From anh2006 <@t> med.cornell.edu Tue Jul 1 00:15:06 2008 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Tue Jul 1 00:15:12 2008 Subject: [Histonet] VCAM, VLA-4, ICAM, E-selectin for mouse Message-ID: I want to stain for VCAM (CD106), VLA-4 (CD49D), ICAM (CD54), E-selectin (CD62E) for mouse tissue. Any suggestions of what Abs to use. I can do PFA fixed frozens or FFPE but would prefer FFPE. Thank you! ANDREA -- From mickie25 <@t> netzero.net Tue Jul 1 06:45:37 2008 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Tue Jul 1 06:45:22 2008 Subject: [Histonet] A Question about CLIA Regs and Grossing in private Dermpath Labs In-Reply-To: References: Message-ID: Dear Histonetters, I have a question to pose out there to all the private histology labs, but particularly the dermpath labs. CLIA deems 'grossing' to be high complexity testing. This has been discussed in the histonet before at length as recently as 2007. Out of that discussion there was mention of the requirement of 60 credits of education plus 3 months training. The following relates to an interpretive guideline published in 2003. The following is extracted from a histonet archive in March of 2006. ?www.cms.hhs.gov/clia is the link to the CLIA '88 information. See the new federal register CLIA interpretive guidelines appendix C subpart M that are effective April 24, 2003. The guidelines are available at www.cms.hhs.gov/CLIA/downloads/apcsubm.pdf under qualifications of high complexity testing personnel section 493.1489 (b)(7) it is clear that all tissue gross examination whether it is color, measurement, or advanced dissection is considered high complexity testing and individuals performing this type of testing must qualify under this section. That is why the new CAP question ANP 11610 was instated and is effective April 28, 2005. CAP must abide by CLIA regulations and CLIA is part of the Center for Medicare and Medicaid Services which is a section of the US government's department of Health and Human Services. So if you would like to maintain your CLIA license and CAP certification and be paid by Medicare you must abide by the requirements for high complexity testing personnel for each person performing any kind of gross examination. The actual wording is: "Interpretive Guidelines ?493.1489(b)(7)In the case of gross examinations, the technical supervisor may delegate to individuals qualified under ?493.1489 the responsibility for the physical examination/description, including color, weight, measurement and other characteristics of the tissue; or other mechanical procedures for which a specific written protocol has been developed. The technical supervisor is ultimately responsible for the diagnosis related to the gross examination and must sign the examination report. The technical supervisor is not required to provide direct onsite supervision but is responsible for the accuracy of all test results reported. All physical examinations/descriptions of tissue including color, weight, measurement and other characteristics of the tissue; or other mechanical procedures performed in the absence of the technical supervisor by individuals qualified under ?493.1489 should be reviewed within 24 hours by the technical supervisor. All microscopic tissue examinations must be performed by individuals qualified under ?493.1449(b), (l) or (m), as appropriate."? As I understand this, the technical supervisor (in this case the dermatologist (or other physician) can or does take responsibility for supervising grossing of small biopsies such as derm biopsies and can delegate this to other personnel if supervised directly or indirectly within 24 hours. My question is how is this being written up in procedure form so that it is not rejected by the CLIA inspector. Does this interpretation require the supervised technician doing the grossing to have 60 credits of education if there is a detailed written guidelines for submitting the specimens in toto as is done with dermatological specimens? Thank you in advance for your help. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From rjbuesa <@t> yahoo.com Tue Jul 1 07:14:21 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jul 1 07:14:25 2008 Subject: [Histonet] Quantification for Sirius red staining In-Reply-To: <544175.7093.qm@web38005.mail.mud.yahoo.com> Message-ID: <373099.56875.qm@web65714.mail.ac4.yahoo.com> Amy: Regardless of somebody or nobody doing that, it is a good idea and should be done. The only thing is that you have to have a reference point that should be a KNOWN concentration reacted with the stain to refer to your determinations. Ideally you should have a low and high concentrations and determine a correlation, in the same way as with a colorimetric determination of the concentration of some substance with a colored reaction. Ren? J. --- On Mon, 6/30/08, Amy Lee wrote: From: Amy Lee Subject: [Histonet] Quantification for Sirius red staining To: "histonet" Date: Monday, June 30, 2008, 7:30 PM Hello histonetters, ? I've asked a few questions here and got many big help. I appreciated all help you guys provided. ? I have another question. I've done sirius red stain on paraffin sections and need quantify it. I have image pro plus software. So I am thinking use it to measure sirius red density. I am trying to find reference that other people done that before (to support my idea).? Have you or anybody you know done that?before? I googled but?not many helpful info availble. ? Thanks for your