From mickie25 <@t> netzero.net Sat Mar 1 11:15:00 2008 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Sat Mar 1 11:15:10 2008 Subject: [Histonet] rapid freeze containers In-Reply-To: <000001c87afc$df974840$3d02a8c0@plab.local> References: <000001c87afc$df974840$3d02a8c0@plab.local> Message-ID: This would be very interesting for Mohs techs too. We use a lot of Freeze Spray. Would people mind replying to 'All' so we can all get a perspective on this? Thanks! I have never seen a need for or a requirement for disposing of these as hazardous waste when empty. Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Friday, February 29, 2008 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] rapid freeze containers How is everyone disposing of their rapid freeze aerosol cans when empty? It is considered hazardous waste here in Omaha, not bio hazard but hazardous in the terms that the can is under pressure. We can purchase a can puncturer for 1000.00 but that seems steep. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alonso.martinezcanabal <@t> utoronto.ca Sat Mar 1 22:37:48 2008 From: alonso.martinezcanabal <@t> utoronto.ca (alonso.martinezcanabal@utoronto.ca) Date: Sat Mar 1 22:38:11 2008 Subject: [Histonet] problem with vibratome slicing Message-ID: <20080301233748.3cqfxx2fk80k0k4k@webmail.utoronto.ca> Hi My last e-mail did not appeared in the list, for I do not wat reason. Well, I was telling you that I am trying to have nice sections mounted right away in vibratome, in poly-l-lysine coated slides. Everyting is fine, until I want to wash the brain sections with water to perform and HE counterstaining, and the sections fall off. What could be happen? why with gelatin coated slides this does not happen? I have to do it with p-l-l because my protocol requires HAR and gelatin does not support that. Thank you very much Alonso From rjbuesa <@t> yahoo.com Sun Mar 2 10:27:41 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Mar 2 10:27:46 2008 Subject: [Histonet] problem with vibratome slicing In-Reply-To: <20080301233748.3cqfxx2fk80k0k4k@webmail.utoronto.ca> Message-ID: <592816.73292.qm@web65715.mail.ac4.yahoo.com> If you are washing with distilled water, that can be the cause. Also after the sections are in the slide fix them in NBF for at least 5 minutes. You could fix them with NBF vapors: place 5 mL of NBF in a Coplin jar, add the slides (the sections will not be touching the NBF), cap the Coplin jar and place it in a water bath at 60?C for 5 minutes (after the contents reached the water bath temperature). Take the slides out and wash them gently in tap water. I think they will survive if you procede tis way. Ren? J. alonso.martinezcanabal@utoronto.ca wrote: Hi My last e-mail did not appeared in the list, for I do not wat reason. Well, I was telling you that I am trying to have nice sections mounted right away in vibratome, in poly-l-lysine coated slides. Everyting is fine, until I want to wash the brain sections with water to perform and HE counterstaining, and the sections fall off. What could be happen? why with gelatin coated slides this does not happen? I have to do it with p-l-l because my protocol requires HAR and gelatin does not support that. Thank you very much Alonso _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From bdornier <@t> gmail.com Sun Mar 2 11:51:18 2008 From: bdornier <@t> gmail.com (Brandon Dornier) Date: Sun Mar 2 11:51:28 2008 Subject: [Histonet] Forum Message-ID: <8556a07e0803020951n63effd26l71460fd4b132bad@mail.gmail.com> I would like to be removed from the mailing list. Thank You From Shirley_PHUA <@t> hsa.gov.sg Sun Mar 2 14:01:34 2008 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Sun Mar 2 14:01:53 2008 Subject: [Histonet] Shirley Phua is out-of-office ... Message-ID: I will be out of the office from 03-03-2008 to 03-03-2008. I'll be overseas 03 March 2008. Pathologists: I will process your requests when I return. If urgent, please forward your email to Henry_Kyaw@hsa.gov.sg From jqb7 <@t> CDC.GOV Mon Mar 3 05:09:24 2008 From: jqb7 <@t> CDC.GOV (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Mon Mar 3 05:09:40 2008 Subject: [Histonet] Artisan Message-ID: <34BB307EFC9A65429BBB49E330675F72045E263D@LTA3VS003.ees.hhs.gov> Hello everyone, For those of you with an Artisan special stains instrument: do you use any recycled alcohol on the instrument? Thanks, Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov From godsgalnow <@t> aol.com Mon Mar 3 08:16:04 2008 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Mon Mar 3 08:16:21 2008 Subject: [Histonet] CAP Surveys Message-ID: <8CA4B503FEE9F09-654-2A55@MBLK-M07.sysops.aol.com> Ok,? I do not want do sound like a doofus, but this is the first time that we have signed up for the MK (IHC) survey from CAP.? It came with a bunch of slides and a list to check off the Antibodies that you used, etc.? My question is (as dumb as this may sound), we have to stain one of them for an H&E first, right? Go ahead and say it, I can take it...I won't remember it anyway, I am all doped up on TheraFlu. Roxanne From barbara.verstraeten <@t> ugent.be Mon Mar 3 09:19:51 2008 From: barbara.verstraeten <@t> ugent.be (Barbara Verstraeten) Date: Mon Mar 3 09:20:04 2008 Subject: [Histonet] Immunostaining on technovit 9100 zebrafish sections Message-ID: <20080303161951.wnw56zj2o8g8ssw4@webmail.ugent.be> Dear all, I would like to do an immunostaining on zebrafish section whom I've imbedded in technovit 9100. I will work with an E-cadherin antibody and detect with fuchsine. Can somebody supply me a working protocol? Or does everbody do whole mount stainings? Thanks alot for your help! Kind regards, Barbara Verstraeten VMDB University of Ghent, Belgium From rmweber113 <@t> comcast.net Mon Mar 3 09:46:51 2008 From: rmweber113 <@t> comcast.net (rmweber113@comcast.net) Date: Mon Mar 3 09:46:59 2008 Subject: [Histonet] NY histology position Message-ID: <030320081546.12700.47CC1D6B000BCF080000319C2215578674CCCECE9D0A0D0A99039D@comcast.net> I have a position for a certified, NY state licensed Histologist available at an established GI group located in New City, NY, Rockland County. Candidate should have the knowledge to set up and maintain a state of the art facility. Knowledge of grossing, processing and routine histology of gastric biopsies required. $35.00+/hr plus benefits. Qualified candidates can email me at rmweber113@comcast.net or call me at 732 814-1170. $500.00 for a successful referral committed for 90 days. Marilynn Weber H.T.(ASCP)QIHC Twincrest From rshooki_99 <@t> yahoo.com Mon Mar 3 10:27:23 2008 From: rshooki_99 <@t> yahoo.com (richard shook) Date: Mon Mar 3 10:27:26 2008 Subject: [Histonet] herpes zoster control Message-ID: <879796.57368.qm@web36113.mail.mud.yahoo.com> I work in a derm lab and we currently added Varicella Zoster virus (VZV) to our antibodies we have some internal controls but are limited, i am currently looking for a company that may sell controls.I have found some But know one with control for paraffin embedded tissue. If anyone has any information that may help could you pass it along thank you Rich Rabkin Derm Path ____________________________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ From rjbuesa <@t> yahoo.com Mon Mar 3 10:52:07 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 3 10:52:17 2008 Subject: [Histonet] CAP Surveys In-Reply-To: <8CA4B503FEE9F09-654-2A55@MBLK-M07.sysops.aol.com> Message-ID: <934282.65927.qm@web65702.mail.ac4.yahoo.com> Dear Roxanne: Yes, one of the slides will be for an H&E. I was subscribed to this program for years and it is a very good one. Note that sometimes not all the blank slides come from the same block, so what we did was, after looking at the H&E and have an idea what we were dealing with, the best slides were used with the most "relevant" Abs for the type of lesion. Also one that was not that good was used for negative (just one/case, not per Ab). Take a deep breath and plan it carefully, the analysis you are going to receive (that will include the "generally accepted" diagnosis) will be very enlightening. Good luck! Ren? J. godsgalnow@aol.com wrote: Ok,? I do not want do sound like a doofus, but this is the first time that we have signed up for the MK (IHC) survey from CAP.? It came with a bunch of slides and a list to check off the Antibodies that you used, etc.? My question is (as dumb as this may sound), we have to stain one of them for an H&E first, right? Go ahead and say it, I can take it...I won't remember it anyway, I am all doped up on TheraFlu. Roxanne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From emerald_lake77 <@t> yahoo.com Mon Mar 3 11:10:03 2008 From: emerald_lake77 <@t> yahoo.com (GT Hebert) Date: Mon Mar 3 11:10:16 2008 Subject: [Histonet] Qdots - fading now ... not as described on spec sheets Message-ID: <899538.27162.qm@web31702.mail.mud.yahoo.com> Hello all, I have been using secondary antibodies conjugated to Qdots for more than a year now. With high success in staining, I had started switching my immunofluorescence protocols from Alexa conjugated secondaries to Qdot conjugated secondaries. Up until recently everything worked well ... and all my slides (4%PF or 10%NBF animal tissue, processed routinely - paraffin embedded) were remaining fluorescent. A few months passed by (as worked and studies shifted to other priorities) and this past February I picked up using Qdots again. With all new reagents (Qdot secondaries, buffer, etc), but the same protocol - I ran my immunos. However, in every case, my fluorescence was complete quenched (gone) the following day. Is anyone who also uses Qdots experiencing this lately? I have spoken with Invitrogen (Molecular Probes) and they have described this as a fairly recent problem and their R&D group is looking into it. However, their marketing team continues to push Qdots as brighter and longer lasting than conventional organic dyes and Alexa dyes. Any information would be appreciated. Thank you. Gustave Hebert Scientist II Wyeth Research Cambridge, MA --------------------------------- Never miss a thing. Make Yahoo your homepage. From rmweber113 <@t> comcast.net Mon Mar 3 11:57:33 2008 From: rmweber113 <@t> comcast.net (rmweber113@comcast.net) Date: Mon Mar 3 11:57:41 2008 Subject: [Histonet] Histology position in NJ Message-ID: <030320081757.27599.47CC3C0D000032FF00006BCF2215568884CCCECE9D0A0D0A99039D@comcast.net> I have a position for a Histologist available at an established GI group located in Hillsborough NJ. This is a part time position with flexible hours. Candidate should have the knowledge to set up and maintain a state of the art facility. Knowledge of grossing, processing and routine histology of gastric biopsies required. $35.00/hr. Qualified candidates can email me at rmweber113@comcast.net to call me at 732 814-1170. $500.00 for a successful referral committed and client approved for at least 90 days. Marilynn Weber H.T(ASCP)QIHC Twincrest From akemiat3377 <@t> yahoo.com Mon Mar 3 13:12:06 2008 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Mon Mar 3 13:12:16 2008 Subject: [Histonet] Pathology Client Service Position at PhenoPath Message-ID: <239964.85078.qm@web31303.mail.mud.yahoo.com> PhenoPath Laboratories in Seattle, WA has a position available. If you know of anyone that might be interested, PhenoPath Laboratories is a wonderful place to work. Please check out our website to apply. www.phenopath.com PATHOLOGY CLIENT SERVICES REPRESENTATIVE (III) Job Summary: Receive and accession pathology specimens into PhenoPath database, and assist pathologists with generation of pathology reports. Provide service and assistance to PhenoPath clients. Position Requirements: o Two to three years? experience in a pathology setting preferred. Transcription experience required; medical transcription background preferred. Detail-oriented, with proficiency in medical terminology, spelling, grammar, and punctuation, along with data entry proficiency are required. Expertise in appropriate technology, in particular transcription equipment, database entry, and Microsoft Office (notably Word and Excel). o Possess strong inter-personal skills, including the ability to work well with all levels of both internal and external client staff. Highly professional, self-motivated, proactive, and personable, with a demonstrated ability to resolve problems independently and effectively. o Demonstrate adaptability in workflow processes, and ability to multi-task and prioritize. v Must be able to type 80 Plus WPM (Note: transcription and skills assessment will be given) Overall Responsibilities may include: o Specimen Accessioning: ? Identify, troubleshoot and resolve issues, such as missing, discrepant or unclear information, and ensure that problems are addressed in a timely fashion ? Complete order forms, select Test Score Sheets and perform database entry ? Pull patient history/previous materials as appropriate and organize paperwork and distribute o Participate in generating pathology reports which may include: ? Data entry of test results and images; final formatting of reports to pathologists? specifications ? Daily reconciliation of pending cases list o Transcription Support: ? Assist with daily transcription, and provide back-up transcription support as requested ? Assist with monitoring and troubleshooting the dictation system o Perform Quality Assurance Review of Reports and maintain written documentation o Organize Cases for Send-Out for additional testing and consultation, to include: ? Prepare specimen paperwork for shipping and perform documentation process ? Coordinate block and/or slide retrieval with PhenoPath shipping representative o Respond to Internal and External Pathology Client Requests: ? Assist clients with questions and issues, and troubleshoot problems in a timely fashion. Receive and respond to inquiries, or forward to appropriate person for response. ? Ensure that specimen collection and transportation requirements are met for clients, and for PhenoPath staff, and resolve specimen transport delivery services/issues in a timely fashion. ? Provide support services for PhenoPath pathologists as requested ? Assist Client Services Manager in gathering information for CAP, NY State or other inspections by regulatory agencies, and perform administrative functions such as database retrieval and documentation of specimen TAT (turnaround time) Akemi Allison-Tacha, BS, HT(ASCP)HTL Client Services Manager PhenoPath laboratories 551 North 34th Street, Suite 100 Seattle, WA 98103-8675 Work: (206) 374-9000 Cell: (425) 941-4287 E-Mail: akemiat3377@yahoo.com From mcauliff <@t> umdnj.edu Mon Mar 3 13:20:34 2008 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Mon Mar 3 13:20:28 2008 Subject: [Histonet] Formalin (from Paraformaldehyde) stability In-Reply-To: References: Message-ID: <47CC4F82.4060108@umdnj.edu> Hi Teri: Formalin made from paraformaldehyde is stable for some time, at least a few days if not longer. Someone has published on this but I don't remember who. I used to make my fix fresh each day, it just is not necessary. Geoff Johnson, Teri wrote: > I had a question today from a researcher asking about the stability of a > formalin solution made from Paraformaldehyde. She indicated that on one > of her samples, freshly prepared fixative worked well, while one day old > fixative (same batch) did not fix properly. She assures me all > pre-fixative steps were the same. The samples were fixed for 2 hours. I > have no details about her experiments or what she was testing (I suspect > mRNA targets). > > I realize that without the addition of methanol, the solution is not as > stable would would re-polymerize with time. I'm wondering if the > fixative started re-polymerizing and that made the difference, but one > day? Is it possible for one day to make the difference between a > positive and negative result? > > How stable/instable is methylene glycol? > > I'm also thinking that by adding methanol (or using purchased 100% > formalin (37% formaldehyde)) would be useful for mRNA targets, since > aldehydes tend to fix nucleic acids poorly. > > Why do all my most difficult questions arise on Friday afternoon? > > Thanks for any insight you can give me. > > > Teri Johnson, HT(ASCP)QIHC > Managing Director Histology Facility > Stowers Institute for Medical Research > 1000 E. 50th St. > Kansas City, MO 64110 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From TJJ <@t> Stowers-Institute.org Mon Mar 3 13:33:18 2008 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Mon Mar 3 13:33:45 2008 Subject: [Histonet] Washing out formalin fixation Message-ID: Last week, a researcher here asked me what the chemical mechanism was of washing out the effects of formalin fixation on the tissues with running water. In other words, how does it work? Anybody here know? Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From annette_hall <@t> pa-ucl.com Mon Mar 3 14:01:37 2008 From: annette_hall <@t> pa-ucl.com (Annette Hall) Date: Mon Mar 3 13:53:59 2008 Subject: [Histonet] Pathway Her2neu on Benchmark Message-ID: <71320B4EBC7C15419563EAFBFCD924651E9FC210B0@hades.pa-ucl.com> For those of you who perform Her2 stains on the Ventana Benchmark: What did you do for an evaluation of the 4B5 clone compared to the 6B11? Or did you evaluate 4B5 directly against FISH? We've managed to stretch out 6B11 this far, but now we are forced into converting. Thanks for your help with this switchover. Annette J Hall, MT United Clinical Labs Micro/Histo/Cyto Supervisor Dubuque, IA 52001 NOTICE: This email may contain legally privileged information. The inform ation is for the use of only the intended recipient(s) even if addressed inc orrectly. If you are not the intended recipient, please notify the sender th at you have received it in error and then delete it along with any attachmen ts. Thank you. From JimR0712 <@t> comcast.net Mon Mar 3 14:36:17 2008 From: JimR0712 <@t> comcast.net (JimR0712@comcast.net) Date: Mon Mar 3 14:36:23 2008 Subject: [Histonet] Floor mats in Histology Lab Message-ID: <030320082036.2175.47CC614100040F5E0000087F2216566276CDCEC9CFAD0307B6@comcast.net> Any sources for floor mats for the Histology Lab ? Need one that is 18' x 4' and another that is 4' x 56'. Thought I had ordered them from Lab Safety (about 5 years ago) but cannot find them now. Thanks. From MadaryJ <@t> MedImmune.com Mon Mar 3 15:42:54 2008 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Mon Mar 3 15:44:04 2008 Subject: [Histonet] Tape versus glass again! I need answers on a new one VENDORS WELCOME Message-ID: Okay Histo land, I used tape for 6 years and loved it and did not have any of these problems folks speak of with fading or getting the old ones off. It was easy, cheap and very fast. The machine broke once in 10 years and it was a small problem. I also used glass coverslippers (three different) and I had all sorts of issues with them but loved the final product. Also a final product of glass is the best of course and my place would prefer it. Are there any glass out there that are not overpriced that just work well? Anyone have a tape they are willing to part with cheap that they do not use? Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Laboratory One Medimmune Way Gaithersburg, MD 20878 ph 301.398.4745/6360 fx 301.398.9745 To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From gdeville <@t> deltapathology.com Mon Mar 3 16:09:34 2008 From: gdeville <@t> deltapathology.com (Gwen Deville) Date: Mon Mar 3 16:09:50 2008 Subject: [Histonet] IHC antibody validation Message-ID: <000601c87d7b$447d2240$cd7766c0$@com> We would like to revise our current validation method for IHC antibodies. Recently, we've had a lot discussion and no agreement on "how to" and "to what extent" you need to validate antibodies which have already undergone manufacturer's testing for specificity and sensitivity. Should this be treated in the same manner test are validated in the clinical laboratory? Testing extensive specimens and slides? Or what is considered enough of validation for patient testing. I would appreciate it if anyone has a policy/procedure that they would be willing to share. We would like to ensure that we are in compliance with the antibody validation process. G.Deville, Histology Supv. Delta Pathology, Mid-Louisiana Box 30113, 211 Fourth Street Alexandria, LA 71301 Work: (318)473-3943 / (318) 473-3180 Mobile: (318) 729-4203 Pager: (318) 427-5444 Email: gdeville@deltapathology.com From rjbuesa <@t> yahoo.com Mon Mar 3 16:16:02 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 3 16:16:06 2008 Subject: [Histonet] Washing out formalin fixation In-Reply-To: Message-ID: <205699.59907.qm@web65703.mail.ac4.yahoo.com> You cannot "unfix" a formalin fixed tissue with running water. It does not work that way. Ren? J. "Johnson, Teri" wrote: Last week, a researcher here asked me what the chemical mechanism was of washing out the effects of formalin fixation on the tissues with running water. In other words, how does it work? Anybody here know? Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From rjbuesa <@t> yahoo.com Mon Mar 3 16:17:43 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 3 16:17:47 2008 Subject: [Histonet] Floor mats in Histology Lab In-Reply-To: <030320082036.2175.47CC614100040F5E0000087F2216566276CDCEC9CFAD0307B6@comcast.net> Message-ID: <267374.67636.qm@web65710.mail.ac4.yahoo.com> We used a company that took them away and cleaned them weekly, while leaving cleaned ones. For sure in your are there is a company that provides that service. It is not economic to buy them. Ren? J. JimR0712@comcast.net wrote: Any sources for floor mats for the Histology Lab ? Need one that is 18' x 4' and another that is 4' x 56'. Thought I had ordered them from Lab Safety (about 5 years ago) but cannot find them now. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From rjbuesa <@t> yahoo.com Mon Mar 3 16:21:03 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 3 16:21:07 2008 Subject: [Histonet] IHC antibody validation In-Reply-To: <000601c87d7b$447d2240$cd7766c0$@com> Message-ID: <580581.29806.qm@web65716.mail.ac4.yahoo.com> Gwen: You are not validating the manufacturer's antibody, you are validating YOUR processing procedure, meaning that if you do something wrong or not completely adequate, you could end with results below expectations. If you use positive tissues and use the procedure at the manufacturer's dilution rates you should get adequate results, and if you don't, something you are doing to the tissues should be reviewed/corrected. Ren? J. Gwen Deville wrote: We would like to revise our current validation method for IHC antibodies. Recently, we've had a lot discussion and no agreement on "how to" and "to what extent" you need to validate antibodies which have already undergone manufacturer's testing for specificity and sensitivity. Should this be treated in the same manner test are validated in the clinical laboratory? Testing extensive specimens and slides? Or what is considered enough of validation for patient testing. I would appreciate it if anyone has a policy/procedure that they would be willing to share. We would like to ensure that we are in compliance with the antibody validation process. G.Deville, Histology Supv. Delta Pathology, Mid-Louisiana Box 30113, 211 Fourth Street Alexandria, LA 71301 Work: (318)473-3943 / (318) 473-3180 Mobile: (318) 729-4203 Pager: (318) 427-5444 Email: gdeville@deltapathology.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From Barry.R.Rittman <@t> uth.tmc.edu Mon Mar 3 16:25:47 2008 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Mon Mar 3 16:25:52 2008 Subject: [Histonet] Washing out formalin fixation In-Reply-To: <205699.59907.qm@web65703.mail.ac4.yahoo.com> References: <205699.59907.qm@web65703.mail.ac4.yahoo.com> Message-ID: Rene I disagree. Depends on what you call fixation. If tissue is fixed for a short period of time (say 1-2 hours) then the bonds that are formed are not permanent and can be broken by washing in running tap water. If however you fix for several hours the this becomes much more difficult due to extensive cross linking. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, March 03, 2008 4:16 PM To: Johnson, Teri; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Washing out formalin fixation You cannot "unfix" a formalin fixed tissue with running water. It does not work that way. Ren? J. "Johnson, Teri" wrote: Last week, a researcher here asked me what the chemical mechanism was of washing out the effects of formalin fixation on the tissues with running water. In other words, how does it work? Anybody here know? Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Mon Mar 3 16:35:40 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Mon Mar 3 16:35:45 2008 Subject: [Histonet] Washing out formalin fixation In-Reply-To: <205699.59907.qm@web65703.mail.ac4.yahoo.com> References: <205699.59907.qm@web65703.mail.ac4.yahoo.com> Message-ID: <5b6eb13e0803031435y42694d8emc32bfd69765f43ea@mail.gmail.com> I think he's talking about using tap water or DI water as antigen retrieval. If you took a slide from the oven and ran it down to water and kept rinsing the slide in running tap water for a few hours, you might get some antigen retrieval out of the process when the running water removes some of the hydrogen bonds from the formaldehyde fixation. The longer the tissue has been in formalin, the more cross-linked proteins you have, and the less likely it'd work as antigen retrieval. I just don't think this works very well, at least not when I've tried it. If he wants to try antigen retrieval with just the retrieval solution (maybe overnight?) it might work. Cutting out the heat and pressure makes it take a lot longer and it would definately be ineffective w/ many antibodies. I'd tell the him it washes out the hydrogen bonds from the formaldehyde and reverses the initial addition part of the fixation process...when the proteins start getting cross-linked it won't help you much though. On Mon, Mar 3, 2008 at 2:16 PM, Rene J Buesa wrote: > You cannot "unfix" a formalin fixed tissue with running water. It does not > work that way. > Ren? J. > > "Johnson, Teri" wrote: > Last week, a researcher here asked me what the chemical mechanism was of > washing out the effects of formalin fixation on the tissues with running > water. In other words, how does it work? Anybody here know? > > Teri Johnson, HT(ASCP)QIHC > Managing Director Histology Facility > Stowers Institute for Medical Research > 1000 E. 50th St. > Kansas City, MO 64110 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Looking for last minute shopping deals? Find them fast with Yahoo! > Search. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Reuel.Cornelia <@t> tsrh.org Mon Mar 3 16:42:42 2008 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Mon Mar 3 16:42:55 2008 Subject: [Histonet] Platelets cytospin preparation Message-ID: <47CC2A82020000C50002DB58@nwcl02.tsrh.org> I am doing an IF double staining on platelet markers, CD42c, PF4, CD61 and PSTPIP1 on a cytospin prepared slides. I need help on the cytospin preparation on a clotted blood buffy coat not on a anticoagulated blood. If you can share me a protocol that you have been using will be appreciated. Thank you very much. Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 ******************************************************************************************************************* Texas Scottish Rite Hospital for Children is one of the nation's leading pediatric centers for the treatment of orthopedic conditions, certain related neurological disorders and learning disorders, such as dyslexia. This email transmission and/or its attachments may contain confidential health information, intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing it to any other party unless required to do so by law and is required to delete/destroy the information after its stated need has been fulfilled. If you are not the intended recipient, any disclosure, copying, distribution or action taken in reliance on the contents of this email transmission is prohibited. If you have received this information in error, please notify the sender immediately and delete this information. We appreciate your efforts to protect the children's confidential information. ******************************************************************************************************************* From jnocito <@t> satx.rr.com Mon Mar 3 17:39:48 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Mar 3 17:39:54 2008 Subject: [Histonet] CAP Surveys References: <8CA4B503FEE9F09-654-2A55@MBLK-M07.sysops.aol.com> Message-ID: <000a01c87d87$df098860$0302a8c0@yourxhtr8hvc4p> yep. We always did. If we needed the slide for an Immuno, we just did the immuno over the H&E. JTT ----- Original Message ----- From: To: Sent: Monday, March 03, 2008 8:16 AM Subject: [Histonet] CAP Surveys > Ok,? I do not want do sound like a doofus, but this is the first time that > we have signed up for the MK (IHC) survey from CAP.? It came with a bunch > of slides and a list to check off the Antibodies that you used, etc.? My > question is (as dumb as this may sound), we have to stain one of them for > an H&E first, right? > > > Go ahead and say it, I can take it...I won't remember it anyway, I am all > doped up on TheraFlu. > > Roxanne > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Mar 4 07:35:10 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 4 07:35:19 2008 Subject: [Histonet] Washing out formalin fixation In-Reply-To: Message-ID: <796193.61531.qm@web65702.mail.ac4.yahoo.com> Barry: You should not disagree BEFORE knowing what I was referring to. You should at least given me the benefit of the doubt and think that I was referring to a tissue COMPLETELY fixed. Disagreeing for the sake of disagreeing is unhealthy to your state of mind. Ren? J. "Rittman, Barry R" wrote: Rene I disagree. Depends on what you call fixation. If tissue is fixed for a short period of time (say 1-2 hours) then the bonds that are formed are not permanent and can be broken by washing in running tap water. If however you fix for several hours the this becomes much more difficult due to extensive cross linking. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, March 03, 2008 4:16 PM To: Johnson, Teri; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Washing out formalin fixation You cannot "unfix" a formalin fixed tissue with running water. It does not work that way. Ren? J. "Johnson, Teri" wrote: Last week, a researcher here asked me what the chemical mechanism was of washing out the effects of formalin fixation on the tissues with running water. In other words, how does it work? Anybody here know? Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From rjbuesa <@t> yahoo.com Tue Mar 4 07:45:56 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 4 07:46:03 2008 Subject: [Histonet] Washing out formalin fixation In-Reply-To: <5b6eb13e0803031435y42694d8emc32bfd69765f43ea@mail.gmail.com> Message-ID: <812814.42444.qm@web65708.mail.ac4.yahoo.com> Mark: I think you are right in assuming that Teri was referring to some sort of antigen retrieval but, if that was the case, it was a quite obscure way of asking and that is why I thought that she was referring to "washing out the formalin" because many researchers hate the smell of formaline and perhaps wanted to have a less "odorous" specimen to handle. I think that perhaps some antigen retrieval could be obtained by wahing the sections but perhaps the risk of losing the section (peel off) is very big this way. Ren? J. Mark Tarango wrote: --------------------------------- Never miss a thing. Make Yahoo your homepage. From doug <@t> ppspath.com Tue Mar 4 07:52:43 2008 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Mar 4 07:53:48 2008 Subject: SPAM-LOW: RE: [Histonet] Washing out formalin fixation In-Reply-To: <796193.61531.qm@web65702.mail.ac4.yahoo.com> Message-ID: Happy Friday!! Ohh wait, it is only Tuesday. :( Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, March 04, 2008 8:35 AM To: Rittman, Barry R; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: RE: [Histonet] Washing out formalin fixation Barry: You should not disagree BEFORE knowing what I was referring to. You should at least given me the benefit of the doubt and think that I was referring to a tissue COMPLETELY fixed. Disagreeing for the sake of disagreeing is unhealthy to your state of mind. Ren? J. "Rittman, Barry R" wrote: Rene I disagree. Depends on what you call fixation. If tissue is fixed for a short period of time (say 1-2 hours) then the bonds that are formed are not permanent and can be broken by washing in running tap water. If however you fix for several hours the this becomes much more difficult due to extensive cross linking. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, March 03, 2008 4:16 PM To: Johnson, Teri; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Washing out formalin fixation You cannot "unfix" a formalin fixed tissue with running water. It does not work that way. Ren? J. "Johnson, Teri" wrote: Last week, a researcher here asked me what the chemical mechanism was of washing out the effects of formalin fixation on the tissues with running water. In other words, how does it work? Anybody here know? Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> Stowers-Institute.org Tue Mar 4 08:32:40 2008 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Tue Mar 4 08:37:26 2008 Subject: [Histonet] Washing out formalin fixation In-Reply-To: <812814.42444.qm@web65708.mail.ac4.yahoo.com> References: <5b6eb13e0803031435y42694d8emc32bfd69765f43ea@mail.gmail.com>, <812814.42444.qm@web65708.mail.ac4.yahoo.com> Message-ID: Regardless of the application, I have been taught that formalin fixation can be washed out if you take fixed tissues and store them in water or an aqueous solution, or wash them in running water (might take days to accomplish). The question was, and still is, what is the chemical mechanism for this. Mark gives a plausible explanation. From: Rene J Buesa [rjbuesa@yahoo.com] Sent: Tuesday, March 04, 2008 7:45 AM To: Mark Tarango Cc: Johnson, Teri; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Washing out formalin fixation Mark: I think you are right in assuming that Teri was referring to some sort of antigen retrieval but, if that was the case, it was a quite obscure way of asking and that is why I thought that she was referring to "washing out the formalin" because many researchers hate the smell of formaline and perhaps wanted to have a less "odorous" specimen to handle. I think that perhaps some antigen retrieval could be obtained by wahing the sections but perhaps the risk of losing the section (peel off) is very big this way. Ren? J. Mark Tarango wrote: ________________________________ Never miss a thing. Make Yahoo your homepage. From cormier <@t> MIT.EDU Tue Mar 4 09:07:58 2008 From: cormier <@t> MIT.EDU (Kathy Cormier) Date: Tue Mar 4 09:08:11 2008 Subject: [Histonet] Coombs Tests Message-ID: <000601c87e09$8896aa80$92003712@mit.edu> I was looking to run a Coombs Test for a Primate sample, any suggestions on who is running this? Any suggestions would be appreciated. Thanks. From rmweber113 <@t> comcast.net Tue Mar 4 09:39:25 2008 From: rmweber113 <@t> comcast.net (rmweber113@comcast.net) Date: Tue Mar 4 09:39:33 2008 Subject: FW: [Histonet] Histology position in NJ Message-ID: <030420081539.21376.47CD6D2C000F3C52000053802216527966CCCECE9D0A0D0A99039D@comcast.net> -------------- Forwarded Message: -------------- From: rmweber113@comcast.net To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology position in NJ Date: Mon, 3 Mar 2008 17:59:00 +0000 I have a position for a Histologist available at an established GI group located in Hillsborough NJ. This is a part time position with flexible hours. Candidate should have the knowledge to set up and maintain a state of the art facility. Knowledge of grossing, processing and routine histology of gastric biopsies required. $35.00/hr. Qualified candidates can email me at rmweber113@comcast.net to call me at 732 814-1170. $500.00 for a successful referral committed and client approved for at least 90 days. Marilynn Weber H.T(ASCP)QIHC Twincrest _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bhewlett <@t> cogeco.ca Tue Mar 4 09:56:18 2008 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Tue Mar 4 09:56:27 2008 Subject: [Histonet] Washing out formalin fixation (Lengthy) References: Message-ID: <000d01c87e10$49506080$6500a8c0@mainbox> Hi Teri and everyone else on this thread, Washing out many of the effects of formaldehyde fixation on tissues with running water has been known for years (much longer than this old boy has been around). In modern terms, it is the essential underlying mechanism for so-called antigen retrieval (HIER). Formaldehyde fixes proteins by addition, with the formation of hydroxymethyl adducts on the reactive side chains of proteins. Once enough of these hydroxymethyl adducts are formed, and IF they are in close approximation to each other, they may slowly cross-link by the formation of methylene bridges. However, these adducts and initial cross-links are unstable and readily reversed by water and alcohol (see Kiernan (1999). It takes 24 hours at room temperature for all the hydroxymethyl adducts to form, i.e. maximal binding threshold (see Fox et al, 1985). If the tissue is then exposed to running water before all the adducts have formed (i.e. less than 24 hours), the reversal is very rapid. The shorter the time in formaldehyde, the more rapid the reversal. Even after the essential 24 hours fixation and also after a more lengthy 6 days fixation, running water will still remove the adducts and hydrolyse the methylene bridges. There is at least one publication (Helander, 1994) that provides data regarding this effect. After 24 hours fixation, 50% reversal occurred in less than 24 hours, 90% reversal was obtained after 6 days washing and for 6 days fixation 90% reversal after 4 weeks washing. It should be noted that these reversal times were obtained at ambient temperatures and the times may be considerably reduced by elevated temperatures. This reversal effect is also obtained on tissue sections that have been processed to wax. However, because of the additional shrinkage and hydrophobicity of the processed proteins, the reversal is slowed somewhat until the proteins re-hydrate. The reversal effect can also be aided by the presence of other ions in the water (the purpose of HIER buffers). Back in the sixties, in order to successfully demonstrate Ig's by IF, we were reversing the fixation effects on paraffin sections by placing them in hypotonic buffers for 2 days at 37C. Today, since we are all in a great rush for results, we obviously drive the reversal at higher temperatures to speed things up!! References: Baker JR (1958), Principles of Biological Microtechnique, Methuen & Co. Ltd. Hopwood D. Fixatives and fixation: A review. Histochemical journal (1969); 1, p19-55 Burnett MG. The mechanism of the formaldehyde clock reaction: Methylene glycol dehydration. J Chem. educ. (1982); 59, 160 Fox CH et al. Formaldehyde fixation. J Histochem Cytochem (1985); 33, 845-853 Helander KG. Kinetic studies of formaldehyde binding in tissue. Biotechnique and Histochemistry. (1994); 69, 177-179 Kiernan J.A., Histological and Histochemical Methods: Theory and Practice, 3rd Edition, (1999). Oxford: Butterworth-Heinemann. ISBN # 0-7506-3106-6. Shi SR, Gu J. and Taylor CR. Antigen Retrieval Techniques: Immunohistochemistry and Molecular Morphology. (2000) Eaton Publishing. ISBN 1-881299-43-0. Sompuram SR, Vani K, Messana E and Bogen SA. A molecular mechanism of formalin fixation and antigen retrieval. Am J Clin Pathol (2004);121: 190-199 Best regards, Bryan ----- Original Message ----- From: "Johnson, Teri" To: Sent: Monday, March 03, 2008 2:33 PM Subject: [Histonet] Washing out formalin fixation Last week, a researcher here asked me what the chemical mechanism was of washing out the effects of formalin fixation on the tissues with running water. In other words, how does it work? Anybody here know? Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Tue Mar 4 10:04:30 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Mar 4 10:04:42 2008 Subject: AW: [Histonet] Washing out formalin fixation In-Reply-To: Message-ID: <000001c87e11$6f060b30$eeeea8c0@dielangs.at> Formaldehyde is bound to makromolecules as methylol-groups or Schiff-bases in the first step. These formations are said to be unstable and can be removed. The crosslinking is a second step, where stable methylenbridges are built. These should be stable and withstand the washing. The linkages between formaldehyd and various tissue-compounds have different power, depending on the reactionpartners and the milieu. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Johnson, Teri Gesendet: Montag, 03. M?rz 2008 20:33 An: 'histonet@lists.utsouthwestern.edu' Betreff: [Histonet] Washing out formalin fixation Last week, a researcher here asked me what the chemical mechanism was of washing out the effects of formalin fixation on the tissues with running water. In other words, how does it work? Anybody here know? Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Tue Mar 4 11:09:23 2008 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Mar 4 11:09:27 2008 Subject: [Histonet] Coombs Tests In-Reply-To: <000601c87e09$8896aa80$92003712@mit.edu> Message-ID: Any blood bank laboratory will run a Coombs test. Try a local hospital. If you have a CLS/MT program at the school they should also be able to do this also, or have the contacts to have it done. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Kathy Cormier" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/04/2008 07:07 AM To cc Subject [Histonet] Coombs Tests I was looking to run a Coombs Test for a Primate sample, any suggestions on who is running this? Any suggestions would be appreciated. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tim.morken <@t> thermofisher.com Tue Mar 4 11:16:49 2008 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Tue Mar 4 11:17:28 2008 Subject: [Histonet] Washing out formalin fixation (Lengthy) In-Reply-To: <000d01c87e10$49506080$6500a8c0@mainbox> References: <000d01c87e10$49506080$6500a8c0@mainbox> Message-ID: <6BFF6D137DF6BC43B33891BA96E83B19013A8278@PGHCR-EXMB-VS-1.na.fshrnet.com> Thanks for the synopsis Bryan. I'd like to point out that the 2004 paper by Sompuram,and a followup in 2006 are very interesting. These studies these show that fixation of peptide spots (essentially zero thickness) attached to glass slides take over 6 hours to "fix" at room temperature. In this case they defined "fixed" as the point at which an antibody would no longer detect it's target epitope, or the reaction was severly diminished. They also showed that HIER reverses the "fixation." This calls into question any method that relies on less than six hours formalin fixation at room temperature (ie, biospies, just because they are small) and also the effect of exposing those short-fixed tissues to long exposure to 70% alcohol, or other aqueous solution, before clearing and embedding. The 2006 paper also investigates why some epitopes are affected by formalin and others are not. Sompuram SR, Vani K, Messana E and Bogen SA. A molecular mechanism of formalin fixation and antigen retrieval. Am J Clin Pathol (2004);121: 190-199 Sompuram, et. al, A molecular model of antigen retrieval using a peptide array, Am J Clin Path 2006;125:91-98 Tim Morken Technical Support Manager Lab Vision Products Anatomical Pathology ThermoFisher Scientific -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan Hewlett Sent: Tuesday, March 04, 2008 7:56 AM To: Johnson, Teri; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Washing out formalin fixation (Lengthy) Hi Teri and everyone else on this thread, Washing out many of the effects of formaldehyde fixation on tissues with running water has been known for years (much longer than this old boy has been around). In modern terms, it is the essential underlying mechanism for so-called antigen retrieval (HIER). Formaldehyde fixes proteins by addition, with the formation of hydroxymethyl adducts on the reactive side chains of proteins. Once enough of these hydroxymethyl adducts are formed, and IF they are in close approximation to each other, they may slowly cross-link by the formation of methylene bridges. However, these adducts and initial cross-links are unstable and readily reversed by water and alcohol (see Kiernan (1999). It takes 24 hours at room temperature for all the hydroxymethyl adducts to form, i.e. maximal binding threshold (see Fox et al, 1985). If the tissue is then exposed to running water before all the adducts have formed (i.e. less than 24 hours), the reversal is very rapid. The shorter the time in formaldehyde, the more rapid the reversal. Even after the essential 24 hours fixation and also after a more lengthy 6 days fixation, running water will still remove the adducts and hydrolyse the methylene bridges. There is at least one publication (Helander, 1994) that provides data regarding this effect. After 24 hours fixation, 50% reversal occurred in less than 24 hours, 90% reversal was obtained after 6 days washing and for 6 days fixation 90% reversal after 4 weeks washing. It should be noted that these reversal times were obtained at ambient temperatures and the times may be considerably reduced by elevated temperatures. This reversal effect is also obtained on tissue sections that have been processed to wax. However, because of the additional shrinkage and hydrophobicity of the processed proteins, the reversal is slowed somewhat until the proteins re-hydrate. The reversal effect can also be aided by the presence of other ions in the water (the purpose of HIER buffers). Back in the sixties, in order to successfully demonstrate Ig's by IF, we were reversing the fixation effects on paraffin sections by placing them in hypotonic buffers for 2 days at 37C. Today, since we are all in a great rush for results, we obviously drive the reversal at higher temperatures to speed things up!! References: Baker JR (1958), Principles of Biological Microtechnique, Methuen & Co. Ltd. Hopwood D. Fixatives and fixation: A review. Histochemical journal (1969); 1, p19-55 Burnett MG. The mechanism of the formaldehyde clock reaction: Methylene glycol dehydration. J Chem. educ. (1982); 59, 160 Fox CH et al. Formaldehyde fixation. J Histochem Cytochem (1985); 33, 845-853 Helander KG. Kinetic studies of formaldehyde binding in tissue. Biotechnique and Histochemistry. (1994); 69, 177-179 Kiernan J.A., Histological and Histochemical Methods: Theory and Practice, 3rd Edition, (1999). Oxford: Butterworth-Heinemann. ISBN # 0-7506-3106-6. Shi SR, Gu J. and Taylor CR. Antigen Retrieval Techniques: Immunohistochemistry and Molecular Morphology. (2000) Eaton Publishing. ISBN 1-881299-43-0. Sompuram SR, Vani K, Messana E and Bogen SA. A molecular mechanism of formalin fixation and antigen retrieval. Am J Clin Pathol (2004);121: 190-199 Best regards, Bryan ----- Original Message ----- From: "Johnson, Teri" To: Sent: Monday, March 03, 2008 2:33 PM Subject: [Histonet] Washing out formalin fixation Last week, a researcher here asked me what the chemical mechanism was of washing out the effects of formalin fixation on the tissues with running water. In other words, how does it work? Anybody here know? Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Tue Mar 4 11:35:04 2008 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Tue Mar 4 11:34:41 2008 Subject: [Histonet] Washing out formalin fixation (Lengthy) Message-ID: <5C0BED61F529364E86309CADEA63FEF20163F358@TRFT-EX01.xRothGen.nhs.uk> "These studies these show that fixation of peptide spots (essentially zero thickness) attached to glass slides take over 6 hours to "fix" at room temperature. In this case they defined "fixed" as the point at which an antibody would no longer detect it's target epitope, or the reaction was severely diminished. They also showed that HIER reverses the "fixation" So far so good, and supports what I have been saying in this forum for years about the (long) time it takes to formalin fix tissue, and the misbelief that wetting them for a few hours is fixing. But then...... "This calls into question any method that relies on less than six hours formalin fixation at room temperature (ie, biopsies, just because they are small) and also the effect of exposing those short-fixed tissues to long exposure to 70% alcohol, or other aqueous solution, before clearing and embedding." I know not what this all means/is getting at. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: 04 March 2008 17:17 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Washing out formalin fixation (Lengthy) Thanks for the synopsis Bryan. I'd like to point out that the 2004 paper by Sompuram,and a followup in 2006 are very interesting. These studies these show that fixation of peptide spots (essentially zero thickness) attached to glass slides take over 6 hours to "fix" at room temperature. In this case they defined "fixed" as the point at which an antibody would no longer detect it's target epitope, or the reaction was severly diminished. They also showed that HIER reverses the "fixation." The 2006 paper also investigates why some epitopes are affected by formalin and others are not. Sompuram SR, Vani K, Messana E and Bogen SA. A molecular mechanism of formalin fixation and antigen retrieval. Am J Clin Pathol (2004);121: 190-199 Sompuram, et. al, A molecular model of antigen retrieval using a peptide array, Am J Clin Path 2006;125:91-98 Tim Morken Technical Support Manager Lab Vision Products Anatomical Pathology ThermoFisher Scientific -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan Hewlett Sent: Tuesday, March 04, 2008 7:56 AM To: Johnson, Teri; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Washing out formalin fixation (Lengthy) Hi Teri and everyone else on this thread, Washing out many of the effects of formaldehyde fixation on tissues with running water has been known for years (much longer than this old boy has been around). In modern terms, it is the essential underlying mechanism for so-called antigen retrieval (HIER). Formaldehyde fixes proteins by addition, with the formation of hydroxymethyl adducts on the reactive side chains of proteins. Once enough of these hydroxymethyl adducts are formed, and IF they are in close approximation to each other, they may slowly cross-link by the formation of methylene bridges. However, these adducts and initial cross-links are unstable and readily reversed by water and alcohol (see Kiernan (1999). It takes 24 hours at room temperature for all the hydroxymethyl adducts to form, i.e. maximal binding threshold (see Fox et al, 1985). If the tissue is then exposed to running water before all the adducts have formed (i.e. less than 24 hours), the reversal is very rapid. The shorter the time in formaldehyde, the more rapid the reversal. Even after the essential 24 hours fixation and also after a more lengthy 6 days fixation, running water will still remove the adducts and hydrolyse the methylene bridges. There is at least one publication (Helander, 1994) that provides data regarding this effect. After 24 hours fixation, 50% reversal occurred in less than 24 hours, 90% reversal was obtained after 6 days washing and for 6 days fixation 90% reversal after 4 weeks washing. It should be noted that these reversal times were obtained at ambient temperatures and the times may be considerably reduced by elevated temperatures. This reversal effect is also obtained on tissue sections that have been processed to wax. However, because of the additional shrinkage and hydrophobicity of the processed proteins, the reversal is slowed somewhat until the proteins re-hydrate. The reversal effect can also be aided by the presence of other ions in the water (the purpose of HIER buffers). Back in the sixties, in order to successfully demonstrate Ig's by IF, we were reversing the fixation effects on paraffin sections by placing them in hypotonic buffers for 2 days at 37C. Today, since we are all in a great rush for results, we obviously drive the reversal at higher temperatures to speed things up!! References: Baker JR (1958), Principles of Biological Microtechnique, Methuen & Co. Ltd. Hopwood D. Fixatives and fixation: A review. Histochemical journal (1969); 1, p19-55 Burnett MG. The mechanism of the formaldehyde clock reaction: Methylene glycol dehydration. J Chem. educ. (1982); 59, 160 Fox CH et al. Formaldehyde fixation. J Histochem Cytochem (1985); 33, 845-853 Helander KG. Kinetic studies of formaldehyde binding in tissue. Biotechnique and Histochemistry. (1994); 69, 177-179 Kiernan J.A., Histological and Histochemical Methods: Theory and Practice, 3rd Edition, (1999). Oxford: Butterworth-Heinemann. ISBN # 0-7506-3106-6. Shi SR, Gu J. and Taylor CR. Antigen Retrieval Techniques: Immunohistochemistry and Molecular Morphology. (2000) Eaton Publishing. ISBN 1-881299-43-0. Sompuram SR, Vani K, Messana E and Bogen SA. A molecular mechanism of formalin fixation and antigen retrieval. Am J Clin Pathol (2004);121: 190-199 Best regards, Bryan ----- Original Message ----- From: "Johnson, Teri" To: Sent: Monday, March 03, 2008 2:33 PM Subject: [Histonet] Washing out formalin fixation Last week, a researcher here asked me what the chemical mechanism was of washing out the effects of formalin fixation on the tissues with running water. In other words, how does it work? Anybody here know? Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ###################################################################### This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. ###################################################################### From pkarlisch <@t> hmc.psu.edu Tue Mar 4 11:43:42 2008 From: pkarlisch <@t> hmc.psu.edu (Patricia Karlisch) Date: Tue Mar 4 11:44:20 2008 Subject: [Histonet] What happened to BIOMEDA Message-ID: <47CD43FD.07B7.008C.0@hmc.psu.edu> Histonetters, I can not contact Biomeda any longer. Does anyone know what happened? Thanks, Pat Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. From making <@t> ufl.edu Tue Mar 4 11:48:16 2008 From: making <@t> ufl.edu (MKing) Date: Tue Mar 4 11:48:35 2008 Subject: [Histonet] Re: Histonet Digest, Vol 52, Issue 4 In-Reply-To: <200803041718.m24HIIEO024076@smtp.ufl.edu> References: <200803041718.m24HIIEO024076@smtp.ufl.edu> Message-ID: <47CD8B60.3030101@ufl.edu> Bryan and Tim, Thanks for excellent postsMK, this info will go in my tech folder! It looked like this thread was headed into a flame war, nice to have substantiated data prevail here. Mike King UF Pharmacology & Therapeutics -------------------- Date: Tue, 4 Mar 2008 10:56:18 -0500 From: "Bryan Hewlett" Subject: Re: [Histonet] Washing out formalin fixation (Lengthy) From bhewlett <@t> cogeco.ca Tue Mar 4 11:55:19 2008 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Tue Mar 4 11:55:28 2008 Subject: [Histonet] Washing out formalin fixation (Lengthy) References: <000d01c87e10$49506080$6500a8c0@mainbox> <6BFF6D137DF6BC43B33891BA96E83B19013A8278@PGHCR-EXMB-VS-1.na.fshrnet.com> Message-ID: <002401c87e20$e90325d0$6500a8c0@mainbox> Hi Tim, Thanks, I am aware of both papers and also a follow-up discussion at BSC last year. The authors also indicate that some epitopes do not mask or require HIER to demonstrate, even though they have been 'fixed' for long enough. This is of course born out by every day experience in the IHC lab. Interestingly, they also point out that some peptides that are fixed this way and do not require HIER, WILL require HIER if fixed in the presence of another protein or peptide. That of course is also the case in the real world of tissues!!! (See also Yang K, Sompuram SR, Fitzgibbons P Bogen SA. National HER2 proficiency test results using standardized quantitative controls: characterization of laboratory failures. Arch Pathol Lab Med 112, February 2008 pp 211-216.) As you know, I have long decried the practice of short fixing small biopsies and the effects of reversal and refixation by alcohol during processing. This can be particularly devastating on surface proteins such as HER2 etc. etc. The 6 hour minimum fixation time in the HER2 guidelines is, in my opinion, wrong!!! OK, I'll step off the soap box. cheers, Bryan ----- Original Message ----- From: "Morken, Tim" To: Sent: Tuesday, March 04, 2008 12:16 PM Subject: RE: [Histonet] Washing out formalin fixation (Lengthy) Thanks for the synopsis Bryan. I'd like to point out that the 2004 paper by Sompuram,and a followup in 2006 are very interesting. These studies these show that fixation of peptide spots (essentially zero thickness) attached to glass slides take over 6 hours to "fix" at room temperature. In this case they defined "fixed" as the point at which an antibody would no longer detect it's target epitope, or the reaction was severly diminished. They also showed that HIER reverses the "fixation." This calls into question any method that relies on less than six hours formalin fixation at room temperature (ie, biospies, just because they are small) and also the effect of exposing those short-fixed tissues to long exposure to 70% alcohol, or other aqueous solution, before clearing and embedding. The 2006 paper also investigates why some epitopes are affected by formalin and others are not. Sompuram SR, Vani K, Messana E and Bogen SA. A molecular mechanism of formalin fixation and antigen retrieval. Am J Clin Pathol (2004);121: 190-199 Sompuram, et. al, A molecular model of antigen retrieval using a peptide array, Am J Clin Path 2006;125:91-98 Tim Morken Technical Support Manager Lab Vision Products Anatomical Pathology ThermoFisher Scientific -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan Hewlett Sent: Tuesday, March 04, 2008 7:56 AM To: Johnson, Teri; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Washing out formalin fixation (Lengthy) Hi Teri and everyone else on this thread, Washing out many of the effects of formaldehyde fixation on tissues with running water has been known for years (much longer than this old boy has been around). In modern terms, it is the essential underlying mechanism for so-called antigen retrieval (HIER). Formaldehyde fixes proteins by addition, with the formation of hydroxymethyl adducts on the reactive side chains of proteins. Once enough of these hydroxymethyl adducts are formed, and IF they are in close approximation to each other, they may slowly cross-link by the formation of methylene bridges. However, these adducts and initial cross-links are unstable and readily reversed by water and alcohol (see Kiernan (1999). It takes 24 hours at room temperature for all the hydroxymethyl adducts to form, i.e. maximal binding threshold (see Fox et al, 1985). If the tissue is then exposed to running water before all the adducts have formed (i.e. less than 24 hours), the reversal is very rapid. The shorter the time in formaldehyde, the more rapid the reversal. Even after the essential 24 hours fixation and also after a more lengthy 6 days fixation, running water will still remove the adducts and hydrolyse the methylene bridges. There is at least one publication (Helander, 1994) that provides data regarding this effect. After 24 hours fixation, 50% reversal occurred in less than 24 hours, 90% reversal was obtained after 6 days washing and for 6 days fixation 90% reversal after 4 weeks washing. It should be noted that these reversal times were obtained at ambient temperatures and the times may be considerably reduced by elevated temperatures. This reversal effect is also obtained on tissue sections that have been processed to wax. However, because of the additional shrinkage and hydrophobicity of the processed proteins, the reversal is slowed somewhat until the proteins re-hydrate. The reversal effect can also be aided by the presence of other ions in the water (the purpose of HIER buffers). Back in the sixties, in order to successfully demonstrate Ig's by IF, we were reversing the fixation effects on paraffin sections by placing them in hypotonic buffers for 2 days at 37C. Today, since we are all in a great rush for results, we obviously drive the reversal at higher temperatures to speed things up!! References: Baker JR (1958), Principles of Biological Microtechnique, Methuen & Co. Ltd. Hopwood D. Fixatives and fixation: A review. Histochemical journal (1969); 1, p19-55 Burnett MG. The mechanism of the formaldehyde clock reaction: Methylene glycol dehydration. J Chem. educ. (1982); 59, 160 Fox CH et al. Formaldehyde fixation. J Histochem Cytochem (1985); 33, 845-853 Helander KG. Kinetic studies of formaldehyde binding in tissue. Biotechnique and Histochemistry. (1994); 69, 177-179 Kiernan J.A., Histological and Histochemical Methods: Theory and Practice, 3rd Edition, (1999). Oxford: Butterworth-Heinemann. ISBN # 0-7506-3106-6. Shi SR, Gu J. and Taylor CR. Antigen Retrieval Techniques: Immunohistochemistry and Molecular Morphology. (2000) Eaton Publishing. ISBN 1-881299-43-0. Sompuram SR, Vani K, Messana E and Bogen SA. A molecular mechanism of formalin fixation and antigen retrieval. Am J Clin Pathol (2004);121: 190-199 Best regards, Bryan ----- Original Message ----- From: "Johnson, Teri" To: Sent: Monday, March 03, 2008 2:33 PM Subject: [Histonet] Washing out formalin fixation Last week, a researcher here asked me what the chemical mechanism was of washing out the effects of formalin fixation on the tissues with running water. In other words, how does it work? Anybody here know? Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From burch007 <@t> mc.duke.edu Tue Mar 4 12:05:35 2008 From: burch007 <@t> mc.duke.edu (James L Burchette) Date: Tue Mar 4 12:05:45 2008 Subject: [Histonet] What happened to BIOMEDA In-Reply-To: <47CD43FD.07B7.008C.0@hmc.psu.edu> Message-ID: I heard from several vendors that Jose Perdomo (founder/president of Biomeda) passed away and the company went out of business. Jim Burchette "Patricia Karlisch" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/04/2008 12:43 PM To cc Subject [Histonet] What happened to BIOMEDA Histonetters, I can not contact Biomeda any longer. Does anyone know what happened? Thanks, Pat Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From melissa.mazan <@t> tufts.edu Tue Mar 4 12:10:04 2008 From: melissa.mazan <@t> tufts.edu (Melissa Mazan) Date: Tue Mar 4 12:10:14 2008 Subject: [Histonet] fixation times In-Reply-To: <200803041801.m24I1b5D024717@mail-proofpoint-2a.usg.tufts.edu> References: <200803041801.m24I1b5D024717@mail-proofpoint-2a.usg.tufts.edu> Message-ID: <47CD907C.1070106@tufts.edu> This thread has been very interesting - and I have a practical question. We do IHC - both immunofluorescent and immunoenzyme - on lung tissue in our lab. We generally try to fix the tissues in formalin for at least 4 hours and no more than 6 hours - as we tend to get better antibody binding - this is even with HIER. Is there an optimum amount of time that the group recommends? Thanks - Melissa histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Washing out formalin fixation (Lengthy) > (Marshall Terry Dr, Consultant Histopathologist) > 2. What happened to BIOMEDA (Patricia Karlisch) > 3. Re: Histonet Digest, Vol 52, Issue 4 (MKing) > 4. Re: Washing out formalin fixation (Lengthy) (Bryan Hewlett) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 4 Mar 2008 17:35:04 -0000 > From: "Marshall Terry Dr, Consultant Histopathologist" > > Subject: RE: [Histonet] Washing out formalin fixation (Lengthy) > To: "Morken, Tim" , > > Message-ID: > <5C0BED61F529364E86309CADEA63FEF20163F358@TRFT-EX01.xRothGen.nhs.uk> > Content-Type: text/plain; charset="us-ascii" > > "These studies these show that fixation of peptide spots (essentially > zero thickness) attached to glass slides take over 6 hours to "fix" at > room temperature. In this case they defined "fixed" as the point at > which an antibody would no longer detect it's target epitope, or the > reaction was severely diminished. They also showed that HIER reverses > the "fixation" > > So far so good, and supports what I have been saying in this forum for > years about the (long) time it takes to formalin fix tissue, and the > misbelief that wetting them for a few hours is fixing. > But then...... > > "This calls into question any method that relies on less than six hours > formalin fixation at room temperature (ie, biopsies, just because they > are small) and also the effect of exposing those short-fixed tissues to > long exposure to 70% alcohol, or other aqueous solution, before clearing > and embedding." > > I know not what this all means/is getting at. > > Terry > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, > Tim > Sent: 04 March 2008 17:17 > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Washing out formalin fixation (Lengthy) > > > Thanks for the synopsis Bryan. > > I'd like to point out that the 2004 paper by Sompuram,and a followup in > 2006 are very interesting. These studies these show that fixation of > peptide spots (essentially zero thickness) attached to glass slides take > over 6 hours to "fix" at room temperature. In this case they defined > "fixed" as the point at which an antibody would no longer detect it's > target epitope, or the reaction was severly diminished. They also showed > that HIER reverses the "fixation." > > > The 2006 paper also investigates why some epitopes are affected by > formalin and others are not. > > Sompuram SR, Vani K, Messana E and Bogen SA. A molecular mechanism of > formalin fixation and antigen retrieval. Am J Clin Pathol (2004);121: > 190-199 > > Sompuram, et. al, A molecular model of antigen retrieval using a peptide > array, Am J Clin Path 2006;125:91-98 > > > Tim Morken > Technical Support Manager > Lab Vision Products > Anatomical Pathology > ThermoFisher Scientific > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan > Hewlett > Sent: Tuesday, March 04, 2008 7:56 AM > To: Johnson, Teri; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Washing out formalin fixation (Lengthy) > > Hi Teri and everyone else on this thread, > > Washing out many of the effects of formaldehyde fixation on tissues with > running water has been known for years (much longer than this old boy > has been around). > In modern terms, it is the essential underlying mechanism for so-called > antigen retrieval (HIER). > > Formaldehyde fixes proteins by addition, with the formation of > hydroxymethyl adducts on the reactive side chains of proteins. > Once enough of these hydroxymethyl adducts are formed, and IF they are > in close approximation to each other, they may slowly cross-link by the > formation of methylene bridges. > However, these adducts and initial cross-links are unstable and readily > reversed by water and alcohol (see Kiernan (1999). > It takes 24 hours at room temperature for all the hydroxymethyl adducts > to form, i.e. maximal binding threshold (see Fox et al, 1985). > If the tissue is then exposed to running water before all the adducts > have formed (i.e. less than 24 hours), the reversal is very rapid. > The shorter the time in formaldehyde, the more rapid the reversal. > Even after the essential 24 hours fixation and also after a more lengthy > 6 days fixation, running water will still remove the adducts and > hydrolyse the methylene bridges. > There is at least one publication (Helander, 1994) that provides data > regarding this effect. > After 24 hours fixation, 50% reversal occurred in less than 24 hours, > 90% reversal was obtained after 6 days washing and for 6 days fixation > 90% reversal after 4 weeks washing. > It should be noted that these reversal times were obtained at ambient > temperatures and the times may be considerably reduced by elevated > temperatures. > > This reversal effect is also obtained on tissue sections that have been > processed to wax. > However, because of the additional shrinkage and hydrophobicity of the > processed proteins, the reversal is slowed somewhat until the proteins > re-hydrate. > The reversal effect can also be aided by the presence of other ions in > the water (the purpose of HIER buffers). > Back in the sixties, in order to successfully demonstrate Ig's by IF, we > were reversing the fixation effects on paraffin sections by placing them > in hypotonic buffers for 2 days at 37C. > Today, since we are all in a great rush for results, we obviously drive > the reversal at higher temperatures to speed things up!! > > References: > > Baker JR (1958), Principles of Biological Microtechnique, Methuen & Co. > Ltd. > Hopwood D. Fixatives and fixation: A review. Histochemical journal > (1969); 1, p19-55 Burnett MG. The mechanism of the formaldehyde clock > reaction: Methylene glycol dehydration. J Chem. educ. (1982); 59, 160 > Fox CH et al. Formaldehyde fixation. J Histochem Cytochem (1985); 33, > 845-853 > Helander KG. Kinetic studies of formaldehyde binding in tissue. > Biotechnique and Histochemistry. (1994); 69, 177-179 Kiernan J.A., > Histological and Histochemical Methods: Theory and Practice, 3rd > Edition, (1999). Oxford: Butterworth-Heinemann. ISBN # 0-7506-3106-6. > Shi SR, Gu J. and Taylor CR. Antigen Retrieval Techniques: > Immunohistochemistry and Molecular Morphology. (2000) Eaton Publishing. > ISBN 1-881299-43-0. > Sompuram SR, Vani K, Messana E and Bogen SA. A molecular mechanism of > formalin fixation and antigen retrieval. Am J Clin Pathol (2004);121: > 190-199 > > > Best regards, > > Bryan > > ----- Original Message ----- > From: "Johnson, Teri" > To: > Sent: Monday, March 03, 2008 2:33 PM > Subject: [Histonet] Washing out formalin fixation > > > Last week, a researcher here asked me what the chemical mechanism was of > > washing out the effects of formalin fixation on the tissues with running > > water. In other words, how does it work? Anybody here know? > > Teri Johnson, HT(ASCP)QIHC > Managing Director Histology Facility > Stowers Institute for Medical Research > 1000 E. 50th St. > Kansas City, MO 64110 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ###################################################################### > This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. > The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. > If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. > ###################################################################### > > > ------------------------------ > > Message: 2 > Date: Tue, 04 Mar 2008 12:43:42 -0500 > From: "Patricia Karlisch" > Subject: [Histonet] What happened to BIOMEDA > To: > Message-ID: <47CD43FD.07B7.008C.0@hmc.psu.edu> > Content-Type: text/plain; charset=US-ASCII > > Histonetters, > I can not contact Biomeda any longer. Does anyone know what happened? Thanks, Pat > > Pat Karlisch > Supervisor, Histology, Pathology and Laboratory Medicine > Penn State Milton S. Hershey Medical Center > Mail Code H179 > Hershey, PA 17033 > Phone (717) 531-6072 > Fax: (717) 531- 7741 > email: pkarlisch@psu.edu > > *****E-Mail Confidentiality Notice***** > This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. > > > ------------------------------ > > Message: 3 > Date: Tue, 04 Mar 2008 12:48:16 -0500 > From: MKing > Subject: [Histonet] Re: Histonet Digest, Vol 52, Issue 4 > To: histonet@lists.utsouthwestern.edu > Message-ID: <47CD8B60.3030101@ufl.edu> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Bryan and Tim, > Thanks for excellent postsMK, this info will go in my tech folder! It > looked like this thread was headed into a flame war, nice to have > substantiated data prevail here. > Mike King > UF Pharmacology & Therapeutics > -------------------- > Date: Tue, 4 Mar 2008 10:56:18 -0500 > From: "Bryan Hewlett" > Subject: Re: [Histonet] Washing out formalin fixation (Lengthy) > > > > > ------------------------------ > > Message: 4 > Date: Tue, 4 Mar 2008 12:55:19 -0500 > From: "Bryan Hewlett" > Subject: Re: [Histonet] Washing out formalin fixation (Lengthy) > To: , "Morken, Tim" > > Message-ID: <002401c87e20$e90325d0$6500a8c0@mainbox> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > Hi Tim, > > Thanks, I am aware of both papers and also a follow-up discussion at BSC > last year. > The authors also indicate that some epitopes do not mask or require HIER to > demonstrate, even though they have been 'fixed' for long enough. > This is of course born out by every day experience in the IHC lab. > Interestingly, they also point out that some peptides that are fixed this > way and do not require HIER, WILL require HIER if fixed in the presence of > another protein or peptide. > That of course is also the case in the real world of tissues!!! > > (See also Yang K, Sompuram SR, Fitzgibbons P Bogen SA. National HER2 > proficiency test results using standardized quantitative controls: > characterization of laboratory failures. > Arch Pathol Lab Med 112, February 2008 pp 211-216.) > > As you know, I have long decried the practice of short fixing small biopsies > and the effects of reversal and refixation by alcohol during processing. > This can be particularly devastating on surface proteins such as HER2 etc. > etc. > The 6 hour minimum fixation time in the HER2 guidelines is, in my opinion, > wrong!!! > OK, I'll step off the soap box. > > > cheers, > > Bryan > > > > > > > > ----- Original Message ----- > From: "Morken, Tim" > To: > Sent: Tuesday, March 04, 2008 12:16 PM > Subject: RE: [Histonet] Washing out formalin fixation (Lengthy) > > > > Thanks for the synopsis Bryan. > > I'd like to point out that the 2004 paper by Sompuram,and a followup in > 2006 are very interesting. These studies these show that fixation of > peptide spots (essentially zero thickness) attached to glass slides take > over 6 hours to "fix" at room temperature. In this case they defined > "fixed" as the point at which an antibody would no longer detect it's > target epitope, or the reaction was severly diminished. They also showed > that HIER reverses the "fixation." This calls into question any method > that relies on less than six hours formalin fixation at room temperature > (ie, biospies, just because they are small) and also the effect of > exposing those short-fixed tissues to long exposure to 70% alcohol, or > other aqueous solution, before clearing and embedding. > > The 2006 paper also investigates why some epitopes are affected by > formalin and others are not. > > Sompuram SR, Vani K, Messana E and Bogen SA. A molecular mechanism of > formalin fixation and antigen retrieval. Am J Clin Pathol (2004);121: > 190-199 > > Sompuram, et. al, A molecular model of antigen retrieval using a peptide > array, Am J Clin Path 2006;125:91-98 > > > Tim Morken > Technical Support Manager > Lab Vision Products > Anatomical Pathology > ThermoFisher Scientific > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan > Hewlett > Sent: Tuesday, March 04, 2008 7:56 AM > To: Johnson, Teri; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Washing out formalin fixation (Lengthy) > > Hi Teri and everyone else on this thread, > > Washing out many of the effects of formaldehyde fixation on tissues with > running water has been known for years (much longer than this old boy > has been around). > In modern terms, it is the essential underlying mechanism for so-called > antigen retrieval (HIER). > > Formaldehyde fixes proteins by addition, with the formation of > hydroxymethyl adducts on the reactive side chains of proteins. > Once enough of these hydroxymethyl adducts are formed, and IF they are > in close approximation to each other, they may slowly cross-link by the > formation of methylene bridges. > However, these adducts and initial cross-links are unstable and readily > reversed by water and alcohol (see Kiernan (1999). > It takes 24 hours at room temperature for all the hydroxymethyl adducts > to form, i.e. maximal binding threshold (see Fox et al, 1985). > If the tissue is then exposed to running water before all the adducts > have formed (i.e. less than 24 hours), the reversal is very rapid. > The shorter the time in formaldehyde, the more rapid the reversal. > Even after the essential 24 hours fixation and also after a more lengthy > 6 days fixation, running water will still remove the adducts and > hydrolyse the methylene bridges. > There is at least one publication (Helander, 1994) that provides data > regarding this effect. > After 24 hours fixation, 50% reversal occurred in less than 24 hours, > 90% reversal was obtained after 6 days washing and for 6 days fixation > 90% reversal after 4 weeks washing. > It should be noted that these reversal times were obtained at ambient > temperatures and the times may be considerably reduced by elevated > temperatures. > > This reversal effect is also obtained on tissue sections that have been > processed to wax. > However, because of the additional shrinkage and hydrophobicity of the > processed proteins, the reversal is slowed somewhat until the proteins > re-hydrate. > The reversal effect can also be aided by the presence of other ions in > the water (the purpose of HIER buffers). > Back in the sixties, in order to successfully demonstrate Ig's by IF, we > were reversing the fixation effects on paraffin sections by placing them > in hypotonic buffers for 2 days at 37C. > Today, since we are all in a great rush for results, we obviously drive > the reversal at higher temperatures to speed things up!! > > References: > > Baker JR (1958), Principles of Biological Microtechnique, Methuen & Co. > Ltd. > Hopwood D. Fixatives and fixation: A review. Histochemical journal > (1969); 1, p19-55 Burnett MG. The mechanism of the formaldehyde clock > reaction: Methylene glycol dehydration. J Chem. educ. (1982); 59, 160 > Fox CH et al. Formaldehyde fixation. J Histochem Cytochem (1985); 33, > 845-853 > Helander KG. Kinetic studies of formaldehyde binding in tissue. > Biotechnique and Histochemistry. (1994); 69, 177-179 Kiernan J.A., > Histological and Histochemical Methods: Theory and Practice, 3rd > Edition, (1999). Oxford: Butterworth-Heinemann. ISBN # 0-7506-3106-6. > Shi SR, Gu J. and Taylor CR. Antigen Retrieval Techniques: > Immunohistochemistry and Molecular Morphology. (2000) Eaton Publishing. > ISBN 1-881299-43-0. > Sompuram SR, Vani K, Messana E and Bogen SA. A molecular mechanism of > formalin fixation and antigen retrieval. Am J Clin Pathol (2004);121: > 190-199 > > > Best regards, > > Bryan > > ----- Original Message ----- > From: "Johnson, Teri" > To: > Sent: Monday, March 03, 2008 2:33 PM > Subject: [Histonet] Washing out formalin fixation > > > Last week, a researcher here asked me what the chemical mechanism was of > > washing out the effects of formalin fixation on the tissues with running > > water. In other words, how does it work? Anybody here know? > > Teri Johnson, HT(ASCP)QIHC > Managing Director Histology Facility > Stowers Institute for Medical Research > 1000 E. 50th St. > Kansas City, MO 64110 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 52, Issue 5 > *************************************** -- Melissa R. Mazan, DVM, Diplomate ACVIM Associate Professor and Director of Equine Sports Medicine Tufts Cummings School of Veterinary Medicine 200 Westboro Road North Grafton, MA 01536 Tel:508-839-5395 Email: melissa.mazan@tufts.edu Fax:508-839-7922 From bhewlett <@t> cogeco.ca Tue Mar 4 12:21:27 2008 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Tue Mar 4 12:21:36 2008 Subject: [Histonet] fixation times References: <200803041801.m24I1b5D024717@mail-proofpoint-2a.usg.tufts.edu> <47CD907C.1070106@tufts.edu> Message-ID: <004201c87e24$90f0ed10$6500a8c0@mainbox> Melissa, What type of section- cryo or paraffin? I hate to tell you this, but after more than 40 years of IHC experience with both animal and human tissues, I have yet to find an antibody for paraffin sections that does not work more consistently after a minimum of 24 hours fixation in formaldehyde. After only 4-6 hours formaldehyde fixation your paraffin tissues are essentially alcohol fixed in the processor! For some epitopes e.g. vimentin, cytokeratins and other intermediate filaments, that's fine, but for most cell surface proteins NOT. Bryan ----- Original Message ----- From: "Melissa Mazan" To: Sent: Tuesday, March 04, 2008 1:10 PM Subject: [Histonet] fixation times > This thread has been very interesting - and I have a practical question. > We do IHC - both immunofluorescent and immunoenzyme - on lung tissue in > our lab. We generally try to fix the tissues in formalin for at least 4 > hours and no more than 6 hours - as we tend to get better antibody > binding - this is even with HIER. Is there an optimum amount of time that > the group recommends? Thanks - Melissa > > histonet-request@lists.utsouthwestern.edu wrote: > >> Send Histonet mailing list submissions to >> histonet@lists.utsouthwestern.edu >> >> To subscribe or unsubscribe via the World Wide Web, visit >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> or, via email, send a message with subject or body 'help' to >> histonet-request@lists.utsouthwestern.edu >> >> You can reach the person managing the list at >> histonet-owner@lists.utsouthwestern.edu >> >> When replying, please edit your Subject line so it is more specific >> than "Re: Contents of Histonet digest..." >> >> >> Today's Topics: >> >> 1. RE: Washing out formalin fixation (Lengthy) >> (Marshall Terry Dr, Consultant Histopathologist) >> 2. What happened to BIOMEDA (Patricia Karlisch) >> 3. Re: Histonet Digest, Vol 52, Issue 4 (MKing) >> 4. Re: Washing out formalin fixation (Lengthy) (Bryan Hewlett) >> >> >> ---------------------------------------------------------------------- >> >> Message: 1 >> Date: Tue, 4 Mar 2008 17:35:04 -0000 >> From: "Marshall Terry Dr, Consultant Histopathologist" >> >> Subject: RE: [Histonet] Washing out formalin fixation (Lengthy) >> To: "Morken, Tim" , >> >> Message-ID: >> <5C0BED61F529364E86309CADEA63FEF20163F358@TRFT-EX01.xRothGen.nhs.uk> >> Content-Type: text/plain; charset="us-ascii" >> >> "These studies these show that fixation of peptide spots (essentially >> zero thickness) attached to glass slides take over 6 hours to "fix" at >> room temperature. In this case they defined "fixed" as the point at >> which an antibody would no longer detect it's target epitope, or the >> reaction was severely diminished. They also showed that HIER reverses >> the "fixation" >> >> So far so good, and supports what I have been saying in this forum for >> years about the (long) time it takes to formalin fix tissue, and the >> misbelief that wetting them for a few hours is fixing. >> But then...... >> >> "This calls into question any method that relies on less than six hours >> formalin fixation at room temperature (ie, biopsies, just because they >> are small) and also the effect of exposing those short-fixed tissues to >> long exposure to 70% alcohol, or other aqueous solution, before clearing >> and embedding." >> >> I know not what this all means/is getting at. >> >> Terry >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, >> Tim >> Sent: 04 March 2008 17:17 >> To: histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] Washing out formalin fixation (Lengthy) >> >> >> Thanks for the synopsis Bryan. >> >> I'd like to point out that the 2004 paper by Sompuram,and a followup in >> 2006 are very interesting. These studies these show that fixation of >> peptide spots (essentially zero thickness) attached to glass slides take >> over 6 hours to "fix" at room temperature. In this case they defined >> "fixed" as the point at which an antibody would no longer detect it's >> target epitope, or the reaction was severly diminished. They also showed >> that HIER reverses the "fixation." >> >> >> The 2006 paper also investigates why some epitopes are affected by >> formalin and others are not. >> >> Sompuram SR, Vani K, Messana E and Bogen SA. A molecular mechanism of >> formalin fixation and antigen retrieval. Am J Clin Pathol (2004);121: >> 190-199 >> >> Sompuram, et. al, A molecular model of antigen retrieval using a peptide >> array, Am J Clin Path 2006;125:91-98 >> >> >> Tim Morken >> Technical Support Manager >> Lab Vision Products >> Anatomical Pathology >> ThermoFisher Scientific >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan >> Hewlett >> Sent: Tuesday, March 04, 2008 7:56 AM >> To: Johnson, Teri; histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] Washing out formalin fixation (Lengthy) >> >> Hi Teri and everyone else on this thread, >> >> Washing out many of the effects of formaldehyde fixation on tissues with >> running water has been known for years (much longer than this old boy >> has been around). >> In modern terms, it is the essential underlying mechanism for so-called >> antigen retrieval (HIER). >> >> Formaldehyde fixes proteins by addition, with the formation of >> hydroxymethyl adducts on the reactive side chains of proteins. >> Once enough of these hydroxymethyl adducts are formed, and IF they are >> in close approximation to each other, they may slowly cross-link by the >> formation of methylene bridges. >> However, these adducts and initial cross-links are unstable and readily >> reversed by water and alcohol (see Kiernan (1999). >> It takes 24 hours at room temperature for all the hydroxymethyl adducts >> to form, i.e. maximal binding threshold (see Fox et al, 1985). >> If the tissue is then exposed to running water before all the adducts >> have formed (i.e. less than 24 hours), the reversal is very rapid. >> The shorter the time in formaldehyde, the more rapid the reversal. >> Even after the essential 24 hours fixation and also after a more lengthy >> 6 days fixation, running water will still remove the adducts and >> hydrolyse the methylene bridges. >> There is at least one publication (Helander, 1994) that provides data >> regarding this effect. >> After 24 hours fixation, 50% reversal occurred in less than 24 hours, >> 90% reversal was obtained after 6 days washing and for 6 days fixation >> 90% reversal after 4 weeks washing. >> It should be noted that these reversal times were obtained at ambient >> temperatures and the times may be considerably reduced by elevated >> temperatures. >> >> This reversal effect is also obtained on tissue sections that have been >> processed to wax. >> However, because of the additional shrinkage and hydrophobicity of the >> processed proteins, the reversal is slowed somewhat until the proteins >> re-hydrate. >> The reversal effect can also be aided by the presence of other ions in >> the water (the purpose of HIER buffers). >> Back in the sixties, in order to successfully demonstrate Ig's by IF, we >> were reversing the fixation effects on paraffin sections by placing them >> in hypotonic buffers for 2 days at 37C. >> Today, since we are all in a great rush for results, we obviously drive >> the reversal at higher temperatures to speed things up!! >> >> References: >> >> Baker JR (1958), Principles of Biological Microtechnique, Methuen & Co. >> Ltd. >> Hopwood D. Fixatives and fixation: A review. Histochemical journal >> (1969); 1, p19-55 Burnett MG. The mechanism of the formaldehyde clock >> reaction: Methylene glycol dehydration. J Chem. educ. (1982); 59, 160 >> Fox CH et al. Formaldehyde fixation. J Histochem Cytochem (1985); 33, >> 845-853 >> Helander KG. Kinetic studies of formaldehyde binding in tissue. >> Biotechnique and Histochemistry. (1994); 69, 177-179 Kiernan J.A., >> Histological and Histochemical Methods: Theory and Practice, 3rd >> Edition, (1999). Oxford: Butterworth-Heinemann. ISBN # 0-7506-3106-6. >> Shi SR, Gu J. and Taylor CR. Antigen Retrieval Techniques: >> Immunohistochemistry and Molecular Morphology. (2000) Eaton Publishing. >> ISBN 1-881299-43-0. >> Sompuram SR, Vani K, Messana E and Bogen SA. A molecular mechanism of >> formalin fixation and antigen retrieval. Am J Clin Pathol (2004);121: >> 190-199 >> >> >> Best regards, >> >> Bryan >> >> ----- Original Message ----- >> From: "Johnson, Teri" >> To: >> Sent: Monday, March 03, 2008 2:33 PM >> Subject: [Histonet] Washing out formalin fixation >> >> >> Last week, a researcher here asked me what the chemical mechanism was of >> >> washing out the effects of formalin fixation on the tissues with running >> >> water. In other words, how does it work? Anybody here know? >> >> Teri Johnson, HT(ASCP)QIHC >> Managing Director Histology Facility >> Stowers Institute for Medical Research >> 1000 E. 50th St. >> Kansas City, MO 64110 >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> ###################################################################### >> This email and any files transmitted with it may contain confidential and >> privileged information which is intended solely for the use of the >> individual or entity to whom they are addressed. Any views or opinions >> expressed are those of the author and do not represent the views of The >> Rotherham NHS Foundation Trust unless otherwise explicitly stated. >> The information contained in this email may be subject to public >> disclosure under the Freedom of Information Act 2000 unless the >> information is legally exempt from disclosure. The confidentiality of >> this email and your reply cannot be guaranteed. >> If you are not the intended recipient please accept our apologies. Please >> do not disclose, copy or distribute information in this email or take any >> action in reliance on its contents: to do so is strictly prohibited and >> may be unlawful. Please inform us that this message has gone astray >> before deleting it. Thank you for your co-operation. >> ###################################################################### >> >> >> ------------------------------ >> >> Message: 2 >> Date: Tue, 04 Mar 2008 12:43:42 -0500 >> From: "Patricia Karlisch" >> Subject: [Histonet] What happened to BIOMEDA >> To: >> Message-ID: <47CD43FD.07B7.008C.0@hmc.psu.edu> >> Content-Type: text/plain; charset=US-ASCII >> >> Histonetters, >> I can not contact Biomeda any longer. Does anyone know what >> happened? Thanks, Pat >> Pat Karlisch >> Supervisor, Histology, Pathology and Laboratory Medicine >> Penn State Milton S. Hershey Medical Center >> Mail Code H179 >> Hershey, PA 17033 >> Phone (717) 531-6072 >> Fax: (717) 531- 7741 >> email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** >> This message (including any attachments) contains information intended >> for a specific individual(s) and purpose that may be privileged, >> confidential or otherwise protected from disclosure pursuant to >> applicable law. Any inappropriate use, distribution or copying of the >> message is strictly prohibited and may subject you to criminal or civil >> penalty. If you have received this transmission in error, please reply >> to the sender indicating this error and delete the transmission from your >> system immediately. >> >> >> ------------------------------ >> >> Message: 3 >> Date: Tue, 04 Mar 2008 12:48:16 -0500 >> From: MKing >> Subject: [Histonet] Re: Histonet Digest, Vol 52, Issue 4 >> To: histonet@lists.utsouthwestern.edu >> Message-ID: <47CD8B60.3030101@ufl.edu> >> Content-Type: text/plain; charset=ISO-8859-1; format=flowed >> >> Bryan and Tim, >> Thanks for excellent postsMK, this info will go in my tech folder! It >> looked like this thread was headed into a flame war, nice to have >> substantiated data prevail here. >> Mike King >> UF Pharmacology & Therapeutics >> -------------------- >> Date: Tue, 4 Mar 2008 10:56:18 -0500 >> From: "Bryan Hewlett" >> Subject: Re: [Histonet] Washing out formalin fixation (Lengthy) >> >> >> >> >> ------------------------------ >> >> Message: 4 >> Date: Tue, 4 Mar 2008 12:55:19 -0500 >> From: "Bryan Hewlett" >> Subject: Re: [Histonet] Washing out formalin fixation (Lengthy) >> To: , "Morken, Tim" >> >> Message-ID: <002401c87e20$e90325d0$6500a8c0@mainbox> >> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; >> reply-type=original >> >> Hi Tim, >> >> Thanks, I am aware of both papers and also a follow-up discussion at BSC >> last year. >> The authors also indicate that some epitopes do not mask or require HIER >> to demonstrate, even though they have been 'fixed' for long enough. >> This is of course born out by every day experience in the IHC lab. >> Interestingly, they also point out that some peptides that are fixed this >> way and do not require HIER, WILL require HIER if fixed in the presence >> of another protein or peptide. >> That of course is also the case in the real world of tissues!!! >> >> (See also Yang K, Sompuram SR, Fitzgibbons P Bogen SA. National HER2 >> proficiency test results using standardized quantitative controls: >> characterization of laboratory failures. >> Arch Pathol Lab Med 112, February 2008 pp 211-216.) >> >> As you know, I have long decried the practice of short fixing small >> biopsies and the effects of reversal and refixation by alcohol during >> processing. >> This can be particularly devastating on surface proteins such as HER2 >> etc. etc. >> The 6 hour minimum fixation time in the HER2 guidelines is, in my >> opinion, wrong!!! >> OK, I'll step off the soap box. >> >> >> cheers, >> >> Bryan >> >> >> >> >> >> >> >> ----- Original Message ----- >> From: "Morken, Tim" >> To: >> Sent: Tuesday, March 04, 2008 12:16 PM >> Subject: RE: [Histonet] Washing out formalin fixation (Lengthy) >> >> >> >> Thanks for the synopsis Bryan. >> >> I'd like to point out that the 2004 paper by Sompuram,and a followup in >> 2006 are very interesting. These studies these show that fixation of >> peptide spots (essentially zero thickness) attached to glass slides take >> over 6 hours to "fix" at room temperature. In this case they defined >> "fixed" as the point at which an antibody would no longer detect it's >> target epitope, or the reaction was severly diminished. They also showed >> that HIER reverses the "fixation." This calls into question any method >> that relies on less than six hours formalin fixation at room temperature >> (ie, biospies, just because they are small) and also the effect of >> exposing those short-fixed tissues to long exposure to 70% alcohol, or >> other aqueous solution, before clearing and embedding. >> >> The 2006 paper also investigates why some epitopes are affected by >> formalin and others are not. >> >> Sompuram SR, Vani K, Messana E and Bogen SA. A molecular mechanism of >> formalin fixation and antigen retrieval. Am J Clin Pathol (2004);121: >> 190-199 >> >> Sompuram, et. al, A molecular model of antigen retrieval using a peptide >> array, Am J Clin Path 2006;125:91-98 >> >> >> Tim Morken >> Technical Support Manager >> Lab Vision Products >> Anatomical Pathology >> ThermoFisher Scientific >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan >> Hewlett >> Sent: Tuesday, March 04, 2008 7:56 AM >> To: Johnson, Teri; histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] Washing out formalin fixation (Lengthy) >> >> Hi Teri and everyone else on this thread, >> >> Washing out many of the effects of formaldehyde fixation on tissues with >> running water has been known for years (much longer than this old boy >> has been around). >> In modern terms, it is the essential underlying mechanism for so-called >> antigen retrieval (HIER). >> >> Formaldehyde fixes proteins by addition, with the formation of >> hydroxymethyl adducts on the reactive side chains of proteins. >> Once enough of these hydroxymethyl adducts are formed, and IF they are >> in close approximation to each other, they may slowly cross-link by the >> formation of methylene bridges. >> However, these adducts and initial cross-links are unstable and readily >> reversed by water and alcohol (see Kiernan (1999). >> It takes 24 hours at room temperature for all the hydroxymethyl adducts >> to form, i.e. maximal binding threshold (see Fox et al, 1985). >> If the tissue is then exposed to running water before all the adducts >> have formed (i.e. less than 24 hours), the reversal is very rapid. >> The shorter the time in formaldehyde, the more rapid the reversal. >> Even after the essential 24 hours fixation and also after a more lengthy >> 6 days fixation, running water will still remove the adducts and >> hydrolyse the methylene bridges. >> There is at least one publication (Helander, 1994) that provides data >> regarding this effect. >> After 24 hours fixation, 50% reversal occurred in less than 24 hours, >> 90% reversal was obtained after 6 days washing and for 6 days fixation >> 90% reversal after 4 weeks washing. >> It should be noted that these reversal times were obtained at ambient >> temperatures and the times may be considerably reduced by elevated >> temperatures. >> >> This reversal effect is also obtained on tissue sections that have been >> processed to wax. >> However, because of the additional shrinkage and hydrophobicity of the >> processed proteins, the reversal is slowed somewhat until the proteins >> re-hydrate. >> The reversal effect can also be aided by the presence of other ions in >> the water (the purpose of HIER buffers). >> Back in the sixties, in order to successfully demonstrate Ig's by IF, we >> were reversing the fixation effects on paraffin sections by placing them >> in hypotonic buffers for 2 days at 37C. >> Today, since we are all in a great rush for results, we obviously drive >> the reversal at higher temperatures to speed things up!! >> >> References: >> >> Baker JR (1958), Principles of Biological Microtechnique, Methuen & Co. >> Ltd. >> Hopwood D. Fixatives and fixation: A review. Histochemical journal >> (1969); 1, p19-55 Burnett MG. The mechanism of the formaldehyde clock >> reaction: Methylene glycol dehydration. J Chem. educ. (1982); 59, 160 >> Fox CH et al. Formaldehyde fixation. J Histochem Cytochem (1985); 33, >> 845-853 >> Helander KG. Kinetic studies of formaldehyde binding in tissue. >> Biotechnique and Histochemistry. (1994); 69, 177-179 Kiernan J.A., >> Histological and Histochemical Methods: Theory and Practice, 3rd >> Edition, (1999). Oxford: Butterworth-Heinemann. ISBN # 0-7506-3106-6. >> Shi SR, Gu J. and Taylor CR. Antigen Retrieval Techniques: >> Immunohistochemistry and Molecular Morphology. (2000) Eaton Publishing. >> ISBN 1-881299-43-0. >> Sompuram SR, Vani K, Messana E and Bogen SA. A molecular mechanism of >> formalin fixation and antigen retrieval. Am J Clin Pathol (2004);121: >> 190-199 >> >> >> Best regards, >> >> Bryan >> >> ----- Original Message ----- >> From: "Johnson, Teri" >> To: >> Sent: Monday, March 03, 2008 2:33 PM >> Subject: [Histonet] Washing out formalin fixation >> >> >> Last week, a researcher here asked me what the chemical mechanism was of >> >> washing out the effects of formalin fixation on the tissues with running >> >> water. In other words, how does it work? Anybody here know? >> >> Teri Johnson, HT(ASCP)QIHC >> Managing Director Histology Facility >> Stowers Institute for Medical Research >> 1000 E. 50th St. >> Kansas City, MO 64110 >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> >> ------------------------------ >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> End of Histonet Digest, Vol 52, Issue 5 >> *************************************** > > -- > Melissa R. Mazan, DVM, Diplomate ACVIM > Associate Professor and Director of Equine Sports Medicine > Tufts Cummings School of Veterinary Medicine > 200 Westboro Road > North Grafton, MA 01536 > Tel:508-839-5395 > Email: melissa.mazan@tufts.edu > Fax:508-839-7922 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From RSRICHMOND <@t> aol.com Tue Mar 4 13:10:11 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Tue Mar 4 13:10:18 2008 Subject: [Histonet] Re: Coombs test Message-ID: Kathy Cormier asks: >>I was looking to run a Coombs Test for a Primate sample...<< The Coombs test (direct antiglobulin test, DAT) looks for agglutination of antibody-coated red cells by an agglutinating antibody directed against the primary antibody that's on the patient's red cells. If the patient is a monkey, you cannot assume that an agglutinating antibody against human immunoglobulin will produce a positive result. If the patient is an Anglican bishop, you're probably OK. Bob Richmond Samurai Pathologist and occasional Episcopalian Knoxville TN From POWELL_SA <@t> Mercer.edu Tue Mar 4 13:14:39 2008 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Tue Mar 4 13:19:59 2008 Subject: [Histonet] Region III Hosted by Georgia Society for Histotechnology REMINDER Message-ID: <01MS0YR8Y96W000N4P@Macon2.Mercer.edu> Hi Guys, The Westin has extended the deadline for registration for the Region III meeting to be held in Atlanta Georgia April 3-5, 2008 at the Westin Peachtree Plaza Hotel. The discounted rate is $129 single, double, triple or quad. The phone number to the Westin is 1-404-659-1400 and please state that you are attending the GSH/Region III meeting. Don't let the deadline sneak up on you. The revised program can be downloaded from our website at www.histosearch.com/gsh. Click on the symposium link to get a PDF file of the program. Vendors have a link to their registration form to exhibit at the meeting on the same page. If you have questions Chris Coley, GSH Exhibit Liaison, has contact information is on that form If anyone has any questions please feel to contact me at this email address. Come on down to Georgia, it was in the mid 70s yesterday. Shirley Powell GSH Secretary/Registrar From CIngles <@t> uwhealth.org Tue Mar 4 13:21:48 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Tue Mar 4 13:22:00 2008 Subject: [Histonet] Liquid Nitrogen guns Message-ID: <08A0A863637F1349BBFD83A96B27A50A120102@uwhis-xchng3.uwhis.hosp.wisc.edu> Hey gang: Sorry it has taken me so long to get back to you with the info. It's been crazy here for the past couple weeks. Anyway, here goes. We order our Liquid Nitrogen guns from Brymill. They are out of Ellington CT 06029. Their number is 800-777-CRYO. They are the guns that are also usually used in Dermatology clinics. We also have a couple of 2 gallon Dewars flasks to hold the LN after we dispense it from the bigger 50L tank. I'm not sure where the large cryo tanks or Dewars flasks can be gotten. I believe there are actually smaller 25 L cryo tanks as well. We get the actual LN from a medical gas company (Airgas). The gun works essentially like the cryocool cans, but freezing of specific areas is much easier. The fatty areas can be targeted and the more fiberous/muscle areas and OCT/TBS can be avoided so they are not over cooled and become friable. We regularly cut whole 2x2 cm pieces of pure fat with great results. It takes a bit longer, but there is actually tissue on the slide instead of a hole or just a blob of fat. I'll try and help with any questions anyone has.(I'll try to respond to you faster next time too). Claire Ingles UW Hospital and Clinics Madison WI From JBower <@t> hei.org Tue Mar 4 14:40:58 2008 From: JBower <@t> hei.org (Bower, Jennifer) Date: Tue Mar 4 14:43:52 2008 Subject: [Histonet] Coombs Tests References: Message-ID: <87449E4A2B01DA47B29424CE5D6E0F8307AAFDF9@hi0sml1.hei.org> We did Coombs tests on animals at the vet clinic I worked at. Check out places like IDEXX, too for information. Jennifer Bower House Ear Institute P30 Histotechnician (213)989-7460 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jennifer MacDonald Sent: Tue 3/4/2008 9:09 AM To: Kathy Cormier Cc: histonet-bounces@lists.utsouthwestern.edu; histonet@pathology.swmed.edu Subject: Re: [Histonet] Coombs Tests Any blood bank laboratory will run a Coombs test. Try a local hospital. If you have a CLS/MT program at the school they should also be able to do this also, or have the contacts to have it done. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Kathy Cormier" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/04/2008 07:07 AM To cc Subject [Histonet] Coombs Tests I was looking to run a Coombs Test for a Primate sample, any suggestions on who is running this? Any suggestions would be appreciated. Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From garsha <@t> itg.uiuc.edu Tue Mar 4 15:39:07 2008 From: garsha <@t> itg.uiuc.edu (Karl Garsha) Date: Tue Mar 4 15:39:16 2008 Subject: [Histonet] removing cryoprotectant from fixed tissue Message-ID: <47CDC17B.6040705@itg.uiuc.edu> Greetings, I'm trying to help someone who has some formalin-fixed tissue sections that have been cryoprotected in a 30% ethylene glycol/20% glycerol solution; we'd like to process the tissue for laser microdissection for purposes of RNA extraction. The difficulty is that cryoprotectant tends to absorb the laser wavelengths and heat the tissue during the cutting process, causing damage and decreasing the liklihood of successful RNA extraction (the likelihood of which has already been reduced by the fixation process). I would imagine that there must be a way to de-cryoprotect through successive washes in a miscible solvent, I'm not sure if this would cause even greater complications with RNA work, or if fairly gentle standard protocols exist so I'm appealing to the collective knowledge of this list:) Has anyone experienced a need to remove cryoprotectant from tissue (brain) sections and are there perhaps methods to accomplish this that might be somewhat amenable to later extraction of RNA from select features in the tissue sections? Thanks in advance for any advice or helpful hints. Sincere Regards, Karl -- Karl Garsha Research Microscopy Specialist US-Southwest Region Leica Microsystems-Life Sciences Research www.leica-microsystems.com From Margaret.Perry <@t> sdstate.edu Tue Mar 4 15:46:15 2008 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Tue Mar 4 15:46:21 2008 Subject: [Histonet] Tris buffer Message-ID: We need to update our SOP's and I can't find a reference for our tris buffer recipe. I can find many recipes but not ours. Our recipe is as follows: 6 grams Trizma Hydrochloride 1.4 grams Trizma base 8.75 grams Sodium chloride Qs with water to 1 liter Do any of you have a reference for this recipe? Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 From Ronald.Houston <@t> nationwidechildrens.org Tue Mar 4 15:55:03 2008 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Tue Mar 4 15:55:38 2008 Subject: [Histonet] british article Message-ID: <979FF5962E234F45B06CF0DB7C1AABB215BCC91B@chi2k3ms01.columbuschildrens.net> Could someone from the UK get me a copy of the following article please? Smith VV , Milla PJ. Argyrophilia in the developing myenteric plexus. Br J Biomed Sci 1996;53:278-83. Thanks Ronnie Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 Ronald.Houston@NationwideChildrens.org Columbus Children's Hospital is now Nationwide Children's Hospital www.NationwideChildrens.org ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From POWELL_SA <@t> Mercer.edu Tue Mar 4 17:03:04 2008 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Tue Mar 4 17:07:36 2008 Subject: [Histonet] GSH meeting Message-ID: <01MS16QFKE0E000MMJ@Macon2.Mercer.edu> Hi Guys, Just wanted to clarify the workshop fees on our Region III/GSH program. Registrations I am receiving tell me it was not stated clearly. I will have a revised copy on our website in a day or so at www.histosearch.com/gsh. The fee is $40 per workshop for members, $55 per workshop for non-members, and $25 per workshop for students. Also one can only take three workshops in all, not all 5, because 1 and 2 run concurrently, as does 4 and 5. Thanks for your patience. Shirley Powell GSH Secretary/Registrar From Malcolm.McCallum <@t> tamut.edu Tue Mar 4 21:03:51 2008 From: Malcolm.McCallum <@t> tamut.edu (Malcolm McCallum) Date: Tue Mar 4 21:07:53 2008 Subject: [Histonet] Dewar question References: <01MS16QFKE0E000MMJ@Macon2.Mercer.edu> Message-ID: Hello, My question has little to do with histology, but hopefully someone on here has a clue in my dilemna. I am interested in getting a Dewar for holding straws of livestock semen in! The bottom line is that Dewars are either holding hundreds of straws which is way larger than what i need. then tehre are numerous dewars that are for storing liquid N. The most I might have is 5-10 straws at any given time, and I'm only looking to store for a month or two (waiting for goats to go in heat). So, any advice? I've been watching the used dewars on various sites, but it seems that none that specifically say they are for semen straws go for less than $300! Thanks for the spot of help! :) Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-223-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html VISIT HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org A New Journal Published in Partnership with Partners in Amphibian and Reptile Conservation and the World Congress of Herpetology. Spring Teaching Schedule & Office Hours: Genetics: W 6:00 to 9:40pm Herpetology: TR 10:00-11:40am Histology: MW 1:00-2:40pm Seminar: T 2:30-3:30pm Office Hours: M: 3:30-5:00pm T: 11:40-1:00pm; 3:30-5:00pm W: 4:00-6:00pm "We live in a time when lemonade is made with artificial flavoring, and furnisher polish is made with fresh lemons." -Alfred E. Neuman -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Shirley Powell Sent: Tue 3/4/2008 5:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GSH meeting Hi Guys, Just wanted to clarify the workshop fees on our Region III/GSH program. Registrations I am receiving tell me it was not stated clearly. I will have a revised copy on our website in a day or so at www.histosearch.com/gsh. The fee is $40 per workshop for members, $55 per workshop for non-members, and $25 per workshop for students. Also one can only take three workshops in all, not all 5, because 1 and 2 run concurrently, as does 4 and 5. Thanks for your patience. Shirley Powell GSH Secretary/Registrar _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Wed Mar 5 08:43:43 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed Mar 5 08:43:56 2008 Subject: [Histonet] Cardboard slide dividers in pretty colors In-Reply-To: <8CA4B503FEE9F09-654-2A55@MBLK-M07.sysops.aol.com> Message-ID: OK - I bought about 1,000 cardboard slide dividers, pink and blue (for girls and boys) - and for the life of me, I can't remember where I bought them. I know I'm getting old a feeble - does someone have a source? Thanks. Jackie O' From Susan.Ferrigon <@t> sanofi-aventis.com Wed Mar 5 09:07:39 2008 From: Susan.Ferrigon <@t> sanofi-aventis.com (Susan.Ferrigon@sanofi-aventis.com) Date: Wed Mar 5 09:07:52 2008 Subject: [Histonet] LAMP-2 Message-ID: <90B6684A9D6DAF468F7A5DC148754E1DA2E9AD@ALPW31.f2.enterprise> Hi Does any one have any experience staining LAMP-2 in rats?? I have a good method from a Pathology paper however it is using a Ventana autostainer, unfortunate we don't use autostainers, so need a manual protocol. Many thanks Susan From talulahgosh <@t> gmail.com Wed Mar 5 09:07:57 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Mar 5 09:08:05 2008 Subject: [Histonet] biomedia gel mount Message-ID: Hello everyone I ordered Biomedia Gel Mount from EMS through Fisher (their direct catalog number for Biomedia was missing) and it has a different label and is made by EMS. I'm going to use it since buying Biomedia stuff is no longer an option, I'll let you guys know if it seems different. Emily -- People aren't like chocolates. People are bastards. Bastards with bastard coating and bastard filling. From rmweber113 <@t> comcast.net Wed Mar 5 09:25:15 2008 From: rmweber113 <@t> comcast.net (rmweber113@comcast.net) Date: Wed Mar 5 09:25:19 2008 Subject: [Histonet] FW: NY histology position Message-ID: <030520081525.27457.47CEBB5B000A4CB000006B412216527966CCCECE9D0A0D0A99039D@comcast.net> -------------- Forwarded Message: -------------- From: rmweber113@comcast.net To: histonet@lists.utsouthwestern.edu Subject: NY histology position Date: Mon, 03 Mar 2008 15:46:51 +0000 I have a position for a Histologist available at an established GI group located in New City, NY, Rockland County. ASCP certification required. NY state licence NOT required. Candidate should have the knowledge to set up and maintain a state of the art facility. Knowledge of grossing, processing and routine histology of gastric biopsies required. $35.00+/hr plus benefits. Flexable hours. No evenings, weekends or holidays. Qualified candidates can email me at rmweber113@comcast.net or call me at 732 814-1170. $500.00 for a successful referral and employer approved committed for 90 days. Marilynn Weber H.T.(ASCP)QIHC Twincrest From akbitting <@t> geisinger.edu Wed Mar 5 10:48:37 2008 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Wed Mar 5 10:48:59 2008 Subject: [Histonet] H&E control slide Message-ID: <47CE8895.2B7F.00C9.0@geisinger.edu> What is the consensus on what tissue to use for an H&E control. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From TJJ <@t> Stowers-Institute.org Wed Mar 5 11:09:30 2008 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Wed Mar 5 11:10:01 2008 Subject: [Histonet] Re: H&E control slide Message-ID: Angela, I don't know if there is a consensus, but one lab I know used a piece of appendix. It's a good idea because there is a fair amount of material left over from surgical samples available for use. There are also epithelial cells, muscle tissue, and some lymphoid cells represented. You could also combine a few different tissue types together in a block and use that. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From tanisha.mcknight <@t> covance.com Wed Mar 5 11:10:41 2008 From: tanisha.mcknight <@t> covance.com (McKnight, Tanisha) Date: Wed Mar 5 11:10:53 2008 Subject: [Histonet] Tissue Slide Storage - Antigen, DNA & RNA Preservation Message-ID: <816E3C72F855F14985FC31D7C963AE6F05051BFC@indexch03.ent.covance.com> Hello All: I am interested in exploring different methods of storing (long term) and shipping tissue slides for future IHC or Molecular Studies. So far, I've found that paraffin dipping and refrigeration is popular. But I've also heard of labs using nitrogen in ambient storage units in conjunction with paraffin dipping. Or some using nitrogen, refrigeration and paraffin dipping. The slides were then shipped in vacuum sealed bags. If anyone has experience with these methods, do you mind sharing them with me. Thanks. Tanisha N. McKnight, HT (ASCP) Specimen Management, Anatomic Pathology ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From Terry.Marshall <@t> rothgen.nhs.uk Wed Mar 5 11:20:36 2008 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Wed Mar 5 11:20:16 2008 Subject: [Histonet] Re: H&E control slide Message-ID: <5C0BED61F529364E86309CADEA63FEF20163F366@TRFT-EX01.xRothGen.nhs.uk> I would use whatever it is in your lab. that stains most consistently badly. Everywhere I've been that is endometrium. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Teri Sent: 05 March 2008 17:10 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Re: H&E control slide Angela, I don't know if there is a consensus, but one lab I know used a piece of appendix. It's a good idea because there is a fair amount of material left over from surgical samples available for use. There are also epithelial cells, muscle tissue, and some lymphoid cells represented. You could also combine a few different tissue types together in a block and use that. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ###################################################################### This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. ###################################################################### From Barry.R.Rittman <@t> uth.tmc.edu Wed Mar 5 11:20:07 2008 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Wed Mar 5 11:20:18 2008 Subject: [Histonet] Re: H&E control slide In-Reply-To: References: Message-ID: Angela I would strongly suggest that you use tissue of the type you are going to study. I would agree that a generic tissue with epithelium, connective tissue and muscle is good for many things. There are however a lot of differences in the inherent staining of different organ, liver cells in general for example will have a more basophilic cytoplasm than many other epithelial cells. Additionally, many pathologists will have individual preferences for intensity of eosin staining and if the slide is to be photographed. Barry From b-frederick <@t> northwestern.edu Wed Mar 5 11:42:48 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Mar 5 11:42:51 2008 Subject: [Histonet] H&E control slide In-Reply-To: <47CE8895.2B7F.00C9.0@geisinger.edu> Message-ID: <000001c87ee8$57a1e7c0$d00f7ca5@lurie.northwestern.edu> We use tonsil or other lymphoid tissue- if it does not stain as it should (where we can see the nucleus) we know somethings up. ernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Wednesday, March 05, 2008 10:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E control slide What is the consensus on what tissue to use for an H&E control. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From tkngflght <@t> yahoo.com Wed Mar 5 11:21:00 2008 From: tkngflght <@t> yahoo.com (Cheryl) Date: Wed Mar 5 11:52:17 2008 Subject: [Histonet] H&E control slide In-Reply-To: <47CE8895.2B7F.00C9.0@geisinger.edu> References: <47CE8895.2B7F.00C9.0@geisinger.edu> Message-ID: <004d01c87ee5$48ab8530$6901a8c0@FSROGER> Hi Angela- You want to cover the full range of cell types and fixatives you'll be running through the stain--so this may vary by lab. If you use alternate fixatives on your sm bowel bx like Hollandes, you'll want to get some leftover fresh bowel from a resection or autopsy and keep a stock of this fixed in the alternate primary. Include a formalin piece and any thing else with a wide range of cell types so you can catch any issues with your control before staining a rack. Skin with a piece of bowel (NBF or your alternate fixative--or both would be better) covers the full range of demonstrable cell types and gives enough information to be sure the pH is where it should be (no 'robins egg blue' staining in the mucin). To complete the loop, make sure to document the standard in your procedure manual and include the QC verification on everyone's proficiency check sheet if it's not already there. Hope this helps! Cheryl Cheryl R. Kerry, HT(ASCP), BA Full Staff Inc. Staffing Allied Health Professionals - One GREAT tech at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone admin@fullstaff.org www.fullstaff.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Wednesday, March 05, 2008 10:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E control slide What is the consensus on what tissue to use for an H&E control. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From CBark <@t> memorialcare.org Wed Mar 5 12:17:10 2008 From: CBark <@t> memorialcare.org (Christine Bark) Date: Wed Mar 5 12:17:29 2008 Subject: [Histonet] RE: Cardboard slide dividers in pretty colors References: <6BD0100A1S41693918-01@emf1.memorialcare.org> Message-ID: You can get them at Lab Storage Systems ( www.labstore.com ). They're called slide index markers. Christine Bark, HT (ASCP) Sr. Histotech, Pathology Saddleback Memorial Medical Center 949-452-3548 cbark@memorialcare.org --------------------- Message: 13 Date: Wed, 5 Mar 2008 08:43:43 -0600 From: Jackie M O'Connor Subject: [Histonet] Cardboard slide dividers in pretty colors To: histonet@pathology.swmed.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" OK - I bought about 1,000 cardboard slide dividers, pink and blue (for girls and boys) - and for the life of me, I can't remember where I bought them. I know I'm getting old a feeble - does someone have a source? Thanks. Jackie O' ______________________________________________________________________________ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Erin.Martin <@t> ucsf.edu Wed Mar 5 12:43:27 2008 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Wed Mar 5 12:45:08 2008 Subject: [Histonet] CD246 ALK control slides Message-ID: Hi everyone, Does anyone know of a vendor for CD246 ALK protein control slides? Thanks Erin Martin From tkngflght <@t> yahoo.com Wed Mar 5 11:33:24 2008 From: tkngflght <@t> yahoo.com (Cheryl) Date: Wed Mar 5 12:55:50 2008 Subject: [Histonet] Permanent positions GALORE!! Message-ID: <005101c87ee7$04ad98d0$6901a8c0@FSROGER> Hi All-- We have a record number of permanent openings of all sorts. Entry level, bench, lead, supervisor, manager, research and specialty openings are all available. Please call for more information--some of these are exclusive Full Staff postings, most are due to growth! We know a LOT about the environments and people already working in these labs. Don't worry--if you don't have a resume or need to update it, we can write one with you. We want a good fit for both you and the lab, so a resume is the begining step to creating a happy match. Interview coaching, good listening skills (we use our mouth and ears in proportion), compensation negotiation and State license support are all part of our personal service. We never lose sight of the fact that it's your career --it's your life . We're here to help you find a job that you LOVE! Alaska Arizona - multiple positions and some research situations California - Central and South Colorado Connecticut Delaware Florida - license application support Georgia Indiana Massechusetts - Maryland Michigan Missouri New Jersey Nevada New York - help with this new license process North Carolina Ohio Oregon Pennsylvania South Carolina - IHC skills needed for one of these positions Tennessee Texas - many openings--places I like to work, too! Utah Virginia Washington - very desireable locations Washington DC The introduction conversation is with a histotech who knows what it's like to walk in your shoes~~ could be FUN!! Cheryl Cheryl R. Kerry, HT(ASCP), BA Full Staff Inc. Staffing Allied Health Professionals - One GREAT tech at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone resume@fullstaff.org admin@fullstaff.org www.fullstaff.org From jstaruk <@t> masshistology.com Wed Mar 5 13:05:58 2008 From: jstaruk <@t> masshistology.com (jstaruk) Date: Wed Mar 5 13:06:10 2008 Subject: [Histonet] CD31 vs. Von Willebrand Factor In-Reply-To: Message-ID: Hello all, I have a request for a CD31 looking for angiogenesis. I know this is a very problematic antibody when working with paraffin sections. Can the Van Willebrand antibody be substituted to obtain the same results? Thank you Jim _____________________ Jim Staruk Mass Histology Service www.masshistology.com From marktarango <@t> gmail.com Wed Mar 5 14:38:26 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Mar 5 14:38:37 2008 Subject: [Histonet] CD31 vs. Von Willebrand Factor In-Reply-To: References: Message-ID: <5b6eb13e0803051238u36d15783m1a1f0dbb65e709e8@mail.gmail.com> You're right that both of these antibodies will stain blood vessels. Depending on what's going on (what disease or process is occuring), you may find that these two antibodies don't always stain the same exact cells. I know that sometimes there is problem using CD31 on mouse tissues... is that what you need to do? If you're staining human tissue, I'd suggest using CD31 (since that's what the researcher/doc asked for). If its mouse tissue that you're dealing with, maybe someone on the list can suggest a CD31 antibody that they know definately works on mouse tissue. Let us know what kind of tissue and from what species you're staining, and I'm sure you'll get more specific help. On Wed, Mar 5, 2008 at 11:05 AM, jstaruk wrote: > Hello all, > y > I have a request for a CD31 looking for angiogenesis. I know this is a > very > problematic antibody when working with paraffin sections. Can the Van > Willebrand antibody be substituted to obtain the same results? > > Thank you > > Jim > > _____________________ > Jim Staruk > Mass Histology Service > www.masshistology.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From paul.kammeyer <@t> comphealth.com Wed Mar 5 15:20:28 2008 From: paul.kammeyer <@t> comphealth.com (paul.kammeyer@comphealth.com) Date: Wed Mar 5 15:20:45 2008 Subject: [Histonet] Attention NY Histotechs!! Message-ID: Hello Histonetters! I wanted to ask if there are any New York licensed histotechs out there who are available for permanent placement and/or contract assignments. Right now we are working with several different facilities who are in need of some good techs. If you are available I would love to hear from you! Also, refer your other friends with New York licenses for our jobs and you could get our referral bonus. That's an easy way for you to make some extra money. We will pay for the housing and travel. We also offer great benefits and pay. Let me know if you or anyone is interested. Call me or email me. Thanks so much! Paul Kammeyer CompHealth Staffing Consultant Phone: (800) 447-6016 ext. 3380 paul.kammeyer@comphealth.com www.comphealth.com Ask me about our $500 Referral Bonus Program!! From dellav <@t> musc.edu Wed Mar 5 15:28:37 2008 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Wed Mar 5 15:28:07 2008 Subject: [Histonet] potential HIPPA issue, would like your opinion In-Reply-To: <005101c87ee7$04ad98d0$6901a8c0@FSROGER> References: <005101c87ee7$04ad98d0$6901a8c0@FSROGER> Message-ID: Couriers delivering surgical or biopsy samples to our laboratory are asked to log in each sample they are delivering in a written record contained on a clipboard at the pathology receiving window at our facility. This occurs before any pathology staff actually accession the specimen and allows for an independent record completed by the courier of exactly what was delivered. An individual has contacted our Compliance office out of concern that because the record includes the patient's name, as well as other info including specimen, date, name of courier, etc, this information would be seen by subsequent couriers logging into this record. The record I'm referring to has on occasion assisted us in investigating when a clinician is looking for a report and we discover that the specimen was not received. Between our record and those maintained in the OR, for example, we can piece together the circumstances if need be. I hope that some of you will share the process you use, especially if your procedure might be more effective in maintaining the privacy of the patient. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Ph: (843) 792-6353 Fax: (843) 792-8974 From dellav <@t> musc.edu Wed Mar 5 15:43:08 2008 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Wed Mar 5 15:42:32 2008 Subject: [Histonet] Histology Supervisor Position NY In-Reply-To: <022920081741.23695.47C843E000002D0500005C8F2215586394CCCECE9D0A0D0A99039D@comcast.net> References: <022920081741.23695.47C843E000002D0500005C8F2215586394CCCECE9D0A0D0A99039D@comcast.net> Message-ID: Marilyn, I'm curious, how are individuals qualifying to work with the licensure requirements in your state? My understanding is that individuals who did not grandfather in now must have a med tech education in order to work in histology. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Ph: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of rmweber113@comcast.net Sent: Friday, February 29, 2008 12:42 PM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] Histology Supervisor Position NY -------------- Forwarded Message: -------------- From: rmweber113@comcast.net To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology Supervisor Position NY Date: Wed, 27 Feb 2008 16:38:58 +0000 I have a position for a Histology Supervisor available at an established GI group located in New City, NY, Rockland County. Candidate should have the knowledge to set up and maintain a state of the art facility. Knowledge of grossing, processing and routine histology of gastric biopsies required. $30.00/hr plus benefits. Qualified candidates can email me at rmweber113@comcast.net or call me at 732 814-1170 $500.00 for a successful referals committed for at least 90 days. Marilynn Weber H.T. (ASCP) QIHC Twincrest _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Mar 5 15:47:00 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 5 15:47:09 2008 Subject: [Histonet] potential HIPPA issue, would like your opinion In-Reply-To: Message-ID: <46982.64490.qm@web65702.mail.ac4.yahoo.com> We used a SINGLE form per patient that was deposited in a box. The delivery person filled the data in the form and placed it in the box, inaccessible to the next courier. Ren? J. "Della Speranza, Vinnie" wrote: Couriers delivering surgical or biopsy samples to our laboratory are asked to log in each sample they are delivering in a written record contained on a clipboard at the pathology receiving window at our facility. This occurs before any pathology staff actually accession the specimen and allows for an independent record completed by the courier of exactly what was delivered. An individual has contacted our Compliance office out of concern that because the record includes the patient's name, as well as other info including specimen, date, name of courier, etc, this information would be seen by subsequent couriers logging into this record. The record I'm referring to has on occasion assisted us in investigating when a clinician is looking for a report and we discover that the specimen was not received. Between our record and those maintained in the OR, for example, we can piece together the circumstances if need be. I hope that some of you will share the process you use, especially if your procedure might be more effective in maintaining the privacy of the patient. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Ph: (843) 792-6353 Fax: (843) 792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From dellav <@t> musc.edu Wed Mar 5 16:20:08 2008 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Wed Mar 5 16:19:35 2008 Subject: [Histonet] potential HIPPA issue, would like your opinion In-Reply-To: <46982.64490.qm@web65702.mail.ac4.yahoo.com> References: <46982.64490.qm@web65702.mail.ac4.yahoo.com> Message-ID: Hi Rene, Are you referring to the requisition or a different document? The log that we use is in addition to the requisition required for all specimens. When does your delivery person fill in the data on your form, when they pick up the specimen or when it arrives at pathology? thanks Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Ph: (843) 792-6353 Fax: (843) 792-8974 ________________________________ From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Wednesday, March 05, 2008 4:47 PM To: Della Speranza, Vinnie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] potential HIPPA issue, would like your opinion We used a SINGLE form per patient that was deposited in a box. The delivery person filled the data in the form and placed it in the box, inaccessible to the next courier. Ren? J. "Della Speranza, Vinnie" wrote: Couriers delivering surgical or biopsy samples to our laboratory are asked to log in each sample they are delivering in a written record contained on a clipboard at the pathology receiving window at our facility. This occurs before any pathology staff actually accession the specimen and allows for an independent record completed by the courier of exactly what was delivered. An individual has contacted our Compliance office out of concern that because the record includes the patient's name, as well as other info including specimen, date, name of courier, etc, this information would be seen by subsequent couriers logging into this record. The record I'm referring to has on occasion assisted us in investigating when a clinician is looking for a report and we discover that the specimen was not received. Between our record and those maintained in the OR, for example, we can piece together the circumstances if need be. I hope that some of you will share the process you use, especially if your procedure might be more effective in maintaining the privacy of the patient. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Ph: (843) 792-6353 Fax: (843) 792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Looking for last minute shopping deals? Find them fast with Yahoo! Search. From anh2006 <@t> med.cornell.edu Wed Mar 5 17:12:42 2008 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Wed Mar 5 17:12:51 2008 Subject: [Histonet] CD31 vs. Von Willebrand Factor Message-ID: <601eea3817ed7.47cee29a@med.cornell.edu> Are you staining mouse tissue or human tissue? If mouse tissue, the vWF antibody from DAKO as well as the goat polyclonal anti-VE-Cadherin Ab from R&D work very well. If human tissue, the anti-CD31 from DAKO, clone JC/70 is excellent. ----- Original Message ----- From: jstaruk Date: Wednesday, March 5, 2008 2:05 pm Subject: [Histonet] CD31 vs. Von Willebrand Factor > Hello all, > > I have a request for a CD31 looking for angiogenesis. I know this > is a very > problematic antibody when working with paraffin sections. Can the Van > Willebrand antibody be substituted to obtain the same results? > > Thank you > > Jim > > _____________________ > Jim Staruk > Mass Histology Service > www.masshistology.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From kcai <@t> prosci-inc.com Wed Mar 5 18:06:11 2008 From: kcai <@t> prosci-inc.com (karen Cai) Date: Wed Mar 5 17:56:24 2008 Subject: [Histonet] Paraformaldehyde fixation and membrane Antibody Message-ID: <000001c87f1d$e33d3e80$7d01a8c0@prosci.com> =20 =20 Hello All, I am just wondering whether it=92s OK to use 4% paraformaldehyde as fixation agent for cells when I try to detect the membrane protein. Which kind of fixation agent is the best? I know formaldehyde is a cell-permeable agent. So even I don=92t use the Triton X-100 to permeablize the cell, the reagent can still move readily into living cells and cause wrong staining. Am I right? =20 Thanks in advance, =20 Best Regards, Karen No virus found in this outgoing message. Checked by AVG Free Edition.=20 Version: 7.5.516 / Virus Database: 269.21.4/1313 - Release Date: 3/5/2008 9:50 AM =20 From marktarango <@t> gmail.com Wed Mar 5 18:50:58 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Mar 5 18:51:04 2008 Subject: [Histonet] Paraformaldehyde fixation and membrane Antibody In-Reply-To: <000001c87f1d$e33d3e80$7d01a8c0@prosci.com> References: <000001c87f1d$e33d3e80$7d01a8c0@prosci.com> Message-ID: <5b6eb13e0803051650n459d795bt2111202feedb4e30@mail.gmail.com> I'd worry about "wrong staining" when you see something that looks funny. Have you already tried staining? You might have the best luck purchasing a commercial reagent for this, like cytofix/cytoperm from BD. Lots of labs (both clinical and research) use this stuff. Determining the best fixative often depends on the antigen of interest. I'm assuming these cells are from culture... Are they suspension or are they adherent cells? If they are adherent cells, are you going to trypsanize and stain them in suspension? Are you using something like chamber slides? 2008/3/5 karen Cai kcai@prosci-inc.com: > > > Hello All, > I am just wondering whether it's OK to use 4% paraformaldehyde as > fixation agent for cells when I try to detect the membrane protein. > Which kind of fixation agent is the best? I know formaldehyde is a > cell-permeable agent. So even I don't use the Triton X-100 to > permeablize the cell, the reagent can still move readily into living > cells and cause wrong staining. Am I right? > > Thanks in advance, > > Best Regards, > Karen > > No virus found in this outgoing message. > Checked by AVG Free Edition. > Version: 7.5.516 / Virus Database: 269.21.4/1313 - Release Date: > 3/5/2008 9:50 AM > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From conniegrubaugh <@t> hotmail.com Wed Mar 5 22:19:16 2008 From: conniegrubaugh <@t> hotmail.com (connie grubaugh) Date: Wed Mar 5 22:19:22 2008 Subject: [Histonet] Nevada Societyof Histotechnolgy Message-ID: The NVSH meeting has been changed from March 29th to May 2 nd. The agenda is still being worked on but will still be held at Sunrise Hospital sky room. Connie GrubaughPast President of the Nevada Society of Histotechnolgy _________________________________________________________________ Connect and share in new ways with Windows Live. http://www.windowslive.com/share.html?ocid=TXT_TAGHM_Wave2_sharelife_012008 From ree3 <@t> leicester.ac.uk Thu Mar 6 03:17:12 2008 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Thu Mar 6 03:17:29 2008 Subject: [Histonet] H&E control slide In-Reply-To: <000001c87ee8$57a1e7c0$d00f7ca5@lurie.northwestern.edu> References: <47CE8895.2B7F.00C9.0@geisinger.edu> <000001c87ee8$57a1e7c0$d00f7ca5@lurie.northwestern.edu> Message-ID: <7722595275A4DD4FA225B92CDBF174A102BDD342@EXC-MBX3.cfs.le.ac.uk> Try tongue............. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: 05 March 2008 17:43 To: 'Angela Bitting'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] H&E control slide We use tonsil or other lymphoid tissue- if it does not stain as it should (where we can see the nucleus) we know somethings up. ernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Wednesday, March 05, 2008 10:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E control slide What is the consensus on what tissue to use for an H&E control. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> CDC.GOV Thu Mar 6 06:09:38 2008 From: jqb7 <@t> CDC.GOV (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu Mar 6 06:09:50 2008 Subject: [Histonet] Publications on block soaking Message-ID: <34BB307EFC9A65429BBB49E330675F72045E2691@LTA3VS003.ees.hhs.gov> Hi everyone, Does anyone know of any publications that show whether or not extensive soaking in ice water of faced, paraffin blocks will adversely affect staining whether by H&E, IHC, or special stains? Thanks, Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov From minhan.tan <@t> gmail.com Thu Mar 6 07:08:29 2008 From: minhan.tan <@t> gmail.com (Min-Han Tan) Date: Thu Mar 6 07:08:36 2008 Subject: [Histonet] Tris buffer In-Reply-To: References: Message-ID: <920cc4a70803060508l5c4e4702ic41be7ab1d90736d@mail.gmail.com> Dear Margaret, Analyzing the recipe you have provided : you are preparing a solution of pH 7.1, Tris-HCl 50 mM, with NaCl concentration of 150 mM. This is based on the Henderson Hasselbach equation = 8.3 + log (1.4/121)/ (6/157.6), where 8.3 is the pKa of Tris. The molarity of Tris is based on standard molecular weight calculations, Tris FW = 121 and Tris-HCl FW = 157.6. This seems like a reasonable recipe (NaCl = 150 mM - physiologic), except that perhaps I might aim for 7.4 or 7.2 from sheer familiarity. :) Regards, /min-han Min-Han Tan MBBS, MRCP(UK) Department of Medical Oncology National Cancer Centre Singapore On Wed, Mar 5, 2008 at 5:46 AM, Perry, Margaret wrote: > We need to update our SOP's and I can't find a reference for our tris > buffer recipe. I can find many recipes but not ours. Our recipe is as > follows: > > > > 6 grams Trizma Hydrochloride > > 1.4 grams Trizma base > > 8.75 grams Sodium chloride > > Qs with water to 1 liter > > > > Do any of you have a reference for this recipe? > > > > Margaret Perry HT (ASCP) > > IHC Lab Manager Veterinary Science > > Animal Disease Research and Diagnostic Lab > > South Dakota State University > > Box 2175 North Campus Drive > > Brookings SD 57007 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- /min-han From relia1 <@t> earthlink.net Thu Mar 6 07:35:11 2008 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Mar 6 07:35:21 2008 Subject: [Histonet] RELIA Histology Careers Bulletin 03/06/08 Are You in Your Dream Job? Message-ID: Hi Histonetters! What is your dream job? I am not talking about chief chocolate taster for Godiva or counting your lottery winnings. I mean are you in your dream job? If so what is it about your job that makes it great. If not what is it that would make your job the best? Is it more money? Is it more or less responsibility? Is it a different schedule? Is it a different location? If you are not in your dream job let me help!! Let's talk about it. Maybe I can find that dream job for you. For 25 years I have been helping people find their dream job! My services are free of charge to you and completely confidential. Shoot me an e-mail and tell me what it is that would make your dream job in histology. I have openings with my clients or we can customize a job search especially for you. All of the opportunities I represent are permanent positions with premier companies nationwide. They offer excellent salaries benefits and sign-on bonuses/relocation assistance. FYI here is a list of my newest and most current openings: My newest histology positions are located in: San Francisco, CA Shrevesport, LA St. Petersburg, FL Washington DC Lancaster, PA Pittsburgh, PA Harrisonburg, PA I also have openings in these areas: Austin, TX Corpus, Christi, TX Los Angeles, CA Aiken, SC Phoenix, AZ Orlando, FL Cincinatti, OH Helena, MT Concord, NH If you or any of your friends would like to chat about your dream job and how I can help or more information about any of the positions listed please contact me. I would be more than happy to assist you. You can reach me at 866-607-3542 or relia1@earthlink.net Remember it never hurts to look. Hope to hear from you soon . Thanks-Pam Thank You! Pam M. Barker President Relia 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll Free: (866)607-3542 e-mail: relia1@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia From rjbuesa <@t> yahoo.com Thu Mar 6 07:42:01 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 6 07:42:10 2008 Subject: [Histonet] potential HIPPA issue, would like your opinion In-Reply-To: Message-ID: <168307.67119.qm@web65702.mail.ac4.yahoo.com> Hi Vinnie: It was just a delivery form filled by the sending facility and left with the specimen at the lab along with the specimen. What the courier filled was just the ID numbers of the forms left at the lab, when they were delivered and by whom. Ren? J. "Della Speranza, Vinnie" wrote: v\:* {behavior:url(#default#VML);} o\:* {behavior:url(#default#VML);} w\:* {behavior:url(#default#VML);} .shape {behavior:url(#default#VML);} st1\:*{behavior:url(#default#ieooui) } Hi Rene, Are you referring to the requisition or a different document? The log that we use is in addition to the requisition required for all specimens. When does your delivery person fill in the data on your form, when they pick up the specimen or when it arrives at pathology? thanks Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Ph: (843) 792-6353 Fax: (843) 792-8974 --------------------------------- From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Wednesday, March 05, 2008 4:47 PM To: Della Speranza, Vinnie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] potential HIPPA issue, would like your opinion We used a SINGLE form per patient that was deposited in a box. The delivery person filled the data in the form and placed it in the box, inaccessible to the next courier. Ren? J. "Della Speranza, Vinnie" wrote: Couriers delivering surgical or biopsy samples to our laboratory are asked to log in each sample they are delivering in a written record contained on a clipboard at the pathology receiving window at our facility. This occurs before any pathology staff actually accession the specimen and allows for an independent record completed by the courier of exactly what was delivered. An individual has contacted our Compliance office out of concern that because the record includes the patient's name, as well as other info including specimen, date, name of courier, etc, this information would be seen by subsequent couriers logging into this record. The record I'm referring to has on occasion assisted us in investigating when a clinician is looking for a report and we discover that the specimen was not received. Between our record and those maintained in the OR, for example, we can piece together the circumstances if need be. I hope that some of you will share the process you use, especially if your procedure might be more effective in maintaining the privacy of the patient. Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Ph: (843) 792-6353 Fax: (843) 792-8974 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From b-frederick <@t> northwestern.edu Thu Mar 6 07:48:36 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Mar 6 07:48:49 2008 Subject: [Histonet] CD31 vs. Von Willebrand Factor In-Reply-To: <601eea3817ed7.47cee29a@med.cornell.edu> Message-ID: <001201c87f90$c9707df0$d00f7ca5@lurie.northwestern.edu> We use an anti-mouse cd31 for mouse tissue from what was Fitzgerald industries and I believe are now part of Invitrogen. For human I agree with you Andrea, we use the Dako CD31 for human, same with the VWF. It's polyclonal - don't know if we've used it on mouse. It does cross react with pig. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Hooper Sent: Wednesday, March 05, 2008 5:13 PM To: jstaruk Cc: 'histonet' Subject: Re: [Histonet] CD31 vs. Von Willebrand Factor Are you staining mouse tissue or human tissue? If mouse tissue, the vWF antibody from DAKO as well as the goat polyclonal anti-VE-Cadherin Ab from R&D work very well. If human tissue, the anti-CD31 from DAKO, clone JC/70 is excellent. ----- Original Message ----- From: jstaruk Date: Wednesday, March 5, 2008 2:05 pm Subject: [Histonet] CD31 vs. Von Willebrand Factor > Hello all, > > I have a request for a CD31 looking for angiogenesis. I know this > is a very > problematic antibody when working with paraffin sections. Can the Van > Willebrand antibody be substituted to obtain the same results? > > Thank you > > Jim > > _____________________ > Jim Staruk > Mass Histology Service > www.masshistology.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From minhan.tan <@t> gmail.com Thu Mar 6 07:57:24 2008 From: minhan.tan <@t> gmail.com (Min-Han Tan) Date: Thu Mar 6 07:57:49 2008 Subject: [Histonet] Question Message-ID: <920cc4a70803060557r5456026fq2f0574980b161d6b@mail.gmail.com> Dear Histonetters, Thanks in advance for your advice. I am interested in a possible red blood cell membrane protein, and am trying to stain it on a charged slide. The cells float off after paraformaldehyde 4% fixation, but they are fine after methanol fixation. Unfortunately, I do not see much staining of my protein of interest with the latter fixing method. Does anyone have any experience fixing RBC smears on slides so that the RBCs do not drift off? Thanks! -- /min-han From SharonC <@t> CarolinasPathology.com Thu Mar 6 08:13:40 2008 From: SharonC <@t> CarolinasPathology.com (Sharon Campbell) Date: Thu Mar 6 08:12:51 2008 Subject: [Histonet] Processing fatty breast tissue and fatty derm tissue Message-ID: Hello everyone, We are trying to improve on our processing of fat. We are currently using pen-fix fixation on our breast tissue. When we put it on the processor we are putting it through a formalin station and a pen-fix station. This works well most of the time. We may at times run large fatty derm tissues through along with the breasts. Is there a good way to process both at the same time? Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 (704) 549-8444 x100 sharonc@carolinaspathology.com From G.Fazzi <@t> FARMACO.unimaas.nl Thu Mar 6 08:42:04 2008 From: G.Fazzi <@t> FARMACO.unimaas.nl (Fazzi G (FARMACO)) Date: Thu Mar 6 08:43:26 2008 Subject: [Histonet] CD31 vs. Von Willebrand Factor Message-ID: <1C6586068DF1054DA3899159C38B32C909D2F7BE@um-mail0136.unimaas.nl> Hello all, What about capillary endothelium in rat muscle fixed in formalin and embedded in paraffin? What would be the best staining? I tried lectin GSL-B4 and CD31 but can't seem to get nice results. Gregorio -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Hooper Sent: donderdag 6 maart 2008 0:13 To: jstaruk Cc: 'histonet' Subject: Re: [Histonet] CD31 vs. Von Willebrand Factor Are you staining mouse tissue or human tissue? If mouse tissue, the vWF antibody from DAKO as well as the goat polyclonal anti-VE-Cadherin Ab from R&D work very well. If human tissue, the anti-CD31 from DAKO, clone JC/70 is excellent. ----- Original Message ----- From: jstaruk Date: Wednesday, March 5, 2008 2:05 pm Subject: [Histonet] CD31 vs. Von Willebrand Factor > Hello all, > > I have a request for a CD31 looking for angiogenesis. I know this > is a very > problematic antibody when working with paraffin sections. Can the Van > Willebrand antibody be substituted to obtain the same results? > > Thank you > > Jim > > _____________________ > Jim Staruk > Mass Histology Service > www.masshistology.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Thu Mar 6 08:59:28 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Mar 6 08:59:42 2008 Subject: [Histonet] Question In-Reply-To: <920cc4a70803060557r5456026fq2f0574980b161d6b@mail.gmail.com> References: <920cc4a70803060557r5456026fq2f0574980b161d6b@mail.gmail.com> Message-ID: <5b6eb13e0803060659m4418fdfcg80ada8a35f6d0867@mail.gmail.com> Have you tried just air-drying the slide? Either way, you need to make sure the slide is air-dried before fixing with anything. On Thu, Mar 6, 2008 at 5:57 AM, Min-Han Tan wrote: > Dear Histonetters, > > Thanks in advance for your advice. > > I am interested in a possible red blood cell membrane protein, and am > trying > to stain it on a charged slide. > > The cells float off after paraformaldehyde 4% fixation, but they are fine > after methanol fixation. > > Unfortunately, I do not see much staining of my protein of interest with > the > latter fixing method. > > Does anyone have any experience fixing RBC smears on slides so that the > RBCs > do not drift off? Thanks! > > -- > /min-han > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tissuearray <@t> hotmail.com Thu Mar 6 09:38:16 2008 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Thu Mar 6 09:38:24 2008 Subject: [Histonet] What product used for orientating tissue prior to embedding? Message-ID: Does anyone know what material or product is used to orientate tissues (skin and such) prior to processing? It's like a gel or something and it can be embedded with the tissues and it cuts nicely. Does anyone know what that stuff is? I think I have seen it in a pink color or probably blue... Thanks all, Thom _________________________________________________________________ Helping your favorite cause is as easy as instant messaging.?You IM, we give. http://im.live.com/Messenger/IM/Home/?source=text_hotmail_join From JWEEMS <@t> sjha.org Thu Mar 6 09:42:31 2008 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Thu Mar 6 09:43:18 2008 Subject: [Histonet] What product used for orientating tissue prior toembedding? In-Reply-To: References: Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD320518E8E7@sjhaexc02.sjha.org> It's Histogel - not sure your vendor, but you can google it..j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thom Jensen Sent: Thursday, March 06, 2008 10:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] What product used for orientating tissue prior toembedding? Does anyone know what material or product is used to orientate tissues (skin and such) prior to processing? It's like a gel or something and it can be embedded with the tissues and it cuts nicely. Does anyone know what that stuff is? I think I have seen it in a pink color or probably blue... Thanks all, Thom _________________________________________________________________ Helping your favorite cause is as easy as instant messaging.?You IM, we give. http://im.live.com/Messenger/IM/Home/?source=text_hotmail_join_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From trathborne <@t> somerset-healthcare.com Thu Mar 6 09:47:23 2008 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Mar 6 09:47:30 2008 Subject: [Histonet] What product used for orientating tissue prior to embedding? In-Reply-To: Message-ID: The product you are looking for is called Histogel. We purchase it from Richard Allan, catalog #HG-4000. We use a warm water bath to melt it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Thom Jensen Sent: Thursday, March 06, 2008 10:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] What product used for orientating tissue prior toembedding? Does anyone know what material or product is used to orientate tissues (skin and such) prior to processing? It's like a gel or something and it can be embedded with the tissues and it cuts nicely. Does anyone know what that stuff is? I think I have seen it in a pink color or probably blue... Thanks all, Thom _________________________________________________________________ Helping your favorite cause is as easy as instant messaging.?You IM, we give. http://im.live.com/Messenger/IM/Home/?source=text_hotmail_join_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. From jennifer.l.hofecker <@t> Vanderbilt.Edu Thu Mar 6 09:48:10 2008 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Thu Mar 6 09:48:20 2008 Subject: [Histonet] What product used for orientating tissue prior toembedding? In-Reply-To: Message-ID: <898D946569A27444B65667A49C0740520175AFDF@mailbe06.mc.vanderbilt.edu> Histogel All the way! Purchase Histogel from Thermo Scientific (formerly Richard Allan) There was a flurry of histogel activity on histonet a few months ago, you should be able to find info in the archives. Oh,and it is pink... Let me know if you need more specific info. Have a great rest of the week. Jennifer Jennifer L. Hofecker, HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph. (615)343-0083 fax. (615)343-7089 NSH Quality Control Committee Chair -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thom Jensen Sent: Thursday, March 06, 2008 9:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] What product used for orientating tissue prior toembedding? Does anyone know what material or product is used to orientate tissues (skin and such) prior to processing? It's like a gel or something and it can be embedded with the tissues and it cuts nicely. Does anyone know what that stuff is? I think I have seen it in a pink color or probably blue... Thanks all, Thom _________________________________________________________________ Helping your favorite cause is as easy as instant messaging.?You IM, we give. http://im.live.com/Messenger/IM/Home/?source=text_hotmail_join_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Mar 6 10:39:32 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 6 10:39:41 2008 Subject: [Histonet] What product used for orientating tissue prior to embedding? In-Reply-To: Message-ID: <495852.10232.qm@web65713.mail.ac4.yahoo.com> As an alternative I have used melted agar. Works very well and is cheaper. Ren? J. "Rathborne, Toni" wrote: The product you are looking for is called Histogel. We purchase it from Richard Allan, catalog #HG-4000. We use a warm water bath to melt it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Thom Jensen Sent: Thursday, March 06, 2008 10:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] What product used for orientating tissue prior toembedding? Does anyone know what material or product is used to orientate tissues (skin and such) prior to processing? It's like a gel or something and it can be embedded with the tissues and it cuts nicely. Does anyone know what that stuff is? I think I have seen it in a pink color or probably blue... Thanks all, Thom _________________________________________________________________ --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From ploykasek <@t> phenopath.com Thu Mar 6 10:39:36 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Thu Mar 6 10:39:55 2008 Subject: [Histonet] Antibody for CD7 clone LP15 Message-ID: Hi all. Is anyone using the antibody for CD7 clone LP15? I just found out that clone 272 has been discontinued, and the new item is clone LP15. A search on PubMed has turned up nothing thus far. The vendor says that LP15 is superior for detection in paraffin ( I wasn't having any trouble with 272!). At any rate, just shopping for new clones. Of course we'll have to get it in & re-validate it ( in our spare time!). As always, thanks for the help. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From Stacy_McLaughlin <@t> cooley-dickinson.org Thu Mar 6 10:47:48 2008 From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin) Date: Thu Mar 6 10:47:57 2008 Subject: [Histonet] SMMHC Message-ID: Hello Histonetters! My Pathologists want to bring on this new antibody: SMMHC (heavy chain smooth muscle myosin, a myoepithelial marker for breast cancer). We will be using a Ventana Benchmark. Does anyone know of any suppliers? If you do use this antibody, any feedback you'd be willing to share would be greatly appreciated! Sincerely, Stacy McLaughlin Cooley Dickinson Hospital From rjbuesa <@t> yahoo.com Thu Mar 6 10:55:42 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 6 10:55:51 2008 Subject: [Histonet] Processing fatty breast tissue and fatty derm tissue In-Reply-To: Message-ID: <185455.92658.qm@web65715.mail.ac4.yahoo.com> The water contents of fatty tissue is lower than that of other tissues, so dehydration should not be the issue, but you need to increase the time in the clearing (antemedium). I always used a station between the last alcohol and the first clearing station made up of 1:1 amounts of alcohol and clearing agent. And yes, you can run them together. My question is why you fix the tissue with both penfix and formaline? You should use only one of them. Ren? J. Sharon Campbell wrote: Hello everyone, We are trying to improve on our processing of fat. We are currently using pen-fix fixation on our breast tissue. When we put it on the processor we are putting it through a formalin station and a pen-fix station. This works well most of the time. We may at times run large fatty derm tissues through along with the breasts. Is there a good way to process both at the same time? Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 (704) 549-8444 x100 sharonc@carolinaspathology.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From kmerriam2003 <@t> yahoo.com Thu Mar 6 11:09:56 2008 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Thu Mar 6 11:10:04 2008 Subject: [Histonet] CD31 vs. Von Willebrand Factor In-Reply-To: Message-ID: <349254.24536.qm@web50305.mail.re2.yahoo.com> Hi Jim, We use Santa Cruz @ sc-28188 rabbit anti-PECAM (CD31) with Biocare's DIVA (EDTA) retrieval in B&D steamer for mouse FFPE tissue. Works like a charm every time. Kim Kim Merriam Cambridge, MA jstaruk wrote: Hello all, I have a request for a CD31 looking for angiogenesis. I know this is a very problematic antibody when working with paraffin sections. Can the Van Willebrand antibody be substituted to obtain the same results? Thank you Jim _____________________ Jim Staruk Mass Histology Service www.masshistology.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From drbugge <@t> gmail.com Thu Mar 6 11:34:46 2008 From: drbugge <@t> gmail.com (Dawn Bugge) Date: Thu Mar 6 11:34:53 2008 Subject: [Histonet] CAP Inspection deficiency GEN.66100 Emergency Power Message-ID: <1c4db3750803060934p761a24e9i886141c63c3bf8bc@mail.gmail.com> Our lab received a Phase I deficiency for not having emergency power back-up. I am not sure how to handle this issue besides a gas powered generator which isn't do-able with the set-up we have. I don't believe our building will allow this. Does anyone know of any emergency back-up generator that does not require gas, etc. -- Dawn R Bugge Seattle Histology From info <@t> hphisto.com Thu Mar 6 11:49:38 2008 From: info <@t> hphisto.com (info@hphisto.com) Date: Thu Mar 6 11:49:44 2008 Subject: [Histonet] Help...in need of TB (AFB) control tissue Message-ID: <32341243.346901204825778006.JavaMail.servlet@perfora> Is therre anyone out there willing to part with a sliver or two of TB/AFB control. It is needed at Avera-McKennan Histology lab in Sioux Falls, SD. Their regular source (Newcomer??) is out. -- Bill O'Donnell HT (ASCP) QIHC High Performance Histology Services LLC www.hphisto.com From gmartin <@t> marshallmedical.org Thu Mar 6 11:54:00 2008 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Thu Mar 6 11:54:15 2008 Subject: [Histonet] emergency backup Message-ID: <6ED9D4252F278841A0593D3D788AF24C01E203AE@mailsvr.MARSHMED.local> Are you sure they didn't mean emergency lighting backup? We got dinged for that and we simply installed battery powered emergency lights. Gary From crochieresteve <@t> aol.com Thu Mar 6 11:58:31 2008 From: crochieresteve <@t> aol.com (crochieresteve@aol.com) Date: Thu Mar 6 11:58:50 2008 Subject: [Histonet] VIP manual Message-ID: <8CA4DCAD309F80D-C24-1EBE@webmail-md14.sysops.aol.com> I have inherited a VIP 2000 for use in a satellite lab that I am in the middle of setting up. It did not come with a user manual and I haven't used this type of?processor for at least 10 years and can't remember how to get it running. Does anyone have a copy of the user manual or know where I can get one? I'd really appreciate any help. thanks Steve Crochiere, HT(ASCP) Histology manager NEPA, LifePath Partners, LLC. Mercy Medical Center From Ronald.Houston <@t> nationwidechildrens.org Thu Mar 6 12:05:03 2008 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Thu Mar 6 12:05:44 2008 Subject: [Histonet] SMMHC In-Reply-To: Message-ID: <979FF5962E234F45B06CF0DB7C1AABB215C858F9@chi2k3ms01.columbuschildrens.net> We use the myosin heavy chain, smooth muscle, clone S131, from Novocastra (Leica Microsystems) with great results, although our focus is not on breast cancer. Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 Ronald.Houston@NationwideChildrens.org Columbus Children's Hospital is now Nationwide Children's Hospital www.NationwideChildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stacy McLaughlin Sent: Thursday, March 06, 2008 11:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] SMMHC Hello Histonetters! My Pathologists want to bring on this new antibody: SMMHC (heavy chain smooth muscle myosin, a myoepithelial marker for breast cancer). We will be using a Ventana Benchmark. Does anyone know of any suppliers? If you do use this antibody, any feedback you'd be willing to share would be greatly appreciated! Sincerely, Stacy McLaughlin Cooley Dickinson Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From mona_diane <@t> hotmail.com Thu Mar 6 12:25:26 2008 From: mona_diane <@t> hotmail.com (Ramona Turner) Date: Thu Mar 6 12:25:40 2008 Subject: [Histonet] RE: Histonet Digest, Vol 52, Issue 9 In-Reply-To: References: Message-ID: I also would like some advice on processing fatty tissue. I have tried using Pen-Fix prior to processing, but my pathologist complain about an artifact and refuse to use it anymore. Can't explain that one. I am currently processing with formalin x2, 70% x2, 95% x2, 100% x2, clearing x2. I thought the dehydration step should be increased on the fatty tissue, but you are saying that is not the problem? Can someone else please offer some experience on this issue. Ramona Turner, HT (ASCP) Potomac Hospital Woodbridge, VA _________________________________________________________________ Shed those extra pounds with MSN and The Biggest Loser! http://biggestloser.msn.com/ From mona_diane <@t> hotmail.com Thu Mar 6 12:25:39 2008 From: mona_diane <@t> hotmail.com (Ramona Turner) Date: Thu Mar 6 12:25:42 2008 Subject: [Histonet] Re: Processing fatty breast tissue and fatty derm tissue In-Reply-To: References: Message-ID: I also would like some advice on processing fatty tissue. I have tried using Pen-Fix prior to processing, but my pathologist complain about an artifact and refuse to use it anymore. Can't explain that one. I am currently processing with formalin x2, 70% x2, 95% x2, 100% x2, clearing x2. I thought the dehydration step should be increased on the fatty tissue, but you are saying that is not the problem? Can someone else please offer some experience on this issue. Ramona Turner, HT (ASCP) Potomac Hospital Woodbridge, VA _________________________________________________________________ Climb to the top of the charts!?Play the word scramble challenge with star power. http://club.live.com/star_shuffle.aspx?icid=starshuffle_wlmailtextlink_jan From jstaruk <@t> masshistology.com Thu Mar 6 12:28:43 2008 From: jstaruk <@t> masshistology.com (jstaruk) Date: Thu Mar 6 12:28:52 2008 Subject: [Histonet] Help...in need of TB (AFB) control tissue In-Reply-To: <32341243.346901204825778006.JavaMail.servlet@perfora> Message-ID: <70C989C9375D489C8702E80A9024572E@JimPC> We just got a large ABF-positive tissue. Please send me your contact information. Jim _____________________ Jim Staruk Mass Histology Service www.masshistology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of info@hphisto.com Sent: Thursday, March 06, 2008 12:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help...in need of TB (AFB) control tissue Is therre anyone out there willing to part with a sliver or two of TB/AFB control. It is needed at Avera-McKennan Histology lab in Sioux Falls, SD. Their regular source (Newcomer??) is out. -- Bill O'Donnell HT (ASCP) QIHC High Performance Histology Services LLC www.hphisto.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Paul <@t> Firnschild.com Thu Mar 6 12:45:10 2008 From: Paul <@t> Firnschild.com (Paul Firnschild) Date: Thu Mar 6 12:45:20 2008 Subject: [Histonet] VIP manual References: <8CA4DCAD309F80D-C24-1EBE@webmail-md14.sysops.aol.com> Message-ID: <03de01c87fba$34e35f20$cf977e18@PhelpsDodge> I can send you a manual, Steve. No problem. Just email me your snail mail address and I'll pop one in the mail for you. Paul M. Firnschild QA Support Services, Inc. 404.291.3715 (t) 419.818.3618 (f) email: Paul@Firnschild.com ----- Original Message ----- From: To: Sent: Thursday, March 06, 2008 12:58 PM Subject: [Histonet] VIP manual > I have inherited a VIP 2000 for use in a satellite lab that I am in the middle of setting up. It did not come with a user manual and I haven't used this type of?processor for at least 10 years and can't remember how to get it running. Does anyone have a copy of the user manual or know where I can get one? I'd really appreciate any help. > thanks > Steve Crochiere, HT(ASCP) > Histology manager > NEPA, LifePath Partners, LLC. > Mercy Medical Center > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From SharonC <@t> CarolinasPathology.com Thu Mar 6 12:48:02 2008 From: SharonC <@t> CarolinasPathology.com (Sharon Campbell) Date: Thu Mar 6 12:47:30 2008 Subject: [Histonet] H&E Stainers Message-ID: Hello, We are getting ready to demo a number of H&E autostainers this year. I was wondering what recommendations anyone has for me. We want a stainer that can handle about 3-5 racks in the loading position. We tend to have about 4 racks backed up waiting to get on the stainer about mid morning. Sharon Joyce From SohrabB1 <@t> ah.org Thu Mar 6 10:44:38 2008 From: SohrabB1 <@t> ah.org (Behnaz Sohrab) Date: Thu Mar 6 12:47:41 2008 Subject: [Histonet] Fwd: Staining Problem References: <47CF9DD2.4347.0054.0@ah.org> Message-ID: <47CFAEF4.4347.0054.0@ah.org> >>> Behnaz Sohrab 3/6/2008 07:31 >>> Blue shift making nuclei over-stained and effecting mucous and stroma of tissues like umbilical cord, membranes. This also appears on our G.I. biopsies? Any one else has seen this ? It is not consistent either. Any suggestion would be appreciate. We do use Leica stainer with gill-III Behnaz Sohrab,Histo supervisor From doug <@t> ppspath.com Thu Mar 6 12:57:59 2008 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Thu Mar 6 12:59:20 2008 Subject: SPAM-LOW: [Histonet] Processing fatty breast tissue and fatty derm tissue In-Reply-To: Message-ID: Sharon, Are you running Her2 on those breast cases? If so then please read the ASCO/CAP Her2 guidelines. http://arpa.allenpress.com/pdf/i1543-2165-131-1-18.pdf Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon Campbell Sent: Thursday, March 06, 2008 9:14 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Processing fatty breast tissue and fatty derm tissue Hello everyone, We are trying to improve on our processing of fat. We are currently using pen-fix fixation on our breast tissue. When we put it on the processor we are putting it through a formalin station and a pen-fix station. This works well most of the time. We may at times run large fatty derm tissues through along with the breasts. Is there a good way to process both at the same time? Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 (704) 549-8444 x100 sharonc@carolinaspathology.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Thu Mar 6 13:36:57 2008 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Thu Mar 6 13:37:59 2008 Subject: SPAM-LOW: [Histonet] Re: Processing fatty breast tissue and fatty derm tissue In-Reply-To: Message-ID: Ramona, There are many reasons why facilities have problems with this. I believe that it all starts at the grossing bench. The specimen should be breadloafed when it arrives to maximize fixation. The tissue must also be sliced (for cassette) at a reasonable thickness. The next stumbling block is time. You can not rush these types of tissue. They must be fixed properly. 10% NBF is fine for these tissues. Let them sit overnight in the cassettes in the 10% NBF. Place them in a container on top of a stir-plate to maximize this. It also helps if you have an extra processor just for these types of tissue. Rene is also correct about the extended clearing time. I also like to extend the paraffin times to get maximum infiltration. This helps support the fat when cutting. Your chemicals could also be adjusted. The 70% x 2 is not necessary. I would remove one of those and add an extra clearing step or as Rene suggested the 1:1 mix. None of your stations should be less than an hour also. I know that this kills TAT but if your pathologist communicated with the surgeons and explained the importance of this then they should not mind. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ramona Turner Sent: Thursday, March 06, 2008 1:26 PM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Re: Processing fatty breast tissue and fatty derm tissue I also would like some advice on processing fatty tissue. I have tried using Pen-Fix prior to processing, but my pathologist complain about an artifact and refuse to use it anymore. Can't explain that one. I am currently processing with formalin x2, 70% x2, 95% x2, 100% x2, clearing x2. I thought the dehydration step should be increased on the fatty tissue, but you are saying that is not the problem? Can someone else please offer some experience on this issue. Ramona Turner, HT (ASCP) Potomac Hospital Woodbridge, VA _________________________________________________________________ Climb to the top of the charts!?Play the word scramble challenge with star power. http://club.live.com/star_shuffle.aspx?icid=starshuffle_wlmailtextlink_jan__ _____________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Thu Mar 6 13:43:04 2008 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Thu Mar 6 13:44:10 2008 Subject: SPAM-LOW: [Histonet] H&E Stainers In-Reply-To: Message-ID: Sharon, Check this one out. http://www.ventanamed.com/products/instruments/symphony_brochure.pdf Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon Campbell Sent: Thursday, March 06, 2008 1:48 PM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] H&E Stainers Hello, We are getting ready to demo a number of H&E autostainers this year. I was wondering what recommendations anyone has for me. We want a stainer that can handle about 3-5 racks in the loading position. We tend to have about 4 racks backed up waiting to get on the stainer about mid morning. Sharon Joyce _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Mandy.Bell <@t> chomp.org Thu Mar 6 13:52:51 2008 From: Mandy.Bell <@t> chomp.org (Bell, Mandy) Date: Thu Mar 6 13:53:21 2008 Subject: [Histonet] bodians stain Message-ID: Does anyone have any tips on getting the Bodian method stain to work? My coworker has been trying for the past few days, and the tissue is not turning dark tan to brown in the protargol solution. We've tried microwaving and letting it sit in the warm solution for 20 min, and repeating this multiple times, and just letting it sit in the protargol overnight. The only thing we've thought of is that the protargol solution may have been shaken up, which the procedure says not to do. Any tips would be greatly appreciated. (We're using FFPE brain tissue cut at 8 microns) Thanks. Mandy M Bell Histology Department Community Hospital of the Monterey Peninsula 831.625.4791 From rjbuesa <@t> yahoo.com Thu Mar 6 14:04:27 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 6 14:04:33 2008 Subject: [Histonet] CD31 vs. Von Willebrand Factor In-Reply-To: <1C6586068DF1054DA3899159C38B32C909D2F7BE@um-mail0136.unimaas.nl> Message-ID: <458320.41126.qm@web65705.mail.ac4.yahoo.com> Whenever I had a chance I run a comparative test with Ulex europaeus lectins with very good reaction results. Ren? J. "Fazzi G (FARMACO)" wrote: Hello all, What about capillary endothelium in rat muscle fixed in formalin and embedded in paraffin? What would be the best staining? I tried lectin GSL-B4 and CD31 but can't seem to get nice results. Gregorio -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Hooper Sent: donderdag 6 maart 2008 0:13 To: jstaruk Cc: 'histonet' Subject: Re: [Histonet] CD31 vs. Von Willebrand Factor Are you staining mouse tissue or human tissue? If mouse tissue, the vWF antibody from DAKO as well as the goat polyclonal anti-VE-Cadherin Ab from R&D work very well. If human tissue, the anti-CD31 from DAKO, clone JC/70 is excellent. ----- Original Message ----- From: jstaruk Date: Wednesday, March 5, 2008 2:05 pm Subject: [Histonet] CD31 vs. Von Willebrand Factor > Hello all, > > I have a request for a CD31 looking for angiogenesis. I know this > is a very > problematic antibody when working with paraffin sections. Can the Van > Willebrand antibody be substituted to obtain the same results? > > Thank you > > Jim > > _____________________ > Jim Staruk > Mass Histology Service > www.masshistology.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From llewllew <@t> shaw.ca Thu Mar 6 14:04:35 2008 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Thu Mar 6 14:04:58 2008 Subject: [Histonet] bodians stain References: Message-ID: <000f01c87fc5$4d4ac610$bd144246@yourlk4rlmsu> Protargol is a protein-silver complex and is a very variable material. We had the same problem many years ago. The resolution was to get several samples of protargol from different sources and choose one that worked. Also, make sure you use copper shot about 2 mm diameter. Bryan Llewellyn ----- Original Message ----- From: "Bell, Mandy" To: Sent: Thursday, March 06, 2008 11:52 AM Subject: [Histonet] bodians stain Does anyone have any tips on getting the Bodian method stain to work? My coworker has been trying for the past few days, and the tissue is not turning dark tan to brown in the protargol solution. We've tried microwaving and letting it sit in the warm solution for 20 min, and repeating this multiple times, and just letting it sit in the protargol overnight. The only thing we've thought of is that the protargol solution may have been shaken up, which the procedure says not to do. Any tips would be greatly appreciated. (We're using FFPE brain tissue cut at 8 microns) Thanks. Mandy M Bell Histology Department Community Hospital of the Monterey Peninsula 831.625.4791 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Mar 6 14:43:49 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 6 14:43:57 2008 Subject: [Histonet] bodians stain In-Reply-To: <000f01c87fc5$4d4ac610$bd144246@yourlk4rlmsu> Message-ID: <68113.12703.qm@web65714.mail.ac4.yahoo.com> Mandy: I used to prepare my own silver protargol fresh before each staining. I also developed a microwave procedure that could be completed in less than 2 hours. If you are interested I can send you a copy of my procedure. Ren? J. ----- Original Message ----- From: "Bell, Mandy" To: Sent: Thursday, March 06, 2008 11:52 AM Subject: [Histonet] bodians stain Does anyone have any tips on getting the Bodian method stain to work? My coworker has been trying for the past few days, and the tissue is not turning dark tan to brown in the protargol solution. We've tried microwaving and letting it sit in the warm solution for 20 min, and repeating this multiple times, and just letting it sit in the protargol overnight. The only thing we've thought of is that the protargol solution may have been shaken up, which the procedure says not to do. Any tips would be greatly appreciated. (We're using FFPE brain tissue cut at 8 microns) Thanks. Mandy M Bell Histology Department Community Hospital of the Monterey Peninsula 831.625.4791 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From trathborne <@t> somerset-healthcare.com Thu Mar 6 15:27:47 2008 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Mar 6 15:27:58 2008 Subject: [Histonet] Microwave Message-ID: We purchased a BP-125-IR microwave from Microwave Research Associates, Inc. It was purchased with the intent to replace the household microwave we were using for special stains. I'm hoping someone else has had success programming theirs for this purpose. The user's manual is not user friendly. Example: I'm looking for a way to bring Solution A from 5 to 60 C in as few steps as possible. With the old microwave, we would just set it for 50 seconds, and it would be done. Does anyone have experience with this microwave? Thanks in advance, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. From bakevictoria <@t> gmail.com Thu Mar 6 15:34:34 2008 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Thu Mar 6 15:34:43 2008 Subject: [Histonet] potential HIPPA issue, would like your opinion In-Reply-To: References: <005101c87ee7$04ad98d0$6901a8c0@FSROGER> Message-ID: <4f016b690803061334k72458940p905cfd3ddad5211b@mail.gmail.com> Vinnie, I've worked with these logs as well and I understand where Compliance is coming from and the way we had worked it was that:: - all specimens that are delivered to the lab have to be counter-signed by someone on the histology staff - this way we could be sure that we had everything and there were no reasons for us to reject the specimen for any reason - other than the patients name, no Unique Identifiers (SSN/Med.Rec#, DOB) are on the log. - log sheets are filed at the end of the day with any path req forms and kept for only 90 days and then destroyed in the 'confidential' disposal bin - ie. shredded If your lab staff has to do any pick ups at the OR or SDS or even the general refrigerator for weekend or after hours pick up they are also required to initial a log that usually has the patient "sticker" attached to the page with ALL the HIPPA regulated information included. The Compliance Department must have some sort of policy with them on how they handle this. All of these departments are considered controlled environments with access to these logs limited to only staff able to see any of this so there should be a universal hospital policy in place to cover this. Hope this helps. Vikki On 3/5/08, Della Speranza, Vinnie wrote: > Couriers delivering surgical or biopsy samples to our laboratory are asked to log in each sample they are delivering in a written record contained on a clipboard at the pathology receiving window at our facility. This occurs before any pathology staff actually accession the specimen and allows for an independent record completed by the courier of exactly what was delivered. > > An individual has contacted our Compliance office out of concern that because the record includes the patient's name, as well as other info including specimen, date, name of courier, etc, this information would be seen by subsequent couriers logging into this record. > > The record I'm referring to has on occasion assisted us in investigating when a clinician is looking for a report and we discover that the specimen was not received. Between our record and those maintained in the OR, for example, we can piece together the circumstances if need be. > > I hope that some of you will share the process you use, especially if your procedure might be more effective in maintaining the privacy of the patient. > > > Vinnie Della Speranza > Manager for Anatomic Pathology Services > Medical University of South Carolina > 165 Ashley Avenue Suite 309 > Charleston, South Carolina 29425 > Ph: (843) 792-6353 > Fax: (843) 792-8974 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From octavio109 <@t> hotmail.com Thu Mar 6 15:42:08 2008 From: octavio109 <@t> hotmail.com (Corinthia D. Emanuel) Date: Thu Mar 6 15:42:15 2008 Subject: [Histonet] (no subject) Message-ID: Nelson Dermatopathology is rapidly growing, extremely busy, and in immediate need of experienced techs! Nelson Dermatopathology, a physician-owned tissue pathology lab in Atlanta, GA, has an immediate need for a lab supervisor and experienced lab techs. Part-time and full-time positions are available and there are a variety of work shifts. Salary is based on experience. Please fax resume to 770-381-6451, attention C. Emanuel, for immediate consideration. _________________________________________________________________ Helping your favorite cause is as easy as instant messaging.?You IM, we give. http://im.live.com/Messenger/IM/Home/?source=text_hotmail_join From NMargaryan <@t> childrensmemorial.org Thu Mar 6 15:48:20 2008 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Thu Mar 6 15:47:29 2008 Subject: [Histonet] RE: Tissue Slide Storage - Antigen, DNA & RNA Preservation In-Reply-To: References: Message-ID: Dear Histonet: This is a very good topic to disscus. Dear Seniors, please give us a lesson: What is the best way to Store FFPE Tissue Slides to better Preserve Antigen, DNA & RNA? Thanks in advance, Naira -----Original Message----- Message: 2 Date: Wed, 5 Mar 2008 12:10:41 -0500 From: "McKnight, Tanisha" Subject: [Histonet] Tissue Slide Storage - Antigen, DNA & RNA Preservation To: histonet@lists.utsouthwestern.edu Message-ID: <816E3C72F855F14985FC31D7C963AE6F05051BFC@indexch03.ent.covance.com> Content-Type: text/plain; charset="us-ascii" Hello All: I am interested in exploring different methods of storing (long term) and shipping tissue slides for future IHC or Molecular Studies. So far, I've found that paraffin dipping and refrigeration is popular. But I've also heard of labs using nitrogen in ambient storage units in conjunction with paraffin dipping. Or some using nitrogen, refrigeration and paraffin dipping. The slides were then shipped in vacuum sealed bags. If anyone has experience with these methods, do you mind sharing them with me. Thanks. Tanisha N. McKnight, HT (ASCP) Specimen Management, Anatomic Pathology From nicholasprosenbaum <@t> yahoo.com Thu Mar 6 17:33:15 2008 From: nicholasprosenbaum <@t> yahoo.com (Nicholas Rosenbaum) Date: Thu Mar 6 17:33:20 2008 Subject: [Histonet] H&E control slide Message-ID: <476270.64906.qm@web32606.mail.mud.yahoo.com> We use uterus. Nicholas Rosenbaum, HT ASCP University of Virginia Medical Center ----- Original Message ---- From: Angela Bitting To: histonet@lists.utsouthwestern.edu Sent: Wednesday, March 5, 2008 11:48:37 AM Subject: [Histonet] H&E control slide What is the consensus on what tissue to use for an H&E control. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -----Inline Attachment Follows----- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Looking for last minute shopping deals? Find them fast with Yahoo! Search. http://tools.search.yahoo.com/newsearch/category.php?category=shopping From olek.michalski <@t> nencki.gov.pl Thu Mar 6 18:26:23 2008 From: olek.michalski <@t> nencki.gov.pl (Olek Michalski) Date: Thu Mar 6 18:19:46 2008 Subject: [Histonet] Vibratom operation - HELP Message-ID: Dear Histonetters, I am in major trouble. I am slicing Golgi-Cox impreganted brains into 120um sections. I used to embed tissue in 4% agar for slicing but it blocks cracked and the tissue was not cut very well. My coleague told me she prefer 30% gelatin for blocks, so I tried it and the sections are shattered. There are actually tiny stripes of tissue kept together by the gelatin block (or sometimes even not). I have to admit the blocks are really hard - they are composed of 30% w/w gelatin. The tissue was impreganted with Golgi-Cox solution (chromates and sublimate) for two weeks, then transferred to 30% sucrose for about a month then soaked with 10% gelatin (2 days in 37 deg.) and 30% gelatin (1 day about 50 deg.). I normally use 6% sucrose to fill the sectioning chamber. Could anybody help me, please? Yours sincerely Olek Michalski -- Laboratory of Neurobiology of Development and Evolution Nencki Institute of Experimental Biology ul. Pasteura 3, 02-093 Warszawa, Poland Tel. +48 22 5892268, Fax +48 22 8225342 From minhan.tan <@t> gmail.com Thu Mar 6 23:21:07 2008 From: minhan.tan <@t> gmail.com (Min-Han Tan) Date: Thu Mar 6 23:21:15 2008 Subject: [Histonet] Tris buffer In-Reply-To: <920cc4a70803060508l5c4e4702ic41be7ab1d90736d@mail.gmail.com> References: <920cc4a70803060508l5c4e4702ic41be7ab1d90736d@mail.gmail.com> Message-ID: <920cc4a70803062121t5ddd20b5tbf7c23d32e7a742@mail.gmail.com> Sorry, a final comment - the recipe may not be ideal because the buffering capacity of the TBST solution is quite low at this pH of 7.1. (i.e. the pH can swing quite a lot) The buffering capacity of a solution is usually best around pKa (in this case 8.3) +- 1 i.e. at a final pH of between 7.3 - 9.3. Regards, Min-han On Thu, Mar 6, 2008 at 9:08 PM, Min-Han Tan wrote: > Dear Margaret, > > Analyzing the recipe you have provided : you are preparing a solution of > pH 7.1, Tris-HCl 50 mM, with NaCl concentration of 150 mM. > > This is based on the Henderson Hasselbach equation = 8.3 + log (1.4/121)/ > (6/157.6), where 8.3 is the pKa of Tris. The molarity of Tris is based on > standard molecular weight calculations, Tris FW = 121 and Tris-HCl FW = > 157.6. > > This seems like a reasonable recipe (NaCl = 150 mM - physiologic), except > that perhaps I might aim for 7.4 or 7.2 from sheer familiarity. :) > > Regards, > > /min-han > > > Min-Han Tan > MBBS, MRCP(UK) > Department of Medical Oncology > National Cancer Centre Singapore > > > > > On Wed, Mar 5, 2008 at 5:46 AM, Perry, Margaret < > Margaret.Perry@sdstate.edu> wrote: > > > We need to update our SOP's and I can't find a reference for our tris > > buffer recipe. I can find many recipes but not ours. Our recipe is as > > follows: > > > > > > > > 6 grams Trizma Hydrochloride > > > > 1.4 grams Trizma base > > > > 8.75 grams Sodium chloride > > > > Qs with water to 1 liter > > > > > > > > Do any of you have a reference for this recipe? > > > > > > > > Margaret Perry HT (ASCP) > > > > IHC Lab Manager Veterinary Science > > > > Animal Disease Research and Diagnostic Lab > > > > South Dakota State University > > > > Box 2175 North Campus Drive > > > > Brookings SD 57007 > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > /min-han -- /min-han From Andrew.Prior <@t> Smith-Nephew.com Fri Mar 7 05:35:01 2008 From: Andrew.Prior <@t> Smith-Nephew.com (Prior, Andrew) Date: Fri Mar 7 05:35:16 2008 Subject: [Histonet] Alk Phos activity Message-ID: <6C18ADDF244BF8439412C063019CFFEC050E9A66@EHS021.wound.san> Dear Histonetters, I've had a request to do some enzyme histochemical staining to demonstrate Alkaline Phosphatase activity in bone (femoral condyles). >From reading through the literature it seems that this can only be done in unfixed frozen sections. However we need to do this in wax or MMA sections (due to the other stains being performed and to allow comparisons to previous studies). -Is it possible to demonstrate Alkaline Phosphatase activity in wax or resin sections? -From what I have read it seems FFPE sections are out - Is it the fixation or the processing that inactivates the enzyme? -Are there any fixatives that don't completely destroy the enzyme activity? I know frozen sections would work, but this is not an option. I look forward to hearing your suggestions. Thanks in advance Andrew Andrew Prior Histologist Smith &Nephew Research Centre York Science Park Heslington York YO10 5DF UK Andrew.Prior@smith-nephew.com 01904 824022 Confidentiality. This electronic transmission is strictly confidential to Smith & Nephew and intended solely for the addressee. It may contain information which is covered by legal, professional or other privilege. If you are not the intended addressee, or someone authorised by the intended addressee to receive transmissions on behalf of the addressee, you must not retain, disclose in any form, copy or take any action in reliance on this transmission. If you have received this transmission in error, please notify the sender as soon as possible and destroy this message. Smith & Nephew UK Limited Registered in England and Wales No.4421171 with registered office at 15 Adam Street, London WC2N 6LA From BMolinari <@t> heart.thi.tmc.edu Fri Mar 7 06:16:55 2008 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Mar 7 06:16:32 2008 Subject: [Histonet] VIP manual In-Reply-To: <8CA4DCAD309F80D-C24-1EBE@webmail-md14.sysops.aol.com> References: <8CA4DCAD309F80D-C24-1EBE@webmail-md14.sysops.aol.com> Message-ID: I have the manuals for the VIP 2000 (model 2000f 4618f) from 1991..still running great btw. Will those help? Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC 1-283 Houston, TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of crochieresteve@aol.com Sent: Thursday, March 06, 2008 11:59 AM To: histonet@pathology.swmed.edu Subject: [Histonet] VIP manual I have inherited a VIP 2000 for use in a satellite lab that I am in the middle of setting up. It did not come with a user manual and I haven't used this type of?processor for at least 10 years and can't remember how to get it running. Does anyone have a copy of the user manual or know where I can get one? I'd really appreciate any help. thanks Steve Crochiere, HT(ASCP) Histology manager NEPA, LifePath Partners, LLC. Mercy Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Mar 7 07:19:41 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 7 07:19:45 2008 Subject: [Histonet] CAP Inspection deficiency GEN.66100 Emergency Power In-Reply-To: <1c4db3750803060934p761a24e9i886141c63c3bf8bc@mail.gmail.com> Message-ID: <53999.93840.qm@web65709.mail.ac4.yahoo.com> That is something for your engineering department to take care of. They setup the system and you hook your instruments. The requirement is to have an emergency power supply. If your institution cannot provide it, then theirs should be the deficiency. Ren? J. Dawn Bugge wrote: Our lab received a Phase I deficiency for not having emergency power back-up. I am not sure how to handle this issue besides a gas powered generator which isn't do-able with the set-up we have. I don't believe our building will allow this. Does anyone know of any emergency back-up generator that does not require gas, etc. -- Dawn R Bugge Seattle Histology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From rjbuesa <@t> yahoo.com Fri Mar 7 07:22:47 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 7 07:22:52 2008 Subject: [Histonet] RE: Histonet Digest, Vol 52, Issue 9 In-Reply-To: Message-ID: <610279.98779.qm@web65715.mail.ac4.yahoo.com> The problem with fatty tissue is the fat, not the water. Fatty tissues require more clearing (fat dissolvents) and benefit also from prolonged processing protocols to allow the paraffin to infiltrate the areas where the fat has been removed. Ren? J Ramona Turner wrote: I also would like some advice on processing fatty tissue. I have tried using Pen-Fix prior to processing, but my pathologist complain about an artifact and refuse to use it anymore. Can't explain that one. I am currently processing with formalin x2, 70% x2, 95% x2, 100% x2, clearing x2. I thought the dehydration step should be increased on the fatty tissue, but you are saying that is not the problem? Can someone else please offer some experience on this issue. Ramona Turner, HT (ASCP) Potomac Hospital Woodbridge, VA _________________________________________________________________ Shed those extra pounds with MSN and The Biggest Loser! http://biggestloser.msn.com/_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From rjbuesa <@t> yahoo.com Fri Mar 7 07:23:45 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 7 07:23:53 2008 Subject: [Histonet] H&E Stainers In-Reply-To: Message-ID: <51787.48621.qm@web65716.mail.ac4.yahoo.com> Request a Sakura demo. You will buy it. Ren? J. Sharon Campbell wrote: Hello, We are getting ready to demo a number of H&E autostainers this year. I was wondering what recommendations anyone has for me. We want a stainer that can handle about 3-5 racks in the loading position. We tend to have about 4 racks backed up waiting to get on the stainer about mid morning. Sharon Joyce _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From yourbiomed <@t> cox.net Fri Mar 7 11:10:45 2008 From: yourbiomed <@t> cox.net (YourBiomed.Com ) Date: Fri Mar 7 11:10:50 2008 Subject: [Histonet] H&E Stainers In-Reply-To: <51787.48621.qm@web65716.mail.ac4.yahoo.com> Message-ID: <20080307121045.DADCL.93980.imail@fed1rmwml05> Do not get the Ventana H&E. It is broken more than it is running. We have a demo for the last 8 months and it's killing our turn around time. ---- Rene J Buesa wrote: Request a Sakura demo. You will buy it. Ren? J. Sharon Campbell wrote: Hello, We are getting ready to demo a number of H&E autostainers this year. I was wondering what recommendations anyone has for me. We want a stainer that can handle about 3-5 racks in the loading position. We tend to have about 4 racks backed up waiting to get on the stainer about mid morning. Sharon Joyce _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From yourbiomed <@t> cox.net Fri Mar 7 11:18:16 2008 From: yourbiomed <@t> cox.net (YourBiomed.Com ) Date: Fri Mar 7 11:18:25 2008 Subject: [Histonet] CAP Inspection deficiency GEN.66100 Emergency Power In-Reply-To: <53999.93840.qm@web65709.mail.ac4.yahoo.com> Message-ID: <20080307121816.WE934.94081.imail@fed1rmwml05> Are your critical instruments connected to battery UPS (Uninterruptible power supply)? This too is an option to meet the requirements. Not all labs have backup generators. ---- Rene J Buesa wrote: That is something for your engineering department to take care of. They setup the system and you hook your instruments. The requirement is to have an emergency power supply. If your institution cannot provide it, then theirs should be the deficiency. Ren? J. Dawn Bugge wrote: Our lab received a Phase I deficiency for not having emergency power back-up. I am not sure how to handle this issue besides a gas powered generator which isn't do-able with the set-up we have. I don't believe our building will allow this. Does anyone know of any emergency back-up generator that does not require gas, etc. -- Dawn R Bugge Seattle Histology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kappeler <@t> patho.unibe.ch Fri Mar 7 11:21:27 2008 From: kappeler <@t> patho.unibe.ch (Andi Kappeler) Date: Fri Mar 7 11:21:52 2008 Subject: AW: [Histonet] Antibody for CD7 clone LP15 In-Reply-To: References: Message-ID: <006201c88077$ad13d200$17955c82@pi23> Hi Patty we have compared clone LP15 with clone CD7-272 on various lymphoid tissues and bone marrow trephines. LP15 performed indeed better than CD7-272 (which I never really found that good). HIER is required for LP15, we had the best results with pressure cooker - citrate (better than microwave-citrate, -TE (pH 9.0), -Urea-Tris, pH 9.5)). Hope this helps. Best regards Andi Kappeler Institute of Pathology, University of Bern, Switzerland -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Patti Loykasek Gesendet: Donnerstag, 6. M?rz 2008 17:40 An: histonet Betreff: [Histonet] Antibody for CD7 clone LP15 Hi all. Is anyone using the antibody for CD7 clone LP15? I just found out that clone 272 has been discontinued, and the new item is clone LP15. A search on PubMed has turned up nothing thus far. The vendor says that LP15 is superior for detection in paraffin ( I wasn't having any trouble with 272!). At any rate, just shopping for new clones. Of course we'll have to get it in & re-validate it ( in our spare time!). As always, thanks for the help. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JCollins <@t> palmbeachpath.com Fri Mar 7 11:37:44 2008 From: JCollins <@t> palmbeachpath.com (Judy Collins) Date: Fri Mar 7 11:37:49 2008 Subject: [Histonet] SMMHC Message-ID: We use Ventana and recently set up the SMMHC in our lab. We purchased the antibody from Cell Marque, catalog #CMA569. It is just beautiful. Our pathologists love it. I will share our protocol for Ventana if you would like to E-mail me separately. Judy Collins Palm Beach Pathology E-mail: jcollins@palmbeachpath.com Hello Histonetters! My Pathologists want to bring on this new antibody: SMMHC (heavy chain smooth muscle myosin, a myoepithelial marker for breast cancer). We will be using a Ventana Benchmark. Does anyone know of any suppliers? If you do use this antibody, any feedback you'd be willing to share would be greatly appreciated! Sincerely, Stacy McLaughlin Cooley Dickinson Hospital From Robinsoc <@t> mercyhealth.com Fri Mar 7 12:34:31 2008 From: Robinsoc <@t> mercyhealth.com (Cynthia Robinson) Date: Fri Mar 7 12:34:59 2008 Subject: [Histonet] Melanin pigment Message-ID: <47D13657.59BC.00AF.0@mercyhealth.com> Has anyone successfully removed melanin pigment prior to IHC staining with MART-1? Currently we use Ventana I-View DAB and I have suggested switching to AEC for these cases but our paths don't want to spend the money for a second detection system. Any suggestions? Thanks......Cindi Cindi Robinson HT(ASCP) Mercy Medical Center-Sioux City Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 (712)279-2768 From doug <@t> ppspath.com Fri Mar 7 12:38:54 2008 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Fri Mar 7 12:40:07 2008 Subject: SPAM-LOW: Re: [Histonet] H&E Stainers In-Reply-To: <20080307121045.DADCL.93980.imail@fed1rmwml05> Message-ID: I call B.S. on this one. Are you an end user? Your email and website (YourBiomed.Com) says that you are a biomedical engineer? Please explain. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of YourBiomed.Com Sent: Friday, March 07, 2008 12:11 PM To: Rene J Buesa; Sharon Campbell; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: Re: [Histonet] H&E Stainers Do not get the Ventana H&E. It is broken more than it is running. We have a demo for the last 8 months and it's killing our turn around time. ---- Rene J Buesa wrote: Request a Sakura demo. You will buy it. Ren? J. Sharon Campbell wrote: Hello, We are getting ready to demo a number of H&E autostainers this year. I was wondering what recommendations anyone has for me. We want a stainer that can handle about 3-5 racks in the loading position. We tend to have about 4 racks backed up waiting to get on the stainer about mid morning. Sharon Joyce _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sjchtascp <@t> yahoo.com Fri Mar 7 12:40:34 2008 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Fri Mar 7 12:40:37 2008 Subject: [Histonet] looking for work Message-ID: <651360.2467.qm@web38204.mail.mud.yahoo.com> Anyone know of any "as needed" ht work in the Madison, Milwaukee, WI or No. Illinois area. 3-4 weeks anywhere in the US. --------------------------------- Never miss a thing. Make Yahoo your homepage. From marktarango <@t> gmail.com Fri Mar 7 13:13:49 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Mar 7 13:14:05 2008 Subject: SPAM-LOW: Re: [Histonet] H&E Stainers In-Reply-To: <47d18c33.2009360a.28b8.6cd0SMTPIN_ADDED@mx.google.com> References: <20080307121045.DADCL.93980.imail@fed1rmwml05> <47d18c33.2009360a.28b8.6cd0SMTPIN_ADDED@mx.google.com> Message-ID: <5b6eb13e0803071113g7d2d1578n14c926f4ed062aaa@mail.gmail.com> Good eye! On Fri, Mar 7, 2008 at 10:38 AM, Douglas D Deltour wrote: > I call B.S. on this one. Are you an end user? Your email and website ( > YourBiomed.Com) says that you are a biomedical engineer? Please explain. > > > > Douglas D. Deltour HT(ASCP) > Histology Manager > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > Office (803)252-1913 > Fax (803)254-3262 > Doug@ppspath.com > ***************************************************** > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of the individual or entity to > which it is addressed and may contain information that is privileged, > confidential and exempt from disclosure under applicable law. If the reader > of this message is not the intended recipient, you are hereby notified that > any dissemination, distribution, or copying of this communication is > strictly prohibited by law. If you have received this communication in > error, please notify me immediately. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of YourBiomed.Com > Sent: Friday, March 07, 2008 12:11 PM > To: Rene J Buesa; Sharon Campbell; histonet@lists.utsouthwestern.edu > Subject: SPAM-LOW: Re: [Histonet] H&E Stainers > > Do not get the Ventana H&E. It is broken more than it is running. > We have a demo for the last 8 months and it's killing our turn around > time. > > ---- Rene J Buesa wrote: > Request a Sakura demo. You will buy it. > Ren? J. > > Sharon Campbell wrote: > Hello, > > We are getting ready to demo a number of H&E autostainers this year. I > was wondering what recommendations anyone has for me. We want a stainer > that can handle about 3-5 racks in the loading position. We tend to have > about 4 racks backed up waiting to get on the stainer about mid morning. > > > > Sharon Joyce > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it > now. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From yourbiomed <@t> cox.net Fri Mar 7 14:01:10 2008 From: yourbiomed <@t> cox.net (YourBiomed.Com ) Date: Fri Mar 7 14:01:15 2008 Subject: SPAM-LOW: Re: [Histonet] H&E Stainers In-Reply-To: <5b6eb13e0803071113g7d2d1578n14c926f4ed062aaa@mail.gmail.com> Message-ID: <20080307150110.LO9KW.96021.imail@fed1rmwml05> (I consult on my free time) I work full time at a reference lab that has a Symphony(Demo) it has constantly gone down. Vacuum pumps (3 of them) vacuum tubing, leaking fittings. Coverslipper jams or applied unevenly onto slides. To put some merit on the issue. I worked at another reference lab before this one for 3+ years before being recruited to where I?m now. They too have a Symphony with the same issues. Both pressure, vacuum, leaking fittings, coverslipper cracking or misaligned on slides. The list above are repeated issues. There are many more but not as prevalent as what I listed. Please feel free to ask anyone who owns or is demoing this instrument to verify what I have stated. ---- Mark Tarango wrote: Good eye! On Fri, Mar 7, 2008 at 10:38 AM, Douglas D Deltour wrote: > I call B.S. on this one. Are you an end user? Your email and website ( > YourBiomed.Com) says that you are a biomedical engineer? Please explain. > > > > Douglas D. Deltour HT(ASCP) > Histology Manager > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > Office (803)252-1913 > Fax (803)254-3262 > Doug@ppspath.com > ***************************************************** > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of the individual or entity to > which it is addressed and may contain information that is privileged, > confidential and exempt from disclosure under applicable law. If the reader > of this message is not the intended recipient, you are hereby notified that > any dissemination, distribution, or copying of this communication is > strictly prohibited by law. If you have received this communication in > error, please notify me immediately. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of YourBiomed.Com > Sent: Friday, March 07, 2008 12:11 PM > To: Rene J Buesa; Sharon Campbell; histonet@lists.utsouthwestern.edu > Subject: SPAM-LOW: Re: [Histonet] H&E Stainers > > Do not get the Ventana H&E. It is broken more than it is running. > We have a demo for the last 8 months and it's killing our turn around > time. > > ---- Rene J Buesa wrote: > Request a Sakura demo. You will buy it. > Ren? J. > > Sharon Campbell wrote: > Hello, > > We are getting ready to demo a number of H&E autostainers this year. I > was wondering what recommendations anyone has for me. We want a stainer > that can handle about 3-5 racks in the loading position. We tend to have > about 4 racks backed up waiting to get on the stainer about mid morning. > > > > Sharon Joyce > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it > now. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From doug <@t> ppspath.com Fri Mar 7 14:15:26 2008 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Fri Mar 7 14:16:36 2008 Subject: SPAM-LOW: Re: [Histonet] H&E Stainers In-Reply-To: <20080307150110.LO9KW.96021.imail@fed1rmwml05> Message-ID: I see. Would you mind giving us the names of those labs for reference? Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of YourBiomed.Com Sent: Friday, March 07, 2008 3:01 PM To: Mark Tarango; Douglas D Deltour Cc: histonet@lists.utsouthwestern.edu Subject: Re: SPAM-LOW: Re: [Histonet] H&E Stainers (I consult on my free time) I work full time at a reference lab that has a Symphony(Demo) it has constantly gone down. Vacuum pumps (3 of them) vacuum tubing, leaking fittings. Coverslipper jams or applied unevenly onto slides. To put some merit on the issue. I worked at another reference lab before this one for 3+ years before being recruited to where I?m now. They too have a Symphony with the same issues. Both pressure, vacuum, leaking fittings, coverslipper cracking or misaligned on slides. The list above are repeated issues. There are many more but not as prevalent as what I listed. Please feel free to ask anyone who owns or is demoing this instrument to verify what I have stated. ---- Mark Tarango wrote: Good eye! On Fri, Mar 7, 2008 at 10:38 AM, Douglas D Deltour wrote: > I call B.S. on this one. Are you an end user? Your email and website ( > YourBiomed.Com) says that you are a biomedical engineer? Please explain. > > > > Douglas D. Deltour HT(ASCP) > Histology Manager > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > Office (803)252-1913 > Fax (803)254-3262 > Doug@ppspath.com > ***************************************************** > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of the individual or entity to > which it is addressed and may contain information that is privileged, > confidential and exempt from disclosure under applicable law. If the reader > of this message is not the intended recipient, you are hereby notified that > any dissemination, distribution, or copying of this communication is > strictly prohibited by law. If you have received this communication in > error, please notify me immediately. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of YourBiomed.Com > Sent: Friday, March 07, 2008 12:11 PM > To: Rene J Buesa; Sharon Campbell; histonet@lists.utsouthwestern.edu > Subject: SPAM-LOW: Re: [Histonet] H&E Stainers > > Do not get the Ventana H&E. It is broken more than it is running. > We have a demo for the last 8 months and it's killing our turn around > time. > > ---- Rene J Buesa wrote: > Request a Sakura demo. You will buy it. > Ren? J. > > Sharon Campbell wrote: > Hello, > > We are getting ready to demo a number of H&E autostainers this year. I > was wondering what recommendations anyone has for me. We want a stainer > that can handle about 3-5 racks in the loading position. We tend to have > about 4 racks backed up waiting to get on the stainer about mid morning. > > > > Sharon Joyce > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it > now. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Fri Mar 7 14:20:25 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Mar 7 14:20:34 2008 Subject: SPAM-LOW: Re: [Histonet] H&E Stainers In-Reply-To: <20080307150110.LO9KW.96021.imail@fed1rmwml05> References: <5b6eb13e0803071113g7d2d1578n14c926f4ed062aaa@mail.gmail.com> <20080307150110.LO9KW.96021.imail@fed1rmwml05> Message-ID: <5b6eb13e0803071220j200b8fa3peb474e901bda516d@mail.gmail.com> Wow. Incredible. On Fri, Mar 7, 2008 at 12:01 PM, YourBiomed.Com wrote: > (I consult on my free time) I work full time at a reference lab that has a > Symphony(Demo) it has constantly gone down. Vacuum pumps (3 of them) vacuum > tubing, leaking fittings. Coverslipper jams or applied unevenly onto slides. > To put some merit on the issue. I worked at another reference lab before > this one for 3+ years before being recruited to where I?m now. They too have > a Symphony with the same issues. Both pressure, vacuum, leaking fittings, > coverslipper cracking or misaligned on slides. The list above are repeated > issues. There are many more but not as prevalent as what I listed. Please > feel free to ask anyone who owns or is demoing this instrument to verify > what I have stated. > > > ---- Mark Tarango wrote: > Good eye! > > On Fri, Mar 7, 2008 at 10:38 AM, Douglas D Deltour > wrote: > > > I call B.S. on this one. Are you an end user? Your email and website ( > > YourBiomed.Com) says that you are a biomedical engineer? Please explain. > > > > > > > > Douglas D. Deltour HT(ASCP) > > Histology Manager > > Professional Pathology Services, PC > > One Science Court > > Suite 200 > > Columbia, SC 29203 > > Office (803)252-1913 > > Fax (803)254-3262 > > Doug@ppspath.com > > ***************************************************** > > PROFESSIONAL PATHOLOGY SERVICES, PC > > NOTICE OF CONFIDENTIALITY > > This message is intended only for the use of the individual or entity to > > which it is addressed and may contain information that is privileged, > > confidential and exempt from disclosure under applicable law. If the > reader > > of this message is not the intended recipient, you are hereby notified > that > > any dissemination, distribution, or copying of this communication is > > strictly prohibited by law. If you have received this communication in > > error, please notify me immediately. > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of YourBiomed.Com > > Sent: Friday, March 07, 2008 12:11 PM > > To: Rene J Buesa; Sharon Campbell; histonet@lists.utsouthwestern.edu > > Subject: SPAM-LOW: Re: [Histonet] H&E Stainers > > > > Do not get the Ventana H&E. It is broken more than it is running. > > We have a demo for the last 8 months and it's killing our turn around > > time. > > > > ---- Rene J Buesa wrote: > > Request a Sakura demo. You will buy it. > > Ren? J. > > > > Sharon Campbell wrote: > > Hello, > > > > We are getting ready to demo a number of H&E autostainers this year. I > > was wondering what recommendations anyone has for me. We want a stainer > > that can handle about 3-5 racks in the loading position. We tend to have > > about 4 racks backed up waiting to get on the stainer about mid morning. > > > > > > > > Sharon Joyce > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > --------------------------------- > > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try > it > > now. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From Sharon.Genest <@t> saskatoonhealthregion.ca Fri Mar 7 14:39:28 2008 From: Sharon.Genest <@t> saskatoonhealthregion.ca (Genest, Sharon SktnHR) Date: Fri Mar 7 14:40:21 2008 Subject: [Histonet] Slide Dryers Message-ID: <1C152FCB03A4F84197818DEE847F0D4A03B44E12@stampy.sktnhr.ca> Does anyone have a model of slide dryer that works well for them and a supplier name. We a presently using small individual dryers. I can't seem to find any of these and am unsure how people are tracking slides in and out of the larger dryers where multiple users would use the same dryer. Sharon Genest MLT Acting Technologist III Histology Saskatoon Health Region Phone: (306)655-8197 Email: sharon.genest@saskatoonhealthregion.ca From burch007 <@t> mc.duke.edu Fri Mar 7 14:42:47 2008 From: burch007 <@t> mc.duke.edu (James L Burchette) Date: Fri Mar 7 14:45:32 2008 Subject: [Histonet] Melanin pigment In-Reply-To: <47D13657.59BC.00AF.0@mercyhealth.com> Message-ID: Perform a Giemsa counter stain after the slides come off the stainer. The Giemsa will color the melanin pigment green. Jim Burchette, HT(ASCP) QIHC "A simple histotech from a little country hospital in North Carolina" "Cynthia Robinson" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/07/2008 01:34 PM To "histonet" cc Subject [Histonet] Melanin pigment Has anyone successfully removed melanin pigment prior to IHC staining with MART-1? Currently we use Ventana I-View DAB and I have suggested switching to AEC for these cases but our paths don't want to spend the money for a second detection system. Any suggestions? Thanks......Cindi Cindi Robinson HT(ASCP) Mercy Medical Center-Sioux City Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 (712)279-2768 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> Mercer.edu Fri Mar 7 15:30:48 2008 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Fri Mar 7 15:34:59 2008 Subject: [Histonet] Reminder for Region III Message-ID: <01MS5AE2O7BW0009OQ@Macon2.Mercer.edu> Hi Guys, Next Tuesday, March 11th, is the deadline for making your reservations for Region III meeting hosted by GSH. The Westin Peachtree Plaza has extended the deadline for registration for the Region III meeting in Atlanta Georgia April 3-5, 2008. Take advantage of the discounted rate of $129 single, double, triple or quad. The phone number to the Westin is 1-404-659-1400 and please state that you are attending the GSH/Region III meeting. The revised program can be downloaded from our website at www.histosearch.com/gsh. Click on the symposium link to get a PDF file of the program. Vendors have a link to their registration form to exhibit at the meeting on the same page. If you have questions Chris Coley, GSH Exhibit Liaison, has contact information is on that form There has been some confusion about the workshop fees. The $35 registration fee is nonrefundable and payable by everyone. This covers the seminars and Friday lunch, but the workshops, #1 through #5 are $40 each for members, $55 each for Nonmembers (either NSH or GSH) and $25 each for students (requires signature of program director). Workshops 1 and 2 will run concurrently and only one of them can be taken. Number 3 is Saturday morning, no conflicting workshop only seminars. Workshops 4 and 5 are in the afternoon running concurrently so only one of them can be taken. I hope this clears up any questions you may have. If anyone has further questions please feel to contact me at this email address. Come on down to Georgia, it is going to be beautiful. Shirley Powell GSH Secretary/Registrar From lmoak <@t> hardmanpath.com Fri Mar 7 15:45:08 2008 From: lmoak <@t> hardmanpath.com (Moak, Linda C.) Date: Fri Mar 7 15:43:32 2008 Subject: [Histonet] Available histotech position - Georgia Message-ID: Hardman Pathology and Dermatopathology (an Aurora Partner) located in Athens, Georgia, has a histology technician position available for an experienced tech. ASCP certification is required. Applicants must have knowledge/experience of grossing, processing and routine histology of skin specimens. Salary is based on experience. Excellent benefits are available. Qualified applicants, please fax resume to 706-546-4084 or email resume to lmoak@hardmanpath.com. From amosbrooks <@t> gmail.com Fri Mar 7 16:17:40 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri Mar 7 16:17:48 2008 Subject: [Histonet] H&E Stainers Message-ID: <582736990803071417y7c45bfb7ja9267f59e241fe57@mail.gmail.com> Charon, We have the Sakura DRS 2000 and I really like it. It can also run other programs as well if needed, such as PAS & Trichrome. Good shopping to you, Amos Message: 6 Date: Thu, 6 Mar 2008 13:48:02 -0500 From: "Sharon Campbell" Subject: [Histonet] H&E Stainers To: Message-ID: < AA9909F69C47934FA5A393874911121C8211E4@fs01sbs.Corp.CarolinasPathology.com> Content-Type: text/plain; charset="us-ascii" Hello, We are getting ready to demo a number of H&E autostainers this year. I was wondering what recommendations anyone has for me. We want a stainer that can handle about 3-5 racks in the loading position. We tend to have about 4 racks backed up waiting to get on the stainer about mid morning. Sharon Joyce From JWEEMS <@t> sjha.org Fri Mar 7 16:27:35 2008 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Mar 7 16:28:06 2008 Subject: [Histonet] H&E Stainers In-Reply-To: <582736990803071417y7c45bfb7ja9267f59e241fe57@mail.gmail.com> References: <582736990803071417y7c45bfb7ja9267f59e241fe57@mail.gmail.com> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD320518E93C@sjhaexc02.sjha.org> And we have the Leica.. It is a workhorse and does a great job. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Friday, March 07, 2008 5:18 PM To: SharonC@CarolinasPathology.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E Stainers Charon, We have the Sakura DRS 2000 and I really like it. It can also run other programs as well if needed, such as PAS & Trichrome. Good shopping to you, Amos Message: 6 Date: Thu, 6 Mar 2008 13:48:02 -0500 From: "Sharon Campbell" Subject: [Histonet] H&E Stainers To: Message-ID: < AA9909F69C47934FA5A393874911121C8211E4@fs01sbs.Corp.CarolinasPathology.c om> Content-Type: text/plain; charset="us-ascii" Hello, We are getting ready to demo a number of H&E autostainers this year. I was wondering what recommendations anyone has for me. We want a stainer that can handle about 3-5 racks in the loading position. We tend to have about 4 racks backed up waiting to get on the stainer about mid morning. Sharon Joyce _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From akbitting <@t> geisinger.edu Fri Mar 7 16:54:25 2008 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Mar 7 16:54:49 2008 Subject: [Histonet] PT testing materials Message-ID: <47D18151.2B7F.00C9.0@geisinger.edu> Does anyone know what the CAP requirements are for retention of proficiency testing materials? I couldn't find anything on their website. We have the IHC survey slides from 10 years back and I'd love to be able to pitch them. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From epeters <@t> zosanopharma.com Fri Mar 7 17:20:14 2008 From: epeters <@t> zosanopharma.com (Elaine Peters) Date: Fri Mar 7 17:18:57 2008 Subject: [Histonet] Leica Cryostat Manual Message-ID: Does anyone have a copy of the instruction manual for a Leica CM1800 cryostat? Thanks, Elaine Elaine Peters, PhD Zosano Pharma, Inc 34790 Ardentech Court Fremont, CA 94555 From gu.lang <@t> gmx.at Sat Mar 8 05:12:39 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Mar 8 05:12:48 2008 Subject: AW: [Histonet] Slide Dryers In-Reply-To: <1C152FCB03A4F84197818DEE847F0D4A03B44E12@stampy.sktnhr.ca> Message-ID: <000001c8810d$52d26fe0$eeeea8c0@dielangs.at> Sharon, we used to dry our slides in a "regular" incubator or oven with air circulation. We had six "places" to put the slide-racks. Three on the lower and three on the upper "floor" in the oven. We also had six clocks standing beside in two rows. And each place of the clock referred to a place in the oven. The laboratory-aid had to register, what clock had rung and put the rack out of the oven for deparaffination. - a continious flow untill we were ready with cutting. Now we have the Leica-Stainer with oven and the lab-aids can turn to other duties. The air-circulation in the dryer is an important feature. Without the drying-time was 35-45 min 60?C, with circulation is was 25 min and the slides stick very well on the glass. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Genest, Sharon SktnHR Gesendet: Freitag, 07. M?rz 2008 21:39 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Slide Dryers Does anyone have a model of slide dryer that works well for them and a supplier name. We a presently using small individual dryers. I can't seem to find any of these and am unsure how people are tracking slides in and out of the larger dryers where multiple users would use the same dryer. Sharon Genest MLT Acting Technologist III Histology Saskatoon Health Region Phone: (306)655-8197 Email: sharon.genest@saskatoonhealthregion.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From warleygomessantos <@t> hotmail.com Sat Mar 8 07:15:16 2008 From: warleygomessantos <@t> hotmail.com (warley gomes) Date: Sat Mar 8 07:15:23 2008 Subject: [Histonet] Best's carmin and blue nile stain Message-ID: I need to use the Best's carmin stain, to show gligogen in hepatocity, and Nile blue sulphate staining method for lipids in tissue include in parafine but I don't know the protocol to stainig. thanks, Warley. Brazil. _________________________________________________________________ Conhe?a o Windows Live Spaces, a rede de relacionamentos do Messenger! http://www.amigosdomessenger.com.br/ From rjbuesa <@t> yahoo.com Sat Mar 8 10:02:31 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Mar 8 10:02:36 2008 Subject: [Histonet] PT testing materials In-Reply-To: <47D18151.2B7F.00C9.0@geisinger.edu> Message-ID: <710350.79139.qm@web65709.mail.ac4.yahoo.com> Angela: You are not required to keep them. Remember that you stain them and your pathologists send their interpretation. Later, when you receive the results from the survey that implies that you made the test, and that is all. No laboratory is required to participate in the proficiency test, so CAP cannot require to keep something that you don't have if not participating. On the other hand, they are a very good material for residents' training and in that sense you should consult with your laboratory director before pitching them. Ren? J. Angela Bitting wrote: Does anyone know what the CAP requirements are for retention of proficiency testing materials? I couldn't find anything on their website. We have the IHC survey slides from 10 years back and I'd love to be able to pitch them. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From rjbuesa <@t> yahoo.com Sat Mar 8 10:22:11 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Mar 8 10:22:14 2008 Subject: [Histonet] Melanin pigment In-Reply-To: <47D13657.59BC.00AF.0@mercyhealth.com> Message-ID: <697056.92624.qm@web65709.mail.ac4.yahoo.com> Cynthia: The method is simple, BUT you have to be sure that the sections are completely adhered to the slides. Treat them with a 0.1% aq. sol. of potassium permanganate. Check under the microscope to see the melanin changed color. Wash with abundant dist. water Treat the sections with 0.3% aq. oxalic acid until there is no more color in the section. Wash and procede with yout IHC protocol. Ren? J. Cynthia Robinson wrote: Has anyone successfully removed melanin pigment prior to IHC staining with MART-1? Currently we use Ventana I-View DAB and I have suggested switching to AEC for these cases but our paths don't want to spend the money for a second detection system. Any suggestions? Thanks......Cindi Cindi Robinson HT(ASCP) Mercy Medical Center-Sioux City Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 (712)279-2768 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From hodges420 <@t> msn.com Sat Mar 8 11:29:53 2008 From: hodges420 <@t> msn.com (MARY T HODGES) Date: Sat Mar 8 11:29:57 2008 Subject: [Histonet] Melanin pigment In-Reply-To: <697056.92624.qm@web65709.mail.ac4.yahoo.com> References: <47D13657.59BC.00AF.0@mercyhealth.com> <697056.92624.qm@web65709.mail.ac4.yahoo.com> Message-ID: Use ALk Phos red instead of DAB for the staining , use of any acid attacks the quality of staining, melanin is to hard to remove from any specimen Tere Hodges> Date: Sat, 8 Mar 2008 08:22:11 -0800> From: rjbuesa@yahoo.com> To: Robinsoc@mercyhealth.com; histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Melanin pigment> CC: > > Cynthia:> The method is simple, BUT you have to be sure that the sections are completely adhered to the slides.> Treat them with a 0.1% aq. sol. of potassium permanganate. Check under the microscope to see the melanin changed color.> Wash with abundant dist. water> Treat the sections with 0.3% aq. oxalic acid until there is no more color in the section.> Wash and procede with yout IHC protocol.> Ren? J.> > Cynthia Robinson wrote:> Has anyone successfully removed melanin pigment prior to IHC staining with MART-1? Currently we use Ventana I-View DAB and I have suggested switching to AEC for these cases but our paths don't want to spend the money for a second detection system. Any suggestions?> > Thanks......Cindi> > Cindi Robinson HT(ASCP)> Mercy Medical Center-Sioux City> Dunes Medical Laboratories> 350 W Anchor Dr> Dakota Dunes SD 57049> (712)279-2768> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > ---------------------------------> Looking for last minute shopping deals? Find them fast with Yahoo! Search.> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Helping your favorite cause is as easy as instant messaging.?You IM, we give. http://im.live.com/Messenger/IM/Home/?source=text_hotmail_join From kemlo <@t> f2s.com Sat Mar 8 15:01:47 2008 From: kemlo <@t> f2s.com (kemlo) Date: Sat Mar 8 15:03:07 2008 Subject: [Histonet] Washing out formalin fixation (Lengthy) In-Reply-To: <5C0BED61F529364E86309CADEA63FEF20163F358@TRFT-EX01.xRothGen.nhs.uk> References: <5C0BED61F529364E86309CADEA63FEF20163F358@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <77912386698B4D52ABE3D63150C8CAB5@KemloPC> Oddly Terry, the primers in Histology Fixation supports you; but why shouldn't they? Time is a constant? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: 04 March 2008 17:35 To: Morken, Tim; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Washing out formalin fixation (Lengthy) "These studies these show that fixation of peptide spots (essentially zero thickness) attached to glass slides take over 6 hours to "fix" at room temperature. In this case they defined "fixed" as the point at which an antibody would no longer detect it's target epitope, or the reaction was severely diminished. They also showed that HIER reverses the "fixation" So far so good, and supports what I have been saying in this forum for years about the (long) time it takes to formalin fix tissue, and the misbelief that wetting them for a few hours is fixing. But then...... "This calls into question any method that relies on less than six hours formalin fixation at room temperature (ie, biopsies, just because they are small) and also the effect of exposing those short-fixed tissues to long exposure to 70% alcohol, or other aqueous solution, before clearing and embedding." I know not what this all means/is getting at. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: 04 March 2008 17:17 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Washing out formalin fixation (Lengthy) Thanks for the synopsis Bryan. I'd like to point out that the 2004 paper by Sompuram,and a followup in 2006 are very interesting. These studies these show that fixation of peptide spots (essentially zero thickness) attached to glass slides take over 6 hours to "fix" at room temperature. In this case they defined "fixed" as the point at which an antibody would no longer detect it's target epitope, or the reaction was severly diminished. They also showed that HIER reverses the "fixation." The 2006 paper also investigates why some epitopes are affected by formalin and others are not. Sompuram SR, Vani K, Messana E and Bogen SA. A molecular mechanism of formalin fixation and antigen retrieval. Am J Clin Pathol (2004);121: 190-199 Sompuram, et. al, A molecular model of antigen retrieval using a peptide array, Am J Clin Path 2006;125:91-98 Tim Morken Technical Support Manager Lab Vision Products Anatomical Pathology ThermoFisher Scientific -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan Hewlett Sent: Tuesday, March 04, 2008 7:56 AM To: Johnson, Teri; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Washing out formalin fixation (Lengthy) Hi Teri and everyone else on this thread, Washing out many of the effects of formaldehyde fixation on tissues with running water has been known for years (much longer than this old boy has been around). In modern terms, it is the essential underlying mechanism for so-called antigen retrieval (HIER). Formaldehyde fixes proteins by addition, with the formation of hydroxymethyl adducts on the reactive side chains of proteins. Once enough of these hydroxymethyl adducts are formed, and IF they are in close approximation to each other, they may slowly cross-link by the formation of methylene bridges. However, these adducts and initial cross-links are unstable and readily reversed by water and alcohol (see Kiernan (1999). It takes 24 hours at room temperature for all the hydroxymethyl adducts to form, i.e. maximal binding threshold (see Fox et al, 1985). If the tissue is then exposed to running water before all the adducts have formed (i.e. less than 24 hours), the reversal is very rapid. The shorter the time in formaldehyde, the more rapid the reversal. Even after the essential 24 hours fixation and also after a more lengthy 6 days fixation, running water will still remove the adducts and hydrolyse the methylene bridges. There is at least one publication (Helander, 1994) that provides data regarding this effect. After 24 hours fixation, 50% reversal occurred in less than 24 hours, 90% reversal was obtained after 6 days washing and for 6 days fixation 90% reversal after 4 weeks washing. It should be noted that these reversal times were obtained at ambient temperatures and the times may be considerably reduced by elevated temperatures. This reversal effect is also obtained on tissue sections that have been processed to wax. However, because of the additional shrinkage and hydrophobicity of the processed proteins, the reversal is slowed somewhat until the proteins re-hydrate. The reversal effect can also be aided by the presence of other ions in the water (the purpose of HIER buffers). Back in the sixties, in order to successfully demonstrate Ig's by IF, we were reversing the fixation effects on paraffin sections by placing them in hypotonic buffers for 2 days at 37C. Today, since we are all in a great rush for results, we obviously drive the reversal at higher temperatures to speed things up!! References: Baker JR (1958), Principles of Biological Microtechnique, Methuen & Co. Ltd. Hopwood D. Fixatives and fixation: A review. Histochemical journal (1969); 1, p19-55 Burnett MG. The mechanism of the formaldehyde clock reaction: Methylene glycol dehydration. J Chem. educ. (1982); 59, 160 Fox CH et al. Formaldehyde fixation. J Histochem Cytochem (1985); 33, 845-853 Helander KG. Kinetic studies of formaldehyde binding in tissue. Biotechnique and Histochemistry. (1994); 69, 177-179 Kiernan J.A., Histological and Histochemical Methods: Theory and Practice, 3rd Edition, (1999). Oxford: Butterworth-Heinemann. ISBN # 0-7506-3106-6. Shi SR, Gu J. and Taylor CR. Antigen Retrieval Techniques: Immunohistochemistry and Molecular Morphology. (2000) Eaton Publishing. ISBN 1-881299-43-0. Sompuram SR, Vani K, Messana E and Bogen SA. A molecular mechanism of formalin fixation and antigen retrieval. Am J Clin Pathol (2004);121: 190-199 Best regards, Bryan ----- Original Message ----- From: "Johnson, Teri" To: Sent: Monday, March 03, 2008 2:33 PM Subject: [Histonet] Washing out formalin fixation Last week, a researcher here asked me what the chemical mechanism was of washing out the effects of formalin fixation on the tissues with running water. In other words, how does it work? Anybody here know? Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ###################################################################### This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. ###################################################################### _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Sat Mar 8 18:00:32 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Sat Mar 8 18:00:42 2008 Subject: [Histonet] Washing out formalin fixation (Lengthy) In-Reply-To: <000d01c87e10$49506080$6500a8c0@mainbox> References: <000d01c87e10$49506080$6500a8c0@mainbox> Message-ID: <5b6eb13e0803081600q7561e64csb48bc2453d0a6cee@mail.gmail.com> I just want to point out again that this works poorly and wastes water... I don't want anyone to get too excited and ditch the HIER buffers. Nobody does this in the real world. It all goes back to what Ren? said, "It (pretty much) doesn't work that way." On Tue, Mar 4, 2008 at 7:56 AM, Bryan Hewlett wrote: > Hi Teri and everyone else on this thread, > > Washing out many of the effects of formaldehyde fixation on tissues with > running water has been known for years (much longer than this old boy has > been around). > In modern terms, it is the essential underlying mechanism for so-called > antigen retrieval (HIER). > > Formaldehyde fixes proteins by addition, with the formation of > hydroxymethyl > adducts on the reactive side chains of proteins. > Once enough of these hydroxymethyl adducts are formed, and IF they are in > close approximation to each other, they may slowly cross-link by the > formation of methylene bridges. > However, these adducts and initial cross-links are unstable and readily > reversed by water and alcohol (see Kiernan (1999). > It takes 24 hours at room temperature for all the hydroxymethyl adducts to > form, i.e. maximal binding threshold (see Fox et al, 1985). > If the tissue is then exposed to running water before all the adducts have > formed (i.e. less than 24 hours), the reversal is very rapid. > The shorter the time in formaldehyde, the more rapid the reversal. > Even after the essential 24 hours fixation and also after a more lengthy 6 > days fixation, running water will still remove the adducts and hydrolyse > the > methylene bridges. > There is at least one publication (Helander, 1994) that provides data > regarding this effect. > After 24 hours fixation, 50% reversal occurred in less than 24 hours, 90% > reversal was obtained after 6 days washing and for 6 days fixation 90% > reversal after 4 weeks washing. > It should be noted that these reversal times were obtained at ambient > temperatures and the times may be considerably reduced by elevated > temperatures. > > This reversal effect is also obtained on tissue sections that have been > processed to wax. > However, because of the additional shrinkage and hydrophobicity of the > processed proteins, the reversal is slowed somewhat until the proteins > re-hydrate. > The reversal effect can also be aided by the presence of other ions in the > water (the purpose of HIER buffers). > Back in the sixties, in order to successfully demonstrate Ig's by IF, we > were reversing the fixation effects on paraffin sections by placing them > in > hypotonic buffers for 2 days at 37C. > Today, since we are all in a great rush for results, we obviously drive > the > reversal at higher temperatures to speed things up!! > > References: > > Baker JR (1958), Principles of Biological Microtechnique, Methuen & Co. > Ltd. > Hopwood D. Fixatives and fixation: A review. Histochemical journal (1969); > 1, p19-55 > Burnett MG. The mechanism of the formaldehyde clock reaction: Methylene > glycol dehydration. J Chem. educ. (1982); 59, 160 > Fox CH et al. Formaldehyde fixation. J Histochem Cytochem (1985); 33, > 845-853 > Helander KG. Kinetic studies of formaldehyde binding in tissue. > Biotechnique > and Histochemistry. (1994); 69, 177-179 > Kiernan J.A., Histological and Histochemical Methods: Theory and Practice, > 3rd Edition, (1999). Oxford: Butterworth-Heinemann. ISBN # 0-7506-3106-6. > Shi SR, Gu J. and Taylor CR. Antigen Retrieval Techniques: > Immunohistochemistry and Molecular Morphology. (2000) Eaton Publishing. > ISBN > 1-881299-43-0. > Sompuram SR, Vani K, Messana E and Bogen SA. A molecular mechanism of > formalin fixation and antigen retrieval. Am J Clin Pathol (2004);121: > 190-199 > > > Best regards, > > Bryan > > ----- Original Message ----- > From: "Johnson, Teri" > To: > Sent: Monday, March 03, 2008 2:33 PM > Subject: [Histonet] Washing out formalin fixation > > > Last week, a researcher here asked me what the chemical mechanism was of > washing out the effects of formalin fixation on the tissues with running > water. In other words, how does it work? Anybody here know? > > Teri Johnson, HT(ASCP)QIHC > Managing Director Histology Facility > Stowers Institute for Medical Research > 1000 E. 50th St. > Kansas City, MO 64110 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From raj <@t> bluemarble.net Sat Mar 8 19:31:49 2008 From: raj <@t> bluemarble.net (Rebecca Johnson) Date: Sat Mar 8 19:32:10 2008 Subject: [Histonet] The Leica Polaris Message-ID: <00fc01c88185$58e18fb0$7b48f9d8@CHURCH> Has anyone had problems with loading two runs at one time? Also water contamination problems? From laurie <@t> conxis.com Sat Mar 8 21:40:06 2008 From: laurie <@t> conxis.com (Laurie Popp) Date: Sat Mar 8 21:40:15 2008 Subject: [Histonet] Re: H&E Stainers Message-ID: <47D35C16.3060003@conxis.com> actually we are "test driving" as you will the new Ventana Symphony stainer and have not had a day where we have not gotten a call from Tuscon stating that there is a problem with the stainer since it passed validation. The nice thing is tech support usually calls us right as the computer is starting to error. The bad thing is you have to go back and start your H&E's over from the beginning... destaining and restaining since there is no resume as of yet. Laurie Popp HT (ASCP) Mayo Clinic, Rochester, MN From gu.lang <@t> gmx.at Sun Mar 9 03:22:57 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sun Mar 9 03:23:05 2008 Subject: AW: [Histonet] Washing out formalin fixation (Lengthy) In-Reply-To: <5b6eb13e0803081600q7561e64csb48bc2453d0a6cee@mail.gmail.com> Message-ID: <000001c881be$c84e8ea0$eeeea8c0@dielangs.at> I think one reason for washing the fixed specimen in the "histo-beginning" was to keep the following chemicals clean. Nowadays there's no problem to get new alcohols in good quality for the infiltration-instruments. And I think, that washing wasn't excluded to formalin-fixation. A positive effect of washing should also be the better stainability with acid dyes. The "old" histologists certainly had good reasons (and plenty of time) for this procedure, but today "time is more money". Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Mark Tarango Gesendet: Sonntag, 09. M?rz 2008 01:01 An: histonet@lists.utsouthwestern.edu Betreff: Re: [Histonet] Washing out formalin fixation (Lengthy) I just want to point out again that this works poorly and wastes water... I don't want anyone to get too excited and ditch the HIER buffers. Nobody does this in the real world. It all goes back to what Ren? said, "It (pretty much) doesn't work that way." On Tue, Mar 4, 2008 at 7:56 AM, Bryan Hewlett wrote: > Hi Teri and everyone else on this thread, > > Washing out many of the effects of formaldehyde fixation on tissues with > running water has been known for years (much longer than this old boy has > been around). > In modern terms, it is the essential underlying mechanism for so-called > antigen retrieval (HIER). > > Formaldehyde fixes proteins by addition, with the formation of > hydroxymethyl > adducts on the reactive side chains of proteins. > Once enough of these hydroxymethyl adducts are formed, and IF they are in > close approximation to each other, they may slowly cross-link by the > formation of methylene bridges. > However, these adducts and initial cross-links are unstable and readily > reversed by water and alcohol (see Kiernan (1999). > It takes 24 hours at room temperature for all the hydroxymethyl adducts to > form, i.e. maximal binding threshold (see Fox et al, 1985). > If the tissue is then exposed to running water before all the adducts have > formed (i.e. less than 24 hours), the reversal is very rapid. > The shorter the time in formaldehyde, the more rapid the reversal. > Even after the essential 24 hours fixation and also after a more lengthy 6 > days fixation, running water will still remove the adducts and hydrolyse > the > methylene bridges. > There is at least one publication (Helander, 1994) that provides data > regarding this effect. > After 24 hours fixation, 50% reversal occurred in less than 24 hours, 90% > reversal was obtained after 6 days washing and for 6 days fixation 90% > reversal after 4 weeks washing. > It should be noted that these reversal times were obtained at ambient > temperatures and the times may be considerably reduced by elevated > temperatures. > > This reversal effect is also obtained on tissue sections that have been > processed to wax. > However, because of the additional shrinkage and hydrophobicity of the > processed proteins, the reversal is slowed somewhat until the proteins > re-hydrate. > The reversal effect can also be aided by the presence of other ions in the > water (the purpose of HIER buffers). > Back in the sixties, in order to successfully demonstrate Ig's by IF, we > were reversing the fixation effects on paraffin sections by placing them > in > hypotonic buffers for 2 days at 37C. > Today, since we are all in a great rush for results, we obviously drive > the > reversal at higher temperatures to speed things up!! > > References: > > Baker JR (1958), Principles of Biological Microtechnique, Methuen & Co. > Ltd. > Hopwood D. Fixatives and fixation: A review. Histochemical journal (1969); > 1, p19-55 > Burnett MG. The mechanism of the formaldehyde clock reaction: Methylene > glycol dehydration. J Chem. educ. (1982); 59, 160 > Fox CH et al. Formaldehyde fixation. J Histochem Cytochem (1985); 33, > 845-853 > Helander KG. Kinetic studies of formaldehyde binding in tissue. > Biotechnique > and Histochemistry. (1994); 69, 177-179 > Kiernan J.A., Histological and Histochemical Methods: Theory and Practice, > 3rd Edition, (1999). Oxford: Butterworth-Heinemann. ISBN # 0-7506-3106-6. > Shi SR, Gu J. and Taylor CR. Antigen Retrieval Techniques: > Immunohistochemistry and Molecular Morphology. (2000) Eaton Publishing. > ISBN > 1-881299-43-0. > Sompuram SR, Vani K, Messana E and Bogen SA. A molecular mechanism of > formalin fixation and antigen retrieval. Am J Clin Pathol (2004);121: > 190-199 > > > Best regards, > > Bryan > > ----- Original Message ----- > From: "Johnson, Teri" > To: > Sent: Monday, March 03, 2008 2:33 PM > Subject: [Histonet] Washing out formalin fixation > > > Last week, a researcher here asked me what the chemical mechanism was of > washing out the effects of formalin fixation on the tissues with running > water. In other words, how does it work? Anybody here know? > > Teri Johnson, HT(ASCP)QIHC > Managing Director Histology Facility > Stowers Institute for Medical Research > 1000 E. 50th St. > Kansas City, MO 64110 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jerry <@t> ralambusa.com Sun Mar 9 10:45:57 2008 From: Jerry <@t> ralambusa.com (Jerry Helisek) Date: Sun Mar 9 10:46:01 2008 Subject: [Histonet] RE: Histonet Digest, Vol 52, Issue 12 Message-ID: <3855F92002259948A66A8CA2D16E3A4F05AC60@server.ralambusa.com> sharon, We/RA Lamb have a couple of models of slide dryers depending on your needs and we also have a fantastic tracking system "LambTrack" see the following links and if you want any further information please feel free to contact me outside of Histonet. http://www.ralamb.co.uk/advanced_search_result.php?keywords=e28&x=0&y=0 http://www.ralamb.co.uk/product_info.php?products_id=314 http://www.ralamb.co.uk/product_info.php?products_id=607 Thanks and good luck!! ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ Message: 8 Date: Fri, 7 Mar 2008 14:39:28 -0600 From: "Genest, Sharon SktnHR" Subject: [Histonet] Slide Dryers To: Message-ID: <1C152FCB03A4F84197818DEE847F0D4A03B44E12@stampy.sktnhr.ca> Content-Type: text/plain; charset="us-ascii" Does anyone have a model of slide dryer that works well for them and a supplier name. We a presently using small individual dryers. I can't seem to find any of these and am unsure how people are tracking slides in and out of the larger dryers where multiple users would use the same dryer. Sharon Genest MLT Acting Technologist III Histology Saskatoon Health Region Phone: (306)655-8197 Email: sharon.genest@saskatoonhealthregion.ca From bhewlett <@t> cogeco.ca Sun Mar 9 11:50:11 2008 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Sun Mar 9 11:50:18 2008 Subject: [Histonet] Washing out formalin fixation (Lengthy) References: <000d01c87e10$49506080$6500a8c0@mainbox> <5b6eb13e0803081600q7561e64csb48bc2453d0a6cee@mail.gmail.com> Message-ID: <000301c88205$a461a850$6500a8c0@mainbox> Mark, Nobody is suggesting this as THE way to retrieve tissue antigenicity. Just pointing out the mechanism of the effect in answer to the original question. Actually in the real world, it pretty much DOES work this way! Everybody does it everyday when they short fix tissue in NBF and then process it. The reversal occurs in the processing alcohols and then, for some proteins, something even worse happens, they re-fix in alcohol! The shorter the fixation time in NBF, the more rapidly the reversal occurs and the more like alcohol fixation is the end result. Alcohol fixation can result in the loss of up to 40% of tissue proteins. If your protein of interest is one of them, this can mean that IHC demonstration of the affected protein is compromised and no further amount of HIER is helpful. This combined mixed pattern of formaldehyde and alcohol fixation is responsible for 90% of IHC staining problems. Regards, Bryan ----- Original Message ----- From: "Mark Tarango" To: Sent: Saturday, March 08, 2008 8:00 PM Subject: Re: [Histonet] Washing out formalin fixation (Lengthy) I just want to point out again that this works poorly and wastes water... I don't want anyone to get too excited and ditch the HIER buffers. Nobody does this in the real world. It all goes back to what Ren? said, "It (pretty much) doesn't work that way." On Tue, Mar 4, 2008 at 7:56 AM, Bryan Hewlett wrote: > Hi Teri and everyone else on this thread, > > Washing out many of the effects of formaldehyde fixation on tissues with > running water has been known for years (much longer than this old boy has > been around). > In modern terms, it is the essential underlying mechanism for so-called > antigen retrieval (HIER). > > Formaldehyde fixes proteins by addition, with the formation of > hydroxymethyl > adducts on the reactive side chains of proteins. > Once enough of these hydroxymethyl adducts are formed, and IF they are in > close approximation to each other, they may slowly cross-link by the > formation of methylene bridges. > However, these adducts and initial cross-links are unstable and readily > reversed by water and alcohol (see Kiernan (1999). > It takes 24 hours at room temperature for all the hydroxymethyl adducts to > form, i.e. maximal binding threshold (see Fox et al, 1985). > If the tissue is then exposed to running water before all the adducts have > formed (i.e. less than 24 hours), the reversal is very rapid. > The shorter the time in formaldehyde, the more rapid the reversal. > Even after the essential 24 hours fixation and also after a more lengthy 6 > days fixation, running water will still remove the adducts and hydrolyse > the > methylene bridges. > There is at least one publication (Helander, 1994) that provides data > regarding this effect. > After 24 hours fixation, 50% reversal occurred in less than 24 hours, 90% > reversal was obtained after 6 days washing and for 6 days fixation 90% > reversal after 4 weeks washing. > It should be noted that these reversal times were obtained at ambient > temperatures and the times may be considerably reduced by elevated > temperatures. > > This reversal effect is also obtained on tissue sections that have been > processed to wax. > However, because of the additional shrinkage and hydrophobicity of the > processed proteins, the reversal is slowed somewhat until the proteins > re-hydrate. > The reversal effect can also be aided by the presence of other ions in the > water (the purpose of HIER buffers). > Back in the sixties, in order to successfully demonstrate Ig's by IF, we > were reversing the fixation effects on paraffin sections by placing them > in > hypotonic buffers for 2 days at 37C. > Today, since we are all in a great rush for results, we obviously drive > the > reversal at higher temperatures to speed things up!! > > References: > > Baker JR (1958), Principles of Biological Microtechnique, Methuen & Co. > Ltd. > Hopwood D. Fixatives and fixation: A review. Histochemical journal (1969); > 1, p19-55 > Burnett MG. The mechanism of the formaldehyde clock reaction: Methylene > glycol dehydration. J Chem. educ. (1982); 59, 160 > Fox CH et al. Formaldehyde fixation. J Histochem Cytochem (1985); 33, > 845-853 > Helander KG. Kinetic studies of formaldehyde binding in tissue. > Biotechnique > and Histochemistry. (1994); 69, 177-179 > Kiernan J.A., Histological and Histochemical Methods: Theory and Practice, > 3rd Edition, (1999). Oxford: Butterworth-Heinemann. ISBN # 0-7506-3106-6. > Shi SR, Gu J. and Taylor CR. Antigen Retrieval Techniques: > Immunohistochemistry and Molecular Morphology. (2000) Eaton Publishing. > ISBN > 1-881299-43-0. > Sompuram SR, Vani K, Messana E and Bogen SA. A molecular mechanism of > formalin fixation and antigen retrieval. Am J Clin Pathol (2004);121: > 190-199 > > > Best regards, > > Bryan > > ----- Original Message ----- > From: "Johnson, Teri" > To: > Sent: Monday, March 03, 2008 2:33 PM > Subject: [Histonet] Washing out formalin fixation > > > Last week, a researcher here asked me what the chemical mechanism was of > washing out the effects of formalin fixation on the tissues with running > water. In other words, how does it work? Anybody here know? > > Teri Johnson, HT(ASCP)QIHC > Managing Director Histology Facility > Stowers Institute for Medical Research > 1000 E. 50th St. > Kansas City, MO 64110 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Sun Mar 9 12:57:18 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Sun Mar 9 12:57:24 2008 Subject: [Histonet] Washing out formalin fixation (Lengthy) In-Reply-To: <000301c88205$a461a850$6500a8c0@mainbox> References: <000d01c87e10$49506080$6500a8c0@mainbox> <5b6eb13e0803081600q7561e64csb48bc2453d0a6cee@mail.gmail.com> <000301c88205$a461a850$6500a8c0@mainbox> Message-ID: <5b6eb13e0803091057k6677a7f7w641b5480320ec014@mail.gmail.com> Nobody is suggesting that this is the way to perform antigen retrieval and I wanted to make that clear. What are you talking about anyway? Washing the tissue with water as a method of antigen retrieval or alcohol fixation during processing due to inadequate time in formalin? Find me just a few labs that do antigen retrieval by washing with water for days and then you can say this is how it works in the real world. I gave an answer to the original question too. The question was about reversing the effects of formalin fixation, on tissue, by rinsing with water. You are talking about tissue being fixed by alcohol during processing, due to limited time in formalin (these are different topics). On Sun, Mar 9, 2008 at 9:50 AM, Bryan Hewlett wrote: > Mark, > > Nobody is suggesting this as THE way to retrieve tissue antigenicity. > Just pointing out the mechanism of the effect in answer to the original > question. > > Actually in the real world, it pretty much DOES work this way! > Everybody does it everyday when they short fix tissue in NBF and then > process it. > The reversal occurs in the processing alcohols and then, for some > proteins, > something even worse happens, they re-fix in alcohol! > The shorter the fixation time in NBF, the more rapidly the reversal occurs > and the more like alcohol fixation is the end result. > Alcohol fixation can result in the loss of up to 40% of tissue proteins. > If your protein of interest is one of them, this can mean that IHC > demonstration of the affected protein is compromised and no further amount > of HIER is helpful. > This combined mixed pattern of formaldehyde and alcohol fixation is > responsible for 90% of IHC staining problems. > > > Regards, > > Bryan > > > > > > > > ----- Original Message ----- > From: "Mark Tarango" > To: > Sent: Saturday, March 08, 2008 8:00 PM > Subject: Re: [Histonet] Washing out formalin fixation (Lengthy) > > > I just want to point out again that this works poorly and wastes water... > I > don't want anyone to get too excited and ditch the HIER buffers. Nobody > does this in the real world. It all goes back to what Ren? said, "It > (pretty much) doesn't work that way." > > > On Tue, Mar 4, 2008 at 7:56 AM, Bryan Hewlett wrote: > > > Hi Teri and everyone else on this thread, > > > > Washing out many of the effects of formaldehyde fixation on tissues with > > running water has been known for years (much longer than this old boy > has > > been around). > > In modern terms, it is the essential underlying mechanism for so-called > > antigen retrieval (HIER). > > > > Formaldehyde fixes proteins by addition, with the formation of > > hydroxymethyl > > adducts on the reactive side chains of proteins. > > Once enough of these hydroxymethyl adducts are formed, and IF they are > in > > close approximation to each other, they may slowly cross-link by the > > formation of methylene bridges. > > However, these adducts and initial cross-links are unstable and readily > > reversed by water and alcohol (see Kiernan (1999). > > It takes 24 hours at room temperature for all the hydroxymethyl adducts > to > > form, i.e. maximal binding threshold (see Fox et al, 1985). > > If the tissue is then exposed to running water before all the adducts > have > > formed (i.e. less than 24 hours), the reversal is very rapid. > > The shorter the time in formaldehyde, the more rapid the reversal. > > Even after the essential 24 hours fixation and also after a more lengthy > 6 > > days fixation, running water will still remove the adducts and hydrolyse > > the > > methylene bridges. > > There is at least one publication (Helander, 1994) that provides data > > regarding this effect. > > After 24 hours fixation, 50% reversal occurred in less than 24 hours, > 90% > > reversal was obtained after 6 days washing and for 6 days fixation 90% > > reversal after 4 weeks washing. > > It should be noted that these reversal times were obtained at ambient > > temperatures and the times may be considerably reduced by elevated > > temperatures. > > > > This reversal effect is also obtained on tissue sections that have been > > processed to wax. > > However, because of the additional shrinkage and hydrophobicity of the > > processed proteins, the reversal is slowed somewhat until the proteins > > re-hydrate. > > The reversal effect can also be aided by the presence of other ions in > the > > water (the purpose of HIER buffers). > > Back in the sixties, in order to successfully demonstrate Ig's by IF, we > > were reversing the fixation effects on paraffin sections by placing them > > in > > hypotonic buffers for 2 days at 37C. > > Today, since we are all in a great rush for results, we obviously drive > > the > > reversal at higher temperatures to speed things up!! > > > > References: > > > > Baker JR (1958), Principles of Biological Microtechnique, Methuen & Co. > > Ltd. > > Hopwood D. Fixatives and fixation: A review. Histochemical journal > (1969); > > 1, p19-55 > > Burnett MG. The mechanism of the formaldehyde clock reaction: Methylene > > glycol dehydration. J Chem. educ. (1982); 59, 160 > > Fox CH et al. Formaldehyde fixation. J Histochem Cytochem (1985); 33, > > 845-853 > > Helander KG. Kinetic studies of formaldehyde binding in tissue. > > Biotechnique > > and Histochemistry. (1994); 69, 177-179 > > Kiernan J.A., Histological and Histochemical Methods: Theory and > Practice, > > 3rd Edition, (1999). Oxford: Butterworth-Heinemann. ISBN # > 0-7506-3106-6. > > Shi SR, Gu J. and Taylor CR. Antigen Retrieval Techniques: > > Immunohistochemistry and Molecular Morphology. (2000) Eaton Publishing. > > ISBN > > 1-881299-43-0. > > Sompuram SR, Vani K, Messana E and Bogen SA. A molecular mechanism of > > formalin fixation and antigen retrieval. Am J Clin Pathol (2004);121: > > 190-199 > > > > > > Best regards, > > > > Bryan > > > > ----- Original Message ----- > > From: "Johnson, Teri" > > To: > > Sent: Monday, March 03, 2008 2:33 PM > > Subject: [Histonet] Washing out formalin fixation > > > > > > Last week, a researcher here asked me what the chemical mechanism was of > > washing out the effects of formalin fixation on the tissues with running > > water. In other words, how does it work? Anybody here know? > > > > Teri Johnson, HT(ASCP)QIHC > > Managing Director Histology Facility > > Stowers Institute for Medical Research > > 1000 E. 50th St. > > Kansas City, MO 64110 > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From rydomsal <@t> yahoo.com Sun Mar 9 20:26:26 2008 From: rydomsal <@t> yahoo.com (Ryan Dominique Salazar) Date: Sun Mar 9 20:26:32 2008 Subject: [Histonet] eyeball holder Message-ID: <902718.4835.qm@web52311.mail.re2.yahoo.com> Hi Histopeeps! Can you provide us a protocol for processing eyeball specimen for primates/rodents? Wer'e conducting a study for opthalmology and we're starting to validate some procedures which you might help. We're fixing them in davidson's fluid but we're not sure how many days? Do we need an eyeball holder for embedding them? Thanks a lot guys and have a blessed weekday ahead. Ryan Dominique Salazar Maccine Pte Ltd --------------------------------- Never miss a thing. Make Yahoo your homepage. From JMacDonald <@t> mtsac.edu Sun Mar 9 21:23:15 2008 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Sun Mar 9 21:23:17 2008 Subject: [Histonet] job needed Message-ID: I have a student who has experience in both the clinical lab and the histology lab looking for a job in the LA/Inland Empire area. He has relocated from Northern California. He also has his California phlebotomy licence. If you know of an available lab assistant position please contact me and I will put you in touch with the student. Thank you, Jennifer Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu From derek.papalegis <@t> tufts.edu Mon Mar 10 07:38:56 2008 From: derek.papalegis <@t> tufts.edu (Derek Papalegis) Date: Mon Mar 10 07:39:01 2008 Subject: [Histonet] eyeball holder In-Reply-To: <902718.4835.qm@web52311.mail.re2.yahoo.com> References: <902718.4835.qm@web52311.mail.re2.yahoo.com> Message-ID: <47D52BE0.1020406@tufts.edu> Ryan, You should remove the eyes from Davidson's after 24 hours and place them into 70% alcohol until you are ready to process them. I have found this to be the best way to get the best mouse eye sections. Cutting mouse eyes can be very tedious if you need to see particular areas of the eye that are at different levels. I recently had a study where all my sections needed to show the optic nerve head. I found that there really was no easy way to do this since each eye was different in size and I couldn't find any standardization as to how deep to cut into it to show that particular feature. I would recommend taking some practice eyes and cutting those to ensure that your procedures and techniques are suitable. I have never used an eyeball holder and I don't think that they are necessary as long as you leave the optic nerve attached to the eye. This helps with orientation. I hope this helps. Derek Derek Papalegis HT (ASCP) Histotechnician Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 Ryan Dominique Salazar wrote: > Hi Histopeeps! > > Can you provide us a protocol for processing eyeball specimen for primates/rodents? > Wer'e conducting a study for opthalmology and we're starting to validate some procedures which you might help. > We're fixing them in davidson's fluid but we're not sure how many days? > Do we need an eyeball holder for embedding them? > > Thanks a lot guys and have a blessed weekday ahead. > > Ryan Dominique Salazar > Maccine Pte Ltd > > > --------------------------------- > Never miss a thing. Make Yahoo your homepage. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Mon Mar 10 07:55:02 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 10 07:55:05 2008 Subject: [Histonet] eyeball holder In-Reply-To: <902718.4835.qm@web52311.mail.re2.yahoo.com> Message-ID: <863033.19645.qm@web65708.mail.ac4.yahoo.com> If you go to HistoLogic (a regular publication now auspiced by Sakura) and check their archieves, there are from 4 to 5 large articles describing precisely what you are looking for. Ren? J. Ryan Dominique Salazar wrote: Hi Histopeeps! Can you provide us a protocol for processing eyeball specimen for primates/rodents? Wer'e conducting a study for opthalmology and we're starting to validate some procedures which you might help. We're fixing them in davidson's fluid but we're not sure how many days? Do we need an eyeball holder for embedding them? Thanks a lot guys and have a blessed weekday ahead. Ryan Dominique Salazar Maccine Pte Ltd --------------------------------- Never miss a thing. Make Yahoo your homepage. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From mpence <@t> grhs.net Mon Mar 10 08:14:00 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Mar 10 08:14:06 2008 Subject: [Histonet] The Leica Polaris In-Reply-To: <00fc01c88185$58e18fb0$7b48f9d8@CHURCH> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3760@IS-E2K3.grhs.net> >From my short exposure to the Polaris, you may have some loading/time issues if 1). You load the shorter time batch first or 2). The levels of your solutions are running to their point of dilution. Hope this helps in what little I know about the processor. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rebecca Johnson Sent: Saturday, March 08, 2008 7:32 PM To: histonet Subject: [Histonet] The Leica Polaris Has anyone had problems with loading two runs at one time? Also water contamination problems? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Mon Mar 10 08:44:07 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Mar 10 08:44:15 2008 Subject: [Histonet] Re: H&E Stainers In-Reply-To: <47D35C16.3060003@conxis.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3761@IS-E2K3.grhs.net> With all the "negatives" out there about this stainer, why on earth would you even be giving this one a second look? Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Popp Sent: Saturday, March 08, 2008 9:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: H&E Stainers actually we are "test driving" as you will the new Ventana Symphony stainer and have not had a day where we have not gotten a call from Tuscon stating that there is a problem with the stainer since it passed validation. The nice thing is tech support usually calls us right as the computer is starting to error. The bad thing is you have to go back and start your H&E's over from the beginning... destaining and restaining since there is no resume as of yet. Laurie Popp HT (ASCP) Mayo Clinic, Rochester, MN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Mon Mar 10 09:01:17 2008 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Mon Mar 10 09:01:35 2008 Subject: [Histonet] Slide dryer Message-ID: <0E394B648E5284478A6CCB78E5AFDA2705635508@hpes1.HealthPartners.int> We have the slide dryer from MOPEC and LOVE IT!! It has a turntable and, obviously, uses forced air that circulates in it! We can put multiple racks in the dryer and each tech has a timer for the rack they placed in the oven. The product # is: BK200! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From Valerie.Hannen <@t> parrishmed.com Mon Mar 10 10:49:37 2008 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Mon Mar 10 10:49:53 2008 Subject: [Histonet] FW: The results of your email commands Message-ID: <5680DA93771F0C48954CC8D38425E72401AB34C5@ISMAIL.parrishmed.local> -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Sent: Monday, March 10, 2008 9:13 AM To: Hannen, Valerie Subject: The results of your email commands The results of your email command are provided below. Attached is your original message. - Results: Ignoring non-text/plain MIME parts - Unprocessed: =20 My Section chief has asked me to post this question. Does it hurt the paraffin to go from liquid to solid and back again everyday? Our Pathologist is trying to save the hospital money...he has ask us to put our Embedding station in a "Stand-by Mode".=20 =20 Valerie Hannen, MLT(ASCP),HTL,SU(FL) Parrish Medical Center Titusville, Florida =20=20=20=20 ************************************************************************ ***= *********************** =0D "This email is intended solely for the use of the individual to whom it is = addressed and may contain information that is privileged, confidential or o= therwise exempt from disclosure under applicable law. If the reader of this= email is not the intended recipient or the employee or agent responsible f= or delivering the message to the intended recipient, you are hereby notifie= d that any dissemination, distribution, or copying of this communication is= strictly prohibited. If you have received this communication in error, ple= ase immediately notify us by telephone at 321-268-6167 and return the origi= nal message to us at the listed email address. Thank you - Done. ************************************************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately notify us by telephone at 321-268-6167 and return the original message to us at the listed email address. Thank you From Valerie.Hannen <@t> parrishmed.com Mon Mar 10 10:51:07 2008 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Mon Mar 10 10:51:17 2008 Subject: [Histonet] FW: Pararffin Message-ID: <5680DA93771F0C48954CC8D38425E72401AB34C6@ISMAIL.parrishmed.local> ________________________________ From: Hannen, Valerie Sent: Monday, March 10, 2008 9:12 AM To: 'histonet-request@lists.utsouthwestern.edu' Subject: Pararffin Good Morning fellow histonetters!! My Section chief has asked me to post this question. Does it hurt the paraffin to go from liquid to solid and back again everyday? Our Pathologist is trying to save the hospital money...he has ask us to put our Embedding station in a "Stand-by Mode". Valerie Hannen, MLT(ASCP),HTL,SU(FL) Parrish Medical Center Titusville, Florida ************************************************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately notify us by telephone at 321-268-6167 and return the original message to us at the listed email address. Thank you From doug <@t> ppspath.com Mon Mar 10 12:06:35 2008 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Mon Mar 10 11:08:16 2008 Subject: [Histonet] Re: H&E Stainers In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3761@IS-E2K3.grhs.net> Message-ID: Just remember. There are three sides to every story. There are certain instruments that I would never use that people love. I have an instrument that people say they hate but it works for me. It never hurts to demo a piece of equipment. This should be standard practice before purchasing an instrument. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Monday, March 10, 2008 8:44 AM To: Laurie Popp; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: H&E Stainers With all the "negatives" out there about this stainer, why on earth would you even be giving this one a second look? Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Popp Sent: Saturday, March 08, 2008 9:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: H&E Stainers actually we are "test driving" as you will the new Ventana Symphony stainer and have not had a day where we have not gotten a call from Tuscon stating that there is a problem with the stainer since it passed validation. The nice thing is tech support usually calls us right as the computer is starting to error. The bad thing is you have to go back and start your H&E's over from the beginning... destaining and restaining since there is no resume as of yet. Laurie Popp HT (ASCP) Mayo Clinic, Rochester, MN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SharonC <@t> CarolinasPathology.com Mon Mar 10 11:11:13 2008 From: SharonC <@t> CarolinasPathology.com (Sharon Campbell) Date: Mon Mar 10 11:10:20 2008 Subject: [Histonet] cytology equipment to sell Message-ID: Hello everyone, I have several cytology items for sale. These items are Labconco Biosafety cabinet, cytec thin prep, centrifuge, and vortexor. If interested, I have pictures. Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 (704) 549-8444 x100 sharonc@carolinaspathology.com From beverly <@t> histologytechservices.com Mon Mar 10 11:54:39 2008 From: beverly <@t> histologytechservices.com (Beverly Robinson) Date: Mon Mar 10 11:47:17 2008 Subject: [Histonet] FW: Pararffin In-Reply-To: <5680DA93771F0C48954CC8D38425E72401AB34C6@ISMAIL.parrishmed.local> Message-ID: <0MKpCa-1JYl9V1Vte-0007Dn@mrelay.perfora.net> Cheeeep!! For the very little it costs to keep paraffin melted overnight vs the wear and tear on your embedding unit to have to melt down the paraffin daily and the loss of quality in your paraffin I would think that in the long run you would have saved money by keeping your machine on. We own our own lab and try to save every penney, but have never considered your pathologist's suggestion and at this point never will. Paraffin does break down faster when it is heated and remelted even once. Beverly Robinson Laboratory Manager Histology Tech Services, Inc. Phone: 352-338-0045 FAX: 352-338-0027 This email and its attachments (if any) contain confidential and privileged information. Any dissemination or use of this information by a person other than the intended recipient is unauthorized and may be illegal. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Monday, March 10, 2008 11:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Pararffin ________________________________ From: Hannen, Valerie Sent: Monday, March 10, 2008 9:12 AM To: 'histonet-request@lists.utsouthwestern.edu' Subject: Pararffin Good Morning fellow histonetters!! My Section chief has asked me to post this question. Does it hurt the paraffin to go from liquid to solid and back again everyday? Our Pathologist is trying to save the hospital money...he has ask us to put our Embedding station in a "Stand-by Mode". Valerie Hannen, MLT(ASCP),HTL,SU(FL) Parrish Medical Center Titusville, Florida **************************************************************************** ********************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately notify us by telephone at 321-268-6167 and return the original message to us at the listed email address. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From beverly <@t> histologytechservices.com Mon Mar 10 11:57:36 2008 From: beverly <@t> histologytechservices.com (Beverly Robinson) Date: Mon Mar 10 11:50:19 2008 Subject: [Histonet] Slide dryer In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA2705635508@hpes1.HealthPartners.int> Message-ID: <0MKpCa-1JYlCM3U6x-0007Iy@mrelay.perfora.net> Ditto to the Mopec slides dryer! The best I've ever used! Works great are considering buying another one in the near future. Beverly Robinson HT(ASCP) Laboratory Manager Histology Tech Services, Inc. Phone: 352-338-0045 FAX: 352-338-0027 This email and its attachments (if any) contain confidential and privileged information. Any dissemination or use of this information by a person other than the intended recipient is unauthorized and may be illegal. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Monday, March 10, 2008 10:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide dryer We have the slide dryer from MOPEC and LOVE IT!! It has a turntable and, obviously, uses forced air that circulates in it! We can put multiple racks in the dryer and each tech has a timer for the rack they placed in the oven. The product # is: BK200! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From beverly <@t> histologytechservices.com Mon Mar 10 13:01:57 2008 From: beverly <@t> histologytechservices.com (Beverly Robinson) Date: Mon Mar 10 12:54:39 2008 Subject: [Histonet] FW: Pararffin In-Reply-To: <0MKpCa-1JYl9V1Vte-0007Dn@mrelay.perfora.net> Message-ID: <0MKpCa-1JYmCh0MDG-0007Mg@mrelay.perfora.net> Hey Guys! Other than the fact that I can't spell penny correctly sometimes, I'd like to apologize for my strong reaction to the email about putting the embedding center on "stand by". I had a pathologist once who occasionally walked thru the lab after we had gone home for the day and turn off all the equipment, especially the embedding center. Sorry! For the Cheeeep! Comment also. Beverly Robinson HT(ASCP) Laboratory Manager Histology Tech Services, Inc. Phone: 352-338-0045 FAX: 352-338-0027 This email and its attachments (if any) contain confidential and privileged information. Any dissemination or use of this information by a person other than the intended recipient is unauthorized and may be illegal. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beverly Robinson Sent: Monday, March 10, 2008 12:55 PM To: 'Hannen, Valerie'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] FW: Pararffin Cheeeep!! For the very little it costs to keep paraffin melted overnight vs the wear and tear on your embedding unit to have to melt down the paraffin daily and the loss of quality in your paraffin I would think that in the long run you would have saved money by keeping your machine on. We own our own lab and try to save every penney, but have never considered your pathologist's suggestion and at this point never will. Paraffin does break down faster when it is heated and remelted even once. Beverly Robinson Laboratory Manager Histology Tech Services, Inc. Phone: 352-338-0045 FAX: 352-338-0027 This email and its attachments (if any) contain confidential and privileged information. Any dissemination or use of this information by a person other than the intended recipient is unauthorized and may be illegal. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Monday, March 10, 2008 11:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Pararffin ________________________________ From: Hannen, Valerie Sent: Monday, March 10, 2008 9:12 AM To: 'histonet-request@lists.utsouthwestern.edu' Subject: Pararffin Good Morning fellow histonetters!! My Section chief has asked me to post this question. Does it hurt the paraffin to go from liquid to solid and back again everyday? Our Pathologist is trying to save the hospital money...he has ask us to put our Embedding station in a "Stand-by Mode". Valerie Hannen, MLT(ASCP),HTL,SU(FL) Parrish Medical Center Titusville, Florida **************************************************************************** ********************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately notify us by telephone at 321-268-6167 and return the original message to us at the listed email address. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tim.morken <@t> thermofisher.com Mon Mar 10 12:35:01 2008 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Mon Mar 10 13:06:39 2008 Subject: [Histonet] Two positions open at ThermoFisher Scientific, Immunohistochemistry, Fremont CA Message-ID: <6BFF6D137DF6BC43B33891BA96E83B19013E9630@PGHCR-EXMB-VS-1.na.fshrnet.com> Two positions are currently open at ThermoFisher Scientific Immunohistochemistry facility in Fremont, CA (San Franscico Bay Area). 1) Technical Support Representative, Immunohistochemistry 2) Quality Control Scientist, Immunohistochemistry Details of each below. Contact: Britt Tasker 269-544-5735 Britt.tasker@thermofisher.com ************************************************************************ ********************** Technical Support Representative, Immunohistochemistry Requirements: Expertise Scientific Research & Development Education Bachelors Job Type Full-time Location United States - California - Fremont Job Level Experienced Description: This position provides technical service and support, customer training and Sales support for the APFremont immunohistochemistry reagent and instrument product line from the home office and at customer sites to customers, Distributors and Field Sales and Service. Responds to and resolves customer and Field Sales/Service inquiries on the telephone, through on-line networks, or by mail or fax. Provides troubleshooting and resolution of customers technical issues, and provides help in understanding of the appropriate use of Lab Vision reagent and instrument products. Handles requests for the replacement of apparently defective product. Tests defective product to determine root cause of failure. Completes, maintains, and processes relevant paperwork and documentation, including records of customer complaints. Provides periodic reports to management including compilations of customer complaints, trend analyses, and suggestions for improvement. Maintains up-to-date sources of reference materials, articles and technical manuals as required to provide current information. Reviews available technical literature to maintain an awareness of current laboratory practices and procedures. Provides on-site product installation and customer training, on an as-needed basis. Participates in validating new products, customer evaluations of existing products and application support. BA/BS in biochemistry, medical technology or biology with 2-4 years relevant experience using clinical diagnostic products in the laboratory. Experience in immunohistochemistry, histology and the microscopic identification of tissues preferred. Excellent communication and customer skills, as well as strong planning, organization, analytical and time management skills. Must be computer proficient. Willingness to travel up to 15% of the time. ************************************************************************ ******************** Quality Control Scientist, Immunohistochemistry Requirements: Expertise Quality Assurance Education Bachelors Job Type Full-time Location United States - California - Fremont Job Level Experienced Description: This position reviews and approves IHC slides for product release and lot verification. Provides guidance on troubleshooting of routine QC tests. Assists the Product Surveillance (stability testing) program. Assists in developing, designing, and performing experimental strategies and procedures for projects. Evaluates ideas that can lead to new or improved products or processes. Provides for the development and training of QC-Research Associates and part-time histotechnician. Assists compliance with GMP, QSRs, and ISO regulations in the performance of department responsibilities. Supports the transfer and release of new products. Provides feedback on regular QC progress to upper management. Assists in developing various assays and transfers the technology to Manufacturing. Qualified candidates will have a BA/BS or above degree in biological sciences or related discipline plus a minimum of five (5) to six (6) years of pathology, histology, and/or surgical pathology experience in a diagnostic, pharmaceutical, medical device or biotechnology environment. Requires extensive knowledge and skill in cell biology processes, histology, protein localization in tissue, pathology, and IHC reagent technology. Must be able to effectively communicate and interact with others. Demonstrated success in technical proficiency, scientific creativity, and independent thought. Proven skills in interpretation of scientific literature and interaction with co-workers and other departments. Strong organizational skills to be able to multi-task. Skilled at analyzing data and summarizing results. Strong troubleshooting, verbal, and communication skills, as well as documentation of results and procedures. Strong word processing, spreadsheet, and imaging skills. Ability to use statistical techniques and experimental design. In-depth knowledge of IHC reagent technology and tissue staining techniques. Understanding of protein, enzyme, and antibody functions and structures. Contact: Britt Tasker 269-544-5735 Britt.tasker@thermofisher.com ************************************************************************ **************************************** Tim Morken Technical Support Manager Lab Vision Products Anatomical Pathology ThermoFisher Scientific 47777 Warm Springs Blvd. Fremont, CA 94539 USA tel: 01-510-991-2840 fax: 01 510-991-2826 Tim.Morken@thermofisher.com web: www.labvision.com www.thermofisher.com The World Leader in Serving Science From rjbuesa <@t> yahoo.com Mon Mar 10 14:59:42 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 10 14:59:47 2008 Subject: [Histonet] FW: Pararffin In-Reply-To: <5680DA93771F0C48954CC8D38425E72401AB34C6@ISMAIL.parrishmed.local> Message-ID: <231348.27741.qm@web65705.mail.ac4.yahoo.com> Absolutely not! In the same way that may melt lead time and again. Ren? J. "Hannen, Valerie" wrote: ________________________________ From: Hannen, Valerie Sent: Monday, March 10, 2008 9:12 AM To: 'histonet-request@lists.utsouthwestern.edu' Subject: Pararffin Good Morning fellow histonetters!! My Section chief has asked me to post this question. Does it hurt the paraffin to go from liquid to solid and back again everyday? Our Pathologist is trying to save the hospital money...he has ask us to put our Embedding station in a "Stand-by Mode". Valerie Hannen, MLT(ASCP),HTL,SU(FL) Parrish Medical Center Titusville, Florida ************************************************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately notify us by telephone at 321-268-6167 and return the original message to us at the listed email address. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From Barry.R.Rittman <@t> uth.tmc.edu Mon Mar 10 15:07:57 2008 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Mon Mar 10 15:08:04 2008 Subject: [Histonet] FW: Pararffin In-Reply-To: <231348.27741.qm@web65705.mail.ac4.yahoo.com> References: <5680DA93771F0C48954CC8D38425E72401AB34C6@ISMAIL.parrishmed.local> <231348.27741.qm@web65705.mail.ac4.yahoo.com> Message-ID: A minor point. If paraffin is heated for extended periods of time or at too high a temperature then the lower melting point components may be lost. This is probably not significant in the normal path lab but may be of importance if large volumes of paraffin are kept heated as a reserve. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, March 10, 2008 3:00 PM To: Hannen, Valerie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] FW: Pararffin Absolutely not! In the same way that may melt lead time and again. Ren? J. "Hannen, Valerie" wrote: ________________________________ From: Hannen, Valerie Sent: Monday, March 10, 2008 9:12 AM To: 'histonet-request@lists.utsouthwestern.edu' Subject: Pararffin Good Morning fellow histonetters!! My Section chief has asked me to post this question. Does it hurt the paraffin to go from liquid to solid and back again everyday? Our Pathologist is trying to save the hospital money...he has ask us to put our Embedding station in a "Stand-by Mode". Valerie Hannen, MLT(ASCP),HTL,SU(FL) Parrish Medical Center Titusville, Florida ************************************************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately notify us by telephone at 321-268-6167 and return the original message to us at the listed email address. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Mar 10 16:17:24 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 10 16:17:31 2008 Subject: [Histonet] FW: Pararffin In-Reply-To: Message-ID: <498135.62988.qm@web65713.mail.ac4.yahoo.com> We used a paraffin bath with a capacity of 15 lbs, that had to be refilled (with about 7-9 lbs daily). To that usage level is to the one I am referring to, and that cannot affect the paraffin characteristics. Ren? J. "Rittman, Barry R" wrote: A minor point. If paraffin is heated for extended periods of time or at too high a temperature then the lower melting point components may be lost. This is probably not significant in the normal path lab but may be of importance if large volumes of paraffin are kept heated as a reserve. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, March 10, 2008 3:00 PM To: Hannen, Valerie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] FW: Pararffin Absolutely not! In the same way that may melt lead time and again. Ren? J. "Hannen, Valerie" wrote: ________________________________ From: Hannen, Valerie Sent: Monday, March 10, 2008 9:12 AM To: 'histonet-request@lists.utsouthwestern.edu' Subject: Pararffin Good Morning fellow histonetters!! My Section chief has asked me to post this question. Does it hurt the paraffin to go from liquid to solid and back again everyday? Our Pathologist is trying to save the hospital money...he has ask us to put our Embedding station in a "Stand-by Mode". Valerie Hannen, MLT(ASCP),HTL,SU(FL) Parrish Medical Center Titusville, Florida ************************************************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately notify us by telephone at 321-268-6167 and return the original message to us at the listed email address. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From akemiat3377 <@t> yahoo.com Mon Mar 10 17:42:44 2008 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Mon Mar 10 17:42:49 2008 Subject: [Histonet] Re: H&E Stainers In-Reply-To: <47D35C16.3060003@conxis.com> Message-ID: <611831.11613.qm@web31313.mail.mud.yahoo.com> For a test drive it sounds like a lot of additional work. I hope your using tests cases instead of actual patient cases. This sounds like a huge delay for H&E's to be read out. Haven't your pathologists complained. Akemi --- Laurie Popp wrote: > actually we are "test driving" as you will the new > Ventana Symphony > stainer and have not had a day where we have not > gotten a call from > Tuscon stating that there is a problem with the > stainer since it passed > validation. The nice thing is tech support usually > calls us right as > the computer is starting to error. The bad thing is > you have to go back > and start your H&E's over from the beginning... > destaining and > restaining since there is no resume as of yet. > > Laurie Popp HT (ASCP) > Mayo Clinic, Rochester, MN > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Akemi Allison-Tacha, BS, HT(ASCP)HTL Client Services Manager PhenoPath laboratories 551 North 34th Street, Suite 100 Seattle, WA 98103-8675 Work: (206) 374-9000 Cell: (425) 941-4287 E-Mail: akemiat3377@yahoo.com From pereirafamily <@t> cox.net Mon Mar 10 20:51:16 2008 From: pereirafamily <@t> cox.net (pereirafamily) Date: Mon Mar 10 20:51:19 2008 Subject: [Histonet] automated coverslipper Message-ID: <005101c8831a$64f7f820$316a0744@pereirafarm> Hi all, Our facility needs to get a new automated coverslipper and were wondering if anyone could recommend or not recommend one. thanks. From Tony_Reilly <@t> health.qld.gov.au Tue Mar 11 01:06:17 2008 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Tue Mar 11 01:06:47 2008 Subject: [Histonet] H & E stainers In-Reply-To: References: Message-ID: <47D6ADF7.471C.0039.0@health.qld.gov.au> As Douglas states every body will have their own preferences and needs and your selection should be based on your own needs. Ignoring the brand of the instrument if you want speed a linear batch stainer will always be faster than an XY stainer which gets slower with each basket added. An XY stainer offers more versatility as it can be used for multiple programs and staining protocols. If you want a compromise of both there are some stainers like the Leica ST40 which are a linear stainer with 2 tracks and variable programming. Determine your needs then trial the models that will best suit your personal needs to find the model of your preference. regards Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_reilly@health.qld.gov.au >>> "Hineline, Paula" 13/02/2008 6:02 am >>> Hello, We are researching histology H&E stainers for our lab. We have gotten quotes on the Leica ST 5020 multistainer and the Leica ST 4040 linear stainer. Recommendations would be appreciated. Thanks Paula Hineline, HT ASCP Pathology Laboratory phineline@grandpath.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/ received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. **************************************************************** From ivananikic <@t> yahoo.com Tue Mar 11 04:43:05 2008 From: ivananikic <@t> yahoo.com (Ivana Nikic) Date: Tue Mar 11 04:43:09 2008 Subject: [Histonet] Antibody penetration issues Message-ID: <939836.18650.qm@web50401.mail.re2.yahoo.com> Hello everybody, I am having some difficulties with my stainings in the mouse spinal cord. I am using longitudinal, both vibratome and cryo-, 4%PFA-fixed sections. The problem is that, with protocols I have tried so far, using longitudinal sections results in a non-uniform staining pattern. It works pretty fine in the grey matter, but the penetration in the white matter axons is pretty low. I am trying to work this out by using tissue from transgenic mouse lines with fluorescently labelled neurons and neuronal mitochondria and anti-GFP antibody. I have tried X-100 Triton (0.1-1%) permeabilization and several fixatives - methanol, methanol+acetone, ethanol-acetic acid. It helps with the axons, but it is still hard to get the antibody into mitochondria. Any feedback would be greatly appreciated ! Thanks a lot in advance, Ivana --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From cmiller <@t> physlab.com Tue Mar 11 07:54:43 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Tue Mar 11 07:54:03 2008 Subject: [Histonet] H&E control slide In-Reply-To: <476270.64906.qm@web32606.mail.mud.yahoo.com> References: <476270.64906.qm@web32606.mail.mud.yahoo.com> Message-ID: <001101c88377$139a3800$3d02a8c0@plab.local> We use gallbladder or appendix Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicholas Rosenbaum Sent: Thursday, March 06, 2008 5:33 PM To: Histonet Subject: Re: [Histonet] H&E control slide We use uterus. Nicholas Rosenbaum, HT ASCP University of Virginia Medical Center ----- Original Message ---- From: Angela Bitting To: histonet@lists.utsouthwestern.edu Sent: Wednesday, March 5, 2008 11:48:37 AM Subject: [Histonet] H&E control slide What is the consensus on what tissue to use for an H&E control. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -----Inline Attachment Follows----- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD -----Inline Attachment Follows----- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________ ________ Looking for last minute shopping deals? Find them fast with Yahoo! Search. http://tools.search.yahoo.com/newsearch/category.php?category=shopping _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From rjbuesa <@t> yahoo.com Tue Mar 11 08:01:31 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 11 08:01:39 2008 Subject: [Histonet] automated coverslipper In-Reply-To: <005101c8831a$64f7f820$316a0744@pereirafarm> Message-ID: <673925.62449.qm@web65709.mail.ac4.yahoo.com> If you want a FILM coverslipper, Sakura is the one to buy. If you want a GLASS coverslipper Leica OR Sakura would be 2 options. Ren? J. pereirafamily wrote: Hi all, Our facility needs to get a new automated coverslipper and were wondering if anyone could recommend or not recommend one. thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From TMcNemar <@t> lmhealth.org Tue Mar 11 08:16:12 2008 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Tue Mar 11 08:15:23 2008 Subject: [Histonet] Grossing Tech (sorry) Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F542@lmhsmail.lmhealth.org> I know this has been discussed many times but I am unable to find anything in the archives that seems to fit my situation so please forgive me for bringing it up again.... I am negotiating a deal with our 3 pathologists to take over the grossing of small biopsies (skins, GIs, etc.) so I have to come up with a price. I am also a full-time lab employee. I really cannot see the lab bumping up my hourly pay to cover work that is done for the pathologists (they are hospital employees but separate from the lab. On the other hand, the time that I spend grossing will take away from my lab duties. I don't know how it's all going to work out yet.... At any rate, I have to come up with a price. It seems reasonable that the best scenario for me is to be paid per specimen grossed based on CPT code. The vast majority of our specimen are 88305s. If I can go this route, I am thinking somewhere in the neighborhood of $2 for an 88304 and $3 for an 88305. Perhaps this is way off-base and totally unrealistic. I'm hoping that someone out there has worked out something similar...... Tom. From JCollins <@t> palmbeachpath.com Tue Mar 11 08:45:48 2008 From: JCollins <@t> palmbeachpath.com (Judy Collins) Date: Tue Mar 11 08:45:55 2008 Subject: [Histonet] Grossing Tech Message-ID: <05CAE76AB5D5ED409864C6DD86F13349238CEC6C@pbpsflexch02.pbp.local> Our grossing techs usually gross up to 50 specimens per hour (most are 88305) so at your suggested rate, they would be paid $400.00 per day. That would be $50.00 per hour. That seems unreasonable. Judy Collins Palm Beach Pathology From Rcartun <@t> harthosp.org Tue Mar 11 08:57:54 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Mar 11 08:58:18 2008 Subject: [Histonet] Biospecimen banking coordinator Message-ID: <47D657A2020000770000B412@gwmail4.harthosp.org> I am currently investigating the creation and implementation of a "biospecimen bank" here at Hartford Hospital. I would appreciate hearing from anyone already doing this regarding salary structure for a coordinator and a tissue collection specialist. Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From trathborne <@t> somerset-healthcare.com Tue Mar 11 09:12:26 2008 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Mar 11 09:12:36 2008 Subject: [Histonet] automated coverslipper In-Reply-To: <005101c8831a$64f7f820$316a0744@pereirafarm> Message-ID: We are very pleased with our CV5030 from Leica. We used this by itself for over a year before we were able to purchase the ST5020. I highly recommend both units for anyone looking. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of pereirafamily Sent: Monday, March 10, 2008 9:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] automated coverslipper Hi all, Our facility needs to get a new automated coverslipper and were wondering if anyone could recommend or not recommend one. thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. From victor <@t> pathology.washington.edu Tue Mar 11 09:29:08 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Tue Mar 11 09:29:17 2008 Subject: [Histonet] automated coverslipper In-Reply-To: References: Message-ID: <47D69734.3090206@pathology.washington.edu> One of our facilities is evaluating the CV5030 from Leica and so far they are very impressed. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Rathborne, Toni wrote: > We are very pleased with our CV5030 from Leica. We used this by itself for over a year before we were able to purchase the ST5020. I highly recommend both units for anyone looking. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of > pereirafamily > Sent: Monday, March 10, 2008 9:51 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] automated coverslipper > > > Hi all, > > Our facility needs to get a new automated coverslipper and were wondering if anyone could recommend or not recommend one. thanks. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Somerset Medical Center is proud to receive the Somerset County > Business Partnership's 2006 Quality of Life Award. > > ------------------------------------------------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From froyer <@t> bitstream.net Tue Mar 11 09:46:22 2008 From: froyer <@t> bitstream.net (Ford Royer) Date: Tue Mar 11 09:47:04 2008 Subject: [Histonet] FW: Pararffin In-Reply-To: <0MKpCa-1JYl9V1Vte-0007Dn@mrelay.perfora.net> References: <5680DA93771F0C48954CC8D38425E72401AB34C6@ISMAIL.parrishmed.local> <0MKpCa-1JYl9V1Vte-0007Dn@mrelay.perfora.net> Message-ID: <008601c88386$adb5ac80$7701a80a@Ford> Beverly makes a definitive point here. Turning off an embedding center every night, allowing the paraffin to solidify; then turning it back on in the morning and forcing it melt the solidified paraffin will do more stress to the embedding center than to the paraffin involved (assuming that you keep your paraffin temps. within reason). When the reservoir is up to temp, it only takes a few intermittent electrical pulses to the heating elements to keep it "at temp". Making the embedding center heat and melt solid paraffin every single morning will take years off the life of the instrument's heating elements. Keeping it on all the time (and "At Temperature") uses about the same amount of energy as a 40 watt light bulb in a 24 hour period. The only time I could see that it would make sense to turn the unit off is if you do not work weekends or holidays ("3-day weekends") Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL:? 612-839-1046 Phone:? 763-542-8725 Fax:? 763-546-4830 eMail:? clinicallab@minnesotamedical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beverly Robinson Sent: Monday, March 10, 2008 11:55 AM To: 'Hannen, Valerie'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] FW: Pararffin Cheeeep!! For the very little it costs to keep paraffin melted overnight vs the wear and tear on your embedding unit to have to melt down the paraffin daily and the loss of quality in your paraffin I would think that in the long run you would have saved money by keeping your machine on. We own our own lab and try to save every penney, but have never considered your pathologist's suggestion and at this point never will. Paraffin does break down faster when it is heated and remelted even once. Beverly Robinson Laboratory Manager Histology Tech Services, Inc. Phone: 352-338-0045 FAX: 352-338-0027 This email and its attachments (if any) contain confidential and privileged information. Any dissemination or use of this information by a person other than the intended recipient is unauthorized and may be illegal. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Monday, March 10, 2008 11:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Pararffin ________________________________ From: Hannen, Valerie Sent: Monday, March 10, 2008 9:12 AM To: 'histonet-request@lists.utsouthwestern.edu' Subject: Pararffin Good Morning fellow histonetters!! My Section chief has asked me to post this question. Does it hurt the paraffin to go from liquid to solid and back again everyday? Our Pathologist is trying to save the hospital money...he has ask us to put our Embedding station in a "Stand-by Mode". Valerie Hannen, MLT(ASCP),HTL,SU(FL) Parrish Medical Center Titusville, Florida **************************************************************************** ********************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately notify us by telephone at 321-268-6167 and return the original message to us at the listed email address. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Tue Mar 11 11:04:06 2008 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Mar 11 10:05:29 2008 Subject: SPAM-LOW: [Histonet] Grossing Tech In-Reply-To: <05CAE76AB5D5ED409864C6DD86F13349238CEC6C@pbpsflexch02.pbp.local> Message-ID: If it were you grossing the specimens for that price would it seem unreasonable? :) Take What You Can, Give Nothing Back! Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Judy Collins Sent: Tuesday, March 11, 2008 8:46 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Grossing Tech Our grossing techs usually gross up to 50 specimens per hour (most are 88305) so at your suggested rate, they would be paid $400.00 per day. That would be $50.00 per hour. That seems unreasonable. Judy Collins Palm Beach Pathology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Tue Mar 11 10:05:58 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Mar 11 10:06:08 2008 Subject: AW: [Histonet] Biospecimen banking coordinator In-Reply-To: <47D657A2020000770000B412@gwmail4.harthosp.org> Message-ID: <000901c88389$6a38ba80$eeeea8c0@dielangs.at> Richard, would you be so kind and explain to me, what a biospecimen bank is? Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Richard Cartun Gesendet: Dienstag, 11. M?rz 2008 14:58 An: Histonet Betreff: [Histonet] Biospecimen banking coordinator I am currently investigating the creation and implementation of a "biospecimen bank" here at Hartford Hospital. I would appreciate hearing from anyone already doing this regarding salary structure for a coordinator and a tissue collection specialist. Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmjohnson34 <@t> hotmail.com Tue Mar 11 10:15:55 2008 From: jmjohnson34 <@t> hotmail.com (Jennifer Johnson) Date: Tue Mar 11 10:16:04 2008 Subject: [Histonet] CAP question Message-ID: I noticed on this years CAP Checklist that Quality Management had replaced Quality Assurance and Quality Control. Is it necessary to change these terms on all of the procedures year after year? Thanks, Jennifer _________________________________________________________________ Shed those extra pounds with MSN and The Biggest Loser! http://biggestloser.msn.com/ From esther.peters <@t> verizon.net Tue Mar 11 10:31:42 2008 From: esther.peters <@t> verizon.net (Esther Peters) Date: Tue Mar 11 10:35:08 2008 Subject: [Histonet] [Fwd: urgent H&E issues] Message-ID: <47D6A5DE.1080101@verizon.net> I'm passing this question along from a research colleague, because I have never worked with plastic (I'm not sure whether she is using MMA or GMA, either). I tried a Google search but did not see a direct answer. We look forward to your assistance! We have been cutting some plastic here over the last few days (with a borrowed microtome), and are having some difficulties with the H&E staining. It seems that the section gets tons of little bubbles under the plastic (or so it seems). We do not see such bubbles when we stain with basic fuchsin and think maybe its due to the alcohol or xylenes used at the end of the H&E. We are not removing the plastic (methacrylate). Do you have any ideas or can track down an H&E staining procedure especially for plastic? Esther Peters, Ph.D. George Mason University epeters2@gmu.edu From JCollins <@t> palmbeachpath.com Tue Mar 11 10:50:15 2008 From: JCollins <@t> palmbeachpath.com (Judy Collins) Date: Tue Mar 11 10:50:25 2008 Subject: [Histonet] Grossing Techs Message-ID: <05CAE76AB5D5ED409864C6DD86F13349238CEC76@pbpsflexch02.pbp.local> Yes, it would still seem unreasonable. I don't agree with your "take what you can, give nothing back" statement. Judy Collins If it were you grossing the specimens for that price would it seem unreasonable? :) Take What You Can, Give Nothing Back! Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 From llewllew <@t> shaw.ca Tue Mar 11 10:57:05 2008 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Tue Mar 11 10:57:22 2008 Subject: [Histonet] [Fwd: urgent H&E issues] References: <47D6A5DE.1080101@verizon.net> Message-ID: <000d01c88390$8e0487d0$bd144246@yourlk4rlmsu> When I used to do this with 1 micron sections of kidney in methyl/butyl methacrylate I would cut the section with a 20% acetone filled trough attached. I picked up and floated onto a water bath kept at 65 C, lined several section up and mounted on the slide. I baked on for at least a couple of hours. To stain, I removed the plastic with an hour in acetone, xylene or, preferably, chloroform at room temperature. I used toluidine blue for the stain. H&E came out very pale, even if I used Cole's hemalum for an hour at 60 C with no differentiation. I found that sections thicker than 1 micron were increasingly more difficult to deal with as the section thickness increased. 3 microns was the practical limit unless I was willing to spend a lot of time fiddling around. However, the whole point of such sections was to cut them thinner than I could from a paraffin block, which I also had from a second biopsy (and a third for EM).. Bryan Llewellyn ----- Original Message ----- From: "Esther Peters" To: Sent: Tuesday, March 11, 2008 8:31 AM Subject: [Histonet] [Fwd: urgent H&E issues] > I'm passing this question along from a research colleague, because I have > never worked with plastic (I'm not sure whether she is using MMA or GMA, > either). I tried a Google search but did not see a direct answer. We look > forward to your assistance! > > We have been cutting some plastic here over the last few days (with a > borrowed microtome), and are having some difficulties with the H&E > staining. It seems that the section gets tons of little bubbles under > the plastic (or so it seems). We do not see such bubbles when we stain > with basic fuchsin and think maybe its due to the alcohol or xylenes used > at the end of the H&E. We are not removing the plastic (methacrylate). > Do you have any ideas or can track down an H&E staining procedure > especially for plastic? > Esther Peters, Ph.D. > George Mason University > epeters2@gmu.edu > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMyers1 <@t> aol.com Tue Mar 11 11:05:38 2008 From: JMyers1 <@t> aol.com (JMyers1@aol.com) Date: Tue Mar 11 11:05:49 2008 Subject: [Histonet] Antibody penetration issues Message-ID: Ivana: Have you considered performing any pretreatment procedures, like enzymatic digestion or heat-retrieval? Joe ------------------------------ Message: 9 Date: Tue, 11 Mar 2008 02:43:05 -0700 (PDT) From: Ivana Nikic Subject: [Histonet] Antibody penetration issues To: histonet@lists.utsouthwestern.edu Hello everybody, I am having some difficulties with my stainings in the mouse spinal cord. I am using longitudinal, both vibratome and cryo-, 4%PFA-fixed sections. The problem is that, with protocols I have tried so far, using longitudinal sections results in a non-uniform staining pattern. It works pretty fine in the grey matter, but the penetration in the white matter axons is pretty low. I am trying to work this out by using tissue from transgenic mouse lines with fluorescently labelled neurons and neuronal mitochondria and anti-GFP antibody. I have tried X-100 Triton (0.1-1%) permeabilization and several fixatives - methanol, methanol+acetone, ethanol-acetic acid. It helps with the axons, but it is still hard to get the antibody into mitochondria. Any feedback would be greatly appreciated ! Thanks a lot in advance, Ivana **************It's Tax Time! Get tips, forms, and advice on AOL Money & Finance. (http://money.aol.com/tax?NCID=aolprf00030000000001) From doug <@t> ppspath.com Tue Mar 11 12:07:38 2008 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Mar 11 11:08:54 2008 Subject: SPAM-LOW: [Histonet] Grossing Techs In-Reply-To: <05CAE76AB5D5ED409864C6DD86F13349238CEC76@pbpsflexch02.pbp.local> Message-ID: Where has the humor gone? Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Judy Collins Sent: Tuesday, March 11, 2008 10:50 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Grossing Techs Yes, it would still seem unreasonable. I don't agree with your "take what you can, give nothing back" statement. Judy Collins If it were you grossing the specimens for that price would it seem unreasonable? :) Take What You Can, Give Nothing Back! Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From John.Auld <@t> whnt.nhs.uk Tue Mar 11 11:13:46 2008 From: John.Auld <@t> whnt.nhs.uk (John Auld) Date: Tue Mar 11 11:13:54 2008 Subject: [Histonet] Re: Automated coverslipper Message-ID: Hi ThermoFisher have a newish model out, ClearVue. We beta tested one for a while and bought a production model as son as we could. Whatever you do try as many as poss before you buy. John Message: 7 Date: Mon, 10 Mar 2008 18:51:16 -0700 From: "pereirafamily" Subject: [Histonet] automated coverslipper To: Message-ID: <005101c8831a$64f7f820$316a0744@pereirafarm> Content-Type: text/plain; charset="iso-8859-1" Hi all, Our facility needs to get a new automated coverslipper and were wondering if anyone could recommend or not recommend one. thanks. From MadaryJ <@t> MedImmune.com Tue Mar 11 11:29:26 2008 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Tue Mar 11 11:29:56 2008 Subject: [Histonet] Paraffin on off a good thing and rotation of paraffins Message-ID: Personally the fact that the timers are even on the embedders points to the notion of embedders on a timer being a good thing. We melt down blocks and re-embed them with no damage. (Well maybe Nocito doesn't have to.) I do drive domestive cars but have to give it to the Japanese and Germans when it comes to our histo equipment. They put those timers on there to save electricity and overheating of paraffin. I think at least putting the embedder on a weekend timer is a start. I have been doing that for years and I never have paraffin issues. We should have a healthy respect for paraffin overheating and mixing of old and new. I think there is plenty of evidence that if you placed paraffin in a dispenser on heat indefinitely that eventually it would lose some of the properties we want in a paraffin. So I think the green aspect is great but I have also been a firm believer in not overheating paraffin. That segways into adding new paraffin to old paraffin. Folks who keep adding paraffin to paraffin in the embedder not allowing it to truly get empty conceivably are mxing paraffins months or even years old because it never gets truly empty. Paraffins in the embedder at least the part that makes the actual blocks really should be one lot heated and cooled at the same time. Once it is empty then add new paraffin. Actually I am so weird that when I rotate the processor paraffins I drain everything from the embedder and use the embedder paraffin in the rotation process. This allows for the freshest paraffin in the embedder. Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Laboratory One Medimmune Way Gaithersburg, MD 20878 ph 301.398.4745/6360 fx 301.398.9745 To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From marktarango <@t> gmail.com Tue Mar 11 11:44:38 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Tue Mar 11 11:44:48 2008 Subject: [Histonet] Grossing Tech In-Reply-To: <05CAE76AB5D5ED409864C6DD86F13349238CEC6C@pbpsflexch02.pbp.local> References: <05CAE76AB5D5ED409864C6DD86F13349238CEC6C@pbpsflexch02.pbp.local> Message-ID: <5b6eb13e0803110944u42314590k432553935920ac07@mail.gmail.com> What he is thinking sounds perfectly resonable to me. Imagine if the pathologists had to hire a company to come in and gross for them. We are living in a time of obscene corporate greed. Can you imagine how much that company would charge? These pathologists are getting the grossing done by someone they trust and are familar with. $50.00/hr doesn't seem like that much money to me. Why not pay them what they're worth? The pathologists are still getting quite a deal if you ask me. On Tue, Mar 11, 2008 at 6:45 AM, Judy Collins wrote: > Our grossing techs usually gross up to 50 specimens per hour (most are > 88305) so at your suggested rate, they would be paid $400.00 per day. That > would be $50.00 per hour. That seems unreasonable. > > Judy Collinsy > Palm Beach Pathology > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From POWELL_SA <@t> Mercer.edu Tue Mar 11 11:41:08 2008 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Tue Mar 11 11:45:36 2008 Subject: [Histonet] Deadline correction for Region III hotel registration Message-ID: <01MSANIOVSEI000CQ6@Macon2.Mercer.edu> Subject: Reminder for Region III Hi Guys, March 13th, Thursday, is the latest deadline for making your reservations for Region III meeting hosted by GSH. The Westin Peachtree Plaza has extended the deadline for registration for the Region III meeting in Atlanta Georgia April 3-5, 2008. Take advantage of the discounted rate of $129 single, double, triple or quad. The phone number to the Westin is 1-404-659-1400 and please state that you are attending the GSH/Region III meeting. Program Registration Deadline to avoid a late fee is March 21st. The program can be downloaded from our website at www.histosearch.com/gsh. Click on the symposium link to get a PDF file of the program. Vendors have a link to their registration form to exhibit at the meeting on the same page. If you have questions Chris Coley, GSH Exhibit Liaison, has contact information is on that form. There has been some confusion about the workshop fees. The $35 registration fee is nonrefundable and payable by everyone. This covers the seminars and Friday lunch, but the workshops, #1 through #5 are $40 each for members, $55 each for Nonmembers (either NSH or GSH) and $25 each for students (requires signature of program director). Workshops 1 and 2 will run concurrently and only one of them can be taken. Number 3 is Saturday morning, no conflicting workshop, only seminars. Workshops 4 and 5 are in the afternoon running concurrently so only one of them can be taken. I hope this clears up any questions you may have. If anyone has further questions please feel to contact me at this email address. Come to Atlanta and join us for a great meeting. Shirley Powell GSH Secretary/Registrar From Annette.Fletcher <@t> providence.org Tue Mar 11 11:47:09 2008 From: Annette.Fletcher <@t> providence.org (Fletcher, Annette M) Date: Tue Mar 11 11:47:19 2008 Subject: [Histonet] Leadership opportunities in Oregon Message-ID: <284596E350CB0743BB500B18475E7A0B720C8A@wn1223.or.providence.org> Dear Histonetters, Providence Health and Services is a well-respected, not-for-profit Organization that rewards it's employees with highly competitive compensation and excellent benefits. Our state of the art lab has recently acquired an Express Microwave Processor for Histotechnology and 3 new Ventana Immunohistostainers. We service 6 Hospitals and generate 55,000 surgicals per year (averaging 750 blocks per day, high volume IHC's (40,000 per year). We have three key Leadership opportunities available: * Assistant Technical Supervisor/Histology * Supervisorr/Regional Histology * Pathology Manager If you or any of your Colleagues would enjoy learning more about these opportunities in the Pacific NW, please call me at 503-215-5840 or email me at annette.fletcher@providence.org. Our established presence throughout the Pacific NW offers the quality of life you deserve. Amid lush greenery, a gentle sea breeze or a backdrop of a majestic mountain range, you'll find the best of city or rural living with great neighborhoods, outdoor adventures and cultural activities. Have a great week, Annette Fletcher Senior Recruiter Providence Regional Employment 1235 NE 47th Avenue, Ste 200 Portland, OR 97214 (503) 215-5840 annette.fletcher@providence.org DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From JCollins <@t> palmbeachpath.com Tue Mar 11 11:50:56 2008 From: JCollins <@t> palmbeachpath.com (Judy Collins) Date: Tue Mar 11 11:51:11 2008 Subject: [Histonet] Grossing Tech In-Reply-To: <5b6eb13e0803110944u42314590k432553935920ac07@mail.gmail.com> References: <05CAE76AB5D5ED409864C6DD86F13349238CEC6C@pbpsflexch02.pbp.local> <5b6eb13e0803110944u42314590k432553935920ac07@mail.gmail.com> Message-ID: <05CAE76AB5D5ED409864C6DD86F13349238CEC7A@pbpsflexch02.pbp.local> You missed my second post. $50.00 per hour was incorrect. The figure is actually $150.00. Judy Collins ________________________________ From: Mark Tarango [mailto:marktarango@gmail.com] Sent: Tuesday, March 11, 2008 12:45 PM To: Judy Collins Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Grossing Tech What he is thinking sounds perfectly resonable to me. Imagine if the pathologists had to hire a company to come in and gross for them. We are living in a time of obscene corporate greed. Can you imagine how much that company would charge? These pathologists are getting the grossing done by someone they trust and are familar with. $50.00/hr doesn't seem like that much money to me. Why not pay them what they're worth? The pathologists are still getting quite a deal if you ask me. On Tue, Mar 11, 2008 at 6:45 AM, Judy Collins > wrote: Our grossing techs usually gross up to 50 specimens per hour (most are 88305) so at your suggested rate, they would be paid $400.00 per day. That would be $50.00 per hour. That seems unreasonable. Judy Collinsy Palm Beach Pathology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charles.Embrey <@t> carle.com Tue Mar 11 11:57:42 2008 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Tue Mar 11 11:58:14 2008 Subject: [Histonet] Grossing Tech (sorry) In-Reply-To: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F542@lmhsmail.lmhealth.org> Message-ID: <44780C571F28624DBB446DE55C4D733A1FE596@EXCHANGEBE1.carle.com> Tom, You have to remember that you are not only taking on increased duties but increased responsibilities and liabilities. What you are suggesting is not unreasonable or outrageous. If you didn't do it they would have to do it themselves or hire a PA. If you are doing PA type work then you should demand a PA like salary and what you are asking would be in that ballpark. Of course a PA would gross more than just the 304 and 305 specimens but your availability should count for something. It never hurts to ask for what you want. Good Luck Charles Embrey, PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Tuesday, March 11, 2008 8:16 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Grossing Tech (sorry) I know this has been discussed many times but I am unable to find anything in the archives that seems to fit my situation so please forgive me for bringing it up again.... I am negotiating a deal with our 3 pathologists to take over the grossing of small biopsies (skins, GIs, etc.) so I have to come up with a price. I am also a full-time lab employee. I really cannot see the lab bumping up my hourly pay to cover work that is done for the pathologists (they are hospital employees but separate from the lab. On the other hand, the time that I spend grossing will take away from my lab duties. I don't know how it's all going to work out yet.... At any rate, I have to come up with a price. It seems reasonable that the best scenario for me is to be paid per specimen grossed based on CPT code. The vast majority of our specimen are 88305s. If I can go this route, I am thinking somewhere in the neighborhood of $2 for an 88304 and $3 for an 88305. Perhaps this is way off-base and totally unrealistic. I'm hoping that someone out there has worked out something similar...... Tom. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Tue Mar 11 12:12:19 2008 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Tue Mar 11 12:12:28 2008 Subject: [Histonet] H&E control tissue Message-ID: <0E394B648E5284478A6CCB78E5AFDA2705635520@hpes1.HealthPartners.int> "The best control is healthy skin with the connective tissue in place but a tonsil is also great for checking how well your lymph tissue is staining. Tonsil should be cut thinner just like the lymph nodes so you should use the tonsil when staining lymph nodes. Make sure the tonsil has connective tissue in it so you can see the eosin staining in the collagen, blood vessels, cytoplasm, and RBC's. Another good tissue is cervix with the glands. The glandular area gives you the nice hematoxylin staining and the muscle and collagen fibers give you a nice contrast on the eosin staining plus you can always get cervix. I would stay away from the appendix since there is usually a lot of necrosis and the placenta is a little to bloody so the eosin is always some what overwhelming for a good evaluation " This was given to me by Diane Sterchi who has many years of experience in various histology settings!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From SBarnes <@t> elch.org Tue Mar 11 13:38:46 2008 From: SBarnes <@t> elch.org (Sue Barnes) Date: Tue Mar 11 13:41:29 2008 Subject: [Histonet] TBS/OLYMPUS MICROTOME USERS Message-ID: <807C106E9A171C46B0CF253C2507971E15AC41@elchex01.elch.net> I would like to hear from anyone using the TBS/OLYMPUS Cut 4060 microtome with the Cut 2020B knife plate. It is for high profile blades. We have been having problems and can't seem to get it right. Thanks Sue Barnes East Liverpool City Hospital East Liverpool, Ohio From Jenee.S.Odani <@t> hawaii.gov Tue Mar 11 14:50:14 2008 From: Jenee.S.Odani <@t> hawaii.gov (Jenee.S.Odani@hawaii.gov) Date: Tue Mar 11 14:50:35 2008 Subject: [Histonet] Good Lab Practices ? Message-ID: Hi all, Is anyone willing to share your manual/protocols regarding histo processing and especially disposal of fixed tissues and blocks? Thank you! Jenee S. Odani, D.V.M., Dipl. ACVP Veterinary Medical Officer Hawaii State Veterinary Laboratory/DAI 99-941 Halawa Valley Street, Aiea, HI, 96701 Phone: (808) 483-7131/Fax: (808) 483-7110 From Margaret.Perry <@t> sdstate.edu Tue Mar 11 14:54:24 2008 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Tue Mar 11 14:54:34 2008 Subject: [Histonet] CAP and timers Message-ID: We are a vet diagnostic lab and are moving to a system of accreditation similar to CAP. We are working to comply with OIE, NCCLS standards. I looked in the archives but found very little on how other labs pass the CAP or JACHO requirements for timers? Do most labs throw timers away and get new or do you use some way to calibrate the timers? Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 From Janet.Bonner <@t> FLHOSP.ORG Tue Mar 11 15:31:21 2008 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Tue Mar 11 15:32:06 2008 Subject: [Histonet] CAP question References: Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F257C@fhosxchmb006.ADVENTISTCORP.NET> No, just every ten years. @:) ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jennifer Johnson Sent: Tue 3/11/2008 11:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP question I noticed on this years CAP Checklist that Quality Management had replaced Quality Assurance and Quality Control. Is it necessary to change these terms on all of the procedures year after year? Thanks, Jennifer _________________________________________________________________ Shed those extra pounds with MSN and The Biggest Loser! http://biggestloser.msn.com/_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From tkngflght <@t> yahoo.com Tue Mar 11 16:10:37 2008 From: tkngflght <@t> yahoo.com (Cheryl) Date: Tue Mar 11 16:10:48 2008 Subject: [Histonet] Looking for Christie Gowan-- In-Reply-To: <5F31F38C96781A4FBE3196EBC22D47807F257C@fhosxchmb006.ADVENTISTCORP.NET> References: <5F31F38C96781A4FBE3196EBC22D47807F257C@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: <00f401c883bc$5b8f7e00$6901a8c0@FSROGER> Hi everyone! I'm trying to touch base with a tech by the name of Christie Gowan--she used to work at a lab in the Northeast and I've seen her post recently--any help is appreciated. Thanks! Cheryl Cheryl R. Kerry, HT(ASCP), BA Full Staff Inc. Staffing the lab - One GREAT tech at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone admin@fullstaff.org www.fullstaff.org From mpence <@t> grhs.net Tue Mar 11 16:41:48 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Mar 11 16:41:57 2008 Subject: [Histonet] Used Tissue Processor for sale Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A376A@IS-E2K3.grhs.net> I have a used Sakura Tissue-Tek VIP E300 processor for sale that was purchased in Nov. 1996. It has had very little work done to it. This is a real "work horse" of a processor and would be great for a small lab or as a reliable back-up. You will buy this processor "As Is" and there are no promises, but I can tell you it was working fine the day we stopped using it. The buyer is responsible for the crating and arrangement of shipping. Picture for serious inquires available on request. I am starting to sound like a salesman. Contact off-line would be best. Mike Pence AP Supervisor Great River Medical Center West Burlington,Ia (319) 768-4546 mpence@grhs.net From jnocito <@t> satx.rr.com Tue Mar 11 17:27:42 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Mar 11 17:27:50 2008 Subject: [Histonet] Grossing Tech References: <05CAE76AB5D5ED409864C6DD86F13349238CEC6C@pbpsflexch02.pbp.local><5b6eb13e0803110944u42314590k432553935920ac07@mail.gmail.com> <05CAE76AB5D5ED409864C6DD86F13349238CEC7A@pbpsflexch02.pbp.local> Message-ID: <009601c883c7$1f913640$0302a8c0@yourxhtr8hvc4p> yeah, but with the price of gas going the it is, he might be on to something JTT ----- Original Message ----- From: "Judy Collins" To: "Mark Tarango" Cc: Sent: Tuesday, March 11, 2008 11:50 AM Subject: RE: [Histonet] Grossing Tech You missed my second post. $50.00 per hour was incorrect. The figure is actually $150.00. Judy Collins ________________________________ From: Mark Tarango [mailto:marktarango@gmail.com] Sent: Tuesday, March 11, 2008 12:45 PM To: Judy Collins Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Grossing Tech What he is thinking sounds perfectly resonable to me. Imagine if the pathologists had to hire a company to come in and gross for them. We are living in a time of obscene corporate greed. Can you imagine how much that company would charge? These pathologists are getting the grossing done by someone they trust and are familar with. $50.00/hr doesn't seem like that much money to me. Why not pay them what they're worth? The pathologists are still getting quite a deal if you ask me. On Tue, Mar 11, 2008 at 6:45 AM, Judy Collins > wrote: Our grossing techs usually gross up to 50 specimens per hour (most are 88305) so at your suggested rate, they would be paid $400.00 per day. That would be $50.00 per hour. That seems unreasonable. Judy Collinsy Palm Beach Pathology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.reilly <@t> jcu.edu.au Tue Mar 11 19:22:35 2008 From: laurie.reilly <@t> jcu.edu.au (Laurie Reilly) Date: Tue Mar 11 19:24:17 2008 Subject: [Histonet] TBS/OLYMPUS MICROTOME USERS In-Reply-To: <807C106E9A171C46B0CF253C2507971E15AC41@elchex01.elch.net> References: <807C106E9A171C46B0CF253C2507971E15AC41@elchex01.elch.net> Message-ID: <000b01c883d7$2caef550$5255db89@health.ad.jcu.edu.au> We have one of these microtomes, although it is branded "microtec" It has a 2020A1 low profile blade holder and we have it set at 15 on the scale to get satisfactory sectioning. Maybe this will help. Regards, Laurie. Mr. Laurie REILLY Histopathology School of Veterinary and Biomedical Sciences James Cook University Townsville Qld. 4811 Australia. Phone 07 4781 4468 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Barnes Sent: Wednesday, 12 March 2008 4:39 AM To: Histonet (E-mail) Subject: [Histonet] TBS/OLYMPUS MICROTOME USERS I would like to hear from anyone using the TBS/OLYMPUS Cut 4060 microtome with the Cut 2020B knife plate. It is for high profile blades. We have been having problems and can't seem to get it right. Thanks Sue Barnes East Liverpool City Hospital East Liverpool, Ohio _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Tue Mar 11 17:40:41 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Mar 11 20:09:02 2008 Subject: [Histonet] Quality of equipment Message-ID: <00d201c883c8$efb84d30$0302a8c0@yourxhtr8hvc4p> I must air a pet peeve that has come up recently. Have you noticed since all the buy-outs, hostile takeovers and mergers that the quality of the equipment has deteriorated? We purchased two new stainers and received them in December 2007. Since we received them, either one or both have been broken for one reason or another. I'm talking major problems like the machine getting hung up, the arm loosing its position and coming down on top of a reagent container, parts falling off, etc. To the company's credit, they did replace one machine this week, but, did this need to happen at all? We have had a demo coverslipper for 5 weeks that we can't use because it is broken and the company hasn't sent out anyone to fix it. Hmmmmmmm, makes me want to go out and purchase two of these models. At my last place of employment, two of the same machines kept breaking down. I had (no exaggeration) 1/2 inch of repair receipts with in one year and as far as I know, that machine is still there. These are different machines from different companies. It seems that the quality is just not there any more. Is anyone else experiencing this or is it just a Nocito thing? I know, I left myself open on that one. Joe "the Toe" please don't flame me Nocito From anthony <@t> histotechexchange.com Tue Mar 11 20:19:01 2008 From: anthony <@t> histotechexchange.com (anthony@histotechexchange.com) Date: Tue Mar 11 20:36:42 2008 Subject: [Histonet] Re: Processing fatty breast tissue and fatty derm tissue In-Reply-To: References: Message-ID: <1472.65.40.219.161.1205284741.squirrel@host7.wfdns.com> Dear Ramona: I am late to the game so I am sorry if you have already had this one. I have never done this, so please try it on some fatty tissue that you are going to throw away and secondly I have no idea how it will affect antigen retrieval or anything involving antibodies. With that out of the way you may want to try taking the specimen through alcohols then onto the first clearing agent, then back to alcohol and then back into the clearing agents and on to wax. Good luck on finding the times etc. Can anyone else out there give your opinion on this practice. Yours truly, Anthony Williams BSc. HT Histotech Exchange LLC 19 Whitmore St. Lexington, VA 24450 T 1 (302) 383 9780 F 1 (540) 463 3583 anthony@histotechexchange.com Dear Ramona: I am late to the game so I am sorry if you have already had this one. I have neve done this, so please try it on some lipoma that you are going to throw away and secondly I have no idea how it will affect antigen retreval or anthing involving antibodies. > I also would like some advice on processing fatty tissue. I have tried > using Pen-Fix prior to processing, but my pathologist complain about an > artifact and refuse to use it anymore. Can't explain that one. I am > currently processing with formalin x2, 70% x2, 95% x2, 100% x2, clearing > x2. I thought the dehydration step should be increased on the fatty > tissue, but you are saying that is not the problem? Can someone else > please offer some experience on this issue. > > Ramona Turner, HT (ASCP) > Potomac Hospital > Woodbridge, VA > _________________________________________________________________ > Climb to the top of the charts!?Play the word scramble challenge with star > power. > http://club.live.com/star_shuffle.aspx?icid=starshuffle_wlmailtextlink_jan_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mickie25 <@t> netzero.net Tue Mar 11 21:14:38 2008 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Tue Mar 11 21:15:22 2008 Subject: [Histonet] Used Tissue Processor for sale In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A376A@IS-E2K3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838C017A376A@IS-E2K3.grhs.net> Message-ID: HI Histonetters, The Advanced Mohs Training Seminar slated for April 10th & 11th at Belair Instrument Company in Springfield, NJ still has a couple of spaces available. The deadline for registration is March 21st. The block of hotel rooms will be given away on March 15th (possibly extended to March 21). If you have any interest in honing your Mohs histology skills and/or certifying your Mohs expertise, give me a call. Thank you. Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net From Tony_Reilly <@t> health.qld.gov.au Tue Mar 11 21:43:15 2008 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Tue Mar 11 21:43:45 2008 Subject: [Histonet] Re: Processing fatty breast tissue and fatty derm tissue In-Reply-To: <1472.65.40.219.161.1205284741.squirrel@host7.wfdns.com> References: <1472.65.40.219.161.1205284741.squirrel@host7.wfdns.com> Message-ID: <47D7CFE1.471C.0039.0@health.qld.gov.au> Hi Anthony This is a common practice in many laboratories. The problem that can be encountered is that some modern processors will not allow it as they are not configured for an alcohol station after a xylene. The times should be no different to any other fatty run which usually have longer times than your routine processing program. regards Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_reilly@health.qld.gov.au >>> 12/03/2008 11:19 am >>> Dear Ramona: I am late to the game so I am sorry if you have already had this one. I have never done this, so please try it on some fatty tissue that you are going to throw away and secondly I have no idea how it will affect antigen retrieval or anything involving antibodies. With that out of the way you may want to try taking the specimen through alcohols then onto the first clearing agent, then back to alcohol and then back into the clearing agents and on to wax. Good luck on finding the times etc. Can anyone else out there give your opinion on this practice. Yours truly, Anthony Williams BSc. HT Histotech Exchange LLC 19 Whitmore St. Lexington, VA 24450 T 1 (302) 383 9780 F 1 (540) 463 3583 anthony@histotechexchange.com Dear Ramona: I am late to the game so I am sorry if you have already had this one. I have neve done this, so please try it on some lipoma that you are going to throw away and secondly I have no idea how it will affect antigen retreval or anthing involving antibodies. > I also would like some advice on processing fatty tissue. I have tried > using Pen-Fix prior to processing, but my pathologist complain about an > artifact and refuse to use it anymore. Can't explain that one. I am > currently processing with formalin x2, 70% x2, 95% x2, 100% x2, clearing > x2. I thought the dehydration step should be increased on the fatty > tissue, but you are saying that is not the problem? Can someone else > please offer some experience on this issue. > > Ramona Turner, HT (ASCP) > Potomac Hospital > Woodbridge, VA > _________________________________________________________________ > Climb to the top of the charts! Play the word scramble challenge with star > power. > http://club.live.com/star_shuffle.aspx?icid=starshuffle_wlmailtextlink_jan_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/ received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. **************************************************************** From raj <@t> bluemarble.net Tue Mar 11 21:56:19 2008 From: raj <@t> bluemarble.net (Rebecca Johnson) Date: Tue Mar 11 21:56:24 2008 Subject: Fw: Fw: [Histonet] The Leica Polaris Message-ID: <011f01c883ec$a5cbda60$7b48f9d8@CHURCH> ----- Original Message ----- From: "rocky&venita" To: "Rebecca Johnson" Sent: Tuesday, March 11, 2008 2:59 PM Subject: Re: Fw: [Histonet] The Leica Polaris > ask if they how tell me how to change time on machine and when our 2nd 85% > has 83% pure on it that truly isn't 85%...... sometimes the paraffin has > the marks on it like it needs to be changed when run is done the marks are > gone. done it do that to let you know it's getting low or close to > changing. the girls friday pushed finger on 2 paraffins didn't need > chagning they held finger on it messed it up now we have no paraffin. i > think they talked to william 1 girl said he was in a different mood monday > real smart acting big time. he has the manual in his office locked > up....... > > -----Original Message----- >>From: Rebecca Johnson >>Sent: Mar 10, 2008 8:15 PM >>To: Venita Sheets >>Subject: Fw: [Histonet] The Leica Polaris >> >> >>----- Original Message ----- >>From: "Mike Pence" >>To: "Rebecca Johnson" ; "histonet" >> >>Sent: Monday, March 10, 2008 8:14 AM >>Subject: RE: [Histonet] The Leica Polaris >> >> >>>From my short exposure to the Polaris, you may have some loading/time >>issues if 1). You load the shorter time batch first or 2). The levels >>of your solutions are running to their point of dilution. Hope this >>helps in what little I know about the processor. >> >>Mike >> >>-----Original Message----- >>From: histonet-bounces@lists.utsouthwestern.edu >>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rebecca >>Johnson >>Sent: Saturday, March 08, 2008 7:32 PM >>To: histonet >>Subject: [Histonet] The Leica Polaris >> >> >> >>Has anyone had problems with loading two runs at one time? >>Also water contamination problems? >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> > > > ________________________________________ > PeoplePC Online > A better way to Internet > http://www.peoplepc.com > From raj <@t> bluemarble.net Tue Mar 11 21:57:26 2008 From: raj <@t> bluemarble.net (Rebecca Johnson) Date: Tue Mar 11 21:57:31 2008 Subject: Fw: Fw: [Histonet] The Leica Polaris Message-ID: <012401c883ec$cd8ba260$7b48f9d8@CHURCH> This is from a fellow histo tech having problems with her Polaris Thanks for all of your advice ----- Original Message ----- From: "rocky&venita" To: "Rebecca Johnson" Sent: Tuesday, March 11, 2008 3:01 PM Subject: Re: Fw: [Histonet] The Leica Polaris > rebecca ask if you load alot of autopsy blocks if that dilutes the > dilutions......----Original Message----- >>From: Rebecca Johnson >>Sent: Mar 10, 2008 8:18 PM >>To: Venita Sheets >>Subject: Fw: [Histonet] The Leica Polaris >> >> >>----- Original Message ----- >>From: "Beth Delescavage" >>To: "Rebecca Johnson" >>Sent: Monday, March 10, 2008 10:50 AM >>Subject: RE: [Histonet] The Leica Polaris >> >> >>There seems to be a little bit of a delay when you load two runs if you >>want them off at the same time. If you load them at slightly different >>times you should be ok. We did not have water contamination but we were >>using the wrong wax and had infiltration issues. Hope this helps. >> >>Thanks~ >>Beth >>CellNetix Labs, Seattle WA >>-----Original Message----- >>From: histonet-bounces@lists.utsouthwestern.edu >>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rebecca >>Johnson >>Sent: Saturday, March 08, 2008 5:32 PM >>To: histonet >>Subject: [Histonet] The Leica Polaris >> >> >>Has anyone had problems with loading two runs at one time? >>Also water contamination problems? >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >>DISCLAIMER: >>This message is intended for the sole use of the addressee, and may >>contain >>information that is privileged, confidential and exempt from disclosure >>under applicable law. If you are not the addressee you are hereby notified >>that you may not use, copy, disclose, or distribute to anyone the message >>or >>any information contained in the message. If you have received this >>message >>in error, please immediately advise the sender by reply email and delete >>this message. >> >> >> >> > > > ________________________________________ > PeoplePC Online > A better way to Internet > http://www.peoplepc.com > From Tony_Reilly <@t> health.qld.gov.au Tue Mar 11 22:27:27 2008 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Tue Mar 11 22:27:49 2008 Subject: Fw: Fw: [Histonet] The Leica Polaris In-Reply-To: <012401c883ec$cd8ba260$7b48f9d8@CHURCH> References: <012401c883ec$cd8ba260$7b48f9d8@CHURCH> Message-ID: <47D7DA3D.471C.0039.0@health.qld.gov.au> Hi I have been using a Peloris for about 4 years now with excellent results. The point to understand is that the instrument will always use the cleanest/purist of each reagent type last so when 2 runs are going at the same time they will be competing for the same last alcohol, xylene etc. You will run into problems if for example you try to start a 1 hour run two hours after a 4 hour run as it may be delayed due to a clash in choice of reagent. If possible always start a shorter run before a longer run. I have not had a problem with water carryover but I have noticed that when exclusively using the reagent pump in/pump out facility that there is a small amount of reagent which can not be removed by the pump which will over time reduce the purity of reagents, so it is worthwhile to occasionally remove the containers and tip out any residue before refilling. regards Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_reilly@health.qld.gov.au >>> "Rebecca Johnson" 12/03/2008 12:57 pm >>> This is from a fellow histo tech having problems with her Polaris Thanks for all of your advice ----- Original Message ----- From: "rocky&venita" To: "Rebecca Johnson" Sent: Tuesday, March 11, 2008 3:01 PM Subject: Re: Fw: [Histonet] The Leica Polaris > rebecca ask if you load alot of autopsy blocks if that dilutes the > dilutions......----Original Message----- >>From: Rebecca Johnson >>Sent: Mar 10, 2008 8:18 PM >>To: Venita Sheets >>Subject: Fw: [Histonet] The Leica Polaris >> >> >>----- Original Message ----- >>From: "Beth Delescavage" >>To: "Rebecca Johnson" >>Sent: Monday, March 10, 2008 10:50 AM >>Subject: RE: [Histonet] The Leica Polaris >> >> >>There seems to be a little bit of a delay when you load two runs if you >>want them off at the same time. If you load them at slightly different >>times you should be ok. We did not have water contamination but we were >>using the wrong wax and had infiltration issues. Hope this helps. >> >>Thanks~ >>Beth >>CellNetix Labs, Seattle WA >>-----Original Message----- >>From: histonet-bounces@lists.utsouthwestern.edu >>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rebecca >>Johnson >>Sent: Saturday, March 08, 2008 5:32 PM >>To: histonet >>Subject: [Histonet] The Leica Polaris >> >> >>Has anyone had problems with loading two runs at one time? >>Also water contamination problems? >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >>DISCLAIMER: >>This message is intended for the sole use of the addressee, and may >>contain >>information that is privileged, confidential and exempt from disclosure >>under applicable law. If you are not the addressee you are hereby notified >>that you may not use, copy, disclose, or distribute to anyone the message >>or >>any information contained in the message. If you have received this >>message >>in error, please immediately advise the sender by reply email and delete >>this message. >> >> >> >> > > > ________________________________________ > PeoplePC Online > A better way to Internet > http://www.peoplepc.com ( http://www.peoplepc.com/ ) > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/ received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. **************************************************************** From talulahgosh <@t> gmail.com Wed Mar 12 06:51:03 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Mar 12 06:51:16 2008 Subject: Fwd: [Histonet] Antibody penetration issues In-Reply-To: References: <939836.18650.qm@web50401.mail.re2.yahoo.com> Message-ID: Have you tried fixing for less time? Some of our antibodies need 2 hour fixed embryos, as opposed to overnight in paraformaldehyde. Emily -- People aren't like chocolates. People are bastards. Bastards with bastard coating and bastard filling. From JWEEMS <@t> sjha.org Wed Mar 12 07:13:32 2008 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Mar 12 07:16:56 2008 Subject: [Histonet] Quality of equipment References: <00d201c883c8$efb84d30$0302a8c0@yourxhtr8hvc4p> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3204726CAB@sjhaexc02.sjha.org> With the "do more with less" mentality there just aren't enough of us to make sure the quality remains the same. At least that's my take on it. We have the same problems in our lab. The harder I work the behinder I get and the light at the end of the tunnel is probably a train... :>) Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Joe Nocito Sent: Tue 3/11/2008 6:40 PM To: histonet Subject: [Histonet] Quality of equipment I must air a pet peeve that has come up recently. Have you noticed since all the buy-outs, hostile takeovers and mergers that the quality of the equipment has deteriorated? We purchased two new stainers and received them in December 2007. Since we received them, either one or both have been broken for one reason or another. I'm talking major problems like the machine getting hung up, the arm loosing its position and coming down on top of a reagent container, parts falling off, etc. To the company's credit, they did replace one machine this week, but, did this need to happen at all? We have had a demo coverslipper for 5 weeks that we can't use because it is broken and the company hasn't sent out anyone to fix it. Hmmmmmmm, makes me want to go out and purchase two of these models. At my last place of employment, two of the same machines kept breaking down. I had (no exaggeration) 1/2 inch of repair receipts with in one year and as far as I know, that machine is still there. These are different machines from different companies. It seems that the quality is just not there any more. Is anyone else experiencing this or is it just a Nocito thing? I know, I left myself open on that one. Joe "the Toe" please don't flame me Nocito _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From rshooki_99 <@t> yahoo.com Wed Mar 12 07:42:25 2008 From: rshooki_99 <@t> yahoo.com (richard shook) Date: Wed Mar 12 07:42:35 2008 Subject: [Histonet] salery survey Message-ID: <955992.48865.qm@web36103.mail.mud.yahoo.com> A quick question for all is there, or has there been a recent histology salery survey and if so where could i find it. i have seen one before but cant remember where it was thank you rich rabkin derm path ____________________________________________________________________________________ Looking for last minute shopping deals? Find them fast with Yahoo! Search. http://tools.search.yahoo.com/newsearch/category.php?category=shopping From rjbuesa <@t> yahoo.com Wed Mar 12 07:49:13 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 12 07:49:24 2008 Subject: [Histonet] Grossing Tech In-Reply-To: <05CAE76AB5D5ED409864C6DD86F13349238CEC6C@pbpsflexch02.pbp.local> Message-ID: <618962.21391.qm@web65714.mail.ac4.yahoo.com> Judy: Why do you see that "unreasonable"? "Pay by piece" or "by task" is one of the best ways of being paid and assures the highest productivity level, the only draw back is tha it needs a tight QA. Did you know that we in the US are paid less by hour that our Canadian, Australian, British and South African counterparts? That the salary for out histotechs is amongst the worst in the field? That they are linked to the inflation rate and cannot go agove it? Why a work like ours with all the responsibilities it entails has to be lower than that of a good manufacturing machinist? Salaries should be negotiated on the basis of what one thinks is worth. When negotiating a salary you should not consider what others earn, it is YOUR salary and you negotiate it as an individual not as part of a group. If somebody end making $50/hour , DURING SOME HOURS, I don't mind. What one has to do is to come with a similar soluitin to earn more, that is all. Ti me it is perfectly logical to ask for $2 or $3 per specimen. What fraction of the return money the lab gets is that amount? Did you know that for a $850/test FISH the materials costa is less that $25/ test? Why can you not be a participant of that total revenue? Go for it, and ask $2 or $3 per specimen. If you are good at what you do, and your lab administrator is not stingy you will get it! Ren? J. Judy Collins wrote: Our grossing techs usually gross up to 50 specimens per hour (most are 88305) so at your suggested rate, they would be paid $400.00 per day. That would be $50.00 per hour. That seems unreasonable. Judy Collins Palm Beach Pathology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From rjbuesa <@t> yahoo.com Wed Mar 12 07:51:57 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 12 07:52:04 2008 Subject: [Histonet] CAP question In-Reply-To: Message-ID: <584348.47558.qm@web65703.mail.ac4.yahoo.com> The change is motivated by the slowly introduction in our field of the ISO9001:2000 international management standards, where QM = QA + QC You will have to start getting used to those changes because they have been developed as a result of the demands of the World market demands. Ren? J. Jennifer Johnson wrote: I noticed on this years CAP Checklist that Quality Management had replaced Quality Assurance and Quality Control. Is it necessary to change these terms on all of the procedures year after year? Thanks, Jennifer _________________________________________________________________ Shed those extra pounds with MSN and The Biggest Loser! http://biggestloser.msn.com/_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From inno291janet <@t> yahoo.co.uk Wed Mar 12 07:59:55 2008 From: inno291janet <@t> yahoo.co.uk (Innocent Mosha) Date: Wed Mar 12 07:59:59 2008 Subject: [Histonet] I need to purchase used Microtome and used Tissue processing machine. Message-ID: <324816.28641.qm@web25003.mail.ukl.yahoo.com> Hi Histonetors! It is long time I was`nt in touch with you. Am Tanzanian working in National Hospital in Dar Es salaam, Tanzania. Am now thinking to establish a small histology laboratory. Due to economic strains I would like to be assisted to have the Microtome and Tissue processing machine from anyone. I have saved little money to assist the cost. So I bag anyone who had those items fuctioning well regardless of brand name, year of manufacture to help me. Thanking you in advance. Innocnt --------------------------------- Rise to the challenge for Sport Relief with Yahoo! for Good From rjbuesa <@t> yahoo.com Wed Mar 12 08:30:39 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 12 08:30:49 2008 Subject: [Histonet] Re: Processing fatty breast tissue and fatty derm tissue In-Reply-To: <1472.65.40.219.161.1205284741.squirrel@host7.wfdns.com> Message-ID: <28037.70857.qm@web65710.mail.ac4.yahoo.com> Anthony: Why would you after dehydrating and starting the clearing stage would go back to a dehydrating step. I don't see any rationale in doing that. The thing with fatty tissue is not that they need additional dehydration (since fatty tissue contains less water that other tissues) but they need a complete elimination of fat obtained with the clearing agents. What I used to do was to eliminate the las 2 dehydrating steps, and substitute them with one half:half with dehydrant:clearing followed by an additional clearing and it always worked very well for me. Ren? J. anthony@histotechexchange.com wrote: Dear Ramona: I am late to the game so I am sorry if you have already had this one. I have never done this, so please try it on some fatty tissue that you are going to throw away and secondly I have no idea how it will affect antigen retrieval or anything involving antibodies. With that out of the way you may want to try taking the specimen through alcohols then onto the first clearing agent, then back to alcohol and then back into the clearing agents and on to wax. Good luck on finding the times etc. Can anyone else out there give your opinion on this practice. Yours truly, Anthony Williams BSc. HT Histotech Exchange LLC 19 Whitmore St. Lexington, VA 24450 T 1 (302) 383 9780 F 1 (540) 463 3583 anthony@histotechexchange.com Dear Ramona: I am late to the game so I am sorry if you have already had this one. I have neve done this, so please try it on some lipoma that you are going to throw away and secondly I have no idea how it will affect antigen retreval or anthing involving antibodies. > I also would like some advice on processing fatty tissue. I have tried > using Pen-Fix prior to processing, but my pathologist complain about an > artifact and refuse to use it anymore. Can't explain that one. I am > currently processing with formalin x2, 70% x2, 95% x2, 100% x2, clearing > x2. I thought the dehydration step should be increased on the fatty > tissue, but you are saying that is not the problem? Can someone else > please offer some experience on this issue. > > Ramona Turner, HT (ASCP) > Potomac Hospital > Woodbridge, VA > _________________________________________________________________ > Climb to the top of the charts! Play the word scramble challenge with star > power. > http://club.live.com/star_shuffle.aspx?icid=starshuffle_wlmailtextlink_jan_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From rjbuesa <@t> yahoo.com Wed Mar 12 09:11:03 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 12 09:11:11 2008 Subject: [Histonet] salery survey In-Reply-To: <955992.48865.qm@web36103.mail.mud.yahoo.com> Message-ID: <326455.77544.qm@web65713.mail.ac4.yahoo.com> Under separate cover I am sending you my article (Annals of Diagnostic Pathology) on safety. Ren? J. richard shook wrote: A quick question for all is there, or has there been a recent histology salery survey and if so where could i find it. i have seen one before but cant remember where it was thank you rich rabkin derm path ____________________________________________________________________________________ Looking for last minute shopping deals? Find them fast with Yahoo! Search. http://tools.search.yahoo.com/newsearch/category.php?category=shopping _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From cbobrowi <@t> mcw.edu Wed Mar 12 10:39:57 2008 From: cbobrowi <@t> mcw.edu (Bobrowitz, Carol) Date: Wed Mar 12 10:40:39 2008 Subject: [Histonet] Book for Fluorescent Staining Message-ID: <8F78639AC56F4143B267FE5F5A1B92C8F0AEE0@guyton.phys.mcw.edu> Hi, I am looking for a book or information booklet pertaining strictly to fluorescent staining. Something on the order of Dako' s immunohistochemical staining book or C.M. vander Loos's multiple staining book. Even a web page reference would be helpful. I know Galye Callis is giving a workshop this summer in New Mexico, unfortunately I can not attend. Hopefully she will be giving this workshop in Pittsburg. Any information that you can provide me will be greatly appreciated. Thank you in advance for your help. Carol Ann Bobrowitz Department of Physiology Medical College of Wisconsin 414-456-8179 cbobrowi@mcw.edu From KLAPANO1 <@t> hfhs.org Wed Mar 12 10:16:58 2008 From: KLAPANO1 <@t> hfhs.org (Karen Lapanowski) Date: Wed Mar 12 11:15:05 2008 Subject: [Histonet] Air bubbles Message-ID: <20080312T111658Z_02DE000F0000@hfhs.org> Hello, Air bubbles develop a week or so after coverslipping sections using Vectashield hardset mounting media. (Initially, no air pockets were visible.) Any suggestions? Thanks, Karen ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== From JMacDonald <@t> mtsac.edu Wed Mar 12 11:26:30 2008 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Mar 12 11:26:43 2008 Subject: [Histonet] CSH Symposium Message-ID: The California Society for Histotechnology annual symposium will be held at the Ventura Beach Marriott in Ventura, California. Please visit our website for the complete program. Thank you, http://www.californiahistology.org/ Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu From Terry.Marshall <@t> rothgen.nhs.uk Wed Mar 12 11:31:39 2008 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Wed Mar 12 11:31:18 2008 Subject: [Histonet] Air bubbles Message-ID: <5C0BED61F529364E86309CADEA63FEF20163F387@TRFT-EX01.xRothGen.nhs.uk> We are getting the same problem, except that it is (as yours probably is) "dryback". Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Karen Lapanowski Sent: 12 March 2008 15:17 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Air bubbles Hello, Air bubbles develop a week or so after coverslipping sections using Vectashield hardset mounting media. (Initially, no air pockets were visible.) Any suggestions? Thanks, Karen ======================================================================== ====== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ======================================================================== ====== ###################################################################### This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. ###################################################################### From histion <@t> hotmail.com Wed Mar 12 11:32:07 2008 From: histion <@t> hotmail.com (Simon Smith) Date: Wed Mar 12 11:32:27 2008 Subject: [Histonet] HMP300 tissue processor Message-ID: Folks, I have an old but good Microm/Hacker HMP300 tissue processor which is refusing to behave at the moment. When trying to run it fails at diagnostic #3, A more detailed check reveals the problem to be a "Station valve driver fail off" error for station 14 (the xylene cleaning step). I have obtained and replaced the valve to no effect, I swapped the electrical connections between station 14 and 15 (I still get the same error for station 14) and am at the end of my patience with the wee beastie. I have the following questions: 1. What is causing the problem? 2. How do I fix it? 3. Is there anyone out there who remembers these machines and can fix the problem? I am located just North of Seattle. 4. Failing that, does anyone have a service manual and/or parts I can borrow or buy? Thanks in advance for your help Simon From Ronald.Houston <@t> nationwidechildrens.org Wed Mar 12 11:49:40 2008 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Wed Mar 12 11:50:12 2008 Subject: [Histonet] Book for Fluorescent Staining In-Reply-To: <8F78639AC56F4143B267FE5F5A1B92C8F0AEE0@guyton.phys.mcw.edu> Message-ID: <979FF5962E234F45B06CF0DB7C1AABB215E8CF1A@chi2k3ms01.columbuschildrens.net> Protein Localization by Fluorescence Microscopy: a practical approach Ed. VJ Allen Pub OUP, 2000 ISBN: 0-19-963741-5 (hbk) 0-19-963740-7 (pbk) Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 Ronald.Houston@NationwideChildrens.org Columbus Children's Hospital is now Nationwide Children's Hospital www.NationwideChildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bobrowitz, Carol Sent: Wednesday, March 12, 2008 11:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Book for Fluorescent Staining Hi, I am looking for a book or information booklet pertaining strictly to fluorescent staining. Something on the order of Dako' s immunohistochemical staining book or C.M. vander Loos's multiple staining book. Even a web page reference would be helpful. I know Galye Callis is giving a workshop this summer in New Mexico, unfortunately I can not attend. Hopefully she will be giving this workshop in Pittsburg. Any information that you can provide me will be greatly appreciated. Thank you in advance for your help. Carol Ann Bobrowitz Department of Physiology Medical College of Wisconsin 414-456-8179 cbobrowi@mcw.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From crochieresteve <@t> aol.com Wed Mar 12 12:08:25 2008 From: crochieresteve <@t> aol.com (crochieresteve@aol.com) Date: Wed Mar 12 12:08:58 2008 Subject: [Histonet] prostate bx falling off slide Message-ID: <8CA527AD164F369-9A4-11D4@webmail-me06.sysops.aol.com> Are there any suggestions to help keep prostate core biopsies on the plus slides when doing PIN-4 IHC. I use the Biocare de-cloaker and their BORG retrieval solution and Nemesis autostainer. I have been having a tough time with sections falling away after retrieval. I air dry them overnight and incubate in a 60 degree oven for 1 hour prior to de-wax and staining. What to do?? Steve Crochiere Histology Supervisor Life Path Partners, Mercy Medical Center From crochieresteve <@t> aol.com Wed Mar 12 12:09:54 2008 From: crochieresteve <@t> aol.com (crochieresteve@aol.com) Date: Wed Mar 12 12:10:09 2008 Subject: [Histonet] HT position available Message-ID: <8CA527B06CE9B3B-9A4-11E6@webmail-me06.sysops.aol.com> We are looking for an experienced HT in the Springfield MA area to work in our hospital based laboratory and our satellite prostate lab. Send resume to Steve.crochiere@SPHS.com From Jessica.Vacca <@t> HCAhealthcare.com Wed Mar 12 12:52:27 2008 From: Jessica.Vacca <@t> HCAhealthcare.com (Vacca Jessica) Date: Wed Mar 12 12:52:36 2008 Subject: [Histonet] Microwaves to keep or not to keep Message-ID: <41E16A15CE78374EA45B57E0F94339B8037DDCC8@ORLEV01.hca.corpad.net> I would like to know how many of you are keeping your "household" microwaves? I do a couple of special stains that we heat up the solutions. We don't do any processing with it. Those of you that are keeping them, what are you doing to avoid the ding? Or are you just taking your chances? Which do you recommend for a "lab" microwave? Thanks Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr. Brandon Fl 33511 (813) 571-5193 or (813) 681-5551 ext 2454 Jessica.Vacca@hcahealthcare.com From ktuttle <@t> umm.edu Wed Mar 12 12:57:58 2008 From: ktuttle <@t> umm.edu (Kimberly Tuttle) Date: Wed Mar 12 12:58:20 2008 Subject: [Histonet] S100P Message-ID: <47D7E165.90CE.001A.3@umm.edu> Does anyone know where I can buy S100P, how much it costs, lead time.....Thanks Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From Jenee.S.Odani <@t> hawaii.gov Wed Mar 12 13:10:57 2008 From: Jenee.S.Odani <@t> hawaii.gov (Jenee.S.Odani@hawaii.gov) Date: Wed Mar 12 13:11:10 2008 Subject: [Histonet] Good Lab Practices (clarification) Message-ID: I posted yesterday asking if anyone would share their protocols/manuals regarding disposal of blocks/tissues. For clarification, this is not because I need info to write a manual. Our state does not have any clear guidelines, and a USDA official is trying to get an idea of "what other labs do" to help certify a new lab in our state. Can you share with me how your lab disposes of blocks and tissues? What logs or records are kept? Jenee S. Odani, D.V.M., Dipl. ACVP Veterinary Medical Officer Hawaii State Veterinary Laboratory/DAI 99-941 Halawa Valley Street, Aiea, HI, 96701 Phone: (808) 483-7131/Fax: (808) 483-7110 From DEllenburg2 <@t> stfrancishealth.org Wed Mar 12 13:27:59 2008 From: DEllenburg2 <@t> stfrancishealth.org (Ellenburg, Deborah) Date: Wed Mar 12 13:28:13 2008 Subject: [Histonet] RE: Salary Survey for 2007-2008 In-Reply-To: Message-ID: Advance for Medical Laboratory Professionals is doing an online salary survey and are to have the results of the survey available in June. You can logo to www.advanceweb.com/surveys to participate in the survey. Hope this helps. Debbie Ellenburg, HT (ASCP) Histology Supervisor Bon Secours St. Francis Health System One St. Francis Drive Greenville, SC 29601 864-255-1582 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, March 12, 2008 1:10 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 52, Issue 18 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: salery survey (Rene J Buesa) 2. Book for Fluorescent Staining (Bobrowitz, Carol) 3. Air bubbles (Karen Lapanowski) 4. CSH Symposium (Jennifer MacDonald) 5. RE: Air bubbles (Marshall Terry Dr, Consultant Histopathologist) 6. HMP300 tissue processor (Simon Smith) 7. RE: Book for Fluorescent Staining (Houston, Ronald) ---------------------------------------------------------------------- Message: 1 Date: Wed, 12 Mar 2008 07:11:03 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] salery survey To: richard shook , histonet@lists.utsouthwestern.edu Message-ID: <326455.77544.qm@web65713.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Under separate cover I am sending you my article (Annals of Diagnostic Pathology) on safety. Ren? J. richard shook wrote: A quick question for all is there, or has there been a recent histology salery survey and if so where could i find it. i have seen one before but cant remember where it was thank you rich rabkin derm path ____________________________________________________________________________________ Looking for last minute shopping deals? Find them fast with Yahoo! Search. http://tools.search.yahoo.com/newsearch/category.php?category=shopping _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. ------------------------------ Message: 2 Date: Wed, 12 Mar 2008 10:39:57 -0500 From: "Bobrowitz, Carol" Subject: [Histonet] Book for Fluorescent Staining To: Message-ID: <8F78639AC56F4143B267FE5F5A1B92C8F0AEE0@guyton.phys.mcw.edu> Content-Type: text/plain; charset="us-ascii" Hi, I am looking for a book or information booklet pertaining strictly to fluorescent staining. Something on the order of Dako' s immunohistochemical staining book or C.M. vander Loos's multiple staining book. Even a web page reference would be helpful. I know Galye Callis is giving a workshop this summer in New Mexico, unfortunately I can not attend. Hopefully she will be giving this workshop in Pittsburg. Any information that you can provide me will be greatly appreciated. Thank you in advance for your help. Carol Ann Bobrowitz Department of Physiology Medical College of Wisconsin 414-456-8179 cbobrowi@mcw.edu ------------------------------ Message: 3 Date: Wed, 12 Mar 2008 11:16:58 -0400 From: "Karen Lapanowski" Subject: [Histonet] Air bubbles To: histonet@lists.utsouthwestern.edu Message-ID: <20080312T111658Z_02DE000F0000@hfhs.org> Content-Type: text/plain; charset=utf-8 Hello, Air bubbles develop a week or so after coverslipping sections using Vectashield hardset mounting media. (Initially, no air pockets were visible.) Any suggestions? Thanks, Karen ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== ------------------------------ Message: 4 Date: Wed, 12 Mar 2008 09:26:30 -0700 From: Jennifer MacDonald Subject: [Histonet] CSH Symposium To: histonet@lists.utsouthwestern.edu Cc: lfigueroa@sakuraus.com Message-ID: Content-Type: text/plain; charset="US-ASCII" The California Society for Histotechnology annual symposium will be held at the Ventura Beach Marriott in Ventura, California. Please visit our website for the complete program. Thank you, http://www.californiahistology.org/ Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu ------------------------------ Message: 5 Date: Wed, 12 Mar 2008 16:31:39 -0000 From: "Marshall Terry Dr, Consultant Histopathologist" Subject: RE: [Histonet] Air bubbles To: "Karen Lapanowski" , Message-ID: <5C0BED61F529364E86309CADEA63FEF20163F387@TRFT-EX01.xRothGen.nhs.uk> Content-Type: text/plain; charset="us-ascii" We are getting the same problem, except that it is (as yours probably is) "dryback". Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Karen Lapanowski Sent: 12 March 2008 15:17 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Air bubbles Hello, Air bubbles develop a week or so after coverslipping sections using Vectashield hardset mounting media. (Initially, no air pockets were visible.) Any suggestions? Thanks, Karen ======================================================================== ====== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ======================================================================== ====== ###################################################################### This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. ###################################################################### ------------------------------ Message: 6 Date: Wed, 12 Mar 2008 09:32:07 -0700 From: "Simon Smith" Subject: [Histonet] HMP300 tissue processor To: Message-ID: Content-Type: text/plain; charset="us-ascii" Folks, I have an old but good Microm/Hacker HMP300 tissue processor which is refusing to behave at the moment. When trying to run it fails at diagnostic #3, A more detailed check reveals the problem to be a "Station valve driver fail off" error for station 14 (the xylene cleaning step). I have obtained and replaced the valve to no effect, I swapped the electrical connections between station 14 and 15 (I still get the same error for station 14) and am at the end of my patience with the wee beastie. I have the following questions: 1. What is causing the problem? 2. How do I fix it? 3. Is there anyone out there who remembers these machines and can fix the problem? I am located just North of Seattle. 4. Failing that, does anyone have a service manual and/or parts I can borrow or buy? Thanks in advance for your help Simon ------------------------------ Message: 7 Date: Wed, 12 Mar 2008 12:49:40 -0400 From: "Houston, Ronald" Subject: RE: [Histonet] Book for Fluorescent Staining To: "Bobrowitz, Carol" , Message-ID: <979FF5962E234F45B06CF0DB7C1AABB215E8CF1A@chi2k3ms01.columbuschildrens.net> Content-Type: text/plain; charset="us-ascii" Protein Localization by Fluorescence Microscopy: a practical approach Ed. VJ Allen Pub OUP, 2000 ISBN: 0-19-963741-5 (hbk) 0-19-963740-7 (pbk) Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 Ronald.Houston@NationwideChildrens.org Columbus Children's Hospital is now Nationwide Children's Hospital www.NationwideChildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bobrowitz, Carol Sent: Wednesday, March 12, 2008 11:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Book for Fluorescent Staining Hi, I am looking for a book or information booklet pertaining strictly to fluorescent staining. Something on the order of Dako' s immunohistochemical staining book or C.M. vander Loos's multiple staining book. Even a web page reference would be helpful. I know Galye Callis is giving a workshop this summer in New Mexico, unfortunately I can not attend. Hopefully she will be giving this workshop in Pittsburg. Any information that you can provide me will be greatly appreciated. Thank you in advance for your help. Carol Ann Bobrowitz Department of Physiology Medical College of Wisconsin 414-456-8179 cbobrowi@mcw.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 52, Issue 18 **************************************** ________________________________________________________________________________________________________________________________ ________________________________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. From rjbuesa <@t> yahoo.com Wed Mar 12 14:38:11 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 12 14:38:18 2008 Subject: [Histonet] Good Lab Practices (clarification) In-Reply-To: Message-ID: <200124.30165.qm@web65713.mail.ac4.yahoo.com> We used to incinerate them after keeping them for the prescribed time period. Ren? J. Jenee.S.Odani@hawaii.gov wrote: I posted yesterday asking if anyone would share their protocols/manuals regarding disposal of blocks/tissues. For clarification, this is not because I need info to write a manual. Our state does not have any clear guidelines, and a USDA official is trying to get an idea of "what other labs do" to help certify a new lab in our state. Can you share with me how your lab disposes of blocks and tissues? What logs or records are kept? Jenee S. Odani, D.V.M., Dipl. ACVP Veterinary Medical Officer Hawaii State Veterinary Laboratory/DAI 99-941 Halawa Valley Street, Aiea, HI, 96701 Phone: (808) 483-7131/Fax: (808) 483-7110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From rjbuesa <@t> yahoo.com Wed Mar 12 14:42:54 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 12 14:43:06 2008 Subject: [Histonet] prostate bx falling off slide In-Reply-To: <8CA527AD164F369-9A4-11D4@webmail-me06.sysops.aol.com> Message-ID: <307439.49289.qm@web65706.mail.ac4.yahoo.com> Check your processing protocol. Maybe they are treated too long in dehydrant/clearant. How do they section, brittle? If that is the case it is an indicaiton of a too prolonged processing protocol. Ren? J. crochieresteve@aol.com wrote: Are there any suggestions to help keep prostate core biopsies on the plus slides when doing PIN-4 IHC. I use the Biocare de-cloaker and their BORG retrieval solution and Nemesis autostainer. I have been having a tough time with sections falling away after retrieval. I air dry them overnight and incubate in a 60 degree oven for 1 hour prior to de-wax and staining. What to do?? Steve Crochiere Histology Supervisor Life Path Partners, Mercy Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From KLAPANO1 <@t> hfhs.org Wed Mar 12 15:24:40 2008 From: KLAPANO1 <@t> hfhs.org (Karen Lapanowski) Date: Wed Mar 12 15:25:05 2008 Subject: [Histonet] Doublecortin Message-ID: <20080312T162440Z_02DE000F0000@hfhs.org> Hello, What supplier do you use for doublecortin antibody? Will be staining paraffin sections using FITC as the secondary. Thanks, Karen Lapanowski Karen Lapanowski Radiation Oncology 3065 E & R 313-916-9386 ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== From celebrej <@t> HHSC.CA Wed Mar 12 15:41:13 2008 From: celebrej <@t> HHSC.CA (Celebre Julia) Date: Wed Mar 12 15:41:23 2008 Subject: [Histonet] Textbook referrals Message-ID: <4D3667D4B5487546A3139FB918181FEA078E34@ipemail01.hhsc.ca> Believe it or not, but there are funds available to us for purchasing new Histology textbooks. I'm wondering if anyone in Histoland could kindly suggest some good textbooks that are now available. Julia Celebre MLT Anatomic Pathology Hamilton General Hospital 905-527-0271 ext 46179 email: celebrej@hhsc.ca This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. From pvalente <@t> sbcglobal.net Wed Mar 12 15:47:41 2008 From: pvalente <@t> sbcglobal.net (Patricia Valente) Date: Wed Mar 12 15:48:10 2008 Subject: [Histonet] prostate bx falling off slide In-Reply-To: <8CA527AD164F369-9A4-11D4@webmail-me06.sysops.aol.com> Message-ID: <447310.77002.qm@web81703.mail.mud.yahoo.com> After dewaxing the slides and taking down to water try 10 minutes in 10%NBF, rinse well before retrieval. Pat valente uropathllc --- crochieresteve@aol.com wrote: > Are there any suggestions to help keep prostate core > biopsies on the plus slides when doing PIN-4 IHC. I > use the Biocare de-cloaker and their BORG retrieval > solution and Nemesis autostainer. I have been having > a tough time with sections falling away after > retrieval. I air dry them overnight and incubate in > a 60 degree oven for 1 hour prior to de-wax and > staining. What to do?? > Steve Crochiere > Histology Supervisor > Life Path Partners, Mercy Medical Center > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Pat From langston <@t> hawaii.edu Wed Mar 12 15:55:53 2008 From: langston <@t> hawaii.edu (Ross Christian Langston) Date: Wed Mar 12 15:56:03 2008 Subject: [Histonet] Digital Microscope Cameras for Classroom Use In-Reply-To: <20080312T162440Z_02DE000F0000@hfhs.org> References: <20080312T162440Z_02DE000F0000@hfhs.org> Message-ID: Hi Folks- could anyone with experience with either of the following two microscope cameras weigh-in on their merits or drawbacks? We are planning on projecting the feed onto a screen in our anatomy and physiology and lab techniques classes. Thanks in advance for your input! Ross The cameras: Q imaging Micropublisher 5.0 (uncooled) Lumenera Infinity 1-1 Ross Langston, PhD Department of Natural Sciences Windward Community College 45-720 Keaahala Road Kaneohe, Hawaii 96744 808-236-9119 (office) 808-247-5362 (fax) ----- Original Message ----- From: Karen Lapanowski Date: Wednesday, March 12, 2008 10:26 am Subject: [Histonet] Doublecortin To: histonet@lists.utsouthwestern.edu > Hello, > What supplier do you use for doublecortin antibody? Will be > staining paraffin sections using FITC as the secondary. > Thanks, > Karen Lapanowski > > Karen Lapanowski > Radiation Oncology > 3065 E & R > 313-916-9386 > ============================================================================== > CONFIDENTIALITY NOTICE: This email contains information from the > sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY > or otherwise protected from disclosure. This email is intended for > use only by the person or entity to whom it is addressed. If you > are not the intended recipient, any use, disclosure, copying, > distribution, printing, or any action taken in reliance on the > contents of this email, is strictly prohibited. If you received > this email in error, please contact the sending party by reply > email, delete the email from your computer system and shred any > paper copies. > > Note to Patients: There are a number of risks you should consider > before using e-mail to communicate with us. See our Privacy Policy > and Henry Ford My Health at www.henryford.com for more detailed > information. If you do not believe that our policy gives you the > privacy and security protection you need, do not send e-mail or > Internet communications to us. > > ============================================================================== > From bob.nienhuis <@t> gmail.com Wed Mar 12 17:47:47 2008 From: bob.nienhuis <@t> gmail.com (Bob Nienhuis) Date: Wed Mar 12 17:47:52 2008 Subject: [Histonet] MMA for Golgi? Message-ID: <45109da50803121547w1c69afb5k64fb11c61c61381b@mail.gmail.com> Someone suggested the possibility of using MMA as an embedding medium instead of celloidin for rapid Golgi. How is it made soft enough for cutting with a microtome? Anyone have experience with this, or have a protocol for doing this? Any thoughts or comments? I have looked at the Neil Hand papers, but they don't seen to exactly apply to what I want to do. I am looking to do thick sections so as to see full dendritic arborization. Bob Nienhuis UCLA / VA Medical Center Neurobiology Research North Hills, CA 91343 From lpwenk <@t> sbcglobal.net Thu Mar 13 04:37:53 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Mar 13 04:38:19 2008 Subject: [Histonet] Textbook referrals In-Reply-To: <4D3667D4B5487546A3139FB918181FEA078E34@ipemail01.hhsc.ca> Message-ID: <000301c884ed$f1ab4c70$0202a8c0@HPPav2> NSH website has a list of books that are available and current, and were suggested by Histonet members. www.nsh.org Highlight Career Center on left Click on Tools and Resources Click on Reference Materials Books listed by topic: - Histotechnology - Management/Education - Immunohistochemistry - Molecular Pathology - Histology Atlas - Lab Math - Lab Safety - Misc. (skin, cytoprep) Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Celebre Julia Sent: Wednesday, March 12, 2008 4:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Textbook referrals Believe it or not, but there are funds available to us for purchasing new Histology textbooks. I'm wondering if anyone in Histoland could kindly suggest some good textbooks that are now available. Julia Celebre MLT Anatomic Pathology Hamilton General Hospital 905-527-0271 ext 46179 email: celebrej@hhsc.ca This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From olek.michalski <@t> nencki.gov.pl Thu Mar 13 07:34:10 2008 From: olek.michalski <@t> nencki.gov.pl (Olek Michalski) Date: Thu Mar 13 07:27:27 2008 Subject: [Histonet] Doublecortin In-Reply-To: <20080312T162440Z_02DE000F0000@hfhs.org> References: <20080312T162440Z_02DE000F0000@hfhs.org> Message-ID: I use Santa Cruz Biochemicals and the antibody (produced in goat) works perfectly. Best Regards Olek Michalski 2008-03-12 21:24:40 Karen Lapanowski wrote: > Hello, > What supplier do you use for doublecortin antibody? Will be staining > paraffin sections using FITC as the secondary. > Thanks, > Karen Lapanowski > > Karen Lapanowski > Radiation Oncology > 3065 E & R > 313-916-9386 > ============================================================================== > CONFIDENTIALITY NOTICE: This email contains information from the sender > that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise > protected from disclosure. This email is intended for use only by the > person or entity to whom it is addressed. If you are not the intended > recipient, any use, disclosure, copying, distribution, printing, or any > action taken in reliance on the contents of this email, is strictly > prohibited. If you received this email in error, please contact the > sending party by reply email, delete the email from your computer system > and shred any paper copies. > Note to Patients: There are a number of risks you should consider before > using e-mail to communicate with us. See our Privacy Policy and Henry > Ford My Health at www.henryford.com for more detailed information. If > you do not believe that our policy gives you the privacy and security > protection you need, do not send e-mail or Internet communications to us. > > ============================================================================== -- Laboratory of Neurobiology of Development and Evolution Nencki Institute of Experimental Biology ul. Pasteura 3, 02-093 Warszawa, Poland Tel. +48 22 5892268, Fax +48 22 8225342 From mlm11 <@t> cornell.edu Thu Mar 13 08:09:00 2008 From: mlm11 <@t> cornell.edu (Mary Lou Norman) Date: Thu Mar 13 08:05:39 2008 Subject: [Histonet] What type media? Message-ID: <6.2.1.2.2.20080313085627.03c0a720@postoffice9.mail.cornell.edu> Hello, I don't do immuno so I am clueless. Please tell me the best media to grow lung cells on so I can prepare them later for paraffin histopath. H&E. Thank you, Mary Lou From asmith <@t> mail.barry.edu Thu Mar 13 08:07:27 2008 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Thu Mar 13 08:08:13 2008 Subject: [Histonet] RE: Textbook referrals In-Reply-To: <4D3667D4B5487546A3139FB918181FEA078E34@ipemail01.hhsc.ca> Message-ID: HISTOLOGY: A TEXT AND ATLAS, 5th ed. by Michael Ross and Wojciech Pawlina (long and detailed) WHEATER'S FUNCTIONAL HISTOLOGY, 4th ed. by Barbara Young (very concise) Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Celebre Julia Sent: Wednesday, March 12, 2008 3:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Textbook referrals Believe it or not, but there are funds available to us for purchasing new Histology textbooks. I'm wondering if anyone in Histoland could kindly suggest some good textbooks that are now available. Julia Celebre MLT Anatomic Pathology Hamilton General Hospital 905-527-0271 ext 46179 email: celebrej@hhsc.ca This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Thu Mar 13 08:51:39 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Mar 13 08:51:48 2008 Subject: [Histonet] Air bubbles In-Reply-To: <5C0BED61F529364E86309CADEA63FEF20163F387@TRFT-EX01.xRothGen.nhs.uk> References: <5C0BED61F529364E86309CADEA63FEF20163F387@TRFT-EX01.xRothGen.nhs.uk> Message-ID: This may sound kind of stupid, but I always thought it might be because more mounting media was needed when coverslipping. A lab down the hall used the same protocol as ours and always had air bubbles after a week. They only used two drops of media for coverslipping, but we use four to five. Emily -- People aren't like chocolates. People are bastards. Bastards with bastard coating and bastard filling. From vazquezr <@t> ohsu.edu Thu Mar 13 09:04:02 2008 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Thu Mar 13 09:04:40 2008 Subject: [Histonet] Gills III Message-ID: Hello, We have been using Gills III, as of recently approx 1 month, to do frozens on a linear stainer, it has been getting stringy. Not a lot but significantly. We rec'd it in 6/07 and opened it in 9/07. Could it be too old? I tried filtering it, but still stringy. I also, turned up the water to help rinse it, but I don't want to take a chance on losing tissue. Thanks in advanced. Robyn OHSU From hej01 <@t> health.state.ny.us Thu Mar 13 09:09:32 2008 From: hej01 <@t> health.state.ny.us (Helen E Johnson) Date: Thu Mar 13 09:09:37 2008 Subject: [Histonet] marker resistant to triton Message-ID: Hi Histonetters, I am inquiring if there is a permanent slide marker that is resistant to the chemical triton when doing IHC. Helen Johnson (hej01@health.state.ny.us) IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. From c.weaver <@t> vla.defra.gsi.gov.uk Thu Mar 13 10:00:49 2008 From: c.weaver <@t> vla.defra.gsi.gov.uk (Weaver, Colin) Date: Thu Mar 13 10:01:05 2008 Subject: [Histonet] Lung processing Message-ID: <7A885E8FE1C71C488D974EC601FAA69002E0865A@vla-exchn1.cvlnt.vla.gov.uk> Hi - everyone we have just moved from routine processing in a VIP 1000 to a Thermo-Shandon Excelsior. This has been working perfectly except when processing lung, especially spongy lung. When embedding after processing wax infiltration appears to be patchy, bubbles come out of the tissue when pressing the tissue into a mould, after trimming there are "holes" in the tissue, and sections often crumble because of lack of wax support. We have vacuumn on everything from first alcohol onwards. Anyone got any suggestions as to why this is happening? We used to use Thermo-Shandon Hypercenter XP's and had the same problem with lung, is it a Thermo thing? Colin Histology Dept VLA Thirsk Sunny Yorkshire Veterinary Laboratories Agency (VLA) This email and any attachments is intended for the named recipient only. If you have received it in error you have no authority to use, disclose, store or copy any of its contents and you should destroy it and inform the sender. Whilst this email and associated attachments will have been checked for known viruses whilst within VLA systems we can accept no responsibility once it has left our systems. Communications on VLA's computer systems may be monitored and/or recorded to secure the effective operation of the system and for other lawful purposes. From rjbuesa <@t> yahoo.com Thu Mar 13 10:24:09 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 13 10:24:13 2008 Subject: [Histonet] Air bubbles In-Reply-To: Message-ID: <807810.16869.qm@web65703.mail.ac4.yahoo.com> Emily: That does not sound stupid, less mounting medium than required cause bubbles formation, as well as too tick mounting medium. Ren? J. Emily Sours wrote: This may sound kind of stupid, but I always thought it might be because more mounting media was needed when coverslipping. A lab down the hall used the same protocol as ours and always had air bubbles after a week. They only used two drops of media for coverslipping, but we use four to five. Emily -- People aren't like chocolates. People are bastards. Bastards with bastard coating and bastard filling. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From rjbuesa <@t> yahoo.com Thu Mar 13 10:26:24 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 13 10:26:30 2008 Subject: [Histonet] Gills III In-Reply-To: Message-ID: <605838.80002.qm@web65716.mail.ac4.yahoo.com> Opening a hematoxylin 3 months after receiving it cannot be the cause UNLESS it has an expiration date within that period, or is close to expiring (lets say before 2008). Ren? J. Robyn Vazquez wrote: Hello, We have been using Gills III, as of recently approx 1 month, to do frozens on a linear stainer, it has been getting stringy. Not a lot but significantly. We rec'd it in 6/07 and opened it in 9/07. Could it be too old? I tried filtering it, but still stringy. I also, turned up the water to help rinse it, but I don't want to take a chance on losing tissue. Thanks in advanced. Robyn OHSU _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From rjbuesa <@t> yahoo.com Thu Mar 13 11:09:48 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 13 11:09:56 2008 Subject: [Histonet] Lung processing In-Reply-To: <7A885E8FE1C71C488D974EC601FAA69002E0865A@vla-exchn1.cvlnt.vla.gov.uk> Message-ID: <380780.53437.qm@web65703.mail.ac4.yahoo.com> Colin: You hit "the nail in the head". It is "vacuum thing", I don't know if it is a "Thermo thing". Check their operating manual and find out the vacuum level (mm of Hg) and compare with what the VIP 1000 used to have. If the vacuum is higher in the VIP 1000, that is your answer, if it is the same, then it has to be a "Thermo thing". Ren? J. "Weaver, Colin" wrote: Hi - everyone we have just moved from routine processing in a VIP 1000 to a Thermo-Shandon Excelsior. This has been working perfectly except when processing lung, especially spongy lung. When embedding after processing wax infiltration appears to be patchy, bubbles come out of the tissue when pressing the tissue into a mould, after trimming there are "holes" in the tissue, and sections often crumble because of lack of wax support. We have vacuumn on everything from first alcohol onwards. Anyone got any suggestions as to why this is happening? We used to use Thermo-Shandon Hypercenter XP's and had the same problem with lung, is it a Thermo thing? Colin Histology Dept VLA Thirsk Sunny Yorkshire Veterinary Laboratories Agency (VLA) This email and any attachments is intended for the named recipient only. If you have received it in error you have no authority to use, disclose, store or copy any of its contents and you should destroy it and inform the sender. Whilst this email and associated attachments will have been checked for known viruses whilst within VLA systems we can accept no responsibility once it has left our systems. Communications on VLA's computer systems may be monitored and/or recorded to secure the effective operation of the system and for other lawful purposes. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From Kristopher.Kalleberg <@t> unilever.com Thu Mar 13 11:16:01 2008 From: Kristopher.Kalleberg <@t> unilever.com (Kalleberg, Kristopher) Date: Thu Mar 13 11:16:11 2008 Subject: [Histonet] microtome calibration Message-ID: <0E6BC087F70F9C47ACFF2C203D6E329C03CBFE33@NTRSEVS30002.s3.ms.unilever.com> Does anyone know of anyone company that calibrates microtomes? I currently have an old Leitz 1512 microtome and I was just recently informed by leica that they no longer service or calibrate these microtomes. If anyone knows of a company that calibrates this microtome I would appreciate any info. The company would have to be in the Connecticut/ tri-State area. Thanks. Kris Kalleberg Research Scientist Unilever R&D 40 Merritt Blvd. Trumbull, CT 06611 (203) 381-5765 From simmonsca <@t> upmc.edu Thu Mar 13 11:24:27 2008 From: simmonsca <@t> upmc.edu (Simmons, Christopher) Date: Thu Mar 13 11:25:00 2008 Subject: [Histonet] RE: Histonet Digest, Vol 52, Issue 19 In-Reply-To: <200803131614.m2DGEXhh005468@msxgwsnsprd02.isdip.upmc.edu> Message-ID: <88D6F512ADC2CB428B90845D2D1388E303A5C354@1upmc-msx6.acct.upmchs.net> Our Lab archives blocks and slides at Iron Mountain. We keep them forever as we are in the process of making a synoptic database for a cancer registry. Most states require a 10 year hold period, but, that also depends on the state. 10 years is a good span as it rules out many legal possibilities. Chris Lead Technologist UPMC Presbyterian Pittsburgh, PA Chris Simmons Lead Technologist PUH Histology 7-7660 desk 13242 pager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, March 13, 2008 12:15 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 52, Issue 19 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. prostate bx falling off slide (crochieresteve@aol.com) 2. HT position available (crochieresteve@aol.com) 3. Microwaves to keep or not to keep (Vacca Jessica) 4. S100P (Kimberly Tuttle) 5. Good Lab Practices (clarification) (Jenee.S.Odani@hawaii.gov) 6. RE: Salary Survey for 2007-2008 (Ellenburg, Deborah) 7. Re: Good Lab Practices (clarification) (Rene J Buesa) 8. Re: prostate bx falling off slide (Rene J Buesa) 9. Doublecortin (Karen Lapanowski) 10. Textbook referrals (Celebre Julia) 11. Re: prostate bx falling off slide (Patricia Valente) 12. Digital Microscope Cameras for Classroom Use (Ross Christian Langston) 13. MMA for Golgi? (Bob Nienhuis) 14. RE: Textbook referrals (Lee & Peggy Wenk) 15. Re: Doublecortin (Olek Michalski) 16. What type media? (Mary Lou Norman) 17. RE: Textbook referrals (Smith, Allen) 18. Re: Air bubbles (Emily Sours) 19. Gills III (Robyn Vazquez) 20. marker resistant to triton (Helen E Johnson) 21. Lung processing (Weaver, Colin) 22. Re: Air bubbles (Rene J Buesa) 23. Re: Gills III (Rene J Buesa) 24. Re: Lung processing (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Wed, 12 Mar 2008 13:08:25 -0400 From: crochieresteve@aol.com Subject: [Histonet] prostate bx falling off slide To: histonet@pathology.swmed.edu Message-ID: <8CA527AD164F369-9A4-11D4@webmail-me06.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Are there any suggestions to help keep prostate core biopsies on the plus slides when doing PIN-4 IHC. I use the Biocare de-cloaker and their BORG retrieval solution and Nemesis autostainer. I have been having a tough time with sections falling away after retrieval. I air dry them overnight and incubate in a 60 degree oven for 1 hour prior to de-wax and staining. What to do?? Steve Crochiere Histology Supervisor Life Path Partners, Mercy Medical Center ------------------------------ Message: 2 Date: Wed, 12 Mar 2008 13:09:54 -0400 From: crochieresteve@aol.com Subject: [Histonet] HT position available To: histonet@pathology.swmed.edu Message-ID: <8CA527B06CE9B3B-9A4-11E6@webmail-me06.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" We are looking for an experienced HT in the Springfield MA area to work in our hospital based laboratory and our satellite prostate lab. Send resume to Steve.crochiere@SPHS.com ------------------------------ Message: 3 Date: Wed, 12 Mar 2008 13:52:27 -0400 From: "Vacca Jessica" Subject: [Histonet] Microwaves to keep or not to keep To: Message-ID: <41E16A15CE78374EA45B57E0F94339B8037DDCC8@ORLEV01.