From CrochiereSteve <@t> aol.com Sun Jun 1 06:06:09 2008 From: CrochiereSteve <@t> aol.com (CrochiereSteve@aol.com) Date: Sun Jun 1 06:11:27 2008 Subject: [Histonet] HT opening Message-ID: Hi. I have an opening in my histology lab for a HT. We handle 20K+ cases per year. Excellent benefit package and very competitive pay scale. Responsibilities will include embedding, cutting, staining etc. Very automated lab. Computer skills a plus. Contact: Steven M. Crochiere, HT(ASCP) Histology Supervisor LifePath Partners @ Mercy Medical Center Springfield, MA 01104 413-748-9541 **************Get trade secrets for amazing burgers. Watch "Cooking with Tyler Florence" on AOL Food. (http://food.aol.com/tyler-florence?video=4&?NCID=aolfod00030000000002) From koellingr <@t> comcast.net Sun Jun 1 10:28:30 2008 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Sun Jun 1 10:28:39 2008 Subject: [Histonet] What is "IgG cut" Message-ID: <060120081528.27012.4842C01E0001C95D0000698422007623029D09020704040A0105@comcast.net> Sotiris, If by "antibody clearing" you are refering to antibody purification, then "Ig cut" is one of several methods to purify an ascites or culture supernatent. Try to purify antibodies from ascites with ammonium sulfate precipitation and it is referred to as an "ammonium sulfate cut". But it is dirty with not only antibodies but other proteins present. If you use a protein-A or protein-G column to do the purification, it can be referred to as an "Ig or immunoglobulin cut". Still not pure antibody but much better. Any lab can do these and any hybridoma lab or cell culture lab any and more of these techniques would be routine to do. A book on antibodies and antibody purification would tell you all the details but any good lab, just with minimal equipment on a counter, can do this. Ray Koelling PhenoPath Labs Seattle, WA -------------- Original message -------------- From: sotiris lakis > Hello to everyone. What is IgG cut? I only know it's about antibody clearing. > Can it be done in any laboratory? Could somebody give me a hint? > > Thanks > Sotiris Lakis > Resident in Pathology > Greece > > > --------------------------------- > Χρησιμοποιεί‘¦ Yahoo! > Βαρεθήκᑦ ‘¦ ενοχλ瑦κά μηνύ μᑦ (spam); Το Yahoo! Mail διαθέτει ‘¦ξΒκαλύ‘¦ρη > δυνᑦ προσ‘¦σίβΒκᑦ ‘¦ξΒενοχλ瑦κώξΒμηνυμά‘¦ξΒ > http://login.yahoo.com/config/mail?.intl=gr > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maxim_71 <@t> mail.ru Sun Jun 1 12:31:56 2008 From: Maxim_71 <@t> mail.ru (Maxim_71@mail.ru) Date: Sun Jun 1 12:36:56 2008 Subject: [Histonet] Embedding centers and Hematoxylin Message-ID: <253170855.20080601213156@mail.ru> 1- Our lab uses hot plate for embedding, which is bought in 1980 (manufactured in Russia). It still works OK. We have not embedding centre. 2- I itself prepare hematoxylin from Aldrich's powder by Lillie's recipe at each 2 weeks already many years with high quality staining. This is a progressive stain. Process of the preparation takes 20 minutes and we have always fresh hematoxyline. Sincerely, Maxim Peshkov, Russia, Taganrog. mailto:Maxim_71@mail.ru From akbitting <@t> geisinger.edu Mon Jun 2 06:23:52 2008 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Mon Jun 2 06:24:11 2008 Subject: [Histonet] Creative Waste Solutions Message-ID: <4843A008.2B7F.00C9.0@geisinger.edu> Would users of recycling products from this company share feedback with me? Thanks, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From relia1 <@t> earthlink.net Mon Jun 2 09:17:43 2008 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Jun 2 09:17:49 2008 Subject: [Histonet] RELIA Histology Careers Bulletin 06/02/08 Right Place Right Time Right Opportunity!! Message-ID: Hi Histonetters! Right Place, Right Time, Right Opportunity. Sometimes your next career move is just a matter of being in the right place at the right time and ready for the next opportunity. Odds are that if you are contemplating a job change for whatever reason ? better compensation, a more desirable location or more challenging work, you don?t have the time to do a job search. Let me help you with that. I have clients located throughout the country. All of the jobs that I represent are full time day shift positions unless otherwise noted, with excellent compensation, benefits and relocation/sign-on bonuses. If you are experienced or entry level, ASCP certified or eligible my clients are interested in speaking with you! I will assist you with your resume, coordination of interviews and coaching throughout the process. Remember my services are FREE of charge to you. Here is the latest update of the positions I am most excited to represent. If you are interested in any of these positions or a position like anyone of these but in another area either way ? give me a call toll free at 866-607-3542 or shoot me an e-mail at relia1@earthlink.net and let?s discuss it. Whether you are looking for a new position today, tomorrow or 6 months from now, it is never too early to have me keep a watch out for that perfect job for you! HISTOLOGY MANAGEMENT Central CA Los Angeles Irvine Dallas Phoenix HISTOTECHS FL ? Central and Southwest OH ? Cincinnati (night shift) TX ? San Antonio, Dallas and Austin WA ? Seattle area and Spokane, Clinical and Research positions NY - NYC MD ? Baltimore (grossing exper) and Western area VA ? Central VA CA- Los Angeles GA - Atlanta MA ? Cape Cod PATHOLOGY ASSISTANTS Western Maryland Tampa Bay, Florida Remember if nothing sounds interesting on my current list you can pass it on to your friends and wait for the next one OR give me a call or shoot me an e-mail telling me what you are looking for in your next opportunity. Remember timing is everything. Thanks ? Pam 866-60-RELIA (866-607-3542) Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.myspace.com/pamatrelia From Pat.Bell <@t> UCHSC.edu Mon Jun 2 11:22:11 2008 From: Pat.Bell <@t> UCHSC.edu (Pat.Bell@UCHSC.edu) Date: Mon Jun 2 11:22:19 2008 Subject: [Histonet] Laminin 5 Message-ID: <71FCC52823941D49A4BC39B48C6E3428F3C021@java.uchsc.edu> Hello to all, Can anyone recommend an antibody for human laminin 5? I had one from Chemicon that worked great, but now it doesn't work at all. Thank you so much. Pat Pat Bell HT(ASCP) Sr. PRA Division of Medical Oncology UCHSC, Aurora, CO 303-724-3845 (lab) 303-724-3889 (fax) From laurie.colbert <@t> huntingtonhospital.com Mon Jun 2 11:48:00 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Mon Jun 2 11:48:09 2008 Subject: [Histonet] Creative Waste Solutions Message-ID: <57BE698966D5C54EAE8612E8941D768303019DA3@EXCHANGE3.huntingtonhospital.com> Angie, We use their formalin recycler, and we love it. It is very easy to use. Laurie Colbert Huntington Hospital Pasadena, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Monday, June 02, 2008 4:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Creative Waste Solutions Would users of recycling products from this company share feedback with me? Thanks, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From Sandra.Harrison3 <@t> va.gov Mon Jun 2 12:19:57 2008 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Mon Jun 2 12:20:04 2008 Subject: [Histonet] dome lid coplin jars Message-ID: This is a small thing, but it would make my Pathology People so happy (and me too!!) if I could order some plastic coplin jars with the domed lid. Has anyone received any recently and if so, which supplier did you use and catalog number? I've tried Cardinal and Fisher with no luck. Also Electron Microscopy Sciences. They send the flat lidded, plastic coplin jar that is too deep. The problem with the flat lidded, plastic coplin jars we have received is that we can't pull slides out with just our gloved fingers, but must instead use forceps, because they are so deep. The catalogs show a picture of a domed lid, but they send you the flat lidded coplin jar. Any suggestions? Thanks, Sandy From Pat.Bell <@t> UCHSC.edu Mon Jun 2 12:23:01 2008 From: Pat.Bell <@t> UCHSC.edu (Pat.Bell@UCHSC.edu) Date: Mon Jun 2 12:23:05 2008 Subject: [Histonet] dome lid coplin jars In-Reply-To: Message-ID: <71FCC52823941D49A4BC39B48C6E3428F3C023@java.uchsc.edu> Try Market Lab and VWR. Pat Bell HT(ASCP) Sr. PRA Division of Medical Oncology UCHSC, Aurora, CO 303-724-3845 (lab) 303-724-3889 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C. Sent: Monday, June 02, 2008 11:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] dome lid coplin jars This is a small thing, but it would make my Pathology People so happy (and me too!!) if I could order some plastic coplin jars with the domed lid. Has anyone received any recently and if so, which supplier did you use and catalog number? I've tried Cardinal and Fisher with no luck. Also Electron Microscopy Sciences. They send the flat lidded, plastic coplin jar that is too deep. The problem with the flat lidded, plastic coplin jars we have received is that we can't pull slides out with just our gloved fingers, but must instead use forceps, because they are so deep. The catalogs show a picture of a domed lid, but they send you the flat lidded coplin jar. Any suggestions? Thanks, Sandy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kgrobert <@t> rci.rutgers.edu Mon Jun 2 12:41:13 2008 From: kgrobert <@t> rci.rutgers.edu (Kathleen Roberts) Date: Mon Jun 2 12:31:41 2008 Subject: [Histonet] dome lid coplin jars In-Reply-To: References: Message-ID: <484430B9.9000302@rci.rutgers.edu> How about MarketLab? www.marketlabinc.com Cat# ML9801 I'm about to order some myself, but before I do so, are these safe to use in the microwave? I think so, but I want to ask and be sure. -Kathy Roberts Principal Lab Technician Neurotoxicology Laboratories Dept of Pharmacology and Toxicology Ernest Mario School of Pharmacy Rutgers, the State University of NJ 41 B Gordon Rd Piscataway, NJ 08854 Harrison, Sandra C. wrote: >This is a small thing, but it would make my Pathology People so happy >(and me too!!) if I could order some plastic coplin jars with the domed >lid. Has anyone received any recently and if so, which supplier did you >use and catalog number? > >I've tried Cardinal and Fisher with no luck. Also Electron Microscopy >Sciences. They send the flat lidded, plastic coplin jar that is too >deep. > >The problem with the flat lidded, plastic coplin jars we have received >is that we can't pull slides out with just our gloved fingers, but must >instead use forceps, because they are so deep. The catalogs show a >picture of a domed lid, but they send you the flat lidded coplin jar. > >Any suggestions? > >Thanks, >Sandy >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histon > > From talulahgosh <@t> gmail.com Mon Jun 2 12:36:54 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Mon Jun 2 12:37:07 2008 Subject: [Histonet] bausch surgical Message-ID: Hello We need to sharpen our Vannas scissors, which we usually do through Storz. Unfortunately, we haven't done this for about four years, and Storz is now part of Bausch Surgical. Their website doesn't have any information on where to send them and I've been on hold for ten minutes twice without reaching anyone. Does anyone here have their recent address or a non-toll free number for them? Thanks, Emily -- When you're riding in a time machine way far into the future, don't stick your elbow out the window, or it'll turn into a fossil. From MAUGER <@t> email.chop.edu Mon Jun 2 14:41:59 2008 From: MAUGER <@t> email.chop.edu (Joanne Mauger) Date: Mon Jun 2 14:42:36 2008 Subject: [Histonet] enzyme histochemistry- automation Message-ID: Hi Everyone, Does anyone know of an automated platform for staining fresh(frozen) muscle slides for ATP, NADH, COX, etc.? I know it's a long shot, but I had to ask. Thanks, Jo From Erin.Martin <@t> ucsf.edu Mon Jun 2 15:28:15 2008 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Mon Jun 2 15:28:26 2008 Subject: [Histonet] Toluidine Blue and H&E Message-ID: Hi all, My pathologist would like to do a combined toluidine blue and H&E. Has anyone ever done this? If so, would you mind sharing your procedure? I am guessing that I would have to use the toluidine blue after the eosin then air dry the slides since alcohol would take the blue out, but I am not sure if it would react with either the hematoxylin or the eosin. Thank you for your help! Erin Martin From rjbuesa <@t> yahoo.com Mon Jun 2 15:43:45 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jun 2 15:43:48 2008 Subject: [Histonet] Toluidine Blue and H&E In-Reply-To: Message-ID: <372902.76788.qm@web65712.mail.ac4.yahoo.com> I would try it first without the hematoxylin and before the eosin. You don't have to air dry the sections, just blot them and place them in isopropanol before going to xylene. Toluidin blue works better at pH 11 that you can reach with borax before dissolving the dye in it. It works also better at 50-60?C. Hope this will help you. Ren? J. "Martin, Erin" wrote: Hi all, My pathologist would like to do a combined toluidine blue and H&E. Has anyone ever done this? If so, would you mind sharing your procedure? I am guessing that I would have to use the toluidine blue after the eosin then air dry the slides since alcohol would take the blue out, but I am not sure if it would react with either the hematoxylin or the eosin. Thank you for your help! Erin Martin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Mon Jun 2 15:46:52 2008 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Jun 2 15:46:54 2008 Subject: [Histonet] Trying to reach Tracey Lenek Message-ID: Hi Tracey, I tried to respond to your e-mail but the address that I am replying to is bouncing back as undeliverable. The answer to your question is yes and when you get a chance please send me a new e-mail address or phone number to reach you. Thanks-Pam Thank You! Pam M. Barker President Relia 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll Free: (866)607-3542 e-mail: relia1@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia From myl__28 <@t> hotmail.com Tue Jun 3 07:56:20 2008 From: myl__28 <@t> hotmail.com (=?iso-8859-1?Q?Myl=E8ne_de_Champlain?=) Date: Tue Jun 3 07:56:27 2008 Subject: [Histonet] Histological products Message-ID: Hi, I would like to know if those histological products still good even if they are expired. They still sealed in the original package. Products Expiration Consul-mount histology formulation 09-2007 Shandon xylene-substitute mountant 01-2007 Instant hematoxylin (powder) 09-2003 Thank you for your answers. Myl?ne Universit? du Qu?bec ? Rimouski _________________________________________________________________ From rjbuesa <@t> yahoo.com Tue Jun 3 08:14:56 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jun 3 08:15:01 2008 Subject: [Histonet] Histological products In-Reply-To: Message-ID: <36234.55354.qm@web65716.mail.ac4.yahoo.com> If sealed in their original package (not exposed to air), they probably are. On the other hand, it will be always cheaper to prepare a hematoxylin solution to determine how it works, than to buy a new powder bottle. Ren? J. Myl?ne de Champlain wrote: Hi, I would like to know if those histological products still good even if they are expired. They still sealed in the original package. Products Expiration Consul-mount histology formulation 09-2007 Shandon xylene-substitute mountant 01-2007 Instant hematoxylin (powder) 09-2003 Thank you for your answers. Myl?ne Universit? du Qu?bec ? Rimouski _________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jerry <@t> ralambusa.com Tue Jun 3 12:11:46 2008 From: Jerry <@t> ralambusa.com (Jerry Helisek) Date: Tue Jun 3 12:11:54 2008 Subject: [Histonet] RE: Histonet Digest, Vol 55, Issue 3 Message-ID: <3855F92002259948A66A8CA2D16E3A4F0B544E@server.ralambusa.com> Sandy: This may work for you... See: http://www.ralamb.net/product_info.php?products_id=347 Thanks ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ Message: 1 Date: Mon, 2 Jun 2008 12:19:57 -0500 From: "Harrison, Sandra C." Subject: [Histonet] dome lid coplin jars To: Message-ID: Content-Type: text/plain; charset="us-ascii" This is a small thing, but it would make my Pathology People so happy (and me too!!) if I could order some plastic coplin jars with the domed lid. Has anyone received any recently and if so, which supplier did you use and catalog number? I've tried Cardinal and Fisher with no luck. Also Electron Microscopy Sciences. They send the flat lidded, plastic coplin jar that is too deep. The problem with the flat lidded, plastic coplin jars we have received is that we can't pull slides out with just our gloved fingers, but must instead use forceps, because they are so deep. The catalogs show a picture of a domed lid, but they send you the flat lidded coplin jar. Any suggestions? Thanks, Sandy From RSRICHMOND <@t> aol.com Tue Jun 3 12:20:50 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Tue Jun 3 12:20:57 2008 Subject: [Histonet] Re: Toluidine Blue and H&E Message-ID: Erin Martin asks: >>My pathologist would like to do a combined toluidine blue and H&E. Has anyone ever done this? If so, would you mind sharing your procedure? I am guessing that I would have to use the toluidine blue after the eosin then air dry the slides since alcohol would take the blue out, but I am not sure if it would react with either the hematoxylin or the eosin.<< What an interesting idea! I suppose we're talking about staining Helicobacter in gastric biopsy specimens. I was one of the early users of toluidine blue for Helicobacter, and I've more or less followed the literature but have never found such a technique. As others have suggested, I suppose the toluidine blue would come after the H & E. Moving the slides rapidly through alcohols would probably keep the dye from washing out. Most people would probably use Diff-Quik II (or a generic equivalent) rather than preparing toluidine blue. The only way to find out is to try it! I'd want you to have good control sections to practice with, and I'd expect to work closely with the histotechnologist to get the desired result. I hope somebody does try this and report back to us all. Bob Richmond Samurai Pathologist Knoxville TN From DEllenburg2 <@t> stfrancishealth.org Tue Jun 3 12:44:47 2008 From: DEllenburg2 <@t> stfrancishealth.org (Ellenburg, Deborah) Date: Tue Jun 3 12:44:59 2008 Subject: [Histonet] Plastic coplin Jar with Domed Lid In-Reply-To: References: Message-ID: Sandy, I went online and searched for domed lid coplin jars and came up with the following. Thermo Scientific - Shandon Plastic Coplin Staining Jar - SKU: 194 Hope this helps. Debbie E. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, June 03, 2008 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 55, Issue 3 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. dome lid coplin jars (Harrison, Sandra C.) 2. RE: dome lid coplin jars (Pat.Bell@UCHSC.edu) 3. Re: dome lid coplin jars (Kathleen Roberts) 4. bausch surgical (Emily Sours) 5. enzyme histochemistry- automation (Joanne Mauger) 6. Toluidine Blue and H&E (Martin, Erin) 7. Re: Toluidine Blue and H&E (Rene J Buesa) 8. Trying to reach Tracey Lenek (Pam Barker) 9. Histological products (Myl?ne de Champlain) 10. Re: Histological products (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Mon, 2 Jun 2008 12:19:57 -0500 From: "Harrison, Sandra C." Subject: [Histonet] dome lid coplin jars To: Message-ID: Content-Type: text/plain; charset="us-ascii" This is a small thing, but it would make my Pathology People so happy (and me too!!) if I could order some plastic coplin jars with the domed lid. Has anyone received any recently and if so, which supplier did you use and catalog number? I've tried Cardinal and Fisher with no luck. Also Electron Microscopy Sciences. They send the flat lidded, plastic coplin jar that is too deep. The problem with the flat lidded, plastic coplin jars we have received is that we can't pull slides out with just our gloved fingers, but must instead use forceps, because they are so deep. The catalogs show a picture of a domed lid, but they send you the flat lidded coplin jar. Any suggestions? Thanks, Sandy ------------------------------ Message: 2 Date: Mon, 2 Jun 2008 11:23:01 -0600 From: Subject: RE: [Histonet] dome lid coplin jars To: , Message-ID: <71FCC52823941D49A4BC39B48C6E3428F3C023@java.uchsc.edu> Content-Type: text/plain; charset="US-ASCII" Try Market Lab and VWR. Pat Bell HT(ASCP) Sr. PRA Division of Medical Oncology UCHSC, Aurora, CO 303-724-3845 (lab) 303-724-3889 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C. Sent: Monday, June 02, 2008 11:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] dome lid coplin jars This is a small thing, but it would make my Pathology People so happy (and me too!!) if I could order some plastic coplin jars with the domed lid. Has anyone received any recently and if so, which supplier did you use and catalog number? I've tried Cardinal and Fisher with no luck. Also Electron Microscopy Sciences. They send the flat lidded, plastic coplin jar that is too deep. The problem with the flat lidded, plastic coplin jars we have received is that we can't pull slides out with just our gloved fingers, but must instead use forceps, because they are so deep. The catalogs show a picture of a domed lid, but they send you the flat lidded coplin jar. Any suggestions? Thanks, Sandy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Mon, 02 Jun 2008 13:41:13 -0400 From: Kathleen Roberts Subject: Re: [Histonet] dome lid coplin jars To: "Harrison, Sandra C." Cc: "'histonet@lists.utsouthwestern.edu'" Message-ID: <484430B9.9000302@rci.rutgers.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed How about MarketLab? www.marketlabinc.com Cat# ML9801 I'm about to order some myself, but before I do so, are these safe to use in the microwave? I think so, but I want to ask and be sure. -Kathy Roberts Principal Lab Technician Neurotoxicology Laboratories Dept of Pharmacology and Toxicology Ernest Mario School of Pharmacy Rutgers, the State University of NJ 41 B Gordon Rd Piscataway, NJ 08854 Harrison, Sandra C. wrote: >This is a small thing, but it would make my Pathology People so happy >(and me too!!) if I could order some plastic coplin jars with the domed >lid. Has anyone received any recently and if so, which supplier did you >use and catalog number? > >I've tried Cardinal and Fisher with no luck. Also Electron Microscopy >Sciences. They send the flat lidded, plastic coplin jar that is too >deep. > >The problem with the flat lidded, plastic coplin jars we have received >is that we can't pull slides out with just our gloved fingers, but must >instead use forceps, because they are so deep. The catalogs show a >picture of a domed lid, but they send you the flat lidded coplin jar. > >Any suggestions? > >Thanks, >Sandy >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histon > > ------------------------------ Message: 4 Date: Mon, 2 Jun 2008 13:36:54 -0400 From: "Emily Sours" Subject: [Histonet] bausch surgical To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hello We need to sharpen our Vannas scissors, which we usually do through Storz. Unfortunately, we haven't done this for about four years, and Storz is now part of Bausch Surgical. Their website doesn't have any information on where to send them and I've been on hold for ten minutes twice without reaching anyone. Does anyone here have their recent address or a non-toll free number for them? Thanks, Emily -- When you're riding in a time machine way far into the future, don't stick your elbow out the window, or it'll turn into a fossil. ------------------------------ Message: 5 Date: Mon, 02 Jun 2008 15:41:59 -0400 From: "Joanne Mauger" Subject: [Histonet] enzyme histochemistry- automation To: , Message-ID: Content-Type: text/plain; charset=US-ASCII Hi Everyone, Does anyone know of an automated platform for staining fresh(frozen) muscle slides for ATP, NADH, COX, etc.? I know it's a long shot, but I had to ask. Thanks, Jo ------------------------------ Message: 6 Date: Mon, 2 Jun 2008 13:28:15 -0700 From: "Martin, Erin" Subject: [Histonet] Toluidine Blue and H&E To: "histonet" Message-ID: Content-Type: text/plain; charset=iso-8859-1 Hi all, My pathologist would like to do a combined toluidine blue and H&E. Has anyone ever done this? If so, would you mind sharing your procedure? I am guessing that I would have to use the toluidine blue after the eosin then air dry the slides since alcohol would take the blue out, but I am not sure if it would react with either the hematoxylin or the eosin. Thank you for your help! Erin Martin ------------------------------ Message: 7 Date: Mon, 2 Jun 2008 13:43:45 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Toluidine Blue and H&E To: "Martin, Erin" , histonet Message-ID: <372902.76788.qm@web65712.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I would try it first without the hematoxylin and before the eosin. You don't have to air dry the sections, just blot them and place them in isopropanol before going to xylene. Toluidin blue works better at pH 11 that you can reach with borax before dissolving the dye in it. It works also better at 50-60?C. Hope this will help you. Ren? J. "Martin, Erin" wrote: Hi all, My pathologist would like to do a combined toluidine blue and H&E. Has anyone ever done this? If so, would you mind sharing your procedure? I am guessing that I would have to use the toluidine blue after the eosin then air dry the slides since alcohol would take the blue out, but I am not sure if it would react with either the hematoxylin or the eosin. Thank you for your help! Erin Martin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Mon, 2 Jun 2008 16:46:52 -0400 From: "Pam Barker" Subject: [Histonet] Trying to reach Tracey Lenek To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Tracey, I tried to respond to your e-mail but the address that I am replying to is bouncing back as undeliverable. The answer to your question is yes and when you get a chance please send me a new e-mail address or phone number to reach you. Thanks-Pam Thank You! Pam M. Barker President Relia 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll Free: (866)607-3542 e-mail: relia1@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia ------------------------------ Message: 9 Date: Tue, 3 Jun 2008 08:56:20 -0400 From: Myl?ne de Champlain Subject: [Histonet] Histological products To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi, I would like to know if those histological products still good even if they are expired. They still sealed in the original package. Products Expiration Consul-mount histology formulation 09-2007 Shandon xylene-substitute mountant 01-2007 Instant hematoxylin (powder) 09-2003 Thank you for your answers. Myl?ne Universit? du Qu?bec ? Rimouski _________________________________________________________________ ------------------------------ Message: 10 Date: Tue, 3 Jun 2008 06:14:56 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Histological products To: "Myl?ne" de Champlain , histonet@lists.utsouthwestern.edu Message-ID: <36234.55354.qm@web65716.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 If sealed in their original package (not exposed to air), they probably are. On the other hand, it will be always cheaper to prepare a hematoxylin solution to determine how it works, than to buy a new powder bottle. Ren? J. Myl?ne de Champlain wrote: Hi, I would like to know if those histological products still good even if they are expired. They still sealed in the original package. Products Expiration Consul-mount histology formulation 09-2007 Shandon xylene-substitute mountant 01-2007 Instant hematoxylin (powder) 09-2003 Thank you for your answers. Myl?ne Universit? du Qu?bec ? Rimouski _________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 55, Issue 3 *************************************** ________________________________________________________________________________________________________________________________ ________________________________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. From Melissa.Gonzalez <@t> cellgenesys.com Tue Jun 3 13:31:25 2008 From: Melissa.Gonzalez <@t> cellgenesys.com (Melissa Gonzalez) Date: Tue Jun 3 13:31:37 2008 Subject: [Histonet] Alternative to Histobath Message-ID: <2884B897182A1D438C7BA24B9A8F94A2348005@hqsvr01mail.cgi.com> Anyone have any recommendations on tissue freezing units? Thanks! Melissa Melissa A. Gonz?lez Edick R&D, Cell Genesys, Inc 500 Forbes Blvd South San Francisco, CA 94080 ph (650) 266-3168 fx (650) 266-3080 www.cellgenesys.com From Stephen.Clark1 <@t> hcahealthcare.com Tue Jun 3 13:31:39 2008 From: Stephen.Clark1 <@t> hcahealthcare.com (Clark Stephen - Myrtle Beach) Date: Tue Jun 3 13:31:45 2008 Subject: [Histonet] Correlation studies Message-ID: <28E40C736ED32341BBB2B094EFD23FC8020F070B@NASEV05.hca.corpad.net> I need to perform correlation studies for our new Immunohistochemistry machine. The question I have is what format do I use? How do I do it? What should the test look like? Etc. Any advice would be appreciated. Thanks, Steve Clark From freckles9660 <@t> yahoo.com Tue Jun 3 14:22:36 2008 From: freckles9660 <@t> yahoo.com (Karla Arrington) Date: Tue Jun 3 14:22:41 2008 Subject: [Histonet] traveling Message-ID: <951179.57551.qm@web32501.mail.mud.yahoo.com> Fellow Histo's: I was wondering if anyone has done the "traveling Histotech" thing? Is it worth it? Need a change of pace... Very interested! Thanks! Karla Arrington, HT(ASCP) freckles9660@yahoo.com From tjohnson <@t> bostwicklaboratories.com Tue Jun 3 14:23:53 2008 From: tjohnson <@t> bostwicklaboratories.com (Tawanda Johnson) Date: Tue Jun 3 14:23:57 2008 Subject: [Histonet] Employment Opportunities Message-ID: <24D22DE9E488AA43BF92A4389F2DDB1F02BB2C07@mail1.BOSTWICK.COM> Bostwick Laboratories are currently seeking experienced Histotech's for our Ohio and Connecticut locations. If you have at least 2years laboratory experience and a Bachelor in Science Degree please email me your Resume. ASCP certification preferred. From rjbuesa <@t> yahoo.com Tue Jun 3 14:59:37 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jun 3 14:59:43 2008 Subject: [Histonet] Correlation studies In-Reply-To: <28E40C736ED32341BBB2B094EFD23FC8020F070B@NASEV05.hca.corpad.net> Message-ID: <408291.80171.qm@web65716.mail.ac4.yahoo.com> Correlations are always between two sets of variables (unless they refer to multiple correlations where those sets contain more than two). I have never heard of correlating an IHC machine. What are you going to correlate it with? I do not imagine how you are going to present the problem. Are you trying to correlate your results with your IHC machine with the results in another or with some other results? If that is the case you will have to run both sets simultaneously. On the other hand those correlation tests (the strongest) have to have a normal distribution of the data, or you will have to use some type of transformation. Perhaps you could find more information in http://www.vassar.edu/lowry which is a fantastic web site to perform statistical tests. Ren? J. Clark Stephen - Myrtle Beach wrote: I need to perform correlation studies for our new Immunohistochemistry machine. The question I have is what format do I use? How do I do it? What should the test look like? Etc. Any advice would be appreciated. Thanks, Steve Clark _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leiker <@t> buffalo.edu Tue Jun 3 15:59:52 2008 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Tue Jun 3 15:59:58 2008 Subject: [Histonet] xylene grades In-Reply-To: <408291.80171.qm@web65716.mail.ac4.yahoo.com> References: <408291.80171.qm@web65716.mail.ac4.yahoo.com> Message-ID: <8E75CCAA47872DF1593BF16B@bchwxp2702.ad.med.buffalo.edu> Would the grade of xylenes used for deparaffinizing make a difference for immunostaining of certain (picky) epitopes? We are using Certified A.C.S. xylenes. What about rinsing in tap water vs. distilled water during rehydration? The reason I'm asking is that we are having issues staining for c-kit (KIT, CD117) on FFPE tissue. We've tried all different antigen retrievals, including no retrieval, and still aren't seeing any stain. Any help would be greatly appreciated. Thanks! Merced M Leiker Research Technician II 354 BRB (Lee Lab) / 140 Farber Hall (mail) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 From jnocito <@t> satx.rr.com Tue Jun 3 18:55:08 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Jun 3 18:54:26 2008 Subject: [Histonet] Correlation studies References: <28E40C736ED32341BBB2B094EFD23FC8020F070B@NASEV05.hca.corpad.net> Message-ID: <003e01c8c5d5$4167d990$0302a8c0@yourxhtr8hvc4p> Steve, if you have been performing immunos manually, you can test the machine results against the manual ones. Run slides manually and on the machine. Reagents should be the same lot number. When I switched to an automated machine, I had to test each antibody and each HIER method. I kept the records and slides for 2 CAP cycles, a bit anal, but I wanted to be sure that CAP understood that I did have a validation system. Good luck Joe ----- Original Message ----- From: "Clark Stephen - Myrtle Beach" To: Sent: Tuesday, June 03, 2008 1:31 PM Subject: [Histonet] Correlation studies I need to perform correlation studies for our new Immunohistochemistry machine. The question I have is what format do I use? How do I do it? What should the test look like? Etc. Any advice would be appreciated. Thanks, Steve Clark _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MichelM9 <@t> chw.edu.au Tue Jun 3 23:20:26 2008 From: MichelM9 <@t> chw.edu.au (Michelle McDonald) Date: Tue Jun 3 23:20:40 2008 Subject: [Histonet] MMA de-plasticizing In-Reply-To: <62A8156F8071C8439080D626DF8C33A602E40C@wave-mail.7thwave.local> Message-ID: <47BD6E7614A693499453835D47E36F70045F0AD8@hedwig.nch.kids> Dear Michelle, We de-plasticise MMP bone sections using two changes of 100% acetone for 10 monis each. Thanks Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele Wich Sent: Saturday, 31 May 2008 1:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MMA de-plasticizing Is there anyone out there doing MMA on un-decalcified bone? I'm wondering if there is any way to de-plasticize without putting the slides in heated xylene--a potentially explosive situation which technically would require explosion proof oven, hood, clothing, etc. I know that the flash point of xylene is quite low (26.1?C, I think, which is barely above room temperature). Is there a way around this safety issue? This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From ales.kladnik <@t> bf.uni-lj.si Wed Jun 4 02:52:54 2008 From: ales.kladnik <@t> bf.uni-lj.si (=?UTF-8?B?QWxlxaEgS2xhZG5paw==?=) Date: Wed Jun 4 02:53:07 2008 Subject: [Histonet] Re: dome lid coplin jars Message-ID: <484649D6.6080807@bf.uni-lj.si> Try Bel-Art, they used to sell dome shaped coplin jars, now they claim they have an improved design with large opening - they even show a gloved hand taking a slide in/out of the jar. http://www.belart.com/cat/lifescience/en/442081000.asp > This is a small thing, but it would make my Pathology People so happy > (and me too!!) if I could order some plastic coplin jars with the domed > lid. Has anyone received any recently and if so, which supplier did you > use and catalog number? > > I've tried Cardinal and Fisher with no luck. Also Electron Microscopy > Sciences. They send the flat lidded, plastic coplin jar that is too > deep. > > The problem with the flat lidded, plastic coplin jars we have received > is that we can't pull slides out with just our gloved fingers, but must > instead use forceps, because they are so deep. The catalogs show a > picture of a domed lid, but they send you the flat lidded coplin jar. > > Any suggestions? > > Thanks, > Sandy -- Ale? Kladnik Univerza v Ljubljani, Biotehni?ka fakulteta, Oddelek za biologijo Ve?na pot 111, SI-1000 Ljubljana, Slovenija tel: +386 1 4233388, fax: +386 1 2573390 url: http://botanika.biologija.org/kdosmo/aleskl.php url: http://web.bf.uni-lj.si/bi/mikroskopija/ From j.h.reilly <@t> clinmed.gla.ac.uk Wed Jun 4 03:46:12 2008 From: j.h.reilly <@t> clinmed.gla.ac.uk (Jim Reilly) Date: Wed Jun 4 03:46:19 2008 Subject: [Histonet] Re: Toluidine Blue and H&E Message-ID: <82B54C1E04D94D489448735E6CBDE4606DC2B5@exchange-be5.centre.ad.gla.ac.uk> Hello I Tried Toluidine Blue / Eosin on decalcified mouse footpads : 2 minutes Tol Blue 1 minute Eosin rapid dehydration in 95% alcohol x1 100% x2 (10 dips each) clear in xylene and mount in DPX. This seemed to work OK. Jim Reilly Glasgow Biomedical Research Centre From abright <@t> brightinstruments.com Wed Jun 4 05:39:23 2008 From: abright <@t> brightinstruments.com (Alan Bright) Date: Wed Jun 4 06:07:57 2008 Subject: [Histonet] Alternative to Histobath References: <2884B897182A1D438C7BA24B9A8F94A2348005@hqsvr01mail.cgi.com> Message-ID: Dear Melissa, Yes, we manufacture the Clini-RF Rapid Freezer which goes down to -80 degs. (See our Web site) C. We also fit as an option a Quick Freezer into our OTF5000 Cryostat. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype: dazzle0 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Melissa Gonzalez Sent: 03 June 2008 19:31 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Alternative to Histobath Anyone have any recommendations on tissue freezing units? Thanks! Melissa Melissa A. Gonz?lez Edick R&D, Cell Genesys, Inc 500 Forbes Blvd South San Francisco, CA 94080 ph (650) 266-3168 fx (650) 266-3080 www.cellgenesys.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Wed Jun 4 06:33:23 2008 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Wed Jun 4 06:33:31 2008 Subject: [Histonet] GMA immunos In-Reply-To: References: <2884B897182A1D438C7BA24B9A8F94A2348005@hqsvr01mail.cgi.com> Message-ID: <7722595275A4DD4FA225B92CDBF174A172A577C87E@EXC-MBX3.cfs.le.ac.uk> After a lifetime of immunostaining paraffin wax sections, in my new job I am having to deal with GMA sections. Two questions, firstly, does one have to float out the sections for a minimum of 90 seconds on 0.5% ammonium hydroxide before mounting on a slide;( with the ammonaical water apparently acting as some sort of antigen retrieval step), and immunohistochemistry on GMA sections does not work if one uses PBS as opposed to TBS. Any help/hints gratefully received. Cheers Richard Edwards University of Leicester. U.K. From anitathorn <@t> comcast.net Wed Jun 4 07:06:16 2008 From: anitathorn <@t> comcast.net (anitathorn@comcast.net) Date: Wed Jun 4 07:06:24 2008 Subject: [Histonet] Creative Waste Solutions Message-ID: <060420081206.14063.4846853800058ACF000036EF2200750330029D01089B0E9B07020E@comcast.net> I work in a small volume lab and find that both the formalin and alcohol recycling systems are perfect for our needs. Easy to use and very economical. -------------- Original message ---------------------- From: "Laurie Colbert" > Angie, > > We use their formalin recycler, and we love it. It is very easy to use. > > Laurie Colbert > Huntington Hospital > Pasadena, CA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela > Bitting > Sent: Monday, June 02, 2008 4:24 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Creative Waste Solutions > > Would users of recycling products from this company share feedback with > me? > > Thanks, > Angie > > Angela Bitting, HT(ASCP) > Technical Specialist, Histology > Geisinger Medical Center > 100 N Academy Ave. MC 23-00 > Danville, PA 17822 > phone 570-214-9634 > fax 570-271-5916 > > No trees were hurt in the sending of this email > However many electrons were severly inconvienienced! > > > > > IMPORTANT WARNING: The information in this message (and the documents > attached to it, if any) is confidential and may be legally privileged. > It is intended solely for the addressee. Access to this message by > anyone else is unauthorized. If you are not the intended recipient, any > disclosure, copying, distribution or any action taken, or omitted to be > taken, in reliance on it is prohibited and may be unlawful. If you have > received this message in error, please delete all electronic copies of > this message (and the documents attached to it, if any), destroy any > hard copies you may have created and notify me immediately by replying > to this email. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Wed Jun 4 07:09:56 2008 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Jun 4 07:10:04 2008 Subject: [Histonet] MLT working histology? In-Reply-To: <483D9B7F.603@sch-farmville.org> Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F57A@lmhsmail.lmhealth.org> I've had 3 or 4 MLT's over the years. They were trained to do every thing the HT's do. They were hired with the stipulation that they got their HT certification as well as having their MLT certification but only because it was a requirement for all of us. They have worked out well. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jennie Jenkins Sent: Wednesday, May 28, 2008 1:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MLT working histology? Can an MLT work in histology? She would be embedding, cutting, staining, cover slipping, labeling, filing, requisitioning and setting up the gross. If possible we could have her trained to gross in anything that would not require "cutting", like GI biopsies. Jennie Jenkins, PA(ASCP) Southside Community Hospital 800 Oak Street Farmville, Virginia 23901 ================================================== This e-mail message (and attachments) may contain information that is confidential to Southside Community Hospital. If you are not the intended recipient you cannot use, distribute or copy the message or attachments. In such a case, please notify the sender by return e-mail immediately and erase all copies of the message and attachments. Opinions, conclusions and other information in this message and attachments that do not relate to the official business of Southside Community Hospital are neither given nor endorsed by it. ================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Jun 4 07:12:24 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 4 07:12:26 2008 Subject: [Histonet] xylene grades In-Reply-To: <8E75CCAA47872DF1593BF16B@bchwxp2702.ad.med.buffalo.edu> Message-ID: <472664.60493.qm@web65713.mail.ac4.yahoo.com> The xylene "grade" (if such a thing exists!) is of a lesser concern than assuring a complete dewaxing of the section. You have to completely eliminate all the paraffin. Using distilled water to later immerse the sections in a buffer (that contains salts) is of no relevance, because you are going to "salinize" the sections any way. I think that your problem is with your Ab supplier, or the lot. CD117 is a "tricky" one. I stopped having problems when I switched to DAKO Mo Ab and HIER at pH6 Ren? J. Merced Leiker wrote: Would the grade of xylenes used for deparaffinizing make a difference for immunostaining of certain (picky) epitopes? We are using Certified A.C.S. xylenes. What about rinsing in tap water vs. distilled water during rehydration? The reason I'm asking is that we are having issues staining for c-kit (KIT, CD117) on FFPE tissue. We've tried all different antigen retrievals, including no retrieval, and still aren't seeing any stain. Any help would be greatly appreciated. Thanks! Merced M Leiker Research Technician II 354 BRB (Lee Lab) / 140 Farber Hall (mail) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Wed Jun 4 07:20:19 2008 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Wed Jun 4 07:20:55 2008 Subject: [Histonet] MLT working histology? In-Reply-To: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F57A@lmhsmail.lmhealth.org> Message-ID: <979FF5962E234F45B06CF0DB7C1AABB20FEC203C@chi2k3ms01.columbuschildrens.net> Why couldn't she? I have an MLT (taking her certification this year) and she is far superior to many of the "old timers" who claim to be histotechs but have absolutely no theoretical knowledge and are thus hopeless when it comes to troubleshooting. This profession needs to take the bull by the horns and change the outlook of histotechnology - it is a biomedical scientific profession and the training and examinations should reflect this. It is already lagging far behind MT's. The HT examination is a joke, and the QIHC certification is not much better. Now let the flaming begin!!!!!!!!!!! Ronnie Houston, MS, HT(ASCP)QIHC, AIBMS Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 Ronald.Houston@NationwideChildrens.org www.NationwideChildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Wednesday, June 04, 2008 8:10 AM To: jjenkins@sch-farmville.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] MLT working histology? I've had 3 or 4 MLT's over the years. They were trained to do every thing the HT's do. They were hired with the stipulation that they got their HT certification as well as having their MLT certification but only because it was a requirement for all of us. They have worked out well. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jennie Jenkins Sent: Wednesday, May 28, 2008 1:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MLT working histology? Can an MLT work in histology? She would be embedding, cutting, staining, cover slipping, labeling, filing, requisitioning and setting up the gross. If possible we could have her trained to gross in anything that would not require "cutting", like GI biopsies. Jennie Jenkins, PA(ASCP) Southside Community Hospital 800 Oak Street Farmville, Virginia 23901 ================================================== This e-mail message (and attachments) may contain information that is confidential to Southside Community Hospital. If you are not the intended recipient you cannot use, distribute or copy the message or attachments. In such a case, please notify the sender by return e-mail immediately and erase all copies of the message and attachments. Opinions, conclusions and other information in this message and attachments that do not relate to the official business of Southside Community Hospital are neither given nor endorsed by it. ================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From relia1 <@t> earthlink.net Wed Jun 4 07:48:41 2008 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Jun 4 07:48:51 2008 Subject: [Histonet] RELIA Special JOB Alert Message-ID: Hi Histonetters! I had one more position come in that I am pretty excited about. I have a client in the Philadelphia area in need of a senior histotechnologist. They are looking for someone who is ASCP certified with at least 5 years of experience. My client offers excellent compensation, benefits and a great place to work. Dermatopathology experience is a plus. If you are interested please contact me. I can be reached at relia1@earthlink.net or toll free at 866-607-3542. If you know someone who might be interested please feel free to pass the information along to them as well. Incidentally if you happen to know an ASCP certified med tech with microbiology experience I have a full time permanent 1st shift position in Cincinnati. Thanks and have a great day!! - Pam Thank You! Pam M. Barker President Relia 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll Free: (866)607-3542 e-mail: relia1@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia From ian.montgomery <@t> bio.gla.ac.uk Wed Jun 4 07:55:24 2008 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Wed Jun 4 07:55:37 2008 Subject: [Histonet] ATPase. Message-ID: About to trial two techniques for the demonstration of ATPase in human skeletal muscle. A Metachromatic Dye-ATPase Method for the Simultaneous Identification of Skeletal Muscle Fiber Types I, IIA, IIB and IIC. Ogilvie,R.W. & Feeback,D.L. 1990. Stain Technol. 65. 231-242 Inhibition Reactivation Myofibrillar ATPase Technique for Demonstration of Three Fiber Types in a Single Cryostat Section. Horak,V. & Matolin,S. 1990 Stain Technol. 65. 85-90 Has anyone tried these techniques and do you have any comments on their efficacy? They both appear straightforward with the papers giving details of the method. But like everything there is always the wee wrinkle that's omitted that makes the difference. So, why re-invent the wheel and give myself even more grey hair when someone might have the answer. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. From Bonnie.Whitaker <@t> osumc.edu Wed Jun 4 07:59:01 2008 From: Bonnie.Whitaker <@t> osumc.edu (Whitaker, Bonnie) Date: Wed Jun 4 07:59:14 2008 Subject: [Histonet] MLT working histology? In-Reply-To: <979FF5962E234F45B06CF0DB7C1AABB20FEC203C@chi2k3ms01.columbuschildrens.net> References: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F57A@lmhsmail.lmhealth.org> <979FF5962E234F45B06CF0DB7C1AABB20FEC203C@chi2k3ms01.columbuschildrens.net> Message-ID: <3CE20ED86C4A114EBDF3BCE8DEFD8F602624BF@msxc06.OSUMC.EDU> Hi Everyone, I wanted to chime in on this one.... I think that there are people with just about any background who will make good histotechs, and people with just about any background who will make poor histotechs. It is very important for techs to understand the theory behind the procedures that they are doing, and this can be accomplished in many ways. It generally will be better if someone has enough of a science background to already understand some of the basic principles involved in histotechnology, but there will always be the exceptional person (on both sides of the curve). Rather than focusing on whether a person is an MLT, an MT, has a biology degree, etc., it would probably be better to focus on what kind of drive that person seems to have, and whether or not they are looking for a "job" or for a "career", and make it clear that they are expected to obtain their registry and undergo regular competency assessments that should do more than "pay lipservice" to the regulations. (Of course I'm speaking for clinical labs that are CLIA and/or CAP.) Bonnie Whitaker, MT(HEW), HT(ASCP)QIHC Clinical Histology Manager Ohio State University Medical Center N308B Doan Hall 410 W. 10th Ave. Columbus, OH 43210 614.293.5048 Bonnie.Whitaker@osumc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Wednesday, June 04, 2008 8:20 AM To: Tom McNemar; jjenkins@sch-farmville.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] MLT working histology? Why couldn't she? I have an MLT (taking her certification this year) and she is far superior to many of the "old timers" who claim to be histotechs but have absolutely no theoretical knowledge and are thus hopeless when it comes to troubleshooting. This profession needs to take the bull by the horns and change the outlook of histotechnology - it is a biomedical scientific profession and the training and examinations should reflect this. It is already lagging far behind MT's. The HT examination is a joke, and the QIHC certification is not much better. Now let the flaming begin!!!!!!!!!!! Ronnie Houston, MS, HT(ASCP)QIHC, AIBMS Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 Ronald.Houston@NationwideChildrens.org www.NationwideChildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Wednesday, June 04, 2008 8:10 AM To: jjenkins@sch-farmville.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] MLT working histology? I've had 3 or 4 MLT's over the years. They were trained to do every thing the HT's do. They were hired with the stipulation that they got their HT certification as well as having their MLT certification but only because it was a requirement for all of us. They have worked out well. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jennie Jenkins Sent: Wednesday, May 28, 2008 1:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MLT working histology? Can an MLT work in histology? She would be embedding, cutting, staining, cover slipping, labeling, filing, requisitioning and setting up the gross. If possible we could have her trained to gross in anything that would not require "cutting", like GI biopsies. Jennie Jenkins, PA(ASCP) Southside Community Hospital 800 Oak Street Farmville, Virginia 23901 ================================================== This e-mail message (and attachments) may contain information that is confidential to Southside Community Hospital. If you are not the intended recipient you cannot use, distribute or copy the message or attachments. In such a case, please notify the sender by return e-mail immediately and erase all copies of the message and attachments. Opinions, conclusions and other information in this message and attachments that do not relate to the official business of Southside Community Hospital are neither given nor endorsed by it. ================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Gguerzon <@t> lifebridgehealth.org Wed Jun 4 08:08:50 2008 From: Gguerzon <@t> lifebridgehealth.org (Godfrey Guerzon) Date: Wed Jun 4 08:09:04 2008 Subject: [Histonet] MLT working histology? In-Reply-To: <3CE20ED86C4A114EBDF3BCE8DEFD8F602624BF@msxc06.OSUMC.EDU> References: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F57A@lmhsmail.lmhealth.org> <979FF5962E234F45B06CF0DB7C1AABB20FEC203C@chi2k3ms01.columbuschildrens.net> <3CE20ED86C4A114EBDF3BCE8DEFD8F602624BF@msxc06.OSUMC.EDU> Message-ID: <48465BA2.704A.0068.0@lifebridgehealth.org> You hit it right on the head Bonnie. Godfrey >>> "Whitaker, Bonnie" 6/4/2008 8:59 AM >>> Hi Everyone, I wanted to chime in on this one.... I think that there are people with just about any background who will make good histotechs, and people with just about any background who will make poor histotechs. It is very important for techs to understand the theory behind the procedures that they are doing, and this can be accomplished in many ways. It generally will be better if someone has enough of a science background to already understand some of the basic principles involved in histotechnology, but there will always be the exceptional person (on both sides of the curve). Rather than focusing on whether a person is an MLT, an MT, has a biology degree, etc., it would probably be better to focus on what kind of drive that person seems to have, and whether or not they are looking for a "job" or for a "career", and make it clear that they are expected to obtain their registry and undergo regular competency assessments that should do more than "pay lipservice" to the regulations. (Of course I'm speaking for clinical labs that are CLIA and/or CAP.) Bonnie Whitaker, MT(HEW), HT(ASCP)QIHC Clinical Histology Manager Ohio State University Medical Center N308B Doan Hall 410 W. 10th Ave. Columbus, OH 43210 614.293.5048 Bonnie.Whitaker@osumc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Wednesday, June 04, 2008 8:20 AM To: Tom McNemar; jjenkins@sch-farmville.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] MLT working histology? Why couldn't she? I have an MLT (taking her certification this year) and she is far superior to many of the "old timers" who claim to be histotechs but have absolutely no theoretical knowledge and are thus hopeless when it comes to troubleshooting. This profession needs to take the bull by the horns and change the outlook of histotechnology - it is a biomedical scientific profession and the training and examinations should reflect this. It is already lagging far behind MT's. The HT examination is a joke, and the QIHC certification is not much better. Now let the flaming begin!!!!!!!!!!! Ronnie Houston, MS, HT(ASCP)QIHC, AIBMS Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 Ronald.Houston@NationwideChildrens.org www.NationwideChildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Wednesday, June 04, 2008 8:10 AM To: jjenkins@sch-farmville.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] MLT working histology? I've had 3 or 4 MLT's over the years. They were trained to do every thing the HT's do. They were hired with the stipulation that they got their HT certification as well as having their MLT certification but only because it was a requirement for all of us. They have worked out well. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jennie Jenkins Sent: Wednesday, May 28, 2008 1:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MLT working histology? Can an MLT work in histology? She would be embedding, cutting, staining, cover slipping, labeling, filing, requisitioning and setting up the gross. If possible we could have her trained to gross in anything that would not require "cutting", like GI biopsies. Jennie Jenkins, PA(ASCP) Southside Community Hospital 800 Oak Street Farmville, Virginia 23901 ================================================== This e-mail message (and attachments) may contain information that is confidential to Southside Community Hospital. If you are not the intended recipient you cannot use, distribute or copy the message or attachments. In such a case, please notify the sender by return e-mail immediately and erase all copies of the message and attachments. Opinions, conclusions and other information in this message and attachments that do not relate to the official business of Southside Community Hospital are neither given nor endorsed by it. ================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------------------------------- THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE ADDRESSEE LISTED ABOVE. This record has been disclosed in accordance with Subtitle 3 of Title 4 of the Health-General Article of the Annotated Code of Maryland. Further disclosure of medical information contained herein is prohibited. If you are neither the intended recipient nor the individual responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure of patient information is strictly prohibited. If you have received this email in error, immediately notify us by telephone or return email. ----------------------------------------------------------------- From nancy.troiano <@t> yale.edu Wed Jun 4 08:30:12 2008 From: nancy.troiano <@t> yale.edu (Nancy W. Troiano) Date: Wed Jun 4 08:30:26 2008 Subject: [Histonet] MMA deplasticizing Message-ID: <5.2.1.1.2.20080604092735.021774b0@email.med.yale.edu> I deplastify our MMA slides using Cellosolve, available from Fisher, catalog #E181-4 - use two 30 minute room temp soaks to deplastify 5 micron thick sections - use under fume hood as fumes are hazardous. Or, alternatively, used two 5 minute soaks in acetone in a fume hood. We follow up both deplastifications with a 5 minute soak in 70% ethanol, 5 minutes in 40% ethanol then at least 5 minutes in distilled water prior to staining. From Erin.Martin <@t> ucsf.edu Wed Jun 4 08:36:40 2008 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Wed Jun 4 08:38:42 2008 Subject: [Histonet] Toluidine blue and H&E Message-ID: Bob Richmond asks: "I hope somebody does try this and report back to us all." I'll let you know how it goes - the pathologist is not working on gastric biopsies but rather prefers toluidine blue to H&E for certain derm cases. We're trying to give him both. :) From John.Auld <@t> whnt.nhs.uk Wed Jun 4 08:47:50 2008 From: John.Auld <@t> whnt.nhs.uk (John Auld) Date: Wed Jun 4 09:01:08 2008 Subject: [Histonet] Re: MLT working histology Message-ID: No flaming from me Ronnie, as a UK based Histo manager I am always surprised at discussions from the US about registration and the difference between MLT s and HTs etc. As you are aware in the UK all BioMedical Laboratory Scientists of ALL disciplines are qualified to the same level and registration is mandatory for all. As I say above I am surprised that in the more litigatous USA it is not similar. Regards John John Auld MSc, CSci, FIBMS, MCMI Service Manager Dept of Histopathology and Clinical Cytology Wirral University Teaching Hospital NHS Foundation Trust Arrowe Park Road Upton Wirral CH49 5PE Internal extn 2560 External Tel 0151 604 7025 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Wednesday, June 04, 2008 8:20 AM To: Tom McNemar; jjenkins@sch-farmville.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] MLT working histology? Why couldn't she? I have an MLT (taking her certification this year) and she is far superior to many of the "old timers" who claim to be histotechs but have absolutely no theoretical knowledge and are thus hopeless when it comes to troubleshooting. This profession needs to take the bull by the horns and change the outlook of histotechnology - it is a biomedical scientific profession and the training and examinations should reflect this. It is already lagging far behind MT's. The HT examination is a joke, and the QIHC certification is not much better. Now let the flaming begin!!!!!!!!!!! Ronnie Houston, MS, HT(ASCP)QIHC, AIBMS Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 Ronald.Houston@NationwideChildrens.org www.NationwideChildrens.org From gu.lang <@t> gmx.at Wed Jun 4 09:01:27 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Jun 4 09:01:31 2008 Subject: AW: [Histonet] Toluidine blue and H&E In-Reply-To: Message-ID: <328A1ABB103344728392FEC863B0C7D7@dielangs.at> Some time ago I tried toluidinblue alone on skin to demonstrate mastcells. It works fine, and I think (if this is your purpose) eosin would rather disturb the clear result (nuclei blue, mastcellgranula red). 0,1% Toluidinblue in 1% acetic acid 5 min Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Martin, Erin Gesendet: Mittwoch, 04. Juni 2008 15:37 An: histonet Betreff: [Histonet] Toluidine blue and H&E Bob Richmond asks: "I hope somebody does try this and report back to us all." I'll let you know how it goes - the pathologist is not working on gastric biopsies but rather prefers toluidine blue to H&E for certain derm cases. We're trying to give him both. :) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mickie25 <@t> netzero.net Wed Jun 4 09:12:04 2008 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Wed Jun 4 09:12:41 2008 Subject: [Histonet] Toluidine blue and H&E In-Reply-To: References: Message-ID: Hi Everyone, Toluidine Blue is preferred by some Mohs surgeons (if they were trained with T. Blue) for staining frozen sections of Basal Cell Carcinoma (BCC) because the collagen surrounding BCC is metachromatic. These same Mohs surgeons prefer H&E for Squamous Cell Carcinoma (SCC). Mohs surgeons trained on only H&E prefer H&E for everything. This explanation may not be relevant to this particular thread, but I thought it might be of interest. Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Erin Sent: Wednesday, June 04, 2008 6:37 AM To: histonet Subject: [Histonet] Toluidine blue and H&E Bob Richmond asks: "I hope somebody does try this and report back to us all." I'll let you know how it goes - the pathologist is not working on gastric biopsies but rather prefers toluidine blue to H&E for certain derm cases. We're trying to give him both. :) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Tracey.Lenek <@t> CLS.ab.ca Wed Jun 4 09:52:25 2008 From: Tracey.Lenek <@t> CLS.ab.ca (Tracey.Lenek@CLS.ab.ca) Date: Wed Jun 4 09:53:10 2008 Subject: [Histonet] Special Stains Kit Message-ID: <695BA3B5A811274182C175122F8663C02959FF@mail1.calgary.com> Hi, Does anyone know of a supplier for a Chloroacetate esterase kit other than Sigma? Thanks Tracey CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. From rjbuesa <@t> yahoo.com Wed Jun 4 10:10:57 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 4 10:11:01 2008 Subject: [Histonet] Re: MLT working histology In-Reply-To: Message-ID: <32760.25751.qm@web65703.mail.ac4.yahoo.com> Those are among the advantages of the UK socialized medicine and the rules of the NHS (the third largest employer in the World!) Ren? J. John Auld wrote: No flaming from me Ronnie, as a UK based Histo manager I am always surprised at discussions from the US about registration and the difference between MLT s and HTs etc. As you are aware in the UK all BioMedical Laboratory Scientists of ALL disciplines are qualified to the same level and registration is mandatory for all. As I say above I am surprised that in the more litigatous USA it is not similar. Regards John John Auld MSc, CSci, FIBMS, MCMI Service Manager Dept of Histopathology and Clinical Cytology Wirral University Teaching Hospital NHS Foundation Trust Arrowe Park Road Upton Wirral CH49 5PE Internal extn 2560 External Tel 0151 604 7025 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Wednesday, June 04, 2008 8:20 AM To: Tom McNemar; jjenkins@sch-farmville.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] MLT working histology? Why couldn't she? I have an MLT (taking her certification this year) and she is far superior to many of the "old timers" who claim to be histotechs but have absolutely no theoretical knowledge and are thus hopeless when it comes to troubleshooting. This profession needs to take the bull by the horns and change the outlook of histotechnology - it is a biomedical scientific profession and the training and examinations should reflect this. It is already lagging far behind MT's. The HT examination is a joke, and the QIHC certification is not much better. Now let the flaming begin!!!!!!!!!!! Ronnie Houston, MS, HT(ASCP)QIHC, AIBMS Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 Ronald.Houston@NationwideChildrens.org www.NationwideChildrens.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Erin.Martin <@t> ucsf.edu Wed Jun 4 10:59:26 2008 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Wed Jun 4 11:00:47 2008 Subject: [Histonet] HT practical Message-ID: Hi everyone, I have two techs who are getting ready to apply for the HT exam but I can't find any info about the practical portion of the exam. Was it discontinued? Or am I not looking in the right place on the ASCP BOR page? Thanks Erin From CEvanish <@t> mont-hosp.com Wed Jun 4 11:01:16 2008 From: CEvanish <@t> mont-hosp.com (Chris Evanish) Date: Wed Jun 4 11:01:33 2008 Subject: [Histonet] Re: Disposal of Tissue blocks In-Reply-To: <7aa56484.680@MHMC_MAIL_02.mont-hosp.com> References: <7aa56484.680@MHMC_MAIL_02.mont-hosp.com> Message-ID: <48468328.6020.00E2.0@mont-hosp.com> Can I have your inputs on the method you use to dispose of old tissue paraffin blocks >>> 6/4/2008 9:05 am >>> Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Histonet Digest, Vol 55, Issue 3 (Jerry Helisek) 2. Re: Toluidine Blue and H&E (Robert Richmond) 3. Plastic coplin Jar with Domed Lid (Ellenburg, Deborah) 4. Alternative to Histobath (Melissa Gonzalez) 5. Correlation studies (Clark Stephen - Myrtle Beach) 6. traveling (Karla Arrington) 7. Employment Opportunities (Tawanda Johnson) 8. Re: Correlation studies (Rene J Buesa) 9. xylene grades (Merced Leiker) 10. Re: Correlation studies (Joe Nocito) 11. RE: MMA de-plasticizing (Michelle McDonald) 12. Re: dome lid coplin jars (Ale? Kladnik) 13. Re: Toluidine Blue and H&E (Jim Reilly) 14. RE: Alternative to Histobath (Alan Bright) 15. GMA immunos (Edwards, R.E.) 16. RE: Creative Waste Solutions (anitathorn@comcast.net) 17. RE: MLT working histology? (Tom McNemar) 18. Re: xylene grades (Rene J Buesa) 19. RE: MLT working histology? (Houston, Ronald) 20. RELIA Special JOB Alert (Pam Barker) 21. ATPase. (Ian Montgomery) 22. RE: MLT working histology? (Whitaker, Bonnie) ---------------------------------------------------------------------- Message: 1 Date: Tue, 3 Jun 2008 13:11:46 -0400 From: "Jerry Helisek" Subject: [Histonet] RE: Histonet Digest, Vol 55, Issue 3 To: Message-ID: <3855F92002259948A66A8CA2D16E3A4F0B544E@server.ralambusa.com> Content-Type: text/plain; charset="us-ascii" Sandy: This may work for you... See: http://www.ralamb.net/product_info.php?products_id=347 Thanks ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ Message: 1 Date: Mon, 2 Jun 2008 12:19:57 -0500 From: "Harrison, Sandra C." Subject: [Histonet] dome lid coplin jars To: Message-ID: Content-Type: text/plain; charset="us-ascii" This is a small thing, but it would make my Pathology People so happy (and me too!!) if I could order some plastic coplin jars with the domed lid. Has anyone received any recently and if so, which supplier did you use and catalog number? I've tried Cardinal and Fisher with no luck. Also Electron Microscopy Sciences. They send the flat lidded, plastic coplin jar that is too deep. The problem with the flat lidded, plastic coplin jars we have received is that we can't pull slides out with just our gloved fingers, but must instead use forceps, because they are so deep. The catalogs show a picture of a domed lid, but they send you the flat lidded coplin jar. Any suggestions? Thanks, Sandy ------------------------------ Message: 2 Date: Tue, 3 Jun 2008 13:20:50 -0400 From: "Robert Richmond" Subject: [Histonet] Re: Toluidine Blue and H&E To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Erin Martin asks: >>My pathologist would like to do a combined toluidine blue and H&E. Has anyone ever done this? If so, would you mind sharing your procedure? I am guessing that I would have to use the toluidine blue after the eosin then air dry the slides since alcohol would take the blue out, but I am not sure if it would react with either the hematoxylin or the eosin.<< What an interesting idea! I suppose we're talking about staining Helicobacter in gastric biopsy specimens. I was one of the early users of toluidine blue for Helicobacter, and I've more or less followed the literature but have never found such a technique. As others have suggested, I suppose the toluidine blue would come after the H & E. Moving the slides rapidly through alcohols would probably keep the dye from washing out. Most people would probably use Diff-Quik II (or a generic equivalent) rather than preparing toluidine blue. The only way to find out is to try it! I'd want you to have good control sections to practice with, and I'd expect to work closely with the histotechnologist to get the desired result. I hope somebody does try this and report back to us all. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 3 Date: Tue, 3 Jun 2008 13:44:47 -0400 From: "Ellenburg, Deborah" Subject: [Histonet] Plastic coplin Jar with Domed Lid To: Message-ID: Content-Type: text/plain; charset=iso-8859-1 Sandy, I went online and searched for domed lid coplin jars and came up with the following. Thermo Scientific - Shandon Plastic Coplin Staining Jar - SKU: 194 Hope this helps. Debbie E. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, June 03, 2008 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 55, Issue 3 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. dome lid coplin jars (Harrison, Sandra C.) 2. RE: dome lid coplin jars (Pat.Bell@UCHSC.edu) 3. Re: dome lid coplin jars (Kathleen Roberts) 4. bausch surgical (Emily Sours) 5. enzyme histochemistry- automation (Joanne Mauger) 6. Toluidine Blue and H&E (Martin, Erin) 7. Re: Toluidine Blue and H&E (Rene J Buesa) 8. Trying to reach Tracey Lenek (Pam Barker) 9. Histological products (Myl?ne de Champlain) 10. Re: Histological products (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Mon, 2 Jun 2008 12:19:57 -0500 From: "Harrison, Sandra C." Subject: [Histonet] dome lid coplin jars To: Message-ID: Content-Type: text/plain; charset="us-ascii" This is a small thing, but it would make my Pathology People so happy (and me too!!) if I could order some plastic coplin jars with the domed lid. Has anyone received any recently and if so, which supplier did you use and catalog number? I've tried Cardinal and Fisher with no luck. Also Electron Microscopy Sciences. They send the flat lidded, plastic coplin jar that is too deep. The problem with the flat lidded, plastic coplin jars we have received is that we can't pull slides out with just our gloved fingers, but must instead use forceps, because they are so deep. The catalogs show a picture of a domed lid, but they send you the flat lidded coplin jar. Any suggestions? Thanks, Sandy ------------------------------ Message: 2 Date: Mon, 2 Jun 2008 11:23:01 -0600 From: Subject: RE: [Histonet] dome lid coplin jars To: , Message-ID: <71FCC52823941D49A4BC39B48C6E3428F3C023@java.uchsc.edu> Content-Type: text/plain; charset="US-ASCII" Try Market Lab and VWR. Pat Bell HT(ASCP) Sr. PRA Division of Medical Oncology UCHSC, Aurora, CO 303-724-3845 (lab) 303-724-3889 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C. Sent: Monday, June 02, 2008 11:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] dome lid coplin jars This is a small thing, but it would make my Pathology People so happy (and me too!!) if I could order some plastic coplin jars with the domed lid. Has anyone received any recently and if so, which supplier did you use and catalog number? I've tried Cardinal and Fisher with no luck. Also Electron Microscopy Sciences. They send the flat lidded, plastic coplin jar that is too deep. The problem with the flat lidded, plastic coplin jars we have received is that we can't pull slides out with just our gloved fingers, but must instead use forceps, because they are so deep. The catalogs show a picture of a domed lid, but they send you the flat lidded coplin jar. Any suggestions? Thanks, Sandy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Mon, 02 Jun 2008 13:41:13 -0400 From: Kathleen Roberts Subject: Re: [Histonet] dome lid coplin jars To: "Harrison, Sandra C." Cc: "'histonet@lists.utsouthwestern.edu'" Message-ID: <484430B9.9000302@rci.rutgers.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed How about MarketLab? www.marketlabinc.com Cat# ML9801 I'm about to order some myself, but before I do so, are these safe to use in the microwave? I think so, but I want to ask and be sure. -Kathy Roberts Principal Lab Technician Neurotoxicology Laboratories Dept of Pharmacology and Toxicology Ernest Mario School of Pharmacy Rutgers, the State University of NJ 41 B Gordon Rd Piscataway, NJ 08854 Harrison, Sandra C. wrote: >This is a small thing, but it would make my Pathology People so happy >(and me too!!) if I could order some plastic coplin jars with the domed >lid. Has anyone received any recently and if so, which supplier did you >use and catalog number? > >I've tried Cardinal and Fisher with no luck. Also Electron Microscopy >Sciences. They send the flat lidded, plastic coplin jar that is too >deep. > >The problem with the flat lidded, plastic coplin jars we have received >is that we can't pull slides out with just our gloved fingers, but must >instead use forceps, because they are so deep. The catalogs show a >picture of a domed lid, but they send you the flat lidded coplin jar. > >Any suggestions? > >Thanks, >Sandy >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histon > > ------------------------------ Message: 4 Date: Mon, 2 Jun 2008 13:36:54 -0400 From: "Emily Sours" Subject: [Histonet] bausch surgical To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hello We need to sharpen our Vannas scissors, which we usually do through Storz. Unfortunately, we haven't done this for about four years, and Storz is now part of Bausch Surgical. Their website doesn't have any information on where to send them and I've been on hold for ten minutes twice without reaching anyone. Does anyone here have their recent address or a non-toll free number for them? Thanks, Emily -- When you're riding in a time machine way far into the future, don't stick your elbow out the window, or it'll turn into a fossil. ------------------------------ Message: 5 Date: Mon, 02 Jun 2008 15:41:59 -0400 From: "Joanne Mauger" Subject: [Histonet] enzyme histochemistry- automation To: , Message-ID: Content-Type: text/plain; charset=US-ASCII Hi Everyone, Does anyone know of an automated platform for staining fresh(frozen) muscle slides for ATP, NADH, COX, etc.? I know it's a long shot, but I had to ask. Thanks, Jo ------------------------------ Message: 6 Date: Mon, 2 Jun 2008 13:28:15 -0700 From: "Martin, Erin" Subject: [Histonet] Toluidine Blue and H&E To: "histonet" Message-ID: Content-Type: text/plain; charset=iso-8859-1 Hi all, My pathologist would like to do a combined toluidine blue and H&E. Has anyone ever done this? If so, would you mind sharing your procedure? I am guessing that I would have to use the toluidine blue after the eosin then air dry the slides since alcohol would take the blue out, but I am not sure if it would react with either the hematoxylin or the eosin. Thank you for your help! Erin Martin ------------------------------ Message: 7 Date: Mon, 2 Jun 2008 13:43:45 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Toluidine Blue and H&E To: "Martin, Erin" , histonet Message-ID: <372902.76788.qm@web65712.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I would try it first without the hematoxylin and before the eosin. You don't have to air dry the sections, just blot them and place them in isopropanol before going to xylene. Toluidin blue works better at pH 11 that you can reach with borax before dissolving the dye in it. It works also better at 50-60?C. Hope this will help you. Ren? J. "Martin, Erin" wrote: Hi all, My pathologist would like to do a combined toluidine blue and H&E. Has anyone ever done this? If so, would you mind sharing your procedure? I am guessing that I would have to use the toluidine blue after the eosin then air dry the slides since alcohol would take the blue out, but I am not sure if it would react with either the hematoxylin or the eosin. Thank you for your help! Erin Martin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Mon, 2 Jun 2008 16:46:52 -0400 From: "Pam Barker" Subject: [Histonet] Trying to reach Tracey Lenek To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Tracey, I tried to respond to your e-mail but the address that I am replying to is bouncing back as undeliverable. The answer to your question is yes and when you get a chance please send me a new e-mail address or phone number to reach you. Thanks-Pam Thank You! Pam M. Barker President Relia 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll Free: (866)607-3542 e-mail: relia1@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia ------------------------------ Message: 9 Date: Tue, 3 Jun 2008 08:56:20 -0400 From: Myl?ne de Champlain Subject: [Histonet] Histological products To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi, I would like to know if those histological products still good even if they are expired. They still sealed in the original package. Products Expiration Consul-mount histology formulation 09-2007 Shandon xylene-substitute mountant 01-2007 Instant hematoxylin (powder) 09-2003 Thank you for your answers. Myl?ne Universit? du Qu?bec ? Rimouski _________________________________________________________________ ------------------------------ Message: 10 Date: Tue, 3 Jun 2008 06:14:56 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Histological products To: "Myl?ne" de Champlain , histonet@lists.utsouthwestern.edu Message-ID: <36234.55354.qm@web65716.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 If sealed in their original package (not exposed to air), they probably are. On the other hand, it will be always cheaper to prepare a hematoxylin solution to determine how it works, than to buy a new powder bottle. Ren? J. Myl?ne de Champlain wrote: Hi, I would like to know if those histological products still good even if they are expired. They still sealed in the original package. Products Expiration Consul-mount histology formulation 09-2007 Shandon xylene-substitute mountant 01-2007 Instant hematoxylin (powder) 09-2003 Thank you for your answers. Myl?ne Universit? du Qu?bec ? Rimouski _________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 55, Issue 3 *************************************** ________________________________________________________________________________________________________________________________ ________________________________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ------------------------------ Message: 4 Date: Tue, 3 Jun 2008 11:31:25 -0700 From: "Melissa Gonzalez" Subject: [Histonet] Alternative to Histobath To: Message-ID: <2884B897182A1D438C7BA24B9A8F94A2348005@hqsvr01mail.cgi.com> Content-Type: text/plain; charset="iso-8859-1" Anyone have any recommendations on tissue freezing units? Thanks! Melissa Melissa A. Gonz?lez Edick R&D, Cell Genesys, Inc 500 Forbes Blvd South San Francisco, CA 94080 ph (650) 266-3168 fx (650) 266-3080 www.cellgenesys.com ------------------------------ Message: 5 Date: Tue, 3 Jun 2 008 13:31:39 -0500 From: "Clark Stephen - Myrtle Beach" Subject: [Histonet] Correlation studies To: Message-ID: <28E40C736ED32341BBB2B094EFD23FC8020F070B@NASEV05.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" I need to perform correlation studies for our new Immunohistochemistry machine. The question I have is what format do I use? How do I do it? What should the test look like? Etc. Any advice would be appreciated. Thanks, Steve Clark ------------------------------ Message: 6 Date: Tue, 3 Jun 2008 12:22:36 -0700 (PDT) From: Karla Arrington Subject: [Histonet] traveling To: histonet@lists.utsouthwestern.edu Message-ID: <951179.57551.qm@web32501.mail.mud.yahoo.com> Content-Type: text/plain; charset=us-ascii Fellow Histo's: I was wondering if anyone has done the "traveling Histotech" thing? Is it worth it? Need a change of pace... Very interested! Thanks! Karla Arrington, HT(ASCP) freckles9660@yahoo.com ------------------------------ Message: 7 Date: Tue, 3 Jun 2008 15:23:53 -0400 From: "Tawanda Johnson" Subject: [Histonet] Employment Opportunities To: Message-ID: <24D22DE9E488AA43BF92A4389F2DDB1F02BB2C07@mail1.BOSTWICK.COM> Content-Type: text/plain; charset="us-ascii" Bostwick Laboratories are currently seeking experienced Histotech's for our Ohio and Connecticut locations. If you have at least 2years laboratory experience and a Bachelor in Science Degree please email me your Resume. ASCP certification preferred. ------------------------------ Message: 8 Date: Tue, 3 Jun 2008 12:59:37 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Correlation studies To: Clark Stephen - Myrtle Beach , histonet@lists.utsouthwestern.edu Message-ID: <408291.80171.qm@web65716.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Correlations are always between two sets of variables (unless they refer to multiple correlations where those sets contain more than two). I have never heard of correlating an IHC machine. What are you going to correlate it with? I do not imagine how you are going to present the problem. Are you trying to correlate your results with your IHC machine with the results in another or with some other results? If that is the case you will have to run both sets simultaneously. On the other hand those correlation tests (the strongest) have to have a normal distribution of the data, or you will have to use some type of transformation. Perhaps you could find more information in http://www.vassar.edu/lowry which is a fantastic web site to perform statistical tests. Ren? J. Clark Stephen - Myrtle Beach wrote: I need to perform correlation studies for our new Immunohistochemistry machine. The question I have is what format do I use? How do I do it? What should the test look like? Etc. Any advice would be appreciated. Thanks, Steve Clark _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Tue, 03 Jun 2008 16:59:52 -0400 From: Merced Leiker Subject: [Histonet] xylene grades To: histonet@lists.utsouthwestern.edu Message-ID: <8E75CCAA47872DF1593BF16B@bchwxp2702.ad.med.buffalo.edu> Content-Type: text/plain; charset=us-ascii; format=flowed Would the grade of xylenes used for deparaffinizing make a difference for immunostaining of certain (picky) epitopes? We are using Certified A.C.S. xylenes. What about rinsing in tap water vs. distilled water during rehydration? The reason I'm asking is that we are having issues staining for c-kit (KIT, CD117) on FFPE tissue. We've tried all different antigen retrievals, including no retrieval, and still aren't seeing any stain. Any help would be greatly appreciated. Thanks! Merced M Leiker Research Technician II 354 BRB (Lee Lab) / 140 Farber Hall (mail) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 ------------------------------ Message: 10 Date: Tue, 3 Jun 2008 18:55:08 -0500 From: "Joe Nocito" Subject: Re: [Histonet] Correlation studies To: "Clark Stephen - Myrtle Beach" , Message-ID: <003e01c8c5d5$4167d990$0302a8c0@yourxhtr8hvc4p> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Steve, if you have been performing immunos manually, you can test the machine results against the manual ones. Run slides manually and on the machine. Reagents should be the same lot number. When I switched to an automated machine, I had to test each antibody and each HIER method. I kept the records and slides for 2 CAP cycles, a bit anal, but I wanted to be sure that CAP understood that I did have a validation system. Good luck Joe ----- Original Message ----- From: "Clark Stephen - Myrtle Beach" To: Sent: Tuesday, June 03, 2008 1:31 PM Subject: [Histonet] Correlation studies I need to perform correlation studies for our new Immunohistochemistry machine. The question I have is what format do I use? How do I do it? What should the test look like? Etc. Any advice would be appreciated. Thanks, Steve Clark _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Wed, 4 Jun 2008 14:20:26 +1000 From: "Michelle McDonald" Subject: RE: [Histonet] MMA de-plasticizing To: "Michele Wich" , Message-ID: <47BD6E7614A693499453835D47E36F70045F0AD8@hedwig.nch.kids> Content-Type: text/plain; charset="iso-8859-1" Dear Michelle, We de-plasticise MMP bone sections using two changes of 100% acetone for 10 monis each. Thanks Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele Wich Sent: Saturday, 31 May 2008 1:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MMA de-plasticizing Is there anyone out there doing MMA on un-decalcified bone? I'm wondering if there is any way to de-plasticize without putting the slides in heated xylene--a potentially explosive situation which technically would require explosion proof oven, hood, clothing, etc. I know that the flash point of xylene is quite low (26.1?C, I think, which is barely above room temperature). Is there a way around this safety issue? This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and n otify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 12 Date: Wed, 04 Jun 2008 09:52:54 +0200 From: Ale? Kladnik Subject: [Histonet] Re: dome lid coplin jars To: histonet@lists.utsouthwestern.edu Message-ID: <484649D6.6080807@bf.uni-lj.si> Content-Type: text/plain; charset=UTF-8; format=flowed Try Bel-Art, they used to sell dome shaped coplin jars, now they claim they have an improved design with large opening - they even show a gloved hand taking a slide in/out of the jar. http://www.belart.com/cat/lifescience/en/442081000.asp > This is a small thing, but it would make my Pathology People so happy > (and me too!!) if I could order some plastic coplin jars with the domed > lid. Has anyone received any recently and if so, which supplier did you > use and catalog number? > > I've tried Cardinal and Fisher with no luck. Also Electron Microscopy > Sciences. They send the flat lidded, plastic coplin jar that is too > deep. > > The problem with the flat lidded, plastic coplin jars we have received > is that we can't pull slides out with just our gloved fingers, but must > instead use forceps, because they are so deep. The catalogs show a > picture of a domed lid, but they send you the flat lidded coplin jar. > > Any suggestions? > > Thanks, > Sandy -- Ale?? Kladnik Univerza v Ljubljani, Biotehni??ka fakulteta, Oddelek za biologijo Ve? na pot 111, SI-1000 Ljubljana, Slovenija tel: +386 1 4233388, fax: +386 1 2573390 url: http://botanika.biologija.org/kdosmo/aleskl.php url: http://web.bf.uni-lj.si/bi/mikroskopija/ ------------------------------ Message: 13 Date: Wed, 4 Jun 2008 09:46:12 +0100 From: "Jim Reilly" Subject: [Histonet] Re: Toluidine Blue and H&E To: Message-ID: <82B54C1E04D94D489448735E6CBDE4606DC2B5@exchange-be5.centre.ad.gla.ac.uk> Content-Type: text/plain; charset="US-ASCII" Hello I Tried Toluidine Blue / Eosin on decalcified mouse footpads : 2 minutes Tol Blue 1 minute Eosin rapid dehydration in 95% alcohol x1 100% x2 (10 dips each) clear in xylene and mount in DPX. This seemed to work OK. Jim Reilly Glasgow Biomedical Research Centre ------------------------------ Message: 14 Date: Wed, 4 Jun 2008 11:39:23 +0100 From: "Alan Bright" Subject: RE: [Histonet] Alternative to Histobath To: "Melissa Gonzalez" , Message-ID: Content-Type: text/plain; charset="iso-8859-1" Dear Melissa, Yes, we manufacture the Clini-RF Rapid Freezer which goes down to -80 degs. (See our Web site) C. We also fit as an option a Quick Freezer into our OTF5000 Cryostat. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype: dazzle0 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Melissa Gonzalez Sent: 03 June 2008 19:31 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Alternative to Histobath Anyone have any recommendations on tissue freezing units? Thanks! Melissa Melissa A. Gonz?lez Edick R&D, Cel l Genesys, Inc 500 Forbes Blvd South San Francisco, CA 94080 ph (650) 266-3168 fx (650) 266-3080 www.cellgenesys.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Wed, 4 Jun 2008 12:33:23 +0100 From: "Edwards, R.E." Subject: [Histonet] GMA immunos To: "histonet@lists.utsouthwestern.edu" Message-ID: <7722595275A4DD4FA225B92CDBF174A172A577C87E@EXC-MBX3.cfs.le.ac.uk> Content-Type: text/plain; charset="us-ascii" After a lifetime of immunostaining paraffin wax sections, in my new job I am having to deal with GMA sections. Two questions, firstly, does one have to float out the sections for a minimum of 90 seconds on 0.5% ammonium hydroxide before mounting on a slide;( with the ammonaical water apparently acting as some sort of antigen retrieval step), and immunohistochemistry on GMA sections does not work if one uses PBS as opposed to TBS. Any help/hints gratefully received. Cheers Richard Edwards University of Leicester. U.K. ------------------------------ Message: 16 Date: Wed, 04 Jun 2008 12:06:16 +0000 From: anitathorn@comcast.net Subject: RE: [Histonet] Creative Waste Solutions To: "Laurie Colbert" , "Angela Bitting" , Message-ID: <060420081206.14063.4846853800058ACF000036EF2200750330029D01089B0E9B07020E@comcast.net> I work in a small volume lab and find that both the formalin and alcohol recycling systems are perfect for our needs. Easy to use and very economical. -------------- Original message ---------------------- From: "Laurie Colbert" > Angie, > > We use their formalin recycler, and we love it. It is very easy to use. > > Laurie Colbert > Huntington Hospital > Pasadena, CA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela > Bitting > Sent: Monday, June 02, 2008 4:24 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Creative Waste Solutions > > Would users of recycling products from this company share feedback with > me? > > Thanks, > Angie > > Angela Bitting, HT(ASCP) > Technical Specialist, Histology > Geisinger Medical Center > 100 N Academy Ave. MC 23-00 > Danville, PA 17822 > phone 570-214-9634 > fax 570-271-5916 > > No trees were hurt in the sending of this email > However many electrons were severly inconvienienced! > > > > > IMPORTANT WARNING: The information in this message (and the documents > attached to it, if any) is confidential and may be legally privileged. > It is intended solely for the addressee. Access to this message by > anyone else is unauthorized. If you are not the intended recipient, any > disclosure, copying, distribution or any action taken, or omitted to be > taken, in reliance on it is prohibited and may be unlawful. If you have > received this message in error, please delete all electronic copies of > this message (and the documents attached to it, if any), destroy any > hard copies you may have created and notify me immediately by replying > to this email. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Wed, 4 Jun 2008 08:09:56 -0400 From: "Tom McNemar" Subject: RE: [Histonet] ML T working histology? To: , Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F57A@lmhsmail.lmhealth.org> Content-Type: text/plain; charset="iso-8859-1" I've had 3 or 4 MLT's over the years. They were trained to do every thing the HT's do. They were hired with the stipulation that they got their HT certification as well as having their MLT certification but only because it was a requirement for all of us. They have worked out well. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jennie Jenkins Sent: Wednesday, May 28, 2008 1:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MLT working histology? Can an MLT work in histology? She would be embedding, cutting, staining, cover slipping, labeling, filing, requisitioning and setting up the gross. If possible we could have her trained to gross in anything that would not require "cutting", like GI biopsies. Jennie Jenkins, PA(ASCP) Southside Community Hospital 800 Oak Street Farmville, Virginia 23901 ================================================== This e-mail message (and attachments) may contain information that is confidential to Southside Community Hospital. If you are not the intended recipient you cannot use, distribute or copy the message or attachments. In such a case, please notify the sender by return e-mail immediately and erase all copies of the message and attachments. Opinions, conclusions and other information in this message and attachments that do not relate to the official business of Southside Community Hospital are neither given nor endorsed by it. ================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Wed, 4 Jun 2008 05:12:24 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] xylene grades To: Merced Leiker , histonet@lists.utsouthwestern.edu Message-ID: <472664.60493.qm@web65713.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 The xylene "grade" (if such a thing exists!) is of a lesser concern than assuring a complete dewaxing of the section. You have to completely eliminate all the paraffin. Using distilled water to later immerse the sections in a buffer (that contains salts) is of no relevance, because you are going to "salinize" the sections any way. I think that your problem is with your Ab supplier, or the lot. CD117 is a "tricky" one. I stopped having problems when I switched to DAKO Mo Ab and HIER at pH6 Ren? J. Merced Leiker wrote: Would the grade of xylenes used for deparaffinizing make a difference for immunostaining of certain (picky) epitopes? We are using Certified A.C.S. xylenes. What about rinsing in tap water vs. distilled water during rehydration? The reason I'm asking is that we are having issues staining for c-kit (KIT, CD117) on FFPE tissue. We've tried all different antigen retrievals, including no retrieval, and still aren't seeing any stain. Any help would be greatly appreciated. Thanks! Merced M Leiker Research Technician II 354 BRB (Lee Lab) / 140 Farber Hall (mail) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Wed, 4 Jun 2008 08:20:19 - 0400 From: "Houston, Ronald" Subject: RE: [Histonet] MLT working histology? To: "Tom McNemar" , , Message-ID: <979FF5962E234F45B06CF0DB7C1AABB20FEC203C@chi2k3ms01.columbuschildrens.net> Content-Type: text/plain; charset="us-ascii" Why couldn't she? I have an MLT (taking her certification this year) and she is far superior to many of the "old timers" who claim to be histotechs but have absolutely no theoretical knowledge and are thus hopeless when it comes to troubleshooting. This profession needs to take the bull by the horns and change the outlook of histotechnology - it is a biomedical scientific profession and the training and examinations should reflect this. It is already lagging far behind MT's. The HT examination is a joke, and the QIHC certification is not much better. Now let the flaming begin!!!!!!!!!!! Ronnie Houston, MS, HT(ASCP)QIHC, AIBMS Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 Ronald.Houston@NationwideChildrens.org www.NationwideChildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Wednesday, June 04, 2008 8:10 AM To: jjenkins@sch-farmville.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] MLT working histology? I've had 3 or 4 MLT's over the years. They were trained to do every thing the HT's do. They were hired with the stipulation that they got their HT certification as well as having their MLT certification but only because it was a requirement for all of us. They have worked out well. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jennie Jenkins Sent: Wednesday, May 28, 2008 1:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MLT working histology? Can an MLT work in histology? She would be embedding, cutting, staining, cover slipping, labeling, filing, requisitioning and setting up the gross. If possible we could have her trained to gross in anything that would not require "cutting", like GI biopsies. Jennie Jenkins, PA(ASCP) Southside Community Hospital 800 Oak Street Farmville, Virginia 23901 ================================================== This e-mail message (and attachments) may contain information that is confidential to Southside Community Hospital. If you are not the intended recipient you cannot use, distribute or copy the message or attachments. In such a case, please notify the sender by return e-mail immediately and erase all copies of the message and attachments. Opinions, conclusions and other information in this message and attachments that do not relate to the official business of Southside Community Hospital are neither given nor endorsed by it. ================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ------------------------------ Message: 20 Date: Wed, 4 Jun 2008 08:48:41 -0400 From: "Pam Barker" Subject: [Histonet] RELIA Special JOB Alert To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Histonetters! I had one more position come in that I am pretty excited about. I have a client in the Philadelphia area in need of a senior histotechnologist. They are looking for someone who is ASCP certified with at least 5 years of experience. My client offers excellent compensation, benefits and a great place to work. Dermatopathology experience is a plus. If you are interested please contact me. I can be reached at relia1@earthlink.net or toll free at 866-607-3542. If you know someone who might be interested please feel free to pass the information along to them as well. Incidentally if you happen to know an ASCP certified med tech with microbiology experience I have a full time permanent 1st shift position in Cincinnati. Thanks and have a great day!! - Pam Thank You! Pam M. Barker President Relia 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll Free: (866)607-3542 e-mail: relia1@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia ------------------------------ Message: 21 Date: Wed, 4 Jun 2008 13:55:24 +0100 From: "Ian Montgomery" Subject: [Histonet] ATPase. To: Message-ID: Content-Type: text/plain; charset="us-ascii" About to trial two techniques for the demonstration of ATPase in human skeletal muscle. A Metachromatic Dye-ATPase Method for the Simultaneous Identification of Skeletal Muscle Fiber Types I, IIA, IIB and IIC. Ogilvie,R.W. & Feeback,D.L. 1990. Stain Technol. 65. 231-242 Inhibition Reactivation Myofibrillar ATPase Technique for Demonstration of Three Fiber Types in a Single Cryostat Section. Horak,V. & Matolin,S. 1990 Stain Technol. 65. 85-90 Has anyone tried these techniques and do you have any comments on their efficacy? They both appear straightforward with the papers giving details of the method. But like everything there is always the wee wrinkle that's omitted that makes the difference. So, why re-invent the wheel and give myself even more grey hair when someone might have the answer. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. ------------------------------ Message: 22 Date: Wed, 4 Jun 2008 08:59:01 -0400 From: "Whitaker, Bonnie" Subject: RE: [Histonet] MLT working histology? To: histonet@lists.utsouthwestern.edu Message-ID: <3CE20ED86C4A114EBDF3BCE8DEFD8F602624BF@msxc06.OSUMC.EDU> Content-Type: text/plain; charset=us-ascii Hi Everyone, I wanted to chime in on this one.... I think that there are people with just about any background who will make good histotechs, and people with just about any background who will make poor histotechs. It is very important for techs to understand the theory behind the procedures that they are doing, and this can be accomplished in many ways. It generally will be better if someone has enough of a science background to already understand some of the basic principles involved in histotechnology, but there will always be the exceptional person (on both sides of the curve). Rather than focusing on whether a person is an MLT, an MT, has a biology degree, etc., it would probably be better to focus on what kind of drive that person seems to have, and whether or not they are looking for a "job" or for a "career", and make it clear that they are expected to obtain their registry and undergo regular competency assessments that should do more than "pay lipservice" to the regulations. (Of course I'm speaking for clinical labs that are CLIA and/or CAP.) Bonnie Whitaker, MT(HEW), HT(ASCP)QIHC Clinical Histology Manager Ohio State University Medical Center N308B Doan Hall 410 W. 10th Ave. Columbus, OH 43210 614.293.5048 Bonnie.Whitaker@osumc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Wednesday, June 04, 2008 8:20 AM To: Tom McNemar; jjenkins@sch-farmville.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] MLT working histology? Why couldn't she? I have an MLT (taking her certification this year) and she is far superior to many of the "old timers" who claim to be histotechs but have absolutely no theoretical knowledge and are thus hopeless when it comes to troubleshooting. This profession needs to take the bull by the horns and change the outlook of histotechnology - it is a biomedical scientific profession and the training and examinations should reflect this. It is already lagging far behind MT's. The HT examination is a joke, and the QIHC certification is not much better. Now let the flaming begin!!!!!!!!!!! Ronnie Houston, MS, HT(ASCP)QIHC, AIBMS Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 Ronald.Houston@NationwideChildrens.org www.NationwideChildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Wednesday, June 04, 2008 8:10 AM To: jjenkins@sch-farmville.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] MLT working histology? I've had 3 or 4 MLT's over the years. They were trained to do every thing the HT's do. They were hired with the stipulation that they got their HT certification as well as having their MLT certification but only because it was a requirement for all of us. They have worked out well. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jennie Jenkins Sent: Wednesday, May 28, 2008 1:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MLT working histology? Can an MLT work in histology? She would be embedding, cutting, staining, cover slipping, labeling, filing, requisitioning and setting up the gross. If possible we could have her trained to gross in anything that would not require "cutting", like GI biopsies. Jennie Jenkins, PA(ASCP) Southside Community Hospital 800 Oak Street Farmville, Virginia 23901 ================================================== This e-mail message (and attachments) may contain information that is confidential to Southside Community Hospital. If you are not the intended recipient you cannot use, distribute or copy the message or attachments. In such a case, please notify the sender by return e-mail immediately and erase all copies of the message and attachments. Opinions, conclusions and other information in this message and attachments that do not relate to the official business of Southside Community Hospital are neither given nor endorsed by it. ================================================== _______________________________________________ Histonet mailin g list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 55, Issue 4 *************************************** From RSRICHMOND <@t> aol.com Wed Jun 4 11:05:02 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Wed Jun 4 11:05:09 2008 Subject: [Histonet] Re: Alternative to Histobath Message-ID: Replacement for the Histobath rapid freezing unit, apparently no longer in manufacture: Alan Bright, at Bright Instrument Co. in England, notes that >>we manufacture the Clini-RF Rapid Freezer which goes down to -80 degs. (See our Web site) C. We also fit as an option a Quick Freezer into our OTF5000 Cryostat.<< Histobath consisted of a container of liquid cooled to a low temperature by a freezer. The open pot was filled with acetone (or 2-methylbutane), and I thought it was an explosion hazard even at the very low temperature the liquid was held at. At brightinstrument.com I read that the liquid used in it was hexane, also a fire hazard in an open container. I understood that some people were using a hydrofluoroether in the Histobath. Can this be used in the Clini-RF unit? The information I had about this stuff was: 3M? Novec? Engineered Fluid HFE-7100 This product belongs to a class of fluorocarbons called "segregated hydrofluoroethers (HFE's)" According to various MSDS, HFE-7100 is methyl nonafluoroisobutyl ether C4F9-O-CH3 It melts and freezes at -135 C, so that it would remain liquid in the Histobath. (It would freeze solid in liquid nitrogen, however.) It boils at 60 C., and is listed as non-flammable. It cost around $230 a gallon a few years ago. Bob Richmond Samurai Pathologist Knoxville TN From JMacDonald <@t> mtsac.edu Wed Jun 4 11:07:15 2008 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Jun 4 11:07:21 2008 Subject: [Histonet] HT practical In-Reply-To: Message-ID: The practical portion was discontinued. Like all other ASCP exams it is the CAT exam only. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Martin, Erin" Sent by: histonet-bounces@lists.utsouthwestern.edu 06/04/2008 09:05 AM To "histonet" cc Subject [Histonet] HT practical Hi everyone, I have two techs who are getting ready to apply for the HT exam but I can't find any info about the practical portion of the exam. Was it discontinued? Or am I not looking in the right place on the ASCP BOR page? Thanks Erin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mickie25 <@t> netzero.net Wed Jun 4 11:16:20 2008 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Wed Jun 4 11:16:27 2008 Subject: [Histonet] Re: Disposal of Tissue blocks In-Reply-To: <48468328.6020.00E2.0@mont-hosp.com> References: <7aa56484.680@MHMC_MAIL_02.mont-hosp.com> <48468328.6020.00E2.0@mont-hosp.com> Message-ID: We sent them to an incinerator through bio-hazard trash pick-up when I was supervising our hospital lab. Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chris Evanish Sent: Wednesday, June 04, 2008 9:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Disposal of Tissue blocks Can I have your inputs on the method you use to dispose of old tissue paraffin blocks >>> 6/4/2008 9:05 am >>> Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Histonet Digest, Vol 55, Issue 3 (Jerry Helisek) 2. Re: Toluidine Blue and H&E (Robert Richmond) 3. Plastic coplin Jar with Domed Lid (Ellenburg, Deborah) 4. Alternative to Histobath (Melissa Gonzalez) 5. Correlation studies (Clark Stephen - Myrtle Beach) 6. traveling (Karla Arrington) 7. Employment Opportunities (Tawanda Johnson) 8. Re: Correlation studies (Rene J Buesa) 9. xylene grades (Merced Leiker) 10. Re: Correlation studies (Joe Nocito) 11. RE: MMA de-plasticizing (Michelle McDonald) 12. Re: dome lid coplin jars (Ale? Kladnik) 13. Re: Toluidine Blue and H&E (Jim Reilly) 14. RE: Alternative to Histobath (Alan Bright) 15. GMA immunos (Edwards, R.E.) 16. RE: Creative Waste Solutions (anitathorn@comcast.net) 17. RE: MLT working histology? (Tom McNemar) 18. Re: xylene grades (Rene J Buesa) 19. RE: MLT working histology? (Houston, Ronald) 20. RELIA Special JOB Alert (Pam Barker) 21. ATPase. (Ian Montgomery) 22. RE: MLT working histology? (Whitaker, Bonnie) ---------------------------------------------------------------------- Message: 1 Date: Tue, 3 Jun 2008 13:11:46 -0400 From: "Jerry Helisek" Subject: [Histonet] RE: Histonet Digest, Vol 55, Issue 3 To: Message-ID: <3855F92002259948A66A8CA2D16E3A4F0B544E@server.ralambusa.com> Content-Type: text/plain; charset="us-ascii" Sandy: This may work for you... See: http://www.ralamb.net/product_info.php?products_id=347 Thanks ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ Message: 1 Date: Mon, 2 Jun 2008 12:19:57 -0500 From: "Harrison, Sandra C." Subject: [Histonet] dome lid coplin jars To: Message-ID: Content-Type: text/plain; charset="us-ascii" This is a small thing, but it would make my Pathology People so happy (and me too!!) if I could order some plastic coplin jars with the domed lid. Has anyone received any recently and if so, which supplier did you use and catalog number? I've tried Cardinal and Fisher with no luck. Also Electron Microscopy Sciences. They send the flat lidded, plastic coplin jar that is too deep. The problem with the flat lidded, plastic coplin jars we have received is that we can't pull slides out with just our gloved fingers, but must instead use forceps, because they are so deep. The catalogs show a picture of a domed lid, but they send you the flat lidded coplin jar. Any suggestions? Thanks, Sandy ------------------------------ Message: 2 Date: Tue, 3 Jun 2008 13:20:50 -0400 From: "Robert Richmond" Subject: [Histonet] Re: Toluidine Blue and H&E To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Erin Martin asks: >>My pathologist would like to do a combined toluidine blue and H&E. Has anyone ever done this? If so, would you mind sharing your procedure? I am guessing that I would have to use the toluidine blue after the eosin then air dry the slides since alcohol would take the blue out, but I am not sure if it would react with either the hematoxylin or the eosin.<< What an interesting idea! I suppose we're talking about staining Helicobacter in gastric biopsy specimens. I was one of the early users of toluidine blue for Helicobacter, and I've more or less followed the literature but have never found such a technique. As others have suggested, I suppose the toluidine blue would come after the H & E. Moving the slides rapidly through alcohols would probably keep the dye from washing out. Most people would probably use Diff-Quik II (or a generic equivalent) rather than preparing toluidine blue. The only way to find out is to try it! I'd want you to have good control sections to practice with, and I'd expect to work closely with the histotechnologist to get the desired result. I hope somebody does try this and report back to us all. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 3 Date: Tue, 3 Jun 2008 13:44:47 -0400 From: "Ellenburg, Deborah" Subject: [Histonet] Plastic coplin Jar with Domed Lid To: Message-ID: Content-Type: text/plain; charset=iso-8859-1 Sandy, I went online and searched for domed lid coplin jars and came up with the following. Thermo Scientific - Shandon Plastic Coplin Staining Jar - SKU: 194 Hope this helps. Debbie E. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, June 03, 2008 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 55, Issue 3 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. dome lid coplin jars (Harrison, Sandra C.) 2. RE: dome lid coplin jars (Pat.Bell@UCHSC.edu) 3. Re: dome lid coplin jars (Kathleen Roberts) 4. bausch surgical (Emily Sours) 5. enzyme histochemistry- automation (Joanne Mauger) 6. Toluidine Blue and H&E (Martin, Erin) 7. Re: Toluidine Blue and H&E (Rene J Buesa) 8. Trying to reach Tracey Lenek (Pam Barker) 9. Histological products (Myl?ne de Champlain) 10. Re: Histological products (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Mon, 2 Jun 2008 12:19:57 -0500 From: "Harrison, Sandra C." Subject: [Histonet] dome lid coplin jars To: Message-ID: Content-Type: text/plain; charset="us-ascii" This is a small thing, but it would make my Pathology People so happy (and me too!!) if I could order some plastic coplin jars with the domed lid. Has anyone received any recently and if so, which supplier did you use and catalog number? I've tried Cardinal and Fisher with no luck. Also Electron Microscopy Sciences. They send the flat lidded, plastic coplin jar that is too deep. The problem with the flat lidded, plastic coplin jars we have received is that we can't pull slides out with just our gloved fingers, but must instead use forceps, because they are so deep. The catalogs show a picture of a domed lid, but they send you the flat lidded coplin jar. Any suggestions? Thanks, Sandy ------------------------------ Message: 2 Date: Mon, 2 Jun 2008 11:23:01 -0600 From: Subject: RE: [Histonet] dome lid coplin jars To: , Message-ID: <71FCC52823941D49A4BC39B48C6E3428F3C023@java.uchsc.edu> Content-Type: text/plain; charset="US-ASCII" Try Market Lab and VWR. Pat Bell HT(ASCP) Sr. PRA Division of Medical Oncology UCHSC, Aurora, CO 303-724-3845 (lab) 303-724-3889 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C. Sent: Monday, June 02, 2008 11:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] dome lid coplin jars This is a small thing, but it would make my Pathology People so happy (and me too!!) if I could order some plastic coplin jars with the domed lid. Has anyone received any recently and if so, which supplier did you use and catalog number? I've tried Cardinal and Fisher with no luck. Also Electron Microscopy Sciences. They send the flat lidded, plastic coplin jar that is too deep. The problem with the flat lidded, plastic coplin jars we have received is that we can't pull slides out with just our gloved fingers, but must instead use forceps, because they are so deep. The catalogs show a picture of a domed lid, but they send you the flat lidded coplin jar. Any suggestions? Thanks, Sandy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Mon, 02 Jun 2008 13:41:13 -0400 From: Kathleen Roberts Subject: Re: [Histonet] dome lid coplin jars To: "Harrison, Sandra C." Cc: "'histonet@lists.utsouthwestern.edu'" Message-ID: <484430B9.9000302@rci.rutgers.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed How about MarketLab? www.marketlabinc.com Cat# ML9801 I'm about to order some myself, but before I do so, are these safe to use in the microwave? I think so, but I want to ask and be sure. -Kathy Roberts Principal Lab Technician Neurotoxicology Laboratories Dept of Pharmacology and Toxicology Ernest Mario School of Pharmacy Rutgers, the State University of NJ 41 B Gordon Rd Piscataway, NJ 08854 Harrison, Sandra C. wrote: >This is a small thing, but it would make my Pathology People so happy >(and me too!!) if I could order some plastic coplin jars with the domed >lid. Has anyone received any recently and if so, which supplier did you >use and catalog number? > >I've tried Cardinal and Fisher with no luck. Also Electron Microscopy >Sciences. They send the flat lidded, plastic coplin jar that is too >deep. > >The problem with the flat lidded, plastic coplin jars we have received >is that we can't pull slides out with just our gloved fingers, but must >instead use forceps, because they are so deep. The catalogs show a >picture of a domed lid, but they send you the flat lidded coplin jar. > >Any suggestions? > >Thanks, >Sandy >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histon > > ------------------------------ Message: 4 Date: Mon, 2 Jun 2008 13:36:54 -0400 From: "Emily Sours" Subject: [Histonet] bausch surgical To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hello We need to sharpen our Vannas scissors, which we usually do through Storz. Unfortunately, we haven't done this for about four years, and Storz is now part of Bausch Surgical. Their website doesn't have any information on where to send them and I've been on hold for ten minutes twice without reaching anyone. Does anyone here have their recent address or a non-toll free number for them? Thanks, Emily -- When you're riding in a time machine way far into the future, don't stick your elbow out the window, or it'll turn into a fossil. ------------------------------ Message: 5 Date: Mon, 02 Jun 2008 15:41:59 -0400 From: "Joanne Mauger" Subject: [Histonet] enzyme histochemistry- automation To: , Message-ID: Content-Type: text/plain; charset=US-ASCII Hi Everyone, Does anyone know of an automated platform for staining fresh(frozen) muscle slides for ATP, NADH, COX, etc.? I know it's a long shot, but I had to ask. Thanks, Jo ------------------------------ Message: 6 Date: Mon, 2 Jun 2008 13:28:15 -0700 From: "Martin, Erin" Subject: [Histonet] Toluidine Blue and H&E To: "histonet" Message-ID: Content-Type: text/plain; charset=iso-8859-1 Hi all, My pathologist would like to do a combined toluidine blue and H&E. Has anyone ever done this? If so, would you mind sharing your procedure? I am guessing that I would have to use the toluidine blue after the eosin then air dry the slides since alcohol would take the blue out, but I am not sure if it would react with either the hematoxylin or the eosin. Thank you for your help! Erin Martin ------------------------------ Message: 7 Date: Mon, 2 Jun 2008 13:43:45 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Toluidine Blue and H&E To: "Martin, Erin" , histonet Message-ID: <372902.76788.qm@web65712.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I would try it first without the hematoxylin and before the eosin. You don't have to air dry the sections, just blot them and place them in isopropanol before going to xylene. Toluidin blue works better at pH 11 that you can reach with borax before dissolving the dye in it. It works also better at 50-60?C. Hope this will help you. Ren? J. "Martin, Erin" wrote: Hi all, My pathologist would like to do a combined toluidine blue and H&E. Has anyone ever done this? If so, would you mind sharing your procedure? I am guessing that I would have to use the toluidine blue after the eosin then air dry the slides since alcohol would take the blue out, but I am not sure if it would react with either the hematoxylin or the eosin. Thank you for your help! Erin Martin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Mon, 2 Jun 2008 16:46:52 -0400 From: "Pam Barker" Subject: [Histonet] Trying to reach Tracey Lenek To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Tracey, I tried to respond to your e-mail but the address that I am replying to is bouncing back as undeliverable. The answer to your question is yes and when you get a chance please send me a new e-mail address or phone number to reach you. Thanks-Pam Thank You! Pam M. Barker President Relia 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll Free: (866)607-3542 e-mail: relia1@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia ------------------------------ Message: 9 Date: Tue, 3 Jun 2008 08:56:20 -0400 From: Myl?ne de Champlain Subject: [Histonet] Histological products To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi, I would like to know if those histological products still good even if they are expired. They still sealed in the original package. Products Expiration Consul-mount histology formulation 09-2007 Shandon xylene-substitute mountant 01-2007 Instant hematoxylin (powder) 09-2003 Thank you for your answers. Myl?ne Universit? du Qu?bec ? Rimouski _________________________________________________________________ ------------------------------ Message: 10 Date: Tue, 3 Jun 2008 06:14:56 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Histological products To: "Myl?ne" de Champlain , histonet@lists.utsouthwestern.edu Message-ID: <36234.55354.qm@web65716.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 If sealed in their original package (not exposed to air), they probably are. On the other hand, it will be always cheaper to prepare a hematoxylin solution to determine how it works, than to buy a new powder bottle. Ren? J. Myl?ne de Champlain wrote: Hi, I would like to know if those histological products still good even if they are expired. They still sealed in the original package. Products Expiration Consul-mount histology formulation 09-2007 Shandon xylene-substitute mountant 01-2007 Instant hematoxylin (powder) 09-2003 Thank you for your answers. Myl?ne Universit? du Qu?bec ? Rimouski _________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 55, Issue 3 *************************************** ____________________________________________________________________________ ____________________________________________________ ____________________________________________________________________________ ____________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ------------------------------ Message: 4 Date: Tue, 3 Jun 2008 11:31:25 -0700 From: "Melissa Gonzalez" Subject: [Histonet] Alternative to Histobath To: Message-ID: <2884B897182A1D438C7BA24B9A8F94A2348005@hqsvr01mail.cgi.com> Content-Type: text/plain; charset="iso-8859-1" Anyone have any recommendations on tissue freezing units? Thanks! Melissa Melissa A. Gonz?lez Edick R&D, Cell Genesys, Inc 500 Forbes Blvd South San Francisco, CA 94080 ph (650) 266-3168 fx (650) 266-3080 www.cellgenesys.com ------------------------------ Message: 5 Date: Tue, 3 Jun 2 008 13:31:39 -0500 From: "Clark Stephen - Myrtle Beach" Subject: [Histonet] Correlation studies To: Message-ID: <28E40C736ED32341BBB2B094EFD23FC8020F070B@NASEV05.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" I need to perform correlation studies for our new Immunohistochemistry machine. The question I have is what format do I use? How do I do it? What should the test look like? Etc. Any advice would be appreciated. Thanks, Steve Clark ------------------------------ Message: 6 Date: Tue, 3 Jun 2008 12:22:36 -0700 (PDT) From: Karla Arrington Subject: [Histonet] traveling To: histonet@lists.utsouthwestern.edu Message-ID: <951179.57551.qm@web32501.mail.mud.yahoo.com> Content-Type: text/plain; charset=us-ascii Fellow Histo's: I was wondering if anyone has done the "traveling Histotech" thing? Is it worth it? Need a change of pace... Very interested! Thanks! Karla Arrington, HT(ASCP) freckles9660@yahoo.com ------------------------------ Message: 7 Date: Tue, 3 Jun 2008 15:23:53 -0400 From: "Tawanda Johnson" Subject: [Histonet] Employment Opportunities To: Message-ID: <24D22DE9E488AA43BF92A4389F2DDB1F02BB2C07@mail1.BOSTWICK.COM> Content-Type: text/plain; charset="us-ascii" Bostwick Laboratories are currently seeking experienced Histotech's for our Ohio and Connecticut locations. If you have at least 2years laboratory experience and a Bachelor in Science Degree please email me your Resume. ASCP certification preferred. ------------------------------ Message: 8 Date: Tue, 3 Jun 2008 12:59:37 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Correlation studies To: Clark Stephen - Myrtle Beach , histonet@lists.utsouthwestern.edu Message-ID: <408291.80171.qm@web65716.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Correlations are always between two sets of variables (unless they refer to multiple correlations where those sets contain more than two). I have never heard of correlating an IHC machine. What are you going to correlate it with? I do not imagine how you are going to present the problem. Are you trying to correlate your results with your IHC machine with the results in another or with some other results? If that is the case you will have to run both sets simultaneously. On the other hand those correlation tests (the strongest) have to have a normal distribution of the data, or you will have to use some type of transformation. Perhaps you could find more information in http://www.vassar.edu/lowry which is a fantastic web site to perform statistical tests. Ren? J. Clark Stephen - Myrtle Beach wrote: I need to perform correlation studies for our new Immunohistochemistry machine. The question I have is what format do I use? How do I do it? What should the test look like? Etc. Any advice would be appreciated. Thanks, Steve Clark _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Tue, 03 Jun 2008 16:59:52 -0400 From: Merced Leiker Subject: [Histonet] xylene grades To: histonet@lists.utsouthwestern.edu Message-ID: <8E75CCAA47872DF1593BF16B@bchwxp2702.ad.med.buffalo.edu> Content-Type: text/plain; charset=us-ascii; format=flowed Would the grade of xylenes used for deparaffinizing make a difference for immunostaining of certain (picky) epitopes? We are using Certified A.C.S. xylenes. What about rinsing in tap water vs. distilled water during rehydration? The reason I'm asking is that we are having issues staining for c-kit (KIT, CD117) on FFPE tissue. We've tried all different antigen retrievals, including no retrieval, and still aren't seeing any stain. Any help would be greatly appreciated. Thanks! Merced M Leiker Research Technician II 354 BRB (Lee Lab) / 140 Farber Hall (mail) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 ------------------------------ Message: 10 Date: Tue, 3 Jun 2008 18:55:08 -0500 From: "Joe Nocito" Subject: Re: [Histonet] Correlation studies To: "Clark Stephen - Myrtle Beach" , Message-ID: <003e01c8c5d5$4167d990$0302a8c0@yourxhtr8hvc4p> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Steve, if you have been performing immunos manually, you can test the machine results against the manual ones. Run slides manually and on the machine. Reagents should be the same lot number. When I switched to an automated machine, I had to test each antibody and each HIER method. I kept the records and slides for 2 CAP cycles, a bit anal, but I wanted to be sure that CAP understood that I did have a validation system. Good luck Joe ----- Original Message ----- From: "Clark Stephen - Myrtle Beach" To: Sent: Tuesday, June 03, 2008 1:31 PM Subject: [Histonet] Correlation studies I need to perform correlation studies for our new Immunohistochemistry machine. The question I have is what format do I use? How do I do it? What should the test look like? Etc. Any advice would be appreciated. Thanks, Steve Clark _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Wed, 4 Jun 2008 14:20:26 +1000 From: "Michelle McDonald" Subject: RE: [Histonet] MMA de-plasticizing To: "Michele Wich" , Message-ID: <47BD6E7614A693499453835D47E36F70045F0AD8@hedwig.nch.kids> Content-Type: text/plain; charset="iso-8859-1" Dear Michelle, We de-plasticise MMP bone sections using two changes of 100% acetone for 10 monis each. Thanks Michelle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele Wich Sent: Saturday, 31 May 2008 1:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MMA de-plasticizing Is there anyone out there doing MMA on un-decalcified bone? I'm wondering if there is any way to de-plasticize without putting the slides in heated xylene--a potentially explosive situation which technically would require explosion proof oven, hood, clothing, etc. I know that the flash point of xylene is quite low (26.1?C, I think, which is barely above room temperature). Is there a way around this safety issue? This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and n otify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 12 Date: Wed, 04 Jun 2008 09:52:54 +0200 From: Ale? Kladnik Subject: [Histonet] Re: dome lid coplin jars To: histonet@lists.utsouthwestern.edu Message-ID: <484649D6.6080807@bf.uni-lj.si> Content-Type: text/plain; charset=UTF-8; format=flowed Try Bel-Art, they used to sell dome shaped coplin jars, now they claim they have an improved design with large opening - they even show a gloved hand taking a slide in/out of the jar. http://www.belart.com/cat/lifescience/en/442081000.asp > This is a small thing, but it would make my Pathology People so happy > (and me too!!) if I could order some plastic coplin jars with the domed > lid. Has anyone received any recently and if so, which supplier did you > use and catalog number? > > I've tried Cardinal and Fisher with no luck. Also Electron Microscopy > Sciences. They send the flat lidded, plastic coplin jar that is too > deep. > > The problem with the flat lidded, plastic coplin jars we have received > is that we can't pull slides out with just our gloved fingers, but must > instead use forceps, because they are so deep. The catalogs show a > picture of a domed lid, but they send you the flat lidded coplin jar. > > Any suggestions? > > Thanks, > Sandy -- Ale?? Kladnik Univerza v Ljubljani, Biotehni??ka fakulteta, Oddelek za biologijo Ve? na pot 111, SI-1000 Ljubljana, Slovenija tel: +386 1 4233388, fax: +386 1 2573390 url: http://botanika.biologija.org/kdosmo/aleskl.php url: http://web.bf.uni-lj.si/bi/mikroskopija/ ------------------------------ Message: 13 Date: Wed, 4 Jun 2008 09:46:12 +0100 From: "Jim Reilly" Subject: [Histonet] Re: Toluidine Blue and H&E To: Message-ID: <82B54C1E04D94D489448735E6CBDE4606DC2B5@exchange-be5.centre.ad.gla.ac.uk> Content-Type: text/plain; charset="US-ASCII" Hello I Tried Toluidine Blue / Eosin on decalcified mouse footpads : 2 minutes Tol Blue 1 minute Eosin rapid dehydration in 95% alcohol x1 100% x2 (10 dips each) clear in xylene and mount in DPX. This seemed to work OK. Jim Reilly Glasgow Biomedical Research Centre ------------------------------ Message: 14 Date: Wed, 4 Jun 2008 11:39:23 +0100 From: "Alan Bright" Subject: RE: [Histonet] Alternative to Histobath To: "Melissa Gonzalez" , Message-ID: Content-Type: text/plain; charset="iso-8859-1" Dear Melissa, Yes, we manufacture the Clini-RF Rapid Freezer which goes down to -80 degs. (See our Web site) C. We also fit as an option a Quick Freezer into our OTF5000 Cryostat. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype: dazzle0 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Melissa Gonzalez Sent: 03 June 2008 19:31 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Alternative to Histobath Anyone have any recommendations on tissue freezing units? Thanks! Melissa Melissa A. Gonz?lez Edick R&D, Cel l Genesys, Inc 500 Forbes Blvd South San Francisco, CA 94080 ph (650) 266-3168 fx (650) 266-3080 www.cellgenesys.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Wed, 4 Jun 2008 12:33:23 +0100 From: "Edwards, R.E." Subject: [Histonet] GMA immunos To: "histonet@lists.utsouthwestern.edu" Message-ID: <7722595275A4DD4FA225B92CDBF174A172A577C87E@EXC-MBX3.cfs.le.ac.uk> Content-Type: text/plain; charset="us-ascii" After a lifetime of immunostaining paraffin wax sections, in my new job I am having to deal with GMA sections. Two questions, firstly, does one have to float out the sections for a minimum of 90 seconds on 0.5% ammonium hydroxide before mounting on a slide;( with the ammonaical water apparently acting as some sort of antigen retrieval step), and immunohistochemistry on GMA sections does not work if one uses PBS as opposed to TBS. Any help/hints gratefully received. Cheers Richard Edwards University of Leicester. U.K. ------------------------------ Message: 16 Date: Wed, 04 Jun 2008 12:06:16 +0000 From: anitathorn@comcast.net Subject: RE: [Histonet] Creative Waste Solutions To: "Laurie Colbert" , "Angela Bitting" , Message-ID: <060420081206.14063.4846853800058ACF000036EF2200750330029D01089B0E9B07020E@c omcast.net> I work in a small volume lab and find that both the formalin and alcohol recycling systems are perfect for our needs. Easy to use and very economical. -------------- Original message ---------------------- From: "Laurie Colbert" > Angie, > > We use their formalin recycler, and we love it. It is very easy to use. > > Laurie Colbert > Huntington Hospital > Pasadena, CA > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela > Bitting > Sent: Monday, June 02, 2008 4:24 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Creative Waste Solutions > > Would users of recycling products from this company share feedback with > me? > > Thanks, > Angie > > Angela Bitting, HT(ASCP) > Technical Specialist, Histology > Geisinger Medical Center > 100 N Academy Ave. MC 23-00 > Danville, PA 17822 > phone 570-214-9634 > fax 570-271-5916 > > No trees were hurt in the sending of this email > However many electrons were severly inconvienienced! > > > > > IMPORTANT WARNING: The information in this message (and the documents > attached to it, if any) is confidential and may be legally privileged. > It is intended solely for the addressee. Access to this message by > anyone else is unauthorized. If you are not the intended recipient, any > disclosure, copying, distribution or any action taken, or omitted to be > taken, in reliance on it is prohibited and may be unlawful. If you have > received this message in error, please delete all electronic copies of > this message (and the documents attached to it, if any), destroy any > hard copies you may have created and notify me immediately by replying > to this email. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Wed, 4 Jun 2008 08:09:56 -0400 From: "Tom McNemar" Subject: RE: [Histonet] ML T working histology? To: , Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F57A@lmhsmail.lmhealth.org> Content-Type: text/plain; charset="iso-8859-1" I've had 3 or 4 MLT's over the years. They were trained to do every thing the HT's do. They were hired with the stipulation that they got their HT certification as well as having their MLT certification but only because it was a requirement for all of us. They have worked out well. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jennie Jenkins Sent: Wednesday, May 28, 2008 1:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MLT working histology? Can an MLT work in histology? She would be embedding, cutting, staining, cover slipping, labeling, filing, requisitioning and setting up the gross. If possible we could have her trained to gross in anything that would not require "cutting", like GI biopsies. Jennie Jenkins, PA(ASCP) Southside Community Hospital 800 Oak Street Farmville, Virginia 23901 ================================================== This e-mail message (and attachments) may contain information that is confidential to Southside Community Hospital. If you are not the intended recipient you cannot use, distribute or copy the message or attachments. In such a case, please notify the sender by return e-mail immediately and erase all copies of the message and attachments. Opinions, conclusions and other information in this message and attachments that do not relate to the official business of Southside Community Hospital are neither given nor endorsed by it. ================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Wed, 4 Jun 2008 05:12:24 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] xylene grades To: Merced Leiker , histonet@lists.utsouthwestern.edu Message-ID: <472664.60493.qm@web65713.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 The xylene "grade" (if such a thing exists!) is of a lesser concern than assuring a complete dewaxing of the section. You have to completely eliminate all the paraffin. Using distilled water to later immerse the sections in a buffer (that contains salts) is of no relevance, because you are going to "salinize" the sections any way. I think that your problem is with your Ab supplier, or the lot. CD117 is a "tricky" one. I stopped having problems when I switched to DAKO Mo Ab and HIER at pH6 Ren? J. Merced Leiker wrote: Would the grade of xylenes used for deparaffinizing make a difference for immunostaining of certain (picky) epitopes? We are using Certified A.C.S. xylenes. What about rinsing in tap water vs. distilled water during rehydration? The reason I'm asking is that we are having issues staining for c-kit (KIT, CD117) on FFPE tissue. We've tried all different antigen retrievals, including no retrieval, and still aren't seeing any stain. Any help would be greatly appreciated. Thanks! Merced M Leiker Research Technician II 354 BRB (Lee Lab) / 140 Farber Hall (mail) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Wed, 4 Jun 2008 08:20:19 - 0400 From: "Houston, Ronald" Subject: RE: [Histonet] MLT working histology? To: "Tom McNemar" , , Message-ID: <979FF5962E234F45B06CF0DB7C1AABB20FEC203C@chi2k3ms01.columbuschildrens.net> Content-Type: text/plain; charset="us-ascii" Why couldn't she? I have an MLT (taking her certification this year) and she is far superior to many of the "old timers" who claim to be histotechs but have absolutely no theoretical knowledge and are thus hopeless when it comes to troubleshooting. This profession needs to take the bull by the horns and change the outlook of histotechnology - it is a biomedical scientific profession and the training and examinations should reflect this. It is already lagging far behind MT's. The HT examination is a joke, and the QIHC certification is not much better. Now let the flaming begin!!!!!!!!!!! Ronnie Houston, MS, HT(ASCP)QIHC, AIBMS Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 Ronald.Houston@NationwideChildrens.org www.NationwideChildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Wednesday, June 04, 2008 8:10 AM To: jjenkins@sch-farmville.