help! ? Amy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Tue Jul 1 07:37:36 2008 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Jul 1 07:37:40 2008 Subject: [Histonet] Histo Techs needed near Wilmington NC and Sacramento, CA and a histology supervisor needed in Buffalo, NY Message-ID: Hi Histonetters, I hope everybody is getting ready for a fun packed 4th of July weekend. I just wanted to post a quick notice about a couple of positions I am working on. These are permanent positions. Near Wilmington, NC - 3rd shift histo tech Sacramento - Day Shift Histo tech with strong cutting skills Buffalo, NY - Histology Supervisor for prestigious organization. My clients offer excellent salaries, benefits and relocation assistance. If you would like more detailed information for yourself or know of someone else who might be interested please contact me or forward my information along. Thanks!! Have a great day and a safe 4th of July - Pam I also have some great opportunities in FL, TX, WA, MD, Pittsburgh. PA and Los Angeles, CA Thank You! Pam M. Barker President Relia 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll Free: (866)607-3542 e-mail: relia1@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia From sonyprasad <@t> hotmail.com Tue Jul 1 08:39:38 2008 From: sonyprasad <@t> hotmail.com (sony prasad) Date: Tue Jul 1 08:39:44 2008 Subject: [Histonet] peroxidase anti-peroxidase staining Message-ID: Dear All, Has anyone used a PAP staining method for FFPE mouse tissues for VCAM-1. Please could you let me have a protocol with what antibodies you would recommend. Thanks, Sony _________________________________________________________________ No Harvard, No Oxford. We are here. Find out !! http://ss1.richmedia.in/recurl.asp?pid=500 From algranth <@t> u.arizona.edu Tue Jul 1 09:45:39 2008 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Tue Jul 1 09:45:53 2008 Subject: [Histonet] Quantification for Sirius red staining In-Reply-To: <373099.56875.qm@web65714.mail.ac4.yahoo.com> References: <544175.7093.qm@web38005.mail.mud.yahoo.com> <373099.56875.qm@web65714.mail.ac4.yahoo.com> Message-ID: <6.2.3.4.1.20080701074002.01f8d6b8@algranth.inbox.email.arizona.edu> I haven't done this but I have done the Picrosirius stain for people who have. What I do is this - Don't use a counterstain. Our imaging expert says to use a grayscale camera. I just googled quantification of picrosirius red and got several hits - you might want to try this and see if any will give you a protocol. Andi At 05:14 AM 7/1/2008, Rene J Buesa wrote: >Amy: >Regardless of somebody or nobody doing that, it >is a good idea and should be done. >The only thing is that you have to have a >reference point that should be a KNOWN >concentration reacted with the stain to refer to >your determinations. Ideally you should have a >low and high concentrations and determine a >correlation, in the same way as with a >colorimetric determination of the concentration >of some substance with a colored reaction. >Ren? J. > >--- On Mon, 6/30/08, Amy Lee wrote: > >From: Amy Lee >Subject: [Histonet] Quantification for Sirius red staining >To: "histonet" >Date: Monday, June 30, 2008, 7:30 PM > >Hello histonetters, > >I've asked a few questions here and got many big help. I appreciated all >help you guys provided. > >I have another question. I've done sirius red stain on paraffin sections >and need quantify it. I have image pro plus software. So I am thinking use it >to measure sirius red density. I am trying to find reference that other people >done that before (to support my idea). Have you or anybody you know done >that before? I googled but not many helpful info availble. > >Thanks for your help! > >Amy > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From Histowa13 <@t> aol.com Tue Jul 1 09:58:58 2008 From: Histowa13 <@t> aol.com (Histowa13@aol.com) Date: Tue Jul 1 09:59:12 2008 Subject: [Histonet] ihc stainers Message-ID: Hi, I was wondering if anyone was using any of the new IHC stainers out there. We are in the market for a different stainer but have not had much luck in finding folks who are using the newer machines. Debbie Nannenga, HTL, QIHC InCyte Pathology 13103 E Mansfield Spokane Valley, WA 99216


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(http://autos.aol.com/used?ncid=aolaut00050000000007) From rjbuesa <@t> yahoo.com Tue Jul 1 10:20:59 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jul 1 10:21:05 2008 Subject: [Histonet] ihc stainers In-Reply-To: Message-ID: <838970.12600.qm@web65702.mail.ac4.yahoo.com> Debbie: What is exactly that you mean with "newer machines"? All IHC stainers are just either with "open" or "closed technologies", all are "walk away" and some can receive the section and complete the whole process from dewaxing to counterstain, and others need the section after HIER and will finish with the detection system. And that has?been done with machines that began appearing in the market since more than 5 years ago, and that I would not qualify as "newer". Perhaps you are referring to one of those technologies and not to the machine itself. Ren? J. --- On Tue, 7/1/08, Histowa13@aol.com wrote: From: Histowa13@aol.com Subject: [Histonet] ihc stainers To: histonet@lists.utsouthwestern.edu Date: Tuesday, July 1, 2008, 10:58 AM Hi, I was wondering if anyone was using any of the new IHC stainers out there. We are in the market for a different stainer but have not had much luck in finding folks who are using the newer machines. Debbie Nannenga, HTL, QIHC InCyte Pathology 13103 E Mansfield Spokane Valley, WA 99216