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" I would like to know how many of you are keeping your "household" microwaves? I do a couple of special stains that we heat up the solutions. We don't do any processing with it. Those of you that are keeping them, what are you doing to avoid the ding? Or are you just taking your chances? Which do you recommend for a "lab" microwave? Thanks Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr. Brandon Fl 33511 (813) 571-5193 or (813) 681-5551 ext 2454 Jessica.Vacca@hcahealthcare.com ------------------------------ Message: 4 Date: Wed, 12 Mar 2008 13:57:58 -0400 From: "Kimberly Tuttle" Subject: [Histonet] S100P To: Message-ID: <47D7E165.90CE.001A.3@umm.edu> Content-Type: text/plain; charset=US-ASCII Does anyone know where I can buy S100P, how much it costs, lead time.....Thanks Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. ------------------------------ Message: 5 Date: Wed, 12 Mar 2008 08:10:57 -1000 From: Jenee.S.Odani@hawaii.gov Subject: [Histonet] Good Lab Practices (clarification) To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" I posted yesterday asking if anyone would share their protocols/manuals regarding disposal of blocks/tissues. For clarification, this is not because I need info to write a manual. Our state does not have any clear guidelines, and a USDA official is trying to get an idea of "what other labs do" to help certify a new lab in our state. Can you share with me how your lab disposes of blocks and tissues? What logs or records are kept? Jenee S. Odani, D.V.M., Dipl. ACVP Veterinary Medical Officer Hawaii State Veterinary Laboratory/DAI 99-941 Halawa Valley Street, Aiea, HI, 96701 Phone: (808) 483-7131/Fax: (808) 483-7110 ------------------------------ Message: 6 Date: Wed, 12 Mar 2008 14:27:59 -0400 From: "Ellenburg, Deborah" Subject: [Histonet] RE: Salary Survey for 2007-2008 To: Message-ID: Content-Type: text/plain; charset=iso-8859-1 Advance for Medical Laboratory Professionals is doing an online salary survey and are to have the results of the survey available in June. You can logo to www.advanceweb.com/surveys to participate in the survey. Hope this helps. Debbie Ellenburg, HT (ASCP) Histology Supervisor Bon Secours St. Francis Health System One St. Francis Drive Greenville, SC 29601 864-255-1582 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, March 12, 2008 1:10 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 52, Issue 18 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: salery survey (Rene J Buesa) 2. Book for Fluorescent Staining (Bobrowitz, Carol) 3. Air bubbles (Karen Lapanowski) 4. CSH Symposium (Jennifer MacDonald) 5. RE: Air bubbles (Marshall Terry Dr, Consultant Histopathologist) 6. HMP300 tissue processor (Simon Smith) 7. RE: Book for Fluorescent Staining (Houston, Ronald) ---------------------------------------------------------------------- Message: 1 Date: Wed, 12 Mar 2008 07:11:03 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] salery survey To: richard shook , histonet@lists.utsouthwestern.edu Message-ID: <326455.77544.qm@web65713.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Under separate cover I am sending you my article (Annals of Diagnostic Pathology) on safety. Ren? J. richard shook wrote: A quick question for all is there, or has there been a recent histology salery survey and if so where could i find it. i have seen one before but cant remember where it was thank you rich rabkin derm path ____________________________________________________________________________________ Looking for last minute shopping deals? Find them fast with Yahoo! Search. http://tools.search.yahoo.com/newsearch/category.php?category=shopping _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. ------------------------------ Message: 2 Date: Wed, 12 Mar 2008 10:39:57 -0500 From: "Bobrowitz, Carol" Subject: [Histonet] Book for Fluorescent Staining To: Message-ID: <8F78639AC56F4143B267FE5F5A1B92C8F0AEE0@guyton.phys.mcw.edu> Content-Type: text/plain; charset="us-ascii" Hi, I am looking for a book or information booklet pertaining strictly to fluorescent staining. Something on the order of Dako' s immunohistochemical staining book or C.M. vander Loos's multiple staining book. Even a web page reference would be helpful. I know Galye Callis is giving a workshop this summer in New Mexico, unfortunately I can not attend. Hopefully she will be giving this workshop in Pittsburg. Any information that you can provide me will be greatly appreciated. Thank you in advance for your help. Carol Ann Bobrowitz Department of Physiology Medical College of Wisconsin 414-456-8179 cbobrowi@mcw.edu ------------------------------ Message: 3 Date: Wed, 12 Mar 2008 11:16:58 -0400 From: "Karen Lapanowski" Subject: [Histonet] Air bubbles To: histonet@lists.utsouthwestern.edu Message-ID: <20080312T111658Z_02DE000F0000@hfhs.org> Content-Type: text/plain; charset=utf-8 Hello, Air bubbles develop a week or so after coverslipping sections using Vectashield hardset mounting media. (Initially, no air pockets were visible.) Any suggestions? Thanks, Karen ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== ------------------------------ Message: 4 Date: Wed, 12 Mar 2008 09:26:30 -0700 From: Jennifer MacDonald Subject: [Histonet] CSH Symposium To: histonet@lists.utsouthwestern.edu Cc: lfigueroa@sakuraus.com Message-ID: Content-Type: text/plain; charset="US-ASCII" The California Society for Histotechnology annual symposium will be held at the Ventura Beach Marriott in Ventura, California. Please visit our website for the complete program. Thank you, http://www.californiahistology.org/ Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu ------------------------------ Message: 5 Date: Wed, 12 Mar 2008 16:31:39 -0000 From: "Marshall Terry Dr, Consultant Histopathologist" Subject: RE: [Histonet] Air bubbles To: "Karen Lapanowski" , Message-ID: <5C0BED61F529364E86309CADEA63FEF20163F387@TRFT-EX01.xRothGen.nhs.uk> Content-Type: text/plain; charset="us-ascii" We are getting the same problem, except that it is (as yours probably is) "dryback". Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Karen Lapanowski Sent: 12 March 2008 15:17 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Air bubbles Hello, Air bubbles develop a week or so after coverslipping sections using Vectashield hardset mounting media. (Initially, no air pockets were visible.) Any suggestions? Thanks, Karen ======================================================================== ====== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ======================================================================== ====== ###################################################################### This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. ###################################################################### ------------------------------ Message: 6 Date: Wed, 12 Mar 2008 09:32:07 -0700 From: "Simon Smith" Subject: [Histonet] HMP300 tissue processor To: Message-ID: Content-Type: text/plain; charset="us-ascii" Folks, I have an old but good Microm/Hacker HMP300 tissue processor which is refusing to behave at the moment. When trying to run it fails at diagnostic #3, A more detailed check reveals the problem to be a "Station valve driver fail off" error for station 14 (the xylene cleaning step). I have obtained and replaced the valve to no effect, I swapped the electrical connections between station 14 and 15 (I still get the same error for station 14) and am at the end of my patience with the wee beastie. I have the following questions: 1. What is causing the problem? 2. How do I fix it? 3. Is there anyone out there who remembers these machines and can fix the problem? I am located just North of Seattle. 4. Failing that, does anyone have a service manual and/or parts I can borrow or buy? Thanks in advance for your help Simon ------------------------------ Message: 7 Date: Wed, 12 Mar 2008 12:49:40 -0400 From: "Houston, Ronald" Subject: RE: [Histonet] Book for Fluorescent Staining To: "Bobrowitz, Carol" , Message-ID: <979FF5962E234F45B06CF0DB7C1AABB215E8CF1A@chi2k3ms01.columbuschildrens.net> Content-Type: text/plain; charset="us-ascii" Protein Localization by Fluorescence Microscopy: a practical approach Ed. VJ Allen Pub OUP, 2000 ISBN: 0-19-963741-5 (hbk) 0-19-963740-7 (pbk) Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 Ronald.Houston@NationwideChildrens.org Columbus Children's Hospital is now Nationwide Children's Hospital www.NationwideChildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bobrowitz, Carol Sent: Wednesday, March 12, 2008 11:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Book for Fluorescent Staining Hi, I am looking for a book or information booklet pertaining strictly to fluorescent staining. Something on the order of Dako' s immunohistochemical staining book or C.M. vander Loos's multiple staining book. Even a web page reference would be helpful. I know Galye Callis is giving a workshop this summer in New Mexico, unfortunately I can not attend. Hopefully she will be giving this workshop in Pittsburg. Any information that you can provide me will be greatly appreciated. Thank you in advance for your help. Carol Ann Bobrowitz Department of Physiology Medical College of Wisconsin 414-456-8179 cbobrowi@mcw.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. 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Thank you. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 52, Issue 18 **************************************** ________________________________________________________________________________________________________________________________ ________________________________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ------------------------------ Message: 7 Date: Wed, 12 Mar 2008 12:38:11 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Good Lab Practices (clarification) To: Jenee.S.Odani@hawaii.gov, histonet@lists.utsouthwestern.edu Message-ID: <200124.30165.qm@web65713.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 We used to incinerate them after keeping them for the prescribed time period. Ren? J. Jenee.S.Odani@hawaii.gov wrote: I posted yesterday asking if anyone would share their protocols/manuals regarding disposal of blocks/tissues. For clarification, this is not because I need info to write a manual. Our state does not have any clear guidelines, and a USDA official is trying to get an idea of "what other labs do" to help certify a new lab in our state. Can you share with me how your lab disposes of blocks and tissues? What logs or records are kept? Jenee S. Odani, D.V.M., Dipl. ACVP Veterinary Medical Officer Hawaii State Veterinary Laboratory/DAI 99-941 Halawa Valley Street, Aiea, HI, 96701 Phone: (808) 483-7131/Fax: (808) 483-7110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. ------------------------------ Message: 8 Date: Wed, 12 Mar 2008 12:42:54 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] prostate bx falling off slide To: crochieresteve@aol.com, histonet@pathology.swmed.edu Message-ID: <307439.49289.qm@web65706.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Check your processing protocol. Maybe they are treated too long in dehydrant/clearant. How do they section, brittle? If that is the case it is an indicaiton of a too prolonged processing protocol. Ren? J. crochieresteve@aol.com wrote: Are there any suggestions to help keep prostate core biopsies on the plus slides when doing PIN-4 IHC. I use the Biocare de-cloaker and their BORG retrieval solution and Nemesis autostainer. I have been having a tough time with sections falling away after retrieval. I air dry them overnight and incubate in a 60 degree oven for 1 hour prior to de-wax and staining. What to do?? Steve Crochiere Histology Supervisor Life Path Partners, Mercy Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. ------------------------------ Message: 9 Date: Wed, 12 Mar 2008 16:24:40 -0400 From: "Karen Lapanowski" Subject: [Histonet] Doublecortin To: histonet@lists.utsouthwestern.edu Message-ID: <20080312T162440Z_02DE000F0000@hfhs.org> Content-Type: text/plain; charset=utf-8 Hello, What supplier do you use for doublecortin antibody? Will be staining paraffin sections using FITC as the secondary. Thanks, Karen Lapanowski Karen Lapanowski Radiation Oncology 3065 E & R 313-916-9386 ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== ------------------------------ Message: 10 Date: Wed, 12 Mar 2008 16:41:13 -0400 From: "Celebre Julia" Subject: [Histonet] Textbook referrals To: Message-ID: <4D3667D4B5487546A3139FB918181FEA078E34@ipemail01.hhsc.ca> Content-Type: text/plain; charset="iso-8859-1" Believe it or not, but there are funds available to us for purchasing new Histology textbooks. I'm wondering if anyone in Histoland could kindly suggest some good textbooks that are now available. Julia Celebre MLT Anatomic Pathology Hamilton General Hospital 905-527-0271 ext 46179 email: celebrej@hhsc.ca This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. ------------------------------ Message: 11 Date: Wed, 12 Mar 2008 13:47:41 -0700 (PDT) From: Patricia Valente Subject: Re: [Histonet] prostate bx falling off slide To: Histonet@lists.utsouthwestern.edu Message-ID: <447310.77002.qm@web81703.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 After dewaxing the slides and taking down to water try 10 minutes in 10%NBF, rinse well before retrieval. Pat valente uropathllc --- crochieresteve@aol.com wrote: > Are there any suggestions to help keep prostate core > biopsies on the plus slides when doing PIN-4 IHC. I > use the Biocare de-cloaker and their BORG retrieval > solution and Nemesis autostainer. I have been having > a tough time with sections falling away after > retrieval. I air dry them overnight and incubate in > a 60 degree oven for 1 hour prior to de-wax and > staining. What to do?? > Steve Crochiere > Histology Supervisor > Life Path Partners, Mercy Medical Center > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Pat ------------------------------ Message: 12 Date: Wed, 12 Mar 2008 10:55:53 -1000 From: Ross Christian Langston Subject: [Histonet] Digital Microscope Cameras for Classroom Use To: Karen Lapanowski Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Hi Folks- could anyone with experience with either of the following two microscope cameras weigh-in on their merits or drawbacks? We are planning on projecting the feed onto a screen in our anatomy and physiology and lab techniques classes. Thanks in advance for your input! Ross The cameras: Q imaging Micropublisher 5.0 (uncooled) Lumenera Infinity 1-1 Ross Langston, PhD Department of Natural Sciences Windward Community College 45-720 Keaahala Road Kaneohe, Hawaii 96744 808-236-9119 (office) 808-247-5362 (fax) ----- Original Message ----- From: Karen Lapanowski Date: Wednesday, March 12, 2008 10:26 am Subject: [Histonet] Doublecortin To: histonet@lists.utsouthwestern.edu > Hello, > What supplier do you use for doublecortin antibody? Will be > staining paraffin sections using FITC as the secondary. > Thanks, > Karen Lapanowski > > Karen Lapanowski > Radiation Oncology > 3065 E & R > 313-916-9386 > ============================================================================== > CONFIDENTIALITY NOTICE: This email contains information from the > sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY > or otherwise protected from disclosure. This email is intended for > use only by the person or entity to whom it is addressed. If you > are not the intended recipient, any use, disclosure, copying, > distribution, printing, or any action taken in reliance on the > contents of this email, is strictly prohibited. If you received > this email in error, please contact the sending party by reply > email, delete the email from your computer system and shred any > paper copies. > > Note to Patients: There are a number of risks you should consider > before using e-mail to communicate with us. See our Privacy Policy > and Henry Ford My Health at www.henryford.com for more detailed > information. If you do not believe that our policy gives you the > privacy and security protection you need, do not send e-mail or > Internet communications to us. > > ============================================================================== > ------------------------------ Message: 13 Date: Wed, 12 Mar 2008 14:47:47 -0800 From: "Bob Nienhuis" Subject: [Histonet] MMA for Golgi? To: Histonet Message-ID: <45109da50803121547w1c69afb5k64fb11c61c61381b@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Someone suggested the possibility of using MMA as an embedding medium instead of celloidin for rapid Golgi. How is it made soft enough for cutting with a microtome? Anyone have experience with this, or have a protocol for doing this? Any thoughts or comments? I have looked at the Neil Hand papers, but they don't seen to exactly apply to what I want to do. I am looking to do thick sections so as to see full dendritic arborization. Bob Nienhuis UCLA / VA Medical Center Neurobiology Research North Hills, CA 91343 ------------------------------ Message: 14 Date: Thu, 13 Mar 2008 05:37:53 -0400 From: "Lee & Peggy Wenk" Subject: RE: [Histonet] Textbook referrals To: "'Celebre Julia'" , Message-ID: <000301c884ed$f1ab4c70$0202a8c0@HPPav2> Content-Type: text/plain; charset="us-ascii" NSH website has a list of books that are available and current, and were suggested by Histonet members. www.nsh.org Highlight Career Center on left Click on Tools and Resources Click on Reference Materials Books listed by topic: - Histotechnology - Management/Education - Immunohistochemistry - Molecular Pathology - Histology Atlas - Lab Math - Lab Safety - Misc. (skin, cytoprep) Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Celebre Julia Sent: Wednesday, March 12, 2008 4:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Textbook referrals Believe it or not, but there are funds available to us for purchasing new Histology textbooks. I'm wondering if anyone in Histoland could kindly suggest some good textbooks that are now available. Julia Celebre MLT Anatomic Pathology Hamilton General Hospital 905-527-0271 ext 46179 email: celebrej@hhsc.ca This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Thu, 13 Mar 2008 13:34:10 +0100 From: "Olek Michalski" Subject: Re: [Histonet] Doublecortin To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format=flowed; delsp=yes; charset=iso-8859-2 I use Santa Cruz Biochemicals and the antibody (produced in goat) works perfectly. Best Regards Olek Michalski 2008-03-12 21:24:40 Karen Lapanowski wrote: > Hello, > What supplier do you use for doublecortin antibody? Will be staining > paraffin sections using FITC as the secondary. > Thanks, > Karen Lapanowski > > Karen Lapanowski > Radiation Oncology > 3065 E & R > 313-916-9386 > ============================================================================== > CONFIDENTIALITY NOTICE: This email contains information from the sender > that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise > protected from disclosure. This email is intended for use only by the > person or entity to whom it is addressed. If you are not the intended > recipient, any use, disclosure, copying, distribution, printing, or any > action taken in reliance on the contents of this email, is strictly > prohibited. If you received this email in error, please contact the > sending party by reply email, delete the email from your computer system > and shred any paper copies. > Note to Patients: There are a number of risks you should consider before > using e-mail to communicate with us. See our Privacy Policy and Henry > Ford My Health at www.henryford.com for more detailed information. If > you do not believe that our policy gives you the privacy and security > protection you need, do not send e-mail or Internet communications to us. > > ============================================================================== -- Laboratory of Neurobiology of Development and Evolution Nencki Institute of Experimental Biology ul. Pasteura 3, 02-093 Warszawa, Poland Tel. +48 22 5892268, Fax +48 22 8225342 ------------------------------ Message: 16 Date: Thu, 13 Mar 2008 09:09:00 -0400 From: Mary Lou Norman Subject: [Histonet] What type media? To: histonet@lists.utsouthwestern.edu Message-ID: <6.2.1.2.2.20080313085627.03c0a720@postoffice9.mail.cornell.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Hello, I don't do immuno so I am clueless. Please tell me the best media to grow lung cells on so I can prepare them later for paraffin histopath. H&E. Thank you, Mary Lou ------------------------------ Message: 17 Date: Thu, 13 Mar 2008 09:07:27 -0400 From: "Smith, Allen" Subject: [Histonet] RE: Textbook referrals To: 'Celebre Julia' Cc: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" HISTOLOGY: A TEXT AND ATLAS, 5th ed. by Michael Ross and Wojciech Pawlina (long and detailed) WHEATER'S FUNCTIONAL HISTOLOGY, 4th ed. by Barbara Young (very concise) Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Celebre Julia Sent: Wednesday, March 12, 2008 3:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Textbook referrals Believe it or not, but there are funds available to us for purchasing new Histology textbooks. I'm wondering if anyone in Histoland could kindly suggest some good textbooks that are now available. Julia Celebre MLT Anatomic Pathology Hamilton General Hospital 905-527-0271 ext 46179 email: celebrej@hhsc.ca This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Thu, 13 Mar 2008 09:51:39 -0400 From: "Emily Sours" Subject: Re: [Histonet] Air bubbles To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 This may sound kind of stupid, but I always thought it might be because more mounting media was needed when coverslipping. A lab down the hall used the same protocol as ours and always had air bubbles after a week. They only used two drops of media for coverslipping, but we use four to five. Emily -- People aren't like chocolates. People are bastards. Bastards with bastard coating and bastard filling. ------------------------------ Message: 19 Date: Thu, 13 Mar 2008 07:04:02 -0700 From: "Robyn Vazquez" Subject: [Histonet] Gills III To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Hello, We have been using Gills III, as of recently approx 1 month, to do frozens on a linear stainer, it has been getting stringy. Not a lot but significantly. We rec'd it in 6/07 and opened it in 9/07. Could it be too old? I tried filtering it, but still stringy. I also, turned up the water to help rinse it, but I don't want to take a chance on losing tissue. Thanks in advanced. Robyn OHSU ------------------------------ Message: 20 Date: Thu, 13 Mar 2008 10:09:32 -0400 From: Helen E Johnson Subject: [Histonet] marker resistant to triton To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Hi Histonetters, I am inquiring if there is a permanent slide marker that is resistant to the chemical triton when doing IHC. Helen Johnson (hej01@health.state.ny.us) IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. ------------------------------ Message: 21 Date: Thu, 13 Mar 2008 15:00:49 -0000 From: "Weaver, Colin" Subject: [Histonet] Lung processing To: Message-ID: <7A885E8FE1C71C488D974EC601FAA69002E0865A@vla-exchn1.cvlnt.vla.gov.uk> Content-Type: text/plain; charset="us-ascii" Hi - everyone we have just moved from routine processing in a VIP 1000 to a Thermo-Shandon Excelsior. This has been working perfectly except when processing lung, especially spongy lung. When embedding after processing wax infiltration appears to be patchy, bubbles come out of the tissue when pressing the tissue into a mould, after trimming there are "holes" in the tissue, and sections often crumble because of lack of wax support. We have vacuumn on everything from first alcohol onwards. Anyone got any suggestions as to why this is happening? We used to use Thermo-Shandon Hypercenter XP's and had the same problem with lung, is it a Thermo thing? Colin Histology Dept VLA Thirsk Sunny Yorkshire Veterinary Laboratories Agency (VLA) This email and any attachments is intended for the named recipient only. If you have received it in error you have no authority to use, disclose, store or copy any of its contents and you should destroy it and inform the sender. Whilst this email and associated attachments will have been checked for known viruses whilst within VLA systems we can accept no responsibility once it has left our systems. Communications on VLA's computer systems may be monitored and/or recorded to secure the effective operation of the system and for other lawful purposes. ------------------------------ Message: 22 Date: Thu, 13 Mar 2008 08:24:09 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Air bubbles To: Emily Sours , histonet@lists.utsouthwestern.edu Message-ID: <807810.16869.qm@web65703.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Emily: That does not sound stupid, less mounting medium than required cause bubbles formation, as well as too tick mounting medium. Ren? J. Emily Sours wrote: This may sound kind of stupid, but I always thought it might be because more mounting media was needed when coverslipping. A lab down the hall used the same protocol as ours and always had air bubbles after a week. They only used two drops of media for coverslipping, but we use four to five. Emily -- People aren't like chocolates. People are bastards. Bastards with bastard coating and bastard filling. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. ------------------------------ Message: 23 Date: Thu, 13 Mar 2008 08:26:24 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Gills III To: Robyn Vazquez , Histonet@lists.utsouthwestern.edu Message-ID: <605838.80002.qm@web65716.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Opening a hematoxylin 3 months after receiving it cannot be the cause UNLESS it has an expiration date within that period, or is close to expiring (lets say before 2008). Ren? J. Robyn Vazquez wrote: Hello, We have been using Gills III, as of recently approx 1 month, to do frozens on a linear stainer, it has been getting stringy. Not a lot but significantly. We rec'd it in 6/07 and opened it in 9/07. Could it be too old? I tried filtering it, but still stringy. I also, turned up the water to help rinse it, but I don't want to take a chance on losing tissue. Thanks in advanced. Robyn OHSU _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. ------------------------------ Message: 24 Date: Thu, 13 Mar 2008 09:09:48 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Lung processing To: "Weaver, Colin" , histonet@lists.utsouthwestern.edu Message-ID: <380780.53437.qm@web65703.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Colin: You hit "the nail in the head". It is "vacuum thing", I don't know if it is a "Thermo thing". Check their operating manual and find out the vacuum level (mm of Hg) and compare with what the VIP 1000 used to have. If the vacuum is higher in the VIP 1000, that is your answer, if it is the same, then it has to be a "Thermo thing". Ren? J. "Weaver, Colin" wrote: Hi - everyone we have just moved from routine processing in a VIP 1000 to a Thermo-Shandon Excelsior. This has been working perfectly except when processing lung, especially spongy lung. When embedding after processing wax infiltration appears to be patchy, bubbles come out of the tissue when pressing the tissue into a mould, after trimming there are "holes" in the tissue, and sections often crumble because of lack of wax support. We have vacuumn on everything from first alcohol onwards. Anyone got any suggestions as to why this is happening? We used to use Thermo-Shandon Hypercenter XP's and had the same problem with lung, is it a Thermo thing? Colin Histology Dept VLA Thirsk Sunny Yorkshire Veterinary Laboratories Agency (VLA) This email and any attachments is intended for the named recipient only. If you have received it in error you have no authority to use, disclose, store or copy any of its contents and you should destroy it and inform the sender. Whilst this email and associated attachments will have been checked for known viruses whilst within VLA systems we can accept no responsibility once it has left our systems. Communications on VLA's computer systems may be monitored and/or recorded to secure the effective operation of the system and for other lawful purposes. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 52, Issue 19 **************************************** From TJJ <@t> Stowers-Institute.org Thu Mar 13 11:30:56 2008 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Thu Mar 13 11:31:18 2008 Subject: [Histonet] Re: prostate bx falling off slide Message-ID: Steve, The post-fixing the slides in formalin is a good suggestion. Also, try cutting them a bit thinner, 3-4 microns if you're using 5. Additionally, if you need to you might test the antibody on control material (validate!) and see if an 80 degree waterbath for longer (1 hour or so?) keeps them on the slide better than the pressure cooker while still keeping the signal at the desired intensity. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From Jerry <@t> ralambusa.com Thu Mar 13 11:41:52 2008 From: Jerry <@t> ralambusa.com (Jerry Helisek) Date: Thu Mar 13 11:42:01 2008 Subject: [Histonet] Slide Marker Message-ID: <3855F92002259948A66A8CA2D16E3A4F05ACEE@server.ralambusa.com> Helen, this may be overkill, but we manufacture a slide writer for ourselves (RA Lamb), ThermoFisher & TBS. See attached PDF. Thanks, ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, March 13, 2008 12:34 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 52, Issue 19 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. prostate bx falling off slide (crochieresteve@aol.com) 2. HT position available (crochieresteve@aol.com) 3. Microwaves to keep or not to keep (Vacca Jessica) 4. S100P (Kimberly Tuttle) 5. Good Lab Practices (clarification) (Jenee.S.Odani@hawaii.gov) 6. RE: Salary Survey for 2007-2008 (Ellenburg, Deborah) 7. Re: Good Lab Practices (clarification) (Rene J Buesa) 8. Re: prostate bx falling off slide (Rene J Buesa) 9. Doublecortin (Karen Lapanowski) 10. Textbook referrals (Celebre Julia) 11. Re: prostate bx falling off slide (Patricia Valente) 12. Digital Microscope Cameras for Classroom Use (Ross Christian Langston) 13. MMA for Golgi? (Bob Nienhuis) 14. RE: Textbook referrals (Lee & Peggy Wenk) 15. Re: Doublecortin (Olek Michalski) 16. What type media? (Mary Lou Norman) 17. RE: Textbook referrals (Smith, Allen) 18. Re: Air bubbles (Emily Sours) 19. Gills III (Robyn Vazquez) 20. marker resistant to triton (Helen E Johnson) 21. Lung processing (Weaver, Colin) 22. Re: Air bubbles (Rene J Buesa) 23. Re: Gills III (Rene J Buesa) 24. Re: Lung processing (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Wed, 12 Mar 2008 13:08:25 -0400 From: crochieresteve@aol.com Subject: [Histonet] prostate bx falling off slide To: histonet@pathology.swmed.edu Message-ID: <8CA527AD164F369-9A4-11D4@webmail-me06.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Are there any suggestions to help keep prostate core biopsies on the plus slides when doing PIN-4 IHC. I use the Biocare de-cloaker and their BORG retrieval solution and Nemesis autostainer. I have been having a tough time with sections falling away after retrieval. I air dry them overnight and incubate in a 60 degree oven for 1 hour prior to de-wax and staining. What to do?? Steve Crochiere Histology Supervisor Life Path Partners, Mercy Medical Center ------------------------------ Message: 2 Date: Wed, 12 Mar 2008 13:09:54 -0400 From: crochieresteve@aol.com Subject: [Histonet] HT position available To: histonet@pathology.swmed.edu Message-ID: <8CA527B06CE9B3B-9A4-11E6@webmail-me06.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" We are looking for an experienced HT in the Springfield MA area to work in our hospital based laboratory and our satellite prostate lab. Send resume to Steve.crochiere@SPHS.com ------------------------------ Message: 3 Date: Wed, 12 Mar 2008 13:52:27 -0400 From: "Vacca Jessica" Subject: [Histonet] Microwaves to keep or not to keep To: Message-ID: <41E16A15CE78374EA45B57E0F94339B8037DDCC8@ORLEV01.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" I would like to know how many of you are keeping your "household" microwaves? I do a couple of special stains that we heat up the solutions. We don't do any processing with it. Those of you that are keeping them, what are you doing to avoid the ding? Or are you just taking your chances? Which do you recommend for a "lab" microwave? Thanks Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr. Brandon Fl 33511 (813) 571-5193 or (813) 681-5551 ext 2454 Jessica.Vacca@hcahealthcare.com ------------------------------ Message: 4 Date: Wed, 12 Mar 2008 13:57:58 -0400 From: "Kimberly Tuttle" Subject: [Histonet] S100P To: Message-ID: <47D7E165.90CE.001A.3@umm.edu> Content-Type: text/plain; charset=US-ASCII Does anyone know where I can buy S100P, how much it costs, lead time.....Thanks Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. ------------------------------ Message: 5 Date: Wed, 12 Mar 2008 08:10:57 -1000 From: Jenee.S.Odani@hawaii.gov Subject: [Histonet] Good Lab Practices (clarification) To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" I posted yesterday asking if anyone would share their protocols/manuals regarding disposal of blocks/tissues. For clarification, this is not because I need info to write a manual. Our state does not have any clear guidelines, and a USDA official is trying to get an idea of "what other labs do" to help certify a new lab in our state. Can you share with me how your lab disposes of blocks and tissues? What logs or records are kept? Jenee S. Odani, D.V.M., Dipl. ACVP Veterinary Medical Officer Hawaii State Veterinary Laboratory/DAI 99-941 Halawa Valley Street, Aiea, HI, 96701 Phone: (808) 483-7131/Fax: (808) 483-7110 ------------------------------ Message: 6 Date: Wed, 12 Mar 2008 14:27:59 -0400 From: "Ellenburg, Deborah" Subject: [Histonet] RE: Salary Survey for 2007-2008 To: Message-ID: Content-Type: text/plain; charset=iso-8859-1 Advance for Medical Laboratory Professionals is doing an online salary survey and are to have the results of the survey available in June. You can logo to www.advanceweb.com/surveys to participate in the survey. Hope this helps. Debbie Ellenburg, HT (ASCP) Histology Supervisor Bon Secours St. Francis Health System One St. Francis Drive Greenville, SC 29601 864-255-1582 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, March 12, 2008 1:10 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 52, Issue 18 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: salery survey (Rene J Buesa) 2. Book for Fluorescent Staining (Bobrowitz, Carol) 3. Air bubbles (Karen Lapanowski) 4. CSH Symposium (Jennifer MacDonald) 5. RE: Air bubbles (Marshall Terry Dr, Consultant Histopathologist) 6. HMP300 tissue processor (Simon Smith) 7. RE: Book for Fluorescent Staining (Houston, Ronald) ---------------------------------------------------------------------- Message: 1 Date: Wed, 12 Mar 2008 07:11:03 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] salery survey To: richard shook , histonet@lists.utsouthwestern.edu Message-ID: <326455.77544.qm@web65713.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Under separate cover I am sending you my article (Annals of Diagnostic Pathology) on safety. Ren? J. richard shook wrote: A quick question for all is there, or has there been a recent histology salery survey and if so where could i find it. i have seen one before but cant remember where it was thank you rich rabkin derm path ____________________________________________________________________________________ Looking for last minute shopping deals? Find them fast with Yahoo! Search. http://tools.search.yahoo.com/newsearch/category.php?category=shopping _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. ------------------------------ Message: 2 Date: Wed, 12 Mar 2008 10:39:57 -0500 From: "Bobrowitz, Carol" Subject: [Histonet] Book for Fluorescent Staining To: Message-ID: <8F78639AC56F4143B267FE5F5A1B92C8F0AEE0@guyton.phys.mcw.edu> Content-Type: text/plain; charset="us-ascii" Hi, I am looking for a book or information booklet pertaining strictly to fluorescent staining. Something on the order of Dako' s immunohistochemical staining book or C.M. vander Loos's multiple staining book. Even a web page reference would be helpful. I know Galye Callis is giving a workshop this summer in New Mexico, unfortunately I can not attend. Hopefully she will be giving this workshop in Pittsburg. Any information that you can provide me will be greatly appreciated. Thank you in advance for your help. Carol Ann Bobrowitz Department of Physiology Medical College of Wisconsin 414-456-8179 cbobrowi@mcw.edu ------------------------------ Message: 3 Date: Wed, 12 Mar 2008 11:16:58 -0400 From: "Karen Lapanowski" Subject: [Histonet] Air bubbles To: histonet@lists.utsouthwestern.edu Message-ID: <20080312T111658Z_02DE000F0000@hfhs.org> Content-Type: text/plain; charset=utf-8 Hello, Air bubbles develop a week or so after coverslipping sections using Vectashield hardset mounting media. (Initially, no air pockets were visible.) Any suggestions? Thanks, Karen ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== ------------------------------ Message: 4 Date: Wed, 12 Mar 2008 09:26:30 -0700 From: Jennifer MacDonald Subject: [Histonet] CSH Symposium To: histonet@lists.utsouthwestern.edu Cc: lfigueroa@sakuraus.com Message-ID: Content-Type: text/plain; charset="US-ASCII" The California Society for Histotechnology annual symposium will be held at the Ventura Beach Marriott in Ventura, California. Please visit our website for the complete program. Thank you, http://www.californiahistology.org/ Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu ------------------------------ Message: 5 Date: Wed, 12 Mar 2008 16:31:39 -0000 From: "Marshall Terry Dr, Consultant Histopathologist" Subject: RE: [Histonet] Air bubbles To: "Karen Lapanowski" , Message-ID: <5C0BED61F529364E86309CADEA63FEF20163F387@TRFT-EX01.xRothGen.nhs.uk> Content-Type: text/plain; charset="us-ascii" We are getting the same problem, except that it is (as yours probably is) "dryback". Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Karen Lapanowski Sent: 12 March 2008 15:17 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Air bubbles Hello, Air bubbles develop a week or so after coverslipping sections using Vectashield hardset mounting media. (Initially, no air pockets were visible.) Any suggestions? Thanks, Karen ======================================================================== ====== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ======================================================================== ====== ###################################################################### This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. ###################################################################### ------------------------------ Message: 6 Date: Wed, 12 Mar 2008 09:32:07 -0700 From: "Simon Smith" Subject: [Histonet] HMP300 tissue processor To: Message-ID: Content-Type: text/plain; charset="us-ascii" Folks, I have an old but good Microm/Hacker HMP300 tissue processor which is refusing to behave at the moment. When trying to run it fails at diagnostic #3, A more detailed check reveals the problem to be a "Station valve driver fail off" error for station 14 (the xylene cleaning step). I have obtained and replaced the valve to no effect, I swapped the electrical connections between station 14 and 15 (I still get the same error for station 14) and am at the end of my patience with the wee beastie. I have the following questions: 1. What is causing the problem? 2. How do I fix it? 3. Is there anyone out there who remembers these machines and can fix the problem? I am located just North of Seattle. 4. Failing that, does anyone have a service manual and/or parts I can borrow or buy? Thanks in advance for your help Simon ------------------------------ Message: 7 Date: Wed, 12 Mar 2008 12:49:40 -0400 From: "Houston, Ronald" Subject: RE: [Histonet] Book for Fluorescent Staining To: "Bobrowitz, Carol" , Message-ID: <979FF5962E234F45B06CF0DB7C1AABB215E8CF1A@chi2k3ms01.columbuschildrens.net> Content-Type: text/plain; charset="us-ascii" Protein Localization by Fluorescence Microscopy: a practical approach Ed. VJ Allen Pub OUP, 2000 ISBN: 0-19-963741-5 (hbk) 0-19-963740-7 (pbk) Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 Ronald.Houston@NationwideChildrens.org Columbus Children's Hospital is now Nationwide Children's Hospital www.NationwideChildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bobrowitz, Carol Sent: Wednesday, March 12, 2008 11:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Book for Fluorescent Staining Hi, I am looking for a book or information booklet pertaining strictly to fluorescent staining. Something on the order of Dako' s immunohistochemical staining book or C.M. vander Loos's multiple staining book. Even a web page reference would be helpful. I know Galye Callis is giving a workshop this summer in New Mexico, unfortunately I can not attend. Hopefully she will be giving this workshop in Pittsburg. Any information that you can provide me will be greatly appreciated. Thank you in advance for your help. Carol Ann Bobrowitz Department of Physiology Medical College of Wisconsin 414-456-8179 cbobrowi@mcw.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 52, Issue 18 **************************************** ________________________________________________________________________________________________________________________________ ________________________________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ------------------------------ Message: 7 Date: Wed, 12 Mar 2008 12:38:11 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Good Lab Practices (clarification) To: Jenee.S.Odani@hawaii.gov, histonet@lists.utsouthwestern.edu Message-ID: <200124.30165.qm@web65713.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 We used to incinerate them after keeping them for the prescribed time period. Ren? J. Jenee.S.Odani@hawaii.gov wrote: I posted yesterday asking if anyone would share their protocols/manuals regarding disposal of blocks/tissues. For clarification, this is not because I need info to write a manual. Our state does not have any clear guidelines, and a USDA official is trying to get an idea of "what other labs do" to help certify a new lab in our state. Can you share with me how your lab disposes of blocks and tissues? What logs or records are kept? Jenee S. Odani, D.V.M., Dipl. ACVP Veterinary Medical Officer Hawaii State Veterinary Laboratory/DAI 99-941 Halawa Valley Street, Aiea, HI, 96701 Phone: (808) 483-7131/Fax: (808) 483-7110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. ------------------------------ Message: 8 Date: Wed, 12 Mar 2008 12:42:54 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] prostate bx falling off slide To: crochieresteve@aol.com, histonet@pathology.swmed.edu Message-ID: <307439.49289.qm@web65706.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Check your processing protocol. Maybe they are treated too long in dehydrant/clearant. How do they section, brittle? If that is the case it is an indicaiton of a too prolonged processing protocol. Ren? J. crochieresteve@aol.com wrote: Are there any suggestions to help keep prostate core biopsies on the plus slides when doing PIN-4 IHC. I use the Biocare de-cloaker and their BORG retrieval solution and Nemesis autostainer. I have been having a tough time with sections falling away after retrieval. I air dry them overnight and incubate in a 60 degree oven for 1 hour prior to de-wax and staining. What to do?? Steve Crochiere Histology Supervisor Life Path Partners, Mercy Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. ------------------------------ Message: 9 Date: Wed, 12 Mar 2008 16:24:40 -0400 From: "Karen Lapanowski" Subject: [Histonet] Doublecortin To: histonet@lists.utsouthwestern.edu Message-ID: <20080312T162440Z_02DE000F0000@hfhs.org> Content-Type: text/plain; charset=utf-8 Hello, What supplier do you use for doublecortin antibody? Will be staining paraffin sections using FITC as the secondary. Thanks, Karen Lapanowski Karen Lapanowski Radiation Oncology 3065 E & R 313-916-9386 ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== ------------------------------ Message: 10 Date: Wed, 12 Mar 2008 16:41:13 -0400 From: "Celebre Julia" Subject: [Histonet] Textbook referrals To: Message-ID: <4D3667D4B5487546A3139FB918181FEA078E34@ipemail01.hhsc.ca> Content-Type: text/plain; charset="iso-8859-1" Believe it or not, but there are funds available to us for purchasing new Histology textbooks. I'm wondering if anyone in Histoland could kindly suggest some good textbooks that are now available. Julia Celebre MLT Anatomic Pathology Hamilton General Hospital 905-527-0271 ext 46179 email: celebrej@hhsc.ca This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. ------------------------------ Message: 11 Date: Wed, 12 Mar 2008 13:47:41 -0700 (PDT) From: Patricia Valente Subject: Re: [Histonet] prostate bx falling off slide To: Histonet@lists.utsouthwestern.edu Message-ID: <447310.77002.qm@web81703.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 After dewaxing the slides and taking down to water try 10 minutes in 10%NBF, rinse well before retrieval. Pat valente uropathllc --- crochieresteve@aol.com wrote: > Are there any suggestions to help keep prostate core > biopsies on the plus slides when doing PIN-4 IHC. I > use the Biocare de-cloaker and their BORG retrieval > solution and Nemesis autostainer. I have been having > a tough time with sections falling away after > retrieval. I air dry them overnight and incubate in > a 60 degree oven for 1 hour prior to de-wax and > staining. What to do?? > Steve Crochiere > Histology Supervisor > Life Path Partners, Mercy Medical Center > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Pat ------------------------------ Message: 12 Date: Wed, 12 Mar 2008 10:55:53 -1000 From: Ross Christian Langston Subject: [Histonet] Digital Microscope Cameras for Classroom Use To: Karen Lapanowski Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Hi Folks- could anyone with experience with either of the following two microscope cameras weigh-in on their merits or drawbacks? We are planning on projecting the feed onto a screen in our anatomy and physiology and lab techniques classes. Thanks in advance for your input! Ross The cameras: Q imaging Micropublisher 5.0 (uncooled) Lumenera Infinity 1-1 Ross Langston, PhD Department of Natural Sciences Windward Community College 45-720 Keaahala Road Kaneohe, Hawaii 96744 808-236-9119 (office) 808-247-5362 (fax) ----- Original Message ----- From: Karen Lapanowski Date: Wednesday, March 12, 2008 10:26 am Subject: [Histonet] Doublecortin To: histonet@lists.utsouthwestern.edu > Hello, > What supplier do you use for doublecortin antibody? Will be > staining paraffin sections using FITC as the secondary. > Thanks, > Karen Lapanowski > > Karen Lapanowski > Radiation Oncology > 3065 E & R > 313-916-9386 > ============================================================================== > CONFIDENTIALITY NOTICE: This email contains information from the > sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY > or otherwise protected from disclosure. This email is intended for > use only by the person or entity to whom it is addressed. If you > are not the intended recipient, any use, disclosure, copying, > distribution, printing, or any action taken in reliance on the > contents of this email, is strictly prohibited. If you received > this email in error, please contact the sending party by reply > email, delete the email from your computer system and shred any > paper copies. > > Note to Patients: There are a number of risks you should consider > before using e-mail to communicate with us. See our Privacy Policy > and Henry Ford My Health at www.henryford.com for more detailed > information. If you do not believe that our policy gives you the > privacy and security protection you need, do not send e-mail or > Internet communications to us. > > ============================================================================== > ------------------------------ Message: 13 Date: Wed, 12 Mar 2008 14:47:47 -0800 From: "Bob Nienhuis" Subject: [Histonet] MMA for Golgi? To: Histonet Message-ID: <45109da50803121547w1c69afb5k64fb11c61c61381b@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Someone suggested the possibility of using MMA as an embedding medium instead of celloidin for rapid Golgi. How is it made soft enough for cutting with a microtome? Anyone have experience with this, or have a protocol for doing this? Any thoughts or comments? I have looked at the Neil Hand papers, but they don't seen to exactly apply to what I want to do. I am looking to do thick sections so as to see full dendritic arborization. Bob Nienhuis UCLA / VA Medical Center Neurobiology Research North Hills, CA 91343 ------------------------------ Message: 14 Date: Thu, 13 Mar 2008 05:37:53 -0400 From: "Lee & Peggy Wenk" Subject: RE: [Histonet] Textbook referrals To: "'Celebre Julia'" , Message-ID: <000301c884ed$f1ab4c70$0202a8c0@HPPav2> Content-Type: text/plain; charset="us-ascii" NSH website has a list of books that are available and current, and were suggested by Histonet members. www.nsh.org Highlight Career Center on left Click on Tools and Resources Click on Reference Materials Books listed by topic: - Histotechnology - Management/Education - Immunohistochemistry - Molecular Pathology - Histology Atlas - Lab Math - Lab Safety - Misc. (skin, cytoprep) Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Celebre Julia Sent: Wednesday, March 12, 2008 4:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Textbook referrals Believe it or not, but there are funds available to us for purchasing new Histology textbooks. I'm wondering if anyone in Histoland could kindly suggest some good textbooks that are now available. Julia Celebre MLT Anatomic Pathology Hamilton General Hospital 905-527-0271 ext 46179 email: celebrej@hhsc.ca This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Thu, 13 Mar 2008 13:34:10 +0100 From: "Olek Michalski" Subject: Re: [Histonet] Doublecortin To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format=flowed; delsp=yes; charset=iso-8859-2 I use Santa Cruz Biochemicals and the antibody (produced in goat) works perfectly. Best Regards Olek Michalski 2008-03-12 21:24:40 Karen Lapanowski wrote: > Hello, > What supplier do you use for doublecortin antibody? Will be staining > paraffin sections using FITC as the secondary. > Thanks, > Karen Lapanowski > > Karen Lapanowski > Radiation Oncology > 3065 E & R > 313-916-9386 > ============================================================================== > CONFIDENTIALITY NOTICE: This email contains information from the sender > that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise > protected from disclosure. This email is intended for use only by the > person or entity to whom it is addressed. If you are not the intended > recipient, any use, disclosure, copying, distribution, printing, or any > action taken in reliance on the contents of this email, is strictly > prohibited. If you received this email in error, please contact the > sending party by reply email, delete the email from your computer system > and shred any paper copies. > Note to Patients: There are a number of risks you should consider before > using e-mail to communicate with us. See our Privacy Policy and Henry > Ford My Health at www.henryford.com for more detailed information. If > you do not believe that our policy gives you the privacy and security > protection you need, do not send e-mail or Internet communications to us. > > ============================================================================== -- Laboratory of Neurobiology of Development and Evolution Nencki Institute of Experimental Biology ul. Pasteura 3, 02-093 Warszawa, Poland Tel. +48 22 5892268, Fax +48 22 8225342 ------------------------------ Message: 16 Date: Thu, 13 Mar 2008 09:09:00 -0400 From: Mary Lou Norman Subject: [Histonet] What type media? To: histonet@lists.utsouthwestern.edu Message-ID: <6.2.1.2.2.20080313085627.03c0a720@postoffice9.mail.cornell.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Hello, I don't do immuno so I am clueless. Please tell me the best media to grow lung cells on so I can prepare them later for paraffin histopath. H&E. Thank you, Mary Lou ------------------------------ Message: 17 Date: Thu, 13 Mar 2008 09:07:27 -0400 From: "Smith, Allen" Subject: [Histonet] RE: Textbook referrals To: 'Celebre Julia' Cc: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" HISTOLOGY: A TEXT AND ATLAS, 5th ed. by Michael Ross and Wojciech Pawlina (long and detailed) WHEATER'S FUNCTIONAL HISTOLOGY, 4th ed. by Barbara Young (very concise) Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Celebre Julia Sent: Wednesday, March 12, 2008 3:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Textbook referrals Believe it or not, but there are funds available to us for purchasing new Histology textbooks. I'm wondering if anyone in Histoland could kindly suggest some good textbooks that are now available. Julia Celebre MLT Anatomic Pathology Hamilton General Hospital 905-527-0271 ext 46179 email: celebrej@hhsc.ca This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Thu, 13 Mar 2008 09:51:39 -0400 From: "Emily Sours" Subject: Re: [Histonet] Air bubbles To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 This may sound kind of stupid, but I always thought it might be because more mounting media was needed when coverslipping. A lab down the hall used the same protocol as ours and always had air bubbles after a week. They only used two drops of media for coverslipping, but we use four to five. Emily -- People aren't like chocolates. People are bastards. Bastards with bastard coating and bastard filling. ------------------------------ Message: 19 Date: Thu, 13 Mar 2008 07:04:02 -0700 From: "Robyn Vazquez" Subject: [Histonet] Gills III To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Hello, We have been using Gills III, as of recently approx 1 month, to do frozens on a linear stainer, it has been getting stringy. Not a lot but significantly. We rec'd it in 6/07 and opened it in 9/07. Could it be too old? I tried filtering it, but still stringy. I also, turned up the water to help rinse it, but I don't want to take a chance on losing tissue. Thanks in advanced. Robyn OHSU ------------------------------ Message: 20 Date: Thu, 13 Mar 2008 10:09:32 -0400 From: Helen E Johnson Subject: [Histonet] marker resistant to triton To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Hi Histonetters, I am inquiring if there is a permanent slide marker that is resistant to the chemical triton when doing IHC. Helen Johnson (hej01@health.state.ny.us) IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. ------------------------------ Message: 21 Date: Thu, 13 Mar 2008 15:00:49 -0000 From: "Weaver, Colin" Subject: [Histonet] Lung processing To: Message-ID: <7A885E8FE1C71C488D974EC601FAA69002E0865A@vla-exchn1.cvlnt.vla.gov.uk> Content-Type: text/plain; charset="us-ascii" Hi - everyone we have just moved from routine processing in a VIP 1000 to a Thermo-Shandon Excelsior. This has been working perfectly except when processing lung, especially spongy lung. When embedding after processing wax infiltration appears to be patchy, bubbles come out of the tissue when pressing the tissue into a mould, after trimming there are "holes" in the tissue, and sections often crumble because of lack of wax support. We have vacuumn on everything from first alcohol onwards. Anyone got any suggestions as to why this is happening? We used to use Thermo-Shandon Hypercenter XP's and had the same problem with lung, is it a Thermo thing? Colin Histology Dept VLA Thirsk Sunny Yorkshire Veterinary Laboratories Agency (VLA) This email and any attachments is intended for the named recipient only. If you have received it in error you have no authority to use, disclose, store or copy any of its contents and you should destroy it and inform the sender. Whilst this email and associated attachments will have been checked for known viruses whilst within VLA systems we can accept no responsibility once it has left our systems. Communications on VLA's computer systems may be monitored and/or recorded to secure the effective operation of the system and for other lawful purposes. ------------------------------ Message: 22 Date: Thu, 13 Mar 2008 08:24:09 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Air bubbles To: Emily Sours , histonet@lists.utsouthwestern.edu Message-ID: <807810.16869.qm@web65703.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Emily: That does not sound stupid, less mounting medium than required cause bubbles formation, as well as too tick mounting medium. Ren? J. Emily Sours wrote: This may sound kind of stupid, but I always thought it might be because more mounting media was needed when coverslipping. A lab down the hall used the same protocol as ours and always had air bubbles after a week. They only used two drops of media for coverslipping, but we use four to five. Emily -- People aren't like chocolates. People are bastards. Bastards with bastard coating and bastard filling. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. ------------------------------ Message: 23 Date: Thu, 13 Mar 2008 08:26:24 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Gills III To: Robyn Vazquez , Histonet@lists.utsouthwestern.edu Message-ID: <605838.80002.qm@web65716.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Opening a hematoxylin 3 months after receiving it cannot be the cause UNLESS it has an expiration date within that period, or is close to expiring (lets say before 2008). Ren? J. Robyn Vazquez wrote: Hello, We have been using Gills III, as of recently approx 1 month, to do frozens on a linear stainer, it has been getting stringy. Not a lot but significantly. We rec'd it in 6/07 and opened it in 9/07. Could it be too old? I tried filtering it, but still stringy. I also, turned up the water to help rinse it, but I don't want to take a chance on losing tissue. Thanks in advanced. Robyn OHSU _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. ------------------------------ Message: 24 Date: Thu, 13 Mar 2008 09:09:48 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Lung processing To: "Weaver, Colin" , histonet@lists.utsouthwestern.edu Message-ID: <380780.53437.qm@web65703.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Colin: You hit "the nail in the head". It is "vacuum thing", I don't know if it is a "Thermo thing". Check their operating manual and find out the vacuum level (mm of Hg) and compare with what the VIP 1000 used to have. If the vacuum is higher in the VIP 1000, that is your answer, if it is the same, then it has to be a "Thermo thing". Ren? J. "Weaver, Colin" wrote: Hi - everyone we have just moved from routine processing in a VIP 1000 to a Thermo-Shandon Excelsior. This has been working perfectly except when processing lung, especially spongy lung. When embedding after processing wax infiltration appears to be patchy, bubbles come out of the tissue when pressing the tissue into a mould, after trimming there are "holes" in the tissue, and sections often crumble because of lack of wax support. We have vacuumn on everything from first alcohol onwards. Anyone got any suggestions as to why this is happening? We used to use Thermo-Shandon Hypercenter XP's and had the same problem with lung, is it a Thermo thing? Colin Histology Dept VLA Thirsk Sunny Yorkshire Veterinary Laboratories Agency (VLA) This email and any attachments is intended for the named recipient only. If you have received it in error you have no authority to use, disclose, store or copy any of its contents and you should destroy it and inform the sender. Whilst this email and associated attachments will have been checked for known viruses whilst within VLA systems we can accept no responsibility once it has left our systems. Communications on VLA's computer systems may be monitored and/or recorded to secure the effective operation of the system and for other lawful purposes. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 52, Issue 19 **************************************** From MMargiotta <@t> bmhmc.org Thu Mar 13 11:42:48 2008 From: MMargiotta <@t> bmhmc.org (Margiotta, Michele) Date: Thu Mar 13 11:42:53 2008 Subject: [Histonet] silver waste Message-ID: Hi All, We use a silver recovery system for our waste silver from our special stains. We found out that the company that took care of that for us went out of business. Are most labs just discarding it as hazardous waste? Does anyone have any info about who we can contact to continue using this system. If so, please let me know. Thanks!! Michele Margiotta BMHMC Histology Supervisor 631-654-7192 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. From cormier <@t> MIT.EDU Thu Mar 13 11:57:00 2008 From: cormier <@t> MIT.EDU (Kathy Cormier) Date: Thu Mar 13 11:56:57 2008 Subject: [Histonet] amsterdam solution? Message-ID: <001001c8852b$41f51d90$92003712@mit.edu> Hey Netters, Does anyone know what Amsterdam solution is and what the componets are? I have a researcher who just dropped off a paper mentioning that tissues were fixed in "Amsterdam solution".... Thanks for the help... Kathy DCM MIT From alonso.martinezcanabal <@t> utoronto.ca Thu Mar 13 12:13:14 2008 From: alonso.martinezcanabal <@t> utoronto.ca (alonso.martinezcanabal@utoronto.ca) Date: Thu Mar 13 12:13:58 2008 Subject: [Histonet] About marking brain hemisphers Message-ID: <20080313131314.uoad9i9kak0ss4g8@webmail.utoronto.ca> Hi everyone, I have a question for you, I am slicing a bunch of mice brains in vibratome and I want to mount after the sections, the analysis that I want to do is sensitive to the anatomy, so, Is important for me to keep a good track of wich hemisphere corresponds with which, so basically I want to know if there some way to mark the ?sides of the brain? that does not affect further immunohistochemistry or Nissl staining. Thank you very much. See you Alonso From jcline <@t> wchsys.org Thu Mar 13 12:14:54 2008 From: jcline <@t> wchsys.org (Joyce Cline) Date: Thu Mar 13 12:15:06 2008 Subject: [Histonet] Good Lab Practices (clarification) In-Reply-To: Message-ID: <002001c8852d$c34d3a60$1d2a14ac@wchsys.org> We have our blocks and slides hauled by the same company that picks up our alcohol/substitute waste. The blocks are packed in cardboard drums and incinerated. I am in Maryland you can go to the site for regulated medical waste resource locator on line. www.envcap.org/statetools/rmw/md-rmw.cfm I found this site useful -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jenee.S.Odani@hawaii.gov Sent: Wednesday, March 12, 2008 2:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Good Lab Practices (clarification) I posted yesterday asking if anyone would share their protocols/manuals regarding disposal of blocks/tissues. For clarification, this is not because I need info to write a manual. Our state does not have any clear guidelines, and a USDA official is trying to get an idea of "what other labs do" to help certify a new lab in our state. Can you share with me how your lab disposes of blocks and tissues? What logs or records are kept? Jenee S. Odani, D.V.M., Dipl. ACVP Veterinary Medical Officer Hawaii State Veterinary Laboratory/DAI 99-941 Halawa Valley Street, Aiea, HI, 96701 Phone: (808) 483-7131/Fax: (808) 483-7110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From trathborne <@t> somerset-healthcare.com Thu Mar 13 12:19:29 2008 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Mar 13 12:20:03 2008 Subject: [Histonet] microtome calibration In-Reply-To: <0E6BC087F70F9C47ACFF2C203D6E329C03CBFE33@NTRSEVS30002.s3.ms.unilever.com> Message-ID: You can try Belair Instruments in Springfield, NJ. Their # is 800-783-9424 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Kalleberg, Kristopher Sent: Thursday, March 13, 2008 12:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] microtome calibration Does anyone know of anyone company that calibrates microtomes? I currently have an old Leitz 1512 microtome and I was just recently informed by leica that they no longer service or calibrate these microtomes. If anyone knows of a company that calibrates this microtome I would appreciate any info. The company would have to be in the Connecticut/ tri-State area. Thanks. Kris Kalleberg Research Scientist Unilever R&D 40 Merritt Blvd. Trumbull, CT 06611 (203) 381-5765 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. From Susan.Pemberton <@t> bmcjax.com Thu Mar 13 12:49:40 2008 From: Susan.Pemberton <@t> bmcjax.com (Pemberton, Susan) Date: Thu Mar 13 12:50:09 2008 Subject: [Histonet] Histology Supervisor Position Message-ID: <4E59E1E7B5A80F448F47424C8A2FDEDB01493DE3@BHEXCHVS02.BH.LOCAL> Baptist Medical Center, Jacksonville, FL, is seeking a fulltime Histology Supervisor to help with the daily operations of a large hospital based histology laboratory. Baptist Health is a four-hospital not-for-profit health system. Baptist Medical Center-Downtown houses the core laboratory for the system and is located on the beautiful St. John's River in downtown Jacksonville, 12 miles from the Atlantic Ocean. The laboratory processes approximately 400 blocks per day and performs extensive immunohistochemistry. The supervisor position reports to the Manager of Anatomic Pathology. Previous management experience, experience with regulatory requirements and certification as HTL(ASCP) is preferred. The position requires a Florida license which can be obtained by reciprocity with the ASCP certification. If interested, apply at http://community.e-baptisthealth.com/careers/index.html Susan L. Pemberton MS, MT(ASCP)SM,DLM Laboratory Administrative Director . Baptist Health 800 Prudential Drive . Jacksonville, FL 32207 (904) 202-2016 . Fax: (904) 202-2795 ----------------------------------------- NOTICE: This message is confidential, intended for the named recipient(s) and may contain information that is (i) proprietary to the sender, and/or,(ii) privileged, confidential and/or otherwise exempt from disclosure under applicable Florida and federal law, including, but not limited to, privacy standards imposed pursuant to the federal Health insurance Portability and Accountability Act of 1996 ("HIPAA"). Receipt by anyone other than the named recipient(s) is not a waiver of any applicable privilege. Thank you in advance for your compliance with this notice. From leon.brokken <@t> med.lu.se Thu Mar 13 13:10:34 2008 From: leon.brokken <@t> med.lu.se (Leon Brokken) Date: Thu Mar 13 13:10:47 2008 Subject: [Histonet] amsterdam solution? In-Reply-To: <001001c8852b$41f51d90$92003712@mit.edu> References: <001001c8852b$41f51d90$92003712@mit.edu> Message-ID: <47D96E1A.3010802@med.lu.se> Kathy Cormier wrote: > Hey Netters, > > Does anyone know what Amsterdam solution is http://tinyurl.com/ly4f9 > and what the componets are? Tetrahydrocannabinol Sorry, couldn't let that one pass ;-) Amsterdam Fixative: a mixture of methanol/acetone/acetic acid/water (35:35:5:25 (v/v) Cheers, Leon. > I have a researcher who just dropped off a paper mentioning that tissues were fixed in "Amsterdam solution".... > > Thanks for the help... > > Kathy > DCM MIT > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From MThiel <@t> lexpharma.com Thu Mar 13 13:13:35 2008 From: MThiel <@t> lexpharma.com (Thiel, Mary) Date: Thu Mar 13 13:13:38 2008 Subject: [Histonet] Books ? Message-ID: <7503733FE451D3479B6E8BBB554B1C5B0182A61A@wdexchmb01.lexicon.lexgen.com> Does anyone out there know of a good place to get Sheehan's Theory and Practice of Histotechnology ?? Oh, yes for less than $200.00. Thanks, Mary Mary Thiel HT (ASCP) Sr. Research Associate Lexicon Pharmaceuticals 8800 Technology Forest Place The Woodlands, Tx. 77381 Phone: 281-863-3347 email: mthiel@lexpharma.com The contents of this communication, including any attachments, may be confidential, privileged or otherwise protected from disclosure. They are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please do not read, copy, use or disclose the contents of this communication. Please notify the sender immediately and delete the communication in its entirety. From Dorothy.L.Webb <@t> HealthPartners.Com Thu Mar 13 13:21:18 2008 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Thu Mar 13 13:21:26 2008 Subject: [Histonet] Reagent dating Message-ID: <0E394B648E5284478A6CCB78E5AFDA2705635540@hpes1.HealthPartners.int> I am interested to know if everyone is still marking the "Date received" and/or "Date opened" on each reagent and if this is necessary according to regulations? I had heard at a workshop that if the expiration date is on the container, this was not a necessity!! I am rewriting some procedures and was hoping I could change this!! Thanks ahead of time for any helpful explanations!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From nancy_schmitt <@t> pa-ucl.com Thu Mar 13 13:54:53 2008 From: nancy_schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Thu Mar 13 13:55:06 2008 Subject: [Histonet] mounting medium Message-ID: <9FC023A4AB52BB4D87DC6456081A822C087C1B@mercury.pa-ucl.com> Hi to all Looking for information about the difference between High Viscosity (280) and Low Viscosity (60) Mounting mediums. High viscosity is used for Cyto slides as it is thicker and easier to use with the cyto smears that are often times thicker. We also have some cyto slides that are coverslipped with the lower viscosity medium. Does anyone know of any long term affects? Any cloudiness issues with the thicker medium? Is there any reason why we can't use the low viscosity medium (60) for all slides? Thanks for your help Nancy Schmitt Histology Coordinator Dubuque, Iowa NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From vazquezr <@t> ohsu.edu Thu Mar 13 13:36:48 2008 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Thu Mar 13 14:14:41 2008 Subject: [Histonet] Gills III Message-ID: I will check and thanks to all for some ideas. Robyn >>> "Rene J Buesa" 3/13/2008 8:26 AM >>> Opening a hematoxylin 3 months after receiving it cannot be the cause UNLESS it has an expiration date within that period, or is close to expiring (lets say before 2008). Ren? J. Robyn Vazquez wrote: Hello, We have been using Gills III, as of recently approx 1 month, to do frozens on a linear stainer, it has been getting stringy. Not a lot but significantly. We rec'd it in 6/07 and opened it in 9/07. Could it be too old? I tried filtering it, but still stringy. I also, turned up the water to help rinse it, but I don't want to take a chance on losing tissue. Thanks in advanced. Robyn OHSU _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From ASelf <@t> georgetownhospitalsystem.org Thu Mar 13 14:42:45 2008 From: ASelf <@t> georgetownhospitalsystem.org (Amy Self) Date: Thu Mar 13 14:42:55 2008 Subject: [Histonet] Tracking Specimens Message-ID: Dear Histonetters, How are Histology Labs keeping an audit trail to track specimens from the time that they are collected, are picked up from the physicians' offices by couriers, and then delivered to the Lab that performs the histology testing? What kinds of logs are being kept? Is anyone using a barcode system? Do you have an Outreach program? We need feedback on other labs processes? Thanks in advance for your help. Amy Self Georgetown Hospital System NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From rjbuesa <@t> yahoo.com Thu Mar 13 15:22:54 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 13 15:22:58 2008 Subject: [Histonet] Reagent dating In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA2705635540@hpes1.HealthPartners.int> Message-ID: <925164.10604.qm@web65706.mail.ac4.yahoo.com> That is an internal control that I like, if only to develop in my staff the discipline to pay attention to detail. Ren? J. "Webb, Dorothy L" wrote: I am interested to know if everyone is still marking the "Date received" and/or "Date opened" on each reagent and if this is necessary according to regulations? I had heard at a workshop that if the expiration date is on the container, this was not a necessity!! I am rewriting some procedures and was hoping I could change this!! Thanks ahead of time for any helpful explanations!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From rjbuesa <@t> yahoo.com Thu Mar 13 15:33:11 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 13 15:33:14 2008 Subject: [Histonet] mounting medium In-Reply-To: <9FC023A4AB52BB4D87DC6456081A822C087C1B@mercury.pa-ucl.com> Message-ID: <292477.13530.qm@web65705.mail.ac4.yahoo.com> None whatsoever. They are designed to be used as you describe and no ill effects should be expected if used properly. Ren? J. Nancy Schmitt wrote: --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From liz <@t> premierlab.com Thu Mar 13 15:37:48 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Mar 13 15:37:52 2008 Subject: [Histonet] antibody to mycoplasma bovis Message-ID: Hello All Is there anyone out there that is aware of a commercial source for an antibody to mycoplasma bovis for paraffin sections. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 From conniegrubaugh <@t> hotmail.com Thu Mar 13 19:01:46 2008 From: conniegrubaugh <@t> hotmail.com (connie grubaugh) Date: Thu Mar 13 19:01:51 2008 Subject: [Histonet] Nevada Society of Histotechnology Message-ID: I mad a mistake the date of our seminar is May 3rd Saturday not May 2nd as I previously stated. Connie G. _________________________________________________________________ Shed those extra pounds with MSN and The Biggest Loser! http://biggestloser.msn.com/ From immrstambo <@t> hotmail.com Thu Mar 13 20:17:59 2008 From: immrstambo <@t> hotmail.com (Christine Tambasco) Date: Thu Mar 13 20:18:04 2008 Subject: FW: [Histonet] Reagent dating In-Reply-To: <925164.10604.qm@web65706.mail.ac4.yahoo.com> References: <0E394B648E5284478A6CCB78E5AFDA2705635540@hpes1.HealthPartners.int> <925164.10604.qm@web65706.mail.ac4.yahoo.com> Message-ID: Last time I had an inspection from JACHO they suggested we not only date the received date, but also the open date....makes since if you a rotating inventory. Christine Tambasco, HT(ASCP)St. Mary's Hospital Amsterdam, NY> Date: Thu, 13 Mar 2008 13:22:54 -0700> From: rjbuesa@yahoo.com> To: Dorothy.L.Webb@HealthPartners.Com; histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Reagent dating> CC: > > That is an internal control that I like, if only to develop in my staff the discipline to pay attention to detail.> Ren? J.> > "Webb, Dorothy L" wrote:> I am interested to know if everyone is still marking the "Date received"> and/or "Date opened" on each reagent and if this is necessary according> to regulations? I had heard at a workshop that if the expiration date> is on the container, this was not a necessity!! I am rewriting some> procedures and was hoping I could change this!! Thanks ahead of time> for any helpful explanations!!> > Dorothy Webb, HT (ASCP)> Histology Technical Supervisor > Regions Hospital, Pathology Department > 640 Jackson Street, Saint Paul, MN 55101-2595 > Phone: 651-254-2962> Fax: 651-254-2741 > Regions Hospital is part of the HealthPartners family of care> ________________________________________> This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited.> > If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us.> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > ---------------------------------> Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now.> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Helping your favorite cause is as easy as instant messaging.?You IM, we give. http://im.live.com/Messenger/IM/Home/?source=text_hotmail_join From jnocito <@t> satx.rr.com Thu Mar 13 20:45:29 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Mar 13 20:45:16 2008 Subject: [Histonet] Reagent dating References: <0E394B648E5284478A6CCB78E5AFDA2705635540@hpes1.HealthPartners.int> Message-ID: <00c301c88575$15c2b990$0302a8c0@yourxhtr8hvc4p> Dorothy, dating reagents is only required when opened and/or made up. Date received is no longer required, but we still use it so we know when to rotate our stock. JTT ----- Original Message ----- From: "Webb, Dorothy L" To: Sent: Thursday, March 13, 2008 1:21 PM Subject: [Histonet] Reagent dating I am interested to know if everyone is still marking the "Date received" and/or "Date opened" on each reagent and if this is necessary according to regulations? I had heard at a workshop that if the expiration date is on the container, this was not a necessity!! I am rewriting some procedures and was hoping I could change this!! Thanks ahead of time for any helpful explanations!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kdwyer3322 <@t> aol.com Thu Mar 13 20:48:44 2008 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Thu Mar 13 20:48:51 2008 Subject: [Histonet] Texas Society for Histotechnology Symposium/Convention April 18-20, 2008 Message-ID: <8CA538CABCF5B0A-1594-1ED6@FWM-M15.sysops.aol.com> Hi Histonetters! The Texas Society for Histotechnology will be hosting the annual Symposium/Convention, April 18-20, 2008 at the DFW Hilton Lakes, 1800 Hwy 26, Grapevine,Texas.? Golf Tournanment will be hosted on April 17, 2008 at 1:00pm to register for golf contact mhale@carisdx.com .? If you would like a copy of the program or more information please reply to this e-mail. Workshops include: WS 1:? Small Specimen Management (In a Large Volume World): Skip Brown, M.Div., HT (ASCP) WS 2: ?A Review of the Human Cell and an Introduction to Cell Injury: Mark Bailey, MA, HT (ASCP) WS 3: Are You Prepared to Take the HT (ASCP) Registry Exam: Robert Lott, BS., HTL(ASCP) WS 4: Cytology for Histotechnologist: From Tissue to Liquids and Everything in Between: T.W. Crook, M.D. and Thuy Ardaman, M.D. WS 5: Laboratory Safety-What You Need to Know: Jason Burill, Histology Manager WS 6: HQUIP: Freida L. Carson, PhD; Sue E. Lewis, BS, HTL(ASCP) WS 7: Principles of Grossing: Steve Eagle, RS, PA/ASCP eligible? WS 8: Introduction to Immunos: Bonnie Whitaker, HT (ASCP)QIHC WS 9: Is Your Tissue Processor Fighting You? Fight Back: Pam Marcum HT(ASCP) WS 10: LEADERSHIP JAZZ: Developing Alignment & Synchronicity in Lab Operations: Skip Brown, M. Div., HT(ASCP) WS 11: Troubleshooting Immunohistochemistry: Bonnie Whitaker, HT(ASCP) QIHC WS 12: Microwave Technology.? What is it Good for?.Absolutely Everything: Donna Willis, HT/HTL(ASCP)? WS 13: Personality Types in the Histology Laboratory: Glenda Hood, BS, HT (ASCP) WS 14: New Advances in Immunohistochemistry Staining: Tommy Reese, HT (ASCP)? WS 15: A Pathologist looks at the Passion and Death of Jesus Christ: Kevin McQuaid, M.D. SYMPOSIUMS Symposium #1 What Every Histotechnologist and Pathologist Needs to Know about Technical Immunohistochemistry: Rodney T. Miller, M.D. Symposium #2 Transmission Electron Microscopy: Elma Cortinis, BS, HT (ASCP) Symposium #3 Histochemical Stains for the Evaluation of GI Tract and Liver Disease- What They Are and How They Are Used in Daily Practice: Shari Taylor, M.D. Symposium #4 Immunohistochemistry in the Analysis of Forensic Evidence from a Double Homicide in New Zealand:? The Lundy Case, Rodney T. Miller, M.D.? Symposium #5 Forensic Pathology Overview:? Joseph M. Guileyardo, M.D ? ? From kdwyer3322 <@t> aol.com Thu Mar 13 20:51:21 2008 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Thu Mar 13 20:51:30 2008 Subject: [Histonet] Fwd: Texas Society for Histotechnology Symposium/Convention April 18-20, 2008 In-Reply-To: <8CA538CABCF5B0A-1594-1ED6@FWM-M15.sysops.aol.com> References: <8CA538CABCF5B0A-1594-1ED6@FWM-M15.sysops.aol.com> Message-ID: <8CA538D0960F6B2-1594-1EEE@FWM-M15.sysops.aol.com> -----Original Message----- From: kdwyer3322@aol.com To: HistoNet@pathology.swmed.edu Cc: Veronida@baylorhealth.edu; samuel.jones2@med.va.gov; mhale@carisdx.com Sent: Thu, 13 Mar 2008 7:48 pm Subject: Texas Society for Histotechnology Symposium/Convention April 18-20, 2008 Hi Histonetters! The Texas Society for Histotechnology will be hosting the annual Symposium/Convention, April 18-20, 2008 at the DFW Hilton Lakes, 1800 Hwy 26, Grapevine,Texas.? Golf Tournanment will be hosted on April 17, 2008 at 1:00pm to register for golf contact mhale@carisdx.com .? If you would like a copy of the program or more information please reply to this e-mail. Workshops include: WS 1:? Small Specimen Management (In a Large Volume World): Skip Brown, M.Div., HT (ASCP) WS 2: ?A Review of the Human Cell and an Introduction to Cell Injury: Mark Bailey, MA, HT (ASCP) WS 3: Are You Prepared to Take the HT (ASCP) Registry Exam: Robert Lott, BS., HTL(ASCP) WS 4: Cytology for Histotechnologist: From Tissue to Liquids and Everything in Between: T.W. Crook, M.D. and Thuy Ardaman, M.D. WS 5: Laboratory Safety-What You Need to Know: Jason Burill, Histology Manager WS 6: HQUIP: Freida L. Carson, PhD; Sue E. Lewis, BS, HTL(ASCP) WS 7: Principles of Grossing: Steve Eagle, RS, PA/ASCP eligible? WS 8: Introduction to Immunos: Bonnie Whitaker, HT (ASCP)QIHC WS 9: Is Your Tissue Processor Fighting You? Fight Back: Pam Marcum HT(ASCP) WS 10: LEADERSHIP JAZZ: Developing Alignment & Synchronicity in Lab Operations: Skip Brown, M. Div., HT(ASCP) WS 11: Troubleshooting Immunohistochemistry: Bonnie Whitaker, HT(ASCP) QIHC WS 12: Microwave Technology.? What is it Good for?.Absolutely Everything: Donna Willis, HT/HTL(ASCP)? WS 13: Personality Types in the Histology Laboratory: Glenda Hood, BS, HT (ASCP) WS 14: New Advances in Immunohistochemistry Staining: Tommy Reese, HT (ASCP)? WS 15: A Pathologist looks at the Passion and Death of Jesus Christ: Kevin McQuaid, M.D. SYMPOSIUMS Symposium #1 What Every Histotechnologist and Pathologist Needs to Know about Technical Immunohistochemistry: Rodney T. Miller, M.D. Symposium #2 Transmission Electron Microscopy: Elma Cortinis, BS, HT (ASCP) Symposium #3 Histochemical Stains for the Evaluation of GI Tract and Liver Disease- What They Are and How They Are Used in Daily Practice: Shari Taylor, M.D. Symposium #4 Immunohistochemistry in the Analysis of Forensic Evidence from a Double Homicide in New Zealand:? The Lundy Case, Rodney T. Miller, M.D.? Symposium #5 Forensic Pathology Overview:? Joseph M. Guileyardo, M.D ? ? Supercharge your AIM. Get the AIM toolbar for your browser. From louise.renton <@t> gmail.com Fri Mar 14 02:39:56 2008 From: louise.renton <@t> gmail.com (louise renton) Date: Fri Mar 14 02:40:06 2008 Subject: [Histonet] Lung processing In-Reply-To: <7A885E8FE1C71C488D974EC601FAA69002E0865A@vla-exchn1.cvlnt.vla.gov.uk> References: <7A885E8FE1C71C488D974EC601FAA69002E0865A@vla-exchn1.cvlnt.vla.gov.uk> Message-ID: Are you loading more cassettes into the new processor? are they perhaps packed more tightly than before? - this may affect the effect of vacuum on the "inner" cassettes in relation to those on the outside..... just my 2c worth On 3/13/08, Weaver, Colin wrote: > Hi - everyone we have just moved from routine processing in a VIP 1000 > to a Thermo-Shandon Excelsior. This has been working perfectly except > when processing lung, especially spongy lung. When embedding after > processing wax infiltration appears to be patchy, bubbles come out of > the tissue when pressing the tissue into a mould, after trimming there > are "holes" in the tissue, and sections often crumble because of lack of > wax support. We have vacuumn on everything from first alcohol onwards. > Anyone got any suggestions as to why this is happening? We used to use > Thermo-Shandon Hypercenter XP's and had the same problem with lung, is > it a Thermo thing? > > Colin > Histology Dept > VLA Thirsk > Sunny Yorkshire > Veterinary Laboratories Agency (VLA) > > This email and any attachments is intended for the named recipient > only. > If you have received it in error you have no authority to use, disclose, > store or copy any of its contents and you should destroy it and inform > the sender. > Whilst this email and associated attachments will have been checked > for known viruses whilst within VLA systems we can accept no > responsibility once it has left our systems. > Communications on VLA's computer systems may be monitored and/or > recorded to secure the effective operation of the system and for other > lawful purposes. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From DEllenburg2 <@t> stfrancishealth.org Fri Mar 14 06:41:57 2008 From: DEllenburg2 <@t> stfrancishealth.org (Ellenburg, Deborah) Date: Fri Mar 14 06:42:07 2008 Subject: [Histonet] Productivity Message-ID: Hi Histo Netters, Does anyone have or know of any standards and/or guidelines for measuring productivity in histology? I know that all techs do not work at the same pace but what would be considered a reasonable amount of time to embed 50 blocks, section 50 blocks and etc? Thank you, Deborah Ellenburg, HT (ASCP) Histology Supervisor Bon Secours St. Francis Health System One St. Francis Drive Greenville, SC 29601 864-255-1582 ________________________________________________________________________________________________________________________________ ________________________________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. From bakevictoria <@t> gmail.com Fri Mar 14 07:53:38 2008 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Fri Mar 14 07:53:43 2008 Subject: [Histonet] marker resistant to triton In-Reply-To: References: Message-ID: <4f016b690803140553i748e8239off153b19de75f1b3@mail.gmail.com> Helen, The Securline marker will fade but it does stay on. The only other one I can think of is #2 pencil, last resort a diamond pen etched on the BACK side of the slide at the bottom. I always used an excel grid that I had made up indicating which slot I had placed the slide, then using the diamond pen I would etch "1a", "2a" etc. The number would indicate the row, the letter the slot. It's not a perfect system, but it worked. Vikki On 3/13/08, Helen E Johnson wrote: > > Hi Histonetters, > I am inquiring if there is a permanent slide marker that is resistant > to the chemical triton when doing IHC. > Helen Johnson > (hej01@health.state.ny.us) > > > IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jcline <@t> wchsys.org Fri Mar 14 09:41:16 2008 From: jcline <@t> wchsys.org (Joyce Cline) Date: Fri Mar 14 09:41:29 2008 Subject: [Histonet] CAP ? Message-ID: <000001c885e1$75d1aaf0$1d2a14ac@wchsys.org> How do you handle the question "Is the identity of every specimen maintained through each step of processing and slide preparation? NOTE: An unambiguous system of specimen identification coupled with a legible, sequential cassette and slide labeling system that withstands reagents and stains are essential to fulfill this requirement. The number of blocks processed and the number of slides for each case must be recorded. The bolded statement is what I am curious about. Does everyone use their computer system to keep track of the amount of cassettes and slides? Is anyone interfaced between the computer system with their cassette and slide printers? We have our embedding sheets with the block counts, but I have never kept track of each case slide count. Totals for cases, slides and blocks every month is done along with specials. Do you go back in an add recuts? ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From marktarango <@t> gmail.com Fri Mar 14 10:20:52 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Mar 14 10:21:04 2008 Subject: [Histonet] CAP ? In-Reply-To: <000001c885e1$75d1aaf0$1d2a14ac@wchsys.org> References: <000001c885e1$75d1aaf0$1d2a14ac@wchsys.org> Message-ID: <5b6eb13e0803140820i251c2aa2l35ccb8a4dbdd86a5@mail.gmail.com> I think the best way to do this is to have the pathologist dictate it in each report. "Eleven H&E and three IHC-stained slides were prepared and examined in rendering the diagnosis of this case." ...or something. It lets everyone know what the pathologist actually looked at. On Fri, Mar 14, 2008 at 7:41 AM, Joyce Cline wrote: > How do you handle the question "Is the identity of every specimen > maintained through each step of processing and slide preparation? > NOTE: An unambiguous system of specimen identification coupled with a > legible, sequential cassette and slide labeling system that withstands > reagents and stains are essential to fulfill this requirement. The > number of blocks processed and the number of slides for each case must > be recorded. > > The bolded statement is what I am curious about. Does everyone use their > computer system to keep track of the amount of cassettes and slides? Is > anyone interfaced between the computer system with their cassette and > slide printers? > > We have our embedding sheets with the block counts, but I have never > kept track of each case slide count. Totals for cases, slides and blocks > every month is done along with specials. Do you go back in an add > recuts? > > > ***** CONFIDENTIALITY NOTICE ***** > This message contains confidential information and is intended only for > the individual named. If you are not the named addressee you should not > disseminate, distribute or copy this e-mail. Please notify the sender > immediately by e-mail if you have received this e-mail by mistake and > delete this e-mail from your system. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From CIngles <@t> uwhealth.org Fri Mar 14 10:53:13 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Fri Mar 14 10:53:25 2008 Subject: [Histonet] Tracking Specimens References: Message-ID: <08A0A863637F1349BBFD83A96B27A50A120103@uwhis-xchng3.uwhis.hosp.wisc.edu> Amy: We get lots of specimens from off site that are either mailed or brought by courier. We have each clinic fill out their own log sheet to send along with the specimens. They have to list patient name and info. Plus patient insurance info if they aren't in our system. They also have to list each individual specimen for each patient, and the site the specimen was taken from. This helps us not only to keep patients straight, but lets us know no specimens were 'lost' during transport. The specimen site is so that the correct site for the specimen label can be verified. We used to have a few mix ups a month where we had to contact the clinic to verify what was what because they had mixed up sites and labels somewhere between the bottle label, the order sheet, and the logsheet. It helps eliminate the "wrong site, wrong side" possibilty. If there is a discrepancy we have an error resolution sheet we fill out, then we contact the clinic to get someone to verfiy what is what. We keep the logsheets in a book to document specimen workload. Hope this helps. Claire ________________________________ Dear Histonetters, How are Histology Labs keeping an audit trail to track specimens from the time that they are collected, are picked up from the physicians' offices by couriers, and then delivered to the Lab that performs the histology testing? What kinds of logs are being kept? Is anyone using a barcode system? Do you have an Outreach program? From POWELL_SA <@t> Mercer.edu Fri Mar 14 10:58:49 2008 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Fri Mar 14 11:03:22 2008 Subject: [Histonet] Region III hotel registration has been extended another week Message-ID: <01MSESXA3SNA001BVK@Macon2.Mercer.edu> The Westin Hotel has extended our registration deadline another week. We have exceeded our room block numbers and they are happy to have more attendees register. They have also given the reduced rate for those coming in before Thursday or staying past Saturday 12:00. So you can come early or extend your stay and see more of Atlanta and the surrounding area. Reminder for Region III Hi Guys, March 20th, next Thursday, is the revised deadline for making your reservations for Region III meeting hosted by GSH. The Westin Peachtree Plaza has extended the deadline for registration for the Region III meeting in Atlanta Georgia April 3-5, 2008. Take advantage of the discounted rate of $129 single, double, triple or quad. The phone number to the Westin is 1-404-659-1400 and please state that you are attending the GSH/Region III meeting. Program Registration Deadline to avoid a late fee is March 21st. The program can be downloaded from our website at www.histosearch.com/gsh. Click on the symposium link to get a PDF file of the program. Vendors have a link to their registration form to exhibit at the meeting on the same page. If you have questions Chris Coley, GSH Exhibit Liaison, has contact information is on that form. There has been some confusion about the workshop fees. The $35 registration fee is nonrefundable and payable by everyone. This covers the seminars and Friday lunch, but the workshops, #1 through #5 are $40 each for members, $55 each for Nonmembers (either NSH or GSH) and $25 each for students (requires signature of program director). Workshops 1 and 2 will run concurrently and only one of them can be taken. Number 3 is Saturday morning, no conflicting workshop, only seminars. Workshops 4 and 5 are in the afternoon running concurrently so only one of them can be taken. I hope this clears up any questions you may have. If anyone has further questions please feel to contact me at this email address. Come to Atlanta and join us for a great meeting. Shirley Powell GSH Secretary/Registrar From Susan.Pemberton <@t> bmcjax.com Fri Mar 14 11:17:08 2008 From: Susan.Pemberton <@t> bmcjax.com (Pemberton, Susan) Date: Fri Mar 14 11:17:23 2008 Subject: [Histonet] Leica Slide Printer Message-ID: <4E59E1E7B5A80F448F47424C8A2FDEDB01493DF7@BHEXCHVS02.BH.LOCAL> Our laboratory is a new user of the Leica Slide Printer. We previously used the TBS slide etcher. We are having trouble with our new printer and the vendor suggested that we were using the wrong slides. Has anyone had experience with the slides for this printer? We currently are using Evermark slides without clipped corners. We need multiple colors of slides because of the various hospitals and types of cases our laboratory serves. Thank you, Susan Susan L. Pemberton MS, MT(ASCP)SM,DLM Laboratory Administrative Director . Baptist Health 800 Prudential Drive . Jacksonville, FL 32207 (904) 202-2016 . Fax: (904) 202-2795 ----------------------------------------- NOTICE: This message is confidential, intended for the named recipient(s) and may contain information that is (i) proprietary to the sender, and/or,(ii) privileged, confidential and/or otherwise exempt from disclosure under applicable Florida and federal law, including, but not limited to, privacy standards imposed pursuant to the federal Health insurance Portability and Accountability Act of 1996 ("HIPAA"). Receipt by anyone other than the named recipient(s) is not a waiver of any applicable privilege. Thank you in advance for your compliance with this notice. From Jan.Minshew <@t> leica-microsystems.com Fri Mar 14 11:38:31 2008 From: Jan.Minshew <@t> leica-microsystems.com (Jan.Minshew@leica-microsystems.com) Date: Fri Mar 14 11:53:55 2008 Subject: [Histonet] Leica Slide Printer In-Reply-To: <4E59E1E7B5A80F448F47424C8A2FDEDB01493DF7@BHEXCHVS02.BH.LOCAL> Message-ID: Hi Susan, I just saw your message below that was posted on the Histonet and I'd be happy to help you with recommendations for slides to be used in the Leica Microsystems IP S. Please feel free to contact me at your convenience using the information below. Best wishes, Jan Minshew, HT/HTL(ASCP) Marketing Manager Leica Microsystems Biosystems Division 2345 Waukegan Road Bannockburn, IL 60015 800.248.0123 Toll Free 847.405.7051 Direct 847.405.6560 Fax www.leica-microsystems.com Click Here for this month's special offers! "Pemberton, Susan" Sent by: cc histonet-bounces@ "Livingston, Glenda S" lists.utsouthwest ern.edu Subject [Histonet] Leica Slide Printer 03/14/2008 11:17 AM Our laboratory is a new user of the Leica Slide Printer. We previously used the TBS slide etcher. We are having trouble with our new printer and the vendor suggested that we were using the wrong slides. Has anyone had experience with the slides for this printer? We currently are using Evermark slides without clipped corners. We need multiple colors of slides because of the various hospitals and types of cases our laboratory serves. Thank you, Susan Susan L. Pemberton MS, MT(ASCP)SM,DLM Laboratory Administrative Director . Baptist Health 800 Prudential Drive . Jacksonville, FL 32207 (904) 202-2016 . Fax: (904) 202-2795 ----------------------------------------- NOTICE: This message is confidential, intended for the named recipient(s) and may contain information that is (i) proprietary to the sender, and/or,(ii) privileged, confidential and/or otherwise exempt from disclosure under applicable Florida and federal law, including, but not limited to, privacy standards imposed pursuant to the federal Health insurance Portability and Accountability Act of 1996 ("HIPAA"). Receipt by anyone other than the named recipient(s) is not a waiver of any applicable privilege. Thank you in advance for your compliance with this notice. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From tjasper <@t> copc.net Fri Mar 14 12:10:46 2008 From: tjasper <@t> copc.net (Thomas Jasper) Date: Fri Mar 14 12:10:52 2008 Subject: [Histonet] Leica Slide Printer References: <4E59E1E7B5A80F448F47424C8A2FDEDB01493DF7@BHEXCHVS02.BH.LOCAL> Message-ID: <90354A475B420441B2A0396E5008D4965E2074@copc-sbs.COPC.local> Hey Susan, We used a Leica slide printer at the medical center I was previously at. I believe having clipped corners is pretty much a necessity with the printer. Yes, it will work without them but you are prone to a lot more trouble with the unit. We used to get multi-colored, cut corner slides for use with it. I don't recall the vendor off the top of my head, but I'm sure someone on this list, or your Leica rep could direct you to a source. Our IT guy set it up to read the LIS work list. You could then command (through a PC) the slide printer to create slides needed for cases as they were accessioned during the day. Good Luck, Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pemberton, Susan Sent: Friday, March 14, 2008 9:17 AM To: histonet@lists.utsouthwestern.edu Cc: Livingston, Glenda S Subject: [Histonet] Leica Slide Printer Our laboratory is a new user of the Leica Slide Printer. We previously used the TBS slide etcher. We are having trouble with our new printer and the vendor suggested that we were using the wrong slides. Has anyone had experience with the slides for this printer? We currently are using Evermark slides without clipped corners. We need multiple colors of slides because of the various hospitals and types of cases our laboratory serves. Thank you, Susan Susan L. Pemberton MS, MT(ASCP)SM,DLM Laboratory Administrative Director . Baptist Health 800 Prudential Drive . Jacksonville, FL 32207 (904) 202-2016 . Fax: (904) 202-2795 ----------------------------------------- NOTICE: This message is confidential, intended for the named recipient(s) and may contain information that is (i) proprietary to the sender, and/or,(ii) privileged, confidential and/or otherwise exempt from disclosure under applicable Florida and federal law, including, but not limited to, privacy standards imposed pursuant to the federal Health insurance Portability and Accountability Act of 1996 ("HIPAA"). Receipt by anyone other than the named recipient(s) is not a waiver of any applicable privilege. Thank you in advance for your compliance with this notice. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From crochieresteve <@t> aol.com Fri Mar 14 12:31:49 2008 From: crochieresteve <@t> aol.com (crochieresteve@aol.com) Date: Fri Mar 14 12:32:00 2008 Subject: [Histonet] VIP 1000 repairs Message-ID: <8CA54106B92B236-1648-DEC@WEBMAIL-DF03.sysops.aol.com> Does anyone know who can repair an old VIP 1000? Sakura no longer has parts. Someone near Western Mass. would be preferable. Thanks From mark.webber <@t> nuigalway.ie Fri Mar 14 12:43:57 2008 From: mark.webber <@t> nuigalway.ie (Webber, Mark) Date: Fri Mar 14 12:45:09 2008 Subject: [Histonet] Removing DPX coverslips can you leave in xylene for several days without affecting tissue Message-ID: <6B017AD2AE2F6F489087FC986588136B045B7BCB@EVS1.ac.nuigalway.ie> Have done DAB and then coverslipped with DPX I have been required to counterstain with haematoxylin. Can the coverslips be removed by soaking in Xylene. For reasons out of my control the sections may be in Xylene for several days Firstly will this affect the tissue? Secondly what would I need to do to counterstain (rehydrate/dehydrate etc??) as have never had to do this Thanks Mark From alonso.martinezcanabal <@t> utoronto.ca Fri Mar 14 12:55:25 2008 From: alonso.martinezcanabal <@t> utoronto.ca (Alonso Martinez-Canabal) Date: Fri Mar 14 12:55:55 2008 Subject: FW: [Histonet] Removing DPX coverslips can you leave in xylene forseveral days without affecting tissue Message-ID: <15982ACDF64C4B38815A8E235EF73697@Astrocito> Just soak them in xylene, but you have to be patient, usually the coverslips take like three days to fall off alone. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webber, Mark Sent: March-14-08 1:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Removing DPX coverslips can you leave in xylene forseveral days without affecting tissue Have done DAB and then coverslipped with DPX I have been required to counterstain with haematoxylin. Can the coverslips be removed by soaking in Xylene. For reasons out of my control the sections may be in Xylene for several days Firstly will this affect the tissue? Secondly what would I need to do to counterstain (rehydrate/dehydrate etc??) as have never had to do this Thanks Mark _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmarcum <@t> vet.upenn.edu Fri Mar 14 13:05:02 2008 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Fri Mar 14 13:05:09 2008 Subject: [Histonet] Removing DPX coverslips can you leave in xylene for several days without affecting tissue In-Reply-To: <6B017AD2AE2F6F489087FC986588136B045B7BCB@EVS1.ac.nuigalway .ie> References: <6B017AD2AE2F6F489087FC986588136B045B7BCB@EVS1.ac.nuigalway.ie> Message-ID: <6.2.5.6.2.20080314140431.01c84a80@vet.upenn.edu> Yes! You can remove by soaking until they come off with no problem in xylene. Pam Marcum At 01:43 PM 3/14/2008, Webber, Mark wrote: >Have done DAB and then coverslipped with DPX I have been required to >counterstain with haematoxylin. Can the coverslips be removed by soaking >in Xylene. > > > >For reasons out of my control the sections may be in Xylene for several >days > > > >Firstly will this affect the tissue? > >Secondly what would I need to do to counterstain (rehydrate/dehydrate >etc??) as have never had to do this > > > >Thanks > >Mark > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From Allison_Scott <@t> hchd.tmc.edu Fri Mar 14 13:13:56 2008 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Fri Mar 14 13:16:23 2008 Subject: [Histonet] VVG with weigerts Message-ID: <1872B4A455B7974391609AD8034C79FC8BD40D@LBEXCH01.hchd.local> Hello to everyone. Happy Friday. Does anyone have a procedure for vvg using weigerts hematoxylin instead of the standard Hematoxylin that is in the procedure. Any help will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 From Shawna.Thomas <@t> Integris-Health.com Fri Mar 14 13:25:04 2008 From: Shawna.Thomas <@t> Integris-Health.com (Thomas, Shawna G.) Date: Fri Mar 14 13:25:16 2008 Subject: [Histonet] Nita Searcy Message-ID: <0AF4F8EA51200F49ADC32E6E353F024307B9E4D7@EXCHANGE2-OKC.corp.integris-health.com> Nita, If you are out there, would you please reply? I have a question I thought you might have an answer for from the "good ole days" at Integris. Shawna Baker, MBA, PA(ASCP) Pathologists' Assistant AmeriPath Oklahoma @ ISMC Pathology (405)644-6146 phone (405)636-7518 fax From mari.ann.mailhiot <@t> leica-microsystems.com Fri Mar 14 13:40:12 2008 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Fri Mar 14 13:40:17 2008 Subject: [Histonet] Leica Slide Printer In-Reply-To: <4E59E1E7B5A80F448F47424C8A2FDEDB01493DF7@BHEXCHVS02.BH.LOCAL> Message-ID: Hi Susan I will send you a copy of the slides and manuafactures of the slides that can be used on the IPS. Best Regards Mari Ann Mailhiot BA HT ASCP Application Specialist/Trainer Leica Microsystems Biosystems Division Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com "Pemberton, Susan" Sent by: cc histonet-bounces@ "Livingston, Glenda S" lists.utsouthwest ern.edu Subject [Histonet] Leica Slide Printer 03/14/2008 11:17 AM Our laboratory is a new user of the Leica Slide Printer. We previously used the TBS slide etcher. We are having trouble with our new printer and the vendor suggested that we were using the wrong slides. Has anyone had experience with the slides for this printer? We currently are using Evermark slides without clipped corners. We need multiple colors of slides because of the various hospitals and types of cases our laboratory serves. Thank you, Susan Susan L. Pemberton MS, MT(ASCP)SM,DLM Laboratory Administrative Director . Baptist Health 800 Prudential Drive . Jacksonville, FL 32207 (904) 202-2016 . Fax: (904) 202-2795 ----------------------------------------- NOTICE: This message is confidential, intended for the named recipient(s) and may contain information that is (i) proprietary to the sender, and/or,(ii) privileged, confidential and/or otherwise exempt from disclosure under applicable Florida and federal law, including, but not limited to, privacy standards imposed pursuant to the federal Health insurance Portability and Accountability Act of 1996 ("HIPAA"). Receipt by anyone other than the named recipient(s) is not a waiver of any applicable privilege. Thank you in advance for your compliance with this notice. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From Paul <@t> Firnschild.com Fri Mar 14 17:59:08 2008 From: Paul <@t> Firnschild.com (Paul Firnschild) Date: Fri Mar 14 16:59:21 2008 Subject: [Histonet] VIP 1000 repairs References: <8CA54106B92B236-1648-DEC@WEBMAIL-DF03.sysops.aol.com> Message-ID: <054101c88627$036c39d0$cf977e18@PhelpsDodge> Hi Steve, Have tools, parts, knowledge and references. I can fly a load of parts up there as checked baggage, then pick them up from the (Albany?) airport and drive to you in a rental car. I'd be happy to help. Just let me know. Paul Atlanta, GA Paul M. Firnschild QA Support Services, Inc. 404.291.3715 (t) 419.818.3618 (f) email: Paul@Firnschild.com ----- Original Message ----- From: To: Sent: Friday, March 14, 2008 12:31 PM Subject: [Histonet] VIP 1000 repairs > Does anyone know who can repair an old VIP 1000? Sakura no longer has parts. Someone near Western Mass. would be preferable. Thanks ----- Original Message ----- From: To: Sent: Friday, March 14, 2008 12:31 PM Subject: [Histonet] VIP 1000 repairs > Does anyone know who can repair an old VIP 1000? Sakura no longer has parts. Someone near Western Mass. would be preferable. Thanks > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From gdeville <@t> deltapathology.com Fri Mar 14 22:48:28 2008 From: gdeville <@t> deltapathology.com (Gwen Deville) Date: Fri Mar 14 22:48:40 2008 Subject: [Histonet] Hacker Linear stainer Message-ID: <000c01c8864f$6ea89720$d61da8c0@dpgdomain.com> Does anyone have a good H&E procedure for the Hacker Linear stainer. What type /brand of hematoxylin and eosin works best for consistent staining for this type of stainer? Gwen Deville, Histology Supv. Delta Pathology, Mid-Louisiana Box 30113, 211 Fourth Street Alexandria, LA 71301 Work: (318)473-3943 / (318) 473-3180 Email: gdeville@deltapathology.com From gdeville <@t> deltapathology.com Fri Mar 14 22:57:45 2008 From: gdeville <@t> deltapathology.com (Gwen Deville) Date: Fri Mar 14 22:57:56 2008 Subject: [Histonet] Cryo console Message-ID: <001701c88650$bb037210$d61da8c0@dpgdomain.com> Does anyone know where I could purchase a used cryo console for a TissueTek or Microm embedding unit? Gwen Deville, Histology Supv. Delta Pathology, Mid-Louisiana Box 30113, 211 Fourth Street Alexandria, LA 71301 Work: (318)473-3943 / (318) 473-3180 Mobile: (318) 729-4203 Pager: (318) 427-5444 Email: gdeville@deltapathology.com NOTICE: This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissenmination, distribution, forwarding, printing, or copying of the email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. From gdeville <@t> deltapathology.com Fri Mar 14 22:59:56 2008 From: gdeville <@t> deltapathology.com (Gwen Deville) Date: Fri Mar 14 23:00:11 2008 Subject: [Histonet] (no subject) Message-ID: <001c01c88651$08e864e0$d61da8c0@dpgdomain.com> Does anyone know where I can purchase a used cryo console. Gwen Deville, Histology Supv. Delta Pathology, Mid-Louisiana Box 30113, 211 Fourth Street Alexandria, LA 71301 Work: (318)473-3943 / (318) 473-3180 Email: gdeville@deltapathology.com From marjoh3 <@t> telus.net Sat Mar 15 13:26:36 2008 From: marjoh3 <@t> telus.net (Marilyn Johnson) Date: Sat Mar 15 13:26:38 2008 Subject: [Histonet] Endogenous Peroxide Treatment - Immunohistochemistry Message-ID: <009001c886ca$1ab7fb80$6501a8c0@VALUED20606295> Hi Histonetters, For routine tissues, I use 3% hydrogen peroxide for 10 mins. to remove RBC's. Presently, I am trying to stain liver tissue, which stores more of the blood components. There seems to be background staining that masks blood cells and vessels. I've tried using 5% hydrogen peroxide solution for 10 mins. and there is no improvement. Are there other solutions that can be used instead of hydrogen peroxide? Any replies would be greatly appreciated. Thank you in advance. Marilyn Johnson Alberta Agriculture Food and Safety Division Edmonton, Alberta, Canada. From imilos <@t> cellmarque.com Sat Mar 15 13:29:08 2008 From: imilos <@t> cellmarque.com (Isaac Milos) Date: Sat Mar 15 13:40:34 2008 Subject: [Histonet] Endogenous Peroxide Treatment - Immunohistochemistry In-Reply-To: <009001c886ca$1ab7fb80$6501a8c0@VALUED20606295> References: <009001c886ca$1ab7fb80$6501a8c0@VALUED20606295> Message-ID: <7F2A2AE306CE254DB7279E86A51A740657BE92@CMROCEX01.cellmarque.local> Hi Marilyn, If you are running your IHC stains with an HRP detection kit with a biotinylated secondary antibody, endogenous biotin within the tissue can cause background staining to occur. Often times, you'll see endogenous biotin in a tissue that is particularly bloody, such as liver, kidney, or brain. You can use an avidin/biotin block before secondary antibody is applied to account for this. You can find this block through many different companies - mine (Cell Marque) offers a good version. The part number is CMX222 and it can be ordered at 1-800-665-7284. Happy staining! Isaac -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marilyn Johnson Sent: Saturday, March 15, 2008 11:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Endogenous Peroxide Treatment - Immunohistochemistry Hi Histonetters, For routine tissues, I use 3% hydrogen peroxide for 10 mins. to remove RBC's. Presently, I am trying to stain liver tissue, which stores more of the blood components. There seems to be background staining that masks blood cells and vessels. I've tried using 5% hydrogen peroxide solution for 10 mins. and there is no improvement. Are there other solutions that can be used instead of hydrogen peroxide? Any replies would be greatly appreciated. Thank you in advance. Marilyn Johnson Alberta Agriculture Food and Safety Division Edmonton, Alberta, Canada. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From detmar <@t> mshri.on.ca Sat Mar 15 13:43:33 2008 From: detmar <@t> mshri.on.ca (Jacqui Detmar) Date: Sat Mar 15 13:43:53 2008 Subject: [Histonet] Endogenous Peroxide Treatment - Immunohistochemistry In-Reply-To: <009001c886ca$1ab7fb80$6501a8c0@VALUED20606295> References: <009001c886ca$1ab7fb80$6501a8c0@VALUED20606295> Message-ID: Hi Marilyn. I work on mouse placenta - also very bloody - and I find that I get the best peroxidase quenching when I put the slides in 3% H202 in methanol for 20-30 minutes. Also, for most of my antibodies, I can do the quenching step after the biotinylated secondary antibody, but BEFORE adding the ABC solution. Just make sure the slides are well washed after quenching. Hope this helps, Jacqui Jacqui Detmar, Post-doctoral Fellow Samuel Lunenfeld Research Institute, room 876 Mount Sinai Hospital 600 University Avenue Toronto, ON, Canada M5G 1X5 phone: 416-586-4800 x2451/x2290 fax: 416-586-8588 email: detmar@mshri.on.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marilyn Johnson Sent: Saturday, March 15, 2008 2:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Endogenous Peroxide Treatment - Immunohistochemistry Hi Histonetters, For routine tissues, I use 3% hydrogen peroxide for 10 mins. to remove RBC's. Presently, I am trying to stain liver tissue, which stores more of the blood components. There seems to be background staining that masks blood cells and vessels. I've tried using 5% hydrogen peroxide solution for 10 mins. and there is no improvement. Are there other solutions that can be used instead of hydrogen peroxide? Any replies would be greatly appreciated. Thank you in advance. Marilyn Johnson Alberta Agriculture Food and Safety Division Edmonton, Alberta, Canada. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pcprabuin <@t> gmail.com Sun Mar 16 01:38:26 2008 From: pcprabuin <@t> gmail.com (prabu prabu) Date: Sun Mar 16 01:38:32 2008 Subject: [Histonet] H&E staining protocol for Leica ST 4040 Message-ID: <1c7fdf750803152338n618f3736v9a5bf219534fd380@mail.gmail.com> Dear all, Does any one have H&E staining protocol for Leica ST 4040 linear stainer.Help me in this regard. Thanking you From anh2006 <@t> med.cornell.edu Sun Mar 16 10:48:23 2008 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Sun Mar 16 10:48:33 2008 Subject: [Histonet] Endogenous Peroxide Treatment - Immunohistochemistry In-Reply-To: <7F2A2AE306CE254DB7279E86A51A740657BE92@CMROCEX01.cellmarque.local> References: <009001c886ca$1ab7fb80$6501a8c0@VALUED20606295> <7F2A2AE306CE254DB7279E86A51A740657BE92@CMROCEX01.cellmarque.local> Message-ID: Dear Marilyn, I agree with Isaac, that the likely culprit when staining liver is biotin background. I don't know whether that is or is not the issue in your case until we knew more about your protocol. For my protocols, I use 3% H202 in H20 for 10 min RT and it blocks endogenous peroxidase in liver very well for most applications (the endogenous biotin still needs to be blocked with a kit). However, if there is extramedullary hematopoiesis or a large infiltration of neutrophils you may see WBC associated endogenous peroxidase which is nearly impossible to block with standard methods. For this we use glucose oxidase blocking method. I have an excellent protocol from Gayle Callis I have used for years I can share if you like. In fact, Gayle may even respond to this email with the protocol herself also. Cheers, Andrea >Hi Marilyn, > >If you are running your IHC stains with an HRP detection kit with a >biotinylated secondary antibody, endogenous biotin within the tissue can >cause background staining to occur. Often times, you'll see endogenous >biotin in a tissue that is particularly bloody, such as liver, kidney, >or brain. > >You can use an avidin/biotin block before secondary antibody is applied >to account for this. You can find this block through many different >companies - mine (Cell Marque) offers a good version. The part number >is CMX222 and it can be ordered at 1-800-665-7284. > >Happy staining! > >Isaac > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marilyn >Johnson >Sent: Saturday, March 15, 2008 11:27 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Endogenous Peroxide Treatment - Immunohistochemistry > >Hi Histonetters, >For routine tissues, I use 3% hydrogen peroxide for 10 mins. to remove >RBC's. >Presently, I am trying to stain liver tissue, which stores more of the >blood components. >There seems to be background staining that masks blood cells and >vessels. >I've tried using 5% hydrogen peroxide solution for 10 mins. and there is >no improvement. >Are there other solutions that can be used instead of hydrogen peroxide? >Any replies would be greatly appreciated. >Thank you in advance. > >Marilyn Johnson >Alberta Agriculture >Food and Safety Division >Edmonton, Alberta, Canada. -- From tammy <@t> surgicalpathlabs.com Sun Mar 16 19:45:49 2008 From: tammy <@t> surgicalpathlabs.com (Tammy de Leon) Date: Sun Mar 16 19:45:50 2008 Subject: [Histonet] Histo jobs In-Reply-To: <6.2.5.6.2.20080314140431.01c84a80@vet.upenn.edu> Message-ID: Good evening. I am an HR Mgr in Pinellas Park, Florida and have several histology jobs open. Do you recommend a job board? This is the job info I need to post: If you're looking for a friendly environment, a new state-of-the-art LEED certified facility and an employer of choice, SPL is the place for you!! We are currently looking for qualified Histotechnicians! Candidates should be an HTL or HT (ASCP) or equivalent. Primary responsibilities include on-site frozen sections including mobile laboratory units. SPL is CAP accredited, offers competitive pay, a comprehensive benefits package inc., Medical, Dental, LTD, Life, Retirement Plan & Supplemental Insurance. Please forward resume to Tammy de Leon tammy@surgicalpathlabs.com Surgical Pathology Laboratory 800-304-1066 7641 66th Street N Pinellas Park, Florida 33781 www.surgicalpathlabs.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Friday, March 14, 2008 2:05 PM To: Webber, Mark; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Removing DPX coverslips can you leave in xylenefor several days without affecting tissue Yes! You can remove by soaking until they come off with no problem in xylene. Pam Marcum At 01:43 PM 3/14/2008, Webber, Mark wrote: >Have done DAB and then coverslipped with DPX I have been required to >counterstain with haematoxylin. Can the coverslips be removed by soaking >in Xylene. > > > >For reasons out of my control the sections may be in Xylene for several >days > > > >Firstly will this affect the tissue? > >Secondly what would I need to do to counterstain (rehydrate/dehydrate >etc??) as have never had to do this > > > >Thanks > >Mark > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Michelle.Perrins <@t> uct.ac.za Mon Mar 17 02:14:09 2008 From: Michelle.Perrins <@t> uct.ac.za (Michelle Perrins) Date: Mon Mar 17 02:14:35 2008 Subject: [Histonet] ratio Message-ID: <47DE3660.A704.0070.0@uct.ac.za> Hi Is there a set ratio between medical technologists and pathologists in histopathology labs. Michelle Michelle Perrins Chief Medical Technologist Forensic Pathology Services Division Forensic Medicine Faculty Health Sciences University of Cape Town tel: +27 21 406 6001 fax: +27 21 448 1249 Email: Michelle.Perrins@uct.ac.za From Melker.Goransson <@t> astrazeneca.com Mon Mar 17 02:36:04 2008 From: Melker.Goransson <@t> astrazeneca.com (=?iso-8859-1?Q?G=F6ransson=2C_Melker?=) Date: Mon Mar 17 02:36:13 2008 Subject: [Histonet] Strong dye for adipose tissue cell membranes Message-ID: <64A531C5BF0A1F4DA4D02849B43FEF370111FA1C@SEMLRDEMBX01.rd.astrazeneca.net> Hi all, I need a strong dye to stain cell membranes in 5?m sections of paraffin embedded adipose tissue. Prolonged Htx-E is not enough in order to perform computer based size measurements using the software i got. I am quite unexperienced in the histology field, is there a strong stain suitable for staining of cell membranes ( nuclei may stain as well ) ?? Thank You/ Melker From relia1 <@t> earthlink.net Mon Mar 17 07:59:23 2008 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Mar 17 07:59:30 2008 Subject: [Histonet] RELIA Job Alert - 3/17/08 Message-ID: Hello Histonetters I hope everyone had a great weekend. I just wanted to post some new opportunities that I am excited about. I have several full time permanent day shift positions in the following locations: Harrisonburg, VA Arlington, VA Washington, DC Lancaster, PA Los Angeles, CA All of these clients offer excellent compensation, benefits and in most cases relocation or sign-on bonuses. If you or anyone you know might be interested please feel free to shoot me an e-mail at relia1@earthlink.net or give me a call toll free at 866-607-3542. Happy St. Patricks Day!! Thank You! Pam M. Barker President Relia 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll Free: (866)607-3542 e-mail: relia1@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia From MLafrini <@t> csmlab.com Mon Mar 17 07:53:53 2008 From: MLafrini <@t> csmlab.com (Michael LaFriniere) Date: Mon Mar 17 08:40:28 2008 Subject: [Histonet] Grossing Tech In-Reply-To: <05CAE76AB5D5ED409864C6DD86F13349238CEC6C@pbpsflexch02.pbp.local> References: <05CAE76AB5D5ED409864C6DD86F13349238CEC6C@pbpsflexch02.pbp.local> Message-ID: <47DE3194.588C.00AF.0@csmlab.com> Judy, If the grossing tech is at $50.00 per hour that is the going rate in some parts of the country....my experience has been in the east and south...most 88305's nets $34-75 per specimen on the technical aspect of the billing for the laboratory dependant on the payor mix... Michael R. LaFriniere Executive Director Cytology Services of Maryland (CSM) 301-206-2555 ext 27 301-206-2595 fax michael.lafriniere@csmlab.com >>> On 3/11/2008 at 9:45 AM, in message <05CAE76AB5D5ED409864C6DD86F13349238CEC6C@pbpsflexch02.pbp.local>, Judy Collins wrote: Our grossing techs usually gross up to 50 specimens per hour (most are 88305) so at your suggested rate, they would be paid $400.00 per day. That would be $50.00 per hour. That seems unreasonable. Judy Collins Palm Beach Pathology _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bliven.laura <@t> marshfieldclinic.org Mon Mar 17 08:50:25 2008 From: bliven.laura <@t> marshfieldclinic.org (Bliven, Laura) Date: Mon Mar 17 08:50:35 2008 Subject: [Histonet] Antibody for HBsAg Message-ID: <200803171350.m2HDoRVp032661@spamfilt.mfldclin.edu> I'm looking for a good Hepatitis B Surface Antigen antibody. Must be ASR. I know recently some companies changed from IVD to ASR or RUO. In our clinical setting it must be at least ASR. Also, I understand that it matters whether we use a polyclonal or monoclonal antibody. Should I assume that the antibody must be polyclonal for this virus as some others viruses require it, so it's not too specific and may miss positive cases? So, the questions are: which antibody for HBsAg that's ASR (no RUO labeled) and polyclonal or monoclonal? Antibody consistency from the company is important as we use concentrated antibodies. Thanks, Laura Bliven Histology Lab/IHC Marshfield Laboratories 1000 N. Oak Ave. Marshfield, WI 54449 From PMonfils <@t> Lifespan.org Mon Mar 17 08:56:02 2008 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Mon Mar 17 08:56:10 2008 Subject: [Histonet] Strong dye for adipose tissue cell membranes In-Reply-To: <64A531C5BF0A1F4DA4D02849B43FEF370111FA1C@SEMLRDEMBX01.rd.astrazeneca.net> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273D2C@LSRIEXCH1.lsmaster.lifespan.org> A 0.5% solution of basic fuchsin or crystal violet should do it. These wouldn't work on cells with a lot of cytoplasm because the cytoplasm would stain just as dark as the membranes. But on fat, where there isn't much there except the membrane and the nucleus, it should work well. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of G?ransson, Melker > Sent: Monday, March 17, 2008 2:36 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Strong dye for adipose tissue cell membranes > > Hi all, > I need a strong dye to stain cell membranes in 5?m sections of paraffin embedded adipose tissue. Prolonged Htx-E is not enough in order to perform computer based size measurements using the software i got. I am quite unexperienced in the histology field, is there a strong stain suitable for staining of cell membranes ( nuclei may stain as well ) ?? > Thank You/ > Melker > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From LSebree <@t> uwhealth.org Mon Mar 17 09:05:57 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Mon Mar 17 09:06:06 2008 Subject: [Histonet] Antibody for HBsAg In-Reply-To: <200803171350.m2HDoRVp032661@spamfilt.mfldclin.edu> Message-ID: Hi Laura, Always a pleasure to help out a fellow Wisconsinite. We use Hep. B surface from Thermo Scientific (manufactured by NeoMarkers for Lab Vision Corp.), cat. # MS-314-S0 (0.1 ml), or -S1 (0.5 ml), or -S (1.0 ml) for the supernatant concentrate. We use it at a 1:15 dilution on our Ventana stainers with no pretreatment. This antibody is an ASR. Their number is: 1(800)828-1628. Hope this helps, Linda Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bliven, Laura Sent: Monday, March 17, 2008 8:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antibody for HBsAg I'm looking for a good Hepatitis B Surface Antigen antibody. Must be ASR. I know recently some companies changed from IVD to ASR or RUO. In our clinical setting it must be at least ASR. Also, I understand that it matters whether we use a polyclonal or monoclonal antibody. Should I assume that the antibody must be polyclonal for this virus as some others viruses require it, so it's not too specific and may miss positive cases? So, the questions are: which antibody for HBsAg that's ASR (no RUO labeled) and polyclonal or monoclonal? Antibody consistency from the company is important as we use concentrated antibodies. Thanks, Laura Bliven Histology Lab/IHC Marshfield Laboratories 1000 N. Oak Ave. Marshfield, WI 54449 From Vickroy.Jim <@t> mhsil.com Mon Mar 17 09:45:52 2008 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Mon Mar 17 09:49:03 2008 Subject: [Histonet] ELECTRONIC REQUISITIONS WITH CERNER CLASSIC OR MILLENIUM Message-ID: <24A4826E8EF0964D86BC5317306F58A5053732DC85@mmc-mail.ad.mhsil.com> I am working with our information systems staff in setting up an electronic ordering system for surgical specimens. Most of our tissue specimens come with a written req and we would like to try to set up something in the order system so that an electronic req. can be printed with each specimen. In addition if it was put into the system then we would have a mechanism to track the ordering. Right now the floors and hospital departments can put a "message" into the system but this doesn't come up on a log anywhere. In addition it does not print a req. All of the clinical lab has a system for electronic orders but as usual surgical pathology is lagging behind. I know there are pathology groups out there thinking, why not use a different system like copath, etc. There are others that are in the real world that know we have to use the system that the entire hospital has purchased. We are currently using Cerner Classic Pathnet and will change to Cerner Millenium. The hospital uses Cerner Millenium with Powerchart, etc. The guys from IT have asked me to check with colleagues to see how others have set up this system. Has anybody set this up in their lab and do you have any contacts that I can send my IT geeks to? I am sure we are behind in the computer electronic world. Thanks for your help. Jim Vickroy Memorial Medical Center Springfield, IL. This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From rjbuesa <@t> yahoo.com Mon Mar 17 10:09:32 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 17 10:09:41 2008 Subject: [Histonet] ratio In-Reply-To: <47DE3660.A704.0070.0@uct.ac.za> Message-ID: <997993.12620.qm@web65707.mail.ac4.yahoo.com> Michelle: The ratio HT/PT depends on the type of laboratory and fluctuates between 0.2 to 5.0, with an average of 1.4 +/- 0.9 and a media value of 1.3 If interested I can send you the graph for this relation. Ren? J. Michelle Perrins wrote: Hi Is there a set ratio between medical technologists and pathologists in histopathology labs. Michelle Michelle Perrins Chief Medical Technologist Forensic Pathology Services Division Forensic Medicine Faculty Health Sciences University of Cape Town tel: +27 21 406 6001 fax: +27 21 448 1249 Email: Michelle.Perrins@uct.ac.za _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From victor <@t> pathology.washington.edu Mon Mar 17 10:15:08 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Mon Mar 17 10:15:18 2008 Subject: [Histonet] ELECTRONIC REQUISITIONS WITH CERNER CLASSIC OR MILLENIUM In-Reply-To: <24A4826E8EF0964D86BC5317306F58A5053732DC85@mmc-mail.ad.mhsil.com> References: <24A4826E8EF0964D86BC5317306F58A5053732DC85@mmc-mail.ad.mhsil.com> Message-ID: <47DE8AFC.4020708@pathology.washington.edu> Jim, Here is our situation. We use PowerPath from IMPAC for our LIS. The hospital has Cerner for the EMR. Our satellite clinics use EPIC. PowerPath has a module for electronic orders and we are currently receiving orders from EPIC. I don't work on the hospital side of things and don't know what Cerner can do. The electronic ordering makes accessioning very easy. Enter the order number in PowerPath and all the patient demographics are loaded, referring physician, brief clinic history and specimen. The specimen usually has to be modified to bring in one of our panels, but it definitely saves time. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Vickroy, Jim wrote: > I am working with our information systems staff in setting up an electronic ordering system for surgical specimens. Most of our tissue specimens come with a written req and we would like to try to set up something in the order system so that an electronic req. can be printed with each specimen. In addition if it was put into the system then we would have a mechanism to track the ordering. Right now the floors and hospital departments can put a "message" into the system but this doesn't come up on a log anywhere. In addition it does not print a req. All of the clinical lab has a system for electronic orders but as usual surgical pathology is lagging behind. > > I know there are pathology groups out there thinking, why not use a different system like copath, etc. There are others that are in the real world that know we have to use the system that the entire hospital has purchased. We are currently using Cerner Classic Pathnet and will change to Cerner Millenium. The hospital uses Cerner Millenium with Powerchart, etc. > > The guys from IT have asked me to check with colleagues to see how others have set up this system. Has anybody set this up in their lab and do you have any contacts that I can send my IT geeks to? I am sure we are behind in the computer electronic world. > > Thanks for your help. > > > Jim Vickroy > Memorial Medical Center > Springfield, IL. > > > > > > This message (including any attachments) contains confidential information intended for a > > specific individual and purpose, and is protected by law. If you are not the intended recipient, > > you should delete this message. Any disclosure, copying, or distribution of this message, or the > > taking of any action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ksecrest <@t> hsc.wvu.edu Mon Mar 17 10:17:56 2008 From: ksecrest <@t> hsc.wvu.edu (Kimberly Secrest) Date: Mon Mar 17 10:18:38 2008 Subject: [Histonet] Histology Atlas Message-ID: <47DE536C.C068.0078.0@hsc.wvu.edu> Can anyone recommend a really good histology atlas. It will be used for teaching new students. Thanks Kim Kimberly Secrest, HTL, QIHC Instructor Department of Pathology West Virginia University 304-293-7628 From JMacDonald <@t> mtsac.edu Mon Mar 17 10:39:12 2008 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Mar 17 10:39:23 2008 Subject: [Histonet] Histology Atlas In-Reply-To: <47DE536C.C068.0078.0@hsc.wvu.edu> Message-ID: The two that we use for our histology course are: Wheater?s Functional HISTOLOGY, a Text and Color Atlas, Young, B., Heath, J.W., Churchill Livingstone. ISBN: 0-443-05612-9 Color Atlas of Histology, Gartner, Leslie, Hiatt, James, Lippincott Williams & Wilkins. ISBN: 0-7817-2585-2 I have looked at quite a few and I found these to be the most user friendly for beginning students. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Kimberly Secrest" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/17/2008 08:20 AM To cc Subject [Histonet] Histology Atlas Can anyone recommend a really good histology atlas. It will be used for teaching new students. Thanks Kim Kimberly Secrest, HTL, QIHC Instructor Department of Pathology West Virginia University 304-293-7628 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jerry.santiago <@t> jax.ufl.edu Mon Mar 17 10:37:57 2008 From: jerry.santiago <@t> jax.ufl.edu (Santiago, Jerry) Date: Mon Mar 17 10:39:29 2008 Subject: [Histonet] Florida Society for Histotechnology Annual Meeting Message-ID: <816DC61E1730E843A0BCF4CCFA1EFCF30148F1EF@jaxmail.umc.ufl.edu> Dear Histonetters, The Program for the Florida Society for Histotechnology Meeting is now completed. The meeting is scheduled for May 15-18, 2008 at the Bahia Mar Beach Resort in Fort Lauderdale. You may register online at www.fshmeeting.com or visit our website at www.fshgroup.org and click on the 2008 Meeting link. Hope to see in Fort Lauderdale. From mcauliff <@t> umdnj.edu Mon Mar 17 10:50:52 2008 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Mon Mar 17 10:50:53 2008 Subject: [Histonet] Histology Atlas In-Reply-To: <47DE536C.C068.0078.0@hsc.wvu.edu> References: <47DE536C.C068.0078.0@hsc.wvu.edu> Message-ID: <47DE935C.8000803@umdnj.edu> I second the choices Jennifer mentioned. We use both of those. Geoff Kimberly Secrest wrote: > Can anyone recommend a really good histology atlas. It will be used for teaching new students. > Thanks > Kim > > Kimberly Secrest, HTL, QIHC > Instructor > Department of Pathology > West Virginia University > 304-293-7628 > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From Jon.St.Onge <@t> dako.com Mon Mar 17 11:18:30 2008 From: Jon.St.Onge <@t> dako.com (Jon St. Onge) Date: Mon Mar 17 11:18:44 2008 Subject: [Histonet] Hematoxylin Usage Message-ID: <8B07D141BCDE434285DC12B3290E3FB301E09A97@exbackca.caus.dako.net> Good Morning Histonetters, We are trying to extend our Hematoxylin usage and would appreciate your feedback- Currently we stain using 25 slide holders into a staining bath with ~200mL of Mayers Hematoxylin. We change the bath every 100 slides or every week. Thank you and Happy Monday, Dako North America Jon Henry St. Onge Histology Jon.St.Onge@dako.com (805)566-6655 ext. 5303 From liz <@t> premierlab.com Mon Mar 17 12:53:39 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Mon Mar 17 12:53:44 2008 Subject: [Histonet] collagen I IHC for human only Message-ID: Hello Everyone Is anyone out there aware of a collagen I antibody that works in FFPE tissue samples that works on human tissue but will not cross react with porcine tissue? I have searched a bit and really have come up with nothing so far. Thanks in advance. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 From liz <@t> premierlab.com Mon Mar 17 13:52:39 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Mon Mar 17 13:52:46 2008 Subject: [Histonet] ammoniacal silver for histones Message-ID: I'm trying to find a more detailed procedure for this technique. It is referenced back to Black and Ansley from 1964 but the reference is a bit vague on the actual procedure. I'm actually looking for a protocol that would work on paraffin sections, but I have been unable to come up with anything do far. Any help would be appreciated and thanks in advance. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 From esther.peters <@t> verizon.net Mon Mar 17 14:06:45 2008 From: esther.peters <@t> verizon.net (Esther Peters) Date: Mon Mar 17 14:07:39 2008 Subject: [Histonet] Need Olympus CUT4060 Standard Object Clamp Message-ID: <47DEC145.1030503@verizon.net> We need the Standard Object Clamp (I believe this is part CUT1010) for an Olympus CUT4060 microtome we recently received as a donation. Can someone please direct me to where I might be able to buy one (Google search was not helpful)? Thank you! Esther Peters, Ph.D. George Mason University epeters2@gmu.edu From gayle.callis <@t> bresnan.net Mon Mar 17 15:53:38 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Mon Mar 17 15:53:36 2008 Subject: [Histonet] complete endogenous peroxidae blocking in liver and other tissues Message-ID: <000c01c88870$fa3bb1f0$6401a8c0@DHXTS541> There is an endogenous peroxidase blocking method using glucose oxidase + glucose which works very well for frozen sections and also FFPE tissues. It is an enzymatic chemical reaction that produces a slow, steady rate of hydrogen peroxide to remove endogenous peroxidase and pseudoperoxidaes without damaging mophology in frozen sections. The sodium azide contributes to the peroxidase blocking. It has been used successfully by people having trouble removing these peroxidases from FFPE tissues. Do not try to buy the glucose from Sigma as they discontinued this particular glucose. Glucose Oxidase Peroxidase Block (GLUOX) beta D(+) glucose, 97% pure from ICN Biomedicals Corp #100953 0.180g glucose oxidase (Sigma G6641) 0.005g sodium azide 0.0065g Dulbeccos PBS (Sigma, no Mg or Ca added) 50 ml We made up a large stock of the DPBS with sodium azide to avoid weighing out sodium azide in such small amounts, and referred to this buffer as the GLUOX buffer. DO NOT PREHEAT THE BLOCKING SOLUTION BEFORE USE. Protocol 1. Immerse deparaffinized section in the GLUOX mixture and incubate for 30 min to 1 hour. One hour may be advisable for FFPE tissue, in the literature and for frozen sections, time was 30 min - 1 hr. 2. Rinse 3X in DPBS and proceed with IHC method. Andrew SM, Jasani B. An improved method for the inhibition of endogenous peroxidase non-deleterious to lymphocyte surface markers. Application to immunoperoxidae studies on eosinophil-rich tissue preparations. Histochem J 19:426-430, 1987. Good luck, Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT From jcox90 <@t> yahoo.com Tue Mar 18 09:39:03 2008 From: jcox90 <@t> yahoo.com (Jill Cox) Date: Tue Mar 18 09:39:12 2008 Subject: [Histonet] Looking for Travel or Temp Histology work in Florida Message-ID: <936238.27821.qm@web56804.mail.re3.yahoo.com> Hi Netters, Does anyone know of a facility in Florida looking for Travel or Temp Histologist. I am Florida Licensed and can work now. Please respond to this email for further details. Thanks Jill Cox HT (ASCP) From sbreeden <@t> nmda.nmsu.edu Tue Mar 18 10:25:11 2008 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Mar 18 10:25:25 2008 Subject: [Histonet] NSH Summer Symposium Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E61F9@nmdamailsvr.nmda.ad.nmsu.edu> The NSH has announced the first-ever "regional" symposium to be held on June 20-21 in Albuquerque! Complete information can be found (and downloaded) from the NSH website (www.nsh.org ) along with a registration form. As President of the New Mexico Society for Histology, I'd like to issue this personal invitation to come and visit Albuquerque for this Summer Symposium. It is our honor to help host this unique meeting and to be part of this premier NSH offering. Check the NSH website for program and schedule, then plan to spend some extra time here visiting Old Town (great shopping!), the Botanical Park and Aquarium (yes, we have water here in New Mexico!), walk along the Rio Grande (not the same water as in the Aquarium or you couldn't see the fish!), ride the tram to Sandia Peak for a spectacular view of that mighty, muddy Rio Grande, and decide your own answer to our Official State Question: "Red or Green?" (referring to your preference between red chile and green chile). Please come and join us! If it SNOWS this time, I will not only be VERY surprised - I'll spring for breakfast burritos! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From MMcCOY <@t> lakelandregional.org Tue Mar 18 10:34:03 2008 From: MMcCOY <@t> lakelandregional.org (Mary McCoy) Date: Tue Mar 18 10:32:09 2008 Subject: [Histonet] CPT Code for FNA Message-ID: <20080318T113403Z_C27C00150000@lakelandregional.org> Can anyone tell me the correct CPT code to use for billing the technical component of preparing slides and screening for FNAs. Thanks, Mary McCoy HTL(ASCP) Supervisor of Pathology Services Lakeland Regional Health System St. Joseph MI 49098 (269) 982-4891 mmccoy@lakelandregional.org From AWeiss <@t> shorememorial.org Tue Mar 18 12:19:54 2008 From: AWeiss <@t> shorememorial.org (AWeiss@shorememorial.org) Date: Tue Mar 18 12:20:09 2008 Subject: [Histonet] Re: Histonet Digest, Vol 52, Issue 27 In-Reply-To: <20080318170213.4742B3B3072@smhex1.shorememorial.org> Message-ID: We use the CPT codes 88173 88172 for FNA's Andrea J Weiss BST CT (ASCP) Cytotechnologist 609 653 3577 Ext 4907 aweiss@shorememorial.org From SaClark <@t> ameripath.com Tue Mar 18 12:39:59 2008 From: SaClark <@t> ameripath.com (Clark, Sam) Date: Tue Mar 18 12:40:04 2008 Subject: [Histonet] 2 Histology positions- San Antonio, Texas Message-ID: We have 2 positions available at Ameripath in San Antonio, Texas. If interested please send resume to sastaffing@ameripath.com. You can also view our ad at http://nsh.org. Thanks, Sam Sam Clark HT, ASCP Histology Manager Ameripath South Texas ************************************************************************ ******* THIS ELECTRONIC MAIL MESSAGE AND ANY ATTACHMENT IS CONFIDENTIAL AND MAY CONTAIN PRIVILEGED INFORMATION INTENDED ONLY FOR THE USE OF THE INDIVIDUAL OR INDIVIDUALS NAMED ABOVE. If the reader is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please reply to the sender to notify us of the error and delete the original message. ************************************************************************ ******* From sbreeden <@t> nmda.nmsu.edu Tue Mar 18 13:23:38 2008 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Mar 18 13:23:52 2008 Subject: [Histonet] OT: Tuesday Tasty Tidbit Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E61FF@nmdamailsvr.nmda.ad.nmsu.edu> Okay, so it's not the Friday Hour of Fuming but this will do. Without giving away my source, my boss received an email this morning asking, "Does anyone monitor wind speed in their histo labs?". My boss replied that we don't because our lab is INDOORS. This has been a source of great amusement all morning - I know what the person is asking, but it just struck me as funny! Adios Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From Robinsoc <@t> mercyhealth.com Tue Mar 18 14:10:37 2008 From: Robinsoc <@t> mercyhealth.com (Cynthia Robinson) Date: Tue Mar 18 14:10:59 2008 Subject: [Histonet] OT: Tuesday Tasty Tidbit In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E61FF@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B017E61FF@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <47DFCD5D.59BC.00AF.0@mercyhealth.com> Perhaps they meant to say number of air exchanges but wind speed is definitely more creative. >>> "Breeden, Sara" 3/18/2008 1:23 PM >>> Okay, so it's not the Friday Hour of Fuming but this will do. Without giving away my source, my boss received an email this morning asking, "Does anyone monitor wind speed in their histo labs?". My boss replied that we don't because our lab is INDOORS. This has been a source of great amusement all morning - I know what the person is asking, but it just struck me as funny! Adios Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Tue Mar 18 14:22:20 2008 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Tue Mar 18 14:22:32 2008 Subject: [Histonet] OT: Tuesday Tasty Tidbit Message-ID: <405030.65642.qm@web50106.mail.re2.yahoo.com> We don't measure wind speed. I sometimes need a speed limit on people walking by too fast and their wake blows the ribbon I just cut out of my hands. :) Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-759-3953 contact@excaliburpathology.com From rjbuesa <@t> yahoo.com Tue Mar 18 14:56:00 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 18 14:56:03 2008 Subject: [Histonet] OT: Tuesday Tasty Tidbit In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E61FF@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <659592.12660.qm@web65706.mail.ac4.yahoo.com> Sara: There is nothing to flame about. It is just a misunderstanding, what you HAVE to measure is the wind FLOW in your fume hoods. There are small vanes that you place at the entrance, where the air flows the most, and one a week (or a day if you wish) you check the vane to determine if the air is flowing and at what RATE (or speed if you will). I have a vane in each of my 4 fumes hoods, and had a log for that determination. Ren? J. "Breeden, Sara" wrote: Okay, so it's not the Friday Hour of Fuming but this will do. Without giving away my source, my boss received an email this morning asking,"Does anyone monitor wind speed in their histo labs?". My boss replied that we don't because our lab is INDOORS. This has been a source of great amusement all morning - I know what the person is asking, but it just struck me as funny! Adios Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From b-frederick <@t> northwestern.edu Tue Mar 18 15:21:50 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue Mar 18 15:22:17 2008 Subject: [Histonet] OT: Tuesday Tasty Tidbit In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E61FF@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <000001c88935$b819cdf0$d00f7ca5@lurie.northwestern.edu> I have an air vent(*!!>>???) over my head and it frequently ruins a beautiful ribbon as do people that create a breeze as they walk by knowing full well you have ribbon between the microtome and waterbath!!!!! Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Tuesday, March 18, 2008 1:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OT: Tuesday Tasty Tidbit Okay, so it's not the Friday Hour of Fuming but this will do. Without giving away my source, my boss received an email this morning asking, "Does anyone monitor wind speed in their histo labs?". My boss replied that we don't because our lab is INDOORS. This has been a source of great amusement all morning - I know what the person is asking, but it just struck me as funny! Adios Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From juditw <@t> u.washington.edu Tue Mar 18 15:40:42 2008 From: juditw <@t> u.washington.edu (Judith L. Williams) Date: Tue Mar 18 15:40:51 2008 Subject: [Histonet] OT: Tuesday Tasty Tidbit In-Reply-To: <659592.12660.qm@web65706.mail.ac4.yahoo.com> Message-ID: Tell the person interested in wind speed that they will need a horizontally fixed pitot tube - as in the kind affixed to the wings of airplanes to measure wind speed! that should get them going!!!! you can find out about pitot tubes in Google website- NASA has a good description with diagrams should they want them (haha!) still chuckling over this one, Judy in Washington On Tue, 18 Mar 2008, Rene J Buesa wrote: > Sara: > There is nothing to flame about. It is just a misunderstanding, what you HAVE to measure is the wind FLOW in your fume hoods. There are small vanes that you place at the entrance, where the air flows the most, and one a week (or a day if you wish) you check the vane to determine if the air is flowing and at what RATE (or speed if you will). > I have a vane in each of my 4 fumes hoods, and had a log for that determination. > Ren? J. > > "Breeden, Sara" wrote: > Okay, so it's not the Friday Hour of Fuming but this will do. Without > giving away my source, my boss received an email this morning asking,"Does anyone monitor wind speed in their histo labs?". My boss replied > that we don't because our lab is INDOORS. This has been a source of > great amusement all morning - I know what the person is asking, but it > just struck me as funny! Adios > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > --------------------------------- > Never miss a thing. Make Yahoo your homepage. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Judith Williams, PhD, HT(ASCP) Research Scientist Department of Comparative Medicine University of Washington Seattle, WA 98195 From marktarango <@t> gmail.com Tue Mar 18 15:50:44 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Tue Mar 18 15:50:49 2008 Subject: [Histonet] OT: Tuesday Tasty Tidbit In-Reply-To: References: <659592.12660.qm@web65706.mail.ac4.yahoo.com> Message-ID: <5b6eb13e0803181350v258e41fkdad84f0c4203af1e@mail.gmail.com> I dunno, when someone comes running by and I've got a ribbon in the air... I'd call it wind speed. There are a few people I can think of who have a strong naturally occuring tornado-like wind surrounding them. Reminds me of the tasmanian devil. I'm sure it's something truly unmeasurable lol On Tue, Mar 18, 2008 at 1:40 PM, Judith L. Williams wrote: > Tell the person interested in wind speed that they will need a > horizontally fixed pitot tube - as in the kind affixed to the wings of > airplanes to measure wind speed! that should get them going!!!! you can find > out about pitot tubes in Google website- NASA has a good description with > diagrams should they want them (haha!) > still chuckling over this one, > Judy in Washington > > > On Tue, 18 Mar 2008, Rene J Buesa wrote: > > > Sara: > > There is nothing to flame about. It is just a misunderstanding, what you > HAVE to measure is the wind FLOW in your fume hoods. There are small vanes > that you place at the entrance, where the air flows the most, and one a week > (or a day if you wish) you check the vane to determine if the air is flowing > and at what RATE (or speed if you will). > > I have a vane in each of my 4 fumes hoods, and had a log for that > determination. > > Ren? J. > > > > "Breeden, Sara" wrote: > > Okay, so it's not the Friday Hour of Fuming but this will do. Without > > giving away my source, my boss received an email this morning > asking,"Does anyone monitor wind speed in their histo labs?". My boss > replied > > that we don't because our lab is INDOORS. This has been a source of > > great amusement all morning - I know what the person is asking, but it > > just struck me as funny! Adios > > > > > > > > Sally Breeden, HT(ASCP) > > > > NM Dept. of Agriculture > > > > Veterinary Diagnostic Services > > > > PO Box 4700 > > > > Albuquerque, NM 87106 > > > > 505-841-2576 > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > --------------------------------- > > Never miss a thing. Make Yahoo your homepage. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > Judith Williams, PhD, HT(ASCP) > Research Scientist > Department of Comparative Medicine > University of Washington > Seattle, WA 98195 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From AnthonyH <@t> chw.edu.au Tue Mar 18 17:08:25 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Mar 18 17:09:21 2008 Subject: [Histonet] OT: Tuesday Tasty Tidbit Message-ID: I know someone who seems to have lots of wind. Can I attach one of these "horizontally fixed pitot tubes" to him or would a "vane" do the job? Seems like a job for a proctologist! Or should I just put him in the fume hood! I don't know whether I should post this - ah what the heck!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Judith L. Williams Sent: Wednesday, 19 March 2008 7:41 AM To: Rene J Buesa Cc: histonet@lists.utsouthwestern.edu; Breeden, Sara Subject: Re: [Histonet] OT: Tuesday Tasty Tidbit Tell the person interested in wind speed that they will need a horizontally fixed pitot tube - as in the kind affixed to the wings of airplanes to measure wind speed! that should get them going!!!! you can find out about pitot tubes in Google website- NASA has a good description with diagrams should they want them (haha!) still chuckling over this one, Judy in Washington On Tue, 18 Mar 2008, Rene J Buesa wrote: > Sara: > There is nothing to flame about. It is just a misunderstanding, what > you HAVE to measure is the wind FLOW in your fume hoods. There are > small vanes that you place at the entrance, where the air flows the > most, and one a week (or a day if you wish) you check the vane to > determine if the air is flowing and at what RATE (or speed if you > will). I have a vane in each of my 4 fumes hoods, and had a log for > that determination. Ren? J. > > "Breeden, Sara" wrote: > Okay, so it's not the Friday Hour of Fuming but this will do. > Without giving away my source, my boss received an email this morning > asking,"Does anyone monitor wind speed in their histo labs?". My boss > replied that we don't because our lab is INDOORS. This has been a > source of great amusement all morning - I know what the person is > asking, but it just struck me as funny! Adios > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > --------------------------------- > Never miss a thing. Make Yahoo your homepage. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Judith Williams, PhD, HT(ASCP) Research Scientist Department of Comparative Medicine University of Washington Seattle, WA 98195 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From scoop <@t> mail.nih.gov Tue Mar 18 18:35:22 2008 From: scoop <@t> mail.nih.gov (Sharon) Date: Tue Mar 18 17:36:09 2008 Subject: [Histonet] question about immunofluorescence staining in mouse spleen Message-ID: Dear experts, My boss has asked me about immunofluorescence staining in mouse spleen: How do you get rid of fluorescent background in mouse spleen? I suggested ammonia ethanol quenching which I know works in bone marrow. Is there a better method? Also, my boss claims that the macrophages have autofluorescence (formalin fixed paraffin embedded tissue). I always thought the rbcs would be the autofluorescent cells. Do the macrophages have autofluorescence? Finally, is it reasonable to do frozen sections of mouse spleen? I thought frozen sections fixed in ice cold acetone or acetone-methanol might solve the problem, but I was told that frozen sections would be much to thick to get any good immunofluorescent pictures on. Thanks for any info and suggestions. Unknowledgeably yours, Sharon From Samuel_Perry <@t> DFCI.HARVARD.EDU Tue Mar 18 18:27:11 2008 From: Samuel_Perry <@t> DFCI.HARVARD.EDU (Perry, Samuel) Date: Tue Mar 18 18:27:17 2008 Subject: [Histonet] Barrier/Pap Pen Message-ID: Dear Histoneters, I am having problems with my pap pen. I have been using the pap pen from VWR called the "Liquid Blocker super pap pen." It's terrible; every time I wash in Tween 20/PBS it loses it's ability to make a hydrophobic barrier, which mean I'm constantly patching the barrier before adding my primary, secondary, and developing steps. I found one made by Ambion cat# 15500G which works great but they only sell these in a 150$ LCM kit. Does anyone know who Ambion gets theirs from? Or does anyone have recommendations of a pap pen they like the best? Thanks for your help! Sam Perry Research Technician Dana Farber Cancer Institute Boston MA 02115 The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From anh2006 <@t> med.cornell.edu Tue Mar 18 18:38:55 2008 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Tue Mar 18 18:39:02 2008 Subject: [Histonet] question about immunofluorescence staining in mouse spleen In-Reply-To: References: Message-ID: Hi Sharon, For immunofluorescence, frozen sections are the easiest way to go with the best results. Acetone, methanol or a even a mix of acetone:ethanol are wonderful ways to fix frozen sections of murine spleen for IF. A brief 2-4% PFA fixation on fresh frozen splenic sections works also but the PFA will increase the "autofluorescence". It will depend on the antibody which fixation works best ... Do you have access to a confocal or similar type system (one with apotome or deconvolution software etc.)? If so, you can do 30-100 um sections with beautiful results. For regular epifluorescence, we use 8-12 um sections with excellent results. Let me know if you need further information/specifics, Andrea >Dear experts, > >My boss has asked me about immunofluorescence staining in mouse >spleen: How do you get rid of fluorescent background in mouse >spleen? I suggested ammonia ethanol quenching which I know works in >bone marrow. Is there a better method? Also, my boss claims that >the macrophages have autofluorescence (formalin fixed paraffin >embedded tissue). I always thought the rbcs would be the >autofluorescent cells. Do the macrophages have autofluorescence? >Finally, is it reasonable to do frozen sections of mouse spleen? I >thought frozen sections fixed in ice cold acetone or >acetone-methanol might solve the problem, but I was told that frozen >sections would be much to thick to get any good immunofluorescent >pictures on. > >Thanks for any info and suggestions. > >Unknowledgeably yours, >Sharon -- From magicchessa <@t> yahoo.com Tue Mar 18 19:03:19 2008 From: magicchessa <@t> yahoo.com (Malissa Snyder) Date: Tue Mar 18 19:03:22 2008 Subject: [Histonet] internship help Message-ID: <607977.31223.qm@web50208.mail.re2.yahoo.com> Hello everyone, I was wondering if any of you would be able to help steer me in the right direction. I am currently a student in a histotechnology program and am looking for a place within 1-2 hours of Tampa Bay Florida to do my clinical training. Does anyone know any hospitals in that area? Thanks, Malissa --------------------------------- Never miss a thing. Make Yahoo your homepage. From gayle.callis <@t> bresnan.net Tue Mar 18 19:11:07 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Tue Mar 18 19:11:10 2008 Subject: [Histonet] Barrier/Pap Pen References: Message-ID: <004501c88955$ba8ac060$6401a8c0@DHXTS541> Try Immedge pens from Vector. Cheaper, two in a set. When you use this pen, and maybe even your pens, shake the begollys out of the pen to redistribute the ingredients per advice from Vector. We vortex ours before using - on highest speed, and make sure the goo is coming out by touching tip to paper. Too much goo is a disaster. If you are making the barrier after the slide surface is wet, make sure it is dry where you want the barrier. We make straight line barriers rather than circles to blot from a corner - easier. The Tween may be causing some of the problem so making a mark on slide that has not been immersed in buffer with Tween may help. Beware, the section could dry though. Gayle Callis HTL/HT/MT(ASCP) Bozeman MT ----- Original Message ----- From: "Perry, Samuel" To: Sent: Tuesday, March 18, 2008 5:27 PM Subject: [Histonet] Barrier/Pap Pen Dear Histoneters, I am having problems with my pap pen. I have been using the pap pen from VWR called the "Liquid Blocker super pap pen." It's terrible; every time I wash in Tween 20/PBS it loses it's ability to make a hydrophobic barrier, which mean I'm constantly patching the barrier before adding my primary, secondary, and developing steps. I found one made by Ambion cat# 15500G which works great but they only sell these in a 150$ LCM kit. Does anyone know who Ambion gets theirs from? Or does anyone have recommendations of a pap pen they like the best? Thanks for your help! Sam Perry Research Technician Dana Farber Cancer Institute Boston MA 02115 The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Tue Mar 18 19:38:32 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Tue Mar 18 19:38:29 2008 Subject: [Histonet] question about immunofluorescence staining in mousespleen References: Message-ID: <005101c88959$8fb13870$6401a8c0@DHXTS541> ----- Original Message ----- From: "Sharon" To: Sent: Tuesday, March 18, 2008 5:35 PM Subject: [Histonet] question about immunofluorescence staining in mousespleen > Dear experts, > > My boss has asked me about immunofluorescence staining in mouse spleen: > How do you get rid of fluorescent background in mouse spleen? Use fresh snap frozen tissues (not cryostat freezing, but true snap freezing) and cut at -17C on a cryostat, pick up on Plus charge, air dry, fix with cold acetone or if doing CD markers, 25% absolute ethanol/75% acetone at RT for 5 minutes but go directly to buffer after this fixative. Then careful normal serum blocking and also clever use of primary antibodies, and secondaries that are F(ab')2 frag of IgG adsorbed to mouse tissue. Jackson has excellent secondaries. We also use biotinylated primary antibodies and come back with Molecular Probes Streapavidin Alexa conjugates, 488 or 594. Have to do avidin/biotin blocking though. No autofluorescence other than tissue components that do autofluoresce. I suggested ammonia ethanol quenching which I know works in bone marrow. Is there a better method? *A better method for FFPE tissues? There are ways, but not always successful. Also, my boss claims that the macrophages have autofluorescence (formalin fixed paraffin embedded tissue). *And a lot of other tissue components too. Aldehydes induce autofluorescence, and it is hard to remove. Go to the IHCWorld website and read up on how autofluorescence can be removed and also a wonderful tutorial on fluorescent staining (link to Wright State?). We do not do murine CD immunosfluorescent staining on formalin fixed tissues. There are some ways to get around this too if CD work is not needed. Formalin fixation, no more than 8 hours, paraffin sections, treat sections with 100 mM glycine in PBS or TBS pH 7.4, 20 minutes, then use polymer kit with alk phos, and DAKO's permanent red, underdeveloped a very light for fluorescence work. Also, there is a lot of information in Histonet archives - have fun searching. I always thought the rbcs would be the autofluorescent cells. Do the macrophages have autofluorescence? Finally, is it reasonable to do frozen sections of mouse spleen? I thought frozen sections fixed in ice cold acetone or acetone-methanol might solve the problem, but I was told that frozen sections would be much to thick to get any good immunofluorescent pictures on. We have no problems cutting 4 um frozen sections (5 um is preferred) of mouse spleen from fresh snap frozen tissues as long as the tissue is equilibrated to a cryostat cutting temperature of -17C. Do not come from a cold bar to immediate sectioning, or the tissue will be crunchy. Thymus can be cut at 3 um, and if careful, spleen could be too. We do double IFA staining on 5 um FS of mouse spleen, can do triple, without any problems. There are autofluroescent tissue components but the fluorophores are so bright, the other dimmer components are not a factor. What you were told was probably because they lacked the technics to do this properly and without success. So go for it! Good luck Gayle Callis HTL/HT/MT(ASCP) Bozemant MT > > Thanks for any info and suggestions. > > Unknowledgeably yours, > Sharon > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hbeard27 <@t> hotmail.com Tue Mar 18 19:45:40 2008 From: hbeard27 <@t> hotmail.com (Helen Beard) Date: Tue Mar 18 19:45:48 2008 Subject: [Histonet] paraformaldehyde autofluorescence In-Reply-To: References: Message-ID: We have been stirring pfa overnight @RT to dissolve, avoiding heat, as one of our researchers believes heating to 60-65C causes autofluorescence in tissue. Has anyone had this problem with autofluorescence? I would prefer to heat and add NaOH. Also what are thoughts on using fresh, frozen 4%pfa? Thanks in advance Helen Beard Adelaide South Australia _________________________________________________________________ Connect and share in new ways with Windows Live. http://www.windowslive.com/share.html?ocid=TXT_TAGHM_Wave2_sharelife_012008 From gayle.callis <@t> bresnan.net Wed Mar 19 09:49:33 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Wed Mar 19 09:49:30 2008 Subject: [Histonet] paraformaldehyde autofluorescence References: Message-ID: <000b01c889d0$722a0b20$6401a8c0@DHXTS541> Helen, It is not the heat used to dissolve paraformaldehyde that causes autofluorescence, it is caused by aldehyde induced autofluorescence when the PFA crosslinks proteins. There are places to buy PFA in liquid form to avoid having to weigh and dissolve. We never bother to add NaOH if we heat, and we also buy our paraformaldehyde from Sigma. We always heat our PFA but never exceeding 60C. It will go into solution around 56C, with stirring in approx 15 minutes or so. I suggest you (and the researcher) read about on autofluorescence on the IHCWorld website. There is a superb discussion on both fluorescence and autofluorescence there. Gayle Callis HTL/HT/MT(ASCP) Bozeman MT ----- Original Message ----- From: "Helen Beard" To: Sent: Tuesday, March 18, 2008 6:45 PM Subject: [Histonet] paraformaldehyde autofluorescence We have been stirring pfa overnight @RT to dissolve, avoiding heat, as one of our researchers believes heating to 60-65C causes autofluorescence in tissue. Has anyone had this problem with autofluorescence? I would prefer to heat and add NaOH. Also what are thoughts on using fresh, frozen 4%pfa? Thanks in advance Helen Beard Adelaide South Australia _________________________________________________________________ Connect and share in new ways with Windows Live. http://www.windowslive.com/share.html?ocid=TXT_TAGHM_Wave2_sharelife_012008_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Wed Mar 19 10:08:27 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Mar 19 10:08:40 2008 Subject: [Histonet] paraformaldehyde autofluorescence In-Reply-To: <000b01c889d0$722a0b20$6401a8c0@DHXTS541> References: <000b01c889d0$722a0b20$6401a8c0@DHXTS541> Message-ID: It is not the heat used to dissolve paraformaldehyde that causes > autofluorescence, it is caused by aldehyde induced autofluorescence when the > PFA crosslinks proteins. There are places to buy PFA in liquid form to > avoid having to weigh and dissolve. We never bother to add NaOH if we heat, > and we also buy our paraformaldehyde from Sigma. > > We always heat our PFA but never exceeding 60C. It will go into solution > around 56C, with stirring in approx 15 minutes or so. We have two protocols for making 4% PFA and I always wondered why one called for a temperature of 65C and the other just about boiling water when adding the PFA. Why would it matter which temperature is used to make the solution if, in the end, the PFA is dissolved? Does heating PFA about 65C cause damage to the solution? Emily -- People aren't like chocolates. People are bastards. Bastards with bastard coating and bastard filling. From williamstewart.pathology <@t> gmail.com Wed Mar 19 10:16:44 2008 From: williamstewart.pathology <@t> gmail.com (William Stewart) Date: Wed Mar 19 10:16:53 2008 Subject: [Histonet] IPEX Stains worked up on VENTANA BENCHMARK (MLH1; MUC6; PARV; Uroplakin3) Message-ID: <81a8916f0803190816w379c00b6s165fb074be86385c@mail.gmail.com> Does anyone have protocols worked up on the Ventana Benchmark for the following stains? MLH1 MUC6 PARV Albumin Uroplakin3 We have had great success automating the process with most antibodies but these are a few that have struggled with and our running manually. All protocols and suggestions would be GREATLY appreciated! Thank you! Bill *William R. Stewart *Department of Pathology Manager, Anatomic Pathology University of Pittsburgh Medical Centers Shadyside Hospital Tel: 412-623-3281 Pager: 412-958-4171 *stewartwr@upmc.edu <*mailto:stewartwr@upmc.edu*>* From greenjumpyone <@t> hotmail.com Wed Mar 19 10:17:15 2008 From: greenjumpyone <@t> hotmail.com (Green JumpyOne) Date: Wed Mar 19 10:17:22 2008 Subject: [Histonet] Formalin Spill Containment Protocol Message-ID: We are currently we are using the product Aldex to absorb and neutralize our formalin and our formalin spills. I have been asked to create a new protocol for containing formalin spills of 1 gallon or less (greater than one gallon and we call the FD). I was hoping that I would not have to recreate the wheel and that perhaps someone out there has a much more complete protocol (than what I have!) they would be willing to share? THANKS! for any help you can provide. Michelle _________________________________________________________________ Enter the Zune-A-Day Giveaway for your chance to win ? day after day after day http://www.windowslive-hotmail.com/ZuneADay/?locale=en-US&ocid=TXT_TAGLM_Mobile_Zune_V1 From tammy <@t> surgicalpathlabs.com Wed Mar 19 11:42:51 2008 From: tammy <@t> surgicalpathlabs.com (Tammy de Leon) Date: Wed Mar 19 11:42:58 2008 Subject: [Histonet] Job openings In-Reply-To: <81a8916f0803190816w379c00b6s165fb074be86385c@mail.gmail.com> Message-ID: I've had several people ask for more info on Surgical Pathology Laboratories and our job openings. This attachment has more info than what was originally posted. Please contact me if you'd like more info. Thanks!! Tammy R. de Leon Surgical Pathology Laboratories Human Resources Officer 7641 66th Street N Suite C Pinellas Park, Fl 33781 Office Phone - 727-545-2339 Fax - 727-545-1644 From tammy <@t> surgicalpathlabs.com Wed Mar 19 12:07:12 2008 From: tammy <@t> surgicalpathlabs.com (Tammy de Leon) Date: Wed Mar 19 12:07:17 2008 Subject: [Histonet] Job openings In-Reply-To: Message-ID: I apologize for the prior email; here is the info from the attachment: Histotechnologist, HT/HTL ASCP Preferred (several openings) Job Title: Histotechnologist, HT/HTL ASCP Preferred Company Name: SPL - Surgical Pathology Laboratories Location: Pinellas Park, FL, USA Job Type: Full Time Min Experience: 1 to 3 years Certification: HT/HTL (ASCP) Preferred Contact Name: Tammy de Leon Phone: 727 545 2339 Email: tammy@surgicalpathlabs.com Fax: 727 545 1644 Description If you're looking for a friendly environment, a new state of the art LEED facility and an employer of choice, SPL is the place for you!! We are currently looking for qualified Histotechnicians! We offer flexible schedule opportunities!! 5 day work weeks or 4 ten hour days allowing for LONG WEEKENDS and more family time!!!!! Candidates should be an HTL or HT (ASCP) or equivalent. Primary responsibilities include on site frozen sections including mobile laboratory units. We offer varied tasks and flexibility with no quotas! Company Profile Surgical Pathology Laboratories is Florida's largest provider of frozen section coverage for surgery centers and doctor's offices. CLIA and Florida licensed, Surgical Pathology is proud to be a College of American Pathologists accredited laboratory. We have been providing frozen section coverage for 10 years. On-site and mobile laboratory units are utilized. We have outgrown our current laboratory space and our new lab is now under construction. It will be the first LEED certified private lab, primarily solar powered, in Pinellas county. Surgical Pathology Laboratories has participated in the training of histotechnologists, Moh's surgeons and medical students. Our staff of ASCP certified histotechnologists have helped train Moh's technicians and offered back-up services for those offices. We also provide QC and slide review as well as diagnosis and retention of tissues for more poorly differentiated squamous cell carcinoma, soft tissue tumors, among others for Moh's surgeons. For those surgeons unable to retain tissue as we do for 10 years, we also provide this service. Surgical Pathology Laboratories also provides slide preparation for individual doctors, clinics and medical examiner offices. Our staff is well qualified and turns out an outstanding product. Providers should contact Surgical Pathology Laboratories for frozen section coverage, slide preparation and related services by calling 800-304-1066. COME GROW WITH US!!! SPL is CAP accredited, offers competitive pay, a comprehensive benefits package inc., Medical, Dental, Life, LTD, Retirement Plan & Supplemental Insurance. You can also take advantage of all that Tampa Bay has to offer - beautiful Gulf beaches, family-friendly attractions, professional sports teams and cultural events. Please forward resume to Tammy de Leon tammy@surgicalpathlabs.com Surgical Pathology Laboratory 800-304-1066 7641 66th Street N Su C Pinellas Park, Florida 33781 www.surgicalpathlabs.com We offer varied tasks and flexibility with no quotas! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tammy de Leon Sent: Wednesday, March 19, 2008 12:43 PM To: 'Histonet' Subject: [Histonet] Job openings I've had several people ask for more info on Surgical Pathology Laboratories and our job openings. This attachment has more info than what was originally posted. Please contact me if you'd like more info. Thanks!! Tammy R. de Leon Surgical Pathology Laboratories Human Resources Officer 7641 66th Street N Suite C Pinellas Park, Fl 33781 Office Phone - 727-545-2339 Fax - 727-545-1644 From histoinfo <@t> comcast.net Wed Mar 19 12:14:18 2008 From: histoinfo <@t> comcast.net (histoinfo@comcast.net) Date: Wed Mar 19 12:14:28 2008 Subject: [Histonet] Kidney Bx question Message-ID: <031920081714.26571.47E149EA00055701000067CB221202078401000207019B9C0708@comcast.net> We have been having some trouble with the inner surface of the tubules looking torn look to the tissue. We hand process all our kidney bx's with the times as follows. 1-formalin 15 minutes 2-50% ethanol 15 minutes 3-70% ethanol 15 minutes 4-80% ethanol 15 minutes 5-95% ethanol 15 minutes 6-100% ethanol 15 minutes 7-100% ethanol 15 minutes 9-xylene 15 minutes 10-xylene 15 minutes Parraffin x2, 20 minutes each. They are then cut at 1 micron for PAS & JONES and 2 microns for H&E & TRICHROME. We are trying to determine if this tearing is an artifact from processing, collection or some other factor we haven't thought of. Has anyone else come across this or a similar problem. All thoughts and suggestions welcome. Jennifer Saunders HT (ASCP) From Terry.Marshall <@t> rothgen.nhs.uk Wed Mar 19 12:21:27 2008 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Wed Mar 19 12:20:57 2008 Subject: [Histonet] Kidney Bx question Message-ID: <5C0BED61F529364E86309CADEA63FEF20163F3A4@TRFT-EX01.xRothGen.nhs.uk> Do you have a train to catch? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histoinfo@comcast.net Sent: 19 March 2008 17:14 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kidney Bx question We have been having some trouble with the inner surface of the tubules looking torn look to the tissue. We hand process all our kidney bx's with the times as follows. 1-formalin 15 minutes 2-50% ethanol 15 minutes 3-70% ethanol 15 minutes 4-80% ethanol 15 minutes 5-95% ethanol 15 minutes 6-100% ethanol 15 minutes 7-100% ethanol 15 minutes 9-xylene 15 minutes 10-xylene 15 minutes Parraffin x2, 20 minutes each. They are then cut at 1 micron for PAS & JONES and 2 microns for H&E & TRICHROME. We are trying to determine if this tearing is an artifact from processing, collection or some other factor we haven't thought of. Has anyone else come across this or a similar problem. All thoughts and suggestions welcome. Jennifer Saunders HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ###################################################################### This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. ###################################################################### From dcrippen <@t> buckinstitute.org Wed Mar 19 12:23:24 2008 From: dcrippen <@t> buckinstitute.org (Danielle Crippen) Date: Wed Mar 19 12:23:35 2008 Subject: [Histonet] C. Elegans fixation Message-ID: Hello Histo-experts, Can you please share your favorite worm fixation methods with me?? I've had great success in EM prep using 2% PFA 0.2% gluteraldehyde in cocodylate buffer. BUT-I had to "nick" the worms to get infiltration of the fixative. I now have a user who would prefer to avoid the "nicking" technique for his application, but so far, we're experiencing insufficient fixation. Any/all advice is very welcome!!! Many thanks in advance! danielle Danielle Crippen Morphology and Imaging Core From Jason.Burrill <@t> crl.com Wed Mar 19 12:34:46 2008 From: Jason.Burrill <@t> crl.com (Burrill, Jason) Date: Wed Mar 19 12:35:05 2008 Subject: [Histonet] RE: Formalin Spill Containment Protocol Message-ID: <1AD4E907E9B6F648AEF1B3A20A9B0E1ED5B9FB@shr-exch2.na01.crl.com> Michelle, Without going into great detail about what should be required in terms of a cleanup procedure for any chemical spilled you need to look at every situation differently because they all present their own unique situational responses. When I provide training to chemical spill handlers I highlight the importance of looking at the big picture before you dive right in. Take the following example: 500 ml of 10% neutral buffered formalin is spilled on a floor in a room that is 8 ft x 8 ft with normal, 4-12 room changes/hour, air flow. Would you try to clean up that spill without a respirator? I wouldn't and here is why. Because the Short Term Exposure Limit of formaldehyde it 2 ppm for a 15 minute time period you would likely be asking someone to put themselves in a situation where they would be exposed to a level of formaldehyde that will have acute and possibly long term affects. Now here is another scenario: 500 ml of 10% neutral buffered formalin is spilled in a fume hood or grossing hood. Can you clean up that spill with the appropriate PPE (lab coat/apron, splash goggles and gloves? Most likely the answer would be yes because the inhalation hazard that would be presented is negated by the fact that the spilled material is in a ventilated workspace. I know it isn't easy but I have included an excerpt of the workshop I give at Histology meetings as it pertains to chemical spills. Sorry that this is such a long response but your situation is very open ended and requires a lot of thought before implementing a blanket response to all spills. When a Spill Occurs Was anyone exposed? If so immediately administer aid according to the MSDS and seek medical attention if necessary, according to the Formaldehyde and Lab Standard exposed employees must be offered medical attention. When an accident occurs consider identifying someone to assist in aiding the exposed worker. Evaluate MSDS Does the MSDS have specific instructions for cleaning up small or large spills? If so, is there required PPE that you may not have access to (e.g. respirators). Extent of the spill Is this a small spill that can be cleaned up by a non-hazmat trained individual? Which of the following categories would this spill fall into? * Incidental Release - Release of a chemical that if left unattended does not impose an immediate threat to an employee's health and well being. * Manageable Chemical Spill - Release of a hazardous chemical that can be contained with spill agents and PPE on hand, without exposing the employee to unnecessary hazards. * Immediately Dangerous to Life and Health (IDLH) - The concentration of a chemical that is immediately dangerous to life and health. Inhalation of a chemical at this concentration is likely to be lethal or to cause severe, irreversible damage. * Life-Threatening Situations - High concentrations of toxic chemicals, IDLH environments, Oxygen-deficient atmosphere, Fire or explosion hazard or a situation that requires immediate attention because of the danger posed to employees in the area. Evacuate personnel and secure area Instruct personnel to evacuate the lab and if necessary activate the fire pull station. Post personnel or signs in the entryway of the lab to prevent unnecessary exposure to the spill. Contact supervisor/chemical hygiene officer/emergency personnel for cleanup Whomever your institution's protocols say to contact is how you should proceed. If you don't know ask! Jason Burrill Sr. Manager, Histology and Laboratory Safety Charles River Laboratories 251 Ballardvale Street Wilmington, MA 01887 Ph: 781-222-6152 Fax: 978-988-8793 jason.burrill@crl.com **Please note new direct dial telephone number** From Charlotte.Kopczynski <@t> baycare.org Wed Mar 19 12:47:22 2008 From: Charlotte.Kopczynski <@t> baycare.org (Kopczynski, Charlotte) Date: Wed Mar 19 12:47:30 2008 Subject: [Histonet] Histology Position in Florida In-Reply-To: Message-ID: I will have a full-time opening for a Florida licensed Histotech at Morton Plant Hospital in Clearwater, Florida. The hours are 10 pm - 6:30am. We have competitive salaries and shift diff. Thanks, Charlotte Kopczynski, HTL (ASCP) Pathology Manager Morton Plant Mease Health System BayCare Health System 727-461-8246 Confidential: This electronic message and all contents contain information from BayCare Health System which may be privileged, confidential or otherwise protected from disclosure. The information is intended to be for the addressee only. If you are not the addressee, any disclosure, copy, distribution or use of the contents of this message is prohibited. If you have received this electronic message in error, please notify the sender and destroy the original message and all copies. From Heather.D.Renko <@t> osfhealthcare.org Wed Mar 19 12:50:14 2008 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Wed Mar 19 12:50:35 2008 Subject: [Histonet] RE: CPT Code for FNA Message-ID: <40026EDDE64CDA47AB382C52619ACD3C06612DC5@pmc-rfd-mx01.intranet.osfnet.org> We charge CPT 88172 (Cytopathology, evaluations of fine needle aspirate; immediate cytohistologic study to determine adequacy of specimen(s) for the technical component. Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From oshel1pe <@t> cmich.edu Wed Mar 19 13:03:12 2008 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Wed Mar 19 13:03:58 2008 Subject: [Histonet] C. Elegans fixation In-Reply-To: References: Message-ID: Danielle, This is a good question for the microscopy list (subscribe at http://www.microscopy.com ). There's probably a C. elegans list that would be good to consult. There are also excellent resources on the web. A particularly good one is David Hall's lab, the Center for C. elegans anatomy at http://www.aecom.yu.edu/wormem/ There are excellent methods here. Good luck! Intact C. elegans is non-trivial. The cuticle causes much fuss and bother, and most people doing EM use high-pressure freezing methods, followed by freeze-substitution embedding. Phil >Hello Histo-experts, > > > >Can you please share your favorite worm fixation methods with me?? I've >had great success in EM prep using 2% PFA 0.2% gluteraldehyde in >cocodylate buffer. BUT-I had to "nick" the worms to get infiltration of >the fixative. I now have a user who would prefer to avoid the "nicking" >technique for his application, but so far, we're experiencing >insufficient fixation. Any/all advice is very welcome!!! > > > >Many thanks in advance! > > > >danielle > > > >Danielle Crippen > >Morphology and Imaging Core -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576 From mtitford <@t> aol.com Wed Mar 19 13:19:11 2008 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Wed Mar 19 13:19:28 2008 Subject: [Histonet] Train to catch?Yes! Message-ID: <8CA5804DD63B4D0-17C4-1456@WEBMAIL-DF02.sysops.aol.com> Life is so interesting! You can tell where people are coming from by their Histonet emails. In fact, you can see?their professional lives?unfold before you! Jennifer Saunders asked about processing artefacts in renal biopsies. She is using a "short run" schedule so she is probably processing transplant renal biopsies. She probably has a transplant surgeon and a renal pathologist breathing down her neck. They are all wondering why the creatine clearance is high and the patient is not producing urine. Maybe a kidney rejection case. Maybe the patient needs dialysis or another kidney. Then there is Dr Terry Marshall. He is wondering why use a rapid processing schedule. All very interesting! And its not Friday yet! Mike Titford USA Pathology Mobile AL USA From juditw <@t> u.washington.edu Wed Mar 19 13:47:36 2008 From: juditw <@t> u.washington.edu (Judith L. Williams) Date: Wed Mar 19 13:47:41 2008 Subject: [Histonet] OT: Tuesday Tasty Tidbit In-Reply-To: Message-ID: Hey- Your Call!!!! it may be hard to mount in the area I think you mean - but you could try! a weather vane would just indicate the direction you need to move away FROM! Judy On Wed, 19 Mar 2008, Tony Henwood wrote: > I know someone who seems to have lots of wind. > Can I attach one of these "horizontally fixed pitot tubes" to him or would a "vane" do the job? > Seems like a job for a proctologist! > > Or should I just put him in the fume hood! > > I don't know whether I should post this - ah what the heck!! > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) > Laboratory Manager & Senior Scientist > The Children's Hospital at Westmead, > Locked Bag 4001, Westmead, 2145, AUSTRALIA. > Tel: 612 9845 3306 > Fax: 612 9845 3318 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Judith L. Williams > Sent: Wednesday, 19 March 2008 7:41 AM > To: Rene J Buesa > Cc: histonet@lists.utsouthwestern.edu; Breeden, Sara > Subject: Re: [Histonet] OT: Tuesday Tasty Tidbit > > > Tell the person interested in wind speed that they will need a horizontally fixed pitot tube - as in the kind affixed to the wings of airplanes to measure wind speed! that should get them going!!!! you can find out about pitot tubes in Google website- NASA has a good description with diagrams should they want them (haha!) still chuckling over this one, Judy in Washington > > > On Tue, 18 Mar 2008, Rene J Buesa wrote: > >> Sara: >> There is nothing to flame about. It is just a misunderstanding, what >> you HAVE to measure is the wind FLOW in your fume hoods. There are >> small vanes that you place at the entrance, where the air flows the >> most, and one a week (or a day if you wish) you check the vane to >> determine if the air is flowing and at what RATE (or speed if you >> will). I have a vane in each of my 4 fumes hoods, and had a log for >> that determination. Ren? J. >> >> "Breeden, Sara" wrote: >> Okay, so it's not the Friday Hour of Fuming but this will do. >> Without giving away my source, my boss received an email this morning >> asking,"Does anyone monitor wind speed in their histo labs?". My boss >> replied that we don't because our lab is INDOORS. This has been a >> source of great amusement all morning - I know what the person is >> asking, but it just struck me as funny! Adios >> >> >> >> Sally Breeden, HT(ASCP) >> >> NM Dept. of Agriculture >> >> Veterinary Diagnostic Services >> >> PO Box 4700 >> >> Albuquerque, NM 87106 >> >> 505-841-2576 >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> --------------------------------- >> Never miss a thing. Make Yahoo your homepage. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > Judith Williams, PhD, HT(ASCP) > Research Scientist > Department of Comparative Medicine > University of Washington > Seattle, WA 98195 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead > > This note also confirms that this email message has been > virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************** > > Judith Williams, PhD, HT(ASCP) Research Scientist Department of Comparative Medicine University of Washington Seattle, WA 98195 From bwhitaker <@t> brownpathology.com Wed Mar 19 15:02:46 2008 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Wed Mar 19 14:57:46 2008 Subject: [Histonet] Houston, TX-- Histology Lab Manager position available Message-ID: <001501c889fc$337a34f0$9a6e9ed0$@com> Hi All, There is a great histology manager position open at Brown & Associates Medical Laboratories in Houston, Texas, just outside of the Texas Medical Center. I have held the position since the lab opened 4 years ago, but I am relocating out-of-state for family reasons. It really is a great position! Brown & Associates is a wonderful place to work, with good benefits, a GREAT retirement program, a generous salary and outstanding people to work with. (There is also free parking, right outside of the door!) It is the CAP inspected, private laboratory for a well-established group of 16 pathologists. The lab does the private, surgery center work. The workload is probably going to be about 14,000 surgical specimens this year (primarily GI biopsies), the standard special stains, done manually, and with no gyn cytology (just an occasional esophageal brushing or FNA). The ideal candidate for the manager position will be classified as "high-complexity testing personnel" under CLIA, and will have grossing experience, will have at least 10 years experience in histology with an HT or HTL, and 3-5 years experience as supervisor. A strong background in IT would be a plus, as would experience supervising, or involvement in, the transcription area. I will be available as contact person regarding this position until April 25. After that, contact Joanne McDonald (jmcdonald@brownpathology.com) at the same phone number. Bonnie P. Whitaker Laboratory Manager Brown & Associates Medical Laboratories 8076 El Rio Houston, TX 77054 713.741.6677 FAX 713.748.5860 www.brownpathology.com bwhitaker@brownpathology.com From JMitchell <@t> uwhealth.org Wed Mar 19 15:12:47 2008 From: JMitchell <@t> uwhealth.org (Mitchell Jean A.) Date: Wed Mar 19 15:12:52 2008 Subject: [Histonet] 2008 Tri-State Symposium Message-ID: <713A63AAF4280941A2EF88D66838F9A002612C4F@uwhis-xchng4.uwhis.hosp.wisc.edu> You are invited to roll out the red carpet with the histology societies of Minnesota, Wisconsin and Iowa as they host "Lights, Camera, Action, Histology" the 2008 Tri-State Symposium, April 23-25, at Hotel Sofitel, Bloomington, Minnesota. All inclusive registration fee of $100 (+ state membership) covers workshops, seminars, lunches and our infamous costume banquet. The 2008 banquet theme will be heading to Hollywood and "The Famous or Fake Celebrity Show". CEU Approved Workshops & Seminars Offered: Thursday April 24th: Eyes on Exposure, CISH Her2: An Alternative to IHC & FISH?, A Family Affair: A Systems Approach to Team Development, Preparing for a Mass Disaster: Are You Ready?, Where We're Been, Where We Are and Where We Are Going With Tissue Processing. Friday April 25th: Top 10 Misconceptions About Crime Scene Investigation, What Means These Bones: Death Investigations Involving Skeletonized Human Remains, Entomology & Death: Solving Mysteries With Maggots, Quantum Dots In Clinical & Research Settings, Serial Killers, Forensics & Histotechnology, How Can I Find It? And What Do I Do Once I Have It?, Quality Control of Rodent Tissue in the Research Laboratory. For further information contact any of the states representatives: Minnesota: Colleen Forster (cforster@umn.edu) or Lois Rowe (rowe.lois@mayo.edu) Wisconsin: Maureen Decorah (decorah@rarc.wisc.edu) or Jean Mitchell (jmitchell@uwhealth.org) Iowa: Judi Stasko (judith.stasko@ars.usda.gov) or Dave Cavanaugh (dcavan55@yahoo.com) From Pat.Bell <@t> UCHSC.edu Wed Mar 19 17:15:04 2008 From: Pat.Bell <@t> UCHSC.edu (Pat.Bell@UCHSC.edu) Date: Wed Mar 19 17:15:08 2008 Subject: [Histonet] CD68 antibody for mouse Message-ID: <71FCC52823941D49A4BC39B48C6E3428F3BF4D@java.uchsc.edu> Hello to all I am looking for CD68 in the mouse. Does anyone know of an antibody that will work in FFPE sections? I want to do DAB, not fluorescence. Thank you, Pat Bell HT(ASCP) Sr. PRA Division of Medical Oncology UCHSC, Aurora, CO 303-724-3845 (lab) 303-724-3889 (fax) From san.htin <@t> yahoo.com Wed Mar 19 23:35:13 2008 From: san.htin <@t> yahoo.com (San Tin) Date: Wed Mar 19 23:35:17 2008 Subject: [Histonet] ISH on Bondmax Message-ID: <602251.81143.qm@web55814.mail.re3.yahoo.com> Hi All, Any one out there have ever used ISH probes other than Bond and Novocastra on BondMax( leica-microsyste)? Sincerely, San ____________________________________________________________________________________ Looking for last minute shopping deals? Find them fast with Yahoo! Search. http://tools.search.yahoo.com/newsearch/category.php?category=shopping From kemlo <@t> f2s.com Thu Mar 20 03:27:38 2008 From: kemlo <@t> f2s.com (kemlo) Date: Thu Mar 20 03:29:06 2008 Subject: [Histonet] Kidney Bx question In-Reply-To: <5C0BED61F529364E86309CADEA63FEF20163F3A4@TRFT-EX01.xRothGen.nhs.uk> References: <5C0BED61F529364E86309CADEA63FEF20163F3A4@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <886B0C4C8E5B46B49BE0D8B18823F46A@KemloPC> Process in haste, repent at leisure................. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: 19 March 2008 17:21 To: histoinfo@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Kidney Bx question Do you have a train to catch? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histoinfo@comcast.net Sent: 19 March 2008 17:14 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kidney Bx question We have been having some trouble with the inner surface of the tubules looking torn look to the tissue. We hand process all our kidney bx's with the times as follows. 1-formalin 15 minutes 2-50% ethanol 15 minutes 3-70% ethanol 15 minutes 4-80% ethanol 15 minutes 5-95% ethanol 15 minutes 6-100% ethanol 15 minutes 7-100% ethanol 15 minutes 9-xylene 15 minutes 10-xylene 15 minutes Parraffin x2, 20 minutes each. They are then cut at 1 micron for PAS & JONES and 2 microns for H&E & TRICHROME. We are trying to determine if this tearing is an artifact from processing, collection or some other factor we haven't thought of. Has anyone else come across this or a similar problem. All thoughts and suggestions welcome. Jennifer Saunders HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ###################################################################### This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. ###################################################################### _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo <@t> f2s.com Thu Mar 20 03:30:36 2008 From: kemlo <@t> f2s.com (kemlo) Date: Thu Mar 20 03:32:02 2008 Subject: [Histonet] Train to catch?Yes! In-Reply-To: <8CA5804DD63B4D0-17C4-1456@WEBMAIL-DF02.sysops.aol.com> References: <8CA5804DD63B4D0-17C4-1456@WEBMAIL-DF02.sysops.aol.com> Message-ID: <5D9235648A234D3A825CB0997144F9E8@KemloPC> Oh so renal biopsy stat!!! Is life really that hectic? Maybe a little more time spent gets the correct result rather than rushing and ruining. Tortoise and the hare? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mtitford@aol.com Sent: 19 March 2008 18:19 To: Histonet@pathology.swmed.edu Subject: [Histonet] Train to catch?Yes! Life is so interesting! You can tell where people are coming from by their Histonet emails. In fact, you can see?their professional lives?unfold before you! Jennifer Saunders asked about processing artefacts in renal biopsies. She is using a "short run" schedule so she is probably processing transplant renal biopsies. She probably has a transplant surgeon and a renal pathologist breathing down her neck. They are all wondering why the creatine clearance is high and the patient is not producing urine. Maybe a kidney rejection case. Maybe the patient needs dialysis or another kidney. Then there is Dr Terry Marshall. He is wondering why use a rapid processing schedule. All very interesting! And its not Friday yet! Mike Titford USA Pathology Mobile AL USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Thu Mar 20 06:12:18 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Mar 20 06:08:56 2008 Subject: [Histonet] air cleaning Message-ID: <1F937FB30BDB7C4A9F39F83FEA8D379F9F532C@bruexchange1.digestivespecialists.com> Does anyone have any experience or know of something called UV air cleaning for fumes in the histo lab? Thanks, Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com From mohs76009 <@t> yahoo.com Thu Mar 20 07:04:00 2008 From: mohs76009 <@t> yahoo.com (Matt Bancroft) Date: Thu Mar 20 07:04:07 2008 Subject: [Histonet] CLIA In-Reply-To: <1F937FB30BDB7C4A9F39F83FEA8D379F9F532C@bruexchange1.digestivespecialists.com> Message-ID: <320362.54247.qm@web63401.mail.re1.yahoo.com> For those of you that have to go thru a CLIA inspeciton, I had mine yesterday and came across a issue, that in my 15 years of histology have never came across so I thought that I would share with you all. The inspector that I had opened up the owners manuals of my equipment (paraffin pot, water-bath, embedding center, etc) and looked at the manufactors recommendations for room temp and room humidity. She was going to ding me on not haveing those ranges on my QC chats, but I changed them while she was here. So if you do not have those ranges on your QC charts I would recommened it. Matt Bancroft Laboratory Manager --------------------------------- Never miss a thing. Make Yahoo your homepage. From histoinfo <@t> comcast.net Thu Mar 20 07:10:15 2008 From: histoinfo <@t> comcast.net (histoinfo@comcast.net) Date: Thu Mar 20 07:10:31 2008 Subject: [Histonet] Train to catch?Yes! Message-ID: <032020081210.27739.47E25427000E910400006C5B221655140601000207019B9C0708@comcast.net> I guess I am surprised at all the comments about this being such a rush processing schedule. These are very small needle core bxs, and the processing takes about 3 hours to complete. Is anyone else using hand processing willing to share their protocol please? Jennifer -------------- Original message -------------- From: "kemlo" > Oh so renal biopsy stat!!! > > Is life really that hectic? Maybe a little more time spent gets the correct > result rather than rushing and ruining. > > Tortoise and the hare? > From rjbuesa <@t> yahoo.com Thu Mar 20 07:18:07 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 20 07:18:17 2008 Subject: [Histonet] Kidney Bx question In-Reply-To: <5C0BED61F529364E86309CADEA63FEF20163F3A4@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <6128.45084.qm@web65713.mail.ac4.yahoo.com> For hand processing at room temperature that schedule is too short, unless the samples are agitated. Ren? J. "Marshall Terry Dr, Consultant Histopathologist" wrote: Do you have a train to catch? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histoinfo@comcast.net Sent: 19 March 2008 17:14 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kidney Bx question We have been having some trouble with the inner surface of the tubules looking torn look to the tissue. We hand process all our kidney bx's with the times as follows. 1-formalin 15 minutes 2-50% ethanol 15 minutes 3-70% ethanol 15 minutes 4-80% ethanol 15 minutes 5-95% ethanol 15 minutes 6-100% ethanol 15 minutes 7-100% ethanol 15 minutes 9-xylene 15 minutes 10-xylene 15 minutes Parraffin x2, 20 minutes each. They are then cut at 1 micron for PAS & JONES and 2 microns for H&E & TRICHROME. We are trying to determine if this tearing is an artifact from processing, collection or some other factor we haven't thought of. Has anyone else come across this or a similar problem. All thoughts and suggestions welcome. Jennifer Saunders HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ###################################################################### This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. ###################################################################### _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From histoinfo <@t> comcast.net Thu Mar 20 08:01:13 2008 From: histoinfo <@t> comcast.net (histoinfo@comcast.net) Date: Thu Mar 20 08:01:24 2008 Subject: [Histonet] Kidney Bx question Message-ID: <032020081301.8406.47E26019000C9252000020D6221557867401000207019B9C0708@comcast.net> Rene, Yes they are agitated. And the tearing problem that I originally described is fairly infrequent. It's just that when it does happen we can't figure out why. When it does happen nothing that we can see is any different than any other day. Nothing in common as far as collection site either. I am beginning to think more and more that it may be on days that we have 5 or 6 bxs that are all run together. But even then it is not every bx. I think we will take better steps to prevent solution carryover and see what happens. Of course it is so infrequent that we have this problem that it will be months before we can say that we "fixed" the problem. I would still like to compare times with others out there though. So please share your times for hand processing. Thanks, Jennifer -------------- Original message -------------- From: Rene J Buesa For hand processing at room temperature that schedule is too short, unless the samples are agitated. René J. "Marshall Terry Dr, Consultant Histopathologist" wrote: Do you have a train to catch? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histoinfo@comcast.net Sent: 19 March 2008 17:14 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kidney Bx question We have been having some trouble with the inner surface of the tubules looking torn look to the tissue. We hand process all our kidney bx's with the times as follows. 1-formalin 15 minutes 2-50% ethanol 15 minutes 3-70% ethanol 15 minutes 4-80% ethanol 15 minutes 5-95% ethanol 15 minutes 6-100% ethanol 15 minutes 7-100% ethanol 15 minutes 9-xylene 15 minutes 10-xylene 15 minutes Parraffin x2, 20 minutes each. They are then cut at 1 micron for PAS & JONES and 2 microns for H&E & TRICHROME. We are trying to determine if this tearing is an artifact from processing, collection or some other factor we haven't thought of. Has anyone else come across this or a similar problem. All thoughts and suggestions welcome. Jennifer Saunders HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ###################################################################### This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. ###################################################################### _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From gu.lang <@t> gmx.at Thu Mar 20 08:53:02 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Mar 20 08:53:18 2008 Subject: AW: [Histonet] Kidney Bx question In-Reply-To: <032020081301.8406.47E26019000C9252000020D6221557867401000207019B9C0708@comcast.net> Message-ID: <000601c88a91$b95251d0$eeeea8c0@dielangs.at> Would you be so kind and explain, why you have to do a short run. Are these indeed NTX-biopsies, that need a report as fast as possible? Or is it just the fear to over-process the biopsies? We have ntx from time to time, but even then, the clinicians wait for the report until the next day, noon. So we can process the biopsies with our regular histo and do the specialstains (Jones, PAS, Congored, EvG, SFOG = kind of trichrome), immunohistochemistry and immunofluorescence. We process the needlebiopsies in sponges and with the regular 13-hour protocol. Regarding IHC a fixation-time under 6 hours could also drive to problems. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von histoinfo@comcast.net Gesendet: Donnerstag, 20. M?rz 2008 14:01 An: Rene J Buesa; Marshall Terry Dr,Consultant Histopathologist; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] Kidney Bx question Rene, Yes they are agitated. And the tearing problem that I originally described is fairly infrequent. It's just that when it does happen we can't figure out why. When it does happen nothing that we can see is any different than any other day. Nothing in common as far as collection site either. I am beginning to think more and more that it may be on days that we have 5 or 6 bxs that are all run together. But even then it is not every bx. I think we will take better steps to prevent solution carryover and see what happens. Of course it is so infrequent that we have this problem that it will be months before we can say that we "fixed" the problem. I would still like to compare times with others out there though. So please share your times for hand processing. Thanks, Jennifer -------------- Original message -------------- From: Rene J Buesa For hand processing at room temperature that schedule is too short, unless the samples are agitated. Ren? J. "Marshall Terry Dr, Consultant Histopathologist" wrote: Do you have a train to catch? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histoinfo@comcast.net Sent: 19 March 2008 17:14 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kidney Bx question We have been having some trouble with the inner surface of the tubules looking torn look to the tissue. We hand process all our kidney bx's with the times as follows. 1-formalin 15 minutes 2-50% ethanol 15 minutes 3-70% ethanol 15 minutes 4-80% ethanol 15 minutes 5-95% ethanol 15 minutes 6-100% ethanol 15 minutes 7-100% ethanol 15 minutes 9-xylene 15 minutes 10-xylene 15 minutes Parraffin x2, 20 minutes each. They are then cut at 1 micron for PAS & JONES and 2 microns for H&E & TRICHROME. We are trying to determine if this tearing is an artifact from processing, collection or some other factor we haven't thought of. Has anyone else come across this or a similar problem. All thoughts and suggestions welcome. Jennifer Saunders HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ###################################################################### This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. ###################################################################### _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RRA <@t> Stowers-Institute.org Thu Mar 20 09:06:07 2008 From: RRA <@t> Stowers-Institute.org (Allen, Rhonda) Date: Thu Mar 20 09:06:56 2008 Subject: [Histonet] c. elegans In-Reply-To: References: Message-ID: The only way I know to fix them without nicking them is to use a high pressure freezer and then use freeze substitution. Thanks, Rhonda ------------------------------ Message: 4 Date: Wed, 19 Mar 2008 10:23:24 -0700 From: "Danielle Crippen" Subject: [Histonet] C. Elegans fixation To: Cc: Cathy Vitelli Message-ID: Content-Type: text/plain; charset="us-ascii" Hello Histo-experts, Can you please share your favorite worm fixation methods with me?? I've had great success in EM prep using 2% PFA 0.2% gluteraldehyde in cocodylate buffer. BUT-I had to "nick" the worms to get infiltration of the fixative. I now have a user who would prefer to avoid the "nicking" technique for his application, but so far, we're experiencing insufficient fixation. Any/all advice is very welcome!!! Many thanks in advance! danielle Danielle Crippen Morphology and Imaging Core From mickie25 <@t> netzero.net Thu Mar 20 09:07:27 2008 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Thu Mar 20 09:07:22 2008 Subject: [Histonet] CLIA In-Reply-To: <320362.54247.qm@web63401.mail.re1.yahoo.com> References: <1F937FB30BDB7C4A9F39F83FEA8D379F9F532C@bruexchange1.digestivespecialists.com> <320362.54247.qm@web63401.mail.re1.yahoo.com> Message-ID: Matt, I think this is interesting because I am hearing more and more that CLIA inspectors are requiring these ranges on the chart. Is anyone else having CLIA inspectors ding them about operational humidity and temperature ranges on temperature charts? Some CLIA inspectors require room temperatures to be recorded as well. Everyone's input would be appreciated. Thanks. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matt Bancroft Sent: Thursday, March 20, 2008 5:04 AM To: Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: [Histonet] CLIA For those of you that have to go thru a CLIA inspeciton, I had mine yesterday and came across a issue, that in my 15 years of histology have never came across so I thought that I would share with you all. The inspector that I had opened up the owners manuals of my equipment (paraffin pot, water-bath, embedding center, etc) and looked at the manufactors recommendations for room temp and room humidity. She was going to ding me on not haveing those ranges on my QC chats, but I changed them while she was here. So if you do not have those ranges on your QC charts I would recommened it. Matt Bancroft Laboratory Manager --------------------------------- Never miss a thing. Make Yahoo your homepage. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Gguerzon <@t> lifebridgehealth.org Thu Mar 20 09:16:45 2008 From: Gguerzon <@t> lifebridgehealth.org (Godfrey Guerzon) Date: Thu Mar 20 09:17:20 2008 Subject: AW: [Histonet] Kidney Bx question Message-ID: We are supposed to be professionals. What we post are real issues in our situation and ask for help. Professionals don't make snide remarks and put down our colleagues. If we think that it will raise our stature as professionals when we make a joke and condescending comments on things that we need help on, we are greatly mistaken. A wise man once said "if you have nothing good to say about anything - shut up". We need to understand that each situation is different - who are we to judge? We post a real issue in our own situation and we don't need "smart" colleagues make a joke of our situation. Do we want to be treated as professionals - then let us communicate as professionals. No snide remarks or condescending comments - PLEASE!!! - Help and good ideas are welcome - if you have nothiing to contribute to the issue at hand - please don't waste your (and ours) valuable time with useless comments. Godfrey >>> "Gudrun Lang" 3/20/2008 9:53 AM >>> Would you be so kind and explain, why you have to do a short run. Are these indeed NTX-biopsies, that need a report as fast as possible? Or is it just the fear to over-process the biopsies? We have ntx from time to time, but even then, the clinicians wait for the report until the next day, noon. So we can process the biopsies with our regular histo and do the specialstains (Jones, PAS, Congored, EvG, SFOG = kind of trichrome), immunohistochemistry and immunofluorescence. We process the needlebiopsies in sponges and with the regular 13-hour protocol. Regarding IHC a fixation-time under 6 hours could also drive to problems. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von histoinfo@comcast.net Gesendet: Donnerstag, 20. M?rz 2008 14:01 An: Rene J Buesa; Marshall Terry Dr,Consultant Histopathologist; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] Kidney Bx question Rene, Yes they are agitated. And the tearing problem that I originally described is fairly infrequent. It's just that when it does happen we can't figure out why. When it does happen nothing that we can see is any different than any other day. Nothing in common as far as collection site either. I am beginning to think more and more that it may be on days that we have 5 or 6 bxs that are all run together. But even then it is not every bx. I think we will take better steps to prevent solution carryover and see what happens. Of course it is so infrequent that we have this problem that it will be months before we can say that we "fixed" the problem. I would still like to compare times with others out there though. So please share your times for hand processing. Thanks, Jennifer -------------- Original message -------------- From: Rene J Buesa For hand processing at room temperature that schedule is too short, unless the samples are agitated. Ren? J. "Marshall Terry Dr, Consultant Histopathologist" wrote: Do you have a train to catch? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histoinfo@comcast.net Sent: 19 March 2008 17:14 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kidney Bx question We have been having some trouble with the inner surface of the tubules looking torn look to the tissue. We hand process all our kidney bx's with the times as follows. 1-formalin 15 minutes 2-50% ethanol 15 minutes 3-70% ethanol 15 minutes 4-80% ethanol 15 minutes 5-95% ethanol 15 minutes 6-100% ethanol 15 minutes 7-100% ethanol 15 minutes 9-xylene 15 minutes 10-xylene 15 minutes Parraffin x2, 20 minutes each. They are then cut at 1 micron for PAS & JONES and 2 microns for H&E & TRICHROME. We are trying to determine if this tearing is an artifact from processing, collection or some other factor we haven't thought of. Has anyone else come across this or a similar problem. All thoughts and suggestions welcome. Jennifer Saunders HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ###################################################################### This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. ###################################################################### _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------------------------------- THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE ADDRESSEE LISTED ABOVE. This record has been disclosed in accordance with Subtitle 3 of Title 4 of the Health-General Article of the Annotated Code of Maryland. Further disclosure of medical information contained herein is prohibited. If you are neither the intended recipient nor the individual responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure of patient information is strictly prohibited. If you have received this email in error, immediately notify us by telephone or return email. ----------------------------------------------------------------- From mward <@t> wfubmc.edu Thu Mar 20 09:26:10 2008 From: mward <@t> wfubmc.edu (Martha Ward) Date: Thu Mar 20 09:26:14 2008 Subject: [Histonet] Inhibin antibody on the Bond Message-ID: <61135F0455D33347B5AAE209B903A30421D18AD7@EXCHVS2.medctr.ad.wfubmc.edu> I am trying to work up Inhibin on the Bond and I am having a bit of trouble. I currently use one from CellMarque but I am not happy with the results. What vendor/antibody are others using? Thanks in advance for your help! Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center Medical Center Blvd. Winston-Salem, NC 27157 336-716-2756 mward@wfubmc.edu From kemlo <@t> f2s.com Thu Mar 20 09:27:53 2008 From: kemlo <@t> f2s.com (kemlo) Date: Thu Mar 20 09:29:24 2008 Subject: [Histonet] Kidney Bx question In-Reply-To: <6128.45084.qm@web65713.mail.ac4.yahoo.com> References: <5C0BED61F529364E86309CADEA63FEF20163F3A4@TRFT-EX01.xRothGen.nhs.uk> <6128.45084.qm@web65713.mail.ac4.yahoo.com> Message-ID: I'd get agitated if I was processing unfixed tissue (15 min?) so quickly . -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 20 March 2008 12:18 To: Marshall Terry Dr,Consultant Histopathologist; histoinfo@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Kidney Bx question For hand processing at room temperature that schedule is too short, unless the samples are agitated. Ren? J. "Marshall Terry Dr, Consultant Histopathologist" wrote: Do you have a train to catch? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histoinfo@comcast.net Sent: 19 March 2008 17:14 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kidney Bx question We have been having some trouble with the inner surface of the tubules looking torn look to the tissue. We hand process all our kidney bx's with the times as follows. 1-formalin 15 minutes 2-50% ethanol 15 minutes 3-70% ethanol 15 minutes 4-80% ethanol 15 minutes 5-95% ethanol 15 minutes 6-100% ethanol 15 minutes 7-100% ethanol 15 minutes 9-xylene 15 minutes 10-xylene 15 minutes Parraffin x2, 20 minutes each. They are then cut at 1 micron for PAS & JONES and 2 microns for H&E & TRICHROME. We are trying to determine if this tearing is an artifact from processing, collection or some other factor we haven't thought of. Has anyone else come across this or a similar problem. All thoughts and suggestions welcome. Jennifer Saunders HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ###################################################################### This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. ###################################################################### _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo <@t> f2s.com Thu Mar 20 09:29:26 2008 From: kemlo <@t> f2s.com (kemlo) Date: Thu Mar 20 09:31:01 2008 Subject: [Histonet] Train to catch?Yes! In-Reply-To: <032020081210.27739.47E25427000E910400006C5B221655140601000207019B9C0708@comcast.net> References: <032020081210.27739.47E25427000E910400006C5B221655140601000207019B9C0708@comcast.net> Message-ID: <257EFA089A03407B898BA8E9BB85FAAC@KemloPC> 15 mins will not fix anything!!! That's your major issue Take care _____ From: histoinfo@comcast.net [mailto:histoinfo@comcast.net] Sent: 20 March 2008 12:10 To: kemlo; mtitford@aol.com; Histonet@pathology.swmed.edu Subject: RE: [Histonet] Train to catch?Yes! I guess I am surprised at all the comments about this being such a rush processing schedule. These are very small needle core bxs, and the processing takes about 3 hours to complete. Is anyone else using hand processing willing to share their protocol please? Jennifer -------------- Original message -------------- From: "kemlo" > Oh so renal biopsy stat!!! > > Is life really that hectic? Maybe a little more time spent gets the correct > result rather than rushing and ruining. > > Tortoise and the hare? > From Terry.Marshall <@t> rothgen.nhs.uk Thu Mar 20 09:31:31 2008 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Thu Mar 20 09:31:08 2008 Subject: AW: [Histonet] Kidney Bx question Message-ID: <5C0BED61F529364E86309CADEA63FEF20163F3A5@TRFT-EX01.xRothGen.nhs.uk> Godfrey, Nobody has said anything snide, or put anybody down. Your post is typical of those lacking a sense of humour, and who think being professional is synonymous with being formal. It is not. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Godfrey Guerzon Sent: 20 March 2008 14:17 To: histoinfo@comcast.net; Gudrun Lang Cc: histonet@lists.utsouthwestern.edu Subject: Re: AW: [Histonet] Kidney Bx question We are supposed to be professionals. What we post are real issues in our situation and ask for help. Professionals don't make snide remarks and put down our colleagues. If we think that it will raise our stature as professionals when we make a joke and condescending comments on things that we need help on, we are greatly mistaken. A wise man once said "if you have nothing good to say about anything - shut up". We need to understand that each situation is different - who are we to judge? We post a real issue in our own situation and we don't need "smart" colleagues make a joke of our situation. Do we want to be treated as professionals - then let us communicate as professionals. No snide remarks or condescending comments - PLEASE!!! - Help and good ideas are welcome - if you have nothiing to contribute to the issue at hand - please don't waste your (and ours) valuable time with useless comments. Godfrey >>> "Gudrun Lang" 3/20/2008 9:53 AM >>> Would you be so kind and explain, why you have to do a short run. Are these indeed NTX-biopsies, that need a report as fast as possible? Or is it just the fear to over-process the biopsies? We have ntx from time to time, but even then, the clinicians wait for the report until the next day, noon. So we can process the biopsies with our regular histo and do the specialstains (Jones, PAS, Congored, EvG, SFOG = kind of trichrome), immunohistochemistry and immunofluorescence. We process the needlebiopsies in sponges and with the regular 13-hour protocol. Regarding IHC a fixation-time under 6 hours could also drive to problems. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von histoinfo@comcast.net Gesendet: Donnerstag, 20. M?rz 2008 14:01 An: Rene J Buesa; Marshall Terry Dr,Consultant Histopathologist; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] Kidney Bx question Rene, Yes they are agitated. And the tearing problem that I originally described is fairly infrequent. It's just that when it does happen we can't figure out why. When it does happen nothing that we can see is any different than any other day. Nothing in common as far as collection site either. I am beginning to think more and more that it may be on days that we have 5 or 6 bxs that are all run together. But even then it is not every bx. I think we will take better steps to prevent solution carryover and see what happens. Of course it is so infrequent that we have this problem that it will be months before we can say that we "fixed" the problem. I would still like to compare times with others out there though. So please share your times for hand processing. Thanks, Jennifer -------------- Original message -------------- From: Rene J Buesa For hand processing at room temperature that schedule is too short, unless the samples are agitated. Ren? J. "Marshall Terry Dr, Consultant Histopathologist" wrote: Do you have a train to catch? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histoinfo@comcast.net Sent: 19 March 2008 17:14 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kidney Bx question We have been having some trouble with the inner surface of the tubules looking torn look to the tissue. We hand process all our kidney bx's with the times as follows. 1-formalin 15 minutes 2-50% ethanol 15 minutes 3-70% ethanol 15 minutes 4-80% ethanol 15 minutes 5-95% ethanol 15 minutes 6-100% ethanol 15 minutes 7-100% ethanol 15 minutes 9-xylene 15 minutes 10-xylene 15 minutes Parraffin x2, 20 minutes each. They are then cut at 1 micron for PAS & JONES and 2 microns for H&E & TRICHROME. We are trying to determine if this tearing is an artifact from processing, collection or some other factor we haven't thought of. Has anyone else come across this or a similar problem. All thoughts and suggestions welcome. Jennifer Saunders HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ###################################################################### This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. ###################################################################### _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------------------------------- THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE ADDRESSEE LISTED ABOVE. This record has been disclosed in accordance with Subtitle 3 of Title 4 of the Health-General Article of the Annotated Code of Maryland. Further disclosure of medical information contained herein is prohibited. If you are neither the intended recipient nor the individual responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure of patient information is strictly prohibited. If you have received this email in error, immediately notify us by telephone or return email. ----------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Thu Mar 20 09:39:47 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Thu Mar 20 09:39:59 2008 Subject: [Histonet] Train to catch?Yes! In-Reply-To: <257EFA089A03407B898BA8E9BB85FAAC@KemloPC> References: <032020081210.27739.47E25427000E910400006C5B221655140601000207019B9C0708@comcast.net> <257EFA089A03407B898BA8E9BB85FAAC@KemloPC> Message-ID: <47E27733.70306@pathology.washington.edu> I don't think it has been established that the tissue is unfixed. Our facility has a large renal service and our processing times are even shorter, but we use an automated processor. Most of our cases are sent to us so the tissue is fixed. Victor kemlo wrote: > 15 mins will not fix anything!!! That's your major issue > > Take care > > > > _____ > > From: histoinfo@comcast.net [mailto:histoinfo@comcast.net] > Sent: 20 March 2008 12:10 > To: kemlo; mtitford@aol.com; Histonet@pathology.swmed.edu > Subject: RE: [Histonet] Train to catch?Yes! > > > > I guess I am surprised at all the comments about this being such a rush > processing schedule. These are very small needle core bxs, and the > processing takes about 3 hours to complete. Is anyone else using hand > processing willing to share their protocol please? > > Jennifer > > > > -------------- Original message -------------- > From: "kemlo" > > >> Oh so renal biopsy stat!!! >> >> Is life really that hectic? Maybe a little more time spent gets the >> > correct > >> result rather than rushing and ruining. >> >> Tortoise and the hare? >> >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From mpkrause <@t> yorku.ca Thu Mar 20 09:40:22 2008 From: mpkrause <@t> yorku.ca (mpkrause@yorku.ca) Date: Thu Mar 20 09:42:33 2008 Subject: [Histonet] IF sections look rumpled, in/out of focus Message-ID: <1206024022.47e277565bce5@mymail.yorku.ca> Hi, I'm not sure if I can get a question posted this way but here's a try: When I do immunofluorescence for adiponectin in mouse skeletal muscle cross sections, the section ends up looking very warped and rumpled and is in and out of focus, almost like it was trying to lift/wash right off the slide. I've tried several fixations (4% PFA, Acetone, no fix at all), I've tried putting them in a 60 C oven for an hour, I've tried air drying, and it's always the same result. I tried taking sections and just soaking them in several changes of PBS to simulate doing the incubation steps in our IF protocol and found the sections were doing the same thing. I use gold plus slides, sections are 10um thick (i'd go thinner but our cryostat does not give consistent thickness below 10um), and come from frozen unfixed muscle. I've also tried PBS with extra Ca and Mg, but no luck. thanks Matt From ploykasek <@t> phenopath.com Thu Mar 20 09:43:07 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Thu Mar 20 09:43:21 2008 Subject: [Histonet] Inhibin antibody on the Bond In-Reply-To: <61135F0455D33347B5AAE209B903A30421D18AD7@EXCHVS2.medctr.ad.wfubmc.edu> Message-ID: I do not run Inhibin on the Bond, but do use it in IHC. We use mouse monoclonal clone R1 from Serotec. We use heat antigen retrieval and a polymer detection. Just a note, I do find it most helpful when people reference exactly what clone of antibody they are using, name & Manufacturer only give part of the info. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > I am trying to work up Inhibin on the Bond and I am having a bit of > trouble. I currently use one from CellMarque but I am not happy with > the results. What vendor/antibody are others using? Thanks in > advance for your help! > > Martha Ward, MT (ASCP) QIHC > Assistant Manager, Molecular Diagnostics Lab > Wake Forest University Baptist Medical Center > Medical Center Blvd. > Winston-Salem, NC 27157 > 336-716-2756 > mward@wfubmc.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From Ronald.Houston <@t> nationwidechildrens.org Thu Mar 20 09:50:28 2008 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Thu Mar 20 09:50:57 2008 Subject: [Histonet] Inhibin antibody on the Bond In-Reply-To: <61135F0455D33347B5AAE209B903A30421D18AD7@EXCHVS2.medctr.ad.wfubmc.edu> Message-ID: <979FF5962E234F45B06CF0DB7C1AABB21617F3BC@chi2k3ms01.columbuschildrens.net> Great results with Dako's antibody, #M3609. dilution 1:300, ER2, no enhancement Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 Ronald.Houston@NationwideChildrens.org Columbus Children's Hospital is now Nationwide Children's Hospital www.NationwideChildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward Sent: Thursday, March 20, 2008 10:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Inhibin antibody on the Bond I am trying to work up Inhibin on the Bond and I am having a bit of trouble. I currently use one from CellMarque but I am not happy with the results. What vendor/antibody are others using? Thanks in advance for your help! Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center Medical Center Blvd. Winston-Salem, NC 27157 336-716-2756 mward@wfubmc.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From histoinfo <@t> comcast.net Thu Mar 20 09:51:07 2008 From: histoinfo <@t> comcast.net (histoinfo@comcast.net) Date: Thu Mar 20 09:51:20 2008 Subject: [Histonet] Train to catch?Yes! Message-ID: <032020081451.7799.47E279DB00073E3200001E77221655140601000207019B9C0708@comcast.net> The 15 minute formalin station is only used for same dayers. And mostly just for a little extra time in formalin with agitation if we don't know how long it has already been in formalin. Often when tissue comes to us fresh we will give it at least a half an hour. But remember these are very small needle cores, we are talking smaller than your average prostate bx. We don't process them on our bx VIP at the request of our pathologist due to compression of the tissue. This happens even if we turn off the vacuum. I have not been offended by anyones comments at all, rather enjoyed them to be honest. It is the comments by everyone that gives me the insight to look at what we do and how we do it with a different point of view. This is exactly what I am looking for when I post a question to the histonet. You are all wonderful and a great asset when trying to figure something out or looking for a new protocol. Thank You, Thank You, Jennifer -------------- Original message -------------- From: Victor Tobias > I don't think it has been established that the tissue is unfixed. Our > facility has a large renal service and our processing times are even > shorter, but we use an automated processor. Most of our cases are sent > to us so the tissue is fixed. > > Victor > > kemlo wrote: > > 15 mins will not fix anything!!! That's your major issue > > > > Take care > > > > > > > > _____ > > > > From: histoinfo@comcast.net [mailto:histoinfo@comcast.net] > > Sent: 20 March 2008 12:10 > > To: kemlo; mtitford@aol.com; Histonet@pathology.swmed.edu > > Subject: RE: [Histonet] Train to catch?Yes! > > > > > > > > I guess I am surprised at all the comments about this being such a rush > > processing schedule. These are very small needle core bxs, and the > > processing takes about 3 hours to complete. Is anyone else using hand > > processing willing to share their protocol please? > > > > Jennifer > > > > > > > > -------------- Original message -------------- > > From: "kemlo" > > > > > >> Oh so renal biopsy stat!!! > >> > >> Is life really that hectic? Maybe a little more time spent gets the > >> > > correct > > > >> result rather than rushing and ruining. > >> > >> Tortoise and the hare? > >> > >> > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- > Victor Tobias > Clinical Applications Analyst > University of Washington Medical Center > Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > victor@pathology.washington.edu > 206-598-2792 > 206-598-7659 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments. > From lblazek <@t> digestivespecialists.com Thu Mar 20 09:57:24 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Mar 20 09:54:06 2008 Subject: [Histonet] CLIA In-Reply-To: References: <1F937FB30BDB7C4A9F39F83FEA8D379F9F532C@bruexchange1.digestivespecialists.com> <320362.54247.qm@web63401.mail.re1.yahoo.com> Message-ID: <1F937FB30BDB7C4A9F39F83FEA8D379F9F5332@bruexchange1.digestivespecialists.com> The last inspection I had the inspector told me it might be a good idea to document the humidity levels. I was already documenting the room temp. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: Mickie Johnson [mailto:mickie25@netzero.net] Sent: Thursday, March 20, 2008 10:07 AM To: 'Matt Bancroft'; Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CLIA Matt, I think this is interesting because I am hearing more and more that CLIA inspectors are requiring these ranges on the chart. Is anyone else having CLIA inspectors ding them about operational humidity and temperature ranges on temperature charts? Some CLIA inspectors require room temperatures to be recorded as well. Everyone's input would be appreciated. Thanks. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matt Bancroft Sent: Thursday, March 20, 2008 5:04 AM To: Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: [Histonet] CLIA For those of you that have to go thru a CLIA inspeciton, I had mine yesterday and came across a issue, that in my 15 years of histology have never came across so I thought that I would share with you all. The inspector that I had opened up the owners manuals of my equipment (paraffin pot, water-bath, embedding center, etc) and looked at the manufactors recommendations for room temp and room humidity. She was going to ding me on not haveing those ranges on my QC chats, but I changed them while she was here. So if you do not have those ranges on your QC charts I would recommened it. Matt Bancroft Laboratory Manager --------------------------------- Never miss a thing. Make Yahoo your homepage. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kathy.Abels <@t> sial.com Thu Mar 20 10:01:28 2008 From: Kathy.Abels <@t> sial.com (Kathy Abels) Date: Thu Mar 20 10:02:06 2008 Subject: [Histonet] Kathy Abels/ops/diag/sial is out of the office. Message-ID: I will be out of the office starting 03/19/2008 and will not return until 03/25/2008. I will respond to your message when I return. For urgent matters, Please contact Roy Street.. This message and any files transmitted with it are the property of Sigma-Aldrich Corporation, are confidential, and are intended solely for the use of the person or entity to whom this e-mail is addressed. If you are not one of the named recipient(s) or otherwise have reason to believe that you have received this message in error, please contact the sender and delete this message immediately from your computer. Any other use, retention, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. From kemlo <@t> f2s.com Thu Mar 20 10:06:09 2008 From: kemlo <@t> f2s.com (kemlo) Date: Thu Mar 20 10:07:43 2008 Subject: AW: [Histonet] Kidney Bx question In-Reply-To: <5C0BED61F529364E86309CADEA63FEF20163F3A5@TRFT-EX01.xRothGen.nhs.uk> References: <5C0BED61F529364E86309CADEA63FEF20163F3A5@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <97B93FACD3C942AB928EC9BE28647705@KemloPC> Terry it doesn't translate!!! Men and women, Brits and Yanks, different species. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: 20 March 2008 14:32 To: Godfrey Guerzon; histoinfo@comcast.net; Gudrun Lang Cc: histonet@lists.utsouthwestern.edu Subject: RE: AW: [Histonet] Kidney Bx question Godfrey, Nobody has said anything snide, or put anybody down. Your post is typical of those lacking a sense of humour, and who think being professional is synonymous with being formal. It is not. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Godfrey Guerzon Sent: 20 March 2008 14:17 To: histoinfo@comcast.net; Gudrun Lang Cc: histonet@lists.utsouthwestern.edu Subject: Re: AW: [Histonet] Kidney Bx question We are supposed to be professionals. What we post are real issues in our situation and ask for help. Professionals don't make snide remarks and put down our colleagues. If we think that it will raise our stature as professionals when we make a joke and condescending comments on things that we need help on, we are greatly mistaken. A wise man once said "if you have nothing good to say about anything - shut up". We need to understand that each situation is different - who are we to judge? We post a real issue in our own situation and we don't need "smart" colleagues make a joke of our situation. Do we want to be treated as professionals - then let us communicate as professionals. No snide remarks or condescending comments - PLEASE!!! - Help and good ideas are welcome - if you have nothiing to contribute to the issue at hand - please don't waste your (and ours) valuable time with useless comments. Godfrey >>> "Gudrun Lang" 3/20/2008 9:53 AM >>> Would you be so kind and explain, why you have to do a short run. Are these indeed NTX-biopsies, that need a report as fast as possible? Or is it just the fear to over-process the biopsies? We have ntx from time to time, but even then, the clinicians wait for the report until the next day, noon. So we can process the biopsies with our regular histo and do the specialstains (Jones, PAS, Congored, EvG, SFOG = kind of trichrome), immunohistochemistry and immunofluorescence. We process the needlebiopsies in sponges and with the regular 13-hour protocol. Regarding IHC a fixation-time under 6 hours could also drive to problems. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von histoinfo@comcast.net Gesendet: Donnerstag, 20. M?rz 2008 14:01 An: Rene J Buesa; Marshall Terry Dr,Consultant Histopathologist; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] Kidney Bx question Rene, Yes they are agitated. And the tearing problem that I originally described is fairly infrequent. It's just that when it does happen we can't figure out why. When it does happen nothing that we can see is any different than any other day. Nothing in common as far as collection site either. I am beginning to think more and more that it may be on days that we have 5 or 6 bxs that are all run together. But even then it is not every bx. I think we will take better steps to prevent solution carryover and see what happens. Of course it is so infrequent that we have this problem that it will be months before we can say that we "fixed" the problem. I would still like to compare times with others out there though. So please share your times for hand processing. Thanks, Jennifer -------------- Original message -------------- From: Rene J Buesa For hand processing at room temperature that schedule is too short, unless the samples are agitated. Ren? J. "Marshall Terry Dr, Consultant Histopathologist" wrote: Do you have a train to catch? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histoinfo@comcast.net Sent: 19 March 2008 17:14 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kidney Bx question We have been having some trouble with the inner surface of the tubules looking torn look to the tissue. We hand process all our kidney bx's with the times as follows. 1-formalin 15 minutes 2-50% ethanol 15 minutes 3-70% ethanol 15 minutes 4-80% ethanol 15 minutes 5-95% ethanol 15 minutes 6-100% ethanol 15 minutes 7-100% ethanol 15 minutes 9-xylene 15 minutes 10-xylene 15 minutes Parraffin x2, 20 minutes each. They are then cut at 1 micron for PAS & JONES and 2 microns for H&E & TRICHROME. We are trying to determine if this tearing is an artifact from processing, collection or some other factor we haven't thought of. Has anyone else come across this or a similar problem. All thoughts and suggestions welcome. Jennifer Saunders HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ###################################################################### This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. ###################################################################### _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------------------------------- THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE ADDRESSEE LISTED ABOVE. This record has been disclosed in accordance with Subtitle 3 of Title 4 of the Health-General Article of the Annotated Code of Maryland. Further disclosure of medical information contained herein is prohibited. If you are neither the intended recipient nor the individual responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure of patient information is strictly prohibited. If you have received this email in error, immediately notify us by telephone or return email. ----------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Thu Mar 20 10:10:15 2008 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Thu Mar 20 10:10:50 2008 Subject: AW: [Histonet] Kidney Bx question In-Reply-To: <5C0BED61F529364E86309CADEA63FEF20163F3A5@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <979FF5962E234F45B06CF0DB7C1AABB21617F470@chi2k3ms01.columbuschildrens.net> And we wonder why people get so p*****d off with Histonet and unsubscribe for long periods of time................. Get a grip people! Take a pill and calm down! Terry & Kemlo, it was brought up previously about the difference in humor on both sides on the Atlantic. Even though many parts of the country are well exposed to British TV, especially British comedies, much of the humor is lost on them. There is also a great difference in demands placed on histology labs & personnel in the US. The turn-around-times that exist in the UK at present, just aren't tolerated over here. Is everything a bowl of cherries over here? Absolutely not, but staff have to work under the expectations and constraints that are placed/forced on them. To all the Americans that get involved and respond with outbursts - lighten up! Life is too short to send your blood pressure up 20 points over something as trivial as this. I feel competent to qualify these statements because I worked in Scotland for 18 years, 4 in Middle East, and 16 years in the US. Well I've said my piece; I'm signing off for another couple of months till peoples egos subside and we can get back to the purpose Histonet was established. Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 Ronald.Houston@NationwideChildrens.org Columbus Children's Hospital is now Nationwide Children's Hospital www.NationwideChildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Thursday, March 20, 2008 10:32 AM To: Godfrey Guerzon; histoinfo@comcast.net; Gudrun Lang Cc: histonet@lists.utsouthwestern.edu Subject: RE: AW: [Histonet] Kidney Bx question Godfrey, Nobody has said anything snide, or put anybody down. Your post is typical of those lacking a sense of humour, and who think being professional is synonymous with being formal. It is not. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Godfrey Guerzon Sent: 20 March 2008 14:17 To: histoinfo@comcast.net; Gudrun Lang Cc: histonet@lists.utsouthwestern.edu Subject: Re: AW: [Histonet] Kidney Bx question We are supposed to be professionals. What we post are real issues in our situation and ask for help. Professionals don't make snide remarks and put down our colleagues. If we think that it will raise our stature as professionals when we make a joke and condescending comments on things that we need help on, we are greatly mistaken. A wise man once said "if you have nothing good to say about anything - shut up". We need to understand that each situation is different - who are we to judge? We post a real issue in our own situation and we don't need "smart" colleagues make a joke of our situation. Do we want to be treated as professionals - then let us communicate as professionals. No snide remarks or condescending comments - PLEASE!!! - Help and good ideas are welcome - if you have nothiing to contribute to the issue at hand - please don't waste your (and ours) valuable time with useless comments. Godfrey >>> "Gudrun Lang" 3/20/2008 9:53 AM >>> Would you be so kind and explain, why you have to do a short run. Are these indeed NTX-biopsies, that need a report as fast as possible? Or is it just the fear to over-process the biopsies? We have ntx from time to time, but even then, the clinicians wait for the report until the next day, noon. So we can process the biopsies with our regular histo and do the specialstains (Jones, PAS, Congored, EvG, SFOG = kind of trichrome), immunohistochemistry and immunofluorescence. We process the needlebiopsies in sponges and with the regular 13-hour protocol. Regarding IHC a fixation-time under 6 hours could also drive to problems. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von histoinfo@comcast.net Gesendet: Donnerstag, 20. M?rz 2008 14:01 An: Rene J Buesa; Marshall Terry Dr,Consultant Histopathologist; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] Kidney Bx question Rene, Yes they are agitated. And the tearing problem that I originally described is fairly infrequent. It's just that when it does happen we can't figure out why. When it does happen nothing that we can see is any different than any other day. Nothing in common as far as collection site either. I am beginning to think more and more that it may be on days that we have 5 or 6 bxs that are all run together. But even then it is not every bx. I think we will take better steps to prevent solution carryover and see what happens. Of course it is so infrequent that we have this problem that it will be months before we can say that we "fixed" the problem. I would still like to compare times with others out there though. So please share your times for hand processing. Thanks, Jennifer -------------- Original message -------------- From: Rene J Buesa For hand processing at room temperature that schedule is too short, unless the samples are agitated. Ren? J. "Marshall Terry Dr, Consultant Histopathologist" wrote: Do you have a train to catch? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histoinfo@comcast.net Sent: 19 March 2008 17:14 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kidney Bx question We have been having some trouble with the inner surface of the tubules looking torn look to the tissue. We hand process all our kidney bx's with the times as follows. 1-formalin 15 minutes 2-50% ethanol 15 minutes 3-70% ethanol 15 minutes 4-80% ethanol 15 minutes 5-95% ethanol 15 minutes 6-100% ethanol 15 minutes 7-100% ethanol 15 minutes 9-xylene 15 minutes 10-xylene 15 minutes Parraffin x2, 20 minutes each. They are then cut at 1 micron for PAS & JONES and 2 microns for H&E & TRICHROME. We are trying to determine if this tearing is an artifact from processing, collection or some other factor we haven't thought of. Has anyone else come across this or a similar problem. All thoughts and suggestions welcome. Jennifer Saunders HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ###################################################################### This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. ###################################################################### _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------------------------------- THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE ADDRESSEE LISTED ABOVE. This record has been disclosed in accordance with Subtitle 3 of Title 4 of the Health-General Article of the Annotated Code of Maryland. Further disclosure of medical information contained herein is prohibited. If you are neither the intended recipient nor the individual responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure of patient information is strictly prohibited. If you have received this email in error, immediately notify us by telephone or return email. ----------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From HornHV <@t> archildrens.org Thu Mar 20 10:25:07 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Mar 20 10:26:48 2008 Subject: AW: [Histonet] Kidney Bx question In-Reply-To: References: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82B96@EMAIL.archildrens.org> Amen Godfrey I was thinking the same thing..... Jennifer, I would extend the processing times by about 5 minutes each and I would add one more formalin... Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Godfrey Guerzon Sent: Thursday, March 20, 2008 9:17 AM To: histoinfo@comcast.net; Gudrun Lang Cc: histonet@lists.utsouthwestern.edu Subject: Re: AW: [Histonet] Kidney Bx question We are supposed to be professionals. What we post are real issues in our situation and ask for help. Professionals don't make snide remarks and put down our colleagues. If we think that it will raise our stature as professionals when we make a joke and condescending comments on things that we need help on, we are greatly mistaken. A wise man once said "if you have nothing good to say about anything - shut up". We need to understand that each situation is different - who are we to judge? We post a real issue in our own situation and we don't need "smart" colleagues make a joke of our situation. Do we want to be treated as professionals - then let us communicate as professionals. No snide remarks or condescending comments - PLEASE!!! - Help and good ideas are welcome - if you have nothiing to contribute to the issue at hand - please don't waste your (and ours) valuable time with useless comments. Godfrey >>> "Gudrun Lang" 3/20/2008 9:53 AM >>> Would you be so kind and explain, why you have to do a short run. Are these indeed NTX-biopsies, that need a report as fast as possible? Or is it just the fear to over-process the biopsies? We have ntx from time to time, but even then, the clinicians wait for the report until the next day, noon. So we can process the biopsies with our regular histo and do the specialstains (Jones, PAS, Congored, EvG, SFOG = kind of trichrome), immunohistochemistry and immunofluorescence. We process the needlebiopsies in sponges and with the regular 13-hour protocol. Regarding IHC a fixation-time under 6 hours could also drive to problems. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von histoinfo@comcast.net Gesendet: Donnerstag, 20. M?rz 2008 14:01 An: Rene J Buesa; Marshall Terry Dr,Consultant Histopathologist; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] Kidney Bx question Rene, Yes they are agitated. And the tearing problem that I originally described is fairly infrequent. It's just that when it does happen we can't figure out why. When it does happen nothing that we can see is any different than any other day. Nothing in common as far as collection site either. I am beginning to think more and more that it may be on days that we have 5 or 6 bxs that are all run together. But even then it is not every bx. I think we will take better steps to prevent solution carryover and see what happens. Of course it is so infrequent that we have this problem that it will be months before we can say that we "fixed" the problem. I would still like to compare times with others out there though. So please share your times for hand processing. Thanks, Jennifer -------------- Original message -------------- From: Rene J Buesa For hand processing at room temperature that schedule is too short, unless the samples are agitated. Ren? J. "Marshall Terry Dr, Consultant Histopathologist" wrote: Do you have a train to catch? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histoinfo@comcast.net Sent: 19 March 2008 17:14 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kidney Bx question We have been having some trouble with the inner surface of the tubules looking torn look to the tissue. We hand process all our kidney bx's with the times as follows. 1-formalin 15 minutes 2-50% ethanol 15 minutes 3-70% ethanol 15 minutes 4-80% ethanol 15 minutes 5-95% ethanol 15 minutes 6-100% ethanol 15 minutes 7-100% ethanol 15 minutes 9-xylene 15 minutes 10-xylene 15 minutes Parraffin x2, 20 minutes each. They are then cut at 1 micron for PAS & JONES and 2 microns for H&E & TRICHROME. We are trying to determine if this tearing is an artifact from processing, collection or some other factor we haven't thought of. Has anyone else come across this or a similar problem. All thoughts and suggestions welcome. Jennifer Saunders HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ###################################################################### This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. ###################################################################### _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------------------------------- THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE ADDRESSEE LISTED ABOVE. This record has been disclosed in accordance with Subtitle 3 of Title 4 of the Health-General Article of the Annotated Code of Maryland. Further disclosure of medical information contained herein is prohibited. If you are neither the intended recipient nor the individual responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure of patient information is strictly prohibited. If you have received this email in error, immediately notify us by telephone or return email. ----------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histology.bc <@t> shaw.ca Thu Mar 20 10:36:23 2008 From: histology.bc <@t> shaw.ca (Paul Bradbury) Date: Thu Mar 20 10:31:39 2008 Subject: [Histonet] Train to catch?Yes! In-Reply-To: <257EFA089A03407B898BA8E9BB85FAAC@KemloPC> References: <032020081210.27739.47E25427000E910400006C5B221655140601000207019B9C0708@comcast.net> <257EFA089A03407B898BA8E9BB85FAAC@KemloPC> Message-ID: <47E28477.1030609@shaw.ca> The original problem as described, is almost certainly a fixation issue. I can appreciate the desire to provide a diagnosis as rapidly as possible, established practices still have to be followed. Treating any tissue, regardless of its size for 15 minutes in formalin, cannot and will not fix it. It is just not possible. It has been proven, many, many times by numerous workers, that adequate formalin fixation requires several hours even with tiny biopsies. Tissues that are "immersed" in formalin for 15 minutes and then transferred to alcohols are fixed by the alcohols, not by the formalin. Again, it has been shown repeatedly, that alcohol is not a good fixative for solid tissues. It is great for smears, blood films, aspirates, etc, but is a very poor choice for solid tissues, even tiny biopsies. I would hope that the clinical significance of the biopsy would encourage the technologists to employ procedures that would ensure an end-product of the highest quality. Speed and a rapid turn-around-time are admirable goals, but not at the expense of quality! I would strongly suggest that the current processing schedule be re-designed and that a much longer time be assigned to the fixation step. A second suggestion would be to use slightly warm formalin to increase the speed of fixation. Raising the temperature of the formalin to 37 degrees, will increase the speed of fixation noticeably. I would not suggest raising the temperature further without running some trial experiments first. As an after thought, I have read all the responses to this question, and I have not interpreted any of them as "snide or condescending". Several of them have been given with a "tongue in cheek" attitude, but that does not make them snide or condescending. I fully agree with Terry's comment that a very professional and helpful suggestion can be made with humor and without the need for formality. Anybody who subscribes to Histonet on a regular basis should know by now that some of the most valued contributors use a dry, English, sense of humor to present their suggestions. Paul Bradbury, Kamloops, Canada ,kemlo wrote: > 15 mins will not fix anything!!! That's your major issue > > Take care > > > > _____ > > From: histoinfo@comcast.net [mailto:histoinfo@comcast.net] > Sent: 20 March 2008 12:10 > To: kemlo; mtitford@aol.com; Histonet@pathology.swmed.edu > Subject: RE: [Histonet] Train to catch?Yes! > > > > I guess I am surprised at all the comments about this being such a rush > processing schedule. These are very small needle core bxs, and the > processing takes about 3 hours to complete. Is anyone else using hand > processing willing to share their protocol please? > > Jennifer > > > > -------------- Original message -------------- > From: "kemlo" > > >> Oh so renal biopsy stat!!! >> >> Is life really that hectic? Maybe a little more time spent gets the >> > correct > >> result rather than rushing and ruining. >> >> Tortoise and the hare? >> >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From Marissa <@t> labcareer.com Thu Mar 20 10:33:10 2008 From: Marissa <@t> labcareer.com (Marissa Perez) Date: Thu Mar 20 10:33:17 2008 Subject: AW: [Histonet] Kidney Bx question In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82B96@EMAIL.archildrens.org> References: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82B96@EMAIL.archildrens.org> Message-ID: <26A7728EF768DA478F45954252293C20DD821D@matrix.HCCI.local> Hello, My name is Marissa Perez and I am a recruiter here with HCCI. We specialize in lab staffing. Does anyone know of any facilities who are desperately in need of HTs? Best regards, Marissa Perez HealthCare Connections, Inc. Toll Free: (866) 346-8522 x 319 Fax: (954) 755-0819 E-mail: marissa@labcareer.com Website: www.labcareer.com If you would like to be removed from this mailing list, please click HERE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, March 20, 2008 10:25 AM To: Godfrey Guerzon; histoinfo@comcast.net; Gudrun Lang Cc: histonet@lists.utsouthwestern.edu Subject: RE: AW: [Histonet] Kidney Bx question Amen Godfrey I was thinking the same thing..... Jennifer, I would extend the processing times by about 5 minutes each and I would add one more formalin... Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Godfrey Guerzon Sent: Thursday, March 20, 2008 9:17 AM To: histoinfo@comcast.net; Gudrun Lang Cc: histonet@lists.utsouthwestern.edu Subject: Re: AW: [Histonet] Kidney Bx question We are supposed to be professionals. What we post are real issues in our situation and ask for help. Professionals don't make snide remarks and put down our colleagues. If we think that it will raise our stature as professionals when we make a joke and condescending comments on things that we need help on, we are greatly mistaken. A wise man once said "if you have nothing good to say about anything - shut up". We need to understand that each situation is different - who are we to judge? We post a real issue in our own situation and we don't need "smart" colleagues make a joke of our situation. Do we want to be treated as professionals - then let us communicate as professionals. No snide remarks or condescending comments - PLEASE!!! - Help and good ideas are welcome - if you have nothiing to contribute to the issue at hand - please don't waste your (and ours) valuable time with useless comments. Godfrey >>> "Gudrun Lang" 3/20/2008 9:53 AM >>> Would you be so kind and explain, why you have to do a short run. Are these indeed NTX-biopsies, that need a report as fast as possible? Or is it just the fear to over-process the biopsies? We have ntx from time to time, but even then, the clinicians wait for the report until the next day, noon. So we can process the biopsies with our regular histo and do the specialstains (Jones, PAS, Congored, EvG, SFOG = kind of trichrome), immunohistochemistry and immunofluorescence. We process the needlebiopsies in sponges and with the regular 13-hour protocol. Regarding IHC a fixation-time under 6 hours could also drive to problems. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von histoinfo@comcast.net Gesendet: Donnerstag, 20. M?rz 2008 14:01 An: Rene J Buesa; Marshall Terry Dr,Consultant Histopathologist; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] Kidney Bx question Rene, Yes they are agitated. And the tearing problem that I originally described is fairly infrequent. It's just that when it does happen we can't figure out why. When it does happen nothing that we can see is any different than any other day. Nothing in common as far as collection site either. I am beginning to think more and more that it may be on days that we have 5 or 6 bxs that are all run together. But even then it is not every bx. I think we will take better steps to prevent solution carryover and see what happens. Of course it is so infrequent that we have this problem that it will be months before we can say that we "fixed" the problem. I would still like to compare times with others out there though. So please share your times for hand processing. Thanks, Jennifer -------------- Original message -------------- From: Rene J Buesa For hand processing at room temperature that schedule is too short, unless the samples are agitated. Ren? J. "Marshall Terry Dr, Consultant Histopathologist" wrote: Do you have a train to catch? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histoinfo@comcast.net Sent: 19 March 2008 17:14 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kidney Bx question We have been having some trouble with the inner surface of the tubules looking torn look to the tissue. We hand process all our kidney bx's with the times as follows. 1-formalin 15 minutes 2-50% ethanol 15 minutes 3-70% ethanol 15 minutes 4-80% ethanol 15 minutes 5-95% ethanol 15 minutes 6-100% ethanol 15 minutes 7-100% ethanol 15 minutes 9-xylene 15 minutes 10-xylene 15 minutes Parraffin x2, 20 minutes each. They are then cut at 1 micron for PAS & JONES and 2 microns for H&E & TRICHROME. We are trying to determine if this tearing is an artifact from processing, collection or some other factor we haven't thought of. Has anyone else come across this or a similar problem. All thoughts and suggestions welcome. Jennifer Saunders HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ###################################################################### This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. ###################################################################### _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------------------------------- THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE ADDRESSEE LISTED ABOVE. This record has been disclosed in accordance with Subtitle 3 of Title 4 of the Health-General Article of the Annotated Code of Maryland. Further disclosure of medical information contained herein is prohibited. If you are neither the intended recipient nor the individual responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure of patient information is strictly prohibited. If you have received this email in error, immediately notify us by telephone or return email. ----------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tim.morken <@t> thermofisher.com Thu Mar 20 11:10:09 2008 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Thu Mar 20 11:10:55 2008 Subject: [Histonet] CLIA In-Reply-To: References: <1F937FB30BDB7C4A9F39F83FEA8D379F9F532C@bruexchange1.digestivespecialists.com><320362.54247.qm@web63401.mail.re1.yahoo.com> Message-ID: <6BFF6D137DF6BC43B33891BA96E83B190147F621@PGHCR-EXMB-VS-1.na.fshrnet.com> Adding the ranges certainly adds to the tidbits of information that could be used for troubleshooting., but it is humorous in that if the lab environment was outiside the temperature and humidity ranges given for most equipment, no one would be working in the lab and the equipment would most likely be off due the power failure that caused the temperature and humidity to be outside the ranges! Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mickie Johnson Sent: Thursday, March 20, 2008 7:07 AM To: 'Matt Bancroft'; 'Blazek, Linda'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CLIA Matt, I think this is interesting because I am hearing more and more that CLIA inspectors are requiring these ranges on the chart. Is anyone else having CLIA inspectors ding them about operational humidity and temperature ranges on temperature charts? Some CLIA inspectors require room temperatures to be recorded as well. Everyone's input would be appreciated. Thanks. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matt Bancroft Sent: Thursday, March 20, 2008 5:04 AM To: Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: [Histonet] CLIA For those of you that have to go thru a CLIA inspeciton, I had mine yesterday and came across a issue, that in my 15 years of histology have never came across so I thought that I would share with you all. The inspector that I had opened up the owners manuals of my equipment (paraffin pot, water-bath, embedding center, etc) and looked at the manufactors recommendations for room temp and room humidity. She was going to ding me on not haveing those ranges on my QC chats, but I changed them while she was here. So if you do not have those ranges on your QC charts I would recommened it. Matt Bancroft Laboratory Manager --------------------------------- Never miss a thing. Make Yahoo your homepage. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Thu Mar 20 11:25:24 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Mar 20 11:25:33 2008 Subject: AW: [Histonet] Train to catch?Yes! In-Reply-To: <032020081451.7799.47E279DB00073E3200001E77221655140601000207019B9C0708@comcast.net> Message-ID: <000f01c88aa7$011fec60$eeeea8c0@dielangs.at> The penetration-time will be a matter of size, but the cross-linking time won't. (Publications of Cecil Fox and Helander) I never heard of compression because of the VIP. Has anyone other? Isn't it again a matter of fixation? Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von histoinfo@comcast.net Gesendet: Donnerstag, 20. M?rz 2008 15:51 An: Victor Tobias; kemlo Cc: mtitford@aol.com; Histonet@pathology.swmed.edu Betreff: Re: [Histonet] Train to catch?Yes! The 15 minute formalin station is only used for same dayers. And mostly just for a little extra time in formalin with agitation if we don't know how long it has already been in formalin. Often when tissue comes to us fresh we will give it at least a half an hour. But remember these are very small needle cores, we are talking smaller than your average prostate bx. We don't process them on our bx VIP at the request of our pathologist due to compression of the tissue. This happens even if we turn off the vacuum. I have not been offended by anyones comments at all, rather enjoyed them to be honest. It is the comments by everyone that gives me the insight to look at what we do and how we do it with a different point of view. This is exactly what I am looking for when I post a question to the histonet. You are all wonderful and a great asset when trying to figure something out or looking for a new protocol. Thank You, Thank You, Jennifer -------------- Original message -------------- From: Victor Tobias > I don't think it has been established that the tissue is unfixed. Our > facility has a large renal service and our processing times are even > shorter, but we use an automated processor. Most of our cases are sent > to us so the tissue is fixed. > > Victor > > kemlo wrote: > > 15 mins will not fix anything!!! That's your major issue > > > > Take care > > > > > > > > _____ > > > > From: histoinfo@comcast.net [mailto:histoinfo@comcast.net] > > Sent: 20 March 2008 12:10 > > To: kemlo; mtitford@aol.com; Histonet@pathology.swmed.edu > > Subject: RE: [Histonet] Train to catch?Yes! > > > > > > > > I guess I am surprised at all the comments about this being such a rush > > processing schedule. These are very small needle core bxs, and the > > processing takes about 3 hours to complete. Is anyone else using hand > > processing willing to share their protocol please? > > > > Jennifer > > > > > > > > -------------- Original message -------------- > > From: "kemlo" > > > > > >> Oh so renal biopsy stat!!! > >> > >> Is life really that hectic? Maybe a little more time spent gets the > >> > > correct > > > >> result rather than rushing and ruining. > >> > >> Tortoise and the hare? > >> > >> > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- > Victor Tobias > Clinical Applications Analyst > University of Washington Medical Center > Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > victor@pathology.washington.edu > 206-598-2792 > 206-598-7659 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Thu Mar 20 11:29:08 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Mar 20 11:29:18 2008 Subject: [Histonet] air cleaning In-Reply-To: <1F937FB30BDB7C4A9F39F83FEA8D379F9F532C@bruexchange1.digestivespecialists.com> References: <1F937FB30BDB7C4A9F39F83FEA8D379F9F532C@bruexchange1.digestivespecialists.com> Message-ID: <5b6eb13e0803200929k2ad441a1jfac512967fbcf03a@mail.gmail.com> I think you're talking about something like the ionic breeze. It puts out a little UV light and makes oxygen go to ozone...and the ozone weighs down the dust in the air and makes it settle out (everywhere in the room). I think people decided this isn't as safe as they thought originally. I like HEPA filters because it takes the stuff out of the air and puts it in one place (on the filter, rather than everywhere in the room). Mark Tarango On Thu, Mar 20, 2008 at 4:12 AM, Blazek, Linda < lblazek@digestivespecialists.com> wrote: > Does anyone have any experience or know of something called UV air > cleaning for fumes in the histo lab? > > Thanks, > > Linda > > > > Linda Blazek HT (ASCP) > > Manager/Supervisor > > GI Pathology of Dayton > > 7415 Brandt Pike > > Huber Heights, OH 45424 > > Phone: (937) 293-4424 ext 7118 > > Email: lblazek@digestivespecialists.com > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rachelr <@t> mail.nih.gov Thu Mar 20 11:31:48 2008 From: rachelr <@t> mail.nih.gov (Rivka Rachel) Date: Thu Mar 20 11:32:07 2008 Subject: [Histonet] Looking for an experienced Histology, Immunohistochemistry biologist In-Reply-To: <26A7728EF768DA478F45954252293C20DD821D@matrix.HCCI.local> Message-ID: Hello, We will be posting for a Core Biologist position with the National Eye Institute in Bethesda, MD, and would like to know how likely it will be to find someone who has experience both with histology, including immunofluorscence and cryostat/vibratome sectioning, and with functional visual assessments such as ERG. Anyone interested? Rivka -- Rivka A. Rachel, MD, PhD Staff Scientist, National Eye Institute From lblazek <@t> digestivespecialists.com Thu Mar 20 11:46:39 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Mar 20 11:43:12 2008 Subject: [Histonet] CLIA In-Reply-To: <6BFF6D137DF6BC43B33891BA96E83B190147F621@PGHCR-EXMB-VS-1.na.fshrnet.com> References: <1F937FB30BDB7C4A9F39F83FEA8D379F9F532C@bruexchange1.digestivespecialists.com><320362.54247.qm@web63401.mail.re1.yahoo.com> <6BFF6D137DF6BC43B33891BA96E83B190147F621@PGHCR-EXMB-VS-1.na.fshrnet.com> Message-ID: <1F937FB30BDB7C4A9F39F83FEA8D379F9F533B@bruexchange1.digestivespecialists.com> After the first email about temps and humidity I checked all my operator manuals and found that if we are below 5 or over 40 C and over 60% humidity we would be out of range. I know I'm not working in that environment! Linda -----Original Message----- From: Morken, Tim [mailto:tim.morken@thermofisher.com] Sent: Thursday, March 20, 2008 12:10 PM To: mickie25@netzero.net; Matt Bancroft; Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CLIA Adding the ranges certainly adds to the tidbits of information that could be used for troubleshooting., but it is humorous in that if the lab environment was outiside the temperature and humidity ranges given for most equipment, no one would be working in the lab and the equipment would most likely be off due the power failure that caused the temperature and humidity to be outside the ranges! Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mickie Johnson Sent: Thursday, March 20, 2008 7:07 AM To: 'Matt Bancroft'; 'Blazek, Linda'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CLIA Matt, I think this is interesting because I am hearing more and more that CLIA inspectors are requiring these ranges on the chart. Is anyone else having CLIA inspectors ding them about operational humidity and temperature ranges on temperature charts? Some CLIA inspectors require room temperatures to be recorded as well. Everyone's input would be appreciated. Thanks. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matt Bancroft Sent: Thursday, March 20, 2008 5:04 AM To: Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: [Histonet] CLIA For those of you that have to go thru a CLIA inspeciton, I had mine yesterday and came across a issue, that in my 15 years of histology have never came across so I thought that I would share with you all. The inspector that I had opened up the owners manuals of my equipment (paraffin pot, water-bath, embedding center, etc) and looked at the manufactors recommendations for room temp and room humidity. She was going to ding me on not haveing those ranges on my QC chats, but I changed them while she was here. So if you do not have those ranges on your QC charts I would recommened it. Matt Bancroft Laboratory Manager --------------------------------- Never miss a thing. Make Yahoo your homepage. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AWeiss <@t> shorememorial.org Thu Mar 20 12:09:09 2008 From: AWeiss <@t> shorememorial.org (AWeiss@shorememorial.org) Date: Thu Mar 20 12:09:15 2008 Subject: [Histonet] Re: Histonet Digest, Vol 52, Issue 30 In-Reply-To: <20080320152806.454573C9968@smhex1.shorememorial.org> Message-ID: Matt, I think this is interesting because I am hearing more and more that CLIA inspectors are requiring these ranges on the chart. Is anyone else having CLIA inspectors ding them about operational humidity and temperature ranges on temperature charts? Some CLIA inspectors require room temperatures to be recorded as well. Everyone's input would be appreciated. Thanks. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 We do this monitoring for flow cytometry, there is a question for temp and humidy for CAP so we record this daily, with a monitor that records the temp 3x's./ day and the humidity. You can set the monitoring times for any interval you need. Then I download this monthly as part of the qc. Andrea J Weiss BST CT (ASCP) Cytotechnologist 609 653 3577 Ext 4907 aweiss@shorememorial.org From tjasper <@t> copc.net Thu Mar 20 12:12:21 2008 From: tjasper <@t> copc.net (Thomas Jasper) Date: Thu Mar 20 12:12:41 2008 Subject: AW: [Histonet] Kidney Bx question References: <5C0BED61F529364E86309CADEA63FEF20163F3A5@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <90354A475B420441B2A0396E5008D4965E207E@copc-sbs.COPC.local> Godfrey, Terry, Kemlo et al, I believe it was Eleanor Roosevelt who said, "No one can make you feel bad (unhappy, inferior, insert whatever negative word you like here) unless you allow them to!" I may not have the quote down exactly as stated but that is the gist of it. And unfortunately the world runs on perceptions. People are going to take things in whatever way they are perceived. This of course leads to misunderstandings, ruffled feathers etc. So, I must say Godfrey, I've been watching and posting the histonet for some time. I have followed many a thread involving Terry and Kemlo. They are both great resources, observant, knowledgable and yes...most of the time doing their best to inject levity whenever possible. And some folks don't get on with that. Not yet, having had the pleasure of personally meeting either of them, I think I can safely say that in no way would either of them have meant a comment to be an insult of any degree. I believe many people use humor as a way of looking at problems and then trying to determine solutions. Sometimes it is necessary to point something obvious (in this case TAT vs. quality) for the sake of bean counters, upper administrators or any other party that influences our work lives and yet doesn't fully understand where we're coming from. I am truly sorry if you feel this whole thing was insulting. I assure you I would not have. And please understand I'm not trying to apologize for anyone here, I'm just interested in fostering better understanding. Have a great day! Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr, Consultant Histopathologist Sent: Thursday, March 20, 2008 7:32 AM To: Godfrey Guerzon; histoinfo@comcast.net; Gudrun Lang Cc: histonet@lists.utsouthwestern.edu Subject: RE: AW: [Histonet] Kidney Bx question Godfrey, Nobody has said anything snide, or put anybody down. Your post is typical of those lacking a sense of humour, and who think being professional is synonymous with being formal. It is not. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Godfrey Guerzon Sent: 20 March 2008 14:17 To: histoinfo@comcast.net; Gudrun Lang Cc: histonet@lists.utsouthwestern.edu Subject: Re: AW: [Histonet] Kidney Bx question We are supposed to be professionals. What we post are real issues in our situation and ask for help. Professionals don't make snide remarks and put down our colleagues. If we think that it will raise our stature as professionals when we make a joke and condescending comments on things that we need help on, we are greatly mistaken. A wise man once said "if you have nothing good to say about anything - shut up". We need to understand that each situation is different - who are we to judge? We post a real issue in our own situation and we don't need "smart" colleagues make a joke of our situation. Do we want to be treated as professionals - then let us communicate as professionals. No snide remarks or condescending comments - PLEASE!!! - Help and good ideas are welcome - if you have nothiing to contribute to the issue at hand - please don't waste your (and ours) valuable time with useless comments. Godfrey >>> "Gudrun Lang" 3/20/2008 9:53 AM >>> Would you be so kind and explain, why you have to do a short run. Are these indeed NTX-biopsies, that need a report as fast as possible? Or is it just the fear to over-process the biopsies? We have ntx from time to time, but even then, the clinicians wait for the report until the next day, noon. So we can process the biopsies with our regular histo and do the specialstains (Jones, PAS, Congored, EvG, SFOG = kind of trichrome), immunohistochemistry and immunofluorescence. We process the needlebiopsies in sponges and with the regular 13-hour protocol. Regarding IHC a fixation-time under 6 hours could also drive to problems. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von histoinfo@comcast.net Gesendet: Donnerstag, 20. M?rz 2008 14:01 An: Rene J Buesa; Marshall Terry Dr,Consultant Histopathologist; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] Kidney Bx question Rene, Yes they are agitated. And the tearing problem that I originally described is fairly infrequent. It's just that when it does happen we can't figure out why. When it does happen nothing that we can see is any different than any other day. Nothing in common as far as collection site either. I am beginning to think more and more that it may be on days that we have 5 or 6 bxs that are all run together. But even then it is not every bx. I think we will take better steps to prevent solution carryover and see what happens. Of course it is so infrequent that we have this problem that it will be months before we can say that we "fixed" the problem. I would still like to compare times with others out there though. So please share your times for hand processing. Thanks, Jennifer -------------- Original message -------------- From: Rene J Buesa For hand processing at room temperature that schedule is too short, unless the samples are agitated. Ren? J. "Marshall Terry Dr, Consultant Histopathologist" wrote: Do you have a train to catch? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histoinfo@comcast.net Sent: 19 March 2008 17:14 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kidney Bx question We have been having some trouble with the inner surface of the tubules looking torn look to the tissue. We hand process all our kidney bx's with the times as follows. 1-formalin 15 minutes 2-50% ethanol 15 minutes 3-70% ethanol 15 minutes 4-80% ethanol 15 minutes 5-95% ethanol 15 minutes 6-100% ethanol 15 minutes 7-100% ethanol 15 minutes 9-xylene 15 minutes 10-xylene 15 minutes Parraffin x2, 20 minutes each. They are then cut at 1 micron for PAS & JONES and 2 microns for H&E & TRICHROME. We are trying to determine if this tearing is an artifact from processing, collection or some other factor we haven't thought of. Has anyone else come across this or a similar problem. All thoughts and suggestions welcome. Jennifer Saunders HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ###################################################################### This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. ###################################################################### _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------------------------------- THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE ADDRESSEE LISTED ABOVE. This record has been disclosed in accordance with Subtitle 3 of Title 4 of the Health-General Article of the Annotated Code of Maryland. Further disclosure of medical information contained herein is prohibited. If you are neither the intended recipient nor the individual responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure of patient information is strictly prohibited. If you have received this email in error, immediately notify us by telephone or return email. ----------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Thu Mar 20 12:20:20 2008 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Mar 20 12:20:24 2008 Subject: [Histonet] Re: Histonet Digest, Vol 52, Issue 30 In-Reply-To: Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F549@lmhsmail.lmhealth.org> I had a JCAHO inspector tell me about 6 years ago that I should have the temperature ranges on the charts but have never had anyone suggest the operational humidity... Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of AWeiss@shorememorial.org Sent: Thursday, March 20, 2008 1:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 52, Issue 30 Matt, I think this is interesting because I am hearing more and more that CLIA inspectors are requiring these ranges on the chart. Is anyone else having CLIA inspectors ding them about operational humidity and temperature ranges on temperature charts? Some CLIA inspectors require room temperatures to be recorded as well. Everyone's input would be appreciated. Thanks. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 We do this monitoring for flow cytometry, there is a question for temp and humidy for CAP so we record this daily, with a monitor that records the temp 3x's./ day and the humidity. You can set the monitoring times for any interval you need. Then I download this monthly as part of the qc. Andrea J Weiss BST CT (ASCP) Cytotechnologist 609 653 3577 Ext 4907 aweiss@shorememorial.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mohs76009 <@t> yahoo.com Thu Mar 20 12:25:17 2008 From: mohs76009 <@t> yahoo.com (Matt Bancroft) Date: Thu Mar 20 12:25:23 2008 Subject: [Histonet] CLIA In-Reply-To: <1F937FB30BDB7C4A9F39F83FEA8D379F9F533B@bruexchange1.digestivespecialists.com> Message-ID: <785069.1345.qm@web63411.mail.re1.yahoo.com> Becareful, they will ding on it. I think that it is crazy. I am lucky and eveything was in specks but she was hunting for something "Blazek, Linda" wrote: After the first email about temps and humidity I checked all my operator manuals and found that if we are below 5 or over 40 C and over 60% humidity we would be out of range. I know I'm not working in that environment! Linda -----Original Message----- From: Morken, Tim [mailto:tim.morken@thermofisher.com] Sent: Thursday, March 20, 2008 12:10 PM To: mickie25@netzero.net; Matt Bancroft; Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CLIA Adding the ranges certainly adds to the tidbits of information that could be used for troubleshooting., but it is humorous in that if the lab environment was outiside the temperature and humidity ranges given for most equipment, no one would be working in the lab and the equipment would most likely be off due the power failure that caused the temperature and humidity to be outside the ranges! Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mickie Johnson Sent: Thursday, March 20, 2008 7:07 AM To: 'Matt Bancroft'; 'Blazek, Linda'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CLIA Matt, I think this is interesting because I am hearing more and more that CLIA inspectors are requiring these ranges on the chart. Is anyone else having CLIA inspectors ding them about operational humidity and temperature ranges on temperature charts? Some CLIA inspectors require room temperatures to be recorded as well. Everyone's input would be appreciated. Thanks. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matt Bancroft Sent: Thursday, March 20, 2008 5:04 AM To: Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: [Histonet] CLIA For those of you that have to go thru a CLIA inspeciton, I had mine yesterday and came across a issue, that in my 15 years of histology have never came across so I thought that I would share with you all. The inspector that I had opened up the owners manuals of my equipment (paraffin pot, water-bath, embedding center, etc) and looked at the manufactors recommendations for room temp and room humidity. She was going to ding me on not haveing those ranges on my QC chats, but I changed them while she was here. So if you do not have those ranges on your QC charts I would recommened it. Matt Bancroft Laboratory Manager --------------------------------- Never miss a thing. Make Yahoo your homepage. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From vazquezr <@t> ohsu.edu Thu Mar 20 13:31:17 2008 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Thu Mar 20 13:31:45 2008 Subject: AW: [Histonet] Kidney Bx question Message-ID: Thomas well said!!! And excuse me, but I have a light rail to catch...:D+=== Robyn OHSU Portland, OR >>> "Thomas Jasper" 3/20/2008 10:12 AM >>> Godfrey, Terry, Kemlo et al, I believe it was Eleanor Roosevelt who said, "No one can make you feel bad (unhappy, inferior, insert whatever negative word you like here) unless you allow them to!" I may not have the quote down exactly as stated but that is the gist of it. And unfortunately the world runs on perceptions. People are going to take things in whatever way they are perceived. This of course leads to misunderstandings, ruffled feathers etc. So, I must say Godfrey, I've been watching and posting the histonet for some time. I have followed many a thread involving Terry and Kemlo. They are both great resources, observant, knowledgable and yes...most of the time doing their best to inject levity whenever possible. And some folks don't get on with that. Not yet, having had the pleasure of personally meeting either of them, I think I can safely say that in no way would either of them have meant a comment to be an insult of any degree. I believe many people use humor as a way of looking at problems and then trying to determine solutions. Sometimes it is necessary to point something obvious (in this case TAT vs. quality) for the sake of bean counters, upper administrators or any other party that influences our work lives and yet doesn't fully understand where we're coming from. I am truly sorry if you feel this whole thing was insulting. I assure you I would not have. And please understand I'm not trying to apologize for anyone here, I'm just interested in fostering better understanding. Have a great day! Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr, Consultant Histopathologist Sent: Thursday, March 20, 2008 7:32 AM To: Godfrey Guerzon; histoinfo@comcast.net; Gudrun Lang Cc: histonet@lists.utsouthwestern.edu Subject: RE: AW: [Histonet] Kidney Bx question Godfrey, Nobody has said anything snide, or put anybody down. Your post is typical of those lacking a sense of humour, and who think being professional is synonymous with being formal. It is not. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Godfrey Guerzon Sent: 20 March 2008 14:17 To: histoinfo@comcast.net; Gudrun Lang Cc: histonet@lists.utsouthwestern.edu Subject: Re: AW: [Histonet] Kidney Bx question We are supposed to be professionals. What we post are real issues in our situation and ask for help. Professionals don't make snide remarks and put down our colleagues. If we think that it will raise our stature as professionals when we make a joke and condescending comments on things that we need help on, we are greatly mistaken. A wise man once said "if you have nothing good to say about anything - shut up". We need to understand that each situation is different - who are we to judge? We post a real issue in our own situation and we don't need "smart" colleagues make a joke of our situation. Do we want to be treated as professionals - then let us communicate as professionals. No snide remarks or condescending comments - PLEASE!!! - Help and good ideas are welcome - if you have nothiing to contribute to the issue at hand - please don't waste your (and ours) valuable time with useless comments. Godfrey >>> "Gudrun Lang" 3/20/2008 9:53 AM >>> Would you be so kind and explain, why you have to do a short run. Are these indeed NTX-biopsies, that need a report as fast as possible? Or is it just the fear to over-process the biopsies? We have ntx from time to time, but even then, the clinicians wait for the report until the next day, noon. So we can process the biopsies with our regular histo and do the specialstains (Jones, PAS, Congored, EvG, SFOG = kind of trichrome), immunohistochemistry and immunofluorescence. We process the needlebiopsies in sponges and with the regular 13-hour protocol. Regarding IHC a fixation-time under 6 hours could also drive to problems. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von histoinfo@comcast.net Gesendet: Donnerstag, 20. M?rz 2008 14:01 An: Rene J Buesa; Marshall Terry Dr,Consultant Histopathologist; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] Kidney Bx question Rene, Yes they are agitated. And the tearing problem that I originally described is fairly infrequent. It's just that when it does happen we can't figure out why. When it does happen nothing that we can see is any different than any other day. Nothing in common as far as collection site either. I am beginning to think more and more that it may be on days that we have 5 or 6 bxs that are all run together. But even then it is not every bx. I think we will take better steps to prevent solution carryover and see what happens. Of course it is so infrequent that we have this problem that it will be months before we can say that we "fixed" the problem. I would still like to compare times with others out there though. So please share your times for hand processing. Thanks, Jennifer -------------- Original message -------------- From: Rene J Buesa For hand processing at room temperature that schedule is too short, unless the samples are agitated. Ren? J. "Marshall Terry Dr, Consultant Histopathologist" wrote: Do you have a train to catch? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histoinfo@comcast.net Sent: 19 March 2008 17:14 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kidney Bx question We have been having some trouble with the inner surface of the tubules looking torn look to the tissue. We hand process all our kidney bx's with the times as follows. 1-formalin 15 minutes 2-50% ethanol 15 minutes 3-70% ethanol 15 minutes 4-80% ethanol 15 minutes 5-95% ethanol 15 minutes 6-100% ethanol 15 minutes 7-100% ethanol 15 minutes 9-xylene 15 minutes 10-xylene 15 minutes Parraffin x2, 20 minutes each. They are then cut at 1 micron for PAS & JONES and 2 microns for H&E & TRICHROME. We are trying to determine if this tearing is an artifact from processing, collection or some other factor we haven't thought of. Has anyone else come across this or a similar problem. All thoughts and suggestions welcome. Jennifer Saunders HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ###################################################################### This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. ###################################################################### _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------------------------------- THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE ADDRESSEE LISTED ABOVE. This record has been disclosed in accordance with Subtitle 3 of Title 4 of the Health-General Article of the Annotated Code of Maryland. Further disclosure of medical information contained herein is prohibited. If you are neither the intended recipient nor the individual responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure of patient information is strictly prohibited. If you have received this email in error, immediately notify us by telephone or return email. ----------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Margaret.Perry <@t> sdstate.edu Thu Mar 20 13:44:12 2008 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Thu Mar 20 13:44:18 2008 Subject: [Histonet] CLIA Message-ID: How do you measure the temp and humidity? Are the instruments calibrated? I'm asking because this has been a QC issue with our QC manager. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 From elizabeth.heimrich <@t> bms.com Thu Mar 20 14:03:34 2008 From: elizabeth.heimrich <@t> bms.com (Elizabeth M Heimrich) Date: Thu Mar 20 14:03:46 2008 Subject: [Histonet] Re: Inspectors/ CAP JCAHO CLIA In-Reply-To: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F549@lmhsmail.lmhealth.org> References: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F549@lmhsmail.lmhealth.org> Message-ID: <47E2B506.3090100@bms.com> This whole thread really makes me wonder...does anyone have common sense anymore? Are these 'inspectors' just looking to ding the labs so that they look better to their superiors? Where is it going to stop? Next they'll want the temp ranges of the refrigerators each time the door is opened. What does the air temp and humidity really have to do with the quality of the service being provided? Do these minscule details make one lab better than another? I think not. You can become so caught up in these inspections that you make more mistakes regarding the patients' details. I think they are missing the point! It is just another check off on the list for the paper pushers. I am really glad I do not work in a hospital/ clinical setting. To all my fellow histonetters in these settings; my hat is off to you, for being able to perform your duties well, and deal with all the BS surrounding inspections you deserve a lot more than what the average salary is. I feel for you all!!! Beth Tom McNemar wrote: >I had a JCAHO inspector tell me about 6 years ago that I should have the temperature ranges on the charts but have never had anyone suggest the operational humidity... > >Tom McNemar, HT(ASCP) >Histology Co-ordinator >Licking Memorial Health Systems >(740) 348-4163 >(740) 348-4166 >tmcnemar@lmhealth.org >www.LMHealth.org > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of >AWeiss@shorememorial.org >Sent: Thursday, March 20, 2008 1:09 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Re: Histonet Digest, Vol 52, Issue 30 > > >Matt, > >I think this is interesting because I am hearing more and more that CLIA >inspectors are requiring these ranges on the chart. Is anyone else having >CLIA inspectors ding them about operational humidity and temperature ranges >on temperature charts? Some CLIA inspectors require room temperatures to be >recorded as well. Everyone's input would be appreciated. Thanks. > >Mickie > >Mickie Johnson, B.S., HTL(ASCP) >Mohs Histology Consulting Services, LLC > & Mohs Lab Staffing >2507 S. Manito Blvd. >Spokane, WA 99203 >509-954-7134 >FAX 509-624-3926 > > >We do this monitoring for flow cytometry, there is a question for temp and >humidy for CAP so we record this daily, with a monitor that records the >temp 3x's./ day and the humidity. You can set the monitoring times for any >interval you need. Then I download this monthly as part of the qc. > > >Andrea J Weiss BST CT (ASCP) >Cytotechnologist >609 653 3577 Ext 4907 >aweiss@shorememorial.org > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From TownsendD <@t> childrensdayton.org Thu Mar 20 14:13:16 2008 From: TownsendD <@t> childrensdayton.org (Dolores Townsend) Date: Thu Mar 20 14:13:45 2008 Subject: [Histonet] Re: Inspectors/ CAP JCAHO CLIA Message-ID: We were inspected by CAP last October, and the inspector who did histology said it best: - I have to report you for something, or "they" are going to wonder "What were you doing? Sitting around eating bonbons?" Sometimes, it's just better to have a little something wrong so they'll stop looking. No matter how hard you try, "they" will keep going until they have something to report. No matter if it has nothing to do with patient care or employee safety. Dolores From marktarango <@t> gmail.com Thu Mar 20 14:23:16 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Mar 20 14:23:20 2008 Subject: [Histonet] Re: Inspectors/ CAP JCAHO CLIA In-Reply-To: <47E2B506.3090100@bms.com> References: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F549@lmhsmail.lmhealth.org> <47E2B506.3090100@bms.com> Message-ID: <5b6eb13e0803201223g27869e8fw6ebaa02583d4c71d@mail.gmail.com> I agree with you 100%. Looking at all these tiny details... inspectors usually miss the big stuff. Many labs even have a strategy of letting the inspectors find and ding them on the little stuff, so they can distract inspectors from finding the real problems. Inspectors should stick to the stuff that actually matters. On Thu, Mar 20, 2008 at 12:03 PM, Elizabeth M Heimrich < elizabeth.heimrich@bms.com> wrote: > This whole thread really makes me wonder...does anyone have common sense > anymore? Are these 'inspectors' just looking to ding the labs so that > they look better to their superiors? Where is it going to stop? Next > they'll want the temp ranges of the refrigerators each time the door is > opened. What does the air temp and humidity really have to do with the > quality of the service being provided? Do these minscule details make > one lab better than another? I think not. You can become so caught up > in these inspections that you make more mistakes regarding the patients' > details. I think they are missing the point! It is just another check > off on the list for the paper pushers. I am really glad I do not work > in a hospital/ clinical setting. To all my fellow histonetters in these > settings; my hat is off to you, for being able to perform your duties > well, and deal with all the BS surrounding inspections you deserve a lot > more than what the average salary is. > I feel for you all!!! > Beth > > Tom McNemar wrote: > > >I had a JCAHO inspector tell me about 6 years ago that I should have the > temperature ranges on the charts but have never had anyone suggest the > operational humidity... > > > >Tom McNemar, HT(ASCP) > >Histology Co-ordinator > >Licking Memorial Health Systems > >(740) 348-4163 > >(740) 348-4166 > >tmcnemar@lmhealth.org > >www.LMHealth.org > > > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of > >AWeiss@shorememorial.org > >Sent: Thursday, March 20, 2008 1:09 PM > >To: histonet@lists.utsouthwestern.edu > >Subject: [Histonet] Re: Histonet Digest, Vol 52, Issue 30 > > > > > >Matt, > > > >I think this is interesting because I am hearing more and more that CLIA > >inspectors are requiring these ranges on the chart. Is anyone else having > >CLIA inspectors ding them about operational humidity and temperature > ranges > >on temperature charts? Some CLIA inspectors require room temperatures to > be > >recorded as well. Everyone's input would be appreciated. Thanks. > > > >Mickie > > > >Mickie Johnson, B.S., HTL(ASCP) > >Mohs Histology Consulting Services, LLC > > & Mohs Lab Staffing > >2507 S. Manito Blvd. > >Spokane, WA 99203 > >509-954-7134 > >FAX 509-624-3926 > > > > > >We do this monitoring for flow cytometry, there is a question for temp > and > >humidy for CAP so we record this daily, with a monitor that records the > >temp 3x's./ day and the humidity. You can set the monitoring times for > any > >interval you need. Then I download this monthly as part of the qc. > > > > > >Andrea J Weiss BST CT (ASCP) > >Cytotechnologist > >609 653 3577 Ext 4907 > >aweiss@shorememorial.org > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From CIngles <@t> uwhealth.org Thu Mar 20 14:29:46 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Thu Mar 20 14:29:51 2008 Subject: [Histonet] CLIA References: <1F937FB30BDB7C4A9F39F83FEA8D379F9F532C@bruexchange1.digestivespecialists.com><320362.54247.qm@web63401.mail.re1.yahoo.com> Message-ID: <08A0A863637F1349BBFD83A96B27A50A120107@uwhis-xchng3.uwhis.hosp.wisc.edu> Being a bigger hospital system, we have a whole department centered on CLIA/JCAHO inspections. They are a bit paranoid about being continually ready for the inspectors. (even though we just had our inspection in January) So we were prepared with all our QA sheets when they got here. We've even had to start keeping a log of lot #'s of the reagents that come into the lab. I feel checklisted to death some days. We had always kept a range on the machine temperature logs, but started keeping track of room temp/humidity a few months before the inspection. Anyway I believe they did look at our daily logs for temp/humidity stats. It feels nice when you can actually be one step ahead of the inspectors. Claire ________________________________ I think this is interesting because I am hearing more and more that CLIA inspectors are requiring these ranges on the chart. Is anyone else having CLIA inspectors ding them about operational humidity and temperature ranges on temperature charts? Some CLIA inspectors require room temperatures to be recorded as well. Everyone's input would be appreciated. Thanks. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net From JWEEMS <@t> sjha.org Thu Mar 20 14:43:17 2008 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Thu Mar 20 14:43:43 2008 Subject: AW: [Histonet] Kidney Bx question In-Reply-To: <90354A475B420441B2A0396E5008D4965E207E@copc-sbs.COPC.local> References: <5C0BED61F529364E86309CADEA63FEF20163F3A5@TRFT-EX01.xRothGen.nhs.uk> <90354A475B420441B2A0396E5008D4965E207E@copc-sbs.COPC.local> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD320518EAFF@sjhaexc02.sjha.org> Very well said, Thomas. Thanks, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Thursday, March 20, 2008 1:12 PM To: Marshall Terry Dr, Consultant Histopathologist Cc: histonet@lists.utsouthwestern.edu Subject: RE: AW: [Histonet] Kidney Bx question Godfrey, Terry, Kemlo et al, I believe it was Eleanor Roosevelt who said, "No one can make you feel bad (unhappy, inferior, insert whatever negative word you like here) unless you allow them to!" I may not have the quote down exactly as stated but that is the gist of it. And unfortunately the world runs on perceptions. People are going to take things in whatever way they are perceived. This of course leads to misunderstandings, ruffled feathers etc. So, I must say Godfrey, I've been watching and posting the histonet for some time. I have followed many a thread involving Terry and Kemlo. They are both great resources, observant, knowledgable and yes...most of the time doing their best to inject levity whenever possible. And some folks don't get on with that. Not yet, having had the pleasure of personally meeting either of them, I think I can safely say that in no way would either of them have meant a comment to be an insult of any degree. I believe many people use humor as a way of looking at problems and then trying to determine solutions. Sometimes it is necessary to point something obvious (in this case TAT vs. quality) for the sake of bean counters, upper administrators or any other party that influences our work lives and yet doesn't fully understand where we're coming from. I am truly sorry if you feel this whole thing was insulting. I assure you I would not have. And please understand I'm not trying to apologize for anyone here, I'm just interested in fostering better understanding. Have a great day! Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr, Consultant Histopathologist Sent: Thursday, March 20, 2008 7:32 AM To: Godfrey Guerzon; histoinfo@comcast.net; Gudrun Lang Cc: histonet@lists.utsouthwestern.edu Subject: RE: AW: [Histonet] Kidney Bx question Godfrey, Nobody has said anything snide, or put anybody down. Your post is typical of those lacking a sense of humour, and who think being professional is synonymous with being formal. It is not. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Godfrey Guerzon Sent: 20 March 2008 14:17 To: histoinfo@comcast.net; Gudrun Lang Cc: histonet@lists.utsouthwestern.edu Subject: Re: AW: [Histonet] Kidney Bx question We are supposed to be professionals. What we post are real issues in our situation and ask for help. Professionals don't make snide remarks and put down our colleagues. If we think that it will raise our stature as professionals when we make a joke and condescending comments on things that we need help on, we are greatly mistaken. A wise man once said "if you have nothing good to say about anything - shut up". We need to understand that each situation is different - who are we to judge? We post a real issue in our own situation and we don't need "smart" colleagues make a joke of our situation. Do we want to be treated as professionals - then let us communicate as professionals. No snide remarks or condescending comments - PLEASE!!! - Help and good ideas are welcome - if you have nothiing to contribute to the issue at hand - please don't waste your (and ours) valuable time with useless comments. Godfrey >>> "Gudrun Lang" 3/20/2008 9:53 AM >>> Would you be so kind and explain, why you have to do a short run. Are these indeed NTX-biopsies, that need a report as fast as possible? Or is it just the fear to over-process the biopsies? We have ntx from time to time, but even then, the clinicians wait for the report until the next day, noon. So we can process the biopsies with our regular histo and do the specialstains (Jones, PAS, Congored, EvG, SFOG = kind of trichrome), immunohistochemistry and immunofluorescence. We process the needlebiopsies in sponges and with the regular 13-hour protocol. Regarding IHC a fixation-time under 6 hours could also drive to problems. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von histoinfo@comcast.net Gesendet: Donnerstag, 20. M?rz 2008 14:01 An: Rene J Buesa; Marshall Terry Dr,Consultant Histopathologist; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] Kidney Bx question Rene, Yes they are agitated. And the tearing problem that I originally described is fairly infrequent. It's just that when it does happen we can't figure out why. When it does happen nothing that we can see is any different than any other day. Nothing in common as far as collection site either. I am beginning to think more and more that it may be on days that we have 5 or 6 bxs that are all run together. But even then it is not every bx. I think we will take better steps to prevent solution carryover and see what happens. Of course it is so infrequent that we have this problem that it will be months before we can say that we "fixed" the problem. I would still like to compare times with others out there though. So please share your times for hand processing. Thanks, Jennifer -------------- Original message -------------- From: Rene J Buesa For hand processing at room temperature that schedule is too short, unless the samples are agitated. Ren? J. "Marshall Terry Dr, Consultant Histopathologist" wrote: Do you have a train to catch? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histoinfo@comcast.net Sent: 19 March 2008 17:14 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kidney Bx question We have been having some trouble with the inner surface of the tubules looking torn look to the tissue. We hand process all our kidney bx's with the times as follows. 1-formalin 15 minutes 2-50% ethanol 15 minutes 3-70% ethanol 15 minutes 4-80% ethanol 15 minutes 5-95% ethanol 15 minutes 6-100% ethanol 15 minutes 7-100% ethanol 15 minutes 9-xylene 15 minutes 10-xylene 15 minutes Parraffin x2, 20 minutes each. They are then cut at 1 micron for PAS & JONES and 2 microns for H&E & TRICHROME. We are trying to determine if this tearing is an artifact from processing, collection or some other factor we haven't thought of. Has anyone else come across this or a similar problem. All thoughts and suggestions welcome. Jennifer Saunders HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ###################################################################### This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. ###################################################################### _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------------------------------- THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE ADDRESSEE LISTED ABOVE. This record has been disclosed in accordance with Subtitle 3 of Title 4 of the Health-General Article of the Annotated Code of Maryland. Further disclosure of medical information contained herein is prohibited. If you are neither the intended recipient nor the individual responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure of patient information is strictly prohibited. If you have received this email in error, immediately notify us by telephone or return email. ----------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From sccrshlly <@t> yahoo.com Thu Mar 20 13:45:55 2008 From: sccrshlly <@t> yahoo.com (Shelly Coker) Date: Thu Mar 20 14:46:05 2008 Subject: [Histonet] RE: Kidney Bx question Message-ID: <97641.26500.qm@web90308.mail.mud.yahoo.com> Not sure if this could be your problem or not, but we were experiencing some of the "torn" look to our GI biopsies for some time. It came down to our alcohols had too much water carryover. We process with a microwave, and the water basically boiled out of the tissue, "shredding" the components. We now change our alcohols after each run, not every other run and have not had problems since. If you are using any heat in your processing, this could be something to look at. Good luck! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histoinfo@comcast.net Sent: 19 March 2008 17:14 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kidney Bx question We have been having some trouble with the inner surface of the tubules looking torn look to the tissue. We hand process all our kidney bx's with the times as follows. 1-formalin 15 minutes 2-50% ethanol 15 minutes 3-70% ethanol 15 minutes 4-80% ethanol 15 minutes 5-95% ethanol 15 minutes 6-100% ethanol 15 minutes 7-100% ethanol 15 minutes 9-xylene 15 minutes 10-xylene 15 minutes Parraffin x2, 20 minutes each. They are then cut at 1 micron for PAS & JONES and 2 microns for H&E & TRICHROME. We are trying to determine if this tearing is an artifact from processing, collection or some other factor we haven't thought of. Has anyone else come across this or a similar problem. All thoughts and suggestions welcome. Jennifer Saunders HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ###################################################################### This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. ###################################################################### _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------------------------------- THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE ADDRESSEE LISTED ABOVE. This record has been disclosed in accordance with Subtitle 3 of Title 4 of the Health-General Article of the Annotated Code of Maryland. Further disclosure of medical information contained herein is prohibited. If you are neither the intended recipient nor the individual responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure of patient information is strictly prohibited. If you have received this email in error, immediately notify us by telephone or return email. ----------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From: "Houston, Ronald" Subject: RE: AW: [Histonet] Kidney Bx question CC: histonet@lists.utsouthwestern.edu Date: Thu, 20 Mar 2008 11:10:15 -0400 To: "Marshall Terry Dr, Consultant Histopathologist" , "Godfrey Guerzon" , , "Gudrun Lang" And we wonder why people get so p*****d off with Histonet and unsubscribe for long periods of time................. Get a grip people! Take a pill and calm down! Terry & Kemlo, it was brought up previously about the difference in humor on both sides on the Atlantic. Even though many parts of the country are well exposed to British TV, especially British comedies, much of the humor is lost on them. There is also a great difference in demands placed on histology labs & personnel in the US. The turn-around-times that exist in the UK at present, just aren't tolerated over here. Is everything a bowl of cherries over here? Absolutely not, but staff have to work under the expectations and constraints that are placed/forced on them. To all the Americans that get involved and respond with outbursts - lighten up! Life is too short to send your blood pressure up 20 points over something as trivial as this. I feel competent to qualify these statements because I worked in Scotland for 18 years, 4 in Middle East, and 16 years in the US. Well I've said my piece; I'm signing off for another couple of months till peoples egos subside and we can get back to the purpose Histonet was established. Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 Ronald.Houston@NationwideChildrens.org Columbus Children's Hospital is now Nationwide Children's Hospital www.NationwideChildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Thursday, March 20, 2008 10:32 AM To: Godfrey Guerzon; histoinfo@comcast.net; Gudrun Lang Cc: histonet@lists.utsouthwestern.edu Subject: RE: AW: [Histonet] Kidney Bx question Godfrey, Nobody has said anything snide, or put anybody down. Your post is typical of those lacking a sense of humour, and who think being professional is synonymous with being formal. It is not. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Godfrey Guerzon Sent: 20 March 2008 14:17 To: histoinfo@comcast.net; Gudrun Lang Cc: histonet@lists.utsouthwestern.edu Subject: Re: AW: [Histonet] Kidney Bx question We are supposed to be professionals. What we post are real issues in our situation and ask for help. Professionals don't make snide remarks and put down our colleagues. If we think that it will raise our stature as professionals when we make a joke and condescending comments on things that we need help on, we are greatly mistaken. A wise man once said "if you have nothing good to say about anything - shut up". We need to understand that each situation is different - who are we to judge? We post a real issue in our own situation and we don't need "smart" colleagues make a joke of our situation. Do we want to be treated as professionals - then let us communicate as professionals. No snide remarks or condescending comments - PLEASE!!! - Help and good ideas are welcome - if you have nothiing to contribute to the issue at hand - please don't waste your (and ours) valuable time with useless comments. Godfrey >>> "Gudrun Lang" 3/20/2008 9:53 AM >>> Would you be so kind and explain, why you have to do a short run. Are these indeed NTX-biopsies, that need a report as fast as possible? Or is it just the fear to over-process the biopsies? We have ntx from time to time, but even then, the clinicians wait for the report until the next day, noon. So we can process the biopsies with our regular histo and do the specialstains (Jones, PAS, Congored, EvG, SFOG = kind of trichrome), immunohistochemistry and immunofluorescence. We process the needlebiopsies in sponges and with the regular 13-hour protocol. Regarding IHC a fixation-time under 6 hours could also drive to problems. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von histoinfo@comcast.net Gesendet: Donnerstag, 20. M?rz 2008 14:01 An: Rene J Buesa; Marshall Terry Dr,Consultant Histopathologist; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] Kidney Bx question Rene, Yes they are agitated. And the tearing problem that I originally described is fairly infrequent. It's just that when it does happen we can't figure out why. When it does happen nothing that we can see is any different than any other day. Nothing in common as far as collection site either. I am beginning to think more and more that it may be on days that we have 5 or 6 bxs that are all run together. But even then it is not every bx. I think we will take better steps to prevent solution carryover and see what happens. Of course it is so infrequent that we have this problem that it will be months before we can say that we "fixed" the problem. I would still like to compare times with others out there though. So please share your times for hand processing. Thanks, Jennifer -------------- Original message -------------- From: Rene J Buesa For hand processing at room temperature that schedule is too short, unless the samples are agitated. Ren? J. "Marshall Terry Dr, Consultant Histopathologist" wrote: Do you have a train to catch? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histoinfo@comcast.net Sent: 19 March 2008 17:14 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kidney Bx question We have been having some trouble with the inner surface of the tubules looking torn look to the tissue. We hand process all our kidney bx's with the times as follows. 1-formalin 15 minutes 2-50% ethanol 15 minutes 3-70% ethanol 15 minutes 4-80% ethanol 15 minutes 5-95% ethanol 15 minutes 6-100% ethanol 15 minutes 7-100% ethanol 15 minutes 9-xylene 15 minutes 10-xylene 15 minutes Parraffin x2, 20 minutes each. They are then cut at 1 micron for PAS & JONES and 2 microns for H&E & TRICHROME. We are trying to determine if this tearing is an artifact from processing, collection or some other factor we haven't thought of. Has anyone else come across this or a similar problem. All thoughts and suggestions welcome. Jennifer Saunders HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ###################################################################### This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. ###################################################################### _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------------------------------- THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE ADDRESSEE LISTED ABOVE. This record has been disclosed in accordance with Subtitle 3 of Title 4 of the Health-General Article of the Annotated Code of Maryland. Further disclosure of medical information contained herein is prohibited. If you are neither the intended recipient nor the individual responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure of patient information is strictly prohibited. If you have received this email in error, immediately notify us by telephone or return email. ----------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From: "Horn, Hazel V" Subject: RE: AW: [Histonet] Kidney Bx question CC: histonet@lists.utsouthwestern.edu Date: Thu, 20 Mar 2008 10:25:07 -0500 To: "Godfrey Guerzon" , , "Gudrun Lang" Amen Godfrey I was thinking the same thing..... Jennifer, I would extend the processing times by about 5 minutes each and I would add one more formalin... Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Godfrey Guerzon Sent: Thursday, March 20, 2008 9:17 AM To: histoinfo@comcast.net; Gudrun Lang Cc: histonet@lists.utsouthwestern.edu Subject: Re: AW: [Histonet] Kidney Bx question We are supposed to be professionals. What we post are real issues in our situation and ask for help. Professionals don't make snide remarks and put down our colleagues. If we think that it will raise our stature as professionals when we make a joke and condescending comments on things that we need help on, we are greatly mistaken. A wise man once said "if you have nothing good to say about anything - shut up". We need to understand that each situation is different - who are we to judge? We post a real issue in our own situation and we don't need "smart" colleagues make a joke of our situation. Do we want to be treated as professionals - then let us communicate as professionals. No snide remarks or condescending comments - PLEASE!!! - Help and good ideas are welcome - if you have nothiing to contribute to the issue at hand - please don't waste your (and ours) valuable time with useless comments. Godfrey >>> "Gudrun Lang" 3/20/2008 9:53 AM >>> Would you be so kind and explain, why you have to do a short run. Are these indeed NTX-biopsies, that need a report as fast as possible? Or is it just the fear to over-process the biopsies? We have ntx from time to time, but even then, the clinicians wait for the report until the next day, noon. So we can process the biopsies with our regular histo and do the specialstains (Jones, PAS, Congored, EvG, SFOG = kind of trichrome), immunohistochemistry and immunofluorescence. We process the needlebiopsies in sponges and with the regular 13-hour protocol. Regarding IHC a fixation-time under 6 hours could also drive to problems. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von histoinfo@comcast.net Gesendet: Donnerstag, 20. M?rz 2008 14:01 An: Rene J Buesa; Marshall Terry Dr,Consultant Histopathologist; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] Kidney Bx question Rene, Yes they are agitated. And the tearing problem that I originally described is fairly infrequent. It's just that when it does happen we can't figure out why. When it does happen nothing that we can see is any different than any other day. Nothing in common as far as collection site either. I am beginning to think more and more that it may be on days that we have 5 or 6 bxs that are all run together. But even then it is not every bx. I think we will take better steps to prevent solution carryover and see what happens. Of course it is so infrequent that we have this problem that it will be months before we can say that we "fixed" the problem. I would still like to compare times with others out there though. So please share your times for hand processing. Thanks, Jennifer -------------- Original message -------------- From: Rene J Buesa For hand processing at room temperature that schedule is too short, unless the samples are agitated. Ren? J. "Marshall Terry Dr, Consultant Histopathologist" wrote: Do you have a train to catch? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histoinfo@comcast.net Sent: 19 March 2008 17:14 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kidney Bx question We have been having some trouble with the inner surface of the tubules looking torn look to the tissue. We hand process all our kidney bx's with the times as follows. 1-formalin 15 minutes 2-50% ethanol 15 minutes 3-70% ethanol 15 minutes 4-80% ethanol 15 minutes 5-95% ethanol 15 minutes 6-100% ethanol 15 minutes 7-100% ethanol 15 minutes 9-xylene 15 minutes 10-xylene 15 minutes Parraffin x2, 20 minutes each. They are then cut at 1 micron for PAS & JONES and 2 microns for H&E & TRICHROME. We are trying to determine if this tearing is an artifact from processing, collection or some other factor we haven't thought of. Has anyone else come across this or a similar problem. All thoughts and suggestions welcome. Jennifer Saunders HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ###################################################################### This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. ###################################################################### _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------------------------------- THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE ADDRESSEE LISTED ABOVE. This record has been disclosed in accordance with Subtitle 3 of Title 4 of the Health-General Article of the Annotated Code of Maryland. Further disclosure of medical information contained herein is prohibited. If you are neither the intended recipient nor the individual responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure of patient information is strictly prohibited. If you have received this email in error, immediately notify us by telephone or return email. ----------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From b-frederick <@t> northwestern.edu Thu Mar 20 15:05:07 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Mar 20 15:05:12 2008 Subject: [Histonet] CLIA In-Reply-To: <785069.1345.qm@web63411.mail.re1.yahoo.com> Message-ID: <000601c88ac5$b592b420$d00f7ca5@lurie.northwestern.edu> Well if you were in Houston, one could open the door and get the humidity!!!! Not that Chicago is dry in the summer either. I imagine our waterbaths help contribute to the humidity. Bernice. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matt Bancroft Sent: Thursday, March 20, 2008 12:25 PM To: Blazek, Linda; Morken, Tim; mickie25@netzero.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CLIA Becareful, they will ding on it. I think that it is crazy. I am lucky and eveything was in specks but she was hunting for something "Blazek, Linda" wrote: After the first email about temps and humidity I checked all my operator manuals and found that if we are below 5 or over 40 C and over 60% humidity we would be out of range. I know I'm not working in that environment! Linda -----Original Message----- From: Morken, Tim [mailto:tim.morken@thermofisher.com] Sent: Thursday, March 20, 2008 12:10 PM To: mickie25@netzero.net; Matt Bancroft; Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CLIA Adding the ranges certainly adds to the tidbits of information that could be used for troubleshooting., but it is humorous in that if the lab environment was outiside the temperature and humidity ranges given for most equipment, no one would be working in the lab and the equipment would most likely be off due the power failure that caused the temperature and humidity to be outside the ranges! Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mickie Johnson Sent: Thursday, March 20, 2008 7:07 AM To: 'Matt Bancroft'; 'Blazek, Linda'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CLIA Matt, I think this is interesting because I am hearing more and more that CLIA inspectors are requiring these ranges on the chart. Is anyone else having CLIA inspectors ding them about operational humidity and temperature ranges on temperature charts? Some CLIA inspectors require room temperatures to be recorded as well. Everyone's input would be appreciated. Thanks. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matt Bancroft Sent: Thursday, March 20, 2008 5:04 AM To: Blazek, Linda; histonet@lists.utsouthwestern.edu Subject: [Histonet] CLIA For those of you that have to go thru a CLIA inspeciton, I had mine yesterday and came across a issue, that in my 15 years of histology have never came across so I thought that I would share with you all. The inspector that I had opened up the owners manuals of my equipment (paraffin pot, water-bath, embedding center, etc) and looked at the manufactors recommendations for room temp and room humidity. She was going to ding me on not haveing those ranges on my QC chats, but I changed them while she was here. So if you do not have those ranges on your QC charts I would recommened it. Matt Bancroft Laboratory Manager --------------------------------- Never miss a thing. Make Yahoo your homepage. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tracy.bergeron <@t> biogenidec.com Thu Mar 20 15:23:28 2008 From: tracy.bergeron <@t> biogenidec.com (Tracy Bergeron) Date: Thu Mar 20 15:23:38 2008 Subject: [Histonet] Tracy Bergeron is out of the office. Message-ID: I will be out of the office starting 03/20/2008 and will not return until 03/25/2008. I will respond to your message when I return. From alaskagirl1950 <@t> yahoo.com Thu Mar 20 15:37:57 2008 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Thu Mar 20 15:38:00 2008 Subject: AW: [Histonet] Kidney Bx question In-Reply-To: <90354A475B420441B2A0396E5008D4965E207E@copc-sbs.COPC.local> Message-ID: <336075.45728.qm@web52501.mail.re2.yahoo.com> Thomas, Very well said! I enjoy the humor and enjoy laughing at myself whenever possible. (Which seems to be a great deal, not sure what that means.) I think we all sometimes wear our feeling a little too close to our cuffs. I know when I did I was always hurt about something. Maybe I am now so old it doesn't matter anymore. Gee, now I'm depressed ;(. Patricia --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From pcprabuin <@t> gmail.com Thu Mar 20 23:31:40 2008 From: pcprabuin <@t> gmail.com (prabu prabu) Date: Thu Mar 20 23:31:45 2008 Subject: [Histonet] Re: Histonet Digest, Vol 52, Issue 27 In-Reply-To: References: <20080318170213.4742B3B3072@smhex1.shorememorial.org> Message-ID: <1c7fdf750803202131s260f3f31uafec80fce64238c0@mail.gmail.com> Dear all, Anybody using Leica ST 4040 linear stainer for H & E staining ?. Please share your staining procedure. I am not getting proper eosin staining. On Tue, Mar 18, 2008 at 10:19 AM, wrote: > We use the CPT codes 88173 88172 for FNA's > > > Andrea J Weiss BST CT (ASCP) > Cytotechnologist > 609 653 3577 Ext 4907 > aweiss@shorememorial.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jnocito <@t> satx.rr.com Fri Mar 21 00:39:27 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Mar 21 00:39:06 2008 Subject: [Histonet] CLIA References: <1F937FB30BDB7C4A9F39F83FEA8D379F9F532C@bruexchange1.digestivespecialists.com><320362.54247.qm@web63401.mail.re1.yahoo.com> Message-ID: <002101c88b15$edc67700$0302a8c0@yourxhtr8hvc4p> The CAP General checklist has a question about are room temps and humidity recorded, but nothing about ranges. Here's a question for you. What do you do if your HVAC system goes out and your room temp is 85 degrees? Does your processors and embedding machines stop working? Being a CAP inspector myself, I hate hearing stuff like this. It just fries my toes. JTT ----- Original Message ----- From: "Mickie Johnson" To: "'Matt Bancroft'" ; "'Blazek, Linda'" ; Sent: Thursday, March 20, 2008 9:07 AM Subject: RE: [Histonet] CLIA > Matt, > > I think this is interesting because I am hearing more and more that CLIA > inspectors are requiring these ranges on the chart. Is anyone else having > CLIA inspectors ding them about operational humidity and temperature > ranges > on temperature charts? Some CLIA inspectors require room temperatures to > be > recorded as well. Everyone's input would be appreciated. Thanks. > > Mickie > > Mickie Johnson, B.S., HTL(ASCP) > Mohs Histology Consulting Services, LLC > & Mohs Lab Staffing > 2507 S. Manito Blvd. > Spokane, WA 99203 > 509-954-7134 > FAX 509-624-3926 > Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com > Email: mickie25@netzero.net > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matt > Bancroft > Sent: Thursday, March 20, 2008 5:04 AM > To: Blazek, Linda; histonet@lists.utsouthwestern.edu > Subject: [Histonet] CLIA > > > > For those of you that have to go thru a CLIA inspeciton, I had mine > yesterday and came across a issue, that in my 15 years of histology have > never came across so I thought that I would share with you all. The > inspector that I had opened up the owners manuals of my equipment > (paraffin > pot, water-bath, embedding center, etc) and looked at the manufactors > recommendations for room temp and room humidity. She was going to ding me > on not haveing those ranges on my QC chats, but I changed them while she > was > here. So if you do not have those ranges on your QC charts I would > recommened it. > > Matt Bancroft > Laboratory Manager > > > --------------------------------- > Never miss a thing. Make Yahoo your homepage. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo <@t> f2s.com Fri Mar 21 04:03:25 2008 From: kemlo <@t> f2s.com (kemlo) Date: Fri Mar 21 04:04:58 2008 Subject: AW: [Histonet] Kidney Bx question In-Reply-To: <90354A475B420441B2A0396E5008D4965E207E@copc-sbs.COPC.local> References: <5C0BED61F529364E86309CADEA63FEF20163F3A5@TRFT-EX01.xRothGen.nhs.uk> <90354A475B420441B2A0396E5008D4965E207E@copc-sbs.COPC.local> Message-ID: <6A08DFA66CF541449A5064B5F3E1EAEA@KemloPC> If you are ever in Weston Super Mare then make for the boatyard in Uphill; there's a small Hospital just before the boatyard less than a mile from the beach. Come to reception ask for Kemlo, General Manager, they'll get me and we can go for a pint. Look up Weston Super Mare in Google!!! Sea goes out to Wales (whales), beach is composed of a bit of sand and Estuary mud, there are donkeys on the beach and the Welsh try and walk home get stuck in the mud and drown; there's a tractor in there somewhere!!! You can have a beer or a cider if you want; North Somerset specialises in paint stripper called cider at about 50p a pint (fraid that's a dollar for a little more than a US pint). Or I could come to Bend Oregon, but I bet the beer's cold and fizzy and you don't have cider!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: 20 March 2008 17:12 To: Marshall Terry Dr, Consultant Histopathologist Cc: histonet@lists.utsouthwestern.edu Subject: RE: AW: [Histonet] Kidney Bx question Godfrey, Terry, Kemlo et al, I believe it was Eleanor Roosevelt who said, "No one can make you feel bad (unhappy, inferior, insert whatever negative word you like here) unless you allow them to!" I may not have the quote down exactly as stated but that is the gist of it. And unfortunately the world runs on perceptions. People are going to take things in whatever way they are perceived. This of course leads to misunderstandings, ruffled feathers etc. So, I must say Godfrey, I've been watching and posting the histonet for some time. I have followed many a thread involving Terry and Kemlo. They are both great resources, observant, knowledgable and yes...most of the time doing their best to inject levity whenever possible. And some folks don't get on with that. Not yet, having had the pleasure of personally meeting either of them, I think I can safely say that in no way would either of them have meant a comment to be an insult of any degree. I believe many people use humor as a way of looking at problems and then trying to determine solutions. Sometimes it is necessary to point something obvious (in this case TAT vs. quality) for the sake of bean counters, upper administrators or any other party that influences our work lives and yet doesn't fully understand where we're coming from. I am truly sorry if you feel this whole thing was insulting. I assure you I would not have. And please understand I'm not trying to apologize for anyone here, I'm just interested in fostering better understanding. Have a great day! Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr, Consultant Histopathologist Sent: Thursday, March 20, 2008 7:32 AM To: Godfrey Guerzon; histoinfo@comcast.net; Gudrun Lang Cc: histonet@lists.utsouthwestern.edu Subject: RE: AW: [Histonet] Kidney Bx question Godfrey, Nobody has said anything snide, or put anybody down. Your post is typical of those lacking a sense of humour, and who think being professional is synonymous with being formal. It is not. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Godfrey Guerzon Sent: 20 March 2008 14:17 To: histoinfo@comcast.net; Gudrun Lang Cc: histonet@lists.utsouthwestern.edu Subject: Re: AW: [Histonet] Kidney Bx question We are supposed to be professionals. What we post are real issues in our situation and ask for help. Professionals don't make snide remarks and put down our colleagues. If we think that it will raise our stature as professionals when we make a joke and condescending comments on things that we need help on, we are greatly mistaken. A wise man once said "if you have nothing good to say about anything - shut up". We need to understand that each situation is different - who are we to judge? We post a real issue in our own situation and we don't need "smart" colleagues make a joke of our situation. Do we want to be treated as professionals - then let us communicate as professionals. No snide remarks or condescending comments - PLEASE!!! - Help and good ideas are welcome - if you have nothiing to contribute to the issue at hand - please don't waste your (and ours) valuable time with useless comments. Godfrey >>> "Gudrun Lang" 3/20/2008 9:53 AM >>> Would you be so kind and explain, why you have to do a short run. Are these indeed NTX-biopsies, that need a report as fast as possible? Or is it just the fear to over-process the biopsies? We have ntx from time to time, but even then, the clinicians wait for the report until the next day, noon. So we can process the biopsies with our regular histo and do the specialstains (Jones, PAS, Congored, EvG, SFOG = kind of trichrome), immunohistochemistry and immunofluorescence. We process the needlebiopsies in sponges and with the regular 13-hour protocol. Regarding IHC a fixation-time under 6 hours could also drive to problems. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von histoinfo@comcast.net Gesendet: Donnerstag, 20. M?rz 2008 14:01 An: Rene J Buesa; Marshall Terry Dr,Consultant Histopathologist; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] Kidney Bx question Rene, Yes they are agitated. And the tearing problem that I originally described is fairly infrequent. It's just that when it does happen we can't figure out why. When it does happen nothing that we can see is any different than any other day. Nothing in common as far as collection site either. I am beginning to think more and more that it may be on days that we have 5 or 6 bxs that are all run together. But even then it is not every bx. I think we will take better steps to prevent solution carryover and see what happens. Of course it is so infrequent that we have this problem that it will be months before we can say that we "fixed" the problem. I would still like to compare times with others out there though. So please share your times for hand processing. Thanks, Jennifer -------------- Original message -------------- From: Rene J Buesa For hand processing at room temperature that schedule is too short, unless the samples are agitated. Ren? J. "Marshall Terry Dr, Consultant Histopathologist" wrote: Do you have a train to catch? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histoinfo@comcast.net Sent: 19 March 2008 17:14 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kidney Bx question We have been having some trouble with the inner surface of the tubules looking torn look to the tissue. We hand process all our kidney bx's with the times as follows. 1-formalin 15 minutes 2-50% ethanol 15 minutes 3-70% ethanol 15 minutes 4-80% ethanol 15 minutes 5-95% ethanol 15 minutes 6-100% ethanol 15 minutes 7-100% ethanol 15 minutes 9-xylene 15 minutes 10-xylene 15 minutes Parraffin x2, 20 minutes each. They are then cut at 1 micron for PAS & JONES and 2 microns for H&E & TRICHROME. We are trying to determine if this tearing is an artifact from processing, collection or some other factor we haven't thought of. Has anyone else come across this or a similar problem. All thoughts and suggestions welcome. Jennifer Saunders HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ###################################################################### This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. ###################################################################### _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------------------------------- THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE ADDRESSEE LISTED ABOVE. This record has been disclosed in accordance with Subtitle 3 of Title 4 of the Health-General Article of the Annotated Code of Maryland. Further disclosure of medical information contained herein is prohibited. If you are neither the intended recipient nor the individual responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure of patient information is strictly prohibited. If you have received this email in error, immediately notify us by telephone or return email. ----------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Fri Mar 21 08:08:50 2008 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Fri Mar 21 08:08:58 2008 Subject: AW: [Histonet] Kidney Bx question In-Reply-To: <979FF5962E234F45B06CF0DB7C1AABB21617F470@chi2k3ms01.columbuschildrens.net> Message-ID: Here, here Ronnie!!! Hope to here from you in a couple of months. Glen Dawson Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Houston, Ronald Sent: Thursday, March 20, 2008 10:10 AM To: Marshall Terry Dr,Consultant Histopathologist; Godfrey Guerzon; histoinfo@comcast.net; Gudrun Lang Cc: histonet@lists.utsouthwestern.edu Subject: RE: AW: [Histonet] Kidney Bx question Importance: High And we wonder why people get so p*****d off with Histonet and unsubscribe for long periods of time................. Get a grip people! Take a pill and calm down! Terry & Kemlo, it was brought up previously about the difference in humor on both sides on the Atlantic. Even though many parts of the country are well exposed to British TV, especially British comedies, much of the humor is lost on them. There is also a great difference in demands placed on histology labs & personnel in the US. The turn-around-times that exist in the UK at present, just aren't tolerated over here. Is everything a bowl of cherries over here? Absolutely not, but staff have to work under the expectations and constraints that are placed/forced on them. To all the Americans that get involved and respond with outbursts - lighten up! Life is too short to send your blood pressure up 20 points over something as trivial as this. I feel competent to qualify these statements because I worked in Scotland for 18 years, 4 in Middle East, and 16 years in the US. Well I've said my piece; I'm signing off for another couple of months till peoples egos subside and we can get back to the purpose Histonet was established. Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 Ronald.Houston@NationwideChildrens.org Columbus Children's Hospital is now Nationwide Children's Hospital www.NationwideChildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Thursday, March 20, 2008 10:32 AM To: Godfrey Guerzon; histoinfo@comcast.net; Gudrun Lang Cc: histonet@lists.utsouthwestern.edu Subject: RE: AW: [Histonet] Kidney Bx question Godfrey, Nobody has said anything snide, or put anybody down. Your post is typical of those lacking a sense of humour, and who think being professional is synonymous with being formal. It is not. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Godfrey Guerzon Sent: 20 March 2008 14:17 To: histoinfo@comcast.net; Gudrun Lang Cc: histonet@lists.utsouthwestern.edu Subject: Re: AW: [Histonet] Kidney Bx question We are supposed to be professionals. What we post are real issues in our situation and ask for help. Professionals don't make snide remarks and put down our colleagues. If we think that it will raise our stature as professionals when we make a joke and condescending comments on things that we need help on, we are greatly mistaken. A wise man once said "if you have nothing good to say about anything - shut up". We need to understand that each situation is different - who are we to judge? We post a real issue in our own situation and we don't need "smart" colleagues make a joke of our situation. Do we want to be treated as professionals - then let us communicate as professionals. No snide remarks or condescending comments - PLEASE!!! - Help and good ideas are welcome - if you have nothiing to contribute to the issue at hand - please don't waste your (and ours) valuable time with useless comments. Godfrey >>> "Gudrun Lang" 3/20/2008 9:53 AM >>> Would you be so kind and explain, why you have to do a short run. Are these indeed NTX-biopsies, that need a report as fast as possible? Or is it just the fear to over-process the biopsies? We have ntx from time to time, but even then, the clinicians wait for the report until the next day, noon. So we can process the biopsies with our regular histo and do the specialstains (Jones, PAS, Congored, EvG, SFOG = kind of trichrome), immunohistochemistry and immunofluorescence. We process the needlebiopsies in sponges and with the regular 13-hour protocol. Regarding IHC a fixation-time under 6 hours could also drive to problems. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von histoinfo@comcast.net Gesendet: Donnerstag, 20. M?rz 2008 14:01 An: Rene J Buesa; Marshall Terry Dr,Consultant Histopathologist; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] Kidney Bx question Rene, Yes they are agitated. And the tearing problem that I originally described is fairly infrequent. It's just that when it does happen we can't figure out why. When it does happen nothing that we can see is any different than any other day. Nothing in common as far as collection site either. I am beginning to think more and more that it may be on days that we have 5 or 6 bxs that are all run together. But even then it is not every bx. I think we will take better steps to prevent solution carryover and see what happens. Of course it is so infrequent that we have this problem that it will be months before we can say that we "fixed" the problem. I would still like to compare times with others out there though. So please share your times for hand processing. Thanks, Jennifer -------------- Original message -------------- From: Rene J Buesa For hand processing at room temperature that schedule is too short, unless the samples are agitated. Ren? J. "Marshall Terry Dr, Consultant Histopathologist" wrote: Do you have a train to catch? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histoinfo@comcast.net Sent: 19 March 2008 17:14 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kidney Bx question We have been having some trouble with the inner surface of the tubules looking torn look to the tissue. We hand process all our kidney bx's with the times as follows. 1-formalin 15 minutes 2-50% ethanol 15 minutes 3-70% ethanol 15 minutes 4-80% ethanol 15 minutes 5-95% ethanol 15 minutes 6-100% ethanol 15 minutes 7-100% ethanol 15 minutes 9-xylene 15 minutes 10-xylene 15 minutes Parraffin x2, 20 minutes each. They are then cut at 1 micron for PAS & JONES and 2 microns for H&E & TRICHROME. We are trying to determine if this tearing is an artifact from processing, collection or some other factor we haven't thought of. Has anyone else come across this or a similar problem. All thoughts and suggestions welcome. Jennifer Saunders HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ###################################################################### This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. If you are not the intended recipient please accept our apologies. 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Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------------------------------- THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE ADDRESSEE LISTED ABOVE. This record has been disclosed in accordance with Subtitle 3 of Title 4 of the Health-General Article of the Annotated Code of Maryland. Further disclosure of medical information contained herein is prohibited. If you are neither the intended recipient nor the individual responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure of patient information is strictly prohibited. If you have received this email in error, immediately notify us by telephone or return email. ----------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mickie25 <@t> netzero.net Fri Mar 21 08:24:37 2008 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Fri Mar 21 08:25:24 2008 Subject: [Histonet] Re: Inspectors/ CAP JCAHO CLIA In-Reply-To: <47E2B506.3090100@bms.com> References: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F549@lmhsmail.lmhealth.org> <47E2B506.3090100@bms.com> Message-ID: I agree! What has room humidity got to do with anything in histology including Mohs. It might affect peoples sensibilities when the inspector arrives and hassles them about humidity! I remember a CAP inspector berate our microbiology supervisor at a summation meeting because he found one temperature log with a missing day out of a whole year! We all knew our microbiology department was one of the best in the state. The state had recently designated them as the primary TB testing lab for the whole state! Too bad there is not some review of requirements for thing like certification of timers. Thanks for listening to my 2 cents worth! Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth M Heimrich Sent: Thursday, March 20, 2008 12:04 PM To: Undisclosed-recipients: Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Inspectors/ CAP JCAHO CLIA This whole thread really makes me wonder...does anyone have common sense anymore? Are these 'inspectors' just looking to ding the labs so that they look better to their superiors? Where is it going to stop? Next they'll want the temp ranges of the refrigerators each time the door is opened. What does the air temp and humidity really have to do with the quality of the service being provided? Do these minscule details make one lab better than another? I think not. You can become so caught up in these inspections that you make more mistakes regarding the patients' details. I think they are missing the point! It is just another check off on the list for the paper pushers. I am really glad I do not work in a hospital/ clinical setting. To all my fellow histonetters in these settings; my hat is off to you, for being able to perform your duties well, and deal with all the BS surrounding inspections you deserve a lot more than what the average salary is. I feel for you all!!! Beth Tom McNemar wrote: >I had a JCAHO inspector tell me about 6 years ago that I should have the temperature ranges on the charts but have never had anyone suggest the operational humidity... > >Tom McNemar, HT(ASCP) >Histology Co-ordinator >Licking Memorial Health Systems >(740) 348-4163 >(740) 348-4166 >tmcnemar@lmhealth.org >www.LMHealth.org > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of >AWeiss@shorememorial.org >Sent: Thursday, March 20, 2008 1:09 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Re: Histonet Digest, Vol 52, Issue 30 > > >Matt, > >I think this is interesting because I am hearing more and more that CLIA >inspectors are requiring these ranges on the chart. Is anyone else having >CLIA inspectors ding them about operational humidity and temperature ranges >on temperature charts? Some CLIA inspectors require room temperatures to be >recorded as well. Everyone's input would be appreciated. Thanks. > >Mickie > >Mickie Johnson, B.S., HTL(ASCP) >Mohs Histology Consulting Services, LLC > & Mohs Lab Staffing >2507 S. Manito Blvd. >Spokane, WA 99203 >509-954-7134 >FAX 509-624-3926 > > >We do this monitoring for flow cytometry, there is a question for temp and >humidy for CAP so we record this daily, with a monitor that records the >temp 3x's./ day and the humidity. You can set the monitoring times for any >interval you need. Then I download this monthly as part of the qc. > > >Andrea J Weiss BST CT (ASCP) >Cytotechnologist >609 653 3577 Ext 4907 >aweiss@shorememorial.org > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Jan.Minshew <@t> leica-microsystems.com Fri Mar 21 10:29:50 2008 From: Jan.Minshew <@t> leica-microsystems.com (Jan.Minshew@leica-microsystems.com) Date: Fri Mar 21 10:30:02 2008 Subject: [Histonet] Re: Inspectors/ CAP JCAHO CLIA In-Reply-To: Message-ID: Hello, I've been following this thread for a few days and I thought I would inject a bit of information from an instrument perspective. Room temperature and humidity can be extremely important factors when operating and maintaining equipment, especially if the equipment is used at temperatures that are higher or lower than typical air conditioned room temperatures (remember that many laboratories in other countries and a few in the U.S. don't enjoy the luxury of air conditioning). Most instruments have electronic components, lubricants and other materials that have temperature and/or humidity tolerances. And, if you have ever used a refrigerated instrument like a cryostat or low temperature freezer in a high humidity area, you will know how quickly they develop frost, which might necessitate more frequent or lengthy defrost cycles. These environmental operating considerations are very important in order to keep instrumentation operating correctly throughout its expected life cycle. I understand your frustration with all of the regulatory agencies that demand your compliance (and I'm not defending them). I'm just trying to help with the possible logic behind this particular requirement. Best wishes, Jan Minshew, HT/HTL(ASCP) Marketing Manager Leica Microsystems Biosystems Division 2345 Waukegan Road Bannockburn, IL 60015 800.248.0123 Toll Free 847.405.7051 Direct 847.405.6560 Fax www.leica-microsystems.com Click Here for this month's special offers! "Mickie Johnson" To Sent by: "'Elizabeth M Heimrich'" histonet-bounces@ , lists.utsouthwest "'Undisclosed-recipients:'" ern.edu cc histonet@lists.utsouthwestern.edu Subject 03/21/2008 08:24 RE: [Histonet] Re: Inspectors/ CAP AM JCAHO CLIA Please respond to mickie25@netzero. net I agree! What has room humidity got to do with anything in histology including Mohs. It might affect peoples sensibilities when the inspector arrives and hassles them about humidity! I remember a CAP inspector berate our microbiology supervisor at a summation meeting because he found one temperature log with a missing day out of a whole year! We all knew our microbiology department was one of the best in the state. The state had recently designated them as the primary TB testing lab for the whole state! Too bad there is not some review of requirements for thing like certification of timers. Thanks for listening to my 2 cents worth! Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth M Heimrich Sent: Thursday, March 20, 2008 12:04 PM To: Undisclosed-recipients: Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Inspectors/ CAP JCAHO CLIA This whole thread really makes me wonder...does anyone have common sense anymore? Are these 'inspectors' just looking to ding the labs so that they look better to their superiors? Where is it going to stop? Next they'll want the temp ranges of the refrigerators each time the door is opened. What does the air temp and humidity really have to do with the quality of the service being provided? Do these minscule details make one lab better than another? I think not. You can become so caught up in these inspections that you make more mistakes regarding the patients' details. I think they are missing the point! It is just another check off on the list for the paper pushers. I am really glad I do not work in a hospital/ clinical setting. To all my fellow histonetters in these settings; my hat is off to you, for being able to perform your duties well, and deal with all the BS surrounding inspections you deserve a lot more than what the average salary is. I feel for you all!!! Beth Tom McNemar wrote: >I had a JCAHO inspector tell me about 6 years ago that I should have the temperature ranges on the charts but have never had anyone suggest the operational humidity... > >Tom McNemar, HT(ASCP) >Histology Co-ordinator >Licking Memorial Health Systems >(740) 348-4163 >(740) 348-4166 >tmcnemar@lmhealth.org >www.LMHealth.org > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of >AWeiss@shorememorial.org >Sent: Thursday, March 20, 2008 1:09 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Re: Histonet Digest, Vol 52, Issue 30 > > >Matt, > >I think this is interesting because I am hearing more and more that CLIA >inspectors are requiring these ranges on the chart. Is anyone else having >CLIA inspectors ding them about operational humidity and temperature ranges >on temperature charts? Some CLIA inspectors require room temperatures to be >recorded as well. Everyone's input would be appreciated. Thanks. > >Mickie > >Mickie Johnson, B.S., HTL(ASCP) >Mohs Histology Consulting Services, LLC > & Mohs Lab Staffing >2507 S. Manito Blvd. >Spokane, WA 99203 >509-954-7134 >FAX 509-624-3926 > > >We do this monitoring for flow cytometry, there is a question for temp and >humidy for CAP so we record this daily, with a monitor that records the >temp 3x's./ day and the humidity. You can set the monitoring times for any >interval you need. Then I download this monthly as part of the qc. > > >Andrea J Weiss BST CT (ASCP) >Cytotechnologist >609 653 3577 Ext 4907 >aweiss@shorememorial.org > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From gu.lang <@t> gmx.at Fri Mar 21 13:09:17 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Mar 21 13:09:26 2008 Subject: AW: [Histonet] Re: Inspectors/ CAP JCAHO CLIA In-Reply-To: Message-ID: <000901c88b7e$ae3ca7d0$eeeea8c0@dielangs.at> In this country not only the instruments are in the focus of interest but also the employers welfare. The working environment should be in a healthy range. (.. but this isn't tested daily) There are laws and regulations for the working place. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Mickie Johnson Gesendet: Freitag, 21. M?rz 2008 14:25 An: 'Elizabeth M Heimrich'; 'Undisclosed-recipients:' Cc: histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] Re: Inspectors/ CAP JCAHO CLIA I agree! What has room humidity got to do with anything in histology including Mohs. It might affect peoples sensibilities when the inspector arrives and hassles them about humidity! I remember a CAP inspector berate our microbiology supervisor at a summation meeting because he found one temperature log with a missing day out of a whole year! We all knew our microbiology department was one of the best in the state. The state had recently designated them as the primary TB testing lab for the whole state! Too bad there is not some review of requirements for thing like certification of timers. Thanks for listening to my 2 cents worth! Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth M Heimrich Sent: Thursday, March 20, 2008 12:04 PM To: Undisclosed-recipients: Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Inspectors/ CAP JCAHO CLIA This whole thread really makes me wonder...does anyone have common sense anymore? Are these 'inspectors' just looking to ding the labs so that they look better to their superiors? Where is it going to stop? Next they'll want the temp ranges of the refrigerators each time the door is opened. What does the air temp and humidity really have to do with the quality of the service being provided? Do these minscule details make one lab better than another? I think not. You can become so caught up in these inspections that you make more mistakes regarding the patients' details. I think they are missing the point! It is just another check off on the list for the paper pushers. I am really glad I do not work in a hospital/ clinical setting. To all my fellow histonetters in these settings; my hat is off to you, for being able to perform your duties well, and deal with all the BS surrounding inspections you deserve a lot more than what the average salary is. I feel for you all!!! Beth Tom McNemar wrote: >I had a JCAHO inspector tell me about 6 years ago that I should have the temperature ranges on the charts but have never had anyone suggest the operational humidity... > >Tom McNemar, HT(ASCP) >Histology Co-ordinator >Licking Memorial Health Systems >(740) 348-4163 >(740) 348-4166 >tmcnemar@lmhealth.org >www.LMHealth.org > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of >AWeiss@shorememorial.org >Sent: Thursday, March 20, 2008 1:09 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Re: Histonet Digest, Vol 52, Issue 30 > > >Matt, > >I think this is interesting because I am hearing more and more that CLIA >inspectors are requiring these ranges on the chart. Is anyone else having >CLIA inspectors ding them about operational humidity and temperature ranges >on temperature charts? Some CLIA inspectors require room temperatures to be >recorded as well. Everyone's input would be appreciated. Thanks. > >Mickie > >Mickie Johnson, B.S., HTL(ASCP) >Mohs Histology Consulting Services, LLC > & Mohs Lab Staffing >2507 S. Manito Blvd. >Spokane, WA 99203 >509-954-7134 >FAX 509-624-3926 > > >We do this monitoring for flow cytometry, there is a question for temp and >humidy for CAP so we record this daily, with a monitor that records the >temp 3x's./ day and the humidity. You can set the monitoring times for any >interval you need. Then I download this monthly as part of the qc. > > >Andrea J Weiss BST CT (ASCP) >Cytotechnologist >609 653 3577 Ext 4907 >aweiss@shorememorial.org > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bwhitaker <@t> brownpathology.com Fri Mar 21 13:17:19 2008 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Fri Mar 21 13:12:24 2008 Subject: [Histonet] CLIA In-Reply-To: <002101c88b15$edc67700$0302a8c0@yourxhtr8hvc4p> References: <1F937FB30BDB7C4A9F39F83FEA8D379F9F532C@bruexchange1.digestivespecialists.com><320362.54247.qm@web63401.mail.re1.yahoo.com> <002101c88b15$edc67700$0302a8c0@yourxhtr8hvc4p> Message-ID: <009b01c88b7f$cceada70$66c08f50$@com> Hi All I'll jump in for a Friday flaming.... anything that we are required to record the temperature (or humidity) for, in my opinion, SHOULD have a range that is acceptable, and an action plan for out-of-range. For example, in my warehouse-style storage area, the action plan is if the temperature exceeds 100F in the summer, we are to open the doors and blow some of the lab and office AC (with fans) into the warehouse so our blocks don't start getting too sticky and clump together. We do that until the temperature is back into the acceptable range. Bonnie P. Whitaker Brown & Associates Medical Laboratories bwhitaker@brownpathology.com 713.741.6677 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, March 21, 2008 12:39 AM To: mickie25@netzero.net; 'Matt Bancroft'; 'Blazek, Linda'; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] CLIA The CAP General checklist has a question about are room temps and humidity recorded, but nothing about ranges. Here's a question for you. What do you do if your HVAC system goes out and your room temp is 85 degrees? Does your processors and embedding machines stop working? Being a CAP inspector myself, I hate hearing stuff like this. It just fries my toes. JTT ----- Original Message ----- From: "Mickie Johnson" To: "'Matt Bancroft'" ; "'Blazek, Linda'" ; Sent: Thursday, March 20, 2008 9:07 AM Subject: RE: [Histonet] CLIA > Matt, > > I think this is interesting because I am hearing more and more that CLIA > inspectors are requiring these ranges on the chart. Is anyone else having > CLIA inspectors ding them about operational humidity and temperature > ranges > on temperature charts? Some CLIA inspectors require room temperatures to > be > recorded as well. Everyone's input would be appreciated. Thanks. > > Mickie > > Mickie Johnson, B.S., HTL(ASCP) > Mohs Histology Consulting Services, LLC > & Mohs Lab Staffing > 2507 S. Manito Blvd. > Spokane, WA 99203 > 509-954-7134 > FAX 509-624-3926 > Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com > Email: mickie25@netzero.net > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matt > Bancroft > Sent: Thursday, March 20, 2008 5:04 AM > To: Blazek, Linda; histonet@lists.utsouthwestern.edu > Subject: [Histonet] CLIA > > > > For those of you that have to go thru a CLIA inspeciton, I had mine > yesterday and came across a issue, that in my 15 years of histology have > never came across so I thought that I would share with you all. The > inspector that I had opened up the owners manuals of my equipment > (paraffin > pot, water-bath, embedding center, etc) and looked at the manufactors > recommendations for room temp and room humidity. She was going to ding me > on not haveing those ranges on my QC chats, but I changed them while she > was > here. So if you do not have those ranges on your QC charts I would > recommened it. > > Matt Bancroft > Laboratory Manager > > > --------------------------------- > Never miss a thing. Make Yahoo your homepage. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mulpiece <@t> yahoo.com Fri Mar 21 13:16:46 2008 From: mulpiece <@t> yahoo.com (Michael Muller) Date: Fri Mar 21 13:16:53 2008 Subject: [Histonet] p57 vendor Message-ID: <165388.30537.qm@web54110.mail.re2.yahoo.com> Does anyone have a vendor for p57 that is not RUO? Michael Muller HT-ASCP Converge Dx Peabody, MA. ____________________________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ From suneetimane <@t> hotmail.com Fri Mar 21 13:50:09 2008 From: suneetimane <@t> hotmail.com (Suneeti Mane) Date: Fri Mar 21 13:50:13 2008 Subject: [Histonet] images of blood clot Message-ID: Hello I am looking for some images of blood clots. We are trying to make clots for research purpose and want to see how comparable are our lab-made clots to the real-life clots. sm _________________________________________________________________ Windows Live Hotmail is giving away Zunes. http://www.windowslive-hotmail.com/ZuneADay/?locale=en-US&ocid=TXT_TAGLM_Mobile_Zune_V3 From nfournier <@t> sasktel.net Fri Mar 21 15:32:45 2008 From: nfournier <@t> sasktel.net (N Fournier) Date: Fri Mar 21 15:32:50 2008 Subject: [Histonet] urgent question on double immunofluorescence Message-ID: <5307edaf196a4.47e3c70d@sasktel.net> I have to stain tissue (Fixed rat brain, free-floating) with two different primary antibodies (one is a mouse monoclonal and the other is a goat polyclonal). My secondary fluorescent conjugated antibodies are goat anti-mouse Alexa 488 & donkey anti-goat Alexa 568. I was told that I could stain the tissue by incubating with the antibodies sequentially starting with the goat antibody (for 24 hrs) first followed by its secondary (for 3 hrs) then incubating with the mouse antibody (for 24 hrs) followed by its secondary (for 3 hrs). However, the procedure does not seem to work. I only see staining for the goat antibody and not for the mouse antibody. I know that both antibodies work individually. Does anyone have suggestions? Is it the secondary antibodies that are causing the problems. From anthony <@t> histotechexchange.com Fri Mar 21 20:03:54 2008 From: anthony <@t> histotechexchange.com (anthony@histotechexchange.com) Date: Fri Mar 21 20:23:18 2008 Subject: [Histonet] Histology Atlas In-Reply-To: <47DE935C.8000803@umdnj.edu> References: <47DE536C.C068.0078.0@hsc.wvu.edu> <47DE935C.8000803@umdnj.edu> Message-ID: <2914.65.40.219.161.1206147834.squirrel@host7.wfdns.com> Dear Kimberly: I am a big fan of "Histology A Text and Atlas" by Michael H. Ross and Lynn J. Romrell (ISBN 0-683-07368-0). It is the book I used in university in the UK but it is an American book. Make sure you find the latest edition; the ISBN number is for the second edition. Yours truly, Anthony Williams BSc. HT Histotech Exchange LLC 19 Whitmore St. Lexington, VA 24450 T 1 (302) 383 9780 F 1 (540) 463 3583 anthony@histotechexchange.com I second the choices Jennifer mentioned. We use both of those. > > Geoff > > Kimberly Secrest wrote: >> Can anyone recommend a really good histology atlas. It will be used for >> teaching new students. >> Thanks >> Kim >> >> Kimberly Secrest, HTL, QIHC >> Instructor >> Department of Pathology >> West Virginia University >> 304-293-7628 >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583 > mcauliff@umdnj.edu > ********************************************** > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From laurie <@t> conxis.com Fri Mar 21 20:56:38 2008 From: laurie <@t> conxis.com (Laurie Popp) Date: Fri Mar 21 20:56:10 2008 Subject: [Histonet] Re: Kidney Bx Message-ID: <47E46756.206@conxis.com> we do a fair number of kidney core bx's in our renal lab and they are done on a milestone microwave processor. 90 minute run with formalin/absolute/isopropyl/and paraffin. Laurie Popp HT(ASCP) Mayo Clinic, Rochester, MN From thisisann <@t> aol.com Sat Mar 22 18:19:57 2008 From: thisisann <@t> aol.com (thisisann@aol.com) Date: Sat Mar 22 18:20:13 2008 Subject: [Histonet] Lab Asst. Position/Night Shift Message-ID: <8CA5A8A60E34D39-8A4-4F7E@webmail-nf15.sim.aol.com> I am looking for a Lab Assistant to work from 7:30pm to 4am in the Union County, NJ area.? Job resonsibilities include accessioning and data entering of Pathology specimens.? All interested applicants please fax or e-mail your resume to: Ann Angelo Fax #908-272-1478 e-mail:? AAngelo@QDxpath.com From john_paul1959 <@t> yahoo.com Sun Mar 23 17:19:44 2008 From: john_paul1959 <@t> yahoo.com (John Paul) Date: Sun Mar 23 17:19:48 2008 Subject: [Histonet] copper and PAS Message-ID: <996324.78442.qm@web31305.mail.mud.yahoo.com> I have some tissue in which I can see copper granules clearly on H&E stain but these granules disappear on a PAS stain. Can someone explain this phenomena to me? Thanks John --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From lynnw <@t> nf.sympatico.ca Sun Mar 23 18:31:43 2008 From: lynnw <@t> nf.sympatico.ca (Lynn Wade) Date: Sun Mar 23 18:31:38 2008 Subject: [Histonet] Reprocessing tissue Message-ID: <002401c88d3e$0d922730$0a02a8c0@wadeq8fg62kuom> Hi folks: I am wondering if anyone has had an incident in Patholgy lab where on the tissue processor the reagents got switched inadvertantly. For instance, the 80% alcohol was inadvertantly placed in the last 100% alcohol slot and thus water was reintroduced into the tissue just before xylene, clearing amd paraffin. Has anyone had this occur and how did you recover the tissue? Also, can anyone tell me if there is such a processor that has a system that can be used to log in the cassette numbers that are put onto the processor so that in the event of some incident such as we had the retrieval of the exact specimens can be done electronically? And lastly can anyone tell me if they have a fully barcoded system whereby path specimens arrive barcoded and every document, slide and block has a barcode that allows for tracking of the tissue at all times? We are looking at processes and trying to close some gaps. Lynn Wade Program Manager, Safety & Quality Management Medical Services & Diagnostics Eastern Health From jnocito <@t> satx.rr.com Sun Mar 23 19:45:48 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sun Mar 23 19:45:35 2008 Subject: [Histonet] Reprocessing tissue References: <002401c88d3e$0d922730$0a02a8c0@wadeq8fg62kuom> Message-ID: <002e01c88d48$679922b0$0302a8c0@yourxhtr8hvc4p> Lynn, ThermoFisher Scientific and Ventana Medical Systems have systems that you can barcode the paperwork, which then makes bar-coded blocks which then makes bar-coded slides. In each step, the barcodes are read to ensure that the correct specimen is being processed. If there is a mis-match, an alarm beeps alerting the user. For reprocessing tissue, we just melt the blocks down, place the tissue back in the block and put the blocks in formalin to be processed with new cases. Whatever area did not process the first time will take up the formalin, and graded alcohols. When the tissues reach xylene, the paraffin is dissolved and everything get infiltrated. The areas that have been processed will repel the formalin and alcohols until they are immersed into xylene. I find this method is a lot easier on the tissues, especially if IHC is performed on them later. As far a an electronically created list of blocks going into a processor, I haven't heard of any. Hope this helps. Joe ----- Original Message ----- From: "Lynn Wade" To: Sent: Sunday, March 23, 2008 6:31 PM Subject: [Histonet] Reprocessing tissue Hi folks: I am wondering if anyone has had an incident in Patholgy lab where on the tissue processor the reagents got switched inadvertantly. For instance, the 80% alcohol was inadvertantly placed in the last 100% alcohol slot and thus water was reintroduced into the tissue just before xylene, clearing amd paraffin. Has anyone had this occur and how did you recover the tissue? Also, can anyone tell me if there is such a processor that has a system that can be used to log in the cassette numbers that are put onto the processor so that in the event of some incident such as we had the retrieval of the exact specimens can be done electronically? And lastly can anyone tell me if they have a fully barcoded system whereby path specimens arrive barcoded and every document, slide and block has a barcode that allows for tracking of the tissue at all times? We are looking at processes and trying to close some gaps. Lynn Wade Program Manager, Safety & Quality Management Medical Services & Diagnostics Eastern Health _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Mon Mar 24 04:13:30 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Mar 24 04:13:39 2008 Subject: AW: [Histonet] Reprocessing tissue In-Reply-To: <002e01c88d48$679922b0$0302a8c0@yourxhtr8hvc4p> Message-ID: <001201c88d8f$54a5f060$eeeea8c0@dielangs.at> Joe, How many blocks did you reprocess in this manner at one time? I am concerned, if the processor suffers from too many paraffinized blocks in the retorte. When we were trained on our first VIP (1989) the technician stressed the importance of getting rid of the remaining paraffin in the retorte before starting a new run. He said, the danger is, that little hard particles of paraffin can get into the valves and block it. What do you think about this? Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Joe Nocito Gesendet: Montag, 24. M?rz 2008 01:46 An: Lynn Wade; histonet@lists.utsouthwestern.edu Betreff: Re: [Histonet] Reprocessing tissue Lynn, ThermoFisher Scientific and Ventana Medical Systems have systems that you can barcode the paperwork, which then makes bar-coded blocks which then makes bar-coded slides. In each step, the barcodes are read to ensure that the correct specimen is being processed. If there is a mis-match, an alarm beeps alerting the user. For reprocessing tissue, we just melt the blocks down, place the tissue back in the block and put the blocks in formalin to be processed with new cases. Whatever area did not process the first time will take up the formalin, and graded alcohols. When the tissues reach xylene, the paraffin is dissolved and everything get infiltrated. The areas that have been processed will repel the formalin and alcohols until they are immersed into xylene. I find this method is a lot easier on the tissues, especially if IHC is performed on them later. As far a an electronically created list of blocks going into a processor, I haven't heard of any. Hope this helps. Joe ----- Original Message ----- From: "Lynn Wade" To: Sent: Sunday, March 23, 2008 6:31 PM Subject: [Histonet] Reprocessing tissue Hi folks: I am wondering if anyone has had an incident in Patholgy lab where on the tissue processor the reagents got switched inadvertantly. For instance, the 80% alcohol was inadvertantly placed in the last 100% alcohol slot and thus water was reintroduced into the tissue just before xylene, clearing amd paraffin. Has anyone had this occur and how did you recover the tissue? Also, can anyone tell me if there is such a processor that has a system that can be used to log in the cassette numbers that are put onto the processor so that in the event of some incident such as we had the retrieval of the exact specimens can be done electronically? And lastly can anyone tell me if they have a fully barcoded system whereby path specimens arrive barcoded and every document, slide and block has a barcode that allows for tracking of the tissue at all times? We are looking at processes and trying to close some gaps. Lynn Wade Program Manager, Safety & Quality Management Medical Services & Diagnostics Eastern Health _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Mon Mar 24 04:26:55 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Mon Mar 24 04:26:59 2008 Subject: [Histonet] Reprocessing tissue In-Reply-To: <001201c88d8f$54a5f060$eeeea8c0@dielangs.at> References: <002e01c88d48$679922b0$0302a8c0@yourxhtr8hvc4p> <001201c88d8f$54a5f060$eeeea8c0@dielangs.at> Message-ID: <5b6eb13e0803240226q326a798tcfe3bd0da2dca144@mail.gmail.com> You might want to scrape off any extra paraffin and make sure a cleaning cycle has been run, but I think Joe has it right when he says that this is the way to reprocess tissue. Clean the bottles and the processor after the run, but for the sake of the tissue, don't deparaffinize the tissue before reprocessing. Keep in mind that he is melting any extra paraffin off before processing. When he puts the cassettes in the container of formalin, any little bits of paraffin probably float up to the top. I'd think build-up in the machine shouldn't be much of a problem. On Mon, Mar 24, 2008 at 2:13 AM, Gudrun Lang wrote: > Joe, > How many blocks did you reprocess in this manner at one time? I am > concerned, if the processor suffers from too many paraffinized blocks in > the > retorte. > When we were trained on our first VIP (1989) the technician stressed the > importance of getting rid of the remaining paraffin in the retorte before > starting a new run. He said, the danger is, that little hard particles of > paraffin can get into the valves and block it. > What do you think about this? > > Gudrun Lang > > Biomed. Analytikerin > Histolabor > Akh Linz > Krankenhausstr. 9 > 4020 Linz > +43(0)732/7806-6754 > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Joe > Nocito > Gesendet: Montag, 24. M?rz 2008 01:46 > An: Lynn Wade; histonet@lists.utsouthwestern.edu > Betreff: Re: [Histonet] Reprocessing tissue > > Lynn, > ThermoFisher Scientific and Ventana Medical Systems have systems that you > can barcode the paperwork, which then makes bar-coded blocks which then > makes bar-coded slides. In each step, the barcodes are read to ensure that > the correct specimen is being processed. If there is a mis-match, an alarm > beeps alerting the user. > > For reprocessing tissue, we just melt the blocks down, place the tissue > back > > in the block and put the blocks in formalin to be processed with new > cases. > Whatever area did not process the first time will take up the formalin, > and > graded alcohols. When the tissues reach xylene, the paraffin is dissolved > and everything get infiltrated. The areas that have been processed will > repel the formalin and alcohols until they are immersed into xylene. I > find > this method is a lot easier on the tissues, especially if IHC is performed > on them later. > > As far a an electronically created list of blocks going into a processor, > I > haven't heard of any. > > Hope this helps. > > Joe > ----- Original Message ----- > From: "Lynn Wade" > To: > Sent: Sunday, March 23, 2008 6:31 PM > Subject: [Histonet] Reprocessing tissue > > > Hi folks: > I am wondering if anyone has had an incident in Patholgy lab where on the > tissue processor the reagents got switched inadvertantly. For instance, > the > 80% alcohol was inadvertantly placed in the last 100% alcohol slot and > thus > water was reintroduced into the tissue just before xylene, clearing amd > paraffin. > > Has anyone had this occur and how did you recover the tissue? > > Also, can anyone tell me if there is such a processor that has a system > that > > can be used to log in the cassette numbers that are put onto the processor > so that in the event of some incident such as we had the retrieval of the > exact specimens can be done electronically? > > And lastly can anyone tell me if they have a fully barcoded system whereby > path specimens arrive barcoded and every document, slide and block has a > barcode that allows for tracking of the tissue at all times? > > We are looking at processes and trying to close some gaps. > > Lynn Wade > Program Manager, Safety & Quality Management > Medical Services & Diagnostics > Eastern Health > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From kemlo <@t> f2s.com Mon Mar 24 04:31:28 2008 From: kemlo <@t> f2s.com (kemlo) Date: Mon Mar 24 04:33:06 2008 Subject: [Histonet] Reprocessing tissue In-Reply-To: <002e01c88d48$679922b0$0302a8c0@yourxhtr8hvc4p> References: <002401c88d3e$0d922730$0a02a8c0@wadeq8fg62kuom> <002e01c88d48$679922b0$0302a8c0@yourxhtr8hvc4p> Message-ID: <3391497E7391401396A345F0D88D81D6@KemloPC> Um no, Joe..... If you do that then the xylene/ wax impregnated tissue won't mix with the formalin (as you say), anyway it's fixed that's not the issue, so why waste time fixing again?. Melt the blocks down, as you say, then pass through xylem until they are transparent, well as transparent as the poor processing allows. You then place into 100% alcohol until properly dehydrated denoted by the xylene making them transparent (trial and error I'm afraid as you don't know how dehydrated they are). When clear impregnate with wax as before; you may note that the tissue becomes more brittle because of increased length of time in reagents and especially wax. But less so than Joe says as you are not doubling the processing time which must impact adversely on ICC. Have fun. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: 24 March 2008 00:46 To: Lynn Wade; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Reprocessing tissue Lynn, ThermoFisher Scientific and Ventana Medical Systems have systems that you can barcode the paperwork, which then makes bar-coded blocks which then makes bar-coded slides. In each step, the barcodes are read to ensure that the correct specimen is being processed. If there is a mis-match, an alarm beeps alerting the user. For reprocessing tissue, we just melt the blocks down, place the tissue back in the block and put the blocks in formalin to be processed with new cases. Whatever area did not process the first time will take up the formalin, and graded alcohols. When the tissues reach xylene, the paraffin is dissolved and everything get infiltrated. The areas that have been processed will repel the formalin and alcohols until they are immersed into xylene. I find this method is a lot easier on the tissues, especially if IHC is performed on them later. As far a an electronically created list of blocks going into a processor, I haven't heard of any. Hope this helps. Joe ----- Original Message ----- From: "Lynn Wade" To: Sent: Sunday, March 23, 2008 6:31 PM Subject: [Histonet] Reprocessing tissue Hi folks: I am wondering if anyone has had an incident in Patholgy lab where on the tissue processor the reagents got switched inadvertantly. For instance, the 80% alcohol was inadvertantly placed in the last 100% alcohol slot and thus water was reintroduced into the tissue just before xylene, clearing amd paraffin. Has anyone had this occur and how did you recover the tissue? Also, can anyone tell me if there is such a processor that has a system that can be used to log in the cassette numbers that are put onto the processor so that in the event of some incident such as we had the retrieval of the exact specimens can be done electronically? And lastly can anyone tell me if they have a fully barcoded system whereby path specimens arrive barcoded and every document, slide and block has a barcode that allows for tracking of the tissue at all times? We are looking at processes and trying to close some gaps. Lynn Wade Program Manager, Safety & Quality Management Medical Services & Diagnostics Eastern Health _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo <@t> f2s.com Mon Mar 24 04:33:42 2008 From: kemlo <@t> f2s.com (kemlo) Date: Mon Mar 24 04:35:13 2008 Subject: [Histonet] Reprocessing tissue In-Reply-To: <001201c88d8f$54a5f060$eeeea8c0@dielangs.at> References: <002e01c88d48$679922b0$0302a8c0@yourxhtr8hvc4p> <001201c88d8f$54a5f060$eeeea8c0@dielangs.at> Message-ID: <6C75EC17B23E464983135795F49CEB4B@KemloPC> I would reprocess according to what I've said, by hand. You are correct putting the cloths through the washer twice could contaminate the washer. Personally I like to hand wash my cloths; takes time but you get a cleaner white. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: 24 March 2008 09:14 To: 'Joe Nocito' Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] Reprocessing tissue Joe, How many blocks did you reprocess in this manner at one time? I am concerned, if the processor suffers from too many paraffinized blocks in the retorte. When we were trained on our first VIP (1989) the technician stressed the importance of getting rid of the remaining paraffin in the retorte before starting a new run. He said, the danger is, that little hard particles of paraffin can get into the valves and block it. What do you think about this? Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Joe Nocito Gesendet: Montag, 24. M?rz 2008 01:46 An: Lynn Wade; histonet@lists.utsouthwestern.edu Betreff: Re: [Histonet] Reprocessing tissue Lynn, ThermoFisher Scientific and Ventana Medical Systems have systems that you can barcode the paperwork, which then makes bar-coded blocks which then makes bar-coded slides. In each step, the barcodes are read to ensure that the correct specimen is being processed. If there is a mis-match, an alarm beeps alerting the user. For reprocessing tissue, we just melt the blocks down, place the tissue back in the block and put the blocks in formalin to be processed with new cases. Whatever area did not process the first time will take up the formalin, and graded alcohols. When the tissues reach xylene, the paraffin is dissolved and everything get infiltrated. The areas that have been processed will repel the formalin and alcohols until they are immersed into xylene. I find this method is a lot easier on the tissues, especially if IHC is performed on them later. As far a an electronically created list of blocks going into a processor, I haven't heard of any. Hope this helps. Joe ----- Original Message ----- From: "Lynn Wade" To: Sent: Sunday, March 23, 2008 6:31 PM Subject: [Histonet] Reprocessing tissue Hi folks: I am wondering if anyone has had an incident in Patholgy lab where on the tissue processor the reagents got switched inadvertantly. For instance, the 80% alcohol was inadvertantly placed in the last 100% alcohol slot and thus water was reintroduced into the tissue just before xylene, clearing amd paraffin. Has anyone had this occur and how did you recover the tissue? Also, can anyone tell me if there is such a processor that has a system that can be used to log in the cassette numbers that are put onto the processor so that in the event of some incident such as we had the retrieval of the exact specimens can be done electronically? And lastly can anyone tell me if they have a fully barcoded system whereby path specimens arrive barcoded and every document, slide and block has a barcode that allows for tracking of the tissue at all times? We are looking at processes and trying to close some gaps. Lynn Wade Program Manager, Safety & Quality Management Medical Services & Diagnostics Eastern Health _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Mon Mar 24 09:30:18 2008 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Mon Mar 24 09:30:39 2008 Subject: [Histonet] Histology Atlas In-Reply-To: <2914.65.40.219.161.1206147834.squirrel@host7.wfdns.com> References: <47DE536C.C068.0078.0@hsc.wvu.edu> <47DE935C.8000803@umdnj.edu> <2914.65.40.219.161.1206147834.squirrel@host7.wfdns.com> Message-ID: <47E7BAFA.9000907@umdnj.edu> The third edition of Ross. Romrell and Kaye was very good, the 4th edition was terrible. I reviewed it for the publisher and was shocked at how they had ruined a good book. Even though it was billed as a "text and atlas" the atlas figures were never mentioned in the text!? I have not seen the 5th edition but histology has not changed much! Most new editions just add some cute stuff and/or change italics to bold face type in hopes of getting buyers to "upgrade". Geoff anthony@histotechexchange.com wrote: > Dear Kimberly: > > I am a big fan of "Histology A Text and Atlas" by Michael H. Ross and Lynn > J. Romrell (ISBN 0-683-07368-0). It is the book I used in university in > the UK but it is an American book. Make sure you find the latest edition; > the ISBN number is for the second edition. > > Yours truly, > > Anthony Williams BSc. HT > Histotech Exchange LLC > 19 Whitmore St. > Lexington, VA 24450 > T 1 (302) 383 9780 > F 1 (540) 463 3583 > anthony@histotechexchange.com > > > > I second the choices Jennifer mentioned. We use both of those. > >> Geoff >> >> Kimberly Secrest wrote: >> >>> Can anyone recommend a really good histology atlas. It will be used for >>> teaching new students. >>> Thanks >>> Kim >>> >>> Kimberly Secrest, HTL, QIHC >>> Instructor >>> Department of Pathology >>> West Virginia University >>> 304-293-7628 >>> >>> >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >>> >>> >> -- >> -- >> ********************************************** >> Geoff McAuliffe, Ph.D. >> Neuroscience and Cell Biology >> Robert Wood Johnson Medical School >> 675 Hoes Lane, Piscataway, NJ 08854 >> voice: (732)-235-4583 >> mcauliff@umdnj.edu >> ********************************************** >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From GDawson <@t> dynacaremilwaukee.com Mon Mar 24 09:50:26 2008 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Mon Mar 24 09:50:37 2008 Subject: [Histonet] p57 In-Reply-To: <47E7BAFA.9000907@umdnj.edu> Message-ID: All, I deleted an email that was inquiring into a good IVD p57 antibody so I'll have to send this to everyone...sorry. To whom it may concern, a good IVD p57 antibody is the mouse monoclonal from visionbiosystems/novocastra (cat# NCL-p57). I do it at a 1:150 dilution following HIER in pH 6.0 citrate target retieval. Best of Luck, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI From ploykasek <@t> phenopath.com Mon Mar 24 10:20:52 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Mon Mar 24 10:21:07 2008 Subject: [Histonet] p57 In-Reply-To: Message-ID: We are using clone 57P06(KP10) from Thermo/Neomarkers/Labvision (I hope I got all the names in there!). Like Glen, we use it with a citrate heat antigen retrieval. It can be purchased as an IVD. By the way Glen, don't forget to add Leica to the long list of names before Novocastra! Happy Monday to all. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > All, > > I deleted an email that was inquiring into a good IVD p57 antibody so I'll > have to send this to everyone...sorry. > > To whom it may concern, a good IVD p57 antibody is the mouse monoclonal from > visionbiosystems/novocastra (cat# NCL-p57). > > I do it at a 1:150 dilution following HIER in pH 6.0 citrate target retieval. > > Best of Luck, > > Glen Dawson BS, HT & QIHC (ASCP) > IHC Manager > Milwaukee, WI > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From igor.deyneko <@t> gmail.com Mon Mar 24 10:31:48 2008 From: igor.deyneko <@t> gmail.com (Igor Deyneko) Date: Mon Mar 24 10:32:00 2008 Subject: [Histonet] Alkaline Phospahtase and Methyl Green Message-ID: <35e16a770803240831h13a00090yc0b9242dfee3fc14@mail.gmail.com> Dear Histonetters! I was wondering if anyone can help me out. I'm doing IHC with Ach antibodies and when I use DAB chromogen I get strong background. I used Vulcan Red (from Biocare Medical), which got rid of the background, but it's a bit hard to distinguish from Hematoxylin counterstain that I use. I was wondering if Methyl Green would be an appropriate counterstain for it? I tried using 0.5%Methyl Green from Plyscientific before, but had problems because it washed off right away in the first change of 95 Ethanol. I also tried n-butanol and acetone, but the results were unsatisfactory. If anyone knows good percentage to use as a counterstain and a protocol would be greatly appreciated. Thank you in advance. Igor Deyneko Infinity Pharmaceuticals In-Vivo Pharmacology Cambridge, MA From minhan.tan <@t> gmail.com Mon Mar 24 11:15:59 2008 From: minhan.tan <@t> gmail.com (Min-Han Tan) Date: Mon Mar 24 11:16:04 2008 Subject: [Histonet] ERCC1 IHC Message-ID: <920cc4a70803240915w622855f1m32543037d863e869@mail.gmail.com> Dear fellow Histonetters, I am planning to start immunostaining FFPE lung tissue for ERCC1. The cited antibody in the relevant New England Journal of Medicinepaper ERCC1 Ab-2 (Clone 8F1), Neomarkers Cat. #MS-671-R7 (7.0ml) did not work well for us in the prediluted form, and I understand the prediluted form has been withdrawn by the manufacturer as well. I would like to ask as to which antibody all of you are using for ERCC1 staining (lung cancer tissue for platinum sensitivity). I understand some clinical laboratories in the United States are using it on a routine basis. I am thinking of obtaining the Abcam one (with the same clone 8F1, approved for IHC - it might even be the same antibody as the Neomarkers one!) Thank you! -- /min-han From Steven.McLean <@t> crl.com Mon Mar 24 11:21:36 2008 From: Steven.McLean <@t> crl.com (McLean, Steven) Date: Mon Mar 24 11:21:43 2008 Subject: [Histonet] CD348 Message-ID: <00CE31073BB3554A9C0D6FEB3BDA704B0CFCB5@edimpeml0001.eu01.crl.com> Does anyone know of a CD348 Ab suitable for use in IHC for frozen or preferably FFPE (non) Human primate tissue?
This email, its contents and attachments are confidential and may be covered by legal privilege. This email contains information intended only for the person(s) and/or entity named above. The views and opinions expressed are those of the sender and not necessarily those of Charles River Laboratories or its affiliates. Any other distribution, copying, review, use or disclosure is strictly prohibited. If you are not the intended recipient, please delete this message and any attachments without making a copy and advise the sender by return email - thank you.