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] MLT working histology? I've had 3 or 4 MLT's over the years. They were trained to do every thing the HT's do. They were hired with the stipulation that they got their HT certification as well as having their MLT certification but only because it was a requirement for all of us. They have worked out well. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jennie Jenkins Sent: Wednesday, May 28, 2008 1:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MLT working histology? Can an MLT work in histology? She would be embedding, cutting, staining, cover slipping, labeling, filing, requisitioning and setting up the gross. If possible we could have her trained to gross in anything that would not require "cutting", like GI biopsies. Jennie Jenkins, PA(ASCP) Southside Community Hospital 800 Oak Street Farmville, Virginia 23901 ================================================== This e-mail message (and attachments) may contain information that is confidential to Southside Community Hospital. If you are not the intended recipient you cannot use, distribute or copy the message or attachments. In such a case, please notify the sender by return e-mail immediately and erase all copies of the message and attachments. Opinions, conclusions and other information in this message and attachments that do not relate to the official business of Southside Community Hospital are neither given nor endorsed by it. ================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ------------------------------ Message: 20 Date: Wed, 4 Jun 2008 08:48:41 -0400 From: "Pam Barker" Subject: [Histonet] RELIA Special JOB Alert To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Histonetters! I had one more position come in that I am pretty excited about. I have a client in the Philadelphia area in need of a senior histotechnologist. They are looking for someone who is ASCP certified with at least 5 years of experience. My client offers excellent compensation, benefits and a great place to work. Dermatopathology experience is a plus. If you are interested please contact me. I can be reached at relia1@earthlink.net or toll free at 866-607-3542. If you know someone who might be interested please feel free to pass the information along to them as well. Incidentally if you happen to know an ASCP certified med tech with microbiology experience I have a full time permanent 1st shift position in Cincinnati. Thanks and have a great day!! - Pam Thank You! Pam M. Barker President Relia 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll Free: (866)607-3542 e-mail: relia1@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia ------------------------------ Message: 21 Date: Wed, 4 Jun 2008 13:55:24 +0100 From: "Ian Montgomery" Subject: [Histonet] ATPase. To: Message-ID: Content-Type: text/plain; charset="us-ascii" About to trial two techniques for the demonstration of ATPase in human skeletal muscle. A Metachromatic Dye-ATPase Method for the Simultaneous Identification of Skeletal Muscle Fiber Types I, IIA, IIB and IIC. Ogilvie,R.W. & Feeback,D.L. 1990. Stain Technol. 65. 231-242 Inhibition Reactivation Myofibrillar ATPase Technique for Demonstration of Three Fiber Types in a Single Cryostat Section. Horak,V. & Matolin,S. 1990 Stain Technol. 65. 85-90 Has anyone tried these techniques and do you have any comments on their efficacy? They both appear straightforward with the papers giving details of the method. But like everything there is always the wee wrinkle that's omitted that makes the difference. So, why re-invent the wheel and give myself even more grey hair when someone might have the answer. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. ------------------------------ Message: 22 Date: Wed, 4 Jun 2008 08:59:01 -0400 From: "Whitaker, Bonnie" Subject: RE: [Histonet] MLT working histology? To: histonet@lists.utsouthwestern.edu Message-ID: <3CE20ED86C4A114EBDF3BCE8DEFD8F602624BF@msxc06.OSUMC.EDU> Content-Type: text/plain; charset=us-ascii Hi Everyone, I wanted to chime in on this one.... I think that there are people with just about any background who will make good histotechs, and people with just about any background who will make poor histotechs. It is very important for techs to understand the theory behind the procedures that they are doing, and this can be accomplished in many ways. It generally will be better if someone has enough of a science background to already understand some of the basic principles involved in histotechnology, but there will always be the exceptional person (on both sides of the curve). Rather than focusing on whether a person is an MLT, an MT, has a biology degree, etc., it would probably be better to focus on what kind of drive that person seems to have, and whether or not they are looking for a "job" or for a "career", and make it clear that they are expected to obtain their registry and undergo regular competency assessments that should do more than "pay lipservice" to the regulations. (Of course I'm speaking for clinical labs that are CLIA and/or CAP.) Bonnie Whitaker, MT(HEW), HT(ASCP)QIHC Clinical Histology Manager Ohio State University Medical Center N308B Doan Hall 410 W. 10th Ave. Columbus, OH 43210 614.293.5048 Bonnie.Whitaker@osumc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Wednesday, June 04, 2008 8:20 AM To: Tom McNemar; jjenkins@sch-farmville.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] MLT working histology? Why couldn't she? I have an MLT (taking her certification this year) and she is far superior to many of the "old timers" who claim to be histotechs but have absolutely no theoretical knowledge and are thus hopeless when it comes to troubleshooting. This profession needs to take the bull by the horns and change the outlook of histotechnology - it is a biomedical scientific profession and the training and examinations should reflect this. It is already lagging far behind MT's. The HT examination is a joke, and the QIHC certification is not much better. Now let the flaming begin!!!!!!!!!!! Ronnie Houston, MS, HT(ASCP)QIHC, AIBMS Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 Ronald.Houston@NationwideChildrens.org www.NationwideChildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Wednesday, June 04, 2008 8:10 AM To: jjenkins@sch-farmville.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] MLT working histology? I've had 3 or 4 MLT's over the years. They were trained to do every thing the HT's do. They were hired with the stipulation that they got their HT certification as well as having their MLT certification but only because it was a requirement for all of us. They have worked out well. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jennie Jenkins Sent: Wednesday, May 28, 2008 1:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MLT working histology? Can an MLT work in histology? She would be embedding, cutting, staining, cover slipping, labeling, filing, requisitioning and setting up the gross. If possible we could have her trained to gross in anything that would not require "cutting", like GI biopsies. Jennie Jenkins, PA(ASCP) Southside Community Hospital 800 Oak Street Farmville, Virginia 23901 ================================================== This e-mail message (and attachments) may contain information that is confidential to Southside Community Hospital. If you are not the intended recipient you cannot use, distribute or copy the message or attachments. In such a case, please notify the sender by return e-mail immediately and erase all copies of the message and attachments. Opinions, conclusions and other information in this message and attachments that do not relate to the official business of Southside Community Hospital are neither given nor endorsed by it. ================================================== _______________________________________________ Histonet mailin g list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 55, Issue 4 *************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jerry <@t> ralambusa.com Wed Jun 4 11:33:29 2008 From: Jerry <@t> ralambusa.com (Jerry Helisek) Date: Wed Jun 4 11:33:37 2008 Subject: [Histonet] RE: Special Stains Kit Message-ID: <3855F92002259948A66A8CA2D16E3A4F0B546D@server.ralambusa.com> Tracey, I do not know for sure, but have you tried American Mastertech in Northern California? ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ Message: 7 Date: Wed, 4 Jun 2008 08:52:25 -0600 From: Subject: [Histonet] Special Stains Kit To: Message-ID: <695BA3B5A811274182C175122F8663C02959FF@mail1.calgary.com> Content-Type: text/plain; charset="iso-8859-1" Hi, Does anyone know of a supplier for a Chloroacetate esterase kit other than Sigma? Thanks Tracey CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mai or telephone and destroy the entire transmission and any copies produced. Thank you. From awatanabe <@t> tgen.org Wed Jun 4 12:29:40 2008 From: awatanabe <@t> tgen.org (Aprill Watanabe) Date: Wed Jun 4 12:29:47 2008 Subject: [Histonet] RE:Disposal of tissue blocks Message-ID: Most places use an incinerator. But on the other hand I have another suggestion. I work in research and we are always looking for ways to build up our tissue block resources. We make tissue microarrays and do tons of IHC on the TMAs. I would love to have another site I could get some blocks from. We routinely set up IRB approved projects to obtain a certain number of blocks with limited path report information which stays in line with HIPPA guidelines. If this might sound like something your facility would be interested in please contact me in private. Aprill Watanabe, B.S. Research Associate Integrated Cancer Genomics Division Tissue Microarray Center (TMA) Translational Genomics Research Institute (TGen) main: 602-343-8822 Fax: 602-343-8840 awatanabe@tgen.org www.tgen.org From neelyk <@t> shands.ufl.edu Wed Jun 4 13:14:01 2008 From: neelyk <@t> shands.ufl.edu (Kendall Neely) Date: Wed Jun 4 13:14:27 2008 Subject: [Histonet] Free Histo-Loc cassettes! Message-ID: <4846A328.3024.0059.0@shands.ufl.edu> Hi Histonetters: We have cases and cases of Richard Allen Histo-Loc (mesh screen) cassettes in various colors. We cannot use them and are offering them FREE to anyone interested. You just pay shipping! Email me at neelyk@shands.ufl.edu if interested. Thanks! Kendall A. Neely Histology Technical Specialist Shands Rocky Point Laboratories (352) 265-0111, x72113 From Jackie.O'Connor <@t> abbott.com Wed Jun 4 13:36:11 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed Jun 4 13:36:31 2008 Subject: [Histonet] RE:Disposal of tissue blocks In-Reply-To: Message-ID: HIPPA might have a problem with that. That's why we can't even get extra tonsils for controls anymore. HIPPA HIPPa Hooray. I Jackie Aprill Watanabe Sent by: histonet-bounces@lists.utsouthwestern.edu 06/04/2008 12:29 PM To cc Subject [Histonet] RE:Disposal of tissue blocks Most places use an incinerator. But on the other hand I have another suggestion. I work in research and we are always looking for ways to build up our tissue block resources. We make tissue microarrays and do tons of IHC on the TMAs. I would love to have another site I could get some blocks from. We routinely set up IRB approved projects to obtain a certain number of blocks with limited path report information which stays in line with HIPPA guidelines. If this might sound like something your facility would be interested in please contact me in private. Aprill Watanabe, B.S. Research Associate Integrated Cancer Genomics Division Tissue Microarray Center (TMA) Translational Genomics Research Institute (TGen) main: 602-343-8822 Fax: 602-343-8840 awatanabe@tgen.org www.tgen.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jwatson <@t> gnf.org Wed Jun 4 13:50:44 2008 From: jwatson <@t> gnf.org (James Watson) Date: Wed Jun 4 13:50:49 2008 Subject: [Histonet] RE:Disposal of tissue blocks In-Reply-To: References: Message-ID: If a hospital donates tissue blocks with no patient information on the blocks and with no path report to a research facility is that within HIPPA regulations? Jamie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Wednesday, June 04, 2008 11:36 AM To: Aprill Watanabe Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] RE:Disposal of tissue blocks HIPPA might have a problem with that. That's why we can't even get extra tonsils for controls anymore. HIPPA HIPPa Hooray. I Jackie Aprill Watanabe Sent by: histonet-bounces@lists.utsouthwestern.edu 06/04/2008 12:29 PM To cc Subject [Histonet] RE:Disposal of tissue blocks Most places use an incinerator. But on the other hand I have another suggestion. I work in research and we are always looking for ways to build up our tissue block resources. We make tissue microarrays and do tons of IHC on the TMAs. I would love to have another site I could get some blocks from. We routinely set up IRB approved projects to obtain a certain number of blocks with limited path report information which stays in line with HIPPA guidelines. If this might sound like something your facility would be interested in please contact me in private. Aprill Watanabe, B.S. Research Associate Integrated Cancer Genomics Division Tissue Microarray Center (TMA) Translational Genomics Research Institute (TGen) main: 602-343-8822 Fax: 602-343-8840 awatanabe@tgen.org www.tgen.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ploykasek <@t> phenopath.com Wed Jun 4 13:50:49 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Wed Jun 4 13:50:59 2008 Subject: [Histonet] RE:Disposal of tissue blocks In-Reply-To: Message-ID: At my lab we have made some TMAs and usually don't have a problem with HIPPA with the IRB approval. The IRB approval is not an easy process, and the blocks & tumor information come with no patient ID info. I would be interested to hear of others experience with IRB, HIPPA, etc... I do agree with Jackie - HIPPA HIPPA Hooray. It's a headache. Patti > HIPPA might have a problem with that. That's why we can't even get extra > tonsils for controls anymore. HIPPA HIPPa Hooray. I > > Jackie > > > > Aprill Watanabe > Sent by: histonet-bounces@lists.utsouthwestern.edu > 06/04/2008 12:29 PM > > To > > cc > > Subject > [Histonet] RE:Disposal of tissue blocks > > > > > > > Most places use an incinerator. But on the other hand I have another > suggestion. I work in research and we are always looking for ways to > build > up our tissue block resources. We make tissue microarrays and do tons of > IHC on the TMAs. I would love to have another site I could get some > blocks > from. We routinely set up IRB approved projects to obtain a certain > number > of blocks with limited path report information which stays in line with > HIPPA guidelines. If this might sound like something your facility would > be > interested in please contact me in private. > > Aprill Watanabe, B.S. > Research Associate > Integrated Cancer Genomics Division > Tissue Microarray Center (TMA) > Translational Genomics Research Institute (TGen) > main: 602-343-8822 > Fax: 602-343-8840 > awatanabe@tgen.org > www.tgen.org > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From neelyk <@t> shands.ufl.edu Wed Jun 4 13:55:20 2008 From: neelyk <@t> shands.ufl.edu (Kendall Neely) Date: Wed Jun 4 13:55:55 2008 Subject: [Histonet] Histo-Loc cassettes are taken Message-ID: <4846ACD8.3024.0059.0@shands.ufl.edu> Hi everyone, I appreciate your rapid response! I have found a taker. Thanks! Kendall A. Neely Histology Technical Specialist Shands Rocky Point Laboratories (352) 265-0111, x72113 From rjbuesa <@t> yahoo.com Wed Jun 4 14:48:15 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 4 14:48:22 2008 Subject: [Histonet] HT practical In-Reply-To: Message-ID: <254966.88193.qm@web65710.mail.ac4.yahoo.com> Yes, the practical portion has been discontinued! Ren? J. "Martin, Erin" wrote: Hi everyone, I have two techs who are getting ready to apply for the HT exam but I can't find any info about the practical portion of the exam. Was it discontinued? Or am I not looking in the right place on the ASCP BOR page? Thanks Erin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcline <@t> wchsys.org Wed Jun 4 15:43:49 2008 From: jcline <@t> wchsys.org (Joyce Cline) Date: Wed Jun 4 15:43:53 2008 Subject: [Histonet] RE: block disposal Message-ID: I have the same company that picks up our Sub/Alcohol waste, pack up our blocks and incinerate them. ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From rema.oliver <@t> unsw.edu.au Wed Jun 4 16:39:45 2008 From: rema.oliver <@t> unsw.edu.au (Rema) Date: Wed Jun 4 16:48:35 2008 Subject: [Histonet] Staining for Hyaluronic acid Message-ID: <48470BA1.40406@unsw.edu.au> - Hi, I am trying to stain some rabbit skin to detect Hyaluronic acid but have found that my staining is very weak even though I have left the samples in 1% Alcian blue (pH 2.5) for 30 mins which is what standard procedures from Histo technique books say to do. I am also counterstaining with neutral red. Does anyone have a procedure that knows works or could suggest how I can increase the staining intensity? Many thanks, Rema -- Rema Oliver PhD Research Fellow Surgical & Orthopaedic Research Laboratories Level 1, Clinical Sciences Bldg Prince of Wales Hospital Ph: +61 2 9382 2654 Fax: +61 2 9382 2660 Mobile: 0419 605 034 From PMonfils <@t> Lifespan.org Wed Jun 4 17:36:38 2008 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Jun 4 17:36:43 2008 Subject: [Histonet] Staining for Hyaluronic acid In-Reply-To: <48470BA1.40406@unsw.edu.au> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273D8D@LSRIEXCH1.lsmaster.lifespan.org> I just finished the same technique 10 minutes ago! Alcian blue for hyaluronic acid, with and without hyaluronidase. Hyaluronic acid is seldom present in tissues in high concentration. When the entity you are staining is sparse, your staining is going to be light. There is really no way around that. I use umbilical cord as a control. Hyaluronic acid is present in this tissue in higher concentration than in most other tissues. You can also try to increase your staining intensity by cutting your sections a little thicker. From jnocito <@t> satx.rr.com Wed Jun 4 17:45:53 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Jun 4 17:45:43 2008 Subject: [Histonet] HT practical References: Message-ID: <005b01c8c694$bf546de0$0302a8c0@yourxhtr8hvc4p> Erin, the practical portion was discontinued a couple of years ago. The exam is computer only. I was told that it was getting too expensive to fly people to Chicago to review the slides. I understand that the questions go into depth and critical thinking and problem solving is a majority of the exam now. JTT ----- Original Message ----- From: "Martin, Erin" To: "histonet" Sent: Wednesday, June 04, 2008 10:59 AM Subject: [Histonet] HT practical Hi everyone, I have two techs who are getting ready to apply for the HT exam but I can't find any info about the practical portion of the exam. Was it discontinued? Or am I not looking in the right place on the ASCP BOR page? Thanks Erin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Wed Jun 4 17:59:44 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Jun 4 18:02:08 2008 Subject: [Histonet] Staining for Hyaluronic acid In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E273D8D@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: Try one of the colloidal iron methods. This seems to detect smaller amounts of carboxylated mucins then the alcian blue methods. This is why several dermatopathologists prefer it for skin biopsies. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Thursday, 5 June 2008 8:37 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Staining for Hyaluronic acid I just finished the same technique 10 minutes ago! Alcian blue for hyaluronic acid, with and without hyaluronidase. Hyaluronic acid is seldom present in tissues in high concentration. When the entity you are staining is sparse, your staining is going to be light. There is really no way around that. I use umbilical cord as a control. Hyaluronic acid is present in this tissue in higher concentration than in most other tissues. You can also try to increase your staining intensity by cutting your sections a little thicker. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From Norm.Burnham <@t> propath.com Wed Jun 4 18:02:19 2008 From: Norm.Burnham <@t> propath.com (Norm Burnham) Date: Wed Jun 4 18:02:30 2008 Subject: [Histonet] Histology and IHC Opportunities in Dallas, TX Message-ID: <332B4C097301BD42BEC7AA93D36CE5F13FFF9C@mail.propathlab.com> ProPath(r) is a progressive, CAP accredited, high-volume pathology practice located in Dallas, Texas. We are seeking candidates for the following positions: HISTOLOGY MANAGER Responsibilities include staffing, management, planning, coordination and evaluation of all technical and operational activities of the Histology laboratory. Ensure that department is operating efficiently and producing the highest quality of work. Requirements include HT/HTL (ASCP) certification, a minimum of 8 years histology experience plus a minimum of 5 years supervisory experience. BS degree in Biological/Physical Science preferred, Associate degree required. Must be available to work nights as needed. IHC SUPERVISOR Responsibilities include recruiting, hiring, training and scheduling staff; supervising IHC laboratory operation. Ensure quality of slides and department goals are met; maintaining preparedness for all accrediting/regulatory agency inspections, and general troubleshooting. Requirements include HT/HTL (ASCP) certification, a minimum of 5 years histology experience plus a minimum of 1 - 2 years supervisory experience. BS or Associate degree in Biological/Physical Science preferred. Must be available to work nights as needed. HISTOTECHNICIAN Responsibilities include embedding tissue specimens, microtomy of paraffin-embedded tissue, operations of automated stainer and cover slipper, equipment maintenance, and record retention. HT/ HTL (ASCP) registered or eligible preferred. Experience preferred. Hours are 1:00 a.m. to 9:30 a.m. M-F (Eligible for shift differential) IHC TECH Responsibilities include slide preparation, staining, antibody titer preparation, equipment maintenance, supply and reagent inventory maintenance, and record retentions. HT/ HTL/ QIHC (ASCP) registered or eligible preferred. Experience preferred. Hours are 4:00 p.m. to 12:30 a.m. M-F (Eligible for shift differential) ProPath utilizes leading technology and is a quality oriented pathology practice. Benefits include relocation assistance, medical, dental, short and long-term disability insurance, a matched 401(k) plan and more! For consideration send resume to Human Resources, ProPath , 8267 Elmbrook, Suite 100, Dallas, TX 75247. FAX: 214/237-1825. Job-Line 214/237-1775. Email address. jobs@propath.com Website www.propath.com. EOE. Kitty Maxey Human Resources Director ProPath - The Leader in Pathology Services 8267 Elmbrook Dr., Suite 100 Dallas, TX 75247 214/237-1608 (direct) 214/237-1808 (fax) 1-800-258-1253, ext. 1608 (toll-free) 214/237-1775 (Job Line) To learn more about ProPath, please visit http://www.ProPath.com ______________________________________________________________________________ This e-mail may contain confidential or privileged information. If you think you have received this e-mail in error, please advise the sender by reply e-mail and then delete this e-mail immediately. From dgaupp <@t> tulane.edu Wed Jun 4 18:16:02 2008 From: dgaupp <@t> tulane.edu (Gaupp, Dina D ) Date: Wed Jun 4 18:16:06 2008 Subject: [Histonet] mouse heart chatter upon sectioning... Message-ID: <447056A67472B241A330A525B4AF716720621B@EX02.ad.tulane.edu> Whenever I process (whole)mouse hearts, the tissue chatters upon sectioning. The tissue is usually fixed overnight in 10%nbf at 4C before processed. I tried different processing times. The last time I processed hearts, I processed them for 5 hours total no vaccum. Should I decrease the processing time even lower than that? I am afraid of underprocessing. I have to section the entire heart from top to bottom, so I really need some advice. (open system) Processor Set Up: 1 - fix (if tissue fixed overnight, skip #1 & #2 stations) 2 - fix 3 - 70% 4 - 80% 5 & 6 - 95% 7 & 8 - 100% 9 & 10- Xylene Sub. - Clear-rite 11 & 12-Paraffin stations Thanks, Dina dgaupp@tulane.edu From AnthonyH <@t> chw.edu.au Wed Jun 4 18:23:13 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Jun 4 18:23:19 2008 Subject: [Histonet] mouse heart chatter upon sectioning... In-Reply-To: <447056A67472B241A330A525B4AF716720621B@EX02.ad.tulane.edu> Message-ID: Several queries, Why fix in the fridge? Do you want to slow down the fixation or introduce ice crystal artifact? If the tissue is shattering, one of the problems might be the fact that the tissue is fixed in ethanol reather than formalin. What are the processing times & temperatures. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gaupp, Dina D Sent: Thursday, 5 June 2008 9:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] mouse heart chatter upon sectioning... Whenever I process (whole)mouse hearts, the tissue chatters upon sectioning. The tissue is usually fixed overnight in 10%nbf at 4C before processed. I tried different processing times. The last time I processed hearts, I processed them for 5 hours total no vaccum. Should I decrease the processing time even lower than that? I am afraid of underprocessing. I have to section the entire heart from top to bottom, so I really need some advice. (open system) Processor Set Up: 1 - fix (if tissue fixed overnight, skip #1 & #2 stations) 2 - fix 3 - 70% 4 - 80% 5 & 6 - 95% 7 & 8 - 100% 9 & 10- Xylene Sub. - Clear-rite 11 & 12-Paraffin stations Thanks, Dina dgaupp@tulane.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From freckles9660 <@t> yahoo.com Wed Jun 4 18:37:47 2008 From: freckles9660 <@t> yahoo.com (Karla Arrington) Date: Wed Jun 4 18:37:49 2008 Subject: [Histonet] Legality of work Message-ID: <163789.93086.qm@web32506.mail.mud.yahoo.com> Histo's: ? I have roughly 2 dilemmas.? The first is of a legal matter.? The Pathologist for a week has had his daughter (15 years old), helping me out in the histology lab.? He wants her to get training from me, then do the on-line HT program.? She has handled chemicals and reagents.? I am very uncomfortable with this.? I have called the Child Labor Laws department for our state and it is illegal for a 15 year to be in a laboratory. Needless to say working with blood borne pathogens.? My most concern is the following.? As her "teacher", can I or other co-workers can be held accountable if this is illegal and is found out??This pathologist mind you is the owner of the business.? I am afraid if I say something, I will get fired. Where do I go from here or if there is someone who has a similar circumstance happen to them. The other scenario is that this same Pathologist has called a tech a slandering name, twice.? There is no "upper management" to go to since he is the owner of the business. I was wondering if this is considered harassment and can this be used to?nullify a contract signed by both parties for employment. ? Concerned, freckles9660@yahoo.com From AnthonyH <@t> chw.edu.au Wed Jun 4 19:40:41 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Jun 4 19:40:56 2008 Subject: [Histonet] mouse heart chatter upon sectioning... In-Reply-To: <447056A67472B241A330A525B4AF716720621C@EX02.ad.tulane.edu> Message-ID: Ice crystals can form at"4oC" - Fridge temperatures will vary, often colder than the stated temp, often warmer. I did not suggest that ice crystal formation would cause shatter but it will effect morphology (see Henwood, A., (2005) "Effect of Slide Drying at 80?C on Immunohistochemistry" J Histotechnol 28(1):45-46.). Some researches (and I say SOME not all), often with limited understanding of the processes of histotechnology, will sprout expertise and knowledge that is based on flimsy evidence. Fixing in formalin seems to be one of these. Someone decided in their wisdom to fix at 4oC. Why?, Based on what? So others thought it sounded good to do it this way and problems have occurred ever since. Preventing over-fixation in formalin will invariably cause over-fixation in ethanol. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: Gaupp, Dina D [mailto:dgaupp@tulane.edu] Sent: Thursday, 5 June 2008 9:38 AM To: Tony Henwood Subject: RE: [Histonet] mouse heart chatter upon sectioning... Fix @4C to prevent overfixation. I didn't think ice crystals could form @4C. Process temps are all performed at room temp. except of course, paraffin tremp is 57C. Process times vary according to size & complexity of tissue. The last process time for mouse hearts was total of 5hrs. But it still didn't work. The tissue still chattered. I had to soak the block in ice cold water to soften the tissue. What schedule would you recommend processing mouse hearts? Alot of the principle investigators like to leave their tissues at 4C in fix. Does this form ice crystals? Only thing is every other tissue besides hearts cut perfect. So I wouldn't think it would be ice crystals making it chatter. Dina From: Tony Henwood [mailto:AnthonyH@chw.edu.au] Sent: Wed 6/4/2008 6:23 PM To: Gaupp, Dina D ; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] mouse heart chatter upon sectioning... Several queries, Why fix in the fridge? Do you want to slow down the fixation or introduce ice crystal artifact? If the tissue is shattering, one of the problems might be the fact that the tissue is fixed in ethanol reather than formalin. What are the processing times & temperatures. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gaupp, Dina D Sent: Thursday, 5 June 2008 9:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] mouse heart chatter upon sectioning... Whenever I process (whole)mouse hearts, the tissue chatters upon sectioning. The tissue is usually fixed overnight in 10%nbf at 4C before processed. I tried different processing times. The last time I processed hearts, I processed them for 5 hours total no vaccum. Should I decrease the processing time even lower than that? I am afraid of underprocessing. I have to section the entire heart from top to bottom, so I really need some advice. (open system) Processor Set Up: 1 - fix (if tissue fixed overnight, skip #1 & #2 stations) 2 - fix 3 - 70% 4 - 80% 5 & 6 - 95% 7 & 8 - 100% 9 & 10- Xylene Sub. - Clear-rite 11 & 12-Paraffin stations Thanks, Dina dgaupp@tulane.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From laurie.reilly <@t> jcu.edu.au Wed Jun 4 19:46:51 2008 From: laurie.reilly <@t> jcu.edu.au (Laurie Reilly) Date: Wed Jun 4 19:48:59 2008 Subject: [Histonet] mouse heart chatter upon sectioning... In-Reply-To: <447056A67472B241A330A525B4AF716720621B@EX02.ad.tulane.edu> References: <447056A67472B241A330A525B4AF716720621B@EX02.ad.tulane.edu> Message-ID: <000b01c8c6a5$a4c3ad90$5255db89@health.ad.jcu.edu.au> Dina, Rodent tissues are notorious for becoming brittle, even with the gentlest processing. Try rehydrating the tissues a little in the paraffin block. After you trim the face of the paraffin block, float it face down on your waterbath for 30 seconds. Dry the face of the block, put it back on ice to cool, and then try sectioning. You should be able to get 5 or 6 good sections before it starts to shatter again. This is a fact of Histo life that I have lived with for 30 years and I would love to hear a scientific reason why some tissues, particularly from rodents, small birds and reptiles give this trouble. Can anyone help with an explanation? Regards, Laurie. Mr. Laurie REILLY Histopathology School of Veterinary and Biomedical Sciences James Cook University Townsville Qld. 4811 Australia. Phone 07 4781 4468 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gaupp, Dina D Sent: Thursday, 5 June 2008 9:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] mouse heart chatter upon sectioning... Whenever I process (whole)mouse hearts, the tissue chatters upon sectioning. The tissue is usually fixed overnight in 10%nbf at 4C before processed. I tried different processing times. The last time I processed hearts, I processed them for 5 hours total no vaccum. Should I decrease the processing time even lower than that? I am afraid of underprocessing. I have to section the entire heart from top to bottom, so I really need some advice. (open system) Processor Set Up: 1 - fix (if tissue fixed overnight, skip #1 & #2 stations) 2 - fix 3 - 70% 4 - 80% 5 & 6 - 95% 7 & 8 - 100% 9 & 10- Xylene Sub. - Clear-rite 11 & 12-Paraffin stations Thanks, Dina dgaupp@tulane.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From suriana <@t> spdscientific.com.sg Wed Jun 4 20:37:33 2008 From: suriana <@t> spdscientific.com.sg (Suriana Bte Abdul Latiff) Date: Wed Jun 4 20:37:45 2008 Subject: [Histonet] Re: Alternative to Histobath In-Reply-To: Message-ID: <2EF74098586A7B44B45162438E0F3CD2728D5D@Lithium.LABGISTICS.COM> Maybe you would want to try out this? http://www.alphelys.com/site/us/pCT_CryoBain.htm Regards Sue -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Thursday, June 05, 2008 12:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Alternative to Histobath Replacement for the Histobath rapid freezing unit, apparently no longer in manufacture: Alan Bright, at Bright Instrument Co. in England, notes that >>we manufacture the Clini-RF Rapid Freezer which goes down to -80 degs. (See our Web site) C. We also fit as an option a Quick Freezer into our OTF5000 Cryostat.<< Histobath consisted of a container of liquid cooled to a low temperature by a freezer. The open pot was filled with acetone (or 2-methylbutane), and I thought it was an explosion hazard even at the very low temperature the liquid was held at. At brightinstrument.com I read that the liquid used in it was hexane, also a fire hazard in an open container. I understood that some people were using a hydrofluoroether in the Histobath. Can this be used in the Clini-RF unit? The information I had about this stuff was: 3M(tm) Novec(tm) Engineered Fluid HFE-7100 This product belongs to a class of fluorocarbons called "segregated hydrofluoroethers (HFE's)" According to various MSDS, HFE-7100 is methyl nonafluoroisobutyl ether C4F9-O-CH3 It melts and freezes at -135 C, so that it would remain liquid in the Histobath. (It would freeze solid in liquid nitrogen, however.) It boils at 60 C., and is listed as non-flammable. It cost around $230 a gallon a few years ago. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Malcolm.McCallum <@t> tamut.edu Wed Jun 4 21:10:09 2008 From: Malcolm.McCallum <@t> tamut.edu (Malcolm McCallum) Date: Wed Jun 4 21:10:58 2008 Subject: [Histonet] RE:Disposal of tissue blocks References: Message-ID: I save mine for the next birthday cake...... hehe! Just could not resist! Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-223-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html VISIT HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org A New Journal Published in Partnership with Partners in Amphibian and Reptile Conservation and the World Congress of Herpetology. Spring Teaching Schedule & Office Hours: Genetics: W 6:00 to 9:40pm Herpetology: TR 10:00-11:40am Histology: MW 1:00-2:40pm Seminar: T 2:30-3:30pm Office Hours: M: 3:30-5:00pm T: 11:40-1:00pm; 3:30-5:00pm W: 4:00-6:00pm "We live in a time when lemonade is made with artificial flavoring, and furnisher polish is made with fresh lemons." -Alfred E. Neuman -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Patti Loykasek Sent: Wed 6/4/2008 1:50 PM To: histonet Subject: Re: [Histonet] RE:Disposal of tissue blocks At my lab we have made some TMAs and usually don't have a problem with HIPPA with the IRB approval. The IRB approval is not an easy process, and the blocks & tumor information come with no patient ID info. I would be interested to hear of others experience with IRB, HIPPA, etc... I do agree with Jackie - HIPPA HIPPA Hooray. It's a headache. Patti > HIPPA might have a problem with that. That's why we can't even get extra > tonsils for controls anymore. HIPPA HIPPa Hooray. I > > Jackie > > > > Aprill Watanabe > Sent by: histonet-bounces@lists.utsouthwestern.edu > 06/04/2008 12:29 PM > > To > > cc > > Subject > [Histonet] RE:Disposal of tissue blocks > > > > > > > Most places use an incinerator. But on the other hand I have another > suggestion. I work in research and we are always looking for ways to > build > up our tissue block resources. We make tissue microarrays and do tons of > IHC on the TMAs. I would love to have another site I could get some > blocks > from. We routinely set up IRB approved projects to obtain a certain > number > of blocks with limited path report information which stays in line with > HIPPA guidelines. If this might sound like something your facility would > be > interested in please contact me in private. > > Aprill Watanabe, B.S. > Research Associate > Integrated Cancer Genomics Division > Tissue Microarray Center (TMA) > Translational Genomics Research Institute (TGen) > main: 602-343-8822 > Fax: 602-343-8840 > awatanabe@tgen.org > www.tgen.org > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Jun 5 07:37:37 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jun 5 07:37:47 2008 Subject: [Histonet] mouse heart chatter upon sectioning... In-Reply-To: <447056A67472B241A330A525B4AF716720621B@EX02.ad.tulane.edu> Message-ID: <310509.41644.qm@web65704.mail.ac4.yahoo.com> Mouse tissues are specially low in fat and prone to become brittle with too much ethanol or xylene. I think you should cut the dehydration times in half and use isopropanol (ISO) instead of ethanol. After the ISO place the hearts in a mixture of ISO and mineral oil (M) 5:1 for 1 hour at 50?C, another hour in a ISO:M mixture 2:1 at 50?C; another hour in pure M at 50?C, another hour in a 1:1 mixture of M and paraffin at 50?C, and complete your infiltration with paraffin as usual. This method has been a modification by Maxim Peshkov of my mineral oil method, Give it a try. It is now standard procedure in a veterinary lab in the UK. Ren? J. "Gaupp, Dina D " wrote: Whenever I process (whole)mouse hearts, the tissue chatters upon sectioning. The tissue is usually fixed overnight in 10%nbf at 4C before processed. I tried different processing times. The last time I processed hearts, I processed them for 5 hours total no vaccum. Should I decrease the processing time even lower than that? I am afraid of underprocessing. I have to section the entire heart from top to bottom, so I really need some advice. (open system) Processor Set Up: 1 - fix (if tissue fixed overnight, skip #1 & #2 stations) 2 - fix 3 - 70% 4 - 80% 5 & 6 - 95% 7 & 8 - 100% 9 & 10- Xylene Sub. - Clear-rite 11 & 12-Paraffin stations Thanks, Dina dgaupp@tulane.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Jun 5 07:50:58 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jun 5 07:51:02 2008 Subject: [Histonet] Legality of work In-Reply-To: <163789.93086.qm@web32506.mail.mud.yahoo.com> Message-ID: <466600.51945.qm@web65704.mail.ac4.yahoo.com> Here is how I see your problem: 1- you have already contacted the Child and Labor Dept. about this issue, so it is very likely (if they are doing their job) that the will "pay a visit" to the lab and talk with the pathologist. 2- if that is what is going to happen you are going to be in trouble. 3- as the "youngster's" trainer you are accomplice to the violation, because you know her age and keep training her. 4- if this pathologist is also calling names to others, he is an absolute jerk that thinks is owner, not only of the place, but of the world. 5- I don't think this is the right place to work so sooner or later you should try to find a new place to work. 6- if that is going to be how this whole issue is going to end, I think that you should do what is correct and tell him that you are not willing to participate in violating the laboral laws and stop training his daughter. You will sleep better and perhaps this fellow will realize that he is doing something wrong and that you are not willing to accept the situation. At least that is what I would do. Consult with some close friend or family member and present a formal complaint with Child Labor BEFORE doing #6. If he fires you because of this then you will have PROOF that you complained against him because of a law violation and will be able to claim unemployment, even if he says that you were fired for "work substandard performance", you will have proof that this is not the case, but revenge against you. Good luck! Ren? J. Karla Arrington wrote: Histo's: I have roughly 2 dilemmas. The first is of a legal matter. The Pathologist for a week has had his daughter (15 years old), helping me out in the histology lab. He wants her to get training from me, then do the on-line HT program. She has handled chemicals and reagents. I am very uncomfortable with this. I have called the Child Labor Laws department for our state and it is illegal for a 15 year to be in a laboratory. Needless to say working with blood borne pathogens. My most concern is the following. As her "teacher", can I or other co-workers can be held accountable if this is illegal and is found out? This pathologist mind you is the owner of the business. I am afraid if I say something, I will get fired. Where do I go from here or if there is someone who has a similar circumstance happen to them. The other scenario is that this same Pathologist has called a tech a slandering name, twice. There is no "upper management" to go to since he is the owner of the business. I was wondering if this is considered harassment and can this be used to nullify a contract signed by both parties for employment. Concerned, freckles9660@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Thu Jun 5 08:04:54 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Jun 5 08:05:10 2008 Subject: [Histonet] HT practical In-Reply-To: <005b01c8c694$bf546de0$0302a8c0@yourxhtr8hvc4p> Message-ID: <000101c8c70c$c27372a0$d00f7ca5@lurie.northwestern.edu> So why can't slides be sent to reviewers? I always thought the practical was good as it shows a tech can cut (which may be an issue) as well as know staining techniques.how can you troubleshoot micrometry issues if you in theory may or may not be cutting? When I first cam into the field, the first thing done in my interview was to sit me at a microtome and show I could cut.After I was hired I had 3 months to get the quality and quantity of techs with at least 10 years experience on me. Needless to say, I can cut and darn well at that! Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, June 04, 2008 5:46 PM To: Martin, Erin; histonet Subject: Re: [Histonet] HT practical Erin, the practical portion was discontinued a couple of years ago. The exam is computer only. I was told that it was getting too expensive to fly people to Chicago to review the slides. I understand that the questions go into depth and critical thinking and problem solving is a majority of the exam now. JTT ----- Original Message ----- From: "Martin, Erin" To: "histonet" Sent: Wednesday, June 04, 2008 10:59 AM Subject: [Histonet] HT practical Hi everyone, I have two techs who are getting ready to apply for the HT exam but I can't find any info about the practical portion of the exam. Was it discontinued? Or am I not looking in the right place on the ASCP BOR page? Thanks Erin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMcCormick <@t> schosp.org Thu Jun 5 08:29:08 2008 From: JMcCormick <@t> schosp.org (McCormick, James) Date: Thu Jun 5 08:29:19 2008 Subject: [Histonet] Legality of work In-Reply-To: <466600.51945.qm@web65704.mail.ac4.yahoo.com> References: <163789.93086.qm@web32506.mail.mud.yahoo.com> <466600.51945.qm@web65704.mail.ac4.yahoo.com> Message-ID: <95D447442469F44DB2A370783169A3E804DB2667@exch01.backup> >From an interested Pathologist: I think you are building a bomb to attack an mouse! When our son and daughter were 14 and 15 years old I was proud to have them VOLUNTEER (non-paid) service to the our laboratory. First to meet the fine people who worked in the department. Second to learn about "dad's work", and third to develop a sense of appreciation for our work in serving others. The children benefited by the social relationship and grew in responsibility by graduating from tasks of simple "go for's", observers, awarded responsibility. They were not permitted to prepare patient materials but they were, over a period of summer volunteer effort, taught to make a collection of their own slides and even to do a frozen section. The experience is a treasured memory and valued experience for the Pathologist and his family and for the bonding of employees who for that period were extended members of the family. Our son is now completing his residency in orthopaedic Surgery and our daughter is a junior officer in a New York company where she has employees reporting to her service. I continue to communicate with the lab employees and they always ask .....tell us how your son and daughter are doing and send them our love. The well operated pathology lab is no more dangerous than a high school biology and chemistry laboratory. I believe you would do yourself,the lab.,the pathologist and the 15 year old a great service by reexamining the situation in a more generous and understanding way. If you are at odds with the pathologist.... take time to visit with him and structure a helping and learning experience for the young lady who just happens to be your bosses daughter. Ask the young lady to observe and take notes for a week. Chart the path of a patient specimen from the beginning through the final diagnosis. Write (required) a report of the reason the specimen was sent to the lab. The complete process/path of the specimen through the lab,and the final result/diagnosis for the patient's benefit. If the 15 year old does this there will be a change in the climate and "dad" will be amazed at what you contribute to the process of health care and to the maturing of his daughter. Just a few thoughts from a Pathologist and dad with success written in the log of his children and their bonding to the profession. Sincerely, and in the spirit of helping. Please give a copy of this note to your pathologist. Kindest regards, J.B.McCormick, M.D. jmccormi@schosp.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, June 05, 2008 7:51 AM To: Karla Arrington; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Legality of work Here is how I see your problem: 1- you have already contacted the Child and Labor Dept. about this issue, so it is very likely (if they are doing their job) that the will "pay a visit" to the lab and talk with the pathologist. 2- if that is what is going to happen you are going to be in trouble. 3- as the "youngster's" trainer you are accomplice to the violation, because you know her age and keep training her. 4- if this pathologist is also calling names to others, he is an absolute jerk that thinks is owner, not only of the place, but of the world. 5- I don't think this is the right place to work so sooner or later you should try to find a new place to work. 6- if that is going to be how this whole issue is going to end, I think that you should do what is correct and tell him that you are not willing to participate in violating the laboral laws and stop training his daughter. You will sleep better and perhaps this fellow will realize that he is doing something wrong and that you are not willing to accept the situation. At least that is what I would do. Consult with some close friend or family member and present a formal complaint with Child Labor BEFORE doing #6. If he fires you because of this then you will have PROOF that you complained against him because of a law violation and will be able to claim unemployment, even if he says that you were fired for "work substandard performance", you will have proof that this is not the case, but revenge against you. Good luck! Ren? J. Karla Arrington wrote: Histo's: I have roughly 2 dilemmas. The first is of a legal matter. The Pathologist for a week has had his daughter (15 years old), helping me out in the histology lab. He wants her to get training from me, then do the on-line HT program. She has handled chemicals and reagents. I am very uncomfortable with this. I have called the Child Labor Laws department for our state and it is illegal for a 15 year to be in a laboratory. Needless to say working with blood borne pathogens. My most concern is the following. As her "teacher", can I or other co-workers can be held accountable if this is illegal and is found out? This pathologist mind you is the owner of the business. I am afraid if I say something, I will get fired. Where do I go from here or if there is someone who has a similar circumstance happen to them. The other scenario is that this same Pathologist has called a tech a slandering name, twice. There is no "upper management" to go to since he is the owner of the business. I was wondering if this is considered harassment and can this be used to nullify a contract signed by both parties for employment. Concerned, freckles9660@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From jqb7 <@t> cdc.gov Thu Jun 5 08:27:57 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu Jun 5 08:30:23 2008 Subject: [Histonet] HT practical In-Reply-To: <000101c8c70c$c27372a0$d00f7ca5@lurie.northwestern.edu> References: <005b01c8c694$bf546de0$0302a8c0@yourxhtr8hvc4p> <000101c8c70c$c27372a0$d00f7ca5@lurie.northwestern.edu> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A7F23AB8@LTA3VS011.ees.hhs.gov> I was also told that it was too easy to get someone else to cut and stain your sections. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Thursday, June 05, 2008 9:05 AM To: 'Joe Nocito'; 'Martin, Erin'; 'histonet' Subject: RE: [Histonet] HT practical So why can't slides be sent to reviewers? I always thought the practical was good as it shows a tech can cut (which may be an issue) as well as know staining techniques.how can you troubleshoot micrometry issues if you in theory may or may not be cutting? When I first cam into the field, the first thing done in my interview was to sit me at a microtome and show I could cut.After I was hired I had 3 months to get the quality and quantity of techs with at least 10 years experience on me. Needless to say, I can cut and darn well at that! Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, June 04, 2008 5:46 PM To: Martin, Erin; histonet Subject: Re: [Histonet] HT practical Erin, the practical portion was discontinued a couple of years ago. The exam is computer only. I was told that it was getting too expensive to fly people to Chicago to review the slides. I understand that the questions go into depth and critical thinking and problem solving is a majority of the exam now. JTT ----- Original Message ----- From: "Martin, Erin" To: "histonet" Sent: Wednesday, June 04, 2008 10:59 AM Subject: [Histonet] HT practical Hi everyone, I have two techs who are getting ready to apply for the HT exam but I can't find any info about the practical portion of the exam. Was it discontinued? Or am I not looking in the right place on the ASCP BOR page? Thanks Erin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Thu Jun 5 08:34:01 2008 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Jun 5 08:33:53 2008 Subject: [Histonet] Staining for Hyaluronic acid In-Reply-To: <48470BA1.40406@unsw.edu.au> References: <48470BA1.40406@unsw.edu.au> Message-ID: <4847EB49.30600@umdnj.edu> Fixing the tissue in formalin+alcohol+acetic acid will improve retention of HA and other GAGs. Geoff Rema wrote: > - > > Hi, > > I am trying to stain some rabbit skin to detect Hyaluronic acid but > have found that my staining is very weak even though I have left the > samples in 1% Alcian blue (pH 2.5) for 30 mins which is what standard > procedures from Histo technique books say to do. I am also > counterstaining with neutral red. Does anyone have a procedure that > knows works or could suggest how I can increase the staining intensity? > > Many thanks, > Rema > > ------------------------------------------------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From kgreen <@t> hsh.org Thu Jun 5 08:34:05 2008 From: kgreen <@t> hsh.org (Green, Kathy) Date: Thu Jun 5 08:34:12 2008 Subject: [Histonet] question Message-ID: Dear Fellow Histotechs, Our one pathologists'constantly complains that our frozen section slides are not blue enough. We've tried leaving it in the Hematoxylin 2 longer & we even switched from ammonia water to Blue Buffer by Surgipath. I'm asking what procedures and/or chemicals other labs use in their frozen section staining area. Thanks for any input. Kathy Green, HT Manager Histology Laboratory Holy Spirit Hospital 503 N. 21st Street Camp Hill, PA 17011 kgreen@hsh.org (717) 763-2930 Blackberry: (717) 370-1726 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You From hborgeri <@t> wfubmc.edu Thu Jun 5 08:40:44 2008 From: hborgeri <@t> wfubmc.edu (Hermina Borgerink) Date: Thu Jun 5 08:41:21 2008 Subject: [Histonet] IGF-1 for FFPE Message-ID: <9AEEF1FB6254224AA355ED285F8491652CBFB21F@EXCHVS2.medctr.ad.wfubmc.edu> Good morning all, I am looking for an antibody for IGF-1 to be used on non-human primate FFPE tissue sections. I recently purchased an IGF-1R from Imgenex and tried it on human placenta using 1mM EDTA pH 8.0 (as recommended by the vendor) but my results were not very satisfactory. Before I give up on this particular antibody though, I will try a higher pH to see if that might improve the quality of my staining. Meanwhile, should someone know of an antibody for IGF-1 that works well on paraffin sections ........ Thanking you in advance, Hermina Hermina M. Borgerink, BA, HT, HTL(ASCP)QIHC Wake Forest University Primate Center Department of Pathology Medical Center Blvd. Winston-Salem, NC 27157 Tel. (336) 716-1538 Fax. (336) 716-1515 e-mail: hborgeri@wfubmc.edu "Treat a man as he appears to be, and you make him worse. But treat a man as if he already were what he potentially could be, and you make him what he should be." (Goethe) This electronic message, including any attachments, is confidential and proprietary and is solely for the intended recipient. If you are not the intended recipient, this message was sent to you in error and you are hereby advised that any review, disclosure, copying, distribution or use of this message, or any of the information included therein, is unauthorized and strictly prohibited. If you have received this electronic transmission in error, please immediately notify the sender by reply and permanently delete all copies of this message and its attachments. Thank you. From hhawkins <@t> utmb.edu Thu Jun 5 08:41:51 2008 From: hhawkins <@t> utmb.edu (Hawkins, Hal K.) Date: Thu Jun 5 08:42:08 2008 Subject: [Histonet] Legality of work References: <163789.93086.qm@web32506.mail.mud.yahoo.com><466600.51945.qm@web65704.mail.ac4.yahoo.com> <95D447442469F44DB2A370783169A3E804DB2667@exch01.backup> Message-ID: I also think I am an academic pathologist now because I had the privilege of working in an academic pathology lab one summer when I was 15, in a summer science program in Oklahoma City with other students. It was far more fun than counting money at my father's company all day! I would also suggest that if the student's experience can be structured as an educational project through the local high school or college or technical school, or as a volunteer arrangement with all the appropriate testing and insurance, the chld labor laws would not be a problem. Hal Hawkins, M.D.,Ph.D., UTMB, Galveston -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of McCormick, James Sent: Thu 6/5/2008 8:29 AM To: Rene J Buesa; Karla Arrington; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Legality of work >From an interested Pathologist: I think you are building a bomb to attack an mouse! When our son and daughter were 14 and 15 years old I was proud to have them VOLUNTEER (non-paid) service to the our laboratory. First to meet the fine people who worked in the department. Second to learn about "dad's work", and third to develop a sense of appreciation for our work in serving others. The children benefited by the social relationship and grew in responsibility by graduating from tasks of simple "go for's", observers, awarded responsibility. They were not permitted to prepare patient materials but they were, over a period of summer volunteer effort, taught to make a collection of their own slides and even to do a frozen section. The experience is a treasured memory and valued experience for the Pathologist and his family and for the bonding of employees who for that period were extended members of the family. Our son is now completing his residency in orthopaedic Surgery and our daughter is a junior officer in a New York company where she has employees reporting to her service. I continue to communicate with the lab employees and they always ask .....tell us how your son and daughter are doing and send them our love. The well operated pathology lab is no more dangerous than a high school biology and chemistry laboratory. I believe you would do yourself,the lab.,the pathologist and the 15 year old a great service by reexamining the situation in a more generous and understanding way. If you are at odds with the pathologist.... take time to visit with him and structure a helping and learning experience for the young lady who just happens to be your bosses daughter. Ask the young lady to observe and take notes for a week. Chart the path of a patient specimen from the beginning through the final diagnosis. Write (required) a report of the reason the specimen was sent to the lab. The complete process/path of the specimen through the lab,and the final result/diagnosis for the patient's benefit. If the 15 year old does this there will be a change in the climate and "dad" will be amazed at what you contribute to the process of health care and to the maturing of his daughter. Just a few thoughts from a Pathologist and dad with success written in the log of his children and their bonding to the profession. Sincerely, and in the spirit of helping. Please give a copy of this note to your pathologist. Kindest regards, J.B.McCormick, M.D. jmccormi@schosp.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, June 05, 2008 7:51 AM To: Karla Arrington; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Legality of work Here is how I see your problem: 1- you have already contacted the Child and Labor Dept. about this issue, so it is very likely (if they are doing their job) that the will "pay a visit" to the lab and talk with the pathologist. 2- if that is what is going to happen you are going to be in trouble. 3- as the "youngster's" trainer you are accomplice to the violation, because you know her age and keep training her. 4- if this pathologist is also calling names to others, he is an absolute jerk that thinks is owner, not only of the place, but of the world. 5- I don't think this is the right place to work so sooner or later you should try to find a new place to work. 6- if that is going to be how this whole issue is going to end, I think that you should do what is correct and tell him that you are not willing to participate in violating the laboral laws and stop training his daughter. You will sleep better and perhaps this fellow will realize that he is doing something wrong and that you are not willing to accept the situation. At least that is what I would do. Consult with some close friend or family member and present a formal complaint with Child Labor BEFORE doing #6. If he fires you because of this then you will have PROOF that you complained against him because of a law violation and will be able to claim unemployment, even if he says that you were fired for "work substandard performance", you will have proof that this is not the case, but revenge against you. Good luck! Ren? J. Karla Arrington wrote: Histo's: I have roughly 2 dilemmas. The first is of a legal matter. The Pathologist for a week has had his daughter (15 years old), helping me out in the histology lab. He wants her to get training from me, then do the on-line HT program. She has handled chemicals and reagents. I am very uncomfortable with this. I have called the Child Labor Laws department for our state and it is illegal for a 15 year to be in a laboratory. Needless to say working with blood borne pathogens. My most concern is the following. As her "teacher", can I or other co-workers can be held accountable if this is illegal and is found out? This pathologist mind you is the owner of the business. I am afraid if I say something, I will get fired. Where do I go from here or if there is someone who has a similar circumstance happen to them. The other scenario is that this same Pathologist has called a tech a slandering name, twice. There is no "upper management" to go to since he is the owner of the business. I was wondering if this is considered harassment and can this be used to nullify a contract signed by both parties for employment. Concerned, freckles9660@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Jun 5 08:44:47 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jun 5 08:44:55 2008 Subject: [Histonet] Legality of work In-Reply-To: <95D447442469F44DB2A370783169A3E804DB2667@exch01.backup> Message-ID: <677716.83847.qm@web65713.mail.ac4.yahoo.com> Dear Dr. McCormick: Your answer/comments are not only "from an interested pathologist", but also from one of the most decent persons in this trade, somebody who we all look up to. That is what YOU would do, that is what YOU would accept if Karla would approach this problem that way, but it seems that the pathologist in this case is much much less "inclined" to act like you, when he has insulted some of Karla's coworkers, something I can bet YOU have never done. Perhaps the similitude between bomb and mouse only applies to the mouse part of it. Ren? J. "McCormick, James" wrote: From an interested Pathologist: I think you are building a bomb to attack an mouse! When our son and daughter were 14 and 15 years old I was proud to have them VOLUNTEER (non-paid) service to the our laboratory. First to meet the fine people who worked in the department. Second to learn about "dad's work", and third to develop a sense of appreciation for our work in serving others. The children benefited by the social relationship and grew in responsibility by graduating from tasks of simple "go for's", observers, awarded responsibility. They were not permitted to prepare patient materials but they were, over a period of summer volunteer effort, taught to make a collection of their own slides and even to do a frozen section. The experience is a treasured memory and valued experience for the Pathologist and his family and for the bonding of employees who for that period were extended members of the family. Our son is now completing his residency in orthopaedic Surgery and our daughter is a junior officer in a New York company where she has employees reporting to her service. I continue to communicate with the lab employees and they always ask .....tell us how your son and daughter are doing and send them our love. The well operated pathology lab is no more dangerous than a high school biology and chemistry laboratory. I believe you would do yourself,the lab.,the pathologist and the 15 year old a great service by reexamining the situation in a more generous and understanding way. If you are at odds with the pathologist.... take time to visit with him and structure a helping and learning experience for the young lady who just happens to be your bosses daughter. Ask the young lady to observe and take notes for a week. Chart the path of a patient specimen from the beginning through the final diagnosis. Write (required) a report of the reason the specimen was sent to the lab. The complete process/path of the specimen through the lab,and the final result/diagnosis for the patient's benefit. If the 15 year old does this there will be a change in the climate and "dad" will be amazed at what you contribute to the process of health care and to the maturing of his daughter. Just a few thoughts from a Pathologist and dad with success written in the log of his children and their bonding to the profession. Sincerely, and in the spirit of helping. Please give a copy of this note to your pathologist. Kindest regards, J.B.McCormick, M.D. jmccormi@schosp.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, June 05, 2008 7:51 AM To: Karla Arrington; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Legality of work Here is how I see your problem: 1- you have already contacted the Child and Labor Dept. about this issue, so it is very likely (if they are doing their job) that the will "pay a visit" to the lab and talk with the pathologist. 2- if that is what is going to happen you are going to be in trouble. 3- as the "youngster's" trainer you are accomplice to the violation, because you know her age and keep training her. 4- if this pathologist is also calling names to others, he is an absolute jerk that thinks is owner, not only of the place, but of the world. 5- I don't think this is the right place to work so sooner or later you should try to find a new place to work. 6- if that is going to be how this whole issue is going to end, I think that you should do what is correct and tell him that you are not willing to participate in violating the laboral laws and stop training his daughter. You will sleep better and perhaps this fellow will realize that he is doing something wrong and that you are not willing to accept the situation. At least that is what I would do. Consult with some close friend or family member and present a formal complaint with Child Labor BEFORE doing #6. If he fires you because of this then you will have PROOF that you complained against him because of a law violation and will be able to claim unemployment, even if he says that you were fired for "work substandard performance", you will have proof that this is not the case, but revenge against you. Good luck! Ren? J. Karla Arrington wrote: Histo's: I have roughly 2 dilemmas. The first is of a legal matter. The Pathologist for a week has had his daughter (15 years old), helping me out in the histology lab. He wants her to get training from me, then do the on-line HT program. She has handled chemicals and reagents. I am very uncomfortable with this. I have called the Child Labor Laws department for our state and it is illegal for a 15 year to be in a laboratory. Needless to say working with blood borne pathogens. My most concern is the following. As her "teacher", can I or other co-workers can be held accountable if this is illegal and is found out? This pathologist mind you is the owner of the business. I am afraid if I say something, I will get fired. Where do I go from here or if there is someone who has a similar circumstance happen to them. The other scenario is that this same Pathologist has called a tech a slandering name, twice. There is no "upper management" to go to since he is the owner of the business. I was wondering if this is considered harassment and can this be used to nullify a contract signed by both parties for employment. Concerned, freckles9660@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From JMahoney <@t> alegent.org Thu Jun 5 08:46:59 2008 From: JMahoney <@t> alegent.org (Mahoney,Janice A) Date: Thu Jun 5 08:48:01 2008 Subject: [Histonet] Legality of work In-Reply-To: <466600.51945.qm@web65704.mail.ac4.yahoo.com> References: <163789.93086.qm@web32506.mail.mud.yahoo.com> <466600.51945.qm@web65704.mail.ac4.yahoo.com> Message-ID: <346E5878979BA54FB4B0BFD6AD93B9B9B01F623363@EXCHMBC1.ad.ah.local> Excellent advice Rene! Jan Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, June 05, 2008 7:51 AM To: Karla Arrington; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Legality of work Here is how I see your problem: 1- you have already contacted the Child and Labor Dept. about this issue, so it is very likely (if they are doing their job) that the will "pay a visit" to the lab and talk with the pathologist. 2- if that is what is going to happen you are going to be in trouble. 3- as the "youngster's" trainer you are accomplice to the violation, because you know her age and keep training her. 4- if this pathologist is also calling names to others, he is an absolute jerk that thinks is owner, not only of the place, but of the world. 5- I don't think this is the right place to work so sooner or later you should try to find a new place to work. 6- if that is going to be how this whole issue is going to end, I think that you should do what is correct and tell him that you are not willing to participate in violating the laboral laws and stop training his daughter. You will sleep better and perhaps this fellow will realize that he is doing something wrong and that you are not willing to accept the situation. At least that is what I would do. Consult with some close friend or family member and present a formal complaint with Child Labor BEFORE doing #6. If he fires you because of this then you will have PROOF that you complained against him because of a law violation and will be able to claim unemployment, even if he says that you were fired for "work substandard performance", you will have proof that this is not the case, but revenge against you. Good luck! Ren? J. Karla Arrington wrote: Histo's: I have roughly 2 dilemmas. The first is of a legal matter. The Pathologist for a week has had his daughter (15 years old), helping me out in the histology lab. He wants her to get training from me, then do the on-line HT program. She has handled chemicals and reagents. I am very uncomfortable with this. I have called the Child Labor Laws department for our state and it is illegal for a 15 year to be in a laboratory. Needless to say working with blood borne pathogens. My most concern is the following. As her "teacher", can I or other co-workers can be held accountable if this is illegal and is found out? This pathologist mind you is the owner of the business. I am afraid if I say something, I will get fired. Where do I go from here or if there is someone who has a similar circumstance happen to them. The other scenario is that this same Pathologist has called a tech a slandering name, twice. There is no "upper management" to go to since he is the owner of the business. I was wondering if this is considered harassment and can this be used to nullify a contract signed by both parties for employment. Concerned, freckles9660@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From bakevictoria <@t> gmail.com Thu Jun 5 08:56:26 2008 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Thu Jun 5 08:56:42 2008 Subject: [Histonet] question In-Reply-To: References: Message-ID: <4f016b690806050656g22837903o61232b5f843c2901@mail.gmail.com> Kathy, For bluing reagents I've used several, ammonia water, saturated lithium carbonate, 0.5% lithium carbonate and Scott's solution/water. The Scott's solution was from Polyscientific in Bayshore NY and it's a modified formula. The original one I used, I think (I don't have it in front of me) was from Sheehan. What I found was most important with the nuclear staining was that the hematoxylin had to as fresh as possible and that the most critical steps were the removal of the OCT media prior to staining as that seemed to give the sections that greyish watery appearance. Also, are you differentiating prior to bluing? Just asking as you didn't say what your protocol was. Vikki On 6/5/08, Green, Kathy wrote: > Dear Fellow Histotechs, > > Our one pathologists'constantly complains that our frozen section slides > are not blue enough. We've tried leaving it in the Hematoxylin 2 longer > & we even switched from ammonia water to Blue Buffer by Surgipath. I'm > asking what procedures and/or chemicals other labs use in their frozen > section staining area. Thanks for any input. > > > > Kathy Green, HT > > Manager Histology Laboratory > > Holy Spirit Hospital > > 503 N. 21st Street > > Camp Hill, PA 17011 > > kgreen@hsh.org > > (717) 763-2930 > > Blackberry: (717) 370-1726 > > > > > > Confidentiality Disclaimer: The information contained in this communication may be confidential, > is intended for the use of the recipient named above, and may be legally privileged.If the reader > of this message is not the intended recipient, you are hereby notified that any dissemination, > distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you > received this communication in error, please resend this communication to the sender and delete > the original message and any copy of it from your computer system. Thank You > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Stacy_McLaughlin <@t> cooley-dickinson.org Thu Jun 5 09:03:02 2008 From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin) Date: Thu Jun 5 09:03:10 2008 Subject: [Histonet] Hematoxylin shortage? Message-ID: I know this subject has come up before, but I'm wondering if this is something people are still having a problem with? Which vendors have not been effected? Thanks, Stacy McLaughlin From dusko.trajkovic <@t> pfizer.com Thu Jun 5 09:09:28 2008 From: dusko.trajkovic <@t> pfizer.com (Trajkovic, Dusko) Date: Thu Jun 5 09:09:45 2008 Subject: [Histonet] mouse heart chatter upon sectioning... In-Reply-To: <447056A67472B241A330A525B4AF716720621B@EX02.ad.tulane.edu> Message-ID: <3AD0BD3142459B4E9B12CBEAFF2B89B20736715A@lajamrexm01.amer.pfizer.com> I have worked with animal tissues for well over 18 years and I agree with other previous posters here, that your problem is most likely the fixation at 4oC. Dusko Trajkovic -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gaupp, Dina D Sent: Wednesday, June 04, 2008 4:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] mouse heart chatter upon sectioning... Whenever I process (whole)mouse hearts, the tissue chatters upon sectioning. The tissue is usually fixed overnight in 10%nbf at 4C before processed. I tried different processing times. The last time I processed hearts, I processed them for 5 hours total no vaccum. Should I decrease the processing time even lower than that? I am afraid of underprocessing. I have to section the entire heart from top to bottom, so I really need some advice. (open system) Processor Set Up: 1 - fix (if tissue fixed overnight, skip #1 & #2 stations) 2 - fix 3 - 70% 4 - 80% 5 & 6 - 95% 7 & 8 - 100% 9 & 10- Xylene Sub. - Clear-rite 11 & 12-Paraffin stations Thanks, Dina dgaupp@tulane.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vazquezr <@t> ohsu.edu Thu Jun 5 08:56:41 2008 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Thu Jun 5 09:10:05 2008 Subject: [Histonet] question Message-ID: Karen, I just put a good scoop of good ol' baking soda, instead of ammonia water. Never a problem. Robyn >>> "Green, Kathy" 6/5/2008 6:34 AM >>> Dear Fellow Histotechs, Our one pathologists'constantly complains that our frozen section slides are not blue enough. We've tried leaving it in the Hematoxylin 2 longer & we even switched from ammonia water to Blue Buffer by Surgipath. I'm asking what procedures and/or chemicals other labs use in their frozen section staining area. Thanks for any input. Kathy Green, HT Manager Histology Laboratory Holy Spirit Hospital 503 N. 21st Street Camp Hill, PA 17011 kgreen@hsh.org (717) 763-2930 Blackberry: (717) 370-1726 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Thu Jun 5 09:19:51 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Jun 5 09:14:12 2008 Subject: [Histonet] Legality of work In-Reply-To: <95D447442469F44DB2A370783169A3E804DB2667@exch01.backup> References: <163789.93086.qm@web32506.mail.mud.yahoo.com><466600.51945.qm@web65704.mail.ac4.yahoo.com> <95D447442469F44DB2A370783169A3E804DB2667@exch01.backup> Message-ID: <1F937FB30BDB7C4A9F39F83FEA8D379F9F555F@bruexchange1.digestivespecialists.com> As a 15 and 16 year old I spent summers in a histo/cyto lab and fell in love (with the lab). I have been in the lab ever since and I'm old now! The techs and the pathologist took time with me and showed me a world that I would never have known. Both of my children had the opportunity to spend some time in the lab as well as my grand daughter. They have all benefited from the experience. I think you have the opportunity to show the pathologist daughter something important even if he is a jerk. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McCormick, James Sent: Thursday, June 05, 2008 9:29 AM To: Rene J Buesa; Karla Arrington; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Legality of work >From an interested Pathologist: I think you are building a bomb to attack an mouse! When our son and daughter were 14 and 15 years old I was proud to have them VOLUNTEER (non-paid) service to the our laboratory. First to meet the fine people who worked in the department. Second to learn about "dad's work", and third to develop a sense of appreciation for our work in serving others. The children benefited by the social relationship and grew in responsibility by graduating from tasks of simple "go for's", observers, awarded responsibility. They were not permitted to prepare patient materials but they were, over a period of summer volunteer effort, taught to make a collection of their own slides and even to do a frozen section. The experience is a treasured memory and valued experience for the Pathologist and his family and for the bonding of employees who for that period were extended members of the family. Our son is now completing his residency in orthopaedic Surgery and our daughter is a junior officer in a New York company where she has employees reporting to her service. I continue to communicate with the lab employees and they always ask .....tell us how your son and daughter are doing and send them our love. The well operated pathology lab is no more dangerous than a high school biology and chemistry laboratory. I believe you would do yourself,the lab.,the pathologist and the 15 year old a great service by reexamining the situation in a more generous and understanding way. If you are at odds with the pathologist.... take time to visit with him and structure a helping and learning experience for the young lady who just happens to be your bosses daughter. Ask the young lady to observe and take notes for a week. Chart the path of a patient specimen from the beginning through the final diagnosis. Write (required) a report of the reason the specimen was sent to the lab. The complete process/path of the specimen through the lab,and the final result/diagnosis for the patient's benefit. If the 15 year old does this there will be a change in the climate and "dad" will be amazed at what you contribute to the process of health care and to the maturing of his daughter. Just a few thoughts from a Pathologist and dad with success written in the log of his children and their bonding to the profession. Sincerely, and in the spirit of helping. Please give a copy of this note to your pathologist. Kindest regards, J.B.McCormick, M.D. jmccormi@schosp.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, June 05, 2008 7:51 AM To: Karla Arrington; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Legality of work Here is how I see your problem: 1- you have already contacted the Child and Labor Dept. about this issue, so it is very likely (if they are doing their job) that the will "pay a visit" to the lab and talk with the pathologist. 2- if that is what is going to happen you are going to be in trouble. 3- as the "youngster's" trainer you are accomplice to the violation, because you know her age and keep training her. 4- if this pathologist is also calling names to others, he is an absolute jerk that thinks is owner, not only of the place, but of the world. 5- I don't think this is the right place to work so sooner or later you should try to find a new place to work. 6- if that is going to be how this whole issue is going to end, I think that you should do what is correct and tell him that you are not willing to participate in violating the laboral laws and stop training his daughter. You will sleep better and perhaps this fellow will realize that he is doing something wrong and that you are not willing to accept the situation. At least that is what I would do. Consult with some close friend or family member and present a formal complaint with Child Labor BEFORE doing #6. If he fires you because of this then you will have PROOF that you complained against him because of a law violation and will be able to claim unemployment, even if he says that you were fired for "work substandard performance", you will have proof that this is not the case, but revenge against you. Good luck! Ren? J. Karla Arrington wrote: Histo's: I have roughly 2 dilemmas. The first is of a legal matter. The Pathologist for a week has had his daughter (15 years old), helping me out in the histology lab. He wants her to get training from me, then do the on-line HT program. She has handled chemicals and reagents. I am very uncomfortable with this. I have called the Child Labor Laws department for our state and it is illegal for a 15 year to be in a laboratory. Needless to say working with blood borne pathogens. My most concern is the following. As her "teacher", can I or other co-workers can be held accountable if this is illegal and is found out? This pathologist mind you is the owner of the business. I am afraid if I say something, I will get fired. Where do I go from here or if there is someone who has a similar circumstance happen to them. The other scenario is that this same Pathologist has called a tech a slandering name, twice. There is no "upper management" to go to since he is the owner of the business. I was wondering if this is considered harassment and can this be used to nullify a contract signed by both parties for employment. Concerned, freckles9660@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgalnow <@t> aol.com Thu Jun 5 09:17:18 2008 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Thu Jun 5 09:17:43 2008 Subject: [Histonet] Legality of work In-Reply-To: <163789.93086.qm@web32506.mail.mud.yahoo.com> References: <163789.93086.qm@web32506.mail.mud.yahoo.com> Message-ID: <8CA952DFEE260A5-82C-FA0@FWM-M28.sysops.aol.com> Shaky ground.? It is very difficult to work so closely in a private owned facility.? There seems to be "no rules" the ownership has to follow---even with management there.? The child is very scary...but when the fines come it won't be you that has to pay them.? Can you get in touch with her school and have her be signed up for a shadowing program?? This may relieve some of the "legality issues". Roxanne -----Original Message----- From: Karla Arrington To: histonet@lists.utsouthwestern.edu Sent: Wed, 4 Jun 2008 7:37 pm Subject: [Histonet] Legality of work Histo's: ? I have roughly 2 dilemmas.? The first is of a legal matter.? The Pathologist for a week has had his daughter (15 years old), helping me out in the histology lab.? He wants her to get training from me, then do the on-line HT program.? She has handled chemicals and reagents.? I am very uncomfortable with this.? I have called the Child Labor Laws department for our state and it is illegal for a 15 year to be in a laboratory. Needless to say working with blood borne pathogens.? My most concern is the following.? As her "teacher", can I or other co-workers can be held accountable if this is illegal and is found out??This pathologist mind you is the owner of the business.? I am afraid if I say something, I will get fired. Where do I go from here or if there is someone who has a similar circumstance happen to them. The other scenario is that this same Pathologist has called a tech a slandering name, twice.? There is no "upper management" to go to since he is the owner of the business. I was wondering if this is considered harassment and can this be used to?nullify a contract signed by both parties for employment. ? Concerned, freckles9660@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> u.arizona.edu Thu Jun 5 09:54:23 2008 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Thu Jun 5 10:04:18 2008 Subject: [Histonet] Legality of work In-Reply-To: <1F937FB30BDB7C4A9F39F83FEA8D379F9F555F@bruexchange1.digest ivespecialists.com> References: <163789.93086.qm@web32506.mail.mud.yahoo.com> <466600.51945.qm@web65704.mail.ac4.yahoo.com> <95D447442469F44DB2A370783169A3E804DB2667@exch01.backup> <1F937FB30BDB7C4A9F39F83FEA8D379F9F555F@bruexchange1.digestivespecialists.com> Message-ID: <6.2.3.4.1.20080605074122.01f63690@algranth.inbox.email.arizona.edu> I have to second what Linda has posted. When I was in high school I used to go to work with my Mom in the summer. She worked as a unit clerk at our local hospital in McKeesport. She had a friend who was the supervisor of the histology lab and she let me come down to "see" what went on there. After that I always wanted to work in a lab and I did and I still am and I'm old too! My daughter was a Candystriper when she was in high school and she would come to work with me and until her job started she would come to my lab and help out by labeling, logging, etc. She got to see what Mom did and like Dr. McCormick I think it was a good thing having your kids understand what it is that you do everyday at work. Now for the jerk thing - I've been there too and I admit that you are between a rock and a hard place. I prayed for the guy and it ended up he got fired! Never estimate the power of prayer? Andi At 07:19 AM 6/5/2008, Blazek, Linda wrote: >As a 15 and 16 year old I spent summers in a >histo/cyto lab and fell in love (with the >lab). I have been in the lab ever since and I'm >old now! The techs and the pathologist took >time with me and showed me a world that I would >never have known. Both of my children had the >opportunity to spend some time in the lab as >well as my grand daughter. They have all >benefited from the experience. I think you have >the opportunity to show the pathologist daughter >something important even if he is a jerk. > > >Linda Blazek HT (ASCP) >Manager/Supervisor >GI Pathology of Dayton >7415 Brandt Pike >Huber Heights, OH 45424 >Phone: (937) 293-4424 ext 7118 >Email: lblazek@digestivespecialists.com > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] >On Behalf Of McCormick, James >Sent: Thursday, June 05, 2008 9:29 AM >To: Rene J Buesa; Karla Arrington; histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] Legality of work > > >From an interested Pathologist: >I think you are building a bomb to attack an mouse! > >When our son and daughter were 14 and 15 years >old I was proud to have them VOLUNTEER >(non-paid) service to the our laboratory. First >to meet the fine people who worked in the >department. Second to learn about "dad's work", >and third to develop a sense of appreciation for our work in serving others. >The children benefited by the social >relationship and grew in responsibility by >graduating from tasks of simple "go for's", observers, awarded responsibility. >They were not permitted to prepare patient >materials but they were, over a period of summer >volunteer effort, taught to make a collection of >their own slides and even to do a frozen section. >The experience is a treasured memory and valued >experience for the Pathologist and his family >and for the bonding of employees who for that >period were extended members of the family. >Our son is now completing his residency in >orthopaedic Surgery and our daughter is a junior >officer in a New York company where she has >employees reporting to her service. >I continue to communicate with the lab employees >and they always ask .....tell us how your son >and daughter are doing and send them our love. >The well operated pathology lab is no more >dangerous than a high school biology and chemistry laboratory. > >I believe you would do yourself,the lab.,the >pathologist and the 15 year old a great service >by reexamining the situation in a more generous >and understanding way. If you are at odds with >the pathologist.... take time to visit with him >and structure a helping and learning experience >for the young lady who just happens to be your bosses daughter. >Ask the young lady to observe and take notes for >a week. Chart the path of a patient specimen >from the beginning through the final diagnosis. >Write (required) a report of the reason the >specimen was sent to the lab. The complete >process/path of the specimen through the lab,and >the final result/diagnosis for the patient's benefit. > >If the 15 year old does this there will be a >change in the climate and "dad" will be amazed >at what you contribute to the process of health >care and to the maturing of his daughter. > >Just a few thoughts from a Pathologist and dad >with success written in the log of his children >and their bonding to the profession. > >Sincerely, and in the spirit of helping. >Please give a copy of this note to your pathologist. > >Kindest regards, >J.B.McCormick, M.D. >jmccormi@schosp.org > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa >Sent: Thursday, June 05, 2008 7:51 AM >To: Karla Arrington; histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] Legality of work > >Here is how I see your problem: > 1- you have already contacted the Child and > Labor Dept. about this issue, so it is very > likely (if they are doing their job) that the > will "pay a visit" to the lab and talk with the pathologist. > 2- if that is what is going to happen you are going to be in trouble. > 3- as the "youngster's" trainer you are > accomplice to the violation, because you know her age and keep training her. > 4- if this pathologist is also calling names > to others, he is an absolute jerk that thinks > is owner, not only of the place, but of the world. > 5- I don't think this is the right place to > work so sooner or later you should try to find a new place to work. > 6- if that is going to be how this whole > issue is going to end, I think that you should > do what is correct and tell him that you are > not willing to participate in violating the > laboral laws and stop training his daughter. > You will sleep better and perhaps this fellow > will realize that he is doing something wrong > and that you are not willing to accept the situation. > At least that is what I would do. Consult > with some close friend or family member and > present a formal complaint with Child Labor > BEFORE doing #6. If he fires you because of > this then you will have PROOF that you > complained against him because of a law > violation and will be able to claim > unemployment, even if he says that you were > fired for "work substandard performance", you > will have proof that this is not the case, but revenge against you. > Good luck! >Ren? J. > >Karla Arrington wrote: > Histo's: > >I have roughly 2 dilemmas. The first is of a >legal matter. The Pathologist for a week has >had his daughter (15 years old), helping >me out in the histology lab. He wants her to >get training from me, then do the on-line HT >program. She has handled chemicals >and reagents. I am very uncomfortable with >this. I have called the Child Labor Laws >department for our state and it is illegal >for a 15 year to be in a laboratory. Needless to >say working with blood borne pathogens. My most >concern is the following. As her "teacher", >can I or other co-workers can be held >accountable if this is illegal and is found out? >This pathologist mind you is the owner of the business. >I am afraid if I say something, I will get >fired. Where do I go from here or if there is >someone who has a similar circumstance happen to them. >The other scenario is that this same Pathologist >has called a tech a slandering name, >twice. There is no "upper management" to go to >since he is the owner of the business. I was >wondering if this is considered harassment and >can this be used to nullify a contract signed >by both parties for employment. > >Concerned, >freckles9660@yahoo.com > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >*** Confidentiality Statement *** >This e-mail is intended only for the use of the >individual or entity to which it is addressed >and may contain information that is privileged >and confidential. If the reader of this message >is not the intended recipient, please notify the >sender immediately by replying to this message >and then delete it from your system. Any review, >dissemination, distribution, or reproduction of >this message by unintended recipients is >strictly prohibited and may be subject to legal restriction. > > >Thank you for your cooperation. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From crochieresteve <@t> aol.com Thu Jun 5 10:20:28 2008 From: crochieresteve <@t> aol.com (crochieresteve@aol.com) Date: Thu Jun 5 10:20:54 2008 Subject: [Histonet] salary scale Message-ID: <8CA9536D2368219-EE4-48D@FWM-M18.sysops.aol.com> In an attempt to see if our scale is up to par, does anyone wish to share the pay scale for entry, middle level and experienced histo toechs with me? thanks steve From Xilong.Li <@t> UTSouthwestern.edu Thu Jun 5 10:29:34 2008 From: Xilong.Li <@t> UTSouthwestern.edu (Xilong Li) Date: Thu Jun 5 10:29:44 2008 Subject: [Histonet] Diff Quick Stain Kit Message-ID: <4847BE70.EA4F.00F0.0@UTSouthwestern.edu> Hi, All, I am wondering is there any guys who use Diff-Quick Stain kit from IMEB Inc? Our lab use this kit to stain cells spinned down on the slide from bronchoalveolar lavage. We want to distinguish the changes of lymphocyte and neutrophil, we also want to include eosinophil if possible. The results were not stable, some cells were stained too light, some cells on another slide were too dark, at most of time, it is very hard to distinguish the cell type. Please let me know if you have any experience in the staining those kind of cells by Diff-Quick Stain kit or other kit. Any suggestion would be appreciated. Thanks in advance. Xilong Dr. Xilong Li Hypertension Division, Internal Medicine University of Texas Southwestern Medical Center 5323 Harry Hiness Blvd-J4.142 Dallas, TX 75390 Tel: 214-648-9966(L) Fax: 214-648-7902 From petepath <@t> yahoo.com Thu Jun 5 10:34:29 2008 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Thu Jun 5 10:34:33 2008 Subject: [Histonet] question Message-ID: <343243.51820.qm@web45110.mail.sp1.yahoo.com> Kathy, You may also want to consider the thickness of your sections. A great deal of our information is gatered at scanning magnification of 2x or 4x. If you are cutting at 3 or 4 microns or if your cryostat is offering a variety of thick and thin the slides will be pale at these powers compared to 5 or 6 micron sections. Presence of a lot of nuclear "holes"is a sign it is cut very thin. Also make sure your hematoxalin is changed regularly and is not growing "rock candy" in the bottom. Check slide after it leaves the bluing and get to know the shade of blue that represents a well stained slide. Remember when checking the slides, the amount of blue will depend on the densityof nuclear material as well as the thickness of the tissue. I find the actual shade of blue tells me that it has been stained in hemotoxalin long enough. Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com From ploykasek <@t> phenopath.com Thu Jun 5 10:40:02 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Thu Jun 5 10:40:13 2008 Subject: [Histonet] Placement help Message-ID: HI all. I have a question for a friend. Does anyone know of a job placement service that specializes in doctorate level candidates? I would think there would be one out there somewhere, but haven't come across it. Thank you. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From b-frederick <@t> northwestern.edu Thu Jun 5 11:14:50 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Jun 5 11:15:07 2008 Subject: [Histonet] Legality of work In-Reply-To: <466600.51945.qm@web65704.mail.ac4.yahoo.com> Message-ID: <000001c8c727$4b476040$d00f7ca5@lurie.northwestern.edu> Not to mention that 15 year olds would not even have the education to do the on-line program. There are requirements as we all know. Scary, scary ,scary. Does this pathologist know the meaning of malpractice and wouldn't the AMA be of help in this as well as ASCP? Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, June 05, 2008 7:51 AM To: Karla Arrington; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Legality of work Here is how I see your problem: 1- you have already contacted the Child and Labor Dept. about this issue, so it is very likely (if they are doing their job) that the will "pay a visit" to the lab and talk with the pathologist. 2- if that is what is going to happen you are going to be in trouble. 3- as the "youngster's" trainer you are accomplice to the violation, because you know her age and keep training her. 4- if this pathologist is also calling names to others, he is an absolute jerk that thinks is owner, not only of the place, but of the world. 5- I don't think this is the right place to work so sooner or later you should try to find a new place to work. 6- if that is going to be how this whole issue is going to end, I think that you should do what is correct and tell him that you are not willing to participate in violating the laboral laws and stop training his daughter. You will sleep better and perhaps this fellow will realize that he is doing something wrong and that you are not willing to accept the situation. At least that is what I would do. Consult with some close friend or family member and present a formal complaint with Child Labor BEFORE doing #6. If he fires you because of this then you will have PROOF that you complained against him because of a law violation and will be able to claim unemployment, even if he says that you were fired for "work substandard performance", you will have proof that this is not the case, but revenge against you. Good luck! Ren? J. Karla Arrington wrote: Histo's: I have roughly 2 dilemmas. The first is of a legal matter. The Pathologist for a week has had his daughter (15 years old), helping me out in the histology lab. He wants her to get training from me, then do the on-line HT program. She has handled chemicals and reagents. I am very uncomfortable with this. I have called the Child Labor Laws department for our state and it is illegal for a 15 year to be in a laboratory. Needless to say working with blood borne pathogens. My most concern is the following. As her "teacher", can I or other co-workers can be held accountable if this is illegal and is found out? This pathologist mind you is the owner of the business. I am afraid if I say something, I will get fired. Where do I go from here or if there is someone who has a similar circumstance happen to them. The other scenario is that this same Pathologist has called a tech a slandering name, twice. There is no "upper management" to go to since he is the owner of the business. I was wondering if this is considered harassment and can this be used to nullify a contract signed by both parties for employment. Concerned, freckles9660@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Thu Jun 5 11:22:30 2008 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Jun 5 11:22:35 2008 Subject: [Histonet] Slightly OT: Lab kids Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E636C@nmdamailsvr.nmda.ad.nmsu.edu> I second Linda Blazek's, and other's, posts about working in a lab as a teen. Our hospital was too small for Candystripers and I wanted in on the "action", so I pestered the med tech until he offered me a job at 50 cents/hour out of his own pocket. I got to wash urine bottles and Vacutainer tubes and make culture media but I graduated to doing EKGs, running the autoclave in the surgery suite, and a hundred other things. There was no histo lab and I had no idea what histo was! After a couple years of college and when the hospital finally hired a pathologist/lab director, she asked me to place an ad in the local paper for a "histotech" and when she told me what it was, I told her she didn't need to place an ad - I wanted to do it. She sent me to Roswell (yes - that Roswell - which might explain a lot about me...) to work with a histotech for 30 days. When I returned, we ordered all the equipment and I was up and running. That was 1968 and here I am - OLD like Linda and having just as much fun. How coincidental that so many of us Experienced, Well-Seasoned HTs got our start purely by accident! All of us E,W-Ss (Experienced...) should contribute Funny Early Histology Stories and I'll get it into book form! At last - an idea for my profitable retirement! Okay - back to work! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From Janet.Bonner <@t> FLHOSP.ORG Thu Jun 5 11:32:31 2008 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Thu Jun 5 11:33:55 2008 Subject: [Histonet] question References: Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F269D@fhosxchmb006.ADVENTISTCORP.NET> ...Or a Lithium Carbonate Solution ( 24 gm/3500ml) @:) ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Robyn Vazquez Sent: Thu 6/5/2008 9:56 AM To: kgreen@hsh.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] question Karen, I just put a good scoop of good ol' baking soda, instead of ammonia water. Never a problem. Robyn >>> "Green, Kathy" 6/5/2008 6:34 AM >>> Dear Fellow Histotechs, Our one pathologists'constantly complains that our frozen section slides are not blue enough. We've tried leaving it in the Hematoxylin 2 longer & we even switched from ammonia water to Blue Buffer by Surgipath. I'm asking what procedures and/or chemicals other labs use in their frozen section staining area. Thanks for any input. Kathy Green, HT Manager Histology Laboratory Holy Spirit Hospital 503 N. 21st Street Camp Hill, PA 17011 kgreen@hsh.org (717) 763-2930 Blackberry: (717) 370-1726 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From marktarango <@t> gmail.com Thu Jun 5 12:03:12 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Jun 5 12:03:26 2008 Subject: [Histonet] Slightly OT: Lab kids In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E636C@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B017E636C@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <5b6eb13e0806051003w1e466c70v878e47bf0159f8d8@mail.gmail.com> I got my start in the histology lab in high school, and I'm 28 now. The techs tried hard to keep me out of the lab, since I was just the courier at the time. I don't even think it was legal to be the courier at 16, but the pathologist who owned the lab knew I had a driver's licence (and he liked me since I was science club president lol). I worked in another lab that was owned by the histotech when I was 17-19. She would have her elderly mother (in her 90's) wash and re-fill formalin bottles. I can't say how many times I walked into the lab and find her mom squirting herself with formalin and not even realizing it (thinking it was ending up in the bottle). Her mom was far more dangerous in the lab than I was, except for the time I set the lab on fire! (but it didn't burn down...) On Thu, Jun 5, 2008 at 9:22 AM, Breeden, Sara wrote: > I second Linda Blazek's, and other's, posts about working in a lab as a > teen. Our hospital was too small for Candystripers and I wanted in on > the "action", so I pestered the med tech until he offered me a job at 50 > cents/hour out of his own pocket. I got to wash urine bottles and > Vacutainer tubes and make culture media but I graduated to doing EKGs, > running the autoclave in the surgery suite, and a hundred other things. > There was no histo lab and I had no idea what histo was! After a couple > years of college and when the hospital finally hired a pathologist/lab > director, she asked me to place an ad in the local paper for a > "histotech" and when she told me what it was, I told her she didn't need > to place an ad - I wanted to do it. She sent me to Roswell (yes - that > Roswell - which might explain a lot about me...) to work with a > histotech for 30 days. When I returned, we ordered all the equipment and > I was up and running. That was 1968 and here I am - OLD like Linda and > having just as much fun. How coincidental that so many of us > Experienced, Well-Seasoned HTs got our start purely by accident! All of > us E,W-Ss (Experienced...) should contribute Funny Early Histology > Stories and I'll get it into book form! At last - an idea for my > profitable retirement! Okay - back to work! > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From RSRICHMOND <@t> aol.com Thu Jun 5 12:11:54 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Thu Jun 5 12:12:03 2008 Subject: [Histonet] Re: Histonet question, bluing frozen sections Message-ID: Several things to check if frozen section slides appear inadequately blued: The hematoxylin needs to be fresh, filtered daily, and covered when not in use. Don't use a regressive (acid-differentiated) hematoxylin for frozen sections. Slosh the slide around in the hematoxylin until it's thoroughly wetted and doesn't "bead" on the slide. Stain for long enough (usually about a minute). Lithium carbonate is the simplest and strongest bluing agent for frozen sections, though the others work also. Put dry lithium carbonate into water in a bottle (not glass-stoppered) until a fine white sediment appears on the bottom - that means that the LiCO3, which is sparingly soluble in water, is saturated. Pour some off into a Coplin jar and top off the bottle with more water (tap water will usually do). Lithium carbonate has some medical uses, but it is not a controlled substance. When I'm on the road, I carry a supply with me to use in labs where the frozen section bluing is inadequate. Be sure to look at the slide yourself! Bob Richmond Samurai Pathologist Knoxville TN From Jackie.O'Connor <@t> abbott.com Thu Jun 5 12:20:23 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Jun 5 12:20:45 2008 Subject: [Histonet] Legality of work In-Reply-To: <000001c8c727$4b476040$d00f7ca5@lurie.northwestern.edu> Message-ID: My husband has a job with a large international corporation. One of his responsibilities is to make sure that third party vendor suppliers overseas do not employ children under the age of 16. A lot of these factories employ peasants from farms who provide support to the entire farm family. They want the jobs, but this leaves them open to abuse from Also, the working conditions have to meet US standards. By today's standards, children are not allowed to with or around hazardous chemicals. Doesn't matter what state you are in - the federal laws pretty much cover it. On the other side of the coin, when my own kids were younger, I would bring them to work with me on Saturdays where they would help me change the processor. Yeah, but knowing now what I didn't know then - I won't even let my granddaughter in my lab. I even change my clothes before I go to see her after work. I fear the question, "Mommy, why does Grandma smell like formaldehyde and rats?". She's only one year old, but you have to nip these questions in the bud. Bottom line - why would any parent want their kids exposed to the dangerous chemicals we work with? But, again, I do send two of my daughters to work every day to interact with possibly rabid animals. As you can tell, I'm undecided on this topic. "Bernice Frederick" Sent by: histonet-bounces@lists.utsouthwestern.edu 06/05/2008 11:14 AM To "'Rene J Buesa'" , "'Karla Arrington'" , cc Subject RE: [Histonet] Legality of work Not to mention that 15 year olds would not even have the education to do the on-line program. There are requirements as we all know. Scary, scary ,scary. Does this pathologist know the meaning of malpractice and wouldn't the AMA be of help in this as well as ASCP? Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, June 05, 2008 7:51 AM To: Karla Arrington; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Legality of work Here is how I see your problem: 1- you have already contacted the Child and Labor Dept. about this issue, so it is very likely (if they are doing their job) that the will "pay a visit" to the lab and talk with the pathologist. 2- if that is what is going to happen you are going to be in trouble. 3- as the "youngster's" trainer you are accomplice to the violation, because you know her age and keep training her. 4- if this pathologist is also calling names to others, he is an absolute jerk that thinks is owner, not only of the place, but of the world. 5- I don't think this is the right place to work so sooner or later you should try to find a new place to work. 6- if that is going to be how this whole issue is going to end, I think that you should do what is correct and tell him that you are not willing to participate in violating the laboral laws and stop training his daughter. You will sleep better and perhaps this fellow will realize that he is doing something wrong and that you are not willing to accept the situation. At least that is what I would do. Consult with some close friend or family member and present a formal complaint with Child Labor BEFORE doing #6. If he fires you because of this then you will have PROOF that you complained against him because of a law violation and will be able to claim unemployment, even if he says that you were fired for "work substandard performance", you will have proof that this is not the case, but revenge against you. Good luck! Ren? J. Karla Arrington wrote: Histo's: I have roughly 2 dilemmas. The first is of a legal matter. The Pathologist for a week has had his daughter (15 years old), helping me out in the histology lab. He wants her to get training from me, then do the on-line HT program. She has handled chemicals and reagents. I am very uncomfortable with this. I have called the Child Labor Laws department for our state and it is illegal for a 15 year to be in a laboratory. Needless to say working with blood borne pathogens. My most concern is the following. As her "teacher", can I or other co-workers can be held accountable if this is illegal and is found out? This pathologist mind you is the owner of the business. I am afraid if I say something, I will get fired. Where do I go from here or if there is someone who has a similar circumstance happen to them. The other scenario is that this same Pathologist has called a tech a slandering name, twice. There is no "upper management" to go to since he is the owner of the business. I was wondering if this is considered harassment and can this be used to nullify a contract signed by both parties for employment. Concerned, freckles9660@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mickie25 <@t> netzero.net Thu Jun 5 12:40:15 2008 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Thu Jun 5 12:40:23 2008 Subject: [Histonet] Legality of work In-Reply-To: References: <000001c8c727$4b476040$d00f7ca5@lurie.northwestern.edu> Message-ID: Hi All, I have to chime in here. I started in histology by volunteering in high school in my last semester. My 'job' was to change the lids on 8000 pint and quart mason jars of autopsy tissue. This required ripping off the old, corroded lid (if it wouldn't screw off) with a pair of pliers, fill with fresh formalin and screw on a new plastic lid. I did one or two cart loads per day. It took a long time, even beyond the semester. I was hired that summer as a Lab Aid. Needless to say, I was exposed to many things with that job and other duties when I was hired. That was in 1965! And I am still going strong in a field I love, especially since I have added Mohs to my repertoire! That being said, I think with some caution, bringing adolescent children (old enough to follow directions) into the lab for short visits to see what Mom or Dad do for a living can be a wonderful experience and can set some kids on a life-long journey of discovery, even if they don't end up an Histotechnologist. Especially if you can show them some interesting 'stuff' while they are there. I think I would get the approval of the 'boss' whoever that might be and exercising some precautions about exposure to harmful chemicals and sharp instruments, infectious agents, etc. Don't most hospitals have a come to work with parents day still? Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Thursday, June 05, 2008 10:20 AM To: Bernice Frederick Cc: histonet@lists.utsouthwestern.edu; 'Karla Arrington'; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Legality of work My husband has a job with a large international corporation. One of his responsibilities is to make sure that third party vendor suppliers overseas do not employ children under the age of 16. A lot of these factories employ peasants from farms who provide support to the entire farm family. They want the jobs, but this leaves them open to abuse from Also, the working conditions have to meet US standards. By today's standards, children are not allowed to with or around hazardous chemicals. Doesn't matter what state you are in - the federal laws pretty much cover it. On the other side of the coin, when my own kids were younger, I would bring them to work with me on Saturdays where they would help me change the processor. Yeah, but knowing now what I didn't know then - I won't even let my granddaughter in my lab. I even change my clothes before I go to see her after work. I fear the question, "Mommy, why does Grandma smell like formaldehyde and rats?". She's only one year old, but you have to nip these questions in the bud. Bottom line - why would any parent want their kids exposed to the dangerous chemicals we work with? But, again, I do send two of my daughters to work every day to interact with possibly rabid animals. As you can tell, I'm undecided on this topic. "Bernice Frederick" Sent by: histonet-bounces@lists.utsouthwestern.edu 06/05/2008 11:14 AM To "'Rene J Buesa'" , "'Karla Arrington'" , cc Subject RE: [Histonet] Legality of work Not to mention that 15 year olds would not even have the education to do the on-line program. There are requirements as we all know. Scary, scary ,scary. Does this pathologist know the meaning of malpractice and wouldn't the AMA be of help in this as well as ASCP? Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, June 05, 2008 7:51 AM To: Karla Arrington; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Legality of work Here is how I see your problem: 1- you have already contacted the Child and Labor Dept. about this issue, so it is very likely (if they are doing their job) that the will "pay a visit" to the lab and talk with the pathologist. 2- if that is what is going to happen you are going to be in trouble. 3- as the "youngster's" trainer you are accomplice to the violation, because you know her age and keep training her. 4- if this pathologist is also calling names to others, he is an absolute jerk that thinks is owner, not only of the place, but of the world. 5- I don't think this is the right place to work so sooner or later you should try to find a new place to work. 6- if that is going to be how this whole issue is going to end, I think that you should do what is correct and tell him that you are not willing to participate in violating the laboral laws and stop training his daughter. You will sleep better and perhaps this fellow will realize that he is doing something wrong and that you are not willing to accept the situation. At least that is what I would do. Consult with some close friend or family member and present a formal complaint with Child Labor BEFORE doing #6. If he fires you because of this then you will have PROOF that you complained against him because of a law violation and will be able to claim unemployment, even if he says that you were fired for "work substandard performance", you will have proof that this is not the case, but revenge against you. Good luck! Ren? J. Karla Arrington wrote: Histo's: I have roughly 2 dilemmas. The first is of a legal matter. The Pathologist for a week has had his daughter (15 years old), helping me out in the histology lab. He wants her to get training from me, then do the on-line HT program. She has handled chemicals and reagents. I am very uncomfortable with this. I have called the Child Labor Laws department for our state and it is illegal for a 15 year to be in a laboratory. Needless to say working with blood borne pathogens. My most concern is the following. As her "teacher", can I or other co-workers can be held accountable if this is illegal and is found out? This pathologist mind you is the owner of the business. I am afraid if I say something, I will get fired. Where do I go from here or if there is someone who has a similar circumstance happen to them. The other scenario is that this same Pathologist has called a tech a slandering name, twice. There is no "upper management" to go to since he is the owner of the business. I was wondering if this is considered harassment and can this be used to nullify a contract signed by both parties for employment. Concerned, freckles9660@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From viorica.lascau <@t> polymtl.ca Thu Jun 5 12:48:30 2008 From: viorica.lascau <@t> polymtl.ca (Viorica Lascau) Date: Thu Jun 5 12:48:35 2008 Subject: [Histonet] cryogen tape system Message-ID: <484826EE.9090800@polymtl.ca> Hi everyone, I am wondering if somebody is using window tape system and adhesive coated slides (from Instrumedics Inc) for osteochondral cryosections and how you get ride of the stick which remains on the sections (as an opaque spot) before doing immunohistochemistry? I tried acetone for 5 min but it did not work. I guess some stick from window tape remain on tissue as a thin layer which does not allow the antibody to rich the epitopes. I think this problem came from the tape because when I did immunohistochemistry ( ex.collagen II ) on cryosections without tape, the staining was perfect, but unfortunately the sections do not remain on the slides or the bone marrow go a way if I do not use tape Need suggestions, help Thanks a lot Vicky From ardavis <@t> wlgore.com Thu Jun 5 12:50:42 2008 From: ardavis <@t> wlgore.com (Araceli Davis) Date: Thu Jun 5 12:50:54 2008 Subject: [Histonet] LIS Message-ID: Hi, we are looking for pathology software for a small research lab. Does any one have any recommendations? Araceli From CIngles <@t> uwhealth.org Thu Jun 5 13:01:39 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Thu Jun 5 13:01:47 2008 Subject: [Histonet] HT practical References: <005b01c8c694$bf546de0$0302a8c0@yourxhtr8hvc4p><000101c8c70c$c27372a0$d00f7ca5@lurie.northwestern.edu> <1CE1847DFEA0A647B1CCDE4108EA60A7F23AB8@LTA3VS011.ees.hhs.gov> Message-ID: <08A0A863637F1349BBFD83A96B27A50A12013C@uwhis-xchng3.uwhis.hosp.wisc.edu> I for one did EVERYTHING myself for my practical. Including grossing, embedding, etc. That was standard at my internship site that the internist couldn't get any direct help from staff with the practical, and I had to do a MGP! Talk about fun trying to get ahold of the powder to make the solution. I have also come across a former co-worker trying to pass his practical (3rd time around) and wanted whoever was doing specials to "throw his in with the batch". I refused. (mostly because I knew this guy was a bit incompitent.) But I also believe it to be the same as copying someone's answers off a test. If a tech can't do a relatively simple stain or is too lazy to do the work they don't belong in the field. Is it Friday yet? Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thu 6/5/2008 8:27 AM To: Bernice Frederick; Joe Nocito; Martin, Erin; histonet Subject: RE: [Histonet] HT practical I was also told that it was too easy to get someone else to cut and stain your sections. From rfields <@t> gidocs.net Thu Jun 5 13:10:03 2008 From: rfields <@t> gidocs.net (Rosa Fields) Date: Thu Jun 5 13:10:07 2008 Subject: [Histonet] LIS In-Reply-To: References: Message-ID: <2F2611250DCD6549AA3D96CE8AF1F01801123D7F@giexchange.gidocs.net> We really like our new software: AP Easy, they have been a wonderful company to work with! Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Araceli Davis Sent: Thursday, June 05, 2008 12:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] LIS Hi, we are looking for pathology software for a small research lab. Does any one have any recommendations? Araceli _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Thu Jun 5 13:12:38 2008 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Thu Jun 5 13:11:12 2008 Subject: [Histonet] Legality of work In-Reply-To: References: <000001c8c727$4b476040$d00f7ca5@lurie.northwestern.edu> Message-ID: Jackie, Can you point me to the federal regulations you referred to that specify that individuals of a certain age are restricted from working in an environment where hazardous chemicals are used? This information would be beneficial to me. I've enjoyed the interesting discussion stimulated by the original poster. In my mind, though, I have failed to see the "legal" issue the original poster referred to. Both the employer and parental guardian of the adolescent (pathologist/lab owner/parent) consented to the teen working in the laboratory environment. As a board certified physician he is certainly qualified to assess and understand the magnitude of any potential risks. Since we are not familiar with the layout of the lab and the specific activities the teen was engaged in, it is difficult for us to judge to what extent the adolescent is at risk. The statement that the youngster "has handled chemicals and reagents" is too general for me to assess what the youngster is exposed to. are we talking about hematoxylin stain solution, or DAB? Formaldehyde or ethanol? While employment regulations may vary from state to state, in my limited experience, I have witnessed provisions for teens to work part time jobs, after school/weekends and summer employment. My own daughters have had jobs in food establishments at age 15. so it is doubtful that it is illegal for an individual this age to be employed provided the state's requirements are met. And like others, I too exposed my children to the lab in the hope that they might find their own passions, or at the least, learn that the lab was not for them. It seems to me that the original poster has a unique and wonderful opportunity to stimulate the passions of a young person that cannot as effectively be accomplished in a simulated environment outside the laboratory or at a high school career day. Looking beyond that, it is quite possible that the teen will share her experiences with friends/peers who will indirectly learn about histology and some may even be inclined to explore further as they deliberate about career options. Likewise, the poster had, through the teen's experiences, the opportunity to make a great impression on her boss/employer which may have gone a long way to repair what sounds like a tenuous relationship based upon her description of the work environment. It isn't clear that she considered all sides of this issue before contacting the authorities which is most likely to have a destructive result. That aside, we have very limited opportunities to share our passions with young people in ways that may stimulate their curiosities. I suspect that this was the intention of a well meaning father who wanted to share his passions with his daughter. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Thursday, June 05, 2008 1:20 PM To: Bernice Frederick Cc: histonet@lists.utsouthwestern.edu; 'Karla Arrington'; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Legality of work My husband has a job with a large international corporation. One of his responsibilities is to make sure that third party vendor suppliers overseas do not employ children under the age of 16. A lot of these factories employ peasants from farms who provide support to the entire farm family. They want the jobs, but this leaves them open to abuse from Also, the working conditions have to meet US standards. By today's standards, children are not allowed to with or around hazardous chemicals. Doesn't matter what state you are in - the federal laws pretty much cover it. On the other side of the coin, when my own kids were younger, I would bring them to work with me on Saturdays where they would help me change the processor. Yeah, but knowing now what I didn't know then - I won't even let my granddaughter in my lab. I even change my clothes before I go to see her after work. I fear the question, "Mommy, why does Grandma smell like formaldehyde and rats?". She's only one year old, but you have to nip these questions in the bud. Bottom line - why would any parent want their kids exposed to the dangerous chemicals we work with? But, again, I do send two of my daughters to work every day to interact with possibly rabid animals. As you can tell, I'm undecided on this topic. "Bernice Frederick" Sent by: histonet-bounces@lists.utsouthwestern.edu 06/05/2008 11:14 AM To "'Rene J Buesa'" , "'Karla Arrington'" , cc Subject RE: [Histonet] Legality of work Not to mention that 15 year olds would not even have the education to do the on-line program. There are requirements as we all know. Scary, scary ,scary. Does this pathologist know the meaning of malpractice and wouldn't the AMA be of help in this as well as ASCP? Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, June 05, 2008 7:51 AM To: Karla Arrington; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Legality of work Here is how I see your problem: 1- you have already contacted the Child and Labor Dept. about this issue, so it is very likely (if they are doing their job) that the will "pay a visit" to the lab and talk with the pathologist. 2- if that is what is going to happen you are going to be in trouble. 3- as the "youngster's" trainer you are accomplice to the violation, because you know her age and keep training her. 4- if this pathologist is also calling names to others, he is an absolute jerk that thinks is owner, not only of the place, but of the world. 5- I don't think this is the right place to work so sooner or later you should try to find a new place to work. 6- if that is going to be how this whole issue is going to end, I think that you should do what is correct and tell him that you are not willing to participate in violating the laboral laws and stop training his daughter. You will sleep better and perhaps this fellow will realize that he is doing something wrong and that you are not willing to accept the situation. At least that is what I would do. Consult with some close friend or family member and present a formal complaint with Child Labor BEFORE doing #6. If he fires you because of this then you will have PROOF that you complained against him because of a law violation and will be able to claim unemployment, even if he says that you were fired for "work substandard performance", you will have proof that this is not the case, but revenge against you. Good luck! Ren? J. Karla Arrington wrote: Histo's: I have roughly 2 dilemmas. The first is of a legal matter. The Pathologist for a week has had his daughter (15 years old), helping me out in the histology lab. He wants her to get training from me, then do the on-line HT program. She has handled chemicals and reagents. I am very uncomfortable with this. I have called the Child Labor Laws department for our state and it is illegal for a 15 year to be in a laboratory. Needless to say working with blood borne pathogens. My most concern is the following. As her "teacher", can I or other co-workers can be held accountable if this is illegal and is found out? This pathologist mind you is the owner of the business. I am afraid if I say something, I will get fired. Where do I go from here or if there is someone who has a similar circumstance happen to them. The other scenario is that this same Pathologist has called a tech a slandering name, twice. There is no "upper management" to go to since he is the owner of the business. I was wondering if this is considered harassment and can this be used to nullify a contract signed by both parties for employment. Concerned, freckles9660@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JBower <@t> hei.org Thu Jun 5 13:29:37 2008 From: JBower <@t> hei.org (Bower, Jennifer) Date: Thu Jun 5 13:29:45 2008 Subject: [Histonet] Legality of work In-Reply-To: References: <000001c8c727$4b476040$d00f7ca5@lurie.northwestern.edu> Message-ID: <87449E4A2B01DA47B29424CE5D6E0F8308AAF8AB@hi0sml1.hei.org> OK, Here's my 2 cents: I began working when I was 15 and 1/2 at a video store. In California, you need to have a work permit and be 15 and 1/2 to work. There are restrictions about how many hours and on what days you can work. We also had to attend a class on work experience. You would not believe the stories we would hear in class. Managers asking kids to cut class to work, kids not getting paid for work, sexual harassment, all kinds of stuff. For the most part, I think kids aim to please and don't yet have the strength to say no to someone in authority (ie; the person who signs their paycheck) if it means losing the job or maybe deciding to quit for their own safety. I had a job at a vet clinic where part of my job was to go through the sharps container and clean out any non-sharp garbage that got thrown in there by mistake. If someone told me to do that today I would laugh in his face. Children should not work for a reason. Observing in a lab with parental consent is great. But keep them away from the xylene and formaldehyde. Jennifer -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Thursday, June 05, 2008 11:13 AM To: 'Jackie M O'Connor'; Bernice Frederick Cc: histonet@lists.utsouthwestern.edu; 'Karla Arrington'; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Legality of work Jackie, Can you point me to the federal regulations you referred to that specify that individuals of a certain age are restricted from working in an environment where hazardous chemicals are used? This information would be beneficial to me. I've enjoyed the interesting discussion stimulated by the original poster. In my mind, though, I have failed to see the "legal" issue the original poster referred to. Both the employer and parental guardian of the adolescent (pathologist/lab owner/parent) consented to the teen working in the laboratory environment. As a board certified physician he is certainly qualified to assess and understand the magnitude of any potential risks. Since we are not familiar with the layout of the lab and the specific activities the teen was engaged in, it is difficult for us to judge to what extent the adolescent is at risk. The statement that the youngster "has handled chemicals and reagents" is too general for me to assess what the youngster is exposed to. are we talking about hematoxylin stain solution, or DAB? Formaldehyde or ethanol? While employment regulations may vary from state to state, in my limited experience, I have witnessed provisions for teens to work part time jobs, after school/weekends and summer employment. My own daughters have had jobs in food establishments at age 15. so it is doubtful that it is illegal for an individual this age to be employed provided the state's requirements are met. And like others, I too exposed my children to the lab in the hope that they might find their own passions, or at the least, learn that the lab was not for them. It seems to me that the original poster has a unique and wonderful opportunity to stimulate the passions of a young person that cannot as effectively be accomplished in a simulated environment outside the laboratory or at a high school career day. Looking beyond that, it is quite possible that the teen will share her experiences with friends/peers who will indirectly learn about histology and some may even be inclined to explore further as they deliberate about career options. Likewise, the poster had, through the teen's experiences, the opportunity to make a great impression on her boss/employer which may have gone a long way to repair what sounds like a tenuous relationship based upon her description of the work environment. It isn't clear that she considered all sides of this issue before contacting the authorities which is most likely to have a destructive result. That aside, we have very limited opportunities to share our passions with young people in ways that may stimulate their curiosities. I suspect that this was the intention of a well meaning father who wanted to share his passions with his daughter. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Thursday, June 05, 2008 1:20 PM To: Bernice Frederick Cc: histonet@lists.utsouthwestern.edu; 'Karla Arrington'; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Legality of work My husband has a job with a large international corporation. One of his responsibilities is to make sure that third party vendor suppliers overseas do not employ children under the age of 16. A lot of these factories employ peasants from farms who provide support to the entire farm family. They want the jobs, but this leaves them open to abuse from Also, the working conditions have to meet US standards. By today's standards, children are not allowed to with or around hazardous chemicals. Doesn't matter what state you are in - the federal laws pretty much cover it. On the other side of the coin, when my own kids were younger, I would bring them to work with me on Saturdays where they would help me change the processor. Yeah, but knowing now what I didn't know then - I won't even let my granddaughter in my lab. I even change my clothes before I go to see her after work. I fear the question, "Mommy, why does Grandma smell like formaldehyde and rats?". She's only one year old, but you have to nip these questions in the bud. Bottom line - why would any parent want their kids exposed to the dangerous chemicals we work with? But, again, I do send two of my daughters to work every day to interact with possibly rabid animals. As you can tell, I'm undecided on this topic. "Bernice Frederick" Sent by: histonet-bounces@lists.utsouthwestern.edu 06/05/2008 11:14 AM To "'Rene J Buesa'" , "'Karla Arrington'" , cc Subject RE: [Histonet] Legality of work Not to mention that 15 year olds would not even have the education to do the on-line program. There are requirements as we all know. Scary, scary ,scary. Does this pathologist know the meaning of malpractice and wouldn't the AMA be of help in this as well as ASCP? Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, June 05, 2008 7:51 AM To: Karla Arrington; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Legality of work Here is how I see your problem: 1- you have already contacted the Child and Labor Dept. about this issue, so it is very likely (if they are doing their job) that the will "pay a visit" to the lab and talk with the pathologist. 2- if that is what is going to happen you are going to be in trouble. 3- as the "youngster's" trainer you are accomplice to the violation, because you know her age and keep training her. 4- if this pathologist is also calling names to others, he is an absolute jerk that thinks is owner, not only of the place, but of the world. 