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(http://autos.aol.com/used?ncid=aolaut00050000000007) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcline <@t> wchsys.org Tue Jul 1 11:53:48 2008 From: jcline <@t> wchsys.org (Joyce Cline) Date: Tue Jul 1 11:53:57 2008 Subject: [Histonet] slide printers In-Reply-To: <0526C4B4E593154B86E6A8651913C28D0245360E@exchsrv2.internal.sanger.ac.uk> Message-ID: I have a Leica Slide printer. We have a few problems every now and then but it is well worth not having to hand write the slides. The printer has three slide holders but you can order more holders, we have 6 holders to cover the different colored slides that we use. We opted for the attached slide rack holder, it holds 10 trays of printed slides. ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From yangli <@t> EVMS.EDU Tue Jul 1 13:03:45 2008 From: yangli <@t> EVMS.EDU (Yang, Li Fang) Date: Tue Jul 1 13:04:12 2008 Subject: [Histonet] lectin histochemistry questions Message-ID: Dear all, I am a newer to lectin histochemistry. I need to work up a portocol using a panel of lectins for human prostate cancer tissue sections. I was advised to use fresh snap-frozen tissue, and fluorence conjugated lectin as a start point. Now I have 7 FITC conjugated lectins and frozen sections. But I still have some questions to ask for your help. 1. What is the suitable fixtive for the fresh tissue? 75%acetone? 25%ethonal? or ?%methonal? 2. Do I need to dehydrated sections? before or after fixation? 3. Do I need to block endogenous peroxidase if I don't use peroxidase-based detection? 4. Is it necessary to use some blocking reagents such as BSA in the staining buffer (lectin buffer as suggested in this website)? I tried 2% FBS/PBS and 1%BSA/PBS in the lectin staining by flow cytometry. They both saturated the binding of lectins, almost to the same extent. FBS contains a lot glycan residures, but how to explain the effect of BSA? it is even not a glycoprotein. 5. It is said that FITC may conflict with some autofluorescence in FFPE sections. How about in fresh sections? how to figure it out? 6. How to evaluate the staining intensity? Is there a specific software required? 7. For lectin specificity, I plan to use inhibitory sugar. anybody know the solvents for lactose and N-acetylneraminic acid? I try to make 1M stock solution. They seemed not dissolved in water. Sorry for so many questions. Any tips and protocol sharing would be greatly appreciated. :: From schaundrawalton <@t> yahoo.com Tue Jul 1 13:31:04 2008 From: schaundrawalton <@t> yahoo.com (Schaundra Walton) Date: Tue Jul 1 13:31:11 2008 Subject: [Histonet] Re: Speed Message-ID: <129182.49967.qm@web58915.mail.re1.yahoo.com> I have my techs average 50-60 blocks/hour embedding and 20/hour cutting. ? Schaundra Walton BS, HTL (ASCP) Histology Supervisor Swedish American Hospital Rockford, IL ? ? ? Date: Mon, 30 Jun 2008 10:42:40 -0700 (PDT) From: Karla Arrington Subject: [Histonet] histo speed To: histonet@lists.utsouthwestern.edu Message-ID: <440470.56186.qm@web32504.mail.mud.yahoo.com> Content-Type: text/plain; charset=us-ascii How many cassettes should a tech be able to embed in an hour as well as how many blocks should a tech cut in an hour? Doing a control study. Thanks! Karla Arrington, HT(ASCP) freckles9660@yahoo.com From Stanley.Lupo <@t> gsk.com Tue Jul 1 13:35:16 2008 From: Stanley.Lupo <@t> gsk.com (Stanley.Lupo@gsk.com) Date: Tue Jul 1 13:35:36 2008 Subject: [Histonet] Re: Histonet Digest, Vol 56, Issue 1 In-Reply-To: <200807011652.m61GqY0j003868@rtpsfimr002.glaxo.com> Message-ID: Histonetters, We have five one gallon bottles of Lerner Laboratories' Harris Hematoxylin (expiry: 11-12, 2008) that we are willing to donate rather than have them expire in our lab. (We are now using the Ventana Symphony for H&E staining.) Please contact me if you can use these stains. Stanley.Lupo@GSK.Com Safety Assessment Mail Code: UE0461 Phone: 610 270-7340 Fax: 610 270-7202 From rjbuesa <@t> yahoo.com Tue Jul 1 14:03:55 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jul 1 14:04:03 2008 Subject: [Histonet] lectin histochemistry questions In-Reply-To: Message-ID: <151248.71462.qm@web65701.mail.ac4.yahoo.com> Li Fang: As per your questions: 1- use acetone 2- if they are FS you do not need to dehydrate, you need to place them in a buffer (PBS is advisable) 3- you do not need to block, remember that your lectin is the one that is going to be "added" to the tissue for a later detection I cannot advise on the remaining questions Ren? J. --- On Tue, 7/1/08, Yang, Li Fang wrote: From: Yang, Li Fang Subject: [Histonet] lectin histochemistry questions To: histonet@lists.utsouthwestern.edu Date: Tuesday, July 1, 2008, 2:03 PM Dear all, I am a newer to lectin histochemistry. I need to work up a portocol using a panel of lectins for human prostate cancer tissue sections. I was advised to use fresh snap-frozen tissue, and fluorence conjugated lectin as a start point. Now I have 7 FITC conjugated lectins and frozen sections. But I still have some questions to ask for your help. 1. What is the suitable fixtive for the fresh tissue? 75%acetone? 25%ethonal? or ?%methonal? 2. Do I need to dehydrated sections? before or after fixation? 3. Do I need to block endogenous peroxidase if I don't use peroxidase-based detection? 