps To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hello Histoworld, My lab is going to be doing many GI biopsies (new clients). Does anyone have a good short processing schedule for GI biopsies? Also, can GI polyps be on the same processing schedule? Thank you in advance, Sharon Campbell ------------------------------ Message: 20 Date: Fri, 23 May 2008 13:18:03 -0300 From: "Greg Dobbin" Subject: RE: [Histonet] Blades & Hematoxylin To: ,, Message-ID: Content-Type: text/plain; charset=US-ASCII My experience with sectTech reagents is identical to that described by Jeanine (below). Big time saver when I am not troubleshooting H&E quality every other week!! Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 There is some merit in doing the right thing rather badly, but absolutely none in doing the wrong thing excellently! >>> "Bartlett, Jeanine (CDC/CCID/NCZVED)" 5/23/2008 6:56:36 AM >>> I wanted to give the SelecTech system a try and I even submitted slides to Surgipath who said they would stain them and return them for our review. After weeks passed I contacted Surgipath and was told they would be coming soon. After a few more months I told them to forget it. I was very disappointed that they were not responsive. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, May 22, 2008 1:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blades & Hematoxylin 1. Blades: I use the Sakura/Feathers and I just give the container a good "whack" (on the bottom) on the counter before I dispense the first blade. They have no choice but to be compliant after that. even think about our humidity level in NM at about 10%... 2. Hematoxylin: I have been reluctant because of my "position" as president of the New Mexico Society for Histology to endorse products in this venue, but the hematoxylin issue makes it necessary for me to break my rule. I recently changed to the Surgipath "SelecTech" system and I have to report rave reviews by my pathologists. The hematoxylin is good for TWENTY-FIVE HUNDRED slides - the last slides being at least as good as the first - and I've had no difficulty in getting a supply. Shelf life is 2 years. Stain quality is superb. Cost is extremely reasonable for the quality. My autostainer results had been dramatically variable until I made the change to SelecTech and although I had tried it as an economic move, it has proved to be that and more. The SelecTech system gives me beautiful results. Bosses are happy as clams with the stain now! 3. How happy IS a clam??? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- ------------------------------ Message: 21 Date: Fri, 23 May 2008 13:20:27 -0300 From: "Greg Dobbin" Subject: RE: [Histonet] Blades & Hematox Clarification To: ,, Message-ID: Content-Type: text/plain; charset=US-ASCII I should have said my experience was the same as Sally's (ie very positive). Cheers. Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 There is some merit in doing the right thing rather badly, but absolutely none in doing the wrong thing excellently! >>> "Bartlett, Jeanine (CDC/CCID/NCZVED)" 5/23/2008 6:56:36 AM >>> I wanted to give the SelecTech system a try and I even submitted slides to Surgipath who said they would stain them and return them for our review. After weeks passed I contacted Surgipath and was told they would be coming soon. After a few more months I told them to forget it. I was very disappointed that they were not responsive. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, May 22, 2008 1:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blades & Hematoxylin 1. Blades: I use the Sakura/Feathers and I just give the container a good "whack" (on the bottom) on the counter before I dispense the first blade. They have no choice but to be compliant after that. even think about our humidity level in NM at about 10%... 2. Hematoxylin: I have been reluctant because of my "position" as president of the New Mexico Society for Histology to endorse products in this venue, but the hematoxylin issue makes it necessary for me to break my rule. I recently changed to the Surgipath "SelecTech" system and I have to report rave reviews by my pathologists. The hematoxylin is good for TWENTY-FIVE HUNDRED slides - the last slides being at least as good as the first - and I've had no difficulty in getting a supply. Shelf life is 2 years. Stain quality is superb. Cost is extremely reasonable for the quality. My autostainer results had been dramatically variable until I made the change to SelecTech and although I had tried it as an economic move, it has proved to be that and more. The SelecTech system gives me beautiful results. Bosses are happy as clams with the stain now! 3. How happy IS a clam??? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- ------------------------------ Message: 22 Date: Fri, 23 May 2008 12:36:59 -0400 From: "Blazek, Linda" Subject: RE: [Histonet] Blades & Hematox Clarification To: "Greg Dobbin" , , , Message-ID: <1F937FB30BDB7C4A9F39F83FEA8D379F9F5501@bruexchange1.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" Mine experience with SelecTech has also been great. They are a wonderful stain and the rep helped us get all of the protocols set up. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Greg Dobbin Sent: Friday, May 23, 2008 12:20 PM To: jqb7@cdc.gov; histonet@lists.utsouthwestern.edu; sbreeden@nmda.nmsu.edu Subject: RE: [Histonet] Blades & Hematox Clarification I should have said my experience was the same as Sally's (ie very positive). Cheers. Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 There is some merit in doing the right thing rather badly, but absolutely none in doing the wrong thing excellently! >>> "Bartlett, Jeanine (CDC/CCID/NCZVED)" 5/23/2008 6:56:36 AM >>> I wanted to give the SelecTech system a try and I even submitted slides to Surgipath who said they would stain them and return them for our review. After weeks passed I contacted Surgipath and was told they would be coming soon. After a few more months I told them to forget it. I was very disappointed that they were not responsive. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, May 22, 2008 1:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blades & Hematoxylin 1. Blades: I use the Sakura/Feathers and I just give the container a good "whack" (on the bottom) on the counter before I dispense the first blade. They have no choice but to be compliant after that. even think about our humidity level in NM at about 10%... 2. Hematoxylin: I have been reluctant because of my "position" as president of the New Mexico Society for Histology to endorse products in this venue, but the hematoxylin issue makes it necessary for me to break my rule. I recently changed to the Surgipath "SelecTech" system and I have to report rave reviews by my pathologists. The hematoxylin is good for TWENTY-FIVE HUNDRED slides - the last slides being at least as good as the first - and I've had no difficulty in getting a supply. Shelf life is 2 years. Stain quality is superb. Cost is extremely reasonable for the quality. My autostainer results had been dramatically variable until I made the change to SelecTech and although I had tried it as an economic move, it has proved to be that and more. The SelecTech system gives me beautiful results. Bosses are happy as clams with the stain now! 3. How happy IS a clam??? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 54, Issue 37 **************************************** The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From pruegg <@t> ihctech.net Sun May 25 10:05:46 2008 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Sun May 25 10:05:35 2008 Subject: [Histonet] NSH bulletin (In Action) In-Reply-To: <4832EA8F.6EA2.00FD.0@vetmed.ufl.edu> Message-ID: <200805251505.m4PF5MYE082758@pro42.abac.com> Please elaborate! Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MaryAnn Dixon Sent: Tuesday, May 20, 2008 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NSH bulletin (In Action) Hi Histonetters, Has anyone read the In Action NSH bulletin lately? Just another example of the lack of recognition for the field of Histology. I am new to the field of Histology. What has happened to those employed in the NY labs? Are other states following by example? Should I get out now before I am unemployed? MaryAnn Dixon Biological Scientist Anatomic Pathology University of Florida Medical Center 352-392-2235 ext 4517 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun May 25 10:07:31 2008 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Sun May 25 10:07:18 2008 Subject: [Histonet] Sample antibodies In-Reply-To: Message-ID: <200805251507.m4PF77EK084198@pro42.abac.com> So does Novacastra now Leica and I have just recently been getting samples from Cell Marque, usually if they package in 100ul size they will give you a sample of that. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A. Sent: Tuesday, May 20, 2008 1:11 PM To: cmmathis1@bellsouth.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Sample antibodies NeoMarkers sells 0.1 ml sizes. These are marketed through the Lab Vision division of Thermo Scientific, phone #: 1(800)828-1628. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of cmmathis1@bellsouth.net Sent: Tuesday, May 20, 2008 1:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sample antibodies Are there any companies that have sample antibodies or any kind of trial size? Cathy Mathis _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From maxdad <@t> wi.rr.com Mon May 26 13:37:13 2008 From: maxdad <@t> wi.rr.com (Brad Miller) Date: Mon May 26 13:37:11 2008 Subject: [Histonet] NSH bulletin (In Action) References: <200805251505.m4PF5MYE082758@pro42.abac.com> Message-ID: <002601c8bf5f$8433e150$6701a8c0@BRAD> NYS Legislative Update - April 6, 2008 In the last few weeks there has been a lot of activity on the legislative front in NYS. As a recap, a law (Article 165) requiring licensing of all clinical laboratory personnel was passed by the NYS legislation and went into effect in September of 2006 (please visit http://www.op.nysed.gov/clp-cltlic.htm for specific details). Unfortunately, histologists did not have representation on the board that crafted the license nor did we have lobbyists working for our interests. As a result there is no language describing the scope of practice for histologists, no distinction between technician and technologist and no provision for academic training requirements (curriculum). ----- Original Message ----- From: "patsy ruegg" To: "'MaryAnn Dixon'" ; Sent: Sunday, May 25, 2008 10:05 AM Subject: RE: [Histonet] NSH bulletin (In Action) > Please elaborate! > > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. #215 > Aurora, CO 80045 > 720-859-4060 > fax 720-859-4110 > pruegg@ihctech.net > www.ihctech.net > www.ihcrg.org > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MaryAnn > Dixon > Sent: Tuesday, May 20, 2008 1:13 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] NSH bulletin (In Action) > > Hi Histonetters, > > Has anyone read the In Action NSH bulletin lately? Just another example > of > the lack of recognition for the field of Histology. I am new to the field > of Histology. What has happened to those employed in the NY labs? Are > other states following by example? Should I get out now before I am > unemployed? > > MaryAnn Dixon > Biological Scientist > Anatomic Pathology > University of Florida Medical Center > 352-392-2235 ext 4517 > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG. > Version: 7.5.524 / Virus Database: 269.24.1/1464 - Release Date: 5/24/2008 > 8:56 AM > > From AnthonyH <@t> chw.edu.au Mon May 26 18:42:41 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon May 26 18:42:47 2008 Subject: [Histonet] RE: EDTA decalcification solution- trying to contact Maxim in Russia In-Reply-To: <1932728407.20080519230920@mail.ru> Message-ID: To Maxim Peshkov from Taganrog in Russia I can't seem to be able to send emails through to you so have been unable to send you the information you asked for. So, with apologies to our fellow Histonetters, I know that you seem to be able to receive emails from Histonet so I post this there. Please contact me and I will see what we can do. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From lpwenk <@t> sbcglobal.net Mon May 26 18:46:03 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Mon May 26 18:46:16 2008 Subject: [Histonet] NSH bulletin (In Action) In-Reply-To: <002601c8bf5f$8433e150$6701a8c0@BRAD> Message-ID: <000c01c8bf8a$a8eb8ae0$2d0c2e4b@HPPav2> I'm not an expert in the NY licensure, but I'll fill in some gaps from what I've learned from Histonets and talking with NY histotechs. The law that was passed in 2006 allows for Cytotechs, MLT's and MT's. Though HT/HTL were originally written into the licensure bill when it was first drafted over a decade ago, somewhere over time, HT and HTL were dropped from the NY bill. So as of right now, any histotech currently working in NY are grandfathered in, so they can continue to work as histotechs in NY. Any new histotech coming into the field in NY, or already experienced and moving to NY, must take all the classes to be a MT or a MLT, AND pass the MT/MLT exam. ASCP and NSH and the NY histotechs and the NY pathologists societies have been working to try to get an amendment to the law, recognizing HT/HTL as a separate tech category. However, once a law has been adopted, it becomes very hard to get the legislators to agree to change it. And can be difficult to get the wording correct (both of which are what is happening now). There are other states out there, working on licensure (Michigan, where I'm from, have been working on it for over 15 years). But it's up to us histotechs to remain aware of what other lab societies within our own states are working on, so histotechs don't get left out of a law. (The Michigan draft does list histotechs (HT and HTL), as well as PA's, cytogenetic technologists, EM techs, etc. However, we have 2 histotechs in our state who try to keep track of where the draft is - which legislatures are behind it, what the lobbyists are doing. It's being pushed by the med tech society in the state, but they (med tehc society) have been working with all the Michigan lab societies. The Michigan med techs are very aware of what happened in NY, and don't want it to happen here.) So, no, don't get out of the histotechnology. We need people like yourself, who are aware of the effects of state and national laws on our profession, and who are willing to look out for our field. And who would be willing to talk with cytotechs and med techs, and remind them that there are other lab professionals. For the latest from the NSH web page, go to: http://www.nsh.org/organizations.php3?action=printContentItem&orgid=111&type ID=1203&itemID=22168 Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brad Miller Sent: Monday, May 26, 2008 2:37 PM To: patsy ruegg; 'MaryAnn Dixon'; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] NSH bulletin (In Action) NYS Legislative Update - April 6, 2008 In the last few weeks there has been a lot of activity on the legislative front in NYS. As a recap, a law (Article 165) requiring licensing of all clinical laboratory personnel was passed by the NYS legislation and went into effect in September of 2006 (please visit http://www.op.nysed.gov/clp-cltlic.htm for specific details). Unfortunately, histologists did not have representation on the board that crafted the license nor did we have lobbyists working for our interests. As a result there is no language describing the scope of practice for histologists, no distinction between technician and technologist and no provision for academic training requirements (curriculum). ----- Original Message ----- From: "patsy ruegg" To: "'MaryAnn Dixon'" ; Sent: Sunday, May 25, 2008 10:05 AM Subject: RE: [Histonet] NSH bulletin (In Action) > Please elaborate! > > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. #215 > Aurora, CO 80045 > 720-859-4060 > fax 720-859-4110 > pruegg@ihctech.net > www.ihctech.net > www.ihcrg.org > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > MaryAnn Dixon > Sent: Tuesday, May 20, 2008 1:13 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] NSH bulletin (In Action) > > Hi Histonetters, > > Has anyone read the In Action NSH bulletin lately? Just another > example of the lack of recognition for the field of Histology. I am > new to the field of Histology. What has happened to those employed in > the NY labs? Are other states following by example? Should I get out > now before I am unemployed? > > MaryAnn Dixon > Biological Scientist > Anatomic Pathology > University of Florida Medical Center > 352-392-2235 ext 4517 > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG. > Version: 7.5.524 / Virus Database: 269.24.1/1464 - Release Date: > 5/24/2008 > 8:56 AM > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From minhan.tan <@t> gmail.com Mon May 26 21:55:59 2008 From: minhan.tan <@t> gmail.com (Min-Han Tan) Date: Mon May 26 21:56:04 2008 Subject: [Histonet] Biotin free detection of goat primary antibodies Message-ID: <920cc4a70805261955y50c3d198n6d46aa42ba9c20b3@mail.gmail.com> Dear all, I would like to enquire - I am using a goat primary antibody currently, and my negative control (omitting primary antibody) is showing background staining - this is likely a result of biotin. I'd like to enquire if any one is familiar with biotin free systems that can detect goat antibodies. A search of the Dako / Envision product line does not yield any results. I am very reluctant to add an avidin-biotin blocking step to what is already an overnight incubation. Thanks! -- /min-han From annigyg <@t> gmail.com Mon May 26 23:11:15 2008 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Mon May 26 23:11:19 2008 Subject: [Histonet] IF question Message-ID: Hi Histonetters is anyone out there running IF controls - specifically for renals, skins if so what and how? please advise asap thanks Anne -- From mickie25 <@t> netzero.net Tue May 27 08:56:45 2008 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Tue May 27 08:56:48 2008 Subject: [Histonet] NSH bulletin (In Action) In-Reply-To: <000c01c8bf8a$a8eb8ae0$2d0c2e4b@HPPav2> References: <002601c8bf5f$8433e150$6701a8c0@BRAD> <000c01c8bf8a$a8eb8ae0$2d0c2e4b@HPPav2> Message-ID: Hello Peggy, Thank you for your update. There has been quite a bit of discussion about this in the American College of Mohs Surgeons, American Society of Mohs Surgery and the American Society of Mohs Histotechnology over the last year. The majority of Mohs histotechs are not registered HT's or HTL's. At this time it is just under discussion with questionnaires. I would be interested to know if there is any resource available that covers the requirements for licensure in each state? Would knowledgeable persons from each state be willing to post what they know about the requirements in the states they reside and work in? Right now, Washington State has no requirements for licensure of histotechs, Mohs or otherwise. If people will email me I will compile what I receive and post a compilation if this has not already been done. Thank you. Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Monday, May 26, 2008 4:46 PM To: 'Brad Miller'; 'patsy ruegg'; 'MaryAnn Dixon'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH bulletin (In Action) I'm not an expert in the NY licensure, but I'll fill in some gaps from what I've learned from Histonets and talking with NY histotechs. The law that was passed in 2006 allows for Cytotechs, MLT's and MT's. Though HT/HTL were originally written into the licensure bill when it was first drafted over a decade ago, somewhere over time, HT and HTL were dropped from the NY bill. So as of right now, any histotech currently working in NY are grandfathered in, so they can continue to work as histotechs in NY. Any new histotech coming into the field in NY, or already experienced and moving to NY, must take all the classes to be a MT or a MLT, AND pass the MT/MLT exam. ASCP and NSH and the NY histotechs and the NY pathologists societies have been working to try to get an amendment to the law, recognizing HT/HTL as a separate tech category. However, once a law has been adopted, it becomes very hard to get the legislators to agree to change it. And can be difficult to get the wording correct (both of which are what is happening now). There are other states out there, working on licensure (Michigan, where I'm from, have been working on it for over 15 years). But it's up to us histotechs to remain aware of what other lab societies within our own states are working on, so histotechs don't get left out of a law. (The Michigan draft does list histotechs (HT and HTL), as well as PA's, cytogenetic technologists, EM techs, etc. However, we have 2 histotechs in our state who try to keep track of where the draft is - which legislatures are behind it, what the lobbyists are doing. It's being pushed by the med tech society in the state, but they (med tehc society) have been working with all the Michigan lab societies. The Michigan med techs are very aware of what happened in NY, and don't want it to happen here.) So, no, don't get out of the histotechnology. We need people like yourself, who are aware of the effects of state and national laws on our profession, and who are willing to look out for our field. And who would be willing to talk with cytotechs and med techs, and remind them that there are other lab professionals. For the latest from the NSH web page, go to: http://www.nsh.org/organizations.php3?action=printContentItem&orgid=111&type ID=1203&itemID=22168 Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brad Miller Sent: Monday, May 26, 2008 2:37 PM To: patsy ruegg; 'MaryAnn Dixon'; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] NSH bulletin (In Action) NYS Legislative Update - April 6, 2008 In the last few weeks there has been a lot of activity on the legislative front in NYS. As a recap, a law (Article 165) requiring licensing of all clinical laboratory personnel was passed by the NYS legislation and went into effect in September of 2006 (please visit http://www.op.nysed.gov/clp-cltlic.htm for specific details). Unfortunately, histologists did not have representation on the board that crafted the license nor did we have lobbyists working for our interests. As a result there is no language describing the scope of practice for histologists, no distinction between technician and technologist and no provision for academic training requirements (curriculum). ----- Original Message ----- From: "patsy ruegg" To: "'MaryAnn Dixon'" ; Sent: Sunday, May 25, 2008 10:05 AM Subject: RE: [Histonet] NSH bulletin (In Action) > Please elaborate! > > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. #215 > Aurora, CO 80045 > 720-859-4060 > fax 720-859-4110 > pruegg@ihctech.net > www.ihctech.net > www.ihcrg.org > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > MaryAnn Dixon > Sent: Tuesday, May 20, 2008 1:13 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] NSH bulletin (In Action) > > Hi Histonetters, > > Has anyone read the In Action NSH bulletin lately? Just another > example of the lack of recognition for the field of Histology. I am > new to the field of Histology. What has happened to those employed in > the NY labs? Are other states following by example? Should I get out > now before I am unemployed? > > MaryAnn Dixon > Biological Scientist > Anatomic Pathology > University of Florida Medical Center > 352-392-2235 ext 4517 > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG. > Version: 7.5.524 / Virus Database: 269.24.1/1464 - Release Date: > 5/24/2008 > 8:56 AM > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Tue May 27 10:03:06 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Tue May 27 10:03:06 2008 Subject: [Histonet] Biotin free detection of goat primary antibodies References: <920cc4a70805261955y50c3d198n6d46aa42ba9c20b3@mail.gmail.com> Message-ID: <000c01c8c00a$c4ed2160$6401a8c0@DHXTS541> The avidin/biotin blocking step does not take that much time, 15 minutes for each step and is done BEFORE you apply the primary antibody for overnight staining. It will also depend if your tissue (you did not say what you are staining, kidney, liver, brain???) is one that contains a higher level of endogenous biotin. Omitting the primary antibody is not a good negative control, you should be running a Goat IgG control, at the same concentration as your primary antibody. It could be that biotin is not the cause of your background problem but nonspecific binding of the Goat IgG of your primary antibody to other tissue components. Biocare has a single link polymer kit that is specific for goat but you still need to run a correct negative control, goat IgG. This can be purchased from Jackson Immunoresearch and is not expensive. Jackson now has bovine secondaries, which are supposed to reduce background when working with large animal primary antibodies. Look into this also. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT ----- Original Message ----- From: "Min-Han Tan" To: Sent: Monday, May 26, 2008 8:55 PM Subject: [Histonet] Biotin free detection of goat primary antibodies > Dear all, > > I would like to enquire - I am using a goat primary antibody currently, > and > my negative control (omitting primary antibody) is showing background > staining - this is likely a result of biotin. > > I'd like to enquire if any one is familiar with biotin free systems that > can > detect goat antibodies. A search of the Dako / Envision product line does > not yield any results. I am very reluctant to add an avidin-biotin > blocking > step to what is already an overnight incubation. > > Thanks! > > -- > /min-han > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Tue May 27 11:04:48 2008 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue May 27 11:04:53 2008 Subject: [Histonet] quotes for equipment Vendors please respond Message-ID: I need to get quotes for a forced air slide dryer and for a cassette labeler, such as the MICROWRITER 1. Unfortunately I need them ASAP. Thank you, Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu From Rcartun <@t> harthosp.org Tue May 27 12:26:41 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue May 27 12:27:09 2008 Subject: [Histonet] IF question In-Reply-To: References: Message-ID: <483C0C110200007700002CF7@gwmail6.harthosp.org> We use tonsil, freshly cut. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Anne van Binsbergen" 05/27/08 12:11 AM >>> Hi Histonetters is anyone out there running IF controls - specifically for renals, skins if so what and how? please advise asap thanks Anne -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.m.vanderloos <@t> amc.uva.nl Tue May 27 12:56:28 2008 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Tue May 27 12:56:39 2008 Subject: [Histonet] RE: Biotin free detection of goat primary antibodies Message-ID: Dear Min-Han,We solved this problem with a 3-step approach: goat primary - rabbit anti-goat IgG - anti-rabbit polymer/HRP. The second step we purchased from Southern Biotech Associates, Birmingham, AL. Dilution of the rabbit anti-goat IgG (human IgG adsorbed) came out at 1:2000-5000 (30 min, RT). Any anti-rabbit polymer/HRP will do. Gayle is fully right with respect to the negative control: find a non-immune goat IgG with a known IgG protein concentration. You need that to calculate the final dilution.Hope this helps a bit.Cheers, ChrisChris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The NetherlandsDate: Tue, 27 May 2008 10:55:59 +0800 From: "Min-Han Tan" Subject: [Histonet] Biotin free detection of goat primary antibodies To: histonet@lists.utsouthwestern.edu Dear all, I would like to enquire - I am using a goat primary antibody currently, and my negative control (omitting primary antibody) is showing background staining - this is likely a result of biotin. I'd like to enquire if any one is familiar with biotin free systems that can detect goat antibodies. A search of the Dako / Envision product line does not yield any results. I am very reluctant to add an avidin-biotin blocking step to what is already an overnight incubation. Thanks! -- /min-han From bob.nienhuis <@t> gmail.com Tue May 27 13:04:52 2008 From: bob.nienhuis <@t> gmail.com (Bob Nienhuis) Date: Tue May 27 13:05:01 2008 Subject: [Histonet] Thick section penetration for IHC Message-ID: <45109da50805271104j20ca621ar7abcbf0ddf4f38fb@mail.gmail.com> It has been suggested that the ABC detection system may not be the optimal choice for IHC in thick brain sections (~100 microns). Apparently, the large avidin-biotin complexes may not penetrate tissue too well. What would you suggest instead, LSAB, polymer or...? Bob Nienhuis Neurobiology Research UCLA / VA Medical Center Los Angeles From Shirley_PHUA <@t> hsa.gov.sg Tue May 27 13:03:44 2008 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Tue May 27 13:05:14 2008 Subject: [Histonet] Shirley Phua is out-of-office ... Message-ID: I will be out of the office from 27-05-2008 to 28-05-2008. I'll be away on 27 May 2008 afternoon. Pathologists: I will process your requests when I return. If urgent, please forward your email to Henry_Kyaw@hsa.gov.sg From sprice2003 <@t> gmail.com Tue May 27 15:34:55 2008 From: sprice2003 <@t> gmail.com (Sally Price) Date: Tue May 27 15:35:01 2008 Subject: [Histonet] Biotin free detection of goat primary antibodies Message-ID: Min-Han: I think you're best bet is to try Biocare Medical's anti-Goat polymer detection system -- which can be seen at: http://www.biocaremed.com/promark.html. It is very easy to use and yeilds very clean stains. Cheers, Sally ------------------------------ Message: 4 Date: Tue, 27 May 2008 10:55:59 +0800 From: "Min-Han Tan" Subject: [Histonet] Biotin free detection of goat primary antibodies To: histonet@lists.utsouthwestern.edu Dear all, I would like to enquire - I am using a goat primary antibody currently, and my negative control (omitting primary antibody) is showing background staining - this is likely a result of biotin. I'd like to enquire if any one is familiar with biotin free systems that can detect goat antibodies. A search of the Dako / Envision product line does not yield any results. I am very reluctant to add an avidin-biotin blocking step to what is already an overnight incubation. Thanks! From Warren_Eddings <@t> ssmhc.com Tue May 27 15:59:04 2008 From: Warren_Eddings <@t> ssmhc.com (Warren_Eddings@ssmhc.com) Date: Tue May 27 15:59:29 2008 Subject: [Histonet] evaluation of new antibody cap question 22750 Message-ID: if useing prediluted antibodys thanks warren_eddings@ssmhc.co _________________________________________________________________ Confiden attachments, is for the s may contain confidential and privi unauthorized review, use, disclosure or distributi prohibited. If you are not the intended recipient, please contact t he sender by reply email and destroy all copies of the original message. From dcojita <@t> tampabay.rr.com Tue May 27 16:11:31 2008 From: dcojita <@t> tampabay.rr.com (dcojita@tampabay.rr.com) Date: Tue May 27 16:11:49 2008 Subject: [Histonet] KOH for nails In-Reply-To: Message-ID: Does anyone have a procedure they would be willing to share for KOH (looking for nail fungus)? Is this considered an AP or a clinical test? What is the CPT code? Any information you have would be greatly appreciated! From Karen_Skish <@t> rush.edu Tue May 27 15:45:24 2008 From: Karen_Skish <@t> rush.edu (Karen_Skish@rush.edu) Date: Tue May 27 16:16:49 2008 Subject: [Histonet] Rate of formalin penetration in human brain sections Message-ID: Hi-- One of our investigators is interested in the approximate rate of fixation of human brain tissue, independent of any formaldehyde diffusion effects. In other words, in a very small or very thin piece of human brain tissue, what is the fixation rate? He found published data for rat kidney, but would like to try to at least determine if the fixation rate should be higher or lower in human brain tissue. He is looking for data for room temperature, but any information would be greatly appreciated. Thanks! Karen M Skish, MS, PA(ASCP)MT Pathologists' Assistant & Manager, Neuropathology Lab Rush Alzheimer's Disease Center Cohn Research Building, Lab 441 1735 West Harrison Street Chicago IL 60612 From mickie25 <@t> netzero.net Tue May 27 20:22:56 2008 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Tue May 27 20:22:44 2008 Subject: [Histonet] IF question In-Reply-To: <483C0C110200007700002CF7@gwmail6.harthosp.org> References: <483C0C110200007700002CF7@gwmail6.harthosp.org> Message-ID: Hello Histonetters, I saw that Jan Mahoney gave a talk on Lean Histology a couple of weeks ago at the Illinois Histology Society seminar and was wondering if she would give me a shout as I have someone who is interested in what the buzz is about. Me too for that matter. Thanks Jan! Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From AnthonyH <@t> chw.edu.au Tue May 27 23:10:12 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue May 27 23:10:26 2008 Subject: [Histonet] Reaction between Haemoglobin and acetic acid Message-ID: Hi all, I have been asked what is the altered haemoglobin called that results from the treatment of tissues with acetic acid. All I could think of was that carbon monoxide reacts with haemoglobin to form carboxyhemoglobin. Any ideas? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From kemlo <@t> f2s.com Wed May 28 03:11:42 2008 From: kemlo <@t> f2s.com (kemlo) Date: Wed May 28 03:12:26 2008 Subject: [Histonet] Reaction between Haemoglobin and acetic acid In-Reply-To: References: Message-ID: <72927951B67343EE84B30090C88C3D2B@KemloPC> SUMMARY It is shown by sedimentation velocity measurements that the presence of undissociated acetic acid favors the dissociation of hemoglobin in a pH region in which hydrogen ion had previously been thought to have the dominant effect. Acknowledgments-We wish to acknowledge continuous discussion and collaboration with Professors J. Wyman and E. Antonini during the course of this work. REFEREYCES 1. REITHEL, F. J., Advances in Protein Chem., 18, 123 (1963). 2. PEDERSEN, K. O., AND ANDERSSON, K. J. I., in T. SVEDBERG AND K. 0. PEDERSEN (Editors), The ultracentrifuge, Oxford University Press, London, 1940, p. 407. 3. ROSSI-FANELLI, A., ANTONINI, E., AND CAPUTO, A., Advances in Protein Chem., 19, 73 (1964). 4. PERUTZ, M. F., Biochem. J., 94, 21P (1965). 5. CULLIS, A. F., MUIRHEAD, H., PERUTZ, M. F., ROSSMANN, M. G., AND NORTH, A. C. T., Proc. Roy. Sot. London, Ser. A, 265, 161 (1962). 6. PHELPS, R. A., AND CANN, J. R., J. Am. Chem. Sot., 78, 3539 (1956). 7. CANN, J. R., J. Biol. Chem., 235, 2810 (1960). 8. CANN, J. R., AND GOAD, W. B., J. Biol. Chem., 240, 148 (1965). 9. FIELD, E. O., AND O'BRIEN, J. R., Bioehem. J., 60, 656 (1955). 10. ROSSI-FANELLI, A., ANTONINI, E., AND CAPUTO, A., J. Biol. Chem., 236, 391 (1961). 11. LONG, C. (Editor), Biochemists' handbook, Spon Ltd., London, 1961. 12. PEDERSEN, K. O., J. Phys. Chem., 62, 1282 (1958). Downloaded from www.jbc.org by on May 28, 2008 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: 28 May 2008 05:10 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Reaction between Haemoglobin and acetic acid Hi all, I have been asked what is the altered haemoglobin called that results from the treatment of tissues with acetic acid. All I could think of was that carbon monoxide reacts with haemoglobin to form carboxyhemoglobin. Any ideas? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NHeath <@t> Lifespan.org Wed May 28 05:52:00 2008 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Wed May 28 05:52:06 2008 Subject: [Histonet] nerve bx Message-ID: <130E8991F210424096EFC6F42EA33B2402B63361@LSCOEXCH1.lsmaster.lifespan.org> Hi, Does anyone know of a procedure for nerve biopsies/nerve teasing that does not use Osmium Tetroxide?? Tx, Nancy From rjbuesa <@t> yahoo.com Wed May 28 07:28:37 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 28 07:28:41 2008 Subject: [Histonet] Rate of formalin penetration in human brain sections In-Reply-To: Message-ID: <216952.98289.qm@web65705.mail.ac4.yahoo.com> I think that this is a very interesting subject for your investigator to research by himself/herself (especially independent of any formaldehyde diffusion effects)! Ren? J. Karen_Skish@rush.edu wrote: Hi-- One of our investigators is interested in the approximate rate of fixation of human brain tissue, independent of any formaldehyde diffusion effects. In other words, in a very small or very thin piece of human brain tissue, what is the fixation rate? He found published data for rat kidney, but would like to try to at least determine if the fixation rate should be higher or lower in human brain tissue. He is looking for data for room temperature, but any information would be greatly appreciated. Thanks! Karen M Skish, MS, PA(ASCP)MT Pathologists' Assistant & Manager, Neuropathology Lab Rush Alzheimer's Disease Center Cohn Research Building, Lab 441 1735 West Harrison Street Chicago IL 60612 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed May 28 07:33:23 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 28 07:33:30 2008 Subject: [Histonet] KOH for nails In-Reply-To: Message-ID: <895728.9462.qm@web65706.mail.ac4.yahoo.com> KOH is used (at 10% for 30 minutes) to soften nails so they can be properly infiltrated and cut. That is an AP procedure (although it is NOT a decalcification code I always used CPT code 88331). Nail fungus will require a fungus stain (I always used PAS) and this is an AP test for organisms identification (CPT code 88312) Ren? J. dcojita@tampabay.rr.com wrote: Does anyone have a procedure they would be willing to share for KOH (looking for nail fungus)? Is this considered an AP or a clinical test? What is the CPT code? Any information you have would be greatly appreciated! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed May 28 07:40:44 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 28 07:40:51 2008 Subject: [Histonet] Reaction between Haemoglobin and acetic acid In-Reply-To: Message-ID: <113899.56959.qm@web65716.mail.ac4.yahoo.com> In dilute solution (1%) acetic acid destroys red blood cells to facilitate the examination of white blood cells. At this conc. it separates the dermis from the epidermis. Ren? J. Tony Henwood wrote: Hi all, I have been asked what is the altered haemoglobin called that results from the treatment of tissues with acetic acid. All I could think of was that carbon monoxide reacts with haemoglobin to form carboxyhemoglobin. Any ideas? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nmeres <@t> gmu.edu Wed May 28 08:52:55 2008 From: nmeres <@t> gmu.edu (Norman Meres) Date: Wed May 28 08:53:14 2008 Subject: [Histonet] Cryostat in the Washington, DC area? Message-ID: Hello: The cryostat that I was planning on using for my research is not available at the moment. Is there anyone in the Washington, DC area that would have one that I could use? I am sectioning epidermis of lobsters. Thanks, Norman Meres Doctoral Candidate Environmental Science and Public Policy George Mason University From nancy_schmitt <@t> pa-ucl.com Wed May 28 09:08:47 2008 From: nancy_schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Wed May 28 09:09:03 2008 Subject: [Histonet] heat + formalin Message-ID: <9FC023A4AB52BB4D87DC6456081A822C087CA1@mercury.pa-ucl.com> Good Morning Need some input, please. Does anyone combine heat during the formalin part of processing? We are having some issues with breast cases that need to be read out the next am - we are running them on a shorter processing schedule (done at 4 am) - and not having the best results.We use a closed system Tissue-Tek VIP 3000. Is anybody using microwave fixation? I think we will just put them on the longer processor (done at 0630) and be done - but wanted to check for other options. Thanks in advance Nancy Schmitt Histology Coordinator Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From mcauliff <@t> umdnj.edu Wed May 28 09:19:20 2008 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed May 28 09:19:05 2008 Subject: [Histonet] Rate of formalin penetration in human brain sections In-Reply-To: References: Message-ID: <483D69E8.8020506@umdnj.edu> Hi Karen: Formalin fixes tissue slowly, even very thin slices. This has been known for many, many years. The work of Medwar and of John R. Baker (Principles of Biological Technique, John Wiley and Sons, 1958) comes to mind. Perhaps there are slight differences between kidney and brain but my guess is that if there is a difference it is insignificant. Before trying to design (how are you going to define fixation?) and perform such investigations I suggest a trip to the library. I doubt if the information you seek is on line, it is too old. However, do not confuse "old" with "out dated" or "bad". Good luck. Geoff Karen_Skish@rush.edu wrote: > Hi-- > One of our investigators is interested in the approximate rate of fixation > of human brain tissue, independent of any formaldehyde diffusion effects. > In other words, in a very small or very thin piece of human brain tissue, > what is the fixation rate? He found published data for rat kidney, but > would like to try to at least determine if the fixation rate should be > higher or lower in human brain tissue. He is looking for data for room > temperature, but any information would be greatly appreciated. > Thanks! > Karen M Skish, MS, PA(ASCP)MT > Pathologists' Assistant & Manager, Neuropathology Lab > Rush Alzheimer's Disease Center > Cohn Research Building, Lab 441 > 1735 West Harrison Street > Chicago IL 60612 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From bhewlett <@t> cogeco.ca Wed May 28 09:29:38 2008 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Wed May 28 09:29:50 2008 Subject: [Histonet] Rate of formalin penetration in human brain sections References: Message-ID: <001301c8c0cf$430e19e0$6700a8c0@mainbox> Karen, The formaldehyde fixation rate of tissue is determined from a combination of the penetration rate, which is governed by diffusibilty, the maximal covalent formaldehyde binding time, which is governed by the formaldehyde 'clock' reaction, and the slow subsequent cross-linking which then occurs, This slow cross-linking is thought to be almost complete by 7 days post formaldehyde binding time, but can continue over longer time periods. What your investigator has found, is probably the published data related to the maximal covalent formaldehyde binding time, i.e. 24 hours at room temperature as determined by Fox et al (reference #1). This study was essentially independent of diffusibilty, i.e. negligible penetration time, since 16 micrometer thick sections of rat kidney were the substrate. However, this study does not take into account subsequent further cross-linking. A study by Helander (reference #2) determined the maximal covalent formaldehyde binding time as 25 hours. This was performed on 4 mm thick slices of rabbit liver, so diffusibility has to be taken into account. In addition, this study also partially looked at subsequent further cross-linking in relationship to reversibility. A later study by Helander (reference #3) compared the maximal covalent formaldehyde binding time between kidney and brain tissues. This was stated to be 50 hours however, the tissues thickness was increased to 8 mm and so the diffusibilty effect has to be taken into account even more. Reference #1. Fox CH., et.al. Formaldehyde fixation. J Histochem. Cytochem. 1985; 33, 845 -853 Reference #2 Helander, KG. Kinetic studies of formaldehyde binding in tissue. Biotechnique and Histochemistry. 1994; 69, 177 -179 Reference #3 Helander, K.G. Formaldehyde binding in Brain and Kidney: A kinetic study of fixation. The Journal of Histotechnology. 1999; 22(4), 317-318. Regards, Bryan ----- Original Message ----- From: To: Sent: Tuesday, May 27, 2008 4:45 PM Subject: [Histonet] Rate of formalin penetration in human brain sections > Hi-- > One of our investigators is interested in the approximate rate of fixation > of human brain tissue, independent of any formaldehyde diffusion effects. > In other words, in a very small or very thin piece of human brain tissue, > what is the fixation rate? He found published data for rat kidney, but > would like to try to at least determine if the fixation rate should be > higher or lower in human brain tissue. He is looking for data for room > temperature, but any information would be greatly appreciated. > Thanks! > Karen M Skish, MS, PA(ASCP)MT > Pathologists' Assistant & Manager, Neuropathology Lab > Rush Alzheimer's Disease Center > Cohn Research Building, Lab 441 > 1735 West Harrison Street > Chicago IL 60612 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From CIngles <@t> uwhealth.org Wed May 28 09:30:08 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Wed May 28 09:31:42 2008 Subject: [Histonet] NSH teleconference today Message-ID: <08A0A863637F1349BBFD83A96B27A50A120138@uwhis-xchng3.uwhis.hosp.wisc.edu> Help! I don't seem to have recieved the e-mail giving me the site to download the lecture materials. Anyone have it? Claire From jgutierrez <@t> precisionpath.us Wed May 28 09:33:31 2008 From: jgutierrez <@t> precisionpath.us (Juan Gutierrez) Date: Wed May 28 09:33:41 2008 Subject: [Histonet] heat + formalin In-Reply-To: <9FC023A4AB52BB4D87DC6456081A822C087CA1@mercury.pa-ucl.com> References: <9FC023A4AB52BB4D87DC6456081A822C087CA1@mercury.pa-ucl.com> Message-ID: <004201c8c0cf$ce555040$6a00a8c0@precisionpath.lcl> Hi Nancy, if you are doing breast prognostic markers on your cases, you might want to let it fix overnight before processing. The FDA requires it for Her-2 testing. No we do not use heat on our formalin steps, but we require our docs to cut tissue at 3mm or less thickness. So far this has been working fine with fixing and infiltration. Good luck, Juan C. Gutierrez, HT(ASCP) Precision Pathology Services 210.646.0890 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Wednesday, May 28, 2008 9:09 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] heat + formalin Good Morning Need some input, please. Does anyone combine heat during the formalin part of processing? We are having some issues with breast cases that need to be read out the next am - we are running them on a shorter processing schedule (done at 4 am) - and not having the best results.We use a closed system Tissue-Tek VIP 3000. Is anybody using microwave fixation? I think we will just put them on the longer processor (done at 0630) and be done - but wanted to check for other options. Thanks in advance Nancy Schmitt Histology Coordinator Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed May 28 09:45:20 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 28 09:45:27 2008 Subject: [Histonet] heat + formalin In-Reply-To: <9FC023A4AB52BB4D87DC6456081A822C087CA1@mercury.pa-ucl.com> Message-ID: <743811.30001.qm@web65704.mail.ac4.yahoo.com> I never used heat with the formalin because fumes issues (if by any chance we had to open the retort). You should not run breast in short cycles unless the slices are extremely thin and you increase the time in the clearing agent (taking away from the alcohols). It is better to have a good section a few hours later, than a useless section rapidly. Ren? J. Nancy Schmitt wrote: Good Morning Need some input, please. Does anyone combine heat during the formalin part of processing? We are having some issues with breast cases that need to be read out the next am - we are running them on a shorter processing schedule (done at 4 am) - and not having the best results.We use a closed system Tissue-Tek VIP 3000. Is anybody using microwave fixation? I think we will just put them on the longer processor (done at 0630) and be done - but wanted to check for other options. Thanks in advance Nancy Schmitt Histology Coordinator Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From stephanie.d.rivera <@t> gsk.com Wed May 28 10:12:43 2008 From: stephanie.d.rivera <@t> gsk.com (stephanie.d.rivera@gsk.com) Date: Wed May 28 10:13:13 2008 Subject: [Histonet] Formalin alternative fixative for rodent and large animal Message-ID: Hello histoworld, Does anyone have any suggestions for altenative fixative to formalin? I still need to maintain quality of IHC and H&E stain and morphology. I've come across Prefer and Notoxhisto. Are there any others for animal tissue that anyone have tried? Stephanie D. Rivera Safety Assessment Department GlaxoSmithKline 709 Swedeland RD King of Prussia, PA 19406 phone: 610-270-7340 fax: 610-270-7202 From nicole.walsh <@t> umassmed.edu Wed May 28 10:20:18 2008 From: nicole.walsh <@t> umassmed.edu (Nicole C Walsh) Date: Wed May 28 10:20:57 2008 Subject: [Histonet] Experiencing Chatter in paraffin ribbons Message-ID: <94A5E686-2355-422F-8F12-27F149E0EAB8@umassmed.edu> Hi, We routinely cut 5micron paraffin sections of decalcified mouse bones including whole hindpaws. We use Surgipath Infiltration medium for infiltration steps and then Surgipath EM-400 Embedding paraffin for embedding. This has worked well in the past, but since moving to a new institution and using different microtome setup we are now having problems with sectioning. Particularly we are experiencing a lot of chatter in the surrounding wax. We routinely keep the blocks cold when sectioning.. cooling in between each ribbon of 5-7 sections (maintained at 4degC on cooling block on embedding station, with slight coating of block softener (60% glycerol, 20% ethanol and 20% water)). The tissue generally comes off intact suggesting that infiltration of the tissue is reasonable. We are trying to eliminate possible reasons for our problems. A couple of things have changed since moving here.. We recently refreshed our paraffin supplies and this seems to have been when our troubles really started. But Surgipath have said that they have not changed the formula, but have given us paraffin from a different lot number to try. We've had a maintenance rep recently service the microtome (Microm HM315) and he could not find reason for the chatter (we section with angle set to 10 as this is what the rep suggested should be used for this microtome, and have tried varying the angle previously with no success) We used to use surgipath high profile blades (non-coated) with success (and I've seen discussion on histonet to say that the high profile blades are better for hard tissue). But the microtome that we now use will only take low-profile blades and so far we've tried the Richard Allan Scientific low profile blades (found that these became blunt quickly) and are now trying the teflon coated low profile blades from surgipath. If anyone has any suggestions for things that we might try that could help correct our problems we would be very grateful. Thanks in advance, Nicole From JMahoney <@t> alegent.org Wed May 28 10:28:51 2008 From: JMahoney <@t> alegent.org (Mahoney,Janice A) Date: Wed May 28 10:31:10 2008 Subject: [Histonet] RE: NSH teleconference today In-Reply-To: <08A0A863637F1349BBFD83A96B27A50A120138@uwhis-xchng3.uwhis.hosp.wisc.edu> References: <08A0A863637F1349BBFD83A96B27A50A120138@uwhis-xchng3.uwhis.hosp.wisc.edu> Message-ID: <346E5878979BA54FB4B0BFD6AD93B9B9B01EE96359@EXCHMBC1.ad.ah.local> Me either!!!!! Janice Mahoney HT(ASCP) Histology/Cytology Coordinator Alegent Health Laboratory 4955 F Street Omaha, NE (402)717-2889 fax(402)717-5231 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire [CIngles@uwhealth.org] Sent: Wednesday, May 28, 2008 9:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NSH teleconference today Help! I don't seem to have recieved the e-mail giving me the site to download the lecture materials. Anyone have it? Claire _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From Terry.Marshall <@t> rothgen.nhs.uk Wed May 28 10:31:11 2008 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Wed May 28 10:31:48 2008 Subject: [Histonet] Rate of formalin penetration in human brain sections Message-ID: <5C0BED61F529364E86309CADEA63FEF20163F40A@TRFT-EX01.xRothGen.nhs.uk> Agree with Geoff entirely. In particular you will not find it possible to define an end-point for formalin fixation. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff McAuliffe Sent: 28 May 2008 15:19 To: Karen_Skish@rush.edu Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Rate of formalin penetration in human brain sections Hi Karen: Formalin fixes tissue slowly, even very thin slices. This has been known for many, many years. The work of Medwar and of John R. Baker (Principles of Biological Technique, John Wiley and Sons, 1958) comes to mind. Perhaps there are slight differences between kidney and brain but my guess is that if there is a difference it is insignificant. Before trying to design (how are you going to define fixation?) and perform such investigations I suggest a trip to the library. I doubt if the information you seek is on line, it is too old. However, do not confuse "old" with "out dated" or "bad". Good luck. Geoff Karen_Skish@rush.edu wrote: > Hi-- > One of our investigators is interested in the approximate rate of > fixation of human brain tissue, independent of any formaldehyde diffusion effects. > In other words, in a very small or very thin piece of human brain > tissue, what is the fixation rate? He found published data for rat > kidney, but would like to try to at least determine if the fixation > rate should be higher or lower in human brain tissue. He is looking > for data for room temperature, but any information would be greatly appreciated. > Thanks! > Karen M Skish, MS, PA(ASCP)MT > Pathologists' Assistant & Manager, Neuropathology Lab Rush Alzheimer's > Disease Center Cohn Research Building, Lab 441 > 1735 West Harrison Street > Chicago IL 60612 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Wed May 28 10:32:33 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed May 28 10:32:48 2008 Subject: [Histonet] Formalin alternative fixative for rodent and large animal In-Reply-To: Message-ID: In my opinion, for pharma research, there is no worthwhile formalin substitute that will give consistent IHC results. I have tried STF (which was discontinued) and other subs - finally went back to formalin. With STF, the morphology of routine H+E's was horrible. I'm sure there are differing opinions out there - after all, this is histonet. Jackie O' stephanie.d.rivera@gsk.com Sent by: histonet-bounces@lists.utsouthwestern.edu 05/28/2008 10:12 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Formalin alternative fixative for rodent and large animal Hello histoworld, Does anyone have any suggestions for altenative fixative to formalin? I still need to maintain quality of IHC and H&E stain and morphology. I've come across Prefer and Notoxhisto. Are there any others for animal tissue that anyone have tried? Stephanie D. Rivera Safety Assessment Department GlaxoSmithKline 709 Swedeland RD King of Prussia, PA 19406 phone: 610-270-7340 fax: 610-270-7202 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Carrie <@t> nsh.org Wed May 28 10:43:07 2008 From: Carrie <@t> nsh.org (Carrie Diamond) Date: Wed May 28 10:43:21 2008 Subject: [Histonet] RE: NSH Teleconference Today In-Reply-To: References: Message-ID: Hello - If you have questions regarding the NSH Teleconference, please contact the NSH office at 443.535.4060 or via email at histo@nsh.org. Thank you, Carrie Diamond Executive Director National Society for Histotechnology 10320 Little Patuxent Parkway Suite 804 Columbia, MD 21044 P: 443.535.4060 Direct: 443.535.4066 Fax: 443.535.4055 E-mail: carrie@nsh.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, May 28, 2008 11:34 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 54, Issue 41 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: IF question (Richard Cartun) 2. RE: Biotin free detection of goat primary antibodies (C.M. van der Loos) 3. Thick section penetration for IHC (Bob Nienhuis) 4. Shirley Phua is out-of-office ... (Shirley PHUA) 5. RE: Biotin free detection of goat primary antibodies (Sally Price) 6. evaluation of new antibody cap question 22750 (Warren_Eddings@ssmhc.com) 7. KOH for nails (dcojita@tampabay.rr.com) 8. Rate of formalin penetration in human brain sections (Karen_Skish@rush.edu) 9. RE: IF question (Mickie Johnson) 10. Reaction between Haemoglobin and acetic acid (Tony Henwood) 11. RE: Reaction between Haemoglobin and acetic acid (kemlo) 12. nerve bx (Heath, Nancy L.) 13. Re: Rate of formalin penetration in human brain sections (Rene J Buesa) 14. Re: KOH for nails (Rene J Buesa) 15. Re: Reaction between Haemoglobin and acetic acid (Rene J Buesa) 16. Cryostat in the Washington, DC area? (Norman Meres) 17. heat + formalin (Nancy Schmitt) 18. Re: Rate of formalin penetration in human brain sections (Geoff McAuliffe) 19. Re: Rate of formalin penetration in human brain sections (Bryan Hewlett) 20. NSH teleconference today (Ingles Claire) 21. RE: heat + formalin (Juan Gutierrez) 22. Re: heat + formalin (Rene J Buesa) 23. Formalin alternative fixative for rodent and large animal (stephanie.d.rivera@gsk.com) 24. Experiencing Chatter in paraffin ribbons (Nicole C Walsh) 25. RE: NSH teleconference today (Mahoney,Janice A) 26. RE: Rate of formalin penetration in human brain sections (Marshall Terry Dr, Consultant Histopathologist) ---------------------------------------------------------------------- Message: 1 Date: Tue, 27 May 2008 13:26:41 -0400 From: "Richard Cartun" Subject: Re: [Histonet] IF question To: "Anne van Binsbergen" , "histonet@lists.utsouthwestern.edu" , Message-ID: <483C0C110200007700002CF7@gwmail6.harthosp.org> Content-Type: text/plain; charset=US-ASCII We use tonsil, freshly cut. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Anne van Binsbergen" 05/27/08 12:11 AM >>> Hi Histonetters is anyone out there running IF controls - specifically for renals, skins if so what and how? please advise asap thanks Anne -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Tue, 27 May 2008 19:56:28 +0200 From: "C.M. van der Loos" Subject: [Histonet] RE: Biotin free detection of goat primary antibodies To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Dear Min-Han,We solved this problem with a 3-step approach: goat primary - rabbit anti-goat IgG - anti-rabbit polymer/HRP. The second step we purchased from Southern Biotech Associates, Birmingham, AL. Dilution of the rabbit anti-goat IgG (human IgG adsorbed) came out at 1:2000-5000 (30 min, RT). Any anti-rabbit polymer/HRP will do. Gayle is fully right with respect to the negative control: find a non-immune goat IgG with a known IgG protein concentration. You need that to calculate the final dilution.Hope this helps a bit.Cheers, ChrisChris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The NetherlandsDate: Tue, 27 May 2008 10:55:59 +0800 From: "Min-Han Tan" Subject: [Histonet] Biotin free detection of goat primary antibodies To: histonet@lists.utsouthwestern.edu Dear all, I would like to enquire - I am using a goat primary antibody currently, and my negative control (omitting primary antibody) is showing background staining - this is likely a result of biotin. I'd like to enquire if any one is familiar with biotin free systems that can detect goat antibodies. A search of the Dako / Envision product line does not yield any results. I am very reluctant to add an avidin-biotin blocking step to what is already an overnight incubation. Thanks! -- /min-han ------------------------------ Message: 3 Date: Tue, 27 May 2008 10:04:52 -0800 From: "Bob Nienhuis" Subject: [Histonet] Thick section penetration for IHC To: Histonet Message-ID: <45109da50805271104j20ca621ar7abcbf0ddf4f38fb@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 It has been suggested that the ABC detection system may not be the optimal choice for IHC in thick brain sections (~100 microns). Apparently, the large avidin-biotin complexes may not penetrate tissue too well. What would you suggest instead, LSAB, polymer or...? Bob Nienhuis Neurobiology Research UCLA / VA Medical Center Los Angeles ------------------------------ Message: 4 Date: Wed, 28 May 2008 02:03:44 +0800 From: Shirley PHUA Subject: [Histonet] Shirley Phua is out-of-office ... To: histonet Message-ID: Content-Type: text/plain; charset=US-ASCII I will be out of the office from 27-05-2008 to 28-05-2008. I'll be away on 27 May 2008 afternoon. Pathologists: I will process your requests when I return. If urgent, please forward your email to Henry_Kyaw@hsa.gov.sg ------------------------------ Message: 5 Date: Tue, 27 May 2008 16:34:55 -0400 From: "Sally Price" Subject: RE: [Histonet] Biotin free detection of goat primary antibodies To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Min-Han: I think you're best bet is to try Biocare Medical's anti-Goat polymer detection system -- which can be seen at: http://www.biocaremed.com/promark.html. It is very easy to use and yeilds very clean stains. Cheers, Sally ------------------------------ Message: 4 Date: Tue, 27 May 2008 10:55:59 +0800 From: "Min-Han Tan" Subject: [Histonet] Biotin free detection of goat primary antibodies To: histonet@lists.utsouthwestern.edu Dear all, I would like to enquire - I am using a goat primary antibody currently, and my negative control (omitting primary antibody) is showing background staining - this is likely a result of biotin. I'd like to enquire if any one is familiar with biotin free systems that can detect goat antibodies. A search of the Dako / Envision product line does not yield any results. I am very reluctant to add an avidin-biotin blocking step to what is already an overnight incubation. Thanks! ------------------------------ Message: 6 Date: Tue, 27 May 2008 15:59:04 -0500 From: Warren_Eddings@ssmhc.com Subject: [Histonet] evaluation of new antibody cap question 22750 To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="ISO-8859-1" if useing prediluted antibodys thanks warren_eddings@ssmhc.co _________________________________________________________________ Confiden attachments, is for the s may contain confidential and privi unauthorized review, use, disclosure or distributi prohibited. If you are not the intended recipient, please contact t he sender by reply email and destroy all copies of the original message. ------------------------------ Message: 7 Date: Tue, 27 May 2008 17:11:31 -0400 From: Subject: [Histonet] KOH for nails To: "'histonet@lists.utsouthwestern.edu'" , Message-ID: Content-Type: text/plain; charset="us-ascii" Does anyone have a procedure they would be willing to share for KOH (looking for nail fungus)? Is this considered an AP or a clinical test? What is the CPT code? Any information you have would be greatly appreciated! ------------------------------ Message: 8 Date: Tue, 27 May 2008 15:45:24 -0500 From: Karen_Skish@rush.edu Subject: [Histonet] Rate of formalin penetration in human brain sections To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi-- One of our investigators is interested in the approximate rate of fixation of human brain tissue, independent of any formaldehyde diffusion effects. In other words, in a very small or very thin piece of human brain tissue, what is the fixation rate? He found published data for rat kidney, but would like to try to at least determine if the fixation rate should be higher or lower in human brain tissue. He is looking for data for room temperature, but any information would be greatly appreciated. Thanks! Karen M Skish, MS, PA(ASCP)MT Pathologists' Assistant & Manager, Neuropathology Lab Rush Alzheimer's Disease Center Cohn Research Building, Lab 441 1735 West Harrison Street Chicago IL 60612 ------------------------------ Message: 9 Date: Tue, 27 May 2008 18:22:56 -0700 From: "Mickie Johnson" Subject: RE: [Histonet] IF question To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Hello Histonetters, I saw that Jan Mahoney gave a talk on Lean Histology a couple of weeks ago at the Illinois Histology Society seminar and was wondering if she would give me a shout as I have someone who is interested in what the buzz is about. Me too for that matter. Thanks Jan! Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. ------------------------------ Message: 10 Date: Wed, 28 May 2008 14:10:12 +1000 From: "Tony Henwood" Subject: [Histonet] Reaction between Haemoglobin and acetic acid To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi all, I have been asked what is the altered haemoglobin called that results from the treatment of tissues with acetic acid. All I could think of was that carbon monoxide reacts with haemoglobin to form carboxyhemoglobin. Any ideas? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 11 Date: Wed, 28 May 2008 09:11:42 +0100 From: "kemlo" Subject: RE: [Histonet] Reaction between Haemoglobin and acetic acid To: "'Tony Henwood'" , Message-ID: <72927951B67343EE84B30090C88C3D2B@KemloPC> Content-Type: text/plain; charset="US-ASCII" SUMMARY It is shown by sedimentation velocity measurements that the presence of undissociated acetic acid favors the dissociation of hemoglobin in a pH region in which hydrogen ion had previously been thought to have the dominant effect. Acknowledgments-We wish to acknowledge continuous discussion and collaboration with Professors J. Wyman and E. Antonini during the course of this work. REFEREYCES 1. REITHEL, F. J., Advances in Protein Chem., 18, 123 (1963). 2. PEDERSEN, K. O., AND ANDERSSON, K. J. I., in T. SVEDBERG AND K. 0. PEDERSEN (Editors), The ultracentrifuge, Oxford University Press, London, 1940, p. 407. 3. ROSSI-FANELLI, A., ANTONINI, E., AND CAPUTO, A., Advances in Protein Chem., 19, 73 (1964). 4. PERUTZ, M. F., Biochem. J., 94, 21P (1965). 5. CULLIS, A. F., MUIRHEAD, H., PERUTZ, M. F., ROSSMANN, M. G., AND NORTH, A. C. T., Proc. Roy. Sot. London, Ser. A, 265, 161 (1962). 6. PHELPS, R. A., AND CANN, J. R., J. Am. Chem. Sot., 78, 3539 (1956). 7. CANN, J. R., J. Biol. Chem., 235, 2810 (1960). 8. CANN, J. R., AND GOAD, W. B., J. Biol. Chem., 240, 148 (1965). 9. FIELD, E. O., AND O'BRIEN, J. R., Bioehem. J., 60, 656 (1955). 10. ROSSI-FANELLI, A., ANTONINI, E., AND CAPUTO, A., J. Biol. Chem., 236, 391 (1961). 11. LONG, C. (Editor), Biochemists' handbook, Spon Ltd., London, 1961. 12. PEDERSEN, K. O., J. Phys. Chem., 62, 1282 (1958). Downloaded from www.jbc.org by on May 28, 2008 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: 28 May 2008 05:10 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Reaction between Haemoglobin and acetic acid Hi all, I have been asked what is the altered haemoglobin called that results from the treatment of tissues with acetic acid. All I could think of was that carbon monoxide reacts with haemoglobin to form carboxyhemoglobin. Any ideas? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Wed, 28 May 2008 06:52:00 -0400 From: "Heath, Nancy L." Subject: [Histonet] nerve bx To: Message-ID: <130E8991F210424096EFC6F42EA33B2402B63361@LSCOEXCH1.lsmaster.lifespan.org> Content-Type: text/plain; charset="us-ascii" Hi, Does anyone know of a procedure for nerve biopsies/nerve teasing that does not use Osmium Tetroxide?? Tx, Nancy ------------------------------ Message: 13 Date: Wed, 28 May 2008 05:28:37 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Rate of formalin penetration in human brain sections To: Karen_Skish@rush.edu, histonet@lists.utsouthwestern.edu Message-ID: <216952.98289.qm@web65705.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I think that this is a very interesting subject for your investigator to research by himself/herself (especially independent of any formaldehyde diffusion effects)! Ren? J. Karen_Skish@rush.edu wrote: Hi-- One of our investigators is interested in the approximate rate of fixation of human brain tissue, independent of any formaldehyde diffusion effects. In other words, in a very small or very thin piece of human brain tissue, what is the fixation rate? He found published data for rat kidney, but would like to try to at least determine if the fixation rate should be higher or lower in human brain tissue. He is looking for data for room temperature, but any information would be greatly appreciated. Thanks! Karen M Skish, MS, PA(ASCP)MT Pathologists' Assistant & Manager, Neuropathology Lab Rush Alzheimer's Disease Center Cohn Research Building, Lab 441 1735 West Harrison Street Chicago IL 60612 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Wed, 28 May 2008 05:33:23 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] KOH for nails To: dcojita@tampabay.rr.com, "'histonet@lists.utsouthwestern.edu'" , histonet-request@lists.utsouthwestern.edu Message-ID: <895728.9462.qm@web65706.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 KOH is used (at 10% for 30 minutes) to soften nails so they can be properly infiltrated and cut. That is an AP procedure (although it is NOT a decalcification code I always used CPT code 88331). Nail fungus will require a fungus stain (I always used PAS) and this is an AP test for organisms identification (CPT code 88312) Ren? J. dcojita@tampabay.rr.com wrote: Does anyone have a procedure they would be willing to share for KOH (looking for nail fungus)? Is this considered an AP or a clinical test? What is the CPT code? Any information you have would be greatly appreciated! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Wed, 28 May 2008 05:40:44 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Reaction between Haemoglobin and acetic acid To: Tony Henwood , Histonet@lists.utsouthwestern.edu Message-ID: <113899.56959.qm@web65716.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 In dilute solution (1%) acetic acid destroys red blood cells to facilitate the examination of white blood cells. At this conc. it separates the dermis from the epidermis. Ren? J. Tony Henwood wrote: Hi all, I have been asked what is the altered haemoglobin called that results from the treatment of tissues with acetic acid. All I could think of was that carbon monoxide reacts with haemoglobin to form carboxyhemoglobin. Any ideas? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Wed, 28 May 2008 09:52:55 -0400 From: Norman Meres Subject: [Histonet] Cryostat in the Washington, DC area? To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes Hello: The cryostat that I was planning on using for my research is not available at the moment. Is there anyone in the Washington, DC area that would have one that I could use? I am sectioning epidermis of lobsters. Thanks, Norman Meres Doctoral Candidate Environmental Science and Public Policy George Mason University ------------------------------ Message: 17 Date: Wed, 28 May 2008 09:08:47 -0500 From: Nancy Schmitt Subject: [Histonet] heat + formalin To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <9FC023A4AB52BB4D87DC6456081A822C087CA1@mercury.pa-ucl.com> Content-Type: text/plain; charset="iso-8859-1" Good Morning Need some input, please. Does anyone combine heat during the formalin part of processing? We are having some issues with breast cases that need to be read out the next am - we are running them on a shorter processing schedule (done at 4 am) - and not having the best results.We use a closed system Tissue-Tek VIP 3000. Is anybody using microwave fixation? I think we will just put them on the longer processor (done at 0630) and be done - but wanted to check for other options. Thanks in advance Nancy Schmitt Histology Coordinator Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ------------------------------ Message: 18 Date: Wed, 28 May 2008 10:19:20 -0400 From: Geoff McAuliffe Subject: Re: [Histonet] Rate of formalin penetration in human brain sections To: Karen_Skish@rush.edu Cc: histonet@lists.utsouthwestern.edu Message-ID: <483D69E8.8020506@umdnj.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hi Karen: Formalin fixes tissue slowly, even very thin slices. This has been known for many, many years. The work of Medwar and of John R. Baker (Principles of Biological Technique, John Wiley and Sons, 1958) comes to mind. Perhaps there are slight differences between kidney and brain but my guess is that if there is a difference it is insignificant. Before trying to design (how are you going to define fixation?) and perform such investigations I suggest a trip to the library. I doubt if the information you seek is on line, it is too old. However, do not confuse "old" with "out dated" or "bad". Good luck. Geoff Karen_Skish@rush.edu wrote: > Hi-- > One of our investigators is interested in the approximate rate of fixation > of human brain tissue, independent of any formaldehyde diffusion effects. > In other words, in a very small or very thin piece of human brain tissue, > what is the fixation rate? He found published data for rat kidney, but > would like to try to at least determine if the fixation rate should be > higher or lower in human brain tissue. He is looking for data for room > temperature, but any information would be greatly appreciated. > Thanks! > Karen M Skish, MS, PA(ASCP)MT > Pathologists' Assistant & Manager, Neuropathology Lab > Rush Alzheimer's Disease Center > Cohn Research Building, Lab 441 > 1735 West Harrison Street > Chicago IL 60612 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** ------------------------------ Message: 19 Date: Wed, 28 May 2008 10:29:38 -0400 From: "Bryan Hewlett" Subject: Re: [Histonet] Rate of formalin penetration in human brain sections To: , Message-ID: <001301c8c0cf$430e19e0$6700a8c0@mainbox> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Karen, The formaldehyde fixation rate of tissue is determined from a combination of the penetration rate, which is governed by diffusibilty, the maximal covalent formaldehyde binding time, which is governed by the formaldehyde 'clock' reaction, and the slow subsequent cross-linking which then occurs, This slow cross-linking is thought to be almost complete by 7 days post formaldehyde binding time, but can continue over longer time periods. What your investigator has found, is probably the published data related to the maximal covalent formaldehyde binding time, i.e. 24 hours at room temperature as determined by Fox et al (reference #1). This study was essentially independent of diffusibilty, i.e. negligible penetration time, since 16 micrometer thick sections of rat kidney were the substrate. However, this study does not take into account subsequent further cross-linking. A study by Helander (reference #2) determined the maximal covalent formaldehyde binding time as 25 hours. This was performed on 4 mm thick slices of rabbit liver, so diffusibility has to be taken into account. In addition, this study also partially looked at subsequent further cross-linking in relationship to reversibility. A later study by Helander (reference #3) compared the maximal covalent formaldehyde binding time between kidney and brain tissues. This was stated to be 50 hours however, the tissues thickness was increased to 8 mm and so the diffusibilty effect has to be taken into account even more. Reference #1. Fox CH., et.al. Formaldehyde fixation. J Histochem. Cytochem. 1985; 33, 845 -853 Reference #2 Helander, KG. Kinetic studies of formaldehyde binding in tissue. Biotechnique and Histochemistry. 1994; 69, 177 -179 Reference #3 Helander, K.G. Formaldehyde binding in Brain and Kidney: A kinetic study of fixation. The Journal of Histotechnology. 1999; 22(4), 317-318. Regards, Bryan ----- Original Message ----- From: To: Sent: Tuesday, May 27, 2008 4:45 PM Subject: [Histonet] Rate of formalin penetration in human brain sections > Hi-- > One of our investigators is interested in the approximate rate of fixation > of human brain tissue, independent of any formaldehyde diffusion effects. > In other words, in a very small or very thin piece of human brain tissue, > what is the fixation rate? He found published data for rat kidney, but > would like to try to at least determine if the fixation rate should be > higher or lower in human brain tissue. He is looking for data for room > temperature, but any information would be greatly appreciated. > Thanks! > Karen M Skish, MS, PA(ASCP)MT > Pathologists' Assistant & Manager, Neuropathology Lab > Rush Alzheimer's Disease Center > Cohn Research Building, Lab 441 > 1735 West Harrison Street > Chicago IL 60612 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 20 Date: Wed, 28 May 2008 09:30:08 -0500 From: "Ingles Claire" Subject: [Histonet] NSH teleconference today To: Message-ID: <08A0A863637F1349BBFD83A96B27A50A120138@uwhis-xchng3.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="iso-8859-1" Help! I don't seem to have recieved the e-mail giving me the site to download the lecture materials. Anyone have it? Claire ------------------------------ Message: 21 Date: Wed, 28 May 2008 09:33:31 -0500 From: "Juan Gutierrez" Subject: RE: [Histonet] heat + formalin To: "'Nancy Schmitt'" , Message-ID: <004201c8c0cf$ce555040$6a00a8c0@precisionpath.lcl> Content-Type: text/plain; charset="US-ASCII" Hi Nancy, if you are doing breast prognostic markers on your cases, you might want to let it fix overnight before processing. The FDA requires it for Her-2 testing. No we do not use heat on our formalin steps, but we require our docs to cut tissue at 3mm or less thickness. So far this has been working fine with fixing and infiltration. Good luck, Juan C. Gutierrez, HT(ASCP) Precision Pathology Services 210.646.0890 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Wednesday, May 28, 2008 9:09 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] heat + formalin Good Morning Need some input, please. Does anyone combine heat during the formalin part of processing? We are having some issues with breast cases that need to be read out the next am - we are running them on a shorter processing schedule (done at 4 am) - and not having the best results.We use a closed system Tissue-Tek VIP 3000. Is anybody using microwave fixation? I think we will just put them on the longer processor (done at 0630) and be done - but wanted to check for other options. Thanks in advance Nancy Schmitt Histology Coordinator Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 22 Date: Wed, 28 May 2008 07:45:20 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] heat + formalin To: Nancy Schmitt , "'histonet@lists.utsouthwestern.edu'" Message-ID: <743811.30001.qm@web65704.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I never used heat with the formalin because fumes issues (if by any chance we had to open the retort). You should not run breast in short cycles unless the slices are extremely thin and you increase the time in the clearing agent (taking away from the alcohols). It is better to have a good section a few hours later, than a useless section rapidly. Ren? J. Nancy Schmitt wrote: Good Morning Need some input, please. Does anyone combine heat during the formalin part of processing? We are having some issues with breast cases that need to be read out the next am - we are running them on a shorter processing schedule (done at 4 am) - and not having the best results.We use a closed system Tissue-Tek VIP 3000. Is anybody using microwave fixation? I think we will just put them on the longer processor (done at 0630) and be done - but wanted to check for other options. Thanks in advance Nancy Schmitt Histology Coordinator Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 23 Date: Wed, 28 May 2008 11:12:43 -0400 From: stephanie.d.rivera@gsk.com Subject: [Histonet] Formalin alternative fixative for rodent and large animal To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Hello histoworld, Does anyone have any suggestions for altenative fixative to formalin? I still need to maintain quality of IHC and H&E stain and morphology. I've come across Prefer and Notoxhisto. Are there any others for animal tissue that anyone have tried? Stephanie D. Rivera Safety Assessment Department GlaxoSmithKline 709 Swedeland RD King of Prussia, PA 19406 phone: 610-270-7340 fax: 610-270-7202 ------------------------------ Message: 24 Date: Wed, 28 May 2008 11:20:18 -0400 From: "Nicole C Walsh" Subject: [Histonet] Experiencing Chatter in paraffin ribbons To: histonet@lists.utsouthwestern.edu Message-ID: <94A5E686-2355-422F-8F12-27F149E0EAB8@umassmed.edu> Content-Type: text/plain; charset=us-ascii; delsp=yes; format=flowed Hi, We routinely cut 5micron paraffin sections of decalcified mouse bones including whole hindpaws. We use Surgipath Infiltration medium for infiltration steps and then Surgipath EM-400 Embedding paraffin for embedding. This has worked well in the past, but since moving to a new institution and using different microtome setup we are now having problems with sectioning. Particularly we are experiencing a lot of chatter in the surrounding wax. We routinely keep the blocks cold when sectioning.. cooling in between each ribbon of 5-7 sections (maintained at 4degC on cooling block on embedding station, with slight coating of block softener (60% glycerol, 20% ethanol and 20% water)). The tissue generally comes off intact suggesting that infiltration of the tissue is reasonable. We are trying to eliminate possible reasons for our problems. A couple of things have changed since moving here.. We recently refreshed our paraffin supplies and this seems to have been when our troubles really started. But Surgipath have said that they have not changed the formula, but have given us paraffin from a different lot number to try. We've had a maintenance rep recently service the microtome (Microm HM315) and he could not find reason for the chatter (we section with angle set to 10 as this is what the rep suggested should be used for this microtome, and have tried varying the angle previously with no success) We used to use surgipath high profile blades (non-coated) with success (and I've seen discussion on histonet to say that the high profile blades are better for hard tissue). But the microtome that we now use will only take low-profile blades and so far we've tried the Richard Allan Scientific low profile blades (found that these became blunt quickly) and are now trying the teflon coated low profile blades from surgipath. If anyone has any suggestions for things that we might try that could help correct our problems we would be very grateful. Thanks in advance, Nicole ------------------------------ Message: 25 Date: Wed, 28 May 2008 10:28:51 -0500 From: "Mahoney,Janice A" Subject: [Histonet] RE: NSH teleconference today To: Ingles Claire , "histonet@lists.utsouthwestern.edu" Message-ID: <346E5878979BA54FB4B0BFD6AD93B9B9B01EE96359@EXCHMBC1.ad.ah.local> Content-Type: text/plain; charset="us-ascii" Me either!!!!! Janice Mahoney HT(ASCP) Histology/Cytology Coordinator Alegent Health Laboratory 4955 F Street Omaha, NE (402)717-2889 fax(402)717-5231 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire [CIngles@uwhealth.org] Sent: Wednesday, May 28, 2008 9:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NSH teleconference today Help! I don't seem to have recieved the e-mail giving me the site to download the lecture materials. Anyone have it? Claire _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ------------------------------ Message: 26 Date: Wed, 28 May 2008 16:31:11 +0100 From: "Marshall Terry Dr, Consultant Histopathologist" Subject: RE: [Histonet] Rate of formalin penetration in human brain sections To: "Geoff McAuliffe" , Cc: histonet@lists.utsouthwestern.edu Message-ID: <5C0BED61F529364E86309CADEA63FEF20163F40A@TRFT-EX01.xRothGen.nhs.uk> Content-Type: text/plain; charset="us-ascii" Agree with Geoff entirely. In particular you will not find it possible to define an end-point for formalin fixation. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff McAuliffe Sent: 28 May 2008 15:19 To: Karen_Skish@rush.edu Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Rate of formalin penetration in human brain sections Hi Karen: Formalin fixes tissue slowly, even very thin slices. This has been known for many, many years. The work of Medwar and of John R. Baker (Principles of Biological Technique, John Wiley and Sons, 1958) comes to mind. Perhaps there are slight differences between kidney and brain but my guess is that if there is a difference it is insignificant. Before trying to design (how are you going to define fixation?) and perform such investigations I suggest a trip to the library. I doubt if the information you seek is on line, it is too old. However, do not confuse "old" with "out dated" or "bad". Good luck. Geoff Karen_Skish@rush.edu wrote: > Hi-- > One of our investigators is interested in the approximate rate of > fixation of human brain tissue, independent of any formaldehyde diffusion effects. > In other words, in a very small or very thin piece of human brain > tissue, what is the fixation rate? He found published data for rat > kidney, but would like to try to at least determine if the fixation > rate should be higher or lower in human brain tissue. He is looking > for data for room temperature, but any information would be greatly appreciated. > Thanks! > Karen M Skish, MS, PA(ASCP)MT > Pathologists' Assistant & Manager, Neuropathology Lab Rush Alzheimer's > Disease Center Cohn Research Building, Lab 441 > 1735 West Harrison Street > Chicago IL 60612 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 54, Issue 41 **************************************** From pmarcum <@t> vet.upenn.edu Wed May 28 10:49:15 2008 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Wed May 28 10:49:24 2008 Subject: [Histonet] Formalin alternative fixative for rodent and large animal In-Reply-To: References: Message-ID: <6.2.5.6.2.20080528114231.01c8c4e8@vet.upenn.edu> I agree with Jackie and remember most antibodies are worked out for use with formalin not a substitute. You will need to re-validate and test every primary and secondary antibody as well as kits you have used to be sure you are getting the same results with the new fixative. You will need to run parallel tissues and times for processing to confirm the change works for specimens in you laboratory. Until the antibody and kit or secondary suppliers begin to use a formalin replacement most testing for routine use and research is on you not the company. Be careful to cover all your bases for the best results in tissue staining for both routine and IHC. Pam Marcum At 11:32 AM 5/28/2008, Jackie M O'Connor wrote: >In my opinion, for pharma research, there is no worthwhile formalin >substitute that will give consistent IHC results. I have tried STF >(which was discontinued) and other subs - finally went back to formalin. >With STF, the morphology of routine H+E's was horrible. I'm sure there >are differing opinions out there - after all, this is histonet. > >Jackie O' > > > >stephanie.d.rivera@gsk.com >Sent by: histonet-bounces@lists.utsouthwestern.edu >05/28/2008 10:12 AM > >To >histonet@lists.utsouthwestern.edu >cc > >Subject >[Histonet] Formalin alternative fixative for rodent and large animal > > > > > > >Hello histoworld, > >Does anyone have any suggestions for altenative fixative to formalin? I >still need to maintain quality of IHC and H&E stain and morphology. I've >come across Prefer and Notoxhisto. Are there any others for animal tissue >that anyone have tried? > > > > > > >Stephanie D. Rivera >Safety Assessment Department >GlaxoSmithKline >709 Swedeland RD >King of Prussia, PA 19406 >phone: 610-270-7340 >fax: 610-270-7202 >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From ryaskovich <@t> dir.nidcr.nih.gov Wed May 28 10:55:51 2008 From: ryaskovich <@t> dir.nidcr.nih.gov (Yaskovich, Ruth A (NIH/NIDCR) [E]) Date: Wed May 28 10:56:53 2008 Subject: [Histonet] Formalin alternative fixative for rodent andlarge animal In-Reply-To: <6.2.5.6.2.20080528114231.01c8c4e8@vet.upenn.edu> References: <6.2.5.6.2.20080528114231.01c8c4e8@vet.upenn.edu> Message-ID: I have been getting my best results with 4% BUFFERED PFA. The animals are perfused. I'm doing CNS tissues with beautiful IHC. Ruth Yaskovich N.I.H. -----Original Message----- From: Pamela Marcum [mailto:pmarcum@vet.upenn.edu] Sent: Wednesday, May 28, 2008 11:49 AM To: Jackie M O'Connor; stephanie.d.rivera@gsk.com Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Formalin alternative fixative for rodent andlarge animal I agree with Jackie and remember most antibodies are worked out for use with formalin not a substitute. You will need to re-validate and test every primary and secondary antibody as well as kits you have used to be sure you are getting the same results with the new fixative. You will need to run parallel tissues and times for processing to confirm the change works for specimens in you laboratory. Until the antibody and kit or secondary suppliers begin to use a formalin replacement most testing for routine use and research is on you not the company. Be careful to cover all your bases for the best results in tissue staining for both routine and IHC. Pam Marcum At 11:32 AM 5/28/2008, Jackie M O'Connor wrote: >In my opinion, for pharma research, there is no worthwhile formalin >substitute that will give consistent IHC results. I have tried STF >(which was discontinued) and other subs - finally went back to formalin. >With STF, the morphology of routine H+E's was horrible. I'm sure there >are differing opinions out there - after all, this is histonet. > >Jackie O' > > > >stephanie.d.rivera@gsk.com >Sent by: histonet-bounces@lists.utsouthwestern.edu >05/28/2008 10:12 AM > >To >histonet@lists.utsouthwestern.edu >cc > >Subject >[Histonet] Formalin alternative fixative for rodent and large animal > > > > > > >Hello histoworld, > >Does anyone have any suggestions for altenative fixative to formalin? I >still need to maintain quality of IHC and H&E stain and morphology. I've >come across Prefer and Notoxhisto. Are there any others for animal tissue >that anyone have tried? > > > > > > >Stephanie D. Rivera >Safety Assessment Department >GlaxoSmithKline >709 Swedeland RD >King of Prussia, PA 19406 >phone: 610-270-7340 >fax: 610-270-7202 >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Wed May 28 11:28:29 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed May 28 11:28:49 2008 Subject: [Histonet] My last hope for FFPE anti rat CD4 In-Reply-To: Message-ID: This topic has been beaten to death - but I'll ask again just incase there has been a miracle breakthrough. Anyone know of an anti-rat CD4 antibody that will work in FFPE? Don't yell at me, please. Thanks, Jackie O' From gayle.callis <@t> bresnan.net Wed May 28 11:40:38 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Wed May 28 11:40:48 2008 Subject: Discussion on formalin Re: [Histonet] Rate of formalin penetration in human brain sections References: <5C0BED61F529364E86309CADEA63FEF20163F40A@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <001001c8c0e1$8f2acaa0$6401a8c0@DHXTS541> One of the best discussions on formalin and how fast it fixes/penetrates can be found on Histonet with Bryan Hewlett. Go back in the archives, as this subject has been visited before. Look for what Bryan has to say about formalin fixation. His talk at NSH convention last year (IHC forum) was one of the most enlightening I have ever heard - even after over 40 years in this business. He emphasized how long it takes to achieve total fixation of a tissue submitted for processing in terms of days and hours. Hopefully he is looking in on this discussion. There are also journal publications on the subject, Biotechnics and Histochemistry, Journal of Histotechnology - try Google Scholar, Biosis, Scientific Citations. I wish I still had the Baker book, but alas, gave it to a histobook collector. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT ----- Original Message ----- From: "Marshall Terry Dr,Consultant Histopathologist" To: "Geoff McAuliffe" ; Cc: Sent: Wednesday, May 28, 2008 9:31 AM Subject: RE: [Histonet] Rate of formalin penetration in human brain sections Agree with Geoff entirely. In particular you will not find it possible to define an end-point for formalin fixation. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff McAuliffe Sent: 28 May 2008 15:19 To: Karen_Skish@rush.edu Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Rate of formalin penetration in human brain sections Hi Karen: Formalin fixes tissue slowly, even very thin slices. This has been known for many, many years. The work of Medwar and of John R. Baker (Principles of Biological Technique, John Wiley and Sons, 1958) comes to mind. Perhaps there are slight differences between kidney and brain but my guess is that if there is a difference it is insignificant. Before trying to design (how are you going to define fixation?) and perform such investigations I suggest a trip to the library. I doubt if the information you seek is on line, it is too old. However, do not confuse "old" with "out dated" or "bad". Good luck. Geoff Karen_Skish@rush.edu wrote: > Hi-- > One of our investigators is interested in the approximate rate of > fixation of human brain tissue, independent of any formaldehyde diffusion effects. > In other words, in a very small or very thin piece of human brain > tissue, what is the fixation rate? He found published data for rat > kidney, but would like to try to at least determine if the fixation > rate should be higher or lower in human brain tissue. He is looking > for data for room temperature, but any information would be greatly appreciated. > Thanks! > Karen M Skish, MS, PA(ASCP)MT > Pathologists' Assistant & Manager, Neuropathology Lab Rush Alzheimer's > Disease Center Cohn Research Building, Lab 441 > 1735 West Harrison Street > Chicago IL 60612 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BrealK <@t> alexian.net Wed May 28 12:03:39 2008 From: BrealK <@t> alexian.net (Kari Breal) Date: Wed May 28 12:03:56 2008 Subject: [Histonet] Histology position Message-ID: <20080528T120339Z_439500110000@alexian.net> Hello Histonetters! Alexian Brothers Medical Center in Elk Grove IL (Chicago NW Suburb) has an open Histologist position. The hours are 5:30 to 2pm. Please contact me if interested. Thanks Kari Breal Histology Supervisor Alexian Brothers Medical Center 847-437-5500 ext. 5155 Fax 847-981-2023 brealk@alexian.netCONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From histonet <@t> metasynthesis.net Wed May 28 12:07:24 2008 From: histonet <@t> metasynthesis.net (William Gunn) Date: Wed May 28 12:07:29 2008 Subject: [Histonet] Contract histomorphometry and stereology Message-ID: <73b82faa0805281007g33504f79pd10612338aa114a1@mail.gmail.com> Hi Histonetters! I'm looking for a lab or service that can perform bone histomorphometry on FFPE, EDTA-decalcified, whole mouse legs. The legs are intact, with tissue attached, from the distal end of the tibia to the proximal end of the femur, and I need a measurement of trabecular volume on about 40 sections. If possible, I'd also like some other bone pathology-related parameters, which we can discuss further. Rapid turn-around time would be a bonus. Does anyone know who I should contact? From making <@t> ufl.edu Wed May 28 12:09:33 2008 From: making <@t> ufl.edu (MKing) Date: Wed May 28 12:11:51 2008 Subject: [Histonet] Thick section penetration for IHC Message-ID: <483D91CD.2000001@ufl.edu> Bob, There's always fluorescence--these authors used 100 um Vibratome sections and found that the primary antibody may make a difference too. Journal of Neuroscience Methods 170 (2008) 165?178 Associative image analysis: A method for automated quantification of 3D multi-parameter images of brain tissue Christopher S. Bjornsson, Gang Lin, Yousef Al-Kofahi, Arunachalam Narayanaswamy, Karen L. Smith, William Shain, Badrinath Roysamb Simple avidinylated HRP may be smaller than ABC complexes and penetrate better. People have also used DMSO 1-10% in incubation solutions, and ethanol extraction steps on cut thick sections, reported to improve penetration (both in histonet archives). Good histo, Mike King UF Pharmacology & Therapeutics -------------- Message: 3 Date: Tue, 27 May 2008 10:04:52 -0800 From: "Bob Nienhuis" Subject: [Histonet] Thick section penetration for IHC To: Histonet It has been suggested that the ABC detection system may not be the optimal choice for IHC in thick brain sections (~100 microns). Apparently, the large avidin-biotin complexes may not penetrate tissue too well. What would you suggest instead, LSAB, polymer or...? Bob Nienhuis Neurobiology Research UCLA / VA Medical Center Los Angeles -------------- next part -------------- Bjornsson CS, Lin G, Al-Kofahi Y, Narayanaswamy A, Smith KL, Shain W, Roysam B. Associative image analysis: A method for automated quantification of 3D multi-parameter images of brain tissue. J Neurosci Methods. 2008 May 15;170(1):165-78. use 100 um sections, get complete penetration with some Abs, not GFAP, ------------------ Date: 15 Feb 1999 18:30:55 -0600 From: larisonk@UONEURO.uoregon.edu Subject: Re: 100 micron floating sections Nancy, When we stain whole mount embryos, we find that adding 1% DMSO to all incubation and wash buffers enhances staining. In fact, with the older embryos, pre-incubating them overnight in buffer containing 10% DMSO seems to help a great deal. Wash thourougly before adding your primary, however. 1% DMSO doesn't seem to affect antibody binding, whereas higher concentrations apparently does. In most cases, we also find that immersing the tissue briefly (7 minutes) in ice-cold (-20C) acetone before the blocking step also helps. We also use triton and tween. The detergents, however, are harder on the tissue than DMSO. Tween may be a bit gentler, and with some antibodies appears to be more effective. Also, we usually incubate all reagents for at least 5 h or overnight, and wash several times over the course of two hours. Also, all antibodies do not penetrate equally well. Some antibodies that work well on sections, simply will not stain in whole mounts. Probably it has to do with the charge of the antibody or the location of the antigen. Also, I find that the fluorescent tyramide HRP substrates provide a nice consistent signal throughout the tissue, whereas fluorescent secondaries or HRP/DAB staining sometimes results in inconsistent or spotty staining. Good luck. Karen Larison - University of Oregon ---------------------------- Date: 15 Feb 1999 19:00:14 -0600 From: klosen@neurochem.u-strasbg.fr (Paul Klosen) Subject: Re: 100 micron floating sections >Does anyone have any sure fire tips for immunostaining 100 micron floating >sections so that the antibodies actually penetrate? Triton, >saponin...whatever? Any comments or suggestions will be appreciated. >Thanks Nancy Lemke Try the buffered ethanol procedure. Prepare 10%, 25% and 40% ethanol buffered with 100 mM phosphate at pH 7,4. 40% buffered ethanol needs quite some adjustments. Take the sections up and down these ethanols for 5 to 10 min each. I have yet to see an antibody that does no longer label after this pretreatment. And the penetration always worked fine. According to some authors (I borrewed their procedure, but have to look up the precise ref), even the ultrastructure is well conserved, although not as well as if you avoid permeabilisation. If you plan on doing preembedding EM ICC, be aware that no procedure that allows good penetration throughout thick sections will give you a perfect ultrastructure. One further tip. Do not use ABC complexes !!! These are quite big and will never well penetrate. Use either streptavidin/avidin conjugates (Perox directly linked to streptavidin, the Boehringer reagent does wonders) or build the complexes in situ by incubating first with the streptavidin and then the biotinylated label. The use of streptavin conjugates also works much better if you use Triton or Saponin permeabilisation. From JMaslanka <@t> stpetes.org Wed May 28 12:26:48 2008 From: JMaslanka <@t> stpetes.org (JMaslanka@stpetes.org) Date: Wed May 28 12:28:10 2008 Subject: [Histonet] ?Quicker Copper Stain Message-ID: Hi, does anyone have a quicker procedure for staining copper in liver other than the overnight method? If so would you please share. Thanks Joe Maslanka Cyto/Histo Coord. St Peter's Laboratory " Not everything that can be counted counts..... Not everything that counts can be counted." Albert Einstein From PMonfils <@t> Lifespan.org Wed May 28 12:40:17 2008 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed May 28 12:40:26 2008 Subject: [Histonet] flattening glycol methacrylate sections Message-ID: <4EBFF65383B74D49995298C4976D1D5E273D87@LSRIEXCH1.lsmaster.lifespan.org> Do you float your G.M. sections on a water bath? If so, what temperature? Do you add anything to the water? From HornHV <@t> archildrens.org Wed May 28 12:43:43 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed May 28 12:43:44 2008 Subject: [Histonet] ?Quicker Copper Stain In-Reply-To: References: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82C84@EMAIL.archildrens.org> American Master Tech Scientific has a microwave copper stain kit. It works very well and is FAST! Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JMaslanka@stpetes.org Sent: Wednesday, May 28, 2008 12:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ?Quicker Copper Stain Hi, does anyone have a quicker procedure for staining copper in liver other than the overnight method? If so would you please share. Thanks Joe Maslanka Cyto/Histo Coord. St Peter's Laboratory " Not everything that can be counted counts..... Not everything that counts can be counted." Albert Einstein _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From contact <@t> excaliburpathology.com Wed May 28 12:54:51 2008 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Wed May 28 12:54:55 2008 Subject: [Histonet] flattening glycol methacrylate sections Message-ID: <815024.40176.qm@web50107.mail.re2.yahoo.com> Try?room temp. water with a few drops of ammonium hydroxide. ?Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 631 N. Broadway Ave. Moore, OK 73160 405-570-6679 cell 405-759-3953 lab contact@excaliburpathology.com www.excaliburpathology.com From jjenkins <@t> sch-farmville.org Wed May 28 12:50:55 2008 From: jjenkins <@t> sch-farmville.org (Jennie Jenkins) Date: Wed May 28 12:56:56 2008 Subject: [Histonet] MLT working histology? Message-ID: <483D9B7F.603@sch-farmville.org> Can an MLT work in histology? She would be embedding, cutting, staining, cover slipping, labeling, filing, requisitioning and setting up the gross. If possible we could have her trained to gross in anything that would not require "cutting", like GI biopsies. Jennie Jenkins, PA(ASCP) Southside Community Hospital 800 Oak Street Farmville, Virginia 23901 ================================================== This e-mail message (and attachments) may contain information that is confidential to Southside Community Hospital. If you are not the intended recipient you cannot use, distribute or copy the message or attachments. In such a case, please notify the sender by return e-mail immediately and erase all copies of the message and attachments. Opinions, conclusions and other information in this message and attachments that do not relate to the official business of Southside Community Hospital are neither given nor endorsed by it. ================================================== From sbreeden <@t> nmda.nmsu.edu Wed May 28 13:32:16 2008 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed May 28 13:32:20 2008 Subject: [Histonet] Shelf Life Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E6349@nmdamailsvr.nmda.ad.nmsu.edu> Is there a comprehensive chart somewhere that refers to shelf life for (in-house) prepared stains, i.e., Toluidine Blue, Light Green SF Yellowish? I have found a nice chart in Frieda Carson's "Histotechnology: A Self- Instructional Text" but it is not inclusive. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From jkiernan <@t> uwo.ca Wed May 28 13:43:15 2008 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed May 28 13:43:22 2008 Subject: [Histonet] ?Quicker Copper Stain In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82C84@EMAIL.archildrens.org> References: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82C84@EMAIL.archildrens.org> Message-ID: If you are staining for copper in sections of a Wilson reagent is a temperature and p-dimethylaminobenzylidenerhodanine, 37C. For a comparison see Irons et al. 1977 Path. Lab. Med. 101& nbsp;
John Kiernan
Anatomy, UWO
London, Canad -----
From: <HornHV@archildrens.org>
Wednesday, May 28, 2008 13:44
Subject: RE: [H istonet] ?Quicker Copper Stain
To: JMaslanka@stpetes.or histonet@lists.utsouthwestern.edu

> Ame rican Master Tech Scientific has a microwave copper stain
> very well and is FA Horn
> Hazel Ho Supervisor of Histology
> Hospital
> 800 Marshall  Slot 820
> Little Rock, AR  72202
>
> phone   501.364.4240
> fax   & nbsp;    501.364.3155
> < at:    ww w.archildrens.org
>
> -----Original Mes histonet-bounces@lists.utsouthwest [mailto:histonet-bounces@lists.utsouth Behalf Of
> JMaslanka@stpetes.org< Sent: Wednesday, May 28, 2008 12:27 PM
&g histonet@lists.utsouthwestern.edu
> Subje [Histonet] ?Quicker Copper Stain
>
& gt; Hi, does anyone have a quicker procedure for staining copper in method? Thanks
> Cyto/Histo Coord.
Laboratory
>
> " No can be counted counts.....
> & everything that counts can be counted."
>         & ________ _______________________ 5F Histonet m Histonet@lists.utsouthwestern.edu
>
> *********************************** ********************************************************************** ** ********************************************************************** ** ********************************************************************** ** ********************************************************************** ** ********************************************************************** ** ********************************************************************** ** ********************************************************************** ** ********************************************************************** ** this me protected f message is not employee or agent respo
> message to notified that
> distribution or copying of this co strictly
> prohibited. communication in error,
> ple
> us immediately by replying to the message an deleting it from
> your computer.
> Tha __________ _______________________ 5F mailing Histonet@lists.utsouthwestern.edu
& http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Wed May 28 13:42:46 2008 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Wed May 28 13:45:21 2008 Subject: [Histonet] Formalin alternative fixative for rodent and large animal In-Reply-To: References: Message-ID: <898925661-1212000309-cardhu_decombobulator_blackberry.rim.net-1022827500-@bxe115.bisx.prod.on.blackberry> I do a wide range of IHC and H+E on rodent tissue routinely using 4% PFA. -----Original Message----- From: stephanie.d.rivera@gsk.com Date: Wed, 28 May 2008 11:12:43 To:histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin alternative fixative for rodent and large animal Hello histoworld, Does anyone have any suggestions for altenative fixative to formalin? I still need to maintain quality of IHC and H&E stain and morphology. I've come across Prefer and Notoxhisto. Are there any others for animal tissue that anyone have tried? Stephanie D. Rivera Safety Assessment Department GlaxoSmithKline 709 Swedeland RD King of Prussia, PA 19406 phone: 610-270-7340 fax: 610-270-7202 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Wed May 28 13:54:57 2008 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed May 28 13:55:01 2008 Subject: [Histonet] Shelf Life Message-ID: There is a pretty comprehensive list in "Manual of the Special Stains Laboratory" by Charles J. Churukian. This manual is published by the Pathology Department at the University of Rochester Medical Center, Rochester, NY. ----- Original Message ----- From: "Breeden, Sara" Date: Wednesday, May 28, 2008 14:34 Subject: [Histonet] Shelf Life To: histonet@lists.utsouthwestern.edu > Is there a comprehensive chart somewhere that refers to shelf > life for > (in-house) prepared stains, i.e., Toluidine Blue, Light Green SF > Yellowish? I have found a nice chart in Frieda Carson's > "Histotechnology: A Self- Instructional Text" but it is not inclusive. > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Wed May 28 13:58:00 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Wed May 28 13:58:08 2008 Subject: [Histonet] Shelf Life References: <4D14F0FC9316DD41972D5F03C070908B017E6349@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <008701c8c0f4$bfe70240$6401a8c0@DHXTS541> Try HistoLogic from Sakura Finetek. There was a list created a long time ago. All published Histologics are available on their website. Good luck Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT ----- Original Message ----- From: "Breeden, Sara" To: Sent: Wednesday, May 28, 2008 12:32 PM Subject: [Histonet] Shelf Life Is there a comprehensive chart somewhere that refers to shelf life for (in-house) prepared stains, i.e., Toluidine Blue, Light Green SF Yellowish? I have found a nice chart in Frieda Carson's "Histotechnology: A Self- Instructional Text" but it is not inclusive. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Wed May 28 14:01:08 2008 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed May 28 14:01:12 2008 Subject: [Histonet] Shelf Life In-Reply-To: <008701c8c0f4$bfe70240$6401a8c0@DHXTS541> References: <4D14F0FC9316DD41972D5F03C070908B017E6349@nmdamailsvr.nmda.ad.nmsu.edu> <008701c8c0f4$bfe70240$6401a8c0@DHXTS541> Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E634A@nmdamailsvr.nmda.ad.nmsu.edu> Thank you, Gayle - will do! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 -----Original Message----- From: Gayle Callis [mailto:gayle.callis@bresnan.net] Sent: Wednesday, May 28, 2008 12:58 PM To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Shelf Life Try HistoLogic from Sakura Finetek. There was a list created a long time ago. All published Histologics are available on their website. Good luck Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT ----- Original Message ----- From: "Breeden, Sara" To: Sent: Wednesday, May 28, 2008 12:32 PM Subject: [Histonet] Shelf Life Is there a comprehensive chart somewhere that refers to shelf life for (in-house) prepared stains, i.e., Toluidine Blue, Light Green SF Yellowish? I have found a nice chart in Frieda Carson's "Histotechnology: A Self- Instructional Text" but it is not inclusive. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From renafail <@t> bellsouth.net Wed May 28 14:06:07 2008 From: renafail <@t> bellsouth.net (Rena Fail) Date: Wed May 28 14:06:08 2008 Subject: [Histonet] ?Quicker Copper Stain In-Reply-To: Message-ID: <000c01c8d951$ecd2b770$0301a8c0@RENAD4YK9B8ABE> Place your working rhodanine solution in a 60 degree water bath for 10 minutes to preheat then place your slides in the solution at 60 degrees for 10 minutes Rena Fail -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JMaslanka@stpetes.org Sent: Wednesday, May 28, 2008 1:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ?Quicker Copper Stain Hi, does anyone have a quicker procedure for staining copper in liver other than the overnight method? If so would you please share. Thanks Joe Maslanka Cyto/Histo Coord. St Peter's Laboratory " Not everything that can be counted counts..... Not everything that counts can be counted." Albert Einstein _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Wed May 28 14:08:18 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Wed May 28 14:08:22 2008 Subject: [Histonet] flattening glycol methacrylate sections References: <815024.40176.qm@web50107.mail.re2.yahoo.com> Message-ID: <008d01c8c0f6$304b2ce0$6401a8c0@DHXTS541> We never used warm water baths since the fumes from glycol methacrylate are toxic, plus one can become very sensitized to this plastic. We used a cold water bath, a large square glass staining dish either 20 or 30 slide capacity size, lifted the section from the glass knife edge with a sharp forceps, and dropped it onto RT water. It takes a bit of skill to do this and if helped to have a magnifier lamp during sectioning. When we used ammonia drops in the water, our sections took a dive to bottom of staining dish (square), probably more of a technic issue. You can still try the ammonia hydroxide drops though. Using a very sharp, new edge was mandatory with glass knives to get a flat section to begin with. If your knife is not delightfully sharp, this will contribute to sections not being flat. Tungsten carbide knives need to be kept very sharp also. We changed the glass knife every time we cut a new block, but did use the old knife to trim before sectioning with the new glass knife. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT ----- Original Message ----- From: "Paula Pierce" To: "Histonet" Sent: Wednesday, May 28, 2008 11:54 AM Subject: [Histonet] flattening glycol methacrylate sections Try room temp. water with a few drops of ammonium hydroxide. Paula Pierce, HTL(ASCP)HT From sbreeden <@t> nmda.nmsu.edu Wed May 28 14:20:59 2008 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed May 28 14:21:04 2008 Subject: [Histonet] Shelf Life, Part II Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E634D@nmdamailsvr.nmda.ad.nmsu.edu> Way in the back of my gray matter (brain, not hair...) comes a little memory: "stains can be reused, solutions not". I take/took this to mean that stains like carbol fuchsin could be reused (after filtering) but that a 1% acetic acid could not. How confused am I (need I ask?)? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From akemiat3377 <@t> yahoo.com Wed May 28 14:25:32 2008 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Wed May 28 14:25:46 2008 Subject: [Histonet] flattening glycol methacrylate sections In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E273D87@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <978771.8553.qm@web31307.mail.mud.yahoo.com> Way back when I was doing GMA (back in the late 70's and 80's) I placed my section on a DI water bath with NO heat, picked it up with my slide, then placed it on a slide warmer at-least 37 degrees centigrade or higher to adhere the section to the slide. Make sure you wipe the excess water from the back of the slide, so it doesn't snap crackle pop! Akemi Allison-Tacha, BS, HT(ASCP)HTL Client Services Manager PhenoPath laboratories 551 North 34th Street, Suite 100 Seattle, WA 98103-8675 Work: (206) 374-9000 ext 1053 E-Mail: akemiat3377@yahoo.com --- On Wed, 5/28/08, Monfils, Paul wrote: > From: Monfils, Paul > Subject: [Histonet] flattening glycol methacrylate sections > To: histonet@lists.utsouthwestern.edu > Date: Wednesday, May 28, 2008, 10:40 AM > Do you float your G.M. sections on a water bath? If so, > what temperature? Do you add anything to the water? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Wed May 28 14:26:24 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Wed May 28 14:26:28 2008 Subject: [Histonet] Shelf Life References: Message-ID: <00c101c8c0f8$b7e7e6f0$6401a8c0@DHXTS541> Here are the particulars for ordering Manual of the Special Stains Laboratory" by Charles J. Churukian Price: $45.00 (includes shipping) Send number of copies requested, shipping address, and billing address if it's different, to: Sandra Piampiano, Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, 601 Elmwood Avenue Box 626, Rochester, NY 14642. Or send an e-mail order to Mrs. Piampiano at Sandy_Piampiano@urmc.rochester.edu. Please note that we cannot accept credit cards, but can accept a purchase order number or billing address. Gayle M. Callis ----- Original Message ----- From: "John Kiernan" To: "Breeden, Sara" Cc: Sent: Wednesday, May 28, 2008 12:54 PM Subject: Re: [Histonet] Shelf Life > There is a pretty comprehensive list in "Manual of the Special Stains > Laboratory" by Charles J. Churukian. This manual is published by the > Pathology Department at the University of Rochester Medical Center, > Rochester, NY. > > From rjbuesa <@t> yahoo.com Wed May 28 14:36:02 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 28 14:36:09 2008 Subject: [Histonet] ?Quicker Copper Stain In-Reply-To: Message-ID: <779866.91803.qm@web65708.mail.ac4.yahoo.com> Tim's procedura (which for me has resulted the best) takes only 10 minutes to complete. Ren? J. JMaslanka@stpetes.org wrote: Hi, does anyone have a quicker procedure for staining copper in liver other than the overnight method? If so would you please share. Thanks Joe Maslanka Cyto/Histo Coord. St Peter's Laboratory " Not everything that can be counted counts..... Not everything that counts can be counted." Albert Einstein _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed May 28 14:38:27 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 28 14:38:32 2008 Subject: [Histonet] MLT working histology? In-Reply-To: <483D9B7F.603@sch-farmville.org> Message-ID: <25394.43930.qm@web65705.mail.ac4.yahoo.com> It depends on your State regulations, but from the general training point of view I think she can. Ren? J. Jennie Jenkins wrote: Can an MLT work in histology? She would be embedding, cutting, staining, cover slipping, labeling, filing, requisitioning and setting up the gross. If possible we could have her trained to gross in anything that would not require "cutting", like GI biopsies. Jennie Jenkins, PA(ASCP) Southside Community Hospital 800 Oak Street Farmville, Virginia 23901 ================================================== This e-mail message (and attachments) may contain information that is confidential to Southside Community Hospital. If you are not the intended recipient you cannot use, distribute or copy the message or attachments. In such a case, please notify the sender by return e-mail immediately and erase all copies of the message and attachments. Opinions, conclusions and other information in this message and attachments that do not relate to the official business of Southside Community Hospital are neither given nor endorsed by it. ================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed May 28 16:12:56 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 28 16:13:04 2008 Subject: [Histonet] Shelf Life, Part II In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E634D@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <860037.35512.qm@web65712.mail.ac4.yahoo.com> If you use the staining solution it will start to weaken by the distilled water you always carry with the slides that are going to be stained, so if you keep using it the solution will end weaker and with less staining "power". When they were only few slides I solved this problem by staining the slides on a rack and pouring the staining solution with a pipet over them. In that way the staining solution was used only once, and was not diluted (=weaken). Either staining solutions or solutions of any substance will not weaken if the slides arte not placed in them. Ren? J. "Breeden, Sara" wrote: Way in the back of my gray matter (brain, not hair...) comes a little memory: "stains can be reused, solutions not". I take/took this to mean that stains like carbol fuchsin could be reused (after filtering) but that a 1% acetic acid could not. How confused am I (need I ask?)? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Wed May 28 17:42:14 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Wed May 28 17:42:46 2008 Subject: [Histonet] Rate of formalin penetration in human brain sections References: <001301c8c0cf$430e19e0$6700a8c0@mainbox> Message-ID: <08A0A863637F1349BBFD83A96B27A50A12013B@uwhis-xchng3.uwhis.hosp.wisc.edu> Stop, my brain hurts! Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bryan Hewlett Sent: Wed 5/28/2008 9:29 AM To: histonet@lists.utsouthwestern.edu; Karen_Skish@rush.edu Subject: Re: [Histonet] Rate of formalin penetration in human brain sections Karen, The formaldehyde fixation rate of tissue is determined from a combination of the penetration rate, which is governed by diffusibilty, the maximal covalent formaldehyde binding time, which is governed by the formaldehyde 'clock' reaction, and the slow subsequent cross-linking which then occurs, This slow cross-linking is thought to be almost complete by 7 days post formaldehyde binding time, but can continue over longer time periods. What your investigator has found, is probably the published data related to the maximal covalent formaldehyde binding time, i.e. 24 hours at room temperature as determined by Fox et al (reference #1). This study was essentially independent of diffusibilty, i.e. negligible penetration time, since 16 micrometer thick sections of rat kidney were the substrate. However, this study does not take into account subsequent further cross-linking. A study by Helander (reference #2) determined the maximal covalent formaldehyde binding time as 25 hours. This was performed on 4 mm thick slices of rabbit liver, so diffusibility has to be taken into account. In addition, this study also partially looked at subsequent further cross-linking in relationship to reversibility. A later study by Helander (reference #3) compared the maximal covalent formaldehyde binding time between kidney and brain tissues. This was stated to be 50 hours however, the tissues thickness was increased to 8 mm and so the diffusibilty effect has to be taken into account even more. Reference #1. Fox CH., et.al. Formaldehyde fixation. J Histochem. Cytochem. 1985; 33, 845 -853 Reference #2 Helander, KG. Kinetic studies of formaldehyde binding in tissue. Biotechnique and Histochemistry. 1994; 69, 177 -179 Reference #3 Helander, K.G. Formaldehyde binding in Brain and Kidney: A kinetic study of fixation. The Journal of Histotechnology. 1999; 22(4), 317-318. Regards, Bryan ----- Original Message ----- From: To: Sent: Tuesday, May 27, 2008 4:45 PM Subject: [Histonet] Rate of formalin penetration in human brain sections > Hi-- > One of our investigators is interested in the approximate rate of fixation > of human brain tissue, independent of any formaldehyde diffusion effects. > In other words, in a very small or very thin piece of human brain tissue, > what is the fixation rate? He found published data for rat kidney, but > would like to try to at least determine if the fixation rate should be > higher or lower in human brain tissue. He is looking for data for room > temperature, but any information would be greatly appreciated. > Thanks! > Karen M Skish, MS, PA(ASCP)MT > Pathologists' Assistant & Manager, Neuropathology Lab > Rush Alzheimer's Disease Center > Cohn Research Building, Lab 441 > 1735 West Harrison Street > Chicago IL 60612 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MichelM9 <@t> chw.edu.au Wed May 28 17:58:23 2008 From: MichelM9 <@t> chw.edu.au (Michelle McDonald) Date: Wed May 28 17:58:34 2008 Subject: [Histonet] flattening glycol methacrylate sections In-Reply-To: <978771.8553.qm@web31307.mail.mud.yahoo.com> Message-ID: <47BD6E7614A693499453835D47E36F7004744C50@hedwig.nch.kids> For methyl methacrylate (MMA) sections we use 70% ethanol to lubricate a tungsten carbide blade and then slide the sections onto 70% ethanol dipped slides and sit them covered in 70% ethanol until ready to spread. Then we vertically dip the sections on the slides in an mixture of ethanol and ethylene glycol monoethyl ether warmed to 55 degrees C a few times to drag the wrinkles out and firmly place a piece of plastic on to the section to push out remaining wrinkles. We place 8 to 10 slides together with filter paper between and bulldog clip them together to apply pressure over night as they are baked at 50 degrees to adhere them. If you have a nice sharp blade and well processed blocks this should produce pretty flat sections. Although MMA sections are rarely as good as paraffin. If anyone would like I can email you our protocols directly for this if it helps Michelle Dr Michelle McDonald B.MedSci, PhD Research Officer Orthopaedic Research Dept. The Children's Hospital Westmead. Westmead NSW 2145 Australia Ph. +612 9845 1451 Fax. +612 9845 3078 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Thursday, 29 May 2008 5:26 AM To: histonet@lists.utsouthwestern.edu; Monfils, Paul Subject: Re: [Histonet] flattening glycol methacrylate sections Way back when I was doing GMA (back in the late 70's and 80's) I placed my section on a DI water bath with NO heat, picked it up with my slide, then placed it on a slide warmer at-least 37 degrees centigrade or higher to adhere the section to the slide. Make sure you wipe the excess water from the back of the slide, so it doesn't snap crackle pop! Akemi Allison-Tacha, BS, HT(ASCP)HTL Client Services Manager PhenoPath laboratories 551 North 34th Street, Suite 100 Seattle, WA 98103-8675 Work: (206) 374-9000 ext 1053 E-Mail: akemiat3377@yahoo.com --- On Wed, 5/28/08, Monfils, Paul wrote: > From: Monfils, Paul > Subject: [Histonet] flattening glycol methacrylate sections > To: histonet@lists.utsouthwestern.edu > Date: Wednesday, May 28, 2008, 10:40 AM > Do you float your G.M. sections on a water bath? If so, > what temperature? Do you add anything to the water? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From jnocito <@t> satx.rr.com Wed May 28 18:32:19 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed May 28 18:32:11 2008 Subject: [Histonet] RE: NSH teleconference today References: <08A0A863637F1349BBFD83A96B27A50A120138@uwhis-xchng3.uwhis.hosp.wisc.edu> <346E5878979BA54FB4B0BFD6AD93B9B9B01EE96359@EXCHMBC1.ad.ah.local> Message-ID: <005301c8c11b$129cc1c0$0302a8c0@yourxhtr8hvc4p> me either JTT ----- Original Message ----- From: "Mahoney,Janice A" To: "Ingles Claire" ; Sent: Wednesday, May 28, 2008 10:28 AM Subject: [Histonet] RE: NSH teleconference today Me either!!!!! Janice Mahoney HT(ASCP) Histology/Cytology Coordinator Alegent Health Laboratory 4955 F Street Omaha, NE (402)717-2889 fax(402)717-5231 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire [CIngles@uwhealth.org] Sent: Wednesday, May 28, 2008 9:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NSH teleconference today Help! I don't seem to have recieved the e-mail giving me the site to download the lecture materials. Anyone have it? Claire _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. 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Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Wed May 28 18:58:52 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed May 28 18:58:58 2008 Subject: [Histonet] Formalin alternative Message-ID: <582736990805281658k1d7f7b49n5f35043452a1be1@mail.gmail.com> Hi, If you look into the archives, you'll see I have ranted against using alternative fixatives in the past. So it may be a bit of a shock to see me recommending one. I still maintain the need for formalin as a routine clinical fixative, but for research the same rules don't necessarily apply. Obviously it is important to understand that the results do not necessarily compare to that of FFPE tissues. That said, Streck fixative works BEAUTIFULLY especially on mouse lungs. The tissues are well preserved, stain nicely in H&E and have performed well in most IHC that I have seen. Unfortunately I am not sure where to get this anymore. I think the company was either bought out or just closed down. If anyone knows if some company bought out this patent I would really like to know about it. I guess it isn't very helpful recommending a fixative that may not even be on the market anymore, but it works well ... for what it's worth. Good luck, Amos Brooks Message: 23 Date: Wed, 28 May 2008 11:12:43 -0400 From: stephanie.d.rivera@gsk.com Subject: [Histonet] Formalin alternative fixative for rodent and large animal To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Hello histoworld, Does anyone have any suggestions for altenative fixative to formalin? I still need to maintain quality of IHC and H&E stain and morphology. I've come across Prefer and Notoxhisto. Are there any others for animal tissue that anyone have tried? From amosbrooks <@t> gmail.com Wed May 28 19:08:21 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed May 28 19:08:34 2008 Subject: [Histonet] Formalin alternative Message-ID: <582736990805281708s59a60cb2u5824f95399d1ae82@mail.gmail.com> Interesting, After my saying it worked well :-) What tissues were you working with that the morpho was poor? (So I don't try it & get the same results) We don't use it for everything, so I guess it could be tissue dependant. I swear it is the shiznit for these mouse lungs. Different strokes I guess, Amos Message: 1 Date: Wed, 28 May 2008 10:32:33 -0500 From: Jackie M O'Connor > Subject: Re: [Histonet] Formalin alternative fixative for rodent and large animal To: stephanie.d.rivera@gsk.com Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" In my opinion, for pharma research, there is no worthwhile formalin substitute that will give consistent IHC results. I have tried STF (which was discontinued) and other subs - finally went back to formalin. With STF, the morphology of routine H+E's was horrible. I'm sure there are differing opinions out there - after all, this is histonet. Jackie O' From amosbrooks <@t> gmail.com Wed May 28 19:16:44 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed May 28 19:16:47 2008 Subject: [Histonet] My last hope for FFPE anti rat CD4 Message-ID: <582736990805281716t449f3883o9bcedc773b3583be@mail.gmail.com> Jackie, I just happened to have a Biocare Medical catalog within arms reach (scary since I'm home ... I've really gotta get a life!) and looked up their CD4 as an off chance and guess what? Under Species Reactivity they say Human, Mouse & Rat! I can't avouch for this antibody as I haven't used it on rat, but it's in the book. Try their tech support to make sure. It is cat# CM153AA clone BC/1F6. Hope this helps, Amos Message: 5 Date: Wed, 28 May 2008 11:28:29 -0500 From: Jackie M O'Connor > Subject: [Histonet] My last hope for FFPE anti rat CD4 To: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" This topic has been beaten to death - but I'll ask again just incase there has been a miracle breakthrough. Anyone know of an anti-rat CD4 antibody that will work in FFPE? Don't yell at me, please. Thanks, Jackie O' From gayle.callis <@t> bresnan.net Wed May 28 20:03:28 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Wed May 28 20:03:41 2008 Subject: [Histonet] My last hope for FFPE anti rat CD4 References: <582736990805281716t449f3883o9bcedc773b3583be@mail.gmail.com> Message-ID: <001e01c8c127$d0803e90$6401a8c0@DHXTS541> Jackie, Are you looking for a rat antiMouse CD4 or the reverse, mouse antiRat CD4? Gayle Callis ----- Original Message ----- From: "Amos Brooks" To: ; Sent: Wednesday, May 28, 2008 6:16 PM Subject: [Histonet] My last hope for FFPE anti rat CD4 > Jackie, > I just happened to have a Biocare Medical catalog within arms reach > (scary since I'm home ... I've really gotta get a life!) and looked up > their > CD4 as an off chance and guess what? Under Species Reactivity they say > Human, Mouse & Rat! I can't avouch for this antibody as I haven't used it > on > rat, but it's in the book. Try their tech support to make sure. It is cat# > CM153AA clone BC/1F6. > > Hope this helps, > Amos > > > Message: 5 > Date: Wed, 28 May 2008 11:28:29 -0500 > From: Jackie M O'Connor > >> > Subject: [Histonet] My last hope for FFPE anti rat CD4 > To: histonet@lists.utsouthwestern.edu, > histonet-bounces@lists.utsouthwestern.edu > Message-ID: > > > > Content-Type: text/plain; charset="US-ASCII" > > This topic has been beaten to death - but I'll ask again just incase there > has been a miracle breakthrough. > > Anyone know of an anti-rat CD4 antibody that will work in FFPE? > > Don't yell at me, please. > > Thanks, > > Jackie O' > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Wed May 28 22:43:17 2008 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed May 28 22:43:25 2008 Subject: [Histonet] Rate of formalin penetration in human brain sections Message-ID: Dear Karen,
 
"One of your investigators" n money questions are in:
 < Histology. Oxford: Clarendon Press.
 
Baker, J.R. (1958) Principles of Methuen.
  
These are classics in the field of fixation an textb
 
& UWO
London, Canada
=
 ----
Original Message -----
F rom: Karen_Skish@rush.edu
Date: Tuesday, May 27, 20 penetration histonet@lists.utsouthwestern. Hi--
> One of our investigator approximate rate
> of fixation < human brain tissue, independent of any formaldehyde d
> effects.
> In other words, very small or very thin piece of human
> brain tissu e,
> what is the fixation rate? He found published da like to
> should brain tissue. He is looki
> temperature, bu appreciated.
> Thanks Skish, MS, PA(ASCP)MT
> Path & Manager, Neuropathology Lab
& Alzheimer's Disease Center
> Cohn Research Bu ilding, Lab 441
> 1735 West Harrison Street
> ____________ _______________________ 5F list< Histonet@lists.utsouthwestern.edu
> ht tp://lists.utsouthwestern.edu/mailman/listinfo/histonet
3C From timscase <@t> gmail.com Thu May 29 03:53:09 2008 From: timscase <@t> gmail.com (Tim Scase) Date: Thu May 29 03:53:24 2008 Subject: [Histonet] Senior Histology technician required for new laboratory in, Bristol, UK Message-ID: <483E6EF5.5060308@gmail.com> Dear Histonetters, I'd like to bring to your attention a senior position that we have available for a highly capable and enthusiastic histology technician for a new veterinary histopathology company that we are setting up in Bristol, UK. The details of the position are pasted in below. If you have any friends in the veterinary or human histology sector in the UK that you think might be interested, please pass the message on! Best wishes, Tim Tim Scase BSc BVM&S MRCVS Phd Dip.ACVP Director of Pathology Bridge Pathology Ltd. Job Title: Chief Laboratory Technician Bridge Pathology Ltd. is a recently registered company located in Bristol, that will specialise in the processing of biopsy specimens submitted by veterinary surgeons. Tissue samples will be examined histologically and reports generated by veterinary pathologists, with an emphasis on providing the veterinary surgeon with as much specific, detailed and clinically useful information as possible. Where applicable, histochemical and immunohistochemical methods will be used to characterise the tissues further. In addition, samples received through the laboratory will be used for clinical research studies to further our understanding of animal disease and to develop prognostic and diagnostic assays to benefit veterinary patients. Results from these research studies will be submitted for publication in the scientific literature. We hope to establish Bridge Pathology Ltd. as the UK's premier diagnostic and research-focussed (and friendliest) veterinary histopathological laboratory. You will work with the Director of Pathology to set up, manage and develop the histopathology and immunohistochemistry laboratory facillities and oversee the day-to-day operation of the histopathology and immunohistochemistry laboratories. This will include routine preparation of histological and immunohistochemical-stained sections of submitted biopsy tissues for diagnostic and research purposes. A successful applicant will have: 1) A minimum of a bachelor of science degree. 2) At least 3 years experience managing and running a histopathology or immunohistochemistry laboratory. 3) At least 3 years experience of preparation of tissue specimens for routine histopathological examination. 4) At least 2 years experience of preparing histochemical and immunohistochemical stained sections. 5) Meticulous working habits with meticulous documentation of samples and processing thereof. 6) Experience developing laboratory standard operating procedures. 7) The ability to work well in a close-knit, friendly team. 8) The ability to work efficiently and to tight deadlines. 9) Good communication skills. 10) Basic computer skills. From lpwenk <@t> sbcglobal.net Thu May 29 04:15:35 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu May 29 04:15:41 2008 Subject: [Histonet] ?Quicker Copper Stain In-Reply-To: Message-ID: <001301c8c16c$8dbe5930$cce22d4b@HPPav2> Below is our procedure. In a 60 degree C oven/incubater/waterbath, it takes a little over 1 hour. With a microwave, it will be done in about 10 mintues. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 - - - - - - - - - COPPER - RHODANINE PREPARED BY: Peggy A. Wenk, BS, HTL(ASCP)SLS PURPOSE: This stain demonstrates increased amounts of copper, but may not be specific for copper. Copper is normally found in small amounts in the body. Abnormal increased deposits of copper can be found in Wilson disease, and in cirrhotic liver. In Wilson disease, copper accumulates in certain organs, such as the liver and brain, causing scarring and cirrhosis. In cirrhotic livers, the scarring may trap the copper, rather than the copper causing the scarring. PRINCIPLE: The rhodanine may be specific for the protein to which the copper is bound. It may be demonstrating other metals. This stain is more sensitive than the rubeanic acid method, but may not be as specific. Hematoxylin is used as a counterstain. FIXATION: Any well fixed tissue. 10% neutral buffered formalin preferred. TECHNIQUE: Cut paraffin sections at 10 um. CONTROL: Section of tissue with copper QUALITY CONTROL: 1. Sections should be 6-10 um thick, to locate minimal deposits. EQUIPMENT: Balance, Erlenmeyer flasks, graduated cylinders, magnetic stirrer, forceps. CAUTION: Follow standard safety procedures when preparing stains. RHODANINE (5(p-dimethylaminobenzylidine) should be kept away from sources of heat. Can violently decompose at elevated temperatures. May be harmful if swallowed. HEMATOXYLIN is incompatible with oxidizers & alkalies. Store separate from them. AMMONIUM HYDROXIDE may cause severe skin and eye burns. Vapors are irritating to eyes and respiratory tract. Harmful if swallowed or inhaled. REAGENTS: STOCK RHODANINE SOLUTION Rhodanine (5(p-dimethylaminobenzylidine)) (C12H12N2OS2) 0.2 g Absolute ethanol, reagent 100.0 mL Stir together several hours to make saturated solution. Store at room temperature. Stable 4-6 months. WORKING RHODANINE SOLUTION Stock rhodanine solution 3.0 mL Distilled water 40.0 mL JUST BEFORE USE, mix together. Good for one day only. ALUM HEMATOXYLIN Use Mayer or Gill hematoxylin used in H&E set up. DILUTE AMMONIA WATER Use dilute ammonia water used in H&E set-up. PROCEDURE - Rhodanine: 1. Deparaffinize and hydrate sections through graded alcohol to distilled water. 2. Place slides in WORKING rhodanine solution in 60o C. oven 1 hour (OR, heat in 750 watt microwave oven on HIGH for 30 seconds. DO NOT LEAVE UNATTENDED. Allow to set on counter at room temperature for 5 minutes.) 3. Rinse in distilled water, 3-5 changes 5-10 seconds each 4. Stain lightly in alum hematoxylin 10 seconds 5. Wash in running water 10 seconds 6. Blue in dilute ammonia water 2-3 seconds 7. Wash in running water 5 minutes 8. Dehydrate through graded alcohols and clear in xylene. 9. Coverslip using a synthetic mounting media. RESULTS: Copper deposits orange/red Heavy metals (mercury, silver, cadmium) orange/red Bile yellow Red blood cells yellow Lipofuchsin golden Nuclei blue PROCEDURAL NOTES: 1. Avoid fixatives with heavy metals (mercury, zinc), as possible false-negative staining have been reported. 2. This is not a specific test for copper. This demonstrates the protein to which heavy metals, such as copper, would bind. Therefore, it may be demonstrating other metals, such as silver or cadmium. 3. Time given for the microwave procedure is for a 750 watt microwave oven. Adjust time accordingly for microwave ovens with other wattage. Solution should be heated to about 80o C. 4. As the Working Rhodanine solution is alcoholic, it heats up faster than an aqueous solution heated in a microwave oven. As a result, the working rhodanine solution boils very quickly when heated in the microwave oven. Do not leave unattended. 5. Fading of staining after coverslipping is common. 6. Stain may also be done at 37o C. Stain for 18 hours. REFERENCES: Bancroft JD, Stevens A: Theory and Practice of Histological Techniques, 3rd ed. New York, NY, Churchill Livingstone, 1990. Carson FL: Histotechnology: A Self-Instructional Text, Chicago, IL, ASCP Press, 1990. Sheehan DC, Hrapchak BB: Theory and Practice of Histotechnology, 2nd edition. Columbus, Ohio, Battelle Press, 1980. Vacca L: laboratory Manual of Histochemistry. New York, NY, Raven Press, 1985. * Source of procedure is unknown. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JMaslanka@stpetes.org Sent: Wednesday, May 28, 2008 1:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ?Quicker Copper Stain Hi, does anyone have a quicker procedure for staining copper in liver other than the overnight method? If so would you please share. Thanks Joe Maslanka Cyto/Histo Coord. St Peter's Laboratory " Not everything that can be counted counts..... Not everything that counts can be counted." Albert Einstein _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Linda.Watson <@t> bms.com Thu May 29 05:20:47 2008 From: Linda.Watson <@t> bms.com (Linda M Watson) Date: Thu May 29 05:20:51 2008 Subject: [Histonet] RE: Biotin free detection of goat primary antibodies In-Reply-To: References: Message-ID: <483E837F.4000008@bms.com> Hello Everyone, BioCare Medical makes a great reagent that detects goat primary antibodies and is a polymer system. This biotin free system eliminates the biotin background problems that is most often enhanced in combination with HIER and ABC methods of detection. Linda C.M. van der Loos wrote: >Dear Min-Han,We solved this problem with a 3-step approach: goat primary - rabbit anti-goat IgG - anti-rabbit polymer/HRP. The second step we purchased from Southern Biotech Associates, Birmingham, AL. Dilution of the rabbit anti-goat IgG (human IgG adsorbed) came out at 1:2000-5000 (30 min, RT). Any anti-rabbit polymer/HRP will do. Gayle is fully right with respect to the negative control: find a non-immune goat IgG with a known IgG protein concentration. You need that to calculate the final dilution.Hope this helps a bit.Cheers, ChrisChris van der Loos, PhD >Dept. of Pathology >Academic Medical Center M2-230 >Meibergdreef 9 >NL-1105 AZ Amsterdam >The NetherlandsDate: Tue, 27 May 2008 10:55:59 +0800 >From: "Min-Han Tan" >Subject: [Histonet] Biotin free detection of goat primary antibodies >To: histonet@lists.utsouthwestern.edu > >Dear all, > >I would like to enquire - I am using a goat primary antibody currently, and >my negative control (omitting primary antibody) is showing background >staining - this is likely a result of biotin. > >I'd like to enquire if any one is familiar with biotin free systems that can >detect goat antibodies. A search of the Dako / Envision product line does >not yield any results. I am very reluctant to add an avidin-biotin blocking >step to what is already an overnight incubation. > >Thanks! > > > From barbwebb <@t> webtv.net Thu May 29 06:38:11 2008 From: barbwebb <@t> webtv.net (Barbara Webb) Date: Thu May 29 06:38:20 2008 Subject: [Histonet] off-topic Message-ID: Fascinating article - A contagious cancer of Tasmanian Devils- http://online.wsj.com/article_email/SB121192224081523893-lMyQjAxMDI4MTIxOTkyMjkyWj.html Barbara Webb HTL (ASCP) From Jackie.O'Connor <@t> abbott.com Thu May 29 07:39:41 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu May 29 07:40:04 2008 Subject: [Histonet] Re: My last hope for FFPE anti rat CD4 In-Reply-To: <582736990805281716t449f3883o9bcedc773b3583be@mail.gmail.com> Message-ID: Thanks, Amos - I called Biocare and they no longer claim this antibody works on rat. Their current product data sheet reflects only human reactivity. boo. "Amos Brooks" 05/28/2008 07:16 PM To Jackie.O'Connor@abbott.com, "histonet@lists.utsouthwestern.edu" cc Subject My last hope for FFPE anti rat CD4 Jackie, I just happened to have a Biocare Medical catalog within arms reach (scary since I'm home ... I've really gotta get a life!) and looked up their CD4 as an off chance and guess what? Under Species Reactivity they say Human, Mouse & Rat! I can't avouch for this antibody as I haven't used it on rat, but it's in the book. Try their tech support to make sure. It is cat# CM153AA clone BC/1F6. Hope this helps, Amos Message: 5 Date: Wed, 28 May 2008 11:28:29 -0500 From: Jackie M O'Connor Subject: [Histonet] My last hope for FFPE anti rat CD4 To: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: < OFA64947FF.E0CCE76E-ON86257457.005A4D22-86257457.005A8477@abbott.com> Content-Type: text/plain; charset="US-ASCII" This topic has been beaten to death - but I'll ask again just incase there has been a miracle breakthrough. Anyone know of an anti-rat CD4 antibody that will work in FFPE? Don't yell at me, please. Thanks, Jackie O' From tp2 <@t> medicine.wisc.edu Thu May 29 08:45:19 2008 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Thu May 29 09:14:49 2008 Subject: [Histonet] RE: Biotin free detection of goat primary antibodies Message-ID: <483E6D1F020000DF0000A9A7@gwmail.medicine.wisc.edu> I tried the BioCare Medical goat HRP polymer for the first time last week and was very pleased with the results. Tom Pier >>> Linda M Watson 05/29/08 5:24 AM >>> Hello Everyone, BioCare Medical makes a great reagent that detects goat primary antibodies and is a polymer system. This biotin free system eliminates the biotin background problems that is most often enhanced in combination with HIER and ABC methods of detection. Linda C.M. van der Loos wrote: >Dear Min-Han,We solved this problem with a 3-step approach: goat primary - rabbit anti-goat IgG - anti-rabbit polymer/HRP. The second step we purchased from Southern Biotech Associates, Birmingham, AL. Dilution of the rabbit anti-goat IgG (human IgG adsorbed) came out at 1:2000-5000 (30 min, RT). Any anti-rabbit polymer/HRP will do. Gayle is fully right with respect to the negative control: find a non-immune goat IgG with a known IgG protein concentration. You need that to calculate the final dilution.Hope this helps a bit.Cheers, ChrisChris van der Loos, PhD >Dept. of Pathology >Academic Medical Center M2-230 >Meibergdreef 9 >NL-1105 AZ Amsterdam >The NetherlandsDate: Tue, 27 May 2008 10:55:59 +0800 >From: "Min-Han Tan" >Subject: [Histonet] Biotin free detection of goat primary antibodies >To: histonet@lists.utsouthwestern.edu > >Dear all, > >I would like to enquire - I am using a goat primary antibody currently, and >my negative control (omitting primary antibody) is showing background >staining - this is likely a result of biotin. > >I'd like to enquire if any one is familiar with biotin free systems that can >detect goat antibodies. A search of the Dako / Envision product line does >not yield any results. I am very reluctant to add an avidin-biotin blocking >step to what is already an overnight incubation. > >Thanks! > > > From ian.montgomery <@t> bio.gla.ac.uk Thu May 29 09:15:19 2008 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Thu May 29 09:15:30 2008 Subject: [Histonet] Sarcoplasmic reticulum. Message-ID: <1C225E17F05A4FD8A92E5F3A24F98C72@IBLS.GLA.AC.UK> Just been asked, "is it possible to stain the sarcoplasmic reticulum at the light level." EM it jumps out at you but I'm a bit stumped for LM. CaATPase, mmmm, any thoughts? Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. From Karen_Skish <@t> rush.edu Thu May 29 10:07:25 2008 From: Karen_Skish <@t> rush.edu (Karen_Skish@rush.edu) Date: Thu May 29 10:10:18 2008 Subject: [Histonet] Rate of formalin penetration in human brain sections In-Reply-To: Message-ID: Thank you to all for the information provided. My investigator had already located this information and was happy to see that he is on the right track with his project. On a side note, I often find this group very informative and helpful, but at other times I am baffled by the responses. If you believe that a inquiry is due to an individual's laziness, wouldn't it be best to either ask for additional information so that you may provide "food for thought"; or ignore the question outright? John Kiernan 05/28/2008 10:43 PM To Karen_Skish@rsh.net cc histonet@lists.utsouthwestern.edu Subject Re: [Histonet] Rate of formalin penetration in human brain sections Dear Karen, "One of your investigators" needs to visit his library. Perhaps even spend $30 of his grant money on on a book or two for the lab. His questions are not new. They are addressed well in: Mann, G. (1902) Physiological Histology. Oxford: Clarendon Press. Baker, J.R. (1958) Principles of Biological Microtechnique. London: Methuen. Horobin, R.W. (1982) Histochemistry. Stuttgart: Fischer. These are classics in the field of fixation and staining. There are also very recent textbooks in the field. One of them is by me. John Kiernan Anatomy, UWO London, Canada = = = ---- Original Message ----- From: Karen_Skish@rush.edu Date: Tuesday, May 27, 2008 17:18 Subject: [Histonet] Rate of formalin penetration in human brain sections To: histonet@lists.utsouthwestern.edu > Hi-- > One of our investigators is interested in the approximate rate > of fixation > of human brain tissue, independent of any formaldehyde diffusion > effects. > In other words, in a very small or very thin piece of human > brain tissue, > what is the fixation rate? He found published data for rat > kidney, but > would like to try to at least determine if the fixation rate > should be > higher or lower in human brain tissue. He is looking for data > for room > temperature, but any information would be greatly appreciated. > Thanks! > Karen M Skish, MS, PA(ASCP)MT > Pathologists' Assistant & Manager, Neuropathology Lab > Rush Alzheimer's Disease Center > Cohn Research Building, Lab 441 > 1735 West Harrison Street > Chicago IL 60612 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu May 29 10:36:46 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 29 10:36:51 2008 Subject: [Histonet] Rate of formalin penetration in human brain sections In-Reply-To: Message-ID: <87205.66032.qm@web65702.mail.ac4.yahoo.com> This is one of the less thankful "thank yous" I have read in HistoNet. Ren? J. Karen_Skish@rush.edu wrote: Thank you to all for the information provided. My investigator had already located this information and was happy to see that he is on the right track with his project. On a side note, I often find this group very informative and helpful, but at other times I am baffled by the responses. If you believe that a inquiry is due to an individual's laziness, wouldn't it be best to either ask for additional information so that you may provide "food for thought"; or ignore the question outright? John Kiernan 05/28/2008 10:43 PM To Karen_Skish@rsh.net cc histonet@lists.utsouthwestern.edu Subject Re: [Histonet] Rate of formalin penetration in human brain sections Dear Karen, "One of your investigators" needs to visit his library. Perhaps even spend $30 of his grant money on on a book or two for the lab. His questions are not new. They are addressed well in: Mann, G. (1902) Physiological Histology. Oxford: Clarendon Press. Baker, J.R. (1958) Principles of Biological Microtechnique. London: Methuen. Horobin, R.W. (1982) Histochemistry. Stuttgart: Fischer. These are classics in the field of fixation and staining. There are also very recent textbooks in the field. One of them is by me. John Kiernan Anatomy, UWO London, Canada = = = ---- Original Message ----- From: Karen_Skish@rush.edu Date: Tuesday, May 27, 2008 17:18 Subject: [Histonet] Rate of formalin penetration in human brain sections To: histonet@lists.utsouthwestern.edu > Hi-- > One of our investigators is interested in the approximate rate > of fixation > of human brain tissue, independent of any formaldehyde diffusion > effects. > In other words, in a very small or very thin piece of human > brain tissue, > what is the fixation rate? He found published data for rat > kidney, but > would like to try to at least determine if the fixation rate > should be > higher or lower in human brain tissue. He is looking for data > for room > temperature, but any information would be greatly appreciated. > Thanks! > Karen M Skish, MS, PA(ASCP)MT > Pathologists' Assistant & Manager, Neuropathology Lab > Rush Alzheimer's Disease Center > Cohn Research Building, Lab 441 > 1735 West Harrison Street > Chicago IL 60612 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Thu May 29 11:57:43 2008 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu May 29 11:57:25 2008 Subject: [Histonet] Sarcoplasmic reticulum. In-Reply-To: <1C225E17F05A4FD8A92E5F3A24F98C72@IBLS.GLA.AC.UK> References: <1C225E17F05A4FD8A92E5F3A24F98C72@IBLS.GLA.AC.UK> Message-ID: <483EE087.7080502@umdnj.edu> Hi Ian: Many years ago (1980's) Lee Peachy stained SR (with silver?) to study its distribution by high voltage TEM. I would think that the methods he used could be of use for your project. Look his work up in PubMed, or try Google. Geoff Ian Montgomery wrote: > Just been asked, "is it possible to stain the sarcoplasmic > reticulum at the light level." EM it jumps out at you but I'm a bit stumped > for LM. CaATPase, mmmm, any thoughts? > > Ian. > > > > Dr. Ian Montgomery, > > Histotechnology, > > I.B.L.S. Support Unit, > > Thomson Building, > > University of Glasgow, > > Glasgow, > > G12 8QQ. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From judi.ford <@t> roche.com Thu May 29 12:02:22 2008 From: judi.ford <@t> roche.com (Ford, Judi) Date: Thu May 29 12:02:31 2008 Subject: [Histonet] Shaffer's Fixative Message-ID: Hi everyone, Our lab has received blocks in which the rat livers were fixed in Shaffer's fixative. I don't know much about this fixative, other than it is alcohol based, but it is difficult to hydrate the tissue and once cut the tissue on the slide falls off, even when using '+' slides. The tech who is cutting the liver tissue noticed that even before staining the slides, as the tissue is drying, it will crack and curl up. She has cut and recut the blocks; sometimes with improvement but often without. The first batch was soaked on an ice tray for 30 min. and the second time she soaked them for an hour. We also don't know how the tissue was processed (timing, etc). The slides are being stained with PAS. If anyone has idea on why tissue may be falling off and how to keep it from happening we'd love to hear suggestions. Cheers, Judi Ford Roche Palo Alto Palo Alto, CA From Shirley_PHUA <@t> hsa.gov.sg Thu May 29 13:04:19 2008 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Thu May 29 13:07:00 2008 Subject: [Histonet] Shirley Phua is out-of-office ... Message-ID: I will be out of the office from 30-05-2008 to 30-05-2008. I'll be away on course on 30 May 2008. Pathologists: I will process your requests when I return. If urgent, please forward your email to Henry_Kyaw@hsa.gov.sg From jkiernan <@t> uwo.ca Thu May 29 13:50:57 2008 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu May 29 13:51:02 2008 Subject: [Histonet] Shaffer's Fixative Message-ID: There are two Schaffer's fixatives (1908, 1918), almost identical: about 30% formalin in about 50% alcohol. There is also a Schaffner's (1918) fixative, which is 0.3% chromium trioxide and 0.7% acetic acid in water. A mixture of that kind would preserve chromosomes and mitotic spindles well, while largely destroying cytoplasm. Could the problem with your tissue be faulty processing rather than the fixation? Try melting down a few blocks, going back to 70% alcohol and then reprocessing. ----- Original Message ----- From: "Ford, Judi" Date: Thursday, May 29, 2008 13:03 Subject: [Histonet] Shaffer's Fixative To: histonet@lists.utsouthwestern.edu > Hi everyone, > > > > Our lab has received blocks in which the rat livers were fixed in > Shaffer's fixative. I don't know much about this fixative, other > than it > is alcohol based, but it is difficult to hydrate the tissue and > once cut > the tissue on the slide falls off, even when using '+' slides. > The tech > who is cutting the liver tissue noticed that even before > staining the > slides, as the tissue is drying, it will crack and curl up. She > has cut > and recut the blocks; sometimes with improvement but often > without. The > first batch was soaked on an ice tray for 30 min. and the second time > she soaked them for an hour. We also don't know how the tissue was > processed (timing, etc). The slides are being stained with PAS. > > > > If anyone has idea on why tissue may be falling off and how to > keep it > from happening we'd love to hear suggestions. > > > > Cheers, > > > > Judi Ford > > Roche Palo Alto > > Palo Alto, CA > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JHAPPEL <@t> PARTNERS.ORG Thu May 29 14:11:21 2008 From: JHAPPEL <@t> PARTNERS.ORG (Happel, James F.) Date: Thu May 29 14:11:32 2008 Subject: [Histonet] Immuno Controls Message-ID: Good Day Histonetters! For immuno cases, how are you handling the control tissue question? Are you putting a piece of control tissue on each slide or are you using a control slide for each ab performed on a given day. We are using Ventana'e Benchmark for the lion's share of our immunos. Thanks! James Happel Massachusetts General Hospital The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From JCBRITTON <@t> Cheshire-Med.COM Thu May 29 14:28:41 2008 From: JCBRITTON <@t> Cheshire-Med.COM (Josie Britton) Date: Thu May 29 14:28:50 2008 Subject: [Histonet] Immuno Controls In-Reply-To: References: Message-ID: We are using 1 control slide with each of our batches per antibody and 1 negative control slide per patient case. Hope this helps! Josie Britton HT Cheshire Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Happel, James F. Sent: Thursday, May 29, 2008 3:11 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Immuno Controls Good Day Histonetters! For immuno cases, how are you handling the control tissue question? Are you putting a piece of control tissue on each slide or are you using a control slide for each ab performed on a given day. We are using Ventana'e Benchmark for the lion's share of our immunos. Thanks! James Happel Massachusetts General Hospital The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. From rjbuesa <@t> yahoo.com Thu May 29 14:29:51 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 29 14:29:55 2008 Subject: [Histonet] Immuno Controls In-Reply-To: Message-ID: <85217.80453.qm@web65709.mail.ac4.yahoo.com> I used the DAKO autostainer and the cases' sections were placed in slides already containing a tissue control section in a way that both were "stained" simultaneously. I only used one single control for a whole series of cases when doing Her2neu, or for research studies for our residents. Ren? J. "Happel, James F." wrote: From mburton1 <@t> bu.edu Thu May 29 15:15:54 2008 From: mburton1 <@t> bu.edu (Mark A Burton) Date: Thu May 29 15:16:12 2008 Subject: [Histonet] Immuno Controls In-Reply-To: <85217.80453.qm@web65709.mail.ac4.yahoo.com> References: <85217.80453.qm@web65709.mail.ac4.yahoo.com> Message-ID: <001901c8c1c8$cc6fad30$654f0790$@edu> I'm sure this discussion must be in the archives but my understanding of how the Ventana Benchmark works is that there is a plunger that presses on a dispenser bottle to deliver the antibodies and reagents to the slide. If the dispenser bottle is not primed or if there is some type of interference than you would not get any antibody or reagent delivered to your slide. Without a control on each slide, there is no way to know. Is this a legitimate argument? or is the amount of time and tissue needed to accomplish this too costly? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, May 29, 2008 3:30 PM To: Happel, James F.; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Immuno Controls I used the DAKO autostainer and the cases' sections were placed in slides already containing a tissue control section in a way that both were "stained" simultaneously. I only used one single control for a whole series of cases when doing Her2neu, or for research studies for our residents. Ren? J. "Happel, James F." wrote: _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree <@t> uwhealth.org Thu May 29 15:29:53 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Thu May 29 15:29:58 2008 Subject: [Histonet] Immuno Controls In-Reply-To: Message-ID: We expect our pathologists to share positive tissue controls for cases that are all run in the same batch. The only exception would be if the slides were going to be read at a remote location making sharing problematic. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Happel, James F. Sent: Thursday, May 29, 2008 2:11 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Immuno Controls Good Day Histonetters! For immuno cases, how are you handling the control tissue question? Are you putting a piece of control tissue on each slide or are you using a control slide for each ab performed on a given day. We are using Ventana'e Benchmark for the lion's share of our immunos. Thanks! James Happel Massachusetts General Hospital The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu May 29 15:38:05 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 29 15:38:10 2008 Subject: [Histonet] Immuno Controls In-Reply-To: <001901c8c1c8$cc6fad30$654f0790$@edu> Message-ID: <500034.26153.qm@web65707.mail.ac4.yahoo.com> Ah the joys of using Ventana are endless! Ren? J. Mark A Burton wrote: I'm sure this discussion must be in the archives but my understanding of how the Ventana Benchmark works is that there is a plunger that presses on a dispenser bottle to deliver the antibodies and reagents to the slide. If the dispenser bottle is not primed or if there is some type of interference than you would not get any antibody or reagent delivered to your slide. Without a control on each slide, there is no way to know. Is this a legitimate argument? or is the amount of time and tissue needed to accomplish this too costly? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, May 29, 2008 3:30 PM To: Happel, James F.; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Immuno Controls I used the DAKO autostainer and the cases' sections were placed in slides already containing a tissue control section in a way that both were "stained" simultaneously. I only used one single control for a whole series of cases when doing Her2neu, or for research studies for our residents. Ren? J. "Happel, James F." wrote: _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dspears <@t> mmci.org Thu May 29 16:29:32 2008 From: dspears <@t> mmci.org (Dana Spears) Date: Thu May 29 16:29:55 2008 Subject: [Histonet] Immuno Controls Message-ID: Histonetters, In my opinion, it is always better for patient safety to have the control and the patient tissue on the same slide - no matter what platform you are running (or even if you were running them manually!). I've used BioGenex, DAKO, Ventana, and manual methods and I feel it is better for the patient AND cheaper if the control is on the same slide. No matter how you are running your IHCs, there is the slight possibility that the instrument could falter or you could forget to apply a reagent to a particular slide - better to have the control on there and you KNOW it worked. Sure, there is the argument that you go through more control blocks, but if you align your chuck every time and are careful, that additional tissue is a small price to pay for better patient care!!! Dana Spears, HTL(ASCP) Anatomic Pathology Manager Methodist Medical Center (309) 672-4930 (office) (309) 255-7214 (cell) dspears@mmci.org >>> "Mark A Burton" 5/29/2008 3:15 PM >>> I'm sure this discussion must be in the archives but my understanding of how the Ventana Benchmark works is that there is a plunger that presses on a dispenser bottle to deliver the antibodies and reagents to the slide. If the dispenser bottle is not primed or if there is some type of interference than you would not get any antibody or reagent delivered to your slide. Without a control on each slide, there is no way to know. Is this a legitimate argument? or is the amount of time and tissue needed to accomplish this too costly? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, May 29, 2008 3:30 PM To: Happel, James F.; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Immuno Controls I used the DAKO autostainer and the cases' sections were placed in slides already containing a tissue control section in a way that both were "stained" simultaneously. I only used one single control for a whole series of cases when doing Her2neu, or for research studies for our residents. Ren? J. "Happel, James F." wrote: _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE: This message is a PRIVATE communication. This e-mail may contain confidential or proprietary information that may be considered legally privileged. It is intended only for the named recipient(s). If an addressing or transmission error has misdirected the e-mail, please notify the author by replying to this message. If you are not the named recipient, you are not authorized to use, disclose, distribute, copy, print, or rely on this e-mail, and should immediately delete it from your computer system. Thank you for your assistance with this matter. From Jeannette.Mitchell <@t> vtmednet.org Thu May 29 16:51:45 2008 From: Jeannette.Mitchell <@t> vtmednet.org (Mitchell, Jeannette M.) Date: Thu May 29 16:53:12 2008 Subject: [Histonet] Immuno Controls In-Reply-To: References: Message-ID: I second that. Autostainers are great but what if it does faulter.... you have just put out a slide that is negative... but is it really!!! Jeannette Mitchell R&D Histotechnologist FAHC Burlington,VT ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dana Spears [dspears@mmci.org] Sent: Thursday, May 29, 2008 5:29 PM To: Mark A Burton; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Immuno Controls Histonetters, In my opinion, it is always better for patient safety to have the control and the patient tissue on the same slide - no matter what platform you are running (or even if you were running them manually!). I've used BioGenex, DAKO, Ventana, and manual methods and I feel it is better for the patient AND cheaper if the control is on the same slide. No matter how you are running your IHCs, there is the slight possibility that the instrument could falter or you could forget to apply a reagent to a particular slide - better to have the control on there and you KNOW it worked. Sure, there is the argument that you go through more control blocks, but if you align your chuck every time and are careful, that additional tissue is a small price to pay for better patient care!!! Dana Spears, HTL(ASCP) Anatomic Pathology Manager Methodist Medical Center (309) 672-4930 (office) (309) 255-7214 (cell) dspears@mmci.org >>> "Mark A Burton" 5/29/2008 3:15 PM >>> I'm sure this discussion must be in the archives but my understanding of how the Ventana Benchmark works is that there is a plunger that presses on a dispenser bottle to deliver the antibodies and reagents to the slide. If the dispenser bottle is not primed or if there is some type of interference than you would not get any antibody or reagent delivered to your slide. Without a control on each slide, there is no way to know. Is this a legitimate argument? or is the amount of time and tissue needed to accomplish this too costly? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, May 29, 2008 3:30 PM To: Happel, James F.; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Immuno Controls I used the DAKO autostainer and the cases' sections were placed in slides already containing a tissue control section in a way that both were "stained" simultaneously. I only used one single control for a whole series of cases when doing Her2neu, or for research studies for our residents. Ren? J. "Happel, James F." wrote: _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE: This message is a PRIVATE communication. This e-mail may contain confidential or proprietary information that may be considered legally privileged. It is intended only for the named recipient(s). If an addressing or transmission error has misdirected the e-mail, please notify the author by replying to this message. If you are not the named recipient, you are not authorized to use, disclose, distribute, copy, print, or rely on this e-mail, and should immediately delete it from your computer system. Thank you for your assistance with this matter. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcox90 <@t> yahoo.com Thu May 29 16:56:54 2008 From: jcox90 <@t> yahoo.com (Jill Cox) Date: Thu May 29 16:56:57 2008 Subject: [Histonet] Pregnancy Testing in Pathology lab Message-ID: <317108.99368.qm@web56805.mail.re3.yahoo.com> Hi Netters, I was just asked by one of our Doctors if we can read pregnancy tests in the lab. They want to start offering this to clients but don't know the rules on this. Is anyone familiar with this? I remember way back getting one but don't remember how we handled it. Are there legalities? Do we need a license? Any help would be appreciated. Thanks!!! Jill Cox HT (ASCP) From jcox90 <@t> yahoo.com Thu May 29 16:56:59 2008 From: jcox90 <@t> yahoo.com (Jill Cox) Date: Thu May 29 16:57:03 2008 Subject: [Histonet] Pregnancy Testing in Pathology lab Message-ID: <625643.45897.qm@web56802.mail.re3.yahoo.com> Hi Netters, I was just asked by one of our Doctors if we can read pregnancy tests in the lab. They want to start offering this to clients but don't know the rules on this. Is anyone familiar with this? I remember way back getting one but don't remember how we handled it. Are there legalities? Do we need a license? Any help would be appreciated. Thanks!!! Jill Cox HT (ASCP) From yourbiomed <@t> cox.net Thu May 29 17:01:25 2008 From: yourbiomed <@t> cox.net (YourBiomed.Com ) Date: Thu May 29 17:01:32 2008 Subject: [Histonet] Anyone familiar with PSLIM Slide Printer? Message-ID: <20080529180125.UMI3F.32786.imail@fed1rmwml02> All, Anyone familiar with PSLIM Slide Printer? What do you think of this slide printer? I may be in the market for a slide printer. Any ideas, suggestions would be greatly appreciated. From lynnd01 <@t> hotmail.com Thu May 29 17:11:02 2008 From: lynnd01 <@t> hotmail.com (lynnd01@hotmail.com) Date: Thu May 29 17:11:13 2008 Subject: [Histonet] travelin tech Message-ID: Help! After 31 years, I'm thinking of working as a traveling tech. I've talked to a couple of placement agencies....sounds great. Could someone give me their experiences. It's scary to leave a job after so long for the unknown....but it also seems like a great opportunity and adventure. Thanks Lynn From lpwenk <@t> sbcglobal.net Thu May 29 19:50:14 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu May 29 19:50:21 2008 Subject: [Histonet] Sarcoplasmic reticulum. In-Reply-To: <1C225E17F05A4FD8A92E5F3A24F98C72@IBLS.GLA.AC.UK> Message-ID: <003c01c8c1ef$1f677ec0$42f02d4b@HPPav2> If the muscle is a frozen section: - Do a NADH-tetrazolium reductase which will show the mitochondria and the sarcoplasmic reticulum. Also do a SDH which which demonstrate only the mitochondria. Compare the two sections. - And throw in a modified Gomori trichrome for giggles and kicks. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian Montgomery Sent: Thursday, May 29, 2008 10:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sarcoplasmic reticulum. Just been asked, "is it possible to stain the sarcoplasmic reticulum at the light level." EM it jumps out at you but I'm a bit stumped for LM. CaATPase, mmmm, any thoughts? Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mohs76009 <@t> yahoo.com Thu May 29 20:13:30 2008 From: mohs76009 <@t> yahoo.com (Matt Bancroft) Date: Thu May 29 20:13:38 2008 Subject: [Histonet] Pregnancy Testing in Pathology lab In-Reply-To: <625643.45897.qm@web56802.mail.re3.yahoo.com> Message-ID: <71132.27051.qm@web63413.mail.re1.yahoo.com> If you are a CLIA lab, I beleive that you have to get CLIA certified to do in house pregnancy tests. You can go to CLIA.gov and do a search if you are a CLIA certified lab. Jill Cox wrote: Hi Netters, I was just asked by one of our Doctors if we can read pregnancy tests in the lab. They want to start offering this to clients but don't know the rules on this. Is anyone familiar with this? I remember way back getting one but don't remember how we handled it. Are there legalities? Do we need a license? Any help would be appreciated. Thanks!!! Jill Cox HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Fri May 30 04:10:49 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri May 30 04:11:02 2008 Subject: [Histonet] NSH bulletin (In Action) In-Reply-To: Message-ID: <000401c8c235$0e605a70$02f02d4b@HPPav2> ASCP is willing to work with states that are thinking about getting lab tech licensure. Definitely contact them for more information. http://www.ascp.org/FunctionalNavigation/certification/GetStateLicensure.asp x http://www.ascp.org/FunctionalNavigation/certification/International/StateLi censureAgencies.aspx Good overview document http://www.ascp.org/pdf/StateLicensureofLaboratoryPersonnel.aspx For information about the Mich. Licensure bill, go to Mich. Society of Clinical Laboratory Scientists web site: http://www.mscls.org/legislative_issues.htm Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: Mickie Johnson [mailto:mickie25@netzero.net] Sent: Tuesday, May 27, 2008 9:57 AM To: lpwenk@sbcglobal.net; 'Brad Miller'; 'patsy ruegg'; 'MaryAnn Dixon'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH bulletin (In Action) Hello Peggy, Thank you for your update. There has been quite a bit of discussion about this in the American College of Mohs Surgeons, American Society of Mohs Surgery and the American Society of Mohs Histotechnology over the last year. The majority of Mohs histotechs are not registered HT's or HTL's. At this time it is just under discussion with questionnaires. I would be interested to know if there is any resource available that covers the requirements for licensure in each state? Would knowledgeable persons from each state be willing to post what they know about the requirements in the states they reside and work in? Right now, Washington State has no requirements for licensure of histotechs, Mohs or otherwise. If people will email me I will compile what I receive and post a compilation if this has not already been done. Thank you. Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Monday, May 26, 2008 4:46 PM To: 'Brad Miller'; 'patsy ruegg'; 'MaryAnn Dixon'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH bulletin (In Action) I'm not an expert in the NY licensure, but I'll fill in some gaps from what I've learned from Histonets and talking with NY histotechs. The law that was passed in 2006 allows for Cytotechs, MLT's and MT's. Though HT/HTL were originally written into the licensure bill when it was first drafted over a decade ago, somewhere over time, HT and HTL were dropped from the NY bill. So as of right now, any histotech currently working in NY are grandfathered in, so they can continue to work as histotechs in NY. Any new histotech coming into the field in NY, or already experienced and moving to NY, must take all the classes to be a MT or a MLT, AND pass the MT/MLT exam. ASCP and NSH and the NY histotechs and the NY pathologists societies have been working to try to get an amendment to the law, recognizing HT/HTL as a separate tech category. However, once a law has been adopted, it becomes very hard to get the legislators to agree to change it. And can be difficult to get the wording correct (both of which are what is happening now). There are other states out there, working on licensure (Michigan, where I'm from, have been working on it for over 15 years). But it's up to us histotechs to remain aware of what other lab societies within our own states are working on, so histotechs don't get left out of a law. (The Michigan draft does list histotechs (HT and HTL), as well as PA's, cytogenetic technologists, EM techs, etc. However, we have 2 histotechs in our state who try to keep track of where the draft is - which legislatures are behind it, what the lobbyists are doing. It's being pushed by the med tech society in the state, but they (med tehc society) have been working with all the Michigan lab societies. The Michigan med techs are very aware of what happened in NY, and don't want it to happen here.) So, no, don't get out of the histotechnology. We need people like yourself, who are aware of the effects of state and national laws on our profession, and who are willing to look out for our field. And who would be willing to talk with cytotechs and med techs, and remind them that there are other lab professionals. For the latest from the NSH web page, go to: http://www.nsh.org/organizations.php3?action=printContentItem&orgid=111&type ID=1203&itemID=22168 Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brad Miller Sent: Monday, May 26, 2008 2:37 PM To: patsy ruegg; 'MaryAnn Dixon'; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] NSH bulletin (In Action) NYS Legislative Update - April 6, 2008 In the last few weeks there has been a lot of activity on the legislative front in NYS. As a recap, a law (Article 165) requiring licensing of all clinical laboratory personnel was passed by the NYS legislation and went into effect in September of 2006 (please visit http://www.op.nysed.gov/clp-cltlic.htm for specific details). Unfortunately, histologists did not have representation on the board that crafted the license nor did we have lobbyists working for our interests. As a result there is no language describing the scope of practice for histologists, no distinction between technician and technologist and no provision for academic training requirements (curriculum). ----- Original Message ----- From: "patsy ruegg" To: "'MaryAnn Dixon'" ; Sent: Sunday, May 25, 2008 10:05 AM Subject: RE: [Histonet] NSH bulletin (In Action) > Please elaborate! > > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. #215 > Aurora, CO 80045 > 720-859-4060 > fax 720-859-4110 > pruegg@ihctech.net > www.ihctech.net > www.ihcrg.org > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > MaryAnn Dixon > Sent: Tuesday, May 20, 2008 1:13 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] NSH bulletin (In Action) > > Hi Histonetters, > > Has anyone read the In Action NSH bulletin lately? Just another > example of the lack of recognition for the field of Histology. I am > new to the field of Histology. What has happened to those employed in > the NY labs? Are other states following by example? Should I get out > now before I am unemployed? > > MaryAnn Dixon > Biological Scientist > Anatomic Pathology > University of Florida Medical Center > 352-392-2235 ext 4517 > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG. > Version: 7.5.524 / Virus Database: 269.24.1/1464 - Release Date: > 5/24/2008 > 8:56 AM > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmiller <@t> physlab.com Fri May 30 06:08:14 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Fri May 30 06:07:00 2008 Subject: [Histonet] Immuno Controls In-Reply-To: References: Message-ID: <000601c8c245$750409b0$3d02a8c0@plab.local> I put control tissue on each slide I stain except the neg controls. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Happel, James F. Sent: Thursday, May 29, 2008 2:11 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Immuno Controls Good Day Histonetters! For immuno cases, how are you handling the control tissue question? Are you putting a piece of control tissue on each slide or are you using a control slide for each ab performed on a given day. We are using Ventana'e Benchmark for the lion's share of our immunos. Thanks! James Happel Massachusetts General Hospital The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From pieronelva01 <@t> bigpond.com Fri May 30 06:19:25 2008 From: pieronelva01 <@t> bigpond.com (Piero Nelva) Date: Fri May 30 06:19:32 2008 Subject: [Histonet] Immuno Controls References: Message-ID: <006101c8c247$04ee45d0$6875be7c@pentium4> We put one control section per slide, so that every slide has its own control. Piero Nelva Monash Medical Centre Melbourne Australia ----- Original Message ----- From: "Happel, James F." To: Sent: Friday, May 30, 2008 5:11 AM Subject: [Histonet] Immuno Controls Good Day Histonetters! For immuno cases, how are you handling the control tissue question? Are you putting a piece of control tissue on each slide or are you using a control slide for each ab performed on a given day. We are using Ventana'e Benchmark for the lion's share of our immunos. Thanks! James Happel Massachusetts General Hospital The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG. Version: 8.0.100 / Virus Database: 269.24.2/1471 - Release Date: 5/28/2008 5:33 PM From stevihay <@t> yahoo.com.hk Fri May 30 06:35:51 2008 From: stevihay <@t> yahoo.com.hk (ng man hay) Date: Fri May 30 06:36:00 2008 Subject: [Histonet] Strange staining pattern Message-ID: <13406.62496.qm@web52612.mail.re2.yahoo.com> Hi, I performed HMWCK on prostate biopsy but showing some strange result. This = sntibody will stain the basal cells of the prostate glands. For my result, = most of the glands are positive. However, some basal cells of the gland is = positive for the left side but negative for the right. Moreover, some cells= are strongly positive but some are weakly positive for the same lesion. Fo= r those weakly positive stained cells, they seemed to be dewax inadequately= and the counterstain is weaker than other cells for the same slides. Do anyone have any opinion on this? thanks!! Ivan=0A=0A=0A Yahoo! Mail=A8=E3=B3=C6=A4@=ACy=AA=BA=BA=F4=A4W=A6w=A5= =FE=ABO=C5@=A5\=AF=E0=A1A=BD=D0=ABe=A9=B9 http://hk.antispam.yahoo.com/ =A4= F=B8=D1=A7=F3=A6h=AC=DB=C3=F6=B8=EA=B0T! From gliuygao <@t> hotmail.com Fri May 30 07:51:05 2008 From: gliuygao <@t> hotmail.com (yan gao) Date: Fri May 30 07:51:14 2008 Subject: [Histonet] IGFII antibody Message-ID: Hi, Histonet. Does anyone know a good IGFII antibody working on human tissues or Monkey tissues? Thanks. Yan Gao Novartis _________________________________________________________________ Change the world with e-mail. Join the i?m Initiative from Microsoft. http://im.live.com/Messenger/IM/Join/Default.aspx?source=EML_WL_ChangeWorld From M.Walker <@t> hrsu.mrc.ac.uk Fri May 30 08:24:43 2008 From: M.Walker <@t> hrsu.mrc.ac.uk (Marion Walker) Date: Fri May 30 08:25:27 2008 Subject: [Histonet] Fluorescent Mounting Media [Scanned] Message-ID: <86F334797DC6524A99AD9DD8F23A8B500DDE1A@mailserv.hrsu.mrc.ac.uk> Dear All, We have just been told by our suppliers that Permafluor mounting media is no longer available and I was just wondering what other folks are using? Ideally we would like a mounting media that sets hard (no guddling around with nail varnish etc.) and that allows slides to be viewed long term. We have been known to look at slides up to a year after staining with no loss of signal. Any suggestions would be greatly appreciated. Thanks Marion Marion Walker Human Reproductive Sciences Unit Edinburgh Scotland UK From sotlak <@t> yahoo.gr Fri May 30 08:48:49 2008 From: sotlak <@t> yahoo.gr (sotiris lakis) Date: Fri May 30 08:48:57 2008 Subject: [Histonet] What is "IgG cut" Message-ID: <361304.15031.qm@web23001.mail.ird.yahoo.com> Hello to everyone. What is IgG cut? I only know it's about antibody clearing. Can it be done in any laboratory? Could somebody give me a hint? Thanks Sotiris Lakis Resident in Pathology Greece --------------------------------- ?????????????? Yahoo! ?????????? ?? ?????????? ???? ???? (spam); ?? Yahoo! Mail ???????? ??? ???????? ?????? ????????? ???? ??? ??????????? ????????? http://login.yahoo.com/config/mail?.intl=gr From mickie25 <@t> netzero.net Fri May 30 09:33:37 2008 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Fri May 30 09:33:33 2008 Subject: [Histonet] Immuno Controls In-Reply-To: <000601c8c245$750409b0$3d02a8c0@plab.local> References: <000601c8c245$750409b0$3d02a8c0@plab.local> Message-ID: Hi Histonetters, I would like to add my 2 cents worth. When I started IHC it was by hand and the tech was sure they had put primary Ab, etc on each slide. Then we went automated with a Ventana ES. We learned the hard way that dispensers may or may not dispense their contents and that this could vary from slide to slide on the same run! This is when we began to put a control slide on each positive patient slide for each antibody run on that patient and block. Machines make mistakes too. Thanks. Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Friday, May 30, 2008 4:08 AM To: 'Happel, James F.'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Immuno Controls I put control tissue on each slide I stain except the neg controls. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Happel, James F. Sent: Thursday, May 29, 2008 2:11 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Immuno Controls Good Day Histonetters! For immuno cases, how are you handling the control tissue question? Are you putting a piece of control tissue on each slide or are you using a control slide for each ab performed on a given day. We are using Ventana'e Benchmark for the lion's share of our immunos. Thanks! James Happel Massachusetts General Hospital The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SharonC <@t> celligent.net Fri May 30 09:49:15 2008 From: SharonC <@t> celligent.net (Sharon Campbell) Date: Fri May 30 09:46:08 2008 Subject: [Histonet] IHC Controls Message-ID: I would like to ask a question about putting control tissue on "unstained" slides. We occasionally receive unstained slides and need to run IHC or ISH on them. We are using the Ventana Benchmark and Benchmark XT. To save money we are wondering if it is ok to float control tissue onto the existing tissue slide. Is there a problem with carefully turning the slide so that the top (end with the writing) is dipped into the waterbath and the positive control floated onto the top of the glass portion? Thank you for your help. Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 (704) 549-8444 x100 sharonc@celligent.net From rjbuesa <@t> yahoo.com Fri May 30 09:47:09 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri May 30 09:47:18 2008 Subject: [Histonet] Immuno Controls In-Reply-To: Message-ID: <996939.51779.qm@web65705.mail.ac4.yahoo.com> And it is also true that some machines are more reliable than others and that we have to decide which to select. Ren? J. Mickie Johnson wrote: Hi Histonetters, I would like to add my 2 cents worth. When I started IHC it was by hand and the tech was sure they had put primary Ab, etc on each slide. Then we went automated with a Ventana ES. We learned the hard way that dispensers may or may not dispense their contents and that this could vary from slide to slide on the same run! This is when we began to put a control slide on each positive patient slide for each antibody run on that patient and block. Machines make mistakes too. Thanks. Best Regards, Mickie From LSebree <@t> uwhealth.org Fri May 30 09:49:45 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Fri May 30 09:49:49 2008 Subject: [Histonet] IHC Controls In-Reply-To: Message-ID: One of the reference labs we use does just that, fitting their positive control tissue wherever on the slide it will fit. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon Campbell Sent: Friday, May 30, 2008 9:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Controls I would like to ask a question about putting control tissue on "unstained" slides. We occasionally receive unstained slides and need to run IHC or ISH on them. We are using the Ventana Benchmark and Benchmark XT. To save money we are wondering if it is ok to float control tissue onto the existing tissue slide. Is there a problem with carefully turning the slide so that the top (end with the writing) is dipped into the waterbath and the positive control floated onto the top of the glass portion? Thank you for your help. Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 (704) 549-8444 x100 sharonc@celligent.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rcharles <@t> state.pa.us Fri May 30 09:53:59 2008 From: rcharles <@t> state.pa.us (Charles, Roger) Date: Fri May 30 09:54:11 2008 Subject: FW: [Histonet] Immuno Controls Message-ID: <12E4E17FEF6EBE4BAE95BEB3CDCB57000943BBEB@enhbgpri04.backup> Hello all, I've read many posts on this subject of people putting controls on each slide because machines do make mistakes. I do not do this; I use one slide as a control (which contains both negative and positive tissue) for each run or each different antibody. What I would like to know from those putting controls on each slide is if they ever seen one or more slides where the control did not work but yet controls on other slides from the same run and same antibody did. Convince me to change my ways. Thanks Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mickie Johnson Sent: Friday, May 30, 2008 10:34 AM To: 'Cheri Miller'; 'Happel, James F.'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Immuno Controls Hi Histonetters, I would like to add my 2 cents worth. When I started IHC it was by hand and the tech was sure they had put primary Ab, etc on each slide. Then we went automated with a Ventana ES. We learned the hard way that dispensers may or may not dispense their contents and that this could vary from slide to slide on the same run! This is when we began to put a control slide on each positive patient slide for each antibody run on that patient and block. Machines make mistakes too. Thanks. Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Friday, May 30, 2008 4:08 AM To: 'Happel, James F.'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Immuno Controls I put control tissue on each slide I stain except the neg controls. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Happel, James F. Sent: Thursday, May 29, 2008 2:11 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Immuno Controls Good Day Histonetters! For immuno cases, how are you handling the control tissue question? Are you putting a piece of control tissue on each slide or are you using a control slide for each ab performed on a given day. We are using Ventana'e Benchmark for the lion's share of our immunos. Thanks! James Happel Massachusetts General Hospital The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From japoteete <@t> saintfrancis.com Fri May 30 10:05:28 2008 From: japoteete <@t> saintfrancis.com (Poteete, Jacquie A.) Date: Fri May 30 10:08:16 2008 Subject: [Histonet] Immuno Controls In-Reply-To: <12E4E17FEF6EBE4BAE95BEB3CDCB57000943BBEB@enhbgpri04.backup> Message-ID: Yes, Roger, in fact I have seen this happen twice in the last week. One of my elderly instruments chose to "forget" to dispense reagent both times. I've also seen instruments dispense fully, partially, or not at all during the same run. Yes, service calls were generated, and everything was repeated. Of course, the instrument's "brother" started its run by itself without us pushing the "START" button, but that's another story.....and new instrumets are on order. Jacquie Poteete MT(ASCP)QIHC Lead Technologist, IHC Laboratory Saint Francis Hospital, Tulsa, OK japoteete@saintfrancis.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles, Roger Sent: Friday, May 30, 2008 9:54 AM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] Immuno Controls Hello all, I've read many posts on this subject of people putting controls on each slide because machines do make mistakes. I do not do this; I use one slide as a control (which contains both negative and positive tissue) for each run or each different antibody. What I would like to know from those putting controls on each slide is if they ever seen one or more slides where the control did not work but yet controls on other slides from the same run and same antibody did. Convince me to change my ways. Thanks Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mickie Johnson Sent: Friday, May 30, 2008 10:34 AM To: 'Cheri Miller'; 'Happel, James F.'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Immuno Controls Hi Histonetters, I would like to add my 2 cents worth. When I started IHC it was by hand and the tech was sure they had put primary Ab, etc on each slide. Then we went automated with a Ventana ES. We learned the hard way that dispensers may or may not dispense their contents and that this could vary from slide to slide on the same run! This is when we began to put a control slide on each positive patient slide for each antibody run on that patient and block. Machines make mistakes too. Thanks. Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Friday, May 30, 2008 4:08 AM To: 'Happel, James F.'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Immuno Controls I put control tissue on each slide I stain except the neg controls. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Happel, James F. Sent: Thursday, May 29, 2008 2:11 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Immuno Controls Good Day Histonetters! For immuno cases, how are you handling the control tissue question? Are you putting a piece of control tissue on each slide or are you using a control slide for each ab performed on a given day. We are using Ventana'e Benchmark for the lion's share of our immunos. Thanks! James Happel Massachusetts General Hospital The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dermpathsy <@t> gmail.com Fri May 30 10:12:25 2008 From: dermpathsy <@t> gmail.com (Sate Hamza) Date: Fri May 30 10:12:36 2008 Subject: [Histonet] Immuno Controls In-Reply-To: References: Message-ID: <8854ff80805300812je7b4ce6mcd6aa0cce592b6f7@mail.gmail.com> Greetings everyone .. I am a pathologist and I do not feel I miss much by not having a control on each slide. We use Dako immunostainers. The reality is that there are internal positive controls for many antibodies on many of the tissues that we test daily for diagnostic purposes .. Evaluation of the staining pattern (or lack thereof) with some antibody helps me know in most instances whether to believe the negative tissue immunoreaction or not ... Now I know that does not apply to all antibodies, but it does for most of the ones that we use daily .. So I am not sure that the extra effort and cost to have a separate positive control tissue on each and every slide for each and every antibody is justifiable.. Sate On Thu, May 29, 2008 at 4:29 PM, Dana Spears wrote: > Histonetters, > > In my opinion, it is always better for patient safety to have the control > and the patient tissue on the same slide - no matter what platform you are > running (or even if you were running them manually!). I've used BioGenex, > DAKO, Ventana, and manual methods and I feel it is better for the patient > AND cheaper if the control is on the same slide. No matter how you are > running your IHCs, there is the slight possibility that the instrument could > falter or you could forget to apply a reagent to a particular slide - better > to have the control on there and you KNOW it worked. Sure, there is the > argument that you go through more control blocks, but if you align your > chuck every time and are careful, that additional tissue is a small price to > pay for better patient care!!! > > > > Dana Spears, HTL(ASCP) > Anatomic Pathology Manager > Methodist Medical Center > (309) 672-4930 (office) > (309) 255-7214 (cell) > dspears@mmci.org > > >>> "Mark A Burton" 5/29/2008 3:15 PM >>> > > I'm sure this discussion must be in the archives but my understanding of > how > the Ventana Benchmark works is that there is a plunger that presses on a > dispenser bottle to deliver the antibodies and reagents to the slide. If > the > dispenser bottle is not primed or if there is some type of interference > than > you would not get any antibody or reagent delivered to your slide. Without > a > control on each slide, there is no way to know. Is this a legitimate > argument? or is the amount of time and tissue needed to accomplish this too > costly? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J > Buesa > Sent: Thursday, May 29, 2008 3:30 PM > To: Happel, James F.; Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Immuno Controls > > I used the DAKO autostainer and the cases' sections were placed in slides > already containing a tissue control section in a way that both were > "stained" simultaneously. > I only used one single control for a whole series of cases when doing > Her2neu, or for research studies for our residents. > Ren? J. > > -- Sate Hamza, MD, FRCPC Dermatopathologist Winnipeg, Canada From Terri.Brown <@t> Northside.com Fri May 30 10:31:13 2008 From: Terri.Brown <@t> Northside.com (Terri Brown) Date: Fri May 30 10:31:39 2008 Subject: [Histonet] Job Opening Message-ID: <8CEB6DA1A3F35743800669D4CFE21F7D03F90005@NSMXMS04.northside.local> Northside Hospital a busy 444 bed, not for profit hospital in the popular Sandy Springs area of Atlanta, has an immediate opening for a Histotechnologist. Renowned for its expertise in women's services, Northside ranks first in the nation among community hospitals in the number of babies delivered. Northside is accredited by Joint Commission, (JCAHO), CAP, and named as Atlanta's most preferred hospital for over all health care services in the National Healthcare Market Guide Survey. Northside offers great benefits, competitive salaries, and state of the art equipment. Call Royce Roberts, Human Resources at 404-851-8748 for further information. Terri H. Brown Pathology Laboratory Manager Northside Hospital Office: 404-845-5423 Fax: 404-851-6400 terri.brown@northside.com CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. From mwich <@t> 7thwavelabs.com Fri May 30 10:39:56 2008 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Fri May 30 10:40:00 2008 Subject: [Histonet] MMA de-plasticizing Message-ID: <62A8156F8071C8439080D626DF8C33A602E40C@wave-mail.7thwave.local> Is there anyone out there doing MMA on un-decalcified bone? I'm wondering if there is any way to de-plasticize without putting the slides in heated xylene--a potentially explosive situation which technically would require explosion proof oven, hood, clothing, etc. I know that the flash point of xylene is quite low (26.1?C, I think, which is barely above room temperature). Is there a way around this safety issue? This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From tjasper <@t> copc.net Fri May 30 10:46:33 2008 From: tjasper <@t> copc.net (Thomas Jasper) Date: Fri May 30 10:46:38 2008 Subject: FW: [Histonet] Immuno Controls Message-ID: <90354A475B420441B2A0396E5008D4965E20CE@copc-sbs.COPC.local> -----Original Message----- From: Thomas Jasper Sent: Friday, May 30, 2008 8:46 AM To: 'Charles, Roger' Subject: RE: [Histonet] Immuno Controls Hey Roger, Have been following this thread and your post has convinced me to finally weigh-in. I'm a little unclear when you say you put both a positive and a negative on one slide. When you say per run or per antibody, this assumes the per run to be one antibody only? The negative should be from your target (patient), I say target as with animal work I'm not sure if it's research or individual vet diagnostic cases. Also a slide designated as a negative should get all reagents sans the antibody/antibodies that are being run. If you've got both on one slide this seems impossible to me. The positive control must receive the antibody!? My understanding of running a negative is to demonstrate that the reagents being run are not contaminated or are going to cause any sort of false positive. Therefore, what is seen as positive staining on the target (patient) slides is a true positive. I also don't have a problem with folks putting a positive control on each slide, however this can be cumbersome and/or not an option with large sections of tissue. I believe; as long as a known positive control, is run per antibody, per run, whether it's on the same slide as the target (patient) tissue or on a separate slide, and that known positive lights up, it's all good. I do not worry too much about machine malfunction. If for some reason you are seeing less than desirable staining or unexpected results, machine malfunction is only one path of investigation for troubleshooting. Prior to IHC being automated there were a lot more problems. What automation has done has given us greater consistency and helped to eliminate variables, thus improving the staining overall. This also narrows down any necessary troubleshooting with problematic staining. I'm sure there are folks out there that will disagree with me. That's ok, if what people are doing works for them, their scope of service, application etc., so be it. I know you are not in the clinical world of human histology, but for us this is the most efficient and successful approach to meeting our needs for IHC. Hope this helps. Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles, Roger Sent: Friday, May 30, 2008 7:54 AM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] Immuno Controls Hello all, I've read many posts on this subject of people putting controls on each slide because machines do make mistakes. I do not do this; I use one slide as a control (which contains both negative and positive tissue) for each run or each different antibody. What I would like to know from those putting controls on each slide is if they ever seen one or more slides where the control did not work but yet controls on other slides from the same run and same antibody did. Convince me to change my ways. Thanks Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mickie Johnson Sent: Friday, May 30, 2008 10:34 AM To: 'Cheri Miller'; 'Happel, James F.'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Immuno Controls Hi Histonetters, I would like to add my 2 cents worth. When I started IHC it was by hand and the tech was sure they had put primary Ab, etc on each slide. Then we went automated with a Ventana ES. We learned the hard way that dispensers may or may not dispense their contents and that this could vary from slide to slide on the same run! This is when we began to put a control slide on each positive patient slide for each antibody run on that patient and block. Machines make mistakes too. Thanks. Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Friday, May 30, 2008 4:08 AM To: 'Happel, James F.'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Immuno Controls I put control tissue on each slide I stain except the neg controls. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Happel, James F. Sent: Thursday, May 29, 2008 2:11 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Immuno Controls Good Day Histonetters! For immuno cases, how are you handling the control tissue question? Are you putting a piece of control tissue on each slide or are you using a control slide for each ab performed on a given day. We are using Ventana'e Benchmark for the lion's share of our immunos. Thanks! James Happel Massachusetts General Hospital The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mburton1 <@t> bu.edu Fri May 30 10:56:59 2008 From: mburton1 <@t> bu.edu (Mark A Burton) Date: Fri May 30 10:57:28 2008 Subject: [Histonet] Immuno Controls In-Reply-To: References: <12E4E17FEF6EBE4BAE95BEB3CDCB57000943BBEB@enhbgpri04.backup> Message-ID: <002f01c8c26d$cc42e8e0$64c8baa0$@edu> If your instrument is going to "forget" to dispense even once in its lifetime, does that justify not using a control on each slide? Maybe I'm being a little dramatic, but I wouldn't want my tissue on the slide that day. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Poteete, Jacquie A. Sent: Friday, May 30, 2008 11:05 AM To: Charles, Roger; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Immuno Controls Yes, Roger, in fact I have seen this happen twice in the last week. One of my elderly instruments chose to "forget" to dispense reagent both times. I've also seen instruments dispense fully, partially, or not at all during the same run. Yes, service calls were generated, and everything was repeated. Of course, the instrument's "brother" started its run by itself without us pushing the "START" button, but that's another story.....and new instrumets are on order. Jacquie Poteete MT(ASCP)QIHC Lead Technologist, IHC Laboratory Saint Francis Hospital, Tulsa, OK japoteete@saintfrancis.