This email and any attachments have been scanned for viruses prior to leaving the Charles River Laboratories network. Charles River Laboratories will not be liable for direct, special, indirect or consequential damages arising from alteration of the contents of this message by a third party or as a result of any virus being passed on. For more information on the range of services that we offer, please visit our website at http://www.criver.com
From octavio109 <@t> hotmail.com  Mon Mar 24 14:26:37 2008
From: octavio109 <@t> hotmail.com (Corinthia D. Emanuel)
Date: Mon Mar 24 14:26:40 2008
Subject: [Histonet] RE: Histonet Digest, Vol 52, Issue 36
Message-ID: 








Altanta, Georgia dermatopathology lab seeks histotechs in all shifts for both full & part time work schedules.    The ability to embed, cut, gross and stain tissue is highly desireable.  HT credential preferred. 
 
Fax resume immediately to:  770-381-6451.  Competitive salary & flexible schedule available.  
_________________________________________________________________
In a rush?  Get real-time answers with Windows Live Messenger.
http://www.windowslive.com/messenger/overview.html?ocid=TXT_TAGLM_WL_Refresh_realtime_042008
From jennifer.l.hofecker <@t> Vanderbilt.Edu  Mon Mar 24 15:16:31 2008
From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L)
Date: Mon Mar 24 15:16:40 2008
Subject: [Histonet] Tennessee Society for Histotechnology
Message-ID: <898D946569A27444B65667A49C0740520175B1B9@mailbe06.mc.vanderbilt.edu>