4. Is it necessary to use some blocking reagents such as BSA in the staining buffer (lectin buffer as suggested in this website)? I tried 2% FBS/PBS and 1%BSA/PBS in the lectin staining by flow cytometry. They both saturated the binding of lectins, almost to the same extent. FBS contains a lot glycan residures, but how to explain the effect of BSA? it is even not a glycoprotein. 5. It is said that FITC may conflict with some autofluorescence in FFPE sections. How about in fresh sections? how to figure it out? 6. How to evaluate the staining intensity? Is there a specific software required? 7. For lectin specificity, I plan to use inhibitory sugar. anybody know the solvents for lactose and N-acetylneraminic acid? I try to make 1M stock solution. They seemed not dissolved in water. Sorry for so many questions. Any tips and protocol sharing would be greatly appreciated. :: _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leahcox27 <@t> yahoo.com Tue Jul 1 14:25:33 2008 From: leahcox27 <@t> yahoo.com (Leah Cox) Date: Tue Jul 1 14:25:39 2008 Subject: [Histonet] refilling biopsy bottles Message-ID: <303145.14539.qm@web55008.mail.re4.yahoo.com> Does anyone know if it's legal to wash and refill formalin biopsy bottles to use from one patient to another? I can't find the info on OSHA or any other web site. From japoteete <@t> saintfrancis.com Tue Jul 1 14:43:13 2008 From: japoteete <@t> saintfrancis.com (Poteete, Jacquie A.) Date: Tue Jul 1 14:43:23 2008 Subject: [Histonet] ihc stainers In-Reply-To: Message-ID: If "new" includes the new DAKO AutostainerLink 48, then yes, we are. It's about the newest you can get and we just got our third one. I'm very impressed, and the software is drastically improved over the old Autostainers. Jacquie Poteete MT(ASCP)QIHC IHC Lead Technologist Saint Francis Hospital Tulsa, OK japoteete@saintfrancis.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Histowa13@aol.com Sent: Tuesday, July 01, 2008 9:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ihc stainers Hi, I was wondering if anyone was using any of the new IHC stainers out there. We are in the market for a different stainer but have not had much luck in finding folks who are using the newer machines. Debbie Nannenga, HTL, QIHC InCyte Pathology 13103 E Mansfield Spokane Valley, WA 99216


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(http://autos.aol.com/used?ncid=aolaut00050000000007) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jamie.erickson <@t> abbott.com Tue Jul 1 14:53:42 2008 From: jamie.erickson <@t> abbott.com (Jamie E Erickson) Date: Tue Jul 1 14:53:53 2008 Subject: [Histonet] Quantification for Sirius red staining In-Reply-To: <61gkfa$a535il@vette.abbott.com> Message-ID: Hi Amy, I have done this using polarized light with Image pro plus. Also here is a brief procedure of what I did, I was trying to quantitate collagen in mouse disease lungs. You should remember if you are trying to quantitate this let your scope warm up for 30 minutes prior to capturing your images and keep all light settings (levels) / image size the same. Your lamp temperature as it warms can give you different reading (IOD) if not warmed up. I used a polarizer that screwed into to my scope so I could rotate it to the right degree (I think 45) and it sits in place so as not to move. I also did not use any NDF filters and I used the mouse Kidney as positive control. Here is a reference that I followed. 1. Gaoyun Yan, 2005 Therapeutic Dosing of anti-IL-13 Monoclonal Antibody inhibits Asthma Progression in Mice. Journal of Pharmacology and Experimental Therapeutics. 313: 8-15. 2. Juqueira LC, Picrosirius staining plus polarization microscopy, a specific method for collagen detection in tissue sections. J. Histochemistry 11(4) 447-55, 1979. 3. Histonet search: http://www.histosearch.com/histonet/Jul01A/Siriusredcollagenprocedur.thml picrosirius red (PSR) (EMS, Cat #26357) for Collagen deposition. Picosirious Red Imaging Slides where stained, imaged and saved as stated above. Images for PSR collagen deposition where photographed under polarized light microscopy, which yields a yellow, red and green birefringence of collagens type I, II, III (Appendix 3). Background non-specific birefringence is removed as stated above. Images are converted from RGB color images to HSI images and the Intensity channel is used for analysis. The images are calibrated and Threshholded on the red and yellow collagen fibers which represent mostly the type I fibers. These areas are measured and counted using the integrated optical density (IOD) feature, which represents the area multiplied by the average intensity. This measurement is then exported to excel and the two images for each sample are averaged to arrive at the average intensity for the total sample. Hope it helps. Jamie From rjbuesa <@t> yahoo.com Tue Jul 1 15:00:28 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jul 1 15:00:31 2008 Subject: [Histonet] refilling biopsy bottles In-Reply-To: <303145.14539.qm@web55008.mail.re4.yahoo.com> Message-ID: <228047.5371.qm@web65707.mail.ac4.yahoo.com> Even if it is legal, it is highly unwise to handle formalin more than just the necessary. Besides that you have to have a very well documented protocol that ABSOLUTELY assures that there cannot be any carryover from one patient to the next. I highly advise you to discard that idea! Ren? J. --- On Tue, 7/1/08, Leah Cox wrote: From: Leah Cox Subject: [Histonet] refilling biopsy bottles To: histonet@lists.utsouthwestern.edu Date: Tuesday, July 1, 2008, 3:25 PM Does anyone know if it's legal to wash and refill formalin biopsy bottles to use from one patient to another? I can't find the info on OSHA or any other web site. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From renafail <@t> bellsouth.net Tue Jul 1 15:03:30 2008 From: renafail <@t> bellsouth.net (Rena Fail) Date: Tue Jul 1 15:03:35 2008 Subject: [Histonet] refilling biopsy bottles In-Reply-To: <303145.14539.qm@web55008.mail.re4.yahoo.com> Message-ID: <000001c8dbb5$891b2440$0301a8c0@RENAD4YK9B8ABE> If is not illegal, it ought to be. In my opinion reusing a biopsy container would be courting disaster. Rena Fail -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Leah Cox Sent: Tuesday, July 01, 2008 3:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] refilling biopsy bottles Does anyone know if it's legal to wash and refill formalin biopsy bottles to use from one patient to another? I can't find the info on OSHA or any other web site. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Tue Jul 1 15:17:23 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Tue Jul 1 15:17:22 2008 Subject: [Histonet] Tricore Laboratory NM Message-ID: <486A90D3.7000900@pathology.washington.edu> Anyone on the Histonet employed at Tricore? Please contact me directly as I have a questions regarding the General Data cassette labeler. -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From marktarango <@t> gmail.com Tue Jul 1 17:45:42 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Tue Jul 1 17:45:48 2008 Subject: [Histonet] refilling biopsy bottles In-Reply-To: <303145.14539.qm@web55008.mail.re4.yahoo.com> References: <303145.14539.qm@web55008.mail.re4.yahoo.com> Message-ID: <5b6eb13e0807011545p7be7c0bq31b84c4d362bdec9@mail.gmail.com> I'm curious as to your procedure for washing the bottles out... Is it just soap and water? I've worked in more than one lab that washed bottles out (it was always a derm lab now that i think about it). Mark Tarango On Tue, Jul 1, 2008 at 12:25 PM, Leah Cox wrote: > Does anyone know if it's legal to wash and refill formalin biopsy bottles > to use from one patient to another? I can't find the info on OSHA or any > other web site. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ari.liebkowsky <@t> charite.de Wed Jul 2 05:05:08 2008 From: ari.liebkowsky <@t> charite.de (Ari Liebkowsky) Date: Wed Jul 2 05:05:18 2008 Subject: [Histonet] Paraffin blocks (reprise) Message-ID: <486B52D4.8030909@charite.de> We have a collection of several thousand paraffin blocks (unsealed), and an arachnid has been found in some. The blocks date back as early as the 70's and are currently stored in small cardboard boxes, 5-10 at a time, which are themselves stored in plastic storage containers with lids. Some of the cardboard boxes indicate moisture damage, though when and how this occurred is unclear. The plastic containers are in a cellar without direct sunlight or temperature control. The room seems a little damp, but testing indicates the humidity was in the normal range (for office workers, not necessarily paraffin blocks). It can also warm up a bit in winter as heating pipes run through the room. I'm looking for the best way to get rid of the bugs, while creating the best conditions for the collection longterm. Two options have been presented: 1. Fumigating the collection. This seems to be the simplest, and we could simply leave the blocks in their current storage containers. 2. Obtaining fridges, putting the blocks into ziplock bags, storing the blocks at -5 Cel. for several weeks to kill the bugs and then storing the bags at about 4 Cel. from then on. The second option is a bit of an issue, space and money-wise and I'm wondering whether it's worth it. There don't seem to be many (any) studies on such longterm storage of paraffin blocks. The blocks do/will get used, and some (limited) feedback I've received is that the signal strength is def. stronger in the more recent blocks than the ones from the 1980s. Would -5 Cel really kill the bugs (and their eggs)? Any exp. with dampness and temperature control for blocks? Any feedback appreciated. Cheers, James From rjbuesa <@t> yahoo.com Wed Jul 2 07:28:45 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jul 2 07:28:52 2008 Subject: [Histonet] Paraffin blocks (reprise) In-Reply-To: <486B52D4.8030909@charite.de> Message-ID: <312491.16180.qm@web65711.mail.ac4.yahoo.com> James: First of all let me express you my complete amazement when confronted with a block collection more than 30 years old; it seems to me than, unless it is really frequent that those blocks are used for research purposes, it is a real useless effort and waste of space. If they are regularly used then it is OK. ? I think that the fumigation will be the best option. ? As to the effect of?long term storage of blocks I would like to work in your institution to do that research. I imagine that if you have kept the blocks you have also the case reports, then I would do as follows: 1- select a pathological entity that would require some IHC testing, lets say melanoma cases. 