5- I don't think this is the right place to work so sooner or later you should try to find a new place to work. 6- if that is going to be how this whole issue is going to end, I think that you should do what is correct and tell him that you are not willing to participate in violating the laboral laws and stop training his daughter. You will sleep better and perhaps this fellow will realize that he is doing something wrong and that you are not willing to accept the situation. At least that is what I would do. Consult with some close friend or family member and present a formal complaint with Child Labor BEFORE doing #6. If he fires you because of this then you will have PROOF that you complained against him because of a law violation and will be able to claim unemployment, even if he says that you were fired for "work substandard performance", you will have proof that this is not the case, but revenge against you. Good luck! Ren? J. Karla Arrington wrote: Histo's: I have roughly 2 dilemmas. The first is of a legal matter. The Pathologist for a week has had his daughter (15 years old), helping me out in the histology lab. He wants her to get training from me, then do the on-line HT program. She has handled chemicals and reagents. I am very uncomfortable with this. I have called the Child Labor Laws department for our state and it is illegal for a 15 year to be in a laboratory. Needless to say working with blood borne pathogens. My most concern is the following. As her "teacher", can I or other co-workers can be held accountable if this is illegal and is found out? This pathologist mind you is the owner of the business. I am afraid if I say something, I will get fired. Where do I go from here or if there is someone who has a similar circumstance happen to them. The other scenario is that this same Pathologist has called a tech a slandering name, twice. There is no "upper management" to go to since he is the owner of the business. I was wondering if this is considered harassment and can this be used to nullify a contract signed by both parties for employment. Concerned, freckles9660@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alonso.martinezcanabal <@t> utoronto.ca Thu Jun 5 13:30:44 2008 From: alonso.martinezcanabal <@t> utoronto.ca (Alonso Martinez-Canabal) Date: Thu Jun 5 13:31:06 2008 Subject: [Histonet] Vibratome mounted sections falling, Message-ID: Hi, I need desperate help. I cut and mounted 40microns brain section using a vibratome on poly-l-lysine slides, a lot of them. Once in a while a try to do immunohistochemistry and was ok. So, for some reason, now, time to make the big experiment the sections are falling of in the antigen retrieval procedured (citrate buffer, boiling water). I have tried to dry them in alcohols, in the oven, fixation again, all has been useless, and without the AR i do not have the quality of staining that I (and my proud boss) observed before. Please, some help here Thanks. Alonso From Sandra.Harrison3 <@t> va.gov Thu Jun 5 13:32:47 2008 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Thu Jun 5 13:32:51 2008 Subject: [Histonet] Feedback on tissue arrayer, please? Message-ID: <> Dear Histonetters, If any of you have tried this tissue arrayer, could you please share your impressions? The video describing the use of this product is only 3-5 minutes. It looks very impressive! Shortcut to: http://arraymold.com/order.html From Jackie.O'Connor <@t> abbott.com Thu Jun 5 13:35:21 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Jun 5 13:35:38 2008 Subject: [Histonet] Legality of work In-Reply-To: Message-ID: I doublechecked my information with my spouse. I was misinformed and spread it to you guys. There is no US law governing children and hazardous chemicals - but there is a law in China. So, don't let your kids go to China to work in factories. Sorry about the error. There was no sniper fire afterall. If you can forgive her, you can forgive me. From b-frederick <@t> northwestern.edu Thu Jun 5 13:52:55 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Jun 5 13:53:07 2008 Subject: [Histonet] HT practical In-Reply-To: <08A0A863637F1349BBFD83A96B27A50A12013C@uwhis-xchng3.uwhis.hosp.wisc.edu> Message-ID: <000101c8c73d$60c27bb0$d00f7ca5@lurie.northwestern.edu> Than you Claire for taking the words out of my mouth. I feel the same way. At least we had the MGP powder! (Now Richard Allen sells the liquid stain) Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Thursday, June 05, 2008 1:02 PM To: histonet Subject: RE: [Histonet] HT practical I for one did EVERYTHING myself for my practical. Including grossing, embedding, etc. That was standard at my internship site that the internist couldn't get any direct help from staff with the practical, and I had to do a MGP! Talk about fun trying to get ahold of the powder to make the solution. I have also come across a former co-worker trying to pass his practical (3rd time around) and wanted whoever was doing specials to "throw his in with the batch". I refused. (mostly because I knew this guy was a bit incompitent.) But I also believe it to be the same as copying someone's answers off a test. If a tech can't do a relatively simple stain or is too lazy to do the work they don't belong in the field. Is it Friday yet? Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thu 6/5/2008 8:27 AM To: Bernice Frederick; Joe Nocito; Martin, Erin; histonet Subject: RE: [Histonet] HT practical I was also told that it was too easy to get someone else to cut and stain your sections. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Madeline.Branflick <@t> sanofi-aventis.com Thu Jun 5 14:12:10 2008 From: Madeline.Branflick <@t> sanofi-aventis.com (Madeline.Branflick@sanofi-aventis.com) Date: Thu Jun 5 14:12:21 2008 Subject: [Histonet] Pancreas RNA Message-ID: Hello: Does anyone have experience working with pancreas tissue for downstream LCM? Particularly FFPE and frozen collection procedures/protocols. We have tried both formats, and were unsuccessful in maintaining RNA integrity. We've tried to get around the endogenous ribonuclease issue by snap freezing in chilled isopentane, or a direct fixation in 10% NBF. Any help is greatly appreciated. Madeline From POWELL_SA <@t> Mercer.edu Thu Jun 5 14:48:18 2008 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Thu Jun 5 14:53:26 2008 Subject: [Histonet] Need Help from Mexico Message-ID: <01MVMZ5O37YS006XTJ@Macon2.Mercer.edu> Hi histonetters, One of my pathologists here at Mercer is presently preparing to go on a medical mission trip to Mexico with a group of our medical students. She has a somewhat old but beloved AO microscope (model is an 1130A) to take with her. The bulb is an Osram halogen xenophot with the letters/numbers on it - HLX 64250 ESB 6V 20W. There is no switch to select voltages. Do you have a recommendation for any required adapter, she was told they will have power (possibly from a generator) where the clinic will be located but does not know what voltage to expect. Any help will be appreciated. I know nothing of electricity requirements in other countries and we are trying to get all that they will need while there. Thanks! Shirley Powell Mercer University School of Medicine Technical Director Histology Curricular Support Lab 1550 College Street Macon, GA 31207 478-301-2374 From detmar <@t> mshri.on.ca Thu Jun 5 14:58:13 2008 From: detmar <@t> mshri.on.ca (Jacqui Detmar) Date: Thu Jun 5 14:58:22 2008 Subject: [Histonet] Pancreas RNA In-Reply-To: References: Message-ID: Hey there. I *personally* haven't done any RNA work for LCM on pancreatic tissue, but I have listened to two (independent) researchers - who work on mouse pancreas - rave about how RNAlater (Ambion) saved their sanity. Another researcher really liked the LCM staining kit (also from Ambion) b/c it prevented the activation of endogenous proteases through the use of non-aqueous solutions. Again, I haven't used these techniques, but I've heard how difficult the pancreas can be to deal with and I thought I would pass these on as a possibility. Good luck, Jacqui Jacqui Detmar, Post-doctoral Fellow Samuel Lunenfeld Research Institute, room 876 Mount Sinai Hospital 600 University Avenue Toronto, ON, Canada M5G 1X5 phone: 416-586-4800 x2451/x2290 fax: 416-586-8588 email: detmar@mshri.on.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Madeline.Branflick@sanofi-aventis.com Sent: Thursday, June 05, 2008 3:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pancreas RNA Hello: Does anyone have experience working with pancreas tissue for downstream LCM? Particularly FFPE and frozen collection procedures/protocols. We have tried both formats, and were unsuccessful in maintaining RNA integrity. We've tried to get around the endogenous ribonuclease issue by snap freezing in chilled isopentane, or a direct fixation in 10% NBF. Any help is greatly appreciated. Madeline _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From freckles9660 <@t> yahoo.com Thu Jun 5 15:07:20 2008 From: freckles9660 <@t> yahoo.com (Karla Arrington) Date: Thu Jun 5 15:07:26 2008 Subject: [Histonet] Clarification of Legality of work Message-ID: <613914.34749.qm@web32501.mail.mud.yahoo.com> Fellow Histo's: ? Thanks for the many responses. But I feel that I have to clarify the situation.? In this state, HT's are very hard to come by. So with that in mind, the Pathologist suggested his 15 year old daughter to help me, working summers and holidays.??The plan is for me to train her as a Histology student taking their clinical.? Also, the Pathologist wants her to take the on-line HT program to get certified.? Do you not have to have a high school diploma first? She is only 15!! ?She is also to be paid by the companies payroll.??She has not been exposed to any of the basics.??So therefore, I am?"teaching" a minor?every aspect of Histology. When I need time off, she will be responsible for the work that is performed.? This includes embedding, microtomy, staining, special stains, IHC's, maintenance; etc.? She would be a 15 year old doing the work of a certified HT.? To this I feel very uncomfortable.??In response, I called our state Child Labor law department and they said that? it is ILLEGAL for a minor, under the age of 17 to be working in the medical laboratory, especially with blood borne pathogens.? When she is here, I am solely responsible for her as her parent is usually unavailable, so therefore no direct supervision by the Pathologist.??If "caught", I don't want to?loose my HT license because of this. If I disagree to not "teach" his daughter, I?know?there will be consequences for me.? And that leads to the?second dilemma. Any suggestion? ? Concerned, freckles9660@yahoo.com From JMcCormick <@t> schosp.org Thu Jun 5 15:51:45 2008 From: JMcCormick <@t> schosp.org (McCormick, James) Date: Thu Jun 5 15:51:52 2008 Subject: [Histonet] Clarification of Legality of work In-Reply-To: <613914.34749.qm@web32501.mail.mud.yahoo.com> References: <613914.34749.qm@web32501.mail.mud.yahoo.com> Message-ID: <95D447442469F44DB2A370783169A3E804DB2D5A@exch01.backup> Second response: from "an interested pathologist" When I was a young man, I too was called "freckles ! Why not take all of the information that you have gathered to your pathologist/boss with the concerns expressed. Ask him to clear the road by HIS formal employment of his daughter and when properly employed (at his risk) bring her into the lab as an apprentice. In this role she should have the responsibility ( on work time) to WRITE weekly reports on her work. I have a hunch that this routine might be too rigorous for her to last. On the other hand, if she flourishes with success in an agenda of progression that has been assigned to her apprentice role....you may have contributed to motivating another quality person to our profession. Will she resume and complete her formal schooling in the fall???..........good idea ! Good luck, J.B.McCormick, M.D, -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Karla Arrington Sent: Thursday, June 05, 2008 3:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Clarification of Legality of work Fellow Histo's: ? Thanks for the many responses. But I feel that I have to clarify the situation.? In this state, HT's are very hard to come by. So with that in mind, the Pathologist suggested his 15 year old daughter to help me, working summers and holidays.??The plan is for me to train her as a Histology student taking their clinical.? Also, the Pathologist wants her to take the on-line HT program to get certified.? Do you not have to have a high school diploma first? She is only 15!! ?She is also to be paid by the companies payroll.??She has not been exposed to any of the basics.??So therefore, I am?"teaching" a minor?every aspect of Histology. When I need time off, she will be responsible for the work that is performed.? This includes embedding, microtomy, staining, special stains, IHC's, maintenance; etc.? She would be a 15 year old doing the work of a certified HT.? To this I feel very uncomfortable.??In response, I called our state Child Labor law department and they said that? it is ILLEGAL for a minor, under the age of 17 to be working in the medical laboratory, especially with blood borne pathogens.? When she is here, I am solely responsible for her as her parent is usually unavailable, so therefore no direct supervision by the Pathologist.??If "caught", I don't want to?loose my HT license because of this. If I disagree to not "teach" his daughter, I?know?there will be consequences for me.? And that leads to the?second dilemma. Any suggestion? ? Concerned, freckles9660@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From joelleweaver <@t> hotmail.com Thu Jun 5 16:12:17 2008 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Jun 5 16:12:23 2008 Subject: [Histonet] Clarification of Legality of work In-Reply-To: <95D447442469F44DB2A370783169A3E804DB2D5A@exch01.backup> References: <613914.34749.qm@web32501.mail.mud.yahoo.com> <95D447442469F44DB2A370783169A3E804DB2D5A@exch01.backup> Message-ID: I just wanted to say that the suggestion below seems to be a good one. I think that it is good practice to formally bring your concerns and complaints directly to the person who may attend to them. That being said, tact and diplomacy can help here. If you get a bad response, you have convered yourself and given them the opportunity to resond, correct and explain. But, I do think that the suggestion to push the formal act of officially hiring a minor does place the responsibility directly on the party employing her with regards to her rights to safety, appropriate PPE, and training -that other "offical" employees enjoy. This may resolve the situation. But it sounds to me that there are other interpersonal issues as well? However, clearing one issue can only serve to lessen the stress and tension. Hope this helps? Joelle Weaver BA, HTL (ASCP)> Date: Thu, 5 Jun 2008 15:51:45 -0500> From: JMcCormick@schosp.org> To: freckles9660@yahoo.com; histonet@lists.utsouthwestern.edu> Subject: RE: [Histonet] Clarification of Legality of work> CC: > > Second response: from "an interested pathologist" > When I was a young man, I too was called "freckles !> > Why not take all of the information that you have gathered to your pathologist/boss with the concerns expressed. > Ask him to clear the road by HIS formal employment of his daughter and when properly employed (at his risk) bring her into the lab as an apprentice. In this role she should have the responsibility ( on work time) to WRITE weekly reports on her work. I have a hunch that this routine might be too rigorous for her to last. On the other hand, if she flourishes with success in an agenda of progression that has been assigned to her apprentice role....you may have contributed to motivating another quality person to our profession. Will she resume and complete her formal schooling in the fall???..........good idea ! > > Good luck,> J.B.McCormick, M.D,> > > > > -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Karla Arrington> Sent: Thursday, June 05, 2008 3:07 PM> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] Clarification of Legality of work> > Fellow Histo's:> > Thanks for the many responses. But I feel that I have to clarify the situation. In this state, HT's are very hard to come by. So with that in mind, the Pathologist suggested his 15 year old daughter to help me, working summers and holidays. The plan is for me to train her as a Histology student taking their clinical. Also, the Pathologist wants her to take the on-line HT program to get certified. Do you not have to have a high school diploma first? She is only 15!! She is also to be paid by the companies payroll. She has not been exposed to any of the basics. So therefore, I am "teaching" a minor every aspect of Histology. When I need time off, she will be responsible for the work that is performed. This includes embedding, microtomy, staining, special stains, IHC's, maintenance; etc. She would be a 15 year old doing the work of a certified HT. To this I feel very uncomfortable. In response, I called our state Child Labor law> department and they said that it is ILLEGAL for a minor, under the age of 17 to be working in the medical laboratory, especially with blood borne pathogens. When she is here, I am solely responsible for her as her parent is usually unavailable, so therefore no direct supervision by the Pathologist. If "caught", I don't want to loose my HT license because of this. If I disagree> to not "teach" his daughter, I know there will be consequences for me. And that leads to the second dilemma.> Any suggestion?> > Concerned, > freckles9660@yahoo.com> > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > *** Confidentiality Statement ***> This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction.> > > Thank you for your cooperation.> > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Search that pays you back! Introducing Live Search cashback. http://search.live.com/cashback/?&pkw=form=MIJAAF/publ=HMTGL/crea=srchpaysyouback From joelleweaver <@t> hotmail.com Thu Jun 5 16:19:00 2008 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Jun 5 16:19:04 2008 Subject: [Histonet] fed. regulations child labor Message-ID: Hi all http://www.dol.gov/dol/topic/youthlabor/agerequirements.htm#lawregs Fair Labor Standards Act (FLSA)-The rules vary depending upon the particular age of the minor and the particular job involved. As a general rule, the FLSA sets 14 years of age as the minimum age for employment, and limits the number of hours worked by minors under the age of 16. Also, the FLSA generally prohibits the employment of a minor in work declared hazardous by the Secretary of Labor (for example, work involving excavation, driving, and the operation of many types of power-driven equipment). The FLSA contains a number of requirements that apply only to particular types of jobs (for example, agricultural work or the operation of motor vehicles) and many exceptions to the general rules (for example, work by a minor for his or her parents). Each state also has its own laws relating to employment, including the employment of minors. If state law and the FLSA overlap, the law which is more protective of the minor will apply. The FLSA "covers" or applies to all employees of certain "enterprises." All employees of an enterprise, as defined by the FLSA, are covered regardless of the duties they perform. If a worker is not an employee of one of these enterprises, he or she may still be covered if the employee's own duties meet certain interstate commerce requirements "enterprise" inlcudes: A hospital, or an institution primarily engaged in the care of the sick, the aged, or the mentally ill or mentally retarded who live on the premises (it does not matter if the hospital or institution is public or private or is operated for profit or not-for-profit) Regulations29 CFR ?570.2 Minimum Age Standards for Nonagricultural Employment 29 CFR ??570.31-34 Thanks, Joelle Weaver BA, HTL(ASCP) _________________________________________________________________ It?s easy to add contacts from Facebook and other social sites through Windows Live? Messenger. Learn how. https://www.invite2messenger.net/im/?source=TXT_EML_WLH_LearnHow From CBark <@t> memorialcare.org Thu Jun 5 16:30:53 2008 From: CBark <@t> memorialcare.org (Christine Bark) Date: Thu Jun 5 16:31:23 2008 Subject: [Histonet] Decolorize PAS Message-ID: Hello all, My pathologist asked me to de-stain a PAS-stained section of liver and re-stain it with a trichrome. I've never decolorized anything other than an H&E. Would I still use acid alcohol and if so for how long? I've read that potassium metabisulfite will take out excess Schiff's but I've never tried that and I don't have any. Any suggestions/tips? Thank you, Christine Bark HT(ASCP) ______________________________________________________________________________ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From talulahgosh <@t> gmail.com Thu Jun 5 18:39:57 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Jun 5 18:40:02 2008 Subject: [Histonet] LIS In-Reply-To: <2F2611250DCD6549AA3D96CE8AF1F01801123D7F@giexchange.gidocs.net> References: <2F2611250DCD6549AA3D96CE8AF1F01801123D7F@giexchange.gidocs.net> Message-ID: What does one use pathology software for? Just organizing files? Emily -- When you're riding in a time machine way far into the future, don't stick your elbow out the window, or it'll turn into a fossil. From Tony_Reilly <@t> health.qld.gov.au Thu Jun 5 18:56:01 2008 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Thu Jun 5 18:56:22 2008 Subject: [Histonet] question In-Reply-To: <343243.51820.qm@web45110.mail.sp1.yahoo.com> References: <343243.51820.qm@web45110.mail.sp1.yahoo.com> Message-ID: <484909AF.471C.0039.0@health.qld.gov.au> Kathy You have appeared to try every bluing agent possible, however in my experience the most common cause of pale nuclei in frozen sections is inadequate fixation. What are you using? The best I have used is 5% Conc Formalin in 95% Ethanol for at least 30 sec and longer if time permits. All the best. Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_reilly@health.qld.gov.au >>> "Stephen Peters M.D." 6/06/2008 1:34 am >>> Kathy, You may also want to consider the thickness of your sections. A great deal of our information is gatered at scanning magnification of 2x or 4x. If you are cutting at 3 or 4 microns or if your cryostat is offering a variety of thick and thin the slides will be pale at these powers compared to 5 or 6 micron sections. Presence of a lot of nuclear "holes"is a sign it is cut very thin. Also make sure your hematoxalin is changed regularly and is not growing "rock candy" in the bottom. Check slide after it leaves the bluing and get to know the shade of blue that represents a well stained slide. Remember when checking the slides, the amount of blue will depend on the densityof nuclear material as well as the thickness of the tissue. I find the actual shade of blue tells me that it has been stained in hemotoxalin long enough. Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From suboro <@t> yahoo.com Thu Jun 5 19:05:17 2008 From: suboro <@t> yahoo.com (suboro) Date: Thu Jun 5 19:05:20 2008 Subject: [Histonet] electrical adapter for Mexico Message-ID: <81297.32717.qm@web34703.mail.mud.yahoo.com> Shirley, You can find power adapters at travel stores, or even WalMart. The one I took to Honduras had the various countries or regions where it might be applicable listed right on the package. BTW, we were not allowed to carry on our scope. Security at the gate informed us only one carry-on into Honduras was allowed, so we had to make some quick and scary decisions; the poor scope had to go with baggage. Call your airlines to see if Mexico is similar, and at least your scope can be packed appropriately.     Susan Beam me up, Scotty From mohs76009 <@t> yahoo.com Thu Jun 5 19:27:15 2008 From: mohs76009 <@t> yahoo.com (Matt Bancroft) Date: Thu Jun 5 19:27:18 2008 Subject: [Histonet] LIS In-Reply-To: Message-ID: <946183.93981.qm@web63414.mail.re1.yahoo.com> AP Easy   You can look at it at APEASY.com   --- On Thu, 6/5/08, Araceli Davis <ardavis@wlgore.com> wrote: From: Araceli Davis <ardavis@wlgore.com> Subject: [Histonet] LIS To: histonet@lists.utsouthwestern.edu Date: Thursday, June 5, 2008, 12:50 PM Hi, we are looking for pathology software for a small research lab. Does any one have any recommendations? Araceli _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Thu Jun 5 19:42:50 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Jun 5 19:43:01 2008 Subject: [Histonet] question In-Reply-To: <484909AF.471C.0039.0@health.qld.gov.au> Message-ID: I concur (now that's an old word) Or, how about: I am unanimous in this Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anthony Reilly Sent: Friday, 6 June 2008 9:56 AM To: Histonet@lists.utsouthwestern.edu; Stephen Peters M.D. Subject: Re: [Histonet] question Kathy You have appeared to try every bluing agent possible, however in my experience the most common cause of pale nuclei in frozen sections is inadequate fixation. What are you using? The best I have used is 5% Conc Formalin in 95% Ethanol for at least 30 sec and longer if time permits. All the best. Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_reilly@health.qld.gov.au >>> "Stephen Peters M.D." 6/06/2008 1:34 am >>> Kathy, You may also want to consider the thickness of your sections. A great deal of our information is gatered at scanning magnification of 2x or 4x. If you are cutting at 3 or 4 microns or if your cryostat is offering a variety of thick and thin the slides will be pale at these powers compared to 5 or 6 micron sections. Presence of a lot of nuclear "holes"is a sign it is cut very thin. Also make sure your hematoxalin is changed regularly and is not growing "rock candy" in the bottom. Check slide after it leaves the bluing and get to know the shade of blue that represents a well stained slide. Remember when checking the slides, the amount of blue will depend on the densityof nuclear material as well as the thickness of the tissue. I find the actual shade of blue tells me that it has been stained in hemotoxalin long enough. Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************ ******** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ************************************************************************ ********** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From micro <@t> superlink.net Thu Jun 5 21:04:50 2008 From: micro <@t> superlink.net (Markus F. Meyenhofer) Date: Thu Jun 5 21:05:28 2008 Subject: [Histonet] Legality of work References: <163789.93086.qm@web32506.mail.mud.yahoo.com><466600.51945.qm@web65704.mail.ac4.yahoo.com> <95D447442469F44DB2A370783169A3E804DB2667@exch01.backup> Message-ID: <09ac01c8c779$b61922d0$a3893cd1@DJ4VDH31> Thank you! Regards, Markus F. Meyenhofer Microscopy Labs Red Bank, NJ ----- Original Message ----- From: "McCormick, James" To: "Rene J Buesa" ; "Karla Arrington" ; Sent: Thursday, June 05, 2008 9:29 AM Subject: RE: [Histonet] Legality of work >From an interested Pathologist: I think you are building a bomb to attack an mouse! When our son and daughter were 14 and 15 years old I was proud to have them VOLUNTEER (non-paid) service to the our laboratory. First to meet the fine people who worked in the department. Second to learn about "dad's work", and third to develop a sense of appreciation for our work in serving others. The children benefited by the social relationship and grew in responsibility by graduating from tasks of simple "go for's", observers, awarded responsibility. They were not permitted to prepare patient materials but they were, over a period of summer volunteer effort, taught to make a collection of their own slides and even to do a frozen section. The experience is a treasured memory and valued experience for the Pathologist and his family and for the bonding of employees who for that period were extended members of the family. Our son is now completing his residency in orthopaedic Surgery and our daughter is a junior officer in a New York company where she has employees reporting to her service. I continue to communicate with the lab employees and they always ask .....tell us how your son and daughter are doing and send them our love. The well operated pathology lab is no more dangerous than a high school biology and chemistry laboratory. I believe you would do yourself,the lab.,the pathologist and the 15 year old a great service by reexamining the situation in a more generous and understanding way. If you are at odds with the pathologist.... take time to visit with him and structure a helping and learning experience for the young lady who just happens to be your bosses daughter. Ask the young lady to observe and take notes for a week. Chart the path of a patient specimen from the beginning through the final diagnosis. Write (required) a report of the reason the specimen was sent to the lab. The complete process/path of the specimen through the lab,and the final result/diagnosis for the patient's benefit. If the 15 year old does this there will be a change in the climate and "dad" will be amazed at what you contribute to the process of health care and to the maturing of his daughter. Just a few thoughts from a Pathologist and dad with success written in the log of his children and their bonding to the profession. Sincerely, and in the spirit of helping. Please give a copy of this note to your pathologist. Kindest regards, J.B.McCormick, M.D. jmccormi@schosp.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, June 05, 2008 7:51 AM To: Karla Arrington; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Legality of work Here is how I see your problem: 1- you have already contacted the Child and Labor Dept. about this issue, so it is very likely (if they are doing their job) that the will "pay a visit" to the lab and talk with the pathologist. 2- if that is what is going to happen you are going to be in trouble. 3- as the "youngster's" trainer you are accomplice to the violation, because you know her age and keep training her. 4- if this pathologist is also calling names to others, he is an absolute jerk that thinks is owner, not only of the place, but of the world. 5- I don't think this is the right place to work so sooner or later you should try to find a new place to work. 6- if that is going to be how this whole issue is going to end, I think that you should do what is correct and tell him that you are not willing to participate in violating the laboral laws and stop training his daughter. You will sleep better and perhaps this fellow will realize that he is doing something wrong and that you are not willing to accept the situation. At least that is what I would do. Consult with some close friend or family member and present a formal complaint with Child Labor BEFORE doing #6. If he fires you because of this then you will have PROOF that you complained against him because of a law violation and will be able to claim unemployment, even if he says that you were fired for "work substandard performance", you will have proof that this is not the case, but revenge against you. Good luck! Ren? J. Karla Arrington wrote: Histo's: I have roughly 2 dilemmas. The first is of a legal matter. The Pathologist for a week has had his daughter (15 years old), helping me out in the histology lab. He wants her to get training from me, then do the on-line HT program. She has handled chemicals and reagents. I am very uncomfortable with this. I have called the Child Labor Laws department for our state and it is illegal for a 15 year to be in a laboratory. Needless to say working with blood borne pathogens. My most concern is the following. As her "teacher", can I or other co-workers can be held accountable if this is illegal and is found out? This pathologist mind you is the owner of the business. I am afraid if I say something, I will get fired. Where do I go from here or if there is someone who has a similar circumstance happen to them. The other scenario is that this same Pathologist has called a tech a slandering name, twice. There is no "upper management" to go to since he is the owner of the business. I was wondering if this is considered harassment and can this be used to nullify a contract signed by both parties for employment. Concerned, freckles9660@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Fri Jun 6 00:14:12 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Jun 6 00:14:16 2008 Subject: [Histonet] Legality of work In-Reply-To: <163789.93086.qm@web32506.mail.mud.yahoo.com> References: <163789.93086.qm@web32506.mail.mud.yahoo.com> Message-ID: <5b6eb13e0806052214s24f0fd22le367585744814b07@mail.gmail.com> Hi Karla, If it turns out that there is nothing illegal about training her, would you still have a problem doing it? You mentioned that the pathologist has called someone a name. I'm guessing that he might not be well liked. If that's the case, would this be something that his daughter would be likely to notice? One final question, are you the only HT in the lab? On Wed, Jun 4, 2008 at 4:37 PM, Karla Arrington wrote: > Histo's: > > I have roughly 2 dilemmas. The first is of a legal matter. The > Pathologist for a week has had his daughter (15 years old), helping > me out in the histology lab. He wants her to get training from me, then do > the on-line HT program. She has handled chemicals > and reagents. I am very uncomfortable with this. I have called the Child > Labor Laws department for our state and it is illegal > for a 15 year to be in a laboratory. Needless to say working with blood > borne pathogens. My most concern is the following. As her "teacher", > can I or other co-workers can be held accountable if this is illegal and is > found out? This pathologist mind you is the owner of the business. > I am afraid if I say something, I will get fired. Where do I go from here > or if there is someone who has a similar circumstance happen to them. > The other scenario is that this same Pathologist has called a tech a > slandering name, twice. There is no "upper management" to go to > since he is the owner of the business. I was wondering if this is > considered harassment and can this be used to nullify a contract signed > by both parties for employment. > > Concerned, > freckles9660@yahoo.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From talulahgosh <@t> gmail.com Fri Jun 6 02:15:23 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Jun 6 02:15:27 2008 Subject: [Histonet] Legality of work In-Reply-To: <5b6eb13e0806052214s24f0fd22le367585744814b07@mail.gmail.com> References: <163789.93086.qm@web32506.mail.mud.yahoo.com> <5b6eb13e0806052214s24f0fd22le367585744814b07@mail.gmail.com> Message-ID: With no experience outside of the academic lab, let me tell you: break every taboo you can. Especially if it deals with a daughter and father--put her in control (ie she knows what she's doing, or at least you taught her that) and keep the father in it as little as possible. This is what we need in our labs! It takes a small change to create a fantastic lab--I hope you create that, regardless of the company's position in the stock market or expected financial ground. I speak from my idealistic grant world (in which, believe me, they don't afford me anything over 2.5% living wage of 29000, the latest), yet I love my job and wouldn't give it up for the world. (Background: 31 with a BS in microbio, been a technician for 9 years and loving it) Consider that when you get a kid--would they love it as much as a geek like me? I <3 my science job, Emily -- When you're riding in a time machine way far into the future, don't stick your elbow out the window, or it'll turn into a fossil. From rjbuesa <@t> yahoo.com Fri Jun 6 07:56:19 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 6 07:56:23 2008 Subject: [Histonet] Need Help from Mexico In-Reply-To: <01MVMZ5O37YS006XTJ@Macon2.Mercer.edu> Message-ID: <759763.62935.qm@web65713.mail.ac4.yahoo.com> Electrical power in Mexico is the same voltage as in the US, even the electrical outlets are identical. Just returned from Mexico. Ren? J. Shirley Powell wrote: Hi histonetters, One of my pathologists here at Mercer is presently preparing to go on a medical mission trip to Mexico with a group of our medical students. She has a somewhat old but beloved AO microscope (model is an 1130A) to take with her. The bulb is an Osram halogen xenophot with the letters/numbers on it - HLX 64250 ESB 6V 20W. There is no switch to select voltages. Do you have a recommendation for any required adapter, she was told they will have power (possibly from a generator) where the clinic will be located but does not know what voltage to expect. Any help will be appreciated. I know nothing of electricity requirements in other countries and we are trying to get all that they will need while there. Thanks! Shirley Powell Mercer University School of Medicine Technical Director Histology Curricular Support Lab 1550 College Street Macon, GA 31207 478-301-2374 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Jun 6 08:03:46 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 6 08:03:53 2008 Subject: [Histonet] Decolorize PAS In-Reply-To: Message-ID: <811514.42631.qm@web65710.mail.ac4.yahoo.com> PAS is a very strong reaction extremely difficult to destain. Acid treatments that could fade it will affect the reactivity of the tissue for further staining procedures. Ren? J. Christine Bark wrote: Hello all, My pathologist asked me to de-stain a PAS-stained section of liver and re-stain it with a trichrome. I've never decolorized anything other than an H&E. Would I still use acid alcohol and if so for how long? I've read that potassium metabisulfite will take out excess Schiff's but I've never tried that and I don't have any. Any suggestions/tips? Thank you, Christine Bark HT(ASCP) ______________________________________________________________________________ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From beverly <@t> histologytechservices.com Fri Jun 6 09:10:08 2008 From: beverly <@t> histologytechservices.com (Beverly Robinson) Date: Fri Jun 6 09:10:23 2008 Subject: [Histonet] unsubscribe Message-ID: Please remove me / unsubscribe me from the histonet list From GDeMatteo <@t> srhs.org Fri Jun 6 09:22:18 2008 From: GDeMatteo <@t> srhs.org (DeMatteo, Gabriela) Date: Fri Jun 6 09:23:41 2008 Subject: [Histonet] Dako Autostainer Message-ID: Would like to know if anyone is having problems with the Dako Autostainer Plus? Our instrument is giving the message that the certain antibody/reagent(s) are not found, but the tube(s) are in the rack in the correct spot. From bakevictoria <@t> gmail.com Fri Jun 6 09:49:53 2008 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Fri Jun 6 09:49:58 2008 Subject: [Histonet] Carlo Erba's Fixall Message-ID: <4f016b690806060749g68154768h438aca46521f117b@mail.gmail.com> Hi I am in the process of trying to find out the components of "Fixall" by Carlo Erba. Does anyone have a reference or possibly even used this reagent? Any information would be very, very welcomed! Thanks in advance. Vikki Baker From tanisha.mcknight <@t> covance.com Fri Jun 6 10:07:56 2008 From: tanisha.mcknight <@t> covance.com (McKnight, Tanisha) Date: Fri Jun 6 10:08:20 2008 Subject: [Histonet] NSH Histology Task Analysis and Standards of Performance Competen cy Message-ID: <816E3C72F855F14985FC31D7C963AE6F0762E989@indexch03.ent.covance.com> Hello Histo-netters: Would anyone happen to have an electronic copy of NSH Histology Task Analysis and The Standards of Performance/Competency that you could send to me? I am in desperate need of these items. I've contacted NSH for these twice, but I have gotten no response. I'd greatly appreciate anything you could send. Tanisha N. McKnight, HT (ASCP) Covance CLS Indianapolis Specimen Management, Anatomic Pathology ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From JMahoney <@t> alegent.org Fri Jun 6 10:22:44 2008 From: JMahoney <@t> alegent.org (Mahoney,Janice A) Date: Fri Jun 6 10:22:57 2008 Subject: [Histonet] NSH Histology Task Analysis and Standards of Performance Competen cy In-Reply-To: <816E3C72F855F14985FC31D7C963AE6F0762E989@indexch03.ent.covance.com> References: <816E3C72F855F14985FC31D7C963AE6F0762E989@indexch03.ent.covance.com> Message-ID: <346E5878979BA54FB4B0BFD6AD93B9B9B01F623379@EXCHMBC1.ad.ah.local> I'd love to have an electronic copy as well Jan Mahoney Alegent Health Omaha,NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McKnight, Tanisha Sent: Friday, June 06, 2008 10:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NSH Histology Task Analysis and Standards of Performance Competen cy Hello Histo-netters: Would anyone happen to have an electronic copy of NSH Histology Task Analysis and The Standards of Performance/Competency that you could send to me? I am in desperate need of these items. I've contacted NSH for these twice, but I have gotten no response. I'd greatly appreciate anything you could send. Tanisha N. McKnight, HT (ASCP) Covance CLS Indianapolis Specimen Management, Anatomic Pathology ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From trathborne <@t> somerset-healthcare.com Fri Jun 6 10:27:13 2008 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Fri Jun 6 10:30:25 2008 Subject: [Histonet] NSH Histology Task Analysis and Standards ofPerformance Competen cy In-Reply-To: <346E5878979BA54FB4B0BFD6AD93B9B9B01F623379@EXCHMBC1.ad.ah.local> Message-ID: As would I. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Mahoney,Janice A Sent: Friday, June 06, 2008 11:23 AM To: 'McKnight, Tanisha'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH Histology Task Analysis and Standards ofPerformance Competen cy I'd love to have an electronic copy as well Jan Mahoney Alegent Health Omaha,NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McKnight, Tanisha Sent: Friday, June 06, 2008 10:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NSH Histology Task Analysis and Standards of Performance Competen cy Hello Histo-netters: Would anyone happen to have an electronic copy of NSH Histology Task Analysis and The Standards of Performance/Competency that you could send to me? I am in desperate need of these items. I've contacted NSH for these twice, but I have gotten no response. I'd greatly appreciate anything you could send. Tanisha N. McKnight, HT (ASCP) Covance CLS Indianapolis Specimen Management, Anatomic Pathology ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. From godsgalnow <@t> aol.com Fri Jun 6 10:36:51 2008 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Fri Jun 6 10:37:05 2008 Subject: [Histonet] NSH Histology Task Analysis and Standards ofPerformance Competen cy In-Reply-To: Message-ID: <8CA9602462E04AF-F68-30D@webmail-db03.sysops.aol.com> I just got one...the only thing you have to do is email NSH and request one and they will email it to you Roxanne -----Original Message----- From: Rathborne, Toni To: Mahoney,Janice A ; McKnight, Tanisha ; histonet@lists.utsouthwestern.edu Sent: Fri, 6 Jun 2008 11:27 am Subject: RE: [Histonet] NSH Histology Task Analysis and Standards ofPerformance Competen cy As would I. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Mahoney,Janice A Sent: Friday, June 06, 2008 11:23 AM To: 'McKnight, Tanisha'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH Histology Task Analysis and Standards ofPerformance Competen cy I'd love to have an electronic copy as well Jan Mahoney Alegent Health Omaha,NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McKnight, Tanisha Sent: Friday, June 06, 2008 10:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NSH Histology Task Analysis and Standards of Performance Competen cy Hello Histo-netters: Would anyone happen to have an electronic copy of NSH Histology Task Analysis and The Standards of Performance/Competency that you could send to me? I am in desperate need of these items. I've contacted NSH for these twice, but I have gotten no response. I'd greatly appreciate anything you could send. Tanisha N. McKnight, HT (ASCP) Covance CLS Indianapolis Specimen Management, Anatomic Pathology ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDeMatteo <@t> srhs.org Fri Jun 6 10:44:08 2008 From: GDeMatteo <@t> srhs.org (DeMatteo, Gabriela) Date: Fri Jun 6 10:44:20 2008 Subject: [Histonet] Dako Autostainer Message-ID: About the same problem, beside the sensor malfunction, could the software be the problem? Our "immuno specialist" said that the service tech found nothing wrong with the Dako instrument. From Norm.Burnham <@t> propath.com Fri Jun 6 10:48:33 2008 From: Norm.Burnham <@t> propath.com (Norm Burnham) Date: Fri Jun 6 10:48:41 2008 Subject: [Histonet] NSH Histology Task Analysis and StandardsofPerformance Competen cy In-Reply-To: References: <346E5878979BA54FB4B0BFD6AD93B9B9B01F623379@EXCHMBC1.ad.ah.local> Message-ID: <332B4C097301BD42BEC7AA93D36CE5F1445510@mail.propathlab.com> Me too! Norm Burnham -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, June 06, 2008 10:27 AM To: Mahoney,Janice A; McKnight, Tanisha; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH Histology Task Analysis and StandardsofPerformance Competen cy As would I. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Mahoney,Janice A Sent: Friday, June 06, 2008 11:23 AM To: 'McKnight, Tanisha'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH Histology Task Analysis and Standards ofPerformance Competen cy I'd love to have an electronic copy as well Jan Mahoney Alegent Health Omaha,NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McKnight, Tanisha Sent: Friday, June 06, 2008 10:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NSH Histology Task Analysis and Standards of Performance Competen cy Hello Histo-netters: Would anyone happen to have an electronic copy of NSH Histology Task Analysis and The Standards of Performance/Competency that you could send to me? I am in desperate need of these items. I've contacted NSH for these twice, but I have gotten no response. I'd greatly appreciate anything you could send. Tanisha N. McKnight, HT (ASCP) Covance CLS Indianapolis Specimen Management, Anatomic Pathology ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. ______________________________________________________________________________ This e-mail may contain confidential or privileged information. If you think you have received this e-mail in error, please advise the sender by reply e-mail and then delete this e-mail immediately. From Aubrey <@t> nsh.org Fri Jun 6 10:49:14 2008 From: Aubrey <@t> nsh.org (Aubrey Wanner) Date: Fri Jun 6 10:49:35 2008 Subject: [Histonet] RE: NSH Histology Task Analysis and Standards of Performance In-Reply-To: References: Message-ID: Electronic copies of the Task Analysis and Standards of Performance documents are available to NSH Members Only. If you are an NSH member please email your request to histo@nsh.org. Mrs. Aubrey M.J. Wanner Meeting Manager National Society for Histotechnology 10320 Little Patuxent Parkway | Suite 804 Columbia, MD 21044 Main | 443.535.4060 Direct | 443.535.4065 Fax | 443.535.4055 http://www.nsh.org/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, June 06, 2008 11:40 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 55, Issue 11 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Clarification of Legality of work (joelle weaver) 2. fed. regulations child labor (joelle weaver) 3. Decolorize PAS (Christine Bark) 4. Re: LIS (Emily Sours) 5. Re: question (Anthony Reilly) 6. electrical adapter for Mexico (suboro) 7. Re: LIS (Matt Bancroft) 8. RE: question (Tony Henwood) 9. Re: Legality of work (Markus F. Meyenhofer) 10. Re: Legality of work (Mark Tarango) 11. Re: Legality of work (Emily Sours) 12. Re: Need Help from Mexico (Rene J Buesa) 13. Re: Decolorize PAS (Rene J Buesa) 14. unsubscribe (Beverly Robinson) 15. Dako Autostainer (DeMatteo, Gabriela) 16. Carlo Erba's Fixall (Victoria Baker) 17. NSH Histology Task Analysis and Standards of Performance Competen cy (McKnight, Tanisha) 18. RE: NSH Histology Task Analysis and Standards of Performance Competen cy (Mahoney,Janice A) 19. RE: NSH Histology Task Analysis and Standards ofPerformance Competen cy (Rathborne, Toni) 20. Re: NSH Histology Task Analysis and Standards ofPerformance Competen cy (godsgalnow@aol.com) ---------------------------------------------------------------------- Message: 1 Date: Thu, 5 Jun 2008 21:12:17 +0000 From: joelle weaver Subject: RE: [Histonet] Clarification of Legality of work To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" I just wanted to say that the suggestion below seems to be a good one. I think that it is good practice to formally bring your concerns and complaints directly to the person who may attend to them. That being said, tact and diplomacy can help here. If you get a bad response, you have convered yourself and given them the opportunity to resond, correct and explain. But, I do think that the suggestion to push the formal act of officially hiring a minor does place the responsibility directly on the party employing her with regards to her rights to safety, appropriate PPE, and training -that other "offical" employees enjoy. This may resolve the situation. But it sounds to me that there are other interpersonal issues as well? However, clearing one issue can only serve to lessen the stress and tension. Hope this helps? Joelle Weaver BA, HTL (ASCP)> Date: Thu, 5 Jun 2008 15:51:45 -0500> From: JMcCormick@schosp.org> To: freckles9660@yahoo.com; histonet@lists.utsouthwestern.edu> Subject: RE: [Histonet] Clarification of Legality of work> CC: > > Second response: from "an interested pathologist" > When I was a young man, I too was called "freckles !> > Why not take all of the information that you have gathered to your pathologist/boss with the concerns expressed. > Ask him to clear the road by HIS formal employment of his daughter and when properly employed (at his risk) bring her into the lab as an apprentice. In this role she should have the responsibility ( on work time) to WRITE weekly reports on her work. I have a hunch that this routine might be too rigorous for her to last. On the other hand, if she flourishes with success in an agenda of progression that has been assigned to her apprentice role....you may have contributed to motivating another quality person to our profession. Will she resume and complete her formal schooling in the fall???..........good idea ! > > Good luck,> J.B.McCormick, M.D,> > > > > -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Karla Arrington> Sent: Thursday, June 05, 2008 3:07 PM> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] Clarification of Legality of work> > Fellow Histo's:> > Thanks for the many responses. But I feel that I have to clarify the situation. In this state, HT's are very hard to come by. So with that in mind, the Pathologist suggested his 15 year old daughter to help me, working summers and holidays. The plan is for me to train her as a Histology student taking their clinical. Also, the Pathologist wants her to take the on-line HT program to get certified. Do you not have to have a high school diploma first? She is only 15!! She is also to be paid by the companies payroll. She has not been exposed to any of the basics. So therefore, I am "teaching" a minor every aspect of Histology. When I need time off, she will be responsible for the work that is performed. This includes embedding, microtomy, staining, special stains, IHC's, maintenance; etc. She would be a 15 year old doing the work of a certified HT. To this I feel very uncomfortable. In response, I called our state Child Labor law> department and they said that it is ILLEGAL for a minor, under the age of 17 to be working in the medical laboratory, especially with blood borne pathogens. When she is here, I am solely responsible for her as her parent is usually unavailable, so therefore no direct supervision by the Pathologist. If "caught", I don't want to loose my HT license because of this. If I disagree> to not "teach" his daughter, I know there will be consequences for me. And that leads to the second dilemma.> Any suggestion?> > Concerned, > freckles9660@yahoo.com> > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > *** Confidentiality Statement ***> This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction.> > > Thank you for your cooperation.> > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Search that pays you back! Introducing Live Search cashback. http://search.live.com/cashback/?&pkw=form=MIJAAF/publ=HMTGL/crea=srchpaysyouback ------------------------------ Message: 2 Date: Thu, 5 Jun 2008 21:19:00 +0000 From: joelle weaver Subject: [Histonet] fed. regulations child labor To: Message-ID: Content-Type: text/plain; charset="Windows-1252" Hi all http://www.dol.gov/dol/topic/youthlabor/agerequirements.htm#lawregs Fair Labor Standards Act (FLSA)-The rules vary depending upon the particular age of the minor and the particular job involved. As a general rule, the FLSA sets 14 years of age as the minimum age for employment, and limits the number of hours worked by minors under the age of 16. Also, the FLSA generally prohibits the employment of a minor in work declared hazardous by the Secretary of Labor (for example, work involving excavation, driving, and the operation of many types of power-driven equipment). The FLSA contains a number of requirements that apply only to particular types of jobs (for example, agricultural work or the operation of motor vehicles) and many exceptions to the general rules (for example, work by a minor for his or her parents). Each state also has its own laws relating to employment, including the employment of minors. If state law and the FLSA overlap, the law which is more protective of the minor will apply. The FLSA "covers" or applies to all employees of certain "enterprises." All employees of an enterprise, as defined by the FLSA, are covered regardless of the duties they perform. If a worker is not an employee of one of these enterprises, he or she may still be covered if the employee's own duties meet certain interstate commerce requirements "enterprise" inlcudes: A hospital, or an institution primarily engaged in the care of the sick, the aged, or the mentally ill or mentally retarded who live on the premises (it does not matter if the hospital or institution is public or private or is operated for profit or not-for-profit) Regulations29 CFR ?570.2 Minimum Age Standards for Nonagricultural Employment 29 CFR ??570.31-34 Thanks, Joelle Weaver BA, HTL(ASCP) _________________________________________________________________ It's easy to add contacts from Facebook and other social sites through Windows Live(tm) Messenger. Learn how. https://www.invite2messenger.net/im/?source=TXT_EML_WLH_LearnHow ------------------------------ Message: 3 Date: Thu, 5 Jun 2008 14:30:53 -0700 From: "Christine Bark" Subject: [Histonet] Decolorize PAS To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Hello all, My pathologist asked me to de-stain a PAS-stained section of liver and re-stain it with a trichrome. I've never decolorized anything other than an H&E. Would I still use acid alcohol and if so for how long? I've read that potassium metabisulfite will take out excess Schiff's but I've never tried that and I don't have any. Any suggestions/tips? Thank you, Christine Bark HT(ASCP) ______________________________________________________________________________ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 4 Date: Thu, 5 Jun 2008 19:39:57 -0400 From: "Emily Sours" Subject: Re: [Histonet] LIS To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 What does one use pathology software for? Just organizing files? Emily -- When you're riding in a time machine way far into the future, don't stick your elbow out the window, or it'll turn into a fossil. ------------------------------ Message: 5 Date: Fri, 06 Jun 2008 09:56:01 +1000 From: "Anthony Reilly" Subject: Re: [Histonet] question To: , "Stephen Peters M.D." Message-ID: <484909AF.471C.0039.0@health.qld.gov.au> Content-Type: text/plain; charset="us-ascii" Kathy You have appeared to try every bluing agent possible, however in my experience the most common cause of pale nuclei in frozen sections is inadequate fixation. What are you using? The best I have used is 5% Conc Formalin in 95% Ethanol for at least 30 sec and longer if time permits. All the best. Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_reilly@health.qld.gov.au >>> "Stephen Peters M.D." 6/06/2008 1:34 am >>> Kathy, You may also want to consider the thickness of your sections. A great deal of our information is gatered at scanning magnification of 2x or 4x. If you are cutting at 3 or 4 microns or if your cryostat is offering a variety of thick and thin the slides will be pale at these powers compared to 5 or 6 micron sections. Presence of a lot of nuclear "holes"is a sign it is cut very thin. Also make sure your hematoxalin is changed regularly and is not growing "rock candy" in the bottom. Check slide after it leaves the bluing and get to know the shade of blue that represents a well stained slide. Remember when checking the slides, the amount of blue will depend on the densityof nuclear material as well as the thickness of the tissue. I find the actual shade of blue tells me that it has been stained in hemotoxalin long enough. Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** ------------------------------ Message: 6 Date: Thu, 5 Jun 2008 17:05:17 -0700 (PDT) From: suboro Subject: [Histonet] electrical adapter for Mexico To: histonet@lists.utsouthwestern.edu Message-ID: <81297.32717.qm@web34703.mail.mud.yahoo.com> Content-Type: text/plain; charset=us-ascii Shirley, You can find power adapters at travel stores, or even WalMart. The one I took to Honduras had the various countries or regions where it might be applicable listed right on the package. BTW, we were not allowed to carry on our scope. Security at the gate informed us only one carry-on into Honduras was allowed, so we had to make some quick and scary decisions; the poor scope had to go with baggage. Call your airlines to see if Mexico is similar, and at least your scope can be packed appropriately.     Susan Beam me up, Scotty ------------------------------ Message: 7 Date: Thu, 5 Jun 2008 17:27:15 -0700 (PDT) From: Matt Bancroft Subject: Re: [Histonet] LIS To: histonet@lists.utsouthwestern.edu, Araceli Davis Message-ID: <946183.93981.qm@web63414.mail.re1.yahoo.com> Content-Type: text/plain; charset=us-ascii AP Easy   You can look at it at APEASY.com   --- On Thu, 6/5/08, Araceli Davis <ardavis@wlgore.com> wrote: From: Araceli Davis <ardavis@wlgore.com> Subject: [Histonet] LIS To: histonet@lists.utsouthwestern.edu Date: Thursday, June 5, 2008, 12:50 PM Hi, we are looking for pathology software for a small research lab. Does any one have any recommendations? Araceli _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Fri, 6 Jun 2008 10:42:50 +1000 From: "Tony Henwood" Subject: RE: [Histonet] question To: "Anthony Reilly" , , "Stephen Peters M.D." Message-ID: Content-Type: text/plain; charset="us-ascii" I concur (now that's an old word) Or, how about: I am unanimous in this Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anthony Reilly Sent: Friday, 6 June 2008 9:56 AM To: Histonet@lists.utsouthwestern.edu; Stephen Peters M.D. Subject: Re: [Histonet] question Kathy You have appeared to try every bluing agent possible, however in my experience the most common cause of pale nuclei in frozen sections is inadequate fixation. What are you using? The best I have used is 5% Conc Formalin in 95% Ethanol for at least 30 sec and longer if time permits. All the best. Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Level 1, Building 15 Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:07 32402930 tony_reilly@health.qld.gov.au >>> "Stephen Peters M.D." 6/06/2008 1:34 am >>> Kathy, You may also want to consider the thickness of your sections. A great deal of our information is gatered at scanning magnification of 2x or 4x. If you are cutting at 3 or 4 microns or if your cryostat is offering a variety of thick and thin the slides will be pale at these powers compared to 5 or 6 micron sections. Presence of a lot of nuclear "holes"is a sign it is cut very thin. Also make sure your hematoxalin is changed regularly and is not growing "rock candy" in the bottom. Check slide after it leaves the bluing and get to know the shade of blue that represents a well stained slide. Remember when checking the slides, the amount of blue will depend on the densityof nuclear material as well as the thickness of the tissue. I find the actual shade of blue tells me that it has been stained in hemotoxalin long enough. Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************ ******** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ************************************************************************ ********** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 9 Date: Thu, 5 Jun 2008 22:04:50 -0400 From: "Markus F. Meyenhofer" Subject: Re: [Histonet] Legality of work To: "McCormick, James" , "Rene J Buesa" , "Karla Arrington" , Message-ID: <09ac01c8c779$b61922d0$a3893cd1@DJ4VDH31> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Thank you! Regards, Markus F. Meyenhofer Microscopy Labs Red Bank, NJ ----- Original Message ----- From: "McCormick, James" To: "Rene J Buesa" ; "Karla Arrington" ; Sent: Thursday, June 05, 2008 9:29 AM Subject: RE: [Histonet] Legality of work >From an interested Pathologist: I think you are building a bomb to attack an mouse! When our son and daughter were 14 and 15 years old I was proud to have them VOLUNTEER (non-paid) service to the our laboratory. First to meet the fine people who worked in the department. Second to learn about "dad's work", and third to develop a sense of appreciation for our work in serving others. The children benefited by the social relationship and grew in responsibility by graduating from tasks of simple "go for's", observers, awarded responsibility. They were not permitted to prepare patient materials but they were, over a period of summer volunteer effort, taught to make a collection of their own slides and even to do a frozen section. The experience is a treasured memory and valued experience for the Pathologist and his family and for the bonding of employees who for that period were extended members of the family. Our son is now completing his residency in orthopaedic Surgery and our daughter is a junior officer in a New York company where she has employees reporting to her service. I continue to communicate with the lab employees and they always ask .....tell us how your son and daughter are doing and send them our love. The well operated pathology lab is no more dangerous than a high school biology and chemistry laboratory. I believe you would do yourself,the lab.,the pathologist and the 15 year old a great service by reexamining the situation in a more generous and understanding way. If you are at odds with the pathologist.... take time to visit with him and structure a helping and learning experience for the young lady who just happens to be your bosses daughter. Ask the young lady to observe and take notes for a week. Chart the path of a patient specimen from the beginning through the final diagnosis. Write (required) a report of the reason the specimen was sent to the lab. The complete process/path of the specimen through the lab,and the final result/diagnosis for the patient's benefit. If the 15 year old does this there will be a change in the climate and "dad" will be amazed at what you contribute to the process of health care and to the maturing of his daughter. Just a few thoughts from a Pathologist and dad with success written in the log of his children and their bonding to the profession. Sincerely, and in the spirit of helping. Please give a copy of this note to your pathologist. Kindest regards, J.B.McCormick, M.D. jmccormi@schosp.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, June 05, 2008 7:51 AM To: Karla Arrington; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Legality of work Here is how I see your problem: 1- you have already contacted the Child and Labor Dept. about this issue, so it is very likely (if they are doing their job) that the will "pay a visit" to the lab and talk with the pathologist. 2- if that is what is going to happen you are going to be in trouble. 3- as the "youngster's" trainer you are accomplice to the violation, because you know her age and keep training her. 4- if this pathologist is also calling names to others, he is an absolute jerk that thinks is owner, not only of the place, but of the world. 5- I don't think this is the right place to work so sooner or later you should try to find a new place to work. 6- if that is going to be how this whole issue is going to end, I think that you should do what is correct and tell him that you are not willing to participate in violating the laboral laws and stop training his daughter. You will sleep better and perhaps this fellow will realize that he is doing something wrong and that you are not willing to accept the situation. At least that is what I would do. Consult with some close friend or family member and present a formal complaint with Child Labor BEFORE doing #6. If he fires you because of this then you will have PROOF that you complained against him because of a law violation and will be able to claim unemployment, even if he says that you were fired for "work substandard performance", you will have proof that this is not the case, but revenge against you. Good luck! Ren? J. Karla Arrington wrote: Histo's: I have roughly 2 dilemmas. The first is of a legal matter. The Pathologist for a week has had his daughter (15 years old), helping me out in the histology lab. He wants her to get training from me, then do the on-line HT program. She has handled chemicals and reagents. I am very uncomfortable with this. I have called the Child Labor Laws department for our state and it is illegal for a 15 year to be in a laboratory. Needless to say working with blood borne pathogens. My most concern is the following. As her "teacher", can I or other co-workers can be held accountable if this is illegal and is found out? This pathologist mind you is the owner of the business. I am afraid if I say something, I will get fired. Where do I go from here or if there is someone who has a similar circumstance happen to them. The other scenario is that this same Pathologist has called a tech a slandering name, twice. There is no "upper management" to go to since he is the owner of the business. I was wondering if this is considered harassment and can this be used to nullify a contract signed by both parties for employment. Concerned, freckles9660@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Thu, 5 Jun 2008 22:14:12 -0700 From: "Mark Tarango" Subject: Re: [Histonet] Legality of work To: "Karla Arrington" Cc: histonet@lists.utsouthwestern.edu Message-ID: <5b6eb13e0806052214s24f0fd22le367585744814b07@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi Karla, If it turns out that there is nothing illegal about training her, would you still have a problem doing it? You mentioned that the pathologist has called someone a name. I'm guessing that he might not be well liked. If that's the case, would this be something that his daughter would be likely to notice? One final question, are you the only HT in the lab? On Wed, Jun 4, 2008 at 4:37 PM, Karla Arrington wrote: > Histo's: > > I have roughly 2 dilemmas. The first is of a legal matter. The > Pathologist for a week has had his daughter (15 years old), helping > me out in the histology lab. He wants her to get training from me, then do > the on-line HT program. She has handled chemicals > and reagents. I am very uncomfortable with this. I have called the Child > Labor Laws department for our state and it is illegal > for a 15 year to be in a laboratory. Needless to say working with blood > borne pathogens. My most concern is the following. As her "teacher", > can I or other co-workers can be held accountable if this is illegal and is > found out? This pathologist mind you is the owner of the business. > I am afraid if I say something, I will get fired. Where do I go from here > or if there is someone who has a similar circumstance happen to them. > The other scenario is that this same Pathologist has called a tech a > slandering name, twice. There is no "upper management" to go to > since he is the owner of the business. I was wondering if this is > considered harassment and can this be used to nullify a contract signed > by both parties for employment. > > Concerned, > freckles9660@yahoo.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 11 Date: Fri, 6 Jun 2008 03:15:23 -0400 From: "Emily Sours" Subject: Re: [Histonet] Legality of work To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 With no experience outside of the academic lab, let me tell you: break every taboo you can. Especially if it deals with a daughter and father--put her in control (ie she knows what she's doing, or at least you taught her that) and keep the father in it as little as possible. This is what we need in our labs! It takes a small change to create a fantastic lab--I hope you create that, regardless of the company's position in the stock market or expected financial ground. I speak from my idealistic grant world (in which, believe me, they don't afford me anything over 2.5% living wage of 29000, the latest), yet I love my job and wouldn't give it up for the world. (Background: 31 with a BS in microbio, been a technician for 9 years and loving it) Consider that when you get a kid--would they love it as much as a geek like me? I <3 my science job, Emily -- When you're riding in a time machine way far into the future, don't stick your elbow out the window, or it'll turn into a fossil. ------------------------------ Message: 12 Date: Fri, 6 Jun 2008 05:56:19 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Need Help from Mexico To: Shirley Powell , histonet@lists.utsouthwestern.edu Message-ID: <759763.62935.qm@web65713.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Electrical power in Mexico is the same voltage as in the US, even the electrical outlets are identical. Just returned from Mexico. Ren? J. Shirley Powell wrote: Hi histonetters, One of my pathologists here at Mercer is presently preparing to go on a medical mission trip to Mexico with a group of our medical students. She has a somewhat old but beloved AO microscope (model is an 1130A) to take with her. The bulb is an Osram halogen xenophot with the letters/numbers on it - HLX 64250 ESB 6V 20W. There is no switch to select voltages. Do you have a recommendation for any required adapter, she was told they will have power (possibly from a generator) where the clinic will be located but does not know what voltage to expect. Any help will be appreciated. I know nothing of electricity requirements in other countries and we are trying to get all that they will need while there. Thanks! Shirley Powell Mercer University School of Medicine Technical Director Histology Curricular Support Lab 1550 College Street Macon, GA 31207 478-301-2374 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Fri, 6 Jun 2008 06:03:46 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Decolorize PAS To: Christine Bark , histonet@lists.utsouthwestern.edu Message-ID: <811514.42631.qm@web65710.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 PAS is a very strong reaction extremely difficult to destain. Acid treatments that could fade it will affect the reactivity of the tissue for further staining procedures. Ren? J. Christine Bark wrote: Hello all, My pathologist asked me to de-stain a PAS-stained section of liver and re-stain it with a trichrome. I've never decolorized anything other than an H&E. Would I still use acid alcohol and if so for how long? I've read that potassium metabisulfite will take out excess Schiff's but I've never tried that and I don't have any. Any suggestions/tips? Thank you, Christine Bark HT(ASCP) ______________________________________________________________________________ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Fri, 6 Jun 2008 10:10:08 -0400 From: Beverly Robinson Subject: [Histonet] unsubscribe To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Please remove me / unsubscribe me from the histonet list ------------------------------ Message: 15 Date: Fri, 6 Jun 2008 10:22:18 -0400 From: "DeMatteo, Gabriela" Subject: [Histonet] Dako Autostainer To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Would like to know if anyone is having problems with the Dako Autostainer Plus? Our instrument is giving the message that the certain antibody/reagent(s) are not found, but the tube(s) are in the rack in the correct spot. ------------------------------ Message: 16 Date: Fri, 6 Jun 2008 10:49:53 -0400 From: "Victoria Baker" Subject: [Histonet] Carlo Erba's Fixall To: histonet Message-ID: <4f016b690806060749g68154768h438aca46521f117b@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi I am in the process of trying to find out the components of "Fixall" by Carlo Erba. Does anyone have a reference or possibly even used this reagent? Any information would be very, very welcomed! Thanks in advance. Vikki Baker ------------------------------ Message: 17 Date: Fri, 6 Jun 2008 11:07:56 -0400 From: "McKnight, Tanisha" Subject: [Histonet] NSH Histology Task Analysis and Standards of Performance Competen cy To: histonet@lists.utsouthwestern.edu Message-ID: <816E3C72F855F14985FC31D7C963AE6F0762E989@indexch03.ent.covance.com> Content-Type: text/plain; charset="us-ascii" Hello Histo-netters: Would anyone happen to have an electronic copy of NSH Histology Task Analysis and The Standards of Performance/Competency that you could send to me? I am in desperate need of these items. I've contacted NSH for these twice, but I have gotten no response. I'd greatly appreciate anything you could send. Tanisha N. McKnight, HT (ASCP) Covance CLS Indianapolis Specimen Management, Anatomic Pathology ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. ------------------------------ Message: 18 Date: Fri, 6 Jun 2008 10:22:44 -0500 From: "Mahoney,Janice A" Subject: RE: [Histonet] NSH Histology Task Analysis and Standards of Performance Competen cy To: "'McKnight, Tanisha'" , "histonet@lists.utsouthwestern.edu" Message-ID: <346E5878979BA54FB4B0BFD6AD93B9B9B01F623379@EXCHMBC1.ad.ah.local> Content-Type: text/plain; charset="us-ascii" I'd love to have an electronic copy as well Jan Mahoney Alegent Health Omaha,NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McKnight, Tanisha Sent: Friday, June 06, 2008 10:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NSH Histology Task Analysis and Standards of Performance Competen cy Hello Histo-netters: Would anyone happen to have an electronic copy of NSH Histology Task Analysis and The Standards of Performance/Competency that you could send to me? I am in desperate need of these items. I've contacted NSH for these twice, but I have gotten no response. I'd greatly appreciate anything you could send. Tanisha N. McKnight, HT (ASCP) Covance CLS Indianapolis Specimen Management, Anatomic Pathology ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ------------------------------ Message: 19 Date: Fri, 6 Jun 2008 11:27:13 -0400 From: "Rathborne, Toni" Subject: RE: [Histonet] NSH Histology Task Analysis and Standards ofPerformance Competen cy To: "Mahoney,Janice A" , "McKnight, Tanisha" , Message-ID: Content-Type: text/plain; charset="utf-8" As would I. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Mahoney,Janice A Sent: Friday, June 06, 2008 11:23 AM To: 'McKnight, Tanisha'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH Histology Task Analysis and Standards ofPerformance Competen cy I'd love to have an electronic copy as well Jan Mahoney Alegent Health Omaha,NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McKnight, Tanisha Sent: Friday, June 06, 2008 10:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NSH Histology Task Analysis and Standards of Performance Competen cy Hello Histo-netters: Would anyone happen to have an electronic copy of NSH Histology Task Analysis and The Standards of Performance/Competency that you could send to me? I am in desperate need of these items. I've contacted NSH for these twice, but I have gotten no response. I'd greatly appreciate anything you could send. Tanisha N. McKnight, HT (ASCP) Covance CLS Indianapolis Specimen Management, Anatomic Pathology ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. ------------------------------ Message: 20 Date: Fri, 06 Jun 2008 11:36:51 -0400 From: godsgalnow@aol.com Subject: Re: [Histonet] NSH Histology Task Analysis and Standards ofPerformance Competen cy To: trathborne@somerset-healthcare.com, JMahoney@alegent.org, tanisha.mcknight@covance.com, histonet@lists.utsouthwestern.edu Message-ID: <8CA9602462E04AF-F68-30D@webmail-db03.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" I just got one...the only thing you have to do is email NSH and request one and they will email it to you Roxanne -----Original Message----- From: Rathborne, Toni To: Mahoney,Janice A ; McKnight, Tanisha ; histonet@lists.utsouthwestern.edu Sent: Fri, 6 Jun 2008 11:27 am Subject: RE: [Histonet] NSH Histology Task Analysis and Standards ofPerformance Competen cy As would I. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Mahoney,Janice A Sent: Friday, June 06, 2008 11:23 AM To: 'McKnight, Tanisha'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH Histology Task Analysis and Standards ofPerformance Competen cy I'd love to have an electronic copy as well Jan Mahoney Alegent Health Omaha,NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McKnight, Tanisha Sent: Friday, June 06, 2008 10:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NSH Histology Task Analysis and Standards of Performance Competen cy Hello Histo-netters: Would anyone happen to have an electronic copy of NSH Histology Task Analysis and The Standards of Performance/Competency that you could send to me? I am in desperate need of these items. I've contacted NSH for these twice, but I have gotten no response. I'd greatly appreciate anything you could send. Tanisha N. McKnight, HT (ASCP) Covance CLS Indianapolis Specimen Management, Anatomic Pathology ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 55, Issue 11 **************************************** From sbreeden <@t> nmda.nmsu.edu Fri Jun 6 11:28:03 2008 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Jun 6 11:28:09 2008 Subject: [Histonet] Sally's Friday Fume Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E636F@nmdamailsvr.nmda.ad.nmsu.edu> Okay, I finally have something to Fume about. I am beginning to train a person to become a certified histologist. She is a certified veterinary tech with an Associates Degree and the appropriate number of chemistry/biology hours. I inquired of ASCP about her eligibility (after we finish the training period) to take the HT exam. According to ASCP, as a vet diagnostic lab, we do not meet the ASCP requirements for eligibility because we are not accredited by CAP, JCAHO/AABB and do not have a board-certified (read "human") pathologist/medical scientist (hmmm? Could one of our veterinary pathologists fulfill the description of "medical scientist"??). Surely there are other persons working in a veterinary pathology lab that would be interested in certification, one would think. What are they to do? This frosts my petunias and I'd like to know if anyone has any experience or input on this. But - hey! - some of my fluids/solutions in gallon/liter containers seem to have been improved so they don't drip, so maybe someone out there IS listening (that was my first Friday Hour of Fuming subject). Hasta lumbago. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From victor <@t> pathology.washington.edu Fri Jun 6 11:33:51 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Fri Jun 6 11:34:00 2008 Subject: [Histonet] Sally's Friday Fume In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E636F@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B017E636F@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <484966EF.3000609@pathology.washington.edu> Have the rules changed? I supervised 2 Vet Histology labs from the late 80's to the end of the 90's and had several techs get certified. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Breeden, Sara wrote: > Okay, I finally have something to Fume about. I am beginning to train a > person to become a certified histologist. She is a certified veterinary > tech with an Associates Degree and the appropriate number of > chemistry/biology hours. I inquired of ASCP about her eligibility (after > we finish the training period) to take the HT exam. According to ASCP, > as a vet diagnostic lab, we do not meet the ASCP requirements for > eligibility because we are not accredited by CAP, JCAHO/AABB and do not > have a board-certified (read "human") pathologist/medical scientist > (hmmm? Could one of our veterinary pathologists fulfill the description > of "medical scientist"??). Surely there are other persons working in a > veterinary pathology lab that would be interested in certification, one > would think. What are they to do? This frosts my petunias and I'd like > to know if anyone has any experience or input on this. But - hey! - some > of my fluids/solutions in gallon/liter containers seem to have been > improved so they don't drip, so maybe someone out there IS listening > (that was my first Friday Hour of Fuming subject). Hasta lumbago. > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 4700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From liz <@t> premierlab.com Fri Jun 6 11:39:45 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Jun 6 11:39:50 2008 Subject: [Histonet] Sally's Friday Fume In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E636F@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B017E636F@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: Sally We are a contract lab and we do not have a pathologist on staff. When my tech applied to take the HTL I had to write a letter to the BOR for an exception which they granted for her to sit for the HTL registry. Since they do require a patholgist to sign for experience. Also later when we both wanted to take the IHC qualification we again wrote a letter to the BOR for an exception. I'm thinking the person at the BOR is not correct unless the rules have changed but they better not have. I have 4 techs here that are currently studying to take the HT and HTL and two other techs from local biotech companies that come here every two weeks for lectures, if this is correct then they would not be eligible to sit for the exam either. I find this hard to believe. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, June 06, 2008 10:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sally's Friday Fume Okay, I finally have something to Fume about. I am beginning to train a person to become a certified histologist. She is a certified veterinary tech with an Associates Degree and the appropriate number of chemistry/biology hours. I inquired of ASCP about her eligibility (after we finish the training period) to take the HT exam. According to ASCP, as a vet diagnostic lab, we do not meet the ASCP requirements for eligibility because we are not accredited by CAP, JCAHO/AABB and do not have a board-certified (read "human") pathologist/medical scientist (hmmm? Could one of our veterinary pathologists fulfill the description of "medical scientist"??). Surely there are other persons working in a veterinary pathology lab that would be interested in certification, one would think. What are they to do? This frosts my petunias and I'd like to know if anyone has any experience or input on this. But - hey! - some of my fluids/solutions in gallon/liter containers seem to have been improved so they don't drip, so maybe someone out there IS listening (that was my first Friday Hour of Fuming subject). Hasta lumbago. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Fri Jun 6 11:46:54 2008 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Jun 6 11:46:58 2008 Subject: [Histonet] Sally's Friday Fume References: <4D14F0FC9316DD41972D5F03C070908B017E636F@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <00ca01c8c7f4$ed25e350$a3065486@auxs.umn.edu> Most (if not all) veterinary diagnostic laboratories are certified through the AAVLD (American Association of Veterinary Laboratory Diagnosticians) and they are staffed by board-certified pathologists. One would surely think that these specifications would meet ASCP's requirements as an institution for anyone wishing to sit for the exam. Jan Shivers Senior Scientist Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu ----- Original Message ----- From: "Breeden, Sara" To: Sent: Friday, June 06, 2008 11:28 AM Subject: [Histonet] Sally's Friday Fume Okay, I finally have something to Fume about. I am beginning to train a person to become a certified histologist. She is a certified veterinary tech with an Associates Degree and the appropriate number of chemistry/biology hours. I inquired of ASCP about her eligibility (after we finish the training period) to take the HT exam. According to ASCP, as a vet diagnostic lab, we do not meet the ASCP requirements for eligibility because we are not accredited by CAP, JCAHO/AABB and do not have a board-certified (read "human") pathologist/medical scientist (hmmm? Could one of our veterinary pathologists fulfill the description of "medical scientist"??). Surely there are other persons working in a veterinary pathology lab that would be interested in certification, one would think. What are they to do? This frosts my petunias and I'd like to know if anyone has any experience or input on this. But - hey! - some of my fluids/solutions in gallon/liter containers seem to have been improved so they don't drip, so maybe someone out there IS listening (that was my first Friday Hour of Fuming subject). Hasta lumbago. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sjchtascp <@t> yahoo.com Fri Jun 6 12:31:58 2008 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Fri Jun 6 12:32:01 2008 Subject: [Histonet] Looking for Work Message-ID: <193638.55208.qm@web38201.mail.mud.yahoo.com> I reside in South Central WI. Looking for work in this area, No. WI, No.Mich. VT, or NH. Thanks everyone and have a "grand" weekend. Steve From jcline <@t> wchsys.org Fri Jun 6 12:39:12 2008 From: jcline <@t> wchsys.org (Joyce Cline) Date: Fri Jun 6 12:39:27 2008 Subject: [Histonet] RE: Sally's Friday Fume Message-ID: <74D382FF830A469BA98DB51E36C9FBF5@wchsys.org> I received my HT while I worked in research, under a veterinarian pathologist. ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From sbreeden <@t> nmda.nmsu.edu Fri Jun 6 13:03:39 2008 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Jun 6 13:03:45 2008 Subject: [Histonet] Veterinary Histologist Certification Eligibility Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E6370@nmdamailsvr.nmda.ad.nmsu.edu> That's a mouthful! Anyway - thank you to those who responded to me. I searched out a board-certified pathologist (non-veterinary) who works in our building (we share a State of NM building with OMI and the State Laboratory) and he was as astounded as I. I appreciate the feedback about writing a letter to BOR requesting a "waiver" for a veterinary situation and I will try that when the time comes. Our lab, in anticipation of our new building (2010) is operating under conditional AALVD certification but certainly not under CAP/JCHAO. I'm thinking of asking for a definition of "medical scientist" from the ASCP. The saga continues, but "where there's a will, there's a way". One would think anyone who is interested in becoming a certified histologist would receive the maximum amount of encouragement, from the employer AND the certifying agency. Perhaps it's time for a review of the requirements or at least of the definition of an employer's validity to provide appropriate training, hmmm??? Mr. Della Speranza, do you have a take on this? It is not my goal here to fluster, annoy, or pester. It's just that I'm beginning to see a certain amount of reluctance on the part of those institutions whose interest should be to encourage the growth of our profession. Now that my aim is to interest local mid-high and high school forensic science students in a detour to the Paraffin Side, I have a path littered with roadblocks. If our profession had the same PR firm that the anti-tobacco industry had, we'd be up to our earlobes in histotechs (did that come out right?). Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From Christen <@t> vet.k-state.edu Fri Jun 6 13:14:12 2008 From: Christen <@t> vet.k-state.edu (Shelly Christenson) Date: Fri Jun 6 13:14:36 2008 Subject: [Histonet] Sally's Friday Fume In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E636F@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B017E636F@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <48493823.EF61.003F.0@vet.k-state.edu> I work in a veterinary diagnostic lab and got my HT certification in 1993 without any problems and they accepted our veterinary pathologist signature. Two other techs got certified later and no problems there either. Shelly Christenson HT(ASCP) Kansas State University Veterinary Diagnostic Lab Manhattan, Kansas 66506 >>> "Breeden, Sara" 6/6/2008 11:28 AM >>> Okay, I finally have something to Fume about. I am beginning to train a person to become a certified histologist. She is a certified veterinary tech with an Associates Degree and the appropriate number of chemistry/biology hours. I inquired of ASCP about her eligibility (after we finish the training period) to take the HT exam. According to ASCP, as a vet diagnostic lab, we do not meet the ASCP requirements for eligibility because we are not accredited by CAP, JCAHO/AABB and do not have a board-certified (read "human") pathologist/medical scientist (hmmm? Could one of our veterinary pathologists fulfill the description of "medical scientist"??). Surely there are other persons working in a veterinary pathology lab that would be interested in certification, one would think. What are they to do? This frosts my petunias and I'd like to know if anyone has any experience or input on this. But - hey! - some of my fluids/solutions in gallon/liter containers seem to have been improved so they don't drip, so maybe someone out there IS listening (that was my first Friday Hour of Fuming subject). Hasta lumbago. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dawn.Gullifer <@t> osumc.edu Fri Jun 6 13:31:31 2008 From: Dawn.Gullifer <@t> osumc.edu (Gullifer, Dawn) Date: Fri Jun 6 13:31:41 2008 Subject: [Histonet] Job Opening Columbus, OH Message-ID: All Interested- We have a job opening for a Anatomic Pathology Technologist at OSU Histology Lab, LLC. We are located in Columbus Ohio and are affiliated with The Ohio State University Medical Center. The duties include accessioning, gross examination of biopsy specimens including dictation, preparing tissue for processing and all other normal laboratory duties (inventory, recording, supplies etc....). Experience Requirements are a 2 yr. associates degree in science related field or minimum 24 semester hours (36 quarter hours) of biology, chemistry, physics, and math. The Job Qualifications include training or experience as a tissue prosector desired; certification or experience as a histology technologist desired; and certification, training or experience as an anatomic pathology technician desired. For additional information and for qualifying candidates, please contact me at dawn.gullifer@osumc.edu. Dawn Gullifer BS, HT (ASCP) Laboratory Supervisor OSU Histology Lab, LLC 614-293-0358 office 614-293-0345 lab dawn.gullifer@osumc.edu This e-mail, including attachments, may include confidential and/or proprietary information, and may be used only by the person or entity to which it is addressed. If the reader of this e-mail is not the intended recipient or his or her authorized agent, the reader is hereby notified that any dissemination, distribution or copying of this e-mail is prohibited. If you have received this e-mail in error, please notify the sender by responding to this e-mail and destroy immediately. From mary.gessford <@t> spcorp.com Fri Jun 6 13:33:28 2008 From: mary.gessford <@t> spcorp.com (Gessford, Mary) Date: Fri Jun 6 13:33:37 2008 Subject: [Histonet] Sally's Friday Fume In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E636F@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: I don't believe the rules have changed. Unless they changed in the last few weeks. I just sent verification for one of my techs to take the HTL in the last 2 months and had no problems. We are a large Pharma, our lab is all Vet Path. There are at least 21 Vet schools in the US and a State Diagnostic lab in every State in the country. My HT goes back to 1982 and my HTL and IHC are from the 2003 and 2005. The VIR group of the NSH is a large group of Registered techs. We have always been covered under the same ASCP. There is no distinction between human and animal. In fact, when grading the practical (that was discontinued) in the last 10 years or so the ASCP always had a vet path and histo tech from VIR on the grading committee. I believe Liz was a grader. You should call ASCP again and set them strait, maybe I will! Mary Gessford BA,HT,HTL(ASCP)IHC Scientist II Supervisor Anatomic Pathology Schering-Plough Summit, NJ 908-473-4358 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, June 06, 2008 12:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sally's Friday Fume Okay, I finally have something to Fume about. I am beginning to train a person to become a certified histologist. She is a certified veterinary tech with an Associates Degree and the appropriate number of chemistry/biology hours. I inquired of ASCP about her eligibility (after we finish the training period) to take the HT exam. According to ASCP, as a vet diagnostic lab, we do not meet the ASCP requirements for eligibility because we are not accredited by CAP, JCAHO/AABB and do not have a board-certified (read "human") pathologist/medical scientist (hmmm? Could one of our veterinary pathologists fulfill the description of "medical scientist"??). Surely there are other persons working in a veterinary pathology lab that would be interested in certification, one would think. What are they to do? This frosts my petunias and I'd like to know if anyone has any experience or input on this. But - hey! - some of my fluids/solutions in gallon/liter containers seem to have been improved so they don't drip, so maybe someone out there IS listening (that was my first Friday Hour of Fuming subject). Hasta lumbago. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From TCostello <@t> gtxinc.com Fri Jun 6 13:45:00 2008 From: TCostello <@t> gtxinc.com (Terry Costello) Date: Fri Jun 6 13:46:07 2008 Subject: [Histonet] unsubscribe Message-ID: Thanks. Terry Costello Senior Laboratory Animal Technologist GTx, Inc 3rd Floor Van Vleet Bldg. Memphis, TN 38163 900-523-9700 ext. 102 Fax: 901-523-9772 tcostello@gtxinc.com This electronic message, including any attachments, is confidential and proprietary and is solely for the intended recipient. If you are not the intended recipient, this message was sent to you in error and you are hereby advised that any review, disclosure, copying, distribution or use of this message, or any of the information included therein, is unauthorized and strictly prohibited. If you have received this electronic transmission in error, please immediately notify the sender by reply and permanently delete all copies of this message and its attachments. From JMyers1 <@t> aol.com Fri Jun 6 14:00:57 2008 From: JMyers1 <@t> aol.com (JMyers1@aol.com) Date: Fri Jun 6 14:01:04 2008 Subject: [Histonet] Peroxidase blocking in frozen sections Message-ID: 'Netters: I'd like to know what reagents/methods people are using to block for endogenous peroxidase in frozen sections. Any feedback that you might be willing to offer is greatly appreciated. Joe **************Get trade secrets for amazing burgers. Watch "Cooking with Tyler Florence" on AOL Food. (http://food.aol.com/tyler-florence?video=4?&NCID=aolfod00030000000002) From liz <@t> premierlab.com Fri Jun 6 14:05:30 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Jun 6 14:05:37 2008 Subject: [Histonet] Sally's Friday Fume In-Reply-To: References: <4D14F0FC9316DD41972D5F03C070908B017E636F@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: Mary is right, I was a grader and also sat on the BOR for about 8 or so years back in the late 80's and 90's. There was always representation on both the Board of Registry Histotechnology Exam Committee and within the pool of individuals who were slide graders from VIR, both veterinary pathologists, HT's or HTL's that worked in research. It has always been a very balanced group of individuals consisting of techs, veterinary pathologists and MD pathologists. At least when I was invloved. In my situation here, since this is my own lab and there is no medical director or pathologist who could sign we needed to write a letter to the BOR, that's what they told us to do at the time my tech sat for the HTL and when the two of us took the IHC qualification, that was around 2002 or 2003 I think. I'm just guessing but I think that the individual who told you this info was misinformed not unless things have changed. Is there anyone out there who is on the current BOR Histotechnology exam committee comment? I just reviewed the HTL requirements on line an here is what it states, it looks like experience in any histopathology laboratory will do (am I reading this correctly?) so I think that the person you spoke to at ASCP was misinformed. To be eligible for this examination category, an applicant must satisfy the requirements of at least one of the following routes: Route 1: Baccalaureate degree from a regionally accredited college/university with a combination of 30 semester hours (45 quarter hours) of biology and chemistry AND successful completion of a NAACLS accredited Histotechnician or Histotechnology program within the last 5 years; or Route 2: Baccalaureate degree from a regionally accredited college/university with a combination of 30 semester hours (45 quarter hours) of biology and chemistry AND one year full time acceptable experience in a histopathology laboratory in the U.S., Canada or a CAP/The Joint Commission (JCAHO)/AABB accredited laboratory within the last ten years. This year of experience must be under the supervision of a pathologist (certified by the American Board of Pathology in Anatomic Pathology) or an appropriately board certified medical scientist. Clinical Laboratory Experience To fulfill the experience requirement for the Histotechnologist examination, you must have experience, within the last ten years, in the following areas: Fixation Microtomy Processing Staining Thanks Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gessford, Mary Sent: Friday, June 06, 2008 12:33 PM To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Sally's Friday Fume I don't believe the rules have changed. Unless they changed in the last few weeks. I just sent verification for one of my techs to take the HTL in the last 2 months and had no problems. We are a large Pharma, our lab is all Vet Path. There are at least 21 Vet schools in the US and a State Diagnostic lab in every State in the country. My HT goes back to 1982 and my HTL and IHC are from the 2003 and 2005. The VIR group of the NSH is a large group of Registered techs. We have always been covered under the same ASCP. There is no distinction between human and animal. In fact, when grading the practical (that was discontinued) in the last 10 years or so the ASCP always had a vet path and histo tech from VIR on the grading committee. I believe Liz was a grader. You should call ASCP again and set them strait, maybe I will! Mary Gessford BA,HT,HTL(ASCP)IHC Scientist II Supervisor Anatomic Pathology Schering-Plough Summit, NJ 908-473-4358 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, June 06, 2008 12:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sally's Friday Fume Okay, I finally have something to Fume about. I am beginning to train a person to become a certified histologist. She is a certified veterinary tech with an Associates Degree and the appropriate number of chemistry/biology hours. I inquired of ASCP about her eligibility (after we finish the training period) to take the HT exam. According to ASCP, as a vet diagnostic lab, we do not meet the ASCP requirements for eligibility because we are not accredited by CAP, JCAHO/AABB and do not have a board-certified (read "human") pathologist/medical scientist (hmmm? Could one of our veterinary pathologists fulfill the description of "medical scientist"??). Surely there are other persons working in a veterinary pathology lab that would be interested in certification, one would think. What are they to do? This frosts my petunias and I'd like to know if anyone has any experience or input on this. But - hey! - some of my fluids/solutions in gallon/liter containers seem to have been improved so they don't drip, so maybe someone out there IS listening (that was my first Friday Hour of Fuming subject). Hasta lumbago. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Fri Jun 6 14:29:28 2008 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Fri Jun 6 14:27:42 2008 Subject: [Histonet] RE: Veterinary Histologist Certification Eligibility In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E6370@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B017E6370@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: Hi Sara, I certainly understand your frustration and I'm in agreement with those who've responded to your original message that the very last thing we wish to do is discourage individuals who wish to become certified. I am copying Marilyn Gamble who is the NSH representative to the Board of Registry so that she can clarify if there has been any misunderstanding during this discussion on Histonet and likewise can represent the issue to the BOR. In the days when the practical was in place, it may have been appropriate for the technician/technologist to receive guidance for the selection of appropriate tissues to satisfy the exam requirements. However, I seem to recall grading slides on animal tissues which at the time I imagined came from veterinary labs. Marilyn please "reply to all" if you can provide information that will clarify the situation. I am forwarding the earlier messages to you separately. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, June 06, 2008 2:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Veterinary Histologist Certification Eligibility That's a mouthful! Anyway - thank you to those who responded to me. I searched out a board-certified pathologist (non-veterinary) who works in our building (we share a State of NM building with OMI and the State Laboratory) and he was as astounded as I. I appreciate the feedback about writing a letter to BOR requesting a "waiver" for a veterinary situation and I will try that when the time comes. Our lab, in anticipation of our new building (2010) is operating under conditional AALVD certification but certainly not under CAP/JCHAO. I'm thinking of asking for a definition of "medical scientist" from the ASCP. The saga continues, but "where there's a will, there's a way". One would think anyone who is interested in becoming a certified histologist would receive the maximum amount of encouragement, from the employer AND the certifying agency. Perhaps it's time for a review of the requirements or at least of the definition of an employer's validity to provide appropriate training, hmmm??? Mr. Della Speranza, do you have a take on this? It is not my goal here to fluster, annoy, or pester. It's just that I'm beginning to see a certain amount of reluctance on the part of those institutions whose interest should be to encourage the growth of our profession. Now that my aim is to interest local mid-high and high school forensic science students in a detour to the Paraffin Side, I have a path littered with roadblocks. If our profession had the same PR firm that the anti-tobacco industry had, we'd be up to our earlobes in histotechs (did that come out right?). Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SohrabB1 <@t> ah.org Fri Jun 6 14:49:26 2008 From: SohrabB1 <@t> ah.org (Behnaz Sohrab) Date: Fri Jun 6 14:50:10 2008 Subject: [Histonet] SLide Holder Message-ID: <48493246.4347.0054.0@ah.org> Any one knows where I can buy brown/black microwave able slide holder? Thanks,Behnaz Sohrab From ploykasek <@t> phenopath.com Fri Jun 6 15:01:03 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri Jun 6 15:01:15 2008 Subject: [Histonet] IHC neg. controls (Friday rant) Message-ID: Can someone rationalize to me the practice of running a negative control for every antibody in an IHC workup? For example, six antibodies & six negative controls??? This makes me crazy. Just had a case, needle biopsy, where this occurred at an outside institution, and now we don?t have enough tumor left to run more IHC & get a diagnosis. It borders on malpractice IMO. On the AP CAP checklist ANP.2270 Are appropriate negative controls used? The comment has the following (near the end): A negative tissue control must be processed for each antibody in a given run. Any of the following can serve as a negative tissue control: 1. Multitissue blocks. These can provide simultaneous positive and negative tissue controls, and are considered ?best practice? 2. The positive control slide or patient test slides, if these slides contain tissue elements that should not react with the antibody. 3. A separate negative tissue control slide. I think it best to asses this negative tissue control on your positive controls that should contain negative elements. Plus, use known negative elements on the patient slides. The patient tissue is precious & these patients have undergone procedures that have associated morbidity. I can?t see using up patient tissue for multiple negative controls & Having the patient have to undergo another procedure! Ok ? I?ll stop now. Patti Loykasek This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From ploykasek <@t> phenopath.com Fri Jun 6 15:49:42 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri Jun 6 15:49:55 2008 Subject: [Histonet] IHC neg. controls (Friday rant) In-Reply-To: <6BFF6D137DF6BC43B33891BA96E83B19018AA2D5@PGHCR-EXMB-VS-1.na.fshrnet.com> Message-ID: Hi Tim. I totally agree with you. I DO NOT think a negative should be run for each antibody. In the comment of the ANP.2270 they even mention running one for each antigen retrieval, but add if not then to use most aggressive AR & they list the AR in order of aggresiveness. Running a negative for each antibody is waste & if cited by a CAP inspector, I would appeal to CAP - especially considering the way the comment is written. I was hoping someone who is running a negative with each antibody could tell me their rationale for doing this. Patti > Patti, It does not say you need a separate negative tissue for each antibody, > only "A" negative control tissue. One of each block, and for each protocol > (ie, different pretreatments or detection system) would suffice. > > > Tim Morken > Technical Support Manager > Lab Vision Products > Anatomical Pathology > ThermoFisher Scientific > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek > Sent: Friday, June 06, 2008 1:01 PM > To: histonet > Subject: [Histonet] IHC neg. controls (Friday rant) > > Can someone rationalize to me the practice of running a negative control for > every antibody in an IHC workup? For example, six antibodies & six negative > controls??? This makes me crazy. Just had a case, needle biopsy, where this > occurred at an outside institution, and now we don?t have enough tumor left to > run more IHC & get a diagnosis. It borders on malpractice IMO. > > On the AP CAP checklist ANP.2270 Are appropriate negative controls used? The > comment has the following (near the end): > > A negative tissue control must be processed for each antibody in a given run. > Any of the following can serve as a negative tissue control: > > 1. Multitissue blocks. These can provide simultaneous positive > and negative tissue controls, and are considered ?best practice? > 2. The positive control slide or patient test slides, if these > slides contain tissue elements that should not react with the antibody. > 3. A separate negative tissue control slide. > > I think it best to asses this negative tissue control on your positive > controls that should contain negative elements. Plus, use known negative > elements on the patient slides. The patient tissue is precious & these > patients have undergone procedures that have associated morbidity. I can?t see > using up patient tissue for multiple negative controls & Having the patient > have to undergo another procedure! > Ok ? I?ll stop now. > > Patti Loykasek > > > This e-mail message, including any attachments, is for the sole use of the > intended recipients and may contain privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not the > intended recipient, please contact the sender by e-mail and destroy all copies > of the original message, or you may call PhenoPath Laboratories, Seattle, WA > U.S.A. > at (206) 374-9000. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From slappycraw <@t> yahoo.com Fri Jun 6 16:04:57 2008 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Fri Jun 6 16:05:01 2008 Subject: [Histonet] IHC neg. controls (Friday rant) In-Reply-To: Message-ID: <806964.24310.qm@web53606.mail.re2.yahoo.com> There could be a legal angle to it in which case wouldn't make it rational but that is the only thing I can come up with on a Friday. Patti Loykasek wrote: Can someone rationalize to me the practice of running a negative control for every antibody in an IHC workup? For example, six antibodies & six negative controls??? This makes me crazy. Just had a case, needle biopsy, where this occurred at an outside institution, and now we don?t have enough tumor left to run more IHC & get a diagnosis. It borders on malpractice IMO. On the AP CAP checklist ANP.2270 Are appropriate negative controls used? The comment has the following (near the end): A negative tissue control must be processed for each antibody in a given run. Any of the following can serve as a negative tissue control: 1. Multitissue blocks. These can provide simultaneous positive and negative tissue controls, and are considered ?best practice? 2. The positive control slide or patient test slides, if these slides contain tissue elements that should not react with the antibody. 3. A separate negative tissue control slide. I think it best to asses this negative tissue control on your positive controls that should contain negative elements. Plus, use known negative elements on the patient slides. The patient tissue is precious & these patients have undergone procedures that have associated morbidity. I can?t see using up patient tissue for multiple negative controls & Having the patient have to undergo another procedure! Ok ­ I?ll stop now. Patti Loykasek This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Larry A. Woody Seattle, Wa. From rjbuesa <@t> yahoo.com Fri Jun 6 16:07:43 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 6 16:07:48 2008 Subject: [Histonet] IHC neg. controls (Friday rant) In-Reply-To: Message-ID: <23683.58242.qm@web65707.mail.ac4.yahoo.com> If you detection system is the same and all the procedure is the same for the 6 Abs, except for the Abs, you will need only ONE negative control per tissue, per block, but not per Ab. Ren? J. Patti Loykasek wrote: Can someone rationalize to me the practice of running a negative control for every antibody in an IHC workup? For example, six antibodies & six negative controls??? This makes me crazy. Just had a case, needle biopsy, where this occurred at an outside institution, and now we don?t have enough tumor left to run more IHC & get a diagnosis. It borders on malpractice IMO. On the AP CAP checklist ANP.2270 Are appropriate negative controls used? The comment has the following (near the end): A negative tissue control must be processed for each antibody in a given run. Any of the following can serve as a negative tissue control: 1. Multitissue blocks. These can provide simultaneous positive and negative tissue controls, and are considered ?best practice? 2. The positive control slide or patient test slides, if these slides contain tissue elements that should not react with the antibody. 3. A separate negative tissue control slide. I think it best to asses this negative tissue control on your positive controls that should contain negative elements. Plus, use known negative elements on the patient slides. The patient tissue is precious & these patients have undergone procedures that have associated morbidity. I can?t see using up patient tissue for multiple negative controls & Having the patient have to undergo another procedure! Ok ­ I?ll stop now. Patti Loykasek This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rosenfeldtek <@t> hotmail.com Fri Jun 6 17:40:19 2008 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Fri Jun 6 17:40:23 2008 Subject: [Histonet] RE:Peroxidase blocking in frozen sections In-Reply-To: References: Message-ID: I use Zymed's Peroxo-Block for 30-45 seconds. Jerry Ricks Research Scientist University of Washington Department of Pathology> From: JMyers1@aol.com> Date: Fri, 6 Jun 2008 15:00:57 -0400> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] Peroxidase blocking in frozen sections> > 'Netters:> I'd like to know what reagents/methods people are using to block for > endogenous peroxidase in frozen sections. Any feedback that you might be willing to > offer is greatly appreciated.> Joe> > > > **************Get trade secrets for amazing burgers. Watch "Cooking with > Tyler Florence" on AOL Food. > (http://food.aol.com/tyler-florence?video=4?&NCID=aolfod00030000000002)> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ It?s easy to add contacts from Facebook and other social sites through Windows Live? Messenger. Learn how. https://www.invite2messenger.net/im/?source=TXT_EML_WLH_LearnHow From pruegg <@t> ihctech.net Fri Jun 6 20:13:43 2008 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Fri Jun 6 20:13:51 2008 Subject: [Histonet] IHC neg. controls (Friday rant) In-Reply-To: References: Message-ID: <1149.74.127.102.244.1212801223.squirrel@webmail.ihctech.net> Patti et al, this has been a bone of contention in IHC for some time now, the IHC Resource Group has discussed this year after year with no viable solution that CAP will go for, i know for a fact that many in the business save precious tissue and run just one negative reagent control for the antibody species and most agressive pretreatment for the batch, then take their chances with CAP when the time comes. Patsy > Can someone rationalize to me the practice of running a negative control > for > every antibody in an IHC workup? For example, six antibodies & six > negative > controls??? This makes me crazy. Just had a case, needle biopsy, where > this > occurred at an outside institution, and now we don?t have enough tumor > left > to run more IHC & get a diagnosis. It borders on malpractice IMO. > > On the AP CAP checklist ANP.2270 Are appropriate negative controls used? > The > comment has the following (near the end): > > A negative tissue control must be processed for each antibody in a given > run. Any of the following can serve as a negative tissue control: > > 1. Multitissue blocks. These can provide simultaneous > positive > and negative tissue controls, and are considered ?best practice? > 2. The positive control slide or patient test slides, if these > slides contain tissue elements that should not react with the antibody. > 3. A separate negative tissue control slide. > > I think it best to asses this negative tissue control on your positive > controls that should contain negative elements. Plus, use known negative > elements on the patient slides. The patient tissue is precious & these > patients have undergone procedures that have associated morbidity. I can?t > see using up patient tissue for multiple negative controls & Having the > patient have to undergo another procedure! > Ok ? I?ll stop now. > > Patti Loykasek > > > This e-mail message, including any attachments, is for the sole use of the > intended recipients and may contain privileged information. Any > unauthorized > review, use, disclosure or distribution is prohibited. If you are not the > intended > recipient, please contact the sender by e-mail and destroy all copies of > the > original message, or you may call PhenoPath Laboratories, Seattle, WA > U.S.A. > at (206) 374-9000. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From anh2006 <@t> med.cornell.edu Fri Jun 6 20:14:38 2008 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Fri Jun 6 20:14:49 2008 Subject: [Histonet] pAKT Message-ID: What Abs are people using for pAKT these days? The one I had working so well in FFPE is no longer being made by Cell Signaling. Thanks, Andrea -- From pruegg <@t> ihctech.net Fri Jun 6 20:21:08 2008 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Fri Jun 6 20:21:16 2008 Subject: [Histonet] pAKT In-Reply-To: References: Message-ID: <1164.74.127.102.244.1212801668.squirrel@webmail.ihctech.net> cell signaling has a couple of alternative abs for pAKT they claim are better, i believe they are rab monoclonals, i have not tried them yet. Patsy > What Abs are people using for pAKT these days? The one I had working > so well in FFPE is no longer being made by Cell Signaling. > > Thanks, > Andrea > -- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jmaass <@t> frii.com Fri Jun 6 20:56:13 2008 From: jmaass <@t> frii.com (Janet Maass) Date: Fri Jun 6 20:56:17 2008 Subject: [Histonet] Sally's Friday Fume References: <4D14F0FC9316DD41972D5F03C070908B017E636F@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <004201c8c841$aaba1390$0200a8c0@Janet03b999f> Hi Sara I think this was an error on the part of the individual that you communicated with at ASCP. While I served on the HT/HTL Committee for two terms, I worked in veterinary histology. Also on The Committee was a veterinary pathologist for two terms. While owning my own veterinary histology consulting lab, I trained several people who took there certification exam. Currently on The Committee is someone that is working in veterinary histology. Janet Maass From Allison_Scott <@t> hchd.tmc.edu Sat Jun 7 09:27:18 2008 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Sat Jun 7 09:27:23 2008 Subject: [Histonet] Disposing of used paraffin from the tissue processor Message-ID: <1872B4A455B7974391609AD8034C79FC082E1D@LBEXCH01.hchd.local> Hello to all in histoland. How are other facilities disposing of used paraffin from the tissue processor. I have a box that I have lined with several red biohazrd bag and I pour all of the used paraffin in it. I let it harden, and after about a week I pull out the hard paraffin and put it in a biohazard box that has a biohazard bag in it also, close it up and it is ready for our environmental services people to pick up and discard. I would not think that this paraffin can be put into the regular trash since tissues and chemicals have been through it. Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From rjbuesa <@t> yahoo.com Sat Jun 7 10:44:39 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Jun 7 10:44:45 2008 Subject: [Histonet] Disposing of used paraffin from the tissue processor In-Reply-To: <1872B4A455B7974391609AD8034C79FC082E1D@LBEXCH01.hchd.local> Message-ID: <136676.61962.qm@web65702.mail.ac4.yahoo.com> We used to incinerate it. Ren? J. "Scott, Allison D" wrote: Hello to all in histoland. How are other facilities disposing of used paraffin from the tissue processor. I have a box that I have lined with several red biohazrd bag and I pour all of the used paraffin in it. I let it harden, and after about a week I pull out the hard paraffin and put it in a biohazard box that has a biohazard bag in it also, close it up and it is ready for our environmental services people to pick up and discard. I would not think that this paraffin can be put into the regular trash since tissues and chemicals have been through it. Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lmdee1 <@t> yahoo.com Sat Jun 7 11:53:23 2008 From: lmdee1 <@t> yahoo.com (Linda) Date: Sat Jun 7 11:53:31 2008 Subject: [Histonet] NSH Histology Task Analysis and StandardsofPerformance Competen cy Message-ID: <30657.26774.qm@web36503.mail.mud.yahoo.com> I would be most greatfull for a copy too. Linda Dee Chicago, IL ----- Original Message ---- From: Norm Burnham To: "Rathborne, Toni" ; "Mahoney,Janice A" ; "McKnight, Tanisha" ; histonet@lists.utsouthwestern.edu Sent: Friday, June 6, 2008 10:48:33 AM Subject: RE: [Histonet] NSH Histology Task Analysis and StandardsofPerformance Competen cy Me too!? Norm Burnham -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, June 06, 2008 10:27 AM To: Mahoney,Janice A; McKnight, Tanisha; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH Histology Task Analysis and StandardsofPerformance Competen cy As would I. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Mahoney,Janice A Sent: Friday, June 06, 2008 11:23 AM To: 'McKnight, Tanisha'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH Histology Task Analysis and Standards ofPerformance Competen cy I'd love to have an electronic copy as well Jan Mahoney Alegent Health Omaha,NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McKnight, Tanisha Sent: Friday, June 06, 2008 10:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NSH Histology Task Analysis and Standards of Performance Competen cy Hello Histo-netters: Would anyone happen to have an electronic copy of NSH Histology Task Analysis and The Standards of Performance/Competency that you could send to me? I am in desperate need of these items. I've contacted NSH for these twice, but I have gotten no response. I'd greatly appreciate anything you could send. Tanisha N. McKnight, HT (ASCP) Covance CLS Indianapolis Specimen Management, Anatomic Pathology ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful.? If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer.? Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening.? Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail.? Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law.? Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.? If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. ______________________________________________________________________________ This e-mail may contain confidential or privileged information. If you think you have received this e-mail in error, please advise the sender by reply e-mail and then delete this e-mail immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lynnd01 <@t> hotmail.com Sat Jun 7 16:39:09 2008 From: lynnd01 <@t> hotmail.com (Lynn Dike) Date: Sat Jun 7 16:39:17 2008 Subject: [Histonet] NSH Histology Task Analysis and StandardsofPerformance Competen cy In-Reply-To: <30657.26774.qm@web36503.mail.mud.yahoo.com> References: <30657.26774.qm@web36503.mail.mud.yahoo.com> Message-ID: Just had our CAP Inspection and it was our only deficiency.....would you sen it to me also. Thany you Lynn> Date: Sat, 7 Jun 2008 09:53:23 -0700> From: lmdee1@yahoo.com> To: Norm.Burnham@propath.com; trathborne@somerset-healthcare.com; JMahoney@alegent.org; tanisha.mcknight@covance.com; histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] NSH Histology Task Analysis and StandardsofPerformance Competen cy> CC: > > I would be most greatfull for a copy too.> Linda Dee> Chicago, IL> > > > ----- Original Message ----> From: Norm Burnham > To: "Rathborne, Toni" ; "Mahoney,Janice A" ; "McKnight, Tanisha" ; histonet@lists.utsouthwestern.edu> Sent: Friday, June 6, 2008 10:48:33 AM> Subject: RE: [Histonet] NSH Histology Task Analysis and StandardsofPerformance Competen cy> > Me too! > > Norm Burnham> > -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne,> Toni> Sent: Friday, June 06, 2008 10:27 AM> To: Mahoney,Janice A; McKnight, Tanisha; histonet@lists.utsouthwestern.edu> Subject: RE: [Histonet] NSH Histology Task Analysis and> StandardsofPerformance Competen cy> > As would I.> > > > -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu> [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of> Mahoney,Janice A> Sent: Friday, June 06, 2008 11:23 AM> To: 'McKnight, Tanisha'; histonet@lists.utsouthwestern.edu> Subject: RE: [Histonet] NSH Histology Task Analysis and Standards> ofPerformance Competen cy> > > I'd love to have an electronic copy as well> Jan Mahoney> Alegent Health> Omaha,NE> > -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McKnight,> Tanisha> Sent: Friday, June 06, 2008 10:08 AM> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] NSH Histology Task Analysis and Standards of Performance> Competen cy> > Hello Histo-netters:> > Would anyone happen to have an electronic copy of NSH Histology Task> Analysis and The Standards of Performance/Competency that you could send> to me? I am in desperate need of these items. I've contacted NSH for> these twice, but I have gotten no response.> > I'd greatly appreciate anything you could send.> > Tanisha N. McKnight, HT (ASCP)> Covance CLS Indianapolis> Specimen Management, Anatomic Pathology> > > > > > -----------------------------------------------------> Confidentiality Notice: This e-mail transmission> may contain confidential or legally privileged> information that is intended only for the individual> or entity named in the e-mail address. If you are not> the intended recipient, you are hereby notified that> any disclosure, copying, distribution, or reliance> upon the contents of this e-mail is strictly prohibited.> > If you have received this e-mail transmission in error,> please reply to the sender, so that we can arrange> for proper delivery, and then please delete the message> from your inbox. Thank you.> > > Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent> Health is faithful to the healing ministry of Jesus Christ, providing high> quality care for the body, mind and spirit of every person.> > The information contained in this communication, including attachments, is> confidential and private and intended only for the use of the addressees.> Unauthorized use, disclosure, distribution or copying is strictly prohibited> and may be unlawful. If you received this communication in error, please> inform us of the erroneous delivery by return e-mail message from your> computer. Additionally, although all attachments have been scanned at the> source for viruses, the recipient should check any attachments for the> presence of viruses before opening. Alegent Health accepts no liability for> any damage caused by any virus transmitted by this e-mail. Thank you for> your cooperation.> > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE> This message and any included attachments are from Somerset Medical Center> and are intended only for the addressee. The information contained in this> message is confidential and may contain privileged, confidential,> proprietary and/or trade secret information entitled to protection and/or> exemption from disclosure under applicable law. Unauthorized forwarding,> printing, copying, distribution, or use of such information is strictly> prohibited and may be unlawful. If you are not the addressee, please> promptly delete this message and notify the sender of the delivery error> by e-mail or you may call Somerset Medical Center's computer Help Desk> at 908-685-2200, ext. 4050.> > Somerset Medical Center is proud to receive the Somerset County > Business Partnership's 2006 Quality of Life Award.> ______________________________________________________________________________> > > This e-mail may contain confidential or privileged information. If you think you have received this e-mail > in error, please advise the sender by reply e-mail and then delete this e-mail immediately.> > > > > > > > > > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Now you can invite friends from Facebook and other groups to join you on Windows Live? Messenger. Add now. https://www.invite2messenger.net/im/?source=TXT_EML_WLH_AddNow_Now From thisisann <@t> aol.com Sat Jun 7 20:01:23 2008 From: thisisann <@t> aol.com (thisisann@aol.com) Date: Sat Jun 7 20:01:30 2008 Subject: [Histonet] Control Tissue Message-ID: <8CA971A4E1ED717-1734-F20@FWM-D28.sysops.aol.com> Does anyone know where I can get control tissue.? I know I can purchase slides, but is there a website that I can go to to request control tissue at no cost, tissue such as tonsil, etc. that a hospital is discarding? Thanks, Ann From suriana <@t> spdscientific.com.sg Sun Jun 8 19:40:52 2008 From: suriana <@t> spdscientific.com.sg (Suriana Bte Abdul Latiff) Date: Sun Jun 8 19:41:08 2008 Subject: [Histonet] Feedback on tissue arrayer, please? In-Reply-To: Message-ID: <2EF74098586A7B44B45162438E0F3CD2729329@Lithium.LABGISTICS.COM> Should you want to get an arrayer.. I suggest the semi automated expert called Minicore: http://www.alphelys.com/site/index_us.php Sue -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C. Sent: Friday, June 06, 2008 2:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Feedback on tissue arrayer, please? <> Dear Histonetters, If any of you have tried this tissue arrayer, could you please share your impressions? The video describing the use of this product is only 3-5 minutes. It looks very impressive! Shortcut to: http://arraymold.com/order.html From suriana <@t> spdscientific.com.sg Sun Jun 8 20:06:23 2008 From: suriana <@t> spdscientific.com.sg (Suriana Bte Abdul Latiff) Date: Sun Jun 8 20:06:30 2008 Subject: [Histonet] Dako Autostainer In-Reply-To: Message-ID: <2EF74098586A7B44B45162438E0F3CD272935A@Lithium.LABGISTICS.COM> Most likely the probe needs to be changed.. it has become less sensitive. Regards Sue -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DeMatteo, Gabriela Sent: Friday, June 06, 2008 11:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dako Autostainer About the same problem, beside the sensor malfunction, could the software be the problem? Our "immuno specialist" said that the service tech found nothing wrong with the Dako instrument. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Sun Jun 8 21:06:31 2008 From: shive003 <@t> umn.edu (shive003@umn.edu) Date: Sun Jun 8 21:06:35 2008 Subject: [Histonet] IHC neg. controls (Friday rant) In-Reply-To: <23683.58242.qm@web65707.mail.ac4.yahoo.com> References: <23683.58242.qm@web65707.mail.ac4.yahoo.com> Message-ID: I agree with Rene'... for instance, if you're running three different cytokeratins on your case tissue, all mouse monoclonals, all requiring enzyme digestion for unmasking antigens, all with the same detection system and protocols... why run 3 separate negative controls on the same block? They'll be identical copies of each other. All you're doing is wasting reagents, supplies, and precious tissue. One negative control per block per pretreatment and/or detection system. More than that is just illogical to me. Jan Shivers On Jun 6 2008, Rene J Buesa wrote: > If you detection system is the same and all the procedure is the same for > the 6 Abs, except for the Abs, you will need only ONE negative control > per tissue, per block, but not per Ab. > Ren? J. > >Patti Loykasek wrote: > Can someone rationalize to me the practice of running a negative > control for every antibody in an IHC workup? For example, six antibodies > & six negative controls??? This makes me crazy. Just had a case, needle > biopsy, where this occurred at an outside institution, and now we don?t > have enough tumor left to run more IHC & get a diagnosis. It borders on > malpractice IMO. > > On the AP CAP checklist ANP.2270 Are appropriate negative controls used? > The comment has the following (near the end): > >A negative tissue control must be processed for each antibody in a given >run. Any of the following can serve as a negative tissue control: > >1. Multitissue blocks. These can provide simultaneous positive >and negative tissue controls, and are considered ?best practice? >2. The positive control slide or patient test slides, if these >slides contain tissue elements that should not react with the antibody. >3. A separate negative tissue control slide. > >I think it best to asses this negative tissue control on your positive >controls that should contain negative elements. Plus, use known negative >elements on the patient slides. The patient tissue is precious & these >patients have undergone procedures that have associated morbidity. I can?t >see using up patient tissue for multiple negative controls & Having the >patient have to undergo another procedure! >Ok ­ I?ll stop now. > >Patti Loykasek > > > This e-mail message, including any attachments, is for the sole use of > the intended recipients and may contain privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If > you are not the intended recipient, please contact the sender by e-mail > and destroy all copies of the original message, or you may call PhenoPath > Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > _______________________________________________ Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From dspears <@t> mmci.org Mon Jun 9 07:20:04 2008 From: dspears <@t> mmci.org (Dana Spears) Date: Mon Jun 9 07:20:36 2008 Subject: [Histonet] NSH Histology Task Analysis and StandardsofPerformance Competen cy Message-ID: Please send to me as well! Thank you! Dana Spears, HTL(ASCP) Anatomic Pathology Manager Methodist Medical Center (309) 672-4930 (office) (309) 255-7214 (cell) dspears@mmci.org >>> Lynn Dike 6/7/2008 4:39 PM >>> Just had our CAP Inspection and it was our only deficiency.....would you sen it to me also. Thany you Lynn> Date: Sat, 7 Jun 2008 09:53:23 -0700> From: lmdee1@yahoo.com> To: Norm.Burnham@propath.com; trathborne@somerset-healthcare.com; JMahoney@alegent.org; tanisha.mcknight@covance.com; histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] NSH Histology Task Analysis and StandardsofPerformance Competen cy> CC: > > I would be most greatfull for a copy too.> Linda Dee> Chicago, IL> > > > ----- Original Message ----> From: Norm Burnham > To: "Rathborne, Toni" ; "Mahoney,Janice A" ; "McKnight, Tanisha" ; histonet@lists.utsouthwestern.edu> Sent: Friday, June 6, 2008 10:48:33 AM> Subject: RE: [Histonet] NSH Histology Task Analysis and StandardsofPerformance Competen cy> > Me too! > > Norm Burnham> > -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne,> Toni> Sent: Friday, June 06, 2008 10:27 AM> To: Mahoney,Janice A; McKnight, Tanisha; histonet@lists.utsouthwestern.edu> Subject: RE: [Histonet] NSH Histology Task Analysis and> StandardsofPerformance Competen cy> > As would I.> > > > -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu> [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of> Mahoney,Janice A> Sent: Friday, June 06, 2008 11:23 AM> To: 'McKnight, Tanisha'; histonet@lists.utsouthwestern.edu> Subject: RE: [Histonet] NSH Histology Task Analysis and Standards> ofPerformance Competen cy> > > I'd love to have an electronic copy as well> Jan Mahoney> Alegent Health> Omaha,NE> > -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McKnight,> Tanisha> Sent: Friday, June 06, 2008 10:08 AM> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] NSH Histology Task Analysis and Standards of Performance> Competen cy> > Hello Histo-netters:> > Would anyone happen to have an electronic copy of NSH Histology Task> Analysis and The Standards of Performance/Competency that you could send> to me? I am in desperate need of these items. I've contacted NSH for> these twice, but I have gotten no response.> > I'd greatly appreciate anything you could send.> > Tanisha N. McKnight, HT (ASCP)> Covance CLS Indianapolis> Specimen Management, Anatomic Pathology> > > > > > -----------------------------------------------------> Confidentiality Notice: This e-mail transmission> may contain confidential or legally privileged> information that is intended only for the individual> or entity named in the e-mail address. If you are not> the intended recipient, you are hereby notified that> any disclosure, copying, distribution, or reliance> upon the contents of this e-mail is strictly prohibited.> > If you have received this e-mail transmission in error,> please reply to the sender, so that we can arrange> for proper delivery, and then please delete the message> from your inbox. Thank you.> > > Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent> Health is faithful to the healing ministry of Jesus Christ, providing high> quality care for the body, mind and spirit of every person.> > The information contained in this communication, including attachments, is> confidential and private and intended only for the use of the addressees.