Happy Monday to all!
The program for the TSH meeting in June has been mailed to members.  You
may also access it online via the NSH website ( www.nsh.org ) under
state meetings. The meeting is June 19-21 in beautiful Townsend, TN.
 
Just a brief preview of our educational offerings:
The Essential Role of Histotechnologists in the Diagnosis of Pediatric
Solid Tumors, Antibodies 2008, Maintaining Specimen Integrity in the
Laboratory, The Leadership Pickles, The Wonderful World of
Neuropathology, Applications of IHC in Dermatopathology, Laboratory
Management Problems and Solutions, The Most Elegant of Genetic Tests,
Rodent Detection Workshop, Who's RITA and Why We Should get to Know Her,
Histology Equipment Repair and Maintenance, Human Anatomy and
Physiology: What a Histotech Should Know, Guidelines & General
Procedures for Hazardous Chemicals Within the Histopathology Laboratory.
 
Please see the complete program on the NSH web page.
If you need additional information, please contact Rhonda Schalk @
turbospider@juno.com or you may contact me at the email address listed
below.
Thanks and everyone have a Great week!
jennifer
 
Jennifer L. Hofecker, HT(ASCP)
Vanderbilt University Medical Center
Division of Neuropathology 
Nashville, TN
ph. (615)343-0083
fax. (615)343-7089
NSH Quality Control Committee Chair
Tennessee Society for Histotechnology Secretary
2008 TSH Meeting Exhibit Coordinator
 
 
From podpath <@t> bellsouth.net  Mon Mar 24 16:03:15 2008
From: podpath <@t> bellsouth.net (podpath@bellsouth.net)
Date: Mon Mar 24 16:03:21 2008
Subject: [Histonet] Positions in Alpharetta, GA
Message-ID: <032420082103.13091.47E8171300061C750000332322230650029B0A02D2089B9A019C04040A0DBF089B0E9F0B019F@att.net>

Exciting job opportunity with a new reference lab in Alpharetta, GA. Lab will open summer 2008. Currently, there are two histotechnologist positions open. Applicants should be HTL or HT certified. Job description includes general histology including experience with manual special stains and immunos.

The lab is offering a very competitive benefits package and very congenial work environment. Other positions also available include data entry and pathology/medical transcription. Interested parties should respond with resume or inquiry to podpath@bellsouth.net. 

If you want to be part of something new with excellent growth potential and a family like atmosphere, then this job may be for you.
From jnocito <@t> satx.rr.com  Mon Mar 24 18:23:00 2008
From: jnocito <@t> satx.rr.com (Joe Nocito)
Date: Mon Mar 24 18:23:06 2008
Subject: [Histonet] Reprocessing tissue
References: <002401c88d3e$0d922730$0a02a8c0@wadeq8fg62kuom>
	<002e01c88d48$679922b0$0302a8c0@yourxhtr8hvc4p>
	<3391497E7391401396A345F0D88D81D6@KemloPC>
Message-ID: <004301c88e06$007d2a90$0302a8c0@yourxhtr8hvc4p>

Kemlo,
um, no. Deparaffinizing, dehydrating and then going back through paraffin is 
too harsh on the tissue. For argument sake, try it out. I've been doing this 
for years and haven't had a problem.

Joe
----- Original Message ----- 
From: "kemlo" 
To: "'Joe Nocito'" ; "'Lynn Wade'" 
; 
Sent: Monday, March 24, 2008 4:31 AM
Subject: RE: [Histonet] Reprocessing tissue


> Um no, Joe.....
>
> If you do that then the xylene/ wax impregnated tissue won't mix with the
> formalin (as you say), anyway it's fixed that's not the issue, so why 
> waste
> time fixing again?. Melt the blocks down, as you say, then pass through
> xylem until they are transparent, well as transparent as the poor 
> processing
> allows. You then place into 100% alcohol until properly dehydrated denoted
> by the xylene making them transparent (trial and error I'm afraid as you
> don't know how dehydrated they are). When clear impregnate with wax as
> before; you may note that the tissue becomes more brittle because of
> increased length of time in reagents and especially wax. But less so than
> Joe says as you are not doubling the processing time which must impact
> adversely on ICC.
>
> Have fun.
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito
> Sent: 24 March 2008 00:46
> To: Lynn Wade; histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Reprocessing tissue
>
> Lynn,
> ThermoFisher Scientific and Ventana Medical Systems have systems that you
> can barcode the paperwork, which then makes bar-coded blocks which then
> makes bar-coded slides. In each step, the barcodes are read to ensure that
> the correct specimen is being processed. If there is a mis-match, an alarm
> beeps alerting the user.
>
> For reprocessing tissue, we just melt the blocks down, place the tissue 
> back
>
> in the block and put the blocks in formalin to be processed with new 
> cases.
> Whatever area did not process the first time will take up the formalin, 
> and
> graded alcohols. When the tissues reach xylene, the paraffin is dissolved
> and everything get infiltrated. The areas that have been processed will
> repel the formalin and alcohols until they are immersed into xylene. I 
> find
> this method is a lot easier on the tissues, especially if IHC is performed
> on them later.
>
> As far a an electronically created list of blocks going into a processor, 
> I
> haven't heard of any.
>
> Hope this helps.
>
> Joe
> ----- Original Message ----- 
> From: "Lynn Wade" 
> To: 
> Sent: Sunday, March 23, 2008 6:31 PM
> Subject: [Histonet] Reprocessing tissue
>
>
> Hi folks:
> I am wondering if anyone has had an incident in Patholgy lab where on the
> tissue processor the reagents got switched inadvertantly. For instance, 
> the
> 80% alcohol was inadvertantly placed in the last 100% alcohol slot and 
> thus
> water was reintroduced into the tissue just before xylene, clearing amd
> paraffin.
>
> Has anyone had this occur and how did you recover the tissue?
>
> Also, can anyone tell me if there is such a processor that has a system 
> that
>
> can be used to log in the cassette numbers that are put onto the processor
> so that in the event of some incident such as we had the retrieval of the
> exact specimens can be done electronically?
>
> And lastly can anyone tell me if they have a fully barcoded system whereby
> path specimens arrive barcoded and every document, slide and block has a
> barcode that allows for tracking of the tissue at all times?
>
> We are looking at processes and trying to close some gaps.
>
> Lynn Wade
> Program Manager, Safety & Quality Management
> Medical Services & Diagnostics
> Eastern Health
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> 


From tinahammel <@t> comcast.net  Mon Mar 17 08:06:15 2008
From: tinahammel <@t> comcast.net (TIna Hammel)
Date: Mon Mar 24 19:49:53 2008
Subject: [Histonet] (no subject)
Message-ID: <000001c8882f$afb1ba80$6400a8c0@goldhawaii>

My husband and I will be relocating to Delaware in 45 days.  I have been
a Histology Tech in the Air Force for 10 years.  My husband has been a
Histology Tech and also an EM tech for 6 years.  My husband is
certified.  If you know of any jobs in the Delaware area or Eastern
Shore area please respond.
 