2- select?1 case for each of the years you have in storage 3- find the blocks and run an H&E and a HMB45 for each and analyze how the Ag has "resisted" the storage. That would allow me to answer the storage question and, perhaps, to eliminate all the years that show such a weakening of the Ag that is not worth its safeguard any longer. Just my opinion! Ren? J. On Wed, 7/2/08, Ari Liebkowsky wrote: ? From Carol.Fields <@t> Northside.com Wed Jul 2 08:45:14 2008 From: Carol.Fields <@t> Northside.com (Carol Fields) Date: Wed Jul 2 08:45:42 2008 Subject: [Histonet] Water bath temps Message-ID: <038809F21D1F9040A1FEAD72A7E2C6B50972463D@NSMXMS04.northside.local> Hi Netters, We received a recommendation.. for not having water bath temp ranges (QC chart) on our last CAP inspection. I thought this was taken off the list? What range do you all have on your QC sheets for your water bath temps? Thanks in advance, Carole Fields Northside Hospital Atlanta, GA CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. From brett_connolly <@t> merck.com Wed Jul 2 08:46:24 2008 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Wed Jul 2 08:46:38 2008 Subject: [Histonet] PhosphoGuard fixative additive Message-ID: <63EA0607835FBA4689CEA9EA8B48269201307359@usctmx1141.merck.com> In the June 2008 issue of CAP Today, I ran across a fixative additive called PhosphoGuard from Targeted Molecular Technologies. They claim it prevents degradation of phosphorylated and activated proteins allowing for better IHC detection (stronger/more abundant signal) by better inactivation of phosphatases compared to plain 10% NBF. Apparently you just add some to your formalin. You can check it out further on their website. http://www.tmdlab.com/technologies_PHOSPHO.htm Just wondering if any of you have tried it? Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Research Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From koellingr <@t> comcast.net Wed Jul 2 09:26:25 2008 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Wed Jul 2 09:26:54 2008 Subject: [Histonet] lectin histochemistry questions Message-ID: <070220081426.16051.486B901100091E6200003EB322007637049D09020704040A0105@comcast.net> Li Fang, In addition to some of the other comments, do not forget the role of the buffer in lectin histochemistry. Do not know which lectins you are using on your prostate study but some lectins are problematic in their need for divalent cations (calcium or magnesium) in buffers for binding to the sugar residue. Some lectins are no problem and can use PBS but some are sensitive and some (I think those targeting mannose?) absolutely require divalent cations. Since you can't be putting such stuff in PBS without precipitating out the phosphate salt, a lot of people use Hepes buffers. See the Vector website which in my opinion, although I have absolutely no connection with Vector, is the premier place to go for information on lectin histochemistry staining. Ray Koelling PhenoPath Labs Seattle, WA -------------- Original message ---------------------- From: Rene J Buesa > Li Fang: > As per your questions: > 1- use acetone > 2- if they are FS you do not need to dehydrate, you need to place them in a > buffer (PBS is advisable) > 3- you do not need to block, remember that your lectin is the one that is going > to be "added" to the tissue for a later detection I cannot advise on the > remaining questions > René J. > > --- On Tue, 7/1/08, Yang, Li Fang wrote: > > From: Yang, Li Fang > Subject: [Histonet] lectin histochemistry questions > To: histonet@lists.utsouthwestern.edu > Date: Tuesday, July 1, 2008, 2:03 PM > > Dear all, > > I am a newer to lectin histochemistry. I need to work up a portocol using a > panel of lectins for human prostate cancer tissue sections. I was advised to > use fresh snap-frozen tissue, and fluorence conjugated lectin as a start point. > Now I have 7 FITC conjugated lectins and frozen sections. But I still have some > questions to ask for your help. > 1. What is the suitable fixtive for the fresh tissue? 75%acetone? 25%ethonal? > or ?%methonal? > 2. Do I need to dehydrated sections? before or after fixation? > 3. Do I need to block endogenous peroxidase if I don't use peroxidase-based > detection? > 4. Is it necessary to use some blocking reagents such as BSA in the staining > buffer (lectin buffer as suggested in this website)? I tried 2% FBS/PBS and > 1%BSA/PBS in the lectin staining by flow cytometry. They both saturated the > binding of lectins, almost to the same extent. FBS contains a lot glycan > residures, but how to explain the effect of BSA? it is even not a glycoprotein. > 5. It is said that FITC may conflict with some autofluorescence in FFPE > sections. How about in fresh sections? how to figure it out? > 6. How to evaluate the staining intensity? Is there a specific software > required? > 7. For lectin specificity, I plan to use inhibitory sugar. anybody know the > solvents for lactose and N-acetylneraminic acid? I try to make 1M stock > solution. They seemed not dissolved in water. > > Sorry for so many questions. Any tips and protocol sharing would be greatly > appreciated. > > > > :: > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr <@t> comcast.net Wed Jul 2 09:38:52 2008 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Wed Jul 2 09:39:26 2008 Subject: [Histonet] PhosphoGuard fixative additive Message-ID: <070220081438.2966.486B92FC0003732C00000B9622007637049D09020704040A0105@comcast.net> Brett, Although I have not used this and do not know anything about this product, in a former life I did something like it. When starting to work with phospho targets years ago in IHC was worried about the transient nature of phosphorylation/dephosphorylation. Do Westerns to ID target and you are under the constraint of time and adding phosphate inhibitors to your lysate so your target doesn't degrade. So how did this relate to a tissue block where fixation is not immediate throughout. I started adding reversible inhibitors or non-reversible inhibitors