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles, Roger Sent: Friday, May 30, 2008 9:54 AM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] Immuno Controls Hello all, I've read many posts on this subject of people putting controls on each slide because machines do make mistakes. I do not do this; I use one slide as a control (which contains both negative and positive tissue) for each run or each different antibody. What I would like to know from those putting controls on each slide is if they ever seen one or more slides where the control did not work but yet controls on other slides from the same run and same antibody did. Convince me to change my ways. Thanks Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mickie Johnson Sent: Friday, May 30, 2008 10:34 AM To: 'Cheri Miller'; 'Happel, James F.'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Immuno Controls Hi Histonetters, I would like to add my 2 cents worth. When I started IHC it was by hand and the tech was sure they had put primary Ab, etc on each slide. Then we went automated with a Ventana ES. We learned the hard way that dispensers may or may not dispense their contents and that this could vary from slide to slide on the same run! This is when we began to put a control slide on each positive patient slide for each antibody run on that patient and block. Machines make mistakes too. Thanks. Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Friday, May 30, 2008 4:08 AM To: 'Happel, James F.'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Immuno Controls I put control tissue on each slide I stain except the neg controls. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Happel, James F. Sent: Thursday, May 29, 2008 2:11 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Immuno Controls Good Day Histonetters! For immuno cases, how are you handling the control tissue question? Are you putting a piece of control tissue on each slide or are you using a control slide for each ab performed on a given day. We are using Ventana'e Benchmark for the lion's share of our immunos. Thanks! James Happel Massachusetts General Hospital The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mwstarbu <@t> mdanderson.org Fri May 30 11:23:47 2008 From: mwstarbu <@t> mdanderson.org (mwstarbu@mdanderson.org) Date: Fri May 30 11:24:12 2008 Subject: [Histonet] MMA de-plasticizing Message-ID: Hi Michele, We use 2-methoxyethyl acetate a.k.a. 1-acetoxy-2-methoxyethane (AME). We do 2 changes 20 minutes each at room temperature under the hood. This works very well for us. Good luck, Mike Michele Wich Sent by: 05/30/2008 10:39 AM To: cc: Subject: [Histonet] MMA de-plasticizing Is there anyone out there doing MMA on un-decalcified bone? I'm wondering if there is any way to de-plasticize without putting the slides in heated xylene--a potentially explosive situation which technically would require explosion proof oven, hood, clothing, etc. I know that the flash point of xylene is quite low (26.1?C, I think, which is barely above room temperature). Is there a way around this safety issue? This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri May 30 11:24:25 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri May 30 11:24:30 2008 Subject: FW: [Histonet] Immuno Controls In-Reply-To: <12E4E17FEF6EBE4BAE95BEB3CDCB57000943BBEB@enhbgpri04.backup> Message-ID: <757371.22139.qm@web65714.mail.ac4.yahoo.com> I don't think that it is the role of HistoNet of convincing anybody to do or stop doing something. Here a "full package" of opinions and experiences is presented and it is the individual who will have to "digest" what is posted, pros and cons, and act according with the proper reasoning on each issue. My question to you could be: if a case turned out negative in one of those runs with a sole (+) control, was it negative because it was really negative or because that particular section of that case did not receive all the reagents? You cannot answer that question with your method. Ren? J. "Charles, Roger" wrote: Hello all, I've read many posts on this subject of people putting controls on each slide because machines do make mistakes. I do not do this; I use one slide as a control (which contains both negative and positive tissue) for each run or each different antibody. What I would like to know from those putting controls on each slide is if they ever seen one or more slides where the control did not work but yet controls on other slides from the same run and same antibody did. Convince me to change my ways. Thanks Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mickie Johnson Sent: Friday, May 30, 2008 10:34 AM To: 'Cheri Miller'; 'Happel, James F.'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Immuno Controls Hi Histonetters, I would like to add my 2 cents worth. When I started IHC it was by hand and the tech was sure they had put primary Ab, etc on each slide. Then we went automated with a Ventana ES. We learned the hard way that dispensers may or may not dispense their contents and that this could vary from slide to slide on the same run! This is when we began to put a control slide on each positive patient slide for each antibody run on that patient and block. Machines make mistakes too. Thanks. Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Friday, May 30, 2008 4:08 AM To: 'Happel, James F.'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Immuno Controls I put control tissue on each slide I stain except the neg controls. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Happel, James F. Sent: Thursday, May 29, 2008 2:11 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Immuno Controls Good Day Histonetters! For immuno cases, how are you handling the control tissue question? Are you putting a piece of control tissue on each slide or are you using a control slide for each ab performed on a given day. We are using Ventana'e Benchmark for the lion's share of our immunos. Thanks! James Happel Massachusetts General Hospital The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Fri May 30 11:29:44 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Fri May 30 11:29:47 2008 Subject: [Histonet] MMA de-plasticizing References: <62A8156F8071C8439080D626DF8C33A602E40C@wave-mail.7thwave.local> Message-ID: <002401c8c272$5e7deb70$6401a8c0@DHXTS541> Michele, Lots of people doing MMA embedded bone and with the exact safety devices and other precautions you described when working with the methyl methacrylate monomers, plasticizers, catalysts, and solvents. These are also highly toxic and explosive - as much or more so than warm xylene. There have been some other solvents described in Histonet Archives besides xylene. The xylene does not have to be hot, just 37C for approx 15 to 20 minutes according to Neil Hand (expert at doing this). However, his sections were very thin, approx 2 um (you did not say how thick your sections are going to be?) If your sections are thicker than Hands, then increase time and with extra changes of xylene to ensure MMA removal. A water bath to hold very tightly sealed staining jars of xylene is advisable to avoid any water contamination in with the xylene/sections. Remember that people add heat to tissue processing stations on automated tissue processor - including the xylene stations and that temperature will be as much as 40C, and possibly slightly higher. As long as you are in a hood, things should go well. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT ---- Original Message ----- From: "Michele Wich" To: Sent: Friday, May 30, 2008 9:39 AM Subject: [Histonet] MMA de-plasticizing Is there anyone out there doing MMA on un-decalcified bone? I'm wondering if there is any way to de-plasticize without putting the slides in heated xylene--a potentially explosive situation which technically would require explosion proof oven, hood, clothing, etc. I know that the flash point of xylene is quite low (26.1?C, I think, which is barely above room temperature). Is there a way around this safety issue? This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From japoteete <@t> saintfrancis.com Fri May 30 11:29:37 2008 From: japoteete <@t> saintfrancis.com (Poteete, Jacquie A.) Date: Fri May 30 11:29:52 2008 Subject: [Histonet] Immuno Controls In-Reply-To: <002f01c8c26d$cc42e8e0$64c8baa0$@edu> Message-ID: That's why I use controls on all my slides. I treat every slide I run like it IS my tissue, so yes, I use lots of controls. Our pathologists also pay very close attention to internal controls, and have always done so. It works for us, but each institution should decide what works best for them. Jacquie Poteete MT(ASCP)QIHC Lead Technologist, IHC Laboratory Saint Francis Hospital, Tulsa, OK japoteete@saintfrancis.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark A Burton Sent: Friday, May 30, 2008 10:57 AM To: 'Charles, Roger' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Immuno Controls If your instrument is going to "forget" to dispense even once in its lifetime, does that justify not using a control on each slide? Maybe I'm being a little dramatic, but I wouldn't want my tissue on the slide that day. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Poteete, Jacquie A. Sent: Friday, May 30, 2008 11:05 AM To: Charles, Roger; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Immuno Controls Yes, Roger, in fact I have seen this happen twice in the last week. One of my elderly instruments chose to "forget" to dispense reagent both times. I've also seen instruments dispense fully, partially, or not at all during the same run. Yes, service calls were generated, and everything was repeated. Of course, the instrument's "brother" started its run by itself without us pushing the "START" button, but that's another story.....and new instrumets are on order. Jacquie Poteete MT(ASCP)QIHC Lead Technologist, IHC Laboratory Saint Francis Hospital, Tulsa, OK japoteete@saintfrancis.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles, Roger Sent: Friday, May 30, 2008 9:54 AM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] Immuno Controls Hello all, I've read many posts on this subject of people putting controls on each slide because machines do make mistakes. I do not do this; I use one slide as a control (which contains both negative and positive tissue) for each run or each different antibody. What I would like to know from those putting controls on each slide is if they ever seen one or more slides where the control did not work but yet controls on other slides from the same run and same antibody did. Convince me to change my ways. Thanks Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mickie Johnson Sent: Friday, May 30, 2008 10:34 AM To: 'Cheri Miller'; 'Happel, James F.'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Immuno Controls Hi Histonetters, I would like to add my 2 cents worth. When I started IHC it was by hand and the tech was sure they had put primary Ab, etc on each slide. Then we went automated with a Ventana ES. We learned the hard way that dispensers may or may not dispense their contents and that this could vary from slide to slide on the same run! This is when we began to put a control slide on each positive patient slide for each antibody run on that patient and block. Machines make mistakes too. Thanks. Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Friday, May 30, 2008 4:08 AM To: 'Happel, James F.'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Immuno Controls I put control tissue on each slide I stain except the neg controls. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Happel, James F. Sent: Thursday, May 29, 2008 2:11 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Immuno Controls Good Day Histonetters! For immuno cases, how are you handling the control tissue question? Are you putting a piece of control tissue on each slide or are you using a control slide for each ab performed on a given day. We are using Ventana'e Benchmark for the lion's share of our immunos. Thanks! James Happel Massachusetts General Hospital The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Fri May 30 12:29:21 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Fri May 30 12:29:32 2008 Subject: [Histonet] Hydroxyprobe-1 or Hydroxyprobe Omni kits for detecting hypoxia Message-ID: <005201c8c27a$b46a1970$6401a8c0@DHXTS541> Is anyone doing the staining for hypoxia using these kits (Hydroxyprobe-1 or Hydroxyprobe Omni)? I have a researcher (his laboratory personnel is not experienced with immunostaining, and not sure he has done it himself) wanting set up immunostaining on murine lung showing hypoxia using one of these kits. One avoids mouse on mouse staining issues (Omni contains polyclonal rabbit primary) while the other kit has success with using the mouse monoclonal primary (Hydroxyprobe-1). For a lab starting out with immunhistochemical staining, avoiding the mouse on mouse issue may be a good introduction to IHC, but maybe the Omni Rabbit polyclonal kit is not as ideal. They can certainly learn the ropes here. Questions: 1. Which kit do you prefer? 2. Fluorescence or chromogenic method? With which fixation NBF or frozen sections fixed with acetone? 3. In their website, they cite a protocol by Raleigh et al, where they routinely use CSA (DAKO) for mouse antibodies for clinical samples. Is anyone using CSA as they did? We would like to avoid this if possible, and even use frozen section for enzyme IHC chromogenic methods. 4. Since mouse lung is the target tissue, we would like to use alkaline phosphatase instead of HRP enzyme method? Any comments? Our lung experts prefer alk phos over HRP due to high background issues with peroxidase methods in the past. Any comments are welcome Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT From kmerriam2003 <@t> yahoo.com Fri May 30 12:41:10 2008 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Fri May 30 12:41:15 2008 Subject: [Histonet] FISH experts, I need your help! Message-ID: <108914.19317.qm@web50307.mail.re2.yahoo.com> Hello, I am currently working on a FISH project (not?the?swimming kind) and I need some help. I have the FISH procedure working beautifully on cytospin preps of cultured cells and am now trying to get the same probe to work in FFPE?archival human tumors. I am using a DAKO probe (dual color, DNA probe, split signal) and the DAKO Histology FISH kit.? Here are the conditions that work?on the cytospins (after fixation): 1. denature for 5 minutes @ 84C 2. hybridize overnight @ 43C 3. stringency wash @ 63C Now, I am going with the assumption that the above conditions will not change for FFPE tissue (although I am not sure that this is true) and I am trying to work out the pretreatment conditions (to expose the nuclei).? I am using the reagents that are in the DAKO Histology FISH kit and I am pretreating as follows (as stated in the DAKO kit protocol): 1. heat in 99C waterbath for 10 mintues with DAKO pretreatment buffer (MES) 2. digest in?DAKO pepsin for 5-60 minutes (I have tried several different?incubation times) @ 37C What I am seeing is a bunch of "dots", both red and green and they all appear to be colocalized (not all of them should be colocalized), and?these "dots" don't all appear to be specifically in the nuclei, so I am guessing it is either autofluorescence or non-specific staining, but I am not sure. I am wondering if I should try some different types of preconditions (I have read many journal articles on FISH and there are a million different ways that people pretreat their FFPE tissues) or should I?start playing around with my denature, hybridization and/or stringency conditions. Any words of wisdom out there? Kim ?Kim Merriam, MA, HT(ASCP) Cambridge, MA From pkromund <@t> gundluth.org Fri May 30 12:51:06 2008 From: pkromund <@t> gundluth.org (pkromund@gundluth.org) Date: Fri May 30 12:51:49 2008 Subject: [Histonet] Immuno Controls In-Reply-To: Message-ID: I agree with this completely & this is the practice in our lab. Pam "Dana Spears" To Sent by: "Mark A Burton" , histonet-bounces@ lists.utsouthwest cc ern.edu Subject RE: [Histonet] Immuno Controls 05/29/2008 04:29 PM Histonetters, In my opinion, it is always better for patient safety to have the control and the patient tissue on the same slide - no matter what platform you are running (or even if you were running them manually!). I've used BioGenex, DAKO, Ventana, and manual methods and I feel it is better for the patient AND cheaper if the control is on the same slide. No matter how you are running your IHCs, there is the slight possibility that the instrument could falter or you could forget to apply a reagent to a particular slide - better to have the control on there and you KNOW it worked. Sure, there is the argument that you go through more control blocks, but if you align your chuck every time and are careful, that additional tissue is a small price to pay for better patient care!!! Dana Spears, HTL(ASCP) Anatomic Pathology Manager Methodist Medical Center (309) 672-4930 (office) (309) 255-7214 (cell) dspears@mmci.org >>> "Mark A Burton" 5/29/2008 3:15 PM >>> I'm sure this discussion must be in the archives but my understanding of how the Ventana Benchmark works is that there is a plunger that presses on a dispenser bottle to deliver the antibodies and reagents to the slide. If the dispenser bottle is not primed or if there is some type of interference than you would not get any antibody or reagent delivered to your slide. Without a control on each slide, there is no way to know. Is this a legitimate argument? or is the amount of time and tissue needed to accomplish this too costly? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, May 29, 2008 3:30 PM To: Happel, James F.; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Immuno Controls I used the DAKO autostainer and the cases' sections were placed in slides already containing a tissue control section in a way that both were "stained" simultaneously. I only used one single control for a whole series of cases when doing Her2neu, or for research studies for our residents. Ren? J. "Happel, James F." wrote: _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE: This message is a PRIVATE communication. This e-mail may contain confidential or proprietary information that may be considered legally privileged. It is intended only for the named recipient(s). If an addressing or transmission error has misdirected the e-mail, please notify the author by replying to this message. If you are not the named recipient, you are not authorized to use, disclose, distribute, copy, print, or rely on this e-mail, and should immediately delete it from your computer system. Thank you for your assistance with this matter. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From octavio109 <@t> hotmail.com Fri May 30 14:01:43 2008 From: octavio109 <@t> hotmail.com (Corinthia D. Emanuel) Date: Fri May 30 14:01:47 2008 Subject: [Histonet] FW: Histotechs needed in Atlanta, Ga In-Reply-To: References: Message-ID: Fax resume to 770-381-6451 for immediate consideration. Sign on bonus of $1000 offered! Experienced ONLY in cutting, staining, grossing and embedding, please! Certification preferred, but not required. Full-time and part-time postions available. All shifts. _________________________________________________________________ Give to a good cause with every e-mail. Join the i?m Initiative from Microsoft. http://im.live.com/Messenger/IM/Join/Default.aspx?souce=EML_WL_ GoodCause From detmar <@t> mshri.on.ca Fri May 30 14:26:26 2008 From: detmar <@t> mshri.on.ca (Jacqui Detmar) Date: Fri May 30 14:26:45 2008 Subject: [Histonet] Hydroxyprobe-1 or Hydroxyprobe Omni kits for detectinghypoxia In-Reply-To: <005201c8c27a$b46a1970$6401a8c0@DHXTS541> References: <005201c8c27a$b46a1970$6401a8c0@DHXTS541> Message-ID: Hi Gail. We have used hypoxyprobe on mouse placentae and have seen increased staining with one of our knockout mouse lines having defective placental vasculature (compared with wildtype placentae). We used the rabbit polyclonal kit and it worked nicely, so your researcher should not have a problem. Additionally, I have started some Hif1-alpha IHCs on mouse placental sections and I am getting a similar staining pattern to that seen with hypoxyprobe, so there does seem to be some biological validity to the technique (although I need to keep tweaking this antibody). This is an important fact to consider b/c I know when my labmate presented this work, she was harassed by the audience regarding the validity of the results. I'm sure it'll be no different upon manuscript submission and review. Anyway, while your researcher may need to provide a qualifier for the first set of experiments, subsequent experiments should not require this (if results are repeatable) and the hypoxyprobe technique is *so* much easier....I recommend it. Jacqui Jacqui Detmar, Post-doctoral Fellow Samuel Lunenfeld Research Institute, room 876 Mount Sinai Hospital 600 University Avenue Toronto, ON, Canada M5G 1X5 phone: 416-586-4800 x2451/x2290 fax: 416-586-8588 email: detmar@mshri.on.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Friday, May 30, 2008 1:29 PM To: Histonet Subject: [Histonet] Hydroxyprobe-1 or Hydroxyprobe Omni kits for detectinghypoxia Is anyone doing the staining for hypoxia using these kits (Hydroxyprobe-1 or Hydroxyprobe Omni)? I have a researcher (his laboratory personnel is not experienced with immunostaining, and not sure he has done it himself) wanting set up immunostaining on murine lung showing hypoxia using one of these kits. One avoids mouse on mouse staining issues (Omni contains polyclonal rabbit primary) while the other kit has success with using the mouse monoclonal primary (Hydroxyprobe-1). For a lab starting out with immunhistochemical staining, avoiding the mouse on mouse issue may be a good introduction to IHC, but maybe the Omni Rabbit polyclonal kit is not as ideal. They can certainly learn the ropes here. Questions: 1. Which kit do you prefer? 2. Fluorescence or chromogenic method? With which fixation NBF or frozen sections fixed with acetone? 3. In their website, they cite a protocol by Raleigh et al, where they routinely use CSA (DAKO) for mouse antibodies for clinical samples. Is anyone using CSA as they did? We would like to avoid this if possible, and even use frozen section for enzyme IHC chromogenic methods. 4. Since mouse lung is the target tissue, we would like to use alkaline phosphatase instead of HRP enzyme method? Any comments? Our lung experts prefer alk phos over HRP due to high background issues with peroxidase methods in the past. Any comments are welcome Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gagnone <@t> KGH.KARI.NET Fri May 30 14:44:37 2008 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Fri May 30 14:44:43 2008 Subject: [Histonet] Immuno Controls Message-ID: I would tend to agree that the immuno control issue is all about finding the balance between technical adequacy, pathologist confidence, economics and logistics. We are constantly trying to achieve this balance, by fine-tuning our control requirements accordingly. Often, we'll receive a request for marker(s) for more than one block of a case. We do not pick up a section from each block on a control. One control for the case (usually on a slide with one of the patient sections) is adequate for us. Taking that one step further, if two or three cases are on a run (Ventana BenchMark XT), for the same marker(s), we require only one control per marker per run. The one control per marker per pathologist idea was done away with long ago, they can share controls! Control and patient section on the same slide halves reagent costs, number of slides to coverslip and store, and allows more cases to tested per run. One exception being if unstained slides were cut on a small biopsy, we have to stain these and the control slide separately, as the tissue of interest may not be in the block after a deeper level was cut for routine staining. If we have any suspicion that an instrument or dispenser error has led to a false negative, that test is repeated. As Sate said, the internal positive controls and staining patterns are well-known to IHC techs and pathologists. We assess our positive controls before sending cases to the pathologists, although this may not be done in all centres,in which case the pathologist alone performs this function. Have a great weekend, Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada From amosbrooks <@t> gmail.com Fri May 30 22:06:19 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri May 30 22:06:24 2008 Subject: [Histonet] Immuno Controls Message-ID: <582736990805302006u1599e465r978b439adbd6f8c2@mail.gmail.com> Hi, Regaurding IHC controls, I agree that having a control on each slide is great. So is having an isotype negative for each one. As is having a non positive tissue labeled the same as the positive. The control on the same slide can often be missed by the drop zones of many automated stainers, so it might be good to have a couple on each slide on top and on the bottom, probably positive and negative too, just to be sure. One could, perhaps use a microarray on each slide. We can really spin our wheels on the subject of control tissues. There are good reasons for all these controls, but we have to be reasonable about how much effort goes into them as opposed to how much information you can get out of it. Running 5 control slides for each actual test slide, and not being able to tell the test from the control on the same slide is counter productive. Then again so is running just one slide and not knowing if it worked or not. We need to be responsible enough to anticipate where the problems are most likely to occur and run controls for these situations, working with doctors that actually care to look at the controls. Some actually don't even care about positive controls much the less negative! Ultimately it is their call to make and they need to be confident in your dilligence, but also know you aren't wasting time and money on controlling too many things that they may deem irrelevant. It is a balancing act. Amos Broooks From trathborne <@t> somerset-healthcare.com Sat May 31 11:47:38 2008 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Sat May 31 11:48:03 2008 Subject: [Histonet] Embedding centers and Hematoxylin Message-ID: I have a few questions for everyone. 1) How many years do you get from your embedding centers, and what make are they? 2) What type of hematoxylin does everyone use for their H&E's? In general, do you prefer a progressive or regressive stain? Thanks in advance for your replies. Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. From rjbuesa <@t> yahoo.com Sat May 31 12:54:25 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat May 31 12:54:28 2008 Subject: [Histonet] Embedding centers and Hematoxylin In-Reply-To: Message-ID: <712185.2914.qm@web65706.mail.ac4.yahoo.com> My 2 embedding centers were in operation from 1985 until I retired (2002) when they were still working. Maker: TissueTek I always used regressive hematoxylin from Richard Allan. Ren? J. "Rathborne, Toni" wrote: I have a few questions for everyone. 1) How many years do you get from your embedding centers, and what make are they? 2) What type of hematoxylin does everyone use for their H&E's? In general, do you prefer a progressive or regressive stain? Thanks in advance for your replies. Toni From JWeems <@t> sjha.org Sat May 31 12:55:53 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Sat May 31 12:56:19 2008 Subject: [Histonet] Embedding centers and Hematoxylin In-Reply-To: References: Message-ID: <982A0A9461F9BF438C7B19A6E425A3831A0CC5@ITSSSXM01V6.one.ads.che.org> We use Tissue Tek from Sakura. We just replaced one we had since the 70's. The old ones lasted forever, but I don't know how the new ones will do. We use Richard Allen Hematoxylin 7211, which is most nearly like the old Harris hematoxylin that I have found. We use it regressivly and get a nice bright stain without blue goblet cells. Best wishes, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Saturday, May 31, 2008 12:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Embedding centers and Hematoxylin I have a few questions for everyone. 1) How many years do you get from your embedding centers, and what make are they? 2) What type of hematoxylin does everyone use for their H&E's? In general, do you prefer a progressive or regressive stain? Thanks in advance for your replies. Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From contact <@t> excaliburpathology.com Sat May 31 13:56:01 2008 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Sat May 31 13:56:04 2008 Subject: [Histonet] Embedding centers and Hematoxylin Message-ID: <547692.87784.qm@web50112.mail.re2.yahoo.com> Ditto on the old?Tissue Teks. I still have one as a backup.?A newer Tissue Tek (1990s) waiting its turn and?Reichert Jung (1990s) in use now. Another old Tissue Tek?I worked with from 1979 until 2004. I think the new girls just wanted a new one and tossed it. I use Statlab Gill III regressively. ?Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 631 N. Broadway Ave. Moore, OK 73160 405-570-6679 cell 405-759-3953 lab contact@excaliburpathology.com www.excaliburpathology.com