Thank you for your time,
Tina Hammel
From kemlo <@t> f2s.com  Tue Mar 25 02:59:13 2008
From: kemlo <@t> f2s.com (kemlo)
Date: Tue Mar 25 03:00:52 2008
Subject: [Histonet] Reprocessing tissue
In-Reply-To: <000001c88e11$0293fba0$6901a8c0@FSROGER>
References: <002401c88d3e$0d922730$0a02a8c0@wadeq8fg62kuom><002e01c88d48$679922b0$0302a8c0@yourxhtr8hvc4p><3391497E7391401396A345F0D88D81D6@KemloPC>
	<004301c88e06$007d2a90$0302a8c0@yourxhtr8hvc4p>
	<000001c88e11$0293fba0$6901a8c0@FSROGER>
Message-ID: <3B3F251DE4B3433D9E9CFF0966A2DFE6@KemloPC>


There appears to be a unanimous American response that I'm wrong so I'll
have to accept that.


However, that's how I used to salvage poorly processed samples in the UK,
but I do concede that was before the advent of these new fangled washing
machine processors in the days of Shandon.

Don't suppose anyone processes brains in methyl salicylate after protracted
processing in ascending grades of alcohols anymore in those big brain jars?
Or process Gough and Wentworth lung sections, or prepare museum jars? Will
Histology become like Blood Science? Big grey machines manned by
Technicians?

So why are we called Scientists? Confuses me loads and it scares me that I
sound like my old Senior Chief in Histopathology, before we became Cellular
Pathology.    

-----Original Message-----
From: Cheryl [mailto:tkngflght@yahoo.com] 
Sent: 25 March 2008 00:42
To: 'kemlo'
Subject: RE: [Histonet] Reprocessing tissue

 
Hey Kemlo-

We've done this in dozens of labs and it works...can't explain why you don't
have to dehydrate more (probably the heat and extra time) but it's gentler
on small biopsies and skins.  Bigger stuff like colon and breast probably
merits the whole shebang, but melting into cassettes and putting on the
machine again with no other handling for the little stuff works like a
charm.

Cheryl Kerry
Full Staff Inc...

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito
Sent: Monday, March 24, 2008 6:23 PM
To: kemlo; 'Lynn Wade'; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Reprocessing tissue

Kemlo,
um, no. Deparaffinizing, dehydrating and then going back through paraffin is
too harsh on the tissue. For argument sake, try it out. I've been doing this
for years and haven't had a problem.

Joe
----- Original Message -----
From: "kemlo" 
To: "'Joe Nocito'" ; "'Lynn Wade'" 
; 
Sent: Monday, March 24, 2008 4:31 AM
Subject: RE: [Histonet] Reprocessing tissue


> Um no, Joe.....
>
> If you do that then the xylene/ wax impregnated tissue won't mix with the
> formalin (as you say), anyway it's fixed that's not the issue, so why 
> waste
> time fixing again?. Melt the blocks down, as you say, then pass through
> xylem until they are transparent, well as transparent as the poor 
> processing
> allows. You then place into 100% alcohol until properly dehydrated denoted
> by the xylene making them transparent (trial and error I'm afraid as you
> don't know how dehydrated they are). When clear impregnate with wax as
> before; you may note that the tissue becomes more brittle because of
> increased length of time in reagents and especially wax. But less so than
> Joe says as you are not doubling the processing time which must impact
> adversely on ICC.
>
> Have fun.
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito
> Sent: 24 March 2008 00:46
> To: Lynn Wade; histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Reprocessing tissue
>
> Lynn,
> ThermoFisher Scientific and Ventana Medical Systems have systems that you
> can barcode the paperwork, which then makes bar-coded blocks which then
> makes bar-coded slides. In each step, the barcodes are read to ensure that
> the correct specimen is being processed. If there is a mis-match, an alarm
> beeps alerting the user.
>
> For reprocessing tissue, we just melt the blocks down, place the tissue 
> back
>
> in the block and put the blocks in formalin to be processed with new 
> cases.
> Whatever area did not process the first time will take up the formalin, 
> and
> graded alcohols. When the tissues reach xylene, the paraffin is dissolved
> and everything get infiltrated. The areas that have been processed will
> repel the formalin and alcohols until they are immersed into xylene. I 
> find
> this method is a lot easier on the tissues, especially if IHC is performed
> on them later.
>
> As far a an electronically created list of blocks going into a processor, 
> I
> haven't heard of any.
>
> Hope this helps.
>
> Joe
> ----- Original Message ----- 
> From: "Lynn Wade" 
> To: 
> Sent: Sunday, March 23, 2008 6:31 PM
> Subject: [Histonet] Reprocessing tissue
>
>
> Hi folks:
> I am wondering if anyone has had an incident in Patholgy lab where on the
> tissue processor the reagents got switched inadvertantly. For instance, 
> the
> 80% alcohol was inadvertantly placed in the last 100% alcohol slot and 
> thus
> water was reintroduced into the tissue just before xylene, clearing amd
> paraffin.
>
> Has anyone had this occur and how did you recover the tissue?
>
> Also, can anyone tell me if there is such a processor that has a system 
> that
>
> can be used to log in the cassette numbers that are put onto the processor
> so that in the event of some incident such as we had the retrieval of the
> exact specimens can be done electronically?
>
> And lastly can anyone tell me if they have a fully barcoded system whereby
> path specimens arrive barcoded and every document, slide and block has a
> barcode that allows for tracking of the tissue at all times?
>
> We are looking at processes and trying to close some gaps.
>
> Lynn Wade
> Program Manager, Safety & Quality Management
> Medical Services & Diagnostics
> Eastern Health
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> 


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet






From c.m.vanderloos <@t> amc.uva.nl  Tue Mar 25 03:01:04 2008
From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos)
Date: Tue Mar 25 03:01:20 2008
Subject: [Histonet] RE: Alkaline Phospahtase and Methyl Green
Message-ID: <9067c2812ede6734.47e8bf50@amc.uva.nl>

Igor,The fact that Vulcan Red provides a good result whereas DAB showed strong background might be explained by the lower staining sensitivity/efficiency of the Vulcan Red compared with DAB. Have you tried to further dilute your primary antibody when using DAB?Using Vulcan Red as chromogen counterstained with hematoxylin usually gives an excellent result. Take care that the hematoxylin counterstain is not too strong and overwhelming the red reaction product. To prevent overstaining with hematoxylin dilute the standard Mayer's hematoxylin 1:10 with tap water and stain for 3-5 min. If you want to go for methylgreen, purchase Sigma M6776 (which is basically 'ethylgreen') and prepare a 0.1% solution in a sodium acetate buffer (0.1 M, pH5.2). First, incubate your slides with the sodium acetate buffer for 10-15 min, blott off and stain with the ethylgreen solution for 5 min. Wash with tap water (5 min) and dry slides at a hot plate. Coverslip with an organic mounting medium without xylene or alcohol (for example: VectaMount, cat.no. H-5000)Lots of success!Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center M2-230
Meibergdreef 9
NL-1105 AZ Amsterdam
The Netherlands Date: Mon, 24 Mar 2008 11:31:48 -0400
From: "Igor Deyneko" 
Subject: [Histonet] Alkaline Phospahtase and Methyl Green
To: Histonet@lists.utsouthwestern.edu

Dear Histonetters!
I was wondering if anyone can help me out. I'm doing IHC with Ach antibodies
and when I use DAB chromogen I get strong background. I used Vulcan Red
(from Biocare Medical), which got rid of the background, but it's a bit hard
to distinguish from Hematoxylin counterstain that I use. I was wondering if
Methyl Green would be an appropriate counterstain for it? I tried
using 0.5%Methyl Green from Plyscientific before, but had problems
because it washed
off right away in the first change of 95 Ethanol. I also tried n-butanol and
acetone, but the results were unsatisfactory. If anyone knows good
percentage to use as a counterstain and a protocol would be greatly
appreciated.
Thank you in advance.
Igor Deyneko
Infinity Pharmaceuticals
In-Vivo Pharmacology
Cambridge, MA
From barbara.verstraeten <@t> ugent.be  Tue Mar 25 03:50:27 2008
From: barbara.verstraeten <@t> ugent.be (Barbara Verstraeten)
Date: Tue Mar 25 03:50:40 2008
Subject: [Histonet] embedding problem small zebrafish
Message-ID: <20080325095027.tzk9sa21ogg80so8@webmail.ugent.be>

Hello,

I work on zebrafish from 44 hours to 6 days old (so they are very  
small; max. 0.5 cm).
I would like to perform immostainings on them (such as E-cadherin  
staining). I've embedded my species in technovit 9100 but the cutting  
of such small animals is very difficult.
I did some stainings already and the antibody works but the problem is  
that my tissue is of very bad quality!! When I have 50 sections only 1  
is usefull.
This is not very good!

Can someone give me any suggestions on how to embed them so that they  
are more easy to section and that I can still stain them with  
different antibodies?

Thank you all in advance!

Kind regards,

Barbara Verstraeten
VMDB
University of Ghent
Belgium

From rydomsal <@t> yahoo.com  Tue Mar 25 03:58:16 2008
From: rydomsal <@t> yahoo.com (Ryan Dominique Salazar)
Date: Tue Mar 25 03:58:20 2008
Subject: [Histonet] slippery floor
Message-ID: <485235.24051.qm@web52303.mail.re2.yahoo.com>

Hi histopeeps!
   
  Do the common histo reagents/chemicals such as ethyl alcohol and xylene makes the floor slippery when you accidentally spilled them? I usually blame the paraffin wax as the culprit, though we do housekeeping before and after work.
  Our floor is made up of painted concrete pavement.
  Any solutions to remove the slippery thing before our QA will accidentally butt-kiss the floor.
   
  Thanx guys for all your help!
   
  Ryan Salazar
  Maccine Pte Ltd

       
---------------------------------
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From TownsendD <@t> childrensdayton.org  Tue Mar 25 07:35:21 2008
From: TownsendD <@t> childrensdayton.org (Dolores Townsend)
Date: Tue Mar 25 07:35:48 2008
Subject: [Histonet] Reprocessing tissue
Message-ID: 

Well, actually...
Somewhere, somehow, a Scientist who was also a Histology Technician was
reprocessing tissue the way you do, through xylene and alcohol, when
(s)he looked at the cleaning cycle on the processor: xylene, than
alcohol. And (s)he thought: "Mmmm, I wonder..."
So (s)he tried it and it worked.
Than another Scientist, who happened to be a Histology Technician,
thought: "Is it really necessary to clear the tissue before reprocessing
it?"
And (s)he tried, and it worked. So they wrote a paper about it to let
everyone know...
No, Science is not dead. Someone had to come up with the automated
processor, and the vacuum, and the microwave staining and processing. I
am sure that the future will still show new improvements. But it's going
to be very hard to embed or cut or troubleshoot the staining without a
Histology Technician, who also has to be a Scientist.
Dolores
From rjbuesa <@t> yahoo.com  Tue Mar 25 08:07:34 2008
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Tue Mar 25 08:07:38 2008
Subject: [Histonet] slippery floor
In-Reply-To: <485235.24051.qm@web52303.mail.re2.yahoo.com>
Message-ID: <715290.42275.qm@web65701.mail.ac4.yahoo.com>

The problem is the concrete because it has small irregularities where the paraffin gets trapped, specially after trying to eliminate it with paraffin solvents (the dissolved paraffin gets trapped in the irregularities).
  So the alcohol or the xylene does not make the tissue slippery, but the paraffin/xylene mixture that gets inside all the floor irregularities.
  You will have to totally eliminate the paraffin and its mixtures to eliminate this problem.
  Ren? J.

Ryan Dominique Salazar  wrote:
  Hi histopeeps!

Do the common histo reagents/chemicals such as ethyl alcohol and xylene makes the floor slippery when you accidentally spilled them? I usually blame the paraffin wax as the culprit, though we do housekeeping before and after work.
Our floor is made up of painted concrete pavement.
Any solutions to remove the slippery thing before our QA will accidentally butt-kiss the floor.

Thanx guys for all your help!

Ryan Salazar
Maccine Pte Ltd


---------------------------------
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From Heather.D.Renko <@t> osfhealthcare.org  Tue Mar 25 08:18:45 2008
From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.)
Date: Tue Mar 25 08:19:04 2008
Subject: [Histonet] re: purchasing new cryostats-opinions wanted
Message-ID: <40026EDDE64CDA47AB382C52619ACD3C0A09DE0D@pmc-rfd-mx01.intranet.osfnet.org>

Hello fellow Histotechs,

I am in the market to purchase two new cryostats in my 2008-2009 capital year and wanted your thoughts on instruments, installation experiences and overall thoughts on the products available.  Please email me personally on your experience's with the latest and greatest brands in the market.  

Heather D. Renko, Histology Coordinator
OSF Saint Anthony Medical Center
5666 East State Street
Rockford, Illinois 61108
Direct: 815-395-5410
Heather.D.Renko@osfhealthcare.org


==============================================================================
The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies.
==============================================================================
From kemlo <@t> f2s.com  Tue Mar 25 08:19:39 2008
From: kemlo <@t> f2s.com (kemlo)
Date: Tue Mar 25 08:21:17 2008
Subject: [Histonet] Reprocessing tissue
In-Reply-To: 
References: 
Message-ID: <424B30AFC03D459FBCD40346ECC26EFB@KemloPC>

Still looks like a washing machine.

 

  _____  

From: Dolores Townsend [mailto:TownsendD@childrensdayton.org] 
Sent: 25 March 2008 12:35
To: kemlo; 'Joe Nocito'; 'Cheryl'
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Reprocessing tissue

 

Well, actually...

Somewhere, somehow, a Scientist who was also a Histology Technician was
reprocessing tissue the way you do, through xylene and alcohol, when (s)he
looked at the cleaning cycle on the processor: xylene, than alcohol. And
(s)he thought: "Mmmm, I wonder..."

So (s)he tried it and it worked.

Than another Scientist, who happened to be a Histology Technician, thought:
"Is it really necessary to clear the tissue before reprocessing it?"

And (s)he tried, and it worked. So they wrote a paper about it to let
everyone know...

No, Science is not dead. Someone had to come up with the automated
processor, and the vacuum, and the microwave staining and processing. I am
sure that the future will still show new improvements. But it's going to be
very hard to embed or cut or troubleshoot the staining without a Histology
Technician, who also has to be a Scientist.

Dolores

From Heather.D.Renko <@t> osfhealthcare.org  Tue Mar 25 08:47:40 2008
From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.)
Date: Tue Mar 25 08:47:53 2008
Subject: [Histonet] re: belair
Message-ID: <40026EDDE64CDA47AB382C52619ACD3C06612DCA@pmc-rfd-mx01.intranet.osfnet.org>

Anyone have the right spelling and or website for Belair?

Heather D. Renko, Histology Coordinator
OSF Saint Anthony Medical Center
5666 East State Street
Rockford, Illinois 61108
Direct: 815-395-5410
Heather.D.Renko@osfhealthcare.org


==============================================================================
The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies.
==============================================================================
From cmiller <@t> physlab.com  Tue Mar 25 09:13:31 2008
From: cmiller <@t> physlab.com (Cheri Miller)
Date: Tue Mar 25 09:14:02 2008
Subject: [Histonet] Reprocessing tissue
In-Reply-To: 
References: 
Message-ID: <002101c88e82$67756a60$3d02a8c0@plab.local>

And thank you Delores for saying it!!

Cheryl Miller HT (ASCP)
Histology Supervisor
Physicians Laboratory,P.C.
Omaha, Ne. 
402 738 5052
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dolores
Townsend
Sent: Tuesday, March 25, 2008 7:35 AM
To: kemlo; 'Joe Nocito'; 'Cheryl'
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Reprocessing tissue

Well, actually...
Somewhere, somehow, a Scientist who was also a Histology Technician was
reprocessing tissue the way you do, through xylene and alcohol, when
(s)he looked at the cleaning cycle on the processor: xylene, than
alcohol. And (s)he thought: "Mmmm, I wonder..."
So (s)he tried it and it worked.
Than another Scientist, who happened to be a Histology Technician,
thought: "Is it really necessary to clear the tissue before reprocessing
it?"
And (s)he tried, and it worked. So they wrote a paper about it to let
everyone know...
No, Science is not dead. Someone had to come up with the automated
processor, and the vacuum, and the microwave staining and processing. I
am sure that the future will still show new improvements. But it's going
to be very hard to embed or cut or troubleshoot the staining without a
Histology Technician, who also has to be a Scientist.
Dolores
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message.  If
you are not the addressee intended / indicated or agent responsible for
delivering it to the addressee, you are hereby notified that you are in
possession of confidential and privileged information.  Any dissemination,
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received this message in error, please notify the sender immediately and
delete this email from your system.



PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message.  If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information.  Any dissemination, distribution, or copying of this e-mail is strictly prohibited.  If you have received this message in error, please notify the sender immediately and delete this email from your system.



From anh2006 <@t> med.cornell.edu  Tue Mar 25 10:15:46 2008
From: anh2006 <@t> med.cornell.edu (Andrea Hooper)
Date: Tue Mar 25 10:16:02 2008
Subject: [Histonet] embedding problem small zebrafish
Message-ID: 

Have you checked out the histological methods section of "The Zebrafish Book" by Monte Westerfield, on zfin.org available online here:
http://zfin.org/zf_info/zfbook/cont.html#cont8
I don't think it has paraffin embedding protocols but perhaps there are some other hints will help? In any case, it's an amazing resource.

I am also wondering if it might help to embed in agarose before paraffin embedding - especially those 44 hour old critters who are tiny tiny tiny. Unfortunately most of my experience in that regard lies with frozen sectioning so I don't have too much to add.


----- Original Message -----
From: Barbara Verstraeten 
Date: Tuesday, March 25, 2008 3:50 am
Subject: [Histonet] embedding problem small zebrafish

> Hello,
> 
> I work on zebrafish from 44 hours to 6 days old (so they are very  
> small; max. 0.5 cm).
> I would like to perform immostainings on them (such as E-cadherin  
> staining). I've embedded my species in technovit 9100 but the 
> cutting  
> of such small animals is very difficult.
> I did some stainings already and the antibody works but the problem 
> is  
> that my tissue is of very bad quality!! When I have 50 sections 
> only 1  
> is usefull.
> This is not very good!
> 
> Can someone give me any suggestions on how to embed them so that 
> they  
> are more easy to section and that I can still stain them with  
> different antibodies?
> 
> Thank you all in advance!
> 
> Kind regards,
> 
> Barbara Verstraeten
> VMDB
> University of Ghent
> Belgium
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 


From wilson_c <@t> ricerca.com  Tue Mar 25 11:08:09 2008
From: wilson_c <@t> ricerca.com (Wilson, Carol)
Date: Tue Mar 25 11:09:04 2008
Subject: [Histonet] Cost Analysis - Tissue processors
Message-ID: <9D443EB9D0270143B5AAF190CB1A58A306796300@dogwood.ricerca.com>

I'm looking at having to provide a cost analysis for an upcoming
purchase of possibly a VIP5 or ASP300 processor for our research lab.
Can anyone provide me some insight as to what I need to include or
possibly a template or example someone out there has previously used?
Thanks in advance for any help or input anyone can offer.  

 

Carol Wilson, HT(ASCP)

Lead Technician/Histology

Ricerca Biosciences, LLC

 

From palladineus <@t> yahoo.com  Tue Mar 25 11:13:01 2008
From: palladineus <@t> yahoo.com (Randy Khoo)
Date: Tue Mar 25 11:13:05 2008
Subject: [Histonet] Positions in Alpharetta, GA
In-Reply-To: <032420082103.13091.47E8171300061C750000332322230650029B0A02D2089B9A019C04040A0DBF089B0E9F0B019F@att.net>
Message-ID: <746641.35564.qm@web36507.mail.mud.yahoo.com>

Please unsubscribe.....I have wrote in a few times to
unsubscribe but I am still getting e-mails.

Randy



--- podpath@bellsouth.net wrote:

> Exciting job opportunity with a new reference lab in
> Alpharetta, GA. Lab will open summer 2008.
> Currently, there are two histotechnologist positions
> open. Applicants should be HTL or HT certified. Job
> description includes general histology including
> experience with manual special stains and immunos.
> 
> The lab is offering a very competitive benefits
> package and very congenial work environment. Other
> positions also available include data entry and
> pathology/medical transcription. Interested parties
> should respond with resume or inquiry to
> podpath@bellsouth.net. 
> 
> If you want to be part of something new with
> excellent growth potential and a family like
> atmosphere, then this job may be for you.
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 



      ____________________________________________________________________________________
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From cad <@t> Stowers-Institute.org  Tue Mar 25 11:26:34 2008
From: cad <@t> Stowers-Institute.org (Dickey, Coral)
Date: Tue Mar 25 11:26:49 2008
Subject: [Histonet] embedding problem small zebrafish
In-Reply-To: 
Message-ID: 

Barbara,

Check out a paper in ScienceDirect available online at www.sciencedirect.com Entitled: High-throughput zebrafish histology by Sabaliauskas et. Al.

Good Luck!

Coral Dickey
Histology Specialist I

Stowers Institute for Medical Research
1000 E. 50th Street
Kansas City,  Missouri  64110
Phone:  816-926-4305
e-mail:  cad@stowers-institute.org


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Hooper
Sent: Tuesday, March 25, 2008 10:16 AM
To: Barbara.Verstraeten@UGent.be
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] embedding problem small zebrafish


Have you checked out the histological methods section of "The Zebrafish Book" by Monte Westerfield, on zfin.org available online here: http://zfin.org/zf_info/zfbook/cont.html#cont8
I don't think it has paraffin embedding protocols but perhaps there are some other hints will help? In any case, it's an amazing resource.

I am also wondering if it might help to embed in agarose before paraffin embedding - especially those 44 hour old critters who are tiny tiny tiny. Unfortunately most of my experience in that regard lies with frozen sectioning so I don't have too much to add.


----- Original Message -----
From: Barbara Verstraeten 
Date: Tuesday, March 25, 2008 3:50 am
Subject: [Histonet] embedding problem small zebrafish

> Hello,
>
> I work on zebrafish from 44 hours to 6 days old (so they are very
> small; max. 0.5 cm). I would like to perform immostainings on them
> (such as E-cadherin staining). I've embedded my species in technovit
> 9100 but the cutting
> of such small animals is very difficult.
> I did some stainings already and the antibody works but the problem
> is
> that my tissue is of very bad quality!! When I have 50 sections
> only 1
> is usefull.
> This is not very good!
>
> Can someone give me any suggestions on how to embed them so that they
> are more easy to section and that I can still stain them with
> different antibodies?
>
> Thank you all in advance!
>
> Kind regards,
>
> Barbara Verstraeten
> VMDB
> University of Ghent
> Belgium
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From jcline <@t> wchsys.org  Tue Mar 25 11:32:50 2008
From: jcline <@t> wchsys.org (Joyce Cline)
Date: Tue Mar 25 11:33:00 2008
Subject: [Histonet] re: belair
In-Reply-To: <40026EDDE64CDA47AB382C52619ACD3C06612DCA@pmc-rfd-mx01.intranet.osfnet.org>
Message-ID: <000401c88e95$de3bc730$1d2a14ac@wchsys.org>

Belair Instrument Company, Inc
36 Commerce St.
Springfield, NJ 07081
973-912-8900
800-783-9424

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Renko,
Heather D.
Sent: Tuesday, March 25, 2008 9:48 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] re: belair

Anyone have the right spelling and or website for Belair?

Heather D. Renko, Histology Coordinator
OSF Saint Anthony Medical Center
5666 East State Street
Rockford, Illinois 61108
Direct: 815-395-5410
Heather.D.Renko@osfhealthcare.org


========================================================================
======
The information in this message is confidential and may be legally
privileged. Access to this message by anyone other than the addressee is
not authorized. If you are not the intended recipient, or an agent of
the intended recipient, any disclosure, copying, or distribution of the
message or any action or omission taken by you in reliance on it, is
prohibited and may be unlawful. If you have received this message in
error, please contact the sender immediately and permanently delete the
original e-mail, attachment(s), and any copies.
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======
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From joycefr <@t> frontiernet.net  Tue Mar 25 11:36:07 2008
From: joycefr <@t> frontiernet.net (Joyce Friedland)
Date: Tue Mar 25 11:33:21 2008
Subject: [Histonet] Recyclers
Message-ID: <5D0E1308-92C5-4BE3-8B8F-1B771F305F67@frontiernet.net>

Hi All,
Our lab is considering the purchase of a recycler for solvent, ETOH  
and formalin. Our current BR unit is at least 15 years old and many  
parts are no longer available so we want to be proactive and replace  
it before the inevitable crash. We're thinking 5 gallon capacity and  
wonder if any of you have pro or con experience with BR or CBG  
Biotech units.
Joyce Friedland
Muhlbauer Lab.

From mwich <@t> 7thwavelabs.com  Tue Mar 25 11:41:23 2008
From: mwich <@t> 7thwavelabs.com (Michele Wich)
Date: Tue Mar 25 11:41:32 2008
Subject: [Histonet] iNOS antibody
Message-ID: <2264717ADC396742A0FF0AAB674F9A0D486C01@7THWAVE-SERVER.7thwave.local>

I am looking for an iNOS (NOS II only) antibody that works on FFPE rat
tissue. Can anyone recommend one? 

Any information on this is greatly appreciated!

 


This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure.  If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited.  Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer
From jcline <@t> wchsys.org  Tue Mar 25 11:41:50 2008
From: jcline <@t> wchsys.org (Joyce Cline)
Date: Tue Mar 25 11:41:59 2008
Subject: [Histonet] re: purchasing new cryostats-opinions wanted
In-Reply-To: <40026EDDE64CDA47AB382C52619ACD3C0A09DE0D@pmc-rfd-mx01.intranet.osfnet.org>
Message-ID: <000001c88e97$201370d0$1d2a14ac@wchsys.org>

Leica 1850 UV just received one, set up was by sales rep and went
easily. I like the way these cryostats cut, just like their  microtomes.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Renko,
Heather D.
Sent: Tuesday, March 25, 2008 9:19 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] re: purchasing new cryostats-opinions wanted

Hello fellow Histotechs,

I am in the market to purchase two new cryostats in my 2008-2009 capital
year and wanted your thoughts on instruments, installation experiences
and overall thoughts on the products available.  Please email me
personally on your experience's with the latest and greatest brands in
the market.  

Heather D. Renko, Histology Coordinator
OSF Saint Anthony Medical Center
5666 East State Street
Rockford, Illinois 61108
Direct: 815-395-5410
Heather.D.Renko@osfhealthcare.org


========================================================================
======
The information in this message is confidential and may be legally
privileged. Access to this message by anyone other than the addressee is
not authorized. If you are not the intended recipient, or an agent of
the intended recipient, any disclosure, copying, or distribution of the
message or any action or omission taken by you in reliance on it, is
prohibited and may be unlawful. If you have received this message in
error, please contact the sender immediately and permanently delete the
original e-mail, attachment(s), and any copies.
========================================================================
======
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet


***** CONFIDENTIALITY NOTICE *****
This message contains confidential information and is intended only for
the individual named. If you are not the named addressee you should not
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immediately by e-mail if you have received this e-mail by mistake and
delete this e-mail from your system.


From SDrew <@t> uwhealth.org  Tue Mar 25 11:42:53 2008
From: SDrew <@t> uwhealth.org (Drew Sally A.)
Date: Tue Mar 25 11:43:03 2008
Subject: [Histonet] Pituitary D2 receptor
Message-ID: <3F328377AF4E23438E78234752652CE105D5269C@uwhis-xchng7.uwhis.hosp.wisc.edu>

Hello folks!  We have an neuropathologist asking us if there is an
antibody in the IHC world for 
staining  pituitary D2 receptor... ? 

Sally Ann Drew, MT(ASCP)
IHC/ISH Laboratory
University of Wisconsin Hosp. & Clinics
Madison, WI 53792
(608)265-6596

From ttroyer <@t> petersonlab.com  Tue Mar 25 12:00:15 2008
From: ttroyer <@t> petersonlab.com (Travis Troyer)
Date: Tue Mar 25 12:00:27 2008
Subject: [Histonet] p120-catenin
Message-ID: <000b01c88e99$b502cfe0$6e01010a@Peterson.local>

My pathologist discovered this antibody in an article and was wondering if we could start doing it.   I was wondering if anyone has heard of it and who would sell it.

Thanks,
Travis Troyer
Peterson Laboratory Services
From suneetimane <@t> hotmail.com  Tue Mar 25 12:22:56 2008
From: suneetimane <@t> hotmail.com (Suneeti Mane)
Date: Tue Mar 25 12:23:00 2008
Subject: [Histonet] images of blood clots
Message-ID: 


Hello
I am looking for images/pictures of real-life blood clots. We create some in the lab and need to compare them to real-life clots for surface texture and consistency/appearance. Can some direct me to a good resource please?
S. Mane
ImaRx Therapeutics.
_________________________________________________________________
Watch ?Cause Effect,? a show about real people making a real difference.  Learn more.
http://im.live.com/Messenger/IM/MTV/?source=text_watchcause
From mward <@t> wfubmc.edu  Tue Mar 25 12:26:49 2008
From: mward <@t> wfubmc.edu (Martha Ward)
Date: Tue Mar 25 12:26:58 2008
Subject: [Histonet] MLH-1,MSH-2,MSH-6
Message-ID: <61135F0455D33347B5AAE209B903A30421E90DFB@EXCHVS2.medctr.ad.wfubmc.edu>

Hello!  I have a request for these antibodies.  We are using the Bond
immuno stainer.  Does anyone have any vendor preferences they can share
with me?
 
Thanks in advance for your help.
 
Martha Ward, MT (ASCP) QIHC
Assistant Manager, Molecular Diagnostics Lab
Wake Forest University Baptist Medical Center
Medical Center Blvd.
Winston-Salem, NC 27157
336-716-2756
mward@wfubmc.edu
 
From Farnana <@t> nehealth.com  Tue Mar 25 12:51:12 2008
From: Farnana <@t> nehealth.com (Amy Farnan)
Date: Tue Mar 25 12:51:29 2008
Subject: [Histonet] I also have a Leica 1850 UV cryostat and an older 1850 as
	well. Love them both!! My older 1850 is about 5 yrs old and it
	is great!
Message-ID: <47E90350.26ED.00D9.0@nehealth.com>

I also have a Leica 1850 UV cryostat and an older 1850 as well. Love them both!! My older 1850 is about 5 yrs old and it is great!

Disclaimer: The information in this message is confidential.  If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender.

From settembr <@t> umdnj.edu  Tue Mar 25 12:51:33 2008
From: settembr <@t> umdnj.edu (Dana Settembre)
Date: Tue Mar 25 12:52:27 2008
Subject: [Histonet] MLH-1,MSH-2,MSH-6
Message-ID: 

I get mine from Cell Marque.


Dana Settembre, HT ASCP
Immunohistochemistry Lab
UMDNJ - University Hospital
Newark, NJ    USA


>>> Martha Ward  03/25/08 1:26 PM >>>
Hello!  I have a request for these antibodies.  We are using the Bond
immuno stainer.  Does anyone have any vendor preferences they can
share
with me?
 
Thanks in advance for your help.
 
Martha Ward, MT (ASCP) QIHC
Assistant Manager, Molecular Diagnostics Lab
Wake Forest University Baptist Medical Center
Medical Center Blvd.
Winston-Salem, NC 27157
336-716-2756
mward@wfubmc.edu 
 
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From Farnana <@t> nehealth.com  Tue Mar 25 12:53:34 2008
From: Farnana <@t> nehealth.com (Amy Farnan)
Date: Tue Mar 25 12:53:55 2008
Subject: [Histonet] We have 2 recyclers. One 5 gallon formasolve recycler
	from CBG and it recycles formalin, xylene and alcohol.
Message-ID: <47E903DE.26ED.00D9.0@nehealth.com>

We have 2 recyclers. One 5 gallon formasolve recycler from CBG and it recycles formalin, xylene and alcohol.
We have had for quite a few years and it works great. Very easy to use and tech support at CBG is excellent.
My other recycler is a 2.5 gallon formalin recycler this is also very easy to use and works very well.l

Disclaimer: The information in this message is confidential.  If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender.

From Shirley_PHUA <@t> hsa.gov.sg  Tue Mar 25 13:02:38 2008
From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA)
Date: Tue Mar 25 13:05:23 2008
Subject: [Histonet] Shirley Phua is out-of-office ...
Message-ID: 

I will be out of the office from  25-03-2008 to 26-03-2008.

I'll be away 25 March 2008 afternoon.

Pathologists:
I will process your requests when I return. If urgent, please forward your
email to Henry_Kyaw@hsa.gov.sg


From saby_joseph_a <@t> yahoo.com  Tue Mar 25 14:46:52 2008
From: saby_joseph_a <@t> yahoo.com (Joseph Saby)
Date: Tue Mar 25 14:46:56 2008
Subject: [Histonet] (no subject)
Message-ID: <111246.33978.qm@web33804.mail.mud.yahoo.com>

Tina-

One place to look is the Advance web site:  http://health-care-jobs.advanceweb.com/Default.aspx.

Once you've gone to the page, select lab positions.  Once this page comes up, you can click on Narrow Search By...... Specialization, and click on Histology/Pathology.  This list is pretty active, and has many east caost listings.

Good luck!

Since my labs are closing in Ann Arbor, I've been visiting that site (and others) a lot.  And I've been re-acquainting myself with this wonderful Histonet!  Feels like many long lost friends!

Joseph Saby, BA HT(ASCP)
Ann Arbor, MI


----- Original Message ----
From: TIna Hammel 
To: histonet@lists.utsouthwestern.edu
Sent: Monday, March 17, 2008 9:06:15 AM
Subject: [Histonet] (no subject)

My husband and I will be relocating to Delaware in 45 days.  I have been
a Histology Tech in the Air Force for 10 years.  My husband has been a
Histology Tech and also an EM tech for 6 years.  My husband is
certified.  If you know of any jobs in the Delaware area or Eastern
Shore area please respond.

Thank you for your time,
Tina Hammel
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From TJJ <@t> Stowers-Institute.org  Tue Mar 25 15:24:39 2008
From: TJJ <@t> Stowers-Institute.org (Johnson, Teri)
Date: Tue Mar 25 15:25:02 2008
Subject: [Histonet] Re: embedding problem small zebrafish
Message-ID: 

Barbara is using Technovit 9100 which is a methyl methacrylate resin, I'm presuming because she is not using any decalcification methods on her fish.

We have not used this for any aquatic species, but have done some work with femurs and spinal columns. The trick for getting good sections is to wet the block face with 30% alcohol before you cut it. We used a D-profile tungsten carbide knife on a retracting electronic microtome (Leica or Microm). The website for the Technovit 9100 kit (http://www.emsdiasum.com/microscopy/technical/datasheet/14655.aspx) has these instructions:

*       The blocks must be trimmed before cutting
*       Use of 16 mm hardened metal knife with D-form cutting edge or HKS-Knives. When cutting polymerized Technovit 9100 NEW blocks, 30% ethanol (cutting fluid) must be used
*       Transfer sections to super frost plus slides, mount with 50% ethanol (mounting fluid) and cover with PVC-foil (Kisol-foil)
*       Remove excess fluid with filter paper. Load the slides into a section-press

We made the press ourselves using wooden tongue depressors and a clamp. Each slide is covered with regular plastic wrap, and then they are all stacked together with the tongue depressors on the top and bottom of the stack. They are then clamped and placed in a 50 degree oven overnight.

Is this what you are currently doing?

Teri Johnson, HT(ASCP)QIHC
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64110


From jcline <@t> wchsys.org  Tue Mar 25 15:35:39 2008
From: jcline <@t> wchsys.org (Joyce Cline)
Date: Tue Mar 25 15:35:45 2008
Subject: [Histonet] Recyclers
In-Reply-To: <5D0E1308-92C5-4BE3-8B8F-1B771F305F67@frontiernet.net>
Message-ID: <000001c88eb7$c9a4f6d0$1d2a14ac@wchsys.org>

We have a CBG and have no problems with it. Alcohol comes back 97-98%
recycled. We recycle 95,100 alcohol and Xylene substitute. Use it for
the processor and stainer.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce
Friedland
Sent: Tuesday, March 25, 2008 12:36 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Recyclers

Hi All,
Our lab is considering the purchase of a recycler for solvent, ETOH  
and formalin. Our current BR unit is at least 15 years old and many  
parts are no longer available so we want to be proactive and replace  
it before the inevitable crash. We're thinking 5 gallon capacity and  
wonder if any of you have pro or con experience with BR or CBG  
Biotech units.
Joyce Friedland
Muhlbauer Lab.

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***** CONFIDENTIALITY NOTICE *****
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From kayd <@t> cytolabpathology.com  Tue Mar 25 15:58:02 2008
From: kayd <@t> cytolabpathology.com (Kay Duffy)
Date: Tue Mar 25 15:58:09 2008
Subject: [Histonet] (no subject)
Message-ID: <147174A7C9FDD344822BD4D944B5589E2E3E2F@08CPSMX.cytolabpathology.com>

Histology Tech opportunity in the North Seattle area. New lab, great
benefits and salary, excellent working environment. Contact
kayd@cytolabpathology.com

From kappeler <@t> patho.unibe.ch  Wed Mar 26 05:22:03 2008
From: kappeler <@t> patho.unibe.ch (Andi Kappeler)
Date: Wed Mar 26 05:22:28 2008
Subject: AW: [Histonet] MLH-1,MSH-2,MSH-6
In-Reply-To: <61135F0455D33347B5AAE209B903A30421E90DFB@EXCHVS2.medctr.ad.wfubmc.edu>
References: <61135F0455D33347B5AAE209B903A30421E90DFB@EXCHVS2.medctr.ad.wfubmc.edu>
Message-ID: <001301c88f2b$3c605110$17955c82@pi23>

Hi Martha

- MLH-1, clone G168-15, BD Pharmingen, CatNo 550838; HIER-Citrate pH 6.0
(1:50, H1(30).
- MSH-2, clone FE11, Calbiochem (used to be Oncogene Research), CatNo NA27;
HIER-Urea pH 9.5 (1:50, H2(20)).
- MSH-6, clone 44, BD Transduction, CatNo 610919; HIER-Citrate pH 6.0
(1:50, H1(30)).
- PMS-2, clone A16-4, BD Pharmingen, CatNo 556415; HIER-Citrate pH 6.0
(1:100, H1(30)).
Figures in brackets refer to dilutions and retrieval we used when testing
these antibodies on a Bond (with reasonable results, however optimization
still required). Hope this helps. Good luck with testing!

Andi Kappeler
Institute of Pathology, University of Bern, Switzerland 

-----Urspr?ngliche Nachricht-----
Von: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Martha
Ward
Gesendet: Dienstag, 25. M?rz 2008 18:27
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] MLH-1,MSH-2,MSH-6

Hello!  I have a request for these antibodies.  We are using the Bond immuno
stainer.  Does anyone have any vendor preferences they can share with me?
 
Thanks in advance for your help.
 
Martha Ward, MT (ASCP) QIHC
Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist
Medical Center Medical Center Blvd.
Winston-Salem, NC 27157
336-716-2756
mward@wfubmc.edu
 
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From hstevens3 <@t> mail.gatech.edu  Wed Mar 26 09:07:13 2008
From: hstevens3 <@t> mail.gatech.edu (Hazel Stevens)
Date: Wed Mar 26 09:07:19 2008
Subject: [Histonet] collagen coating
Message-ID: <002301c88f4a$b0ed4230$12c7c690$@gatech.edu>

 

Hi All,

 

I have received excellent responses from Histonet in the past so thought I
would try this out on you all. 

 

I am coating tissue culture plates with collagen and trying to demonstrate
complete coverage. Other than an antibody to collagen I , is there a simple
stain I could apply that would demonstrate coverage without dissolving the
collagen. Collagen seems to dissolve in methanol which seems to be a
component of some of the basic protein stains. Should I fix before staining?


Thanks for your help. Much appreciated.

Hazel 

 

Hazel Stevens, 

Georgia Tech,

Atlanta, Georgia 30332-0405 U.S.A.

 

From AGrobe2555 <@t> aol.com  Wed Mar 26 09:22:41 2008
From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com)
Date: Wed Mar 26 09:22:53 2008
Subject: [Histonet] Collagen coating
Message-ID: 

How about crystal violet (in ethanol)?  
Albert



**************Create a Home Theater Like the Pros. Watch the video on AOL 
Home.      
(http://home.aol.com/diy/home-improvement-eric-stromer?video=15?ncid=aolhom00030000000001)
From lab.mef <@t> smmc.org  Wed Mar 26 09:39:20 2008
From: lab.mef <@t> smmc.org (Meredith Fuller-Fedorczyk)
Date: Wed Mar 26 09:39:51 2008
Subject: [Histonet] Floaters, erroneous tissue,
	on a slide or embedded in block
Message-ID: <001801c88f4f$2d336370$240c0180@PCLAB11>

For monitoring risk management we keep a monthly tally of floaters found on stained slides. Even with cleaning of molds
and wiping down embedding area we still might have a month with two or three incidences. Is there any data for an average expectation of occurrences per month.
From HParker <@t> Skaggs.Net  Wed Mar 26 09:44:22 2008
From: HParker <@t> Skaggs.Net (Parker, Helayne)
Date: Wed Mar 26 09:44:24 2008
Subject: [Histonet] Training a new prospect
In-Reply-To: <20080324171822.F305C34C024@barracuda.skaggs.net>
Message-ID: <2FAF8CC41C5AAF43AAA52F26782E1A5602610E0E@mail1-schc.skaggs.net>

Hi Guys,
  Just wondering what texts, training aids etc one would recommend to
use for training a person w/ no experience in Histo (from scratch).  The
only path experience this person has is being a Path Secretary.  I have
trained many people before but usually they were lab techs or what have
you.


Thanks,
Helayne Parker, HT (ASCP)

From fudo <@t> ufl.edu  Wed Mar 26 09:44:59 2008
From: fudo <@t> ufl.edu (FU,DONGTAO)
Date: Wed Mar 26 09:45:04 2008
Subject: [Histonet] RNase free condition in OCT sectioning
Message-ID: <841227166.116161206542699913.JavaMail.osg@osgjas03.cns.ufl.edu>

Hi, all

  A PI want to get some sections from OCT blocks under RNase free 
condition, so they can isolate RNA from them. I will use RNaseZap 
to clean work station. What else can I do to prevent RNase 
contamination? Any suggestions will be helpful.

  Thank you,

Ann


From koellingr <@t> comcast.net  Wed Mar 26 09:52:55 2008
From: koellingr <@t> comcast.net (koellingr@comcast.net)
Date: Wed Mar 26 09:53:03 2008
Subject: [Histonet] collagen coating
Message-ID: <032620081452.32.47EA6347000320160000002022007636929D09020704040A0105@comcast.net>

depending on how good your inverted TC scope is, when I was doing a lot of tissue culture and preping some plates with collagen, you could see the coverage without stains because you can see the collagen fibers coating the well. Just have to make the light "contrasty" in the plane of view and you can see them.  
Ray Koelling
Phenopath Labs
Seattle, WA

 -------------- Original message ----------------------
From: "Hazel Stevens" 
>  
> 
> Hi All,
> 
>  
> 
> I have received excellent responses from Histonet in the past so thought I
> would try this out on you all. 
> 
>  
> 
> I am coating tissue culture plates with collagen and trying to demonstrate
> complete coverage. Other than an antibody to collagen I , is there a simple
> stain I could apply that would demonstrate coverage without dissolving the
> collagen. Collagen seems to dissolve in methanol which seems to be a
> component of some of the basic protein stains. Should I fix before staining?
> 
> 
> Thanks for your help. Much appreciated.
> 
> Hazel 
> 
>  
> 
> Hazel Stevens, 
> 
> Georgia Tech,
> 
> Atlanta, Georgia 30332-0405 U.S.A.
> 
>  
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From hfedor <@t> jhmi.edu  Wed Mar 26 10:13:06 2008
From: hfedor <@t> jhmi.edu (Helen Fedor)
Date: Wed Mar 26 10:13:29 2008
Subject: [Histonet] RNase free condition in OCT sectioning
In-Reply-To: <841227166.116161206542699913.JavaMail.osg@osgjas03.cns.ufl.edu>
References: <841227166.116161206542699913.JavaMail.osg@osgjas03.cns.ufl.edu>
Message-ID: <47EA2FC2.61A1.0088.3@jhmi.edu>

Hello Ann,
This is how I usually go about it.
Use 100% ethanol or RNase ZAP to wipe down the cryostat handle, glass door, buttons, everything that may be touched.  Then clean all tools, Brush, forceps etc. with ethanol. Place paper towels on top of the cryostat, and write out the slides with gloves on my hands on top of the paper towels. If you are going to be sectioning and collecting into tubes, put the tubes onto crushed dry ice into a small container, inside the cryostat, to keep the sections well frozen and cut down on static build-up. 
Use a new, clean blade (wiped with ethanol) for each sample to keep from contaminating your samples during sectioning.
We have excellent results for LCM and for tube methods.

Helen

>>> "FU,DONGTAO"  3/26/2008 10:44 AM >>>
Hi, all

  A PI want to get some sections from OCT blocks under RNase free 
condition, so they can isolate RNA from them. I will use RNaseZap 
to clean work station. What else can I do to prevent RNase 
contamination? Any suggestions will be helpful.

  Thank you,

Ann


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From raj <@t> bluemarble.net  Wed Mar 26 10:24:02 2008
From: raj <@t> bluemarble.net (Rebecca Johnson)
Date: Wed Mar 26 10:24:12 2008
Subject: [Histonet] Surgipath Hemotoxilyn stain setup
Message-ID: <002f01c88f55$6ca2a650$7b48f9d8@CHURCH>

Is anyone out there, that has tried Surgipath new stain setup?  Which is 
their hemo, esoin,bluing and clarifier?  We are now using Richard Allen. 
They say the hemo is crisper, and you get the 3 colors with the esoin. Also 
is suppose to be cheaper to use. Any advice is appreciated.
Thanks Becky 



From djohnson <@t> mercedesmedical.com  Wed Mar 26 10:31:31 2008
From: djohnson <@t> mercedesmedical.com (Dave Johnson)
Date: Wed Mar 26 10:31:37 2008
Subject: [Histonet] Digest
Message-ID: 



From elizabeth.heimrich <@t> bms.com  Wed Mar 26 10:32:45 2008
From: elizabeth.heimrich <@t> bms.com (Elizabeth M Heimrich)
Date: Wed Mar 26 10:32:51 2008
Subject: [Histonet] Training a new prospect
In-Reply-To: <2FAF8CC41C5AAF43AAA52F26782E1A5602610E0E@mail1-schc.skaggs.net>
References: <2FAF8CC41C5AAF43AAA52F26782E1A5602610E0E@mail1-schc.skaggs.net>
Message-ID: <47EA6C9D.1060000@bms.com>

I found Freida Carson's Histotechnology; a Self Instructional Text very 
useful.  It is easy to read and understand, and it comes with a workbook. 
Sheehan and Hrapchak's Theory aand Practice of Histotechnology is 
another wonderful resource... a little more in depth.  Have her also get 
an atlas of anatomy of what ever species you deal with most often.  Or 
even an anatomy coloring book (Kapit and Elson is the one I have).
Best of luck,
Beth

Parker, Helayne wrote:

>Hi Guys,
>  Just wondering what texts, training aids etc one would recommend to
>use for training a person w/ no experience in Histo (from scratch).  The
>only path experience this person has is being a Path Secretary.  I have
>trained many people before but usually they were lab techs or what have
>you.
>
>
>Thanks,
>Helayne Parker, HT (ASCP)
>
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>  
>
From mohs76009 <@t> yahoo.com  Wed Mar 26 11:09:49 2008
From: mohs76009 <@t> yahoo.com (Matt Bancroft)
Date: Wed Mar 26 11:10:00 2008
Subject: [Histonet] Surgipath Hemotoxilyn stain setup
In-Reply-To: <002f01c88f55$6ca2a650$7b48f9d8@CHURCH>
Message-ID: <370765.91984.qm@web63405.mail.re1.yahoo.com>

I use the hematoxylin, eosin and bluuing from surgipath.  On my clarifier I use Richard Allen.  When I was using surgipath define I was having my margin ink wash out.

Rebecca Johnson  wrote:  Is anyone out there, that has tried Surgipath new stain setup? Which is 
their hemo, esoin,bluing and clarifier? We are now using Richard Allen. 
They say the hemo is crisper, and you get the 3 colors with the esoin. Also 
is suppose to be cheaper to use. Any advice is appreciated.
Thanks Becky 



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


       
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From jcline <@t> wchsys.org  Wed Mar 26 11:35:04 2008
From: jcline <@t> wchsys.org (Joyce Cline)
Date: Wed Mar 26 11:35:10 2008
Subject: [Histonet] Surgipath Hemotoxilyn stain setup
In-Reply-To: <002f01c88f55$6ca2a650$7b48f9d8@CHURCH>
Message-ID: <000e01c88f5f$589a2020$1d2a14ac@wchsys.org>

I use the new Surgipath stains, I have received better ratings on our
H&E's from the HQUIP program since I have changed. On the Define and
Blue Buffer, you have to put those reagents in the staining bucket first
and then add the 70% alcohol and water to mix well.
The Eosin does have the three levels of staining needed.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rebecca
Johnson
Sent: Wednesday, March 26, 2008 11:24 AM
To: histonet
Subject: [Histonet] Surgipath Hemotoxilyn stain setup

Is anyone out there, that has tried Surgipath new stain setup?  Which is

their hemo, esoin,bluing and clarifier?  We are now using Richard Allen.

They say the hemo is crisper, and you get the 3 colors with the esoin.
Also 
is suppose to be cheaper to use. Any advice is appreciated.
Thanks Becky 



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


***** CONFIDENTIALITY NOTICE *****
This message contains confidential information and is intended only for
the individual named. If you are not the named addressee you should not
disseminate, distribute or copy this e-mail. Please notify the sender
immediately by e-mail if you have received this e-mail by mistake and
delete this e-mail from your system.


From Susan.Weber2 <@t> va.gov  Wed Mar 26 11:45:04 2008
From: Susan.Weber2 <@t> va.gov (Weber, Susan (VHACLE))
Date: Wed Mar 26 11:45:11 2008
Subject: [Histonet] (no subject)
Message-ID: <16C83872A53F4346AA9C3A18E3A3AAB903F76D51@VHAV10MSGA1.v10.med.va.gov>

Hello everyone! We are in the process of looking at a new dictation
system for our Lab. Currently we are looking at Powerscribe. We are
interested in hearing about similar systems and how people like this
particular system and/or how they have incorporated it into their
workflow etc.  This will change our workflow and needs careful
consideration. Currently the techs accession the specimens, our
pathologists are doing the gross through Dictaphone and our secretaries
transcribe it into Vista (our current hospital system).  The
pathologists either handwrite or dictate the micros/diagnosis for
transcription.

Thank you in advance for you advice and assistance, I find this forum to
be a wonderful resource! (Oh yes, I do also appreciate the sense of
humor sometimes displayed here too! - If I didn't have a sense of humor
I would have no sense at all!!)  :-)

 

Susan M Weber HT(ASCP)

Histology Supervisor

Louis Stokes Cleveland VA Medical Center

10701 East Blvd

Cleveland, Ohio 44106

(216) 791-3800 X6154

 

From pruegg <@t> ihctech.net  Wed Mar 26 12:07:27 2008
From: pruegg <@t> ihctech.net (Patsy Ruegg)
Date: Wed Mar 26 12:07:40 2008
Subject: [Histonet] cd markers on mouse joints
Message-ID: <005101c88f63$e3207ab0$6401a8c0@Patsyoffice>

Is anyone doing cd markers on mouse joints?

The last I heard from Gayle Callis cd markers for mouse tissue were still
being done on frozen tissues only???

Just wondering if I have missed some advances in this area.  The client is
especially interested in CD20 for B cells on mouse joints.

 

Best regards,

 

Patsy

 

 

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg@ihctech.net
web site www.ihctech.net

 


This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged & confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited. If
you are NOT the intended recipient please contact the sender and dispose of
this e-mail as soon as possible.

 

From fcmalhao <@t> icbas.up.pt  Wed Mar 26 12:10:42 2008
From: fcmalhao <@t> icbas.up.pt (=?iso-8859-1?Q?Fernanda_Malh=E3o?=)
Date: Wed Mar 26 12:11:28 2008
Subject: [Histonet] technovit
Message-ID: <001001c88f64$52d743c0$8605a8c0@porto.icbas.up.pt>

Hello everybody!
We are trying to cut a biomaterial embebed in technovit 8100, but not with success. All sections wrinkle and don?t strecth in water. Besides that when we try to stain we loose section, even using silane slides. Any suggestions will be appreciated.
Thank you all in advance.
Fernanda Malh?o
Laboratory of Histhology and Embriology
ICBAS
University of Oporto

From gvdobbin <@t> ihis.org  Wed Mar 26 12:28:17 2008
From: gvdobbin <@t> ihis.org (Greg Dobbin)
Date: Wed Mar 26 12:28:39 2008
Subject: Fwd: Re: [Histonet] Surgipath Hemotoxilyn stain setup
Message-ID: 



Greg Dobbin, R.T.
Chief Technologist, Histology Lab
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE    C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

There is some merit in doing the right thing rather badly,
but absolutely none in doing the wrong thing excellently!

>>> Greg Dobbin 3/26/2008 1:45 PM >>>
Hi Rebecca,
I am using the Selectech Reagents (all of them) on our Leica
Autostainer.  I am using Eosin Y though, not the trichrome.  I'm not
sure why Define would remove the margin ink for Matt's slides? It is
only 70% alcohol with a capful of the Define reagent. I have not heard
any complaints from my docs.  

I think they are about the same cost as we were spending previously
only now we don't need to filter the reagents every day- so time saved. 
If you are interested I have created a set of slide counting worksheets
that tells the techs when to to change the Eosin, Haematoxylin and the
alcohols and xylenes (this is for the Leica Autostainer of course).  You
start out with brand new H & E reagents and then because they are
changed at different numbers of slides (2500 for H and 1500 for E), I
have a set of 7 or 8 pages of slide counts and changes until both need
to be changed simultaneously again -then print off another set. (It will
make more sense if you see them).
Cheers.
Greg 

Greg Dobbin, R.T.
Chief Technologist, Histology Lab
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE    C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

There is some merit in doing the right thing rather badly,
but absolutely none in doing the wrong thing excellently!

>>> "Rebecca Johnson"  3/26/2008 12:24 PM >>>
Is anyone out there, that has tried Surgipath new stain setup?  Which
is 
their hemo, esoin,bluing and clarifier?  We are now using Richard
Allen. 
They say the hemo is crisper, and you get the 3 colors with the esoin.
Also 
is suppose to be cheaper to use. Any advice is appreciated.
Thanks Becky 



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From mward <@t> wfubmc.edu  Wed Mar 26 12:41:15 2008
From: mward <@t> wfubmc.edu (Martha Ward)
Date: Wed Mar 26 12:43:08 2008
Subject: [Histonet] Colloidal iron methods
Message-ID: <61135F0455D33347B5AAE209B903A30421F3FEB8@EXCHVS2.medctr.ad.wfubmc.edu>

My manager asked me to post this for him:
 
Which method are people using for colloidal iron:   Hales, Mowry's or
something else?
 
Thank you in advance for your replies.
 
Martha Ward, MT (ASCP) QIHC
Assistant Manager, Molecular Diagnostics Lab
Wake Forest University Baptist Medical Center
Medical Center Blvd.
Winston-Salem, NC 27157
336-716-2756
mward@wfubmc.edu
 
From scoop <@t> mail.nih.gov  Wed Mar 26 13:02:56 2008
From: scoop <@t> mail.nih.gov (scoop@mail.nih.gov)
Date: Wed Mar 26 13:03:09 2008
Subject: [Histonet] question about immunofluorescence staining in 
	mouse spleen
In-Reply-To: 
References: 
	
Message-ID: 

Dear All,

Thanks to everyone who responded to my question about 
immunofluorescence staining in mouse spleen and elimination of 
background.  The specific protocols and the info about macrophages 
taking up fluorescent dye nonspecifically will be very helpful.  I 
relayed the info (the question was for someone else's project) and I 
think the suggestions will be tried.  Also, I plan to start trying 
some stuff on mouse spleen myself - I've always avoided it and used 
bone marrow but your suggestions make it seem feasible.

Thanks,
Sharon

Sharon

>Hi Sharon,
>
>For immunofluorescence, frozen sections are the easiest way to go 
>with the best results.
>
>Acetone, methanol or a even a mix of acetone:ethanol are wonderful 
>ways to fix frozen sections of murine spleen for IF. A brief 2-4% 
>PFA fixation on fresh frozen splenic sections works also but the PFA 
>will increase the "autofluorescence". It will depend on the antibody 
>which fixation works best ...
>
>Do you have access to a confocal or similar type system (one with 
>apotome or deconvolution software etc.)? If so, you can do 30-100 um 
>sections with beautiful results. For regular epifluorescence, we use 
>8-12 um sections with excellent results.
>
>Let me know if you need further information/specifics,
>Andrea
>
>>Dear experts,
>>
>>My boss has asked me about immunofluorescence staining in mouse 
>>spleen:  How do you get rid of fluorescent background in mouse 
>>spleen?  I suggested ammonia ethanol quenching which I know works 
>>in bone marrow.  Is there a better method?  Also, my boss claims 
>>that the macrophages have autofluorescence (formalin fixed paraffin 
>>embedded tissue).  I always thought the rbcs would be the 
>>autofluorescent cells.  Do the macrophages have autofluorescence? 
>>Finally, is it reasonable to do frozen sections of mouse spleen?  I 
>>thought frozen sections fixed in ice cold acetone or 
>>acetone-methanol might solve the problem, but I was told that 
>>frozen sections would be much to thick to get any good 
>>immunofluorescent pictures on.
>>
>>Thanks for any info and suggestions.
>>
>>Unknowledgeably yours,
>>Sharon
>
>--
>
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From tjasper <@t> copc.net  Wed Mar 26 13:09:55 2008
From: tjasper <@t> copc.net (Thomas Jasper)
Date: Wed Mar 26 13:10:02 2008
Subject: [Histonet] Training a new prospect
References: <2FAF8CC41C5AAF43AAA52F26782E1A5602610E0E@mail1-schc.skaggs.net>
Message-ID: <90354A475B420441B2A0396E5008D4965E2091@copc-sbs.COPC.local>

Wow Helayne, sounds like quite a challenge.  Not to be unfair, as I
don't know the individual you're training, but that's a tall order.  So
many people attend college for at least 2 years to train.  And the OJT
path is not available for certification.  I do imagine it is helpful
having Path Secretary experience.  The terminology should have a good
foundation in any case.
As far as texts and training aids are concerned...I would recommend the
Theory and Practice of Histotechnology by Sheehan and Hrapchak, commonly
referred to as "Sheehan" by a lot of folks (no slight to Hrapchak
intended).  Also, Theory and Practice of Histological Techniques by
Bancroft and Stevens.  These 2 publications have been of solid service
to me over the years.  If you can get your hands on an AFIP manual, I'd
do it.  I don't have one, but have had access off and on in different
positions I've held, it's a great reference.  I also like the Atlas of
Human Histology by diFiore, it's a very nice illustrated reference.  I
would also recommend a good Anatomy and Physiology text of some sort as
well as a basic chemistry text (at least to have on hand to refer to).
I know other techs who've used Preece (spelling?).  I've heard that's a
good one, I've seen it but do not own a copy.  I know there are other
good reference out there.  I can't remember the names as I don't own the
books.
I think the NSH is a valuable resource as well.  They publish study
guides for taking the certification exam and I'm sure have recommended
texts as well.  Any state conventions/workshops could be valuable too.
Many seminars are geared at the basic level and probably would not
overwhelm this person.  A basic understanding of IHC would be helpful,
but that may be something to consider down the road.  Having access to
the histonet is of great value as well.  I'm certain you'll get some
great recommendations from the knowledgeable folks on this list.
Good luck,

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, OR 97701
541/693-2677

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Parker,
Helayne
Sent: Wednesday, March 26, 2008 7:44 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Training a new prospect

Hi Guys,
  Just wondering what texts, training aids etc one would recommend to
use for training a person w/ no experience in Histo (from scratch).  The
only path experience this person has is being a Path Secretary.  I have
trained many people before but usually they were lab techs or what have
you.


Thanks,
Helayne Parker, HT (ASCP)

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From beverly <@t> histologytechservices.com  Wed Mar 26 13:36:51 2008
From: beverly <@t> histologytechservices.com (Beverly Robinson)
Date: Wed Mar 26 13:29:07 2008
Subject: [Histonet] Desiccated slide boxes
Message-ID: <0MKp8S-1JeaMs3gEJ-0007zB@mrelay.perfora.net>

Does anyone prepare desiccated slide boxes? What is the procedure? These
slide boxes will be used to store frozen sections in a -80 C freezer. 
 
Beverly Robinson
Laboratory Manager
Histology Tech Services, Inc.
Phone: 352-338-0045
FAX: 352-338-0027
 
This email and its attachments (if any) contain confidential and privileged
information.  Any dissemination or use of this information by a person other
than the intended recipient is unauthorized and may be illegal.
 
From b-frederick <@t> northwestern.edu  Wed Mar 26 13:42:05 2008
From: b-frederick <@t> northwestern.edu (Bernice Frederick)
Date: Wed Mar 26 13:42:24 2008
Subject: [Histonet] Training a new prospect
In-Reply-To: <90354A475B420441B2A0396E5008D4965E2091@copc-sbs.COPC.local>
Message-ID: <000801c88f71$1a576270$d00f7ca5@lurie.northwestern.edu>

Helayne,
Have you pulled the requirements from the web site for certification? Sounds
like your path secretary may need to go back to school.
Bernice

Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas
Jasper
Sent: Wednesday, March 26, 2008 1:10 PM
To: Parker, Helayne
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Training a new prospect

Wow Helayne, sounds like quite a challenge.  Not to be unfair, as I
don't know the individual you're training, but that's a tall order.  So
many people attend college for at least 2 years to train.  And the OJT
path is not available for certification.  I do imagine it is helpful
having Path Secretary experience.  The terminology should have a good
foundation in any case.
As far as texts and training aids are concerned...I would recommend the
Theory and Practice of Histotechnology by Sheehan and Hrapchak, commonly
referred to as "Sheehan" by a lot of folks (no slight to Hrapchak
intended).  Also, Theory and Practice of Histological Techniques by
Bancroft and Stevens.  These 2 publications have been of solid service
to me over the years.  If you can get your hands on an AFIP manual, I'd
do it.  I don't have one, but have had access off and on in different
positions I've held, it's a great reference.  I also like the Atlas of
Human Histology by diFiore, it's a very nice illustrated reference.  I
would also recommend a good Anatomy and Physiology text of some sort as
well as a basic chemistry text (at least to have on hand to refer to).
I know other techs who've used Preece (spelling?).  I've heard that's a
good one, I've seen it but do not own a copy.  I know there are other
good reference out there.  I can't remember the names as I don't own the
books.
I think the NSH is a valuable resource as well.  They publish study
guides for taking the certification exam and I'm sure have recommended
texts as well.  Any state conventions/workshops could be valuable too.
Many seminars are geared at the basic level and probably would not
overwhelm this person.  A basic understanding of IHC would be helpful,
but that may be something to consider down the road.  Having access to
the histonet is of great value as well.  I'm certain you'll get some
great recommendations from the knowledgeable folks on this list.
Good luck,

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, OR 97701
541/693-2677

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Parker,
Helayne
Sent: Wednesday, March 26, 2008 7:44 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Training a new prospect

Hi Guys,
  Just wondering what texts, training aids etc one would recommend to
use for training a person w/ no experience in Histo (from scratch).  The
only path experience this person has is being a Path Secretary.  I have
trained many people before but usually they were lab techs or what have
you.


Thanks,
Helayne Parker, HT (ASCP)

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From Traczyk7 <@t> aol.com  Wed Mar 26 14:34:57 2008
From: Traczyk7 <@t> aol.com (Traczyk7@aol.com)
Date: Wed Mar 26 14:35:12 2008
Subject: [Histonet] Workload Recording
Message-ID: 

Will someone please direct me to a source for histology workload  recording?  
I did not find it on the ASCP website but I suspect I'm not  looking in the 
right place.  I remember using a list where each task  was assigned a code and 
weight.  Is it still used?
Thanks,
Dorothy 



**************Create a Home Theater Like the Pros. Watch the video on AOL 
Home.      
(http://home.aol.com/diy/home-improvement-eric-stromer?video=15?ncid=aolhom00030000000001)
From mpence <@t> grhs.net  Wed Mar 26 15:39:48 2008
From: mpence <@t> grhs.net (Mike Pence)
Date: Wed Mar 26 15:39:52 2008
Subject: [Histonet] Thermo Fisher Shandon
Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3791@IS-E2K3.grhs.net>

Just inquiring to ask all on the net if anyone would have a Master
circuit board squirreled away some where for a HistoCentre II from
Thermo/Shandon.
 
I don't understand why companies stop making parts for instruments that
are less than 10 years old (yes I do understand ,$$$$$). We have become
a disposable people.
 
Mike
From Paul <@t> Firnschild.com  Wed Mar 26 20:21:23 2008
From: Paul <@t> Firnschild.com (Paul Firnschild)
Date: Wed Mar 26 19:21:28 2008
Subject: [Histonet] Thermo Fisher Shandon
References: <661949901A768E4F9CC16D8AF8F2838C017A3791@IS-E2K3.grhs.net>
Message-ID: <022c01c88fa8$df248790$cf977e18@PhelpsDodge>

Mike,

I operate a repair business in the Atlanta area and I share your
frustration.  The art of circuit board repair has all but disapeared.
Almost but not quite.  We have had good results in troubleshooting circuit
boards.  Let me know if you want to send it in.  We won't charge you if we
can't repair it.

Paul

Paul M. Firnschild
QA Support Services, Inc.
404.291.3715 (t)
419.818.3618 (f)
email: Paul@Firnschild.com



----- Original Message ----- 
From: "Mike Pence" 
To: 
Sent: Wednesday, March 26, 2008 3:39 PM
Subject: [Histonet] Thermo Fisher Shandon


Just inquiring to ask all on the net if anyone would have a Master
circuit board squirreled away some where for a HistoCentre II from
Thermo/Shandon.

I don't understand why companies stop making parts for instruments that
are less than 10 years old (yes I do understand ,$$$$$). We have become
a disposable people.

Mike
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From stern <@t> ipmc.cnrs.fr  Thu Mar 27 06:20:02 2008
From: stern <@t> ipmc.cnrs.fr (Alejandro Ortiz Stern)
Date: Thu Mar 27 06:20:06 2008
Subject: [Histonet] Unsusbscribe
In-Reply-To: <022c01c88fa8$df248790$cf977e18@PhelpsDodge>
References: <661949901A768E4F9CC16D8AF8F2838C017A3791@IS-E2K3.grhs.net>
	<022c01c88fa8$df248790$cf977e18@PhelpsDodge>
Message-ID: <47EB82E2.2020600@ipmc.cnrs.fr>

This is the third mail I write asking to be unsubscribed.

Please unsubscribe me.

From rjbuesa <@t> yahoo.com  Thu Mar 27 07:30:35 2008
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Thu Mar 27 07:30:44 2008
Subject: [Histonet] Floaters, erroneous tissue,
	on a slide or embedded in block
In-Reply-To: <001801c88f4f$2d336370$240c0180@PCLAB11>
Message-ID: <72660.52180.qm@web65706.mail.ac4.yahoo.com>

If you do what is really necessary regarding cleaning of the embedding center wells and all other steps, your expectations should be "0" floaters. 
  Ren? J.

Meredith Fuller-Fedorczyk  wrote:
  For monitoring risk management we keep a monthly tally of floaters found on stained slides. Even with cleaning of molds
and wiping down embedding area we still might have a month with two or three incidences. Is there any data for an average expectation of occurrences per month.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


       
---------------------------------
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From rjbuesa <@t> yahoo.com  Thu Mar 27 07:32:28 2008
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Thu Mar 27 07:32:34 2008
Subject: [Histonet] Colloidal iron methods
In-Reply-To: <61135F0455D33347B5AAE209B903A30421F3FEB8@EXCHVS2.medctr.ad.wfubmc.edu>
Message-ID: <500985.91100.qm@web65705.mail.ac4.yahoo.com>

Mowry's
  Ren? J.

Martha Ward  wrote:
  My manager asked me to post this for him:

Which method are people using for colloidal iron: Hales, Mowry's or
something else?

Thank you in advance for your replies.

Martha Ward, MT (ASCP) QIHC
Assistant Manager, Molecular Diagnostics Lab
Wake Forest University Baptist Medical Center
Medical Center Blvd.
Winston-Salem, NC 27157
336-716-2756
mward@wfubmc.edu

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


       
---------------------------------
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From cmiller <@t> physlab.com  Thu Mar 27 07:41:11 2008
From: cmiller <@t> physlab.com (Cheri Miller)
Date: Thu Mar 27 07:41:57 2008
Subject: [Histonet] Colloidal iron methods
In-Reply-To: <61135F0455D33347B5AAE209B903A30421F3FEB8@EXCHVS2.medctr.ad.wfubmc.edu>
References: <61135F0455D33347B5AAE209B903A30421F3FEB8@EXCHVS2.medctr.ad.wfubmc.edu>
Message-ID: <000f01c89007$d68ec230$3d02a8c0@plab.local>

Mowry's

Cheryl Miller HT (ASCP)
Histology Supervisor
Physicians Laboratory,P.C.
Omaha, Ne. 
402 738 5052

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward
Sent: Wednesday, March 26, 2008 12:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Colloidal iron methods

My manager asked me to post this for him:
 
Which method are people using for colloidal iron:   Hales, Mowry's or
something else?
 
Thank you in advance for your replies.
 
Martha Ward, MT (ASCP) QIHC
Assistant Manager, Molecular Diagnostics Lab
Wake Forest University Baptist Medical Center
Medical Center Blvd.
Winston-Salem, NC 27157
336-716-2756
mward@wfubmc.edu
 
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message.  If
you are not the addressee intended / indicated or agent responsible for
delivering it to the addressee, you are hereby notified that you are in
possession of confidential and privileged information.  Any dissemination,
distribution, or copying of this e-mail is strictly prohibited.  If you have
received this message in error, please notify the sender immediately and
delete this email from your system.



PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message.  If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information.  Any dissemination, distribution, or copying of this e-mail is strictly prohibited.  If you have received this message in error, please notify the sender immediately and delete this email from your system.



From cmiller <@t> physlab.com  Thu Mar 27 07:48:37 2008
From: cmiller <@t> physlab.com (Cheri Miller)
Date: Thu Mar 27 07:49:19 2008
Subject: [Histonet] Surgipath Hemotoxilyn stain setup
In-Reply-To: <002f01c88f55$6ca2a650$7b48f9d8@CHURCH>
References: <002f01c88f55$6ca2a650$7b48f9d8@CHURCH>
Message-ID: <001001c89008$e0636e90$3d02a8c0@plab.local>

I used both the hematoxilyn and eosin. I liked it but my paths did not so
I'm back to Richard Allen and I like that as well.

Cheryl Miller HT (ASCP)
Histology Supervisor
Physicians Laboratory,P.C.
Omaha, Ne. 
402 738 5052

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rebecca
Johnson
Sent: Wednesday, March 26, 2008 10:24 AM
To: histonet
Subject: [Histonet] Surgipath Hemotoxilyn stain setup

Is anyone out there, that has tried Surgipath new stain setup?  Which is 
their hemo, esoin,bluing and clarifier?  We are now using Richard Allen. 
They say the hemo is crisper, and you get the 3 colors with the esoin. Also 
is suppose to be cheaper to use. Any advice is appreciated.
Thanks Becky 



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message.  If
you are not the addressee intended / indicated or agent responsible for
delivering it to the addressee, you are hereby notified that you are in
possession of confidential and privileged information.  Any dissemination,
distribution, or copying of this e-mail is strictly prohibited.  If you have
received this message in error, please notify the sender immediately and
delete this email from your system.



PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message.  If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information.  Any dissemination, distribution, or copying of this e-mail is strictly prohibited.  If you have received this message in error, please notify the sender immediately and delete this email from your system.



From dmccaig <@t> ckha.on.ca  Thu Mar 27 07:51:09 2008
From: dmccaig <@t> ckha.on.ca (Diana McCaig)
Date: Thu Mar 27 07:51:15 2008
Subject: [Histonet] acid washing glassware
Message-ID: 

Currently we are using Nitric/HCl acid to clean our glassware.  
Is there a product that could be substituted for this that is less
toxic?
diana
From ian.montgomery <@t> bio.gla.ac.uk  Thu Mar 27 08:16:11 2008
From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery)
Date: Thu Mar 27 08:16:25 2008
Subject: [Histonet] Barley roots
Message-ID: <002601c8900c$ba0339c0$6424d182@IBLS.GLA.AC.UK>

            A question for the botanical histologist. I've just fixed,
processed and cut barley roots for histology, so next is the staining for
general structure. Being a humble physiologist it's back to the books for
techniques. I've got a copy of Plant Microtechnique and Microscopy by Steven
Ruzin and several techniques look good. Johansen's safranin and fast green
or safranin O and orange G seems appropriate, but am I on the right track
and which one? Comments on botanic staining would be welcome.

Ian.

     

 

Dr. Ian Montgomery,

Histotechnology,

I.B.L.S. Support Unit,

Thomson Building,

University of Glasgow,

Glasgow,

G12 8QQ.

 

From Barry.R.Rittman <@t> uth.tmc.edu  Thu Mar 27 08:30:15 2008
From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R)
Date: Thu Mar 27 08:30:20 2008
Subject: [Histonet] Barley roots
In-Reply-To: <002601c8900c$ba0339c0$6424d182@IBLS.GLA.AC.UK>
References: <002601c8900c$ba0339c0$6424d182@IBLS.GLA.AC.UK>
Message-ID: 

Ian
I am not a plant physiologist  but I have processed onion roots and also
occasional some cactus and stain depends on what you want to see.
For mitoses I used Heidenhain's iron hematoxylin or Feulgen reaction and
these were really nice.
Have also used crystal violet alone or followed by Lugol's
iodine-potassium iodide.
Barry

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian
Montgomery
Sent: Thursday, March 27, 2008 8:16 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Barley roots


            A question for the botanical histologist. I've just fixed,
processed and cut barley roots for histology, so next is the staining
for
general structure. Being a humble physiologist it's back to the books
for
techniques. I've got a copy of Plant Microtechnique and Microscopy by
Steven
Ruzin and several techniques look good. Johansen's safranin and fast
green
or safranin O and orange G seems appropriate, but am I on the right
track
and which one? Comments on botanic staining would be welcome.

Ian.

     

 

Dr. Ian Montgomery,

Histotechnology,

I.B.L.S. Support Unit,

Thomson Building,

University of Glasgow,

Glasgow,

G12 8QQ.

 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
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From emerald_lake77 <@t> yahoo.com  Thu Mar 27 09:02:54 2008
From: emerald_lake77 <@t> yahoo.com (GT Hebert)
Date: Thu Mar 27 09:02:58 2008
Subject: [Histonet] Caspase-3 Antibody; other Abs -- Apoptosis vs. Necrosis
Message-ID: <375334.33077.qm@web31703.mail.mud.yahoo.com>

Hello all,
   
  Does anyone know if Caspase-3 antibody is suitable for detecting apoptotic cells (as opposed to both apoptotic and necrotic cells)?
   
  If it cannot distiguish between cells types, is there an antibody (for mouse tissue) that can be used or is my only option in situ techniques?
   
  Thank you.
   
  Gustave Hebert
  Scientist II
  Wyeth Research
  Cambridge MA

       
---------------------------------
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From rjbuesa <@t> yahoo.com  Thu Mar 27 09:04:19 2008
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Thu Mar 27 09:04:28 2008
Subject: [Histonet] Barley roots
In-Reply-To: 
Message-ID: <992108.79024.qm@web65705.mail.ac4.yahoo.com>

  Ian:
  For plant materials )like leaves, stems, etc) I always used light green and safranin with very good results.
  Ren? J.
  

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian
Montgomery
Sent: Thursday, March 27, 2008 8:16 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Barley roots


A question for the botanical histologist. I've just fixed,
processed and cut barley roots for histology, so next is the staining
for
general structure. Being a humble physiologist it's back to the books
for
techniques. I've got a copy of Plant Microtechnique and Microscopy by
Steven
Ruzin and several techniques look good. Johansen's safranin and fast
green
or safranin O and orange G seems appropriate, but am I on the right
track
and which one? Comments on botanic staining would be welcome.

Ian.





Dr. Ian Montgomery,

Histotechnology,

I.B.L.S. Support Unit,

Thomson Building,

University of Glasgow,

Glasgow,

G12 8QQ.



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From rjbuesa <@t> yahoo.com  Thu Mar 27 09:16:04 2008
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Thu Mar 27 09:16:08 2008
Subject: [Histonet] acid washing glassware
In-Reply-To: 
Message-ID: <638488.88038.qm@web65709.mail.ac4.yahoo.com>

I have used CRL acid cleaners (you can buy it in any home products store, like Home Depot or Low's)
Ren? J.
  

Diana McCaig  wrote:
  Currently we are using Nitric/HCl acid to clean our glassware. 
Is there a product that could be substituted for this that is less
toxic?
diana
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From gvdobbin <@t> ihis.org  Thu Mar 27 09:31:53 2008
From: gvdobbin <@t> ihis.org (Greg Dobbin)
Date: Thu Mar 27 09:32:26 2008
Subject: [Histonet] acid washing glassware
Message-ID: 

I think you mean CLR.
Greg

Greg Dobbin, R.T.
Chief Technologist, Histology Lab
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE    C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

There is some merit in doing the right thing rather badly,
but absolutely none in doing the wrong thing excellently!

>>> Rene J Buesa  3/27/2008 11:16 AM >>>
I have used CRL acid cleaners (you can buy it in any home products store, like Home Depot or Low's)
Ren? J.
  

Diana McCaig  wrote:
  Currently we are using Nitric/HCl acid to clean our glassware. 
Is there a product that could be substituted for this that is less
toxic?
diana
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From gvdobbin <@t> ihis.org  Thu Mar 27 09:40:01 2008
From: gvdobbin <@t> ihis.org (Greg Dobbin)
Date: Thu Mar 27 09:40:13 2008
Subject: [Histonet] Decal procedures-assessing endpoints
Message-ID: 

Hello Colleagues,
Can some of you share with me, your guidelines for determining
appropriate submersion times for sections of bone or other calcified
bits in RDO (Trade name) post-fixation but prior to processing
(obviously).  I guess it is a  subjective call by necessity!?   
Thanks,
Greg

Greg Dobbin, R.T.
Chief Technologist, Histology Lab
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE    C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

There is some merit in doing the right thing rather badly,
but absolutely none in doing the wrong thing excellently!

From jengirl1014 <@t> yahoo.com  Thu Mar 27 09:42:33 2008
From: jengirl1014 <@t> yahoo.com (Jennifer Sipes)
Date: Thu Mar 27 09:42:37 2008
Subject: [Histonet] Oil Red Staining
Message-ID: <329929.37149.qm@web62414.mail.re1.yahoo.com>

Hello all!

I had a investigator come to me this morning and ask if I've ever done Oil Red Staining for hepatic steatosis before.  I have never heard of this.  Is it easy and does someone have a protocol for it or maybe a better way of staing for hepatic steatosis if this is no longer done?

Thanks for your time gang!  You guys are the best!

Jen at Hopkins


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From ksecrest <@t> hsc.wvu.edu  Thu Mar 27 09:50:35 2008
From: ksecrest <@t> hsc.wvu.edu (Kimberly Secrest)
Date: Thu Mar 27 09:51:41 2008
Subject: [Histonet] Histo Equipment and supplies
Message-ID: <47EB7BFA.C068.0078.0@hsc.wvu.edu>

Hi everyone,
I'm looking for anyone willing to donate histology equipment (i.e. microtomes, tissue processor, embedding center, routine stainer, etc) or supplies (i.e. slides, cassettes, special stain reagents and powder dyes) for the start up of a histotechnology program?  We'd be willing to cover the cost of shipping.
Thank you in advance!
Kim



Kimberly Secrest, HTL, QIHC
Instructor
Department of Pathology
West Virginia University
304-293-7628



From rjbuesa <@t> yahoo.com  Thu Mar 27 09:54:37 2008
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Thu Mar 27 09:54:40 2008
Subject: [Histonet] acid washing glassware
In-Reply-To: 
Message-ID: <395353.81616.qm@web65714.mail.ac4.yahoo.com>

Exactly! Some dislexia here!
Ren? J.

Greg Dobbin  wrote:  I think you mean CLR.
Greg

Greg Dobbin, R.T.
Chief Technologist, Histology Lab
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

There is some merit in doing the right thing rather badly,
but absolutely none in doing the wrong thing excellently!

>>> Rene J Buesa 3/27/2008 11:16 AM >>>
I have used CRL acid cleaners (you can buy it in any home products store, like Home Depot or Low's)
Ren? J.


Diana McCaig wrote:
Currently we are using Nitric/HCl acid to clean our glassware. 
Is there a product that could be substituted for this that is less
toxic?
diana
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet 



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From peter.craven <@t> nhs.net  Thu Mar 27 09:54:28 2008
From: peter.craven <@t> nhs.net (peter.craven@nhs.net)
Date: Thu Mar 27 09:54:45 2008
Subject: [Histonet] Decal procedures-assessing endpoints
In-Reply-To: 
Message-ID: <000601c8901a$776227d0$320f030a@NHSH.SCOT.NHS.UK>

Greg 
We use a chemical end point test where we neutralise 5ml of the used decal
solution with ammonia then add an equal quantity of saturated Ammonium
Oxalate and leave for 30 mins if the solution goes cloudy we change the
solution the specimen is in and keep decalcifying. If it stays clear we
process and cut the tissue. The tissue need to be in the decalcifier for at
least overnight to give an accurate reading, works with acid decalcifying
solutions but not with EDTA. We use a litmus paper strip to identify the
acid has been neutralised.

Hope this helps 

Peter L Craven FIBMS
BMS2
Pathology Department
Raigmore Hospital
Inverness
IV2 3UJ
Tel 01463 704000 ext 4269


No trees were hurt in the sending of this email
However many electrons were severly inconvienienced


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Greg Dobbin
Sent: 27 March 2008 14:40
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Decal procedures-assessing endpoints

Hello Colleagues,
Can some of you share with me, your guidelines for determining
appropriate submersion times for sections of bone or other calcified
bits in RDO (Trade name) post-fixation but prior to processing
(obviously).  I guess it is a  subjective call by necessity!?   
Thanks,
Greg

Greg Dobbin, R.T.
Chief Technologist, Histology Lab
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE    C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

There is some merit in doing the right thing rather badly,
but absolutely none in doing the wrong thing excellently!

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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From rjbuesa <@t> yahoo.com  Thu Mar 27 09:57:49 2008
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Thu Mar 27 09:57:57 2008
Subject: [Histonet] Decal procedures-assessing endpoints
In-Reply-To: 
Message-ID: <448114.89650.qm@web65708.mail.ac4.yahoo.com>

The "softness" of the tissue fragment, how it bends or compresses, will give you the most practical way of determining the decal end point.
  Be careful with the bending to prevent breaking a piece not decalcified yet.
  Ren? J.

Greg Dobbin  wrote:
  Hello Colleagues,
Can some of you share with me, your guidelines for determining
appropriate submersion times for sections of bone or other calcified
bits in RDO (Trade name) post-fixation but prior to processing
(obviously). I guess it is a subjective call by necessity!? 
Thanks,
Greg

Greg Dobbin, R.T.
Chief Technologist, Histology Lab
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

There is some merit in doing the right thing rather badly,
but absolutely none in doing the wrong thing excellently!

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


       
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From rjbuesa <@t> yahoo.com  Thu Mar 27 10:04:58 2008
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Thu Mar 27 10:05:08 2008
Subject: [Histonet] Oil Red Staining
In-Reply-To: <329929.37149.qm@web62414.mail.re1.yahoo.com>
Message-ID: <164160.45281.qm@web65715.mail.ac4.yahoo.com>

You have 2 questions: about Oil Red O (ORO) and hepatic steatosis staining.
  The second leave it to your investigator, because it seems that is the procedure s/he desires.
  About ORO is easy but has to be done on frozen tissue since the FFPE procedure will eliminate the fats targeted by ORO (that can be found in any histotechnique book).
  Ren? J. 

Jennifer Sipes  wrote:
  Hello all!

I had a investigator come to me this morning and ask if I've ever done Oil Red Staining for hepatic steatosis before. I have never heard of this. Is it easy and does someone have a protocol for it or maybe a better way of staing for hepatic steatosis if this is no longer done?

Thanks for your time gang! You guys are the best!

Jen at Hopkins


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From jstaruk <@t> masshistology.com  Thu Mar 27 10:06:22 2008
From: jstaruk <@t> masshistology.com (jstaruk)
Date: Thu Mar 27 10:06:31 2008
Subject: [Histonet] mounting and staining celloidin-embedded whole brain
	sections
In-Reply-To: <329929.37149.qm@web62414.mail.re1.yahoo.com>
Message-ID: 

All,

 

I have 160 sections of celloidin-embedded Human brain sections cut at 50um
which now have to be mounted on glass and stained.  Is there anyone out
there who's done this before?  My main concern is how to mount the sections
to the glass and also staining methods (before or after mounting?).

 

Thank you

 

PS, for all of those who requested an AFB control block from me last week,
they were mailed out yesterday.

 

Jim

 

_____________________

     Jim Staruk

Mass Histology Service

www.masshistology.com


From jm.lapointe <@t> accellab.com  Thu Mar 27 10:23:26 2008
From: jm.lapointe <@t> accellab.com (Jean-Martin Lapointe)
Date: Thu Mar 27 10:25:07 2008
Subject: [Histonet] Oil Red Staining
References: <200803271509.m2RF9WwB007402@gateway5.lastspam.com>
Message-ID: 

Hi Jennifer,
Oil red O is a stain for lipids. It must be done on frozen sections, as processing for paraffin embedding dissolves lipids. You should use snap-frozen tissue, either fresh or formalin-fixed. I have no technical pointers (not being involved in that part of the work), but the technique is fairly common and described in many texts. The resulting stain can be a bit messy, especially with very fatty tissues. Good luck !

Jean-Martin

__________________________________
Jean-Martin Lapointe, DMV, MS, dACVP
Vice-President, Pathologie
AccelLAB Inc
1635 Lionel-Bertrand, Boisbriand
Qu?bec, Canada  J7H 1N8
tel:? 450-435-9482 ext.247
fax: 450-435-4795
jm.lapointe@accellab.com
?
?


-----Original Message-----
Message: 25
Date: Thu, 27 Mar 2008 07:42:33 -0700 (PDT)
From: Jennifer Sipes 
Subject: [Histonet] Oil Red Staining
To: Histonet 
Message-ID: <329929.37149.qm@web62414.mail.re1.yahoo.com>
Content-Type: text/plain; charset=us-ascii

Hello all!

I had a investigator come to me this morning and ask if I've ever done Oil Red Staining for hepatic steatosis before.  I have never heard of this.  Is it easy and does someone have a protocol for it or maybe a better way of staing for hepatic steatosis if this is no longer done?

Thanks for your time gang!  You guys are the best!

Jen at Hopkins


*******************************

From tracy.bergeron <@t> biogenidec.com  Thu Mar 27 10:25:42 2008
From: tracy.bergeron <@t> biogenidec.com (Tracy Bergeron)
Date: Thu Mar 27 10:26:06 2008
Subject: [Histonet] Excelsior processor in Biotech/Pharma facilities
In-Reply-To: 
Message-ID: 

Hi folks,

        We are looking into getting a new processor in our lab.  We are 
trying to decide between the VIP and the Excelsior.  Several of us are 
most familiar with the VIP, and like the ease of use and # of cassettes it 
holds, the rest of our group though familiar with the VIP like the idea of 
conserving reagents by using the Excelsior. 

That said
I am looking for information from the biotech/Pharma community on the 
Excelsior. 
Is there anyone out there using this processor outside the clinical lab?
Does it work well on rodent tissue?
What about hard tissues?
Bone
Paws (mouse and rat)

My next question is for people familiar with the earlier models of the 
Excelsior as well as the newest model. (clinical or research)
I find the software for the older model very cumbersome and not user 
friendly.
Is the software on the newer machines any easier to use for the average 
person?
Is it as easy as using the VIP, or Pathcenter? - follow the prompts sort 
of thing.
 
I would appreciate any input folks have.
Thanks.

Sincerely,
Tracy E. Bergeron, B.S., HT, HTL (ASCP)
Associate Scientist III, Pathology
Comparative Pathology Laboratory
Biogen Idec
14 Cambridge Center
Cambridge, MA 02142
Direct:  617-914-1115
Fax:  617-679-3208
From arsenn <@t> hsh.org  Thu Mar 27 10:31:23 2008
From: arsenn <@t> hsh.org (Senn, Amy R)
Date: Thu Mar 27 10:31:37 2008
Subject: [Histonet] Text for beginners
Message-ID: 

Helayne,

When I was in school (about 4-5 years ago) we used Freida Carson's
Histotechnology; a Self Instructional Text.  

 

I knew nothing about histology at that point, and this book was
extremely easy to follow and understand. :-) I believe that it taught me
the foundation of everything I know about histology.

 

We have lots of books in my lab now, but I still reference Carson's
book!

 

Good luck!

Amy

 

Parker, Helayne wrote:

 

>Hi Guys,

>  Just wondering what texts, training aids etc one would recommend to

>use for training a person w/ no experience in Histo (from scratch).
The

>only path experience this person has is being a Path Secretary.  I have

>trained many people before but usually they were lab techs or what have

>you.

> 

> 

>Thanks,

>Helayne Parker, HT (ASCP)

 

 

Amy,

Histotech, Histology Laboratory

Holy Spirit Hospital

Camp Hill

 

(717) 763-2124

Cell: (724) 494-2237

 



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From saby_joseph_a <@t> yahoo.com  Thu Mar 27 10:32:27 2008
From: saby_joseph_a <@t> yahoo.com (Joseph Saby)
Date: Thu Mar 27 10:32:31 2008
Subject: [Histonet] Colloidal iron methods
Message-ID: <921329.94723.qm@web33806.mail.mud.yahoo.com>

Martha-
 
Years past I made and used both Hales and Mowry's, but ended up going over to alcian blue.  The alcian blue solution is very easy to make, but you need to adjust the pH to what you are intending to stain (3.0 for most mucopolysaccharides, 1.0 for chondroitin sulfates in cartilage).
 
Also, I had variable results until I located a good source for the powder.  Every old container you might find will stain differently.  I suggest buying some fresh from a reputable supplier, and keeping with that lot number once its usefulness has been established.

Good luck!

Joe Saby, BA HT



----- Original Message ----
From: Martha Ward 
To: histonet@lists.utsouthwestern.edu
Sent: Wednesday, March 26, 2008 1:41:15 PM
Subject: [Histonet] Colloidal iron methods

My manager asked me to post this for him:

Which method are people using for colloidal iron:  Hales, Mowry's or
something else?

Thank you in advance for your replies.

Martha Ward, MT (ASCP) QIHC
Assistant Manager, Molecular Diagnostics Lab
Wake Forest University Baptist Medical Center
Medical Center Blvd.
Winston-Salem, NC 27157
336-716-2756
mward@wfubmc.edu

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From rjbuesa <@t> yahoo.com  Thu Mar 27 11:07:52 2008
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Thu Mar 27 11:07:56 2008
Subject: [Histonet] Excelsior processor in Biotech/Pharma facilities
In-Reply-To: 
Message-ID: <755293.4240.qm@web65716.mail.ac4.yahoo.com>

Tracy:
  Do you mean the Xpress by Sakura (the same manufacturer as the VIP)?
If that is the case the Xpress is a mixed technology between "conventional" paraffin infiltration (2 retorts) and microwave technology (another 2 retorts for dehydration and clearing). It uses less reagents, but can process 40 cassettes only each time finished every 15 minutes after a 45 minutes wait. It costs $250,000
  I would never use any other tissue processor than a VIP
Ren? J.
   
   
  
Tracy Bergeron  wrote:
  Hi folks,

We are looking into getting a new processor in our lab. We are 
trying to decide between the VIP and the Excelsior. Several of us are 
most familiar with the VIP, and like the ease of use and # of cassettes it 
holds, the rest of our group though familiar with the VIP like the idea of 
conserving reagents by using the Excelsior. 

That said
I am looking for information from the biotech/Pharma community on the 
Excelsior. 
Is there anyone out there using this processor outside the clinical lab?
Does it work well on rodent tissue?
What about hard tissues?
Bone
Paws (mouse and rat)

My next question is for people familiar with the earlier models of the 
Excelsior as well as the newest model. (clinical or research)
I find the software for the older model very cumbersome and not user 
friendly.
Is the software on the newer machines any easier to use for the average 
person?
Is it as easy as using the VIP, or Pathcenter? - follow the prompts sort 
of thing.

I would appreciate any input folks have.
Thanks.

Sincerely,
Tracy E. Bergeron, B.S., HT, HTL (ASCP)
Associate Scientist III, Pathology
Comparative Pathology Laboratory
Biogen Idec
14 Cambridge Center
Cambridge, MA 02142
Direct: 617-914-1115
Fax: 617-679-3208
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


       
---------------------------------
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From pruegg <@t> ihctech.net  Thu Mar 27 11:18:19 2008
From: pruegg <@t> ihctech.net (patsy ruegg)
Date: Thu Mar 27 11:11:06 2008
Subject: [Histonet] Caspase-3 Antibody; other Abs -- Apoptosis vs. Necrosis
In-Reply-To: <375334.33077.qm@web31703.mail.mud.yahoo.com>
Message-ID: <200803271610.m2RGAvL2076490@pro12.abac.com>

In my experience CC3 does a much better job of staining just apoptotic cells
and not necrotic cells than Tunnel as a matter of fact I have never doubted
that the staining I get with CC3 is anything but apoptotic cells which is
not the case with Tunnel, we could not distinguish necrosis from apoptosis
with Tunnel.

Patsy

One complaint I get from my clients is how few cells stain with CC3, but I
think it is real and they are used to seeing more cells stain with Tunnel
because Tunnel is picking up necrotic cells as well as apoptotic cells.

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. #216
Aurora, CO 80010
720-859-4060
fax 720-859-4110
pruegg@ihctech.net
www.ihctech.net 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of GT Hebert
Sent: Thursday, March 27, 2008 8:03 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Caspase-3 Antibody; other Abs -- Apoptosis vs. Necrosis

Hello all,
   
  Does anyone know if Caspase-3 antibody is suitable for detecting apoptotic
cells (as opposed to both apoptotic and necrotic cells)?
   
  If it cannot distiguish between cells types, is there an antibody (for
mouse tissue) that can be used or is my only option in situ techniques?
   
  Thank you.
   
  Gustave Hebert
  Scientist II
  Wyeth Research
  Cambridge MA

       
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From pruegg <@t> ihctech.net  Thu Mar 27 11:28:35 2008
From: pruegg <@t> ihctech.net (patsy ruegg)
Date: Thu Mar 27 11:21:25 2008
Subject: [Histonet] mounting and staining celloidin-embedded whole
	brainsections
In-Reply-To: 
Message-ID: <200803271621.m2RGLD4r082456@pro12.abac.com>

Jim,
I used to work with celloidin 30 years ago and it was a pain.  As I recall
we used alcohol and a fine spatula and fine brush, as the section came off
the block we dropped alcohol on it and painted it onto the surface of the
large steel or tungsten blade, scooped it up onto the spatula keeping it wet
with the alcohol, then we would slide it off the spatula on to the glass
microscope slide.  I think we dryed it on a heat plate flat from there
(60-70dc) but my memory is not so clear on that.
Patsy 

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. #216
Aurora, CO 80010
720-859-4060
fax 720-859-4110
pruegg@ihctech.net
www.ihctech.net 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jstaruk
Sent: Thursday, March 27, 2008 9:06 AM
To: 'Histonet'
Subject: [Histonet] mounting and staining celloidin-embedded whole
brainsections

All,

 

I have 160 sections of celloidin-embedded Human brain sections cut at 50um
which now have to be mounted on glass and stained.  Is there anyone out
there who's done this before?  My main concern is how to mount the sections
to the glass and also staining methods (before or after mounting?).

 

Thank you

 

PS, for all of those who requested an AFB control block from me last week,
they were mailed out yesterday.

 

Jim

 

_____________________

     Jim Staruk

Mass Histology Service

www.masshistology.com


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From Barry.R.Rittman <@t> uth.tmc.edu  Thu Mar 27 11:24:28 2008
From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R)
Date: Thu Mar 27 11:24:35 2008
Subject: [Histonet] mounting and staining celloidin-embedded whole brain
	sections
In-Reply-To: 
References: <329929.37149.qm@web62414.mail.re1.yahoo.com>
	
Message-ID: 

Jim
You are now talking about real old fashioned histology!

I have cut, mounted and stained Low Viscosity Nitrocellulose sections
(of human embryos) up to 130 microns thick.
I am assuming that you are talking about LVN rather than celloidin but
the same general principles apply.

Stain, using very dilute solutions, differentiating agents etc.
Can dilute Ehrlich's hematoxylin to 1 -5 % using glass distilled water.
Then need to filter. Solution only keeps a day or so. Stain for up to
several hours. Differentiation required is usually minimal. Same
principal for eosins etc.
This is best done using free floating sections.
Sections that are stuck to slides before staining tend to have areas
that lift from the slide and give patchy staining in those areas.
These can be processed as usual through alcohols up to 70%.
90% ethanol and above will soften and then dissolve LVN and celloidin.
To prevent this go from 70% to a mixture of absolute ethanol and
chloroform.  Can use a variety of mixtures but generally need at least
15% of the mixture to be chloroform. Carful at this step not to have
excess humidity in the room or breathe too heavily on the sections.
If you use xylem or toluene then sections become brittle and curl.
I have used terpineol also known as oil of lilacin.
This will allow sections to remain supple and sections can be left in
this some several minutes.
This can be done with free floating section or can place the section on
slid and add the terpineol directly.
Terpineol is thick and so will not flow off slides and you will see a
lot of convection currently. Would recommend leaving 5-10 minutes at
least.
Carefully drain off terpineol and then remove excess terpineol remaining
on the slide using bibulous paper (or lens tissue) with several layers
of  filter paper or paper towels to absorb terpineol. 
Terpineol is miscible with most permanent mountants.
The major problem is keeping sections flat. I have used an excess of
mountant and after applying coverglass have weighted this down with
brass weights.
I had some made, if you need any please let me know as I have a half
dozen or so that I could send you.
Don't be cheapskate with the mountant or you will have bubbles creeping
in from the side.
One small point, if you need to use any enzymes on the sections then you
will have to remove the celloidin as most proteins cannot penetrate the
pores in the celloidin. Also some stains such as Celestine blue, luxol
fast blue etc. will permanently stain the celloidin and also render it
insoluble.
Hope this helps.
If you need more details please contact me.
Barry


Barry Rittman, PhD
713- 500 -4134  Office


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jstaruk
Sent: Thursday, March 27, 2008 10:06 AM
To: 'Histonet'
Subject: [Histonet] mounting and staining celloidin-embedded whole
brainsections

All,

 

I have 160 sections of celloidin-embedded Human brain sections cut at
50um
which now have to be mounted on glass and stained.  Is there anyone out
there who's done this before?  My main concern is how to mount the
sections
to the glass and also staining methods (before or after mounting?).

 

Thank you

 

PS, for all of those who requested an AFB control block from me last
week,
they were mailed out yesterday.

 

Jim

 

_____________________

     Jim Staruk

Mass Histology Service

www.masshistology.com


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From mohs76009 <@t> yahoo.com  Thu Mar 27 11:30:04 2008
From: mohs76009 <@t> yahoo.com (Matt Bancroft)
Date: Thu Mar 27 11:30:23 2008
Subject: [Histonet] Excelsior processor in Biotech/Pharma facilities
In-Reply-To: <755293.4240.qm@web65716.mail.ac4.yahoo.com>
Message-ID: <305433.5213.qm@web63408.mail.re1.yahoo.com>

If I remeber correctly the exclesior is made by Micom (thermoshandon)

Rene J Buesa  wrote:  Tracy:
Do you mean the Xpress by Sakura (the same manufacturer as the VIP)?
If that is the case the Xpress is a mixed technology between "conventional" paraffin infiltration (2 retorts) and microwave technology (another 2 retorts for dehydration and clearing). It uses less reagents, but can process 40 cassettes only each time finished every 15 minutes after a 45 minutes wait. It costs $250,000
I would never use any other tissue processor than a VIP
Ren? J.



Tracy Bergeron wrote:
Hi folks,

We are looking into getting a new processor in our lab. We are 
trying to decide between the VIP and the Excelsior. Several of us are 
most familiar with the VIP, and like the ease of use and # of cassettes it 
holds, the rest of our group though familiar with the VIP like the idea of 
conserving reagents by using the Excelsior. 

That said
I am looking for information from the biotech/Pharma community on the 
Excelsior. 
Is there anyone out there using this processor outside the clinical lab?
Does it work well on rodent tissue?
What about hard tissues?
Bone
Paws (mouse and rat)

My next question is for people familiar with the earlier models of the 
Excelsior as well as the newest model. (clinical or research)
I find the software for the older model very cumbersome and not user 
friendly.
Is the software on the newer machines any easier to use for the average 
person?
Is it as easy as using the VIP, or Pathcenter? - follow the prompts sort 
of thing.

I would appreciate any input folks have.
Thanks.

Sincerely,
Tracy E. Bergeron, B.S., HT, HTL (ASCP)
Associate Scientist III, Pathology
Comparative Pathology Laboratory
Biogen Idec
14 Cambridge Center
Cambridge, MA 02142
Direct: 617-914-1115
Fax: 617-679-3208
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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From Dorothy.L.Webb <@t> HealthPartners.Com  Thu Mar 27 11:32:07 2008
From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L)
Date: Thu Mar 27 11:32:17 2008
Subject: [Histonet] Dictation systems
Message-ID: <0E394B648E5284478A6CCB78E5AFDA27056355AE@hpes1.HealthPartners.int>

We switched last year to the "dragon" system, which is voice activated,
for the PA's and the pathologists.  The histories are put in and all of
the gross as well as the microscopic dictation.  This has been accepted
very easily by all involved and has been a dream of a system.
Obviously, it saved a lot of time and manual work so that we had to find
other positions within our facility for 2 pathology secretaries to work.

Dorothy Webb, HT (ASCP)
Histology Technical Supervisor 
Regions Hospital, Pathology Department 
640 Jackson Street, Saint Paul, MN 55101-2595 
Phone: 651-254-2962
Fax: 651-254-2741 
Regions Hospital is part of the HealthPartners family of care
________________________________________
This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited.
 
If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us.
From detmar <@t> mshri.on.ca  Thu Mar 27 12:04:16 2008
From: detmar <@t> mshri.on.ca (Jacqui Detmar)
Date: Thu Mar 27 12:04:36 2008
Subject: [Histonet] Oil Red Staining
In-Reply-To: 
References: <200803271509.m2RF9WwB007402@gateway5.lastspam.com>
	
Message-ID: 

Hi Jennifer.  As luck would have it, I just did an Oil Red stain on my
mouse placentae the other day.  The protocol I use was taken from IHC
World.  I'm often trying to determine whether a placental phenotype
exists in different mouse knockout strains, and this is one that I apply
to see how lipid metabolism is going.  Anyway, here it is:

Fix tissue in 10% formaldehyde (I don't like to use the ready-made 10%
formalin b/c I'm afraid the added methanol will affect the lipid levels.
So, I make fresh solution every time, using 37% formaldehyde and 0.1 M
phosphate buffer.

Wash in PBS.  Cryoembed in OCT.

Cut 8-10 micron sections.

Allow sections to dry at room temperature (about 15-30 minutes)

Rinse off OCT in ddH2O (about 2 changes)

Place in 100% propylene glycol for 5 minutes.

Stain in Oil Red O for 8 minutes at 60 degrees Celsius

Rinse in 85% propylene glycol for 5 minutes.

Rinse in ddH2O for 3-5 minutes.

Do *light* stain with hematoxylin (I use Harris, but the original
protocols called for Mayer's or Gill's; Also, I apply the hematoxylin
with a dropper to the section, count to 5 (Mississippi) and squirt the
stain off with water in a squirt bottle).

Blue in 1% NaHCO3

Wash in running water for 2-3 minutes.

Mount with glycerin jelly or other aqueous mountant.  Actually, I use a
70% glycerol solution, drop the coverslip on, allow the slide to dry
overnight and apply clear nail-polish around the edges the next day.

Some of this might sound a bit amateurish for those who do full-scale
histology, so please feel free to make suggestions/comments about this
protocol.  The focus of my work is not actually histology, but I like to
implement histological techniques in my characterization of knockout
tissue.  I find it is very informative and oddly enough, highly
under-used by my colleagues who also study targeted gene knockout mice!
How can *anybody* resist pretty pictures!!??? .

Anyway, here is the recipe for the (0.5%) Oil Red O stain:

0.5 g Oil Red O
100 mL propylene glycol

Add small amount of propylene glycol to Oil Red O, with stirring.
Gradually add remaining propylene glycol.  Heat until solution reaches
95 degrees Celsius.  Filter through coarse paper while warm.  Let stand
overnight at room temperature.  Filter again.  Solution can be kept at
RT for years and years and year.

If you need any more info, please feel free to contact me.

Jacqui

Jacqui Detmar, Post-doctoral Fellow
Samuel Lunenfeld Research Institute, room 876
Mount Sinai Hospital
600 University Avenue
Toronto, ON, Canada
M5G 1X5

phone:   416-586-4800 x2451/x2290
fax:        416-586-8588
email:     detmar@mshri.on.ca


From histonet <@t> metasynthesis.net  Thu Mar 27 12:28:37 2008
From: histonet <@t> metasynthesis.net (William Gunn)
Date: Thu Mar 27 12:28:41 2008
Subject: [Histonet] Re: Histonet Digest, Vol 52, Issue 40
In-Reply-To: <20080327170740.A908C20E7E@postalmail-mx3.g.dreamhost.com>
References: <20080327170740.A908C20E7E@postalmail-mx3.g.dreamhost.com>
Message-ID: <73b82faa0803271028p17e2d93k8b4c9abf37a1866a@mail.gmail.com>

>
> We just X-ray the specimens, which avoids the risk of breaking/bending and
> it's easy to do all the samples at once.
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Thu, 27 Mar 2008 07:57:49 -0700 (PDT)
> From: Rene J Buesa 
> Subject: Re: [Histonet] Decal procedures-assessing endpoints
> To: Greg Dobbin , Histonet@lists.utsouthwestern.edu
> Message-ID: <448114.89650.qm@web65708.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> The "softness" of the tissue fragment, how it bends or compresses, will
> give you the most practical way of determining the decal end point.
>  Be careful with the bending to prevent breaking a piece not decalcified
> yet.
>  Ren? J.
>
> Greg Dobbin  wrote:
>  Hello Colleagues,
> Can some of you share with me, your guidelines for determining
> appropriate submersion times for sections of bone or other calcified
> bits in RDO (Trade name) post-fixation but prior to processing
> (obviously). I guess it is a subjective call by necessity!?
> Thanks,
> Greg
>
From koellingr <@t> comcast.net  Thu Mar 27 12:28:49 2008
From: koellingr <@t> comcast.net (koellingr@comcast.net)
Date: Thu Mar 27 12:29:11 2008
Subject: [Histonet] Caspase-3 Antibody; other Abs -- Apoptosis vs. Necrosis
Message-ID: <032720081728.21212.47EBD951000706A4000052DC22007636929D09020704040A0105@comcast.net>

I agree with Patsy about cleaved caspace 3 staining just apoptotic cells.  In a well regulated and defined tissue analysis, TUNEL can certainly be very useful but if you are in an unknown system and tissue milieu where necrosis might, or might not be happening, cleaved caspace-3 is better and discriminates apoptosis from necrosis but there are instances, again in defined systems, where TUNEL is a very, very useful and well established procedure.  The comment about how few cells stain I think is right on.  Programmed cell death is meant absolutely to proceed from death hit to completion very, very quickly.  Cells don't "apoptose" for day's.  So when you fix the cell, you are catching it at one particular moment in time and if not in rapid apoptosis at that moment, you miss it.  In grad school I worked in mouse and primate thymus where 90%?, 95%?, 98%? of thymocytes undergo apoptosis during negative selection.  Did immunoEM and was constantly amazed at how VERY FEW T cells looked so 
good and so VERY FEW were in the classical morphologic throws of apoptosis.  Once they get "the hit" their brief journey to apoptotic debris is amazingly quick.  You are just catching some during fixation that are undergoing morphological apoptosis even is most are destined to do so.

Ray Koelling
PhenoPath Labs
Seattle, WA

 -------------- Original message ----------------------
From: "patsy ruegg" 
> In my experience CC3 does a much better job of staining just apoptotic cells
> and not necrotic cells than Tunnel as a matter of fact I have never doubted
> that the staining I get with CC3 is anything but apoptotic cells which is
> not the case with Tunnel, we could not distinguish necrosis from apoptosis
> with Tunnel.
> 
> Patsy
> 
> One complaint I get from my clients is how few cells stain with CC3, but I
> think it is real and they are used to seeing more cells stain with Tunnel
> because Tunnel is picking up necrotic cells as well as apoptotic cells.
> 
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech
> 12635 Montview Blvd. #216
> Aurora, CO 80010
> 720-859-4060
> fax 720-859-4110
> pruegg@ihctech.net
> www.ihctech.net 
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of GT Hebert
> Sent: Thursday, March 27, 2008 8:03 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Caspase-3 Antibody; other Abs -- Apoptosis vs. Necrosis
> 
> Hello all,
>    
>   Does anyone know if Caspase-3 antibody is suitable for detecting apoptotic
> cells (as opposed to both apoptotic and necrotic cells)?
>    
>   If it cannot distiguish between cells types, is there an antibody (for
> mouse tissue) that can be used or is my only option in situ techniques?
>    
>   Thank you.
>    
>   Gustave Hebert
>   Scientist II
>   Wyeth Research
>   Cambridge MA
> 
>        
> ---------------------------------
> Be a better friend, newshound, and know-it-all with Yahoo! Mobile.  Try it
> now.
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From tjasper <@t> copc.net  Thu Mar 27 12:37:15 2008
From: tjasper <@t> copc.net (Thomas Jasper)
Date: Thu Mar 27 12:37:19 2008
Subject: [Histonet] Floaters, erroneous tissue,
	on a slide or embedded in block
References: <001801c88f4f$2d336370$240c0180@PCLAB11>
Message-ID: <90354A475B420441B2A0396E5008D4965E2093@copc-sbs.COPC.local>

Hey Meredith,

I believe Renee Buesa has commented that "0" is the desired average for
floaters...I agree.  It sounds like you are being diligent about
avoiding them and obviously you're not at zero.  I'd say we are in the
same boat around here.  We are neat and tidy at all workstations and our
pathologists often express their appreciation of this.  We do get that
occasional floater though.  Just wanted to mention...if floaters
originate at the grossing bench, obviously you're not the cause.
Certainly pathologists, PAs, and grossing techs work to avoid them as
well, but it happens.  At my old job, we had a very particular
pathologist that kept a wet sponge on the bench.  He was quite diligent
about keeping the grossing tools clean and clear.  Another trouble spot
may be forceps warmers.  Regularly taking a q-tip or something to them
for cleaning out, helps eliminate a potential floater source.  Also
wiping the forceps off between blocks is helpful.  I miss the old Bunsen
burner days.  I realize, without a doubt, that an open flame in the lab
is, and should be strictly...verboten!  But, you've got to admit it
certainly kept the forceps free of debris.  Also when sectioning, I
stress to our techs, please skim the water bath between blocks.  Even
so, you may get a stray, something or another, catching an edge on a
slide.
Anyway like I said, we're always doing our best to eliminate this
problem, as it seems you are too.  And you may be aware of, and practice
what I've mentioned.  Just wanted you to know you're not alone.  Keep up
the good work.

Have a good day,
Tom J.

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, Oregon 97701
541/693-2677

 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Meredith
Fuller-Fedorczyk
Sent: Wednesday, March 26, 2008 7:39 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Floaters, erroneous tissue,on a slide or embedded in
block

For monitoring risk management we keep a monthly tally of floaters found
on stained slides. Even with cleaning of molds and wiping down embedding
area we still might have a month with two or three incidences. Is there
any data for an average expectation of occurrences per month.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From Shirley_PHUA <@t> hsa.gov.sg  Thu Mar 27 13:04:54 2008
From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA)
Date: Thu Mar 27 13:07:51 2008
Subject: [Histonet] Shirley Phua is out-of-office ...
Message-ID: 

I will be out of the office from  28-03-2008 to 28-03-2008.

I'll be away 28 March 2008 morning.

Pathologists:
I will process your requests when I return. If urgent, please forward your
email to Henry_Kyaw@hsa.gov.sg


From Maxim_71 <@t> mail.ru  Thu Mar 27 13:25:45 2008
From: Maxim_71 <@t> mail.ru (Maxim_71@mail.ru)
Date: Thu Mar 27 13:24:55 2008
Subject: [Histonet] Decal procedures-assessing endpoints
Message-ID: <179511867.20080327212545@mail.ru>

Greg,
The best method for endpoint of decal in my opinion
is "Weight loss, weight gain", if you can not have
Faxitron and etc. It is require some
simple instruments: the scales, pencil, filter
paper... and few seconds for one object.
It is suitable for any type decal fluid, include EDTA.

Maxim Peshkov,
Russia,
Taganrog.

mailto:Maxim_71@mail.ru


From schaundrawalton <@t> yahoo.com  Thu Mar 27 13:39:42 2008
From: schaundrawalton <@t> yahoo.com (Schaundra Walton)
Date: Thu Mar 27 13:39:50 2008
Subject: [Histonet] Validation Studies
Message-ID: <735522.40440.qm@web58912.mail.re1.yahoo.com>

Dear Histonetters,
   
  We are looking at bringing in a Ventana Benchmark XT IHC stainer.  We currently use the Ventana Nexus IHC stainer.  Has anyone else made this transition or simply added a new IHC stainer lately?  How did you validate the new equipment?  Is there a certain number of slides or cases you had to run?  Any help would be much appreciated.
   
  Thanks,
   


Schaundra Walton BS HTL(ASCP)
Swedish American Hospital
1401 E. State St. 
Rockford, IL 61104
       
---------------------------------
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From elizabeth.heimrich <@t> bms.com  Thu Mar 27 13:42:14 2008
From: elizabeth.heimrich <@t> bms.com (Elizabeth M Heimrich)
Date: Thu Mar 27 13:42:21 2008
Subject: [Histonet] Excelsior processor in Biotech/Pharma facilities
In-Reply-To: 
References: 
Message-ID: <47EBEA86.7050401@bms.com>

Hi Tracy,
We have both VIP and Excelsior...I like them both, the Excelsior is 
easier to change reagents. Just attach a reagent bottle, instead of 
pouring the reagents into containers, the baskets are a little different 
though, they take a littlle to get used to.  I've processed all my 
tissues on both machines and I have not noticed a difference.  Rat paws 
ti small Lymph nodes....Demo the excelsior before you buy...You are all 
familar w/ VIP, so test drive the Excelsior!
Beth

Tracy Bergeron wrote:

>Hi folks,
>
>        We are looking into getting a new processor in our lab.  We are 
>trying to decide between the VIP and the Excelsior.  Several of us are 
>most familiar with the VIP, and like the ease of use and # of cassettes it 
>holds, the rest of our group though familiar with the VIP like the idea of 
>conserving reagents by using the Excelsior. 
>
>That said
>I am looking for information from the biotech/Pharma community on the 
>Excelsior. 
>Is there anyone out there using this processor outside the clinical lab?
>Does it work well on rodent tissue?
>What about hard tissues?
>Bone
>Paws (mouse and rat)
>
>My next question is for people familiar with the earlier models of the 
>Excelsior as well as the newest model. (clinical or research)
>I find the software for the older model very cumbersome and not user 
>friendly.
>Is the software on the newer machines any easier to use for the average 
>person?
>Is it as easy as using the VIP, or Pathcenter? - follow the prompts sort 
>of thing.
> 
>I would appreciate any input folks have.
>Thanks.
>
>Sincerely,
>Tracy E. Bergeron, B.S., HT, HTL (ASCP)
>Associate Scientist III, Pathology
>Comparative Pathology Laboratory
>Biogen Idec
>14 Cambridge Center
>Cambridge, MA 02142
>Direct:  617-914-1115
>Fax:  617-679-3208
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>  
>
From hej01 <@t> health.state.ny.us  Thu Mar 27 14:01:26 2008
From: hej01 <@t> health.state.ny.us (Helen E Johnson)
Date: Thu Mar 27 14:01:33 2008
Subject: [Histonet] quenching GFP fluorescence
Message-ID: 


Hi Histonetters,
      How would you quench GFP fluorescence prior to IHC?
                                    Helen Johnson
(hej01@health.state.ny.us)


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From gayle.callis <@t> bresnan.net  Thu Mar 27 14:03:45 2008
From: gayle.callis <@t> bresnan.net (Gayle Callis)
Date: Thu Mar 27 14:03:48 2008
Subject: [Histonet] non messy Oil Red O staining
Message-ID: <000801c8903d$48520a00$6401a8c0@DHXTS541>

This method developed by Chuck Churukian is less messy than the oil red O stain using propylene glycol.   We have used it for fresh tissue frozen sections, fixed in NBF OR in formalin fixed tissue, snap frozen, cut and then stained.  Chuck published this in J of Histotechnology and it also is found in Gamble and Bancrofts 5th and 6th editions of Theory and Practice of Histological Technique.  You microbiology department may have the correct dextrin available.  