to fixatives and did in a sense find what we sought. In some cases, couldn't improve results. But for sure, some specific phosphorylated targets we absolutely saw better and stronger signal and in some cases went from no/poor signal to very good signal. So I think the theory for why the product should work is sound and we did this a lot in a proprietary sense, but again do not know about this specific product. Ray Koelling PhenoPath Labs Seattle, WA -------------- Original message ---------------------- From: "Connolly, Brett M" > In the June 2008 issue of CAP Today, I ran across a fixative additive > called PhosphoGuard from Targeted Molecular Technologies. > > They claim it prevents degradation of phosphorylated and activated > proteins allowing for better IHC detection (stronger/more abundant > signal) by better inactivation of phosphatases compared to plain 10% > NBF. Apparently you just add some to your formalin. > > You can check it out further on their website. > http://www.tmdlab.com/technologies_PHOSPHO.htm > > Just wondering if any of you have tried it? > > Brett > > Brett M. Connolly, Ph.D. > Research Fellow, Imaging Research > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > PH 215-652-2501 fax. 215-993-6803 > e-mail. brett_connolly@merck.com > > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, > New Jersey, USA 08889), and/or its affiliates (which may be known > outside the United States as Merck Frosst, Merck Sharp & Dohme or > MSD and in Japan, as Banyu - direct contact information for affiliates is > available at http://www.merck.com/contact/contacts.html) that may be > confidential, proprietary copyrighted and/or legally privileged. It is > intended solely for the use of the individual or entity named on this > message. If you are not the intended recipient, and have received this > message in error, please notify us immediately by reply e-mail and > then delete it from your system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Jul 2 10:54:35 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jul 2 10:54:38 2008 Subject: [Histonet] Water bath temps In-Reply-To: <038809F21D1F9040A1FEAD72A7E2C6B50972463D@NSMXMS04.northside.local> Message-ID: <410640.51877.qm@web65712.mail.ac4.yahoo.com> 45?5?C Ren? J. --- On Wed, 7/2/08, Carol Fields wrote: From: Carol Fields Subject: [Histonet] Water bath temps To: histonet@lists.utsouthwestern.edu Date: Wednesday, July 2, 2008, 9:45 AM Hi Netters, We received a recommendation.. for not having water bath temp ranges (QC chart) on our last CAP inspection. I thought this was taken off the list? What range do you all have on your QC sheets for your water bath temps? Thanks in advance, Carole Fields Northside Hospital Atlanta, GA CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Carol.Fields <@t> Northside.com Wed Jul 2 11:07:44 2008 From: Carol.Fields <@t> Northside.com (Carol Fields) Date: Wed Jul 2 11:07:49 2008 Subject: [Histonet] Water bath temps.... Message-ID: <038809F21D1F9040A1FEAD72A7E2C6B509724645@NSMXMS04.northside.local> Thanks to all of you for the info on the water baths. Carole Fields Northside Hospital Atlanta, GA CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. From christiegowan <@t> msn.com Wed Jul 2 11:09:16 2008 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Wed Jul 2 11:09:27 2008 Subject: [Histonet] Water bath temps Message-ID: Carol, Our QC charts for waterbath temps read 42 - 44ºC +/- 2º. From JWeems <@t> sjha.org Wed Jul 2 11:53:37 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Jul 2 11:53:41 2008 Subject: [Histonet] Water bath temps.... In-Reply-To: <038809F21D1F9040A1FEAD72A7E2C6B509724645@NSMXMS04.northside.local> References: <038809F21D1F9040A1FEAD72A7E2C6B509724645@NSMXMS04.northside.local> Message-ID: <982A0A9461F9BF438C7B19A6E425A383372ADB@ITSSSXM01V6.one.ads.che.org> I thought we don't have to record these anymore? Has it been added and I slept through it? j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Fields Sent: Wednesday, July 02, 2008 12:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water bath temps.... Thanks to all of you for the info on the water baths. Carole Fields Northside Hospital Atlanta, GA CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From Charlene.Henry <@t> STJUDE.ORG Wed Jul 2 12:08:01 2008 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Wed Jul 2 12:08:07 2008 Subject: [Histonet] Water bath temps. . In-Reply-To: <410640.51877.qm@web65712.mail.ac4.yahoo.com> Message-ID: <03E1F5968F60C5448635D49D38B283ED01460FD8FA@SJMEMXMBS11.stjude.sjcrh.local> Rene, It is not a CAP requirement to have a QC Temperature chart for water-baths; however you must have a temperature range on any QC log that captures the temperature of an instrument. Our temperature range is 40-50?C. Charlene -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, July 02, 2008 10:55 AM To: histonet@lists.utsouthwestern.edu; Carol Fields Subject: Re: [Histonet] Water bath temps. . 