> Unauthorized use, disclosure, distribution or copying is strictly prohibited> and may be unlawful. If you received this communication in error, please> inform us of the erroneous delivery by return e-mail message from your> computer. Additionally, although all attachments have been scanned at the> source for viruses, the recipient should check any attachments for the> presence of viruses before opening. Alegent Health accepts no liability for> any damage caused by any virus transmitted by this e-mail. Thank you for> your cooperation.> > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE> This message and any included attachments are from Somerset Medical Center> and are intended only for the addressee. The information contained in this> message is confidential and may contain privileged, confidential,> proprietary and/or trade secret information entitled to protection and/or> exemption from disclosure under applicable law. Unauthorized forwarding,> printing, copying, distribution, or use of such information is strictly> prohibited and may be unlawful. If you are not the addressee, please> promptly delete this message and notify the sender of the delivery error> by e-mail or you may call Somerset Medical Center's computer Help Desk> at 908-685-2200, ext. 4050.> > Somerset Medical Center is proud to receive the Somerset County > Business Partnership's 2006 Quality of Life Award.> ______________________________________________________________________________> > > This e-mail may contain confidential or privileged information. If you think you have received this e-mail > in error, please advise the sender by reply e-mail and then delete this e-mail immediately.> > > > > > > > > > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Now you can invite friends from Facebook and other groups to join you on Windows Live* Messenger. Add now. https://www.invite2messenger.net/im/?source=TXT_EML_WLH_AddNow_Now_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE: This message is a PRIVATE communication. This e-mail may contain confidential or proprietary information that may be considered legally privileged. It is intended only for the named recipient(s). If an addressing or transmission error has misdirected the e-mail, please notify the author by replying to this message. If you are not the named recipient, you are not authorized to use, disclose, distribute, copy, print, or rely on this e-mail, and should immediately delete it from your computer system. Thank you for your assistance with this matter. From sbreeden <@t> nmda.nmsu.edu Mon Jun 9 07:31:05 2008 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Mon Jun 9 07:31:10 2008 Subject: [Histonet] Vet Lab Histology Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E6379@nmdamailsvr.nmda.ad.nmsu.edu> Thank you to everyone who wrote with comments and clarification about the eligibility for certification of a trainee in a veterinary diagnostic lab. I had phoned the ASCP and asked this question and was sent a quote from their website regarding eligibility; since it was well-defined, I thought there would be no recourse. Perhaps it's time for a revision of the paragraph regarding eligibility on the ASCP website... Happy Monday! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From godsgalnow <@t> aol.com Mon Jun 9 07:46:56 2008 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Mon Jun 9 07:47:08 2008 Subject: [Histonet] Her 2 FISH/PCA3/DASL Message-ID: <8CA984608CC4BAA-1160-1630@FWM-D42.sysops.aol.com> Of the labs of there performing this test, are you doing it as part of your histology lab, or does this go to another department?? What about PCA3?? UroVysion? Anyone doing DASL by Illumina?? Same questions as above, please. Thanks in advance, Roxanne From relia1 <@t> earthlink.net Mon Jun 9 07:57:04 2008 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Jun 9 07:57:07 2008 Subject: [Histonet] RELIA Histology Spotlight Opportunity 6-9-08 Message-ID: Hello Histonetters!! How are you? Are you ready to start the summer? I just wanted to make you aware of this opportunity that I feel is a really great one. Just in case you might be interested or know someone who might be interested I am sending this special bulletin out. The reasons that I feel that this is a special opportunity are because 2 candidates placed here by RELIA endorse it as a great place to work and live. Also there is the opportunity to learn new things like electron microscopy. This is the posting that I put on the internet about this position. If you or someone you know might be interested please contact me or have them contact me at relia1@earthlink.net or toll free at 866-607-3542. RELIA the nation's only permanent placement firm dedicated solely to the histology profession is assisting an exciting Research company with the search for a histology technician or technologist who is ASCP eligible. This client offers excellent salary benefits and relocation assistance. 2 candidates placed here by RELIA endorse it as a great place to work and live. Here is the job description: The Histology Technician/Histotechnologist will be responsible for processing, sectioning, staining, and preparing slides of tissue samples for microscopic evaluation by pathologists. Responsibilities include: Maintain Good Laboratory Practices (GLP). Process, embed, section, and perform necessary stains to highlight specific tissue patterns and structures. Perform quality control (QC) on finished slide preparations. Maintain and troubleshoot equipment operation failures; take appropriate actions to keep interruptions in work flow to a minimum. Execute special histological and histochemical stains used to emphasize specific tissue elements. Perform special histological procedures such as frozen sectioning and electron microscopy preparations (e.g. plastic processing, embedding, sectioning), and photomicrography. Requirements include 2 years current experience in histology or an Associate?s (AA) degree or higher, specializing in anatomy-related medical science, plus at least 1 year related experience and/or training. ASCP HT/HTL or eligible. For more information contact Pam Barker at RELIA Solutions. e-mail: relia1@earthlink.net or toll free at 866-60-RELIA (866-607-3542) RELIA Solutions is the only nationwide recruiting firm specializing in the permanent placement of histology professionals Thanks for taking the time to take a look!! Have a great day!! Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.myspace.com/pamatrelia From rjr6 <@t> psu.edu Mon Jun 9 08:13:56 2008 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Mon Jun 9 08:14:04 2008 Subject: [Histonet] RE: Sally's Friday Fume In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E636F@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B017E636F@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: I work in a veterinary diagnostic lab and took the HTL exam in 2000. I had no problem with eligibility. We have board certified veterinary pathologists here. We even used animal tissue for most of the practical although I did have to beg the hospital for some appendix. Roberta Horner HT/HTL Penn State University Animal Diagnostic Lab -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, June 06, 2008 12:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sally's Friday Fume Okay, I finally have something to Fume about. I am beginning to train a person to become a certified histologist. She is a certified veterinary tech with an Associates Degree and the appropriate number of chemistry/biology hours. I inquired of ASCP about her eligibility (after we finish the training period) to take the HT exam. According to ASCP, as a vet diagnostic lab, we do not meet the ASCP requirements for eligibility because we are not accredited by CAP, JCAHO/AABB and do not have a board-certified (read "human") pathologist/medical scientist (hmmm? Could one of our veterinary pathologists fulfill the description of "medical scientist"??). Surely there are other persons working in a veterinary pathology lab that would be interested in certification, one would think. What are they to do? This frosts my petunias and I'd like to know if anyone has any experience or input on this. But - hey! - some of my fluids/solutions in gallon/liter containers seem to have been improved so they don't drip, so maybe someone out there IS listening (that was my first Friday Hour of Fuming subject). Hasta lumbago. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bakevictoria <@t> gmail.com Mon Jun 9 08:28:24 2008 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Mon Jun 9 08:28:28 2008 Subject: [Histonet] Control Tissue In-Reply-To: <8CA971A4E1ED717-1734-F20@FWM-D28.sysops.aol.com> References: <8CA971A4E1ED717-1734-F20@FWM-D28.sysops.aol.com> Message-ID: <4f016b690806090628ye36570dl56629dfa3753acb5@mail.gmail.com> Ann, Last time I tried to do this it was a real 'sticky wicket' because of the HIPPA guidelines. In my case it was for research and it took me a lot of wrangling to get it. It used to be that you could purchase tissue blocks from vendors who would obtain them from hospital mortuary or signed out cases. I even tried once to see if I could get one of the vendors who sell the cut slides to sell my lab a block and they would not sighting HIPPA and a few other rules/laws possibly $$'s. The CDC used to have certain tissue blocks available years ago, but that has also stopped. Not knowing what type of lab (reference, research - essentially non-hospital) you work in I only have a couple of options that I can think of. I got the help of a pathologist and my Director of Research to reach out to sites that might be willing to help us with obtaining tissue for control material but we had to sign documentation that it would only be used for research purposes etc and we paid a 'donation' to them for use in teaching at their site. If you do work for hospitals in your lab, possibly you and a trusted pathologist could contact one or two of them to see if they could help you. I disliked having to do all this cloak n' dagger nonsense to get the tissue I needed, but it was the only way I could think of at the time. Also, what about ASCP/NSH/State chapters. Maybe someone has a contact that can help you as well. As to getting it at no cost, I have no idea if this is possible anymore. You're caught between the perpetual rock and hard place with this. You need the tissue to perform the test to help dx the patient, but guidelines prevent you from getting the materials you need to do that test. Vikki On 6/7/08, thisisann@aol.com wrote: > Does anyone know where I can get control tissue.? I know I can purchase slides, but is there a website that I can go to to request control tissue at no cost, tissue such as tonsil, etc. that a hospital is discarding? > Thanks, > Ann > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Mon Jun 9 08:52:35 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jun 9 08:52:47 2008 Subject: [Histonet] Her 2 FISH/PCA3/DASL In-Reply-To: <8CA984608CC4BAA-1160-1630@FWM-D42.sysops.aol.com> Message-ID: <669229.18984.qm@web65712.mail.ac4.yahoo.com> I used to do FISH Her2 (VISSION/Abbot) as part of our histology lab, along with all our IHC, DIF, INDIF procedures. Ren? J. godsgalnow@aol.com wrote: Of the labs of there performing this test, are you doing it as part of your histology lab, or does this go to another department?? What about PCA3?? UroVysion? Anyone doing DASL by Illumina?? Same questions as above, please. Thanks in advance, Roxanne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Mon Jun 9 09:42:54 2008 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Jun 9 09:42:57 2008 Subject: [Histonet] RELIA Histology Spotlight Opportunity re-post with location. The position is located in a Seattle Suburb Message-ID: Hello Histonetters!! How are you? Are you ready to start the summer? I just wanted to make you aware of this opportunity that I feel is a really great one. Just in case you might be interested or know someone who might be interested I am sending this special bulletin out. The reasons that I feel that this is a special opportunity are because 2 candidates placed here by RELIA endorse it as a great place to work and live. Also there is the opportunity to learn new things like electron microscopy. This is the posting that I put on the internet about this position. If you or someone you know might be interested please contact me or have them contact me at relia1@earthlink.net or toll free at 866-607-3542. RELIA the nation's only permanent placement firm dedicated solely to the histology profession is assisting an exciting Research company located in a Seattle suburb with the search for a histology technician or technologist who is ASCP eligible. This client offers excellent salary benefits and relocation assistance. 2 candidates placed here by RELIA endorse it as a great place to work and live. Here is the job description: The Histology Technician/Histotechnologist will be responsible for processing, sectioning, staining, and preparing slides of tissue samples for microscopic evaluation by pathologists. Responsibilities include: Maintain Good Laboratory Practices (GLP). Process, embed, section, and perform necessary stains to highlight specific tissue patterns and structures. Perform quality control (QC) on finished slide preparations. Maintain and troubleshoot equipment operation failures; take appropriate actions to keep interruptions in work flow to a minimum. Execute special histological and histochemical stains used to emphasize specific tissue elements. Perform special histological procedures such as frozen sectioning and electron microscopy preparations (e.g. plastic processing, embedding, sectioning), and photomicrography. Requirements include 2 years current experience in histology or an Associate's (AA) degree or higher, specializing in anatomy-related medical science, plus at least 1 year related experience and/or training. ASCP HT/HTL or eligible. For more information contact Pam Barker at RELIA Solutions. e-mail: relia1@earthlink.net or toll free at 866-60-RELIA (866-607-3542) RELIA Solutions is the only nationwide recruiting firm specializing in the permanent placement of histology professionals Thanks for taking the time to take a look!! Have a great day!! Thank You! Pam M. Barker President Relia 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll Free: (866)607-3542 e-mail: relia1@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia From pumla.pamla <@t> gmail.com Mon Jun 9 09:43:14 2008 From: pumla.pamla <@t> gmail.com (Pumla Pamla-Gutter) Date: Mon Jun 9 09:43:19 2008 Subject: [Histonet] MOR receptors. Message-ID: Is anyone out there working with MOR receptors and having good results in Immunohistochemistry with the MOR antibodies? Thanks, Pumla Pamla-Gutter Ohio State University College of Dentistry Research Assistant I From buckeyedermatology <@t> yahoo.com Mon Jun 9 09:47:11 2008 From: buckeyedermatology <@t> yahoo.com (C. Rodriguez) Date: Mon Jun 9 09:47:14 2008 Subject: [Histonet] Fw: job opportunity Message-ID: <282078.15869.qm@web33206.mail.mud.yahoo.com> --- On Wed, 5/9/07, J. Cruz <buckeyedermatology@yahoo.com> wrote: From: J. Cruz <buckeyedermatology@yahoo.com> Subject: job opportunity To: histonet@lists.utsouthwestern.edu Date: Wednesday, May 9, 2007, 2:53 PM My name is Andrew I work with Buckeye Dermatology her in Columbus ,Oh and we are looking for day shift histologist. You can e-mail your resume to Buckeye Dermatology @ yahoo. com 8:00? 8:25? 8:40? Find a flick in no time with theYahoo! Search movie showtime shortcut. From hej01 <@t> health.state.ny.us Mon Jun 9 09:49:35 2008 From: hej01 <@t> health.state.ny.us (Helen E Johnson) Date: Mon Jun 9 09:49:43 2008 Subject: [Histonet] frozen sections Message-ID: I have some frozen, unfixed tissues that I now wish I had fixed so that I could section them. How difficult / possible would it be to cut and fix sections from these tissues that would be suitable for immunohistochemistry or fluorescence? Helen Johnson (hej01@health.state.ny.us) IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. From buckeyedermatology <@t> yahoo.com Mon Jun 9 10:00:11 2008 From: buckeyedermatology <@t> yahoo.com (C. Rodriguez) Date: Mon Jun 9 10:00:17 2008 Subject: [Histonet] Fw: job opportunity Message-ID: <430988.667.qm@web33207.mail.mud.yahoo.com> --- On Wed, 5/9/07, J. Cruz <buckeyedermatology@yahoo.com> wrote: From: J. Cruz <buckeyedermatology@yahoo.com> Subject: job opportunity To: histonet@lists.utsouthwestern.edu Date: Wednesday, May 9, 2007, 3:55 PM My name is Andrew I work with Buckeye Dermatology her in Columbus ,Oh and we are looking for day shift histologist. You can e-mail your resume to Buckeye Dermatology @ yahoo. com Expecting? Get great news right away with email Auto-Check. Try the Yahoo! Mail Beta. From buckeyedermatology <@t> yahoo.com Mon Jun 9 10:00:05 2008 From: buckeyedermatology <@t> yahoo.com (C. Rodriguez) Date: Mon Jun 9 10:00:18 2008 Subject: [Histonet] Fw: job opportunity Message-ID: <188417.27148.qm@web33206.mail.mud.yahoo.com> --- On Wed, 5/9/07, J. Cruz <buckeyedermatology@yahoo.com> wrote: From: J. Cruz <buckeyedermatology@yahoo.com> Subject: job opportunity To: histonet@lists.utsouthwestern.edu Date: Wednesday, May 9, 2007, 3:55 PM My name is Andrew I work with Buckeye Dermatology her in Columbus ,Oh and we are looking for day shift histologist. You can e-mail your resume to Buckeye Dermatology @ yahoo. com Expecting? Get great news right away with email Auto-Check. Try the Yahoo! Mail Beta. From valeria.berno <@t> embl.it Mon Jun 9 07:31:55 2008 From: valeria.berno <@t> embl.it (Valeria Berno) Date: Mon Jun 9 10:19:30 2008 Subject: [Histonet] pAKT In-Reply-To: <1164.74.127.102.244.1212801668.squirrel@webmail.ihctech.net> References: <1164.74.127.102.244.1212801668.squirrel@webmail.ihctech.net> Message-ID: <1220.10.251.1.111.1213014715.squirrel@10.251.1.111> Hi sorry if I jump in the discussion but I am just starting to test the pAKT from cell signalling (ser473) on brain cryosection. could you please gave me some advise on the protocol you were using? blocking with BSA,milk or serum? did you use 1:25 dilution as they suggest? triton 0.3% in blocking step and in primary antibody incubation?? some peculiar advise? thanks Valeria Berno Valeria, PhD EMBL- Mouse Biology Unit Campus A. Buzzati-Traverso Via Ramarini, 32 00015, Monterotondo Scalo (RM) Italy Tel: +39 06 90091243 Fax: +39 06 90091406 email: valeria.berno@embl.it www.embl.it > cell signaling has a couple of alternative abs for pAKT they claim are > better, i believe they are rab monoclonals, i have not tried them yet. > > Patsy > > >> What Abs are people using for pAKT these days? The one I had working >> so well in FFPE is no longer being made by Cell Signaling. >> >> Thanks, >> Andrea >> -- >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Berno Valeria, PhD EMBL- Mouse Biology Unit Campus A. Buzzati-Traverso Via Ramarini, 32 00015, Monterotondo Scalo (RM) Italy Tel: +39 06 90091243 Fax: +39 06 90091406 email: valeria.berno@embl.it www.embl.it From jeff.oliver <@t> asterand.com Mon Jun 9 10:48:00 2008 From: jeff.oliver <@t> asterand.com (Jeff Oliver) Date: Mon Jun 9 10:48:07 2008 Subject: [Histonet] Dako Autostainer In-Reply-To: References: Message-ID: Hi Gabriela, That sound familiar! Replacing the probe would sometimes help for a few days, but after that our autostainer would stop detecting reagents again. After several visits DAKO replaced the entire unit. It was a "new" reconditioned one, but no problems since. Pure speculation, but I would bet on a bit of corrosion or a bad capacitor as the problem. -Jeff -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DeMatteo, Gabriela Sent: Friday, June 06, 2008 11:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dako Autostainer About the same problem, beside the sensor malfunction, could the software be the problem? Our "immuno specialist" said that the service tech found nothing wrong with the Dako instrument. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Jun 9 11:22:48 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jun 9 11:22:56 2008 Subject: [Histonet] frozen sections Message-ID: <761329.96019.qm@web65708.mail.ac4.yahoo.com> If they have been frozen embedded in OCT at low temp. (-80?C), you can go ahead and cut them and do your tests. If you want to fix them to prepare FFPE blocks, thaw and fix them and porcede as if they were "fresh" tissue. Ren? J. Helen E Johnson wrote: I have some frozen, unfixed tissues that I now wish I had fixed so that I could section them. How difficult / possible would it be to cut and fix sections from these tissues that would be suitable for immunohistochemistry or fluorescence? Helen Johnson (hej01@health.state.ny.us) IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kappeler <@t> patho.unibe.ch Mon Jun 9 11:41:11 2008 From: kappeler <@t> patho.unibe.ch (Andi Kappeler) Date: Mon Jun 9 11:41:30 2008 Subject: [Histonet] IHC for estrogen receptor beta Message-ID: <002501c8ca4f$a0342c20$17955c82@pi23> Hi all has anybody been successfully using mouse-anti hu estrogen receptor beta, clone 14C8 (GeneTex) on FFPE tissue? We have tried 8 different pretreatment variants on 6 different samples of breast carcinoma (including normal mammary glands) at Ig conc of 4 and 8 mg/l. Visualization with polymer/HRP and DAB. If you were successful, would you mind sharing your protocol? If you were successful with a different anti-estrogen receptor beta antibody, I would be interested, too (clone designation, company)! Many thanks! Andi Kappeler Institute of Pathology, University of Bern, Switzerland From alexandra.meinl <@t> gmail.com Mon Jun 9 11:58:32 2008 From: alexandra.meinl <@t> gmail.com (Alexandra Meinl) Date: Mon Jun 9 11:58:38 2008 Subject: [Histonet] stain for fibrin clots/adhaesives Message-ID: dear all, i'm trying to find a sufficiently working stain for fibrinclots and fibrin adhaesives. i've some frustrating experience with ladewig's trichrome and the msb-technique, both didn't stain this type of fibrin well. in both cases most of the fibrin remained blue (like collagen), even a pure fibrinclot remained negative for fibrin. does anybody have experience with this type of sample? thanks! Alexandra From Linda.Watson <@t> bms.com Mon Jun 9 11:59:19 2008 From: Linda.Watson <@t> bms.com (Linda M Watson) Date: Mon Jun 9 11:59:22 2008 Subject: [Histonet] frozen sections In-Reply-To: <200806091450.m59EoZ95029244@meusplapp03.net.bms.com> References: <200806091450.m59EoZ95029244@meusplapp03.net.bms.com> Message-ID: <484D6167.5000307@bms.com> If they are not embedded in OCT then place them in 10% NBF-fix for appropriate amount of time prior to processing.You may have some freezer burn around the edges even after fixation but the rest of the tissue should be OK. If they are embedded in OCT you can either thaw it out remove the tissue as best as you can from the OCT and place the sample in 10% NBF(fix and process appropriately). If you want to section the frozen first and follow with formalin fixation prior to the IHC that sometimes works and gives better morphology than acetone fixation. Good luck, Linda Helen E Johnson wrote: >I have some frozen, unfixed tissues that I now wish I had fixed so that I >could section them. How difficult / possible would it be to cut and fix >sections from these tissues that would be suitable for immunohistochemistry >or fluorescence? >Helen Johnson (hej01@health.state.ny.us) > > >IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From luke.perkocha <@t> ucsf.edu Mon Jun 9 12:00:04 2008 From: luke.perkocha <@t> ucsf.edu (Perkocha, Luke) Date: Mon Jun 9 12:01:31 2008 Subject: [Histonet] Histology robotics and combined stainer - coverslipper products Message-ID: Hi All, We are planning a new histology lab and I remember hearing about one (or more?) of the vendors that either has or more likely is working on an integrated stainer / coverslipper product, that can be put together in sort of a robotic production line. I'm not sure if they're there yet, but is anyone aware of who's working on this or what is available now? We'd like to find out what's planned so that as we design spaces, it is done in a way that accommodates this type of thing. Many thanks, Luke Perkocha From hborgeri <@t> wfubmc.edu Mon Jun 9 12:04:02 2008 From: hborgeri <@t> wfubmc.edu (Hermina Borgerink) Date: Mon Jun 9 12:04:06 2008 Subject: [Histonet] IHC for estrogen receptor beta In-Reply-To: <002501c8ca4f$a0342c20$17955c82@pi23> References: <002501c8ca4f$a0342c20$17955c82@pi23> Message-ID: <9AEEF1FB6254224AA355ED285F8491652CD7B123@EXCHVS2.medctr.ad.wfubmc.edu> Andi, I have succesfully used Novocastra's mouse monoclonal ER beta (Clone EMR02) on both human and non-human primates. Pre-treatment with Citrate buffer at pH 6.0 for 30 minutes using a Black and Decker rice steamer, with a 20-minute cooling down period. I dilute my antibody at 1:50 in Tris buffer containing 0.5% casein and Tween-20, and incubate overnight at 4 degrees C. I use an anti-mouse biotinylated secondary (1:20) and a streptavidin-alkalinephosphatase (1:20) from BioGenex,both for 20 minutes at 37 degrees C. I use Vector Red substrate for visualization, and counterstain with Mayer's hematoxylin. Hermina -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andi Kappeler Sent: Monday, June 09, 2008 12:41 PM To: Histonet Subject: [Histonet] IHC for estrogen receptor beta Hi all has anybody been successfully using mouse-anti hu estrogen receptor beta, clone 14C8 (GeneTex) on FFPE tissue? We have tried 8 different pretreatment variants on 6 different samples of breast carcinoma (including normal mammary glands) at Ig conc of 4 and 8 mg/l. Visualization with polymer/HRP and DAB. If you were successful, would you mind sharing your protocol? If you were successful with a different anti-estrogen receptor beta antibody, I would be interested, too (clone designation, company)! Many thanks! Andi Kappeler Institute of Pathology, University of Bern, Switzerland _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AFoshey <@t> chw.org Mon Jun 9 12:36:05 2008 From: AFoshey <@t> chw.org (Foshey, Annette) Date: Mon Jun 9 12:36:11 2008 Subject: [Histonet] Processing Brain specimens - pediactric autopsy Message-ID: <9E6D52F532809247BDA1783680E92C561B4436F4@CHWEXC.chwi.chswi.org> Does anyone have a protocol for processing brain on a VIP 5 or comparable processor for Brain tissues in cassettes? I would appreciate any input. Thanks, Annette Foshey, HT (ASCP) Team Leader in Histology Children's Hospital of Wisconsin 414-266-6580 Fax 414-266-2779 afoshey@chw.org ************************** Children's Hospital and Health System recognizes that unencrypted e-mail is not secure and does not guarantee confidentiality. The confidentiality of replies to this message cannot be guaranteed unless the replies are encrypted. From alexandra.meinl <@t> gmail.com Mon Jun 9 12:45:35 2008 From: alexandra.meinl <@t> gmail.com (Alexandra Meinl) Date: Mon Jun 9 12:45:38 2008 Subject: [Histonet] stain for fibrin clots/adhaesives In-Reply-To: References: Message-ID: dear all, i'm trying to find a sufficiently working stain for fibrinclots and fibrin adhaesives. i've some frustrating experience with ladewig's trichrome and the msb-technique, both didn't stain this type of fibrin well. in both cases most of the fibrin remained blue (like collagen), even a pure fibrinclot remained negative for fibrin. does anybody have experience with this type of sample? thanks! Alexandra From renafail <@t> bellsouth.net Mon Jun 9 13:00:03 2008 From: renafail <@t> bellsouth.net (Rena Fail) Date: Mon Jun 9 13:00:06 2008 Subject: [Histonet] stain for fibrin clots/adhaesives In-Reply-To: Message-ID: <000601c8e2b6$8cca1bc0$0301a8c0@RENAD4YK9B8ABE> Try the Fraser-Lendrum stain for fibrin in the AFIP manual. It's easy and the results are nice Rena Fail -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alexandra Meinl Sent: Monday, June 09, 2008 1:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] stain for fibrin clots/adhaesives dear all, i'm trying to find a sufficiently working stain for fibrinclots and fibrin adhaesives. i've some frustrating experience with ladewig's trichrome and the msb-technique, both didn't stain this type of fibrin well. in both cases most of the fibrin remained blue (like collagen), even a pure fibrinclot remained negative for fibrin. does anybody have experience with this type of sample? thanks! Alexandra _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ploykasek <@t> phenopath.com Mon Jun 9 13:34:41 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Mon Jun 9 13:34:51 2008 Subject: [Histonet] VZV control Message-ID: Hi all. Does anyone know of a source for positive control material (paraffin blocks or cut sections) for varicella zoster virus? I have been unable to find a commercial source. Thank you. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From Terri.Brown <@t> Northside.com Mon Jun 9 14:03:52 2008 From: Terri.Brown <@t> Northside.com (Terri Brown) Date: Mon Jun 9 14:04:14 2008 Subject: [Histonet] NSH Histology Task Analysis and StandardsofPerformance Competen cy In-Reply-To: <30657.26774.qm@web36503.mail.mud.yahoo.com> References: <30657.26774.qm@web36503.mail.mud.yahoo.com> Message-ID: <8CEB6DA1A3F35743800669D4CFE21F7D03F90086@NSMXMS04.northside.local> I would like a copy too. Terri H. Brown Pathology Laboratory Manager Northside Hospital Office: 404-845-5423 Fax: 404-851-6400 terri.brown@northside.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Sent: Saturday, June 07, 2008 12:53 PM To: Norm Burnham; Rathborne, Toni; Mahoney,Janice A; McKnight, Tanisha; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] NSH Histology Task Analysis and StandardsofPerformance Competen cy I would be most greatfull for a copy too. Linda Dee Chicago, IL ----- Original Message ---- From: Norm Burnham To: "Rathborne, Toni" ; "Mahoney,Janice A" ; "McKnight, Tanisha" ; histonet@lists.utsouthwestern.edu Sent: Friday, June 6, 2008 10:48:33 AM Subject: RE: [Histonet] NSH Histology Task Analysis and StandardsofPerformance Competen cy Me too!? Norm Burnham -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, June 06, 2008 10:27 AM To: Mahoney,Janice A; McKnight, Tanisha; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH Histology Task Analysis and StandardsofPerformance Competen cy As would I. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Mahoney,Janice A Sent: Friday, June 06, 2008 11:23 AM To: 'McKnight, Tanisha'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH Histology Task Analysis and Standards ofPerformance Competen cy I'd love to have an electronic copy as well Jan Mahoney Alegent Health Omaha,NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McKnight, Tanisha Sent: Friday, June 06, 2008 10:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NSH Histology Task Analysis and Standards of Performance Competen cy Hello Histo-netters: Would anyone happen to have an electronic copy of NSH Histology Task Analysis and The Standards of Performance/Competency that you could send to me? I am in desperate need of these items. I've contacted NSH for these twice, but I have gotten no response. I'd greatly appreciate anything you could send. Tanisha N. McKnight, HT (ASCP) Covance CLS Indianapolis Specimen Management, Anatomic Pathology ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful.? If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer.? Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening.? Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail.? Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law.? Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.? If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. ______________________________________________________________________________ This e-mail may contain confidential or privileged information. If you think you have received this e-mail in error, please advise the sender by reply e-mail and then delete this e-mail immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. From MLashus <@t> pathgroup.com Mon Jun 9 14:29:46 2008 From: MLashus <@t> pathgroup.com (Mighnon Lashus) Date: Mon Jun 9 14:29:51 2008 Subject: [Histonet] NSH Histology Task Analysis and StandardsofPerformance Competen cy In-Reply-To: <8CEB6DA1A3F35743800669D4CFE21F7D03F90086@NSMXMS04.northside.local> Message-ID: <197CD0B02A81F94994A285C59C8AE05C03386C8318@pgnexchange.pathgroup.com> I just got back from the Bahamas, but I would love to have a copy. Thanks, Mighnon Lashus Chattanooga, TN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Brown Sent: Monday, June 09, 2008 3:04 PM To: Linda; Norm Burnham; Rathborne, Toni; Mahoney,Janice A; McKnight, Tanisha; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH Histology Task Analysis and StandardsofPerformance Competen cy I would like a copy too. Terri H. Brown Pathology Laboratory Manager Northside Hospital Office: 404-845-5423 Fax: 404-851-6400 terri.brown@northside.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Sent: Saturday, June 07, 2008 12:53 PM To: Norm Burnham; Rathborne, Toni; Mahoney,Janice A; McKnight, Tanisha; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] NSH Histology Task Analysis and StandardsofPerformance Competen cy I would be most greatfull for a copy too. Linda Dee Chicago, IL ----- Original Message ---- From: Norm Burnham To: "Rathborne, Toni" ; "Mahoney,Janice A" ; "McKnight, Tanisha" ; histonet@lists.utsouthwestern.edu Sent: Friday, June 6, 2008 10:48:33 AM Subject: RE: [Histonet] NSH Histology Task Analysis and StandardsofPerformance Competen cy Me too! Norm Burnham -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, June 06, 2008 10:27 AM To: Mahoney,Janice A; McKnight, Tanisha; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH Histology Task Analysis and StandardsofPerformance Competen cy As would I. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Mahoney,Janice A Sent: Friday, June 06, 2008 11:23 AM To: 'McKnight, Tanisha'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH Histology Task Analysis and Standards ofPerformance Competen cy I'd love to have an electronic copy as well Jan Mahoney Alegent Health Omaha,NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McKnight, Tanisha Sent: Friday, June 06, 2008 10:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NSH Histology Task Analysis and Standards of Performance Competen cy Hello Histo-netters: Would anyone happen to have an electronic copy of NSH Histology Task Analysis and The Standards of Performance/Competency that you could send to me? I am in desperate need of these items. I've contacted NSH for these twice, but I have gotten no response. I'd greatly appreciate anything you could send. Tanisha N. McKnight, HT (ASCP) Covance CLS Indianapolis Specimen Management, Anatomic Pathology ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. ______________________________________________________________________________ This e-mail may contain confidential or privileged information. If you think you have received this e-mail in error, please advise the sender by reply e-mail and then delete this e-mail immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From Jackie.O'Connor <@t> abbott.com Mon Jun 9 15:02:22 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Mon Jun 9 15:02:37 2008 Subject: [Histonet] Histology job fair items In-Reply-To: <188417.27148.qm@web33206.mail.mud.yahoo.com> Message-ID: Where can I find histology literature to try to scam some kids into getting into the field? I mean - encourage some young minds to consider histology. I checked the NSH website, thinking I'd ordered some in the past - but didn't find any info. Anyone have any ideas? I need brochures or handouts of somekind. Willing to pay $$. Jackie O' From godsgalnow <@t> aol.com Mon Jun 9 15:17:02 2008 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Mon Jun 9 15:17:18 2008 Subject: [Histonet] NSH Histology Task Analysis and StandardsofPerformance Competen cy In-Reply-To: <197CD0B02A81F94994A285C59C8AE05C03386C8318@pgnexchange.pathgroup.com> Message-ID: <8CA9884E9934429-B6C-7A6@webmail-dd20.sysops.aol.com> You can all get a copy by emailing NSH and asking for one Roxanne -----Original Message----- From: Mighnon Lashus To: histonet@lists.utsouthwestern.edu Sent: Mon, 9 Jun 2008 3:29 pm Subject: RE: [Histonet] NSH Histology Task Analysis and StandardsofPerformance Competen cy I just got back from the Bahamas, but I would love to have a copy. Thanks, Mighnon Lashus Chattanooga, TN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Brown Sent: Monday, June 09, 2008 3:04 PM To: Linda; Norm Burnham; Rathborne, Toni; Mahoney,Janice A; McKnight, Tanisha; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH Histology Task Analysis and StandardsofPerformance Competen cy I would like a copy too. Terri H. Brown Pathology Laboratory Manager Northside Hospital Office: 404-845-5423 Fax: 404-851-6400 terri.brown@northside.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Sent: Saturday, June 07, 2008 12:53 PM To: Norm Burnham; Rathborne, Toni; Mahoney,Janice A; McKnight, Tanisha; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] NSH Histology Task Analysis and StandardsofPerformance Competen cy I would be most greatfull for a copy too. Linda Dee Chicago, IL ----- Original Message ---- From: Norm Burnham To: "Rathborne, Toni" ; "Mahoney,Janice A" ; "McKnight, Tanisha" ; histonet@lists.utsouthwestern.edu Sent: Friday, June 6, 2008 10:48:33 AM Subject: RE: [Histonet] NSH Histology Task Analysis and StandardsofPerformance Competen cy Me too! Norm Burnham -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, June 06, 2008 10:27 AM To: Mahoney,Janice A; McKnight, Tanisha; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH Histology Task Analysis and StandardsofPerformance Competen cy As would I. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Mahoney,Janice A Sent: Friday, June 06, 2008 11:23 AM To: 'McKnight, Tanisha'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH Histology Task Analysis and Standards ofPerformance Competen cy I'd love to have an electronic copy as well Jan Mahoney Alegent Health Omaha,NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McKnight, Tanisha Sent: Friday, June 06, 2008 10:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NSH Histology Task Analysis and Standards of Performance Competen cy Hello Histo-netters: Would anyone happen to have an electronic copy of NSH Histology Task Analysis and The Standards of Performance/Competency that you could send to me? I am in desperate need of these items. I've contacted NSH for these twice, but I have gotten no response. I'd greatly appreciate anything you could send. Tanisha N. McKnight, HT (ASCP) Covance CLS Indianapolis Specimen Management, Anatomic Pathology ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. ______________________________________________________________________________ This e-mail may contain confidential or privileged information. If you think you have received this e-mail in error, please advise the sender by reply e-mail and then delete this e-mail immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Jun 9 15:21:47 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jun 9 15:21:51 2008 Subject: [Histonet] Histology robotics and combined stainer - coverslipper products In-Reply-To: Message-ID: <961475.27633.qm@web65703.mail.ac4.yahoo.com> Both Sakura and Leica have such a setting. Ren? J. "Perkocha, Luke" wrote: Hi All, We are planning a new histology lab and I remember hearing about one (or more?) of the vendors that either has or more likely is working on an integrated stainer / coverslipper product, that can be put together in sort of a robotic production line. I'm not sure if they're there yet, but is anyone aware of who's working on this or what is available now? We'd like to find out what's planned so that as we design spaces, it is done in a way that accommodates this type of thing. Many thanks, Luke Perkocha _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgalnow <@t> aol.com Mon Jun 9 15:34:32 2008 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Mon Jun 9 15:34:45 2008 Subject: [Histonet] Histology job fair items In-Reply-To: Message-ID: <8CA98875B96CD09-B6C-8E4@webmail-dd20.sysops.aol.com> I just had a huge career day week here during lab week.? We targeted 8 th graders from the area.? I contacted all of the area schools (the career counselors) to get them interested and here.? Once that was done, I contacted NSH...(Brenda is great or Pebbles, they will be happy to assist you) and had them send me 400 career brochures.? I went to the candy warehouse online and ordered all kinds of body part candy.? I went to the party store and bought bags to make goody bags, I also bought stuff to make banners (one for each school).? I contacted my vendors and asked for donations and BioCare was awesome...they sent me 400 kidney stress balls, tattoos, pens, magnets...you name it.? I spent hours and hours putting goody bags together.? I set up multiple stations here and had the kids for 2 hours and toured them around. It was awesome. We got many letters from the schools and students. Roxanne -----Original Message----- From: Jackie M O'Connor To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Sent: Mon, 9 Jun 2008 4:02 pm Subject: [Histonet] Histology job fair items Where can I find histology literature to try to scam some kids into getting into the field? I mean - encourage some young minds to consider histology. I checked the NSH website, thinking I'd ordered some in the past - but didn't find any info. Anyone have any ideas? I need brochures or handouts of somekind. Willing to pay $$. Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jennifer.bull <@t> northwestpathology.com Mon Jun 9 15:37:11 2008 From: jennifer.bull <@t> northwestpathology.com (Bull, Jennifer L.) Date: Mon Jun 9 15:37:17 2008 Subject: [Histonet] Job Opportunity in Washington State Message-ID: <719B46988560834BBE7D7F72EEB7C10A01853553@HINET1.hinet.org> Histology Technition- Northwest Pathology, a high volume, fast paced, growing, private laboratory located near the ocean and the mountains, between Seattle and Vancouver, British Columbia in Bellingham, WA. seeks a full-time, Histology Technician, ASCP certified or registry eligible preferred. Responsibilities include a variety of routine and specialized laboratory tests and procedures, instrument maintenance, quality control, assisting in employee training, ability and willingness to learn new tests, in a highly specialized laboratory environment. NWP offers comprehensive benefit package, candidate must be willing to work various shifts as needed, multitask, problem solve, be flexible in perspective, attentive to detail, meet varying daily work requirements that include specific and volume based deadlines with an understanding of the importance of team building. For information contact Jennifer Bull at jennifer.bull@nwpathology.com From dellav <@t> musc.edu Mon Jun 9 16:40:29 2008 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Mon Jun 9 16:38:41 2008 Subject: [Histonet] NSH Histology Task Analysis and Standards of Performance Competen cy In-Reply-To: <816E3C72F855F14985FC31D7C963AE6F0762E989@indexch03.ent.covance.com> References: <816E3C72F855F14985FC31D7C963AE6F0762E989@indexch03.ent.covance.com> Message-ID: In reviewing the messages of this thread I came across Tanisha's message. I'd like to reiterate that the Task Analysis is available only to NSH members. Tanisha's message gives the impression that NSH has been unresponsive to her. I am writing to clear the record and to assist her. Tanisha is not listed as a current member according to the NSH member directory. If this information is incorrect Tanisha, please contact the NSH office to have these records corrected. If you are unsuccessful in getting this issue resolved, I invite you to phone me directly and I will be happy to intercede on your behalf. I've listed my phone number in my signature at the bottom of these remarks. For those of you who might find the Task Analysis of value but are not members, I would encourage you to consider affiliating with the society. Dues are very affordable ($60) and there are many resources available to members that you will find desirable and helpful to you in your work. We have many programs and are working diligently on behalf of NSH members throughout the year. We are happy to assist you in meeting your professional goals. Vinnie Della Speranza President, National Society for Histotechnology (843) 792-6353 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McKnight, Tanisha Sent: Friday, June 06, 2008 11:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NSH Histology Task Analysis and Standards of Performance Competen cy Hello Histo-netters: Would anyone happen to have an electronic copy of NSH Histology Task Analysis and The Standards of Performance/Competency that you could send to me? I am in desperate need of these items. I've contacted NSH for these twice, but I have gotten no response. I'd greatly appreciate anything you could send. Tanisha N. McKnight, HT (ASCP) Covance CLS Indianapolis Specimen Management, Anatomic Pathology ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From judi.ford <@t> roche.com Mon Jun 9 16:53:48 2008 From: judi.ford <@t> roche.com (Ford, Judi) Date: Mon Jun 9 16:54:00 2008 Subject: [Histonet] Tissue Capture Pen Message-ID: Hi everyone out there....... We've just been given a "Tissue Capture" pen to use as an adhesive for glass slides. These slides are to be used in IHC. Has anyone ever used this pen? If so, what type of slides do you use (plain glass vs "+" slides?). If you use it on "+" slides, why? Doesn't it cover up the adhesiveness (sp) of the "+" slides, or does it add extra adhesion? For paraffin do you use room temp water in your waterbath, or warm water? I really appreciate any helpful hints. Thanks soooo much! Judi Ford Research Associate Roche Palo Alto, CA From tanisha.mcknight <@t> covance.com Mon Jun 9 19:35:53 2008 From: tanisha.mcknight <@t> covance.com (McKnight, Tanisha) Date: Mon Jun 9 19:36:06 2008 Subject: [Histonet] NSH Histology Task Analysis and Standards of Perfo rmance Competen cy In-Reply-To: <816E3C72F855F14985FC31D7C963AE6F0762E989@indexch03.ent.covance.com> References: <816E3C72F855F14985FC31D7C963AE6F0762E989@indexch03.ent.covance.com> Message-ID: <816E3C72F855F14985FC31D7C963AE6F0491B6AE@indexch03.ent.covance.com> Hello Vinnie: Thank you for responding to this matter. I'd like to assure you that I am indeed a member of NSH. You may want to conduct your search under my unmarried name Tanisha Neely. It was not my intent to cause a problem or imply something negative about NSH. I love the work that you do and I would encourage anyone in our field to become a member. However, I have sent 2 separate requests for these items by email and made one request by phone (I was told the person who handled this wasn't in). I have yet to receive a response. Thank you again for yours. Sincerely, Tanisha Neely McKnight -----Original Message----- From: Della Speranza, Vinnie To: McKnight, Tanisha; histonet@lists.utsouthwestern.edu Sent: 6/9/2008 5:40 PM Subject: RE: [Histonet] NSH Histology Task Analysis and Standards of Performance Competen cy In reviewing the messages of this thread I came across Tanisha's message. I'd like to reiterate that the Task Analysis is available only to NSH members. Tanisha's message gives the impression that NSH has been unresponsive to her. I am writing to clear the record and to assist her. Tanisha is not listed as a current member according to the NSH member directory. If this information is incorrect Tanisha, please contact the NSH office to have these records corrected. If you are unsuccessful in getting this issue resolved, I invite you to phone me directly and I will be happy to intercede on your behalf. I've listed my phone number in my signature at the bottom of these remarks. For those of you who might find the Task Analysis of value but are not members, I would encourage you to consider affiliating with the society. Dues are very affordable ($60) and there are many resources available to members that you will find desirable and helpful to you in your work. We have many programs and are working diligently on behalf of NSH members throughout the year. We are happy to assist you in meeting your professional goals. Vinnie Della Speranza President, National Society for Histotechnology (843) 792-6353 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McKnight, Tanisha Sent: Friday, June 06, 2008 11:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NSH Histology Task Analysis and Standards of Performance Competen cy Hello Histo-netters: Would anyone happen to have an electronic copy of NSH Histology Task Analysis and The Standards of Performance/Competency that you could send to me? I am in desperate need of these items. I've contacted NSH for these twice, but I have gotten no response. I'd greatly appreciate anything you could send. Tanisha N. McKnight, HT (ASCP) Covance CLS Indianapolis Specimen Management, Anatomic Pathology ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From b-frederick <@t> northwestern.edu Tue Jun 10 08:20:30 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue Jun 10 08:20:44 2008 Subject: [Histonet] stain for fibrin clots/adhaesives In-Reply-To: <000601c8e2b6$8cca1bc0$0301a8c0@RENAD4YK9B8ABE> Message-ID: <000001c8cafc$c475c140$d00f7ca5@lurie.northwestern.edu> I believe Rowley biochemical sells the reagents for it as well as Ayoub-shklar. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rena Fail Sent: Thursday, July 10, 2008 12:58 PM To: 'Alexandra Meinl'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] stain for fibrin clots/adhaesives Try the Fraser-Lendrum stain for fibrin in the AFIP manual. It's easy and the results are nice Rena Fail -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alexandra Meinl Sent: Monday, June 09, 2008 1:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] stain for fibrin clots/adhaesives dear all, i'm trying to find a sufficiently working stain for fibrinclots and fibrin adhaesives. i've some frustrating experience with ladewig's trichrome and the msb-technique, both didn't stain this type of fibrin well. in both cases most of the fibrin remained blue (like collagen), even a pure fibrinclot remained negative for fibrin. does anybody have experience with this type of sample? thanks! Alexandra _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jhubbart <@t> mcrmc.org Tue Jun 10 09:01:06 2008 From: jhubbart <@t> mcrmc.org (Hubbarth-Lepera, Jennifer) Date: Tue Jun 10 09:01:55 2008 Subject: [Histonet] RE: Histonet Digest, Vol 55, Issue 13 Message-ID: I wanted to say something about the negative controls "The positive control slide or patient test slides, if these slides contain tissue elements that should not react with the antibody." This means to me and my facility that there is no need for negative controls- that your negative is making sure your positive control is staining correctly and not staining what it isn't supposed to and you have an accurate control. Jen MCRMC Mi -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Saturday, June 07, 2008 12:55 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 55, Issue 13 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Looking for Work (Steven Coakley) 2. RE: Sally's Friday Fume (Joyce Cline) 3. Veterinary Histologist Certification Eligibility (Breeden, Sara) 4. Re: Sally's Friday Fume (Shelly Christenson) 5. Job Opening Columbus, OH (Gullifer, Dawn) 6. RE: Sally's Friday Fume (Gessford, Mary) 7. unsubscribe (Terry Costello) 8. Peroxidase blocking in frozen sections (JMyers1@aol.com) 9. RE: Sally's Friday Fume (Liz Chlipala) 10. RE: Veterinary Histologist Certification Eligibility (Della Speranza, Vinnie) 11. SLide Holder (Behnaz Sohrab) 12. IHC neg. controls (Friday rant) (Patti Loykasek) 13. Re: IHC neg. controls (Friday rant) (Patti Loykasek) 14. Re: IHC neg. controls (Friday rant) (Larry Woody) 15. Re: IHC neg. controls (Friday rant) (Rene J Buesa) 16. RE:Peroxidase blocking in frozen sections (JR R) 17. Re: IHC neg. controls (Friday rant) (pruegg@ihctech.net) 18. pAKT (Andrea Hooper) 19. Re: pAKT (pruegg@ihctech.net) 20. Re: Sally's Friday Fume (Janet Maass) 21. Disposing of used paraffin from the tissue processor (Scott, Allison D) 22. Re: Disposing of used paraffin from the tissue processor (Rene J Buesa) 23. Re: NSH Histology Task Analysis and StandardsofPerformance Competen cy (Linda) ---------------------------------------------------------------------- Message: 1 Date: Fri, 6 Jun 2008 10:31:58 -0700 (PDT) From: Steven Coakley Subject: [Histonet] Looking for Work To: Histonet@lists.utsouthwestern.edu Message-ID: <193638.55208.qm@web38201.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I reside in South Central WI. Looking for work in this area, No. WI, No.Mich. VT, or NH. Thanks everyone and have a "grand" weekend. Steve ------------------------------ Message: 2 Date: Fri, 6 Jun 2008 13:39:12 -0400 From: "Joyce Cline" Subject: [Histonet] RE: Sally's Friday Fume To: "Histonet" Message-ID: <74D382FF830A469BA98DB51E36C9FBF5@wchsys.org> Content-Type: text/plain; charset="US-ASCII" I received my HT while I worked in research, under a veterinarian pathologist. ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ------------------------------ Message: 3 Date: Fri, 6 Jun 2008 12:03:39 -0600 From: "Breeden, Sara" Subject: [Histonet] Veterinary Histologist Certification Eligibility To: Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E6370@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" That's a mouthful! Anyway - thank you to those who responded to me. I searched out a board-certified pathologist (non-veterinary) who works in our building (we share a State of NM building with OMI and the State Laboratory) and he was as astounded as I. I appreciate the feedback about writing a letter to BOR requesting a "waiver" for a veterinary situation and I will try that when the time comes. Our lab, in anticipation of our new building (2010) is operating under conditional AALVD certification but certainly not under CAP/JCHAO. I'm thinking of asking for a definition of "medical scientist" from the ASCP. The saga continues, but "where there's a will, there's a way". One would think anyone who is interested in becoming a certified histologist would receive the maximum amount of encouragement, from the employer AND the certifying agency. Perhaps it's time for a review of the requirements or at least of the definition of an employer's validity to provide appropriate training, hmmm??? Mr. Della Speranza, do you have a take on this? It is not my goal here to fluster, annoy, or pester. It's just that I'm beginning to see a certain amount of reluctance on the part of those institutions whose interest should be to encourage the growth of our profession. Now that my aim is to interest local mid-high and high school forensic science students in a detour to the Paraffin Side, I have a path littered with roadblocks. If our profession had the same PR firm that the anti-tobacco industry had, we'd be up to our earlobes in histotechs (did that come out right?). Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ------------------------------ Message: 4 Date: Fri, 06 Jun 2008 13:14:12 -0500 From: "Shelly Christenson" Subject: Re: [Histonet] Sally's Friday Fume To: Message-ID: <48493823.EF61.003F.0@vet.k-state.edu> Content-Type: text/plain; charset=US-ASCII I work in a veterinary diagnostic lab and got my HT certification in 1993 without any problems and they accepted our veterinary pathologist signature. Two other techs got certified later and no problems there either. Shelly Christenson HT(ASCP) Kansas State University Veterinary Diagnostic Lab Manhattan, Kansas 66506 >>> "Breeden, Sara" 6/6/2008 11:28 AM >>> Okay, I finally have something to Fume about. I am beginning to train a person to become a certified histologist. She is a certified veterinary tech with an Associates Degree and the appropriate number of chemistry/biology hours. I inquired of ASCP about her eligibility (after we finish the training period) to take the HT exam. According to ASCP, as a vet diagnostic lab, we do not meet the ASCP requirements for eligibility because we are not accredited by CAP, JCAHO/AABB and do not have a board-certified (read "human") pathologist/medical scientist (hmmm? Could one of our veterinary pathologists fulfill the description of "medical scientist"??). Surely there are other persons working in a veterinary pathology lab that would be interested in certification, one would think. What are they to do? This frosts my petunias and I'd like to know if anyone has any experience or input on this. But - hey! - some of my fluids/solutions in gallon/liter containers seem to have been improved so they don't drip, so maybe someone out there IS listening (that was my first Friday Hour of Fuming subject). Hasta lumbago. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Fri, 6 Jun 2008 14:31:31 -0400 From: "Gullifer, Dawn" Subject: [Histonet] Job Opening Columbus, OH To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=iso-8859-1 All Interested- We have a job opening for a Anatomic Pathology Technologist at OSU Histology Lab, LLC. We are located in Columbus Ohio and are affiliated with The Ohio State University Medical Center. The duties include accessioning, gross examination of biopsy specimens including dictation, preparing tissue for processing and all other normal laboratory duties (inventory, recording, supplies etc....). Experience Requirements are a 2 yr. associates degree in science related field or minimum 24 semester hours (36 quarter hours) of biology, chemistry, physics, and math. The Job Qualifications include training or experience as a tissue prosector desired; certification or experience as a histology technologist desired; and certification, training or experience as an anatomic pathology technician desired. For additional information and for qualifying candidates, please contact me at dawn.gullifer@osumc.edu. Dawn Gullifer BS, HT (ASCP) Laboratory Supervisor OSU Histology Lab, LLC 614-293-0358 office 614-293-0345 lab dawn.gullifer@osumc.edu This e-mail, including attachments, may include confidential and/or proprietary information, and may be used only by the person or entity to which it is addressed. If the reader of this e-mail is not the intended recipient or his or her authorized agent, the reader is hereby notified that any dissemination, distribution or copying of this e-mail is prohibited. If you have received this e-mail in error, please notify the sender by responding to this e-mail and destroy immediately. ------------------------------ Message: 6 Date: Fri, 6 Jun 2008 14:33:28 -0400 From: "Gessford, Mary" Subject: RE: [Histonet] Sally's Friday Fume To: "Breeden, Sara" , Message-ID: Content-Type: text/plain; charset="us-ascii" I don't believe the rules have changed. Unless they changed in the last few weeks. I just sent verification for one of my techs to take the HTL in the last 2 months and had no problems. We are a large Pharma, our lab is all Vet Path. There are at least 21 Vet schools in the US and a State Diagnostic lab in every State in the country. My HT goes back to 1982 and my HTL and IHC are from the 2003 and 2005. The VIR group of the NSH is a large group of Registered techs. We have always been covered under the same ASCP. There is no distinction between human and animal. In fact, when grading the practical (that was discontinued) in the last 10 years or so the ASCP always had a vet path and histo tech from VIR on the grading committee. I believe Liz was a grader. You should call ASCP again and set them strait, maybe I will! Mary Gessford BA,HT,HTL(ASCP)IHC Scientist II Supervisor Anatomic Pathology Schering-Plough Summit, NJ 908-473-4358 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, June 06, 2008 12:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sally's Friday Fume Okay, I finally have something to Fume about. I am beginning to train a person to become a certified histologist. She is a certified veterinary tech with an Associates Degree and the appropriate number of chemistry/biology hours. I inquired of ASCP about her eligibility (after we finish the training period) to take the HT exam. According to ASCP, as a vet diagnostic lab, we do not meet the ASCP requirements for eligibility because we are not accredited by CAP, JCAHO/AABB and do not have a board-certified (read "human") pathologist/medical scientist (hmmm? Could one of our veterinary pathologists fulfill the description of "medical scientist"??). Surely there are other persons working in a veterinary pathology lab that would be interested in certification, one would think. What are they to do? This frosts my petunias and I'd like to know if anyone has any experience or input on this. But - hey! - some of my fluids/solutions in gallon/liter containers seem to have been improved so they don't drip, so maybe someone out there IS listening (that was my first Friday Hour of Fuming subject). Hasta lumbago. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. ------------------------------ Message: 7 Date: Fri, 6 Jun 2008 13:45:00 -0500 From: "Terry Costello" Subject: [Histonet] unsubscribe To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Thanks. Terry Costello Senior Laboratory Animal Technologist GTx, Inc 3rd Floor Van Vleet Bldg. Memphis, TN 38163 900-523-9700 ext. 102 Fax: 901-523-9772 tcostello@gtxinc.com This electronic message, including any attachments, is confidential and proprietary and is solely for the intended recipient. If you are not the intended recipient, this message was sent to you in error and you are hereby advised that any review, disclosure, copying, distribution or use of this message, or any of the information included therein, is unauthorized and strictly prohibited. If you have received this electronic transmission in error, please immediately notify the sender by reply and permanently delete all copies of this message and its attachments. ------------------------------ Message: 8 Date: Fri, 6 Jun 2008 15:00:57 EDT From: JMyers1@aol.com Subject: [Histonet] Peroxidase blocking in frozen sections To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" 'Netters: I'd like to know what reagents/methods people are using to block for endogenous peroxidase in frozen sections. Any feedback that you might be willing to offer is greatly appreciated. Joe **************Get trade secrets for amazing burgers. Watch "Cooking with Tyler Florence" on AOL Food. (http://food.aol.com/tyler-florence?video=4?&NCID=aolfod00030000000002) ------------------------------ Message: 9 Date: Fri, 6 Jun 2008 13:05:30 -0600 From: "Liz Chlipala" Subject: RE: [Histonet] Sally's Friday Fume To: "Gessford, Mary" , "Breeden, Sara" , Message-ID: Content-Type: text/plain; charset="us-ascii" Mary is right, I was a grader and also sat on the BOR for about 8 or so years back in the late 80's and 90's. There was always representation on both the Board of Registry Histotechnology Exam Committee and within the pool of individuals who were slide graders from VIR, both veterinary pathologists, HT's or HTL's that worked in research. It has always been a very balanced group of individuals consisting of techs, veterinary pathologists and MD pathologists. At least when I was invloved. In my situation here, since this is my own lab and there is no medical director or pathologist who could sign we needed to write a letter to the BOR, that's what they told us to do at the time my tech sat for the HTL and when the two of us took the IHC qualification, that was around 2002 or 2003 I think. I'm just guessing but I think that the individual who told you this info was misinformed not unless things have changed. Is there anyone out there who is on the current BOR Histotechnology exam committee comment? I just reviewed the HTL requirements on line an here is what it states, it looks like experience in any histopathology laboratory will do (am I reading this correctly?) so I think that the person you spoke to at ASCP was misinformed. To be eligible for this examination category, an applicant must satisfy the requirements of at least one of the following routes: Route 1: Baccalaureate degree from a regionally accredited college/university with a combination of 30 semester hours (45 quarter hours) of biology and chemistry AND successful completion of a NAACLS accredited Histotechnician or Histotechnology program within the last 5 years; or Route 2: Baccalaureate degree from a regionally accredited college/university with a combination of 30 semester hours (45 quarter hours) of biology and chemistry AND one year full time acceptable experience in a histopathology laboratory in the U.S., Canada or a CAP/The Joint Commission (JCAHO)/AABB accredited laboratory within the last ten years. This year of experience must be under the supervision of a pathologist (certified by the American Board of Pathology in Anatomic Pathology) or an appropriately board certified medical scientist. Clinical Laboratory Experience To fulfill the experience requirement for the Histotechnologist examination, you must have experience, within the last ten years, in the following areas: Fixation Microtomy Processing Staining Thanks Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gessford, Mary Sent: Friday, June 06, 2008 12:33 PM To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Sally's Friday Fume I don't believe the rules have changed. Unless they changed in the last few weeks. I just sent verification for one of my techs to take the HTL in the last 2 months and had no problems. We are a large Pharma, our lab is all Vet Path. There are at least 21 Vet schools in the US and a State Diagnostic lab in every State in the country. My HT goes back to 1982 and my HTL and IHC are from the 2003 and 2005. The VIR group of the NSH is a large group of Registered techs. We have always been covered under the same ASCP. There is no distinction between human and animal. In fact, when grading the practical (that was discontinued) in the last 10 years or so the ASCP always had a vet path and histo tech from VIR on the grading committee. I believe Liz was a grader. You should call ASCP again and set them strait, maybe I will! Mary Gessford BA,HT,HTL(ASCP)IHC Scientist II Supervisor Anatomic Pathology Schering-Plough Summit, NJ 908-473-4358 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, June 06, 2008 12:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sally's Friday Fume Okay, I finally have something to Fume about. I am beginning to train a person to become a certified histologist. She is a certified veterinary tech with an Associates Degree and the appropriate number of chemistry/biology hours. I inquired of ASCP about her eligibility (after we finish the training period) to take the HT exam. According to ASCP, as a vet diagnostic lab, we do not meet the ASCP requirements for eligibility because we are not accredited by CAP, JCAHO/AABB and do not have a board-certified (read "human") pathologist/medical scientist (hmmm? Could one of our veterinary pathologists fulfill the description of "medical scientist"??). Surely there are other persons working in a veterinary pathology lab that would be interested in certification, one would think. What are they to do? This frosts my petunias and I'd like to know if anyone has any experience or input on this. But - hey! - some of my fluids/solutions in gallon/liter containers seem to have been improved so they don't drip, so maybe someone out there IS listening (that was my first Friday Hour of Fuming subject). Hasta lumbago. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Fri, 6 Jun 2008 15:29:28 -0400 From: "Della Speranza, Vinnie" Subject: [Histonet] RE: Veterinary Histologist Certification Eligibility To: "'Breeden, Sara'" , "histonet@lists.utsouthwestern.edu" Cc: "'marilynng@EARTHLINK.NET'" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Sara, I certainly understand your frustration and I'm in agreement with those who've responded to your original message that the very last thing we wish to do is discourage individuals who wish to become certified. I am copying Marilyn Gamble who is the NSH representative to the Board of Registry so that she can clarify if there has been any misunderstanding during this discussion on Histonet and likewise can represent the issue to the BOR. In the days when the practical was in place, it may have been appropriate for the technician/technologist to receive guidance for the selection of appropriate tissues to satisfy the exam requirements. However, I seem to recall grading slides on animal tissues which at the time I imagined came from veterinary labs. Marilyn please "reply to all" if you can provide information that will clarify the situation. I am forwarding the earlier messages to you separately. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, June 06, 2008 2:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Veterinary Histologist Certification Eligibility That's a mouthful! Anyway - thank you to those who responded to me. I searched out a board-certified pathologist (non-veterinary) who works in our building (we share a State of NM building with OMI and the State Laboratory) and he was as astounded as I. I appreciate the feedback about writing a letter to BOR requesting a "waiver" for a veterinary situation and I will try that when the time comes. Our lab, in anticipation of our new building (2010) is operating under conditional AALVD certification but certainly not under CAP/JCHAO. I'm thinking of asking for a definition of "medical scientist" from the ASCP. The saga continues, but "where there's a will, there's a way". One would think anyone who is interested in becoming a certified histologist would receive the maximum amount of encouragement, from the employer AND the certifying agency. Perhaps it's time for a review of the requirements or at least of the definition of an employer's validity to provide appropriate training, hmmm??? Mr. Della Speranza, do you have a take on this? It is not my goal here to fluster, annoy, or pester. It's just that I'm beginning to see a certain amount of reluctance on the part of those institutions whose interest should be to encourage the growth of our profession. Now that my aim is to interest local mid-high and high school forensic science students in a detour to the Paraffin Side, I have a path littered with roadblocks. If our profession had the same PR firm that the anti-tobacco industry had, we'd be up to our earlobes in histotechs (did that come out right?). Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Fri, 06 Jun 2008 12:49:26 -0700 From: "Behnaz Sohrab" Subject: [Histonet] SLide Holder To: Message-ID: <48493246.4347.0054.0@ah.org> Content-Type: text/plain; charset=US-ASCII Any one knows where I can buy brown/black microwave able slide holder? Thanks,Behnaz Sohrab ------------------------------ Message: 12 Date: Fri, 06 Jun 2008 13:01:03 -0700 From: Patti Loykasek Subject: [Histonet] IHC neg. controls (Friday rant) To: histonet Message-ID: Content-Type: text/plain; charset="ISO-8859-1" Can someone rationalize to me the practice of running a negative control for every antibody in an IHC workup? For example, six antibodies & six negative controls??? This makes me crazy. Just had a case, needle biopsy, where this occurred at an outside institution, and now we don?t have enough tumor left to run more IHC & get a diagnosis. It borders on malpractice IMO. On the AP CAP checklist ANP.2270 Are appropriate negative controls used? The comment has the following (near the end): A negative tissue control must be processed for each antibody in a given run. Any of the following can serve as a negative tissue control: 1. Multitissue blocks. These can provide simultaneous positive and negative tissue controls, and are considered ?best practice? 2. The positive control slide or patient test slides, if these slides contain tissue elements that should not react with the antibody. 3. A separate negative tissue control slide. I think it best to asses this negative tissue control on your positive controls that should contain negative elements. Plus, use known negative elements on the patient slides. The patient tissue is precious & these patients have undergone procedures that have associated morbidity. I can?t see using up patient tissue for multiple negative controls & Having the patient have to undergo another procedure! Ok ? I?ll stop now. Patti Loykasek This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ------------------------------ Message: 13 Date: Fri, 06 Jun 2008 13:49:42 -0700 From: Patti Loykasek Subject: Re: [Histonet] IHC neg. controls (Friday rant) To: "Morken, Tim" , histonet Message-ID: Content-Type: text/plain; charset="ISO-8859-1" Hi Tim. I totally agree with you. I DO NOT think a negative should be run for each antibody. In the comment of the ANP.2270 they even mention running one for each antigen retrieval, but add if not then to use most aggressive AR & they list the AR in order of aggresiveness. Running a negative for each antibody is waste & if cited by a CAP inspector, I would appeal to CAP - especially considering the way the comment is written. I was hoping someone who is running a negative with each antibody could tell me their rationale for doing this. Patti > Patti, It does not say you need a separate negative tissue for each antibody, > only "A" negative control tissue. One of each block, and for each protocol > (ie, different pretreatments or detection system) would suffice. > > > Tim Morken > Technical Support Manager > Lab Vision Products > Anatomical Pathology > ThermoFisher Scientific > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek > Sent: Friday, June 06, 2008 1:01 PM > To: histonet > Subject: [Histonet] IHC neg. controls (Friday rant) > > Can someone rationalize to me the practice of running a negative control for > every antibody in an IHC workup? For example, six antibodies & six negative > controls??? This makes me crazy. Just had a case, needle biopsy, where this > occurred at an outside institution, and now we don?t have enough tumor left to > run more IHC & get a diagnosis. It borders on malpractice IMO. > > On the AP CAP checklist ANP.2270 Are appropriate negative controls used? The > comment has the following (near the end): > > A negative tissue control must be processed for each antibody in a given run. > Any of the following can serve as a negative tissue control: > > 1. Multitissue blocks. These can provide simultaneous positive > and negative tissue controls, and are considered ?best practice? > 2. The positive control slide or patient test slides, if these > slides contain tissue elements that should not react with the antibody. > 3. A separate negative tissue control slide. > > I think it best to asses this negative tissue control on your positive > controls that should contain negative elements. Plus, use known negative > elements on the patient slides. The patient tissue is precious & these > patients have undergone procedures that have associated morbidity. I can?t see > using up patient tissue for multiple negative controls & Having the patient > have to undergo another procedure! > Ok ? I?ll stop now. > > Patti Loykasek > > > This e-mail message, including any attachments, is for the sole use of the > intended recipients and may contain privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not the > intended recipient, please contact the sender by e-mail and destroy all copies > of the original message, or you may call PhenoPath Laboratories, Seattle, WA > U.S.A. > at (206) 374-9000. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ------------------------------ Message: 14 Date: Fri, 6 Jun 2008 14:04:57 -0700 (PDT) From: Larry Woody Subject: Re: [Histonet] IHC neg. controls (Friday rant) To: Patti Loykasek , histonet Message-ID: <806964.24310.qm@web53606.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 There could be a legal angle to it in which case wouldn't make it rational but that is the only thing I can come up with on a Friday. Patti Loykasek wrote: Can someone rationalize to me the practice of running a negative control for every antibody in an IHC workup? For example, six antibodies & six negative controls??? This makes me crazy. Just had a case, needle biopsy, where this occurred at an outside institution, and now we don?t have enough tumor left to run more IHC & get a diagnosis. It borders on malpractice IMO. On the AP CAP checklist ANP.2270 Are appropriate negative controls used? The comment has the following (near the end): A negative tissue control must be processed for each antibody in a given run. Any of the following can serve as a negative tissue control: 1. Multitissue blocks. These can provide simultaneous positive and negative tissue controls, and are considered ?best practice? 2. The positive control slide or patient test slides, if these slides contain tissue elements that should not react with the antibody. 3. A separate negative tissue control slide. I think it best to asses this negative tissue control on your positive controls that should contain negative elements. Plus, use known negative elements on the patient slides. The patient tissue is precious & these patients have undergone procedures that have associated morbidity. I can?t see using up patient tissue for multiple negative controls & Having the patient have to undergo another procedure! Ok ­ I?ll stop now. Patti Loykasek This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Larry A. Woody Seattle, Wa. ------------------------------ Message: 15 Date: Fri, 6 Jun 2008 14:07:43 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] IHC neg. controls (Friday rant) To: Patti Loykasek , histonet Message-ID: <23683.58242.qm@web65707.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 If you detection system is the same and all the procedure is the same for the 6 Abs, except for the Abs, you will need only ONE negative control per tissue, per block, but not per Ab. Ren? J. Patti Loykasek wrote: Can someone rationalize to me the practice of running a negative control for every antibody in an IHC workup? For example, six antibodies & six negative controls??? This makes me crazy. Just had a case, needle biopsy, where this occurred at an outside institution, and now we don?t have enough tumor left to run more IHC & get a diagnosis. It borders on malpractice IMO. On the AP CAP checklist ANP.2270 Are appropriate negative controls used? The comment has the following (near the end): A negative tissue control must be processed for each antibody in a given run. Any of the following can serve as a negative tissue control: 1. Multitissue blocks. These can provide simultaneous positive and negative tissue controls, and are considered ?best practice? 2. The positive control slide or patient test slides, if these slides contain tissue elements that should not react with the antibody. 3. A separate negative tissue control slide. I think it best to asses this negative tissue control on your positive controls that should contain negative elements. Plus, use known negative elements on the patient slides. The patient tissue is precious & these patients have undergone procedures that have associated morbidity. I can?t see using up patient tissue for multiple negative controls & Having the patient have to undergo another procedure! Ok ­ I?ll stop now. Patti Loykasek This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Fri, 6 Jun 2008 15:40:19 -0700 From: JR R Subject: [Histonet] RE:Peroxidase blocking in frozen sections To: Message-ID: Content-Type: text/plain; charset="Windows-1252" I use Zymed's Peroxo-Block for 30-45 seconds. Jerry Ricks Research Scientist University of Washington Department of Pathology> From: JMyers1@aol.com> Date: Fri, 6 Jun 2008 15:00:57 -0400> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] Peroxidase blocking in frozen sections> > 'Netters:> I'd like to know what reagents/methods people are using to block for > endogenous peroxidase in frozen sections. Any feedback that you might be willing to > offer is greatly appreciated.> Joe> > > > **************Get trade secrets for amazing burgers. Watch "Cooking with > Tyler Florence" on AOL Food. > (http://food.aol.com/tyler-florence?video=4?&NCID=aolfod00030000000002)> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ It's easy to add contacts from Facebook and other social sites through Windows Live(tm) Messenger. Learn how. https://www.invite2messenger.net/im/?source=TXT_EML_WLH_LearnHow ------------------------------ Message: 17 Date: Fri, 6 Jun 2008 18:13:43 -0700 (PDT) From: pruegg@ihctech.net Subject: Re: [Histonet] IHC neg. controls (Friday rant) To: "Patti Loykasek" Cc: histonet Message-ID: <1149.74.127.102.244.1212801223.squirrel@webmail.ihctech.net> Content-Type: text/plain;charset=iso-8859-1 Patti et al, this has been a bone of contention in IHC for some time now, the IHC Resource Group has discussed this year after year with no viable solution that CAP will go for, i know for a fact that many in the business save precious tissue and run just one negative reagent control for the antibody species and most agressive pretreatment for the batch, then take their chances with CAP when the time comes. Patsy > Can someone rationalize to me the practice of running a negative control > for > every antibody in an IHC workup? For example, six antibodies & six > negative > controls??? This makes me crazy. Just had a case, needle biopsy, where > this > occurred at an outside institution, and now we don?t have enough tumor > left > to run more IHC & get a diagnosis. It borders on malpractice IMO. > > On the AP CAP checklist ANP.2270 Are appropriate negative controls used? > The > comment has the following (near the end): > > A negative tissue control must be processed for each antibody in a given > run. Any of the following can serve as a negative tissue control: > > 1. Multitissue blocks. These can provide simultaneous > positive > and negative tissue controls, and are considered ?best practice? > 2. The positive control slide or patient test slides, if these > slides contain tissue elements that should not react with the antibody. > 3. A separate negative tissue control slide. > > I think it best to asses this negative tissue control on your positive > controls that should contain negative elements. Plus, use known negative > elements on the patient slides. The patient tissue is precious & these > patients have undergone procedures that have associated morbidity. I can?t > see using up patient tissue for multiple negative controls & Having the > patient have to undergo another procedure! > Ok ? I?ll stop now. > > Patti Loykasek > > > This e-mail message, including any attachments, is for the sole use of the > intended recipients and may contain privileged information. Any > unauthorized > review, use, disclosure or distribution is prohibited. If you are not the > intended > recipient, please contact the sender by e-mail and destroy all copies of > the > original message, or you may call PhenoPath Laboratories, Seattle, WA > U.S.A. > at (206) 374-9000. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 18 Date: Fri, 06 Jun 2008 21:14:38 -0400 From: "Andrea Hooper" Subject: [Histonet] pAKT To: Histonet Message-ID: Content-Type: text/plain; charset=us-ascii; format=flowed What Abs are people using for pAKT these days? The one I had working so well in FFPE is no longer being made by Cell Signaling. Thanks, Andrea -- ------------------------------ Message: 19 Date: Fri, 6 Jun 2008 18:21:08 -0700 (PDT) From: pruegg@ihctech.net Subject: Re: [Histonet] pAKT To: "Andrea Hooper" Cc: Histonet Message-ID: <1164.74.127.102.244.1212801668.squirrel@webmail.ihctech.net> Content-Type: text/plain;charset=iso-8859-1 cell signaling has a couple of alternative abs for pAKT they claim are better, i believe they are rab monoclonals, i have not tried them yet. Patsy > What Abs are people using for pAKT these days? The one I had working > so well in FFPE is no longer being made by Cell Signaling. > > Thanks, > Andrea > -- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 20 Date: Fri, 6 Jun 2008 19:56:13 -0600 From: "Janet Maass" Subject: Re: [Histonet] Sally's Friday Fume To: "Breeden, Sara" , Message-ID: <004201c8c841$aaba1390$0200a8c0@Janet03b999f> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Hi Sara I think this was an error on the part of the individual that you communicated with at ASCP. While I served on the HT/HTL Committee for two terms, I worked in veterinary histology. Also on The Committee was a veterinary pathologist for two terms. While owning my own veterinary histology consulting lab, I trained several people who took there certification exam. Currently on The Committee is someone that is working in veterinary histology. Janet Maass ------------------------------ Message: 21 Date: Sat, 7 Jun 2008 09:27:18 -0500 From: "Scott, Allison D" Subject: [Histonet] Disposing of used paraffin from the tissue processor To: Message-ID: <1872B4A455B7974391609AD8034C79FC082E1D@LBEXCH01.hchd.local> Content-Type: text/plain; charset="iso-8859-1" Hello to all in histoland. How are other facilities disposing of used paraffin from the tissue processor. I have a box that I have lined with several red biohazrd bag and I pour all of the used paraffin in it. I let it harden, and after about a week I pull out the hard paraffin and put it in a biohazard box that has a biohazard bag in it also, close it up and it is ready for our environmental services people to pick up and discard. I would not think that this paraffin can be put into the regular trash since tissues and chemicals have been through it. Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ------------------------------ Message: 22 Date: Sat, 7 Jun 2008 08:44:39 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Disposing of used paraffin from the tissue processor To: "Scott, Allison D" , histonet@lists.utsouthwestern.edu Message-ID: <136676.61962.qm@web65702.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 We used to incinerate it. Ren? J. "Scott, Allison D" wrote: Hello to all in histoland. How are other facilities disposing of used paraffin from the tissue processor. I have a box that I have lined with several red biohazrd bag and I pour all of the used paraffin in it. I let it harden, and after about a week I pull out the hard paraffin and put it in a biohazard box that has a biohazard bag in it also, close it up and it is ready for our environmental services people to pick up and discard. I would not think that this paraffin can be put into the regular trash since tissues and chemicals have been through it. Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 23 Date: Sat, 7 Jun 2008 09:53:23 -0700 (PDT) From: Linda Subject: Re: [Histonet] NSH Histology Task Analysis and StandardsofPerformance Competen cy To: Norm Burnham , "Rathborne, Toni" , "Mahoney,Janice A" , "McKnight, Tanisha" , histonet@lists.utsouthwestern.edu Message-ID: <30657.26774.qm@web36503.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I would be most greatfull for a copy too. Linda Dee Chicago, IL ----- Original Message ---- From: Norm Burnham To: "Rathborne, Toni" ; "Mahoney,Janice A" ; "McKnight, Tanisha" ; histonet@lists.utsouthwestern.edu Sent: Friday, June 6, 2008 10:48:33 AM Subject: RE: [Histonet] NSH Histology Task Analysis and StandardsofPerformance Competen cy Me too!? Norm Burnham -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, June 06, 2008 10:27 AM To: Mahoney,Janice A; McKnight, Tanisha; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH Histology Task Analysis and StandardsofPerformance Competen cy As would I. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Mahoney,Janice A Sent: Friday, June 06, 2008 11:23 AM To: 'McKnight, Tanisha'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH Histology Task Analysis and Standards ofPerformance Competen cy I'd love to have an electronic copy as well Jan Mahoney Alegent Health Omaha,NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McKnight, Tanisha Sent: Friday, June 06, 2008 10:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NSH Histology Task Analysis and Standards of Performance Competen cy Hello Histo-netters: Would anyone happen to have an electronic copy of NSH Histology Task Analysis and The Standards of Performance/Competency that you could send to me? I am in desperate need of these items. I've contacted NSH for these twice, but I have gotten no response. I'd greatly appreciate anything you could send. Tanisha N. McKnight, HT (ASCP) Covance CLS Indianapolis Specimen Management, Anatomic Pathology ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. 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Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. ______________________________________________________________________________ This e-mail may contain confidential or privileged information. If you think you have received this e-mail in error, please advise the sender by reply e-mail and then delete this e-mail immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 55, Issue 13 **************************************** From tanisha.mcknight <@t> covance.com Tue Jun 10 09:11:09 2008 From: tanisha.mcknight <@t> covance.com (McKnight, Tanisha) Date: Tue Jun 10 09:11:35 2008 Subject: [Histonet] Advice on Training Histology Lab Assistants Message-ID: <816E3C72F855F14985FC31D7C963AE6F0774785D@indexch03.ent.covance.com> Hello All: I have a few people, now working in accessioning, who are interested in working in Histology. I am thinking of potentially creating "Histology Lab Assistant" positions to help them transition. They have already been told that they will need to go through an accredited program to become full Techs. We have one here in Indiana that I went through and it is great. Can you all share your strategies for training? How do you separate what Assistants are allowed to do from what Techs do? What regulatory guidelines do you follow if any when deciding? I was thinking of training them to embed and create sections first (on limited specimen types). I would not allow certain biopsies or really small specimens. Under regulations, would sectioning and embedding be considered "testing"? Any help or advice would be appreciated. Tanisha N. McKnight, HT (ASCP) Covance CLS Indianapolis Specimen Management, Anatomic Pathology ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From TMcNemar <@t> lmhealth.org Tue Jun 10 10:01:46 2008 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Tue Jun 10 10:01:50 2008 Subject: [Histonet] Advice on Training Histology Lab Assistants In-Reply-To: <816E3C72F855F14985FC31D7C963AE6F0774785D@indexch03.ent.covance.com> Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F582@lmhsmail.lmhealth.org> Hello, I have trained a couple of Assistants. All they do here is accession and assist the pathologist with gross, enter and process Cytologies, coverslip, file slides and block, and change stainers. That keeps them pretty busy. They do not do special stains, cut, or do frozens. Not because I feel that they cannot be taught to do those things, but you need some tasks excluded to justify the assistant's lower pay range or the tech's high range, depending on how you choose to look at it. You don't want to pay them less then ask them to do the more skilled tasks. As for embedding, I am very particular about that and would never give that job to an assistant. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of McKnight, Tanisha Sent: Tuesday, June 10, 2008 10:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Advice on Training Histology Lab Assistants Hello All: I have a few people, now working in accessioning, who are interested in working in Histology. I am thinking of potentially creating "Histology Lab Assistant" positions to help them transition. They have already been told that they will need to go through an accredited program to become full Techs. We have one here in Indiana that I went through and it is great. Can you all share your strategies for training? How do you separate what Assistants are allowed to do from what Techs do? What regulatory guidelines do you follow if any when deciding? I was thinking of training them to embed and create sections first (on limited specimen types). I would not allow certain biopsies or really small specimens. Under regulations, would sectioning and embedding be considered "testing"? Any help or advice would be appreciated. Tanisha N. McKnight, HT (ASCP) Covance CLS Indianapolis Specimen Management, Anatomic Pathology ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From rjbuesa <@t> yahoo.com Tue Jun 10 10:17:59 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jun 10 10:18:03 2008 Subject: [Histonet] Advice on Training Histology Lab Assistants In-Reply-To: <816E3C72F855F14985FC31D7C963AE6F0774785D@indexch03.ent.covance.com> Message-ID: <433454.38297.qm@web65706.mail.ac4.yahoo.com> I have always applied a "rule of thumb" to determine what a lab assistant can or cannot do: "IF whatever is going to be done relates with the SPECIMEN in a way that the specimen can be altered/damaged in any irreversible way, then it is NOT a task for the assistant". Examples: description, cassetting, embedding or sectioning. Ren? J. "McKnight, Tanisha" wrote: Hello All: I have a few people, now working in accessioning, who are interested in working in Histology. I am thinking of potentially creating "Histology Lab Assistant" positions to help them transition. They have already been told that they will need to go through an accredited program to become full Techs. We have one here in Indiana that I went through and it is great. Can you all share your strategies for training? How do you separate what Assistants are allowed to do from what Techs do? What regulatory guidelines do you follow if any when deciding? I was thinking of training them to embed and create sections first (on limited specimen types). I would not allow certain biopsies or really small specimens. Under regulations, would sectioning and embedding be considered "testing"? Any help or advice would be appreciated. Tanisha N. McKnight, HT (ASCP) Covance CLS Indianapolis Specimen Management, Anatomic Pathology ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MLinville <@t> ameripath.com Tue Jun 10 10:52:45 2008 From: MLinville <@t> ameripath.com (Linville, Marsha) Date: Tue Jun 10 10:52:50 2008 Subject: [Histonet] NSH Histology Task Analysis and StandardsofPerformanceCompeten cy In-Reply-To: <8CEB6DA1A3F35743800669D4CFE21F7D03F90086@NSMXMS04.northside.local> References: <30657.26774.qm@web36503.mail.mud.yahoo.com> <8CEB6DA1A3F35743800669D4CFE21F7D03F90086@NSMXMS04.northside.local> Message-ID: <8613338BCE81D0438B9823A8AF6BEE2D04BBB8F9@TXCLMAIL01.ameripath.local> I would appreciate a copy as well. Marsha Linville Histology Supervisor AmeriPath Indiana Phone: (317)275-8140 Fax: (317)275-8070 Pager: (317)368-1534 -----Original Message----- From: Terri Brown [mailto:Terri.Brown@Northside.com] Sent: Monday, June 09, 2008 3:04 PM To: Linda; Norm Burnham; Rathborne, Toni; Mahoney,Janice A; McKnight, Tanisha; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH Histology Task Analysis and StandardsofPerformanceCompeten cy I would like a copy too. Terri H. Brown Pathology Laboratory Manager Northside Hospital Office: 404-845-5423 Fax: 404-851-6400 terri.brown@northside.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Sent: Saturday, June 07, 2008 12:53 PM To: Norm Burnham; Rathborne, Toni; Mahoney,Janice A; McKnight, Tanisha; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] NSH Histology Task Analysis and StandardsofPerformance Competen cy I would be most greatfull for a copy too. Linda Dee Chicago, IL ----- Original Message ---- From: Norm Burnham To: "Rathborne, Toni" ; "Mahoney,Janice A" ; "McKnight, Tanisha" ; histonet@lists.utsouthwestern.edu Sent: Friday, June 6, 2008 10:48:33 AM Subject: RE: [Histonet] NSH Histology Task Analysis and StandardsofPerformance Competen cy Me too!? Norm Burnham -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, June 06, 2008 10:27 AM To: Mahoney,Janice A; McKnight, Tanisha; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH Histology Task Analysis and StandardsofPerformance Competen cy As would I. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Mahoney,Janice A Sent: Friday, June 06, 2008 11:23 AM To: 'McKnight, Tanisha'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NSH Histology Task Analysis and Standards ofPerformance Competen cy I'd love to have an electronic copy as well Jan Mahoney Alegent Health Omaha,NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McKnight, Tanisha Sent: Friday, June 06, 2008 10:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NSH Histology Task Analysis and Standards of Performance Competen cy Hello Histo-netters: Would anyone happen to have an electronic copy of NSH Histology Task Analysis and The Standards of Performance/Competency that you could send to me? I am in desperate need of these items. I've contacted NSH for these twice, but I have gotten no response. I'd greatly appreciate anything you could send. Tanisha N. McKnight, HT (ASCP) Covance CLS Indianapolis Specimen Management, Anatomic Pathology ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful.? If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer.? Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening.? Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail.? Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law.? Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.? If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. ______________________________________________________________________________ This e-mail may contain confidential or privileged information. If you think you have received this e-mail in error, please advise the sender by reply e-mail and then delete this e-mail immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. From amber.mckenzie <@t> gastrodocs.net Tue Jun 10 11:18:45 2008 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Tue Jun 10 11:18:52 2008 Subject: [Histonet] NSH Histology Task Analysis and Standards of Performance Competency In-Reply-To: <816E3C72F855F14985FC31D7C963AE6F0491B6AE@indexch03.ent.covance.com> Message-ID: <03C921A1EAF7F541B16543F6EC6A4B3701BF248B@giamail2.Gia.com> I email NSH yesterday and have already gotten a response with the requested material - Histology Task Analysis and the Standards of Performance. Very simple, easy and fast. Amber -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McKnight, Tanisha Sent: Monday, June 09, 2008 7:36 PM To: 'Della Speranza, Vinnie '; 'histonet@lists.utsouthwestern.edu ' Subject: RE: [Histonet] NSH Histology Task Analysis and Standards of Performance Competen cy Hello Vinnie: Thank you for responding to this matter. I'd like to assure you that I am indeed a member of NSH. You may want to conduct your search under my unmarried name Tanisha Neely. It was not my intent to cause a problem or imply something negative about NSH. I love the work that you do and I would encourage anyone in our field to become a member. However, I have sent 2 separate requests for these items by email and made one request by phone (I was told the person who handled this wasn't in). I have yet to receive a response. Thank you again for yours. Sincerely, Tanisha Neely McKnight -----Original Message----- From: Della Speranza, Vinnie To: McKnight, Tanisha; histonet@lists.utsouthwestern.edu Sent: 6/9/2008 5:40 PM Subject: RE: [Histonet] NSH Histology Task Analysis and Standards of Performance Competen cy In reviewing the messages of this thread I came across Tanisha's message. I'd like to reiterate that the Task Analysis is available only to NSH members. Tanisha's message gives the impression that NSH has been unresponsive to her. I am writing to clear the record and to assist her. Tanisha is not listed as a current member according to the NSH member directory. If this information is incorrect Tanisha, please contact the NSH office to have these records corrected. If you are unsuccessful in getting this issue resolved, I invite you to phone me directly and I will be happy to intercede on your behalf. I've listed my phone number in my signature at the bottom of these remarks. For those of you who might find the Task Analysis of value but are not members, I would encourage you to consider affiliating with the society. Dues are very affordable ($60) and there are many resources available to members that you will find desirable and helpful to you in your work. We have many programs and are working diligently on behalf of NSH members throughout the year. We are happy to assist you in meeting your professional goals. Vinnie Della Speranza President, National Society for Histotechnology (843) 792-6353 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McKnight, Tanisha Sent: Friday, June 06, 2008 11:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NSH Histology Task Analysis and Standards of Performance Competen cy Hello Histo-netters: Would anyone happen to have an electronic copy of NSH Histology Task Analysis and The Standards of Performance/Competency that you could send to me? I am in desperate need of these items. I've contacted NSH for these twice, but I have gotten no response. I'd greatly appreciate anything you could send. Tanisha N. McKnight, HT (ASCP) Covance CLS Indianapolis Specimen Management, Anatomic Pathology ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMyers1 <@t> aol.com Tue Jun 10 11:32:07 2008 From: JMyers1 <@t> aol.com (JMyers1@aol.com) Date: Tue Jun 10 11:32:14 2008 Subject: [Histonet] IHC on frozen skin and lung Message-ID: All: First, thanks to everyone who responded to my inquiry last Friday regarding methods of blocking for endogenous peroxidase in frozen tissues. Now, since I continue to have difficulty optimizing a few procedures, I could use some input on creating protocols for staining of frozen skin for HMB45, as well as for Pan-CK on frozen lung tissue. Thanks, Joe Myers, M.S., CT(ASCP) **************Vote for your city's best dining and nightlife. City's Best 2008. (http://citysbest.aol.com?ncid=aolacg00050000000102) From blord <@t> swmail.sw.org Tue Jun 10 11:41:21 2008 From: blord <@t> swmail.sw.org (Barbara Lord) Date: Tue Jun 10 11:41:37 2008 Subject: [Histonet] NSH Histology Task Analysis and StandardsofPerformance Competen cy Message-ID: I would like a copy also: Thanks so much Barbara Lord HT(ASCP) Department of Dermatology Mohs Tech Scott & White Hospital 409 W Adams Temple, TX 76501 From EdieL <@t> fmchealth.org Tue Jun 10 11:42:36 2008 From: EdieL <@t> fmchealth.org (Edie Lehman) Date: Tue Jun 10 11:42:40 2008 Subject: [Histonet] Automated Slide Stainer Message-ID: <3C25F9EF8E8DF84DBBB1884C297E8AF403790788@ex03.fmchealth.org> I am new to the Histology Dept. at my hospital, and have not worked in histo for many years. We are currently researching a new automated H&E stainer. The techs like the old Sakura model we currently have. We seldom have any problems with it. I have tried to find the newest model on line, however have not been successful. I can easily find the Sakura website, but cannot access the "Americas" site. Does anyone have any suggestions as to how I can get some information on this? Any suggestions for other models that have proved successful in other labs is also greatly appreciated. Thanks in advance, Edie Lehman MT(ASCP) Anatomical Pathology Supervisor Fairfield Medical Center EdieL@fmchealth.org (740)687-8807 "Confidentiality Notice: This e-mail message, including any attachments is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review; use; disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message." From jqb7 <@t> cdc.gov Tue Jun 10 11:56:05 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Tue Jun 10 11:56:20 2008 Subject: [Histonet] Automated Slide Stainer In-Reply-To: <3C25F9EF8E8DF84DBBB1884C297E8AF403790788@ex03.fmchealth.org> References: <3C25F9EF8E8DF84DBBB1884C297E8AF403790788@ex03.fmchealth.org> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A7F23B0F@LTA3VS011.ees.hhs.gov> Under "Products" go to Prisma/Film Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edie Lehman Sent: Tuesday, June 10, 2008 12:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated Slide Stainer I am new to the Histology Dept. at my hospital, and have not worked in histo for many years. We are currently researching a new automated H&E stainer. The techs like the old Sakura model we currently have. We seldom have any problems with it. I have tried to find the newest model on line, however have not been successful. I can easily find the Sakura website, but cannot access the "Americas" site. Does anyone have any suggestions as to how I can get some information on this? Any suggestions for other models that have proved successful in other labs is also greatly appreciated. Thanks in advance, Edie Lehman MT(ASCP) Anatomical Pathology Supervisor Fairfield Medical Center EdieL@fmchealth.org (740)687-8807 "Confidentiality Notice: This e-mail message, including any attachments is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review; use; disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Tue Jun 10 12:08:49 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Jun 10 12:02:51 2008 Subject: [Histonet] Automated Slide Stainer In-Reply-To: <3C25F9EF8E8DF84DBBB1884C297E8AF403790788@ex03.fmchealth.org> References: <3C25F9EF8E8DF84DBBB1884C297E8AF403790788@ex03.fmchealth.org> Message-ID: <1F937FB30BDB7C4A9F39F83FEA8D379F9F5595@bruexchange1.digestivespecialists.com> I absolutely LOVE my Tribune stainer from Surgipath! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edie Lehman Sent: Tuesday, June 10, 2008 12:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated Slide Stainer I am new to the Histology Dept. at my hospital, and have not worked in histo for many years. We are currently researching a new automated H&E stainer. The techs like the old Sakura model we currently have. We seldom have any problems with it. I have tried to find the newest model on line, however have not been successful. I can easily find the Sakura website, but cannot access the "Americas" site. Does anyone have any suggestions as to how I can get some information on this? Any suggestions for other models that have proved successful in other labs is also greatly appreciated. Thanks in advance, Edie Lehman MT(ASCP) Anatomical Pathology Supervisor Fairfield Medical Center EdieL@fmchealth.org (740)687-8807 "Confidentiality Notice: This e-mail message, including any attachments is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review; use; disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histology.bc <@t> shaw.ca Tue Jun 10 12:21:18 2008 From: histology.bc <@t> shaw.ca (Histonet) Date: Tue Jun 10 12:27:19 2008 Subject: [Histonet] Advice on Training Histology Lab Assistants In-Reply-To: <816E3C72F855F14985FC31D7C963AE6F0774785D@indexch03.ent.covance.com> References: <816E3C72F855F14985FC31D7C963AE6F0774785D@indexch03.ent.covance.com> Message-ID: <484EB80E.8090405@shaw.ca> Hi Tanisha, Here in Canada, Medical Laboratory Assistants have been used as a vital part of the laboratory workforce for many years. The national certifying body, the Canadian Society for Medical Laboratory Sciences, has adopted the MLA group and has established a National Syllabus of Studies and required competencies for each laboratory discipline. By standardizing the required competencies and the training of MLA's, it is possible to clearly define the precise role and duties that an MLA is permitted to perform. The duties that MLA's perform have been specifically chosen to avoid any erosion of responsibilities from the technologist's duties. In practice, certified technologists can perform any and all duties in the laboratory, whereas MLA's may perform only specific tasks. MLA's are paid on a lower scale than a certified technologist to reflect their more limited responsibilities. The separation of technologist's and assistant's duties is vital, especially in a situation where employers may be tempted to employ the less expensive of the two groups in order to cut their costs. Medical Laboratory Assistants are permitted to work in the gross room, accessioning specimens, entering data into the computer, labeling cassettes, filing and retrieving specimens, filing and retrieving blocks. The assistants also maintain the tissue processors and fluid levels. However, gross descriptions, specimen selection and dissection are the sole responsibility of the technologists. Embedding, sectioning, frozen sections, and staining are also the responsibility of the technologists. By assigning the more mundane, but still critical, tasks to less qualified personnel, the technologists are available to concentrate on the more demanding procedures. This system has worked very well and very effectively for many years. There is a distance education course for Histology Assistants offered through the Open Learning Division of Thompson Rivers University of British Columbia. This is specifically written for workers who have no prior knowledge of histology. It focuses on "pre-analytical procedures" but also provides a good deal of background information on diagnostic histopathology, tissues, fixation, tissues types, common specimens, tissue processing, filing, storage. It also covers some aspects of specimen preparation for cytology specimens, fluids, aspirates, etc. http://www.tru.ca/distance/programs/health_sci/medlabasst/courses.html#mlap161 The Open Learning course is available to anyone, anywhere, and may be completed within a very flexible time frame. The current cost of the course is $400.00, including all course materials, examinations, on-line tutors, and toll-free tutor phone calls. Paul Bradbury, Kamloops, Canada ************************************************************************************************************* McKnight, Tanisha wrote: > Hello All: > > I have a few people, now working in accessioning, who are interested in > working in Histology. I am thinking of potentially creating "Histology > Lab Assistant" positions to help them transition. They have already been > told that they will need to go through an accredited program to become > full Techs. We have one here in Indiana that I went through and it is > great. > > Can you all share your strategies for training? How do you separate what > Assistants are allowed to do from what Techs do? What regulatory > guidelines do you follow if any when deciding? > > I was thinking of training them to embed and create sections first (on > limited specimen types). I would not allow certain biopsies or really > small specimens. Under regulations, would sectioning and embedding be > considered "testing"? > > Any help or advice would be appreciated. > > Tanisha N. McKnight, HT (ASCP) > Covance CLS Indianapolis > Specimen Management, Anatomic Pathology > > > > > ----------------------------------------------------- > Confidentiality Notice: This e-mail transmission > may contain confidential or legally privileged > information that is intended only for the individual > or entity named in the e-mail address. If you are not > the intended recipient, you are hereby notified that > any disclosure, copying, distribution, or reliance > upon the contents of this e-mail is strictly prohibited. > > If you have received this e-mail transmission in error, > please reply to the sender, so that we can arrange > for proper delivery, and then please delete the message > from your inbox. Thank you. > > > ------------------------------------------------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gvdobbin <@t> ihis.org Tue Jun 10 12:37:21 2008 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Tue Jun 10 12:37:35 2008 Subject: [Histonet] Question for my Canadian colleagues Message-ID: Hi folks, I am conducting an informal survey to find out how other labs are staffed (believing mine to be understaffed). Could you please provide me with the following numbers: No. of pathologists/residents: No. of techs: No. of Assistants: No. of surgicals done annually (round to nearest 1000): No. of autopsies: Thanks in advance for any assistance you may be able to offer in this regard. Sincerely, Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 There is some merit in doing the right thing rather badly, but absolutely none in doing the wrong thing excellently! ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- From Stephen.Clark1 <@t> hcahealthcare.com Tue Jun 10 12:48:11 2008 From: Stephen.Clark1 <@t> hcahealthcare.com (Clark Stephen - Myrtle Beach) Date: Tue Jun 10 12:48:20 2008 Subject: [Histonet] Slide printer Message-ID: <28E40C736ED32341BBB2B094EFD23FC8020F0711@NASEV05.hca.corpad.net> I was wondering what most labs are using to label their slides. We've been using a DOT matrix printer for forever and it's finally about to die. My IT person asked me to check with other labs and see what's being implemented in the way of laser printers. Any help would be appreciated. Steve Clark Histology Supervisor Grand Strand Reg. Medical Ctr. Myrtle Beach, SC (843) 692-1486 Stephen.Clark1@hcahealthcare.com From rfields <@t> gidocs.net Tue Jun 10 12:57:31 2008 From: rfields <@t> gidocs.net (Rosa Fields) Date: Tue Jun 10 12:57:37 2008 Subject: [Histonet] Slide printer In-Reply-To: <28E40C736ED32341BBB2B094EFD23FC8020F0711@NASEV05.hca.corpad.net> References: <28E40C736ED32341BBB2B094EFD23FC8020F0711@NASEV05.hca.corpad.net> Message-ID: <2F2611250DCD6549AA3D96CE8AF1F01801124072@giexchange.gidocs.net> We have just gotten a slide etcher from Thermo, manufactured by RA Lamb, very simple to use, no ink! Small footprint to boot (no pun intended!) Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clark Stephen - Myrtle Beach Sent: Tuesday, June 10, 2008 12:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide printer I was wondering what most labs are using to label their slides. We've been using a DOT matrix printer for forever and it's finally about to die. My IT person asked me to check with other labs and see what's being implemented in the way of laser printers. Any help would be appreciated. Steve Clark Histology Supervisor Grand Strand Reg. Medical Ctr. Myrtle Beach, SC (843) 692-1486 Stephen.Clark1@hcahealthcare.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Tue Jun 10 13:08:06 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Tue Jun 10 13:08:12 2008 Subject: [Histonet] Slide printer In-Reply-To: <28E40C736ED32341BBB2B094EFD23FC8020F0711@NASEV05.hca.corpad.net> References: <28E40C736ED32341BBB2B094EFD23FC8020F0711@NASEV05.hca.corpad.net> Message-ID: <484EC306.3050400@pathology.washington.edu> If you are still going to use some form of a paper label, I recommend using a Zebra printer. Due to space we are using the TLP3844Z which is a small desktop printer that prints out 500+ labels a day. Very robust and trouble free printing. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Clark Stephen - Myrtle Beach wrote: > I was wondering what most labs are using to label their slides. We've > been using a DOT matrix printer for forever and it's finally about to > die. My IT person asked me to check with other labs and see what's > being implemented in the way of laser printers. Any help would be > appreciated. > > > > Steve Clark > > Histology Supervisor > > Grand Strand Reg. Medical Ctr. > > Myrtle Beach, SC > > (843) 692-1486 > > Stephen.Clark1@hcahealthcare.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From arvidsonkristen <@t> yahoo.com Tue Jun 10 13:10:43 2008 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Tue Jun 10 13:10:50 2008 Subject: [Histonet] missing melanocytes?? Message-ID: <505093.96121.qm@web65712.mail.ac4.yahoo.com> Help!! Our pathologists are starting to see a new artifact where they are unable to see the melanocytes (we are all derm). It only happens on a rare occasion. We checked the PH of the water on the stainer and it seemed fine. We can't think of anything else? Help! From renafail <@t> bellsouth.net Tue Jun 10 13:16:03 2008 From: renafail <@t> bellsouth.net (Rena Fail) Date: Tue Jun 10 13:16:05 2008 Subject: [Histonet] missing melanocytes?? In-Reply-To: <505093.96121.qm@web65712.mail.ac4.yahoo.com> Message-ID: <000201c8e381$ef850e20$0301a8c0@RENAD4YK9B8ABE> Did you also check the pH of your stain solutions? Rena Fail -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kristen arvidson Sent: Tuesday, June 10, 2008 2:11 PM To: histonet Subject: [Histonet] missing melanocytes?? Help!! Our pathologists are starting to see a new artifact where they are unable to see the melanocytes (we are all derm). It only happens on a rare occasion. We checked the PH of the water on the stainer and it seemed fine. We can't think of anything else? Help! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KLAPANO1 <@t> hfhs.org Tue Jun 10 14:45:28 2008 From: KLAPANO1 <@t> hfhs.org (Karen Lapanowski) Date: Tue Jun 10 14:46:01 2008 Subject: [Histonet] VEGF Message-ID: <484EA1980200002800006B91@gwia2v.net.hfh.edu> Hello, Does anyone have a protocol for staining VEGF in fixed 100 micron vibratome rat brain sections? Thanks, Karen Karen Lapanowski Henry Ford Health System Radiation Oncology 3065 E & R 313-916-9386 ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== From histology.bc <@t> shaw.ca Tue Jun 10 15:23:50 2008 From: histology.bc <@t> shaw.ca (Histonet) Date: Tue Jun 10 15:24:19 2008 Subject: [Histonet] missing melanocytes?? In-Reply-To: <505093.96121.qm@web65712.mail.ac4.yahoo.com> References: <505093.96121.qm@web65712.mail.ac4.yahoo.com> Message-ID: <484EE2D6.6090904@shaw.ca> You say "they are unable to see the melanocytes". Or is it just the melanin granules that are "missing"? Are other cells and structures staining normally? Do the granules appear if you use Masson-Fontan or some other melanin demonstration technique? Do the problem specimens all come from the same source (clinic, surgeon, etc). Are you sure you don't have a population of albinos in your region? Paul Bradbury Kamloops, Canada ? kristen arvidson wrote: > Help!! Our pathologists are starting to see a new artifact where they are unable to see the melanocytes (we are all derm). It only happens on a rare occasion. We checked the PH of the water on the stainer and it seemed fine. We can't think of anything else? Help! > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From tkngflght <@t> yahoo.com Tue Jun 10 19:18:33 2008 From: tkngflght <@t> yahoo.com (Cheryl) Date: Tue Jun 10 19:18:33 2008 Subject: [Histonet] Need a Mohs temp in NYC Message-ID: <003b01c8cb58$b00d2070$360aa8c0@FULLSTAFF.ORG> Hi All! I need to find a Mohs tech for alternating Mondays starting ASAP (of course :). We could work out a short term situations for a couple of weeks or you could take it on as an ongoing PRN employee. Please call if you have any questions-- Cheryl Cheryl R. Kerry, HT(ASCP), BA Full Staff Inc. Staffing the lab - One GREAT tech at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone admin@fullstaff.org www.fullstaff.org From swelam48 <@t> gmail.com Tue Jun 10 22:16:01 2008 From: swelam48 <@t> gmail.com (wael swelam) Date: Tue Jun 10 22:16:14 2008 Subject: [Histonet] Antibody against ED-B domain of Fibronectin Message-ID: <96566f7e0806102016w381b400esf1c799595d378091@mail.gmail.com> Dear Histonetters, I'm looking for anti-human antibody against Fibronectin extracellular domain B. Your help will be appreciated. Yours, Wael Mohamed Swelam BDS (Egypt), Oral Pathology MSc (Egypt), Oral Pathology PhD (Japan), Ass. Prof. Oral Biomedical Science dept. Division of Oral Pathology, Faculty of Dentistry, King Faisal University, Dammam, Saudi Arabia From Terry.Marshall <@t> rothgen.nhs.uk Wed Jun 11 07:13:44 2008 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Wed Jun 11 07:13:54 2008 Subject: [Histonet] Question for my Canadian colleagues Message-ID: <5C0BED61F529364E86309CADEA63FEF20163F41F@TRFT-EX01.xRothGen.nhs.uk> No. of pathologists/residents: 3 No. of techs: 3 No. of Assistants: 2 No. of surgicals done annually (round to nearest 1000): 16000 No. of autopsies: 740 Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Greg Dobbin Sent: 10 June 2008 18:37 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Question for my Canadian colleagues Hi folks, I am conducting an informal survey to find out how other labs are staffed (believing mine to be understaffed). Could you please provide me with the following numbers: No. of pathologists/residents: 3 No. of techs: 3 No. of Assistants: 2 No. of surgicals done annually (round to nearest 1000): 16000 No. of autopsies: 740 Thanks in advance for any assistance you may be able to offer in this regard. Sincerely, Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 There is some merit in doing the right thing rather badly, but absolutely none in doing the wrong thing excellently! ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Erin.Martin <@t> ucsf.edu Wed Jun 11 07:57:07 2008 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Wed Jun 11 07:57:26 2008 Subject: [Histonet] Fite stain Message-ID: Good morning everyone, I am having a problem with my Fite stains. Most stain beautifully but I have had trouble with a few cases. Here are the doc's comments: "case xxxx has some lipomembranous change- and this may not really be an entirely false positive result. But there is clearly no explanation for the stuff in xxxx that stains, including some fibrin. I've never seen this before. It's not the usual round spots from inadequately rinsing off the peanut oil...." "A couple of days ago, I saw a granulomatous reaction that had tiny Fite positive "blobbos" that looked like microbes in tiny cystic spaces within the granulomas. I initially thought they might be real organisms but then I did a TB stain and proved that they were nothing. Just moments ago, XXXX had a case that was essentially identical. The Fite stain is working perfectly for ID of microbes, but it seems to have a false positive kind of reactivity that can be seen in small spaces within a granulomatous infiltrate. On an occasional basis only." Does anyone have any idea? Our procedure is 2 changes of xylene/peanut oil, air dry, rinse, carbol fuchsin for 15 min, rinse, differentiate in 1% acid alcohol, rinse, counterstain in methylene blue, air dry. Thanks in advance, From jennifer.l.hofecker <@t> Vanderbilt.Edu Wed Jun 11 08:23:32 2008 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Wed Jun 11 08:23:38 2008 Subject: [Histonet] NOTCH3 Antibody Message-ID: <898D946569A27444B65667A49C0740520175B600@mailbe06.mc.vanderbilt.edu> Good Morning Everyone! I am hoping that somebody out there is successfully staining for the NOTCH 3 Protein. Specifically, I am looking for an antibody to use on FFPE (human) skin biopsies. These would be suspected CADASIL patients. The clinician would like us to use a monoclonal antibody. After several searches, I am able to find some rabbit polyclonal antibodies for use in IHC, but no monoclonals. I don't mind using a rabbit poly but there are also at least 4 clones that I've found. If any of you have an antibody that you like, please let me know. Any and all NOTCH 3 users are welcome to share your experiences! When histonet archives yield "0 Results", you know it's going to be interesting... Thanks in Advance! Jennifer L. Hofecker HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph 615.343.0083 fax 615.343.7089 From kgrobert <@t> rci.rutgers.edu Wed Jun 11 08:49:20 2008 From: kgrobert <@t> rci.rutgers.edu (Kathleen Roberts) Date: Wed Jun 11 08:39:43 2008 Subject: [Histonet] missing melanocytes?? In-Reply-To: <484EE2D6.6090904@shaw.ca> References: <505093.96121.qm@web65712.mail.ac4.yahoo.com> <484EE2D6.6090904@shaw.ca> Message-ID: <484FD7E0.7000301@rci.rutgers.edu> There is such a thing as "amelanotic melanoma"...since this only happens on a rare occasion, maybe this is it? I would think that the pathologists would know, though. (We have a professor here who studies both melanoma and the amelanotic variety, and I did the histo for her for a while-that's how I know about it.) Just a thought. Kathleen Roberts Rutgers University, NJ Histonet wrote: > You say "they are unable to see the melanocytes". Or is it just the > melanin granules that are "missing"? Are other cells and structures > staining normally? Do the granules appear if you use Masson-Fontan or > some other melanin demonstration technique? Do the problem specimens > all come from the same source (clinic, surgeon, etc). > Are you sure you don't have a population of albinos in your region? > > Paul Bradbury > Kamloops, Canada > > ? > kristen arvidson wrote: > >> Help!! Our pathologists are starting to see a new artifact where they >> are unable to see the melanocytes (we are all derm). It only happens >> on a rare occasion. We checked the PH of the water on the stainer >> and it seemed fine. We can't think of anything else? Help! >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amber.mckenzie <@t> gastrodocs.net Wed Jun 11 09:18:34 2008 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Wed Jun 11 09:18:39 2008 Subject: [Histonet] Mohs tech?? In-Reply-To: <003b01c8cb58$b00d2070$360aa8c0@FULLSTAFF.ORG> Message-ID: <03C921A1EAF7F541B16543F6EC6A4B3701BF2633@giamail2.Gia.com> What exactly is a Mohs tech? I'm not familiar with that term? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Tuesday, June 10, 2008 7:19 PM To: 'Histonet' Subject: [Histonet] Need a Mohs temp in NYC Hi All! I need to find a Mohs tech for alternating Mondays starting ASAP (of course :). We could work out a short term situations for a couple of weeks or you could take it on as an ongoing PRN employee. Please call if you have any questions-- Cheryl Cheryl R. Kerry, HT(ASCP), BA Full Staff Inc. Staffing the lab - One GREAT tech at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone admin@fullstaff.org www.fullstaff.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mickie25 <@t> netzero.net Wed Jun 11 09:29:40 2008 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Wed Jun 11 09:30:47 2008 Subject: [Histonet] Mohs tech?? In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B3701BF2633@giamail2.Gia.com> References: <003b01c8cb58$b00d2070$360aa8c0@FULLSTAFF.ORG> <03C921A1EAF7F541B16543F6EC6A4B3701BF2633@giamail2.Gia.com> Message-ID: Dear Amber, A Mohs Tech is a person who cuts Mohs frozen sections for a derm surgeon who does Mohs micrographic skin cancer surgery. If you have any other questions, give me a shout. Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Wednesday, June 11, 2008 7:19 AM To: Histonet Subject: [Histonet] Mohs tech?? What exactly is a Mohs tech? I'm not familiar with that term? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Tuesday, June 10, 2008 7:19 PM To: 'Histonet' Subject: [Histonet] Need a Mohs temp in NYC Hi All! I need to find a Mohs tech for alternating Mondays starting ASAP (of course :). We could work out a short term situations for a couple of weeks or you could take it on as an ongoing PRN employee. Please call if you have any questions-- Cheryl Cheryl R. Kerry, HT(ASCP), BA Full Staff Inc. Staffing the lab - One GREAT tech at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone admin@fullstaff.org www.fullstaff.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Wed Jun 11 09:36:28 2008 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Wed Jun 11 09:36:40 2008 Subject: [Histonet] Granzyme B on BenchmarkXT Message-ID: <484FAAAC.2B7F.00C9.0@geisinger.edu> Would someone mind sharing their protocol for Granzyme B, using Ventana's predilute on the BenchmarkXT? Just looking to save myself some steps. Thanks, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From slappycraw <@t> yahoo.com Wed Jun 11 09:48:22 2008 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Wed Jun 11 09:48:33 2008 Subject: [Histonet] Mohs tech?? In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B3701BF2633@giamail2.Gia.com> Message-ID: <277600.1899.qm@web53605.mail.re2.yahoo.com> I can tell you this: I did Moh's for about 4 years and I probably cut more frozens in that time than I have the rest of my career. We once worked on the same patient from 7am to almost midnight the same day. It can be a lot of cutting and waiting but it is very interesting especially if you get to screen the slides with the dermpath. Amber McKenzie wrote: What exactly is a Mohs tech? I'm not familiar with that term? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Tuesday, June 10, 2008 7:19 PM To: 'Histonet' Subject: [Histonet] Need a Mohs temp in NYC Hi All! I need to find a Mohs tech for alternating Mondays starting ASAP (of course :). We could work out a short term situations for a couple of weeks or you could take it on as an ongoing PRN employee. Please call if you have any questions-- Cheryl Cheryl R. Kerry, HT(ASCP), BA Full Staff Inc. Staffing the lab - One GREAT tech at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone admin@fullstaff.org www.fullstaff.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Larry A. Woody Seattle, Wa. From gu.lang <@t> gmx.at Wed Jun 11 09:52:28 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Jun 11 09:52:35 2008 Subject: AW: [Histonet] Fite stain In-Reply-To: Message-ID: Is it possible, that these spots could be mastcell-granula? It would turn metachromatic red due to methylenblue. - but perhaps I'm totally wrong. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Martin, Erin Gesendet: Mittwoch, 11. Juni 2008 14:57 An: histonet Betreff: [Histonet] Fite stain Good morning everyone, I am having a problem with my Fite stains. Most stain beautifully but I have had trouble with a few cases. Here are the doc's comments: "case xxxx has some lipomembranous change- and this may not really be an entirely false positive result. But there is clearly no explanation for the stuff in xxxx that stains, including some fibrin. I've never seen this before. It's not the usual round spots from inadequately rinsing off the peanut oil...." "A couple of days ago, I saw a granulomatous reaction that had tiny Fite positive "blobbos" that looked like microbes in tiny cystic spaces within the granulomas. I initially thought they might be real organisms but then I did a TB stain and proved that they were nothing. Just moments ago, XXXX had a case that was essentially identical. The Fite stain is working perfectly for ID of microbes, but it seems to have a false positive kind of reactivity that can be seen in small spaces within a granulomatous infiltrate. On an occasional basis only." Does anyone have any idea? Our procedure is 2 changes of xylene/peanut oil, air dry, rinse, carbol fuchsin for 15 min, rinse, differentiate in 1% acid alcohol, rinse, counterstain in methylene blue, air dry. Thanks in advance, _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Wed Jun 11 10:02:50 2008 From: tkngflght <@t> yahoo.com (Cheryl) Date: Wed Jun 11 10:03:01 2008 Subject: What is Mohs RE: [Histonet] Mohs tech?? In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B3701BF2633@giamail2.Gia.com> References: <003b01c8cb58$b00d2070$360aa8c0@FULLSTAFF.ORG> <03C921A1EAF7F541B16543F6EC6A4B3701BF2633@giamail2.Gia.com> Message-ID: <004101c8cbd4$3826a0a0$360aa8c0@FULLSTAFF.ORG> Hi Amber- Mohs is the name of the fellow who pioneered this process. It is basically a skin excisional biopsy with frozens done on the margins to allow the smallest possible tissue removed with clear margins during the initial surgery. There is a color coding system to mark the margins and there are a few other changes in orientation to get the best view of the full margin. The bill codes are only to be used by the doc who is both performing the surgery and reading the slides so it's generally limited to Dermatopathologist practices. Once the excision is done and the margins are cleared on frozen, all the tissue is processed for permanent section. Sometimes the patient is reconstructed right there, other times this part of the process is done by a plastic surgeon. Mohs is usually done on face, head and next skin lesions as they are the most potentially disfiguring places for skin cancers. There are classes for teaching a tech Mohs procedures and if you have a patient Derm doc, they can instruct the orientation, as well. Either way it can be a lot of frozen sections but the pay is commensurate with the pressure of doing frozens with a patient on the table waiting for your results. Hope that helps! Cheryl -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Wednesday, June 11, 2008 9:19 AM To: Histonet Subject: [Histonet] Mohs tech?? What exactly is a Mohs tech? I'm not familiar with that term? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Tuesday, June 10, 2008 7:19 PM To: 'Histonet' Subject: [Histonet] Need a Mohs temp in NYC Hi All! I need to find a Mohs tech for alternating Mondays starting ASAP (of course :). We could work out a short term situations for a couple of weeks or you could take it on as an ongoing PRN employee. Please call if you have any questions-- Cheryl Cheryl R. Kerry, HT(ASCP), BA Full Staff Inc. Staffing the lab - One GREAT tech at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone admin@fullstaff.org www.fullstaff.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vazquezr <@t> ohsu.edu Wed Jun 11 10:06:42 2008 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Wed Jun 11 10:07:12 2008 Subject: [Histonet] Mohs tech?? Message-ID: Amber, A Mohs tech is someone that does frozen sections on dermatological tissue taken off of a patient, from a surgeon. The surgeon brings the tissue to the Mohs tech and they mount, cut, and stain it. Then the surgeon comes and determines if the patient is still positive or negative for skin cancer. I believe I covered the basics? Robyn >>> "Amber McKenzie" 6/11/2008 7:18 AM >>> What exactly is a Mohs tech? I'm not familiar with that term? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Tuesday, June 10, 2008 7:19 PM To: 'Histonet' Subject: [Histonet] Need a Mohs temp in NYC Hi All! I need to find a Mohs tech for alternating Mondays starting ASAP (of course :). We could work out a short term situations for a couple of weeks or you could take it on as an ongoing PRN employee. Please call if you have any questions-- Cheryl Cheryl R. Kerry, HT(ASCP), BA Full Staff Inc. Staffing the lab - One GREAT tech at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone admin@fullstaff.org www.fullstaff.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mohs76009 <@t> yahoo.com Wed Jun 11 10:36:13 2008 From: mohs76009 <@t> yahoo.com (Matt Bancroft) Date: Wed Jun 11 10:36:52 2008 Subject: What is Mohs RE: [Histonet] Mohs tech?? In-Reply-To: <004101c8cbd4$3826a0a0$360aa8c0@FULLSTAFF.ORG> Message-ID: <18743.18933.qm@web63411.mail.re1.yahoo.com> Cheryl,   What you said below is correct, except not all mohs surgens process the for permant sections.  The only time we do it at our facility is if there is a possiable perineural invasion or lymphovascular invasion, or if it is a melanoma and we do what we call slow mohs.   --- On Wed, 6/11/08, Cheryl <tkngflght@yahoo.com> wrote: From: Cheryl <tkngflght@yahoo.com> Subject: What is Mohs RE: [Histonet] Mohs tech?? To: "'Amber McKenzie'" <amber.mckenzie@gastrodocs.net>, "'Histonet'" <histonet@lists.utsouthwestern.edu> Date: Wednesday, June 11, 2008, 10:02 AM Hi Amber- Mohs is the name of the fellow who pioneered this process. It is basically a skin excisional biopsy with frozens done on the margins to allow the smallest possible tissue removed with clear margins during the initial surgery. There is a color coding system to mark the margins and there are a few other changes in orientation to get the best view of the full margin. The bill codes are only to be used by the doc who is both performing the surgery and reading the slides so it's generally limited to Dermatopathologist practices. Once the excision is done and the margins are cleared on frozen, all the tissue is processed for permanent section. Sometimes the patient is reconstructed right there, other times this part of the process is done by a plastic surgeon. Mohs is usually done on face, head and next skin lesions as they are the most potentially disfiguring places for skin cancers. There are classes for teaching a tech Mohs procedures and if you have a patient Derm doc, they can instruct the orientation, as well. Either way it can be a lot of frozen sections but the pay is commensurate with the pressure of doing frozens with a patient on the table waiting for your results. Hope that helps! Cheryl -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Wednesday, June 11, 2008 9:19 AM To: Histonet Subject: [Histonet] Mohs tech?? What exactly is a Mohs tech? I'm not familiar with that term? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Tuesday, June 10, 2008 7:19 PM To: 'Histonet' Subject: [Histonet] Need a Mohs temp in NYC Hi All! I need to find a Mohs tech for alternating Mondays starting ASAP (of course :). We could work out a short term situations for a couple of weeks or you could take it on as an ongoing PRN employee. Please call if you have any questions-- Cheryl Cheryl R. Kerry, HT(ASCP), BA Full Staff Inc. Staffing the lab - One GREAT tech at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone admin@fullstaff.org www.fullstaff.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Wed Jun 11 10:37:40 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Wed Jun 11 10:37:54 2008 Subject: [Histonet] Question for my Canadian colleagues In-Reply-To: <5C0BED61F529364E86309CADEA63FEF20163F41F@TRFT-EX01.xRothGen.nhs.uk> References: <5C0BED61F529364E86309CADEA63FEF20163F41F@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <484FF144.70507@pathology.washington.edu> Terry, The number of autopsies seems high, do all deceased get an autopsy? Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Marshall Terry Dr, Consultant Histopathologist wrote: > No. of pathologists/residents: 3 > No. of techs: 3 > No. of Assistants: 2 > No. of surgicals done annually (round to nearest 1000): 16000 > No. of autopsies: 740 > > Terry > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Greg > Dobbin > Sent: 10 June 2008 18:37 > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Question for my Canadian colleagues > > Hi folks, > I am conducting an informal survey to find out how other labs are > staffed (believing mine to be understaffed). > Could you please provide me with the following numbers: > > No. of pathologists/residents: 3 > No. of techs: 3 > No. of Assistants: 2 > No. of surgicals done annually (round to nearest 1000): 16000 > No. of autopsies: 740 > > Thanks in advance for any assistance you may be able to offer in this > regard. > Sincerely, > Greg > > Greg Dobbin, R.T. > Chief Technologist, Histology Lab > Dept. of Laboratory Medicine, > Queen Elizabeth Hospital, > P.O. Box 6600 > Charlottetown, PE C1A 8T5 > Phone: (902) 894-2337 > Fax: (902) 894-2385 > > There is some merit in doing the right thing rather badly, but > absolutely none in doing the wrong thing excellently! > > ------------------------- > Statement of Confidentiality > This message (including attachments) may contain confidential or > privileged information intended for a specific individual or > organization. If you have received this communication in error, please > notify the sender immediately. If you are not the intended recipient, > you are not authorized to use, disclose, distribute, copy, print or rely > on this email, and should promptly delete this email from your entire > computer system. > > D?claration de confidentialit? > Le pr?sent message (y compris les annexes) peut contenir des > renseignements confidentiels ? l'intention d'une personne ou d'un > organisme particulier. Si vous avez re?u la pr?sente communication par > erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes > pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, > divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous > en servir, et vous devriez l'effacer imm?diatement de votre syst?me > informatique. > ------------------------- > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tkngflght <@t> yahoo.com Wed Jun 11 10:48:11 2008 From: tkngflght <@t> yahoo.com (Cheryl) Date: Wed Jun 11 10:48:27 2008 Subject: What is Mohs RE: [Histonet] Mohs tech?? In-Reply-To: <18743.18933.qm@web63411.mail.re1.yahoo.com> References: <004101c8cbd4$3826a0a0$360aa8c0@FULLSTAFF.ORG> <18743.18933.qm@web63411.mail.re1.yahoo.com> Message-ID: <006701c8cbda$8e2927b0$360aa8c0@FULLSTAFF.ORG> Hi Matt- I truly didn't know that not all derms processed the remaining tissue for permanent. All the Mohs docs I've worked with sent the remaining tissue out to a partner or reference lab and correlated the results. It generates additional pathology fees, of course...why would they not want their work backed up by permanents?? Cheryl _____ From: Matt Bancroft [mailto:mohs76009@yahoo.com] Sent: Wednesday, June 11, 2008 10:36 AM To: 'Amber McKenzie'; 'Histonet'; tkngflght@yahoo.com Subject: Re: What is Mohs RE: [Histonet] Mohs tech?? Cheryl, What you said below is correct, except not all mohs surgens process the for permant sections. The only time we do it at our facility is if there is a possiable perineural invasion or lymphovascular invasion, or if it is a melanoma and we do what we call slow mohs. --- On Wed, 6/11/08, Cheryl wrote: From: Cheryl Subject: What is Mohs RE: [Histonet] Mohs tech?? To: "'Amber McKenzie'" , "'Histonet'" Date: Wednesday, June 11, 2008, 10:02 AM Hi Amber- Mohs is the name of the fellow who pioneered this process. It is basically a skin excisional biopsy with frozens done on the margins to allow the smallest possible tissue removed with clear margins during the initial surgery. There is a color coding system to mark the margins and there are a few other changes in orientation to get the best view of the full margin. The bill codes are only to be used by the doc who is both performing the surgery and reading the slides so it's generally limited to Dermatopathologist practices. Once the excision is done and the margins are cleared on frozen, all the tissue is processed for permanent section. Sometimes the patient is reconstructed right there, other times this part of the process is done by a plastic surgeon. Mohs is usually done on face, head and next skin lesions as they are the most potentially disfiguring places for skin cancers. There are classes for teaching a tech Mohs procedures and if you have a patient Derm doc, they can instruct the orientation, as well. Either way it can be a lot of frozen sections but the pay is commensurate with the pressure of doing frozens with a patient on the table waiting for your results. Hope that helps! Cheryl -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Wednesday, June 11, 2008 9:19 AM To: Histonet Subject: [Histonet] Mohs tech?? What exactly is a Mohs tech? I'm not familiar with that term? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Tuesday, June 10, 2008 7:19 PM To: 'Histonet' Subject: [Histonet] Need a Mohs temp in NYC Hi All! I need to find a Mohs tech for alternating Mondays starting ASAP (of course :). We could work out a short term situations for a couple of weeks or you could take it on as an ongoing PRN employee. Please call if you have any questions-- Cheryl Cheryl R. Kerry, HT(ASCP), BA Full Staff Inc. Staffing the lab - One GREAT tech at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone admin@fullstaff.org www.fullstaff.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kbowden <@t> ucsd.edu Wed Jun 11 10:48:17 2008 From: kbowden <@t> ucsd.edu (kbowden) Date: Wed Jun 11 10:48:29 2008 Subject: [Histonet] Mohs tech?? In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B3701BF2633@giamail2.Gia.com> References: <03C921A1EAF7F541B16543F6EC6A4B3701BF2633@giamail2.Gia.com> Message-ID: <484FF3C1.5050406@ucsd.edu> If you go to this web site it will give you great details on Mohs. Right down to the fact that the procedure is names after Dr. Fredrick E. Mohs. Karen Amber McKenzie wrote: > What exactly is a Mohs tech? I'm not familiar with that term? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl > Sent: Tuesday, June 10, 2008 7:19 PM > To: 'Histonet' > Subject: [Histonet] Need a Mohs temp in NYC > > Hi All! > > I need to find a Mohs tech for alternating Mondays starting ASAP (of > course > :). We could work out a short term situations for a couple of weeks or > you > could take it on as an ongoing PRN employee. > > Please call if you have any questions-- > > Cheryl > > > Cheryl R. Kerry, HT(ASCP), BA > > Full Staff Inc. > > Staffing the lab - One GREAT tech at a time. > > 281.852.9457 office > > 281.883.7704 cell > > 800.756.3309 fax and alternate phone > > admin@fullstaff.org > > www.fullstaff.org > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From kbowden <@t> ucsd.edu Wed Jun 11 10:50:33 2008 From: kbowden <@t> ucsd.edu (kbowden) Date: Wed Jun 11 10:50:43 2008 Subject: [Histonet] Mohs tech?? In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B3701BF2633@giamail2.Gia.com> References: <03C921A1EAF7F541B16543F6EC6A4B3701BF2633@giamail2.Gia.com> Message-ID: <484FF449.8050804@ucsd.edu> Ooops, I always forget to include the site or attachment. Sorry If you go to this web site it will give you great details on Mohs. Right down to the fact that the procedure is names after Dr. Fredrick E. Mohs. http://en.wikipedia.org/wiki/Mohs_cancer_surgery Karen Amber McKenzie wrote: > What exactly is a Mohs tech? I'm not familiar with that term? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl > Sent: Tuesday, June 10, 2008 7:19 PM > To: 'Histonet' > Subject: [Histonet] Need a Mohs temp in NYC > > Hi All! > > I need to find a Mohs tech for alternating Mondays starting ASAP (of > course > :). We could work out a short term situations for a couple of weeks or > you > could take it on as an ongoing PRN employee. > > Please call if you have any questions-- > > Cheryl > > > Cheryl R. Kerry, HT(ASCP), BA > > Full Staff Inc. > > Staffing the lab - One GREAT tech at a time. > > 281.852.9457 office > > 281.883.7704 cell > > 800.756.3309 fax and alternate phone > > admin@fullstaff.org > > www.fullstaff.org > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From september.amspacher <@t> bassett.org Wed Jun 11 10:49:32 2008 From: september.amspacher <@t> bassett.org (Amspacher, September) Date: Wed Jun 11 10:52:21 2008 Subject: [Histonet] Hematoxylin shortage Message-ID: <85C561E8-BBEE-4F82-B8B9-DE1680541281@mimectl> Hello all, I know that this has been hot topic as of late and all was good in my lab until yesterday when I was told that Richard Allen was no longer shipping hematoxylin. Phoenix blue (sp?) is now being shipped as the substitute. I was wondering if anyone is already using this product and if they have any thoughts or helpful hints about it. Thanks a bunch- grateful for any help! September Amspacher HT(ASCP) Sr. Tech- Histology Department Bassett Healthcare, Cooperstown New York NOTICE OF CONFIDENTIALITY This electronic message, including attachments, is for the sole use of the named recipient and may contain confidential or privileged information protected by New York State, and Federal regulations. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. If you are not the intended recipient or have received this communication in error please contact the sender or email.security@bassett.org and destroy all copies of the original message. Thank you. From PMonfils <@t> Lifespan.org Wed Jun 11 11:30:32 2008 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Jun 11 11:30:41 2008 Subject: [Histonet] staining of cell membranes in sections Message-ID: <4EBFF65383B74D49995298C4976D1D5E273D93@LSRIEXCH1.lsmaster.lifespan.org> Does anyone know of a procedure for staining cell membranes (also called cell boundaries) in tissue sections so that the individual cells are clearly outlined when viewed with the light microscope? I knew of a couple such techniques many years ago but cannot find much on the subject currently. From aep10 <@t> cornell.edu Wed Jun 11 12:02:58 2008 From: aep10 <@t> cornell.edu (Anna Elisse Beaudin) Date: Wed Jun 11 12:03:01 2008 Subject: [Histonet] very specific background immunofluorescence in mouse embryo sections Message-ID: <3389.128.253.96.191.1213203778.squirrel@webmail.cornell.edu> Hello, We've been doing some immunofluorescence in mouse embryos sections (E9-10), and have recently encountered a problem involving very specific immunofluorescent 'staining' in our negative controls. the staining looks to be in specific cells in specific regions, and occurs in both our no-primary and no-secondary controls. we are staining with a rabbit anti-caspase, followed by an alexafluor-conjugated donkey anti-rabbit. the background 'staining' if very bright and can be seen with several different filters. has anyone else ever experienced anything like this? we are stumped. I have never had this kind of problem previously in other mouse tissues. if anyone has any insight, I would greatly appreciate your input. Thanks in advance, Anna Beaudin Division of Nutritional Sciences Cornell University From John.Spair <@t> multicare.org Wed Jun 11 12:28:44 2008 From: John.Spair <@t> multicare.org (John Spair) Date: Wed Jun 11 12:35:57 2008 Subject: [Histonet] RE: Histonet Digest, Vol 55, Issue 19 In-Reply-To: <644ED7AB1RC93014-01@MMS_multicare.org> References: <644ED7AB1RC93014-01@MMS_multicare.org> Message-ID: <61A9977919846C479389493BAE2517CA023356CA@MHSEXMBX1.multicare.org> This one caught my eye for sure. I use Richard Allen hematoxylin for routine and frozen sections and I haven't been having a problem getting it nor have I heard anything about it not being available or being substituted with something else. I never even heard of Phoenix blue and it would require lots of testing on my end before I'd even consider switching. So what's the story, is any of this true?? From: "Amspacher, September" Subject: [Histonet] Hematoxylin shortage To: "histonet@lists.utsouthwestern.edu" Message-ID: <85C561E8-BBEE-4F82-B8B9-DE1680541281@mimectl> Content-Type: text/plain; charset="iso-8859-1" Hello all, I know that this has been hot topic as of late and all was good in my lab until yesterday when I was told that Richard Allen was no longer shipping hematoxylin. Phoenix blue (sp?) is now being shipped as the substitute. I was wondering if anyone is already using this product and if they have any thoughts or helpful hints about it. Thanks a bunch- grateful for any help! September Amspacher HT(ASCP) Sr. Tech- Histology Department Bassett Healthcare, Cooperstown New York "MMS " made the following annotations. ------------------------------------------------------------------------------ NOTICE: This e-mail and the attachments hereto, if any, may contain privileged and/or confidential information. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, you are hereby notified that any examination, distribution or copying of this e-mail and the attachments hereto, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by email or telephone and permanently delete this e-mail and the attachments hereto, if any, and destroy any printout thereof. MultiCare Health System, Tacoma, WA 98415 (253) 403-1000. ============================================================================== From rjbuesa <@t> yahoo.com Wed Jun 11 12:38:18 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 11 12:38:22 2008 Subject: [Histonet] staining of cell membranes in sections In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E273D93@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <239820.62729.qm@web65711.mail.ac4.yahoo.com> Leber (1860) recommends using a 1% ferric chloride solution for 5 minutes, followed by a rinse and a 5% potassium ferrocyanide solution for 2-3 minutes for the delineation of cells outlines, that I think could work for what you need. Ren? J. "Monfils, Paul" wrote: Does anyone know of a procedure for staining cell membranes (also called cell boundaries) in tissue sections so that the individual cells are clearly outlined when viewed with the light microscope? I knew of a couple such techniques many years ago but cannot find much on the subject currently. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Wed Jun 11 12:45:34 2008 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Wed Jun 11 12:45:53 2008 Subject: [Histonet] very specific background immunofluorescence in mouse embryo sections In-Reply-To: <3389.128.253.96.191.1213203778.squirrel@webmail.cornell.edu> References: <3389.128.253.96.191.1213203778.squirrel@webmail.cornell.edu> Message-ID: Do you have photos of the background? Glad to help with some more info ... Where is the background occuring, what organs and what cells are fluorescent? Could it be RBCs? How are you preparing the embryos prior to staining? Fixation, paraffin, frozen, post-fixation, blocking etc etc ... Also are you using an particular type of pen or pencil on your slides which could be rubbing down into your sections? What agents are you using to coverslip? Do you use any sealant around your coverslips? Andrea At 1:02 PM -0400 6/11/08, Anna Elisse Beaudin wrote: >Hello, > > We've been doing some immunofluorescence in mouse embryos sections >(E9-10), and have recently encountered a problem involving very specific >immunofluorescent 'staining' in our negative controls. the staining >looks to be in specific cells in specific regions, and occurs in both >our no-primary and no-secondary controls. we are staining with a >rabbit anti-caspase, followed by an alexafluor-conjugated donkey >anti-rabbit. the background 'staining' if very bright and can be seen >with several different filters. has anyone else ever experienced >anything like this? we are stumped. I have never had this kind of >problem previously in other mouse tissues. if anyone has any insight, I >would greatly appreciate your input. > >Thanks in advance, > >Anna Beaudin >Division of Nutritional Sciences >Cornell University > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- From september.amspacher <@t> bassett.org Wed Jun 11 12:45:04 2008 From: september.amspacher <@t> bassett.org (Amspacher, September) Date: Wed Jun 11 12:49:07 2008 Subject: [Histonet] RE: Histonet Digest, Vol 55, Issue 19 In-Reply-To: <61A9977919846C479389493BAE2517CA023356CA@MHSEXMBX1.multicare.org> References: <644ED7AB1RC93014-01@MMS_multicare.org>, <61A9977919846C479389493BAE2517CA023356CA@MHSEXMBX1.multicare.org> Message-ID: We originally used the Shandon instant hematoxylin and then when we were told that it would be backordered until at least fall, if not later we ordered a case (4 bottles) of hematoxylin 7211 the signature series hematoxylin from Richard Allen, I went though the validation process for it and then went to order it and was told the phoenix blue was our option now. When I called Richard Allen about it, I was told that hematoxylin would be backordered and the shortage would be until- they were thinking late fall. -September Amspacher HT(ASCP) Sr. Tech- Histology Department Bassett Healthcare, Cooperstown New York From: John Spair Sent: Wed 6/11/2008 1:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 55, Issue 19 This one caught my eye for sure. I use Richard Allen hematoxylin for routine and frozen sections and I haven't been having a problem getting it nor have I heard anything about it not being available or being substituted with something else. I never even heard of Phoenix blue and it would require lots of testing on my end before I'd even consider switching. So what's the story, is any of this true?? From: "Amspacher, September" Subject: [Histonet] Hematoxylin shortage To: "histonet@lists.utsouthwestern.edu" Message-ID: <85C561E8-BBEE-4F82-B8B9-DE1680541281@mimectl> Content-Type: text/plain; charset="iso-8859-1" Hello all, I know that this has been hot topic as of late and all was good in my lab until yesterday when I was told that Richard Allen was no longer shipping hematoxylin. Phoenix blue (sp?) is now being shipped as the substitute. I was wondering if anyone is already using this product and if they have any thoughts or helpful hints about it. Thanks a bunch- grateful for any help! September Amspacher HT(ASCP) Sr. Tech- Histology Department Bassett Healthcare, Cooperstown New York "MMS " made the following annotations. ------------------------------------------------------------------------------ NOTICE: This e-mail and the attachments hereto, if any, may contain privileged and/or confidential information. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, you are hereby notified that any examination, distribution or copying of this e-mail and the attachments hereto, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by email or telephone and permanently delete this e-mail and the attachments hereto, if any, and destroy any printout thereof. MultiCare Health System, Tacoma, WA 98415 (253) 403-1000. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE OF CONFIDENTIALITY This electronic message, including attachments, is for the sole use of the named recipient and may contain confidential or privileged information protected by New York State, and Federal regulations. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. If you are not the intended recipient or have received this communication in error please contact the sender or email.security@bassett.org and destroy all copies of the original message. Thank you. From dayers1 <@t> jhmi.edu Wed Jun 11 12:50:11 2008 From: dayers1 <@t> jhmi.edu (Deborah Duckworth) Date: Wed Jun 11 12:50:24 2008 Subject: [Histonet] Glass coverslipper mounting media Message-ID: <484FD8130200005100023324@cis27.hosts.jhmi.edu> We recently purchased 3 glass coverslippers and are having a heck of a time with slides that are stuck together. They were marketed as "24 hour dry time" and not one week dry time! Funny how that works. So with over two thousand slides per day, we can't not file the material. Ovens work but who has the space. Are there any other mounting medias (non-toluene based) that have faster dry times? ANY suggestions at all would be great. Thanks, Deborah From kenneth.metzger <@t> aruplab.com Wed Jun 11 12:54:34 2008 From: kenneth.metzger <@t> aruplab.com (Metzger, Kenneth) Date: Wed Jun 11 12:54:03 2008 Subject: [Histonet] Storage of Whole Mount Slides Message-ID: Does anyone know where to get storage drawers or boxes for 3 inch x 2 inch whole mount slides? Thanks, Ken Ken Metzger HTL(ASCP) Histology Supervisor ARUP Laboratories 500 Chipeta way Salt Lake City, UT 84108 801.583.2787 ext 3101 - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From mohs76009 <@t> yahoo.com Wed Jun 11 13:10:46 2008 From: mohs76009 <@t> yahoo.com (Matt Bancroft) Date: Wed Jun 11 13:10:51 2008 Subject: What is Mohs RE: [Histonet] Mohs tech?? In-Reply-To: <006701c8cbda$8e2927b0$360aa8c0@FULLSTAFF.ORG> Message-ID: <445392.59858.qm@web63403.mail.re1.yahoo.com> If you have a good tech and have good cryo-sections, you don't need to do that.  That is why the physicans go to school so they learn how to read the slides --- On Wed, 6/11/08, Cheryl <tkngflght@yahoo.com> wrote: From: Cheryl <tkngflght@yahoo.com> Subject: RE: What is Mohs RE: [Histonet] Mohs tech?? To: mohs76009@yahoo.com, "'Histonet'" <histonet@lists.utsouthwestern.edu> Date: Wednesday, June 11, 2008, 10:48 AM Hi Matt-   I truly didn't know that not all derms processed the remaining tissue for permanent.  All the Mohs docs I've worked with sent the remaining tissue out to a partner or reference lab and correlated the results.  It generates additional pathology fees, of course...why would they not want their work backed up by permanents??   Cheryl     From: Matt Bancroft [mailto:mohs76009@yahoo.com] Sent: Wednesday, June 11, 2008 10:36 AM To: 'Amber McKenzie'; 'Histonet'; tkngflght@yahoo.com Subject: Re: What is Mohs RE: [Histonet] Mohs tech?? Cheryl,   What you said below is correct, except not all mohs surgens process the for permant sections.  The only time we do it at our facility is if there is a possiable perineural invasion or lymphovascular invasion, or if it is a melanoma and we do what we call slow mohs.   --- On Wed, 6/11/08, Cheryl <tkngflght@yahoo.com> wrote: From: Cheryl <tkngflght@yahoo.com> Subject: What is Mohs RE: [Histonet] Mohs tech?? To: "'Amber McKenzie'" <amber.mckenzie@gastrodocs.net>, "'Histonet'" <histonet@lists.utsouthwestern.edu> Date: Wednesday, June 11, 2008, 10:02 AM Hi Amber- Mohs is the name of the fellow who pioneered this process. It is basically a skin excisional biopsy with frozens done on the margins to allow the smallest possible tissue removed with clear margins during the initial surgery. There is a color coding system to mark the margins and there are a few other changes in orientation to get the best view of the full margin. The bill codes are only to be used by the doc who is both performing the surgery and reading the slides so it's generally limited to Dermatopathologist practices. Once the excision is done and the margins are cleared on frozen, all the tissue is processed for permanent section. Sometimes the patient is reconstructed right there, other times this part of the process is done by a plastic surgeon. Mohs is usually done on face, head and next skin lesions as they are the most potentially disfiguring places for skin cancers. There are classes for teaching a tech Mohs procedures and if you have a patient Derm doc, they can instruct the orientation, as well. Either way it can be a lot of frozen sections but the pay is commensurate with the pressure of doing frozens with a patient on the table waiting for your results. Hope that helps! Cheryl -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Wednesday, June 11, 2008 9:19 AM To: Histonet Subject: [Histonet] Mohs tech?? What exactly is a Mohs tech? I'm not familiar with that term? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Tuesday, June 10, 2008 7:19 PM To: 'Histonet' Subject: [Histonet] Need a Mohs temp in NYC Hi All! I need to find a Mohs tech for alternating Mondays starting ASAP (of course :). We could work out a short term situations for a couple of weeks or you could take it on as an ongoing PRN employee. Please call if you have any questions-- Cheryl Cheryl R. Kerry, HT(ASCP), BA Full Staff Inc. Staffing the lab - One GREAT tech at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone admin@fullstaff.org www.fullstaff.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maxim_71 <@t> mail.ru Wed Jun 11 13:09:24 2008 From: Maxim_71 <@t> mail.ru (Maxim_71@mail.ru) Date: Wed Jun 11 13:12:34 2008 Subject: : [Histonet] Fite stain Message-ID: <1663481537.20080611220924@mail.ru> Try stain another slides by Gram. As TB as these organisms must be slightly Gram-positive. Maxim Peshkov Russia, Taganrog. mailto:Maxim_71@mail.ru From Maxim_71 <@t> mail.ru Wed Jun 11 13:09:24 2008 From: Maxim_71 <@t> mail.ru (Maxim_71@mail.ru) Date: Wed Jun 11 13:13:22 2008 Subject: : [Histonet] Fite stain Message-ID: <1663481537.20080611220924@mail.ru> Try stain another slides by Gram. As TB as these organisms must be slightly Gram-positive. Maxim Peshkov Russia, Taganrog. mailto:Maxim_71@mail.ru From freckles9660 <@t> yahoo.com Wed Jun 11 13:49:58 2008 From: freckles9660 <@t> yahoo.com (Karla Arrington) Date: Wed Jun 11 13:50:02 2008 Subject: [Histonet] Which one? Message-ID: <650440.1745.qm@web32502.mail.mud.yahoo.com> Fellow Histo's... For the traveling HT's out there. Which traveling agency is a good one or reputable? Club Staffing, Resources One or others? The good, bad and ugly wanted. freckles9660@yahoo.com From ROrr <@t> enh.org Wed Jun 11 14:08:51 2008 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Wed Jun 11 14:08:55 2008 Subject: [Histonet] Granzyme B on BMK Message-ID: Hi Angie I run this product on the Benchmark with the UltraView kit Mild CC1 Antibody incubates 16 minutes. The results are so beautiful I could just cry with joy. Becky Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 From asachau <@t> titanmed.com Wed Jun 11 14:38:08 2008 From: asachau <@t> titanmed.com (April Sachau) Date: Wed Jun 11 14:41:41 2008 Subject: [Histonet] Histology Job in the Midwest! In-Reply-To: Message-ID: <7E3ACD48BA6E26408F3188FBF08693F70151D24A@titansbs1.corp.titanmed.com> Hello Histonetters! I have a GREAT histology position available in the Midwest! It is a 9 month assignment. Routine Histology, days Monday through Friday. Housing and travel provided. GREAT compensation package!!! ASCP is required. Please contact me if you are interested! April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com From Pat.Bell <@t> UCHSC.edu Wed Jun 11 15:03:05 2008 From: Pat.Bell <@t> UCHSC.edu (Pat.Bell@UCHSC.edu) Date: Wed Jun 11 15:03:09 2008 Subject: [Histonet] Storage of Whole Mount Slides In-Reply-To: Message-ID: <71FCC52823941D49A4BC39B48C6E3428F3C02F@java.uchsc.edu> Try VWR. I used to get slides boxes that size from them. Pat Bell HT(ASCP) Sr. PRA Division of Medical Oncology UCHSC, Aurora, CO 303-724-3845 (lab) 303-724-3889 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Metzger, Kenneth Sent: Wednesday, June 11, 2008 11:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Storage of Whole Mount Slides Does anyone know where to get storage drawers or boxes for 3 inch x 2 inch whole mount slides? Thanks, Ken Ken Metzger HTL(ASCP) Histology Supervisor ARUP Laboratories 500 Chipeta way Salt Lake City, UT 84108 801.583.2787 ext 3101 - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Wed Jun 11 15:57:21 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Jun 11 15:57:31 2008 Subject: [Histonet] HPV question Message-ID: <485003F102000077000035E8@gwmail6.harthosp.org> I have an ENT surgeon who wants to differentiate between HPV6 and HPV11 in a laryngeal squamous papilloma for prognosis. I am familiar with DNA probes to HPV6/11, but not with the individual types. Is anyone doing this? Thanks. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From Traczyk7 <@t> aol.com Wed Jun 11 15:58:53 2008 From: Traczyk7 <@t> aol.com (Traczyk7@aol.com) Date: Wed Jun 11 15:59:08 2008 Subject: [Histonet] Storage of Whole Mount Slides (Vendor reply) Message-ID: Ken, I can get you just what you need. It's a seven drawer cabinet with spring insert to keep individual 3x2 slides upright and separated. Nice thing about this unit is that you can combine traditional slide and block storage in the other drawers. The cabinets are also stackable and will take up a minimum of floor space. I will send a quote under separate cover. Dorothy Hacker Instruments Winnsboro, SC In a message dated 6/11/2008 1:54:36 P.M. Eastern Daylight Time, kenneth.metzger@aruplab.com writes: Does anyone know where to get storage drawers or boxes for 3 inch x 2 inch whole mount slides? Thanks, Ken Ken Metzger HTL(ASCP) Histology Supervisor ARUP Laboratories 500 Chipeta way Salt Lake City, UT 84108 801.583.2787 ext 3101 - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************Vote for your city's best dining and nightlife. City's Best 2008. (http://citysbest.aol.com?ncid=aolacg00050000000102) From amosbrooks <@t> gmail.com Wed Jun 11 17:18:19 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Jun 11 17:18:28 2008 Subject: [Histonet] Slide Printer Message-ID: <582736990806111518j7cab0caapb1f2ba35c16a8201@mail.gmail.com> Rosa, Good labeler concept, but it is a victim of a really bad proprietary issue. It is unfortunate that the only company that I have found that makes Colormark slides (or anything like them) is Erie Scientific. (Suggestions anyone?) They have terrible quality control. Tissue is constantly falling off our slides (yes, the expensive charged ones) there have been scratches down the center of many of them. We are now using adhesive in the float baths in spite of using the charged slides. I would rather deal with background staining than tissue falling off. Thermo has offered to replace bad slides, but who will replace the time it takes to cut them not to mention the wasted tissues. If I had known I would be going through this with the slides I would not have bought the labeler. I wish you the best with this, honestly, Amos Brooks Message: 5 Date: Tue, 10 Jun 2008 12:57:31 -0500 From: "Rosa Fields" Subject: RE: [Histonet] Slide printer To: "Clark Stephen - Myrtle Beach" , Message-ID: <2F2611250DCD6549AA3D96CE8AF1F01801124072@giexchange.gidocs.net> Content-Type: text/plain; charset="iso-8859-1" We have just gotten a slide etcher from Thermo, manufactured by RA Lamb, very simple to use, no ink! Small footprint to boot (no pun intended!) Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net From AnthonyH <@t> chw.edu.au Wed Jun 11 18:19:39 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Jun 11 18:19:55 2008 Subject: [Histonet] Fite stain In-Reply-To: Message-ID: Your differentiation step is used in the classic ZN not a Fite. This might just be a typo but shouldn't it be 1-3% sulphuric acid (or other acid). The bugs you are trying to demonstrate with the Fite stain are acid fast but acid-alcohol labile. This won't solve the problem with you odd staining though Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Erin Sent: Wednesday, 11 June 2008 10:57 PM To: histonet Subject: [Histonet] Fite stain Good morning everyone, I am having a problem with my Fite stains. Most stain beautifully but I have had trouble with a few cases. Here are the doc's comments: "case xxxx has some lipomembranous change- and this may not really be an entirely false positive result. But there is clearly no explanation for the stuff in xxxx that stains, including some fibrin. I've never seen this before. It's not the usual round spots from inadequately rinsing off the peanut oil...." "A couple of days ago, I saw a granulomatous reaction that had tiny Fite positive "blobbos" that looked like microbes in tiny cystic spaces within the granulomas. I initially thought they might be real organisms but then I did a TB stain and proved that they were nothing. Just moments ago, XXXX had a case that was essentially identical. The Fite stain is working perfectly for ID of microbes, but it seems to have a false positive kind of reactivity that can be seen in small spaces within a granulomatous infiltrate. On an occasional basis only." Does anyone have any idea? Our procedure is 2 changes of xylene/peanut oil, air dry, rinse, carbol fuchsin for 15 min, rinse, differentiate in 1% acid alcohol, rinse, counterstain in methylene blue, air dry. Thanks in advance, _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From renafail <@t> bellsouth.net Wed Jun 11 18:41:40 2008 From: renafail <@t> bellsouth.net (Rena Fail) Date: Wed Jun 11 18:41:41 2008 Subject: [Histonet] Fite stain In-Reply-To: Message-ID: <000001c8e478$8f543c90$0301a8c0@RENAD4YK9B8ABE> Acid alcohol will remove your dye from those bugs requiring the peanut oil (usually Lepra Bacilli). It will also remove it from other acid fast organisms, but at a slower rate. 1% sulfuric acid differentiation gives more consistent results. Rena Fail -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Wednesday, June 11, 2008 7:20 PM To: Martin, Erin; histonet Subject: RE: [Histonet] Fite stain Your differentiation step is used in the classic ZN not a Fite. This might just be a typo but shouldn't it be 1-3% sulphuric acid (or other acid). The bugs you are trying to demonstrate with the Fite stain are acid fast but acid-alcohol labile. This won't solve the problem with you odd staining though Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Erin Sent: Wednesday, 11 June 2008 10:57 PM To: histonet Subject: [Histonet] Fite stain Good morning everyone, I am having a problem with my Fite stains. Most stain beautifully but I have had trouble with a few cases. Here are the doc's comments: "case xxxx has some lipomembranous change- and this may not really be an entirely false positive result. But there is clearly no explanation for the stuff in xxxx that stains, including some fibrin. I've never seen this before. It's not the usual round spots from inadequately rinsing off the peanut oil...." "A couple of days ago, I saw a granulomatous reaction that had tiny Fite positive "blobbos" that looked like microbes in tiny cystic spaces within the granulomas. I initially thought they might be real organisms but then I did a TB stain and proved that they were nothing. Just moments ago, XXXX had a case that was essentially identical. The Fite stain is working perfectly for ID of microbes, but it seems to have a false positive kind of reactivity that can be seen in small spaces within a granulomatous infiltrate. On an occasional basis only." Does anyone have any idea? Our procedure is 2 changes of xylene/peanut oil, air dry, rinse, carbol fuchsin for 15 min, rinse, differentiate in 1% acid alcohol, rinse, counterstain in methylene blue, air dry. Thanks in advance, _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From llewllew <@t> shaw.ca Wed Jun 11 18:43:24 2008 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Wed Jun 11 18:44:02 2008 Subject: [Histonet] Fite stain References: Message-ID: <002201c8cc1c$f0626530$0a514246@yourlk4rlmsu> Sometimes an old carbol fuchsin solution will leave tiny droplets on the slide. This may be the source of the problem. Make up fresh carbol fuchsin, or buy it, and try again. If you are doing the Fite stain for TB organisms and not for Leprosy in particular, why not consider switching to a regular ZN. Bryan Llewellyn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Erin Sent: Wednesday, 11 June 2008 10:57 PM To: histonet Subject: [Histonet] Fite stain Good morning everyone, I am having a problem with my Fite stains. Most stain beautifully but I have had trouble with a few cases. Here are the doc's comments: "case xxxx has some lipomembranous change- and this may not really be an entirely false positive result. But there is clearly no explanation for the stuff in xxxx that stains, including some fibrin. I've never seen this before. It's not the usual round spots from inadequately rinsing off the peanut oil...." "A couple of days ago, I saw a granulomatous reaction that had tiny Fite positive "blobbos" that looked like microbes in tiny cystic spaces within the granulomas. I initially thought they might be real organisms but then I did a TB stain and proved that they were nothing. Just moments ago, XXXX had a case that was essentially identical. The Fite stain is working perfectly for ID of microbes, but it seems to have a false positive kind of reactivity that can be seen in small spaces within a granulomatous infiltrate. On an occasional basis only." Does anyone have any idea? Our procedure is 2 changes of xylene/peanut oil, air dry, rinse, carbol fuchsin for 15 min, rinse, differentiate in 1% acid alcohol, rinse, counterstain in methylene blue, air dry. Thanks in advance, _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kjnpeppa <@t> yahoo.com Wed Jun 11 19:40:36 2008 From: kjnpeppa <@t> yahoo.com (Barbara Rouse) Date: Wed Jun 11 19:40:44 2008 Subject: [Histonet] Logging in same type specimens consecutively Message-ID: <197710.53582.qm@web53705.mail.re2.yahoo.com> Does anyone know of a regulation by CAP that states that same type specimens should not be logged in consecutively? Thanks From histology.bc <@t> shaw.ca Wed Jun 11 20:40:24 2008 From: histology.bc <@t> shaw.ca (Histonet) Date: Wed Jun 11 20:41:59 2008 Subject: [Histonet] Training assistants to work in Histology Message-ID: <48507E88.1040002@shaw.ca> A couple of days ago, there was a question about training assistants to work in a Histology laboratory. I have been involved in this topic for some years now and can offer some comments. Here in Canada, Medical Laboratory Assistants have been used as a vital part of the laboratory workforce for many years. The national certifying body, the Canadian Society for Medical Laboratory Sciences, has adopted the MLA group and has established a National Syllabus of Studies and required competencies for each laboratory discipline. By standardizing the required competencies and the training of MLA's, it is possible to clearly define the precise role and duties that an MLA is permitted to perform. The duties that MLA's perform have been specifically chosen to avoid any erosion of responsibilities from the technologist's duties. In practice, certified technologists can perform any and all duties in the laboratory, whereas MLA's may perform only specific tasks. MLA's are paid on a lower scale than a certified technologist to reflect their more limited responsibilities. The separation of technologist's and assistant's duties is vital, especially in a situation where employers may be tempted to employ the less expensive of the two groups in order to cut their costs. Medical Laboratory Assistants are permitted to work in the gross room, accessioning specimens, entering data into the computer, labeling cassettes, filing and retrieving specimens, filing and retrieving blocks. The assistants also maintain the tissue processors and fluid levels. However, gross descriptions, specimen selection and dissection are the sole responsibility of the technologists. Embedding, sectioning, frozen sections, and staining are also the responsibility of the technologists. By assigning the more mundane, but still critical, tasks to less qualified personnel, the technologists are available to concentrate on the more demanding procedures. This system has worked very well and very effectively for many years. There is a distance education course for Histology Assistants offered through the Open Learning Division of Thompson Rivers University of British Columbia. This is specifically written for workers who have no prior knowledge of histology. It focuses on "pre-analytical procedures" but also provides a good deal of background information on diagnostic histopathology, tissues, fixation, tissues types, common specimens, tissue processing, filing, storage. It also covers some aspects of specimen preparation for cytology specimens, fluids, aspirates, etc. http://www.tru.ca/distance/programs/health_sci/medlabasst/courses.html#mlap161 The Open Learning course is available to anyone, anywhere, and may be completed within a very flexible time frame. The current cost of the course is $400.00, including all course materials, examinations, on-line tutors, and toll-free tutor phone calls. Paul Bradbury, Kamloops, Canada ************************************************************************************************************* McKnight, Tanisha wrote: Hello All: I have a few people, now working in accessioning, who are interested in working in Histology. I am thinking of potentially creating "Histology Lab Assistant" positions to help them transition. They have already been told that they will need to go through an accredited program to become full Techs. We have one here in Indiana that I went through and it is great. Can you all share your strategies for training? How do you separate what Assistants are allowed to do from what Techs do? What regulatory guidelines do you follow if any when deciding? I was thinking of training them to embed and create sections first (on limited specimen types). I would not allow certain biopsies or really small specimens. Under regulations, would sectioning and embedding be considered "testing"? Any help or advice would be appreciated. Tanisha N. McKnight, HT (ASCP) Covance CLS Indianapolis Specimen Management, Anatomic Pathology From sheila_adey <@t> hotmail.com Thu Jun 12 03:24:36 2008 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Thu Jun 12 03:24:41 2008 Subject: [Histonet] Question for my Canadian colleagues In-Reply-To: <484FF144.70507@pathology.washington.edu> References: <5C0BED61F529364E86309CADEA63FEF20163F41F@TRFT-EX01.xRothGen.nhs.uk> <484FF144.70507@pathology.washington.edu> Message-ID: We have 3 Pathologists 3 Techs 0 aids We do 10,000 sugicals 1,000 cytos 100 Bone marrows appr. 7 autopsiesSheila Adey HT MLTPort Huron HospitalMichigan> Date: Wed, 11 Jun 2008 08:37:40 -0700> From: victor@pathology.washington.edu> To: Terry.Marshall@rothgen.nhs.uk> Subject: Re: [Histonet] Question for my Canadian colleagues> CC: Histonet@lists.utsouthwestern.edu> > Terry,> > The number of autopsies seems high, do all deceased get an autopsy?> > Victor> > Victor Tobias> Clinical Applications Analyst> University of Washington Medical Center> Dept of Pathology Room BB220> 1959 NE Pacific> Seattle, WA 98195> victor@pathology.washington.edu> 206-598-2792> 206-598-7659 Fax> =================================================> Privileged, confidential or patient identifiable information may be> contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments.> > > > Marshall Terry Dr, Consultant Histopathologist wrote:> > No. of pathologists/residents: 3> > No. of techs: 3> > No. of Assistants: 2> > No. of surgicals done annually (round to nearest 1000): 16000> > No. of autopsies: 740> >> > Terry> >> > -----Original Message-----> > From: histonet-bounces@lists.utsouthwestern.edu> > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Greg> > Dobbin> > Sent: 10 June 2008 18:37> > To: Histonet@lists.utsouthwestern.edu> > Subject: [Histonet] Question for my Canadian colleagues> >> > Hi folks,> > I am conducting an informal survey to find out how other labs are> > staffed (believing mine to be understaffed). > > Could you please provide me with the following numbers:> >> > No. of pathologists/residents: 3> > No. of techs: 3> > No. of Assistants: 2> > No. of surgicals done annually (round to nearest 1000): 16000> > No. of autopsies: 740> >> > Thanks in advance for any assistance you may be able to offer in this> > regard.> > Sincerely,> > Greg> >> > Greg Dobbin, R.T.> > Chief Technologist, Histology Lab> > Dept. of Laboratory Medicine,> > Queen Elizabeth Hospital,> > P.O. Box 6600> > Charlottetown, PE C1A 8T5> > Phone: (902) 894-2337> > Fax: (902) 894-2385> >> > There is some merit in doing the right thing rather badly, but> > absolutely none in doing the wrong thing excellently!> >> > -------------------------> > Statement of Confidentiality> > This message (including attachments) may contain confidential or> > privileged information intended for a specific individual or> > organization. If you have received this communication in error, please> > notify the sender immediately. If you are not the intended recipient,> > you are not authorized to use, disclose, distribute, copy, print or rely> > on this email, and should promptly delete this email from your entire> > computer system. > > > > D?claration de confidentialit?> > Le pr?sent message (y compris les annexes) peut contenir des> > renseignements confidentiels ? l'intention d'une personne ou d'un> > organisme particulier. Si vous avez re?u la pr?sente communication par> > erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes> > pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser,> > divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous> > en servir, et vous devriez l'effacer imm?diatement de votre syst?me> > informatique.> > -------------------------> >> >> > _______________________________________________> > Histonet mailing list> > Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> >> > _______________________________________________> > Histonet mailing list> > Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ From Terry.Marshall <@t> rothgen.nhs.uk Thu Jun 12 06:01:52 2008 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Thu Jun 12 05:59:31 2008 Subject: [Histonet] Question for my Canadian colleagues Message-ID: <5C0BED61F529364E86309CADEA63FEF20163F422@TRFT-EX01.xRothGen.nhs.uk> No. The large number of autopsies is due to the fact that we also act as a public mortuary for the town, and do autopsies for the Coroner. This scenario is commonplace in England. With rare exceptions, all autopsies are for the coroner. England-wide, the "hospital autopsy" is dead (as it were). Terry -----Original Message----- From: Victor Tobias [mailto:victor@pathology.washington.edu] Sent: 11 June 2008 16:38 To: Marshall Terry Dr, Consultant Histopathologist Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Question for my Canadian colleagues Terry, The number of autopsies seems high, do all deceased get an autopsy? Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Marshall Terry Dr, Consultant Histopathologist wrote: > No. of pathologists/residents: 3 > No. of techs: 3 > No. of Assistants: 2 > No. of surgicals done annually (round to nearest 1000): 16000 > No. of autopsies: 740 > > Terry > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Greg > Dobbin > Sent: 10 June 2008 18:37 > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Question for my Canadian colleagues > > Hi folks, > I am conducting an informal survey to find out how other labs are > staffed (believing mine to be understaffed). > Could you please provide me with the following numbers: > > No. of pathologists/residents: 3 > No. of techs: 3 > No. of Assistants: 2 > No. of surgicals done annually (round to nearest 1000): 16000 > No. of autopsies: 740 > > Thanks in advance for any assistance you may be able to offer in this > regard. > Sincerely, > Greg > > Greg Dobbin, R.T. > Chief Technologist, Histology Lab > Dept. of Laboratory Medicine, > Queen Elizabeth Hospital, > P.O. Box 6600 > Charlottetown, PE C1A 8T5 > Phone: (902) 894-2337 > Fax: (902) 894-2385 > > There is some merit in doing the right thing rather badly, but > absolutely none in doing the wrong thing excellently! > > ------------------------- > Statement of Confidentiality > This message (including attachments) may contain confidential or > privileged information intended for a specific individual or > organization. If you have received this communication in error, please > notify the sender immediately. If you are not the intended recipient, > you are not authorized to use, disclose, distribute, copy, print or > rely on this email, and should promptly delete this email from your > entire computer system. > > D?claration de confidentialit? > Le pr?sent message (y compris les annexes) peut contenir des > renseignements confidentiels ? l'intention d'une personne ou d'un > organisme particulier. Si vous avez re?u la pr?sente communication par > erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous > n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, > divulguer, distribuer, copier ou imprimer ce courriel ou encore de > vous en servir, et vous devriez l'effacer imm?diatement de votre > syst?me informatique. > ------------------------- > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Terry.Marshall <@t> rothgen.nhs.uk Thu Jun 12 06:15:16 2008 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Thu Jun 12 06:12:51 2008 Subject: [Histonet] Storage of Whole Mount Slides Message-ID: <5C0BED61F529364E86309CADEA63FEF20163F425@TRFT-EX01.xRothGen.nhs.uk> What use are whole mount slides now. Scan them and put them on a disc. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat.Bell@UCHSC.edu Sent: 11 June 2008 21:03 To: kenneth.metzger@aruplab.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Storage of Whole Mount Slides Try VWR. I used to get slides boxes that size from them. Pat Bell HT(ASCP) Sr. PRA Division of Medical Oncology UCHSC, Aurora, CO 303-724-3845 (lab) 303-724-3889 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Metzger, Kenneth Sent: Wednesday, June 11, 2008 11:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Storage of Whole Mount Slides Does anyone know where to get storage drawers or boxes for 3 inch x 2 inch whole mount slides? Thanks, Ken Ken Metzger HTL(ASCP) Histology Supervisor ARUP Laboratories 500 Chipeta way Salt Lake City, UT 84108 801.583.2787 ext 3101 - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Erin.Martin <@t> ucsf.edu Thu Jun 12 08:08:36 2008 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Thu Jun 12 08:10:55 2008 Subject: [Histonet] Fite stain References: <000001c8e478$8f543c90$0301a8c0@RENAD4YK9B8ABE> Message-ID: I am using the American MasterTech kit and following their procedure... Do you think that changing to a sulfuric acid differentiation would remove the false postives in granulomatous infiltrate that the acid alcohol is apparently not removing? Thanks Erin ________________________________ From: Rena Fail [mailto:renafail@bellsouth.net] Sent: Sat 7/12/2008 4:39 PM To: 'Tony Henwood'; Martin, Erin; 'histonet' Subject: RE: [Histonet] Fite stain Acid alcohol will remove your dye from those bugs requiring the peanut oil (usually Lepra Bacilli). It will also remove it from other acid fast organisms, but at a slower rate. 1% sulfuric acid differentiation gives more consistent results. Rena Fail -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Wednesday, June 11, 2008 7:20 PM To: Martin, Erin; histonet Subject: RE: [Histonet] Fite stain Your differentiation step is used in the classic ZN not a Fite. This might just be a typo but shouldn't it be 1-3% sulphuric acid (or other acid). The bugs you are trying to demonstrate with the Fite stain are acid fast but acid-alcohol labile. This won't solve the problem with you odd staining though Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Erin Sent: Wednesday, 11 June 2008 10:57 PM To: histonet Subject: [Histonet] Fite stain Good morning everyone, I am having a problem with my Fite stains. Most stain beautifully but I have had trouble with a few cases. Here are the doc's comments: "case xxxx has some lipomembranous change- and this may not really be an entirely false positive result. But there is clearly no explanation for the stuff in xxxx that stains, including some fibrin. I've never seen this before. It's not the usual round spots from inadequately rinsing off the peanut oil...." "A couple of days ago, I saw a granulomatous reaction that had tiny Fite positive "blobbos" that looked like microbes in tiny cystic spaces within the granulomas. I initially thought they might be real organisms but then I did a TB stain and proved that they were nothing. Just moments ago, XXXX had a case that was essentially identical. The Fite stain is working perfectly for ID of microbes, but it seems to have a false positive kind of reactivity that can be seen in small spaces within a granulomatous infiltrate. On an occasional basis only." Does anyone have any idea? Our procedure is 2 changes of xylene/peanut oil, air dry, rinse, carbol fuchsin for 15 min, rinse, differentiate in 1% acid alcohol, rinse, counterstain in methylene blue, air dry. Thanks in advance, _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Erin.Martin <@t> ucsf.edu Thu Jun 12 08:13:18 2008 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Thu Jun 12 08:17:03 2008 Subject: [Histonet] Hematoxylin shortage Message-ID: We also have been told that the shortage will last at least until the early autumn. Our pathologists are interested in the Phoenix Blue synthetic hematoxylin and we are supposed to be seeing some example slides next week. I can let you know what they think... From JMahoney <@t> alegent.org Thu Jun 12 08:43:14 2008 From: JMahoney <@t> alegent.org (Mahoney,Janice A) Date: Thu Jun 12 08:43:30 2008 Subject: [Histonet] RE: Hematoxylin shortage In-Reply-To: References: Message-ID: <346E5878979BA54FB4B0BFD6AD93B9B9B01F62339F@EXCHMBC1.ad.ah.local> We are in the process of working it up in my lab. So far so good. We do not have our staining times perfected yet but we think Phoenix blue has potential to be a great solution to the shortage. I'll keep you informed of the progress. Jan Mahoney Omaha -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Erin Sent: Thursday, June 12, 2008 8:13 AM To: histonet; september.amspacher@bassett.org Subject: [Histonet] Hematoxylin shortage We also have been told that the shortage will last at least until the early autumn. Our pathologists are interested in the Phoenix Blue synthetic hematoxylin and we are supposed to be seeing some example slides next week. I can let you know what they think... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. 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From pinetree110567 <@t> yahoo.com Thu Jun 12 09:30:43 2008 From: pinetree110567 <@t> yahoo.com (Jack Cates) Date: Thu Jun 12 09:30:50 2008 Subject: [Histonet] Re: Storage of Whole Mount Slides Message-ID: <544191.46545.qm@web44912.mail.sp1.yahoo.com> Ken, We use a system for all of our storage from RA Lamb (although we purchase via PSL).? System is very nice and works great for the SuperMega cassettes as well. Good luck on your search!!! Jack ------------------------------ Message: 7 Date: Wed, 11 Jun 2008 11:54:34 -0600 From: "Metzger, Kenneth" Subject: [Histonet] Storage of Whole Mount Slides To: Message-ID: ??? Content-Type: text/plain;??? charset="iso-8859-1" Does anyone know where to get storage drawers or boxes for 3 inch x 2 inch whole mount slides? Thanks, Ken Ken Metzger HTL(ASCP) Histology Supervisor ARUP Laboratories 500 Chipeta way Salt Lake City, UT 84108 801.583.2787 ext 3101 From mohs76009 <@t> yahoo.com Thu Jun 12 09:47:30 2008 From: mohs76009 <@t> yahoo.com (Matt Bancroft) Date: Thu Jun 12 09:47:38 2008 Subject: [Histonet] Slide Printer In-Reply-To: <582736990806111518j7cab0caapb1f2ba35c16a8201@mail.gmail.com> Message-ID: <158464.75392.qm@web63404.mail.re1.yahoo.com> I use thermo slides and I do not have any tissue folowing off.  I use them in mohs and in routine. --- On Wed, 6/11/08, Amos Brooks <amosbrooks@gmail.com> wrote: From: Amos Brooks <amosbrooks@gmail.com> Subject: [Histonet] Slide Printer To: rfields@gidocs.net, Stephen.Clark1@hcahealthcare.com, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Date: Wednesday, June 11, 2008, 5:18 PM Rosa, Good labeler concept, but it is a victim of a really bad proprietary issue. It is unfortunate that the only company that I have found that makes Colormark slides (or anything like them) is Erie Scientific. (Suggestions anyone?) They have terrible quality control. Tissue is constantly falling off our slides (yes, the expensive charged ones) there have been scratches down the center of many of them. We are now using adhesive in the float baths in spite of using the charged slides. I would rather deal with background staining than tissue falling off. Thermo has offered to replace bad slides, but who will replace the time it takes to cut them not to mention the wasted tissues. If I had known I would be going through this with the slides I would not have bought the labeler. I wish you the best with this, honestly, Amos Brooks Message: 5 Date: Tue, 10 Jun 2008 12:57:31 -0500 From: "Rosa Fields" <rfields@gidocs.net> Subject: RE: [Histonet] Slide printer To: "Clark Stephen - Myrtle Beach" <Stephen.Clark1@hcahealthcare.com>, <histonet@lists.utsouthwestern.edu> Message-ID: <2F2611250DCD6549AA3D96CE8AF1F01801124072@giexchange.gidocs.net> Content-Type: text/plain; charset="iso-8859-1" We have just gotten a slide etcher from Thermo, manufactured by RA Lamb, very simple to use, no ink! Small footprint to boot (no pun intended!) Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From dellav <@t> musc.edu Thu Jun 12 10:20:08 2008 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Thu Jun 12 10:20:24 2008 Subject: [Histonet] Logging in same type specimens consecutively In-Reply-To: <197710.53582.qm@web53705.mail.re2.yahoo.com> References: <197710.53582.qm@web53705.mail.re2.yahoo.com> Message-ID: The practice you are describing is recommended in the AP Quality Assurance Manual published by the CAP. It is considered poor practice to accession like specimens from different patients consecutively. The reference is Nakhleh RE, Fitzgibbons PL. Quality improvement manual in anatomic pathology, second edition. Northfield, IL: CAP; 2002 I am not aware that this is a checklist item in the accreditation requirements for AP Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Rouse Sent: Wednesday, June 11, 2008 8:41 PM To: Histonet Subject: [Histonet] Logging in same type specimens consecutively Does anyone know of a regulation by CAP that states that same type specimens should not be logged in consecutively? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bauer.Karen <@t> mayo.edu Thu Jun 12 12:12:18 2008 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen) Date: Thu Jun 12 12:12:28 2008 Subject: [Histonet] Processor Cleaning References: <197710.53582.qm@web53705.mail.re2.yahoo.com> Message-ID: Hello to all... We are having some mixed feelings about how often our processor should be cleaned and I'm curious to know what everyone else is doing. I'm sure this has been brought up on Histonet before, but I can't seem to find any pertinent findings in the archives. We are a smaller lab and process, on average, 150 blocks every night. We have 2 VIP processors (older ones) and one is only used as a back up or if we need to do an extended run on fatty or late cases. The main VIP is cleaned twice a week, on Mondays and Thursdays. The back up VIP is cleaned once a week, unless we hardly used it. By cleaned, I mean dump the first solution and rotate. Half of the staff believes that cleaning once a week would be OK, the other half wants to keep it the way it is. I've been here for 18 years and we have always cleaned it twice a week. (18 years ago, we had about one third of the specimens we do today.) Just wondering... Thanks! Karen Bauer Karen L. Bauer HT(ASCP) Department of Pathology Histology Supervisor Luther Hospital Eau Claire, WI ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. From Heather.D.Renko <@t> osfhealthcare.org Thu Jun 12 12:55:22 2008 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Thu Jun 12 12:55:47 2008 Subject: [Histonet] RE: Ameripath Message-ID: <40026EDDE64CDA47AB382C52619ACD3C0A09DFA1@pmc-rfd-mx01.intranet.osfnet.org> Anyone out there form Ameripath in PA? Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From as3323 <@t> columbia.edu Thu Jun 12 13:53:54 2008 From: as3323 <@t> columbia.edu (Anna K. Schultz) Date: Thu Jun 12 13:53:55 2008 Subject: [Histonet] Oil Red O Churukian Method Message-ID: <485170C2.1090802@columbia.edu> Hi, I am looking for a better Oil Red O protocol, and I found Gayle Callis' posting on the Churukian method--but it leaves out the amount of isopropyl alcohol in which to dissolve the .5 gm Oil Red O. I was wondering if anyone could tell me this amount. thanks a lot, Anna here is a copy of the post of the protocol from the archives: Oil Red O/Dextrin, Churukian method Fresh tissue (not prefixed) frozen sections are immersed immediately into Neutral buffered formalin for a minimum of 10 min (can be hours/days). Rinse sections with distilled water before staining. Prefixed, with NBF should be cryoprotected in 20 - 30% sucrose, mounted on Plus Charge slides and air dried for 30 min to 1 hour, or longer to insure sections stay on slide. Do not fix frozen sections with alcohol or acetone to prevent lipid removal. Protocol: 1. Immerse dry slides directly into filtered 0.5% Oil Red O in Dextrin , stain 20 minutes 2. Rinse VERY GENTLY in running tap water 3. Counterstain with Gill II hematoxylin for 20 - 30 seconds 4. Rinse gently with water, blue in Scotts tap water type bluing solution, (NOT AMMONIA WATER), rinse gently, and coverslip with aqueous mounting media. Reagents: Dissolve 0.5 gm Oil Red O in absolute isopropyl alcohol and stir overnight. Dissolve 1 gm dextrin (bacteriological grade or TYPE III (Sigma) from corn in 100 ml distilled water Working solution is 60 mls stock Oil Red O and 40 ml 1% dextrin solution Stable for months, and reported to work on paraffin sections. Reference: Gamble and Bancroft, Theory and Practice of Histological Techniques, 5th Edition, 2001 with photos. This method also published in J of Histotechnology by Charles Churukian. Go to JOH archives for journal year/volume. -- Anna K. Schultz SDRC Core A Research Technician Department of Dermatology College of Physicians and Surgeons Columbia University Vanderbilt Clinic 15-211 630 West 168^th Street New York, NY 10032 Phone: (212) 305-4954 From as3323 <@t> columbia.edu Thu Jun 12 14:06:42 2008 From: as3323 <@t> columbia.edu (Anna K. Schultz) Date: Thu Jun 12 14:06:48 2008 Subject: Never Mind Re: [Histonet] Oil Red O Churukian Method In-Reply-To: References: Message-ID: <485173C2.4090608@columbia.edu> Ugh, thanks. I thought I might be missing something, and that was it--didn't notice the .05% solution being in there. Anna Dolores Townsend wrote: > You are going to hit yourself in the head and go "duh!" Anna. If you > are making a 0.5% solution of Oil Red O, you will dilute 0.5 g of Oil > Red O in... 100 ml of Isopropy alcohol. > It's almost Friday, not to worry... > Dolores -- Anna K. Schultz SDRC Core A Research Technician Department of Dermatology College of Physicians and Surgeons Columbia University Vanderbilt Clinic 15-211 630 West 168^th Street New York, NY 10032 Phone: (212) 305-4954 From rjbuesa <@t> yahoo.com Thu Jun 12 14:29:24 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jun 12 14:29:26 2008 Subject: [Histonet] Processor Cleaning In-Reply-To: Message-ID: <422192.53595.qm@web65702.mail.ac4.yahoo.com> Whenever the reagents were scheduled to be changed I also cleaned the VIP. It something depending on the usage rate. Ren? J. "Bauer, Karen" wrote: Hello to all... We are having some mixed feelings about how often our processor should be cleaned and I'm curious to know what everyone else is doing. I'm sure this has been brought up on Histonet before, but I can't seem to find any pertinent findings in the archives. We are a smaller lab and process, on average, 150 blocks every night. We have 2 VIP processors (older ones) and one is only used as a back up or if we need to do an extended run on fatty or late cases. The main VIP is cleaned twice a week, on Mondays and Thursdays. The back up VIP is cleaned once a week, unless we hardly used it. By cleaned, I mean dump the first solution and rotate. Half of the staff believes that cleaning once a week would be OK, the other half wants to keep it the way it is. I've been here for 18 years and we have always cleaned it twice a week. (18 years ago, we had about one third of the specimens we do today.) Just wondering... Thanks! Karen Bauer Karen L. Bauer HT(ASCP) Department of Pathology Histology Supervisor Luther Hospital Eau Claire, WI ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From margaret <@t> imebinc.com Thu Jun 12 15:13:28 2008 From: margaret <@t> imebinc.com (Margaret O'Donnell) Date: Thu Jun 12 15:12:55 2008 Subject: [Histonet] Cost per slide... Message-ID: <20080612131044.7AB99C4C@dm51.mta.everyone.net> I'm trying to help out a customer with comparing costs per slide on basic H & E staining using the Sakura Prisma system vs the Ventana Symphony system. Does anyone have any information you'd be willing to share, I'd appreciate it, thanks! From sccrshlly <@t> yahoo.com Thu Jun 12 15:17:33 2008 From: sccrshlly <@t> yahoo.com (Shelly Coker) Date: Thu Jun 12 15:17:36 2008 Subject: [Histonet] Re: Glass Coverslipper mounting media Message-ID: <620930.7224.qm@web90306.mail.mud.yahoo.com> Deborah, I don't know if you have an oven available to you, but we bake our slides overnight in the 70 degree oven, and they are fine to file the next day. You can purchase metal slide trays from Cardinal (cat. # M6349-1) or CBG (check their website). Unfortunately, there is the added task of transferring from the cardboard folders to the metal ones, but this way, you won't have thousands of slides drying on your counters. Also, we don't bake the slides over the weekend because they are plenty dry by the time the weekend is over. The metal trays aren't cheap, but over the long term they may be cheaper than buying an expensive mounting media. Another option to consider if that option is not available to you is to reduce the amount of mounting medium to the very smallest volume necessary to cover the slide. I had to change the volume the machine used when I changed brands. Good luck! Shelly From rjbuesa <@t> yahoo.com Thu Jun 12 15:23:31 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jun 12 15:23:35 2008 Subject: [Histonet] Cost per slide... In-Reply-To: <20080612131044.7AB99C4C@dm51.mta.everyone.net> Message-ID: <992283.47417.qm@web65706.mail.ac4.yahoo.com> Divide costs by slides produced with each. You should find your own values because even when using automatons, there are slight variations between labs. Ren? J. Margaret O'Donnell wrote: I'm trying to help out a customer with comparing costs per slide on basic H & E staining using the Sakura Prisma system vs the Ventana Symphony system. Does anyone have any information you'd be willing to share, I'd appreciate it, thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfranci <@t> rigel.com Thu Jun 12 15:33:45 2008 From: cfranci <@t> rigel.com (Christian Franci) Date: Thu Jun 12 15:33:56 2008 Subject: [Histonet] mouse T cell markers Message-ID: <9c3f51cf44cf5eebea379806fbc97639@rigel.com> Hello, I was wondering if y'all had any thoughts on good anti- mouse T cell antibodies for paraffin IHC. Thanx a bunch, C From AnthonyH <@t> chw.edu.au Thu Jun 12 18:04:46 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Jun 12 18:05:00 2008 Subject: [Histonet] Fite stain In-Reply-To: Message-ID: No, Sulphuric acid does not differentiate as well as acid alcohol. Leprosy bacilli do not hang on to the carbol fuchsin as well as other mycobacterium so that is one reason why low pH water (ie 1% sulphuric acid) is preferred over acid alcohol. One of the other attributes of the Fite modification is the use of a vegetable oil in the dewaxing step. It is believed that the oil helps the leprae bacilli cell wall better retain the carbol fuchsin dye. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: Martin, Erin [mailto:Erin.Martin@ucsf.edu] Sent: Thursday, 12 June 2008 11:09 PM To: Rena Fail; Tony Henwood; histonet Subject: RE: [Histonet] Fite stain I am using the American MasterTech kit and following their procedure... Do you think that changing to a sulfuric acid differentiation would remove the false postives in granulomatous infiltrate that the acid alcohol is apparently not removing? Thanks Erin ________________________________ From: Rena Fail [mailto:renafail@bellsouth.net] Sent: Sat 7/12/2008 4:39 PM To: 'Tony Henwood'; Martin, Erin; 'histonet' Subject: RE: [Histonet] Fite stain Acid alcohol will remove your dye from those bugs requiring the peanut oil (usually Lepra Bacilli). It will also remove it from other acid fast organisms, but at a slower rate. 1% sulfuric acid differentiation gives more consistent results. Rena Fail -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Wednesday, June 11, 2008 7:20 PM To: Martin, Erin; histonet Subject: RE: [Histonet] Fite stain Your differentiation step is used in the classic ZN not a Fite. This might just be a typo but shouldn't it be 1-3% sulphuric acid (or other acid). The bugs you are trying to demonstrate with the Fite stain are acid fast but acid-alcohol labile. This won't solve the problem with you odd staining though Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Erin Sent: Wednesday, 11 June 2008 10:57 PM To: histonet Subject: [Histonet] Fite stain Good morning everyone, I am having a problem with my Fite stains. Most stain beautifully but I have had trouble with a few cases. Here are the doc's comments: "case xxxx has some lipomembranous change- and this may not really be an entirely false positive result. But there is clearly no explanation for the stuff in xxxx that stains, including some fibrin. I've never seen this before. It's not the usual round spots from inadequately rinsing off the peanut oil...." "A couple of days ago, I saw a granulomatous reaction that had tiny Fite positive "blobbos" that looked like microbes in tiny cystic spaces within the granulomas. I initially thought they might be real organisms but then I did a TB stain and proved that they were nothing. Just moments ago, XXXX had a case that was essentially identical. The Fite stain is working perfectly for ID of microbes, but it seems to have a false positive kind of reactivity that can be seen in small spaces within a granulomatous infiltrate. On an occasional basis only." Does anyone have any idea? Our procedure is 2 changes of xylene/peanut oil, air dry, rinse, carbol fuchsin for 15 min, rinse, differentiate in 1% acid alcohol, rinse, counterstain in methylene blue, air dry. Thanks in advance, _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From barbarahoney <@t> live.com Thu Jun 12 20:51:22 2008 From: barbarahoney <@t> live.com (Barbara Hale) Date: Thu Jun 12 20:51:29 2008 Subject: [Histonet] RE: Histonet Digest, Vol 55, Issue 21 In-Reply-To: References: Message-ID: > From: histonet-request@lists.utsouthwestern.edu> Subject: Histonet Digest, Vol 55, Issue 21> To: histonet@lists.utsouthwestern.edu> Date: Thu, 12 Jun 2008 10:00:52 -0700> > Send Histonet mailing list submissions to> histonet@lists.utsouthwestern.edu> > To subscribe or unsubscribe via the World Wide Web, visit> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> or, via email, send a message with subject or body 'help' to> histonet-request@lists.utsouthwestern.edu> > You can reach the person managing the list at> histonet-owner@lists.utsouthwestern.edu> > When replying, please edit your Subject line so it is more specific> than "Re: Contents of Histonet digest..."> > > Today's Topics:> > 1. RE: Storage of Whole Mount Slides> (Marshall Terry Dr, Consultant Histopathologist)> 2. RE: Fite stain (Martin, Erin)> 3. Hematoxylin shortage (Martin, Erin)> 4. RE: Hematoxylin shortage (Mahoney,Janice A)> 5. Re: Storage of Whole Mount Slides (Jack Cates)> 6. Re: Slide Printer (Matt Bancroft)> 7. RE: Logging in same type specimens consecutively> (Della Speranza, Vinnie)> > > ----------------------------------------------------------------------> > Message: 1> Date: Thu, 12 Jun 2008 12:15:16 +0100> From: "Marshall Terry Dr, Consultant Histopathologist"> > Subject: RE: [Histonet] Storage of Whole Mount Slides> To: , ,> > Message-ID:> <5C0BED61F529364E86309CADEA63FEF20163F425@TRFT-EX01.xRothGen.nhs.uk>> Content-Type: text/plain; charset="us-ascii"> > What use are whole mount slides now. Scan them and put them on a disc.> > Terry > > -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of> Pat.Bell@UCHSC.edu> Sent: 11 June 2008 21:03> To: kenneth.metzger@aruplab.com; histonet@lists.utsouthwestern.edu> Subject: RE: [Histonet] Storage of Whole Mount Slides> > Try VWR. I used to get slides boxes that size from them.> > Pat Bell HT(ASCP)> Sr. PRA> Division of Medical Oncology> UCHSC, Aurora, CO> 303-724-3845 (lab)> 303-724-3889 (fax)> > -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Metzger,> Kenneth> Sent: Wednesday, June 11, 2008 11:55 AM> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] Storage of Whole Mount Slides> > Does anyone know where to get storage drawers or boxes for 3 inch x 2> inch whole mount slides? Thanks,> > Ken> > Ken Metzger HTL(ASCP)> Histology Supervisor> ARUP Laboratories> 500 Chipeta way> Salt Lake City, UT 84108> 801.583.2787 ext 3101> > > - ------------------------------------------------------------------> The information transmitted by this e-mail and any included attachments> are from ARUP Laboratories and are intended only for the recipient. The> information contained in this message is confidential and may constitute> inside or non-public information under international, federal, or state> securities laws, or protected health information and is intended only> for the use of the recipient.> Unauthorized forwarding, printing, copying, distributing, or use of such> information is strictly prohibited and may be unlawful. If you are not> the intended recipient, please promptly delete this e-mail and notify> the sender of the delivery error or you may call ARUP Laboratories> Compliance Hot Line in Salt Lake City, Utah USA at (+1> (800) 522-2787 ext. 2100> > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > ------------------------------> > Message: 2> Date: Thu, 12 Jun 2008 06:08:36 -0700> From: "Martin, Erin" > Subject: RE: [Histonet] Fite stain> To: "Rena Fail" , "Tony Henwood"> , "histonet" > Message-ID:> > Content-Type: text/plain; charset=iso-8859-1> > I am using the American MasterTech kit and following their procedure... Do you think that changing to a sulfuric acid differentiation would remove the false postives in granulomatous infiltrate that the acid alcohol is apparently not removing?> > Thanks> Erin> > ________________________________> > From: Rena Fail [mailto:renafail@bellsouth.net]> Sent: Sat 7/12/2008 4:39 PM> To: 'Tony Henwood'; Martin, Erin; 'histonet'> Subject: RE: [Histonet] Fite stain> > > > Acid alcohol will remove your dye from those bugs requiring the peanut> oil (usually Lepra Bacilli). It will also remove it from other acid fast> organisms, but at a slower rate. 1% sulfuric acid differentiation gives> more consistent results.> Rena Fail> > -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony> Henwood> Sent: Wednesday, June 11, 2008 7:20 PM> To: Martin, Erin; histonet> Subject: RE: [Histonet] Fite stain> > Your differentiation step is used in the classic ZN not a Fite.> This might just be a typo but shouldn't it be 1-3% sulphuric acid (or> other acid). The bugs you are trying to demonstrate with the Fite stain> are acid fast but acid-alcohol labile.> > This won't solve the problem with you odd staining though> > Regards> > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)> Laboratory Manager & Senior Scientist> The Children's Hospital at Westmead,> Locked Bag 4001, Westmead, 2145, AUSTRALIA.> Tel: 612 9845 3306> Fax: 612 9845 3318> > > > > -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin,> Erin> Sent: Wednesday, 11 June 2008 10:57 PM> To: histonet> Subject: [Histonet] Fite stain> > > Good morning everyone, > > I am having a problem with my Fite stains. Most stain beautifully but I> have had trouble with a few cases. Here are the doc's comments:> > "case xxxx has some lipomembranous change- and this may not really be an> entirely false positive result. But there is clearly no explanation for> the stuff in xxxx that stains, including some fibrin. I've never seen> this before. It's not the usual round spots from inadequately rinsing> off the peanut oil...."> > "A couple of days ago, I saw a granulomatous reaction that had tiny Fite> positive "blobbos" that looked like microbes in tiny cystic spaces> within the granulomas. I initially thought they might be real organisms> but then I did a TB stain and proved that they were nothing. Just> moments ago, XXXX had a case that was essentially identical. The Fite> stain is working perfectly for ID of microbes, but it seems to have a> false positive kind of reactivity that can be seen in small spaces> within a granulomatous infiltrate. On an occasional basis only."> > Does anyone have any idea? Our procedure is 2 changes of xylene/peanut> oil, air dry, rinse, carbol fuchsin for 15 min, rinse, differentiate in> 1% acid alcohol, rinse, counterstain in methylene blue, air dry.> > Thanks in advance,> > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > *********************************************************************> This email and any files transmitted with it are confidential and> intended solely for the use of the individual or entity to whom they are> addressed. If you are not the intended recipient, please delete it and> notify the sender.> > Views expressed in this message and any attachments are those of the> individual sender, and are not necessarily the views of The Children's> Hospital at Westmead> > This note also confirms that this email message has been> virus scanned and although no computer viruses were detected, The> Childrens Hospital at Westmead accepts no liability for any> consequential damage resulting from email containing computer viruses.> **********************************************************************> > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > > > > ------------------------------> > Message: 3> Date: Thu, 12 Jun 2008 06:13:18 -0700> From: "Martin, Erin" > Subject: [Histonet] Hematoxylin shortage> To: "histonet" ,> september.amspacher@bassett.org> Message-ID:> > Content-Type: text/plain; charset=iso-8859-1> > We also have been told that the shortage will last at least until the early autumn. Our pathologists are interested in the Phoenix Blue synthetic hematoxylin and we are supposed to be seeing some example slides next week. I can let you know what they think...> > > > > ------------------------------> > Message: 4> Date: Thu, 12 Jun 2008 08:43:14 -0500> From: "Mahoney,Janice A" > Subject: [Histonet] RE: Hematoxylin shortage> To: "'Martin, Erin'" , histonet> , "september.amspacher@bassett.org"> > Message-ID:> <346E5878979BA54FB4B0BFD6AD93B9B9B01F62339F@EXCHMBC1.ad.ah.local>> Content-Type: text/plain; charset="us-ascii"> > We are in the process of working it up in my lab. So far so good. We do not have our staining times perfected yet but we think Phoenix blue has potential to be a great solution to the shortage.> I'll keep you informed of the progress.> Jan Mahoney> Omaha> > -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Erin> Sent: Thursday, June 12, 2008 8:13 AM> To: histonet; september.amspacher@bassett.org> Subject: [Histonet] Hematoxylin shortage> > We also have been told that the shortage will last at least until the early autumn. Our pathologists are interested in the Phoenix Blue synthetic hematoxylin and we are supposed to be seeing some example slides next week. I can let you know what they think...> > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person.> > The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation.> > > > > ------------------------------> > Message: 5> Date: Thu, 12 Jun 2008 07:30:43 -0700 (PDT)> From: Jack Cates > Subject: [Histonet] Re: Storage of Whole Mount Slides> To: histonet@lists.utsouthwestern.edu> Message-ID: <544191.46545.qm@web44912.mail.sp1.yahoo.com>> Content-Type: text/plain; charset=iso-8859-1> > Ken,> We use a system for all of our storage from RA Lamb (although we purchase via PSL). System is very nice and works great for the SuperMega cassettes as well.> Good luck on your search!!!> Jack> ------------------------------> > Message: 7> Date: Wed, 11 Jun 2008 11:54:34 -0600> From: "Metzger, Kenneth" > Subject: [Histonet] Storage of Whole Mount Slides> To: > Message-ID:> > Content-Type: text/plain; charset="iso-8859-1"> > Does anyone know where to get storage drawers or boxes for 3 inch x 2 inch whole mount slides? Thanks,> > Ken> > Ken Metzger HTL(ASCP)> Histology Supervisor> ARUP Laboratories> 500 Chipeta way> Salt Lake City, UT 84108> 801.583.2787 ext 3101> > > > > > ------------------------------> > Message: 6> Date: Thu, 12 Jun 2008 07:47:30 -0700 (PDT)> From: Matt Bancroft > Subject: Re: [Histonet] Slide Printer> To: rfields@gidocs.net, Stephen.Clark1@hcahealthcare.com,> "histonet@lists.utsouthwestern.edu"> , Amos Brooks> > Message-ID: <158464.75392.qm@web63404.mail.re1.yahoo.com>> Content-Type: text/plain; charset=us-ascii> > I use thermo slides and I do not have any tissue folowing off.  I use them in mohs and in routine.> > --- On Wed, 6/11/08, Amos Brooks <amosbrooks@gmail.com> wrote:> > From: Amos Brooks <amosbrooks@gmail.com>> Subject: [Histonet] Slide Printer> To: rfields@gidocs.net, Stephen.Clark1@hcahealthcare.com, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>> Date: Wednesday, June 11, 2008, 5:18 PM> > Rosa,> Good labeler concept, but it is a victim of a really bad proprietary> issue. It is unfortunate that the only company that I have found that makes> Colormark slides (or anything like them) is Erie Scientific. (Suggestions> anyone?) They have terrible quality control. Tissue is constantly falling> off our slides (yes, the expensive charged ones) there have been scratches> down the center of many of them.> We are now using adhesive in the float baths in spite of using the> charged slides. I would rather deal with background staining than tissue> falling off. Thermo has offered to replace bad slides, but who will replace> the time it takes to cut them not to mention the wasted tissues.> If I had known I would be going through this with the slides I would> not have bought the labeler.> > I wish you the best with this,> honestly,> Amos Brooks> > > Message: 5> Date: Tue, 10 Jun 2008 12:57:31 -0500> From: "Rosa Fields" <rfields@gidocs.net>> Subject: RE: [Histonet] Slide printer> To: "Clark Stephen - Myrtle Beach"> <Stephen.Clark1@hcahealthcare.com>,> <histonet@lists.utsouthwestern.edu>> Message-ID:> <2F2611250DCD6549AA3D96CE8AF1F01801124072@giexchange.gidocs.net>> Content-Type: text/plain; charset="iso-8859-1"> > We have just gotten a slide etcher from Thermo, manufactured by RA Lamb,> very simple to use, no ink!> Small footprint to boot (no pun intended!)> > > Rosa Fields, HT (ASCP)> Gastroenterology Specialties> Histology Supervisor> 4545 R Street> Lincoln, NE 68503> 402-465-4545> rfields@gidocs.net> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > __________________________________________________> Do You Yahoo!?> Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > ------------------------------> > Message: 7> Date: Thu, 12 Jun 2008 11:20:08 -0400> From: "Della Speranza, Vinnie" > Subject: RE: [Histonet] Logging in same type specimens consecutively> To: "'Barbara Rouse'" , Histonet> > Message-ID:> > Content-Type: text/plain; charset="iso-8859-1"> > The practice you are describing is recommended in the AP Quality Assurance Manual published by the CAP. It is considered poor practice to accession like specimens from different patients consecutively.> > The reference is> Nakhleh RE, Fitzgibbons PL. Quality improvement manual in anatomic pathology, second edition. Northfield, IL: CAP; 2002> > I am not aware that this is a checklist item in the accreditation requirements for AP> > Vinnie Della Speranza> Manager for Anatomic Pathology Services> 165 Ashley Avenue Suite 309> Charleston, South Carolina 29425> Tel: (843) 792-6353> Fax: (843) 792-8974> > -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Rouse> Sent: Wednesday, June 11, 2008 8:41 PM> To: Histonet> Subject: [Histonet] Logging in same type specimens consecutively> > Does anyone know of a regulation by CAP that states that same type specimens should not be logged in consecutively? Thanks> > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > ------------------------------> > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > End of Histonet Digest, Vol 55, Issue 21> **************************************** _________________________________________________________________ Enjoy 5 GB of free, password-protected online storage. http://www.windowslive.com/skydrive/overview.html?ocid=TXT_TAGLM_WL_Refresh_skydrive_062008 From John.Spair <@t> multicare.org Fri Jun 13 01:25:21 2008 From: John.Spair <@t> multicare.org (John Spair) Date: Fri Jun 13 01:26:09 2008 Subject: [Histonet] RE: Histonet Digest, Vol 55, Issue 21 References: <644F89521RC150272-01@MMS_multicare.org> Message-ID: <61A9977919846C479389493BAE2517CA015309A0@MHSEXMBX1.multicare.org> No CAP regulation about this, it however is a good practice unless you only process one type of specimen. Then you should implement a second identifier, such as putting patient initials on the cassettes as well....All of this really helps when you encounter a problem. Thank you. ' From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu ] On Behalf Of Barbara Rouse Sent: Wednesday, June 11, 2008 8:41 PM To: Histonet Subject: [Histonet] Logging in same type specimens consecutively Does anyone know of a regulation by CAP that states that same type specimens should not be logged in consecutively? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet John Spair, Manager Pathology Services LABORATORIES Northwest MultiCare Health System PO Box 5299, Tacoma WA 98415 Phone: 253-403-6090 Fax: 253-403-1357 "MMS " made the following annotations. ------------------------------------------------------------------------------ NOTICE: This e-mail and the attachments hereto, if any, may contain privileged and/or confidential information. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, you are hereby notified that any examination, distribution or copying of this e-mail and the attachments hereto, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by email or telephone and permanently delete this e-mail and the attachments hereto, if any, and destroy any printout thereof. MultiCare Health System, Tacoma, WA 98415 (253) 403-1000. ============================================================================== From b-frederick <@t> northwestern.edu Fri Jun 13 08:08:30 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Jun 13 08:08:45 2008 Subject: [Histonet] RE: Histonet Digest, Vol 55, Issue 21 In-Reply-To: <61A9977919846C479389493BAE2517CA015309A0@MHSEXMBX1.multicare.org> Message-ID: <000501c8cd56$966c7170$d00f7ca5@lurie.northwestern.edu> I've worked at large institutions where even if the samples are not grossed together, the slides may end up adjacent to each other on a slide tray for the pathologist. Many hospitals now place a marker in the space between all cases, not just those that are the same type of tissue (it says "end of case and is fluorescent shade of paper. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Spair Sent: Friday, June 13, 2008 1:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 55, Issue 21 No CAP regulation about this, it however is a good practice unless you only process one type of specimen. Then you should implement a second identifier, such as putting patient initials on the cassettes as well....All of this really helps when you encounter a problem. Thank you. ' From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu ] On Behalf Of Barbara Rouse Sent: Wednesday, June 11, 2008 8:41 PM To: Histonet Subject: [Histonet] Logging in same type specimens consecutively Does anyone know of a regulation by CAP that states that same type specimens should not be logged in consecutively? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet John Spair, Manager Pathology Services LABORATORIES Northwest MultiCare Health System PO Box 5299, Tacoma WA 98415 Phone: 253-403-6090 Fax: 253-403-1357 "MMS " made the following annotations. ---------------------------------------------------------------------------- -- NOTICE: This e-mail and the attachments hereto, if any, may contain privileged and/or confidential information. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, you are hereby notified that any examination, distribution or copying of this e-mail and the attachments hereto, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by email or telephone and permanently delete this e-mail and the attachments hereto, if any, and destroy any printout thereof. MultiCare Health System, Tacoma, WA 98415 (253) 403-1000. ============================================================================ == From HornHV <@t> archildrens.org Fri Jun 13 08:38:05 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Jun 13 08:38:04 2008 Subject: [Histonet] Logging in same type specimens consecutively In-Reply-To: References: <197710.53582.qm@web53705.mail.re2.yahoo.com> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82CBD@EMAIL.archildrens.org> I don't understand why it is considered poor practice to accession like specimens. Every part of the clinical lab does this every day. We get so many biopsies of the same kind we cannot accession them without accessioning them consecutively. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Thursday, June 12, 2008 10:20 AM To: 'Barbara Rouse'; Histonet Subject: RE: [Histonet] Logging in same type specimens consecutively The practice you are describing is recommended in the AP Quality Assurance Manual published by the CAP. It is considered poor practice to accession like specimens from different patients consecutively. The reference is Nakhleh RE, Fitzgibbons PL. Quality improvement manual in anatomic pathology, second edition. Northfield, IL: CAP; 2002 I am not aware that this is a checklist item in the accreditation requirements for AP Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Rouse Sent: Wednesday, June 11, 2008 8:41 PM To: Histonet Subject: [Histonet] Logging in same type specimens consecutively Does anyone know of a regulation by CAP that states that same type specimens should not be logged in consecutively? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From mpence <@t> grhs.net Fri Jun 13 08:43:33 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Jun 13 08:43:45 2008 Subject: [Histonet] Logging in same type specimens consecutively In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82CBD@EMAIL.archildrens.org> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3864@IS-E2K3.grhs.net> I don't think it should be called poor practice, because as it has been stated many time here, some places have no choice but to log same specimens back to back. It should be called not "best practice". I think everyone would agree that if you can put a different case between like specimens that would be "best practice". Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Friday, June 13, 2008 8:38 AM To: Della Speranza, Vinnie; Barbara Rouse; Histonet Subject: RE: [Histonet] Logging in same type specimens consecutively I don't understand why it is considered poor practice to accession like specimens. Every part of the clinical lab does this every day. We get so many biopsies of the same kind we cannot accession them without accessioning them consecutively. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Thursday, June 12, 2008 10:20 AM To: 'Barbara Rouse'; Histonet Subject: RE: [Histonet] Logging in same type specimens consecutively The practice you are describing is recommended in the AP Quality Assurance Manual published by the CAP. It is considered poor practice to accession like specimens from different patients consecutively. The reference is Nakhleh RE, Fitzgibbons PL. Quality improvement manual in anatomic pathology, second edition. Northfield, IL: CAP; 2002 I am not aware that this is a checklist item in the accreditation requirements for AP Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Rouse Sent: Wednesday, June 11, 2008 8:41 PM To: Histonet Subject: [Histonet] Logging in same type specimens consecutively Does anyone know of a regulation by CAP that states that same type specimens should not be logged in consecutively? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ****************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KjLand <@t> usi.edu Fri Jun 13 08:44:11 2008 From: KjLand <@t> usi.edu (Land, Kimberly J) Date: Fri Jun 13 08:46:47 2008 Subject: [Histonet] Mouse Mammary Gland Message-ID: <5777F3052F84F14296F7B9653A51F24C11ABE4@emailnew.usi.edu> Dear Histonetters, In the very near future, I will be preparing whole mounts of mouse mammary glands. The plan is to use Carnoy's fixative and to stain with Carmine alum overnight. That part of the procedure I think I can handle. My main concern is that afterwards, I am to embed in paraffin for H&E staining. Has anyone out there done this before?? Does the Carmine alum interfere with the H&E? How thick do the sections need to be? I am used to histology on mouse thyroids, so this is a totally new thing for me. Any help would be appreciated. Thank you, Kim Lohe University of Southern Indiana 8600 University Blvd. Evansville, IN 47712 From TMcNemar <@t> lmhealth.org Fri Jun 13 09:00:32 2008 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Jun 13 09:00:36 2008 Subject: [Histonet] Logging in same type specimens consecutively In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82CBD@EMAIL.archildrens.org> Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F584@lmhsmail.lmhealth.org> We still handwrite the numbers on our slides. Although we make few labeling errors, it does happen. It just makes it easier to correct those mistakes. It also reduces the chance of carryover contamination at the gross bench. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Horn, Hazel V Sent: Friday, June 13, 2008 9:38 AM To: Della Speranza, Vinnie; Barbara Rouse; Histonet Subject: RE: [Histonet] Logging in same type specimens consecutively I don't understand why it is considered poor practice to accession like specimens. Every part of the clinical lab does this every day. We get so many biopsies of the same kind we cannot accession them without accessioning them consecutively. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Thursday, June 12, 2008 10:20 AM To: 'Barbara Rouse'; Histonet Subject: RE: [Histonet] Logging in same type specimens consecutively The practice you are describing is recommended in the AP Quality Assurance Manual published by the CAP. It is considered poor practice to accession like specimens from different patients consecutively. The reference is Nakhleh RE, Fitzgibbons PL. Quality improvement manual in anatomic pathology, second edition. Northfield, IL: CAP; 2002 I am not aware that this is a checklist item in the accreditation requirements for AP Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Rouse Sent: Wednesday, June 11, 2008 8:41 PM To: Histonet Subject: [Histonet] Logging in same type specimens consecutively Does anyone know of a regulation by CAP that states that same type specimens should not be logged in consecutively? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Fri Jun 13 09:58:03 2008 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Fri Jun 13 09:56:18 2008 Subject: [Histonet] Logging in same type specimens consecutively In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A3864@IS-E2K3.grhs.net> References: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82CBD@EMAIL.archildrens.org> <661949901A768E4F9CC16D8AF8F2838C017A3864@IS-E2K3.grhs.net> Message-ID: Mike, I responded to one of our colleagues back channel that the dilemma in inserting different specimens is likely the reason the CAP has not made this a requirement. I don't care what you call it, the semantics do not change the outcome if two patient samples are mixed up and cannot be distinguished at the microscope. Should that happen I doubt anyone including your risk management department will care what you called it. And please understand that it was not my intent to sound like I was preaching. We have the same challenges here that others have. I was simply intending to answer the OP's question. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Friday, June 13, 2008 9:44 AM To: Horn, Hazel V; Della Speranza, Vinnie; Barbara Rouse; Histonet Subject: RE: [Histonet] Logging in same type specimens consecutively I don't think it should be called poor practice, because as it has been stated many time here, some places have no choice but to log same specimens back to back. It should be called not "best practice". I think everyone would agree that if you can put a different case between like specimens that would be "best practice". Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Friday, June 13, 2008 8:38 AM To: Della Speranza, Vinnie; Barbara Rouse; Histonet Subject: RE: [Histonet] Logging in same type specimens consecutively I don't understand why it is considered poor practice to accession like specimens. Every part of the clinical lab does this every day. We get so many biopsies of the same kind we cannot accession them without accessioning them consecutively. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Thursday, June 12, 2008 10:20 AM To: 'Barbara Rouse'; Histonet Subject: RE: [Histonet] Logging in same type specimens consecutively The practice you are describing is recommended in the AP Quality Assurance Manual published by the CAP. It is considered poor practice to accession like specimens from different patients consecutively. The reference is Nakhleh RE, Fitzgibbons PL. Quality improvement manual in anatomic pathology, second edition. Northfield, IL: CAP; 2002 I am not aware that this is a checklist item in the accreditation requirements for AP Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Rouse Sent: Wednesday, June 11, 2008 8:41 PM To: Histonet Subject: [Histonet] Logging in same type specimens consecutively Does anyone know of a regulation by CAP that states that same type specimens should not be logged in consecutively? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ****************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From EdieL <@t> fmchealth.org Fri Jun 13 09:57:26 2008 From: EdieL <@t> fmchealth.org (Edie Lehman) Date: Fri Jun 13 09:57:32 2008 Subject: [Histonet] Tissue Processors Message-ID: <3C25F9EF8E8DF84DBBB1884C297E8AF40379079F@ex03.fmchealth.org> First many thanks to all who responded to my request for autostainer information. It has been most helpful. Second, could I please get some input on Tissue Processors? Anyone using the microwave technology, and if so, how do you like it? Do the pathologist like it? Again any advice is appreciated. Thanks in advance, Edie Lehman MT(ASCP) Anatomical Pathology Supervisor Fairfield Medical Center EdieL@fmchealth.org (740)687-8807 "Confidentiality Notice: This e-mail message, including any attachments is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review; use; disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message." From RSRICHMOND <@t> aol.com Fri Jun 13 10:31:34 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Fri Jun 13 10:31:43 2008 Subject: [Histonet] Re: Logging in same type specimens consecutively Message-ID: Avoid logging in specimens of the same type (prostate, breast, colon biopsies for example) consecutively. If it can't be avoided (say you get four stereotactic breast biopsy specimens at the end of the day) then go ahead and number them consecutively. I did some work at a specialty prostate lab a few years ago, and they took extraordinary care to distinguish their specimens. Ten different colors of cassettes, ten different colors of slides, and several other things. Multiple colors of cassettes are easily available at no additional cost (except the increase in inventory), and they're greatly underutilized by pathology services. Bob Richmond Samurai Pathologist Knoxville TN From lelmgren <@t> sunriselab.com Fri Jun 13 10:36:05 2008 From: lelmgren <@t> sunriselab.com (Laurie Elmgren) Date: Fri Jun 13 10:36:09 2008 Subject: [Histonet] Processor Cleaning In-Reply-To: <422192.53595.qm@web65702.mail.ac4.yahoo.com> References: <422192.53595.qm@web65702.mail.ac4.yahoo.com> Message-ID: <4A672C6AE0402D4A89ECE29E8A4B47E3010F4818@MailPDC.sunriselab.com> Happy Friday, We have the VIP 5 and run about 240-280cassettes through six nights a week. Our cleaning schedule is: all solutions are changed every Tuesday. Thursday and Saturday the solutions are rotated. Since we have been following this schedule, the cutting problems have been minimal. Laurie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, June 12, 2008 3:29 PM To: Bauer, Karen; Histonet Subject: Re: [Histonet] Processor Cleaning Whenever the reagents were scheduled to be changed I also cleaned the VIP. It something depending on the usage rate. Ren? J. "Bauer, Karen" wrote: Hello to all... We are having some mixed feelings about how often our processor should be cleaned and I'm curious to know what everyone else is doing. I'm sure this has been brought up on Histonet before, but I can't seem to find any pertinent findings in the archives. We are a smaller lab and process, on average, 150 blocks every night. We have 2 VIP processors (older ones) and one is only used as a back up or if we need to do an extended run on fatty or late cases. The main VIP is cleaned twice a week, on Mondays and Thursdays. The back up VIP is cleaned once a week, unless we hardly used it. By cleaned, I mean dump the first solution and rotate. Half of the staff believes that cleaning once a week would be OK, the other half wants to keep it the way it is. I've been here for 18 years and we have always cleaned it twice a week. (18 years ago, we had about one third of the specimens we do today.) Just wondering... Thanks! Karen Bauer Karen L. Bauer HT(ASCP) Department of Pathology Histology Supervisor Luther Hospital Eau Claire, WI ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmilne <@t> bccancer.bc.ca Fri Jun 13 10:40:50 2008 From: kmilne <@t> bccancer.bc.ca (Milne, Katy) Date: Fri Jun 13 10:40:58 2008 Subject: [Histonet] RE: mouse T cell markers In-Reply-To: References: Message-ID: <07979E76B0869D4E8C9FE4AA9FC06578046691C9@srvex03.phsabc.ehcnet.ca> Hi Christian, Depends on what you're looking for. LabVision's CD3 rabbit monoclonal is great as a pan T cell marker (Clone SP7). E-bioscience has a good FoxP3 RtMAb for Tregs (clone FJK-16). Other than that it gets a little sketchy finding good antibodies for mouse FFPE tissue. I haven't had any luck with other CD markers that I have been interested in (CD4, CD8 etc) looking through the archives, you'll probably find it's a fruitless search! If you would like granzyme B for activated cytolytic cells (and NKs), we use one from Abcam (I think it's cat# ab4059 but I don't have the vial in front of me). Good luck! Katy Milne Deeley Research Centre BC Cancer Agency Message: 9 Date: Thu, 12 Jun 2008 13:33:45 -0700 From: Christian Franci Subject: [Histonet] mouse T cell markers To: histonet@lists.utsouthwestern.edu Message-ID: <9c3f51cf44cf5eebea379806fbc97639@rigel.com> Content-Type: text/plain; charset=US-ASCII; format=flowed Hello, I was wondering if y'all had any thoughts on good anti- mouse T cell antibodies for paraffin IHC. Thanx a bunch, C From rjbuesa <@t> yahoo.com Fri Jun 13 10:46:44 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 13 10:46:48 2008 Subject: [Histonet] Tissue Processors In-Reply-To: <3C25F9EF8E8DF84DBBB1884C297E8AF40379079F@ex03.fmchealth.org> Message-ID: <620876.45197.qm@web65702.mail.ac4.yahoo.com> When considering MWO processing based on reducing the TAT, think on all the other steps. Under separate cover I am sending you an article on the subject. Ren? J. Edie Lehman wrote: First many thanks to all who responded to my request for autostainer information. It has been most helpful. Second, could I please get some input on Tissue Processors? Anyone using the microwave technology, and if so, how do you like it? Do the pathologist like it? Again any advice is appreciated. Thanks in advance, Edie Lehman MT(ASCP) Anatomical Pathology Supervisor Fairfield Medical Center EdieL@fmchealth.org (740)687-8807 "Confidentiality Notice: This e-mail message, including any attachments is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review; use; disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Fri Jun 13 10:50:57 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Jun 13 10:51:05 2008 Subject: [Histonet] Re: Logging in same type specimens consecutively In-Reply-To: References: Message-ID: <982A0A9461F9BF438C7B19A6E425A3831E1C5A@ITSSSXM01V6.one.ads.che.org> We make a marker with agar and put a different color in each case. It is embedded and cut right along with the tissue, so it is a good trail. The pathologists also document it in the report as a QA measure. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Friday, June 13, 2008 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Logging in same type specimens consecutively Avoid logging in specimens of the same type (prostate, breast, colon biopsies for example) consecutively. If it can't be avoided (say you get four stereotactic breast biopsy specimens at the end of the day) then go ahead and number them consecutively. I did some work at a specialty prostate lab a few years ago, and they took extraordinary care to distinguish their specimens. Ten different colors of cassettes, ten different colors of slides, and several other things. Multiple colors of cassettes are easily available at no additional cost (except the increase in inventory), and they're greatly underutilized by pathology services. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From TMcNemar <@t> lmhealth.org Fri Jun 13 10:51:49 2008 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Jun 13 10:51:56 2008 Subject: [Histonet] Re: Logging in same type specimens consecutively In-Reply-To: Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F585@lmhsmail.lmhealth.org> We often skip numbers at the end of the day. We then pick those numbers up the next day with dissimilar specimens. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Robert Richmond Sent: Friday, June 13, 2008 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Logging in same type specimens consecutively Avoid logging in specimens of the same type (prostate, breast, colon biopsies for example) consecutively. If it can't be avoided (say you get four stereotactic breast biopsy specimens at the end of the day) then go ahead and number them consecutively. I did some work at a specialty prostate lab a few years ago, and they took extraordinary care to distinguish their specimens. Ten different colors of cassettes, ten different colors of slides, and several other things. Multiple colors of cassettes are easily available at no additional cost (except the increase in inventory), and they're greatly underutilized by pathology services. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From zodiac29 <@t> comcast.net Fri Jun 13 10:52:01 2008 From: zodiac29 <@t> comcast.net (zodiac29@comcast.net) Date: Fri Jun 13 10:52:11 2008 Subject: [Histonet] Weekend differential Message-ID: <061320081552.11149.485297A10005FAA200002B8D2215578674C7CD0C0E070B0196@comcast.net> Hello To all, I just wanted to know what the other labs out there are paying for their weekend differential. My lab usually isn't open on saturdays, but there is a chance that on some saturdays I might have to come in to process a Stat. Just wanted some feedback to bring back to my employer. Thanks Jenny From HornHV <@t> archildrens.org Fri Jun 13 11:26:11 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Jun 13 11:26:07 2008 Subject: [Histonet] Re: Logging in same type specimens consecutively In-Reply-To: References: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82CC1@EMAIL.archildrens.org> Actually this is exactly what we do. Each biopsy case is put in colored cassettes and that color is dictated by our resident. At the microtome we use the same color slides as the cassette color. We rotate the colors with each case all day long during accessioning. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Friday, June 13, 2008 10:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Logging in same type specimens consecutively Avoid logging in specimens of the same type (prostate, breast, colon biopsies for example) consecutively. If it can't be avoided (say you get four stereotactic breast biopsy specimens at the end of the day) then go ahead and number them consecutively. I did some work at a specialty prostate lab a few years ago, and they took extraordinary care to distinguish their specimens. Ten different colors of cassettes, ten different colors of slides, and several other things. Multiple colors of cassettes are easily available at no additional cost (except the increase in inventory), and they're greatly underutilized by pathology services. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From jcline <@t> wchsys.org Fri Jun 13 11:38:36 2008 From: jcline <@t> wchsys.org (Joyce Cline) Date: Fri Jun 13 11:38:41 2008 Subject: [Histonet] Processor Cleaning In-Reply-To: Message-ID: We change our two formalins after two days of use, we change our purge after three days of use. On Monday our 80% is changed, our 95%, 100% and our substitute: 1st one is changed and the other rotated up. We process about 150 to 200 blocks per day. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen Sent: Thursday, June 12, 2008 1:12 PM To: Histonet Subject: [Histonet] Processor Cleaning Hello to all... We are having some mixed feelings about how often our processor should be cleaned and I'm curious to know what everyone else is doing. I'm sure this has been brought up on Histonet before, but I can't seem to find any pertinent findings in the archives. We are a smaller lab and process, on average, 150 blocks every night. We have 2 VIP processors (older ones) and one is only used as a back up or if we need to do an extended run on fatty or late cases. The main VIP is cleaned twice a week, on Mondays and Thursdays. The back up VIP is cleaned once a week, unless we hardly used it. By cleaned, I mean dump the first solution and rotate. Half of the staff believes that cleaning once a week would be OK, the other half wants to keep it the way it is. I've been here for 18 years and we have always cleaned it twice a week. (18 years ago, we had about one third of the specimens we do today.) Just wondering... Thanks! Karen Bauer Karen L. Bauer HT(ASCP) Department of Pathology Histology Supervisor Luther Hospital Eau Claire, WI ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From godsgalnow <@t> aol.com Fri Jun 13 11:51:38 2008 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Fri Jun 13 11:51:45 2008 Subject: [Histonet] Weekend differential In-Reply-To: <061320081552.11149.485297A10005FAA200002B8D2215578674C7CD0C0E070B0196@comcast.net> References: <061320081552.11149.485297A10005FAA200002B8D2215578674C7CD0C0E070B0196@comcast.net> Message-ID: <8CA9B8CE1DB360E-B2C-3AE@Webmail-mg06.sim.aol.com> Differential....what is that?? I wsan't aware that places still did that. Roxanne -----Original Message----- From: zodiac29@comcast.net To: histonet@lists.utsouthwestern.edu Sent: Fri, 13 Jun 2008 11:52 am Subject: [Histonet] Weekend differential Hello To all, I just wanted to know what the other labs out there are paying for their weekend differential. My lab usually isn't open on saturdays, but there is a chance that on some saturdays I might have to come in to process a Stat. Just wanted some feedback to bring back to my employer. Thanks Jenny _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From m.mihalik <@t> comcast.net Fri Jun 13 11:56:31 2008 From: m.mihalik <@t> comcast.net (m.mihalik@comcast.net) Date: Fri Jun 13 11:56:37 2008 Subject: [Histonet] Re: Logging in same type specimens consecutively Message-ID: <061320081656.27406.4852A6BE0009497E00006B0E22068246930507040E080703D203@comcast.net> I'd like to comment on this issue if I could, but everyone please be aware of my perspective as an information systems provider. We spend a LOT of time IN labs, not in offices. This issue comes up quite often. Everyone should be applauded for their efforts at patient quality and 'doing the right thing', but I would assert that with the proper procedures and information system support, that this policy is not as necessary as it used to be. IF you have a system where you can accession a sample ONE at a time by scanning the bar code on the requisition which immediately prints a specimen label with a bar code, which is immeditately applied to the specimen then you have positively and uniquely id'd the specimen. Follow that up with bar coded blocks, which are then scanned to produce the applicable bar coded slide labels (again, done one case at a time -- scanned block, slide labels printed at the cutting station, labels applied to the slide). Finally, when the pathologist or cytotechnologist gets the slide, they scan the slide barcode to enter the results and see the history on the patient. The key to the entire process is accessioning one case at a time and once that is done, because of all the barcodes, all the other error points are reduced. ...at least that's my thinking. I'm curious what you all think. Mike Mihalik PathView Systems -------------- Original message -------------- From: "Horn, Hazel V" > Actually this is exactly what we do. Each biopsy case is put in > colored cassettes and that color is dictated by our resident. At the > microtome we use the same color slides as the cassette color. We > rotate the colors with each case all day long during accessioning. > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Histology > Arkansas Children's Hospital > 800 Marshall Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax 501.364.3155 > > visit us on the web at: www.archildrens.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert > Richmond > Sent: Friday, June 13, 2008 10:32 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Logging in same type specimens consecutively > > Avoid logging in specimens of the same type (prostate, breast, colon > biopsies for example) consecutively. If it can't be avoided (say you > get four stereotactic breast biopsy specimens at the end of the day) > then go ahead and number them consecutively. > > I did some work at a specialty prostate lab a few years ago, and they > took extraordinary care to distinguish their specimens. Ten different > colors of cassettes, ten different colors of slides, and several other > things. Multiple colors of cassettes are easily available at no > additional cost (except the increase in inventory), and they're > greatly underutilized by pathology services. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ******************************************************************************** > ******************************************************************************** > ******************************************************************************** > ******************************************************************************** > ******************************************************************************** > ******************************************************************************** > ******************************************************************************** > ********************************************************************** > The information contained in this message may be privileged and confidential > and protected from disclosure. If the reader of this message is not the > intended recipient, or an employee or agent responsible for delivering this > message to the intended recipient, you are hereby notified that any > dissemination, distribution or copying of this communication is strictly > prohibited. If you have received this communication in error, please notify > us immediately by replying to the message and deleting it from your computer. > Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rhbrown <@t> histocs.com Fri Jun 13 12:49:22 2008 From: rhbrown <@t> histocs.com (histocs) Date: Fri Jun 13 12:49:29 2008 Subject: [Histonet] Looking for sevice manual again Message-ID: <000c01c8cd7d$d0945fd0$6500a8c0@LHBLION> I am still looking for a copy of the service manual for my TP1050. I have the users manual but I have some minor repairs to make and therefore need to have a service manual. If you would be willing to make a copy I would be happy to pay for it. Contact me at 360-966-7300 or rhbrown1@histocs.com LeRoy Brown HT(ASCP)HTL HCS Everson, wA 98247 From rhbrown <@t> histocs.com Fri Jun 13 13:00:11 2008 From: rhbrown <@t> histocs.com (histocs) Date: Fri Jun 13 13:00:19 2008 Subject: [Histonet] HM330 microtome parts needed Message-ID: <001301c8cd7f$5375d130$6500a8c0@LHBLION> Does anyone have a Microm, HM330, microtome that might be good for parts? I need the outer plastic housing parts. The one I have was dropped and the plastic shell is broke. Any ideas on who might sell these parts? Many thanks. LeRoy Brown HT(ASCP)HTL HCS 360-966-7300 From mickie25 <@t> netzero.net Fri Jun 13 14:42:11 2008 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Fri Jun 13 14:43:00 2008 Subject: [Histonet] HM330 microtome parts needed In-Reply-To: <001301c8cd7f$5375d130$6500a8c0@LHBLION> References: <001301c8cd7f$5375d130$6500a8c0@LHBLION> Message-ID: Dear Leroy, You might try Belair Instrument Co. Their phone number is 600-783-9424. Good Luck! Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histocs Sent: Friday, June 13, 2008 11:00 AM To: Histonet Subject: [Histonet] HM330 microtome parts needed Does anyone have a Microm, HM330, microtome that might be good for parts? I need the outer plastic housing parts. The one I have was dropped and the plastic shell is broke. Any ideas on who might sell these parts? Many thanks. LeRoy Brown HT(ASCP)HTL HCS 360-966-7300 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bill501 <@t> mindspring.com Fri Jun 13 15:19:22 2008 From: bill501 <@t> mindspring.com (Bill) Date: Fri Jun 13 15:19:27 2008 Subject: [Histonet] Re: Logging in same type specimens consecutively In-Reply-To: <982A0A9461F9BF438C7B19A6E425A3831E1C5A@ITSSSXM01V6.one.ads.che.org> References: <982A0A9461F9BF438C7B19A6E425A3831E1C5A@ITSSSXM01V6.one.ads.che.org> Message-ID: At 11:50 AM -0400 6/13/08, Weems, Joyce wrote: >We make a marker with agar and put a different color in each case. It is >embedded and cut right along with the tissue, so it is a good trail. The >pathologists also document it in the report as a QA measure. Good idea Joyce. Could you provide more info on the technique. How do you color the agar etc. Bill From sccrshlly <@t> yahoo.com Fri Jun 13 15:47:26 2008 From: sccrshlly <@t> yahoo.com (Shelly Coker) Date: Fri Jun 13 15:47:30 2008 Subject: [Histonet] Re: Cleaning Processors Message-ID: <400924.36660.qm@web90302.mail.mud.yahoo.com> I worked in a very large reference lab with the old VIP's, and the gold standard was 300 blocks, and the solutions had to be changed (rotated). That standard would explain why you currently are changing every other day (about 300 blocks). I will say this...when the processors weren't changed as scheduled (occasionally the ball got dropped) we immediately noticed marked difficulty with cutting, and had occasions when the staining was adversely affected as well. We used a hydrometer to check the alcohols as we rotated them, and the amount of carry over surprised me. Occassionally the 100% alcohol would be as low as 95% after the processing run. Had we not changed the alcohols per our protocol, that 5% could be disasterous the next day. Good luck! Shelly From gayle.callis <@t> bresnan.net Fri Jun 13 17:06:52 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Fri Jun 13 17:06:52 2008 Subject: [Histonet] accessing messages in archives, the need for authentication Message-ID: <001201c8cda1$c91ed950$6401a8c0@DHXTS541> Has anyone encountered a window that arises saying one needs to go to "LOGS" at http://www.histosearch.com followed by a username and password to authenticate and access information from the Histonet archive messages. This happened everytime I tried to open the messages with subject: "RE:[Histonet] Re: Logging in same type specimens consecutively" Apparently Histonet archives doesn't like the words logging in????? And this doesn't happen with any other messages in the Archives. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT From lpwenk <@t> sbcglobal.net Sat Jun 14 06:45:08 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sat Jun 14 06:45:29 2008 Subject: [Histonet] Legality of work In-Reply-To: <163789.93086.qm@web32506.mail.mud.yahoo.com> Message-ID: <001101c8ce14$18f9c8f0$eff62d4b@HPPav2> Sorry I didn't get in on this discussion earlier. Have been without electricity since the weekend. I wanted to bring up a couple of other issues. 1. NAACLS Requirements: NAACLS is authorized by the US government to accredit POST-SECONDARY laboratory programs, in other words, after high school. So first of all, to be in a NAACLS program, the person has to have graduated from high school. All the NAACLS programs that have on-line classes would have to have the candidate to be a minimum of a high school graduate. So this 15 year old students wouldn't meet this requirement. Second, under the NAACLS requirement, anyone in a NAACLS program must have had as minimum education, 1 class in each - biology, chemistry and math. This could be in high school, or it could be in college. I doubt that a 15 year old would have had the chemistry requirement, at the very least. 2. NAACLS Programs Requirements: After having said #1, each NAALCS program can set their own standard at or higher than NAACLS standards. So a NAACLS HT program could require that the one biology/chem/math courses be college level, or could say that the biology class must be college anatomy and physiology. Or that the person must have an associate degree. (Just to let you know, my program (not on-line) requires an associate degree, anatomy, physiology, microbiology, 2 chemistry, and intermediate college algebra as minimum requirements.) So, if anyone is interested in any of the HT on-line programs, the candidate would have to meet not only the NAACLS minimum education requirements but also the Program's minimum education requirements. This student would not. 3. NAACLS vs OJT routes of entry: Just a reminder, in order to take the HT exam, this student either has to complete a NAACLS HT program (which as of right now she can't get into because of not having the high school diploma and not having all the bio/chem/math classes), nor can she take the HT exam via the on-the-job (OJT) route, as she does not have an associate degree with 12 credits of biology and chemistry. 4. Appearance, Legally: One last thing, which I may get blasted for. Yes, I love the idea of job shadowing, and think it is great in terms of students working with already diagnosed tissue that was going to be disposed of, to set up their own study sets/portfolios. However, having a student work on real patient tissue that is involved in the diagnosis - well, let's look at it from a lawyer's point of view. Let's say this 15 year old high school student makes a mistake - embeds a skin wrong, cuts through the tissue, makes up a stain incorrectly. We've all done it, I know, but what would the lawyer for the patient say to a jury about this situation? That is pathologist hired a underage high school student -(that according to the originator of this chain) it is illegal for a 15 year old to be in a lab working with chemical and BBP. - This student is not eligible to get into a NAACLS HT program - not a high school graduate (or higher) and without the correct bio/chem/math requirements. - This student is not eligible to take the ASCP registry exam via the OJT route - no associate degree with 12 credits of bio/chem This lawyer is going to tell the jury that this lab hired a student illegally and who could not meet the national standards for training or taking a national certification exam. And that the lab and director did this knowingly. (after all, the regulations and standards are available, and trust me, a lawyer would google this). So what do you think a jury would do? What would their verdict be? Would it go for or against the lab and the director? Remember, this is the JURY'S PERCEPTION (not what we as histotechs think/know). Personally, I think the verdict would go against the lab and director, and let's hike up the amount of money the director/lab has to pay the patient and their family. So I might recommend that anyone involved in a situation like this - purchase malpractice insurance - techs included. Peggy A. Wenk, HTL(ASCP)SLS Schools of Histotechnology (which is NAACLS accredited - my disclaimer) William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Karla Arrington Sent: Wednesday, June 04, 2008 7:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Legality of work Histo's: ? I have roughly 2 dilemmas.? The first is of a legal matter.? The Pathologist for a week has had his daughter (15 years old), helping me out in the histology lab.? He wants her to get training from me, then do the on-line HT program.? She has handled chemicals and reagents.? I am very uncomfortable with this.? I have called the Child Labor Laws department for our state and it is illegal for a 15 year to be in a laboratory. Needless to say working with blood borne pathogens.? My most concern is the following.? As her "teacher", can I or other co-workers can be held accountable if this is illegal and is found out??This pathologist mind you is the owner of the business. I am afraid if I say something, I will get fired. Where do I go from here or if there is someone who has a similar circumstance happen to them. The other scenario is that this same Pathologist has called a tech a slandering name, twice.? There is no "upper management" to go to since he is the owner of the business. I was wondering if this is considered harassment and can this be used to?nullify a contract signed by both parties for employment. ? Concerned, freckles9660@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ruebenjcarter <@t> gmail.com Sat Jun 14 08:57:25 2008 From: ruebenjcarter <@t> gmail.com (R C) Date: Sat Jun 14 08:57:32 2008 Subject: [Histonet] Tissue Processors Message-ID: <2a926e3f0806140657t9cf9fb3o6f9bb6e5d12ca60e@mail.gmail.com> In my opinion, rotating reagents on your Vip tissue processor can me done once a week when processing 150/night. However, at a 750 block threshold, one might notice tissue microtomy artifact on the 5th day. As in life, trial and error. Your maintenance of service twice a week is safe and proven; I agree with it fully. Ruben Carter, HT ASCP From ruebenjcarter <@t> gmail.com Sat Jun 14 09:05:50 2008 From: ruebenjcarter <@t> gmail.com (R C) Date: Sat Jun 14 09:05:55 2008 Subject: Subject: RE: [Histonet] Logging in same type specimens consecutively Message-ID: <2a926e3f0806140705la3f511eu6310bfc96e9762c6@mail.gmail.com> Subject: RE: [Histonet] Logging in same type specimens consecutively In some laboratories it makes good practice to alternate tissue type in numerical order. In situations of human error, basic human logic can identify a mistake much easier (i.e during embedding, or slide labeling with alternating clored cassettes.) In contrast, a flawless accessioning protocol wouldn't require one to alternate. But who is without flaws? Ruben Carter, HT, ASCP From ruebenjcarter <@t> gmail.com Sat Jun 14 09:09:42 2008 From: ruebenjcarter <@t> gmail.com (R C) Date: Sat Jun 14 09:09:46 2008 Subject: Subject: Re: [Histonet] Weekend differential Message-ID: <2a926e3f0806140709g43b311dcocfe01c014a51fcf0@mail.gmail.com> You mean, high income Pathology labs don't pay additional compensation for histologists to work in the evening (despite the human bodys' sycnhonization with the Sun and productivity) so that patients receive timely results? Ruben Carter, HT ASCP From zodiac29 <@t> comcast.net Sat Jun 14 09:52:08 2008 From: zodiac29 <@t> comcast.net (zodiac29@comcast.net) Date: Sat Jun 14 09:52:19 2008 Subject: Subject: Re: [Histonet] Weekend differential Message-ID: <061420081452.17935.4853DB18000D49C00000460F2216557996C7CD0C0E070B0196@comcast.net> Most of the labs that I know of in my town do. They compensate extra for weekends and nights. -------------- Original message -------------- From: "R C" > You mean, high income Pathology labs don't pay additional compensation for > histologists to work in the evening (despite the human bodys' sycnhonization > with the Sun and productivity) so that patients receive timely results? > > Ruben Carter, HT ASCP > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pathrm35 <@t> comcast.net Sat Jun 14 10:04:55 2008 From: pathrm35 <@t> comcast.net (pathrm35@comcast.net) Date: Sat Jun 14 10:05:01 2008 Subject: Subject: Re: [Histonet] Weekend differential Message-ID: <061420081504.11578.4853DE17000E809900002D3A2215567074CACC039D089B0E9F@comcast.net> Our lab pays 20% diff for second shift and 25% diff for third (and we are currently looking for two techs). -------------- Original message -------------- From: "R C" > You mean, high income Pathology labs don't pay additional compensation for > histologists to work in the evening (despite the human bodys' sycnhonization > with the Sun and productivity) so that patients receive timely results? > > Ruben Carter, HT ASCP > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> pathview.com Sat Jun 14 11:49:06 2008 From: mike <@t> pathview.com (Michael Mihalik) Date: Sat Jun 14 11:49:41 2008 Subject: Subject: RE: [Histonet] Logging in same type specimens consecutively In-Reply-To: <2a926e3f0806140705la3f511eu6310bfc96e9762c6@mail.gmail.com> References: <2a926e3f0806140705la3f511eu6310bfc96e9762c6@mail.gmail.com> Message-ID: I agree that people can and will make mistakes. It can't be avoided. The 'trick' is to design a system of doing things that helps prevent mistakes. ...easy to say, really tough to do. Michael Mihalik PathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of R C Sent: Saturday, June 14, 2008 7:06 AM To: histonet@lists.utsouthwestern.edu Subject: Subject: RE: [Histonet] Logging in same type specimens consecutively Subject: RE: [Histonet] Logging in same type specimens consecutively In some laboratories it makes good practice to alternate tissue type in numerical order. In situations of human error, basic human logic can identify a mistake much easier (i.e during embedding, or slide labeling with alternating clored cassettes.) In contrast, a flawless accessioning protocol wouldn't require one to alternate. But who is without flaws? Ruben Carter, HT, ASCP _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MajorFocus <@t> aol.com Sat Jun 14 18:31:44 2008 From: MajorFocus <@t> aol.com (MajorFocus@aol.com) Date: Sat Jun 14 18:31:53 2008 Subject: [Histonet] help Message-ID: please unsubscribe this email address from your list Kathy M. Good Major Focus Office Manager (800) 888-1152 Phone (828) 396-5567 Fax **************Vote for your city's best dining and nightlife. City's Best 2008. (http://citysbest.aol.com?ncid=aolacg00050000000102) From HDOWNS <@t> PARTNERS.ORG Sat Jun 14 21:11:35 2008 From: HDOWNS <@t> PARTNERS.ORG (Downs, Heather M.) Date: Sat Jun 14 21:15:38 2008 Subject: [Histonet] HM330 microtome parts needed References: Message-ID: You can contact Richard-Allen. They sell the Microm line, as well as service them. Heather MGH Boston Ma ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of histonet-request@lists.utsouthwestern.edu Sent: Sat 6/14/2008 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 55, Issue 24 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Looking for sevice manual again (histocs) 2. HM330 microtome parts needed (histocs) 3. RE: HM330 microtome parts needed (Mickie Johnson) 4. RE: Re: Logging in same type specimens consecutively (Bill) 5. Re: Cleaning Processors (Shelly Coker) 6. accessing messages in archives, the need for authentication (Gayle Callis) 7. RE: Legality of work (Lee & Peggy Wenk) 8. Tissue Processors (R C) 9. Subject: RE: [Histonet] Logging in same type specimens consecutively (R C) 10. Subject: Re: [Histonet] Weekend differential (R C) 11. Re: Subject: Re: [Histonet] Weekend differential (zodiac29@comcast.net) 12. Re: Subject: Re: [Histonet] Weekend differential (pathrm35@comcast.net) 13. RE: Subject: RE: [Histonet] Logging in same type specimens consecutively (Michael Mihalik) ---------------------------------------------------------------------- Message: 1 Date: Fri, 13 Jun 2008 10:49:22 -0700 From: "histocs" Subject: [Histonet] Looking for sevice manual again To: "Histonet" Message-ID: <000c01c8cd7d$d0945fd0$6500a8c0@LHBLION> Content-Type: text/plain; charset="Windows-1252" I am still looking for a copy of the service manual for my TP1050. I have the users manual but I have some minor repairs to make and therefore need to have a service manual. If you would be willing to make a copy I would be happy to pay for it. Contact me at 360-966-7300 or rhbrown1@histocs.com LeRoy Brown HT(ASCP)HTL HCS Everson, wA 98247 ------------------------------ Message: 2 Date: Fri, 13 Jun 2008 11:00:11 -0700 From: "histocs" Subject: [Histonet] HM330 microtome parts needed To: "Histonet" Message-ID: <001301c8cd7f$5375d130$6500a8c0@LHBLION> Content-Type: text/plain; charset="Windows-1252" Does anyone have a Microm, HM330, microtome that might be good for parts? I need the outer plastic housing parts. The one I have was dropped and the plastic shell is broke. Any ideas on who might sell these parts? Many thanks. LeRoy Brown HT(ASCP)HTL HCS 360-966-7300 ------------------------------ Message: 3 Date: Fri, 13 Jun 2008 12:42:11 -0700 From: "Mickie Johnson" Subject: RE: [Histonet] HM330 microtome parts needed To: "'histocs'" , "'Histonet'" Message-ID: Content-Type: text/plain; charset="us-ascii" Dear Leroy, You might try Belair Instrument Co. Their phone number is 600-783-9424. Good Luck! Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histocs Sent: Friday, June 13, 2008 11:00 AM To: Histonet Subject: [Histonet] HM330 microtome parts needed Does anyone have a Microm, HM330, microtome that might be good for parts? I need the outer plastic housing parts. The one I have was dropped and the plastic shell is broke. Any ideas on who might sell these parts? Many thanks. LeRoy Brown HT(ASCP)HTL HCS 360-966-7300 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Fri, 13 Jun 2008 15:19:22 -0500 From: Bill Subject: RE: [Histonet] Re: Logging in same type specimens consecutively To: Message-ID: Content-Type: text/plain; charset="us-ascii" At 11:50 AM -0400 6/13/08, Weems, Joyce wrote: >We make a marker with agar and put a different color in each case. It is >embedded and cut right along with the tissue, so it is a good trail. The >pathologists also document it in the report as a QA measure. Good idea Joyce. Could you provide more info on the technique. How do you color the agar etc. Bill ------------------------------ Message: 5 Date: Fri, 13 Jun 2008 13:47:26 -0700 (PDT) From: Shelly Coker Subject: [Histonet] Re: Cleaning Processors To: histonet@lists.utsouthwestern.edu, Bauer.Karen@mayo.edu Message-ID: <400924.36660.qm@web90302.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I worked in a very large reference lab with the old VIP's, and the gold standard was 300 blocks, and the solutions had to be changed (rotated). That standard would explain why you currently are changing every other day (about 300 blocks). I will say this...when the processors weren't changed as scheduled (occasionally the ball got dropped) we immediately noticed marked difficulty with cutting, and had occasions when the staining was adversely affected as well. We used a hydrometer to check the alcohols as we rotated them, and the amount of carry over surprised me. Occassionally the 100% alcohol would be as low as 95% after the processing run. Had we not changed the alcohols per our protocol, that 5% could be disasterous the next day. Good luck! Shelly ------------------------------ Message: 6 Date: Fri, 13 Jun 2008 16:06:52 -0600 From: "Gayle Callis" Subject: [Histonet] accessing messages in archives, the need for authentication To: "Histonet" Message-ID: <001201c8cda1$c91ed950$6401a8c0@DHXTS541> Content-Type: text/plain; charset="iso-8859-1" Has anyone encountered a window that arises saying one needs to go to "LOGS" at http://www.histosearch.com followed by a username and password to authenticate and access information from the Histonet archive messages. This happened everytime I tried to open the messages with subject: "RE:[Histonet] Re: Logging in same type specimens consecutively" Apparently Histonet archives doesn't like the words logging in????? And this doesn't happen with any other messages in the Archives. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT ------------------------------ Message: 7 Date: Sat, 14 Jun 2008 07:45:08 -0400 From: "Lee & Peggy Wenk" Subject: RE: [Histonet] Legality of work To: "'Karla Arrington'" , Message-ID: <001101c8ce14$18f9c8f0$eff62d4b@HPPav2> Content-Type: text/plain; charset="iso-8859-1" Sorry I didn't get in on this discussion earlier. Have been without electricity since the weekend. I wanted to bring up a couple of other issues. 1. NAACLS Requirements: NAACLS is authorized by the US government to accredit POST-SECONDARY laboratory programs, in other words, after high school. So first of all, to be in a NAACLS program, the person has to have graduated from high school. All the NAACLS programs that have on-line classes would have to have the candidate to be a minimum of a high school graduate. So this 15 year old students wouldn't meet this requirement. Second, under the NAACLS requirement, anyone in a NAACLS program must have had as minimum education, 1 class in each - biology, chemistry and math. This could be in high school, or it could be in college. I doubt that a 15 year old would have had the chemistry requirement, at the very least. 2. NAACLS Programs Requirements: After having said #1, each NAALCS program can set their own standard at or higher than NAACLS standards. So a NAACLS HT program could require that the one biology/chem/math courses be college level, or could say that the biology class must be college anatomy and physiology. Or that the person must have an associate degree. (Just to let you know, my program (not on-line) requires an associate degree, anatomy, physiology, microbiology, 2 chemistry, and intermediate college algebra as minimum requirements.) So, if anyone is interested in any of the HT on-line programs, the candidate would have to meet not only the NAACLS minimum education requirements but also the Program's minimum education requirements. This student would not. 3. NAACLS vs OJT routes of entry: Just a reminder, in order to take the HT exam, this student either has to complete a NAACLS HT program (which as of right now she can't get into because of not having the high school diploma and not having all the bio/chem/math classes), nor can she take the HT exam via the on-the-job (OJT) route, as she does not have an associate degree with 12 credits of biology and chemistry. 4. Appearance, Legally: One last thing, which I may get blasted for. Yes, I love the idea of job shadowing, and think it is great in terms of students working with already diagnosed tissue that was going to be disposed of, to set up their own study sets/portfolios. However, having a student work on real patient tissue that is involved in the diagnosis - well, let's look at it from a lawyer's point of view. Let's say this 15 year old high school student makes a mistake - embeds a skin wrong, cuts through the tissue, makes up a stain incorrectly. We've all done it, I know, but what would the lawyer for the patient say to a jury about this situation? That is pathologist hired a underage high school student -(that according to the originator of this chain) it is illegal for a 15 year old to be in a lab working with chemical and BBP. - This student is not eligible to get into a NAACLS HT program - not a high school graduate (or higher) and without the correct bio/chem/math requirements. - This student is not eligible to take the ASCP registry exam via the OJT route - no associate degree with 12 credits of bio/chem This lawyer is going to tell the jury that this lab hired a student illegally and who could not meet the national standards for training or taking a national certification exam. And that the lab and director did this knowingly. (after all, the regulations and standards are available, and trust me, a lawyer would google this). So what do you think a jury would do? What would their verdict be? Would it go for or against the lab and the director? Remember, this is the JURY'S PERCEPTION (not what we as histotechs think/know). Personally, I think the verdict would go against the lab and director, and let's hike up the amount of money the director/lab has to pay the patient and their family. So I might recommend that anyone involved in a situation like this - purchase malpractice insurance - techs included. Peggy A. Wenk, HTL(ASCP)SLS Schools of Histotechnology (which is NAACLS accredited - my disclaimer) William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Karla Arrington Sent: Wednesday, June 04, 2008 7:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Legality of work Histo's: I have roughly 2 dilemmas. The first is of a legal matter. The Pathologist for a week has had his daughter (15 years old), helping me out in the histology lab. He wants her to get training from me, then do the on-line HT program. She has handled chemicals and reagents. I am very uncomfortable with this. I have called the Child Labor Laws department for our state and it is illegal for a 15 year to be in a laboratory. Needless to say working with blood borne pathogens. My most concern is the following. As her "teacher", can I or other co-workers can be held accountable if this is illegal and is found out? This pathologist mind you is the owner of the business. I am afraid if I say something, I will get fired. Where do I go from here or if there is someone who has a similar circumstance happen to them. The other scenario is that this same Pathologist has called a tech a slandering name, twice. There is no "upper management" to go to since he is the owner of the business. I was wondering if this is considered harassment and can this be used to nullify a contract signed by both parties for employment. Concerned, freckles9660@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Sat, 14 Jun 2008 06:57:25 -0700 From: "R C" Subject: [Histonet] Tissue Processors To: histonet@lists.utsouthwestern.edu Message-ID: <2a926e3f0806140657t9cf9fb3o6f9bb6e5d12ca60e@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 In my opinion, rotating reagents on your Vip tissue processor can me done once a week when processing 150/night. However, at a 750 block threshold, one might notice tissue microtomy artifact on the 5th day. As in life, trial and error. Your maintenance of service twice a week is safe and proven; I agree with it fully. Ruben Carter, HT ASCP ------------------------------ Message: 9 Date: Sat, 14 Jun 2008 07:05:50 -0700 From: "R C" Subject: Subject: RE: [Histonet] Logging in same type specimens consecutively To: histonet@lists.utsouthwestern.edu Message-ID: <2a926e3f0806140705la3f511eu6310bfc96e9762c6@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Subject: RE: [Histonet] Logging in same type specimens consecutively In some laboratories it makes good practice to alternate tissue type in numerical order. In situations of human error, basic human logic can identify a mistake much easier (i.e during embedding, or slide labeling with alternating clored cassettes.) In contrast, a flawless accessioning protocol wouldn't require one to alternate. But who is without flaws? Ruben Carter, HT, ASCP ------------------------------ Message: 10 Date: Sat, 14 Jun 2008 07:09:42 -0700 From: "R C" Subject: Subject: Re: [Histonet] Weekend differential To: histonet@lists.utsouthwestern.edu Message-ID: <2a926e3f0806140709g43b311dcocfe01c014a51fcf0@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 You mean, high income Pathology labs don't pay additional compensation for histologists to work in the evening (despite the human bodys' sycnhonization with the Sun and productivity) so that patients receive timely results? Ruben Carter, HT ASCP ------------------------------ Message: 11 Date: Sat, 14 Jun 2008 14:52:08 +0000 From: zodiac29@comcast.net Subject: Re: Subject: Re: [Histonet] Weekend differential To: histonet@lists.utsouthwestern.edu Message-ID: <061420081452.17935.4853DB18000D49C00000460F2216557996C7CD0C0E070B0196@comcast.n et> Content-Type: text/plain Most of the labs that I know of in my town do. They compensate extra for weekends and nights. -------------- Original message -------------- From: "R C" > You mean, high income Pathology labs don't pay additional compensation for > histologists to work in the evening (despite the human bodys' sycnhonization > with the Sun and productivity) so that patients receive timely results? > > Ruben Carter, HT ASCP > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Sat, 14 Jun 2008 15:04:55 +0000 From: pathrm35@comcast.net Subject: Re: Subject: Re: [Histonet] Weekend differential To: "R C" Cc: histonet@lists.utsouthwestern.edu Message-ID: <061420081504.11578.4853DE17000E809900002D3A2215567074CACC039D089B0E9F@comcast.n et> Content-Type: text/plain Our lab pays 20% diff for second shift and 25% diff for third (and we are currently looking for two techs). -------------- Original message -------------- From: "R C" > You mean, high income Pathology labs don't pay additional compensation for > histologists to work in the evening (despite the human bodys' sycnhonization > with the Sun and productivity) so that patients receive timely results? > > Ruben Carter, HT ASCP > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Sat, 14 Jun 2008 09:49:06 -0700 From: "Michael Mihalik" Subject: RE: Subject: RE: [Histonet] Logging in same type specimens consecutively To: "'R C'" , Message-ID: Content-Type: text/plain; charset="us-ascii" I agree that people can and will make mistakes. It can't be avoided. The 'trick' is to design a system of doing things that helps prevent mistakes. ..easy to say, really tough to do. Michael Mihalik PathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of R C Sent: Saturday, June 14, 2008 7:06 AM To: histonet@lists.utsouthwestern.edu Subject: Subject: RE: [Histonet] Logging in same type specimens consecutively Subject: RE: [Histonet] Logging in same type specimens consecutively In some laboratories it makes good practice to alternate tissue type in numerical order. In situations of human error, basic human logic can identify a mistake much easier (i.e during embedding, or slide labeling with alternating clored cassettes.) In contrast, a flawless accessioning protocol wouldn't require one to alternate. But who is without flaws? Ruben Carter, HT, ASCP _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 55, Issue 24 **************************************** The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From laurie <@t> conxis.com Sun Jun 15 08:56:40 2008 From: laurie <@t> conxis.com (Laurie Popp) Date: Sun Jun 15 08:56:37 2008 Subject: [Histonet] re: shift and weekend differential Message-ID: <48551F98.80201@conxis.com> Mayo does. I believe it was shift dependent as far as how much the differential was and then the differential had hours that you had to be punched in by in order for it to take effect. They also do on call so we get paid a different rate for on call depending on whether we get called in or not. Laurie Popp HT ( ASCP) cm Mayo clinic From AnthonyH <@t> chw.edu.au Sun Jun 15 18:15:50 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Jun 15 18:16:04 2008 Subject: [Histonet] Logging in same type specimens consecutively In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82CBD@EMAIL.archildrens.org> Message-ID: I suppose one should try if possible. If there is a policy in the methods manual recommending that like-specimens and specimens from patients with same or similar names not be accessioned consecutively and the policy is shown to be followed as much as possible, then that is the best practice that is achievable. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Friday, 13 June 2008 11:38 PM To: Della Speranza, Vinnie; Barbara Rouse; Histonet Subject: RE: [Histonet] Logging in same type specimens consecutively I don't understand why it is considered poor practice to accession like specimens. Every part of the clinical lab does this every day. We get so many biopsies of the same kind we cannot accession them without accessioning them consecutively. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Thursday, June 12, 2008 10:20 AM To: 'Barbara Rouse'; Histonet Subject: RE: [Histonet] Logging in same type specimens consecutively The practice you are describing is recommended in the AP Quality Assurance Manual published by the CAP. It is considered poor practice to accession like specimens from different patients consecutively. The reference is Nakhleh RE, Fitzgibbons PL. Quality improvement manual in anatomic pathology, second edition. Northfield, IL: CAP; 2002 I am not aware that this is a checklist item in the accreditation requirements for AP Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Rouse Sent: Wednesday, June 11, 2008 8:41 PM To: Histonet Subject: [Histonet] Logging in same type specimens consecutively Does anyone know of a regulation by CAP that states that same type specimens should not be logged in consecutively? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ****************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Sun Jun 15 18:49:34 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Jun 15 18:49:40 2008 Subject: [Histonet] Tissue Processors In-Reply-To: <3C25F9EF8E8DF84DBBB1884C297E8AF40379079F@ex03.fmchealth.org> Message-ID: We use microwave processing for urgent biopsies, eg liver and renal biopsies. Our pathologists actually prefer microwave processed renal biopsies over the routine processing. We use an extended formalin fixation step, followed by a rinse in 70% ethanol, microwave in isopropanol, rinse in melted wax (to remove excess isopropanol), followed by microwave in wax. We let specimens rest in the wax (in the embedding centre) for 5-10 minutes prior to embedding. This is done on the Milestone T/T Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edie Lehman Sent: Saturday, 14 June 2008 12:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Processors First many thanks to all who responded to my request for autostainer information. It has been most helpful. Second, could I please get some input on Tissue Processors? Anyone using the microwave technology, and if so, how do you like it? Do the pathologist like it? Again any advice is appreciated. Thanks in advance, Edie Lehman MT(ASCP) Anatomical Pathology Supervisor Fairfield Medical Center EdieL@fmchealth.org (740)687-8807 "Confidentiality Notice: This e-mail message, including any attachments is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review; use; disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From mhanna <@t> histosearch.com Sun Jun 15 23:58:30 2008 From: mhanna <@t> histosearch.com (Marvin Hanna) Date: Sun Jun 15 23:58:37 2008 Subject: [Histonet] Re: accessing messages in archives, the need for authentication In-Reply-To: <001201c8cda1$c91ed950$6401a8c0@DHXTS541> References: <001201c8cda1$c91ed950$6401a8c0@DHXTS541> Message-ID: Hi Gayle, Actually, the problem was the server got hit by lightning Friday which caused this issue. It has been corrected and all links are working properly now. Thanks for notifying me about this. Kind Regards, Marvin On Jun 13, 2008, at 5:06 PM, Gayle Callis wrote: > Has anyone encountered a window that arises saying one needs to go > to "LOGS" at http://www.histosearch.com followed by a username and > password to authenticate and access information from the Histonet > archive messages. This happened everytime I tried to open the > messages with subject: > > > "RE:[Histonet] Re: Logging in same type specimens consecutively" > > Apparently Histonet archives doesn't like the words logging in????? > And this doesn't happen with any other messages in the Archives. > > Gayle M. Callis > HTL/HT/MT(ASCP) > Bozeman MT > > From relia1 <@t> earthlink.net Mon Jun 16 07:13:32 2008 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Jun 16 07:13:39 2008 Subject: [Histonet] RELIA Special Job Alert. 06/16/08 Not your everday histology job!! Message-ID: Hi Histonetters. I hope everybody had a great weekend!! I have an exciting opportunity with a premier client located in the Chicago area with in their Technical Assistance Center (TAC) for an Applications Specialist that will provide technical phone support and product training on my client?s routine histology and immunohistochemistry product lines. The position is based in the Chicago area office?s new Customer Support Laboratory. Qualified applicants should possess a Bachelor of Science degree or related field and a minimum of two years histology, immunohistochemistry and/or ultramicrotomy experience. Candidates should possess excellent communication, teambuilding and organizational skills, Microsoft Office proficiency and excellent customer relations skills. My client offers great benefits (including medical, dental, vision, prescription, long term care, life insurance, STD, LTD, 401K and a retirement savings plan), excellent salary, vacation and holiday schedule. If you are interested please contact me. I can be reached at relia1@earthlink.net or toll free at 866-607-3542. If you know someone who might be interested please feel free to pass the information along to them as well. Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.myspace.com/pamatrelia From danand24 <@t> gmail.com Mon Jun 16 10:43:39 2008 From: danand24 <@t> gmail.com (Anand D) Date: Mon Jun 16 10:43:46 2008 Subject: [Histonet] Trouble with DPBS Message-ID: <30281590806160843v1f11999r75690efcbde11092@mail.gmail.com> Dear Friends. I have a problem retrieving my frozen sections that is stored in DPBS. Instead of storing in PBS mistakenly it is stored in DPBS and whenever i am trying to pick up the section from the multi well plate the sections get adhere to each other or within itself or with the brush and form a tissue clump. Once they formed as a clump i can not able to spread them strighte and use it for immunocytochemistry.These tissues are very very valuable for my study and have be waited for 6 months to get these tissue. They are all mouse brain section of 30 micron thick. . Can anyone help me in suggesting possiblites to retrieve these sections. Your help will be highly appreciated. Thank you. Andy From sbreeden <@t> nmda.nmsu.edu Mon Jun 16 10:48:42 2008 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Mon Jun 16 10:48:51 2008 Subject: [Histonet] New Mexico NSH Meeting Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E63A6@nmdamailsvr.nmda.ad.nmsu.edu> To all of you who are headed this direction for the NSH Summer Symposium on Friday and Saturday - just a reminder! This is SUMMER and this is NEW MEXICO. It's hot and it's DRY (95-ish degrees this week and about 10%-ish humidity). Mornings and evenings are usually (of course, this jinxes it, you know...) cool at about 60 degrees and the evenings after sunset are warm and wonderful for seeing the sights. Bring lotion and remember to drink lots of liquids (the choice of liquid is entirely up to you... "what happens in Albuquerque stays in Albuquerque"!!). Oh, yeah - you don't need a passport to get into the State. See you Friday!! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From histo20 <@t> hotmail.com Mon Jun 16 11:13:19 2008 From: histo20 <@t> hotmail.com (Paula Wilder) Date: Mon Jun 16 11:13:29 2008 Subject: [Histonet] uneven HE staining Message-ID: Hi everyone! A colleague of mine is having uneven staining problems with their automatic stainer, which I believe is a Tissue-Tek stainer. This has been an ongoing problem. She tells me it is only on biopsies, very intermittent. On one slide she might have four sections; three stain evenly and one does not. The location of the unevenly stained section is not always the same; sometimes it is the first section, sometimes the last. Several technicians are cutting so technique has been eliminated. They tried tracking the biopsies to see if a particular doctor's office was involved, but again, no. Does anyone have a clue or suggestion which direction should be pursued next? We don't think it is the processing; dehydration? I do not have their HE programmed times. Thanks for your help, Paula Wilder _________________________________________________________________ Instantly invite friends from Facebook and other social networks to join you on Windows Live? Messenger. https://www.invite2messenger.net/im/?source=TXT_EML_WLH_InviteFriends From JCollins <@t> palmbeachpath.com Mon Jun 16 11:39:01 2008 From: JCollins <@t> palmbeachpath.com (Judy Collins) Date: Mon Jun 16 11:39:08 2008 Subject: [Histonet] uneven HE staining In-Reply-To: References: Message-ID: <05CAE76AB5D5ED409864C6DD86F1334901D4413495@pbpsflexch02.pbp.local> The way it is described sounds like thick and thin sections, a cutting problem, not a staining problem. Judy Collins -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Wilder Sent: Monday, June 16, 2008 12:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] uneven HE staining Hi everyone! A colleague of mine is having uneven staining problems with their automatic stainer, which I believe is a Tissue-Tek stainer. This has been an ongoing problem. She tells me it is only on biopsies, very intermittent. On one slide she might have four sections; three stain evenly and one does not. The location of the unevenly stained section is not always the same; sometimes it is the first section, sometimes the last. Several technicians are cutting so technique has been eliminated. They tried tracking the biopsies to see if a particular doctor's office was involved, but again, no. Does anyone have a clue or suggestion which direction should be pursued next? We don't think it is the processing; dehydration? I do not have their HE programmed times. Thanks for your help, Paula Wilder _________________________________________________________________ Instantly invite friends from Facebook and other social networks to join you on Windows Live(tm) Messenger. https://www.invite2messenger.net/im/?source=TXT_EML_WLH_InviteFriends_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From danand24 <@t> gmail.com Mon Jun 16 11:41:49 2008 From: danand24 <@t> gmail.com (Anand D) Date: Mon Jun 16 11:41:58 2008 Subject: [Histonet] Trouble retrieving section from DPBS buffer Message-ID: <30281590806160941p1f4022a6o41085ebf36b1d09a@mail.gmail.com> Dear Friends. I have a problem retrieving my frozen sections that is stored in DPBS. Instead of storing in PBS mistakenly it is stored in DPBS and whenever i am trying to pick up the section from the multi well plate the sections get adhere to each other or within itself or with the brush and form a tissue clump. Once they formed as a clump i can not able to spread them strighte and use it for immunocytochemistry.These tissues are very very valuable for my study and have be waited for 6 months to get these tissue. They are all mouse brain section of 30 micron thick. . Can anyone help me in suggesting possiblites to retrieve these sections. Your help will be highly appreciated. Thank you. Andy From rjbuesa <@t> yahoo.com Mon Jun 16 12:04:50 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jun 16 12:19:01 2008 Subject: [Histonet] uneven HE staining In-Reply-To: Message-ID: <947689.37209.qm@web65716.mail.ac4.yahoo.com> Usually uneven staining is due to incomplete dewaxing. Tell your colleague to check the xylene (or whatever dewaxing agent she is using) and make sure that all the sections are within that level. Ren? J. Paula Wilder wrote: Hi everyone! A colleague of mine is having uneven staining problems with their automatic stainer, which I believe is a Tissue-Tek stainer. This has been an ongoing problem. She tells me it is only on biopsies, very intermittent. On one slide she might have four sections; three stain evenly and one does not. The location of the unevenly stained section is not always the same; sometimes it is the first section, sometimes the last. Several technicians are cutting so technique has been eliminated. They tried tracking the biopsies to see if a particular doctor's office was involved, but again, no. Does anyone have a clue or suggestion which direction should be pursued next? We don't think it is the processing; dehydration? I do not have their HE programmed times. Thanks for your help, Paula Wilder _________________________________________________________________ Instantly invite friends from Facebook and other social networks to join you on Windows Live? Messenger. https://www.invite2messenger.net/im/?source=TXT_EML_WLH_InviteFriends_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MLinville <@t> ameripath.com Mon Jun 16 12:48:46 2008 From: MLinville <@t> ameripath.com (Linville, Marsha) Date: Mon Jun 16 12:48:51 2008 Subject: FW: [Histonet] uneven HE staining Message-ID: <8613338BCE81D0438B9823A8AF6BEE2D04CC9228@TXCLMAIL01.ameripath.local> Hi, Paula, Two different things you might look at. First, do you use Sta-On in your waterbath? I have had a similar problem in the past because of it, very random. Oddly enough, we really only noticed it on prostate core biopsies. Problem disappeared immediately when we quit using the Sta-On. Second, are you using SurgiPath charged slides? I have seen them produce a similar problem in IHC's, apparently due to the fact that their charge isn't always even across the slides and some areas become hydrophobic. If it is either of these, happily the fix is very easy. Marsha Linville Histology Supervisor AmeriPath Indiana Phone: (317)275-8140 Fax: (317)275-8070 Pager: (317)568-5407 -----Original Message----- From: Paula Wilder [mailto:histo20@hotmail.com] Sent: Monday, June 16, 2008 12:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] uneven HE staining Hi everyone! A colleague of mine is having uneven staining problems with their automatic stainer, which I believe is a Tissue-Tek stainer. This has been an ongoing problem. She tells me it is only on biopsies, very intermittent. On one slide she might have four sections; three stain evenly and one does not. The location of the unevenly stained section is not always the same; sometimes it is the first section, sometimes the last. Several technicians are cutting so technique has been eliminated. They tried tracking the biopsies to see if a particular doctor's office was involved, but again, no. Does anyone have a clue or suggestion which direction should be pursued next? We don't think it is the processing; dehydration? I do not have their HE programmed times. Thanks for your help, Paula Wilder _________________________________________________________________ Instantly invite friends from Facebook and other social networks to join you on Windows Live(tm) Messenger. https://www.invite2messenger.net/im/?source=TXT_EML_WLH_InviteFriends From PMonfils <@t> Lifespan.org Mon Jun 16 12:50:46 2008 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Mon Jun 16 12:50:50 2008 Subject: [Histonet] tannic acid-crystal violet method? Victoria blue? Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835C02@LSRIEXCH1.lsmaster.lifespan.org> Hi all. I'm still looking for a good method for staining cell boundaries in paraffin sections. I found a reference to a tannic acid-crystal violet procedure (Robinow & Murray 1953) but cannot find the actual protocol anywhere. There is also a refence to a Victoria blue technique. If anyone has a protocol for either of these procedures, I would appreciate it. Thanks. From gentras <@t> vetmed.auburn.edu Mon Jun 16 14:06:31 2008 From: gentras <@t> vetmed.auburn.edu (Atoska Gentry) Date: Mon Jun 16 14:06:41 2008 Subject: [Histonet] uneven HE staining In-Reply-To: References: Message-ID: <4856B9B7.70405@vetmed.auburn.edu> Hello, when this type staining problem occurred in manual staining it was discovered to be caused by incomplete deparaffinization. Corrective measure taken was to replace clearing agents with fresh solution(s). However, I'm not familiar with the Tissue Tek Stainer or any of the other automated stainers. Best wishes. Atoska Paula Wilder wrote: > Hi everyone! > > A colleague of mine is having uneven staining problems with their automatic stainer, which I believe is a Tissue-Tek stainer. This has been an ongoing problem. She tells me it is only on biopsies, very intermittent. On one slide she might have four sections; three stain evenly and one does not. The location of the unevenly stained section is not always the same; sometimes it is the first section, sometimes the last. Several technicians are cutting so technique has been eliminated. They tried tracking the biopsies to see if a particular doctor's office was involved, but again, no. Does anyone have a clue or suggestion which direction should be pursued next? We don't think it is the processing; dehydration? I do not have their HE programmed times. > > Thanks for your help, > Paula Wilder > _________________________________________________________________ > Instantly invite friends from Facebook and other social networks to join you on Windows Live? Messenger. > https://www.invite2messenger.net/im/?source=TXT_EML_WLH_InviteFriends_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lelmgren <@t> sunriselab.com Mon Jun 16 14:43:49 2008 From: lelmgren <@t> sunriselab.com (Laurie Elmgren) Date: Mon Jun 16 14:43:57 2008 Subject: [Histonet] uneven HE staining In-Reply-To: <4856B9B7.70405@vetmed.auburn.edu> References: <4856B9B7.70405@vetmed.auburn.edu> Message-ID: <4A672C6AE0402D4A89ECE29E8A4B47E3010F481A@MailPDC.sunriselab.com> Hi Paula and Atoska, We have had the same problem from time to time. We have a Sakura DRS 200. I tend to think that the water was not fully drained from the slides before racking them. Our procedure is to stand the slides up vertically against the waterbath on paper toweling for at least 30 seconds before racking them. The water that remains under the tissue, steams it in the oven, effecting the ability of the sections to take up stain. We also change all of the solutions after every shift, and the xylene and alcohol after ten full runs of slides. Laurie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Atoska Gentry Sent: Monday, June 16, 2008 3:07 PM To: Paula Wilder; Histonet Subject: Re: [Histonet] uneven HE staining Hello, when this type staining problem occurred in manual staining it was discovered to be caused by incomplete deparaffinization. Corrective measure taken was to replace clearing agents with fresh solution(s). However, I'm not familiar with the Tissue Tek Stainer or any of the other automated stainers. Best wishes. Atoska Paula Wilder wrote: > Hi everyone! > > A colleague of mine is having uneven staining problems with their automatic stainer, which I believe is a Tissue-Tek stainer. This has been an ongoing problem. She tells me it is only on biopsies, very intermittent. On one slide she might have four sections; three stain evenly and one does not. The location of the unevenly stained section is not always the same; sometimes it is the first section, sometimes the last. Several technicians are cutting so technique has been eliminated. They tried tracking the biopsies to see if a particular doctor's office was involved, but again, no. Does anyone have a clue or suggestion which direction should be pursued next? We don't think it is the processing; dehydration? I do not have their HE programmed times. > > Thanks for your help, > Paula Wilder > _________________________________________________________________ > Instantly invite friends from Facebook and other social networks to join you on Windows Live(tm) Messenger. > https://www.invite2messenger.net/im/?source=TXT_EML_WLH_InviteFriends___ ____________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Robinsoc <@t> mercyhealth.com Mon Jun 16 15:23:23 2008 From: Robinsoc <@t> mercyhealth.com (Cynthia Robinson) Date: Mon Jun 16 15:23:46 2008 Subject: [Histonet] fixation documention for her2-neu Message-ID: <4856856B.59BC.00AF.0@mercyhealth.com> We are having quite a time deciding how we are going to handle documentation concerning fixation for specimens requiring Her2-neu IHC testing and interpretation. What is the actual fixation start time? Is it when the large specimen first has 10% formalin added or when the specimen is actually grossed and sections are taken? The CAP guidelines were discussed in Denver at the NSH meeting and I understood that the start time was when the specimen was cut open or 'breadloafed' or when it was grossed in. However, this is not stated very clearly by CAP. What are you doing out there in Histoland? Are you using a general statement on your reports to reflect processing within 6-48 hrs fixation, < 6-48 hrs fixation or > 48 hr fixation or are you actually documenting times when formalin was added, gross time, processor start and end times? Please comment as I need to give my supervisor and the pathologists other perspectives on this controversial issue. Thanks. Cindi Robinson HT(ASCP) Mercy-Sioux City 801 Fifth St Sioux City IA 51101 Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 From AGrobe2555 <@t> aol.com Mon Jun 16 15:38:07 2008 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Mon Jun 16 15:36:15 2008 Subject: [Histonet] Entellan, DePeX and IF Message-ID: Good afternoon, A question for those of you that do immunofluorescence: Have you used Entellan or DePeX as a coverslipping medium for IF (after dehydration)? How well do they work? Do they preventing fading, bleaching, etc? Thanks, Albert Albert C. Grobe, PhD Tissue Engineering Lab International Heart Institute of Montana Foundation **************Gas prices getting you down? Search AOL Autos for fuel-efficient used cars. (http://autos.aol.com/used?ncid=aolaut00050000000007) From bdelescavage <@t> cellnetix.com Mon Jun 16 15:55:53 2008 From: bdelescavage <@t> cellnetix.com (Beth Delescavage) Date: Mon Jun 16 15:56:49 2008 Subject: [Histonet] VIP Processor Manuals Message-ID: Hi Everyone~ We are looking for VIP operation manuals for the following processors, 1. VIP 5 2. VIP 3000 3. VIP E300 Hard or digital copies would be greatly appreciated. Please contact me with any questions or concerns. Thanks~ Beth Beth Delescavage, BS, HTL (ASCP) QIHC CellNetix Laboratories DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From will <@t> histologytechservices.com Mon Jun 16 16:13:16 2008 From: will <@t> histologytechservices.com (William Connor) Date: Mon Jun 16 16:13:34 2008 Subject: [Histonet] NH4Cl Message-ID: <0MKp8S-1K8M131pdX-0003c4@mrelay.perfora.net> Greetings, Could anyone tell me why a pretreatment of 50 mM NH4Cl would be used in immunohistochemistry? Thanks. William P. Connor, HT(ASCP) Senior Histology Technician Histology Tech Services, Inc. This email and its attachments (if any) contain confidential and privileged information. Any dissemination or use of this information by a person other than the intended recipient is unauthorized and may be illegal. From Rcartun <@t> harthosp.org Mon Jun 16 17:31:12 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Mon Jun 16 17:31:23 2008 Subject: [Histonet] fixation documention for her2-neu In-Reply-To: <4856856B.59BC.00AF.0@mercyhealth.com> References: <4856856B.59BC.00AF.0@mercyhealth.com> Message-ID: <4856B17002000077000037F1@gwmail6.harthosp.org> I've asked our breast surgeons and interventional radiologists to put the time that the specimen goes into the formalin container (in their office or in radiology) on the pathology requisition. Pathology personnel (residents, fellows, PAs, and attendings) are responsible for noting the time of "formalin contact" on the pathology requisition for excision specimens received from our OR. We are in the process of instituting a 3:30 pm cut-off time for all "large" specimens. Our tissue processors start at 5:30 pm and have 4 hours of formalin on them. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Cynthia Robinson" 06/16/08 4:23 PM >>> We are having quite a time deciding how we are going to handle documentation concerning fixation for specimens requiring Her2-neu IHC testing and interpretation. What is the actual fixation start time? Is it when the large specimen first has 10% formalin added or when the specimen is actually grossed and sections are taken? The CAP guidelines were discussed in Denver at the NSH meeting and I understood that the start time was when the specimen was cut open or 'breadloafed' or when it was grossed in. However, this is not stated very clearly by CAP. What are you doing out there in Histoland? Are you using a general statement on your reports to reflect processing within 6-48 hrs fixation, < 6-48 hrs fixation or > 48 hr fixation or are you actually documenting times when formalin was added, gross time, processor start and end times? Please comment as I need to give my supervisor and the pathologists other perspectives on this controversial issue. Thanks. Cindi Robinson HT(ASCP) Mercy-Sioux City 801 Fifth St Sioux City IA 51101 Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hilda_1075 <@t> yahoo.com Mon Jun 16 22:22:16 2008 From: hilda_1075 <@t> yahoo.com (shazana hilda) Date: Mon Jun 16 22:22:19 2008 Subject: [Histonet] positive control for PPARgamma, PKCalpha,Flk-1and eNOS Message-ID: <112487.44087.qm@web38805.mail.mud.yahoo.com> Dear All, I'm having problem to determine which positive controls are suitable to these marker for immunohistochemistry (IHC):PPARgamma,Flk-1, PKCalpha and eNOS. I've already follow the antibody kit instruction provided by Santacruz Biotechnology Inc. and repeated 5x.but still cannot produce result. these is the list of positive control for each antibody: 1)PPARgamma (sc-7273): Urothelial Carcinoma and Invasive Ductal Carcinoma (NOS) 2) Flk-1(sc-6251): Colon Carcinoma 3) PKCalpha(sc-8393): Colon Carcinoma 4) eNOS(sc-654): Serous Carcinoma@Ovary Carcinoma Your kind response are very much appreciated! Many Thanks, Shazana Hilda Shamsuddin MSc.of Molecular Pathology, Dept. of Pathology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia. Phone no: +6013-921 2002 Off. no: +609- 766 4440 Fax no: +609- 765 3370 Email: hilda_1075@yahoo.com From rjbuesa <@t> yahoo.com Tue Jun 17 06:52:56 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jun 17 06:53:00 2008 Subject: [Histonet] NH4Cl In-Reply-To: <0MKp8S-1K8M131pdX-0003c4@mrelay.perfora.net> Message-ID: <550583.23216.qm@web65706.mail.ac4.yahoo.com> Never heard of such a pretreatment with routine IHC protocols. Ren? J. William Connor wrote: Greetings, Could anyone tell me why a pretreatment of 50 mM NH4Cl would be used in immunohistochemistry? Thanks. William P. Connor, HT(ASCP) Senior Histology Technician Histology Tech Services, Inc. This email and its attachments (if any) contain confidential and privileged information. Any dissemination or use of this information by a person other than the intended recipient is unauthorized and may be illegal. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hstevens3 <@t> mail.gatech.edu Tue Jun 17 07:11:15 2008 From: hstevens3 <@t> mail.gatech.edu (Hazel Stevens) Date: Tue Jun 17 07:11:31 2008 Subject: [Histonet] NH4Cl In-Reply-To: <550583.23216.qm@web65706.mail.ac4.yahoo.com> References: <0MKp8S-1K8M131pdX-0003c4@mrelay.perfora.net> <550583.23216.qm@web65706.mail.ac4.yahoo.com> Message-ID: <001a01c8d073$3ddd9f50$b998ddf0$@gatech.edu> It is used to quench autofluorescence of the tissue. Hazel -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, June 17, 2008 7:53 AM To: William Connor; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] NH4Cl Never heard of such a pretreatment with routine IHC protocols. Ren? J. William Connor wrote: Greetings, Could anyone tell me why a pretreatment of 50 mM NH4Cl would be used in immunohistochemistry? Thanks. William P. Connor, HT(ASCP) Senior Histology Technician Histology Tech Services, Inc. This email and its attachments (if any) contain confidential and privileged information. Any dissemination or use of this information by a person other than the intended recipient is unauthorized and may be illegal. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Jun 17 08:06:15 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jun 17 08:06:19 2008 Subject: [Histonet] NH4Cl In-Reply-To: <001a01c8d073$3ddd9f50$b998ddf0$@gatech.edu> Message-ID: <297056.58615.qm@web65714.mail.ac4.yahoo.com> Thanks, now I know! Ren? J. Hazel Stevens wrote: It is used to quench autofluorescence of the tissue. Hazel -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, June 17, 2008 7:53 AM To: William Connor; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] NH4Cl Never heard of such a pretreatment with routine IHC protocols. Ren? J. William Connor wrote: Greetings, Could anyone tell me why a pretreatment of 50 mM NH4Cl would be used in immunohistochemistry? Thanks. William P. Connor, HT(ASCP) Senior Histology Technician Histology Tech Services, Inc. This email and its attachments (if any) contain confidential and privileged information. Any dissemination or use of this information by a person other than the intended recipient is unauthorized and may be illegal. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asachau <@t> titanmed.com Tue Jun 17 11:04:08 2008 From: asachau <@t> titanmed.com (April Sachau) Date: Tue Jun 17 11:07:51 2008 Subject: [Histonet] Long Term Histology position In-Reply-To: Message-ID: <7E3ACD48BA6E26408F3188FBF08693F701539061@titansbs1.corp.titanmed.com> Hello, I have a 9 month assignment available for routine Histology available in the Midwest. Dayshift (7a - 330p), Monday through Friday - NO weekends NO on call! Please contact me to discuss the GREAT compensation package. I look forward to hearing from anyone interested. We also offer referral bonuses if you know of anyone you might think would be great for the job! April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com From cmiller <@t> physlab.com Tue Jun 17 11:37:44 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Tue Jun 17 11:36:23 2008 Subject: [Histonet] FW: Anatomic computer systems Message-ID: <000001c8d098$77f88040$3d02a8c0@plab.local> Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 _____ From: Cheri Miller [mailto:cmiller@physlab.com] Sent: Tuesday, June 17, 2008 10:34 AM To: histonet-bounces@lists.utsouthwestern.edu Subject: Anatomic computer systems Time to come out of the dark ages, finally we are getting a computer system for Anatomic path. The powers that be want to have the clinical processing department process our specimens. I need some support here in trying to convince them that clinical and anatomic path are like comparing apples to bananas. My experience with this has always been that the gross room/PA's would data enter and process histology specimens. What do you do?? Who enters, processes and sets up your surgicals? Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From LSebree <@t> uwhealth.org Tue Jun 17 11:41:46 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Tue Jun 17 11:41:56 2008 Subject: [Histonet] FW: Anatomic computer systems In-Reply-To: <000001c8d098$77f88040$3d02a8c0@plab.local> Message-ID: Our grossing room receives, accessions, grosses and sets up the surgical specimens. Our PA and pathology residents do this. We use Tamtron's PowerPath LIS. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 From abehan <@t> rcsi.ie Tue Jun 17 11:42:31 2008 From: abehan <@t> rcsi.ie (Aine Behan) Date: Tue Jun 17 11:43:09 2008 Subject: [Histonet] (no subject) Message-ID: <3DBACD0C44A1CF429431C0C6DC3E81690316D892@crc_exchange.rcsi-internal.ie> I was looking for advice on troubleshooting already cryoprotected tissue for freezing artefact (FA). I have mice brains dissected out after cervical dislocation, postfixed in 4% Paraformaldehyde/PBS overnight (4 degrees), cryoprotected in 30% sucrose for ~24 hours (4 degrees) and then placed in -80 freezer. This FA never was an issue before and the animals are treatment free so anyone know why I would be getting this now? Regards, ?ine From Bauer.Karen <@t> mayo.edu Tue Jun 17 11:56:23 2008 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen) Date: Tue Jun 17 11:56:40 2008 Subject: [Histonet] Black artifact on slides Message-ID: Has anyone encountered black, clumpy "stuff" on slides from using a slide etcher? It could be compared to graphite particles from writing on the slides with pencil, but we do not use pencil anywhere. I'm thinking it could be the dust from the etcher and I'm wondering if anyone else has dealt with this. The pathologists are seeing these particles on our IP slides and Surgical H&E's. Since they are stained by two separate stainers, the only things in common are the washes in tap water and the slides we use. We tried washing only in Distilled water, but the docs said that the "stuff" was still there, probably even a little worse. It's got to be the debris from the etching. I'm planning on doing some trial runs with fresh, clean water in the water bath and with un-etched slides to see if there's a difference. (We empty and wipe out the waterbaths every day, but if we've placed any tissue on an etched slide, I'm sure that powder is floating or mixed in with the water.) The etched slides do not seem powdery or dirty, but they could be microscopically. Any comments or ideas? Thanks in advance, Karen Karen L. Bauer HT(ASCP) Department of Pathology Histology Supervisor Luther Hospital Eau Claire, WI ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. From Charlene.Henry <@t> STJUDE.ORG Tue Jun 17 12:06:16 2008 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Tue Jun 17 12:06:24 2008 Subject: [Histonet] Black artifact on slides. . In-Reply-To: Message-ID: <03E1F5968F60C5448635D49D38B283ED01460FD8AE@SJMEMXMBS11.stjude.sjcrh.local> We have dealt with this problem!!!!!!!! We have found that there is so much glass dust on our slides that we put all of our etched slides in staining trays and wash them before we cut our blocks. This is very time consuming so needless to say I have budgeted to replace this instrument. Charlene -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen Sent: Tuesday, June 17, 2008 11:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Black artifact on slides. . Has anyone encountered black, clumpy "stuff" on slides from using a slide etcher? It could be compared to graphite particles from writing on the slides with pencil, but we do not use pencil anywhere. I'm thinking it could be the dust from the etcher and I'm wondering if anyone else has dealt with this. The pathologists are seeing these particles on our IP slides and Surgical H&E's. Since they are stained by two separate stainers, the only things in common are the washes in tap water and the slides we use. We tried washing only in Distilled water, but the docs said that the "stuff" was still there, probably even a little worse. It's got to be the debris from the etching. I'm planning on doing some trial runs with fresh, clean water in the water bath and with un-etched slides to see if there's a difference. (We empty and wipe out the waterbaths every day, but if we've placed any tissue on an etched slide, I'm sure that powder is floating or mixed in with the water.) The etched slides do not seem powdery or dirty, but they could be microscopically. Any comments or ideas? Thanks in advance, Karen Karen L. Bauer HT(ASCP) Department of Pathology Histology Supervisor Luther Hospital Eau Claire, WI ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgalnow <@t> aol.com Tue Jun 17 12:13:29 2008 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Tue Jun 17 12:13:46 2008 Subject: [Histonet] FW: Anatomic computer systems In-Reply-To: <000001c8d098$77f88040$3d02a8c0@plab.local> References: <000001c8d098$77f88040$3d02a8c0@plab.local> Message-ID: <8CA9EB498D21CB3-140-1E2A@webmail-me05.sysops.aol.com> We have had nothing but bad luck with ours (WindowPath).? If I had my choice, I would go with Co-Path or PowerPath -----Original Message----- From: Cheri Miller To: Histonet@lists.utsouthwestern.edu Cc: histonet-bounces@lists.utsouthwestern.edu Sent: Tue, 17 Jun 2008 12:37 pm Subject: [Histonet] FW: Anatomic computer systems Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 _____ From: Cheri Miller [mailto:cmiller@physlab.com] Sent: Tuesday, June 17, 2008 10:34 AM To: histonet-bounces@lists.utsouthwestern.edu Subject: Anatomic computer systems Time to come out of the dark ages, finally we are getting a computer system for Anatomic path. The powers that be want to have the clinical processing department process our specimens. I need some support here in trying to convince them that clinical and anatomic path are like comparing apples to bananas. My experience with this has always been that the gross room/PA's would data enter and process histology specimens. What do you do?? Who enters, processes and sets up your surgicals? Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Tue Jun 17 12:22:57 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Tue Jun 17 12:23:04 2008 Subject: [Histonet] FW: Anatomic computer systems In-Reply-To: <8CA9EB498D21CB3-140-1E2A@webmail-me05.sysops.aol.com> References: <000001c8d098$77f88040$3d02a8c0@plab.local> <8CA9EB498D21CB3-140-1E2A@webmail-me05.sysops.aol.com> Message-ID: <4857F2F1.1070704@pathology.washington.edu> We are hardcore PowerPath users but there is a new company called PathView that is very promising. We toured their initial site here in Seattle and it was very impressive. I have stayed in touch with the sales rep and they have a new contract in New Orleans. If anyone wants contact info let me know. I have no vested interest in PathView other than to see a good program go under utilized. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. godsgalnow@aol.com wrote: > We have had nothing but bad luck with ours (WindowPath).? If I had my choice, I would go with Co-Path or PowerPath > > > -----Original Message----- > From: Cheri Miller > To: Histonet@lists.utsouthwestern.edu > Cc: histonet-bounces@lists.utsouthwestern.edu > Sent: Tue, 17 Jun 2008 12:37 pm > Subject: [Histonet] FW: Anatomic computer systems > > > > > > > > Cheryl Miller HT (ASCP) > > Histology Supervisor > > Physicians Laboratory,P.C. > > Omaha, Ne. > > 402 738 5052 > > _____ > > From: Cheri Miller [mailto:cmiller@physlab.com] > Sent: Tuesday, June 17, 2008 10:34 AM > To: histonet-bounces@lists.utsouthwestern.edu > Subject: Anatomic computer systems > > > > Time to come out of the dark ages, finally we are getting a computer system > for Anatomic path. The powers that be want to have the clinical processing > department process our specimens. I need some support here in trying to > convince them that clinical and anatomic path are like comparing apples to > bananas. My experience with this has always been that the gross room/PA's > would data enter and process histology specimens. What do you do?? Who > enters, processes and sets up your surgicals? > > > > Cheryl Miller HT (ASCP) > > Histology Supervisor > > Physicians Laboratory,P.C. > > Omaha, Ne. > > 402 738 5052 > > > > > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you > are not the addressee intended / indicated or agent responsible for delivering > it to the addressee, you are hereby notified that you are in possession of > confidential and privileged information. Any dissemination, distribution, or > copying of this e-mail is strictly prohibited. If you have received this > message in error, please notify the sender immediately and delete this email > from your system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JWeems <@t> sjha.org Tue Jun 17 12:24:50 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Jun 17 12:24:51 2008 Subject: [Histonet] FW: Anatomic computer systems In-Reply-To: <000001c8d098$77f88040$3d02a8c0@plab.local> References: <000001c8d098$77f88040$3d02a8c0@plab.local> Message-ID: <982A0A9461F9BF438C7B19A6E425A383245D8D@ITSSSXM01V6.one.ads.che.org> We have dedicated pathology lab assistants or histotechs to accession. We have GE Healthcare's Ultra (formerly Triple G) computer system, but I would recommend CoPath. Good luck! Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax _____ From: Cheri Miller [mailto:cmiller@physlab.com] Sent: Tuesday, June 17, 2008 10:34 AM To: histonet-bounces@lists.utsouthwestern.edu Subject: Anatomic computer systems Time to come out of the dark ages, finally we are getting a computer system for Anatomic path. The powers that be want to have the clinical processing department process our specimens. I need some support here in trying to convince them that clinical and anatomic path are like comparing apples to bananas. My experience with this has always been that the gross room/PA's would data enter and process histology specimens. What do you do?? Who enters, processes and sets up your surgicals? Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From mwich <@t> 7thwavelabs.com Tue Jun 17 12:29:42 2008 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Tue Jun 17 12:29:51 2008 Subject: [Histonet] PAS on GMA Message-ID: <62A8156F8071C8439080D626DF8C33A602E41B@wave-mail.7thwave.local> I'm looking for a protocol for PAS-H that works on GMA-embedded tissue. I tried increasing the times and still the staining is extremely light. Can anyone give me advice on this? Thanks in advance. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From dusko.trajkovic <@t> pfizer.com Tue Jun 17 12:52:36 2008 From: dusko.trajkovic <@t> pfizer.com (Trajkovic, Dusko) Date: Tue Jun 17 12:52:57 2008 Subject: [Histonet] Black artifact on slides. . In-Reply-To: <03E1F5968F60C5448635D49D38B283ED01460FD8AE@SJMEMXMBS11.stjude.sjcrh.local> Message-ID: <3AD0BD3142459B4E9B12CBEAFF2B89B20747C58A@lajamrexm01.amer.pfizer.com> We use Compressed air Duster canisters to blow away the dust on the slides, created by the labeler. It will not entirely eliminate the particles, but it will be much better than what you have now. Dusko -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Henry, Charlene Sent: Tuesday, June 17, 2008 10:06 AM To: 'Bauer, Karen'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Black artifact on slides. . We have dealt with this problem!!!!!!!! We have found that there is so much glass dust on our slides that we put all of our etched slides in staining trays and wash them before we cut our blocks. This is very time consuming so needless to say I have budgeted to replace this instrument. Charlene -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen Sent: Tuesday, June 17, 2008 11:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Black artifact on slides. . Has anyone encountered black, clumpy "stuff" on slides from using a slide etcher? It could be compared to graphite particles from writing on the slides with pencil, but we do not use pencil anywhere. I'm thinking it could be the dust from the etcher and I'm wondering if anyone else has dealt with this. The pathologists are seeing these particles on our IP slides and Surgical H&E's. Since they are stained by two separate stainers, the only things in common are the washes in tap water and the slides we use. We tried washing only in Distilled water, but the docs said that the "stuff" was still there, probably even a little worse. It's got to be the debris from the etching. I'm planning on doing some trial runs with fresh, clean water in the water bath and with un-etched slides to see if there's a difference. (We empty and wipe out the waterbaths every day, but if we've placed any tissue on an etched slide, I'm sure that powder is floating or mixed in with the water.) The etched slides do not seem powdery or dirty, but they could be microscopically. Any comments or ideas? Thanks in advance, Karen Karen L. Bauer HT(ASCP) Department of Pathology Histology Supervisor Luther Hospital Eau Claire, WI ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From khart <@t> gsopath.com Tue Jun 17 12:56:26 2008 From: khart <@t> gsopath.com (Kathy Hart) Date: Tue Jun 17 12:56:38 2008 Subject: [Histonet] Mel-5 Message-ID: <03B63DE44B5C014D84F9A9D8A968B5177407C4@lithium.corp.gsopath.com> Is anyone using Mel 5 (clone Ta99) from any vendor other than Covance/Signet? We are having problems with theirs and are hoping to find another source for that antibody. Kathy S. Hart Lab Manager Greensboro Pathology, LLC 706 Green Valley Rd. Suite 104 Greensboro, NC 27408 phone: 336-387-2513 fax: 336-387-2563 CONFIDENTIALITY NOTICE This message and any included attachments are from Greensboro Pathology, LLC and are intended only for the addressee. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Greensboro Pathology in Greensboro, NC, U.S.A at (+1) (800)345-3376 From Robinsoc <@t> mercyhealth.com Tue Jun 17 13:01:47 2008 From: Robinsoc <@t> mercyhealth.com (Cynthia Robinson) Date: Tue Jun 17 13:02:03 2008 Subject: [Histonet] fixation documention for her2-neu In-Reply-To: <4856B17002000077000037F1@gwmail6.harthosp.org> References: <4856856B.59BC.00AF.0@mercyhealth.com> <4856B17002000077000037F1@gwmail6.harthosp.org> Message-ID: <4857B5BB.59BC.00AF.0@mercyhealth.com> Thanks for all your replies. They have been extremely helpful. Cindi >>> "Richard Cartun" 6/16/2008 5:31 PM >>> I've asked our breast surgeons and interventional radiologists to put the time that the specimen goes into the formalin container (in their office or in radiology) on the pathology requisition. Pathology personnel (residents, fellows, PAs, and attendings) are responsible for noting the time of "formalin contact" on the pathology requisition for excision specimens received from our OR. We are in the process of instituting a 3:30 pm cut-off time for all "large" specimens. Our tissue processors start at 5:30 pm and have 4 hours of formalin on them. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Cynthia Robinson" 06/16/08 4:23 PM >>> We are having quite a time deciding how we are going to handle documentation concerning fixation for specimens requiring Her2-neu IHC testing and interpretation. What is the actual fixation start time? Is it when the large specimen first has 10% formalin added or when the specimen is actually grossed and sections are taken? The CAP guidelines were discussed in Denver at the NSH meeting and I understood that the start time was when the specimen was cut open or 'breadloafed' or when it was grossed in. However, this is not stated very clearly by CAP. What are you doing out there in Histoland? Are you using a general statement on your reports to reflect processing within 6-48 hrs fixation, < 6-48 hrs fixation or > 48 hr fixation or are you actually documenting times when formalin was added, gross time, processor start and end times? Please comment as I need to give my supervisor and the pathologists other perspectives on this controversial issue. Thanks. Cindi Robinson HT(ASCP) Mercy-Sioux City 801 Fifth St Sioux City IA 51101 Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lmdee1 <@t> yahoo.com Tue Jun 17 13:05:45 2008 From: lmdee1 <@t> yahoo.com (Linda) Date: Tue Jun 17 13:05:53 2008 Subject: [Histonet] =?utf-8?q?Tissue_Control_survey=E2=80=8F?= Message-ID: <438333.74554.qm@web36504.mail.mud.yahoo.com> Hello, ? I am interested in what controls you need for your labs-- ? And what you would like to see in your controls(yes, other than them working) ? Such as tissue orientation, a?certain part of the slide?(top by label, middle, bottom) ? Is there certain slide preference?? Poly-L-Lysine, positive charged, Silane, other ? Do you have a label color preference(example-bar coding)? ? What quantity of slides would you like to receive? ? What packaging would you like to receive your slides?in? recyclable, plastic boxes, cardboard boxes, other material suggestions ? Would you like control tissue fixed in another fixative other than 10% neutral buffered formalin? ? Are you limited to vendor contracts or can you choose the vendor that you would like to order controls from? ? What is your expected turn around time-from purchasing placing the order to controls arriving on the shipping dock? ? What price would you consider paying?? per slide/25 slides/50 slides, etc ? Other considerations? ? Thank you for your time and opinions, ? the Histo team From JMahoney <@t> alegent.org Tue Jun 17 13:17:42 2008 From: JMahoney <@t> alegent.org (Mahoney,Janice A) Date: Tue Jun 17 13:21:46 2008 Subject: [Histonet] FW: Anatomic computer systems In-Reply-To: <000001c8d098$77f88040$3d02a8c0@plab.local> References: <000001c8d098$77f88040$3d02a8c0@plab.local> Message-ID: <346E5878979BA54FB4B0BFD6AD93B9B9B037611B53@EXCHMBC1.ad.ah.local> Cheri, I love Cerner Millennium. It is so easy to use and can be built to your specific specifications for many things. Come over to see it you like. Just tell your Cerner rep you want to visit with me about it. Most of the AP labs in Omaha have it. Janice Mahoney HT(ASCP) Histology/Cytology Coordinator Alegent Health Laboratory 4955 F Street Omaha, NE (402)717-2889 fax(402)717-5231 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller [cmiller@physlab.com] Sent: Tuesday, June 17, 2008 11:37 AM To: Histonet@lists.utsouthwestern.edu Cc: histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] FW: Anatomic computer systems Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 _____ From: Cheri Miller [mailto:cmiller@physlab.com] Sent: Tuesday, June 17, 2008 10:34 AM To: histonet-bounces@lists.utsouthwestern.edu Subject: Anatomic computer systems Time to come out of the dark ages, finally we are getting a computer system for Anatomic path. The powers that be want to have the clinical processing department process our specimens. I need some support here in trying to convince them that clinical and anatomic path are like comparing apples to bananas. My experience with this has always been that the gross room/PA's would data enter and process histology specimens. What do you do?? Who enters, processes and sets up your surgicals? Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From strombley <@t> THOCC.ORG Tue Jun 17 13:24:46 2008 From: strombley <@t> THOCC.ORG (Trombley, Sean) Date: Tue Jun 17 13:25:01 2008 Subject: [Histonet] Formalin Recycling Message-ID: <62390770-EC66-4B7B-9E46-D41B100E2F3B@mimectl> Hi All, I was wondering if anyone can give us info on Formalin recycling. Our Safety officer was wondering how cost effective it is as well as user friendly. We are currently having it hauled off site. Thanks Sean Trombley The Hospital of Central CT From mcauliff <@t> umdnj.edu Tue Jun 17 14:07:29 2008 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Jun 17 14:07:15 2008 Subject: [Histonet] freezing artifact In-Reply-To: <3DBACD0C44A1CF429431C0C6DC3E81690316D892@crc_exchange.rcsi-internal.ie> References: <3DBACD0C44A1CF429431C0C6DC3E81690316D892@crc_exchange.rcsi-internal.ie> Message-ID: <48580B71.4000004@umdnj.edu> You are freezing too slowly. Cryoprotection is not a cure-all. Putting the tissue in a -80C freezer is bad practice. Freeze very rapidly with dry ice or 2-methylbutane cooled with liquid nitrogen, THEN put is a pre-cooled vial (or whatever) and put in the freezer. Also, immersion of a whole mouse brain will not provide good fixation of deeper structures. You are almost certainly getting poor morphology that looks like freezing artifact. I suggest fixing by perfusion or slicing the brain into 5 mm sections for fixation. Geoff Aine Behan wrote: > I was looking for advice on troubleshooting already cryoprotected tissue for > freezing artefact (FA). > > > > I have mice brains dissected out after cervical dislocation, postfixed in 4% > Paraformaldehyde/PBS overnight (4 degrees), cryoprotected in 30% sucrose for > ~24 hours (4 degrees) and then placed in -80 freezer. This FA never was an > issue before and the animals are treatment free so anyone know why I would > be getting this now? > > > > Regards, > > > > ?ine > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From sharon.osborn <@t> comcast.net Tue Jun 17 14:31:35 2008 From: sharon.osborn <@t> comcast.net (sharon.osborn@comcast.net) Date: Tue Jun 17 14:31:41 2008 Subject: [Histonet] RE: "dirty" etched slides Message-ID: <061720081931.8186.485811170001A63700001FFA2215561264029D010D9C01D202019D0E089C@comcast.net> TO: Karen Bauer of Mayo, We experienced this problem when I worked at another research facility. Yes, there is glass and paint dust getting on the slides, especially if you are using the "Plus" slides. The electrostatic charge of the slides attracts the dust. Be certain your dust catcher is clean and working properly. This will decrease it. We used the slide etcher for several hours daily because we were cutting several hundred slides daily mostly for IHC development and testing plus H&E and histochemistry. The way we combated this problem was trial and error until we discovered that wash ing the etched slides in hot running water for 15-20 minutes then rinse in 3 changes of absolute alcohol and place in 60 degree oven to dry overnight. decreased the dust particles. You could still see some dust debris in the first two alcohol rinses. The alcohol rinses are to get the water off so the slides are not streaked. Drying the slides before placing the tissues on them gives the clarity they had before we started with the etching. It did not completely remove all the debris but did reduce it enough that there was only occasional interference with the staining or signal. Finally, Leica came out with their slide label system and we demoed and purchased it. The savings in time (fast labeling compared to the etcher), water and quality of slide labeling far outweighed continuing with the etching system. It took several researchers noticing what we were having to do to get good quality labeled slides without the "dust" particles that interfered with the staining to rally for us to get the Leica system. I suggest you consider the Leica slide and cassette labeling system to have dust particle free slides. And this is from a person who first used the TBS slide etching system in 1994 when it was the best we had and we were delighted to have it. However, due to speed and need for greater numbers of slides, there are better systems now available. sharon osborn From jcokie <@t> yahoo.com Tue Jun 17 14:49:07 2008 From: jcokie <@t> yahoo.com (Jeanenne Ely) Date: Tue Jun 17 14:49:11 2008 Subject: [Histonet] Job opening in Houston Message-ID: <228121.48322.qm@web82604.mail.mud.yahoo.com> Hello all- The Veterinary Medicine & Surgery Department at MD Anderson Cancer Center in Houston has two histotech openings available.? One is a Research Histology Technician, and the other is a Senior Research Histology Technician.? Interested parties may access the MD Anderson website at www.mdanderson.org?to read the complete job descriptions, and apply online.? Thanks, Jeanenne Ely, HT (ASCP), QIHC Chief Histology Lab Veterinary Medicine & Surgery, Unit 63 MD Anderson Cancer Center Houston, TX? 77050 (713) 792-2793 From eplurbus <@t> u.washington.edu Tue Jun 17 19:10:39 2008 From: eplurbus <@t> u.washington.edu (eplurbus@u.washington.edu) Date: Tue Jun 17 19:10:43 2008 Subject: [Histonet] Trouble with AcP staining MMA sections Message-ID: Hello, I am having trouble staining some old (7 years) undecalcified, ethanol fixed, methylmethacrylate embedded bone for the presence of acid phosphatase. The blocks that I am trying my protocol on were successfully sectioned and stained by a former technician about four years ago but I haven't been able to duplicate her results. I am using a paraffin embedded cat tooth as control and the osteoclasts stain red every time, but I get no response from the MMA embedded sections. I've tried it after deplasticizing, not deplasticizing (trouble keeping the sections on the slides), re-hydration, without re-hydration.... I feel that my protocol is good and should allow for the detection of AcP but perhaps somewhere along the line I am not handling the MMA embedded tissue appropriately. Is there anyone out there with experience on staining methylmethacrylate embedded tissue for the presence of acid phosphatase that may have a suggestion? I would be happy to post a detailed account of my procedure for someone with experience in these matters. From judith_pardue <@t> memorial.org Wed Jun 18 05:02:56 2008 From: judith_pardue <@t> memorial.org (Pardue, Judith) Date: Wed Jun 18 05:03:18 2008 Subject: [Histonet] Slide labeling Message-ID: <0C063734FB38BC4182A48EAAD838ADBF422307@chimsx04.CHI.catholichealth.net> Our Path lab is changing over to Co-Path, and we were told our slides had to have two patient identifiers. We only put the surgical number on our slides and have not seen any slides from other labs with more than one identifier. Is this is a CAP requirement. Gale Pardue Memorial Health Care System Judith_Pardue@memorial.org This message and accompanying documents are covered by the Electronic Communications Privacy Act 18 U.S.C. "Sections 2510-2521," and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From jqb7 <@t> cdc.gov Wed Jun 18 05:32:20 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Jun 18 05:32:58 2008 Subject: [Histonet] Slide labeling In-Reply-To: <0C063734FB38BC4182A48EAAD838ADBF422307@chimsx04.CHI.catholichealth.net> References: <0C063734FB38BC4182A48EAAD838ADBF422307@chimsx04.CHI.catholichealth.net> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A7F23B55@LTA3VS011.ees.hhs.gov> I found this at CAP....... ANP.21100 Phase II N/A YES NO Are blocks identified adequately? NOTE: Each block of tissue must be identified by the entire accession number assigned to the case and by any descriptive letter(s)/number(s) added by the prosector during the dissection. If additional blocks are prepared later, all lists and logs must reflect these additions. Identification number and letter(s)/numbers(s) must be affixed to all blocks in a manner that remains legible. COMMENTARY: N/A **REVISED** 12/12/2006 ANP.21150 Phase II N/A YES NO Are slides identified permanently with adequate, legible information? NOTE: Each slide must be identified by the entire accession number and descriptive letters unique to the block from which it is cut. Other appropriate identifiers should be included as applicable (e.g., levels of sectioning). Automated prelabeling systems are acceptable. Regardless of whether the identifying information is on the slide or on a label, the information must be indelible, legible and able to withstand all stages of processing and conditions of storage. The laboratory director is responsible for ensuring that slides are adequately, permanently identified. COMMENTARY: N/A REFERENCE: O'Briain DS, et al. Sorting out mix-ups. The provenance of tissue sections may be confirmed by PCR using microsatellite markers. Am J Clin Pathol. 1996;106:758-764. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith Sent: Wednesday, June 18, 2008 6:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide labeling Our Path lab is changing over to Co-Path, and we were told our slides had to have two patient identifiers. We only put the surgical number on our slides and have not seen any slides from other labs with more than one identifier. Is this is a CAP requirement. Gale Pardue Memorial Health Care System Judith_Pardue@memorial.org This message and accompanying documents are covered by the Electronic Communications Privacy Act 18 U.S.C. "Sections 2510-2521," and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Wed Jun 18 05:44:32 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Jun 18 05:44:31 2008 Subject: [Histonet] Slide labeling References: <0C063734FB38BC4182A48EAAD838ADBF422307@chimsx04.CHI.catholichealth.net> <1CE1847DFEA0A647B1CCDE4108EA60A7F23B55@LTA3VS011.ees.hhs.gov> Message-ID: <004401c8d130$4b69f610$0302a8c0@yourxhtr8hvc4p> I believe this two identifier mess came about on the clinical side where CAP and JCAHO instituted this requirement for blood specimens. With HIPAA regulations, one has to wonder if the right hand knows what the left hand is doing. Just my thoughts. JTT ----- Original Message ----- From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" To: "Pardue, Judith" ; Sent: Wednesday, June 18, 2008 5:32 AM Subject: RE: [Histonet] Slide labeling I found this at CAP....... ANP.21100 Phase II N/A YES NO Are blocks identified adequately? NOTE: Each block of tissue must be identified by the entire accession number assigned to the case and by any descriptive letter(s)/number(s) added by the prosector during the dissection. If additional blocks are prepared later, all lists and logs must reflect these additions. Identification number and letter(s)/numbers(s) must be affixed to all blocks in a manner that remains legible. COMMENTARY: N/A **REVISED** 12/12/2006 ANP.21150 Phase II N/A YES NO Are slides identified permanently with adequate, legible information? NOTE: Each slide must be identified by the entire accession number and descriptive letters unique to the block from which it is cut. Other appropriate identifiers should be included as applicable (e.g., levels of sectioning). Automated prelabeling systems are acceptable. Regardless of whether the identifying information is on the slide or on a label, the information must be indelible, legible and able to withstand all stages of processing and conditions of storage. The laboratory director is responsible for ensuring that slides are adequately, permanently identified. COMMENTARY: N/A REFERENCE: O'Briain DS, et al. Sorting out mix-ups. The provenance of tissue sections may be confirmed by PCR using microsatellite markers. Am J Clin Pathol. 1996;106:758-764. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith Sent: Wednesday, June 18, 2008 6:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide labeling Our Path lab is changing over to Co-Path, and we were told our slides had to have two patient identifiers. We only put the surgical number on our slides and have not seen any slides from other labs with more than one identifier. Is this is a CAP requirement. Gale Pardue Memorial Health Care System Judith_Pardue@memorial.org This message and accompanying documents are covered by the Electronic Communications Privacy Act 18 U.S.C. "Sections 2510-2521," and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jessica.Vacca <@t> HCAhealthcare.com Wed Jun 18 09:27:24 2008 From: Jessica.Vacca <@t> HCAhealthcare.com (Vacca Jessica) Date: Wed Jun 18 09:27:34 2008 Subject: [Histonet] Slide labeling In-Reply-To: <004401c8d130$4b69f610$0302a8c0@yourxhtr8hvc4p> References: <0C063734FB38BC4182A48EAAD838ADBF422307@chimsx04.CHI.catholichealth.net><1CE1847DFEA0A647B1CCDE4108EA60A7F23B55@LTA3VS011.ees.hhs.gov> <004401c8d130$4b69f610$0302a8c0@yourxhtr8hvc4p> Message-ID: <41E16A15CE78374EA45B57E0F94339B803F0785C@ORLEV01.hca.corpad.net> I just listened to the latest CAP teleconference and they recommend not even labeling the slides. Their idea was that it would save in additional errors in the event that the wrong pt. Label was placed on the slide. ANP.2150 which is specific to pathology does not state 2 identifiers. So Pathology should just follow that. My opinion only. Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr. Brandon Fl 33511 (813) 571-5193 or (813) 681-5551 ext 2454 Jessica.Vacca@hcahealthcare.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, June 18, 2008 6:45 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Pardue, Judith; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Slide labeling I believe this two identifier mess came about on the clinical side where CAP and JCAHO instituted this requirement for blood specimens. With HIPAA regulations, one has to wonder if the right hand knows what the left hand is doing. Just my thoughts. JTT ----- Original Message ----- From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" To: "Pardue, Judith" ; Sent: Wednesday, June 18, 2008 5:32 AM Subject: RE: [Histonet] Slide labeling I found this at CAP....... ANP.21100 Phase II N/A YES NO Are blocks identified adequately? NOTE: Each block of tissue must be identified by the entire accession number assigned to the case and by any descriptive letter(s)/number(s) added by the prosector during the dissection. If additional blocks are prepared later, all lists and logs must reflect these additions. Identification number and letter(s)/numbers(s) must be affixed to all blocks in a manner that remains legible. COMMENTARY: N/A **REVISED** 12/12/2006 ANP.21150 Phase II N/A YES NO Are slides identified permanently with adequate, legible information? NOTE: Each slide must be identified by the entire accession number and descriptive letters unique to the block from which it is cut. Other appropriate identifiers should be included as applicable (e.g., levels of sectioning). Automated prelabeling systems are acceptable. Regardless of whether the identifying information is on the slide or on a label, the information must be indelible, legible and able to withstand all stages of processing and conditions of storage. The laboratory director is responsible for ensuring that slides are adequately, permanently identified. COMMENTARY: N/A REFERENCE: O'Briain DS, et al. Sorting out mix-ups. The provenance of tissue sections may be confirmed by PCR using microsatellite markers. Am J Clin Pathol. 1996;106:758-764. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith Sent: Wednesday, June 18, 2008 6:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide labeling Our Path lab is changing over to Co-Path, and we were told our slides had to have two patient identifiers. We only put the surgical number on our slides and have not seen any slides from other labs with more than one identifier. Is this is a CAP requirement. Gale Pardue Memorial Health Care System Judith_Pardue@memorial.org This message and accompanying documents are covered by the Electronic Communications Privacy Act 18 U.S.C. "Sections 2510-2521," and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Wed Jun 18 09:59:12 2008 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Wed Jun 18 09:59:26 2008 Subject: [Histonet] Slide labeling Message-ID: <5C0BED61F529364E86309CADEA63FEF20163F432@TRFT-EX01.xRothGen.nhs.uk> Am I dreaming? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vacca Jessica Sent: 18 June 2008 15:27 To: Joe Nocito; Bartlett, Jeanine (CDC/CCID/NCZVED); Pardue, Judith; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide labeling I just listened to the latest CAP teleconference and they recommend not even labeling the slides. Their idea was that it would save in additional errors in the event that the wrong pt. Label was placed on the slide. ANP.2150 which is specific to pathology does not state 2 identifiers. So Pathology should just follow that. My opinion only. Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr. Brandon Fl 33511 (813) 571-5193 or (813) 681-5551 ext 2454 Jessica.Vacca@hcahealthcare.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, June 18, 2008 6:45 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Pardue, Judith; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Slide labeling I believe this two identifier mess came about on the clinical side where CAP and JCAHO instituted this requirement for blood specimens. With HIPAA regulations, one has to wonder if the right hand knows what the left hand is doing. Just my thoughts. JTT ----- Original Message ----- From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" To: "Pardue, Judith" ; Sent: Wednesday, June 18, 2008 5:32 AM Subject: RE: [Histonet] Slide labeling I found this at CAP....... ANP.21100 Phase II N/A YES NO Are blocks identified adequately? NOTE: Each block of tissue must be identified by the entire accession number assigned to the case and by any descriptive letter(s)/number(s) added by the prosector during the dissection. If additional blocks are prepared later, all lists and logs must reflect these additions. Identification number and letter(s)/numbers(s) must be affixed to all blocks in a manner that remains legible. COMMENTARY: N/A **REVISED** 12/12/2006 ANP.21150 Phase II N/A YES NO Are slides identified permanently with adequate, legible information? NOTE: Each slide must be identified by the entire accession number and descriptive letters unique to the block from which it is cut. Other appropriate identifiers should be included as applicable (e.g., levels of sectioning). Automated prelabeling systems are acceptable. Regardless of whether the identifying information is on the slide or on a label, the information must be indelible, legible and able to withstand all stages of processing and conditions of storage. The laboratory director is responsible for ensuring that slides are adequately, permanently identified. COMMENTARY: N/A REFERENCE: O'Briain DS, et al. Sorting out mix-ups. The provenance of tissue sections may be confirmed by PCR using microsatellite markers. Am J Clin Pathol. 1996;106:758-764. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith Sent: Wednesday, June 18, 2008 6:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide labeling Our Path lab is changing over to Co-Path, and we were told our slides had to have two patient identifiers. We only put the surgical number on our slides and have not seen any slides from other labs with more than one identifier. Is this is a CAP requirement. Gale Pardue Memorial Health Care System Judith_Pardue@memorial.org This message and accompanying documents are covered by the Electronic Communications Privacy Act 18 U.S.C. "Sections 2510-2521," and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> pathview.com Wed Jun 18 10:31:12 2008 From: mike <@t> pathview.com (Michael Mihalik) Date: Wed Jun 18 10:31:45 2008 Subject: [Histonet] Slide labeling In-Reply-To: <41E16A15CE78374EA45B57E0F94339B803F0785C@ORLEV01.hca.corpad.net> References: <0C063734FB38BC4182A48EAAD838ADBF422307@chimsx04.CHI.catholichealth.net><1CE1847DFEA0A647B1CCDE4108EA60A7F23B55@LTA3VS011.ees.hhs.gov><004401c8d130$4b69f610$0302a8c0@yourxhtr8hvc4p> <41E16A15CE78374EA45B57E0F94339B803F0785C@ORLEV01.hca.corpad.net> Message-ID: <1029EB3512F14FA190EB99EF028C2BA8@MDMM1330> On top of this is the entire HIPPA thing about deidentifying slides for sendout purposes. In fact my latest client simply has a case # and barcode -- no patient name whatsoever. Michael Mihalik PathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vacca Jessica Sent: Wednesday, June 18, 2008 7:27 AM To: Joe Nocito; Bartlett, Jeanine (CDC/CCID/NCZVED); Pardue, Judith; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide labeling I just listened to the latest CAP teleconference and they recommend not even labeling the slides. Their idea was that it would save in additional errors in the event that the wrong pt. Label was placed on the slide. ANP.2150 which is specific to pathology does not state 2 identifiers. So Pathology should just follow that. My opinion only. Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr. Brandon Fl 33511 (813) 571-5193 or (813) 681-5551 ext 2454 Jessica.Vacca@hcahealthcare.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, June 18, 2008 6:45 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Pardue, Judith; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Slide labeling I believe this two identifier mess came about on the clinical side where CAP and JCAHO instituted this requirement for blood specimens. With HIPAA regulations, one has to wonder if the right hand knows what the left hand is doing. Just my thoughts. JTT ----- Original Message ----- From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" To: "Pardue, Judith" ; Sent: Wednesday, June 18, 2008 5:32 AM Subject: RE: [Histonet] Slide labeling I found this at CAP....... ANP.21100 Phase II N/A YES NO Are blocks identified adequately? NOTE: Each block of tissue must be identified by the entire accession number assigned to the case and by any descriptive letter(s)/number(s) added by the prosector during the dissection. If additional blocks are prepared later, all lists and logs must reflect these additions. Identification number and letter(s)/numbers(s) must be affixed to all blocks in a manner that remains legible. COMMENTARY: N/A **REVISED** 12/12/2006 ANP.21150 Phase II N/A YES NO Are slides identified permanently with adequate, legible information? NOTE: Each slide must be identified by the entire accession number and descriptive letters unique to the block from which it is cut. Other appropriate identifiers should be included as applicable (e.g., levels of sectioning). Automated prelabeling systems are acceptable. Regardless of whether the identifying information is on the slide or on a label, the information must be indelible, legible and able to withstand all stages of processing and conditions of storage. The laboratory director is responsible for ensuring that slides are adequately, permanently identified. COMMENTARY: N/A REFERENCE: O'Briain DS, et al. Sorting out mix-ups. The provenance of tissue sections may be confirmed by PCR using microsatellite markers. Am J Clin Pathol. 1996;106:758-764. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith Sent: Wednesday, June 18, 2008 6:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide labeling Our Path lab is changing over to Co-Path, and we were told our slides had to have two patient identifiers. We only put the surgical number on our slides and have not seen any slides from other labs with more than one identifier. Is this is a CAP requirement. Gale Pardue Memorial Health Care System Judith_Pardue@memorial.org This message and accompanying documents are covered by the Electronic Communications Privacy Act 18 U.S.C. "Sections 2510-2521," and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CarterK <@t> MedImmune.com Wed Jun 18 10:39:08 2008 From: CarterK <@t> MedImmune.com (Carter, Kendra) Date: Wed Jun 18 10:39:19 2008 Subject: [Histonet] Archiving Software Message-ID: <4FF56D2ABFD3FE47A0C41D5631372133ACF2DE@MD1EV001.medimmune.com> Hello Everyone, Does anyone have any reccomendations for a software program that is user friendly to use for tracking slides and paraffin blocks? It would be helpful if the software program could be GLP if necessary. THANKS!! Kendra Leigh Carter GLP Documentation & Archive Specialist MedImmune One MedImmune Way Gaithersburg, MD 20878 PH: 301-398-4956 Fax: 301-398-9956 To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From Bonnie.Whitaker <@t> osumc.edu Wed Jun 18 12:10:42 2008 From: Bonnie.Whitaker <@t> osumc.edu (Whitaker, Bonnie) Date: Wed Jun 18 12:10:54 2008 Subject: [Histonet] Ventana Discovery Message-ID: <3CE20ED86C4A114EBDF3BCE8DEFD8F602624EC@msxc06.OSUMC.EDU> Hi All, Is anyone out there using the Ventana Discovery, and who wouldn't mind a phone call from someone here at OSU with some questions about it? Please let me know! Thanks, Bonnie Whitaker Clinical Histology Manager Ohio State University Medical Center N308B Doan Hall 410 W. 10th Ave. Columbus, OH 43210 Bonnie.Whitaker@osumc.edu phone 614.293.5048 fax 614.293.7273 pager 614.346.5013 From nefff <@t> staff.uni-marburg.de Wed Jun 18 12:20:40 2008 From: nefff <@t> staff.uni-marburg.de (Dr. med. Frauke Neff) Date: Wed Jun 18 12:20:45 2008 Subject: [Histonet] IF on paraffin material Message-ID: <1213809640.485943e82aab0@webmail.med.uni-marburg.de> Dear Histonetters! I'm trying for several weeks to get the Immunofluorescence on paraffin material started, but it does not work. Maybe my slides are to thick with 3?m? Or maybe its because I use an inverted Microscope for havind a look on the slides? Does the excitation not penetrate the tissue and do the not glow like i expect them to do? Because if I use IF on cells fixed with PFA, it works fine. Does anyone of you have an idea on that problem or can provide me with a protocol that works fine? (I currently use the abcam protocol on their homepage) I thank you all in advance for your help, yours Frauke ---------------------------------------------------------------- This message was sent using IMP, the Internet Messaging Program. From pruegg <@t> ihctech.net Tue Jun 17 12:46:49 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Jun 18 14:45:54 2008 Subject: [Histonet] bone stuff Message-ID: <001301c8d0a2$21731a50$6401a8c0@Patsyoffice> To Bone Heads, It has been a while since I have done IHC for bone samples. Anybody out there doing IHC on ffpe formic acid decaled rat bone for Osteocalcin and Bone Sialoprotein? Is there a non mouse ab for these? The last time I used them I used mouse monoclonals from U of Iowa Hybridoma Bank?? Also, anybody heard of Runx2 or Stro-1 Abs for these? Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From ebreisch <@t> rchsd.org Wed Jun 18 14:55:53 2008 From: ebreisch <@t> rchsd.org (Breisch, Eric) Date: Wed Jun 18 14:56:25 2008 Subject: [Histonet] CD-19 immunohistochemistry Message-ID: <43B97B4C402C2C44AAA2A8D2C86A88B31A5AC9@e2k3backend1.RCHSD.org> We are looking for someone that can do CD-19 IHC. If anyone out there can do that particular antibody could they please contact me and provide me with the correct billing information? Your help is greatly appreciated. Thank you. Eric Eric A. Breisch, Ph.D. Clinical Anatomist Dept. of Pathology Rady Children's Hospital and Health Center Associate Clinical Professor of Anatomy Dept. of Surgery UCSD School of Medicine From histoinfo <@t> comcast.net Wed Jun 18 15:13:50 2008 From: histoinfo <@t> comcast.net (histoinfo@comcast.net) Date: Wed Jun 18 15:13:58 2008 Subject: [Histonet] Animal Tissue processing question Message-ID: <061820082013.5991.48596C7E000A512100001767221655140601000207019B9C0708@comcast.net> Dear Histonetters, After doing human histology for 15 years I am excited to have the opportunity to help start up a brand new research histology laboratory. It is a very small lab so I will still have my day job. But it is all new and different and a bit exciting. I would like to ask your help with the processing times. I know animal tissue is dryer so I am thinking less time in alcohol but am looking to those of you out there with the experience for a place to start when it comes to VIP processing times. I know I will be processing tissues from pigs and primates if that makes a difference. Brand new VIP, brand new embedding center, brand new microtome. . . . I can't tell you how exciting this is. Thanks for all your help. Jennifer From John.Spair <@t> multicare.org Wed Jun 18 15:30:25 2008 From: John.Spair <@t> multicare.org (John Spair) Date: Wed Jun 18 15:30:37 2008 Subject: [Histonet] RE: Histonet Digest, Vol 55, Issue 29 References: <64479E9E47W62457-01@MMS_multicare.org> Message-ID: <61A9977919846C479389493BAE2517CA015309C4@MHSEXMBX1.multicare.org> We've been using CoPath for about 7 years now, and we print out our slide labels from CoPath that has both accession number and patient's name. You can have your slide label designed however you want it practically, so if you only want accession number and no name, tell them that. Making labels changes in CoPath is not hard. We are accredited by both CAP and JCAHO, and CAP doesn't specify how many identifiers should be on a slide label - but I believe JCAHO refers to 2 patient identifiers. As with probably most hospital sites, generally if you're a CAP accredited laboratory, JCAHO doesn't really inspect you - besides perhaps doing a walk through and looking for things like safety issues, envrionmental issues, competency, etc. I've often thought if I had to use 2 identifiers on everything, wouldn't that be a bit much to put on a paraffin block? Good luck with your CoPath implementation. ----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu ] On Behalf Of Pardue, Judith Sent: Wednesday, June 18, 2008 6:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide labeling Our Path lab is changing over to Co-Path, and we were told our slides had to have two patient identifiers. We only put the surgical number on our slides and have not seen any slides from other labs with more than one identifier. Is this is a CAP requirement. Gale Pardue Memorial Health Care System Judith_Pardue@memorial.org John Spair, Manager Pathology Services LABORATORIES Northwest MultiCare Health System PO Box 5299, Tacoma WA 98415 Phone: 253-403-6090 Fax: 253-403-1357 "MMS " made the following annotations. ------------------------------------------------------------------------------ NOTICE: This e-mail and the attachments hereto, if any, may contain privileged and/or confidential information. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, you are hereby notified that any examination, distribution or copying of this e-mail and the attachments hereto, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by email or telephone and permanently delete this e-mail and the attachments hereto, if any, and destroy any printout thereof. MultiCare Health System, Tacoma, WA 98415 (253) 403-1000. ============================================================================== From ChaseM <@t> childrensdayton.org Wed Jun 18 16:01:46 2008 From: ChaseM <@t> childrensdayton.org (Matthew Chase) Date: Wed Jun 18 16:02:37 2008 Subject: [Histonet] Dayton Ohio Position Message-ID: Hey All Well the person that said they would never leave...left. So our histology tech position is open. We have one part time, one full time (that could be you) and myself. We process about 5000 cases a year, we average about 30 blocks a day. Mondays are kinda heavy with around 70 blocks the rest of the week is easy. This is Dayton Children's Hospital, good benefits, not a whole lot of stress. If your looking for a great place with a great group of people give me a call, or call HR at 937-641-8090 and ask for Dan Krauss. Matt Chase, HT (ASCP) Supervisor of Pathology Children's Medical Center Dayton, Ohio (937) 641-3000 ext 8229 chasem@childrensdayton.org From TJJ <@t> Stowers-Institute.org Wed Jun 18 16:24:59 2008 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Wed Jun 18 16:25:24 2008 Subject: [Histonet] RNAses on glass slides Message-ID: Dearest colleagues, Again I need some information that can be exceedingly difficult to find in the literature. There is a question here as to whether slides used for ISH (in our case, plus/charged slides) need to be treated with DEPC or other RNAse inhibitor prior to using to mount tissue sections for subsquent mRNA ISH. We have never done this and the practice is now coming into question as a possible mechanism for giving inconsistent results with a researcher's project. Our current practice includes new blade, new box of slides, use gloves, wipe down the microtome and all utensils with RNAse Away, clean out waterbath glass and use DEPC water in waterbath. Up until now, it seems to have been adquate. Thank for any help you can give me. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From mohs76009 <@t> yahoo.com Wed Jun 18 16:25:57 2008 From: mohs76009 <@t> yahoo.com (Matt Bancroft) Date: Wed Jun 18 16:26:02 2008 Subject: [Histonet] Mohs Histotech Position Message-ID: <101481.7089.qm@web63411.mail.re1.yahoo.com> Fort Worth, TX--Full-time Mohs Histotech position in busy Mohs surgery private practice. You will be working with a team-oriented group consisting of a Mohs surgeon and staff. Applicant should be experienced in the preparation of high quality frozen sections, and be comfortable with a high case volume. Mohs experience preferred, but will consider applicants with histotech background willing to transition into Mohs. Outstanding compensation and benefits package. Please e-mail your resume to dmarra@post.harvard.edu. From mohs76009 <@t> yahoo.com Wed Jun 18 16:27:12 2008 From: mohs76009 <@t> yahoo.com (Matt Bancroft) Date: Wed Jun 18 16:27:16 2008 Subject: [Histonet] Mohs Histotech Position In-Reply-To: <3CE20ED86C4A114EBDF3BCE8DEFD8F602624EC@msxc06.OSUMC.EDU> Message-ID: <712600.59817.qm@web63409.mail.re1.yahoo.com> Fort Worth, TX--Full-time Mohs Histotech position in busy Mohs surgery private practice. You will be working with a team-oriented group consisting of a Mohs surgeon and staff. Applicant should be experienced in the preparation of high quality frozen sections, and be comfortable with a high case volume. Mohs experience preferred, but will consider applicants with histotech background willing to transition into Mohs. Outstanding compensation and benefits package. Please e-mail your resume to dmarra@post.harvard.edu. From liz <@t> premierlab.com Wed Jun 18 18:20:02 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Jun 18 18:20:09 2008 Subject: [Histonet] DHE staining of mouse femoral arteries Message-ID: Hello all Is there anyone out there that can done DHE supraoxide staining of frozen section mouse femoral arteries. We just can't seem to get it to work. Any advice would be appreciated. thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 From jnocito <@t> satx.rr.com Wed Jun 18 18:35:17 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Jun 18 18:34:58 2008 Subject: [Histonet] Slide labeling References: <5C0BED61F529364E86309CADEA63FEF20163F432@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <002801c8d19b$f7a0dd70$0302a8c0@yourxhtr8hvc4p> more like nightmaring ----- Original Message ----- From: "Marshall Terry Dr, Consultant Histopathologist" To: "Vacca Jessica" ; "Joe Nocito" ; "Bartlett, Jeanine (CDC/CCID/NCZVED)" ; "Pardue, Judith" ; Sent: Wednesday, June 18, 2008 9:59 AM Subject: RE: [Histonet] Slide labeling Am I dreaming? Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vacca Jessica Sent: 18 June 2008 15:27 To: Joe Nocito; Bartlett, Jeanine (CDC/CCID/NCZVED); Pardue, Judith; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide labeling I just listened to the latest CAP teleconference and they recommend not even labeling the slides. Their idea was that it would save in additional errors in the event that the wrong pt. Label was placed on the slide. ANP.2150 which is specific to pathology does not state 2 identifiers. So Pathology should just follow that. My opinion only. Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr. Brandon Fl 33511 (813) 571-5193 or (813) 681-5551 ext 2454 Jessica.Vacca@hcahealthcare.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, June 18, 2008 6:45 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Pardue, Judith; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Slide labeling I believe this two identifier mess came about on the clinical side where CAP and JCAHO instituted this requirement for blood specimens. With HIPAA regulations, one has to wonder if the right hand knows what the left hand is doing. Just my thoughts. JTT ----- Original Message ----- From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" To: "Pardue, Judith" ; Sent: Wednesday, June 18, 2008 5:32 AM Subject: RE: [Histonet] Slide labeling I found this at CAP....... ANP.21100 Phase II N/A YES NO Are blocks identified adequately? NOTE: Each block of tissue must be identified by the entire accession number assigned to the case and by any descriptive letter(s)/number(s) added by the prosector during the dissection. If additional blocks are prepared later, all lists and logs must reflect these additions. Identification number and letter(s)/numbers(s) must be affixed to all blocks in a manner that remains legible. COMMENTARY: N/A **REVISED** 12/12/2006 ANP.21150 Phase II N/A YES NO Are slides identified permanently with adequate, legible information? NOTE: Each slide must be identified by the entire accession number and descriptive letters unique to the block from which it is cut. Other appropriate identifiers should be included as applicable (e.g., levels of sectioning). Automated prelabeling systems are acceptable. Regardless of whether the identifying information is on the slide or on a label, the information must be indelible, legible and able to withstand all stages of processing and conditions of storage. The laboratory director is responsible for ensuring that slides are adequately, permanently identified. COMMENTARY: N/A REFERENCE: O'Briain DS, et al. Sorting out mix-ups. The provenance of tissue sections may be confirmed by PCR using microsatellite markers. Am J Clin Pathol. 1996;106:758-764. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith Sent: Wednesday, June 18, 2008 6:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide labeling Our Path lab is changing over to Co-Path, and we were told our slides had to have two patient identifiers. We only put the surgical number on our slides and have not seen any slides from other labs with more than one identifier. Is this is a CAP requirement. Gale Pardue Memorial Health Care System Judith_Pardue@memorial.org This message and accompanying documents are covered by the Electronic Communications Privacy Act 18 U.S.C. "Sections 2510-2521," and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tjasper <@t> copc.net Wed Jun 18 18:36:57 2008 From: tjasper <@t> copc.net (Thomas Jasper) Date: Wed Jun 18 18:37:03 2008 Subject: [Histonet] FW: Anatomic computer systems References: <000001c8d098$77f88040$3d02a8c0@plab.local> Message-ID: <90354A475B420441B2A0396E5008D4965E20E4@copc-sbs.COPC.local> Hey Cheryl, I am in total agreement with you about having AP personnel handling case accessioning for histology. Accessioning/logging in cases may not be rocket science. That said, it is important to be consistent and pay close attention when this task is performed. It is difficult enough having pathology personnel get things correctly entered all the time. Having clinical lab staff accurately and efficiently entering path specimens into your AP LIS is an unreal expectation. This is not the fault of the clinical lab. There is a completely different mind-set at work for the processing of clinical lab specimens. Not knowing the nature and scope of your service, I'd venture to guess that you receive specimens from multiple locations. This creates a level of complexity for billing, which, from an administrative standpoint, is best served by having AP personnel handle the specimens. AP personnel also (for the most part) are aware of the downstream activity associated with path specimens, such as IHC and special stain panels, levels and extra unstained cuts, etc. To flip it around, how much sense would it make for AP staff to log in general lab specimens? Bad idea in my book. Again, not a realistic approach, and ultimately a patient care issue. At my previous employer we switched from PowerPath to SSC (SoftPath). This was unfavorable in my opinion, but that's another story. Anyway, since Soft was "integrated" we needed to have patients entered into the Soft system before AP could be done on them. Having non-AP personnel do this (general demographics, etc.) was ok, but in AP we still brought up the patients and entered the appropriate data for our purposes. My point being...that is the limit I'd have non-AP personnel involved. We actually were forced into this practice as we no longer had a connection to registration in AP. Also, your point about anatomic and clinical being like apples and bananas is right on. I would tell my former lab administrators on a regular basis that AP does not function well by forcing the "yoke" of clinical lab upon it. This was a frustrating point. Many administrators do not understand AP as they come from the clinical lab world. Most believe the methods and practices they're familiar with will apply to AP. I'm sure I don't need to preach to the choir here, but it's just not the same. As far as LIS systems go, I'm happy to say that my current employer uses PowerPath. In my opinion PowerPath is one of the best AP LIS systems available. I do not mean to negatively criticize any other systems that I've no personal experience with. I would however be less than thrilled if I were forced to switch from PowerPath to Soft again. To be fair, Soft works for some people and probably has it's advocates, it's just not my favorite. Good luck, Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, OR 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Tuesday, June 17, 2008 9:38 AM To: Histonet@lists.utsouthwestern.edu Cc: histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] FW: Anatomic computer systems Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 _____ From: Cheri Miller [mailto:cmiller@physlab.com] Sent: Tuesday, June 17, 2008 10:34 AM To: histonet-bounces@lists.utsouthwestern.edu Subject: Anatomic computer systems Time to come out of the dark ages, finally we are getting a computer system for Anatomic path. The powers that be want to have the clinical processing department process our specimens. I need some support here in trying to convince them that clinical and anatomic path are like comparing apples to bananas. My experience with this has always been that the gross room/PA's would data enter and process histology specimens. What do you do?? Who enters, processes and sets up your surgicals? Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lynnw <@t> nf.sympatico.ca Wed Jun 18 19:48:17 2008 From: lynnw <@t> nf.sympatico.ca (Lynn Wade) Date: Wed Jun 18 19:48:19 2008 Subject: [Histonet] Junk Message-ID: <004b01c8d1a6$2a092d80$0a02a8c0@wadeq8fg62kuom> Is it possible for the list owner to request that users not re-send all the previous message(s) with their response? It is really annoying to be trying to read down through the junk to get to the next question or response. Thanks, Lynn Wade St. John's, Newfoundland & Labrador From kmerriam2003 <@t> yahoo.com Thu Jun 19 06:34:18 2008 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Thu Jun 19 06:34:32 2008 Subject: [Histonet] Animal Tissue processing question Message-ID: <882388.3664.qm@web50307.mail.re2.yahoo.com> Hi Jennifer, The NSH publishes an "Animal Processing Manual", I don't think it costs very much ($30 for members) and it lists the processing schedules for many different species; I believe that most of these schedules were submitted by VIR researchers. Here is the link: http://www.nsh.org/organizations.php3?action=printContentItem&orgid=111&typeID=1157&itemID=18328 ?Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: "histoinfo@comcast.net" To: Histonet@lists.utsouthwestern.edu Sent: Wednesday, June 18, 2008 4:13:50 PM Subject: [Histonet] Animal Tissue processing question Dear Histonetters, After doing human histology for 15 years I am excited to have the opportunity to help start up a brand new research histology laboratory. It is a very small lab so I will still have my day job. But it is all new and different and a bit exciting. I would like to ask your help with the processing times. I know animal tissue is dryer so I am thinking less time in alcohol but am looking to those of you out there with the experience for a place to start when it comes to VIP processing times.? I know I will be processing tissues from pigs and primates if that makes a difference. Brand new VIP, brand new embedding center, brand new microtome. . . . I can't tell you how exciting this is. Thanks for all your help. Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From judith_pardue <@t> memorial.org Thu Jun 19 06:47:26 2008 From: judith_pardue <@t> memorial.org (Pardue, Judith) Date: Thu Jun 19 06:47:51 2008 Subject: [Histonet] IMPLANTS Message-ID: <0C063734FB38BC4182A48EAAD838ADBF422532@chimsx04.CHI.catholichealth.net> Does anyone know what the federal guidelines are for breast implants, how long we are required to keep them? Judith Pardue Memorial Health Care System Chattanooga, Tennessee Judith_Pardue@memorial.org This message and accompanying documents are covered by the Electronic Communications Privacy Act 18 U.S.C. "Sections 2510-2521," and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From rjbuesa <@t> yahoo.com Thu Jun 19 07:09:45 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jun 19 07:09:53 2008 Subject: [Histonet] Animal Tissue processing question In-Reply-To: <061820082013.5991.48596C7E000A512100001767221655140601000207019B9C0708@comcast.net> Message-ID: <180059.96232.qm@web65701.mail.ac4.yahoo.com> You just mentioned 2 different species with peculiar characteristics. The difficulty with veterinary histology is that you will have to modify your procedures slightly to each, and you should never process tissues of different species in the same run. From very "dry" mice tissues with less dehydration times, to pig tissues requiring more time in the antemedium because of the tisular fat (unless it is a boar) you will have to prepare modified protocol. My advise, write down your protocols and the animals you used them with and evaluate the results in a manner that in some time you will have the protocols you need. Sorry if this is not what you asked for, but, as I wrote you, there should be specific protocols. Ren? J. histoinfo@comcast.net wrote: Dear Histonetters, After doing human histology for 15 years I am excited to have the opportunity to help start up a brand new research histology laboratory. It is a very small lab so I will still have my day job. But it is all new and different and a bit exciting. I would like to ask your help with the processing times. I know animal tissue is dryer so I am thinking less time in alcohol but am looking to those of you out there with the experience for a place to start when it comes to VIP processing times. I know I will be processing tissues from pigs and primates if that makes a difference. Brand new VIP, brand new embedding center, brand new microtome. . . . I can't tell you how exciting this is. Thanks for all your help. Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Jun 19 07:13:17 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jun 19 07:13:20 2008 Subject: [Histonet] IMPLANTS In-Reply-To: <0C063734FB38BC4182A48EAAD838ADBF422532@chimsx04.CHI.catholichealth.net> Message-ID: <383178.39833.qm@web65703.mail.ac4.yahoo.com> In 2 words: for ever! Don't mind the federal guidelines, listen to your legal department. Ren? J. "Pardue, Judith" wrote: Does anyone know what the federal guidelines are for breast implants, how long we are required to keep them? Judith Pardue Memorial Health Care System Chattanooga, Tennessee Judith_Pardue@memorial.org This message and accompanying documents are covered by the Electronic Communications Privacy Act 18 U.S.C. "Sections 2510-2521," and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Thu Jun 19 08:16:52 2008 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Thu Jun 19 08:16:59 2008 Subject: [Histonet] RNAses on glass slides In-Reply-To: References: Message-ID: <63EA0607835FBA4689CEA9EA8B482692012B1BBF@usctmx1141.merck.com> Teri, You should either treat the slides with DEPC or you can bake them at 250C for 24 hrs to destroy RNases. Brett M. Connolly, Ph.D. Research Fellow, Imaging Research Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Teri Sent: Wednesday, June 18, 2008 5:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RNAses on glass slides Dearest colleagues, Again I need some information that can be exceedingly difficult to find in the literature. There is a question here as to whether slides used for ISH (in our case, plus/charged slides) need to be treated with DEPC or other RNAse inhibitor prior to using to mount tissue sections for subsquent mRNA ISH. We have never done this and the practice is now coming into question as a possible mechanism for giving inconsistent results with a researcher's project. Our current practice includes new blade, new box of slides, use gloves, wipe down the microtome and all utensils with RNAse Away, clean out waterbath glass and use DEPC water in waterbath. Up until now, it seems to have been adquate. Thank for any help you can give me. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From talulahgosh <@t> gmail.com Thu Jun 19 08:28:40 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Jun 19 08:28:45 2008 Subject: [Histonet] RNAses on glass slides In-Reply-To: <63EA0607835FBA4689CEA9EA8B482692012B1BBF@usctmx1141.merck.com> References: <63EA0607835FBA4689CEA9EA8B482692012B1BBF@usctmx1141.merck.com> Message-ID: We don't treat our slides because they come clean already (Fisher Superfrost plus) When our in situ's don't work, we just make all of the solutions over and buy new NBT/BCIP and DIG antibody. This has always worked for us, though it can get expensive! You can check if your probe is working by dotting it on filter paper and going through your protocol (obviously you can skip stuff like proteinase K or fixation). This will also check all of your reagents. Also, make sure the RNase Away is not left on your utensils, we do a short rinse with DEPC water after treating them. One other small thing--check your hybridization oven temperature with a thermometer. Our digital reading has always been off by five degrees. Emily -- When you're riding in a time machine way far into the future, don't stick your elbow out the window, or it'll turn into a fossil. From victor <@t> pathology.washington.edu Thu Jun 19 09:01:08 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Thu Jun 19 09:01:12 2008 Subject: [Histonet] Animal Tissue processing question In-Reply-To: <180059.96232.qm@web65701.mail.ac4.yahoo.com> References: <180059.96232.qm@web65701.mail.ac4.yahoo.com> Message-ID: <485A66A4.7030309@pathology.washington.edu> Having worked previously in a high volume vet diagnostic lab I somewhat disagree with Rene. For optimal processing I agree, but not for practicality. On a daily basis we would have every species under the sun (almost) and they got grouped together on one processor. Some days certain tissues might be a little more difficult, but not impossible. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Rene J Buesa wrote: > You just mentioned 2 different species with peculiar characteristics. The difficulty with veterinary histology is that you will have to modify your procedures slightly to each, and you should never process tissues of different species in the same run. > From very "dry" mice tissues with less dehydration times, to pig tissues requiring more time in the antemedium because of the tisular fat (unless it is a boar) you will have to prepare modified protocol. > My advise, write down your protocols and the animals you used them with and evaluate the results in a manner that in some time you will have the protocols you need. > Sorry if this is not what you asked for, but, as I wrote you, there should be specific protocols. > Ren? J. > > histoinfo@comcast.net wrote: > Dear Histonetters, > After doing human histology for 15 years I am excited to have the opportunity to help start up a brand new research histology laboratory. It is a very small lab so I will still have my day job. But it is all new and different and a bit exciting. I would like to ask your help with the processing times. I know animal tissue is dryer so I am thinking less time in alcohol but am looking to those of you out there with the experience for a place to start when it comes to VIP processing times. I know I will be processing tissues from pigs and primates if that makes a difference. Brand new VIP, brand new embedding center, brand new microtome. . . . I can't tell you how exciting this is. Thanks for all your help. > Jennifer > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rfields <@t> gidocs.net Thu Jun 19 09:30:11 2008 From: rfields <@t> gidocs.net (Rosa Fields) Date: Thu Jun 19 09:30:21 2008 Subject: [Histonet] Animal Tissue processing question In-Reply-To: <485A66A4.7030309@pathology.washington.edu> References: <180059.96232.qm@web65701.mail.ac4.yahoo.com> <485A66A4.7030309@pathology.washington.edu> Message-ID: <2F2611250DCD6549AA3D96CE8AF1F0180112462E@giexchange.gidocs.net> I would have to agree with Victor, also previously from a high volume vet diagnostic lab, as a matter of practicality all of our species were also grouped onto one processor. It is very challenging to practically deal with everything one may see in a vet diagnostic lab! Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Thursday, June 19, 2008 9:01 AM To: Rene J Buesa Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Animal Tissue processing question Having worked previously in a high volume vet diagnostic lab I somewhat disagree with Rene. For optimal processing I agree, but not for practicality. On a daily basis we would have every species under the sun (almost) and they got grouped together on one processor. Some days certain tissues might be a little more difficult, but not impossible. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Rene J Buesa wrote: > You just mentioned 2 different species with peculiar characteristics. The difficulty with veterinary histology is that you will have to modify your procedures slightly to each, and you should never process tissues of different species in the same run. > From very "dry" mice tissues with less dehydration times, to pig tissues requiring more time in the antemedium because of the tisular fat (unless it is a boar) you will have to prepare modified protocol. > My advise, write down your protocols and the animals you used them with and evaluate the results in a manner that in some time you will have the protocols you need. > Sorry if this is not what you asked for, but, as I wrote you, there should be specific protocols. > Ren? J. > > histoinfo@comcast.net wrote: > Dear Histonetters, > After doing human histology for 15 years I am excited to have the opportunity to help start up a brand new research histology laboratory. It is a very small lab so I will still have my day job. But it is all new and different and a bit exciting. I would like to ask your help with the processing times. I know animal tissue is dryer so I am thinking less time in alcohol but am looking to those of you out there with the experience for a place to start when it comes to VIP processing times. I know I will be processing tissues from pigs and primates if that makes a difference. Brand new VIP, brand new embedding center, brand new microtome. . . . I can't tell you how exciting this is. Thanks for all your help. > Jennifer > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ryaskovich <@t> dir.nidcr.nih.gov Thu Jun 19 09:37:11 2008 From: ryaskovich <@t> dir.nidcr.nih.gov (Yaskovich, Ruth A (NIH/NIDCR) [E]) Date: Thu Jun 19 09:37:27 2008 Subject: [Histonet] Animal Tissue processing question In-Reply-To: <485A66A4.7030309@pathology.washington.edu> References: <180059.96232.qm@web65701.mail.ac4.yahoo.com> <485A66A4.7030309@pathology.washington.edu> Message-ID: Victor, I agree I have run every species together and got beautiful results for my lab it was all about the fixation and fixation times. Ruth Yaskovich N.I.H. -----Original Message----- From: Victor Tobias [mailto:victor@pathology.washington.edu] Sent: Thursday, June 19, 2008 10:01 AM To: Rene J Buesa Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Animal Tissue processing question Having worked previously in a high volume vet diagnostic lab I somewhat disagree with Rene. For optimal processing I agree, but not for practicality. On a daily basis we would have every species under the sun (almost) and they got grouped together on one processor. Some days certain tissues might be a little more difficult, but not impossible. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Rene J Buesa wrote: > You just mentioned 2 different species with peculiar characteristics. The difficulty with veterinary histology is that you will have to modify your procedures slightly to each, and you should never process tissues of different species in the same run. > From very "dry" mice tissues with less dehydration times, to pig tissues requiring more time in the antemedium because of the tisular fat (unless it is a boar) you will have to prepare modified protocol. > My advise, write down your protocols and the animals you used them with and evaluate the results in a manner that in some time you will have the protocols you need. > Sorry if this is not what you asked for, but, as I wrote you, there should be specific protocols. > Ren? J. > > histoinfo@comcast.net wrote: > Dear Histonetters, > After doing human histology for 15 years I am excited to have the opportunity to help start up a brand new research histology laboratory. It is a very small lab so I will still have my day job. But it is all new and different and a bit exciting. I would like to ask your help with the processing times. I know animal tissue is dryer so I am thinking less time in alcohol but am looking to those of you out there with the experience for a place to start when it comes to VIP processing times. I know I will be processing tissues from pigs and primates if that makes a difference. Brand new VIP, brand new embedding center, brand new microtome. . . . I can't tell you how exciting this is. Thanks for all your help. > Jennifer > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SAllen <@t> exchange.hsc.mb.ca Thu Jun 19 09:40:06 2008 From: SAllen <@t> exchange.hsc.mb.ca (Sharon Allen) Date: Thu Jun 19 09:40:41 2008 Subject: [Histonet] RE: fine needle muscle biopsies In-Reply-To: Message-ID: _____________________________________________ From: Sharon Allen Sent: June 17, 2008 8:14 AM To: 'histonet@lists.utsouthwestern.edu' Subject: FW: fine needle muscle biopsies Hi, Can anyone doing fine needle muscle biopsies, share their method for handling these bx's i.e. method for freezing, technique for orientating, any tricks etc. I have been doing open muscle bx's for many years, but have a new neurosurgeon coming to town that wants to do this method & I would like any help I can get. Thanks in advance, Sharon sallen@hsc.mb.ca -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From SAllen <@t> exchange.hsc.mb.ca Thu Jun 19 09:40:06 2008 From: SAllen <@t> exchange.hsc.mb.ca (Sharon Allen) Date: Thu Jun 19 09:40:43 2008 Subject: [Histonet] RE: fine needle muscle biopsies In-Reply-To: Message-ID: _____________________________________________ From: Sharon Allen Sent: June 17, 2008 8:14 AM To: 'histonet@lists.utsouthwestern.edu' Subject: FW: fine needle muscle biopsies Hi, Can anyone doing fine needle muscle biopsies, share their method for handling these bx's i.e. method for freezing, technique for orientating, any tricks etc. I have been doing open muscle bx's for many years, but have a new neurosurgeon coming to town that wants to do this method & I would like any help I can get. Thanks in advance, Sharon sallen@hsc.mb.ca -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From AWeiss <@t> shorememorial.org Thu Jun 19 10:26:53 2008 From: AWeiss <@t> shorememorial.org (AWeiss@shorememorial.org) Date: Thu Jun 19 10:27:18 2008 Subject: [Histonet] Immuno's on thyroid FNA's In-Reply-To: <20080619143900.8A3F260FF72@smhex1.shorememorial.org> Message-ID: Does anyone do IM's on FNA's of thyroid and if so what panels do you use? I am trying to do this on my atypical and suspicious follicular cells to avoid further FNA's. Andrea J Weiss BST CT (ASCP) Cytotechnologist 609 653 3577 Ext 4907 aweiss@shorememorial.org From Jackie.O'Connor <@t> abbott.com Thu Jun 19 10:29:36 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Jun 19 10:30:02 2008 Subject: [Histonet] Animal Tissue processing question In-Reply-To: <2F2611250DCD6549AA3D96CE8AF1F0180112462E@giexchange.gidocs.net> Message-ID: We actually process beagle and rat tissue on the same program, mice are shorter (not their height - of course they are shorter - that's probably why they are meaner than rats - but I digress - - ). Our tissues are absolutely perfect every time, not too hard, not too soft - -kinda like the 3 bears - - yeah, I'm going on vacation tomorrow, so my brain is already in vacation mode. I'll be battling mosquitoes, pumas, and plague infested chipmunks in the Rocky Mountains. Hawaii still sounded like a better idea. Jackie O' "Rosa Fields" Sent by: histonet-bounces@lists.utsouthwestern.edu 06/19/2008 09:30 AM To "Victor Tobias" , "Rene J Buesa" cc Histonet@lists.utsouthwestern.edu Subject RE: [Histonet] Animal Tissue processing question I would have to agree with Victor, also previously from a high volume vet diagnostic lab, as a matter of practicality all of our species were also grouped onto one processor. It is very challenging to practically deal with everything one may see in a vet diagnostic lab! Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Thursday, June 19, 2008 9:01 AM To: Rene J Buesa Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Animal Tissue processing question Having worked previously in a high volume vet diagnostic lab I somewhat disagree with Rene. For optimal processing I agree, but not for practicality. On a daily basis we would have every species under the sun (almost) and they got grouped together on one processor. Some days certain tissues might be a little more difficult, but not impossible. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Rene J Buesa wrote: > You just mentioned 2 different species with peculiar characteristics. The difficulty with veterinary histology is that you will have to modify your procedures slightly to each, and you should never process tissues of different species in the same run. > From very "dry" mice tissues with less dehydration times, to pig tissues requiring more time in the antemedium because of the tisular fat (unless it is a boar) you will have to prepare modified protocol. > My advise, write down your protocols and the animals you used them with and evaluate the results in a manner that in some time you will have the protocols you need. > Sorry if this is not what you asked for, but, as I wrote you, there should be specific protocols. > Ren? J. > > histoinfo@comcast.net wrote: > Dear Histonetters, > After doing human histology for 15 years I am excited to have the opportunity to help start up a brand new research histology laboratory. It is a very small lab so I will still have my day job. But it is all new and different and a bit exciting. I would like to ask your help with the processing times. I know animal tissue is dryer so I am thinking less time in alcohol but am looking to those of you out there with the experience for a place to start when it comes to VIP processing times. I know I will be processing tissues from pigs and primates if that makes a difference. Brand new VIP, brand new embedding center, brand new microtome. . . . I can't tell you how exciting this is. Thanks for all your help. > Jennifer > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgalnow <@t> aol.com Thu Jun 19 10:47:27 2008 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Thu Jun 19 10:47:36 2008 Subject: [Histonet] milk block Message-ID: <8CAA03AE92E70E7-9C8-1505@Webmail-mg20.sim.aol.com> Does anyone know where I can get a nonRUO Milk Block and BSA block? Roxanne From algranth <@t> u.arizona.edu Thu Jun 19 11:04:55 2008 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Thu Jun 19 11:15:02 2008 Subject: [Histonet] Animal Tissue processing question In-Reply-To: References: <2F2611250DCD6549AA3D96CE8AF1F0180112462E@giexchange.gidocs.net> Message-ID: <6.2.3.4.1.20080619085804.01f01f70@algranth.inbox.email.arizona.edu> I also went from human tissue processing to animal when I came here to work. I sometimes combine different kinds of tissue and species and use a routine processing schedule but I do have special schedules for brains and embryos (mouse, chick, etc), insects, plants and very small tissues. The NSH Animal processing book is good - I'd like to see it updated with more info - and I received a wealth of information from contributors to histonet. If you want to contact me offlist about specific processing schedules please do. Andi Grantham ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From TJJ <@t> Stowers-Institute.org Thu Jun 19 11:49:14 2008 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Thu Jun 19 11:49:46 2008 Subject: [Histonet] Research Specialist (Histology Facility) job available Message-ID: Research Specialist The Stowers Institute for Medical Research has an opening for a Research Specialist to provide high quality research Histology services in the Core Facility. Responsibilities include providing molecular histology (immunohistochemistry and/or in situ hybridization) services to slide-mounted or whole mount samples; operating and maintaining histology prep and ancillary equipment; developing protocols for specialized research IHC and/or ISH related projects; and performing routine histologic techniques to include sample processing, sectioning, and/or histochemical staining. The successful candidate will be able to work independently or in a team-oriented environment; be familiar with basic biology, cell biology, or genetics; have general laboratory skills and experience to accurately record data, maintain project logs, mix solutions, and follow established safety procedures; have excellent communication skills to effectively demonstrate techniques, make presentations, conduct seminars, and/or workshops on Histology techniques. Have or develop familiarity with the appropriate literature and databases for literature searches; and possess excellent fine-motor skills for manipulation of tiny tissue samples. The minimum requirements include an undergraduate degree in biological sciences or a related field, two years experience in a research environment, and experience in molecular histology techniques (IHC and ISH) on whole mount and/or slide mounted samples. Experience in other routine and advanced histologic techniques is highly desirable. To apply visit the Stowers Institute web page at: http://www.stowers-institute.org/ScientistsSought/ScientistsSought.asp#resume Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From sprin119 <@t> umn.edu Thu Jun 19 12:36:39 2008 From: sprin119 <@t> umn.edu (Jennifer Springsteen) Date: Thu Jun 19 12:36:51 2008 Subject: [Histonet] Jr. Scientist position at U of MN Message-ID: Jr. Scientist position at U of MN: A jr. scientist position is available in the Lillehei Heart Institute Histology & Microscopy Research Facility at the University of Minnesota College of Medicine, Division of Cardiology. This is a research scientist position requiring a 4-year degree or equivalent education. Excellent opportunity to advance within the position and institute. For details and to apply, please visit the employment website: https://employment.umn.edu/applicants/jsp/shared/frameset/Frameset.jsp?time= 1213896780877 and search for job requisition number 155864. -- Jennifer L. Springsteen, B.S. Candidate for M.P.H. Lab Manager, Assistant Scientist Lillehei Heart Institute Histology & Microscopy Research Facility University of Minnesota Medical School, Division of Cardiology 4-266 Nils Hasselmo Hall 312 Church Street SE Minneapolis, MN 55455 Lab: 612-626-3090 Cell: 651-357-7916 Fax: 612-624-8118 From derek.papalegis <@t> tufts.edu Thu Jun 19 12:46:13 2008 From: derek.papalegis <@t> tufts.edu (Derek Papalegis) Date: Thu Jun 19 12:46:18 2008 Subject: [Histonet] bone perfusion Message-ID: <485A9B65.8090400@tufts.edu> Does anyone have experience with perfusion of large bones? I have an investigator that works mainly with pig jaw bones and wants to know if there is anyway to perfuse them. Regular formalin fixation has not given them the results that they need so she is looking for other ways to fix the bones. If there is no way to perfuse large bones, are there any other protocols to formalin fix these that someone can send to me? Thanks for your help Derek -- Derek Papalegis HT (ASCP) Histotechnician Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 From tim.morken <@t> thermofisher.com Thu Jun 19 12:59:58 2008 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Thu Jun 19 13:00:34 2008 Subject: [Histonet] Research Specialist (Histology Facility) job available In-Reply-To: References: Message-ID: <6BFF6D137DF6BC43B33891BA96E83B190194BEB2@PGHCR-EXMB-VS-1.na.fshrnet.com> Somebody better grab this one - this is one of the most modern and well equipped private research facilities I've ever seen. And the staff is fun too! Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Teri Sent: Thursday, June 19, 2008 9:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Research Specialist (Histology Facility) job available Research Specialist The Stowers Institute for Medical Research has an opening for a Research Specialist to provide high quality research Histology services in the Core Facility. Responsibilities include providing molecular histology (immunohistochemistry and/or in situ hybridization) services to slide-mounted or whole mount samples; operating and maintaining histology prep and ancillary equipment; developing protocols for specialized research IHC and/or ISH related projects; and performing routine histologic techniques to include sample processing, sectioning, and/or histochemical staining. The successful candidate will be able to work independently or in a team-oriented environment; be familiar with basic biology, cell biology, or genetics; have general laboratory skills and experience to accurately record data, maintain project logs, mix solutions, and follow established safety procedures; have excellent communication skills to effectively demonstrate techniques, make presentations, conduct seminars, and/or workshops on Histology techniques. Have or develop familiarity with the appropriate literature and databases for literature searches; and possess excellent fine-motor skills for manipulation of tiny tissue samples. The minimum requirements include an undergraduate degree in biological sciences or a related field, two years experience in a research environment, and experience in molecular histology techniques (IHC and ISH) on whole mount and/or slide mounted samples. Experience in other routine and advanced histologic techniques is highly desirable. To apply visit the Stowers Institute web page at: http://www.stowers-institute.org/ScientistsSought/ScientistsSought.asp#r esume Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kucak1 <@t> biognost.hr Thu Jun 19 13:47:16 2008 From: kucak1 <@t> biognost.hr (Ana Kucak) Date: Thu Jun 19 13:47:22 2008 Subject: [Histonet] Looking for an article Message-ID: <485AA9B4.80700@biognost.hr> Hi, I am looking for the following article: Scott SG, On successive double staining for histological purposes Journal of Pathology and Bacteriology 1911-1912: 16,390-8 Could someone please help me out? Thanks a lot! -- Ana Kucak Croatia e-mail: kucak1@biognost.hr From amylee779 <@t> yahoo.com Thu Jun 19 14:04:14 2008 From: amylee779 <@t> yahoo.com (Amy Lee) Date: Thu Jun 19 14:04:18 2008 Subject: [Histonet] symbols for antibody, antigen, biotin etc? Message-ID: <584102.44032.qm@web38007.mail.mud.yahoo.com> Dear histonetters, ? I am going to give a small lecture about IHC procedure. I need to make pictures including ?antigen, primary antibody, secondary antibody, DAB, etc.. So I searched? for those icons (symbols) for these antibodies etc. but couldn't find ones I?can use. ? Could anybody help me with this? Where I can find those symbols? ? Thanks in advance! ? Amy From Lynn.Burton <@t> Illinois.gov Thu Jun 19 16:13:33 2008 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Thu Jun 19 16:14:25 2008 Subject: [Histonet] Animal Tissue processing question References: <061820082013.5991.48596C7E000A512100001767221655140601000207019B9C0708@comcast.net> Message-ID: Here, at the Animal Disease Lab, in Galesburg, Illinois, we get tissues from every species one can imagine, and some you can't. We accept cases from area zoos, farms and veterinary clinics. These may be submissions of all organs or biopsies. With very few exceptions we use the following times on a Tissue Tek VIP processor: 1. formalin 30 minutes 2. formalin 30 minutes 3. 