Oil Red O/Dextrin, Churukian method

 

Frozen sections fixed post cutting with NBF can be rinsed and stained immediately, or NBF fixed tissue frozen sections (cryoprotected), mounted on Plus Charge slides. Air dry NBF fixed frozen sections 30 min to 1 hour, or longer to insure they stay on slide.  Fresh tissue frozen sections can be fixed 10 minutes or so before staining, but treat these gently. 

Protocol:

1.         Immerse dry slides directly into filtered 0.5% Oil Red O in Dextrin , stain 20 minutes

2.         Rinse VERY GENTLY in running tap water

3.         Counterstain with Gill II hematoxylin for 20 - 30 seconds

4.         Rinse gently with water, blue in bluing solution, (NOT AMMONIA WATER), rinse gently, and coverslip with aqueous mounting media



Reagents:

Dissolve 0.5 gm Oil Red O in absolute isopropyl alcohol, allow to stir overnight.



Dissolve 1 gm dextrin (bacteriological grade or TYPE III (Sigma) from corn in 100 ml distilled water



Working solution is 60 mls stock Oil Red O and 40 ml 1% dextrin solution



Stable for months, and reported to work on paraffin sections.



Reference:    C. Churukian, Lillie's Oil Red O for neutral lipids, J Histotechnology 22(4):309, 1999.



Gayle M. Callis

HTL/HT/MT(ASCP)

Bozeman MT 59715
From froyer <@t> bitstream.net  Thu Mar 27 14:11:04 2008
From: froyer <@t> bitstream.net (Ford Royer)
Date: Thu Mar 27 14:11:11 2008
Subject: [Histonet] Excelsior processor in Biotech/Pharma facilities
In-Reply-To: <47EBEA86.7050401@bms.com>
References: 
	<47EBEA86.7050401@bms.com>
Message-ID: <000601c8903e$4e0c7330$7701a80a@Ford>

If I am not having a "Senior Moment", on the VIP-5*; I believe that you can
fill and empty the reagent containers "on the instrument" using an ancillary
hose and a drain port.  You do not have to pour reagents into the containers
*(VIP-5 only... not the older VIP-"E" Series or the "K" series)

Ford M. Royer, MT(ASCP)
Minnesota Medical, Inc.
7177 Madison Ave. W.
Golden Valley, MN 55427-3601

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth M
Heimrich
Sent: Thursday, March 27, 2008 1:42 PM
To: Tracy Bergeron
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Excelsior processor in Biotech/Pharma facilities

Hi Tracy,
We have both VIP and Excelsior...I like them both, the Excelsior is 
easier to change reagents. Just attach a reagent bottle, instead of 
pouring the reagents into containers, the baskets are a little different 
though, they take a littlle to get used to.  I've processed all my 
tissues on both machines and I have not noticed a difference.  Rat paws 
ti small Lymph nodes....Demo the excelsior before you buy...You are all 
familar w/ VIP, so test drive the Excelsior!
Beth

Tracy Bergeron wrote:

>Hi folks,
>
>        We are looking into getting a new processor in our lab.  We are 
>trying to decide between the VIP and the Excelsior.  Several of us are 
>most familiar with the VIP, and like the ease of use and # of cassettes it 
>holds, the rest of our group though familiar with the VIP like the idea of 
>conserving reagents by using the Excelsior. 
>
>That said
>I am looking for information from the biotech/Pharma community on the 
>Excelsior. 
>Is there anyone out there using this processor outside the clinical lab?
>Does it work well on rodent tissue?
>What about hard tissues?
>Bone
>Paws (mouse and rat)
>
>My next question is for people familiar with the earlier models of the 
>Excelsior as well as the newest model. (clinical or research)
>I find the software for the older model very cumbersome and not user 
>friendly.
>Is the software on the newer machines any easier to use for the average 
>person?
>Is it as easy as using the VIP, or Pathcenter? - follow the prompts sort 
>of thing.
> 
>I would appreciate any input folks have.
>Thanks.
>
>Sincerely,
>Tracy E. Bergeron, B.S., HT, HTL (ASCP)
>Associate Scientist III, Pathology
>Comparative Pathology Laboratory
>Biogen Idec
>14 Cambridge Center
>Cambridge, MA 02142
>Direct:  617-914-1115
>Fax:  617-679-3208
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>  
>


From liz <@t> premierlab.com  Thu Mar 27 14:19:56 2008
From: liz <@t> premierlab.com (Liz Chlipala)
Date: Thu Mar 27 14:20:11 2008
Subject: [Histonet] Validation Studies
In-Reply-To: <851ACC090DB44BB5A6E2D5A4F4162E15@PremierLab.local>
References: <851ACC090DB44BB5A6E2D5A4F4162E15@PremierLab.local>
Message-ID: 

Schanudra

What we do on all of our major equipment for equipment validation such
as the tissue processor, slide stainer and our dako autostainer is we
perform an IOQ or an Installation/Operational Qualification Protocol. We
do this primarily to be able to run GLP studies on this equipment. Its
very detailed and goes over everything that a particular piece of
equipment should do and then verifiying that is does what it says it
will do.   The table of contents is listed below for the dako
autostainer:  As you can see it is quite detailed.  I'm not sure what is
required for JCAHO standards, but this is what we do for GLP compliance.

1.0	General Objective(s)	4
2.0	Scope	4
3.0	Acceptance Criteria and Expected Outcomes	4
4.0	System Description and General Information	4
5.0	IOQ Protocol Execution Procedures and Test Failure Handling
7
6.0	Related Documents	8
7.0	DAKO Autostainer Universal Staining System Installation /
Operational Qualification Test Cases	9
8.0	IOQ-1 Pre-requisite Documents Verification	10
9.0	IOQ-2 Operation and Maintenance Manuals Verification	13
10.0	IOQ-3 System Environment Verification	17
11.0	IOQ-4 Utilities Verification	22
12.0	IOQ-5 Major Components Verification	25
13.0	IOQ-6 Hardware Verification	30
14.0	IOQ-7 Software Verification	33
15.0	IOQ-8 System Operations and Functions Verification	36
16.0	Completion of Installation/Operational Qualification Tests
44
17.0	Signature Table	45
18.0	Attachments	46

Liz
 

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
liz@premierlab.com
www.premierlab.com
 
Ship to Address:
 
Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Schaundra Walton
Sent: Thursday, March 27, 2008 12:46 PM
To: Histonet
Subject: [Histonet] Validation Studies

Dear Histonetters,
   
  We are looking at bringing in a Ventana Benchmark XT IHC stainer.  We
currently use the Ventana Nexus IHC stainer.  Has anyone else made this
transition or simply added a new IHC stainer lately?  How did you
validate the new equipment?  Is there a certain number of slides or
cases you had to run?  Any help would be much appreciated.
   
  Thanks,
   


Schaundra Walton BS HTL(ASCP)
Swedish American Hospital
1401 E. State St. 
Rockford, IL 61104
       
---------------------------------
Looking for last minute shopping deals?  Find them fast with Yahoo!
Search.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




From gayle.callis <@t> bresnan.net  Thu Mar 27 14:42:29 2008
From: gayle.callis <@t> bresnan.net (Gayle Callis)
Date: Thu Mar 27 14:42:34 2008
Subject: [Histonet] Submersion time in acid decalcifier - long reply
Message-ID: <001201c89042$b1391a90$6401a8c0@DHXTS541>

Greg,  

The only way to guarantee a bone is not overexposed to acid is to perform an endpoint test.  RDO has approximately 15% hydrochloric acid plus additives, so it is very fast, plus the color is very dark making it difficult to see the bone in the decalcifier.  However, it can be diluted with water,  even in half and still remain a rapid decalcifier with excellent staining results, but the endpoint test is needed.  

Subjective testing is not considered accurate and these tests include mechanical testing where probing can create a probe track artifact in the bone, bending can disrupt tumor from bone or interface of bone and tissues/cells, plus create false trabecular fracture artifact.  Slicing the bone to feel gritty-ness, this is accurate for detecting very tiny calcifications, or you can just plain miss them, same with a probe.  

Bubble testing is inaccurate - as the calcium ionizes, CO2 is given off too, and resides as bubbles on the surface of the bone.  As the calcium deposits become smaller the bubbles are smaller, and hard to see.  One lab where I worked  used this for tiny bone marrow biopsies which would float upwards at end of decalcification, however, there was often residual calcium deposits in bone, requiring surface decalcification of block face.   Seeing bubbles in RDO is going to be difficult due to the color.   

The most sensitive method is radiography with a FAXITRON, but most labs do not have this enclosed x-ray machine.  We, unfortunately, gave our FAXITRON away, a terrible mistake and had to rely on another test which works well, although not the most accurate way to test. 

Either do the chemical test which is easy to do, just read the test fluid against a black background, an old med tech trick, to detect cloudy ppt and use a clear water blank for comparison.  Do not stir the fluid when doing this test and only one bone per container for accuracy,  The aliquot should come from container bottom where Calcium settles during decalcification.  If the bone is not decalcified, then you should rinse with tap water briefly, as calcium ionized by acid can reside on bone surfaces.  You don't want that to happen when you put bone back into fresh decalcifier.  

We now use the weight loss/weight gain method (developed for testing nitric acid, and was coupled with chemical testing at the very end.)   We do not bother with further chemical test after the weight it gained.    It works well, since one can stir (this also causes bubbles to disperse) and do more than one bone, suspended in decalcifying fluid, in a container.  Often we have 50 murine bones in one container.  The balance must weigh in milligrams for accuracy, and you need to blot bone with paper towel before weighing.  The joy of this method is speed and efficiency and works perfectly well for EDTA decalcification which we do frequently for research bone protocols.    

If you want the latter two methods, I will be happy to send them privately. 

Whatever you do, don't "overdecalcify" the bones. 

Gayle M. Callis
HTL/HT/MT(ASCP)
Bozeman MT 59715
 

From cinrica <@t> embarqmail.com  Thu Mar 27 14:43:47 2008
From: cinrica <@t> embarqmail.com (Glidden M marlene rivera)
Date: Thu Mar 27 14:43:54 2008
Subject: [Histonet] looking for temp traveling mohs tech jobs
Message-ID: <33242142.1321821206647027861.JavaMail.root@md34.embarq.synacor.com>



Hi everyone can someone give me some names of good companys that are paying mohs techs. to travel for temp jobs? 

Thanks in advance for your help...Marlene 
From gayle.callis <@t> bresnan.net  Thu Mar 27 14:49:48 2008
From: gayle.callis <@t> bresnan.net (Gayle Callis)
Date: Thu Mar 27 14:49:53 2008
Subject: Why do you need to Re: [Histonet] quenching GFP fluorescence
References: 
Message-ID: <001801c89043$b6e049e0$6401a8c0@DHXTS541>

Why do you need to get rid of GFP fluorescence if you are going to do IHC? 
IHC is based on using chromogen with enzyme immunohistochemistry and if you 
are not looking at your IHC protocol with a fluorescent microscope, but 
rather with  light microscopy, then GFP will not be seen - that would be 
more of a problem with doing immunofluorescent staining (IFA).

If you mean doing another immunofluorscent stain for different tissue 
component, or cell, then use a different color fluorophore i.e. red  than 
GFP, then you should not have GFP showing up, unless you switch to a GFP 
filter (you could have double fluorescence then).

Maybe I am not understanding what you want to do here.

Gayle M. Callis
HTL/HT/MT(ASCP)
Bozeman MT 597175


----- Original Message ----- 
From: "Helen E Johnson" 
To: 
Sent: Thursday, March 27, 2008 1:01 PM
Subject: [Histonet] quenching GFP fluorescence


>
> Hi Histonetters,
>      How would you quench GFP fluorescence prior to IHC?
>                                    Helen Johnson
> (hej01@health.state.ny.us)
>
>
> IMPORTANT NOTICE:  This e-mail and any attachments may contain 
> confidential or sensitive information which is, or may be, legally 
> privileged or otherwise protected by law from further disclosure.  It is 
> intended only for the addressee.  If you received this in error or from 
> someone who was not authorized to send it to you, please do not 
> distribute, copy or use it or any attachments.  Please notify the sender 
> immediately by reply e-mail and delete this from your system. Thank you 
> for your cooperation.
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


From cinrica <@t> embarqmail.com  Thu Mar 27 16:24:34 2008
From: cinrica <@t> embarqmail.com (Glidden M marlene rivera)
Date: Thu Mar 27 16:24:39 2008
Subject: [Histonet] (no subject)
Message-ID: <1125495572.1345931206653074823.JavaMail.root@md34.embarq.synacor.com>



Thank you Jose O. for your responding to my needing info.? on how to find some companies who will hire mohs techs to travel. 

However I went to the web site you gave and no success....let me know how the first trip goes for you...good luck!Thanks Marlene
From williamstewart.pathology <@t> gmail.com  Thu Mar 27 16:28:36 2008
From: williamstewart.pathology <@t> gmail.com (William Stewart)
Date: Thu Mar 27 16:28:40 2008
Subject: [Histonet] Hematoxylin Shortage??
Message-ID: <81a8916f0803271428m130df056s1e775ba17025736b@mail.gmail.com>

Histoneters:
I heard a rumor through a vendor that there may be a hematoxylin shortage.
Has anyone heard this or experienced a back order from a vendor?

Thanks you!
Bill


*William R. Stewart
*Department of Pathology
Manager, Anatomic Pathology
University of Pittsburgh Medical Centers
Shadyside Hospital
Tel: 412-623-3281
Pager: 412-958-4171
*stewartwr@upmc.edu
<*mailto:stewartwr@upmc.edu
*>*
From CrochiereSteve <@t> aol.com  Thu Mar 27 19:59:58 2008
From: CrochiereSteve <@t> aol.com (CrochiereSteve@aol.com)
Date: Thu Mar 27 20:00:15 2008
Subject: [Histonet] workload units
Message-ID: 

I assigned my own "units" basing it roughly on the amount of hands on tech 
time per task in minutes.
It may not be the "official," but it gives me an idea of who does more or 
less work and who can handle the workload in my off site lab. It also spark a 
little competition among the techs which does get ugly on occasion.
 
Steven M. Crochiere, HT(ASCP)
Histology Supervisor
LifePath Partners @ Mercy Medical Center
Springfield, MA 01104




**************Create a Home Theater Like the Pros. Watch the video on AOL 
Home.      
(http://home.aol.com/diy/home-improvement-eric-stromer?video=15&ncid=aolhom00030000000001)
From kadamsplw <@t> gmail.com  Fri Mar 28 02:47:30 2008
From: kadamsplw <@t> gmail.com (karen adams)
Date: Fri Mar 28 02:47:35 2008
Subject: [Histonet] Happy Friday :)
Message-ID: 

...I am going to sip my coffee and plan my strategy for the hematoxylin
shortage and the truckers strike....Happy Friday All !!

-- 
Karen Adams
Pathology Laboratories West
9303 Park West Blvd.
Knoxville, TN 37923
From rjr6 <@t> psu.edu  Fri Mar 28 07:21:41 2008
From: rjr6 <@t> psu.edu (Roberta Horner)
Date: Fri Mar 28 07:21:47 2008
Subject: [Histonet] Hematoxylin Shortage??
In-Reply-To: <81a8916f0803271428m130df056s1e775ba17025736b@mail.gmail.com>
References: <81a8916f0803271428m130df056s1e775ba17025736b@mail.gmail.com>
Message-ID: 

I have also heard this from a vendor.
Roberta

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of William
Stewart
Sent: Thursday, March 27, 2008 5:29 PM
To: Histonet
Subject: [Histonet] Hematoxylin Shortage??

Histoneters:
I heard a rumor through a vendor that there may be a hematoxylin
shortage.
Has anyone heard this or experienced a back order from a vendor?

Thanks you!
Bill


*William R. Stewart
*Department of Pathology
Manager, Anatomic Pathology
University of Pittsburgh Medical Centers
Shadyside Hospital
Tel: 412-623-3281
Pager: 412-958-4171
*stewartwr@upmc.edu
<*mailto:stewartwr@upmc.edu
*>*
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From talulahgosh <@t> gmail.com  Fri Mar 28 08:25:01 2008
From: talulahgosh <@t> gmail.com (Emily Sours)
Date: Fri Mar 28 08:26:28 2008
Subject: Fwd: [Histonet] Hematoxylin Shortage??
In-Reply-To: 
References: <81a8916f0803271428m130df056s1e775ba17025736b@mail.gmail.com>
	
	
Message-ID: 

Why would this happen?
Is this like that one goat dying at Santa Cruz?

Emily
-- 
People aren't like chocolates. People are bastards. Bastards with
bastard coating and bastard filling.

From Susan.Weber2 <@t> va.gov  Fri Mar 28 08:34:44 2008
From: Susan.Weber2 <@t> va.gov (Weber, Susan (VHACLE))
Date: Fri Mar 28 08:34:49 2008
Subject: [Histonet] Excelsior processor in Biotech/Pharma facilities
In-Reply-To: 
References: 
	
Message-ID: <16C83872A53F4346AA9C3A18E3A3AAB903F76D55@VHAV10MSGA1.v10.med.va.gov>

Hello Tracy!
Although I am not a current user of the Excelsior, I used the earlier
generation machine for quite some time and was responsible for the
purchase of it. I found that for a low volume lab/small staff lab, the
Excelsior was a wonderful product especially when utilizing the rotation
system properly. When you get past the concept of seeing the reagent
bottles and rely on the processor to "do it's own thing" ie; rotation by
alcohol quality etc. It is a great machine. You must ALWAYS! ALWAYS!
remove the used, old , crummy reagents when it rotates...if not it is a
Disaster!!! I felt it was a reliable machine and my co-worker and I
really liked it (I do not know the difference between the "old vs new
software". I also liked the NetMon feature of the equipment and was a
beta testing site for home monitoring. (My PC died and they found out
that the software they were using was not Mac friendly! Most software,
games, programs, etc. aren't PC and Mac anyway!) In a high
volume/multi-user environment, it seemed that some techs had a hard time
with the rotation management of the Excelsior. I also use the VIP and as
usual, it is a reliable workhorse. I really felt that the Excelsior
saved money, and reagent use and most definitely tech time. 

Susan M Weber HT(ASCP)
Histology Supervisor
Louis Stokes Cleveland VA Medical Center
10701 East Blvd
Cleveland, Ohio 44106
(216) 791-3800 X6154

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tracy
Bergeron
Sent: Thursday, March 27, 2008 11:26 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Excelsior processor in Biotech/Pharma facilities

Hi folks,

        We are looking into getting a new processor in our lab.  We are 
trying to decide between the VIP and the Excelsior.  Several of us are 
most familiar with the VIP, and like the ease of use and # of cassettes
it 
holds, the rest of our group though familiar with the VIP like the idea
of 
conserving reagents by using the Excelsior. 

That said
I am looking for information from the biotech/Pharma community on the 
Excelsior. 
Is there anyone out there using this processor outside the clinical lab?
Does it work well on rodent tissue?
What about hard tissues?
Bone
Paws (mouse and rat)

My next question is for people familiar with the earlier models of the 
Excelsior as well as the newest model. (clinical or research)
I find the software for the older model very cumbersome and not user 
friendly.
Is the software on the newer machines any easier to use for the average 
person?
Is it as easy as using the VIP, or Pathcenter? - follow the prompts sort

of thing.
 
I would appreciate any input folks have.
Thanks.

Sincerely,
Tracy E. Bergeron, B.S., HT, HTL (ASCP)
Associate Scientist III, Pathology
Comparative Pathology Laboratory
Biogen Idec
14 Cambridge Center
Cambridge, MA 02142
Direct:  617-914-1115
Fax:  617-679-3208
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From marktarango <@t> gmail.com  Fri Mar 28 09:11:51 2008
From: marktarango <@t> gmail.com (Mark Tarango)
Date: Fri Mar 28 09:11:56 2008
Subject: [Histonet] Hematoxylin Shortage??
In-Reply-To: 
References: <81a8916f0803271428m130df056s1e775ba17025736b@mail.gmail.com>
	
	
	
Message-ID: <5b6eb13e0803280711k505515e7g43045e69f022ab91@mail.gmail.com>

I don't know.  This could be a clever trick from industry to drive the
hematoxylin price sky-high!  No hematoxylin on Wednesdays, everyone switch
to celestine blue!

or... maybe global warming is killing off the trees... (wait, do we still
get it from trees?)

Who knows, but the sales guy has to have something to say when he drops in,
right?  If there isn't a new merger going on this week, why not a
hematoxylin shortage?

On Fri, Mar 28, 2008 at 6:25 AM, Emily Sours  wrote:

> Why would this happen?
> Is this like that one goat dying at Santa Cruz?
>
> Emily
> --
> People aren't like chocolates. People are bastards. Bastards with
> bastard coating and bastard filling.
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
From lblazek <@t> digestivespecialists.com  Fri Mar 28 09:32:03 2008
From: lblazek <@t> digestivespecialists.com (Blazek, Linda)
Date: Fri Mar 28 09:28:20 2008
Subject: [Histonet] Hematoxylin Shortage??
In-Reply-To: <5b6eb13e0803280711k505515e7g43045e69f022ab91@mail.gmail.com>
References: <81a8916f0803271428m130df056s1e775ba17025736b@mail.gmail.com>
	<5b6eb13e0803280711k505515e7g43045e69f022ab91@mail.gmail.com>
Message-ID: <1F937FB30BDB7C4A9F39F83FEA8D379F9F537E@bruexchange1.digestivespecialists.com>

I'd be interested in knowing which sales reps (companies) are saying
there is going to be a shortage.
Linda

Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 293-4424 ext 7118
Email: lblazek@digestivespecialists.com

 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark
Tarango
Sent: Friday, March 28, 2008 10:12 AM
To: Emily Sours
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Hematoxylin Shortage??

I don't know.  This could be a clever trick from industry to drive the
hematoxylin price sky-high!  No hematoxylin on Wednesdays, everyone
switch
to celestine blue!

or... maybe global warming is killing off the trees... (wait, do we
still
get it from trees?)

Who knows, but the sales guy has to have something to say when he drops
in,
right?  If there isn't a new merger going on this week, why not a
hematoxylin shortage?

On Fri, Mar 28, 2008 at 6:25 AM, Emily Sours 
wrote:

> Why would this happen?
> Is this like that one goat dying at Santa Cruz?
>
> Emily
> --
> People aren't like chocolates. People are bastards. Bastards with
> bastard coating and bastard filling.
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From Barry.R.Rittman <@t> uth.tmc.edu  Fri Mar 28 09:47:13 2008
From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R)
Date: Fri Mar 28 09:47:19 2008
Subject: [Histonet] Hematoxylin Shortage??
In-Reply-To: <1F937FB30BDB7C4A9F39F83FEA8D379F9F537E@bruexchange1.digestivespecialists.com>
References: <81a8916f0803271428m130df056s1e775ba17025736b@mail.gmail.com>
	
	
	
	<5b6eb13e0803280711k505515e7g43045e69f022ab91@mail.gmail.com>
	<1F937FB30BDB7C4A9F39F83FEA8D379F9F537E@bruexchange1.digestivespecialists.com>
Message-ID: 

I am surprised that with the cost of hematoxylin powder as high as it is
that the USA is not considering growing its own supply of Hematoxylon
campechianum (we do have some areas that would be a suitable climate for
cultivation of the trees). We could also outsource this to Mexico as I
believe that these trees still grow there in the state of Campeche.
After all the Aztecs prepared their own hematoxylin for their purple
robes for quite some time. (I am not suggesting that we use the old
methods of preparation such as soaking the heartwood of the tree in
urine to help the weathering).
Barry

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek,
Linda
Sent: Friday, March 28, 2008 9:32 AM
To: Mark Tarango; Emily Sours
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Hematoxylin Shortage??

I'd be interested in knowing which sales reps (companies) are saying
there is going to be a shortage.
Linda

Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 293-4424 ext 7118
Email: lblazek@digestivespecialists.com

 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark
Tarango
Sent: Friday, March 28, 2008 10:12 AM
To: Emily Sours
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Hematoxylin Shortage??

I don't know.  This could be a clever trick from industry to drive the
hematoxylin price sky-high!  No hematoxylin on Wednesdays, everyone
switch
to celestine blue!

or... maybe global warming is killing off the trees... (wait, do we
still
get it from trees?)

Who knows, but the sales guy has to have something to say when he drops
in,
right?  If there isn't a new merger going on this week, why not a
hematoxylin shortage?

On Fri, Mar 28, 2008 at 6:25 AM, Emily Sours 
wrote:

> Why would this happen?
> Is this like that one goat dying at Santa Cruz?
>
> Emily
> --
> People aren't like chocolates. People are bastards. Bastards with
> bastard coating and bastard filling.
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From integrated.histo <@t> gmail.com  Fri Mar 28 10:52:27 2008
From: integrated.histo <@t> gmail.com (Cindy DuBois)
Date: Fri Mar 28 10:52:37 2008
Subject: [Histonet] Liquid waste disposal, CA
Message-ID: <5d9104a30803280852p2e68d864x15de7fed3e5aa296@mail.gmail.com>

If you are in California, what liquid waste hauling company are you using
for your hazardous waste disposal?

Cindy Dubois
Integrated Pathology
Stockton, CA
From integrated.histo <@t> gmail.com  Fri Mar 28 11:12:54 2008
From: integrated.histo <@t> gmail.com (Cindy DuBois)
Date: Fri Mar 28 11:12:59 2008
Subject: [Histonet] Tissue processors
Message-ID: <5d9104a30803280912u61eb0accm732da2fe1433163e@mail.gmail.com>

I know this subject has been passed around many times, but I can't find
anything recent in the archives.
My boss wants me to reasearch rapid processors vs. standard processors.  His
specific question was is it more practical to buy one rapid processor or two
standard processors.  We have 2 VIP's now (2000 & 3000) that are about 15 &
20 years old. We currently run both processors with w/ different
programs  to accomodate fatty, thick specimens vs. biopsies.
We will need to replace them soon.
Are the VIP's still good processors, or are there others I should be looking
at?

I will also welcome information from sales reps.

Cindy DuBois
Integrated Pathology
Stockton, CA
From making <@t> ufl.edu  Fri Mar 28 12:15:33 2008
From: making <@t> ufl.edu (MKing)
Date: Fri Mar 28 12:17:01 2008
Subject: [Histonet] quenching GFP fluorescence
Message-ID: <47ED27B5.20700@ufl.edu>

Helen,
If you're using DAB as a peroxidase substrate for your IHC the GFP 
fluorescence will be eliminated.
Mike King
----------
Message: 9
Date: Thu, 27 Mar 2008 15:01:26 -0400
From: Helen E Johnson 
Subject: [Histonet] quenching GFP fluorescence
To: histonet@lists.utsouthwestern.edu
Message-ID:
	
	
Content-Type: text/plain; charset=US-ASCII


Hi Histonetters,
       How would you quench GFP fluorescence prior to IHC?
                                     Helen Johnson
(hej01@health.state.ny.us)


From Jason.Burrill <@t> crl.com  Fri Mar 28 12:22:15 2008
From: Jason.Burrill <@t> crl.com (Burrill, Jason)
Date: Fri Mar 28 12:22:25 2008
Subject: [Histonet] RE: Liquid waste disposal, CA
In-Reply-To: 
References: 
Message-ID: <1AD4E907E9B6F648AEF1B3A20A9B0E1EDF5DD2@shr-exch2.na01.crl.com>

Cindy,

I don't work in CA but we do have facilities in CA and the company that
we contract out our hazardous waste to is Veolia Environmental Services.
They are a worldwide company that has a very good reputation.

 

Here is a link to their website http://veoliaes-ts.com/Home and if you
are interested in speaking with someone at Veolia you can contact Greg
Giandomenico at: 

 

Veolia ES Technical Solutions, L.L.C.

9530 Candida Street

San Diego, CA 92126

Office: (800) 956-5782

Cell Phone: (508) 878-4802

Fax: (858) 244-0492 Veolia 

Greg.Giandomenico@veoliaes.com 

 

Jason Burrill

Sr. Manager, Histology and Laboratory Safety

Charles River Laboratories

251 Ballardvale Street

Wilmington, MA 01887

Ph: 781-222-6152

Fax: 978-988-8793

jason.burrill@crl.com 

**Please note new direct dial telephone number**

 

 

 

----------------------------------------------------------------------

 

Message: 1

Date: Fri, 28 Mar 2008 08:52:27 -0700

From: "Cindy DuBois" 

Subject: [Histonet] Liquid waste disposal, CA

To: histonet@lists.utsouthwestern.edu

Message-ID:

 
<5d9104a30803280852p2e68d864x15de7fed3e5aa296@mail.gmail.com>

Content-Type: text/plain; charset=ISO-8859-1

 

If you are in California, what liquid waste hauling company are you
using

for your hazardous waste disposal?

 

Cindy Dubois

Integrated Pathology

Stockton, CA

 

 

From JMacDonald <@t> mtsac.edu  Fri Mar 28 12:58:00 2008
From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald)
Date: Fri Mar 28 12:58:13 2008
Subject: [Histonet] quenching GFP fluorescence
In-Reply-To: <47ED27B5.20700@ufl.edu>
Message-ID: 

I'm not sure that this matters for this, but DAB is not a substrate, but a 
chromogen.  The actual substrate is hydrogen peroxide.




MKing  
Sent by: histonet-bounces@lists.utsouthwestern.edu
03/28/2008 10:21 AM

To
histonet@lists.utsouthwestern.edu
cc

Subject
[Histonet] quenching GFP fluorescence






Helen,
If you're using DAB as a peroxidase substrate for your IHC the GFP 
fluorescence will be eliminated.
Mike King
----------
Message: 9
Date: Thu, 27 Mar 2008 15:01:26 -0400
From: Helen E Johnson 
Subject: [Histonet] quenching GFP fluorescence
To: histonet@lists.utsouthwestern.edu
Message-ID:
 

 
Content-Type: text/plain; charset=US-ASCII


Hi Histonetters,
       How would you quench GFP fluorescence prior to IHC?
                                     Helen Johnson
(hej01@health.state.ny.us)


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From linhines <@t> yahoo.com  Fri Mar 28 14:37:20 2008
From: linhines <@t> yahoo.com (Linda Hines)
Date: Fri Mar 28 14:37:25 2008
Subject: [Histonet] AFB Stainng Problem
Message-ID: <195155.39173.qm@web55414.mail.re4.yahoo.com>

Hello, Everyone
   The AFB staining upon competion has little red and blue dots on the control and the patient tissue slides. We filter both the carbol-fuchsin and the methylene blue. Slides are deparafinized and brought to distilled water. The carbol-fuchsin and the methylene blue are from Sigma-Aldrich.Has anyone else had this pricipitate problem? How was it solved? 
  Thanx for the input.
  Linda Hines HT

       
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From rjbuesa <@t> yahoo.com  Fri Mar 28 14:46:58 2008
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Fri Mar 28 14:47:05 2008
Subject: [Histonet] Tissue processors
In-Reply-To: <5d9104a30803280912u61eb0accm732da2fe1433163e@mail.gmail.com>
Message-ID: <121674.98498.qm@web65707.mail.ac4.yahoo.com>

Cindy:
Under separate cover I am sending an article I published in Annals of Diagnostic Pathology on this subject.
  Ren? J.

Cindy DuBois  wrote:
  
 



       
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Never miss a thing.   Make Yahoo your homepage.
From hoaglin5037 <@t> comcast.net  Fri Mar 28 15:05:35 2008
From: hoaglin5037 <@t> comcast.net (hoaglin5037@comcast.net)
Date: Fri Mar 28 15:05:40 2008
Subject: [Histonet] Re: Liquid waste disposal, CA (Cindy DuBois)
Message-ID: <032820082005.3941.47ED4F8F00044B4200000F652212020784C9CCCFCA020704090E0108@comcast.net>

Cindy,  Try Safety Kleen  http://www.safety-kleen.com/Pages/SKHome.aspx they are probably your best bet.

Eric P. Hoaglin
Safety Officer
Unipath LLC
From hoaglin5037 <@t> comcast.net  Fri Mar 28 15:07:21 2008
From: hoaglin5037 <@t> comcast.net (hoaglin5037@comcast.net)
Date: Fri Mar 28 15:07:25 2008
Subject: [Histonet] Re:Tissue processors
Message-ID: <032820082007.9242.47ED4FF90003D7E90000241A2212020784C9CCCFCA020704090E0108@comcast.net>

Cindy,

As a user of both the VIP 5 and VIPe, if you were to switch and just stay with the standard processor the VIP5 is the way to go. Very solid workhorse, we have five machines, with very little problems. 

As far as the rapid processors go Sakura now offers 2 versions a larger one that processes somewhere in the neighborhood of 120 cassettes an hour. The other one, released in Febuary, is small and only uses a powdered reagent and a parafin. Our lab is looking into purchasing the smaller version, due to the lack of histotechs in our area.

Good Luck,

Eric
From BSylinda <@t> aol.com  Fri Mar 28 15:26:12 2008
From: BSylinda <@t> aol.com (BSylinda@aol.com)
Date: Fri Mar 28 15:26:24 2008
Subject: [Histonet] Immuno on decaled toenail????
Message-ID: 

Hello Histoland,
Is there any techs that have performed an immuno stains such as S100 on a  
toenail?  Had any problems with staining after using a decal  solution?  I have 
stained twice and pathologist is not happy with staining  pattern.  This is 
the first nail that I've had to do a immunohistochemical  stain on to rule out 
melanoma.  Any other advice on softening  toenail?  Sorry for all of the 
questions, but I know this is a great place  to start.  Thanks in advance.
 
Sylinda Battle
 



**************Create a Home Theater Like the Pros. Watch the video on AOL 
Home.      
(http://home.aol.com/diy/home-improvement-eric-stromer?video=15&ncid=aolhom00030000000001)
From gayle.callis <@t> bresnan.net  Fri Mar 28 15:41:05 2008
From: gayle.callis <@t> bresnan.net (Gayle Callis)
Date: Fri Mar 28 15:41:08 2008
Subject: [Histonet] Hematoxylin Shortage??
References: <81a8916f0803271428m130df056s1e775ba17025736b@mail.gmail.com>
	
Message-ID: <006c01c89114$0b416e30$6401a8c0@DHXTS541>

Not the goat dying, but more like a boat sinking.

The last time there was a "rumored" hematoxylin shortage, back in the 70's, 
it was some boat sinking with all the Campechium logs aboard.  I recall some 
panic stockpiling of hematoxylin - the days when we made the stain ourselves 
with mercuric oxide.  I think the sunken boat rumor was a figment of 
someones vivid imagination.

For all the hematoxylin historians out there, correct me if I am wrong.

Gayle Callis
HTL/HT/MT(ASCP)
Bozeman MT 59715


----- Original Message ----- 
From: "Emily Sours" 
To: 
Sent: Friday, March 28, 2008 7:25 AM
Subject: Fwd: [Histonet] Hematoxylin Shortage??


> Why would this happen?
> Is this like that one goat dying at Santa Cruz?
>
> Emily
> -- 
> People aren't like chocolates. People are bastards. Bastards with
> bastard coating and bastard filling.
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


From gayle.callis <@t> bresnan.net  Fri Mar 28 16:08:47 2008
From: gayle.callis <@t> bresnan.net (Gayle Callis)
Date: Fri Mar 28 16:08:51 2008
Subject: [Histonet] Immuno on decaled toenail????
References: 
Message-ID: <008401c89117$ea344ab0$6401a8c0@DHXTS541>

Toenails do not contain calcium, although an acid may perform some protein 
hydrolysis to break down the protein to may help soften the keratin.  This 
may also damage the antigenic epitope.  Some people use NAIR, or an alkaline 
solution to soften the keratin, beware, high pH also damages alkaline 
sensitive linkages too.

Histonet has many suggestions for softening toenails already, but mostly for 
sectioning purposes.  Check the archives and do a search.

Sounds like a difficult situation for this IHC.

Good luck

Gayle M. Callis
HTL/HT/MT(ASCP)
Bozeman MT 59715


---- Original Message ----- 
From: 
To: 
Sent: Friday, March 28, 2008 2:26 PM
Subject: [Histonet] Immuno on decaled toenail????


> Hello Histoland,
> Is there any techs that have performed an immuno stains such as S100 on a
> toenail?  Had any problems with staining after using a decal  solution?  I 
> have
> stained twice and pathologist is not happy with staining  pattern.  This 
> is
> the first nail that I've had to do a immunohistochemical  stain on to rule 
> out
> melanoma.  Any other advice on softening  toenail?  Sorry for all of the
> questions, but I know this is a great place  to start.  Thanks in advance.
>
> Sylinda Battle
>
>
>
>
> **************Create a Home Theater Like the Pros. Watch the video on AOL
> Home.
> (http://home.aol.com/diy/home-improvement-eric-stromer?video=15&ncid=aolhom00030000000001)
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


From darkdaym <@t> comcast.net  Fri Mar 28 18:32:32 2008
From: darkdaym <@t> comcast.net (Mark Ray)
Date: Fri Mar 28 17:32:16 2008
Subject: [Histonet] Hematoxylin Shortage
Message-ID: <47ED8010.7070000@comcast.net>

Dear Histonetters,

My company distributes Hematoxylin Powder and manufactures Hematoxylin 
Solutions for Histology.  We have reliable information regarding the 
Hematoxylin shortage.  All the world's crude Hematoxylin had been 
produced by a plant in Campeche, Mexico which has closed.  One of the  
major producers of purified Hematoxylin is trying to take up the slack 
by importing Logwood logs and extracting the dye at its plant in another 
country.  Of course shipping whole logs is much more expensive than 
shipping crude Hematoxylin, so the cost of production has risen and less 
dye is being produced.   Be prepared for higher prices and shortages.  
It appears that crude Hematoxylin production will soon begin again in 
Campeche. This should lead to lower prices and increased supplies within 
about a year.

Mark Ray
E K Industries

From marktarango <@t> gmail.com  Fri Mar 28 18:10:39 2008
From: marktarango <@t> gmail.com (Mark Tarango)
Date: Fri Mar 28 18:10:42 2008
Subject: [Histonet] Hematoxylin Shortage
In-Reply-To: <47ED8010.7070000@comcast.net>
References: <47ED8010.7070000@comcast.net>
Message-ID: <5b6eb13e0803281610o52ed81eavffc6455d9c461bc4@mail.gmail.com>

I hope they learned from their mistakes and don't stick all the logs on one
boat for export.

On Fri, Mar 28, 2008 at 4:32 PM, Mark Ray  wrote:

> Dear Histonetters,
>
> My company distributes Hematoxylin Powder and manufactures Hematoxylin
> Solutions for Histology.  We have reliable information regarding the
> Hematoxylin shortage.  All the world's crude Hematoxylin had been
> produced by a plant in Campeche, Mexico which has closed.  One of the
> major producers of purified Hematoxylin is trying to take up the slack
> by importing Logwood logs and extracting the dye at its plant in another
> country.  Of course shipping whole logs is much more expensive than
> shipping crude Hematoxylin, so the cost of production has risen and less
> dye is being produced.   Be prepared for higher prices and shortages.
> It appears that crude Hematoxylin production will soon begin again in
> Campeche. This should lead to lower prices and increased supplies within
> about a year.
>
> Mark Ray
> E K Industries
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
From cytologer <@t> msn.com  Sat Mar 29 00:23:21 2008
From: cytologer <@t> msn.com (ChoiUl Soo)
Date: Sat Mar 29 00:23:26 2008
Subject: [Histonet] Anti-MIP 1a antibody, anti - RANKL antibody
Message-ID: 

Hello histonetters! Always thanks to your kind feedbacks on my questions, and still I have one question..... I am wondering if anyone has experience with anti MIP-1a and RANKL antibody to canine tissues.  Greatly appreciated if you locate me ones which are cross reactive to dog FFPE tissues.  Thank you,  Regards,  Ul Soo Choi, DVM, PhDHaemeru Ltd. (Haemaru Referral Animal Hospital)272-5 Veteterinary Science Bld., Seo Hyun dong, Bun Dang gu, Sung Nam city,Kyung Gi do, Korea 463-050TEL 82-31-781-2992FAX 82-31-781 2993 
_________________________________________________________________
MSN ???? ??? ??, Windows Live Messenger!
http://windowslive.msn.co.kr/wlm/messenger/
From rjbuesa <@t> yahoo.com  Sat Mar 29 09:57:21 2008
From: rjbuesa <@t> yahoo.com (Rene J Buesa)
Date: Sat Mar 29 09:57:25 2008
Subject: [Histonet] Immuno on decaled toenail????
In-Reply-To: 
Message-ID: <399687.24946.qm@web65708.mail.ac4.yahoo.com>

First toenails do not contain calcium and the keratin they are made does not respond adequately to acid hydrolysis. You will need an alkaline hydrolysis (10% NaOH for at least 30 minutes after fixation before processing).
  After that the IHC is likely to be weak and you will need some twinkling.
  Ren? J.

BSylinda@aol.com wrote:
  Hello Histoland,
Is there any techs that have performed an immuno stains such as S100 on a 
toenail? Had any problems with staining after using a decal solution? I have 
stained twice and pathologist is not happy with staining pattern. This is 
the first nail that I've had to do a immunohistochemical stain on to rule out 
melanoma. Any other advice on softening toenail? Sorry for all of the 
questions, but I know this is a great place to start. Thanks in advance.

Sylinda Battle




**************Create a Home Theater Like the Pros. Watch the video on AOL 
Home. 
(http://home.aol.com/diy/home-improvement-eric-stromer?video=15&ncid=aolhom00030000000001)
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


       
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From barbaraaalbert <@t> yahoo.com  Sat Mar 29 11:09:46 2008
From: barbaraaalbert <@t> yahoo.com (Barbara Albert)
Date: Sat Mar 29 11:09:50 2008
Subject: [Histonet] Hematoxylin Shortage??
In-Reply-To: <006c01c89114$0b416e30$6401a8c0@DHXTS541>
Message-ID: <514289.86390.qm@web63705.mail.re1.yahoo.com>

Gayle,
  I can't correct you because I know even fewer facts, but the feeling around our part of the world at that time was that vendors were buying up powder so that we would be "forced" to purchse their ready-made product.  I guess we urbanites are known for our cynical world view.
   
  Barbara Albert
  UCSF Medical Center
  San Francisco, CA

Gayle Callis  wrote:
  Not the goat dying, but more like a boat sinking.

The last time there was a "rumored" hematoxylin shortage, back in the 70's, 
it was some boat sinking with all the Campechium logs aboard. I recall some 
panic stockpiling of hematoxylin - the days when we made the stain ourselves 
with mercuric oxide. I think the sunken boat rumor was a figment of 
someones vivid imagination.

For all the hematoxylin historians out there, correct me if I am wrong.

Gayle Callis
HTL/HT/MT(ASCP)
Bozeman MT 59715


----- Original Message ----- 
From: "Emily Sours" 
To: 
Sent: Friday, March 28, 2008 7:25 AM
Subject: Fwd: [Histonet] Hematoxylin Shortage??


> Why would this happen?
> Is this like that one goat dying at Santa Cruz?
>
> Emily
> -- 
> People aren't like chocolates. People are bastards. Bastards with
> bastard coating and bastard filling.
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


       
---------------------------------
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From gayle.callis <@t> bresnan.net  Sat Mar 29 12:22:28 2008
From: gayle.callis <@t> bresnan.net (Gayle Callis)
Date: Sat Mar 29 12:22:31 2008
Subject: [Histonet] Hematoxylin Shortage
References: <47ED8010.7070000@comcast.net>
Message-ID: <000b01c891c1$76d1b670$6401a8c0@DHXTS541>

Mark,

Then what we supposed to do?  Stockpile commercial the popular commercial 
mixtures, and let them outdate on the shelf?  Maybe we need to go back to 
making our own solutions, with dry powders.   I would assume higher prices 
from shippers may bemay be more linked to shi


----- Original Message ----- 
From: "Mark Ray" 
To: 
Sent: Friday, March 28, 2008 5:32 PM
Subject: [Histonet] Hematoxylin Shortage


> Dear Histonetters,
>
> My company distributes Hematoxylin Powder and manufactures Hematoxylin 
> Solutions for Histology.  We have reliable information regarding the 
> Hematoxylin shortage.  All the world's crude Hematoxylin had been produced 
> by a plant in Campeche, Mexico which has closed.  One of the  major 
> producers of purified Hematoxylin is trying to take up the slack by 
> importing Logwood logs and extracting the dye at its plant in another 
> country.  Of course shipping whole logs is much more expensive than 
> shipping crude Hematoxylin, so the cost of production has risen and less 
> dye is being produced.   Be prepared for higher prices and shortages.  It 
> appears that crude Hematoxylin production will soon begin again in 
> Campeche. This should lead to lower prices and increased supplies within 
> about a year.
>
> Mark Ray
> E K Industries
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


From gayle.callis <@t> bresnan.net  Sat Mar 29 12:27:21 2008
From: gayle.callis <@t> bresnan.net (Gayle Callis)
Date: Sat Mar 29 12:27:22 2008
Subject: [Histonet] Hematoxylin Shortage
Message-ID: <001701c891c2$252fc630$6401a8c0@DHXTS541>

Sorry I hit the send button too soon.

I wrote:

Mark,

Then what we supposed to do?  Stockpile commercial the popular commercial 
mixtures, and let them outdate on the shelf?  Not a comforting thought for 
those who have to buy in bulk due to large volumes.  Maybe we need to go 
back to making our own solutions, with dry powders.  I would assume some of 
the higher prices will come from shippers, linked to high oil prices.  What 
a muddle.

Gayle Callis



----- Original Message ----- 
From: "Gayle Callis" 
To: "Mark Ray" ; 
Sent: Saturday, March 29, 2008 11:22 AM
Subject: Re: [Histonet] Hematoxylin Shortage


> Mark,
>
> Then what we supposed to do?  Stockpile commercial the popular commercial 
> mixtures, and let them outdate on the shelf?  Maybe we need to go back to 
> making our own solutions, with dry powders.   I would assume higher prices 
> from shippers may bemay be more linked to shi
>
>
> ----- Original Message ----- 
> From: "Mark Ray" 
> To: 
> Sent: Friday, March 28, 2008 5:32 PM
> Subject: [Histonet] Hematoxylin Shortage
>
>
>> Dear Histonetters,
>>
>> My company distributes Hematoxylin Powder and manufactures Hematoxylin 
>> Solutions for Histology.  We have reliable information regarding the 
>> Hematoxylin shortage.  All the world's crude Hematoxylin had been 
>> produced by a plant in Campeche, Mexico which has closed.  One of the 
>> major producers of purified Hematoxylin is trying to take up the slack by 
>> importing Logwood logs and extracting the dye at its plant in another 
>> country.  Of course shipping whole logs is much more expensive than 
>> shipping crude Hematoxylin, so the cost of production has risen and less 
>> dye is being produced.   Be prepared for higher prices and shortages.  It 
>> appears that crude Hematoxylin production will soon begin again in 
>> Campeche. This should lead to lower prices and increased supplies within 
>> about a year.
>>
>> Mark Ray
>> E K Industries
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 


From carl.hobbs <@t> kcl.ac.uk  Sat Mar 29 13:23:56 2008
From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs)
Date: Sat Mar 29 13:24:26 2008
Subject: [Histonet] Hematoxylin Shortage??
Message-ID: <002001c891ca$0cdf9620$4001a8c0@carlba65530bda>

Lol....I remember that time of Hx shortage, Gayle.
Scrambling around for alternatives, the papers published about 
alternatives.....
Why didn't the alternatives replace Hx?

NB: Yes, we heard and believed  that great story of the boat carrying them 
logs sinking at sea.....sadly, apocryphal.
Excessive forest clearance, I recall, was responsible?
Well, just checked on Wikipedia and that's the "story" they tell.....
Then read Mark Ray's post. Ah, a Hx processing plant in Campeche, Mexico!
Hence Haematoxylon Campechianum...... ;-)
A timely post for me as I am currently looking to revert to homemade Hx 
solns.( I work in a Research Centre with no Accreditation issues to worry 
about.)
A fascinating article here, on log/brazilwoods: 
http://waynesword.palomar.edu/ecoph4.htm
Carl 


From akemiat3377 <@t> yahoo.com  Sat Mar 29 14:55:02 2008
From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha)
Date: Sat Mar 29 14:55:05 2008
Subject: [Histonet] Hematoxylin Shortage??
In-Reply-To: <006c01c89114$0b416e30$6401a8c0@DHXTS541>
Message-ID: <301368.35051.qm@web31307.mail.mud.yahoo.com>

Hi All,

Yes, Gayle's memory is still going strong!  I remember
the boat rumor around 1976.  And yes, I too was making
Harris hematoxylin with mercuric oxide "red".  We
would let it ripen in the dark for a few weeks before
using it.  What a wonderful sheen it had.  I remember
a student was making it in the old Providence Hall
nursing school before we moved to our new lab.  The
student added the total amount of the mercuric oxide
and it shot straight up to the ceiling and permanently
stained it.   That was a heck of a lesson!

Regards,
Akemi

--- Gayle Callis  wrote:

> Not the goat dying, but more like a boat sinking.
> 
> The last time there was a "rumored" hematoxylin
> shortage, back in the 70's, 
> it was some boat sinking with all the Campechium
> logs aboard.  I recall some 
> panic stockpiling of hematoxylin - the days when we
> made the stain ourselves 
> with mercuric oxide.  I think the sunken boat rumor
> was a figment of 
> someones vivid imagination.
> 
> For all the hematoxylin historians out there,
> correct me if I am wrong.
> 
> Gayle Callis
> HTL/HT/MT(ASCP)
> Bozeman MT 59715
> 
> 
> ----- Original Message ----- 
> From: "Emily Sours" 
> To: 
> Sent: Friday, March 28, 2008 7:25 AM
> Subject: Fwd: [Histonet] Hematoxylin Shortage??
> 
> 
> > Why would this happen?
> > Is this like that one goat dying at Santa Cruz?
> >
> > Emily
> > -- 
> > People aren't like chocolates. People are
> bastards. Bastards with
> > bastard coating and bastard filling.
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> >
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 


Akemi Allison-Tacha, BS, HT(ASCP)HTL
Client Services Manager
PhenoPath laboratories
551 North 34th Street, Suite 100 
Seattle, WA 98103-8675
Work: (206) 374-9000
Cell: (425) 941-4287
E-Mail: akemiat3377@yahoo.com

From jnocito <@t> satx.rr.com  Sat Mar 29 19:50:55 2008
From: jnocito <@t> satx.rr.com (Joe Nocito)
Date: Sat Mar 29 19:50:06 2008
Subject: [Histonet] Immuno on decaled toenail????
References: 
Message-ID: <009a01c89200$1d2ca6e0$0302a8c0@yourxhtr8hvc4p>

This is a job for "Joe the Toe", hold on while I get my cape on.

OK, I'm back. Try soaking the block in 10% sodium hydroxide for about ten 
minutes, then put it back on ice.
May I suggest that you soak you toenails in 10% sodium hydroxide before 
processing? I leave my blocks in the solution for about an hour, followed by 
a water rinse before they go on the processor. We had a melanoma from a 
thumb on a 21 year old that we had to perform immunos on. First time I saw 
melanoma from a 21 year old and in a nail. The 10% NaOH did a really nice 
job and we were able to get really nice sections. Hope this helps.

JTT
----- Original Message ----- 
From: 
To: 
Sent: Friday, March 28, 2008 3:26 PM
Subject: [Histonet] Immuno on decaled toenail????


> Hello Histoland,
> Is there any techs that have performed an immuno stains such as S100 on a
> toenail?  Had any problems with staining after using a decal  solution?  I 
> have
> stained twice and pathologist is not happy with staining  pattern.  This 
> is
> the first nail that I've had to do a immunohistochemical  stain on to rule 
> out
> melanoma.  Any other advice on softening  toenail?  Sorry for all of the
> questions, but I know this is a great place  to start.  Thanks in advance.
>
> Sylinda Battle
>
>
>
>
> **************Create a Home Theater Like the Pros. Watch the video on AOL
> Home.
> (http://home.aol.com/diy/home-improvement-eric-stromer?video=15&ncid=aolhom00030000000001)
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


From jnocito <@t> satx.rr.com  Sat Mar 29 19:52:42 2008
From: jnocito <@t> satx.rr.com (Joe Nocito)
Date: Sat Mar 29 19:51:48 2008
Subject: [Histonet] Hematoxylin Shortage??
References: <81a8916f0803271428m130df056s1e775ba17025736b@mail.gmail.com>
	<006c01c89114$0b416e30$6401a8c0@DHXTS541>
Message-ID: <00a301c89200$5c6e0ab0$0302a8c0@yourxhtr8hvc4p>

I remember that Gayle. I don't remember the reason for the shortage, but I 
do remember stock-piling a bunch of hematoxylin.

JTT
----- Original Message ----- 
From: "Gayle Callis" 
To: "Emily Sours" ; 

Sent: Friday, March 28, 2008 3:41 PM
Subject: Re: [Histonet] Hematoxylin Shortage??


> Not the goat dying, but more like a boat sinking.
>
> The last time there was a "rumored" hematoxylin shortage, back in the 
> 70's, it was some boat sinking with all the Campechium logs aboard.  I 
> recall some panic stockpiling of hematoxylin - the days when we made the 
> stain ourselves with mercuric oxide.  I think the sunken boat rumor was a 
> figment of someones vivid imagination.
>
> For all the hematoxylin historians out there, correct me if I am wrong.
>
> Gayle Callis
> HTL/HT/MT(ASCP)
> Bozeman MT 59715
>
>
> ----- Original Message ----- 
> From: "Emily Sours" 
> To: 
> Sent: Friday, March 28, 2008 7:25 AM
> Subject: Fwd: [Histonet] Hematoxylin Shortage??
>
>
>> Why would this happen?
>> Is this like that one goat dying at Santa Cruz?
>>
>> Emily
>> -- 
>> People aren't like chocolates. People are bastards. Bastards with
>> bastard coating and bastard filling.
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


From jnocito <@t> satx.rr.com  Sat Mar 29 19:54:20 2008
From: jnocito <@t> satx.rr.com (Joe Nocito)
Date: Sat Mar 29 19:53:25 2008
Subject: [Histonet] Hematoxylin Shortage
References: <47ED8010.7070000@comcast.net>
Message-ID: <00ba01c89200$96d7a7b0$0302a8c0@yourxhtr8hvc4p>

sounds a lot like the oil crisis.

----- Original Message ----- 
From: "Mark Ray" 
To: 
Sent: Friday, March 28, 2008 6:32 PM
Subject: [Histonet] Hematoxylin Shortage


> Dear Histonetters,
> 
> My company distributes Hematoxylin Powder and manufactures Hematoxylin 
> Solutions for Histology.  We have reliable information regarding the 
> Hematoxylin shortage.  All the world's crude Hematoxylin had been 
> produced by a plant in Campeche, Mexico which has closed.  One of the  
> major producers of purified Hematoxylin is trying to take up the slack 
> by importing Logwood logs and extracting the dye at its plant in another 
> country.  Of course shipping whole logs is much more expensive than 
> shipping crude Hematoxylin, so the cost of production has risen and less 
> dye is being produced.   Be prepared for higher prices and shortages.  
> It appears that crude Hematoxylin production will soon begin again in 
> Campeche. This should lead to lower prices and increased supplies within 
> about a year.
> 
> Mark Ray
> E K Industries
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From jnocito <@t> satx.rr.com  Sat Mar 29 19:55:01 2008
From: jnocito <@t> satx.rr.com (Joe Nocito)
Date: Sat Mar 29 19:54:06 2008
Subject: [Histonet] Hematoxylin Shortage
References: <47ED8010.7070000@comcast.net>
	<5b6eb13e0803281610o52ed81eavffc6455d9c461bc4@mail.gmail.com>
Message-ID: <00c201c89200$afb09620$0302a8c0@yourxhtr8hvc4p>

is that something like "don't put all your eggs in one basket"
----- Original Message ----- 
From: "Mark Tarango" 
To: "Mark Ray" 
Cc: 
Sent: Friday, March 28, 2008 6:10 PM
Subject: Re: [Histonet] Hematoxylin Shortage


>I hope they learned from their mistakes and don't stick all the logs on one
> boat for export.
>
> On Fri, Mar 28, 2008 at 4:32 PM, Mark Ray  wrote:
>
>> Dear Histonetters,
>>
>> My company distributes Hematoxylin Powder and manufactures Hematoxylin
>> Solutions for Histology.  We have reliable information regarding the
>> Hematoxylin shortage.  All the world's crude Hematoxylin had been
>> produced by a plant in Campeche, Mexico which has closed.  One of the
>> major producers of purified Hematoxylin is trying to take up the slack
>> by importing Logwood logs and extracting the dye at its plant in another
>> country.  Of course shipping whole logs is much more expensive than
>> shipping crude Hematoxylin, so the cost of production has risen and less
>> dye is being produced.   Be prepared for higher prices and shortages.
>> It appears that crude Hematoxylin production will soon begin again in
>> Campeche. This should lead to lower prices and increased supplies within
>> about a year.
>>
>> Mark Ray
>> E K Industries
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


From kemlo <@t> f2s.com  Sun Mar 30 02:50:17 2008
From: kemlo <@t> f2s.com (kemlo)
Date: Sun Mar 30 02:51:57 2008
Subject: [Histonet] Hematoxylin Shortage
In-Reply-To: <001701c891c2$252fc630$6401a8c0@DHXTS541>
References: <001701c891c2$252fc630$6401a8c0@DHXTS541>
Message-ID: <7B5AE43869664F3394E1437D65D93955@KemloPC>

I remember the 'shortage' in the UK too, but we were still making our own
and therefore we weren't affected.

Never understood if there was a 'shortage' why the dry powder was still
available. Why didn't they use it to make the haematoxylin? Anyway logs
float don't they?

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis
Sent: 29 March 2008 17:27
To: Gayle Callis; Mark Ray; histonet@pathology.swmed.edu
Subject: Re: [Histonet] Hematoxylin Shortage

Sorry I hit the send button too soon.

I wrote:

Mark,

Then what we supposed to do?  Stockpile commercial the popular commercial 
mixtures, and let them outdate on the shelf?  Not a comforting thought for 
those who have to buy in bulk due to large volumes.  Maybe we need to go 
back to making our own solutions, with dry powders.  I would assume some of 
the higher prices will come from shippers, linked to high oil prices.  What 
a muddle.

Gayle Callis



----- Original Message ----- 
From: "Gayle Callis" 
To: "Mark Ray" ; 
Sent: Saturday, March 29, 2008 11:22 AM
Subject: Re: [Histonet] Hematoxylin Shortage


> Mark,
>
> Then what we supposed to do?  Stockpile commercial the popular commercial 
> mixtures, and let them outdate on the shelf?  Maybe we need to go back to 
> making our own solutions, with dry powders.   I would assume higher prices

> from shippers may bemay be more linked to shi
>
>
> ----- Original Message ----- 
> From: "Mark Ray" 
> To: 
> Sent: Friday, March 28, 2008 5:32 PM
> Subject: [Histonet] Hematoxylin Shortage
>
>
>> Dear Histonetters,
>>
>> My company distributes Hematoxylin Powder and manufactures Hematoxylin 
>> Solutions for Histology.  We have reliable information regarding the 
>> Hematoxylin shortage.  All the world's crude Hematoxylin had been 
>> produced by a plant in Campeche, Mexico which has closed.  One of the 
>> major producers of purified Hematoxylin is trying to take up the slack by

>> importing Logwood logs and extracting the dye at its plant in another 
>> country.  Of course shipping whole logs is much more expensive than 
>> shipping crude Hematoxylin, so the cost of production has risen and less 
>> dye is being produced.   Be prepared for higher prices and shortages.  It

>> appears that crude Hematoxylin production will soon begin again in 
>> Campeche. This should lead to lower prices and increased supplies within 
>> about a year.
>>
>> Mark Ray
>> E K Industries
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From kemlo <@t> f2s.com  Sun Mar 30 02:54:17 2008
From: kemlo <@t> f2s.com (kemlo)
Date: Sun Mar 30 02:55:51 2008
Subject: [Histonet] Hematoxylin Shortage??
In-Reply-To: <301368.35051.qm@web31307.mail.mud.yahoo.com>
References: <006c01c89114$0b416e30$6401a8c0@DHXTS541>
	<301368.35051.qm@web31307.mail.mud.yahoo.com>
Message-ID: 

We used to have 5 litre flasks of haematoxylin ripening in the window for
months; if you got taken short you had to artificially ripen using an
oxidising agent. If you had enough stock then the sun did the work.

I always thought "organic" haematoxylin stained better that the factory made
stuff but I do think it overoxidised at a slower rate and gave 'cleaner;
results.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi
Allison-Tacha
Sent: 29 March 2008 19:55
To: Gayle Callis; Emily Sours; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Hematoxylin Shortage??

Hi All,

Yes, Gayle's memory is still going strong!  I remember
the boat rumor around 1976.  And yes, I too was making
Harris hematoxylin with mercuric oxide "red".  We
would let it ripen in the dark for a few weeks before
using it.  What a wonderful sheen it had.  I remember
a student was making it in the old Providence Hall
nursing school before we moved to our new lab.  The
student added the total amount of the mercuric oxide
and it shot straight up to the ceiling and permanently
stained it.   That was a heck of a lesson!

Regards,
Akemi

--- Gayle Callis  wrote:

> Not the goat dying, but more like a boat sinking.
> 
> The last time there was a "rumored" hematoxylin
> shortage, back in the 70's, 
> it was some boat sinking with all the Campechium
> logs aboard.  I recall some 
> panic stockpiling of hematoxylin - the days when we
> made the stain ourselves 
> with mercuric oxide.  I think the sunken boat rumor
> was a figment of 
> someones vivid imagination.
> 
> For all the hematoxylin historians out there,
> correct me if I am wrong.
> 
> Gayle Callis
> HTL/HT/MT(ASCP)
> Bozeman MT 59715
> 
> 
> ----- Original Message ----- 
> From: "Emily Sours" 
> To: 
> Sent: Friday, March 28, 2008 7:25 AM
> Subject: Fwd: [Histonet] Hematoxylin Shortage??
> 
> 
> > Why would this happen?
> > Is this like that one goat dying at Santa Cruz?
> >
> > Emily
> > -- 
> > People aren't like chocolates. People are
> bastards. Bastards with
> > bastard coating and bastard filling.
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> >
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 


Akemi Allison-Tacha, BS, HT(ASCP)HTL
Client Services Manager
PhenoPath laboratories
551 North 34th Street, Suite 100 
Seattle, WA 98103-8675
Work: (206) 374-9000
Cell: (425) 941-4287
E-Mail: akemiat3377@yahoo.com

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From Barry.R.Rittman <@t> uth.tmc.edu  Sun Mar 30 07:53:48 2008
From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R)
Date: Sun Mar 30 07:53:53 2008
Subject: [Histonet] Hematoxylin Shortage??
References: <006c01c89114$0b416e30$6401a8c0@DHXTS541>
	<301368.35051.qm@web31307.mail.mud.yahoo.com>
	
Message-ID: 

I also think that home made hematoxylin such as Ehrlich's is superior in it's staining.
I have heard some criticism of controlling the oxidation so that the hematoxylin does not overoxidise. We used to have several bottles and when one had reached its "peak" then transfer to a large separating funnel with a layer of paraffin oil on the surface. This prevented further oxidation and could just dispense amount needed from the stopcock at the base.
I was trained in the UK where cost was always a factor and labor used to be cheaper.
Here in the States it seems as if almost everyone relies on made up solutions. This has two problems. One is that individuals may (and I repeat may) not realize why there are certain components in solutions and  not as readily appreciate the basis of the staining and therefore able to correct problems when they arise.
I believe that the decision to eliminate the practical portion of the HT exam pushes us further in that direction.
When my granddaughter (who is almost 4) is able to prepare a meal for us on her own she will have received a lot of instruction in preparing dishes from scratch.
Barry

________________________________

From: histonet-bounces@lists.utsouthwestern.edu on behalf of kemlo
Sent: Sun 3/30/2008 2:54 AM
To: 'Akemi Allison-Tacha'; 'Gayle Callis'; 'Emily Sours'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Hematoxylin Shortage??



We used to have 5 litre flasks of haematoxylin ripening in the window for
months; if you got taken short you had to artificially ripen using an
oxidising agent. If you had enough stock then the sun did the work.

I always thought "organic" haematoxylin stained better that the factory made
stuff but I do think it overoxidised at a slower rate and gave 'cleaner;
results.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi
Allison-Tacha
Sent: 29 March 2008 19:55
To: Gayle Callis; Emily Sours; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Hematoxylin Shortage??

Hi All,

Yes, Gayle's memory is still going strong!  I remember
the boat rumor around 1976.  And yes, I too was making
Harris hematoxylin with mercuric oxide "red".  We
would let it ripen in the dark for a few weeks before
using it.  What a wonderful sheen it had.  I remember
a student was making it in the old Providence Hall
nursing school before we moved to our new lab.  The
student added the total amount of the mercuric oxide
and it shot straight up to the ceiling and permanently
stained it.   That was a heck of a lesson!

Regards,
Akemi

--- Gayle Callis  wrote:

> Not the goat dying, but more like a boat sinking.
>
> The last time there was a "rumored" hematoxylin
> shortage, back in the 70's,
> it was some boat sinking with all the Campechium
> logs aboard.  I recall some
> panic stockpiling of hematoxylin - the days when we
> made the stain ourselves
> with mercuric oxide.  I think the sunken boat rumor
> was a figment of
> someones vivid imagination.
>
> For all the hematoxylin historians out there,
> correct me if I am wrong.
>
> Gayle Callis
> HTL/HT/MT(ASCP)
> Bozeman MT 59715
>
>
> ----- Original Message -----
> From: "Emily Sours" 
> To: 
> Sent: Friday, March 28, 2008 7:25 AM
> Subject: Fwd: [Histonet] Hematoxylin Shortage??
>
>
> > Why would this happen?
> > Is this like that one goat dying at Santa Cruz?
> >
> > Emily
> > --
> > People aren't like chocolates. People are
> bastards. Bastards with
> > bastard coating and bastard filling.
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> >
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>


Akemi Allison-Tacha, BS, HT(ASCP)HTL
Client Services Manager
PhenoPath laboratories
551 North 34th Street, Suite 100
Seattle, WA 98103-8675
Work: (206) 374-9000
Cell: (425) 941-4287
E-Mail: akemiat3377@yahoo.com

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



From jnocito <@t> satx.rr.com  Sun Mar 30 09:08:00 2008
From: jnocito <@t> satx.rr.com (Joe Nocito)
Date: Sun Mar 30 09:08:06 2008
Subject: [Histonet] Hematoxylin Shortage??
References: <006c01c89114$0b416e30$6401a8c0@DHXTS541><301368.35051.qm@web31307.mail.mud.yahoo.com>
	
Message-ID: <003101c8926f$770328a0$0302a8c0@yourxhtr8hvc4p>

I agree,
nothing like home made, that includes pies, cakes, lasagna. Sorry, went off 
on a tangent.

JTT
----- Original Message ----- 
From: "kemlo" 
To: "'Akemi Allison-Tacha'" ; "'Gayle Callis'" 
; "'Emily Sours'" ; 

Sent: Sunday, March 30, 2008 2:54 AM
Subject: RE: [Histonet] Hematoxylin Shortage??


> We used to have 5 litre flasks of haematoxylin ripening in the window for
> months; if you got taken short you had to artificially ripen using an
> oxidising agent. If you had enough stock then the sun did the work.
>
> I always thought "organic" haematoxylin stained better that the factory 
> made
> stuff but I do think it overoxidised at a slower rate and gave 'cleaner;
> results.
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi
> Allison-Tacha
> Sent: 29 March 2008 19:55
> To: Gayle Callis; Emily Sours; histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Hematoxylin Shortage??
>
> Hi All,
>
> Yes, Gayle's memory is still going strong!  I remember
> the boat rumor around 1976.  And yes, I too was making
> Harris hematoxylin with mercuric oxide "red".  We
> would let it ripen in the dark for a few weeks before
> using it.  What a wonderful sheen it had.  I remember
> a student was making it in the old Providence Hall
> nursing school before we moved to our new lab.  The
> student added the total amount of the mercuric oxide
> and it shot straight up to the ceiling and permanently
> stained it.   That was a heck of a lesson!
>
> Regards,
> Akemi
>
> --- Gayle Callis  wrote:
>
>> Not the goat dying, but more like a boat sinking.
>>
>> The last time there was a "rumored" hematoxylin
>> shortage, back in the 70's,
>> it was some boat sinking with all the Campechium
>> logs aboard.  I recall some
>> panic stockpiling of hematoxylin - the days when we
>> made the stain ourselves
>> with mercuric oxide.  I think the sunken boat rumor
>> was a figment of
>> someones vivid imagination.
>>
>> For all the hematoxylin historians out there,
>> correct me if I am wrong.
>>
>> Gayle Callis
>> HTL/HT/MT(ASCP)
>> Bozeman MT 59715
>>
>>
>> ----- Original Message ----- 
>> From: "Emily Sours" 
>> To: 
>> Sent: Friday, March 28, 2008 7:25 AM
>> Subject: Fwd: [Histonet] Hematoxylin Shortage??
>>
>>
>> > Why would this happen?
>> > Is this like that one goat dying at Santa Cruz?
>> >
>> > Emily
>> > -- 
>> > People aren't like chocolates. People are
>> bastards. Bastards with
>> > bastard coating and bastard filling.
>> >
>> > _______________________________________________
>> > Histonet mailing list
>> > Histonet@lists.utsouthwestern.edu
>> >
>>
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>>
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
> Akemi Allison-Tacha, BS, HT(ASCP)HTL
> Client Services Manager
> PhenoPath laboratories
> 551 North 34th Street, Suite 100
> Seattle, WA 98103-8675
> Work: (206) 374-9000
> Cell: (425) 941-4287
> E-Mail: akemiat3377@yahoo.com
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


From carl.hobbs <@t> kcl.ac.uk  Sun Mar 30 12:30:42 2008
From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs)
Date: Sun Mar 30 12:31:00 2008
Subject: [Histonet] Hematoxylin Shortage??
Message-ID: <004401c8928b$c7cd9e70$4001a8c0@carlba65530bda>

kemlo....
No such thang as "organic" Haemalum....lol
I personally am damn glad those horrible days of pontificating Histologists 
are over.
I recall a meeting with Eric Wallington ( A God to us UK Histologists) when 
I was giving a talk about MOVING on...
I presented DPX as a BEST mounting cement. That man dissed me. massive!
He buried me, to the amusement of the audience..." nothing can replace 
Canada Balsam"
However, I have been proved right.
C


From Barry.R.Rittman <@t> uth.tmc.edu  Sun Mar 30 12:48:28 2008
From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R)
Date: Sun Mar 30 12:52:00 2008
Subject: [Histonet] DPX Canada balsam
References: <004401c8928b$c7cd9e70$4001a8c0@carlba65530bda>
Message-ID: 

Carl
unfortunately I think that you are both correct.
While I like DPX for routine work, there are some applications such as mounting ground sections for which I feel Canada Balsam is better.
Barry

________________________________

From: histonet-bounces@lists.utsouthwestern.edu on behalf of Carl Hobbs
Sent: Sun 3/30/2008 12:30 PM
To: Histonet
Subject: RE: [Histonet] Hematoxylin Shortage??



kemlo....
No such thang as "organic" Haemalum....lol
I personally am damn glad those horrible days of pontificating Histologists
are over.
I recall a meeting with Eric Wallington ( A God to us UK Histologists) when
I was giving a talk about MOVING on...
I presented DPX as a BEST mounting cement. That man dissed me. massive!
He buried me, to the amusement of the audience..." nothing can replace
Canada Balsam"
However, I have been proved right.
C


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Histonet mailing list
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From mickie25 <@t> netzero.net  Sun Mar 30 13:29:44 2008
From: mickie25 <@t> netzero.net (mickie25@netzero.net)
Date: Sun Mar 30 13:30:31 2008
Subject: [Histonet] Remembrances
Message-ID: <20080330.142944.4340.0@webmail01.dca.untd.com>

Carl,
 
Interesting to hear your remembrance. Brought back memories to me. I had the same thing happen at an NSH meeting in San Francisco in I believe it was 1974 or 1975. I was the first speaker at the meeting (terrified to be speaking in front of 500 people) and gave a talk about differentiating pigments using histochemical stains. The neat thing about that experience is when I finished I went and sat down next to a very nice lady who offered me a piece of candy and was very complimentary of 'all the young people bringing new knowledge to us'. That was Dezna Sheehan and I never forgot her, but frankly don't remember the person who made fun of my stumble on the podium. 
 
Three cheers for all the 'young' people bring advancement to our field!
 
Best Regards,
 
Mickie
 
Mickie Johnson, B.S., HTL(ASCP)
Mohs Histology Consulting Services, LLC
  & Mohs Lab Staffing
2507 S. Manito Blvd.
Spokane, WA 99203
509-954-7134
FAX   509-624-3926
Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com 
Email: mickie25@netzero.net
 
 
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carl Hobbs
Sent: Sunday, March 30, 2008 10:31 AM
To: Histonet
Subject: RE: [Histonet] Hematoxylin Shortage??
 
kemlo....
No such thang as "organic" Haemalum....lol
I personally am damn glad those horrible days of pontificating Histologists 
are over.
I recall a meeting with Eric Wallington ( A God to us UK Histologists) when 
I was giving a talk about MOVING on...
I presented DPX as a BEST mounting cement. That man dissed me. massive!
He buried me, to the amusement of the audience..." nothing can replace 
Canada Balsam"
However, I have been proved right.
C
 
 
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
From sjchtascp <@t> yahoo.com  Sun Mar 30 23:40:34 2008
From: sjchtascp <@t> yahoo.com (Steven Coakley)
Date: Sun Mar 30 23:40:38 2008
Subject: [Histonet] Hematoxylin Shortage.
Message-ID: <82682.53340.qm@web38206.mail.mud.yahoo.com>

Concord grape juce and frozen blueberrys 50/50.  Blend on high for 2 minutes.  Strain, and bring to RT.  Stain progessively for 3-5 minted, rince ibriefly in lukearm water then blue in scotts or a weak bluing agent.  If that doesn't work chill and use over ice cream.  win win solution.
   
  :-)

       
---------------------------------
No Cost - Get a month of Blockbuster Total Access now. Sweet deal for Yahoo! users and friends.
From laurie.reilly <@t> jcu.edu.au  Mon Mar 31 00:40:06 2008
From: laurie.reilly <@t> jcu.edu.au (Laurie Reilly)
Date: Mon Mar 31 00:41:56 2008
Subject: [Histonet] Hematoxylin Shortage.
In-Reply-To: <82682.53340.qm@web38206.mail.mud.yahoo.com>
References: <82682.53340.qm@web38206.mail.mud.yahoo.com>
Message-ID: <000c01c892f1$acf26700$5255db89@health.ad.jcu.edu.au>

Mulberry juice, with a mordant, also stains nuclei. (and anything else it
touches.)

Mr. Laurie REILLY
Histopathology
School of  Veterinary and Biomedical Sciences
James Cook University
Townsville  Qld.  4811
Australia.

Phone 07 4781 4468

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven
Coakley
Sent: Monday, 31 March 2008 2:41 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Hematoxylin Shortage.

Concord grape juce and frozen blueberrys 50/50.  Blend on high for 2
minutes.  Strain, and bring to RT.  Stain progessively for 3-5 minted, rince
ibriefly in lukearm water then blue in scotts or a weak bluing agent.  If
that doesn't work chill and use over ice cream.  win win solution.
   
  :-)

       
---------------------------------
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users and friends.
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From kemlo <@t> f2s.com  Mon Mar 31 03:24:35 2008
From: kemlo <@t> f2s.com (kemlo)
Date: Mon Mar 31 03:25:11 2008
Subject: [Histonet] Hematoxylin Shortage??
In-Reply-To: <004401c8928b$c7cd9e70$4001a8c0@carlba65530bda>
References: <004401c8928b$c7cd9e70$4001a8c0@carlba65530bda>
Message-ID: <342F2AB61B814ADA9040F5FBA1117220@KemloPC>

Thought Haematoxylin solution had organic chemicals in it; it does, doesn't
it? Maybe your right, maybe we don't need to know what goes into to things
anymore; but maybe innovation may be the casualty.

I'm staggered when asking Band 7 specialist practitioners if they know
what's in something to find they haven't got a clue. That's all disciplines
not just Histology, Pharmacists still seem to know what's in their 
Concoctions.

Do people still make up Retic solution or is that bought in too?
 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carl Hobbs
Sent: 30 March 2008 18:31
To: Histonet
Subject: RE: [Histonet] Hematoxylin Shortage??

kemlo....
No such thang as "organic" Haemalum....lol
I personally am damn glad those horrible days of pontificating Histologists 
are over.
I recall a meeting with Eric Wallington ( A God to us UK Histologists) when 
I was giving a talk about MOVING on...
I presented DPX as a BEST mounting cement. That man dissed me. massive!
He buried me, to the amusement of the audience..." nothing can replace 
Canada Balsam"
However, I have been proved right.
C


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




From ian.montgomery <@t> bio.gla.ac.uk  Mon Mar 31 04:12:39 2008
From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery)
Date: Mon Mar 31 04:12:45 2008
Subject: FW: [Histonet] Hematoxylin Shortage??
Message-ID: <000601c8930f$5e456da0$6424d182@IBLS.GLA.AC.UK>

	Would I ever buy a pre-made stain, NEVER. I want to know what's in
my stains and that means making them yourself. Plus, where's the pleasure in
buying pre-made stains when they can be easily made yourself. Take great
care you don't de-skill your job with this easy option. 
Ian.
 
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kemlo
Sent: 31 March 2008 09:25
To: 'Carl Hobbs'; 'Histonet'
Subject: RE: [Histonet] Hematoxylin Shortage??

Thought Haematoxylin solution had organic chemicals in it; it does, doesn't
it? Maybe your right, maybe we don't need to know what goes into to things
anymore; but maybe innovation may be the casualty.

I'm staggered when asking Band 7 specialist practitioners if they know
what's in something to find they haven't got a clue. That's all disciplines
not just Histology, Pharmacists still seem to know what's in their 
Concoctions.

Do people still make up Retic solution or is that bought in too?
 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carl Hobbs
Sent: 30 March 2008 18:31
To: Histonet
Subject: RE: [Histonet] Hematoxylin Shortage??

kemlo....
No such thang as "organic" Haemalum....lol
I personally am damn glad those horrible days of pontificating Histologists 
are over.
I recall a meeting with Eric Wallington ( A God to us UK Histologists) when 
I was giving a talk about MOVING on...
I presented DPX as a BEST mounting cement. That man dissed me. massive!
He buried me, to the amusement of the audience..." nothing can replace 
Canada Balsam"
However, I have been proved right.
C


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


From kemlo <@t> f2s.com  Mon Mar 31 04:37:58 2008
From: kemlo <@t> f2s.com (kemlo)
Date: Mon Mar 31 04:38:40 2008
Subject: [Histonet] Hematoxylin Shortage??
In-Reply-To: <000601c8930f$5e456da0$6424d182@IBLS.GLA.AC.UK>
References: <000601c8930f$5e456da0$6424d182@IBLS.GLA.AC.UK>
Message-ID: <2B990CFE5973437A9B0E6890CB5D6B75@KemloPC>

But don't you get CE problems? Doesn't the Common Market insist that they
have a 'kite mark'? What about CPA?

I with you Ian but we're a minority. Used to really enjoy making up the Van
Gieson.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian
Montgomery
Sent: 31 March 2008 10:13
To: histonet@lists.utsouthwestern.edu
Subject: FW: [Histonet] Hematoxylin Shortage??

	Would I ever buy a pre-made stain, NEVER. I want to know what's in
my stains and that means making them yourself. Plus, where's the pleasure in
buying pre-made stains when they can be easily made yourself. Take great
care you don't de-skill your job with this easy option. 
Ian.
 
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kemlo
Sent: 31 March 2008 09:25
To: 'Carl Hobbs'; 'Histonet'
Subject: RE: [Histonet] Hematoxylin Shortage??

Thought Haematoxylin solution had organic chemicals in it; it does, doesn't
it? Maybe your right, maybe we don't need to know what goes into to things
anymore; but maybe innovation may be the casualty.

I'm staggered when asking Band 7 specialist practitioners if they know
what's in something to find they haven't got a clue. That's all disciplines
not just Histology, Pharmacists still seem to know what's in their 
Concoctions.

Do people still make up Retic solution or is that bought in too?
 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carl Hobbs
Sent: 30 March 2008 18:31
To: Histonet
Subject: RE: [Histonet] Hematoxylin Shortage??

kemlo....
No such thang as "organic" Haemalum....lol
I personally am damn glad those horrible days of pontificating Histologists 
are over.
I recall a meeting with Eric Wallington ( A God to us UK Histologists) when 
I was giving a talk about MOVING on...
I presented DPX as a BEST mounting cement. That man dissed me. massive!
He buried me, to the amusement of the audience..." nothing can replace 
Canada Balsam"
However, I have been proved right.
C


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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Histonet mailing list
Histonet@lists.utsouthwestern.edu
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From ian.montgomery <@t> bio.gla.ac.uk  Mon Mar 31 05:36:15 2008
From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery)
Date: Mon Mar 31 05:36:21 2008
Subject: FW: [Histonet] Hematoxylin Shortage??
Message-ID: <001b01c8931b$0c32e040$6424d182@IBLS.GLA.AC.UK>

	No problems. Ok, this is a University and I do what's best for
teaching and research.  


Dr. Ian Montgomery,
Histotechnology,
I.B.L.S. Support Unit,
Thomson Building,
University of Glasgow,
Glasgow,
G12 8QQ.
-----Original Message-----
From: kemlo [mailto:kemlo@f2s.com] 
Sent: 31 March 2008 10:38
To: 'Ian Montgomery'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Hematoxylin Shortage??

But don't you get CE problems? Doesn't the Common Market insist that they
have a 'kite mark'? What about CPA?

I with you Ian but we're a minority. Used to really enjoy making up the Van
Gieson.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian
Montgomery
Sent: 31 March 2008 10:13
To: histonet@lists.utsouthwestern.edu
Subject: FW: [Histonet] Hematoxylin Shortage??

	Would I ever buy a pre-made stain, NEVER. I want to know what's in
my stains and that means making them yourself. Plus, where's the pleasure in
buying pre-made stains when they can be easily made yourself. Take great
care you don't de-skill your job with this easy option. 
Ian.
 
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kemlo
Sent: 31 March 2008 09:25
To: 'Carl Hobbs'; 'Histonet'
Subject: RE: [Histonet] Hematoxylin Shortage??

Thought Haematoxylin solution had organic chemicals in it; it does, doesn't
it? Maybe your right, maybe we don't need to know what goes into to things
anymore; but maybe innovation may be the casualty.

I'm staggered when asking Band 7 specialist practitioners if they know
what's in something to find they haven't got a clue. That's all disciplines
not just Histology, Pharmacists still seem to know what's in their 
Concoctions.

Do people still make up Retic solution or is that bought in too?
 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carl Hobbs
Sent: 30 March 2008 18:31
To: Histonet
Subject: RE: [Histonet] Hematoxylin Shortage??

kemlo....
No such thang as "organic" Haemalum....lol
I personally am damn glad those horrible days of pontificating Histologists 
are over.
I recall a meeting with Eric Wallington ( A God to us UK Histologists) when 
I was giving a talk about MOVING on...
I presented DPX as a BEST mounting cement. That man dissed me. massive!
He buried me, to the amusement of the audience..." nothing can replace 
Canada Balsam"
However, I have been proved right.
C


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





From jnocito <@t> satx.rr.com  Mon Mar 31 06:05:07 2008
From: jnocito <@t> satx.rr.com (Joe Nocito)
Date: Mon Mar 31 06:05:19 2008
Subject: [Histonet] Hematoxylin Shortage.
References: <82682.53340.qm@web38206.mail.mud.yahoo.com>
Message-ID: <001201c8931f$149dbc10$0302a8c0@yourxhtr8hvc4p>

vodka works just as well. With the Grape juice/ blueberry mix. Not for 
staining. Well, maybe, if you drink enough.

JTT
----- Original Message ----- 
From: "Steven Coakley" 
To: 
Sent: Sunday, March 30, 2008 11:40 PM
Subject: [Histonet] Hematoxylin Shortage.


> Concord grape juce and frozen blueberrys 50/50.  Blend on high for 2 
> minutes.  Strain, and bring to RT.  Stain progessively for 3-5 minted, 
> rince ibriefly in lukearm water then blue in scotts or a weak bluing 
> agent.  If that doesn't work chill and use over ice cream.  win win 
> solution.
>
>  :-)
>
>
> ---------------------------------
> No Cost - Get a month of Blockbuster Total Access now. Sweet deal for 
> Yahoo! users and friends.
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


From jnocito <@t> satx.rr.com  Mon Mar 31 06:08:56 2008
From: jnocito <@t> satx.rr.com (Joe Nocito)
Date: Mon Mar 31 06:09:00 2008
Subject: [Histonet] Hematoxylin Shortage??
References: <000601c8930f$5e456da0$6424d182@IBLS.GLA.AC.UK>
	<2B990CFE5973437A9B0E6890CB5D6B75@KemloPC>
Message-ID: <003101c8931f$9cf8a7f0$0302a8c0@yourxhtr8hvc4p>

I remember making Mayer's Mucicarmine solution one.  I was heating it on a 
hot plate and wasn't watching it as closely as I should have. The solution 
exploded. The ceiling was a nice magenta color, as well as anything within a 
meter of the hot plate.

JTT
----- Original Message ----- 
From: "kemlo" 
To: "'Ian Montgomery'" ; 

Sent: Monday, March 31, 2008 4:37 AM
Subject: RE: [Histonet] Hematoxylin Shortage??


> But don't you get CE problems? Doesn't the Common Market insist that they
> have a 'kite mark'? What about CPA?
>
> I with you Ian but we're a minority. Used to really enjoy making up the 
> Van
> Gieson.
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian
> Montgomery
> Sent: 31 March 2008 10:13
> To: histonet@lists.utsouthwestern.edu
> Subject: FW: [Histonet] Hematoxylin Shortage??
>
> Would I ever buy a pre-made stain, NEVER. I want to know what's in
> my stains and that means making them yourself. Plus, where's the pleasure 
> in
> buying pre-made stains when they can be easily made yourself. Take great
> care you don't de-skill your job with this easy option.
> Ian.
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kemlo
> Sent: 31 March 2008 09:25
> To: 'Carl Hobbs'; 'Histonet'
> Subject: RE: [Histonet] Hematoxylin Shortage??
>
> Thought Haematoxylin solution had organic chemicals in it; it does, 
> doesn't
> it? Maybe your right, maybe we don't need to know what goes into to things
> anymore; but maybe innovation may be the casualty.
>
> I'm staggered when asking Band 7 specialist practitioners if they know
> what's in something to find they haven't got a clue. That's all 
> disciplines
> not just Histology, Pharmacists still seem to know what's in their
> Concoctions.
>
> Do people still make up Retic solution or is that bought in too?
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carl Hobbs
> Sent: 30 March 2008 18:31
> To: Histonet
> Subject: RE: [Histonet] Hematoxylin Shortage??
>
> kemlo....
> No such thang as "organic" Haemalum....lol
> I personally am damn glad those horrible days of pontificating 
> Histologists
> are over.
> I recall a meeting with Eric Wallington ( A God to us UK Histologists) 
> when
> I was giving a talk about MOVING on...
> I presented DPX as a BEST mounting cement. That man dissed me. massive!
> He buried me, to the amusement of the audience..." nothing can replace
> Canada Balsam"
> However, I have been proved right.
> C
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


From mickie25 <@t> netzero.net  Mon Mar 31 06:28:00 2008
From: mickie25 <@t> netzero.net (mickie25@netzero.net)
Date: Mon Mar 31 06:29:05 2008
Subject: [Histonet] Thanks. ? about Albuquerque
Message-ID: <20080331.072800.6226.1@webmail17.dca.untd.com>

Hi Susan
Thanks for the reply about Dr. F. Terry is preparing your pay check for GH and Dr. F as we speak. Don't worry and thanks for the details. This man is certifiable.
Regarding Albuquerque, are you OK with doing Mohs for up to 10-12 hours on Tuesday and then working out the rest of the days to make 40 hours? That would mean 12 or so cases on Tuesday and 6 on Wednesday with extra time on Wed, Thurs., and Friday for paraffin histology?
You would get paid as if you worked 5 8 hour days from our perspective, plus perdiem, etc.
I am assuming so. Let me know if you feel different. 
Thanks
Mickie


Mickie Johnson, B.S., HTL(ASCP)
Mohs Histology Consulting Services
2507 S. Manito Blvd.
Spokane, WA 99203
509-954-7134 www.mohshistologyconsulting.com
From sbreeden <@t> nmda.nmsu.edu  Mon Mar 31 07:04:43 2008
From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara)
Date: Mon Mar 31 07:04:51 2008
Subject: [Histonet] Hematoxylin Ripening
Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E6244@nmdamailsvr.nmda.ad.nmsu.edu>

Kemlo - I didn't know there was enough sun there to ripen hematoxylin on
a windowsill...  :-)

 

Sally Breeden, HT(ASCP)

NM Dept. of Agriculture

Veterinary Diagnostic Services

PO Box 4700

Albuquerque, NM  87106

505-841-2576

 

From GDawson <@t> dynacaremilwaukee.com  Mon Mar 31 08:22:51 2008
From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen)
Date: Mon Mar 31 08:23:02 2008
Subject: [Histonet] VEGF
In-Reply-To: <121674.98498.qm@web65707.mail.ac4.yahoo.com>
Message-ID: 

All,

I have a pathologist requesting a Vascular Endolthelial Growth Factor (VEGF) antibody.  Just wondering which one is favored out there in histoland as everybody and their sister seems to carry it.

Any input would be appreciated.

Thanx In Advance,

Glen Dawson  BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI

From vazquezr <@t> ohsu.edu  Mon Mar 31 08:36:27 2008
From: vazquezr <@t> ohsu.edu (Robyn Vazquez)
Date: Mon Mar 31 08:36:58 2008
Subject: [Histonet] Immuno on decaled toenail????
Message-ID: 

Joe,
Would this work for nose bone for a frozen section?
 
Robyn

>>> "Joe Nocito"  3/29/2008 5:50 PM >>>

This is a job for "Joe the Toe", hold on while I get my cape on.

OK, I'm back. Try soaking the block in 10% sodium hydroxide for about
ten 
minutes, then put it back on ice.
May I suggest that you soak you toenails in 10% sodium hydroxide before

processing? I leave my blocks in the solution for about an hour,
followed by 
a water rinse before they go on the processor. We had a melanoma from a

thumb on a 21 year old that we had to perform immunos on. First time I
saw 
melanoma from a 21 year old and in a nail. The 10% NaOH did a really
nice 
job and we were able to get really nice sections. Hope this helps.

JTT
----- Original Message ----- 
From: 
To: 
Sent: Friday, March 28, 2008 3:26 PM
Subject: [Histonet] Immuno on decaled toenail????


> Hello Histoland,
> Is there any techs that have performed an immuno stains such as S100
on a
> toenail?  Had any problems with staining after using a decal 
solution?  I 
> have
> stained twice and pathologist is not happy with staining  pattern. 
This 
> is
> the first nail that I've had to do a immunohistochemical  stain on to
rule 
> out
> melanoma.  Any other advice on softening  toenail?  Sorry for all of
the
> questions, but I know this is a great place  to start.  Thanks in
advance.
>
> Sylinda Battle
>
>
>
>
> **************Create a Home Theater Like the Pros. Watch the video on
AOL
> Home.
>
(http://home.aol.com/diy/home-improvement-eric-stromer?video=15&ncid=aolhom00030000000001)
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


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From vazquezr <@t> ohsu.edu  Mon Mar 31 08:44:35 2008
From: vazquezr <@t> ohsu.edu (Robyn Vazquez)
Date: Mon Mar 31 08:45:05 2008
Subject: [Histonet] Hematoxylin Shortage.
Message-ID: 

Steven,
That really works?  
 
Robyn

>>> "Steven Coakley"  3/30/2008 9:40 PM >>>

Concord grape juce and frozen blueberrys 50/50.  Blend on high for 2
minutes.  Strain, and bring to RT.  Stain progessively for 3-5 minted,
rince ibriefly in lukearm water then blue in scotts or a weak bluing
agent.  If that doesn't work chill and use over ice cream.  win win
solution.
   
  :-)

       
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From Terry.Marshall <@t> rothgen.nhs.uk  Mon Mar 31 09:00:07 2008
From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr,
	Consultant Histopathologist)
Date: Mon Mar 31 09:00:17 2008
Subject: [Histonet] Hematoxylin Shortage.
Message-ID: <5C0BED61F529364E86309CADEA63FEF20163F3AE@TRFT-EX01.xRothGen.nhs.uk>

It works as well as his spell check:-)

Terry 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robyn
Vazquez
Sent: 31 March 2008 14:45
To: Histonet@lists.utsouthwestern.edu; sjchtascp@yahoo.com
Subject: Re: [Histonet] Hematoxylin Shortage.

Steven,
That really works?  
 
Robyn

>>> "Steven Coakley"  3/30/2008 9:40 PM >>>

Concord grape juce and frozen blueberrys 50/50.  Blend on high for 2
minutes.  Strain, and bring to RT.  Stain progessively for 3-5 minted,
rince ibriefly in lukearm water then blue in scotts or a weak bluing
agent.  If that doesn't work chill and use over ice cream.  win win
solution.
   
  :-)

       
---------------------------------
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From vazquezr <@t> ohsu.edu  Mon Mar 31 09:18:21 2008
From: vazquezr <@t> ohsu.edu (Robyn Vazquez)
Date: Mon Mar 31 09:18:50 2008
Subject: [Histonet] Hematoxylin Shortage.
Message-ID: 

Terry/Steven,
Would the dessert be called blue/grape icecream camode?  )=  happy
Monday!
Robyn

>>> "Marshall Terry Dr, Consultant Histopathologist"
 3/31/2008 7:00:07 AM >>>

It works as well as his spell check:-)

Terry 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robyn
Vazquez
Sent: 31 March 2008 14:45
To: Histonet@lists.utsouthwestern.edu; sjchtascp@yahoo.com
Subject: Re: [Histonet] Hematoxylin Shortage.

Steven,
That really works?  

Robyn

>>> "Steven Coakley"  3/30/2008 9:40 PM >>>

Concord grape juce and frozen blueberrys 50/50.  Blend on high for 2
minutes.  Strain, and bring to RT.  Stain progessively for 3-5 minted,
rince ibriefly in lukearm water then blue in scotts or a weak bluing
agent.  If that doesn't work chill and use over ice cream.  win win
solution.
   
  :-)

       
---------------------------------
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Yahoo! users and friends.
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From jflinn <@t> gmu.edu  Mon Mar 31 10:52:22 2008
From: jflinn <@t> gmu.edu (Jane M Flinn)
Date: Mon Mar 31 10:52:53 2008
Subject: [Histonet] Re: cleaning rat teeth
In-Reply-To: <2914.65.40.219.161.1206147834.squirrel@host7.wfdns.com>
References: <47DE536C.C068.0078.0@hsc.wvu.edu> <47DE935C.8000803@umdnj.edu>
	<2914.65.40.219.161.1206147834.squirrel@host7.wfdns.com>
Message-ID: 

I have sent some rats' teeth to a friend for her research, as described below. The teeth have some tissue on them. What would be the best way to get rid of this without disturbing the zinc binding?  jane

Thank you very much for the details. We basically want to look at Zn coordination environments in the teeth appatite, as comparing to our synthetic Zn-doped hydroxylapatite. So I'm guessing the older animals might have better crystalline apatite? Anyway, I probably have to grind them up seperately and do some XRD to check on the crystallinity, as well as maybe H2O2 or chlorox treatment to get rid of the organics. Thanks a lot for getting the samples! 



"Life is short - make haste to be kind"

Dr. Jane Flinn
Director, Biopsychology Program
George Mason University, 3F5
4400 University Dr.
Fairfax, VA  22030
Phone: 703-993-4107
Fax: 703-993-1359


From MadaryJ <@t> MedImmune.com  Mon Mar 31 11:01:51 2008
From: MadaryJ <@t> MedImmune.com (Madary, Joseph)
Date: Mon Mar 31 11:02:11 2008
Subject: [Histonet] tbs shur mount glass coverslipper feedback? and the
	Hematoxylin comment
Message-ID: 

I am looking at glass coverslippers and found the Sakura was what many
folks liked, but the tbs is a cool 10K cheaper so I am wondering what
people think of that?  I recall the shortage too.  Actually  a few
motnsh ago I used Celestine blue as a counterstain for a PAS and I had
forgotten how great that looks, and how easy it is to make.

 

Nick Madary, HT/HTL(ASCP)QIHC

Histology Mgr, Medimmune

301.398.6360(lab), 4745(vm),9745(fax)

 

"To the extent this electronic communication or any of its attachments
contain information that is not in the public domain, such information
is considered by MedImmune to be confidential and proprietary, and
expected to be used only by the individual(s) for whom it is intended.
If you have received this electronic communication in error, please
reply to the sender advising of the error in transmission and delete the
original message and any accompanying documents from your system
immediately, without copying, reviewing or otherwise using them for any
purpose.  Thank you for your cooperation."

 

 




To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary.  This communication is expected to be read and/or used only by the individual(s) for whom it is intended.  If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose.  Thank you for your cooperation.
From MadaryJ <@t> MedImmune.com  Mon Mar 31 12:29:00 2008
From: MadaryJ <@t> MedImmune.com (Madary, Joseph)
Date: Mon Mar 31 12:30:08 2008
Subject: [Histonet] making vs commerical
Message-ID: 

I agree for the most part on home made.  I will say that for certain GLP
applications it is easier to have one lot of a reagent for documentation
purposes, otherwise you will have 7 different ingredients with
corresponding lot numbers.  I like making stuff up myself too if it
saves money or improves quality.  I have found that things like
mucicarmine and Schiffs reagent are a bit cheaper to buy.  If you have
made them enough times I doubt that you will ever be "de-skilled".

 

Nick Madary, HT/HTL(ASCP)QIHC

Histology Mgr, Medimmune

301.398.6360(lab), 4745(vm),9745(fax)

 

"To the extent this electronic communication or any of its attachments
contain information that is not in the public domain, such information
is considered by MedImmune to be confidential and proprietary, and
expected to be used only by the individual(s) for whom it is intended.
If you have received this electronic communication in error, please
reply to the sender advising of the error in transmission and delete the
original message and any accompanying documents from your system
immediately, without copying, reviewing or otherwise using them for any
purpose.  Thank you for your cooperation."

 

 




To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary.  This communication is expected to be read and/or used only by the individual(s) for whom it is intended.  If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose.  Thank you for your cooperation.
From RSRICHMOND <@t> aol.com  Mon Mar 31 13:07:21 2008
From: RSRICHMOND <@t> aol.com (Robert Richmond)
Date: Mon Mar 31 13:07:28 2008
Subject: [Histonet] Re: DPX Canada balsam
Message-ID: 

Sheesh, I'm nearly 70 and I barely remember Canada balsam! It's a
wonderfully sweet-smelling resin. Lillie described it as almost pure
abietic acid. It would destroy most stains within a few years -
neutralization of the balsam with potassium carbonate was said to
prevent this, though I don't see how.

Canada balsam took at least a month to set solidly enough that you
could file the slides. This "curing" could be speeded up by putting
the slides in flat trays in a 37 degree C incubator for a week. At
Johns Hopkins an ancient walk-in bacteriology incubator was still in
use - I loved to go in there because it smelled so nice. The synthetic
mounting medium in use around 1965 also benefited from a few days'
curing, so the incubator remained in use.

Around 1970 I took the coverslip off a 1932 Levaditi silver stain of
syphilitic infant liver, so I could replace it with a coverslip thin
enough for me to photograph the little black spirochetes under an oil
immersion objective. Took a week in warm xylene to get the thing to
fall off.

Canada balsam is still around - look at a can of orange soda and
you'll see it contains "glycerol ester of wood rosin". Yummers!

Bob Richmond
Samurai Pathologist
Knoxville TN

From sjchtascp <@t> yahoo.com  Mon Mar 31 13:50:20 2008
From: sjchtascp <@t> yahoo.com (Steven Coakley)
Date: Mon Mar 31 13:50:23 2008
Subject: [Histonet] Blackballed
Message-ID: <544090.42586.qm@web38202.mail.mud.yahoo.com>

Well, I suppose I must accept that a certain Lab director has long ago blackballed me from working in Histology, at least, in my area.  Guess that's what one gets from rebelling against being so stressed out from excessive periods of short staffing and temps.  Working long hours, weekends, on call then being written up for taking advantage of the overtime.  Oh well, tis life.  I wish this individual great success in weeding out the bad Apple's as myself and others that just can't take the pressure.  I hope she never succumbs to such an ordeal in her job.
   
  Signing off.  You all stay strong and hang in there.
   
  No since continuing this membership.

       
---------------------------------
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From CIngles <@t> uwhealth.org  Mon Mar 31 13:56:19 2008
From: CIngles <@t> uwhealth.org (Ingles Claire)
Date: Mon Mar 31 13:56:27 2008
Subject: [Histonet] Hematoxylin Shortage??
References: <006c01c89114$0b416e30$6401a8c0@DHXTS541><301368.35051.qm@web31307.mail.mud.yahoo.com>
	
Message-ID: <08A0A863637F1349BBFD83A96B27A50A12010C@uwhis-xchng3.uwhis.hosp.wisc.edu>

I don't know... We still make our heme from powder, mostly because no-one makes a commercial double strength Carazzis that we know of. But it isn't a big deal for us. Granted we use oxidizing agents so the solution is ready immediately (pending pH).But it doesn't seem to overoxidize so that staining is lost. I'm sure if we left it out, it would. But we use the fresh stuff up quicker than the formula oxidizes. We only really make up a liter bottle at a time, and only when the current bottle is almost empty. We filter in the morning before placing on the stainer. I agree with the assessment of the lack of practical for the certification exams. There are plenty of book smart people who can memorize, but are totally useless in day to day work as they don't know what the stains are really supposed to look like, and don't seem to be able to troubleshoot very well. I feel it's like getting one's driver' license without taking the road test (without as much immediate danger to life and limb).
Claire

________________________________

From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rittman, Barry R
Sent: Sun 3/30/2008 7:53 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Hematoxylin Shortage??



I also think that home made hematoxylin such as Ehrlich's is superior in it's staining.
I have heard some criticism of controlling the oxidation so that the hematoxylin does not overoxidise. We used to have several bottles and when one had reached its "peak" then transfer to a large separating funnel with a layer of paraffin oil on the surface. This prevented further oxidation and could just dispense amount needed from the stopcock at the base.
I was trained in the UK where cost was always a factor and labor used to be cheaper.
Here in the States it seems as if almost everyone relies on made up solutions. This has two problems. One is that individuals may (and I repeat may) not realize why there are certain components in solutions and  not as readily appreciate the basis of the staining and therefore able to correct problems when they arise.
I believe that the decision to eliminate the practical portion of the HT exam pushes us further in that direction.
When my granddaughter (who is almost 4) is able to prepare a meal for us on her own she will have received a lot of instruction in preparing dishes from scratch.
Barry




From CIngles <@t> uwhealth.org  Mon Mar 31 14:05:38 2008
From: CIngles <@t> uwhealth.org (Ingles Claire)
Date: Mon Mar 31 14:07:46 2008
Subject: [Histonet] Blackballed
References: <544090.42586.qm@web38202.mail.mud.yahoo.com>
Message-ID: <08A0A863637F1349BBFD83A96B27A50A12010D@uwhis-xchng3.uwhis.hosp.wisc.edu>

Steve - Would you still be available for temp work in the future? We'll take you any time we need extra help. Sorry to hear you go.
Claire Ingles
UW Mohs Clinic

________________________________

From: histonet-bounces@lists.utsouthwestern.edu on behalf of Steven Coakley
Sent: Mon 3/31/2008 1:50 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Blackballed



Well, I suppose I must accept that a certain Lab director has long ago blackballed me from working in Histology, at least, in my area.  Guess that's what one gets from rebelling against being so stressed out from excessive periods of short staffing and temps.  Working long hours, weekends, on call then being written up for taking advantage of the overtime.  Oh well, tis life.  I wish this individual great success in weeding out the bad Apple's as myself and others that just can't take the pressure.  I hope she never succumbs to such an ordeal in her job.
  
  Signing off.  You all stay strong and hang in there.
  
  No since continuing this membership.

      
---------------------------------
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From Mark.Frei <@t> sial.com  Mon Mar 31 14:37:23 2008
From: Mark.Frei <@t> sial.com (Mark Frei)
Date: Mon Mar 31 14:37:56 2008
Subject: [Histonet] Re:Hematoxylin Shortage
In-Reply-To: <200803301711.m2UHBhG1028817@stlspfw2.sial.com>
Message-ID: 

I didn't know the reason, but I can second Mark's statement on a shortage 
of hematoxylin.  The price per Kg of bulk has doubled...if you can get it 
at all!

Mark Frei   MT(ASCP)
Product Management
Sigma-Aldrich Corporation
3050 Spruce Street
St. Louis, MO  63103
(314) 286-8080

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otherwise have reason to believe that you have received this
message in error, please contact the sender and delete this message
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From carl.hobbs <@t> kcl.ac.uk  Mon Mar 31 14:45:32 2008
From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs)
Date: Mon Mar 31 14:45:59 2008
Subject: [Histonet] Hematoxylin Shortage??
Message-ID: <000901c89367$c8722ee0$4301a8c0@carlba65530bda>

Kemlo: I was attempting to be flippant: you know, we go to the Supermarket 
and buy "organic" produce.....
I do not deride such people as Wallington: they paved the way. Massive 
respect.
Well, I have more respect for Katie Page, who actually tried to make thing 
WORK, rather than regurgitating methodology...( even if she found her last 
lover in Eric......
according to the Papers I read..no way do I state this as my own )

Just that many got stuck in a rut.
We do.....
As I am approaching his age, when he dismissed me, I feel a similar 
"rear-guard" action.

 However, he had moved on by challenging those that came before him, also, 
surely?
Not sure but, Wallington served me very well, in the early days of my 
passion for histology.
Respect

( hur, hur, I still feel that way about another "bible" Theory and Practise 
of histological Techniques"...however, seems to me that the majority of 
Chapters are just regurgitations.....gimme Kiernan any time ;-)

Back tomounting media: Sure, each  mounting medium has it's 
place.....particularly regarding the all-important RI.
However, I have never noticed the difference, in routine Histological 
situations.
The navel-gazing regarding RI was based upon historical microscopic 
inadequacies...
Which are still evident today, sigh.....not many set up their microscopes 
( Kohler-wise) to avoid that GLARE.......sigh.
It is extremely important that new generations understand Kohler 
illumination, Firstly. Mounting medium, secondarily!
So many times, I see brightfield images that have that "glare"...

Yes, Barry: Sincere respects, but, "I feel canada balsam is better"......is 
it or not?
I think I understand your stance, as I come from the Canada balsam skool.
Luverly smell, too....;-)
However....open for discussion.
When I worked in dental stuff, we always used CB: when I became in charge, I 
swapped to DPX: good, good.
Dried quicker, gave a result quicker......who gave a damn about the minutiae 
of RI.
Anyway, Barry, ground sections do not comply.....they are too thick!
I know, I did them for several years: also , I did methyl methacrylate bone 
stuff, mounting in DPX....;-)
C


Best wishes,
Carlos.





From RSRICHMOND <@t> aol.com  Mon Mar 31 15:39:26 2008
From: RSRICHMOND <@t> aol.com (Robert Richmond)
Date: Mon Mar 31 15:39:56 2008
Subject: [Histonet] Re: DPX Canada balsam
In-Reply-To: 
References: 
Message-ID: 

Somebody asked >>I just drank an orange soda and was wondering what
"glycerol ester of
wood rosin" was.  What does it did to the soda?<<

I guess it makes the soda tastier if you happen to be a termite.

See
http://en.wikipedia.org/wiki/Glycerol_ester_of_wood_rosin
which will not encourage you to ingest the stuff.

Bob Richmond
Samurai Pathologist
Knoxville TN

From kdwyer3322 <@t> aol.com  Mon Mar 31 16:13:18 2008
From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com)
Date: Mon Mar 31 16:13:28 2008
Subject: [Histonet] Texas Society for Histotechnology 2008
	Symposium/Convention
Message-ID: <8CA618B2D6728A1-BF0-2004@WEBMAIL-MA17.sysops.aol.com>

To all: 

It's still not to late to sign up for the TSH meeting.? 
April 17-20, 2008
15 worshops and ?5 symposiums to choose from
38 vendors to visit

Activities Include: 

April 17, 2008 - Golf Tournament
April 18 - Presidents Reception/Exibits Open
April 19 - First Timers Breakfast, Memebership Lunch/Featured Speaker, and Awards Banquet with Music and Casino Night!!

If you would like more information or program?please contact me via this e-mail or go to our website at txsh.org? 

Rooms are still available at: 
DFW Hilton Lakes Executive Conference Center
1800 Highway 26 East 
Grapevine, Texas 76051
1-800-984-1344
$119.00 single/double

From AnthonyH <@t> chw.edu.au  Mon Mar 31 16:48:06 2008
From: AnthonyH <@t> chw.edu.au (Tony Henwood)
Date: Mon Mar 31 16:49:15 2008
Subject: [Histonet] Blackballed
In-Reply-To: <544090.42586.qm@web38202.mail.mud.yahoo.com>
Message-ID: 

That's why we have unions. Firstly to ensure that we are all treated as
humans!!

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven
Coakley
Sent: Tuesday, 1 April 2008 5:50 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Blackballed


Well, I suppose I must accept that a certain Lab director has long ago
blackballed me from working in Histology, at least, in my area.  Guess
that's what one gets from rebelling against being so stressed out from
excessive periods of short staffing and temps.  Working long hours,
weekends, on call then being written up for taking advantage of the
overtime.  Oh well, tis life.  I wish this individual great success in
weeding out the bad Apple's as myself and others that just can't take
the pressure.  I hope she never succumbs to such an ordeal in her job.
   
  Signing off.  You all stay strong and hang in there.
   
  No since continuing this membership.

       
---------------------------------
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one month at no cost. _______________________________________________
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From mcauliff <@t> umdnj.edu  Mon Mar 31 14:11:06 2008
From: mcauliff <@t> umdnj.edu (Geoff McAuliffe)
Date: Mon Mar 31 16:55:27 2008
Subject: [Histonet] embedding problem small zebrafish
In-Reply-To: <20080325095027.tzk9sa21ogg80so8@webmail.ugent.be>
References: <20080325095027.tzk9sa21ogg80so8@webmail.ugent.be>
Message-ID: <47F1374A.3020005@umdnj.edu>

Greetings Barbara:

    How are you fixing and processing the fish? We do plastic embedding 
of z-fish embryos and have no problems with morphology.

Geoff

Barbara Verstraeten wrote:
> Hello,
>
> I work on zebrafish from 44 hours to 6 days old (so they are very 
> small; max. 0.5 cm).
> I would like to perform immostainings on them (such as E-cadherin 
> staining). I've embedded my species in technovit 9100 but the cutting 
> of such small animals is very difficult.
> I did some stainings already and the antibody works but the problem is 
> that my tissue is of very bad quality!! When I have 50 sections only 1 
> is usefull.
> This is not very good!
>
> Can someone give me any suggestions on how to embed them so that they 
> are more easy to section and that I can still stain them with 
> different antibodies?
>
> Thank you all in advance!
>
> Kind regards,
>
> Barbara Verstraeten
> VMDB
> University of Ghent
> Belgium
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>


-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff@umdnj.edu
**********************************************



From llewllew <@t> shaw.ca  Mon Mar 31 17:39:26 2008
From: llewllew <@t> shaw.ca (Bryan Llewellyn)
Date: Mon Mar 31 17:39:41 2008
Subject: [Histonet] Hematoxylin Shortage??
References: <000901c89367$c8722ee0$4301a8c0@carlba65530bda>
Message-ID: <002501c89380$13531c90$92054246@yourlk4rlmsu>

I always thought that hematoxylin, coming as it does from a tree, was 
organic.

I went to Bromley Technical College on a Friday evening to be taught by 
Wallington, 1963 and 1964 possibly, the brain fails sometimes.  I ran afoul 
of him on one occasion, although I really didn't understand why at the time. 
He had told us the Schiff reagent is a leucobase thing and I had read 
somewhere else that it wasn't, so I asked in all innocence.  I wished I 
hadn't.  After not so oblique references to being careful that I didn't fail 
my exams (he was an examiner for the IMLT in histology Finals) I kept my 
mouth shut.  As it happens, he was an examiner for me at my practical and my 
oral and he passed me as well.

His great innovation was proving secondary fixation actually worked, as I 
recall.  I also recall he had no hesitation debunking the old mythological 
procedures.  Everyone has thei foibles, me included I'm sure.

Bryan Llewellyn

----- Original Message ----- 
From: "Carl Hobbs" 
To: "Histonet" 
Sent: Monday, March 31, 2008 12:45 PM
Subject: RE: [Histonet] Hematoxylin Shortage??


> Kemlo: I was attempting to be flippant: you know, we go to the Supermarket 
> and buy "organic" produce.....
> I do not deride such people as Wallington: they paved the way. Massive 
> respect.
> Well, I have more respect for Katie Page, who actually tried to make thing 
> WORK, rather than regurgitating methodology...( even if she found her last 
> lover in Eric......
> according to the Papers I read..no way do I state this as my own )
>
> Just that many got stuck in a rut.
> We do.....
> As I am approaching his age, when he dismissed me, I feel a similar 
> "rear-guard" action.
>
> However, he had moved on by challenging those that came before him, also, 
> surely?
> Not sure but, Wallington served me very well, in the early days of my 
> passion for histology.
> Respect
>
> ( hur, hur, I still feel that way about another "bible" Theory and 
> Practise of histological Techniques"...however, seems to me that the 
> majority of Chapters are just regurgitations.....gimme Kiernan any time 
> ;-)
>
> Back tomounting media: Sure, each  mounting medium has it's 
> place.....particularly regarding the all-important RI.
> However, I have never noticed the difference, in routine Histological 
> situations.
> The navel-gazing regarding RI was based upon historical microscopic 
> inadequacies...
> Which are still evident today, sigh.....not many set up their microscopes 
> ( Kohler-wise) to avoid that GLARE.......sigh.
> It is extremely important that new generations understand Kohler 
> illumination, Firstly. Mounting medium, secondarily!
> So many times, I see brightfield images that have that "glare"...
>
> Yes, Barry: Sincere respects, but, "I feel canada balsam is 
> better"......is it or not?
> I think I understand your stance, as I come from the Canada balsam skool.
> Luverly smell, too....;-)
> However....open for discussion.
> When I worked in dental stuff, we always used CB: when I became in charge, 
> I swapped to DPX: good, good.
> Dried quicker, gave a result quicker......who gave a damn about the 
> minutiae of RI.
> Anyway, Barry, ground sections do not comply.....they are too thick!
> I know, I did them for several years: also , I did methyl methacrylate 
> bone stuff, mounting in DPX....;-)
> C
>
>
> Best wishes,
> Carlos.
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


From dcojita <@t> tampabay.rr.com  Mon Mar 31 18:02:16 2008
From: dcojita <@t> tampabay.rr.com (dcojita@tampabay.rr.com)
Date: Mon Mar 31 18:08:31 2008
Subject: [Histonet] thin prep processor
Message-ID: 

Hi netters!  Does anyone know the price of a new (small throughput) thin
prep processor?  

From jnocito <@t> satx.rr.com  Mon Mar 31 18:48:09 2008
From: jnocito <@t> satx.rr.com (Joe Nocito)
Date: Mon Mar 31 18:48:14 2008
Subject: [Histonet] Hematoxylin Shortage.
References: <5C0BED61F529364E86309CADEA63FEF20163F3AE@TRFT-EX01.xRothGen.nhs.uk>
Message-ID: <004401c89389$ad015f10$0302a8c0@yourxhtr8hvc4p>

that's cold, just cold

----- Original Message ----- 
From: "Marshall Terry Dr,Consultant Histopathologist" 

To: "Robyn Vazquez" ; 
; 
Sent: Monday, March 31, 2008 9:00 AM
Subject: RE: [Histonet] Hematoxylin Shortage.


It works as well as his spell check:-)

Terry

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robyn
Vazquez
Sent: 31 March 2008 14:45
To: Histonet@lists.utsouthwestern.edu; sjchtascp@yahoo.com
Subject: Re: [Histonet] Hematoxylin Shortage.

Steven,
That really works?

Robyn

>>> "Steven Coakley"  3/30/2008 9:40 PM >>>

Concord grape juce and frozen blueberrys 50/50.  Blend on high for 2
minutes.  Strain, and bring to RT.  Stain progessively for 3-5 minted,
rince ibriefly in lukearm water then blue in scotts or a weak bluing
agent.  If that doesn't work chill and use over ice cream.  win win
solution.

  :-)


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