70% ETOH 30 minutes 4. 80% ETOH 30 minutes 5. 95% ETOH 30 minutes 6. 100 % ETOH 30 minutes 7. SAA 8. SAA 9. xylene or substitute, we've been using slide brite 45 minutes 10. SAA 11. paraffin 30 minutes 12. SAA 13. SAA 14. SAA We process tissues daily using a delayed start program. All tissues trimmed that day are processed together. There are some tissues which are treated specially, such as lipomas, prior to processing. Most of our tissues are mailed or shipped to us in 10% NBF and so they have been fixed adequately prior to their arrival. I hope this is of help to you. Lynn Burton Animal Disease Lab Galesburg, Il ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of histoinfo@comcast.net Sent: Wed 6/18/2008 3:13 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Animal Tissue processing question Dear Histonetters, After doing human histology for 15 years I am excited to have the opportunity to help start up a brand new research histology laboratory. It is a very small lab so I will still have my day job. But it is all new and different and a bit exciting. I would like to ask your help with the processing times. I know animal tissue is dryer so I am thinking less time in alcohol but am looking to those of you out there with the experience for a place to start when it comes to VIP processing times. I know I will be processing tissues from pigs and primates if that makes a difference. Brand new VIP, brand new embedding center, brand new microtome. . . . I can't tell you how exciting this is. Thanks for all your help. Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sharon.Genest <@t> saskatoonhealthregion.ca Thu Jun 19 17:03:20 2008 From: Sharon.Genest <@t> saskatoonhealthregion.ca (Genest, Sharon SktnHR) Date: Thu Jun 19 17:09:12 2008 Subject: [Histonet] RotoDry form Mopec Message-ID: We have just been gifted a RotoDry from Mopec which we will be using as a slide drier.Can anyone give me the temp and time that they are drying slides for routine H&E. Presently we have only small slide dryers so a few ideas on how people track the slides in and out would be helpful as well. Thanks Sharon Genest MLT Technologist Supervisor Histology Saskatoon Health Region Phone: (306)655-8197 Email: sharon.genest@saskatoonhealthregion.ca From JSCHUMA1 <@t> Fairview.org Fri Jun 20 08:10:15 2008 From: JSCHUMA1 <@t> Fairview.org (Schumacher, Jennifer J) Date: Fri Jun 20 08:10:34 2008 Subject: [Histonet] RotoDry form Mopec In-Reply-To: Message-ID: We use a large piece of filter paper, separated into 4 quadrants with a timer for each quadrant. We dry at 70 degrees celsius for 15 minutes. You may want to contact Mopec about an upgrade to the unit that they have recently discovered that is a major improvement to the longevity of the unit. It is a redesign of the blower motor box which controls the heating function. It is a great unit, in my opinion. Jennifer -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Genest, Sharon SktnHR Sent: Thursday, June 19, 2008 5:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RotoDry form Mopec We have just been gifted a RotoDry from Mopec which we will be using as a slide drier.Can anyone give me the temp and time that they are drying slides for routine H&E. Presently we have only small slide dryers so a few ideas on how people track the slides in and out would be helpful as well. Thanks Sharon Genest MLT Technologist Supervisor Histology Saskatoon Health Region Phone: (306)655-8197 Email: sharon.genest@saskatoonhealthregion.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kold <@t> neo.rr.com Fri Jun 20 10:59:07 2008 From: kold <@t> neo.rr.com (kold@neo.rr.com) Date: Fri Jun 20 10:59:12 2008 Subject: [Histonet] effect on IHC staining with the use of Cytolyt for cell blocks Message-ID: <30783016.681591213977547957.JavaMail.root@cdptpa-web27-z02> Has anybody had any problems with IHC staining on cell block slides that were processed with Cytolyt solution From lesley.bechtold <@t> jax.org Fri Jun 20 12:20:12 2008 From: lesley.bechtold <@t> jax.org (Lesley Bechtold) Date: Fri Jun 20 12:21:43 2008 Subject: [Histonet] Super spice racks?? Message-ID: <20080620132012388.00000002416@spikey> We're hoping someone out there can tell us how they solved what is probably a pretty common problem. We have three drawers full of small dye powder bottles. They're in no particular order so we waste time pulling out bottles trying to find the one we want. We think we need a super spice rack of some sort to organize these bottles. Can anyone suggest something that has worked for them? Thank you very much! Lesley Lesley S. Bechtold Senior Manager, Histopathology & Microscopy Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 From jm.lapointe <@t> accellab.com Fri Jun 20 12:30:00 2008 From: jm.lapointe <@t> accellab.com (Jean-Martin Lapointe) Date: Fri Jun 20 12:34:13 2008 Subject: [Histonet] bone perfusion References: <200806201704.m5KH4qMM011955@gateway5.lastspam.com> Message-ID: I don't think it's possible to dissect the whole jaw bone fresh and then perfuse it, since the jaw gets its blood supply from several arteries, and by dissecting the various vessels in the surrounding tissue you're making a pressure perfusion pretty much impossible. I would think that the best approach for perfusing the jaw would be to do a regional perfusion via the carotids, which will fix the whole head. Then you've got the mess of dissecting the jaw from tissue that is formalin-fixed. If they are looking for a specific site in the jaw, the other option to avoid perfusion would be to cut the segment of interest with a bone saw and fix that segment by ordinary immersion - since this opens up the bone cortex, the tissue should fix properly. __________________________________ Jean-Martin Lapointe, DMV, MS, dACVP AccelLAB Inc ? ? ------------------------------ Message: 2 Date: Thu, 19 Jun 2008 13:46:13 -0400 From: Derek Papalegis Subject: [Histonet] bone perfusion To: histonet@lists.utsouthwestern.edu Message-ID: <485A9B65.8090400@tufts.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Does anyone have experience with perfusion of large bones? I have an investigator that works mainly with pig jaw bones and wants to know if there is anyway to perfuse them. Regular formalin fixation has not given them the results that they need so she is looking for other ways to fix the bones. If there is no way to perfuse large bones, are there any other protocols to formalin fix these that someone can send to me? Thanks for your help Derek **************** From JWeems <@t> sjha.org Fri Jun 20 12:35:31 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Jun 20 12:35:41 2008 Subject: [Histonet] Super spice racks?? In-Reply-To: <20080620132012388.00000002416@spikey> References: <20080620132012388.00000002416@spikey> Message-ID: <982A0A9461F9BF438C7B19A6E425A383246511@ITSSSXM01V6.one.ads.che.org> I would put dividers (cardboard would work) and alphabetize them. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lesley Bechtold Sent: Friday, June 20, 2008 1:20 PM To: Histology Network Subject: [Histonet] Super spice racks?? We're hoping someone out there can tell us how they solved what is probably a pretty common problem. We have three drawers full of small dye powder bottles. They're in no particular order so we waste time pulling out bottles trying to find the one we want. We think we need a super spice rack of some sort to organize these bottles. Can anyone suggest something that has worked for them? Thank you very much! Lesley Lesley S. Bechtold Senior Manager, Histopathology & Microscopy Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From MITCHELLJA <@t> email.chop.edu Fri Jun 20 12:35:15 2008 From: MITCHELLJA <@t> email.chop.edu (Janice Mitchell) Date: Fri Jun 20 12:35:46 2008 Subject: [Histonet] Recycling Xylene Message-ID: Good Friday Afternoon All, Our envirnonmental health and safety department is looking into recycling xylene and alcohol. Anyone out there with any input on brands of recycling equipment, ease of use, quality of recycled chemicals, etc... Thanks, Janice From KHilburn <@t> compucyte.com Fri Jun 20 12:39:53 2008 From: KHilburn <@t> compucyte.com (Kate Hilburn) Date: Fri Jun 20 12:39:39 2008 Subject: [Histonet] Quantitative Imaging Cytometry Workshop--June 25 in San Francisco Message-ID: CompuCyte Corporation will be offering a Technology Workshop, "Quantitative Imaging Cytometry: Technology to Support the New Role of Pathology in the Era of Specific Targeted Therapies" next Wednesday, June 25 at noon, at the Hyatt Regency Hotel in San Francisco. The presentation will highlight the technology and methods for extracting high-content data and images from cellular, tissue and tissue microarray specimens labeled with fluorescent and chromatic dyes. The workshop is held in conjunction with the Annual Meeting of the Society of Toxicologic Pathology, but you do not need to be registered for the meeting in order to attend. Lunch will be provided; however, seating is limited. If you are attending the STP meeting or are in the area, and you would like to attend, please email your registration request to khilburn@compucyte.com, providing your name, affiliation, and contact information. We will add you to the registration list. Kate Hilburn CompuCyte Corporation From histoinfo <@t> comcast.net Fri Jun 20 13:32:37 2008 From: histoinfo <@t> comcast.net (histoinfo@comcast.net) Date: Fri Jun 20 13:32:46 2008 Subject: [Histonet] Super spice racks?? Message-ID: <062020081832.2344.485BF7C4000D181500000928221559341401000207019B9C0708@comcast.net> I agree with Joyce but I would add making a master list after they are alphabetized listing them in numerical order. Then use round sticky dots that you can write the corresponding numbers on. Put the dots on the top of the bottles and you can open the drawer and find what you need almost instantly. You can even color code the dots if you want to. Jennifer Saunders H.T.(ASCP) -------------- Original message -------------- From: "Weems, Joyce" > I would put dividers (cardboard would work) and alphabetize them. > From rjbuesa <@t> yahoo.com Fri Jun 20 13:46:41 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 20 13:46:46 2008 Subject: [Histonet] Super spice racks?? In-Reply-To: <20080620132012388.00000002416@spikey> Message-ID: <963222.94286.qm@web65706.mail.ac4.yahoo.com> I just placed a round label on top of the cap and could read the name without having to pull the bottle out. Ren? J. Lesley Bechtold wrote: We're hoping someone out there can tell us how they solved what is probably a pretty common problem. We have three drawers full of small dye powder bottles. They're in no particular order so we waste time pulling out bottles trying to find the one we want. We think we need a super spice rack of some sort to organize these bottles. Can anyone suggest something that has worked for them? Thank you very much! Lesley Lesley S. Bechtold Senior Manager, Histopathology & Microscopy Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Fri Jun 20 13:55:27 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Jun 20 13:55:39 2008 Subject: [Histonet] Super spice racks?? In-Reply-To: <062020081832.2344.485BF7C4000D181500000928221559341401000207019B9C0708@comcast.net> Message-ID: <000701c8d307$383ddfb0$d00f7ca5@lurie.northwestern.edu> We do that with our antibodies and have the list on the refrigerator door. We found a box that was divided- maybe held small vials. We've also got lesser used on in freezer boxes that would hold cryovials. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histoinfo@comcast.net Sent: Friday, June 20, 2008 1:33 PM To: Weems, Joyce; Lesley Bechtold; Histology Network Subject: RE: [Histonet] Super spice racks?? I agree with Joyce but I would add making a master list after they are alphabetized listing them in numerical order. Then use round sticky dots that you can write the corresponding numbers on. Put the dots on the top of the bottles and you can open the drawer and find what you need almost instantly. You can even color code the dots if you want to. Jennifer Saunders H.T.(ASCP) -------------- Original message -------------- From: "Weems, Joyce" > I would put dividers (cardboard would work) and alphabetize them. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maxim_71 <@t> mail.ru Fri Jun 20 14:56:02 2008 From: Maxim_71 <@t> mail.ru (Maxim_71@mail.ru) Date: Fri Jun 20 14:59:16 2008 Subject: [Histonet] symbols for antibody, antigen, biotin etc? Message-ID: <1605125020.20080620235602@mail.ru> Amy: On the website www.dako.com/uk I found excellent guide: "Immunohistochemical Staining Methods" {Fourth Edition). You can find here all IHC symbols. This giude you can also on the website www.ihcworld. Sorry, but I can not remember full web-references. Please try "search" in these websites. Maxim Peshkov Russia Taganrog. -- ? ?????????, Maxim mailto:Maxim_71@mail.ru From Simoskevitz <@t> Osteotech.com Fri Jun 20 15:02:12 2008 From: Simoskevitz <@t> Osteotech.com (Simoskevitz@Osteotech.com) Date: Fri Jun 20 15:02:34 2008 Subject: [Histonet] Ricki Simoskevitz is out of the office. Message-ID: I will be out of the office starting 06/20/2008 and will not return until 06/30/2008. I will respond to your message when I return. From rjbuesa <@t> yahoo.com Fri Jun 20 15:24:11 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 20 15:24:15 2008 Subject: [Histonet] Recycling Xylene In-Reply-To: Message-ID: <756760.24502.qm@web65711.mail.ac4.yahoo.com> I used a B/R Corporation recycler and it paid itself recycling xylene in 2 years. It is not profitable to recycle ethanol, it takes twice the time and costs less than half. Recycling xylene is a good idea. Ren? J. Janice Mitchell wrote: Good Friday Afternoon All, Our envirnonmental health and safety department is looking into recycling xylene and alcohol. Anyone out there with any input on brands of recycling equipment, ease of use, quality of recycled chemicals, etc... Thanks, Janice _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rchiovetti <@t> yahoo.com Fri Jun 20 15:34:35 2008 From: rchiovetti <@t> yahoo.com (Robert Chiovetti) Date: Fri Jun 20 15:34:39 2008 Subject: [Histonet] Super spice racks?? Message-ID: <821513.42622.qm@web58902.mail.re1.yahoo.com> Leslie, Good idea about putting labels on the caps of the bottles! Although more $$ would be involved, it might be worth investing in something like a cytec storage cabinet. The shelves are clear plastic w/ holes in them to hold small bottles, and the door is clear plastic so you can see through it. Take a look at the bottom of this web page from Hacker Instruments: Cheers, Bob Robert (Bob) Chiovetti, Ph.D. Southwest Precision Instruments www.swpinet.com See What's New on Our Website! Arizona's Microscopy Resource 132 North Elster Drive Tucson, AZ 85710-3212 Tel./Fax 520-546-4986 Member, Arizona Small Business Association (www.asba.com) ----- Original Message ---- From: Lesley Bechtold To: Histology Network Sent: Friday, June 20, 2008 10:20:12 AM Subject: [Histonet] Super spice racks?? We're hoping someone out there can tell us how they solved what is probably a pretty common problem. We have three drawers full of small dye powder bottles. They're in no particular order so we waste time pulling out bottles trying to find the one we want. We think we need a super spice rack of some sort to organize these bottles. Can anyone suggest something that has worked for them? Thank you very much! Lesley Lesley S. Bechtold Senior Manager, Histopathology & Microscopy Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amber.mckenzie <@t> gastrodocs.net Fri Jun 20 16:04:09 2008 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Fri Jun 20 16:04:14 2008 Subject: [Histonet] HTL study guides In-Reply-To: <484EB80E.8090405@shaw.ca> Message-ID: <03C921A1EAF7F541B16543F6EC6A4B3701C31901@giamail2.Gia.com> Does anyone know what the best way to study/pass the HTL is? I've seen all kinds of online classes and work shops for the HT, but nothing for the HTL. Thanks, Amber McKenzie, B.S., HT (ASCP) From tim.morken <@t> thermofisher.com Fri Jun 20 16:34:48 2008 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Fri Jun 20 16:35:15 2008 Subject: [Histonet] HTL study guides In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B3701C31901@giamail2.Gia.com> References: <484EB80E.8090405@shaw.ca> <03C921A1EAF7F541B16543F6EC6A4B3701C31901@giamail2.Gia.com> Message-ID: <6BFF6D137DF6BC43B33891BA96E83B190194C361@PGHCR-EXMB-VS-1.na.fshrnet.com> Amber, it's a tough job since you have to be ready for anything. When I did it back in the late '80's we had a study group of 4 people and we met once a week and just went through all of Sheehan (Carson's book was not out yet) outling it and learning essentially everything in it. We made charts of all the fixatives, variations on all the stains, reasons for using each, result, etc. We went through all the NSH study guides several times. It took about a long time. It helped a lot that I worked in a lab that did almost everything in the book - even EM - so we could try things out first hand. We spent about a year preparing for the HT first and then two years later took the HTL. All of us passed. I felt it was equivilent to a masters degree program. Tim Morken Technical Support Manager Lab Vision Products Anatomical Pathology ThermoFisher Scientific -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Friday, June 20, 2008 2:04 PM To: HistoNet Server Subject: [Histonet] HTL study guides Does anyone know what the best way to study/pass the HTL is? I've seen all kinds of online classes and work shops for the HT, but nothing for the HTL. Thanks, Amber McKenzie, B.S., HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histology.bc <@t> shaw.ca Fri Jun 20 18:41:24 2008 From: histology.bc <@t> shaw.ca (Paul Bradbury) Date: Fri Jun 20 18:41:57 2008 Subject: [Histonet] Super spice racks?? In-Reply-To: <20080620132012388.00000002416@spikey> References: <20080620132012388.00000002416@spikey> Message-ID: <485C4024.5010608@shaw.ca> Our solution to this same problem is: Give each product a code number (acid fuchsin is A-1, Acridine Orange is A-2, Light Green SF Yellowish is L-4, etc). Create a master list of all the dry dye powders and their alloted code number. Stick a label with the code number on the lid of each bottle. File the dye bottles in drawers with dividers (A - C, D - F, G - I, etc). Cardboard dividers held in place with duct tape work just fine. To find a specific dye, check the master sheet, scan the labels in the appropriate section of the drawer ... and voila ! It's easy, it's cheap and it works! Paul Kamloops, Canada Lesley Bechtold wrote: > We're hoping someone out there can tell us how they solved what is probably a pretty common problem. We have three drawers full of small dye powder bottles. They're in no particular order so we waste time pulling out bottles trying to find the one we want. We think we need a super spice rack of some sort to organize these bottles. Can anyone suggest something that has worked for them? Thank you very much! > > Lesley > > > Lesley S. Bechtold > Senior Manager, Histopathology & Microscopy Sciences > The Jackson Laboratory > 600 Main St. > Bar Harbor, ME 04609 > 207-288-6322 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From raj <@t> bluemarble.net Fri Jun 20 21:41:07 2008 From: raj <@t> bluemarble.net (Rebecca Johnson) Date: Fri Jun 20 21:41:08 2008 Subject: [Histonet] Cassette Printer Message-ID: <00a301c85bdf$68294970$7b48f9d8@CHURCH> We are getting new AP computer system PowerPath, what cassette printer are people using with this system? Thanks Becky From jmjohnson34 <@t> hotmail.com Sat Jun 21 13:44:39 2008 From: jmjohnson34 <@t> hotmail.com (Jennifer Johnson) Date: Sat Jun 21 13:44:42 2008 Subject: [Histonet] Re: HTL exam Message-ID: Amber, I got my Bachelor's Degree in Biology (majored in Botany) and after reading Freida Carson's Book and using her study guide, I passed the HTL on my first try. I have Sheehan's, AFIP, and Carson's manuals at work but feel most comfortable with Carson's ease of use and explanations to lab math! I have a study guide that I made myself on a disk somewhere. I will try to locate it and forward it to you. Good Luck! Jennifer Johnson, HTL (ASCP) _________________________________________________________________ Earn cashback on your purchases with Live Search - the search that pays you back! http://search.live.com/cashback/?&pkw=form=MIJAAF/publ=HMTGL/crea=earncashback From AnthonyH <@t> chw.edu.au Sun Jun 22 18:12:31 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Jun 22 18:12:46 2008 Subject: [Histonet] effect on IHC staining with the use of Cytolyt for cellblocks In-Reply-To: <30783016.681591213977547957.JavaMail.root@cdptpa-web27-z02> Message-ID: Remember the cytolyte solution is an alcohol-based fixation. Very little formalin, so enzyme pre-treatments will tend to chew up the sections. Try the IPXs leaving out any enzyme unmasking. Some antibodies may not recognise their antigens (eg p53) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kold@neo.rr.com Sent: Saturday, 21 June 2008 1:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] effect on IHC staining with the use of Cytolyt for cellblocks Has anybody had any problems with IHC staining on cell block slides that were processed with Cytolyt solution _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From renafail <@t> bellsouth.net Sun Jun 22 19:46:41 2008 From: renafail <@t> bellsouth.net (Rena Fail) Date: Sun Jun 22 19:46:43 2008 Subject: [Histonet] Super spice racks?? In-Reply-To: <485C4024.5010608@shaw.ca> Message-ID: <000001c8ed26$6eda0060$0301a8c0@RENAD4YK9B8ABE> Personally, I like the expanding tier shelves for spices. Rena Fail -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paul Bradbury Sent: Friday, June 20, 2008 7:41 PM To: Lesley Bechtold; HistoNet Server Subject: Re: [Histonet] Super spice racks?? Our solution to this same problem is: Give each product a code number (acid fuchsin is A-1, Acridine Orange is A-2, Light Green SF Yellowish is L-4, etc). Create a master list of all the dry dye powders and their alloted code number. Stick a label with the code number on the lid of each bottle. File the dye bottles in drawers with dividers (A - C, D - F, G - I, etc). Cardboard dividers held in place with duct tape work just fine. To find a specific dye, check the master sheet, scan the labels in the appropriate section of the drawer ... and voila ! It's easy, it's cheap and it works! Paul Kamloops, Canada Lesley Bechtold wrote: > We're hoping someone out there can tell us how they solved what is probably a pretty common problem. We have three drawers full of small dye powder bottles. They're in no particular order so we waste time pulling out bottles trying to find the one we want. We think we need a super spice rack of some sort to organize these bottles. Can anyone suggest something that has worked for them? Thank you very much! > > Lesley > > > Lesley S. Bechtold > Senior Manager, Histopathology & Microscopy Sciences > The Jackson Laboratory > 600 Main St. > Bar Harbor, ME 04609 > 207-288-6322 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From immunos4valerie <@t> yahoo.com Mon Jun 23 03:57:12 2008 From: immunos4valerie <@t> yahoo.com (VALERIE KENNEDY) Date: Mon Jun 23 03:57:23 2008 Subject: [Histonet] HTL study guides In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B3701C31901@giamail2.Gia.com> Message-ID: <453019.46582.qm@web35407.mail.mud.yahoo.com> Amber, NSH has an excellent study guide on CD that has all 14 of their books and it covers what is required for the HTL.? Also ASCP has online testing that you can take AFTER you have read Freda Carson from cover to cover.? I took the HTL in October 2007.? I must say, the questions are geared to really make sure you actually do the different techniques in the laboratory therefore making? up for not having to take the practical, which I considered the fun part when I took the HT in 1992.? If you happen to need any study guides I am certainly send them your way. Valerie Kennedy MHA, HTL(ASCP)HT,QIHC --- On Fri, 6/20/08, Amber McKenzie wrote: From: Amber McKenzie Subject: [Histonet] HTL study guides To: "HistoNet Server" Date: Friday, June 20, 2008, 5:04 PM Does anyone know what the best way to study/pass the HTL is? I've seen all kinds of online classes and work shops for the HT, but nothing for the HTL. Thanks, Amber McKenzie, B.S., HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Mon Jun 23 05:58:00 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Jun 23 05:57:48 2008 Subject: [Histonet] Austin TX Message-ID: <000801c8d520$01429670$0302a8c0@yourxhtr8hvc4p> I would like to have some techs from Austin contact me at jnocito@satx.rr.com Some of the email addresses from my membership list are incorrect. This concerns next year's TSH meeting. Thanks JTT From kmerriam2003 <@t> yahoo.com Mon Jun 23 08:59:43 2008 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Mon Jun 23 08:59:48 2008 Subject: [Histonet] DAKO FISH pretreatment buffer (MES) Message-ID: <742043.77284.qm@web50302.mail.re2.yahoo.com> Hello, This might seem to?be a strange question, but I have been working up my FFPE FISH?procedure with the DAKO?FISH Histology Kit (I have had some nice preliminary results).? I have been noticing a strange greasy film on?my slides that seems to be occuring during the heat pretreatment step (DAKO pretreatment buffer, which is MES; a very?long name and a buffer I have not used until now); there is also a?greasy film on the top of the buffer solution. Is this?common?? Can it hurt my slides?? I did manage to remove it?by dehydrating my slides through 95, 100 and?then clearing in xylene.? I then rehydrated them and continued my?FISH procedure, I am in the middle of staining, so I won't know if it worked until tomorrow.? I am not even sure if I should continue and add my probe, which is hard to come by. You might be thinking that there may still be wax on my slides, I thought of that too, but I deparaffinized the same as I always do for IHC, with 3 (3-minute) changes in xylene, 2 (3-minute) changes of 100% and then a change each of 95% and 80% before hydration in water.? I have never had this problem with my IHC staining or anything else, so I am inclined to think that it is the MES buffer that has some type of greasy component to it. What does everyone think? ?Kim Merriam, MA, HT(ASCP) Cambridge, MA From JMyers1 <@t> aol.com Mon Jun 23 10:03:39 2008 From: JMyers1 <@t> aol.com (JMyers1@aol.com) Date: Mon Jun 23 10:03:50 2008 Subject: [Histonet] IHC staining of previously H&E-stained prostate cores Message-ID: I'm trying to stain previously H&E-stained prostate core biopsies for HMW-CK and P504S, and I've been obtaining less than ideal results. I suspect that this might be caused by inadequate removal of mounting media (once the tape-coverslip is removed with acetone) or over/under- retrieval, but I could really use some advice from someone who has already worked through a situation like this. **************Gas prices getting you down? Search AOL Autos for fuel-efficient used cars. (http://autos.aol.com/used?ncid=aolaut00050000000007) From LSebree <@t> uwhealth.org Mon Jun 23 10:47:27 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Mon Jun 23 10:47:38 2008 Subject: [Histonet] IHC staining of previously H&E-stained prostate cores In-Reply-To: Message-ID: We haven't specifically restained prostate bxs for IHC but we have IHC stained over H&E staining on other tissues. We remove the coverslip tape with a 50/50 xylene/acetone mix, then run down through xylene and graded alcohols to remove any remaining mounting media and hydrate to water. From here we use our usual protocol minus any deparaffinization step. This has always worked fine for us unless the original H&E slide was not a "+" slide in which case we sometimes lose tissue. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JMyers1@aol.com Sent: Monday, June 23, 2008 10:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC staining of previously H&E-stained prostate cores I'm trying to stain previously H&E-stained prostate core biopsies for HMW-CK and P504S, and I've been obtaining less than ideal results. I suspect that this might be caused by inadequate removal of mounting media (once the tape-coverslip is removed with acetone) or over/under- retrieval, but I could really use some advice from someone who has already worked through a situation like this. **************Gas prices getting you down? Search AOL Autos for fuel-efficient used cars. (http://autos.aol.com/used?ncid=aolaut00050000000007) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgalnow <@t> aol.com Mon Jun 23 10:47:46 2008 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Mon Jun 23 10:47:55 2008 Subject: [Histonet] IHC staining of previously H&E-stained prostate cores In-Reply-To: References: Message-ID: <8CAA35F9DD7AD06-15C4-DBC@webmail-de03.sysops.aol.com> Joe, When we have to do this, we let the slides soak in xylene until the cover slip fails off.? We then, run a de-wax program for the slides to hydrate them and then treat them like any other IHC.? You may or may not need to counter stain them when they are finished.? Should work great. Roxanne -----Original Message----- From: JMyers1@aol.com To: histonet@lists.utsouthwestern.edu Sent: Mon, 23 Jun 2008 11:03 am Subject: [Histonet] IHC staining of previously H&E-stained prostate cores I'm trying to stain previously H&E-stained prostate core biopsies for HMW-CK and P504S, and I've been obtaining less than ideal results. I suspect that this might be caused by inadequate removal of mounting media (once the tape-coverslip is removed with acetone) or over/under- retrieval, but I could really use some advice from someone who has already worked through a situation like this. **************Gas prices getting you down? Search AOL Autos for fuel-efficient used cars. (http://autos.aol.com/used?ncid=aolaut00050000000007) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kimtournear <@t> yahoo.com Mon Jun 23 11:54:09 2008 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Mon Jun 23 11:54:12 2008 Subject: [Histonet] Tissue-Tek Xpress x50 Message-ID: <502440.73722.qm@web50310.mail.re2.yahoo.com> Hi, Is anyone using the Tissue-Tek Xpress x50 out there? Do you like it? Can you do traditional overnight processing or is it only for?rapid processing? Any feed back would be helpful... Thanks, ~Kim ~ Tucson Medical Center Tucson, AZ ? ~Don't?let your life end before it begins~ ? OU Rocks!!!! From jqb7 <@t> cdc.gov Mon Jun 23 12:02:30 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Mon Jun 23 12:03:22 2008 Subject: [Histonet] Tissue-Tek Xpress x50 In-Reply-To: <502440.73722.qm@web50310.mail.re2.yahoo.com> References: <502440.73722.qm@web50310.mail.re2.yahoo.com> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A7F23BEC@LTA3VS011.ees.hhs.gov> Kim, I have the larger version of the Xpress X50. It is great but it is a microwave processor and as such you do not use it as a traditional overnight processor. Email me directly if you have specific questions and/or concerns. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Tournear Sent: Monday, June 23, 2008 12:54 PM To: Histonet Subject: [Histonet] Tissue-Tek Xpress x50 Hi, Is anyone using the Tissue-Tek Xpress x50 out there? Do you like it? Can you do traditional overnight processing or is it only for?rapid processing? Any feed back would be helpful... Thanks, ~Kim ~ Tucson Medical Center Tucson, AZ ? ~Don't?let your life end before it begins~ ? OU Rocks!!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Jun 23 12:07:24 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jun 23 12:07:28 2008 Subject: [Histonet] Tissue-Tek Xpress x50 In-Reply-To: <502440.73722.qm@web50310.mail.re2.yahoo.com> Message-ID: <519433.99746.qm@web65714.mail.ac4.yahoo.com> Both Xpress models have been engineered to process in a continuous flow of cassettes not for overnight (delayed) processing. Perhaps you could leave the cassettes in the last retort, but I am not sure about that nor it is recommended, and it would defeat the design of the instrument. Ren? J. Kim Tournear wrote: Hi, Is anyone using the Tissue-Tek Xpress x50 out there? Do you like it? Can you do traditional overnight processing or is it only for rapid processing? Any feed back would be helpful... Thanks, ~Kim ~ Tucson Medical Center Tucson, AZ ~Don't let your life end before it begins~ OU Rocks!!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Jun 23 12:07:38 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jun 23 12:07:42 2008 Subject: [Histonet] Tissue-Tek Xpress x50 In-Reply-To: <502440.73722.qm@web50310.mail.re2.yahoo.com> Message-ID: <805464.47404.qm@web65715.mail.ac4.yahoo.com> Both Xpress models have been engineered to process in a continuous flow of cassettes not for overnight (delayed) processing. Perhaps you could leave the cassettes in the last retort, but I am not sure about that nor it is recommended, and it would defeat the design of the instrument. Ren? J. Kim Tournear wrote: Hi, Is anyone using the Tissue-Tek Xpress x50 out there? Do you like it? Can you do traditional overnight processing or is it only for rapid processing? Any feed back would be helpful... Thanks, ~Kim ~ Tucson Medical Center Tucson, AZ ~Don't let your life end before it begins~ OU Rocks!!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Mon Jun 23 12:10:59 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Mon Jun 23 12:11:22 2008 Subject: [Histonet] Tissue-Tek Xpress x50 In-Reply-To: <519433.99746.qm@web65714.mail.ac4.yahoo.com> References: <502440.73722.qm@web50310.mail.re2.yahoo.com> <519433.99746.qm@web65714.mail.ac4.yahoo.com> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A7F23BEF@LTA3VS011.ees.hhs.gov> The last retort is a heated, empty "bath"........you should NOT leave the tissue there for very long at all. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, June 23, 2008 1:07 PM To: kimtournear@yahoo.com; Histonet Subject: Re: [Histonet] Tissue-Tek Xpress x50 Both Xpress models have been engineered to process in a continuous flow of cassettes not for overnight (delayed) processing. Perhaps you could leave the cassettes in the last retort, but I am not sure about that nor it is recommended, and it would defeat the design of the instrument. Ren? J. Kim Tournear wrote: Hi, Is anyone using the Tissue-Tek Xpress x50 out there? Do you like it? Can you do traditional overnight processing or is it only for rapid processing? Any feed back would be helpful... Thanks, ~Kim ~ Tucson Medical Center Tucson, AZ ~Don't let your life end before it begins~ OU Rocks!!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amber.mckenzie <@t> gastrodocs.net Mon Jun 23 13:33:33 2008 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Mon Jun 23 13:33:42 2008 Subject: [Histonet] Advice on Training Histology Lab Assistants In-Reply-To: <484EB80E.8090405@shaw.ca> Message-ID: <03C921A1EAF7F541B16543F6EC6A4B3701C31A87@giamail2.Gia.com> Question about the training of lab assistants into Histology: How do you decide if want/need to train someone on our field? Do you ever have a "feeling" that your lab assistant wouldn't be a good candidate for histology? That maybe she wants to do it for the money and not the love of Histology? And, do you train on your own time vs in your spare time? Do you charge your company extra for being a teacher or do it out of the goodness of your heart? My situation is that I have a lab assistant who does the accessioning, grossing of GI's, and types the gross description/clinical history. She watches/observes me when she has down time and now she thinks that it's an easy job...she can do it...wants to make more money (she has researched Histology on the internet) and doesn't want to be a lab assistant her whole life (can't blame her). And, she has a B.S. in Business so no background in biology. She has plans to take online classes to get her HT certification and I guess (although she hasn't discussed it with me) she'll want me to train her in the lab to do the cutting and staining. How do you train someone in Histo when they have another job to do or the fact that you don't need another HT in your lab? So, if I train her and she gets certified and we have no openings, then she just quits and finds an HT job elsewhere and I lose a lab assistant? I know lots of question, but I've been thinking a lot about this situation and can't decide what to do. Thanks, Amber McKenzie, BS, HT (ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Histonet Sent: Tuesday, June 10, 2008 12:21 PM To: McKnight, Tanisha; HistoNet Server Subject: Re: [Histonet] Advice on Training Histology Lab Assistants Hi Tanisha, Here in Canada, Medical Laboratory Assistants have been used as a vital part of the laboratory workforce for many years. The national certifying body, the Canadian Society for Medical Laboratory Sciences, has adopted the MLA group and has established a National Syllabus of Studies and required competencies for each laboratory discipline. By standardizing the required competencies and the training of MLA's, it is possible to clearly define the precise role and duties that an MLA is permitted to perform. The duties that MLA's perform have been specifically chosen to avoid any erosion of responsibilities from the technologist's duties. In practice, certified technologists can perform any and all duties in the laboratory, whereas MLA's may perform only specific tasks. MLA's are paid on a lower scale than a certified technologist to reflect their more limited responsibilities. The separation of technologist's and assistant's duties is vital, especially in a situation where employers may be tempted to employ the less expensive of the two groups in order to cut their costs. Medical Laboratory Assistants are permitted to work in the gross room, accessioning specimens, entering data into the computer, labeling cassettes, filing and retrieving specimens, filing and retrieving blocks. The assistants also maintain the tissue processors and fluid levels. However, gross descriptions, specimen selection and dissection are the sole responsibility of the technologists. Embedding, sectioning, frozen sections, and staining are also the responsibility of the technologists. By assigning the more mundane, but still critical, tasks to less qualified personnel, the technologists are available to concentrate on the more demanding procedures. This system has worked very well and very effectively for many years. There is a distance education course for Histology Assistants offered through the Open Learning Division of Thompson Rivers University of British Columbia. This is specifically written for workers who have no prior knowledge of histology. It focuses on "pre-analytical procedures" but also provides a good deal of background information on diagnostic histopathology, tissues, fixation, tissues types, common specimens, tissue processing, filing, storage. It also covers some aspects of specimen preparation for cytology specimens, fluids, aspirates, etc. http://www.tru.ca/distance/programs/health_sci/medlabasst/courses.html#m lap161 The Open Learning course is available to anyone, anywhere, and may be completed within a very flexible time frame. The current cost of the course is $400.00, including all course materials, examinations, on-line tutors, and toll-free tutor phone calls. Paul Bradbury, Kamloops, Canada ************************************************************************ ************************************* McKnight, Tanisha wrote: > Hello All: > > I have a few people, now working in accessioning, who are interested in > working in Histology. I am thinking of potentially creating "Histology > Lab Assistant" positions to help them transition. They have already been > told that they will need to go through an accredited program to become > full Techs. We have one here in Indiana that I went through and it is > great. > > Can you all share your strategies for training? How do you separate what > Assistants are allowed to do from what Techs do? What regulatory > guidelines do you follow if any when deciding? > > I was thinking of training them to embed and create sections first (on > limited specimen types). I would not allow certain biopsies or really > small specimens. Under regulations, would sectioning and embedding be > considered "testing"? > > Any help or advice would be appreciated. > > Tanisha N. McKnight, HT (ASCP) > Covance CLS Indianapolis > Specimen Management, Anatomic Pathology > > > > > ----------------------------------------------------- > Confidentiality Notice: This e-mail transmission > may contain confidential or legally privileged > information that is intended only for the individual > or entity named in the e-mail address. If you are not > the intended recipient, you are hereby notified that > any disclosure, copying, distribution, or reliance > upon the contents of this e-mail is strictly prohibited. > > If you have received this e-mail transmission in error, > please reply to the sender, so that we can arrange > for proper delivery, and then please delete the message > from your inbox. Thank you. > > > ------------------------------------------------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Mon Jun 23 14:19:06 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Jun 23 14:19:14 2008 Subject: [Histonet] formalin formula Message-ID: Hi, I am looking for the origin of our formalin-formula. Perhaps someone out there has a similar recept or knows the reference. 8% buffered formaldehyd: 200 ml 36-40% Formaldehyd + 200 ml 0,0342 M Phosphatbuffer pH 7,8 + 600 ml Aqua dest.; pH = 7,4 Thanks in advance Gudrun Lang From pruegg <@t> ihctech.net Mon Jun 23 14:25:53 2008 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Mon Jun 23 14:26:02 2008 Subject: [Histonet] dcadorette-HallIHCRG Membership Application Form Message-ID: <661957CAEF8641EDBC6CB99D092B9616@ihctechq9h2qof> Verify NSH membership please Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 email pruegg@ihctech.net website www.ihctech.net IHC Resource Group www.ihcrg.org _____ From: webmaster@neo.agsci.colostate.edu [mailto:webmaster@neo.agsci.colostate.edu] Sent: Monday, June 23, 2008 12:29 PM To: patsy.ruegg@gmail.com Subject: IHCRG Membership Application Form _____ New_Application: Yes Last_Name: Cadorette-Hall First_Name: Diane NSH_Member: Yes Employer: InCyte Pathology Laboratory Address: 13103 E. Mansfield Avenue City: Spokane State: WA Zip: 99216 Province: Country: Phone: 509-892-2744 Ext: Fax: Email: DHall@incytepathology.com Surgical_Pathology: Yes Hematopathology: Orthopedic: Veterinary: Immunology: Plastics: Research: Paraffin: Yes Frozen: Yes Cytospins: Smears_Touch_Preps: Yes Plastics_Sample: Sample_Other: PAP: APAAP: ABC_HRP: ABC_AP: SA_HRP: SA_AP: IF: IHC_Other: ISH: Gels: PCR: Mol_Other: Automated_IHC: No Company: Select from list IHC_Qualification: No Date_Taken_Exam: Like_To_Take_Exam: No Expected_Date: Can_Provide_Tissues: No Tissues_Provided: Need_Tissues: No Tissue_Needed: Remote Name: 66.225.3.246 HTTP User Agent: Mozilla/4.0 (compatible; MSIE 7.0; Windows NT 5.1; FunWebProducts; .NET CLR 1.1.4322; .NET CLR 1.0.3705) Date: 06/23/2008 Time: 12:28 PM Other_Tech From stephanie.d.rivera <@t> gsk.com Mon Jun 23 15:14:28 2008 From: stephanie.d.rivera <@t> gsk.com (stephanie.d.rivera@gsk.com) Date: Mon Jun 23 15:14:47 2008 Subject: [Histonet] Animal Tissue processing question In-Reply-To: <061820082013.5991.48596C7E000A512100001767221655140601000207019B9C0708@comcast.net> Message-ID: Try this protocol and adjust to your lab. See how the microtomy is for you. Rats, Dog,Primate,Pig all gets processed on the same protocol. 40 min in all solutions +parrafin.(Formalin, 70, 80,95,95,100,100,100, xylene 3 changes). Including eyes. Dog &primate eyes are fixed in 3% gluteraldehdye then transferred to 10%formalin. All Testes and Rodent eyes start in 70% alcohol,80,95,95,100,100,100, xylene (3)changes. Fix testes in bouins for 24-48 hrs then transfer to 70%alcohol and process from there. Stay consistent with fixation time. Dog and primate brains process from 70% alcohol for 90minutes in all reagents on different protocol. Brains and Femoral head fixed in 30 % formalin. 24 hrs then tranfer to 10% formalin. Bones get decal. Some labs fix everything in 10% Formalin. All mice organs are processed on different protocol. 30 minutes in all reagents start with formalin. Eyes start in 70%. Stephanie D. Rivera Safety Assessment Department GlaxoSmithKline 709 Swedeland RD King of Prussia, PA 19406 phone: 610-270-7340 fax: 610-270-7202 histoinfo@comcast.net Sent by: histonet-bounces@lists.utsouthwestern.edu 18-Jun-2008 16:13 To Histonet@lists.utsouthwestern.edu cc Subject [Histonet] Animal Tissue processing question Dear Histonetters, After doing human histology for 15 years I am excited to have the opportunity to help start up a brand new research histology laboratory. It is a very small lab so I will still have my day job. But it is all new and different and a bit exciting. I would like to ask your help with the processing times. I know animal tissue is dryer so I am thinking less time in alcohol but am looking to those of you out there with the experience for a place to start when it comes to VIP processing times. I know I will be processing tissues from pigs and primates if that makes a difference. Brand new VIP, brand new embedding center, brand new microtome. . . . I can't tell you how exciting this is. Thanks for all your help. Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Mon Jun 23 16:31:12 2008 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Mon Jun 23 16:31:22 2008 Subject: [Histonet] pardon me Message-ID: <5507ABE28A7447DAAE803B7C4C31559B@ihctechq9h2qof> Please disregard the message from Patsy asking for NSH membership verification, I meant to send it to NSH and hit the histonet button by mistake. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 email pruegg@ihctech.net website www.ihctech.net IHC Resource Group www.ihcrg.org From tjasper <@t> copc.net Mon Jun 23 19:22:41 2008 From: tjasper <@t> copc.net (Thomas Jasper) Date: Mon Jun 23 19:22:47 2008 Subject: [Histonet] IHC staining of previously H&E-stained prostate cores References: Message-ID: <90354A475B420441B2A0396E5008D4965E20E9@copc-sbs.COPC.local> Just a thought...it might be the fact that the plastic coverslip is removed by soaking in acetone (your only choice). I know that we've had a similar experience here. The situation - our previous prostate needle double stain (p504s and HMW-CK) used to have to be done as 2 separate runs. This is no longer the case, however, when it was, folks would occasionally forget to hold over the slides that were to receive the next stain. These of course would then be coverslipped. We would remove the coverslips (acetone) and run them back to water, then rerun for the second Ab. Every time this happened the 2nd Ab never worked. By process of elimination, we've deduced that the acetone exposure was the limiting factor. I'm not a chemical engineer, but I believe that was our problem and it may be your problem as well. I'm thinking that the acetone does something to the binding sites rendering them unable to take either the Ab. Or maybe they can take the Ab but the chromagen step is compromised by the acetone. Either way it wouldn't work, we stopped doing it and our staining was fine. I suspect this problem would happen with any Ab and probably any chromagen. Any chemistry gurus out there could weigh in, but I don't think you'll be able to stain those biopsies. Hopefully you've got extras cut that are copies of the H and E? Maybe not as you've been asked to run IHC on them. I know this does not solve the problem, but maybe it explains why you're having it. Good luck, Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JMyers1@aol.com Sent: Monday, June 23, 2008 8:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC staining of previously H&E-stained prostate cores I'm trying to stain previously H&E-stained prostate core biopsies for HMW-CK and P504S, and I've been obtaining less than ideal results. I suspect that this might be caused by inadequate removal of mounting media (once the tape-coverslip is removed with acetone) or over/under- retrieval, but I could really use some advice from someone who has already worked through a situation like this. **************Gas prices getting you down? Search AOL Autos for fuel-efficient used cars. (http://autos.aol.com/used?ncid=aolaut00050000000007) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From suriana <@t> spdscientific.com.sg Mon Jun 23 20:38:45 2008 From: suriana <@t> spdscientific.com.sg (Suriana Bte Abdul Latiff) Date: Mon Jun 23 20:39:03 2008 Subject: [Histonet] Tissue-Tek Xpress x50 In-Reply-To: <1CE1847DFEA0A647B1CCDE4108EA60A7F23BEF@LTA3VS011.ees.hhs.gov> Message-ID: <2EF74098586A7B44B45162438E0F3CD27C55BA@Lithium.LABGISTICS.COM> Hi Everyone, I suggest you should try Pathos, microwave processor from Milestone. It is much superior in processing quality which I have tested it myself. It has a delay start feature allowing an overnight processing using microwave technology so no worries of overheating the samples. Trust me it is so wonderful. You may want to visit: http://www.milestonemed.com/microwave.php Sue -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Tuesday, June 24, 2008 1:11 AM To: Rene J Buesa; kimtournear@yahoo.com; Histonet Subject: RE: [Histonet] Tissue-Tek Xpress x50 The last retort is a heated, empty "bath"........you should NOT leave the tissue there for very long at all. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, June 23, 2008 1:07 PM To: kimtournear@yahoo.com; Histonet Subject: Re: [Histonet] Tissue-Tek Xpress x50 Both Xpress models have been engineered to process in a continuous flow of cassettes not for overnight (delayed) processing. Perhaps you could leave the cassettes in the last retort, but I am not sure about that nor it is recommended, and it would defeat the design of the instrument. Ren? J. Kim Tournear wrote: Hi, Is anyone using the Tissue-Tek Xpress x50 out there? Do you like it? Can you do traditional overnight processing or is it only for rapid processing? Any feed back would be helpful... Thanks, ~Kim ~ Tucson Medical Center Tucson, AZ ~Don't let your life end before it begins~ OU Rocks!!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Tue Jun 24 09:46:53 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Tue Jun 24 09:50:30 2008 Subject: [Histonet] Tissue-Tek Xpress x50 References: <2EF74098586A7B44B45162438E0F3CD27C55BA@Lithium.LABGISTICS.COM> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A73DF65B@LTA3VS011.ees.hhs.gov> I will say the Pathos sounds great but I wonder why one would want to pay for the microwave technology and not take full use of it, meaning you don't overnight process. But I do want to reiterate that the Xpress provides exceptional quality. I cannot compare it to the Pathos as it was not available when I purchased the Xpress but the Xpress is easy to use, exceptionally easy to maintain and provides wonderful results. ________________________________ From: Suriana Bte Abdul Latiff [mailto:suriana@spdscientific.com.sg] Sent: Mon 6/23/2008 9:38 PM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Rene J Buesa; kimtournear@yahoo.com; Histonet Subject: RE: [Histonet] Tissue-Tek Xpress x50 Hi Everyone, I suggest you should try Pathos, microwave processor from Milestone. It is much superior in processing quality which I have tested it myself. It has a delay start feature allowing an overnight processing using microwave technology so no worries of overheating the samples. Trust me it is so wonderful. You may want to visit: http://www.milestonemed.com/microwave.php Sue -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Tuesday, June 24, 2008 1:11 AM To: Rene J Buesa; kimtournear@yahoo.com; Histonet Subject: RE: [Histonet] Tissue-Tek Xpress x50 The last retort is a heated, empty "bath"........you should NOT leave the tissue there for very long at all. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, June 23, 2008 1:07 PM To: kimtournear@yahoo.com; Histonet Subject: Re: [Histonet] Tissue-Tek Xpress x50 Both Xpress models have been engineered to process in a continuous flow of cassettes not for overnight (delayed) processing. Perhaps you could leave the cassettes in the last retort, but I am not sure about that nor it is recommended, and it would defeat the design of the instrument. Ren? J. Kim Tournear wrote: Hi, Is anyone using the Tissue-Tek Xpress x50 out there? Do you like it? Can you do traditional overnight processing or is it only for rapid processing? Any feed back would be helpful... Thanks, ~Kim ~ Tucson Medical Center Tucson, AZ ~Don't let your life end before it begins~ OU Rocks!!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Jun 24 09:59:56 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jun 24 10:00:00 2008 Subject: [Histonet] Tissue-Tek Xpress x50 In-Reply-To: <1CE1847DFEA0A647B1CCDE4108EA60A73DF65B@LTA3VS011.ees.hhs.gov> Message-ID: <156198.61718.qm@web65709.mail.ac4.yahoo.com> Jeanine: All you have pointed out about the Xpress is true BUT you have to have THIN (1.5 mm) tissue slices to obtain optimal results and breast specimens have to be previously fixed (cannot be placed into the Xpress immediately after resection). The Pathos, on the other hand, can handle specimens up to 5 mm thick which sometimes happens when somebody doing grossing is not paying attention to detail. Besides, Xpress can handle less cassettes / basket full, another aspect not limited by the Pathos. Which means that each instruments has its own strong and weak points and all boils down to personal preferences of the user, and needs of the institution. Ren? J. "Bartlett, Jeanine (CDC/CCID/NCZVED)" wrote: From vanikodela <@t> hotmail.com Tue Jun 24 10:41:12 2008 From: vanikodela <@t> hotmail.com (vani) Date: Tue Jun 24 10:41:19 2008 Subject: [Histonet] Solvent Recycler Message-ID: We have a Xylene/Alcohol/Acetone recycler from CBG Biotech. The recycler is about 2 years old and is in a perfect condition. We have hardly used it. We bought it thinking that we will use it but decided to close that project. Anybody who is interested: please call me at 617-638-5244. Kodela Vani Research scientist MDP, LLC. Boston, MA 02118 From dellav <@t> musc.edu Tue Jun 24 11:59:43 2008 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Tue Jun 24 11:57:46 2008 Subject: [Histonet] Tissue-Tek Xpress x50 In-Reply-To: <2EF74098586A7B44B45162438E0F3CD27C55BA@Lithium.LABGISTICS.COM> References: <1CE1847DFEA0A647B1CCDE4108EA60A7F23BEF@LTA3VS011.ees.hhs.gov> <2EF74098586A7B44B45162438E0F3CD27C55BA@Lithium.LABGISTICS.COM> Message-ID: Sue, what is your affiliation with Milestone? Isn't your company a partner with Milestone? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Suriana Bte Abdul Latiff Sent: Monday, June 23, 2008 9:39 PM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Rene J Buesa; kimtournear@yahoo.com; Histonet Subject: RE: [Histonet] Tissue-Tek Xpress x50 Hi Everyone, I suggest you should try Pathos, microwave processor from Milestone. It is much superior in processing quality which I have tested it myself. It has a delay start feature allowing an overnight processing using microwave technology so no worries of overheating the samples. Trust me it is so wonderful. You may want to visit: http://www.milestonemed.com/microwave.php Sue -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Tuesday, June 24, 2008 1:11 AM To: Rene J Buesa; kimtournear@yahoo.com; Histonet Subject: RE: [Histonet] Tissue-Tek Xpress x50 The last retort is a heated, empty "bath"........you should NOT leave the tissue there for very long at all. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, June 23, 2008 1:07 PM To: kimtournear@yahoo.com; Histonet Subject: Re: [Histonet] Tissue-Tek Xpress x50 Both Xpress models have been engineered to process in a continuous flow of cassettes not for overnight (delayed) processing. Perhaps you could leave the cassettes in the last retort, but I am not sure about that nor it is recommended, and it would defeat the design of the instrument. Ren? J. Kim Tournear wrote: Hi, Is anyone using the Tissue-Tek Xpress x50 out there? Do you like it? Can you do traditional overnight processing or is it only for rapid processing? Any feed back would be helpful... Thanks, ~Kim ~ Tucson Medical Center Tucson, AZ ~Don't let your life end before it begins~ OU Rocks!!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmiller <@t> physlab.com Tue Jun 24 12:00:48 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Tue Jun 24 11:59:27 2008 Subject: [Histonet] hematoxilyn Message-ID: <000301c8d61b$d9ce82f0$3d02a8c0@plab.local> Anyone know what the current status of the hematoxilyn shortage is?? Is there even a shortage?? The supplier for my Histology hematoxilyn is back ordered to September and has given me a synthetic replacement to try. My cytology hematoxilyn supplier says they haven't heard of any shortage. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From Janet.Bonner <@t> FLHOSP.ORG Tue Jun 24 12:38:27 2008 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Tue Jun 24 12:40:14 2008 Subject: [Histonet] hematoxilyn References: <000301c8d61b$d9ce82f0$3d02a8c0@plab.local> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F26D7@fhosxchmb006.ADVENTISTCORP.NET> Our Richard-Allen Rep told us that the Hematoxylin 7231, 7221, and 7211 are not included in the Phoenix Blue substitution and that they are still available. Janet L. Bonner, HTL (ASCP) Pathology Laboratory Florida Hospital Winter Park janet.bonner@FLHOSP.org 407-646-7559 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cheri Miller Sent: Tue 6/24/2008 1:00 PM To: Histonet@lists.utsouthwestern.edu Cc: histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] hematoxilyn Anyone know what the current status of the hematoxilyn shortage is?? Is there even a shortage?? The supplier for my Histology hematoxilyn is back ordered to September and has given me a synthetic replacement to try. My cytology hematoxilyn supplier says they haven't heard of any shortage. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From RSRICHMOND <@t> aol.com Tue Jun 24 12:42:55 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Tue Jun 24 12:43:02 2008 Subject: [Histonet] Re: MarginMap for breast biopsy orientation Message-ID: Working in a small hospital in Georgia this week - just found out about a very useful new breast biopsy product (I have no commercial interest in it). MarginMap "charms" are little (5 and 10 mm sizes are available) metal markers used to mark the orientation of breast specimens - stamped through the metal are the words cranial, caudal, medial, deep, lateral, and skin. The surgeon attaches the charm - they look vaguely like the metal tokens that are hung on an old fashioned charm bracelet - to the specimen with a suture. The resulting tag is easily read on a specimen radiogram and by the pathologist grossing the specimen. Much simpler for the surgeon than tying all those short sutures and long sutures while trying to get a half-literate assistant to write it all down correctly. MarginMap is made by the Beekley Corporation in Bristol, Connecticut (beekley.com), which also makes the more familiar breast biopsy products AccuGrid and CoreTainer. These products are well illustrated on their Web site. Bob Richmond Samurai Pathologist Knoxville TN From HornHV <@t> archildrens.org Tue Jun 24 12:51:32 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Jun 24 12:51:38 2008 Subject: [Histonet] hematoxilyn In-Reply-To: <000301c8d61b$d9ce82f0$3d02a8c0@plab.local> References: <000301c8d61b$d9ce82f0$3d02a8c0@plab.local> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82CEC@EMAIL.archildrens.org> I've had hematoxylin ordered since April and it is still on back order... Then I tried another source and Fisher told me they sent their last 2 bottles to me..Don't know if that is true but there does indeed seem to be a shortage. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Tuesday, June 24, 2008 12:01 PM To: Histonet@lists.utsouthwestern.edu Cc: histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] hematoxilyn Anyone know what the current status of the hematoxilyn shortage is?? Is there even a shortage?? The supplier for my Histology hematoxilyn is back ordered to September and has given me a synthetic replacement to try. My cytology hematoxilyn supplier says they haven't heard of any shortage. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From jwatson <@t> gnf.org Tue Jun 24 12:56:21 2008 From: jwatson <@t> gnf.org (James Watson) Date: Tue Jun 24 12:56:30 2008 Subject: [Histonet] hematoxylin In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82CEC@EMAIL.archildrens.org> References: <000301c8d61b$d9ce82f0$3d02a8c0@plab.local> <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82CEC@EMAIL.archildrens.org> Message-ID: At the recent Biological staining Commission meeting in San Diego one of the 2 manufactures of Hematoxylin powder that supplies all the hematoxylin to the Vendors stated that due to a couple of reasons the supply was depleted and by June or July they would be filling the back orders to the hematoxylin vendors and that by this fall they would be caught up. They have taken measures to ensure that their supplies would not be depleted again. Some one from the BSC was supposed to post to the Histonet an explanation of this. James Watson HT ASCP Facilities Manager of Histology GNF Genomics Institute of the Novartis Research Foundation Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Tuesday, June 24, 2008 10:52 AM To: Cheri Miller; Histonet@lists.utsouthwestern.edu Cc: histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] hematoxilyn I've had hematoxylin ordered since April and it is still on back order... Then I tried another source and Fisher told me they sent their last 2 bottles to me..Don't know if that is true but there does indeed seem to be a shortage. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Tuesday, June 24, 2008 12:01 PM To: Histonet@lists.utsouthwestern.edu Cc: histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] hematoxilyn Anyone know what the current status of the hematoxilyn shortage is?? Is there even a shortage?? The supplier for my Histology hematoxilyn is back ordered to September and has given me a synthetic replacement to try. My cytology hematoxilyn supplier says they haven't heard of any shortage. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ****************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kimtournear <@t> yahoo.com Tue Jun 24 13:26:44 2008 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Tue Jun 24 13:26:51 2008 Subject: [Histonet] RE: Tissue-Tek Xpress x50 processor Message-ID: <763981.89761.qm@web50304.mail.re2.yahoo.com> Hi Everyone, Thanks for the input on this. Is there anyone out there running 100% of their work on these machines? How about the antibodies? Are there any antibodies that won't work? I heard the Her2 doesn't do well. Is validation difficult? I need more input...LOL...thanks everyone ? ~Kim ~ Tucson Medical Center Tucson, AZ ? ~Don't?let your life end before it begins~ ? OU Rocks!!!! From plucas <@t> biopath.org Tue Jun 24 13:32:51 2008 From: plucas <@t> biopath.org (Paula Lucas) Date: Tue Jun 24 13:32:54 2008 Subject: [Histonet] Per Diem Histotech needed in South Orange County Message-ID: <20080624183251.1CC3756F5@alexander.cnchost.com> Private lab in Fountain Valley, California is in need of a per diem histotech that can embed and cut surgical tissue cases. We are looking for someone who can fill in for vacation days taken during the year. We work Tuesday through Saturday, from 4 am to 1 pm. We can be flexible, meaning, we will take someone who can work 1 day or multiple days out of the work week, and/or from 1 to 6 hours a day. If you work a grave yard shift, you can come in after your shift. Or, if you work the mid-shift, you can come in before your shift starts. Thanks, Paula Lucas Bio-Path Medical Group 714-433-1330 From llewllew <@t> shaw.ca Tue Jun 24 13:56:42 2008 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Tue Jun 24 13:56:48 2008 Subject: [Histonet] hematoxilyn References: <000301c8d61b$d9ce82f0$3d02a8c0@plab.local> Message-ID: <002601c8d62c$0a97ba40$0a514246@yourlk4rlmsu> http://www.abbeycolor.com/index.php The url above is to a wholesale dye supply company that says it can supply bulk certified hematoxylin immediately (up to 1000 pounds). If so, there is no shortage. Bryan Llewellyn ----- Original Message ----- From: "Cheri Miller" To: Cc: Sent: Tuesday, June 24, 2008 10:00 AM Subject: [Histonet] hematoxilyn > Anyone know what the current status of the hematoxilyn shortage is?? Is > there even a shortage?? The supplier for my Histology hematoxilyn is back > ordered to September and has given me a synthetic replacement to try. My > cytology hematoxilyn supplier says they haven't heard of any shortage. > > > > Cheryl Miller HT (ASCP) > > Histology Supervisor > > Physicians Laboratory,P.C. > > Omaha, Ne. > > 402 738 5052 > > > > > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. > If you are not the addressee intended / indicated or agent responsible for > delivering it to the addressee, you are hereby notified that you are in > possession of confidential and privileged information. Any dissemination, > distribution, or copying of this e-mail is strictly prohibited. If you > have received this message in error, please notify the sender immediately > and delete this email from your system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tanisha.mcknight <@t> covance.com Tue Jun 24 14:42:22 2008 From: tanisha.mcknight <@t> covance.com (McKnight, Tanisha) Date: Tue Jun 24 14:42:39 2008 Subject: [Histonet] How to validate staining protocols? Message-ID: <816E3C72F855F14985FC31D7C963AE6F07D039FC@indexch03.ent.covance.com> Hello: Would someone mind sharing with me how to validate staining protocols in the histology laboratory? I need to validate an H&E stain, Giemsa and Trichrome. How many tissue types are required? Are there any CAP, FDA regulations regarding how this is done? Any help would be appreciated. Thanks, Tanisha ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From jwatson <@t> gnf.org Tue Jun 24 14:45:59 2008 From: jwatson <@t> gnf.org (James Watson) Date: Tue Jun 24 14:46:05 2008 Subject: [Histonet] hematoxylin In-Reply-To: References: <000301c8d61b$d9ce82f0$3d02a8c0@plab.local> <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82CEC@EMAIL.archildrens.org> Message-ID: Reason for depletion was stated as (if my memory serves me correctly): 1) The vendors depleted their stocks of powder to use up their older supplies then they all restocked their supplies from the hematoxylin powder suppliers at once. The company at the meeting is going to increase it's backup stock so this can be avoided. 2) The company that harvest the liquid hematoxylin from the logwood trees switched their harvesting to a makeup product that was more profitable, the company that was at the meeting has made a multi-year contract with this company so this should not happen again. Note I used the word depletion, not shortage. I personally have not had a problem getting the powder since I make up all my hematoxylin solutions. -----Original Message----- From: Dana Settembre [mailto:settembr@umdnj.edu] Sent: Tuesday, June 24, 2008 11:22 AM To: James Watson Subject: RE: [Histonet] hematoxylin What were the reasons for the depletion? Did they say? Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA -----Original Message----- From: James Watson Sent: Tuesday, June 24, 2008 10:56 AM To: 'Horn, Hazel V'; Cheri Miller; Histonet@lists.utsouthwestern.edu Cc: histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] hematoxylin At the recent Biological staining Commission meeting in San Diego one of the 2 manufactures of Hematoxylin powder that supplies all the hematoxylin to the Vendors stated that due to a couple of reasons the supply was depleted and by June or July they would be filling the back orders to the hematoxylin vendors and that by this fall they would be caught up. They have taken measures to ensure that their supplies would not be depleted again. Some one from the BSC was supposed to post to the Histonet an explanation of this. James Watson HT ASCP Facilities Manager of Histology GNF Genomics Institute of the Novartis Research Foundation Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Tuesday, June 24, 2008 10:52 AM To: Cheri Miller; Histonet@lists.utsouthwestern.edu Cc: histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] hematoxilyn I've had hematoxylin ordered since April and it is still on back order... Then I tried another source and Fisher told me they sent their last 2 bottles to me..Don't know if that is true but there does indeed seem to be a shortage. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Tuesday, June 24, 2008 12:01 PM To: Histonet@lists.utsouthwestern.edu Cc: histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] hematoxilyn Anyone know what the current status of the hematoxilyn shortage is?? Is there even a shortage?? The supplier for my Histology hematoxilyn is back ordered to September and has given me a synthetic replacement to try. My cytology hematoxilyn supplier says they haven't heard of any shortage. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ************************************************************************ ****************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From int09018 <@t> alphahunt.com Tue Jun 24 15:03:19 2008 From: int09018 <@t> alphahunt.com (special address) Date: Tue Jun 24 15:03:27 2008 Subject: [Histonet] IHC question Message-ID: <000601c8d635$593dc410$6500a8c0@LHBLION> Name: Xiangming Li E-mail: xli@vet.ksu.edu Phone: 785-532-4865 message: Hi, I m looking for bone specific fluorescence dye for bony otic capsule study. Can you give me some suggestion? besides, what should be noticed when doing fixation and cryosection of bone. Thank you. Respond to : xli@vet.ksu.edu From Kimberly.Marshall <@t> ahss.org Tue Jun 24 16:39:18 2008 From: Kimberly.Marshall <@t> ahss.org (Marshall, Kimberly) Date: Tue Jun 24 16:39:32 2008 Subject: [Histonet] Hematoxylin shortage Message-ID: Hello all Just wanting to ask around to other techs and find out how everyone else is handling the Hematoxylin shortage. My supplier put "Phoenix Blue Nuclear stain" in its place. Has anyone else used this and how was the nuclear detail. Thanks Kimberly Marshall H.T. (ASCP) ============================================================================== The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ============================================================================== From paw555 <@t> yahoo.com Tue Jun 24 16:40:40 2008 From: paw555 <@t> yahoo.com (pam plumlee) Date: Tue Jun 24 16:40:43 2008 Subject: [Histonet] Tips for cutting frozen mouse and rat eyes Message-ID: <44451.64972.qm@web31914.mail.mud.yahoo.com> Hi all: I have a project that requires me to cut frozen sections and stain H&E on mouse eyes. I have some practice eyes I am having issues with and was hoping I could talk to someone off line (or on) about helpful hints, tried and true methods, ect. Thanks so much, Pam Plumlee H.T. Pfizer La Jolla pam.plumlee@pfizer.com From suriana <@t> spdscientific.com.sg Tue Jun 24 19:46:32 2008 From: suriana <@t> spdscientific.com.sg (Suriana Bte Abdul Latiff) Date: Tue Jun 24 19:47:06 2008 Subject: [Histonet] Tissue-Tek Xpress x50 In-Reply-To: Message-ID: <2EF74098586A7B44B45162438E0F3CD27C5878@Lithium.LABGISTICS.COM> Hi Della, Yes, my current company is handling / selling histopathology products. I am now working on a project with all pathologists who are evaluating both Xpess and Pathos. Regardless of my company's partnership with Milestone, I used to be a histo technologist too for 4 years and that is the reason why I have personally chosen Pathos over Xpress. The software is far more flexible and user's not being "lock" with any special reagents. In order word, its an open system so no worries .. Everyone should really play with Pathos to bring histology to the next level. Sue Thank you and best regards, Suriana (Ms) | Senior Product Specialist | SPD Scientific Pte Ltd (Co. Reg. No. 199401685W) DID: +65 6303 9821 | Fax: +65 6775 0995 | Mobile: +65 9476 0203 | Email: suriana@spdscientifc.com.sg 192 Pandan Loop, #06-20 Pantech Industrial Complex, Singapore 128381 | www.biomediagroup.com If you have any feedback or comment on our services or products, please email us at feedback@biomediagroup.com A member of Biomedia Group -----Original Message----- From: Della Speranza, Vinnie [mailto:dellav@musc.edu] Sent: Wednesday, June 25, 2008 1:00 AM To: Suriana Bte Abdul Latiff; Bartlett, Jeanine (CDC/CCID/NCZVED); Rene J Buesa; kimtournear@yahoo.com; Histonet Subject: RE: [Histonet] Tissue-Tek Xpress x50 Sue, what is your affiliation with Milestone? Isn't your company a partner with Milestone? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Suriana Bte Abdul Latiff Sent: Monday, June 23, 2008 9:39 PM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Rene J Buesa; kimtournear@yahoo.com; Histonet Subject: RE: [Histonet] Tissue-Tek Xpress x50 Hi Everyone, I suggest you should try Pathos, microwave processor from Milestone. It is much superior in processing quality which I have tested it myself. It has a delay start feature allowing an overnight processing using microwave technology so no worries of overheating the samples. Trust me it is so wonderful. You may want to visit: http://www.milestonemed.com/microwave.php Sue -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Tuesday, June 24, 2008 1:11 AM To: Rene J Buesa; kimtournear@yahoo.com; Histonet Subject: RE: [Histonet] Tissue-Tek Xpress x50 The last retort is a heated, empty "bath"........you should NOT leave the tissue there for very long at all. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, June 23, 2008 1:07 PM To: kimtournear@yahoo.com; Histonet Subject: Re: [Histonet] Tissue-Tek Xpress x50 Both Xpress models have been engineered to process in a continuous flow of cassettes not for overnight (delayed) processing. Perhaps you could leave the cassettes in the last retort, but I am not sure about that nor it is recommended, and it would defeat the design of the instrument. Ren? J. Kim Tournear wrote: Hi, Is anyone using the Tissue-Tek Xpress x50 out there? Do you like it? Can you do traditional overnight processing or is it only for rapid processing? Any feed back would be helpful... Thanks, ~Kim ~ Tucson Medical Center Tucson, AZ ~Don't let your life end before it begins~ OU Rocks!!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMahoney <@t> alegent.org Wed Jun 25 07:16:49 2008 From: JMahoney <@t> alegent.org (Mahoney,Janice A) Date: Wed Jun 25 07:17:02 2008 Subject: [Histonet] RE: Hematoxylin shortage In-Reply-To: References: Message-ID: <346E5878979BA54FB4B0BFD6AD93B9B9B03945346C@EXCHMBC1.ad.ah.local> We tried Phoenix Blue to replace the Harris Hematoxylin in our DRS stainer and for Cytology PAP and nyn-gyn staining. We had to increase the staining time by about a minute and differentiate an additional 30 sec for H&E's but the results were pretty good. We happy knowing we have an alternative if needed. Jan Mahoney Alegent Health Omaha, NE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall, Kimberly Sent: Tuesday, June 24, 2008 4:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hematoxylin shortage Hello all Just wanting to ask around to other techs and find out how everyone else is handling the Hematoxylin shortage. My supplier put "Phoenix Blue Nuclear stain" in its place. Has anyone else used this and how was the nuclear detail. Thanks Kimberly Marshall H.T. (ASCP) ============================================================================== The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. 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From oshel1pe <@t> cmich.edu Wed Jun 25 07:29:31 2008 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Wed Jun 25 07:30:50 2008 Subject: [Histonet] Tissue-Tek Xpress x50 In-Reply-To: <2EF74098586A7B44B45162438E0F3CD27C5878@Lithium.LABGISTICS.COM> References: <2EF74098586A7B44B45162438E0F3CD27C5878@Lithium.LABGISTICS.COM> Message-ID: OK, it's not Friday, but ... Is it just me, or is "Pathos" a *really* bad name for a product? Maybe marketing types need to be given a good dictionary when they're hired. Phil >Hi Della, > >Yes, my current company is handling / selling >histopathology products. I am now working on a >project with all pathologists who are evaluating >both Xpess and Pathos. > >Regardless of my company's partnership with >Milestone, I used to be a histo technologist too >for 4 years and that is the reason why I have >personally chosen Pathos over Xpress. The >software is far more flexible and user's not >being "lock" with any special reagents. In order >word, its an open system so no worries .. > >Everyone should really play with Pathos to bring histology to the next level. > >Sue > > > > > > > >Thank you and best regards, > >Suriana (Ms) | Senior Product Specialist | SPD >Scientific Pte Ltd (Co. Reg. No. 199401685W) > >DID: +65 6303 9821 | Fax: +65 6775 0995 | >Mobile: +65 9476 0203 | Email: >suriana@spdscientifc.com.sg > >192 Pandan Loop, #06-20 Pantech Industrial >Complex, Singapore 128381 | www.biomediagroup.com > >If you have any feedback or comment on our >services or products, please email us at >feedback@biomediagroup.com > >A member of Biomedia Group > > >-----Original Message----- >From: Della Speranza, Vinnie [mailto:dellav@musc.edu] >Sent: Wednesday, June 25, 2008 1:00 AM >To: Suriana Bte Abdul Latiff; Bartlett, Jeanine >(CDC/CCID/NCZVED); Rene J Buesa; >kimtournear@yahoo.com; Histonet >Subject: RE: [Histonet] Tissue-Tek Xpress x50 > >Sue, what is your affiliation with Milestone? >Isn't your company a partner with Milestone? > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] >On Behalf Of Suriana Bte Abdul Latiff >Sent: Monday, June 23, 2008 9:39 PM >To: Bartlett, Jeanine (CDC/CCID/NCZVED); Rene J >Buesa; kimtournear@yahoo.com; Histonet >Subject: RE: [Histonet] Tissue-Tek Xpress x50 > > >Hi Everyone, > >I suggest you should try Pathos, microwave >processor from Milestone. It is much superior in >processing quality which I have tested it >myself. It has a delay start feature allowing an >overnight processing using microwave technology >so no worries of overheating the samples. > >Trust me it is so wonderful. You may want to >visit: http://www.milestonemed.com/microwave.php > > >Sue > > > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] >On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) >Sent: Tuesday, June 24, 2008 1:11 AM >To: Rene J Buesa; kimtournear@yahoo.com; Histonet >Subject: RE: [Histonet] Tissue-Tek Xpress x50 > >The last retort is a heated, empty >"bath"........you should NOT leave the tissue >there for very long at all. > > >Jeanine Bartlett >Infectious Diseases Pathology Branch >(404) 639-3590 >jeanine.bartlett@cdc.hhs.gov > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] >On Behalf Of Rene J Buesa >Sent: Monday, June 23, 2008 1:07 PM >To: kimtournear@yahoo.com; Histonet >Subject: Re: [Histonet] Tissue-Tek Xpress x50 > >Both Xpress models have been engineered to >process in a continuous flow of cassettes not >for overnight (delayed) processing. Perhaps you >could leave the cassettes in the last retort, >but I am not sure about that nor it is >recommended, and it would defeat the design of >the instrument. > Ren? J. > >Kim Tournear wrote: > Hi, >Is anyone using the Tissue-Tek Xpress x50 out >there? Do you like it? Can you do traditional >overnight processing or is it only for rapid >processing? Any feed back would be helpful... >Thanks, >~Kim ~ >Tucson Medical Center >Tucson, AZ > >~Don't let your life end before it begins~ > >OU Rocks!!!! > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576 From Malcolm.McCallum <@t> tamut.edu Wed Jun 25 08:00:58 2008 From: Malcolm.McCallum <@t> tamut.edu (Malcolm McCallum) Date: Wed Jun 25 08:04:13 2008 Subject: [Histonet] need quote on rotary microtome References: <502440.73722.qm@web50310.mail.re2.yahoo.com><519433.99746.qm@web65714.mail.ac4.yahoo.com> <1CE1847DFEA0A647B1CCDE4108EA60A7F23BEF@LTA3VS011.ees.hhs.gov> Message-ID: Hi, any vendors out there that could send me a price quote on a rotary microtome that has no bells and whistles beyond the ability to use both disposable and regular blades! :) I need a price on a refurbished and a new one. Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-223-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html VISIT HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org A New Journal Published in Partnership with Partners in Amphibian and Reptile Conservation and the World Congress of Herpetology. Spring Teaching Schedule & Office Hours: Genetics: W 6:00 to 9:40pm Herpetology: TR 10:00-11:40am Histology: MW 1:00-2:40pm Seminar: T 2:30-3:30pm Office Hours: M: 3:30-5:00pm T: 11:40-1:00pm; 3:30-5:00pm W: 4:00-6:00pm "We live in a time when lemonade is made with artificial flavoring, and furnisher polish is made with fresh lemons." -Alfred E. Neuman -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Mon 6/23/2008 12:10 PM To: Rene J Buesa; kimtournear@yahoo.com; Histonet Subject: RE: [Histonet] Tissue-Tek Xpress x50 The last retort is a heated, empty "bath"........you should NOT leave the tissue there for very long at all. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, June 23, 2008 1:07 PM To: kimtournear@yahoo.com; Histonet Subject: Re: [Histonet] Tissue-Tek Xpress x50 Both Xpress models have been engineered to process in a continuous flow of cassettes not for overnight (delayed) processing. Perhaps you could leave the cassettes in the last retort, but I am not sure about that nor it is recommended, and it would defeat the design of the instrument. Ren? J. Kim Tournear wrote: Hi, Is anyone using the Tissue-Tek Xpress x50 out there? Do you like it? Can you do traditional overnight processing or is it only for rapid processing? Any feed back would be helpful... Thanks, ~Kim ~ Tucson Medical Center Tucson, AZ ~Don't let your life end before it begins~ OU Rocks!!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From froyer <@t> bitstream.net Wed Jun 25 08:22:45 2008 From: froyer <@t> bitstream.net (Ford Royer) Date: Wed Jun 25 08:22:56 2008 Subject: OT RE: [Histonet] Tissue-Tek Xpress x50 In-Reply-To: <2EF74098586A7B44B45162438E0F3CD27C5878@Lithium.LABGISTICS.COM> References: <2EF74098586A7B44B45162438E0F3CD27C5878@Lithium.LABGISTICS.COM> Message-ID: <003a01c8d6c6$8e6c73c0$7701a80a@Ford> Hmmm... this sounds like a commercial to me. I?ve been flamed for a lot less on this list. (Maybe not as often as Joe the Toe, but flamed none the less). ;-) Editor?s Note: The emoticon [;-)] at the end of the above missal indicates humor. >From Webster?s Online Dictionary: PATHOS Pronunciation: \p?-th?s Function: noun Etymology: Greek, suffering, experience, emotion, from paschein (aor. pathein) to experience, suffer; perhaps akin to Lithuanian k?sti to suffer Date: 1591 1 : an element in experience or in artistic representation evoking pity or compassion 2 : an emotion of sympathetic pity Place in Tragedy ...the PATHOS, or suffering, of mankind. This distinction goes back to Aristotle, who, in the Rhetoric, distinguished between ethos, a man's natural bent, disposition, or moral character, and pathos, emotion displayed in a given situation. And the Latin rhetorician Quintilian, in the 1st century AD, noted that ethos is akin to comedy and pathos to tragedy. The distinction is important... IT?S HUMP DAY!... Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL:? 612-839-1046 Phone:? 763-542-8725 Fax:? 763-546-4830 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Suriana Bte Abdul Latiff Sent: Tuesday, June 24, 2008 7:47 PM To: Della Speranza, Vinnie; Bartlett, Jeanine (CDC/CCID/NCZVED); Rene J Buesa; kimtournear@yahoo.com; Histonet Subject: RE: [Histonet] Tissue-Tek Xpress x50 Hi Della, Yes, my current company is handling / selling histopathology products. I am now working on a project with all pathologists who are evaluating both Xpess and Pathos. Regardless of my company's partnership with Milestone, I used to be a histo technologist too for 4 years and that is the reason why I have personally chosen Pathos over Xpress. The software is far more flexible and user's not being "lock" with any special reagents. In order word, its an open system so no worries .. Everyone should really play with Pathos to bring histology to the next level. Sue Thank you and best regards, Suriana (Ms) | Senior Product Specialist | SPD Scientific Pte Ltd (Co. Reg. No. 199401685W) DID: +65 6303 9821 | Fax: +65 6775 0995 | Mobile: +65 9476 0203 | Email: suriana@spdscientifc.com.sg 192 Pandan Loop, #06-20 Pantech Industrial Complex, Singapore 128381 | www.biomediagroup.com If you have any feedback or comment on our services or products, please email us at feedback@biomediagroup.com A member of Biomedia Group -----Original Message----- From: Della Speranza, Vinnie [mailto:dellav@musc.edu] Sent: Wednesday, June 25, 2008 1:00 AM To: Suriana Bte Abdul Latiff; Bartlett, Jeanine (CDC/CCID/NCZVED); Rene J Buesa; kimtournear@yahoo.com; Histonet Subject: RE: [Histonet] Tissue-Tek Xpress x50 Sue, what is your affiliation with Milestone? Isn't your company a partner with Milestone? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Suriana Bte Abdul Latiff Sent: Monday, June 23, 2008 9:39 PM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Rene J Buesa; kimtournear@yahoo.com; Histonet Subject: RE: [Histonet] Tissue-Tek Xpress x50 Hi Everyone, I suggest you should try Pathos, microwave processor from Milestone. It is much superior in processing quality which I have tested it myself. It has a delay start feature allowing an overnight processing using microwave technology so no worries of overheating the samples. Trust me it is so wonderful. You may want to visit: http://www.milestonemed.com/microwave.php Sue -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Tuesday, June 24, 2008 1:11 AM To: Rene J Buesa; kimtournear@yahoo.com; Histonet Subject: RE: [Histonet] Tissue-Tek Xpress x50 The last retort is a heated, empty "bath"........you should NOT leave the tissue there for very long at all. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, June 23, 2008 1:07 PM To: kimtournear@yahoo.com; Histonet Subject: Re: [Histonet] Tissue-Tek Xpress x50 Both Xpress models have been engineered to process in a continuous flow of cassettes not for overnight (delayed) processing. Perhaps you could leave the cassettes in the last retort, but I am not sure about that nor it is recommended, and it would defeat the design of the instrument. Ren? J. Kim Tournear wrote: Hi, Is anyone using the Tissue-Tek Xpress x50 out there? Do you like it? Can you do traditional overnight processing or is it only for rapid processing? Any feed back would be helpful... Thanks, ~Kim ~ Tucson Medical Center Tucson, AZ ~Don't let your life end before it begins~ OU Rocks!!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Wed Jun 25 09:04:59 2008 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Wed Jun 25 09:03:04 2008 Subject: [Histonet] Tissue-Tek Xpress x50 In-Reply-To: <2EF74098586A7B44B45162438E0F3CD27C5878@Lithium.LABGISTICS.COM> References: <2EF74098586A7B44B45162438E0F3CD27C5878@Lithium.LABGISTICS.COM> Message-ID: While I respect your opinion Sue, when you are employed by a company that markets the very products that you are recommending, you have a responsibility to the Histonet members to inform them of this fact so that they can decide how they wish to interpret your remarks. You should also be aware that vendors are not permitted to sell or promote their products on Histonet and many have been flamed for attempting to do so. As a histotech you are free to express your professional opinions about the Pathos but I think it best that you be upfront about your affiliations with Milestone. I also want to be clear that my remarks are not intended to leave any impressions, pro or con, about either the Pathos or the Xpress instruments. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: Suriana Bte Abdul Latiff [mailto:suriana@spdscientific.com.sg] Sent: Tuesday, June 24, 2008 8:47 PM To: Della Speranza, Vinnie; Bartlett, Jeanine (CDC/CCID/NCZVED); Rene J Buesa; kimtournear@yahoo.com; Histonet Subject: RE: [Histonet] Tissue-Tek Xpress x50 Hi Della, Yes, my current company is handling / selling histopathology products. I am now working on a project with all pathologists who are evaluating both Xpess and Pathos. Regardless of my company's partnership with Milestone, I used to be a histo technologist too for 4 years and that is the reason why I have personally chosen Pathos over Xpress. The software is far more flexible and user's not being "lock" with any special reagents. In order word, its an open system so no worries .. Everyone should really play with Pathos to bring histology to the next level. Sue Thank you and best regards, Suriana (Ms) | Senior Product Specialist | SPD Scientific Pte Ltd (Co. Reg. No. 199401685W) DID: +65 6303 9821 | Fax: +65 6775 0995 | Mobile: +65 9476 0203 | Email: suriana@spdscientifc.com.sg 192 Pandan Loop, #06-20 Pantech Industrial Complex, Singapore 128381 | www.biomediagroup.com If you have any feedback or comment on our services or products, please email us at feedback@biomediagroup.com A member of Biomedia Group -----Original Message----- From: Della Speranza, Vinnie [mailto:dellav@musc.edu] Sent: Wednesday, June 25, 2008 1:00 AM To: Suriana Bte Abdul Latiff; Bartlett, Jeanine (CDC/CCID/NCZVED); Rene J Buesa; kimtournear@yahoo.com; Histonet Subject: RE: [Histonet] Tissue-Tek Xpress x50 Sue, what is your affiliation with Milestone? Isn't your company a partner with Milestone? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Suriana Bte Abdul Latiff Sent: Monday, June 23, 2008 9:39 PM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Rene J Buesa; kimtournear@yahoo.com; Histonet Subject: RE: [Histonet] Tissue-Tek Xpress x50 Hi Everyone, I suggest you should try Pathos, microwave processor from Milestone. It is much superior in processing quality which I have tested it myself. It has a delay start feature allowing an overnight processing using microwave technology so no worries of overheating the samples. Trust me it is so wonderful. You may want to visit: http://www.milestonemed.com/microwave.php Sue -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Tuesday, June 24, 2008 1:11 AM To: Rene J Buesa; kimtournear@yahoo.com; Histonet Subject: RE: [Histonet] Tissue-Tek Xpress x50 The last retort is a heated, empty "bath"........you should NOT leave the tissue there for very long at all. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, June 23, 2008 1:07 PM To: kimtournear@yahoo.com; Histonet Subject: Re: [Histonet] Tissue-Tek Xpress x50 Both Xpress models have been engineered to process in a continuous flow of cassettes not for overnight (delayed) processing. Perhaps you could leave the cassettes in the last retort, but I am not sure about that nor it is recommended, and it would defeat the design of the instrument. Ren? J. Kim Tournear wrote: Hi, Is anyone using the Tissue-Tek Xpress x50 out there? Do you like it? Can you do traditional overnight processing or is it only for rapid processing? Any feed back would be helpful... Thanks, ~Kim ~ Tucson Medical Center Tucson, AZ ~Don't let your life end before it begins~ OU Rocks!!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asachau <@t> titanmed.com Wed Jun 25 09:01:13 2008 From: asachau <@t> titanmed.com (April Sachau) Date: Wed Jun 25 09:05:00 2008 Subject: [Histonet] Histology job in Midwest In-Reply-To: Message-ID: <7E3ACD48BA6E26408F3188FBF08693F701579A15@titansbs1.corp.titanmed.com> Hello Histoland, I have yet a GREAT position available in the Midwest. Fast paced lab, very large volume. This position is Monday through Friday with no call. Compensation package includes car allowance ($150/week), housing allowance ($300/week - tax free), travel allowance (up to $800), tax free per diem ($210/week) and an hourly rate of $25 an hour! Please call me if you are interested or if you know of anyone that you think might be interested (Referral bonuses offered!). Email or call! Talk to you soon!!! April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com From dellav <@t> musc.edu Wed Jun 25 10:47:00 2008 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Wed Jun 25 10:45:29 2008 Subject: [Histonet] I need to clear the record In-Reply-To: References: <2EF74098586A7B44B45162438E0F3CD27C5878@Lithium.LABGISTICS.COM> Message-ID: It has been brought to my attention that my remarks to Sue regarding disclosure of her relationship with Milestone might be disingenuous because of my role as scientific editor of HistoLogic, a publication funded by Sakura, the manufacturer of the other microwave instrument that was under discussion. Because I made no attempt to promote either processor, I didn't think that my role as editor was relevant to the discussion however I now recognize that some may have thought that I responded to Sue as a result of my affiliation with HistoLogic. That never entered my mind. I just know that as a consumer of lab equipment like so many of you, I like to know when an individual may be promoting his or her products as opposed to someone offering a testimonial of success with a particular product. My interest in HistoLogic is to share what I hope is useful and educational information with those who might benefit and to help new authors get their work into print. At no time will you find me endorsing, selling or promoting anyone's products, including those marketed by Sakura, in my role as Scientific Editor for HistoLogic. I am grateful to Sakura for their generosity in making HistoLogic available to all of us and for allowing me complete freedom as editor to choose articles that will benefit the readers. As a colleague and fellow histotech, I do however reserve the right to take my editor's hat off periodically to offer an opinion about the best device or product if that would benefit someone else. I'll be careful in the future to make it clear which hat I am wearing when it is relevant to the post. I apologize to Sue and to you for my oversight in this case. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Wednesday, June 25, 2008 10:05 AM To: 'Suriana Bte Abdul Latiff'; Bartlett, Jeanine (CDC/CCID/NCZVED); Rene J Buesa; kimtournear@yahoo.com; Histonet Subject: RE: [Histonet] Tissue-Tek Xpress x50 While I respect your opinion Sue, when you are employed by a company that markets the very products that you are recommending, you have a responsibility to the Histonet members to inform them of this fact so that they can decide how they wish to interpret your remarks. You should also be aware that vendors are not permitted to sell or promote their products on Histonet and many have been flamed for attempting to do so. As a histotech you are free to express your professional opinions about the Pathos but I think it best that you be upfront about your affiliations with Milestone. I also want to be clear that my remarks are not intended to leave any impressions, pro or con, about either the Pathos or the Xpress instruments. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: Suriana Bte Abdul Latiff [mailto:suriana@spdscientific.com.sg] Sent: Tuesday, June 24, 2008 8:47 PM To: Della Speranza, Vinnie; Bartlett, Jeanine (CDC/CCID/NCZVED); Rene J Buesa; kimtournear@yahoo.com; Histonet Subject: RE: [Histonet] Tissue-Tek Xpress x50 Hi Della, Yes, my current company is handling / selling histopathology products. I am now working on a project with all pathologists who are evaluating both Xpess and Pathos. Regardless of my company's partnership with Milestone, I used to be a histo technologist too for 4 years and that is the reason why I have personally chosen Pathos over Xpress. The software is far more flexible and user's not being "lock" with any special reagents. In order word, its an open system so no worries .. Everyone should really play with Pathos to bring histology to the next level. Sue Thank you and best regards, Suriana (Ms) | Senior Product Specialist | SPD Scientific Pte Ltd (Co. Reg. No. 199401685W) DID: +65 6303 9821 | Fax: +65 6775 0995 | Mobile: +65 9476 0203 | Email: suriana@spdscientifc.com.sg 192 Pandan Loop, #06-20 Pantech Industrial Complex, Singapore 128381 | www.biomediagroup.com If you have any feedback or comment on our services or products, please email us at feedback@biomediagroup.com A member of Biomedia Group -----Original Message----- From: Della Speranza, Vinnie [mailto:dellav@musc.edu] Sent: Wednesday, June 25, 2008 1:00 AM To: Suriana Bte Abdul Latiff; Bartlett, Jeanine (CDC/CCID/NCZVED); Rene J Buesa; kimtournear@yahoo.com; Histonet Subject: RE: [Histonet] Tissue-Tek Xpress x50 Sue, what is your affiliation with Milestone? Isn't your company a partner with Milestone? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Suriana Bte Abdul Latiff Sent: Monday, June 23, 2008 9:39 PM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Rene J Buesa; kimtournear@yahoo.com; Histonet Subject: RE: [Histonet] Tissue-Tek Xpress x50 Hi Everyone, I suggest you should try Pathos, microwave processor from Milestone. It is much superior in processing quality which I have tested it myself. It has a delay start feature allowing an overnight processing using microwave technology so no worries of overheating the samples. Trust me it is so wonderful. You may want to visit: http://www.milestonemed.com/microwave.php Sue -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Tuesday, June 24, 2008 1:11 AM To: Rene J Buesa; kimtournear@yahoo.com; Histonet Subject: RE: [Histonet] Tissue-Tek Xpress x50 The last retort is a heated, empty "bath"........you should NOT leave the tissue there for very long at all. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, June 23, 2008 1:07 PM To: kimtournear@yahoo.com; Histonet Subject: Re: [Histonet] Tissue-Tek Xpress x50 Both Xpress models have been engineered to process in a continuous flow of cassettes not for overnight (delayed) processing. Perhaps you could leave the cassettes in the last retort, but I am not sure about that nor it is recommended, and it would defeat the design of the instrument. Ren? J. Kim Tournear wrote: Hi, Is anyone using the Tissue-Tek Xpress x50 out there? Do you like it? Can you do traditional overnight processing or is it only for rapid processing? Any feed back would be helpful... Thanks, ~Kim ~ Tucson Medical Center Tucson, AZ ~Don't let your life end before it begins~ OU Rocks!!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Carol.Fields <@t> Northside.com Wed Jun 25 11:04:28 2008 From: Carol.Fields <@t> Northside.com (Carol Fields) Date: Wed Jun 25 11:04:50 2008 Subject: [Histonet] Her2neu policy Message-ID: <038809F21D1F9040A1FEAD72A7E2C6B509724618@NSMXMS04.northside.local> Hi Netters, We are trying to put a policy in place for Her2neu on the fixation of breast specimens. We send the specimens out at this time and are not performing the Immunos. If any one would be so kind to share their policy it would be greatly appreciated. I am stumped on this after reading all the info on line. CAP question ANP.22998 ..... Does the laboratory have a documented procedure for ensuring appropriate length of fixation of specimens tested. Thank you in advance for any help. Carole Fields Northside Hospital Atlanta, GA CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. From Teri.Hallada <@t> midmichigan.org Wed Jun 25 11:11:38 2008 From: Teri.Hallada <@t> midmichigan.org (Teri.Hallada@midmichigan.org) Date: Wed Jun 25 11:11:47 2008 Subject: [Histonet] NSH Teleconference Message-ID: <8839B08E3ED7364E8CBBD53882C984D50994CCAA@MAILSRV01.midmichigan.net> I have not received my information on the teleconference today. Anyone out there have a number that I can call? Teresa Hallada BS, MT/CT (ASCP) Lead Cytotechnologist MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. From Teri.Hallada <@t> midmichigan.org Wed Jun 25 11:54:30 2008 From: Teri.Hallada <@t> midmichigan.org (Teri.Hallada@midmichigan.org) Date: Wed Jun 25 11:54:45 2008 Subject: [Histonet] Teleconference Message-ID: <8839B08E3ED7364E8CBBD53882C984D50994CCAD@MAILSRV01.midmichigan.net> Help!! I have everyone in the room to watch the teleconference and I have no information. Can someone please give me some assistance! Teresa Hallada BS, MT/CT (ASCP) Lead Cytotechnologist MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. From sbruce <@t> vetpathservicesinc.com Wed Jun 25 17:43:11 2008 From: sbruce <@t> vetpathservicesinc.com (Suzanne Bruce) Date: Wed Jun 25 17:45:25 2008 Subject: [Histonet] Vacuum Pumps Message-ID: <26DB1FDFBF9EE14AB3AACC873519A4A251507A@vpss1.VetPathServicesInc.local> Does anyone have any vacuum pumps that they aren't using or does anyone know of a site that sells used vacuum pumps? ________________________________________ Suzanne Bruce, R.V.T. Histologist / Necropsy Coordinator From jqb7 <@t> cdc.gov Wed Jun 25 17:49:27 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Jun 25 17:55:31 2008 Subject: [Histonet] Tissue-Tek Xpress x50 References: <2EF74098586A7B44B45162438E0F3CD27C5878@Lithium.LABGISTICS.COM> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A73DF65C@LTA3VS011.ees.hhs.gov> Almost as bad as Nemesis..... ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Philip Oshel Sent: Wed 6/25/2008 8:29 AM To: Histonet@Pathology.swmed.edu Subject: RE: [Histonet] Tissue-Tek Xpress x50 OK, it's not Friday, but ... Is it just me, or is "Pathos" a *really* bad name for a product? Maybe marketing types need to be given a good dictionary when they're hired. Phil >Hi Della, > >Yes, my current company is handling / selling >histopathology products. I am now working on a >project with all pathologists who are evaluating >both Xpess and Pathos. > >Regardless of my company's partnership with >Milestone, I used to be a histo technologist too >for 4 years and that is the reason why I have >personally chosen Pathos over Xpress. The >software is far more flexible and user's not >being "lock" with any special reagents. In order >word, its an open system so no worries .. > >Everyone should really play with Pathos to bring histology to the next level. > >Sue > > > > > > > >Thank you and best regards, > >Suriana (Ms) | Senior Product Specialist | SPD >Scientific Pte Ltd (Co. Reg. No. 199401685W) > >DID: +65 6303 9821 | Fax: +65 6775 0995 | >Mobile: +65 9476 0203 | Email: >suriana@spdscientifc.com.sg > >192 Pandan Loop, #06-20 Pantech Industrial >Complex, Singapore 128381 | www.biomediagroup.com > >If you have any feedback or comment on our >services or products, please email us at >feedback@biomediagroup.com > >A member of Biomedia Group > > >-----Original Message----- >From: Della Speranza, Vinnie [mailto:dellav@musc.edu] >Sent: Wednesday, June 25, 2008 1:00 AM >To: Suriana Bte Abdul Latiff; Bartlett, Jeanine >(CDC/CCID/NCZVED); Rene J Buesa; >kimtournear@yahoo.com; Histonet >Subject: RE: [Histonet] Tissue-Tek Xpress x50 > >Sue, what is your affiliation with Milestone? >Isn't your company a partner with Milestone? > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] >On Behalf Of Suriana Bte Abdul Latiff >Sent: Monday, June 23, 2008 9:39 PM >To: Bartlett, Jeanine (CDC/CCID/NCZVED); Rene J >Buesa; kimtournear@yahoo.com; Histonet >Subject: RE: [Histonet] Tissue-Tek Xpress x50 > > >Hi Everyone, > >I suggest you should try Pathos, microwave >processor from Milestone. It is much superior in >processing quality which I have tested it >myself. It has a delay start feature allowing an >overnight processing using microwave technology >so no worries of overheating the samples. > >Trust me it is so wonderful. You may want to >visit: http://www.milestonemed.com/microwave.php > > >Sue > > > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] >On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) >Sent: Tuesday, June 24, 2008 1:11 AM >To: Rene J Buesa; kimtournear@yahoo.com; Histonet >Subject: RE: [Histonet] Tissue-Tek Xpress x50 > >The last retort is a heated, empty >"bath"........you should NOT leave the tissue >there for very long at all. > > >Jeanine Bartlett >Infectious Diseases Pathology Branch >(404) 639-3590 >jeanine.bartlett@cdc.hhs.gov > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] >On Behalf Of Rene J Buesa >Sent: Monday, June 23, 2008 1:07 PM >To: kimtournear@yahoo.com; Histonet >Subject: Re: [Histonet] Tissue-Tek Xpress x50 > >Both Xpress models have been engineered to >process in a continuous flow of cassettes not >for overnight (delayed) processing. Perhaps you >could leave the cassettes in the last retort, >but I am not sure about that nor it is >recommended, and it would defeat the design of >the instrument. > Ren? J. > >Kim Tournear wrote: > Hi, >Is anyone using the Tissue-Tek Xpress x50 out >there? Do you like it? Can you do traditional >overnight processing or is it only for rapid >processing? Any feed back would be helpful... >Thanks, >~Kim ~ >Tucson Medical Center >Tucson, AZ > >~Don't let your life end before it begins~ > >OU Rocks!!!! > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Jun 25 18:02:35 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Jun 25 18:04:35 2008 Subject: [Histonet] Tissue-Tek Xpress x50 References: <156198.61718.qm@web65709.mail.ac4.yahoo.com> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A73DF65E@LTA3VS011.ees.hhs.gov> Of course each instrument has its pros and cons....I thought I was clear that I have not used the Pathos and was simply describing my use of the Xpress. As proper grossing technique is important with any type or brand of tissue processing, using the grossing tools Sakura sells would be useful for anyone grossing, period. We all know the "garbage in-garbage out" situation with improperly grossed tissues. Also, you are not limited to 1.5 mm......it can be increased if you adjust the processing time. Not to 5 mm, but it can be thicker. I am not sure I understand the statement you made regarding the Xpress being limited regarding less cassettes. But of course each lab should investigate whatever product suits their needs the best. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Tue 6/24/2008 10:59 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Suriana Bte Abdul Latiff; kimtournear@yahoo.com; Histonet Subject: RE: [Histonet] Tissue-Tek Xpress x50 Jeanine: All you have pointed out about the Xpress is true BUT you have to have THIN (1.5 mm) tissue slices to obtain optimal results and breast specimens have to be previously fixed (cannot be placed into the Xpress immediately after resection). The Pathos, on the other hand, can handle specimens up to 5 mm thick which sometimes happens when somebody doing grossing is not paying attention to detail. Besides, Xpress can handle less cassettes / basket full, another aspect not limited by the Pathos. Which means that each instruments has its own strong and weak points and all boils down to personal preferences of the user, and needs of the institution. Ren? J. "Bartlett, Jeanine (CDC/CCID/NCZVED)" wrote: _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From suriana <@t> spdscientific.com.sg Wed Jun 25 20:11:04 2008 From: suriana <@t> spdscientific.com.sg (Suriana Bte Abdul Latiff) Date: Wed Jun 25 20:11:18 2008 Subject: [Histonet] RE: I need to clear the record In-Reply-To: Message-ID: <2EF74098586A7B44B45162438E0F3CD27C5C53@Lithium.LABGISTICS.COM> Hi Della, No worries, there isn't any hard feelings involved :-) I truly agrees that Histonet should never be treated as a base in promoting products. Regards Sue ________________________________ From: Della Speranza, Vinnie [mailto:dellav@musc.edu] Sent: Wednesday, June 25, 2008 11:47 PM To: Della Speranza, Vinnie; Suriana Bte Abdul Latiff; Bartlett, Jeanine (CDC/CCID/NCZVED); Rene J Buesa; kimtournear@yahoo.com; Histonet Subject: I need to clear the record It has been brought to my attention that my remarks to Sue regarding disclosure of her relationship with Milestone might be disingenuous because of my role as scientific editor of HistoLogic, a publication funded by Sakura, the manufacturer of the other microwave instrument that was under discussion. Because I made no attempt to promote either processor, I didn't think that my role as editor was relevant to the discussion however I now recognize that some may have thought that I responded to Sue as a result of my affiliation with HistoLogic. That never entered my mind. I just know that as a consumer of lab equipment like so many of you, I like to know when an individual may be promoting his or her products as opposed to someone offering a testimonial of success with a particular product. My interest in HistoLogic is to share what I hope is useful and educational information with those who might benefit and to help new authors get their work into print. At no time will you find me endorsing, selling or promoting anyone's products, including those marketed by Sakura, in my role as Scientific Editor for HistoLogic. I am grateful to Sakura for their generosity in making HistoLogic available to all of us and for allowing me complete freedom as editor to choose articles that will benefit the readers. As a colleague and fellow histotech, I do however reserve the right to take my editor's hat off periodically to offer an opinion about the best device or product if that would benefit someone else. I'll be careful in the future to make it clear which hat I am wearing when it is relevant to the post. I apologize to Sue and to you for my oversight in this case. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Wednesday, June 25, 2008 10:05 AM To: 'Suriana Bte Abdul Latiff'; Bartlett, Jeanine (CDC/CCID/NCZVED); Rene J Buesa; kimtournear@yahoo.com; Histonet Subject: RE: [Histonet] Tissue-Tek Xpress x50 While I respect your opinion Sue, when you are employed by a company that markets the very products that you are recommending, you have a responsibility to the Histonet members to inform them of this fact so that they can decide how they wish to interpret your remarks. You should also be aware that vendors are not permitted to sell or promote their products on Histonet and many have been flamed for attempting to do so. As a histotech you are free to express your professional opinions about the Pathos but I think it best that you be upfront about your affiliations with Milestone. I also want to be clear that my remarks are not intended to leave any impressions, pro or con, about either the Pathos or the Xpress instruments. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: Suriana Bte Abdul Latiff [mailto:suriana@spdscientific.com.sg] Sent: Tuesday, June 24, 2008 8:47 PM To: Della Speranza, Vinnie; Bartlett, Jeanine (CDC/CCID/NCZVED); Rene J Buesa; kimtournear@yahoo.com; Histonet Subject: RE: [Histonet] Tissue-Tek Xpress x50 Hi Della, Yes, my current company is handling / selling histopathology products. I am now working on a project with all pathologists who are evaluating both Xpess and Pathos. Regardless of my company's partnership with Milestone, I used to be a histo technologist too for 4 years and that is the reason why I have personally chosen Pathos over Xpress. The software is far more flexible and user's not being "lock" with any special reagents. In order word, its an open system so no worries .. Everyone should really play with Pathos to bring histology to the next level. Sue Thank you and best regards, Suriana (Ms) | Senior Product Specialist | SPD Scientific Pte Ltd (Co. Reg. No. 199401685W) DID: +65 6303 9821 | Fax: +65 6775 0995 | Mobile: +65 9476 0203 | Email: suriana@spdscientifc.com.sg 192 Pandan Loop, #06-20 Pantech Industrial Complex, Singapore 128381 | www.biomediagroup.com If you have any feedback or comment on our services or products, please email us at feedback@biomediagroup.com A member of Biomedia Group -----Original Message----- From: Della Speranza, Vinnie [mailto:dellav@musc.edu] Sent: Wednesday, June 25, 2008 1:00 AM To: Suriana Bte Abdul Latiff; Bartlett, Jeanine (CDC/CCID/NCZVED); Rene J Buesa; kimtournear@yahoo.com; Histonet Subject: RE: [Histonet] Tissue-Tek Xpress x50 Sue, what is your affiliation with Milestone? Isn't your company a partner with Milestone? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Suriana Bte Abdul Latiff Sent: Monday, June 23, 2008 9:39 PM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Rene J Buesa; kimtournear@yahoo.com; Histonet Subject: RE: [Histonet] Tissue-Tek Xpress x50 Hi Everyone, I suggest you should try Pathos, microwave processor from Milestone. It is much superior in processing quality which I have tested it myself. It has a delay start feature allowing an overnight processing using microwave technology so no worries of overheating the samples. Trust me it is so wonderful. You may want to visit: http://www.milestonemed.com/microwave.php Sue -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Tuesday, June 24, 2008 1:11 AM To: Rene J Buesa; kimtournear@yahoo.com; Histonet Subject: RE: [Histonet] Tissue-Tek Xpress x50 The last retort is a heated, empty "bath"........you should NOT leave the tissue there for very long at all. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, June 23, 2008 1:07 PM To: kimtournear@yahoo.com; Histonet Subject: Re: [Histonet] Tissue-Tek Xpress x50 Both Xpress models have been engineered to process in a continuous flow of cassettes not for overnight (delayed) processing. Perhaps you could leave the cassettes in the last retort, but I am not sure about that nor it is recommended, and it would defeat the design of the instrument. Ren? J. Kim Tournear wrote: Hi, Is anyone using the Tissue-Tek Xpress x50 out there? Do you like it? Can you do traditional overnight processing or is it only for rapid processing? Any feed back would be helpful... Thanks, ~Kim ~ Tucson Medical Center Tucson, AZ ~Don't let your life end before it begins~ OU Rocks!!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From timscase <@t> gmail.com Thu Jun 26 05:56:50 2008 From: timscase <@t> gmail.com (Tim Scase) Date: Thu Jun 26 05:57:07 2008 Subject: [Histonet] Histology technician position at a veterinary histopathology laboratory in Bristol, UK Message-ID: <486375F2.5010905@gmail.com> Dear Histonetters, we are re-advertising an exciting opportunity for a senior histology technician in Bristol, UK to help us develop our new veterinary histopathology laboratory. The details are posted below. Best regards to you all, Tim. Bridge Pathology Ltd. is a recently registered company located in Bristol, that will specialize in the processing of biopsy specimens submitted by veterinary surgeons. We are searching for a dynamic and enthusiastic senior histology technician to work with the Director of Pathology to set up, manage and develop the histopathology and immunohistochemistry laboratory facilities and oversee the day-to-day operation of the histopathology and immunohistochemistry laboratories. Duties will include routine preparation of histological and immunohistochemical-stained sections of submitted biopsy tissues for diagnostic and research purposes. We will receive biopsy samples posted in from veterinary surgeons in practice. Tissues will be examined histologically and reports generated by veterinary pathologists, with an emphasis on providing the veterinary surgeon with as much specific, detailed and clinically useful information as possible. Where applicable, histochemical and immunohistochemical methods will be used to characterize the tissues further. In addition, samples received through the laboratory will be used for clinical research studies to further our understanding of animal disease and to develop prognostic and diagnostic assays to benefit veterinary patients. Results from these research studies will be submitted for publication in the scientific literature. We hope to establish Bridge Pathology Ltd. as the UK's premier diagnostic and research-focused veterinary histopathology laboratory. A successful applicant will have: 1) At least 3 years experience preparing tissue specimens for routine histopathological examination. 2) At least 2 years experience preparing histochemical and immunohistochemical stained sections. 3) Meticulous working habits with meticulous documentation of samples and processing thereof. 4) Experience developing laboratory standard operating procedures. 5) The ability to work well in a close-knit, friendly team. 6) The ability to work efficiently and to tight deadlines. 7) Good communication skills. 8) Basic computer skills. Application closing date: 9th July, 2008. Salary: ?25-28K + bonus For more information, please contact: Dr Tim Scase, Director of Pathology (07908 160354; tim@bridgepathology.com) From brett_connolly <@t> merck.com Thu Jun 26 08:27:58 2008 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Thu Jun 26 08:28:05 2008 Subject: [Histonet] IHC marker for M-phase cells Message-ID: <63EA0607835FBA4689CEA9EA8B48269201306BD2@usctmx1141.merck.com> I am looking for an antibody for labeling FFPE sections of mitotic cells that are only in M-phase. Found one article in J Histo Cyto using clone HTA28-phospho-Histone H3(Ser 28) that looked pretty good, but the Authors produced their own antibody. The commercial suppliers I have been able to locate do not indicate IHC as a suitable application for their products. Anyone had experience with this?? Or can you recommend another M-phase specific antibody? Many thanks, Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Research Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From oshel1pe <@t> cmich.edu Thu Jun 26 08:38:29 2008 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Thu Jun 26 08:39:14 2008 Subject: [Histonet] Vacuum Pumps In-Reply-To: <26DB1FDFBF9EE14AB3AACC873519A4A251507A@vpss1.VetPathServicesInc.local> References: <26DB1FDFBF9EE14AB3AACC873519A4A251507A@vpss1.VetPathServicesInc.local> Message-ID: Duniway Stockroom is the best source I know of. Also for parts and supplies, like pump oil. Phil >Does anyone have any vacuum pumps that they aren't using or does >anyone know of a site that sells used vacuum pumps? > >________________________________________ >Suzanne Bruce, R.V.T. >Histologist / Necropsy Coordinator >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576 From oshel1pe <@t> cmich.edu Thu Jun 26 08:46:06 2008 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Thu Jun 26 08:46:16 2008 Subject: [Histonet] Vacuum Pumps URL for Duniway In-Reply-To: References: <26DB1FDFBF9EE14AB3AACC873519A4A251507A@vpss1.VetPathServicesInc.local> Message-ID: Forgot this: http://www.duniway.com/ >Duniway Stockroom is the best source I know of. Also for parts and >supplies, like pump oil. >Phil > >>Does anyone have any vacuum pumps that they aren't using or does >>anyone know of a site that sells used vacuum pumps? >> >>________________________________________ >>Suzanne Bruce, R.V.T. >>Histologist / Necropsy Coordinator >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >-- >Philip Oshel >Microscopy Facility Supervisor >Biology Department >024C Brooks Hall >Central Michigan University >Mt. Pleasant, MI 48859 >(989) 774-3576 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Thu Jun 26 09:36:46 2008 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Thu Jun 26 09:39:24 2008 Subject: [Histonet] IHC marker for M-phase cells In-Reply-To: <63EA0607835FBA4689CEA9EA8B48269201306BD2@usctmx1141.merck.com> References: <63EA0607835FBA4689CEA9EA8B48269201306BD2@usctmx1141.merck.com> Message-ID: <1762567914-1214491158-cardhu_decombobulator_blackberry.rim.net-733031162-@bxe157.bisx.prod.on.blackberry> Phospho histone H3 from upstate biotechnology is fabulous on FFPE tissue. -----Original Message----- From: "Connolly, Brett M" Date: Thu, 26 Jun 2008 09:27:58 To:histonet@lists.utsouthwestern.edu, ihcrg@googlegroups.com Subject: [Histonet] IHC marker for M-phase cells I am looking for an antibody for labeling FFPE sections of mitotic cells that are only in M-phase. Found one article in J Histo Cyto using clone HTA28-phospho-Histone H3(Ser 28) that looked pretty good, but the Authors produced their own antibody. The commercial suppliers I have been able to locate do not indicate IHC as a suitable application for their products. Anyone had experience with this?? Or can you recommend another M-phase specific antibody? Many thanks, Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Research Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sjchtascp <@t> yahoo.com Thu Jun 26 10:40:13 2008 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Thu Jun 26 10:40:22 2008 Subject: [Histonet] Madison, WI Message-ID: <814713.51970.qm@web38207.mail.mud.yahoo.com> Looking for an HT?research position in Madison, WI.? If anyone has any possible leads, I would greatly appreciate your email. ? Thanks, ? Steve From jm.lapointe <@t> accellab.com Thu Jun 26 12:23:17 2008 From: jm.lapointe <@t> accellab.com (Jean-Martin Lapointe) Date: Thu Jun 26 12:27:40 2008 Subject: [Histonet] IHC marker for M-phase cells References: <200806261705.m5QH5VXW027944@gateway5.lastspam.com> Message-ID: Hi Brett, at my previous job we did IHC staining for phospho-Histone H3 (ser10) using the Cell Signaling rabbit monoclonal antibody CS9701 in FFPE tissue, and it worked beautifully. It was on human origin tissue, don't remember cross-reactivity in other species but Cell Signaling mentions it reacts with many others. Good luck Jean-Martin __________________________________ Jean-Martin Lapointe, DMV, MS, dACVP Vice-President, Pathologie AccelLAB Inc 1635 Lionel-Bertrand, Boisbriand Qu?bec, Canada J7H 1N8 tel:? 450-435-9482 ext.247 fax: 450-435-4795 jm.lapointe@accellab.com ? ? ________________________________________________________________ Message: 6 Date: Thu, 26 Jun 2008 09:27:58 -0400 From: "Connolly, Brett M" Subject: [Histonet] IHC marker for M-phase cells To: , Message-ID: <63EA0607835FBA4689CEA9EA8B48269201306BD2@usctmx1141.merck.com> Content-Type: text/plain; charset="us-ascii" I am looking for an antibody for labeling FFPE sections of mitotic cells that are only in M-phase. Found one article in J Histo Cyto using clone HTA28-phospho-Histone H3(Ser 28) that looked pretty good, but the Authors produced their own antibody. The commercial suppliers I have been able to locate do not indicate IHC as a suitable application for their products. Anyone had experience with this?? Or can you recommend another M-phase specific antibody? Many thanks, Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Research Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com ******************** From DixonM <@t> vetmed.ufl.edu Thu Jun 26 12:56:05 2008 From: DixonM <@t> vetmed.ufl.edu (MaryAnn Dixon) Date: Thu Jun 26 12:56:18 2008 Subject: [Histonet] HMB45 antibody Message-ID: Hi histonetters, Anyone out there successfully using a monoclonal HMB45 antibody on canine tissues? I think I've read a dozen papers all with different protocols and my eyes are about to pop out of my head! Currently I'm at a 1:50 dilution with a pretreatment in a citrate buffer (pressure cooker). I have my choice of a polymer based detection as well as a biotinylated detection. Any help would be appreciated. Thanks in advance. MaryAnn Dixon BS Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 392-2235 Ext. 4517 From John.Spair <@t> multicare.org Thu Jun 26 12:53:23 2008 From: John.Spair <@t> multicare.org (John Spair) Date: Thu Jun 26 13:43:45 2008 Subject: [Histonet] Formalin Recyling In-Reply-To: <647D0EF40TO16168-01@MMS_multicare.org> References: <647D0EF40TO16168-01@MMS_multicare.org> Message-ID: <61A9977919846C479389493BAE2517CA023760EC@MHSEXMBX1.multicare.org> Is anyone using the formalin recycling/filtration system sold by Creative Waste Solutions?? If you are , please email me at: john.spair@multicare.org if you don't mind, I have some questions. Thank you. "MMS " made the following annotations. ------------------------------------------------------------------------------ NOTICE: This e-mail and the attachments hereto, if any, may contain privileged and/or confidential information. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, you are hereby notified that any examination, distribution or copying of this e-mail and the attachments hereto, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by email or telephone and permanently delete this e-mail and the attachments hereto, if any, and destroy any printout thereof. MultiCare Health System, Tacoma, WA 98415 (253) 403-1000. ============================================================================== From shive003 <@t> umn.edu Thu Jun 26 15:10:29 2008 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu Jun 26 15:10:34 2008 Subject: [Histonet] HMB45 antibody References: Message-ID: I had little or no success with HMB45 on canine tissue. I use Melan-A exclusively; that works fine. Jan Shivers Senior Scientist Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu ----- Original Message ----- From: "MaryAnn Dixon" To: Sent: Thursday, June 26, 2008 12:56 PM Subject: [Histonet] HMB45 antibody Hi histonetters, Anyone out there successfully using a monoclonal HMB45 antibody on canine tissues? I think I've read a dozen papers all with different protocols and my eyes are about to pop out of my head! Currently I'm at a 1:50 dilution with a pretreatment in a citrate buffer (pressure cooker). I have my choice of a polymer based detection as well as a biotinylated detection. Any help would be appreciated. Thanks in advance. MaryAnn Dixon BS Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 392-2235 Ext. 4517 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gentras <@t> vetmed.auburn.edu Thu Jun 26 16:52:01 2008 From: gentras <@t> vetmed.auburn.edu (Atoska Gentry) Date: Thu Jun 26 16:52:05 2008 Subject: [Histonet] RE: whole mount cryosections Message-ID: <48640F81.7080301@vetmed.auburn.edu> hello, will someone with expertise in fresh frozen whole mount and/or coronal primate brain sections please contact me with pointers on requirements for this type set-up. Are attachments available for the Microm HM 505 E that will accommodate such samples? We normally section standard size sections but now one of our PI's is considering trying larger samples. Thanks, Atoska From petepath <@t> yahoo.com Thu Jun 26 17:52:50 2008 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Thu Jun 26 17:52:54 2008 Subject: [Histonet] whole mount cryosections Message-ID: <567183.62199.qm@web45114.mail.sp1.yahoo.com> Hi Atoska, ? Our 50 x 30 mm well bar set? with 55 x 35 mm chucks may give a bit more room to work. See item # 115 on this page: http://pathologyinnovations.com/Price%20List.htm ? Stephen ? From raj <@t> bluemarble.net Thu Jun 26 21:11:15 2008 From: raj <@t> bluemarble.net (Rebecca Johnson) Date: Thu Jun 26 21:11:16 2008 Subject: [Histonet] Prisma Stainer/coverslipper Message-ID: <013d01c86092$341481b0$7b48f9d8@CHURCH> Is anyone using the Prisma coverslipper in Cytology for the Imager? If so how are you doing it? Length of film and level. Thanks Becky From jmjohnson34 <@t> hotmail.com Fri Jun 27 08:32:59 2008 From: jmjohnson34 <@t> hotmail.com (Jennifer Johnson) Date: Fri Jun 27 08:33:04 2008 Subject: [Histonet] Vacuum pumps Message-ID: The James Watson Company sells new & used vacuum pumps. Their phone number is (770) 422-1154. I do not know if they have a web site. They are a direct source instead of having to buy from a medical supply company that will mark up your product. Jennifer Johnson, HTL (ASCP) P.S. I have an affiliation with this company. It is owned by my ex brother-in-law twice removed. _________________________________________________________________ The other season of giving begins 6/24/08. Check out the i?m Talkathon. http://www.imtalkathon.com?source=TXT_EML_WLH_SeasonOfGiving From brett_connolly <@t> merck.com Fri Jun 27 08:42:28 2008 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Fri Jun 27 08:42:37 2008 Subject: [Histonet] RE: IHC marker for M-phase cells Message-ID: <63EA0607835FBA4689CEA9EA8B48269201306E3A@usctmx1141.merck.com> Thanks to all who replied to my question. Seems like everyone uses anti-phosph-histone H3 (Ser10) as I have also used. However, the Ser10 antibody has also been shown to label interphase G2 cells (Goto et al., J Biol Chem 1999, 274: 25543-25549 and others) and I was really looking for an M-phase only marker. So if anyone has used the phospho-Ser28 histone H3 antibody I would be interested to hear your experiences. Brett > _____________________________________________ > From: Connolly, Brett M > Sent: Thursday, June 26, 2008 9:28 AM > To: histonet@lists.utsouthwestern.edu; ihcrg@googlegroups.com > Subject: IHC marker for M-phase cells > > I am looking for an antibody for labeling FFPE sections of mitotic > cells that are only in M-phase. Found one article in J Histo Cyto > using clone HTA28-phospho-Histone H3(Ser 28) that looked pretty good, > but the Authors produced their own antibody. > > The commercial suppliers I have been able to locate do not indicate > IHC as a suitable application for their products. > > Anyone had experience with this?? > > Or can you recommend another M-phase specific antibody? > > Many thanks, > Brett > > Brett M. Connolly, Ph.D. > Research Fellow, Imaging Research > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > PH 215-652-2501 fax. 215-993-6803 > e-mail. brett_connolly@merck.com > Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From pruegg <@t> ihctech.net Fri Jun 27 11:17:17 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Jun 27 11:17:24 2008 Subject: [Histonet] lab tek metal slide cabinets Message-ID: <005601c8d871$45f86570$6401a8c0@Patsyoffice> I have some brand new lab tek metal slide cabinets that are missing the drawer dividers, does anyone know where I can buy these? They turn the drawer into two so you can line up slides or blocks side by side, without these nothing fits in the drawer. Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From kolekams <@t> trinity-health.org Fri Jun 27 12:17:33 2008 From: kolekams <@t> trinity-health.org (Sarah Kolekamp) Date: Fri Jun 27 12:17:57 2008 Subject: [Histonet] Quality Assurance Measures Message-ID: <4864E86D0200006100015432@nodcgwcluster_gw4_server.no.trinity-health.org> Is anyone currently running a quality assurance program in their histology lab, if so what do you measure/how? We are tring to develop some standards and would like to have data to run analysis on...any ideas on what data to collect?? Thanks, Sarah Kolekamp Sarah Kolekamp HTL (ASCP) Lead Histotechnologist Saint Mary's Health Care Phone Number (616) 752-6155 Fax Number (616) 774-0628 >>> 06/27/08 1:02 PM >>> Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. IHC marker for M-phase cells (Jean-Martin Lapointe) 2. HMB45 antibody (MaryAnn Dixon) 3. Formalin Recyling (John Spair) 4. Re: HMB45 antibody (Jan Shivers) 5. RE: whole mount cryosections (Atoska Gentry) 6. whole mount cryosections (Stephen Peters M.D.) 7. Prisma Stainer/coverslipper (Rebecca Johnson) 8. Vacuum pumps (Jennifer Johnson) 9. RE: IHC marker for M-phase cells (Connolly, Brett M) 10. lab tek metal slide cabinets (Patsy Ruegg) ---------------------------------------------------------------------- Message: 1 Date: Thu, 26 Jun 2008 13:23:17 -0400 From: "Jean-Martin Lapointe" Subject: [Histonet] IHC marker for M-phase cells To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Brett, at my previous job we did IHC staining for phospho-Histone H3 (ser10) using the Cell Signaling rabbit monoclonal antibody CS9701 in FFPE tissue, and it worked beautifully. It was on human origin tissue, don't remember cross-reactivity in other species but Cell Signaling mentions it reacts with many others. Good luck Jean-Martin __________________________________ Jean-Martin Lapointe, DMV, MS, dACVP Vice-President, Pathologie AccelLAB Inc 1635 Lionel-Bertrand, Boisbriand Qu?bec, Canada J7H 1N8 tel:? 450-435-9482 ext.247 fax: 450-435-4795 jm.lapointe@accellab.com ? ? ________________________________________________________________ Message: 6 Date: Thu, 26 Jun 2008 09:27:58 -0400 From: "Connolly, Brett M" Subject: [Histonet] IHC marker for M-phase cells To: , Message-ID: <63EA0607835FBA4689CEA9EA8B48269201306BD2@usctmx1141.merck.com> Content-Type: text/plain; charset="us-ascii" I am looking for an antibody for labeling FFPE sections of mitotic cells that are only in M-phase. Found one article in J Histo Cyto using clone HTA28-phospho-Histone H3(Ser 28) that looked pretty good, but the Authors produced their own antibody. The commercial suppliers I have been able to locate do not indicate IHC as a suitable application for their products. Anyone had experience with this?? Or can you recommend another M-phase specific antibody? Many thanks, Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Research Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com ******************** ------------------------------ Message: 2 Date: Thu, 26 Jun 2008 13:56:05 -0400 From: "MaryAnn Dixon" Subject: [Histonet] HMB45 antibody To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi histonetters, Anyone out there successfully using a monoclonal HMB45 antibody on canine tissues? I think I've read a dozen papers all with different protocols and my eyes are about to pop out of my head! Currently I'm at a 1:50 dilution with a pretreatment in a citrate buffer (pressure cooker). I have my choice of a polymer based detection as well as a biotinylated detection. Any help would be appreciated. Thanks in advance. MaryAnn Dixon BS Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 392-2235 Ext. 4517 ------------------------------ Message: 3 Date: Thu, 26 Jun 2008 10:53:23 -0700 From: "John Spair" Subject: [Histonet] Formalin Recyling To: histonet@lists.utsouthwestern.edu Message-ID: <61A9977919846C479389493BAE2517CA023760EC@MHSEXMBX1.multicare.org> Content-Type: text/plain; charset=us-ascii Is anyone using the formalin recycling/filtration system sold by Creative Waste Solutions?? If you are , please email me at: john.spair@multicare.org if you don't mind, I have some questions. Thank you. "MMS " made the following annotations. ------------------------------------------------------------------------------ NOTICE: This e-mail and the attachments hereto, if any, may contain privileged and/or confidential information. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, you are hereby notified that any examination, distribution or copying of this e-mail and the attachments hereto, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by email or telephone and permanently delete this e-mail and the attachments hereto, if any, and destroy any printout thereof. MultiCare Health System, Tacoma, WA 98415 (253) 403-1000. ============================================================================== ------------------------------ Message: 4 Date: Thu, 26 Jun 2008 15:10:29 -0500 From: "Jan Shivers" Subject: Re: [Histonet] HMB45 antibody To: "MaryAnn Dixon" , Message-ID: Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original I had little or no success with HMB45 on canine tissue. I use Melan-A exclusively; that works fine. Jan Shivers Senior Scientist Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu ----- Original Message ----- From: "MaryAnn Dixon" To: Sent: Thursday, June 26, 2008 12:56 PM Subject: [Histonet] HMB45 antibody Hi histonetters, Anyone out there successfully using a monoclonal HMB45 antibody on canine tissues? I think I've read a dozen papers all with different protocols and my eyes are about to pop out of my head! Currently I'm at a 1:50 dilution with a pretreatment in a citrate buffer (pressure cooker). I have my choice of a polymer based detection as well as a biotinylated detection. Any help would be appreciated. Thanks in advance. MaryAnn Dixon BS Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 392-2235 Ext. 4517 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Thu, 26 Jun 2008 16:52:01 -0500 From: Atoska Gentry Subject: [Histonet] RE: whole mount cryosections To: Histonet Message-ID: <48640F81.7080301@vetmed.auburn.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed hello, will someone with expertise in fresh frozen whole mount and/or coronal primate brain sections please contact me with pointers on requirements for this type sMicrom HM 505 E that will accommodate such samples? We normally section standard size sections but now one of our PI's is considering trying larger samples. Thanks, Atoska ------------------------------ Message: 6 Date: Thu, 26 Jun 2008 15:52:50 -0700 (PDT) From: "Stephen Peters M.D." Subject: [Histonet] whole mount cryosections To: Histonet@lists.utsouthwestern.edu Message-ID: <567183.62199.qm@web45114.mail.sp1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Atoska, ? Our 50 x 30 mm well bar set? with 55 x 35 mm chucks may give a bit more room to work. See item # 115 on this page: http://pathologyinnovations.com/Price%20List.htm ? Stephen ? ------------------------------ Message: 7 Date: Sat, 26 Jan 2008 22:10:43 -0500 From: "Rebecca Johnson" Subject: [Histonet] Prisma Stainer/coverslipper To: "histonet" Message-ID: <013d01c86092$341481b0$7b48f9d8@CHURCH> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Is anyone using the Prisma coverslipper in Cytology for the Imager? If so how are you doing it? Length of film and level. Thanks Becky ------------------------------ Message: 8 Date: Fri, 27 Jun 2008 09:32:59 -0400 From: Jennifer Johnson Subject: [Histonet] Vacuum pumps To: Message-ID: Content-Type: text/plain; charset="Windows-1252" The James Watson Company sells new & used vacuum pumps. Their phone number is (770) 422-1154. I do not know if they have a web site. They are a direct source instead of having to buy from a medical supply company that will mark up your product. Jennifer Johnson, HTL (ASCP) P.S. I have an affiliation with this company. It is owned by my ex brother-in-law twice removed. _________________________________________________________________ The other season of giving begins 6/24/08. Check out the i?m Talkathon. http://www.imtalkathon.com?source=TXT_EML_WLH_SeasonOfGiving ------------------------------ Message: 9 Date: Fri, 27 Jun 2008 09:42:28 -0400 From: "Connolly, Brett M" Subject: [Histonet] RE: IHC marker for M-phase cells To: , Message-ID: <63EA0607835FBA4689CEA9EA8B48269201306E3A@usctmx1141.merck.com> Content-Type: text/plain; charset="us-ascii" Thanks to all who replied to my question. Seems like everyone uses anti-phosph-histone H3 (Ser10) as I have also used. However, the Ser10 antibody has also been shown to label interphase G2 cells (Goto et al., J Biol Chem 1999, 274: 25543-25549 and others) and I was really looking for an M-phase only marker. So if anyone has used the phospho-Ser28 histone H3 antibody I would be interested to hear your experiences. Brett > _____________________________________________ > From: Connolly, Brett M > Sent: Thursday, June 26, 2008 9:28 AM > To: histonet@lists.utsouthwestern.edu; ihcrg@googlegroups.com > Subject: IHC marker for M-phase cells > > I am looking for an antibody for labeling FFPE sections of mitotic > cells that are only in M-phase. Found one article in J Histo Cyto > using clone HTA28-phospho-Histone H3(Ser 28) that looked pretty good, > but the Authors produced their own antibody. > > The commercial suppliers I have been able to locate do not indicate > IHC as a suitable application for their products. > > Anyone had experience with this?? > > Or can you recommend another M-phase specific antibody? > > Many thanks, > Brett > > Brett M. Connolly, Ph.D. > Research Fellow, Imaging Research > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > PH 215-652-2501 fax. 215-993-6803 > e-mail. brett_connolly@merck.com > Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 10 Date: Fri, 27 Jun 2008 10:17:17 -0600 From: "Patsy Ruegg" Subject: [Histonet] lab tek metal slide cabinets To: "'Histonet'" Message-ID: <005601c8d871$45f86570$6401a8c0@Patsyoffice> Content-Type: text/plain; charset="us-ascii" I have some brand new lab tek metal slide cabinets that are missing the drawer dividers, does anyone know where I can buy these? They turn the drawer into two so you can line up slides or blocks side by side, without these nothing fits in the drawer. Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 55, Issue 40 **************************************** From ruebenjcarter <@t> gmail.com Fri Jun 27 14:10:48 2008 From: ruebenjcarter <@t> gmail.com (R C) Date: Fri Jun 27 14:10:53 2008 Subject: [Histonet] IHC Marker for differentiation of Human to Mouse hepatocytes Message-ID: <2a926e3f0806271210i5c91c94w131a39870892e9f8@mail.gmail.com> Is there a known Ab that exhibits + expression of hepatacytes in humans but not mice? Thank you. Ruben Carter From erin <@t> aaisolutions.com Fri Jun 27 17:50:49 2008 From: erin <@t> aaisolutions.com (Erin Curry) Date: Fri Jun 27 17:59:24 2008 Subject: [Histonet] FlexStation 3 needs new home Message-ID: <005c01c8d8a8$3f42d8f0$bdc88ad0$@com> Hello all, We are looking for a new home for our Molecular Devices FlexStation 3 multimode microplate reader. It was new in 2006 and has liquid transfer capabilities. If anyone is interested please contact me at the email address below. Erin Curry 1460 O'Brien Drive Menlo Park, CA 94025 (650) 324-2569 erin@aaisolutions.com From JimR0712 <@t> comcast.net Fri Jun 27 19:16:26 2008 From: JimR0712 <@t> comcast.net (JimR0712@comcast.net) Date: Fri Jun 27 19:16:29 2008 Subject: [Histonet] MOHS job openings at Loyola University Medical Center Message-ID: <062820080016.19416.486582DA0001C92200004BD82216554886CDCEC9CFAD0307B6@comcast.net> Loyola University Medical has 2 immediate opening for MOHS technicians. These openings are the result of opening a second MOHS facility in LaGrange, Illinois. For full details or to apply online, please go to www.luhs.org/depts/hr or contact Joyce Milewski , HR Manager,at 708-216-9409. Jim Robinson, M.S., HTL/HT (ASCP) Manager, Anatomic Pathology From Jerry <@t> ralambusa.com Fri Jun 27 19:43:45 2008 From: Jerry <@t> ralambusa.com (Jerry Helisek) Date: Fri Jun 27 19:43:52 2008 Subject: [Histonet] RE: slide cabinets, RA Lamb Message-ID: <3855F92002259948A66A8CA2D16E3A4F0B5624@server.ralambusa.com> Patsy, RA Lamb has drawer dividers that would work if you do not have any luck finding those. Thanks and have a great weekend. ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ ------------------------------ Message: 10 Date: Fri, 27 Jun 2008 10:17:17 -0600 From: "Patsy Ruegg" Subject: [Histonet] lab tek metal slide cabinets To: "'Histonet'" Message-ID: <005601c8d871$45f86570$6401a8c0@Patsyoffice> Content-Type: text/plain; charset="us-ascii" I have some brand new lab tek metal slide cabinets that are missing the drawer dividers, does anyone know where I can buy these? They turn the drawer into two so you can line up slides or blocks side by side, without these nothing fits in the drawer. Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 55, Issue 40 **************************************** From FidgenL <@t> shmc.org Fri Jun 27 19:46:09 2008 From: FidgenL <@t> shmc.org (Fidgen, Laura L.) Date: Fri Jun 27 19:46:13 2008 Subject: [Histonet] HT Positions available Message-ID: <6BB8BC4519AAB844B174FC739A679BBC0148C025@IRMEXCH01.irm.inhs.org> Hello Histonetters! Here at Sacred Heart Medical Center in beautiful Spokane, WA we have 3 F/T HT positions available. We have 2 day shifts, 05:00-13:30 and 07:00-15:30, and one evening shift, 15:00-23:30. We offer great wages, benefits, and relocation assistance is available. We have state of the art equipment in a newly constructed laboratory coupled with excellent pathologists, pathologist assistants, and laboratory assistants. For more information please visit the hospital website at www.shmc.org. If interested in any of these positions please contact Debbie Ozust, Employment Specialist, at 509-474-3191. Laura Fidgen, HT(ASCP)cm Histology Supervisor Sacred Heart Medical Center 509-474-4106 From Ryan.Salazar <@t> maccine.com Fri Jun 27 22:17:24 2008 From: Ryan.Salazar <@t> maccine.com (Ryan Salazar) Date: Fri Jun 27 22:10:03 2008 Subject: [Histonet] IHC/ISH Message-ID: Deareast Histopeeps! We're currently upgrading our clinical research facility into the world of IHC and ISH. Can you provide us ANY BASIC/ FULL EQUIPMENTS/ PROTOCOLS needed for us to be functional in these procedures? We're currently running the routine H&E and some special stains and we have the instruments needed for these. We just want to be ready for our first IHC and ISH stuffs. Thank you for your humble help and your big contribution! Ryan S. Important : This message is intended for the recipient(s) addressed above. It may contain privileged and confidential information. If you are not the intended recipient, please delete it from your system. You must not copy, use, disseminate, disclose, retain or reproduce all or any part of the information or any attachments from this communication in any form. Thank you. From barbaraaalbert <@t> yahoo.com Sat Jun 28 10:38:52 2008 From: barbaraaalbert <@t> yahoo.com (Barbara Albert) Date: Sat Jun 28 10:38:57 2008 Subject: [Histonet] slide holders for Lerner Linear Stainer Message-ID: <994234.4916.qm@web63707.mail.re1.yahoo.com> Hi all, Some time ago someone on this list asked if anyone had slide holders for the old Lerner Linerar stainer.? Well, we just moved and a box turned up.? Let me know if you need them and these brand new slide holders are yours. ? Barbara Albert Lead Histotechnologist UCSF Medical Center San Francisco 415-353-1610 From je2 <@t> sanger.ac.uk Mon Jun 30 06:02:57 2008 From: je2 <@t> sanger.ac.uk (Jeanne Estabel) Date: Mon Jun 30 06:03:08 2008 Subject: [Histonet] slide printers Message-ID: <0526C4B4E593154B86E6A8651913C28D0245360E@exchsrv2.internal.sanger.ac.uk> Hi, Can I have some feedback on slide printers? Regards Jeanne Jeanne Estabel, PhD MGP Histologist, Team 109 Wellcome Trust/Sanger Institute Hinxton CB10 1SA Tel: 01223 495338 -- The Wellcome Trust Sanger Institute is operated by Genome Research Limited, a charity registered in England with number 1021457 and a compa ny registered in England with number 2742969, whose registered office is 2 15 Euston Road, London, NW1 2BE. From AFeatherstone <@t> KaleidaHealth.Org Mon Jun 30 06:59:22 2008 From: AFeatherstone <@t> KaleidaHealth.Org (Featherstone, Annette) Date: Mon Jun 30 06:59:31 2008 Subject: [Histonet] Quality assurance In-Reply-To: References: Message-ID: <16A5E67B2A1F714885DEDC2CB68DD6091D79F7@KALEXMB03.KaleidaHealth.org> We collect data from our preclinical (accessioning, registration) and document all registration errors, specimen errors, etc. We collect data from our IHC runs such as controls that do not work, wrong antibodies on tissue, wrong cases cut, tissue falling off the slide etc. We do the same for special stains and H & E's. We document all mislabeled blocks and slides. Annette Featherstone Kaleida Health -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Saturday, June 28, 2008 13:02 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 55, Issue 41 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Quality Assurance Measures (Sarah Kolekamp) 2. IHC Marker for differentiation of Human to Mouse hepatocytes (R C) 3. FlexStation 3 needs new home (Erin Curry) 4. MOHS job openings at Loyola University Medical Center (JimR0712@comcast.net) 5. RE: slide cabinets, RA Lamb (Jerry Helisek) 6. HT Positions available (Fidgen, Laura L.) 7. IHC/ISH (Ryan Salazar) 8. slide holders for Lerner Linear Stainer (Barbara Albert) ---------------------------------------------------------------------- Message: 1 Date: Fri, 27 Jun 2008 13:17:33 -0400 From: "Sarah Kolekamp" Subject: [Histonet] Quality Assurance Measures To: Message-ID: <4864E86D0200006100015432@nodcgwcluster_gw4_server.no.trinity-health.org> Content-Type: text/plain; charset=UTF-8 Is anyone currently running a quality assurance program in their histology lab, if so what do you measure/how? We are tring to develop some standards and would like to have data to run analysis on...any ideas on what data to collect?? Thanks, Sarah Kolekamp Sarah Kolekamp HTL (ASCP) Lead Histotechnologist Saint Mary's Health Care Phone Number (616) 752-6155 Fax Number (616) 774-0628 >>> 06/27/08 1:02 PM >>> Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. IHC marker for M-phase cells (Jean-Martin Lapointe) 2. HMB45 antibody (MaryAnn Dixon) 3. Formalin Recyling (John Spair) 4. Re: HMB45 antibody (Jan Shivers) 5. RE: whole mount cryosections (Atoska Gentry) 6. whole mount cryosections (Stephen Peters M.D.) 7. Prisma Stainer/coverslipper (Rebecca Johnson) 8. Vacuum pumps (Jennifer Johnson) 9. RE: IHC marker for M-phase cells (Connolly, Brett M) 10. lab tek metal slide cabinets (Patsy Ruegg) ---------------------------------------------------------------------- Message: 1 Date: Thu, 26 Jun 2008 13:23:17 -0400 From: "Jean-Martin Lapointe" Subject: [Histonet] IHC marker for M-phase cells To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Brett, at my previous job we did IHC staining for phospho-Histone H3 (ser10) using the Cell Signaling rabbit monoclonal antibody CS9701 in FFPE tissue, and it worked beautifully. It was on human origin tissue, don't remember cross-reactivity in other species but Cell Signaling mentions it reacts with many others. Good luck Jean-Martin __________________________________ Jean-Martin Lapointe, DMV, MS, dACVP Vice-President, Pathologie AccelLAB Inc 1635 Lionel-Bertrand, Boisbriand Qu??bec, Canada J7H 1N8 tel:?? 450-435-9482 ext.247 fax: 450-435-4795 jm.lapointe@accellab.com ?? ?? ________________________________________________________________ Message: 6 Date: Thu, 26 Jun 2008 09:27:58 -0400 From: "Connolly, Brett M" Subject: [Histonet] IHC marker for M-phase cells To: , Message-ID: <63EA0607835FBA4689CEA9EA8B48269201306BD2@usctmx1141.merck.com> Content-Type: text/plain; charset="us-ascii" I am looking for an antibody for labeling FFPE sections of mitotic cells that are only in M-phase. Found one article in J Histo Cyto using clone HTA28-phospho-Histone H3(Ser 28) that looked pretty good, but the Authors produced their own antibody. The commercial suppliers I have been able to locate do not indicate IHC as a suitable application for their products. Anyone had experience with this?? Or can you recommend another M-phase specific antibody? Many thanks, Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Research Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com ******************** ------------------------------ Message: 2 Date: Thu, 26 Jun 2008 13:56:05 -0400 From: "MaryAnn Dixon" Subject: [Histonet] HMB45 antibody To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi histonetters, Anyone out there successfully using a monoclonal HMB45 antibody on canine tissues? I think I've read a dozen papers all with different protocols and my eyes are about to pop out of my head! Currently I'm at a 1:50 dilution with a pretreatment in a citrate buffer (pressure cooker). I have my choice of a polymer based detection as well as a biotinylated detection. Any help would be appreciated. Thanks in advance. MaryAnn Dixon BS Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 392-2235 Ext. 4517 ------------------------------ Message: 3 Date: Thu, 26 Jun 2008 10:53:23 -0700 From: "John Spair" Subject: [Histonet] Formalin Recyling To: histonet@lists.utsouthwestern.edu Message-ID: <61A9977919846C479389493BAE2517CA023760EC@MHSEXMBX1.multicare.org> Content-Type: text/plain; charset=us-ascii Is anyone using the formalin recycling/filtration system sold by Creative Waste Solutions?? If you are , please email me at: john.spair@multicare.org if you don't mind, I have some questions. Thank you. "MMS " made the following annotations. ------------------------------------------------------------------------------ NOTICE: This e-mail and the attachments hereto, if any, may contain privileged and/or confidential information. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, you are hereby notified that any examination, distribution or copying of this e-mail and the attachments hereto, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by email or telephone and permanently delete this e-mail and the attachments hereto, if any, and destroy any printout thereof. MultiCare Health System, Tacoma, WA 98415 (253) 403-1000. ============================================================================== ------------------------------ Message: 4 Date: Thu, 26 Jun 2008 15:10:29 -0500 From: "Jan Shivers" Subject: Re: [Histonet] HMB45 antibody To: "MaryAnn Dixon" , Message-ID: Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original I had little or no success with HMB45 on canine tissue. I use Melan-A exclusively; that works fine. Jan Shivers Senior Scientist Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu ----- Original Message ----- From: "MaryAnn Dixon" To: Sent: Thursday, June 26, 2008 12:56 PM Subject: [Histonet] HMB45 antibody Hi histonetters, Anyone out there successfully using a monoclonal HMB45 antibody on canine tissues? I think I've read a dozen papers all with different protocols and my eyes are about to pop out of my head! Currently I'm at a 1:50 dilution with a pretreatment in a citrate buffer (pressure cooker). I have my choice of a polymer based detection as well as a biotinylated detection. Any help would be appreciated. Thanks in advance. MaryAnn Dixon BS Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 392-2235 Ext. 4517 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Thu, 26 Jun 2008 16:52:01 -0500 From: Atoska Gentry Subject: [Histonet] RE: whole mount cryosections To: Histonet Message-ID: <48640F81.7080301@vetmed.auburn.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed hello, will someone with expertise in fresh frozen whole mount and/or coronal primate brain sections please contact me with pointers on requirements for this type sMicrom HM 505 E that will accommodate such samples? We normally section standard size sections but now one of our PI's is considering trying larger samples. Thanks, Atoska ------------------------------ Message: 6 Date: Thu, 26 Jun 2008 15:52:50 -0700 (PDT) From: "Stephen Peters M.D." Subject: [Histonet] whole mount cryosections To: Histonet@lists.utsouthwestern.edu Message-ID: <567183.62199.qm@web45114.mail.sp1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Atoska, ?? Our 50 x 30 mm well bar set?? with 55 x 35 mm chucks may give a bit more room to work. See item # 115 on this page: http://pathologyinnovations.com/Price%20List.htm ?? Stephen ?? ------------------------------ Message: 7 Date: Sat, 26 Jan 2008 22:10:43 -0500 From: "Rebecca Johnson" Subject: [Histonet] Prisma Stainer/coverslipper To: "histonet" Message-ID: <013d01c86092$341481b0$7b48f9d8@CHURCH> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Is anyone using the Prisma coverslipper in Cytology for the Imager? If so how are you doing it? Length of film and level. Thanks Becky ------------------------------ Message: 8 Date: Fri, 27 Jun 2008 09:32:59 -0400 From: Jennifer Johnson Subject: [Histonet] Vacuum pumps To: Message-ID: Content-Type: text/plain; charset="Windows-1252" The James Watson Company sells new & used vacuum pumps. Their phone number is (770) 422-1154. I do not know if they have a web site. They are a direct source instead of having to buy from a medical supply company that will mark up your product. Jennifer Johnson, HTL (ASCP) P.S. I have an affiliation with this company. It is owned by my ex brother-in-law twice removed. _________________________________________________________________ The other season of giving begins 6/24/08. Check out the i??m Talkathon. http://www.imtalkathon.com?source=TXT_EML_WLH_SeasonOfGiving ------------------------------ Message: 9 Date: Fri, 27 Jun 2008 09:42:28 -0400 From: "Connolly, Brett M" Subject: [Histonet] RE: IHC marker for M-phase cells To: , Message-ID: <63EA0607835FBA4689CEA9EA8B48269201306E3A@usctmx1141.merck.com> Content-Type: text/plain; charset="us-ascii" Thanks to all who replied to my question. Seems like everyone uses anti-phosph-histone H3 (Ser10) as I have also used. However, the Ser10 antibody has also been shown to label interphase G2 cells (Goto et al., J Biol Chem 1999, 274: 25543-25549 and others) and I was really looking for an M-phase only marker. So if anyone has used the phospho-Ser28 histone H3 antibody I would be interested to hear your experiences. Brett > _____________________________________________ > From: Connolly, Brett M > Sent: Thursday, June 26, 2008 9:28 AM > To: histonet@lists.utsouthwestern.edu; ihcrg@googlegroups.com > Subject: IHC marker for M-phase cells > > I am looking for an antibody for labeling FFPE sections of mitotic > cells that are only in M-phase. Found one article in J Histo Cyto > using clone HTA28-phospho-Histone H3(Ser 28) that looked pretty good, > but the Authors produced their own antibody. > > The commercial suppliers I have been able to locate do not indicate > IHC as a suitable application for their products. > > Anyone had experience with this?? > > Or can you recommend another M-phase specific antibody? > > Many thanks, > Brett > > Brett M. Connolly, Ph.D. > Research Fellow, Imaging Research > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > PH 215-652-2501 fax. 215-993-6803 > e-mail. brett_connolly@merck.com > Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 10 Date: Fri, 27 Jun 2008 10:17:17 -0600 From: "Patsy Ruegg" Subject: [Histonet] lab tek metal slide cabinets To: "'Histonet'" Message-ID: <005601c8d871$45f86570$6401a8c0@Patsyoffice> Content-Type: text/plain; charset="us-ascii" I have some brand new lab tek metal slide cabinets that are missing the drawer dividers, does anyone know where I can buy these? They turn the drawer into two so you can line up slides or blocks side by side, without these nothing fits in the drawer. Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 55, Issue 40 **************************************** ------------------------------ Message: 2 Date: Fri, 27 Jun 2008 12:10:48 -0700 From: "R C" Subject: [Histonet] IHC Marker for differentiation of Human to Mouse hepatocytes To: histonet@lists.utsouthwestern.edu Message-ID: <2a926e3f0806271210i5c91c94w131a39870892e9f8@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Is there a known Ab that exhibits + expression of hepatacytes in humans but not mice? Thank you. Ruben Carter ------------------------------ Message: 3 Date: Fri, 27 Jun 2008 15:50:49 -0700 From: "Erin Curry" Subject: [Histonet] FlexStation 3 needs new home To: Message-ID: <005c01c8d8a8$3f42d8f0$bdc88ad0$@com> Content-Type: text/plain; charset="us-ascii" Hello all, We are looking for a new home for our Molecular Devices FlexStation 3 multimode microplate reader. It was new in 2006 and has liquid transfer capabilities. If anyone is interested please contact me at the email address below. Erin Curry 1460 O'Brien Drive Menlo Park, CA 94025 (650) 324-2569 erin@aaisolutions.com ------------------------------ Message: 4 Date: Sat, 28 Jun 2008 00:16:26 +0000 From: JimR0712@comcast.net Subject: [Histonet] MOHS job openings at Loyola University Medical Center To: histonet@lists.utsouthwestern.edu (Histonet2), histonet-request@lists.utsouthwestern.edu (Histonet) Message-ID: <062820080016.19416.486582DA0001C92200004BD82216554886CDCEC9CFAD0307B6@comcast.net> Loyola University Medical has 2 immediate opening for MOHS technicians. These openings are the result of opening a second MOHS facility in LaGrange, Illinois. For full details or to apply online, please go to www.luhs.org/depts/hr or contact Joyce Milewski , HR Manager,at 708-216-9409. Jim Robinson, M.S., HTL/HT (ASCP) Manager, Anatomic Pathology ------------------------------ Message: 5 Date: Fri, 27 Jun 2008 20:43:45 -0400 From: "Jerry Helisek" Subject: [Histonet] RE: slide cabinets, RA Lamb To: , Message-ID: <3855F92002259948A66A8CA2D16E3A4F0B5624@server.ralambusa.com> Content-Type: text/plain; charset="us-ascii" Patsy, RA Lamb has drawer dividers that would work if you do not have any luck finding those. Thanks and have a great weekend. ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ ------------------------------ Message: 10 Date: Fri, 27 Jun 2008 10:17:17 -0600 From: "Patsy Ruegg" Subject: [Histonet] lab tek metal slide cabinets To: "'Histonet'" Message-ID: <005601c8d871$45f86570$6401a8c0@Patsyoffice> Content-Type: text/plain; charset="us-ascii" I have some brand new lab tek metal slide cabinets that are missing the drawer dividers, does anyone know where I can buy these? They turn the drawer into two so you can line up slides or blocks side by side, without these nothing fits in the drawer. Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 55, Issue 40 **************************************** ------------------------------ Message: 6 Date: Fri, 27 Jun 2008 17:46:09 -0700 From: "Fidgen, Laura L." Subject: [Histonet] HT Positions available To: Message-ID: <6BB8BC4519AAB844B174FC739A679BBC0148C025@IRMEXCH01.irm.inhs.org> Content-Type: text/plain; charset="iso-8859-1" Hello Histonetters! Here at Sacred Heart Medical Center in beautiful Spokane, WA we have 3 F/T HT positions available. We have 2 day shifts, 05:00-13:30 and 07:00-15:30, and one evening shift, 15:00-23:30. We offer great wages, benefits, and relocation assistance is available. We have state of the art equipment in a newly constructed laboratory coupled with excellent pathologists, pathologist assistants, and laboratory assistants. For more information please visit the hospital website at www.shmc.org. If interested in any of these positions please contact Debbie Ozust, Employment Specialist, at 509-474-3191. Laura Fidgen, HT(ASCP)cm Histology Supervisor Sacred Heart Medical Center 509-474-4106 ------------------------------ Message: 7 Date: Sat, 28 Jun 2008 11:17:24 +0800 From: "Ryan Salazar" Subject: [Histonet] IHC/ISH To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Deareast Histopeeps! We're currently upgrading our clinical research facility into the world of IHC and ISH. Can you provide us ANY BASIC/ FULL EQUIPMENTS/ PROTOCOLS needed for us to be functional in these procedures? We're currently running the routine H&E and some special stains and we have the instruments needed for these. We just want to be ready for our first IHC and ISH stuffs. Thank you for your humble help and your big contribution! Ryan S. Important : This message is intended for the recipient(s) addressed above. It may contain privileged and confidential information. If you are not the intended recipient, please delete it from your system. You must not copy, use, disseminate, disclose, retain or reproduce all or any part of the information or any attachments from this communication in any form. Thank you. ------------------------------ Message: 8 Date: Sat, 28 Jun 2008 08:38:52 -0700 (PDT) From: Barbara Albert Subject: [Histonet] slide holders for Lerner Linear Stainer To: histonet Message-ID: <994234.4916.qm@web63707.mail.re1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi all, Some time ago someone on this list asked if anyone had slide holders for the old Lerner Linerar stainer.? Well, we just moved and a box turned up.? Let me know if you need them and these brand new slide holders are yours. ? Barbara Albert Lead Histotechnologist UCSF Medical Center San Francisco 415-353-1610 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 55, Issue 41 **************************************** 2007 Best Places to Work Finalist Visit our careers page at www.kaleidahealth.org/careers CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From rjbuesa <@t> yahoo.com Mon Jun 30 07:05:43 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jun 30 07:05:54 2008 Subject: [Histonet] Quality assurance In-Reply-To: <16A5E67B2A1F714885DEDC2CB68DD6091D79F7@KALEXMB03.KaleidaHealth.org> Message-ID: <860432.64776.qm@web65710.mail.ac4.yahoo.com> We also used to record when the FS and permanent diagnosis did not completely match, and when the size of the permanent was of only about 50% of the FS section size. Ren? J. --- On Mon, 6/30/08, Featherstone, Annette wrote: From: Featherstone, Annette Subject: [Histonet] Quality assurance To: histonet@lists.utsouthwestern.edu Date: Monday, June 30, 2008, 7:59 AM We collect data from our preclinical (accessioning, registration) and document all registration errors, specimen errors, etc. We collect data from our IHC runs such as controls that do not work, wrong antibodies on tissue, wrong cases cut, tissue falling off the slide etc. We do the same for special stains and H & E's. We document all mislabeled blocks and slides. Annette Featherstone Kaleida Health -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Saturday, June 28, 2008 13:02 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 55, Issue 41 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Quality Assurance Measures (Sarah Kolekamp) 2. IHC Marker for differentiation of Human to Mouse hepatocytes (R C) 3. FlexStation 3 needs new home (Erin Curry) 4. MOHS job openings at Loyola University Medical Center (JimR0712@comcast.net) 5. RE: slide cabinets, RA Lamb (Jerry Helisek) 6. HT Positions available (Fidgen, Laura L.) 7. IHC/ISH (Ryan Salazar) 8. slide holders for Lerner Linear Stainer (Barbara Albert) ---------------------------------------------------------------------- Message: 1 Date: Fri, 27 Jun 2008 13:17:33 -0400 From: "Sarah Kolekamp" Subject: [Histonet] Quality Assurance Measures To: Message-ID: <4864E86D0200006100015432@nodcgwcluster_gw4_server.no.trinity-health.org> Content-Type: text/plain; charset=UTF-8 Is anyone currently running a quality assurance program in their histology lab, if so what do you measure/how? We are tring to develop some standards and would like to have data to run analysis on...any ideas on what data to collect?? Thanks, Sarah Kolekamp Sarah Kolekamp HTL (ASCP) Lead Histotechnologist Saint Mary's Health Care Phone Number (616) 752-6155 Fax Number (616) 774-0628 >>> 06/27/08 1:02 PM >>> Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. IHC marker for M-phase cells (Jean-Martin Lapointe) 2. HMB45 antibody (MaryAnn Dixon) 3. Formalin Recyling (John Spair) 4. Re: HMB45 antibody (Jan Shivers) 5. RE: whole mount cryosections (Atoska Gentry) 6. whole mount cryosections (Stephen Peters M.D.) 7. Prisma Stainer/coverslipper (Rebecca Johnson) 8. Vacuum pumps (Jennifer Johnson) 9. RE: IHC marker for M-phase cells (Connolly, Brett M) 10. lab tek metal slide cabinets (Patsy Ruegg) ---------------------------------------------------------------------- Message: 1 Date: Thu, 26 Jun 2008 13:23:17 -0400 From: "Jean-Martin Lapointe" Subject: [Histonet] IHC marker for M-phase cells To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Brett, at my previous job we did IHC staining for phospho-Histone H3 (ser10) using the Cell Signaling rabbit monoclonal antibody CS9701 in FFPE tissue, and it worked beautifully. It was on human origin tissue, don't remember cross-reactivity in other species but Cell Signaling mentions it reacts with many others. Good luck Jean-Martin __________________________________ Jean-Martin Lapointe, DMV, MS, dACVP Vice-President, Pathologie AccelLAB Inc 1635 Lionel-Bertrand, Boisbriand Qu??bec, Canada J7H 1N8 tel:?? 450-435-9482 ext.247 fax: 450-435-4795 jm.lapointe@accellab.com ?? ?? ________________________________________________________________ Message: 6 Date: Thu, 26 Jun 2008 09:27:58 -0400 From: "Connolly, Brett M" Subject: [Histonet] IHC marker for M-phase cells To: , Message-ID: <63EA0607835FBA4689CEA9EA8B48269201306BD2@usctmx1141.merck.com> Content-Type: text/plain; charset="us-ascii" I am looking for an antibody for labeling FFPE sections of mitotic cells that are only in M-phase. Found one article in J Histo Cyto using clone HTA28-phospho-Histone H3(Ser 28) that looked pretty good, but the Authors produced their own antibody. The commercial suppliers I have been able to locate do not indicate IHC as a suitable application for their products. Anyone had experience with this?? Or can you recommend another M-phase specific antibody? Many thanks, Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Research Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com ******************** ------------------------------ Message: 2 Date: Thu, 26 Jun 2008 13:56:05 -0400 From: "MaryAnn Dixon" Subject: [Histonet] HMB45 antibody To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi histonetters, Anyone out there successfully using a monoclonal HMB45 antibody on canine tissues? I think I've read a dozen papers all with different protocols and my eyes are about to pop out of my head! Currently I'm at a 1:50 dilution with a pretreatment in a citrate buffer (pressure cooker). I have my choice of a polymer based detection as well as a biotinylated detection. Any help would be appreciated. Thanks in advance. MaryAnn Dixon BS Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 392-2235 Ext. 4517 ------------------------------ Message: 3 Date: Thu, 26 Jun 2008 10:53:23 -0700 From: "John Spair" Subject: [Histonet] Formalin Recyling To: histonet@lists.utsouthwestern.edu Message-ID: <61A9977919846C479389493BAE2517CA023760EC@MHSEXMBX1.multicare.org> Content-Type: text/plain; charset=us-ascii Is anyone using the formalin recycling/filtration system sold by Creative Waste Solutions?? If you are , please email me at: john.spair@multicare.org if you don't mind, I have some questions. Thank you. "MMS " made the following annotations. ------------------------------------------------------------------------------ NOTICE: This e-mail and the attachments hereto, if any, may contain privileged and/or confidential information. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, you are hereby notified that any examination, distribution or copying of this e-mail and the attachments hereto, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by email or telephone and permanently delete this e-mail and the attachments hereto, if any, and destroy any printout thereof. MultiCare Health System, Tacoma, WA 98415 (253) 403-1000. ============================================================================== ------------------------------ Message: 4 Date: Thu, 26 Jun 2008 15:10:29 -0500 From: "Jan Shivers" Subject: Re: [Histonet] HMB45 antibody To: "MaryAnn Dixon" , Message-ID: Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original I had little or no success with HMB45 on canine tissue. I use Melan-A exclusively; that works fine. Jan Shivers Senior Scientist Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu ----- Original Message ----- From: "MaryAnn Dixon" To: Sent: Thursday, June 26, 2008 12:56 PM Subject: [Histonet] HMB45 antibody Hi histonetters, Anyone out there successfully using a monoclonal HMB45 antibody on canine tissues? I think I've read a dozen papers all with different protocols and my eyes are about to pop out of my head! Currently I'm at a 1:50 dilution with a pretreatment in a citrate buffer (pressure cooker). I have my choice of a polymer based detection as well as a biotinylated detection. Any help would be appreciated. Thanks in advance. MaryAnn Dixon BS Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 392-2235 Ext. 4517 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Thu, 26 Jun 2008 16:52:01 -0500 From: Atoska Gentry Subject: [Histonet] RE: whole mount cryosections To: Histonet Message-ID: <48640F81.7080301@vetmed.auburn.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed hello, will someone with expertise in fresh frozen whole mount and/or coronal primate brain sections please contact me with pointers on requirements for this type sMicrom HM 505 E that will accommodate such samples? We normally section standard size sections but now one of our PI's is considering trying larger samples. Thanks, Atoska ------------------------------ Message: 6 Date: Thu, 26 Jun 2008 15:52:50 -0700 (PDT) From: "Stephen Peters M.D." Subject: [Histonet] whole mount cryosections To: Histonet@lists.utsouthwestern.edu Message-ID: <567183.62199.qm@web45114.mail.sp1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Atoska, ?? Our 50 x 30 mm well bar set?? with 55 x 35 mm chucks may give a bit more room to work. See item # 115 on this page: http://pathologyinnovations.com/Price%20List.htm ?? Stephen ?? ------------------------------ Message: 7 Date: Sat, 26 Jan 2008 22:10:43 -0500 From: "Rebecca Johnson" Subject: [Histonet] Prisma Stainer/coverslipper To: "histonet" Message-ID: <013d01c86092$341481b0$7b48f9d8@CHURCH> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Is anyone using the Prisma coverslipper in Cytology for the Imager? If so how are you doing it? Length of film and level. Thanks Becky ------------------------------ Message: 8 Date: Fri, 27 Jun 2008 09:32:59 -0400 From: Jennifer Johnson Subject: [Histonet] Vacuum pumps To: Message-ID: Content-Type: text/plain; charset="Windows-1252" The James Watson Company sells new & used vacuum pumps. Their phone number is (770) 422-1154. I do not know if they have a web site. They are a direct source instead of having to buy from a medical supply company that will mark up your product. Jennifer Johnson, HTL (ASCP) P.S. I have an affiliation with this company. It is owned by my ex brother-in-law twice removed. _________________________________________________________________ The other season of giving begins 6/24/08. Check out the i??m Talkathon. http://www.imtalkathon.com?source=TXT_EML_WLH_SeasonOfGiving ------------------------------ Message: 9 Date: Fri, 27 Jun 2008 09:42:28 -0400 From: "Connolly, Brett M" Subject: [Histonet] RE: IHC marker for M-phase cells To: , Message-ID: <63EA0607835FBA4689CEA9EA8B48269201306E3A@usctmx1141.merck.com> Content-Type: text/plain; charset="us-ascii" Thanks to all who replied to my question. Seems like everyone uses anti-phosph-histone H3 (Ser10) as I have also used. However, the Ser10 antibody has also been shown to label interphase G2 cells (Goto et al., J Biol Chem 1999, 274: 25543-25549 and others) and I was really looking for an M-phase only marker. So if anyone has used the phospho-Ser28 histone H3 antibody I would be interested to hear your experiences. Brett > _____________________________________________ > From: Connolly, Brett M > Sent: Thursday, June 26, 2008 9:28 AM > To: histonet@lists.utsouthwestern.edu; ihcrg@googlegroups.com > Subject: IHC marker for M-phase cells > > I am looking for an antibody for labeling FFPE sections of mitotic > cells that are only in M-phase. Found one article in J Histo Cyto > using clone HTA28-phospho-Histone H3(Ser 28) that looked pretty good, > but the Authors produced their own antibody. > > The commercial suppliers I have been able to locate do not indicate > IHC as a suitable application for their products. > > Anyone had experience with this?? > > Or can you recommend another M-phase specific antibody? > > Many thanks, > Brett > > Brett M. Connolly, Ph.D. > Research Fellow, Imaging Research > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > PH 215-652-2501 fax. 215-993-6803 > e-mail. brett_connolly@merck.com > Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 10 Date: Fri, 27 Jun 2008 10:17:17 -0600 From: "Patsy Ruegg" Subject: [Histonet] lab tek metal slide cabinets To: "'Histonet'" Message-ID: <005601c8d871$45f86570$6401a8c0@Patsyoffice> Content-Type: text/plain; charset="us-ascii" I have some brand new lab tek metal slide cabinets that are missing the drawer dividers, does anyone know where I can buy these? They turn the drawer into two so you can line up slides or blocks side by side, without these nothing fits in the drawer. Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 55, Issue 40 **************************************** ------------------------------ Message: 2 Date: Fri, 27 Jun 2008 12:10:48 -0700 From: "R C" Subject: [Histonet] IHC Marker for differentiation of Human to Mouse hepatocytes To: histonet@lists.utsouthwestern.edu Message-ID: <2a926e3f0806271210i5c91c94w131a39870892e9f8@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Is there a known Ab that exhibits + expression of hepatacytes in humans but not mice? Thank you. Ruben Carter ------------------------------ Message: 3 Date: Fri, 27 Jun 2008 15:50:49 -0700 From: "Erin Curry" Subject: [Histonet] FlexStation 3 needs new home To: Message-ID: <005c01c8d8a8$3f42d8f0$bdc88ad0$@com> Content-Type: text/plain; charset="us-ascii" Hello all, We are looking for a new home for our Molecular Devices FlexStation 3 multimode microplate reader. It was new in 2006 and has liquid transfer capabilities. If anyone is interested please contact me at the email address below. Erin Curry 1460 O'Brien Drive Menlo Park, CA 94025 (650) 324-2569 erin@aaisolutions.com ------------------------------ Message: 4 Date: Sat, 28 Jun 2008 00:16:26 +0000 From: JimR0712@comcast.net Subject: [Histonet] MOHS job openings at Loyola University Medical Center To: histonet@lists.utsouthwestern.edu (Histonet2), histonet-request@lists.utsouthwestern.edu (Histonet) Message-ID: <062820080016.19416.486582DA0001C92200004BD82216554886CDCEC9CFAD0307B6@comcast.net> Loyola University Medical has 2 immediate opening for MOHS technicians. These openings are the result of opening a second MOHS facility in LaGrange, Illinois. For full details or to apply online, please go to www.luhs.org/depts/hr or contact Joyce Milewski , HR Manager,at 708-216-9409. Jim Robinson, M.S., HTL/HT (ASCP) Manager, Anatomic Pathology ------------------------------ Message: 5 Date: Fri, 27 Jun 2008 20:43:45 -0400 From: "Jerry Helisek" Subject: [Histonet] RE: slide cabinets, RA Lamb To: , Message-ID: <3855F92002259948A66A8CA2D16E3A4F0B5624@server.ralambusa.com> Content-Type: text/plain; charset="us-ascii" Patsy, RA Lamb has drawer dividers that would work if you do not have any luck finding those. Thanks and have a great weekend. ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ ------------------------------ Message: 10 Date: Fri, 27 Jun 2008 10:17:17 -0600 From: "Patsy Ruegg" Subject: [Histonet] lab tek metal slide cabinets To: "'Histonet'" Message-ID: <005601c8d871$45f86570$6401a8c0@Patsyoffice> Content-Type: text/plain; charset="us-ascii" I have some brand new lab tek metal slide cabinets that are missing the drawer dividers, does anyone know where I can buy these? They turn the drawer into two so you can line up slides or blocks side by side, without these nothing fits in the drawer. Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 55, Issue 40 **************************************** ------------------------------ Message: 6 Date: Fri, 27 Jun 2008 17:46:09 -0700 From: "Fidgen, Laura L." Subject: [Histonet] HT Positions available To: Message-ID: <6BB8BC4519AAB844B174FC739A679BBC0148C025@IRMEXCH01.irm.inhs.org> Content-Type: text/plain; charset="iso-8859-1" Hello Histonetters! Here at Sacred Heart Medical Center in beautiful Spokane, WA we have 3 F/T HT positions available. We have 2 day shifts, 05:00-13:30 and 07:00-15:30, and one evening shift, 15:00-23:30. We offer great wages, benefits, and relocation assistance is available. We have state of the art equipment in a newly constructed laboratory coupled with excellent pathologists, pathologist assistants, and laboratory assistants. For more information please visit the hospital website at www.shmc.org. If interested in any of these positions please contact Debbie Ozust, Employment Specialist, at 509-474-3191. Laura Fidgen, HT(ASCP)cm Histology Supervisor Sacred Heart Medical Center 509-474-4106 ------------------------------ Message: 7 Date: Sat, 28 Jun 2008 11:17:24 +0800 From: "Ryan Salazar" Subject: [Histonet] IHC/ISH To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Deareast Histopeeps! We're currently upgrading our clinical research facility into the world of IHC and ISH. Can you provide us ANY BASIC/ FULL EQUIPMENTS/ PROTOCOLS needed for us to be functional in these procedures? We're currently running the routine H&E and some special stains and we have the instruments needed for these. We just want to be ready for our first IHC and ISH stuffs. Thank you for your humble help and your big contribution! Ryan S. Important : This message is intended for the recipient(s) addressed above. It may contain privileged and confidential information. If you are not the intended recipient, please delete it from your system. You must not copy, use, disseminate, disclose, retain or reproduce all or any part of the information or any attachments from this communication in any form. Thank you. ------------------------------ Message: 8 Date: Sat, 28 Jun 2008 08:38:52 -0700 (PDT) From: Barbara Albert Subject: [Histonet] slide holders for Lerner Linear Stainer To: histonet Message-ID: <994234.4916.qm@web63707.mail.re1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi all, Some time ago someone on this list asked if anyone had slide holders for the old Lerner Linerar stainer.? Well, we just moved and a box turned up.? Let me know if you need them and these brand new slide holders are yours. ? Barbara Albert Lead Histotechnologist UCSF Medical Center San Francisco 415-353-1610 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 55, Issue 41 **************************************** 2007 Best Places to Work Finalist Visit our careers page at www.kaleidahealth.org/careers CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abijag76 <@t> yahoo.co.in Mon Jun 30 08:34:16 2008 From: abijag76 <@t> yahoo.co.in (abi jag) Date: Mon Jun 30 08:34:24 2008 Subject: [Histonet] staining manual reg Message-ID: <469154.7181.qm@web95106.mail.in2.yahoo.com> Dear Histonetters, I am working as a?histologist in one of the pharmaceutical companies in India. I have accepted an opportunity to set up a histology lab(mainly rodent tissues). I have an query. ? In our archives, i have found some references about Richard Allan website staining manual which is having very useful information for trouble shooting. ? But i didn't find any staining manual and also? Richard Allan is now a part of thermo. ? If any of our fellow histonetters have any idea about this staining manual or its contents. ? thanks for all your help ? ABI ? ? ? ? Explore your hobbies and interests. Go to http://in.promos.yahoo.com/groups/ From mike <@t> pathview.com Mon Jun 30 09:20:23 2008 From: mike <@t> pathview.com (Michael Mihalik) Date: Mon Jun 30 09:20:46 2008 Subject: [Histonet] slide printers In-Reply-To: <0526C4B4E593154B86E6A8651913C28D0245360E@exchsrv2.internal.sanger.ac.uk> References: <0526C4B4E593154B86E6A8651913C28D0245360E@exchsrv2.internal.sanger.ac.uk> Message-ID: <6D3D5A3D481A49ABA9A78EAAD1DED567@MDMM1330> Good morning, You may want to check the archives as there have been a few postings on this subject recently. Depending on your usage, you may want to consider slide LABEL printers rather than slide etchers. In our application, we need the label/slide to be generated just prior to the histotechnologist cutting the tissue. Because this requires a slide printer/labeler to be next to each microtome and because we need these slides/labels to print very quickly, we chose to go with a label printer rather than an etcher. If you would like some details on the printer or would like to hear further details, please let me know. Michael Mihalik PathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeanne Estabel Sent: Monday, June 30, 2008 4:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slide printers Hi, Can I have some feedback on slide printers? Regards Jeanne Jeanne Estabel, PhD MGP Histologist, Team 109 Wellcome Trust/Sanger Institute Hinxton CB10 1SA Tel: 01223 495338 -- The Wellcome Trust Sanger Institute is operated by Genome Research Limited, a charity registered in England with number 1021457 and a compa ny registered in England with number 2742969, whose registered office is 2 15 Euston Road, London, NW1 2BE. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hhernandez <@t> pathreflab.com Mon Jun 30 10:56:03 2008 From: hhernandez <@t> pathreflab.com (Hector Hernandez) Date: Mon Jun 30 10:55:29 2008 Subject: [Histonet] HT Positions available Message-ID: Hello Histonetters! Currently PRL (Pathology Reference Lab) is looking for 3 Histology positions to fill. Two will be day shift positions and one will be an evening position 5PM-1:30AM at a satellite lab. We offer excellent benefits and wages will depend on your experience. We are located in warm-hot San Antonio Texas. If interested in any of these positions contact Hector Hernandez at (210)892-3747. Hector Hernandez, HT (ASCP) Histology Supervisor 210-892-3747 From KKay <@t> chr.ab.ca Mon Jun 30 12:17:54 2008 From: KKay <@t> chr.ab.ca (Kay, Karen) Date: Mon Jun 30 12:18:03 2008 Subject: [Histonet] HISTOLOGY QUALITY ASSURANCE RE: Histonet Digest, Vol 55, Issue 42 In-Reply-To: Message-ID: <9C0BD812BAB0BA4DB7E7FD4F5FA3CAA80A494BA3@exbe.chr.ab.ca> Hi Sarah, We document control results for all special stains, IHC, and routine H&E. In addition we also track all occurrences, i.e. milabelled slides, blocks, specimen accessioning issues, tissue pick-up on wrong slides, tissue floaters, dictation issues and any injuries. Also number of days within a defined period where work is not completed. We also run and track and extensive QA program from the pathologist's perspective with parameters set as indicated by the consulting pathologist for the area. Karen Kay Chinook Health Region Laboratory Lethbridge, Alberta, CANADA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: June 30, 2008 11:09 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 55, Issue 42 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. slide printers (Jeanne Estabel) 2. Quality assurance (Featherstone, Annette) 3. Re: Quality assurance (Rene J Buesa) 4. staining manual reg (abi jag) 5. RE: slide printers (Michael Mihalik) 6. HT Positions available (Hector Hernandez) ---------------------------------------------------------------------- Message: 1 Date: Mon, 30 Jun 2008 12:02:57 +0100 From: "Jeanne Estabel" Subject: [Histonet] slide printers To: Message-ID: <0526C4B4E593154B86E6A8651913C28D0245360E@exchsrv2.internal.sanger.ac.uk> Content-Type: text/plain; charset="us-ascii" Hi, Can I have some feedback on slide printers? Regards Jeanne Jeanne Estabel, PhD MGP Histologist, Team 109 Wellcome Trust/Sanger Institute Hinxton CB10 1SA Tel: 01223 495338 -- The Wellcome Trust Sanger Institute is operated by Genome Research Limited, a charity registered in England with number 1021457 and a compa ny registered in England with number 2742969, whose registered office is 2 15 Euston Road, London, NW1 2BE. ------------------------------ Message: 2 Date: Mon, 30 Jun 2008 07:59:22 -0400 From: "Featherstone, Annette" Subject: [Histonet] Quality assurance To: Message-ID: <16A5E67B2A1F714885DEDC2CB68DD6091D79F7@KALEXMB03.KaleidaHealth.org> Content-Type: text/plain; charset="utf-8" We collect data from our preclinical (accessioning, registration) and document all registration errors, specimen errors, etc. We collect data from our IHC runs such as controls that do not work, wrong antibodies on tissue, wrong cases cut, tissue falling off the slide etc. We do the same for special stains and H & E's. We document all mislabeled blocks and slides. Annette Featherstone Kaleida Health -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Saturday, June 28, 2008 13:02 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 55, Issue 41 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Quality Assurance Measures (Sarah Kolekamp) 2. IHC Marker for differentiation of Human to Mouse hepatocytes (R C) 3. FlexStation 3 needs new home (Erin Curry) 4. MOHS job openings at Loyola University Medical Center (JimR0712@comcast.net) 5. RE: slide cabinets, RA Lamb (Jerry Helisek) 6. HT Positions available (Fidgen, Laura L.) 7. IHC/ISH (Ryan Salazar) 8. slide holders for Lerner Linear Stainer (Barbara Albert) ---------------------------------------------------------------------- Message: 1 Date: Fri, 27 Jun 2008 13:17:33 -0400 From: "Sarah Kolekamp" Subject: [Histonet] Quality Assurance Measures To: Message-ID: <4864E86D0200006100015432@nodcgwcluster_gw4_server.no.trinity-health.org> Content-Type: text/plain; charset=UTF-8 Is anyone currently running a quality assurance program in their histology lab, if so what do you measure/how? We are tring to develop some standards and would like to have data to run analysis on...any ideas on what data to collect?? Thanks, Sarah Kolekamp Sarah Kolekamp HTL (ASCP) Lead Histotechnologist Saint Mary's Health Care Phone Number (616) 752-6155 Fax Number (616) 774-0628 >>> 06/27/08 1:02 PM >>> Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. IHC marker for M-phase cells (Jean-Martin Lapointe) 2. HMB45 antibody (MaryAnn Dixon) 3. Formalin Recyling (John Spair) 4. Re: HMB45 antibody (Jan Shivers) 5. RE: whole mount cryosections (Atoska Gentry) 6. whole mount cryosections (Stephen Peters M.D.) 7. Prisma Stainer/coverslipper (Rebecca Johnson) 8. Vacuum pumps (Jennifer Johnson) 9. RE: IHC marker for M-phase cells (Connolly, Brett M) 10. lab tek metal slide cabinets (Patsy Ruegg) ---------------------------------------------------------------------- Message: 1 Date: Thu, 26 Jun 2008 13:23:17 -0400 From: "Jean-Martin Lapointe" Subject: [Histonet] IHC marker for M-phase cells To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Brett, at my previous job we did IHC staining for phospho-Histone H3 (ser10) using the Cell Signaling rabbit monoclonal antibody CS9701 in FFPE tissue, and it worked beautifully. It was on human origin tissue, don't remember cross-reactivity in other species but Cell Signaling mentions it reacts with many others. Good luck Jean-Martin __________________________________ Jean-Martin Lapointe, DMV, MS, dACVP Vice-President, Pathologie AccelLAB Inc 1635 Lionel-Bertrand, Boisbriand Qu????bec, Canada J7H 1N8 tel:???? 450-435-9482 ext.247 fax: 450-435-4795 jm.lapointe@accellab.com ???? ???? ________________________________________________________________ Message: 6 Date: Thu, 26 Jun 2008 09:27:58 -0400 From: "Connolly, Brett M" Subject: [Histonet] IHC marker for M-phase cells To: , Message-ID: <63EA0607835FBA4689CEA9EA8B48269201306BD2@usctmx1141.merck.com> Content-Type: text/plain; charset="us-ascii" I am looking for an antibody for labeling FFPE sections of mitotic cells that are only in M-phase. Found one article in J Histo Cyto using clone HTA28-phospho-Histone H3(Ser 28) that looked pretty good, but the Authors produced their own antibody. The commercial suppliers I have been able to locate do not indicate IHC as a suitable application for their products. Anyone had experience with this?? Or can you recommend another M-phase specific antibody? Many thanks, Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Research Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com ******************** ------------------------------ Message: 2 Date: Thu, 26 Jun 2008 13:56:05 -0400 From: "MaryAnn Dixon" Subject: [Histonet] HMB45 antibody To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi histonetters, Anyone out there successfully using a monoclonal HMB45 antibody on canine tissues? I think I've read a dozen papers all with different protocols and my eyes are about to pop out of my head! Currently I'm at a 1:50 dilution with a pretreatment in a citrate buffer (pressure cooker). I have my choice of a polymer based detection as well as a biotinylated detection. Any help would be appreciated. Thanks in advance. MaryAnn Dixon BS Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 392-2235 Ext. 4517 ------------------------------ Message: 3 Date: Thu, 26 Jun 2008 10:53:23 -0700 From: "John Spair" Subject: [Histonet] Formalin Recyling To: histonet@lists.utsouthwestern.edu Message-ID: <61A9977919846C479389493BAE2517CA023760EC@MHSEXMBX1.multicare.org> Content-Type: text/plain; charset=us-ascii Is anyone using the formalin recycling/filtration system sold by Creative Waste Solutions?? If you are , please email me at: john.spair@multicare.org if you don't mind, I have some questions. Thank you. "MMS " made the following annotations. ------------------------------------------------------------------------------ NOTICE: This e-mail and the attachments hereto, if any, may contain privileged and/or confidential information. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, you are hereby notified that any examination, distribution or copying of this e-mail and the attachments hereto, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by email or telephone and permanently delete this e-mail and the attachments hereto, if any, and destroy any printout thereof. MultiCare Health System, Tacoma, WA 98415 (253) 403-1000. ============================================================================== ------------------------------ Message: 4 Date: Thu, 26 Jun 2008 15:10:29 -0500 From: "Jan Shivers" Subject: Re: [Histonet] HMB45 antibody To: "MaryAnn Dixon" , Message-ID: Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original I had little or no success with HMB45 on canine tissue. I use Melan-A exclusively; that works fine. Jan Shivers Senior Scientist Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu ----- Original Message ----- From: "MaryAnn Dixon" To: Sent: Thursday, June 26, 2008 12:56 PM Subject: [Histonet] HMB45 antibody Hi histonetters, Anyone out there successfully using a monoclonal HMB45 antibody on canine tissues? I think I've read a dozen papers all with different protocols and my eyes are about to pop out of my head! Currently I'm at a 1:50 dilution with a pretreatment in a citrate buffer (pressure cooker). I have my choice of a polymer based detection as well as a biotinylated detection. Any help would be appreciated. Thanks in advance. MaryAnn Dixon BS Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 392-2235 Ext. 4517 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Thu, 26 Jun 2008 16:52:01 -0500 From: Atoska Gentry Subject: [Histonet] RE: whole mount cryosections To: Histonet Message-ID: <48640F81.7080301@vetmed.auburn.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed hello, will someone with expertise in fresh frozen whole mount and/or coronal primate brain sections please contact me with pointers on requirements for this type sMicrom HM 505 E that will accommodate such samples? We normally section standard size sections but now one of our PI's is considering trying larger samples. Thanks, Atoska ------------------------------ Message: 6 Date: Thu, 26 Jun 2008 15:52:50 -0700 (PDT) From: "Stephen Peters M.D." Subject: [Histonet] whole mount cryosections To: Histonet@lists.utsouthwestern.edu Message-ID: <567183.62199.qm@web45114.mail.sp1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Atoska, ???? Our 50 x 30 mm well bar set???? with 55 x 35 mm chucks may give a bit more room to work. See item # 115 on this page: http://pathologyinnovations.com/Price%20List.htm ???? Stephen ???? ------------------------------ Message: 7 Date: Sat, 26 Jan 2008 22:10:43 -0500 From: "Rebecca Johnson" Subject: [Histonet] Prisma Stainer/coverslipper To: "histonet" Message-ID: <013d01c86092$341481b0$7b48f9d8@CHURCH> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Is anyone using the Prisma coverslipper in Cytology for the Imager? If so how are you doing it? Length of film and level. Thanks Becky ------------------------------ Message: 8 Date: Fri, 27 Jun 2008 09:32:59 -0400 From: Jennifer Johnson Subject: [Histonet] Vacuum pumps To: Message-ID: Content-Type: text/plain; charset="Windows-1252" The James Watson Company sells new & used vacuum pumps. Their phone number is (770) 422-1154. I do not know if they have a web site. They are a direct source instead of having to buy from a medical supply company that will mark up your product. Jennifer Johnson, HTL (ASCP) P.S. I have an affiliation with this company. It is owned by my ex brother-in-law twice removed. _________________________________________________________________ The other season of giving begins 6/24/08. Check out the i???? m Talkathon. http://www.imtalkathon.com?source=TXT_EML_WLH_SeasonOfGiving ------------------------------ Message: 9 Date: Fri, 27 Jun 2008 09:42:28 -0400 From: "Connolly, Brett M" Subject: [Histonet] RE: IHC marker for M-phase cells To: , Message-ID: <63EA0607835FBA4689CEA9EA8B48269201306E3A@usctmx1141.merck.com> Content-Type: text/plain; charset="us-ascii" Thanks to all who replied to my question. Seems like everyone uses anti-phosph-histone H3 (Ser10) as I have also used. However, the Ser10 antibody has also been shown to label interphase G2 cells (Goto et al., J Biol Chem 1999, 274: 25543-25549 and others) and I was really looking for an M-phase only marker. So if anyone has used the phospho-Ser28 histone H3 antibody I would be interested to hear your experiences. Brett > _____________________________________________ > From: Connolly, Brett M > Sent: Thursday, June 26, 2008 9:28 AM > To: histonet@lists.utsouthwestern.edu; ihcrg@googlegroups.com > Subject: IHC marker for M-phase cells > > I am looking for an antibody for labeling FFPE sections of mitotic > cells that are only in M-phase. Found one article in J Histo Cyto > using clone HTA28-phospho-Histone H3(Ser 28) that looked pretty good, > but the Authors produced their own antibody. > > The commercial suppliers I have been able to locate do not indicate > IHC as a suitable application for their products. > > Anyone had experience with this?? > > Or can you recommend another M-phase specific antibody? > > Many thanks, > Brett > > Brett M. Connolly, Ph.D. > Research Fellow, Imaging Research > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > PH 215-652-2501 fax. 215-993-6803 > e-mail. brett_connolly@merck.com > Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 10 Date: Fri, 27 Jun 2008 10:17:17 -0600 From: "Patsy Ruegg" Subject: [Histonet] lab tek metal slide cabinets To: "'Histonet'" Message-ID: <005601c8d871$45f86570$6401a8c0@Patsyoffice> Content-Type: text/plain; charset="us-ascii" I have some brand new lab tek metal slide cabinets that are missing the drawer dividers, does anyone know where I can buy these? They turn the drawer into two so you can line up slides or blocks side by side, without these nothing fits in the drawer. Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 55, Issue 40 **************************************** ------------------------------ Message: 2 Date: Fri, 27 Jun 2008 12:10:48 -0700 From: "R C" Subject: [Histonet] IHC Marker for differentiation of Human to Mouse hepatocytes To: histonet@lists.utsouthwestern.edu Message-ID: <2a926e3f0806271210i5c91c94w131a39870892e9f8@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Is there a known Ab that exhibits + expression of hepatacytes in humans but not mice? Thank you. Ruben Carter ------------------------------ Message: 3 Date: Fri, 27 Jun 2008 15:50:49 -0700 From: "Erin Curry" Subject: [Histonet] FlexStation 3 needs new home To: Message-ID: <005c01c8d8a8$3f42d8f0$bdc88ad0$@com> Content-Type: text/plain; charset="us-ascii" Hello all, We are looking for a new home for our Molecular Devices FlexStation 3 multimode microplate reader. It was new in 2006 and has liquid transfer capabilities. If anyone is interested please contact me at the email address below. Erin Curry 1460 O'Brien Drive Menlo Park, CA 94025 (650) 324-2569 erin@aaisolutions.com ------------------------------ Message: 4 Date: Sat, 28 Jun 2008 00:16:26 +0000 From: JimR0712@comcast.net Subject: [Histonet] MOHS job openings at Loyola University Medical Center To: histonet@lists.utsouthwestern.edu (Histonet2), histonet-request@lists.utsouthwestern.edu (Histonet) Message-ID: <062820080016.19416.486582DA0001C92200004BD82216554886CDCEC9CFAD0307B6@comcast.net> Loyola University Medical has 2 immediate opening for MOHS technicians. These openings are the result of opening a second MOHS facility in LaGrange, Illinois. For full details or to apply online, please go to www.luhs.org/depts/hr or contact Joyce Milewski , HR Manager,at 708-216-9409. Jim Robinson, M.S., HTL/HT (ASCP) Manager, Anatomic Pathology ------------------------------ Message: 5 Date: Fri, 27 Jun 2008 20:43:45 -0400 From: "Jerry Helisek" Subject: [Histonet] RE: slide cabinets, RA Lamb To: , Message-ID: <3855F92002259948A66A8CA2D16E3A4F0B5624@server.ralambusa.com> Content-Type: text/plain; charset="us-ascii" Patsy, RA Lamb has drawer dividers that would work if you do not have any luck finding those. Thanks and have a great weekend. ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ ------------------------------ Message: 10 Date: Fri, 27 Jun 2008 10:17:17 -0600 From: "Patsy Ruegg" Subject: [Histonet] lab tek metal slide cabinets To: "'Histonet'" Message-ID: <005601c8d871$45f86570$6401a8c0@Patsyoffice> Content-Type: text/plain; charset="us-ascii" I have some brand new lab tek metal slide cabinets that are missing the drawer dividers, does anyone know where I can buy these? They turn the drawer into two so you can line up slides or blocks side by side, without these nothing fits in the drawer. Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 55, Issue 40 **************************************** ------------------------------ Message: 6 Date: Fri, 27 Jun 2008 17:46:09 -0700 From: "Fidgen, Laura L." Subject: [Histonet] HT Positions available To: Message-ID: <6BB8BC4519AAB844B174FC739A679BBC0148C025@IRMEXCH01.irm.inhs.org> Content-Type: text/plain; charset="iso-8859-1" Hello Histonetters! Here at Sacred Heart Medical Center in beautiful Spokane, WA we have 3 F/T HT positions available. We have 2 day shifts, 05:00-13:30 and 07:00-15:30, and one evening shift, 15:00-23:30. We offer great wages, benefits, and relocation assistance is available. We have state of the art equipment in a newly constructed laboratory coupled with excellent pathologists, pathologist assistants, and laboratory assistants. For more information please visit the hospital website at www.shmc.org. If interested in any of these positions please contact Debbie Ozust, Employment Specialist, at 509-474-3191. Laura Fidgen, HT(ASCP)cm Histology Supervisor Sacred Heart Medical Center 509-474-4106 ------------------------------ Message: 7 Date: Sat, 28 Jun 2008 11:17:24 +0800 From: "Ryan Salazar" Subject: [Histonet] IHC/ISH To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Deareast Histopeeps! We're currently upgrading our clinical research facility into the world of IHC and ISH. Can you provide us ANY BASIC/ FULL EQUIPMENTS/ PROTOCOLS needed for us to be functional in these procedures? We're currently running the routine H&E and some special stains and we have the instruments needed for these. We just want to be ready for our first IHC and ISH stuffs. Thank you for your humble help and your big contribution! Ryan S. Important : This message is intended for the recipient(s) addressed above. It may contain privileged and confidential information. If you are not the intended recipient, please delete it from your system. You must not copy, use, disseminate, disclose, retain or reproduce all or any part of the information or any attachments from this communication in any form. Thank you. ------------------------------ Message: 8 Date: Sat, 28 Jun 2008 08:38:52 -0700 (PDT) From: Barbara Albert Subject: [Histonet] slide holders for Lerner Linear Stainer To: histonet Message-ID: <994234.4916.qm@web63707.mail.re1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi all, Some time ago someone on this list asked if anyone had slide holders for the old Lerner Linerar stainer.? Well, we just moved and a box turned up.? Let me know if you need them and these brand new slide holders are yours. ? Barbara Albert Lead Histotechnologist UCSF Medical Center San Francisco 415-353-1610 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 55, Issue 41 **************************************** 2007 Best Places to Work Finalist Visit our careers page at www.kaleidahealth.org/careers CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. ------------------------------ Message: 3 Date: Mon, 30 Jun 2008 05:05:43 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Quality assurance To: histonet@lists.utsouthwestern.edu, "Featherstone, Annette" Message-ID: <860432.64776.qm@web65710.mail.ac4.yahoo.com> Content-Type: text/plain; charset=windows-1252 We also used to record when the FS and permanent diagnosis did not completely match, and when the size of the permanent was of only about 50% of the FS section size. Ren? J. --- On Mon, 6/30/08, Featherstone, Annette wrote: From: Featherstone, Annette Subject: [Histonet] Quality assurance To: histonet@lists.utsouthwestern.edu Date: Monday, June 30, 2008, 7:59 AM We collect data from our preclinical (accessioning, registration) and document all registration errors, specimen errors, etc. We collect data from our IHC runs such as controls that do not work, wrong antibodies on tissue, wrong cases cut, tissue falling off the slide etc. We do the same for special stains and H & E's. We document all mislabeled blocks and slides. Annette Featherstone Kaleida Health -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Saturday, June 28, 2008 13:02 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 55, Issue 41 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Quality Assurance Measures (Sarah Kolekamp) 2. IHC Marker for differentiation of Human to Mouse hepatocytes (R C) 3. FlexStation 3 needs new home (Erin Curry) 4. MOHS job openings at Loyola University Medical Center (JimR0712@comcast.net) 5. RE: slide cabinets, RA Lamb (Jerry Helisek) 6. HT Positions available (Fidgen, Laura L.) 7. IHC/ISH (Ryan Salazar) 8. slide holders for Lerner Linear Stainer (Barbara Albert) ---------------------------------------------------------------------- Message: 1 Date: Fri, 27 Jun 2008 13:17:33 -0400 From: "Sarah Kolekamp" Subject: [Histonet] Quality Assurance Measures To: Message-ID: <4864E86D0200006100015432@nodcgwcluster_gw4_server.no.trinity-health.org> Content-Type: text/plain; charset=UTF-8 Is anyone currently running a quality assurance program in their histology lab, if so what do you measure/how? We are tring to develop some standards and would like to have data to run analysis on...any ideas on what data to collect?? Thanks, Sarah Kolekamp Sarah Kolekamp HTL (ASCP) Lead Histotechnologist Saint Mary's Health Care Phone Number (616) 752-6155 Fax Number (616) 774-0628 >>> 06/27/08 1:02 PM >>> Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. IHC marker for M-phase cells (Jean-Martin Lapointe) 2. HMB45 antibody (MaryAnn Dixon) 3. Formalin Recyling (John Spair) 4. Re: HMB45 antibody (Jan Shivers) 5. RE: whole mount cryosections (Atoska Gentry) 6. whole mount cryosections (Stephen Peters M.D.) 7. Prisma Stainer/coverslipper (Rebecca Johnson) 8. Vacuum pumps (Jennifer Johnson) 9. RE: IHC marker for M-phase cells (Connolly, Brett M) 10. lab tek metal slide cabinets (Patsy Ruegg) ---------------------------------------------------------------------- Message: 1 Date: Thu, 26 Jun 2008 13:23:17 -0400 From: "Jean-Martin Lapointe" Subject: [Histonet] IHC marker for M-phase cells To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Brett, at my previous job we did IHC staining for phospho-Histone H3 (ser10) using the Cell Signaling rabbit monoclonal antibody CS9701 in FFPE tissue, and it worked beautifully. It was on human origin tissue, don't remember cross-reactivity in other species but Cell Signaling mentions it reacts with many others. Good luck Jean-Martin __________________________________ Jean-Martin Lapointe, DMV, MS, dACVP Vice-President, Pathologie AccelLAB Inc 1635 Lionel-Bertrand, Boisbriand Qu??bec, Canada J7H 1N8 tel:?? 450-435-9482 ext.247 fax: 450-435-4795 jm.lapointe@accellab.com ?? ?? ________________________________________________________________ Message: 6 Date: Thu, 26 Jun 2008 09:27:58 -0400 From: "Connolly, Brett M" Subject: [Histonet] IHC marker for M-phase cells To: , Message-ID: <63EA0607835FBA4689CEA9EA8B48269201306BD2@usctmx1141.merck.com> Content-Type: text/plain; charset="us-ascii" I am looking for an antibody for labeling FFPE sections of mitotic cells that are only in M-phase. Found one article in J Histo Cyto using clone HTA28-phospho-Histone H3(Ser 28) that looked pretty good, but the Authors produced their own antibody. The commercial suppliers I have been able to locate do not indicate IHC as a suitable application for their products. Anyone had experience with this?? Or can you recommend another M-phase specific antibody? Many thanks, Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Research Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com ******************** ------------------------------ Message: 2 Date: Thu, 26 Jun 2008 13:56:05 -0400 From: "MaryAnn Dixon" Subject: [Histonet] HMB45 antibody To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi histonetters, Anyone out there successfully using a monoclonal HMB45 antibody on canine tissues? I think I've read a dozen papers all with different protocols and my eyes are about to pop out of my head! Currently I'm at a 1:50 dilution with a pretreatment in a citrate buffer (pressure cooker). I have my choice of a polymer based detection as well as a biotinylated detection. Any help would be appreciated. Thanks in advance. MaryAnn Dixon BS Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 392-2235 Ext. 4517 ------------------------------ Message: 3 Date: Thu, 26 Jun 2008 10:53:23 -0700 From: "John Spair" Subject: [Histonet] Formalin Recyling To: histonet@lists.utsouthwestern.edu Message-ID: <61A9977919846C479389493BAE2517CA023760EC@MHSEXMBX1.multicare.org> Content-Type: text/plain; charset=us-ascii Is anyone using the formalin recycling/filtration system sold by Creative Waste Solutions?? If you are , please email me at: john.spair@multicare.org if you don't mind, I have some questions. Thank you. "MMS " made the following annotations. ------------------------------------------------------------------------------ NOTICE: This e-mail and the attachments hereto, if any, may contain privileged and/or confidential information. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, you are hereby notified that any examination, distribution or copying of this e-mail and the attachments hereto, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by email or telephone and permanently delete this e-mail and the attachments hereto, if any, and destroy any printout thereof. MultiCare Health System, Tacoma, WA 98415 (253) 403-1000. ============================================================================== ------------------------------ Message: 4 Date: Thu, 26 Jun 2008 15:10:29 -0500 From: "Jan Shivers" Subject: Re: [Histonet] HMB45 antibody To: "MaryAnn Dixon" , Message-ID: Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original I had little or no success with HMB45 on canine tissue. I use Melan-A exclusively; that works fine. Jan Shivers Senior Scientist Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu ----- Original Message ----- From: "MaryAnn Dixon" To: Sent: Thursday, June 26, 2008 12:56 PM Subject: [Histonet] HMB45 antibody Hi histonetters, Anyone out there successfully using a monoclonal HMB45 antibody on canine tissues? I think I've read a dozen papers all with different protocols and my eyes are about to pop out of my head! Currently I'm at a 1:50 dilution with a pretreatment in a citrate buffer (pressure cooker). I have my choice of a polymer based detection as well as a biotinylated detection. Any help would be appreciated. Thanks in advance. MaryAnn Dixon BS Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 392-2235 Ext. 4517 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Thu, 26 Jun 2008 16:52:01 -0500 From: Atoska Gentry Subject: [Histonet] RE: whole mount cryosections To: Histonet Message-ID: <48640F81.7080301@vetmed.auburn.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed hello, will someone with expertise in fresh frozen whole mount and/or coronal primate brain sections please contact me with pointers on requirements for this type sMicrom HM 505 E that will accommodate such samples? We normally section standard size sections but now one of our PI's is considering trying larger samples. Thanks, Atoska ------------------------------ Message: 6 Date: Thu, 26 Jun 2008 15:52:50 -0700 (PDT) From: "Stephen Peters M.D." Subject: [Histonet] whole mount cryosections To: Histonet@lists.utsouthwestern.edu Message-ID: <567183.62199.qm@web45114.mail.sp1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Atoska, ?? Our 50 x 30 mm well bar set?? with 55 x 35 mm chucks may give a bit more room to work. See item # 115 on this page: http://pathologyinnovations.com/Price%20List.htm ?? Stephen ?? ------------------------------ Message: 7 Date: Sat, 26 Jan 2008 22:10:43 -0500 From: "Rebecca Johnson" Subject: [Histonet] Prisma Stainer/coverslipper To: "histonet" Message-ID: <013d01c86092$341481b0$7b48f9d8@CHURCH> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Is anyone using the Prisma coverslipper in Cytology for the Imager? If so how are you doing it? Length of film and level. Thanks Becky ------------------------------ Message: 8 Date: Fri, 27 Jun 2008 09:32:59 -0400 From: Jennifer Johnson Subject: [Histonet] Vacuum pumps To: Message-ID: Content-Type: text/plain; charset="Windows-1252" The James Watson Company sells new & used vacuum pumps. Their phone number is (770) 422-1154. I do not know if they have a web site. They are a direct source instead of having to buy from a medical supply company that will mark up your product. Jennifer Johnson, HTL (ASCP) P.S. I have an affiliation with this company. It is owned by my ex brother-in-law twice removed. _________________________________________________________________ The other season of giving begins 6/24/08. Check out the i??m Talkathon. http://www.imtalkathon.com?source=TXT_EML_WLH_SeasonOfGiving ------------------------------ Message: 9 Date: Fri, 27 Jun 2008 09:42:28 -0400 From: "Connolly, Brett M" Subject: [Histonet] RE: IHC marker for M-phase cells To: , Message-ID: <63EA0607835FBA4689CEA9EA8B48269201306E3A@usctmx1141.merck.com> Content-Type: text/plain; charset="us-ascii" Thanks to all who replied to my question. Seems like everyone uses anti-phosph-histone H3 (Ser10) as I have also used. However, the Ser10 antibody has also been shown to label interphase G2 cells (Goto et al., J Biol Chem 1999, 274: 25543-25549 and others) and I was really looking for an M-phase only marker. So if anyone has used the phospho-Ser28 histone H3 antibody I would be interested to hear your experiences. Brett > _____________________________________________ > From: Connolly, Brett M > Sent: Thursday, June 26, 2008 9:28 AM > To: histonet@lists.utsouthwestern.edu; ihcrg@googlegroups.com > Subject: IHC marker for M-phase cells > > I am looking for an antibody for labeling FFPE sections of mitotic > cells that are only in M-phase. Found one article in J Histo Cyto > using clone HTA28-phospho-Histone H3(Ser 28) that looked pretty good, > but the Authors produced their own antibody. > > The commercial suppliers I have been able to locate do not indicate > IHC as a suitable application for their products. > > Anyone had experience with this?? > > Or can you recommend another M-phase specific antibody? > > Many thanks, > Brett > > Brett M. Connolly, Ph.D. > Research Fellow, Imaging Research > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > PH 215-652-2501 fax. 215-993-6803 > e-mail. brett_connolly@merck.com > Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 10 Date: Fri, 27 Jun 2008 10:17:17 -0600 From: "Patsy Ruegg" Subject: [Histonet] lab tek metal slide cabinets To: "'Histonet'" Message-ID: <005601c8d871$45f86570$6401a8c0@Patsyoffice> Content-Type: text/plain; charset="us-ascii" I have some brand new lab tek metal slide cabinets that are missing the drawer dividers, does anyone know where I can buy these? They turn the drawer into two so you can line up slides or blocks side by side, without these nothing fits in the drawer. Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 55, Issue 40 **************************************** ------------------------------ Message: 2 Date: Fri, 27 Jun 2008 12:10:48 -0700 From: "R C" Subject: [Histonet] IHC Marker for differentiation of Human to Mouse hepatocytes To: histonet@lists.utsouthwestern.edu Message-ID: <2a926e3f0806271210i5c91c94w131a39870892e9f8@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Is there a known Ab that exhibits + expression of hepatacytes in humans but not mice? Thank you. Ruben Carter ------------------------------ Message: 3 Date: Fri, 27 Jun 2008 15:50:49 -0700 From: "Erin Curry" Subject: [Histonet] FlexStation 3 needs new home To: Message-ID: <005c01c8d8a8$3f42d8f0$bdc88ad0$@com> Content-Type: text/plain; charset="us-ascii" Hello all, We are looking for a new home for our Molecular Devices FlexStation 3 multimode microplate reader. It was new in 2006 and has liquid transfer capabilities. If anyone is interested please contact me at the email address below. Erin Curry 1460 O'Brien Drive Menlo Park, CA 94025 (650) 324-2569 erin@aaisolutions.com ------------------------------ Message: 4 Date: Sat, 28 Jun 2008 00:16:26 +0000 From: JimR0712@comcast.net Subject: [Histonet] MOHS job openings at Loyola University Medical Center To: histonet@lists.utsouthwestern.edu (Histonet2), histonet-request@lists.utsouthwestern.edu (Histonet) Message-ID: <062820080016.19416.486582DA0001C92200004BD82216554886CDCEC9CFAD0307B6@comcast.net> Loyola University Medical has 2 immediate opening for MOHS technicians. These openings are the result of opening a second MOHS facility in LaGrange, Illinois. For full details or to apply online, please go to www.luhs.org/depts/hr or contact Joyce Milewski , HR Manager,at 708-216-9409. Jim Robinson, M.S., HTL/HT (ASCP) Manager, Anatomic Pathology ------------------------------ Message: 5 Date: Fri, 27 Jun 2008 20:43:45 -0400 From: "Jerry Helisek" Subject: [Histonet] RE: slide cabinets, RA Lamb To: , Message-ID: <3855F92002259948A66A8CA2D16E3A4F0B5624@server.ralambusa.com> Content-Type: text/plain; charset="us-ascii" Patsy, RA Lamb has drawer dividers that would work if you do not have any luck finding those. Thanks and have a great weekend. ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ ------------------------------ Message: 10 Date: Fri, 27 Jun 2008 10:17:17 -0600 From: "Patsy Ruegg" Subject: [Histonet] lab tek metal slide cabinets To: "'Histonet'" Message-ID: <005601c8d871$45f86570$6401a8c0@Patsyoffice> Content-Type: text/plain; charset="us-ascii" I have some brand new lab tek metal slide cabinets that are missing the drawer dividers, does anyone know where I can buy these? They turn the drawer into two so you can line up slides or blocks side by side, without these nothing fits in the drawer. Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 55, Issue 40 **************************************** ------------------------------ Message: 6 Date: Fri, 27 Jun 2008 17:46:09 -0700 From: "Fidgen, Laura L." Subject: [Histonet] HT Positions available To: Message-ID: <6BB8BC4519AAB844B174FC739A679BBC0148C025@IRMEXCH01.irm.inhs.org> Content-Type: text/plain; charset="iso-8859-1" Hello Histonetters! Here at Sacred Heart Medical Center in beautiful Spokane, WA we have 3 F/T HT positions available. We have 2 day shifts, 05:00-13:30 and 07:00-15:30, and one evening shift, 15:00-23:30. We offer great wages, benefits, and relocation assistance is available. We have state of the art equipment in a newly constructed laboratory coupled with excellent pathologists, pathologist assistants, and laboratory assistants. For more information please visit the hospital website at www.shmc.org. If interested in any of these positions please contact Debbie Ozust, Employment Specialist, at 509-474-3191. Laura Fidgen, HT(ASCP)cm Histology Supervisor Sacred Heart Medical Center 509-474-4106 ------------------------------ Message: 7 Date: Sat, 28 Jun 2008 11:17:24 +0800 From: "Ryan Salazar" Subject: [Histonet] IHC/ISH To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Deareast Histopeeps! We're currently upgrading our clinical research facility into the world of IHC and ISH. Can you provide us ANY BASIC/ FULL EQUIPMENTS/ PROTOCOLS needed for us to be functional in these procedures? We're currently running the routine H&E and some special stains and we have the instruments needed for these. We just want to be ready for our first IHC and ISH stuffs. Thank you for your humble help and your big contribution! Ryan S. Important : This message is intended for the recipient(s) addressed above. It may contain privileged and confidential information. If you are not the intended recipient, please delete it from your system. You must not copy, use, disseminate, disclose, retain or reproduce all or any part of the information or any attachments from this communication in any form. Thank you. ------------------------------ Message: 8 Date: Sat, 28 Jun 2008 08:38:52 -0700 (PDT) From: Barbara Albert Subject: [Histonet] slide holders for Lerner Linear Stainer To: histonet Message-ID: <994234.4916.qm@web63707.mail.re1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi all, Some time ago someone on this list asked if anyone had slide holders for the old Lerner Linerar stainer. Well, we just moved and a box turned up. Let me know if you need them and these brand new slide holders are yours. Barbara Albert Lead Histotechnologist UCSF Medical Center San Francisco 415-353-1610 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 55, Issue 41 **************************************** 2007 Best Places to Work Finalist Visit our careers page at www.kaleidahealth.org/careers CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Mon, 30 Jun 2008 19:04:16 +0530 (IST) From: abi jag Subject: [Histonet] staining manual reg To: histonet@lists.utsouthwestern.edu Message-ID: <469154.7181.qm@web95106.mail.in2.yahoo.com> Content-Type: text/plain; charset=utf-8 Dear Histonetters, I am working as a? histologist in one of the pharmaceutical companies in India. I have accepted an opportunity to set up a histology lab(mainly rodent tissues). I have an query. ? In our archives, i have found some references about Richard Allan website staining manual which is having very useful information for trouble shooting. ? But i didn't find any staining manual and also? Richard Allan is now a part of thermo. ? If any of our fellow histonetters have any idea about this staining manual or its contents. ? thanks for all your help ? ABI ? ? ? ? Explore your hobbies and interests. Go to http://in.promos.yahoo.com/groups/ ------------------------------ Message: 5 Date: Mon, 30 Jun 2008 07:20:23 -0700 From: "Michael Mihalik" Subject: RE: [Histonet] slide printers To: "'Jeanne Estabel'" , Message-ID: <6D3D5A3D481A49ABA9A78EAAD1DED567@MDMM1330> Content-Type: text/plain; charset="US-ASCII" Good morning, You may want to check the archives as there have been a few postings on this subject recently. Depending on your usage, you may want to consider slide LABEL printers rather than slide etchers. In our application, we need the label/slide to be generated just prior to the histotechnologist cutting the tissue. Because this requires a slide printer/labeler to be next to each microtome and because we need these slides/labels to print very quickly, we chose to go with a label printer rather than an etcher. If you would like some details on the printer or would like to hear further details, please let me know. Michael Mihalik PathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeanne Estabel Sent: Monday, June 30, 2008 4:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slide printers Hi, Can I have some feedback on slide printers? Regards Jeanne Jeanne Estabel, PhD MGP Histologist, Team 109 Wellcome Trust/Sanger Institute Hinxton CB10 1SA Tel: 01223 495338 -- The Wellcome Trust Sanger Institute is operated by Genome Research Limited, a charity registered in England with number 1021457 and a compa ny registered in England with number 2742969, whose registered office is 2 15 Euston Road, London, NW1 2BE. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Mon, 30 Jun 2008 10:56:03 -0500 From: "Hector Hernandez" Subject: [Histonet] HT Positions available To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hello Histonetters! Currently PRL (Pathology Reference Lab) is looking for 3 Histology positions to fill. Two will be day shift positions and one will be an evening position 5PM-1:30AM at a satellite lab. We offer excellent benefits and wages will depend on your experience. We are located in warm-hot San Antonio Texas. If interested in any of these positions contact Hector Hernandez at (210)892-3747. Hector Hernandez, HT (ASCP) Histology Supervisor 210-892-3747 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 55, Issue 42 **************************************** This communication is intended for the use of the recipient to which it is addressed, and may contain confidential, personal and or privileged information. Please contact us immediately if you are not the intended recipient. Do not copy, distribute or take action relying on it. Any communication received in error, or subsequent reply, should be deleted or destroyed. From freckles9660 <@t> yahoo.com Mon Jun 30 12:19:45 2008 From: freckles9660 <@t> yahoo.com (Karla Arrington) Date: Mon Jun 30 12:19:48 2008 Subject: [Histonet] Speed Message-ID: <635818.32288.qm@web32505.mail.mud.yahoo.com> Histo's: ?Generally speaking, what is the routine number of?tissues (cassettes)?a tech should embed in an hour? Also how many blocks should a tech be able to cut in an hour? Thanks, Karla Arrington From mike <@t> pathview.com Mon Jun 30 12:33:45 2008 From: mike <@t> pathview.com (Michael Mihalik) Date: Mon Jun 30 12:34:25 2008 Subject: [Histonet] HISTOLOGY QUALITY ASSURANCE RE: Histonet Digest, Vol 55, Issue 42 In-Reply-To: <9C0BD812BAB0BA4DB7E7FD4F5FA3CAA80A494BA3@exbe.chr.ab.ca> References: <9C0BD812BAB0BA4DB7E7FD4F5FA3CAA80A494BA3@exbe.chr.ab.ca> Message-ID: <90B1A48D878440DB981BE65CE394EFA0@MDMM1330> I'm curious. How do you guys 'document' this? By this question, I?m asking how do you record it?.... paper log, excel spreadsheet, what? Michael Mihalik PathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kay, Karen Sent: Monday, June 30, 2008 10:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HISTOLOGY QUALITY ASSURANCE RE: Histonet Digest,Vol 55, Issue 42 Hi Sarah, We document control results for all special stains, IHC, and routine H&E. In addition we also track all occurrences, i.e. milabelled slides, blocks, specimen accessioning issues, tissue pick-up on wrong slides, tissue floaters, dictation issues and any injuries. Also number of days within a defined period where work is not completed. We also run and track and extensive QA program from the pathologist's perspective with parameters set as indicated by the consulting pathologist for the area. Karen Kay Chinook Health Region Laboratory Lethbridge, Alberta, CANADA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: June 30, 2008 11:09 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 55, Issue 42 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. slide printers (Jeanne Estabel) 2. Quality assurance (Featherstone, Annette) 3. Re: Quality assurance (Rene J Buesa) 4. staining manual reg (abi jag) 5. RE: slide printers (Michael Mihalik) 6. HT Positions available (Hector Hernandez) ---------------------------------------------------------------------- Message: 1 Date: Mon, 30 Jun 2008 12:02:57 +0100 From: "Jeanne Estabel" Subject: [Histonet] slide printers To: Message-ID: <0526C4B4E593154B86E6A8651913C28D0245360E@exchsrv2.internal.sanger.ac.uk> Content-Type: text/plain; charset="us-ascii" Hi, Can I have some feedback on slide printers? Regards Jeanne Jeanne Estabel, PhD MGP Histologist, Team 109 Wellcome Trust/Sanger Institute Hinxton CB10 1SA Tel: 01223 495338 -- The Wellcome Trust Sanger Institute is operated by Genome Research Limited, a charity registered in England with number 1021457 and a compa ny registered in England with number 2742969, whose registered office is 2 15 Euston Road, London, NW1 2BE. ------------------------------ Message: 2 Date: Mon, 30 Jun 2008 07:59:22 -0400 From: "Featherstone, Annette" Subject: [Histonet] Quality assurance To: Message-ID: <16A5E67B2A1F714885DEDC2CB68DD6091D79F7@KALEXMB03.KaleidaHealth.org> Content-Type: text/plain; charset="utf-8" We collect data from our preclinical (accessioning, registration) and document all registration errors, specimen errors, etc. We collect data from our IHC runs such as controls that do not work, wrong antibodies on tissue, wrong cases cut, tissue falling off the slide etc. We do the same for special stains and H & E's. We document all mislabeled blocks and slides. Annette Featherstone Kaleida Health -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Saturday, June 28, 2008 13:02 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 55, Issue 41 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Quality Assurance Measures (Sarah Kolekamp) 2. IHC Marker for differentiation of Human to Mouse hepatocytes (R C) 3. FlexStation 3 needs new home (Erin Curry) 4. MOHS job openings at Loyola University Medical Center (JimR0712@comcast.net) 5. RE: slide cabinets, RA Lamb (Jerry Helisek) 6. HT Positions available (Fidgen, Laura L.) 7. IHC/ISH (Ryan Salazar) 8. slide holders for Lerner Linear Stainer (Barbara Albert) ---------------------------------------------------------------------- Message: 1 Date: Fri, 27 Jun 2008 13:17:33 -0400 From: "Sarah Kolekamp" Subject: [Histonet] Quality Assurance Measures To: Message-ID: <4864E86D0200006100015432@nodcgwcluster_gw4_server.no.trinity-health.org> Content-Type: text/plain; charset=UTF-8 Is anyone currently running a quality assurance program in their histology lab, if so what do you measure/how? We are tring to develop some standards and would like to have data to run analysis on...any ideas on what data to collect?? Thanks, Sarah Kolekamp Sarah Kolekamp HTL (ASCP) Lead Histotechnologist Saint Mary's Health Care Phone Number (616) 752-6155 Fax Number (616) 774-0628 >>> 06/27/08 1:02 PM >>> Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. IHC marker for M-phase cells (Jean-Martin Lapointe) 2. HMB45 antibody (MaryAnn Dixon) 3. Formalin Recyling (John Spair) 4. Re: HMB45 antibody (Jan Shivers) 5. RE: whole mount cryosections (Atoska Gentry) 6. whole mount cryosections (Stephen Peters M.D.) 7. Prisma Stainer/coverslipper (Rebecca Johnson) 8. Vacuum pumps (Jennifer Johnson) 9. RE: IHC marker for M-phase cells (Connolly, Brett M) 10. lab tek metal slide cabinets (Patsy Ruegg) ---------------------------------------------------------------------- Message: 1 Date: Thu, 26 Jun 2008 13:23:17 -0400 From: "Jean-Martin Lapointe" Subject: [Histonet] IHC marker for M-phase cells To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Brett, at my previous job we did IHC staining for phospho-Histone H3 (ser10) using the Cell Signaling rabbit monoclonal antibody CS9701 in FFPE tissue, and it worked beautifully. It was on human origin tissue, don't remember cross-reactivity in other species but Cell Signaling mentions it reacts with many others. Good luck Jean-Martin __________________________________ Jean-Martin Lapointe, DMV, MS, dACVP Vice-President, Pathologie AccelLAB Inc 1635 Lionel-Bertrand, Boisbriand Qu????bec, Canada J7H 1N8 tel:???? 450-435-9482 ext.247 fax: 450-435-4795 jm.lapointe@accellab.com ???? ???? ________________________________________________________________ Message: 6 Date: Thu, 26 Jun 2008 09:27:58 -0400 From: "Connolly, Brett M" Subject: [Histonet] IHC marker for M-phase cells To: , Message-ID: <63EA0607835FBA4689CEA9EA8B48269201306BD2@usctmx1141.merck.com> Content-Type: text/plain; charset="us-ascii" I am looking for an antibody for labeling FFPE sections of mitotic cells that are only in M-phase. Found one article in J Histo Cyto using clone HTA28-phospho-Histone H3(Ser 28) that looked pretty good, but the Authors produced their own antibody. The commercial suppliers I have been able to locate do not indicate IHC as a suitable application for their products. Anyone had experience with this?? Or can you recommend another M-phase specific antibody? Many thanks, Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Research Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com ******************** ------------------------------ Message: 2 Date: Thu, 26 Jun 2008 13:56:05 -0400 From: "MaryAnn Dixon" Subject: [Histonet] HMB45 antibody To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi histonetters, Anyone out there successfully using a monoclonal HMB45 antibody on canine tissues? I think I've read a dozen papers all with different protocols and my eyes are about to pop out of my head! Currently I'm at a 1:50 dilution with a pretreatment in a citrate buffer (pressure cooker). I have my choice of a polymer based detection as well as a biotinylated detection. Any help would be appreciated. Thanks in advance. MaryAnn Dixon BS Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 392-2235 Ext. 4517 ------------------------------ Message: 3 Date: Thu, 26 Jun 2008 10:53:23 -0700 From: "John Spair" Subject: [Histonet] Formalin Recyling To: histonet@lists.utsouthwestern.edu Message-ID: <61A9977919846C479389493BAE2517CA023760EC@MHSEXMBX1.multicare.org> Content-Type: text/plain; charset=us-ascii Is anyone using the formalin recycling/filtration system sold by Creative Waste Solutions?? If you are , please email me at: john.spair@multicare.org if you don't mind, I have some questions. Thank you. "MMS " made the following annotations. ------------------------------------------------------------------------------ NOTICE: This e-mail and the attachments hereto, if any, may contain privileged and/or confidential information. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, you are hereby notified that any examination, distribution or copying of this e-mail and the attachments hereto, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by email or telephone and permanently delete this e-mail and the attachments hereto, if any, and destroy any printout thereof. MultiCare Health System, Tacoma, WA 98415 (253) 403-1000. ============================================================================== ------------------------------ Message: 4 Date: Thu, 26 Jun 2008 15:10:29 -0500 From: "Jan Shivers" Subject: Re: [Histonet] HMB45 antibody To: "MaryAnn Dixon" , Message-ID: Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original I had little or no success with HMB45 on canine tissue. I use Melan-A exclusively; that works fine. Jan Shivers Senior Scientist Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu ----- Original Message ----- From: "MaryAnn Dixon" To: Sent: Thursday, June 26, 2008 12:56 PM Subject: [Histonet] HMB45 antibody Hi histonetters, Anyone out there successfully using a monoclonal HMB45 antibody on canine tissues? I think I've read a dozen papers all with different protocols and my eyes are about to pop out of my head! Currently I'm at a 1:50 dilution with a pretreatment in a citrate buffer (pressure cooker). I have my choice of a polymer based detection as well as a biotinylated detection. Any help would be appreciated. Thanks in advance. MaryAnn Dixon BS Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 392-2235 Ext. 4517 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Thu, 26 Jun 2008 16:52:01 -0500 From: Atoska Gentry Subject: [Histonet] RE: whole mount cryosections To: Histonet Message-ID: <48640F81.7080301@vetmed.auburn.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed hello, will someone with expertise in fresh frozen whole mount and/or coronal primate brain sections please contact me with pointers on requirements for this type sMicrom HM 505 E that will accommodate such samples? We normally section standard size sections but now one of our PI's is considering trying larger samples. Thanks, Atoska ------------------------------ Message: 6 Date: Thu, 26 Jun 2008 15:52:50 -0700 (PDT) From: "Stephen Peters M.D." Subject: [Histonet] whole mount cryosections To: Histonet@lists.utsouthwestern.edu Message-ID: <567183.62199.qm@web45114.mail.sp1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Atoska, ???? Our 50 x 30 mm well bar set???? with 55 x 35 mm chucks may give a bit more room to work. See item # 115 on this page: http://pathologyinnovations.com/Price%20List.htm ???? Stephen ???? ------------------------------ Message: 7 Date: Sat, 26 Jan 2008 22:10:43 -0500 From: "Rebecca Johnson" Subject: [Histonet] Prisma Stainer/coverslipper To: "histonet" Message-ID: <013d01c86092$341481b0$7b48f9d8@CHURCH> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Is anyone using the Prisma coverslipper in Cytology for the Imager? If so how are you doing it? Length of film and level. Thanks Becky ------------------------------ Message: 8 Date: Fri, 27 Jun 2008 09:32:59 -0400 From: Jennifer Johnson Subject: [Histonet] Vacuum pumps To: Message-ID: Content-Type: text/plain; charset="Windows-1252" The James Watson Company sells new & used vacuum pumps. Their phone number is (770) 422-1154. I do not know if they have a web site. They are a direct source instead of having to buy from a medical supply company that will mark up your product. Jennifer Johnson, HTL (ASCP) P.S. I have an affiliation with this company. It is owned by my ex brother-in-law twice removed. _________________________________________________________________ The other season of giving begins 6/24/08. Check out the i???? m Talkathon. http://www.imtalkathon.com?source=TXT_EML_WLH_SeasonOfGiving ------------------------------ Message: 9 Date: Fri, 27 Jun 2008 09:42:28 -0400 From: "Connolly, Brett M" Subject: [Histonet] RE: IHC marker for M-phase cells To: , Message-ID: <63EA0607835FBA4689CEA9EA8B48269201306E3A@usctmx1141.merck.com> Content-Type: text/plain; charset="us-ascii" Thanks to all who replied to my question. Seems like everyone uses anti-phosph-histone H3 (Ser10) as I have also used. However, the Ser10 antibody has also been shown to label interphase G2 cells (Goto et al., J Biol Chem 1999, 274: 25543-25549 and others) and I was really looking for an M-phase only marker. So if anyone has used the phospho-Ser28 histone H3 antibody I would be interested to hear your experiences. Brett > _____________________________________________ > From: Connolly, Brett M > Sent: Thursday, June 26, 2008 9:28 AM > To: histonet@lists.utsouthwestern.edu; ihcrg@googlegroups.com > Subject: IHC marker for M-phase cells > > I am looking for an antibody for labeling FFPE sections of mitotic > cells that are only in M-phase. Found one article in J Histo Cyto > using clone HTA28-phospho-Histone H3(Ser 28) that looked pretty good, > but the Authors produced their own antibody. > > The commercial suppliers I have been able to locate do not indicate > IHC as a suitable application for their products. > > Anyone had experience with this?? > > Or can you recommend another M-phase specific antibody? > > Many thanks, > Brett > > Brett M. Connolly, Ph.D. > Research Fellow, Imaging Research > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > PH 215-652-2501 fax. 215-993-6803 > e-mail. brett_connolly@merck.com > Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 10 Date: Fri, 27 Jun 2008 10:17:17 -0600 From: "Patsy Ruegg" Subject: [Histonet] lab tek metal slide cabinets To: "'Histonet'" Message-ID: <005601c8d871$45f86570$6401a8c0@Patsyoffice> Content-Type: text/plain; charset="us-ascii" I have some brand new lab tek metal slide cabinets that are missing the drawer dividers, does anyone know where I can buy these? They turn the drawer into two so you can line up slides or blocks side by side, without these nothing fits in the drawer. Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 55, Issue 40 **************************************** ------------------------------ Message: 2 Date: Fri, 27 Jun 2008 12:10:48 -0700 From: "R C" Subject: [Histonet] IHC Marker for differentiation of Human to Mouse hepatocytes To: histonet@lists.utsouthwestern.edu Message-ID: <2a926e3f0806271210i5c91c94w131a39870892e9f8@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Is there a known Ab that exhibits + expression of hepatacytes in humans but not mice? Thank you. Ruben Carter ------------------------------ Message: 3 Date: Fri, 27 Jun 2008 15:50:49 -0700 From: "Erin Curry" Subject: [Histonet] FlexStation 3 needs new home To: Message-ID: <005c01c8d8a8$3f42d8f0$bdc88ad0$@com> Content-Type: text/plain; charset="us-ascii" Hello all, We are looking for a new home for our Molecular Devices FlexStation 3 multimode microplate reader. It was new in 2006 and has liquid transfer capabilities. If anyone is interested please contact me at the email address below. Erin Curry 1460 O'Brien Drive Menlo Park, CA 94025 (650) 324-2569 erin@aaisolutions.com ------------------------------ Message: 4 Date: Sat, 28 Jun 2008 00:16:26 +0000 From: JimR0712@comcast.net Subject: [Histonet] MOHS job openings at Loyola University Medical Center To: histonet@lists.utsouthwestern.edu (Histonet2), histonet-request@lists.utsouthwestern.edu (Histonet) Message-ID: <062820080016.19416.486582DA0001C92200004BD82216554886CDCEC9CFAD0307B6@comcast.net> Loyola University Medical has 2 immediate opening for MOHS technicians. These openings are the result of opening a second MOHS facility in LaGrange, Illinois. For full details or to apply online, please go to www.luhs.org/depts/hr or contact Joyce Milewski , HR Manager,at 708-216-9409. Jim Robinson, M.S., HTL/HT (ASCP) Manager, Anatomic Pathology ------------------------------ Message: 5 Date: Fri, 27 Jun 2008 20:43:45 -0400 From: "Jerry Helisek" Subject: [Histonet] RE: slide cabinets, RA Lamb To: , Message-ID: <3855F92002259948A66A8CA2D16E3A4F0B5624@server.ralambusa.com> Content-Type: text/plain; charset="us-ascii" Patsy, RA Lamb has drawer dividers that would work if you do not have any luck finding those. Thanks and have a great weekend. ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ ------------------------------ Message: 10 Date: Fri, 27 Jun 2008 10:17:17 -0600 From: "Patsy Ruegg" Subject: [Histonet] lab tek metal slide cabinets To: "'Histonet'" Message-ID: <005601c8d871$45f86570$6401a8c0@Patsyoffice> Content-Type: text/plain; charset="us-ascii" I have some brand new lab tek metal slide cabinets that are missing the drawer dividers, does anyone know where I can buy these? They turn the drawer into two so you can line up slides or blocks side by side, without these nothing fits in the drawer. Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 55, Issue 40 **************************************** ------------------------------ Message: 6 Date: Fri, 27 Jun 2008 17:46:09 -0700 From: "Fidgen, Laura L." Subject: [Histonet] HT Positions available To: Message-ID: <6BB8BC4519AAB844B174FC739A679BBC0148C025@IRMEXCH01.irm.inhs.org> Content-Type: text/plain; charset="iso-8859-1" Hello Histonetters! Here at Sacred Heart Medical Center in beautiful Spokane, WA we have 3 F/T HT positions available. We have 2 day shifts, 05:00-13:30 and 07:00-15:30, and one evening shift, 15:00-23:30. We offer great wages, benefits, and relocation assistance is available. We have state of the art equipment in a newly constructed laboratory coupled with excellent pathologists, pathologist assistants, and laboratory assistants. For more information please visit the hospital website at www.shmc.org. If interested in any of these positions please contact Debbie Ozust, Employment Specialist, at 509-474-3191. Laura Fidgen, HT(ASCP)cm Histology Supervisor Sacred Heart Medical Center 509-474-4106 ------------------------------ Message: 7 Date: Sat, 28 Jun 2008 11:17:24 +0800 From: "Ryan Salazar" Subject: [Histonet] IHC/ISH To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Deareast Histopeeps! We're currently upgrading our clinical research facility into the world of IHC and ISH. Can you provide us ANY BASIC/ FULL EQUIPMENTS/ PROTOCOLS needed for us to be functional in these procedures? We're currently running the routine H&E and some special stains and we have the instruments needed for these. We just want to be ready for our first IHC and ISH stuffs. Thank you for your humble help and your big contribution! Ryan S. Important : This message is intended for the recipient(s) addressed above. It may contain privileged and confidential information. If you are not the intended recipient, please delete it from your system. You must not copy, use, disseminate, disclose, retain or reproduce all or any part of the information or any attachments from this communication in any form. Thank you. ------------------------------ Message: 8 Date: Sat, 28 Jun 2008 08:38:52 -0700 (PDT) From: Barbara Albert Subject: [Histonet] slide holders for Lerner Linear Stainer To: histonet Message-ID: <994234.4916.qm@web63707.mail.re1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi all, Some time ago someone on this list asked if anyone had slide holders for the old Lerner Linerar stainer.? Well, we just moved and a box turned up.? Let me know if you need them and these brand new slide holders are yours. ? Barbara Albert Lead Histotechnologist UCSF Medical Center San Francisco 415-353-1610 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 55, Issue 41 **************************************** 2007 Best Places to Work Finalist Visit our careers page at www.kaleidahealth.org/careers CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. ------------------------------ Message: 3 Date: Mon, 30 Jun 2008 05:05:43 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Quality assurance To: histonet@lists.utsouthwestern.edu, "Featherstone, Annette" Message-ID: <860432.64776.qm@web65710.mail.ac4.yahoo.com> Content-Type: text/plain; charset=windows-1252 We also used to record when the FS and permanent diagnosis did not completely match, and when the size of the permanent was of only about 50% of the FS section size. Ren? J. --- On Mon, 6/30/08, Featherstone, Annette wrote: From: Featherstone, Annette Subject: [Histonet] Quality assurance To: histonet@lists.utsouthwestern.edu Date: Monday, June 30, 2008, 7:59 AM We collect data from our preclinical (accessioning, registration) and document all registration errors, specimen errors, etc. We collect data from our IHC runs such as controls that do not work, wrong antibodies on tissue, wrong cases cut, tissue falling off the slide etc. We do the same for special stains and H & E's. We document all mislabeled blocks and slides. Annette Featherstone Kaleida Health -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Saturday, June 28, 2008 13:02 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 55, Issue 41 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Quality Assurance Measures (Sarah Kolekamp) 2. IHC Marker for differentiation of Human to Mouse hepatocytes (R C) 3. FlexStation 3 needs new home (Erin Curry) 4. MOHS job openings at Loyola University Medical Center (JimR0712@comcast.net) 5. RE: slide cabinets, RA Lamb (Jerry Helisek) 6. HT Positions available (Fidgen, Laura L.) 7. IHC/ISH (Ryan Salazar) 8. slide holders for Lerner Linear Stainer (Barbara Albert) ---------------------------------------------------------------------- Message: 1 Date: Fri, 27 Jun 2008 13:17:33 -0400 From: "Sarah Kolekamp" Subject: [Histonet] Quality Assurance Measures To: Message-ID: <4864E86D0200006100015432@nodcgwcluster_gw4_server.no.trinity-health.org> Content-Type: text/plain; charset=UTF-8 Is anyone currently running a quality assurance program in their histology lab, if so what do you measure/how? We are tring to develop some standards and would like to have data to run analysis on...any ideas on what data to collect?? Thanks, Sarah Kolekamp Sarah Kolekamp HTL (ASCP) Lead Histotechnologist Saint Mary's Health Care Phone Number (616) 752-6155 Fax Number (616) 774-0628 >>> 06/27/08 1:02 PM >>> Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. IHC marker for M-phase cells (Jean-Martin Lapointe) 2. HMB45 antibody (MaryAnn Dixon) 3. Formalin Recyling (John Spair) 4. Re: HMB45 antibody (Jan Shivers) 5. RE: whole mount cryosections (Atoska Gentry) 6. whole mount cryosections (Stephen Peters M.D.) 7. Prisma Stainer/coverslipper (Rebecca Johnson) 8. Vacuum pumps (Jennifer Johnson) 9. RE: IHC marker for M-phase cells (Connolly, Brett M) 10. lab tek metal slide cabinets (Patsy Ruegg) ---------------------------------------------------------------------- Message: 1 Date: Thu, 26 Jun 2008 13:23:17 -0400 From: "Jean-Martin Lapointe" Subject: [Histonet] IHC marker for M-phase cells To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Brett, at my previous job we did IHC staining for phospho-Histone H3 (ser10) using the Cell Signaling rabbit monoclonal antibody CS9701 in FFPE tissue, and it worked beautifully. It was on human origin tissue, don't remember cross-reactivity in other species but Cell Signaling mentions it reacts with many others. Good luck Jean-Martin __________________________________ Jean-Martin Lapointe, DMV, MS, dACVP Vice-President, Pathologie AccelLAB Inc 1635 Lionel-Bertrand, Boisbriand Qu??bec, Canada J7H 1N8 tel:?? 450-435-9482 ext.247 fax: 450-435-4795 jm.lapointe@accellab.com ?? ?? ________________________________________________________________ Message: 6 Date: Thu, 26 Jun 2008 09:27:58 -0400 From: "Connolly, Brett M" Subject: [Histonet] IHC marker for M-phase cells To: , Message-ID: <63EA0607835FBA4689CEA9EA8B48269201306BD2@usctmx1141.merck.com> Content-Type: text/plain; charset="us-ascii" I am looking for an antibody for labeling FFPE sections of mitotic cells that are only in M-phase. Found one article in J Histo Cyto using clone HTA28-phospho-Histone H3(Ser 28) that looked pretty good, but the Authors produced their own antibody. The commercial suppliers I have been able to locate do not indicate IHC as a suitable application for their products. Anyone had experience with this?? Or can you recommend another M-phase specific antibody? Many thanks, Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Research Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com ******************** ------------------------------ Message: 2 Date: Thu, 26 Jun 2008 13:56:05 -0400 From: "MaryAnn Dixon" Subject: [Histonet] HMB45 antibody To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi histonetters, Anyone out there successfully using a monoclonal HMB45 antibody on canine tissues? I think I've read a dozen papers all with different protocols and my eyes are about to pop out of my head! Currently I'm at a 1:50 dilution with a pretreatment in a citrate buffer (pressure cooker). I have my choice of a polymer based detection as well as a biotinylated detection. Any help would be appreciated. Thanks in advance. MaryAnn Dixon BS Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 392-2235 Ext. 4517 ------------------------------ Message: 3 Date: Thu, 26 Jun 2008 10:53:23 -0700 From: "John Spair" Subject: [Histonet] Formalin Recyling To: histonet@lists.utsouthwestern.edu Message-ID: <61A9977919846C479389493BAE2517CA023760EC@MHSEXMBX1.multicare.org> Content-Type: text/plain; charset=us-ascii Is anyone using the formalin recycling/filtration system sold by Creative Waste Solutions?? If you are , please email me at: john.spair@multicare.org if you don't mind, I have some questions. Thank you. "MMS " made the following annotations. ------------------------------------------------------------------------------ NOTICE: This e-mail and the attachments hereto, if any, may contain privileged and/or confidential information. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, you are hereby notified that any examination, distribution or copying of this e-mail and the attachments hereto, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by email or telephone and permanently delete this e-mail and the attachments hereto, if any, and destroy any printout thereof. MultiCare Health System, Tacoma, WA 98415 (253) 403-1000. ============================================================================== ------------------------------ Message: 4 Date: Thu, 26 Jun 2008 15:10:29 -0500 From: "Jan Shivers" Subject: Re: [Histonet] HMB45 antibody To: "MaryAnn Dixon" , Message-ID: Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original I had little or no success with HMB45 on canine tissue. I use Melan-A exclusively; that works fine. Jan Shivers Senior Scientist Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu ----- Original Message ----- From: "MaryAnn Dixon" To: Sent: Thursday, June 26, 2008 12:56 PM Subject: [Histonet] HMB45 antibody Hi histonetters, Anyone out there successfully using a monoclonal HMB45 antibody on canine tissues? I think I've read a dozen papers all with different protocols and my eyes are about to pop out of my head! Currently I'm at a 1:50 dilution with a pretreatment in a citrate buffer (pressure cooker). I have my choice of a polymer based detection as well as a biotinylated detection. Any help would be appreciated. Thanks in advance. MaryAnn Dixon BS Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 392-2235 Ext. 4517 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Thu, 26 Jun 2008 16:52:01 -0500 From: Atoska Gentry Subject: [Histonet] RE: whole mount cryosections To: Histonet Message-ID: <48640F81.7080301@vetmed.auburn.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed hello, will someone with expertise in fresh frozen whole mount and/or coronal primate brain sections please contact me with pointers on requirements for this type sMicrom HM 505 E that will accommodate such samples? We normally section standard size sections but now one of our PI's is considering trying larger samples. Thanks, Atoska ------------------------------ Message: 6 Date: Thu, 26 Jun 2008 15:52:50 -0700 (PDT) From: "Stephen Peters M.D." Subject: [Histonet] whole mount cryosections To: Histonet@lists.utsouthwestern.edu Message-ID: <567183.62199.qm@web45114.mail.sp1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Atoska, ?? Our 50 x 30 mm well bar set?? with 55 x 35 mm chucks may give a bit more room to work. See item # 115 on this page: http://pathologyinnovations.com/Price%20List.htm ?? Stephen ?? ------------------------------ Message: 7 Date: Sat, 26 Jan 2008 22:10:43 -0500 From: "Rebecca Johnson" Subject: [Histonet] Prisma Stainer/coverslipper To: "histonet" Message-ID: <013d01c86092$341481b0$7b48f9d8@CHURCH> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Is anyone using the Prisma coverslipper in Cytology for the Imager? If so how are you doing it? Length of film and level. Thanks Becky ------------------------------ Message: 8 Date: Fri, 27 Jun 2008 09:32:59 -0400 From: Jennifer Johnson Subject: [Histonet] Vacuum pumps To: Message-ID: Content-Type: text/plain; charset="Windows-1252" The James Watson Company sells new & used vacuum pumps. Their phone number is (770) 422-1154. I do not know if they have a web site. They are a direct source instead of having to buy from a medical supply company that will mark up your product. Jennifer Johnson, HTL (ASCP) P.S. I have an affiliation with this company. It is owned by my ex brother-in-law twice removed. _________________________________________________________________ The other season of giving begins 6/24/08. Check out the i??m Talkathon. http://www.imtalkathon.com?source=TXT_EML_WLH_SeasonOfGiving ------------------------------ Message: 9 Date: Fri, 27 Jun 2008 09:42:28 -0400 From: "Connolly, Brett M" Subject: [Histonet] RE: IHC marker for M-phase cells To: , Message-ID: <63EA0607835FBA4689CEA9EA8B48269201306E3A@usctmx1141.merck.com> Content-Type: text/plain; charset="us-ascii" Thanks to all who replied to my question. Seems like everyone uses anti-phosph-histone H3 (Ser10) as I have also used. However, the Ser10 antibody has also been shown to label interphase G2 cells (Goto et al., J Biol Chem 1999, 274: 25543-25549 and others) and I was really looking for an M-phase only marker. So if anyone has used the phospho-Ser28 histone H3 antibody I would be interested to hear your experiences. Brett > _____________________________________________ > From: Connolly, Brett M > Sent: Thursday, June 26, 2008 9:28 AM > To: histonet@lists.utsouthwestern.edu; ihcrg@googlegroups.com > Subject: IHC marker for M-phase cells > > I am looking for an antibody for labeling FFPE sections of mitotic > cells that are only in M-phase. Found one article in J Histo Cyto > using clone HTA28-phospho-Histone H3(Ser 28) that looked pretty good, > but the Authors produced their own antibody. > > The commercial suppliers I have been able to locate do not indicate > IHC as a suitable application for their products. > > Anyone had experience with this?? > > Or can you recommend another M-phase specific antibody? > > Many thanks, > Brett > > Brett M. Connolly, Ph.D. > Research Fellow, Imaging Research > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > PH 215-652-2501 fax. 215-993-6803 > e-mail. brett_connolly@merck.com > Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 10 Date: Fri, 27 Jun 2008 10:17:17 -0600 From: "Patsy Ruegg" Subject: [Histonet] lab tek metal slide cabinets To: "'Histonet'" Message-ID: <005601c8d871$45f86570$6401a8c0@Patsyoffice> Content-Type: text/plain; charset="us-ascii" I have some brand new lab tek metal slide cabinets that are missing the drawer dividers, does anyone know where I can buy these? They turn the drawer into two so you can line up slides or blocks side by side, without these nothing fits in the drawer. Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 55, Issue 40 **************************************** ------------------------------ Message: 2 Date: Fri, 27 Jun 2008 12:10:48 -0700 From: "R C" Subject: [Histonet] IHC Marker for differentiation of Human to Mouse hepatocytes To: histonet@lists.utsouthwestern.edu Message-ID: <2a926e3f0806271210i5c91c94w131a39870892e9f8@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Is there a known Ab that exhibits + expression of hepatacytes in humans but not mice? Thank you. Ruben Carter ------------------------------ Message: 3 Date: Fri, 27 Jun 2008 15:50:49 -0700 From: "Erin Curry" Subject: [Histonet] FlexStation 3 needs new home To: Message-ID: <005c01c8d8a8$3f42d8f0$bdc88ad0$@com> Content-Type: text/plain; charset="us-ascii" Hello all, We are looking for a new home for our Molecular Devices FlexStation 3 multimode microplate reader. It was new in 2006 and has liquid transfer capabilities. If anyone is interested please contact me at the email address below. Erin Curry 1460 O'Brien Drive Menlo Park, CA 94025 (650) 324-2569 erin@aaisolutions.com ------------------------------ Message: 4 Date: Sat, 28 Jun 2008 00:16:26 +0000 From: JimR0712@comcast.net Subject: [Histonet] MOHS job openings at Loyola University Medical Center To: histonet@lists.utsouthwestern.edu (Histonet2), histonet-request@lists.utsouthwestern.edu (Histonet) Message-ID: <062820080016.19416.486582DA0001C92200004BD82216554886CDCEC9CFAD0307B6@comcast.net> Loyola University Medical has 2 immediate opening for MOHS technicians. These openings are the result of opening a second MOHS facility in LaGrange, Illinois. For full details or to apply online, please go to www.luhs.org/depts/hr or contact Joyce Milewski , HR Manager,at 708-216-9409. Jim Robinson, M.S., HTL/HT (ASCP) Manager, Anatomic Pathology ------------------------------ Message: 5 Date: Fri, 27 Jun 2008 20:43:45 -0400 From: "Jerry Helisek" Subject: [Histonet] RE: slide cabinets, RA Lamb To: , Message-ID: <3855F92002259948A66A8CA2D16E3A4F0B5624@server.ralambusa.com> Content-Type: text/plain; charset="us-ascii" Patsy, RA Lamb has drawer dividers that would work if you do not have any luck finding those. Thanks and have a great weekend. ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ ------------------------------ Message: 10 Date: Fri, 27 Jun 2008 10:17:17 -0600 From: "Patsy Ruegg" Subject: [Histonet] lab tek metal slide cabinets To: "'Histonet'" Message-ID: <005601c8d871$45f86570$6401a8c0@Patsyoffice> Content-Type: text/plain; charset="us-ascii" I have some brand new lab tek metal slide cabinets that are missing the drawer dividers, does anyone know where I can buy these? They turn the drawer into two so you can line up slides or blocks side by side, without these nothing fits in the drawer. Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 55, Issue 40 **************************************** ------------------------------ Message: 6 Date: Fri, 27 Jun 2008 17:46:09 -0700 From: "Fidgen, Laura L." Subject: [Histonet] HT Positions available To: Message-ID: <6BB8BC4519AAB844B174FC739A679BBC0148C025@IRMEXCH01.irm.inhs.org> Content-Type: text/plain; charset="iso-8859-1" Hello Histonetters! Here at Sacred Heart Medical Center in beautiful Spokane, WA we have 3 F/T HT positions available. We have 2 day shifts, 05:00-13:30 and 07:00-15:30, and one evening shift, 15:00-23:30. We offer great wages, benefits, and relocation assistance is available. We have state of the art equipment in a newly constructed laboratory coupled with excellent pathologists, pathologist assistants, and laboratory assistants. For more information please visit the hospital website at www.shmc.org. If interested in any of these positions please contact Debbie Ozust, Employment Specialist, at 509-474-3191. Laura Fidgen, HT(ASCP)cm Histology Supervisor Sacred Heart Medical Center 509-474-4106 ------------------------------ Message: 7 Date: Sat, 28 Jun 2008 11:17:24 +0800 From: "Ryan Salazar" Subject: [Histonet] IHC/ISH To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Deareast Histopeeps! We're currently upgrading our clinical research facility into the world of IHC and ISH. Can you provide us ANY BASIC/ FULL EQUIPMENTS/ PROTOCOLS needed for us to be functional in these procedures? We're currently running the routine H&E and some special stains and we have the instruments needed for these. We just want to be ready for our first IHC and ISH stuffs. Thank you for your humble help and your big contribution! Ryan S. Important : This message is intended for the recipient(s) addressed above. It may contain privileged and confidential information. If you are not the intended recipient, please delete it from your system. You must not copy, use, disseminate, disclose, retain or reproduce all or any part of the information or any attachments from this communication in any form. Thank you. ------------------------------ Message: 8 Date: Sat, 28 Jun 2008 08:38:52 -0700 (PDT) From: Barbara Albert Subject: [Histonet] slide holders for Lerner Linear Stainer To: histonet Message-ID: <994234.4916.qm@web63707.mail.re1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi all, Some time ago someone on this list asked if anyone had slide holders for the old Lerner Linerar stainer. Well, we just moved and a box turned up. Let me know if you need them and these brand new slide holders are yours. Barbara Albert Lead Histotechnologist UCSF Medical Center San Francisco 415-353-1610 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 55, Issue 41 **************************************** 2007 Best Places to Work Finalist Visit our careers page at www.kaleidahealth.org/careers CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Mon, 30 Jun 2008 19:04:16 +0530 (IST) From: abi jag Subject: [Histonet] staining manual reg To: histonet@lists.utsouthwestern.edu Message-ID: <469154.7181.qm@web95106.mail.in2.yahoo.com> Content-Type: text/plain; charset=utf-8 Dear Histonetters, I am working as a? histologist in one of the pharmaceutical companies in India. I have accepted an opportunity to set up a histology lab(mainly rodent tissues). I have an query. ? In our archives, i have found some references about Richard Allan website staining manual which is having very useful information for trouble shooting. ? But i didn't find any staining manual and also? Richard Allan is now a part of thermo. ? If any of our fellow histonetters have any idea about this staining manual or its contents. ? thanks for all your help ? ABI ? ? ? ? Explore your hobbies and interests. Go to http://in.promos.yahoo.com/groups/ ------------------------------ Message: 5 Date: Mon, 30 Jun 2008 07:20:23 -0700 From: "Michael Mihalik" Subject: RE: [Histonet] slide printers To: "'Jeanne Estabel'" , Message-ID: <6D3D5A3D481A49ABA9A78EAAD1DED567@MDMM1330> Content-Type: text/plain; charset="US-ASCII" Good morning, You may want to check the archives as there have been a few postings on this subject recently. Depending on your usage, you may want to consider slide LABEL printers rather than slide etchers. In our application, we need the label/slide to be generated just prior to the histotechnologist cutting the tissue. Because this requires a slide printer/labeler to be next to each microtome and because we need these slides/labels to print very quickly, we chose to go with a label printer rather than an etcher. If you would like some details on the printer or would like to hear further details, please let me know. Michael Mihalik PathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeanne Estabel Sent: Monday, June 30, 2008 4:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slide printers Hi, Can I have some feedback on slide printers? Regards Jeanne Jeanne Estabel, PhD MGP Histologist, Team 109 Wellcome Trust/Sanger Institute Hinxton CB10 1SA Tel: 01223 495338 -- The Wellcome Trust Sanger Institute is operated by Genome Research Limited, a charity registered in England with number 1021457 and a compa ny registered in England with number 2742969, whose registered office is 2 15 Euston Road, London, NW1 2BE. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Mon, 30 Jun 2008 10:56:03 -0500 From: "Hector Hernandez" Subject: [Histonet] HT Positions available To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hello Histonetters! Currently PRL (Pathology Reference Lab) is looking for 3 Histology positions to fill. Two will be day shift positions and one will be an evening position 5PM-1:30AM at a satellite lab. We offer excellent benefits and wages will depend on your experience. We are located in warm-hot San Antonio Texas. If interested in any of these positions contact Hector Hernandez at (210)892-3747. Hector Hernandez, HT (ASCP) Histology Supervisor 210-892-3747 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 55, Issue 42 **************************************** This communication is intended for the use of the recipient to which it is addressed, and may contain confidential, personal and or privileged information. Please contact us immediately if you are not the intended recipient. Do not copy, distribute or take action relying on it. Any communication received in error, or subsequent reply, should be deleted or destroyed. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Mon Jun 30 12:39:25 2008 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Mon Jun 30 12:39:31 2008 Subject: [Histonet] QA documentation Message-ID: <0E394B648E5284478A6CCB78E5AFDA27056357ED@hpes1.HealthPartners.int> We document all deficiencies from the clinic, such as leaky containers, mislabeling, no patient clinical information, demographics and pull quarterly reports which gives us data to work with the clinics and hospital departments with. We also collect from the pathologist's input, technical problems, such as floaters, mislabeling, inadequate fixation, processing, or decal, knife lines or tissue folds, poor stain quality, improper embedding and pull out a monthly report on this data. The other quality we look at monthly is data from accessioning and billing, on both the histotech and pathologist's quality. Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From freckles9660 <@t> yahoo.com Mon Jun 30 12:42:40 2008 From: freckles9660 <@t> yahoo.com (Karla Arrington) Date: Mon Jun 30 12:42:44 2008 Subject: [Histonet] histo speed Message-ID: <440470.56186.qm@web32504.mail.mud.yahoo.com> How many cassettes should a tech be able to embed in an hour as well as how many blocks should a tech cut in an hour? Doing a control study. Thanks! Karla Arrington, HT(ASCP) freckles9660@yahoo.com From epoc101 <@t> gmail.com Mon Jun 30 13:36:03 2008 From: epoc101 <@t> gmail.com (Joe) Date: Mon Jun 30 13:36:11 2008 Subject: [Histonet] Leica Autostainer XL for Sale Message-ID: <231dd8040806301136m42c962b8p8df5f0224105a80d@mail.gmail.com> I have a used Leica Autostainer XL, used, in fair to good condition, for sale. This is a workhorse stainer that was running until we recently upgraded to a new stainer. It's about 9 years old. I would like to see it get a good home. Asking $5,000 or best offer. You pay shipping or pick up. Please reply directly to EPOC101@gmail.com. Thank you. From rjbuesa <@t> yahoo.com Mon Jun 30 13:58:31 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jun 30 13:58:37 2008 Subject: [Histonet] Speed In-Reply-To: <635818.32288.qm@web32505.mail.mud.yahoo.com> Message-ID: <498979.64681.qm@web65711.mail.ac4.yahoo.com> US averages are: 24 blocks / hour sectioned, 60 blocks / hour embedded. Ren? J. --- On Mon, 6/30/08, Karla Arrington wrote: From: Karla Arrington Subject: [Histonet] Speed To: histonet@lists.utsouthwestern.edu Date: Monday, June 30, 2008, 1:19 PM Histo's: ?Generally speaking, what is the routine number of?tissues (cassettes)?a tech should embed in an hour? Also how many blocks should a tech be able to cut in an hour? Thanks, Karla Arrington _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Mon Jun 30 18:23:20 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Mon Jun 30 18:23:27 2008 Subject: [Histonet] Digesters PLEASE TRIM! Message-ID: <582736990806301623l4747a29ew95ecbd96d9a71d2@mail.gmail.com> Hey, I've been guilty of this on occasion as well, but for the love of sanity PLEASE TRIM YOUR POSTS! The digest version is completly unreadable when folks subscribed to digest reply to a post without trimming the original post. (Just highlight the text and hit [delete] ) Non digest folks, you aren't off the hook either. Replying to an untrimmed digest is just as bad as not trimming it in the first place. (Please see the above trimming directions.) There really is no reason that the digest (usually carrying 20-25 messages) should be so filled up with clutter that only 3 messages come thru. Please be respectful of the amount of time it takes to wade thru all this. This forum is too great a resource to be wasted on pointless & careless wasted space. A bit of Nettiquette please, Amos PS I am truly sorry if this comes across sharply, but it really needs to stop. From amylee779 <@t> yahoo.com Mon Jun 30 18:30:46 2008 From: amylee779 <@t> yahoo.com (Amy Lee) Date: Mon Jun 30 18:30:49 2008 Subject: [Histonet] Quantification for Sirius red staining Message-ID: <544175.7093.qm@web38005.mail.mud.yahoo.com> Hello histonetters, ? I've asked a few questions here and got many big help. I appreciated all help you guys provided. ? I have another question. I've done sirius red stain on paraffin sections and need quantify it. I have image pro plus software. So I am thinking use it to measure sirius red density. I am trying to find reference that other people done that before (to support my idea).? Have you or anybody you know done that?before? I googled but?not many helpful info availble. ? Thanks for your help! ? Amy