45?5?C Ren? J. --- On Wed, 7/2/08, Carol Fields wrote: From: Carol Fields Subject: [Histonet] Water bath temps To: histonet@lists.utsouthwestern.edu Date: Wednesday, July 2, 2008, 9:45 AM Hi Netters, We received a recommendation.. for not having water bath temp ranges (QC chart) on our last CAP inspection. I thought this was taken off the list? What range do you all have on your QC sheets for your water bath temps? Thanks in advance, Carole Fields Northside Hospital Atlanta, GA CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Shirley_PHUA <@t> hsa.gov.sg Wed Jul 2 13:02:53 2008 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Wed Jul 2 13:03:52 2008 Subject: [Histonet] Shirley Phua is out-of-office ... Message-ID: I will be out of the office from 02-07-2008 to 03-07-2008. I'll be away on 02 July 2008 afternoon. Pathologists: I will process your requests when I return. If urgent, please forward your email to Henry_Kyaw@hsa.gov.sg From eca9 <@t> georgetown.edu Wed Jul 2 13:58:02 2008 From: eca9 <@t> georgetown.edu (Eva CA Permaul) Date: Wed Jul 2 13:58:05 2008 Subject: [Histonet] her2/neu antibody? Message-ID: <183c8c186785.186785183c8c@imap.georgetown.edu> Hello, I want to try two different her2/neu antibodies on FFPE tissue. Has anyone used Zymed TAB250 cat.no. 28-0003Z or Cell signaling cat.no. 2242? If yes, which conditions did you use (ie. antigen retrieval, concentration, incubation time and detection method)? Thanks for your help. Eva Georgetown University From rjbuesa <@t> yahoo.com Wed Jul 2 14:56:17 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jul 2 14:56:21 2008 Subject: [Histonet] Water bath temps. . In-Reply-To: <03E1F5968F60C5448635D49D38B283ED01460FD8FA@SJMEMXMBS11.stjude.sjcrh.local> Message-ID: <518051.11730.qm@web65702.mail.ac4.yahoo.com> I was merely answering a question giving the range I used to keep. Retired as I am now CAP regulations are of minimal significance for me. By the way, 40-50? is EXACTLY the same as 45?5?C Ren? J. --- On Wed, 7/2/08, Henry, Charlene wrote: From: Henry, Charlene Subject: RE: [Histonet] Water bath temps. . To: "'rjbuesa@yahoo.com'" , "histonet@lists.utsouthwestern.edu" , "Carol Fields" Date: Wednesday, July 2, 2008, 1:08 PM Rene, It is not a CAP requirement to have a QC Temperature chart for water-baths; however you must have a temperature range on any QC log that captures the temperature of an instrument. Our temperature range is 40-50?C. Charlene -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, July 02, 2008 10:55 AM To: histonet@lists.utsouthwestern.edu; Carol Fields Subject: Re: [Histonet] Water bath temps. . 45?5?C Ren? J. --- On Wed, 7/2/08, Carol Fields wrote: From: Carol Fields Subject: [Histonet] Water bath temps To: histonet@lists.utsouthwestern.edu Date: Wednesday, July 2, 2008, 9:45 AM Hi Netters, We received a recommendation.. for not having water bath temp ranges (QC chart) on our last CAP inspection. I thought this was taken off the list? What range do you all have on your QC sheets for your water bath temps? Thanks in advance, Carole Fields Northside Hospital Atlanta, GA CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Wed Jul 2 15:21:02 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Jul 2 15:21:39 2008 Subject: [Histonet] pdr hematoxylin Message-ID: <000a01c8dc81$38684ab0$6401a8c0@Patsyoffice> Is there a shortage, got a desperate call from someone who cannot buy powered hematoxylin? Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From Vickroy.Jim <@t> mhsil.com Wed Jul 2 15:26:18 2008 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Wed Jul 2 15:30:57 2008 Subject: [Histonet] need link to latest histotech salary survey Message-ID: <24A4826E8EF0964D86BC5317306F58A50686C88394@mmc-mail.ad.mhsil.com> Does anyone know where I can easily get the latest breakdown of histotech salaries? I think ASCP publishes a salary survey every so often. I need information regarding ranges for histotechnicians, histotechnologists, and histology supervisors. I am also interested if anyone can clarify job desciption differences between a technical anatomical pathology manager verses a histology supervisor. Thanks for your help. Jim Vickroy BS, HT(ASCP) Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center 217-788-4046 vickroy.jim@mhsil.com This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From r.leonard <@t> gipath.com Wed Jul 2 15:56:44 2008 From: r.leonard <@t> gipath.com (Rob Leonard) Date: Wed Jul 2 16:01:07 2008 Subject: [Histonet] pdr hematoxylin In-Reply-To: <000a01c8dc81$38684ab0$6401a8c0@Patsyoffice> Message-ID: <200807022100.m62L0neR028165@mail175c2.megamailservers.com> It is my understanding there is a US shortage. Something about Mexican gov't scaling back the number of trees processed. Therefore, shortage here. Lots of my vendors have been pushing the synthetics as a result. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Wednesday, July 02, 2008 3:21 PM To: 'Histonet' Subject: [Histonet] pdr hematoxylin Is there a shortage, got a desperate call from someone who cannot buy powered hematoxylin? Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jryan <@t> sleh.com Wed Jul 2 16:29:25 2008 From: jryan <@t> sleh.com (Ryan, John P.) Date: Wed Jul 2 16:29:33 2008 Subject: [Histonet] Histology job opportunity in Houston Message-ID: St. Luke's Episcopal Hospital, Department of Pathology in Houston, Texas has an opening on the night shift for a Histology technician/technologist. The night shift position will be responsible for embedding, microtomy, routine and some special staining. The goal of the laboratory is to have all of the H&E slides completed by 8:00 AM on a daily basis. You will be working as part of a team with 4 other experienced technicians and technologists to handle the approximately 400-500 blocks per night. Salary will be based on experience but with shift differential will exceed $40,000/year. If interested please contact John Ryan at 832-355-2643 jryan@sleh.com or fill out an application at www.giantcareers.com . St. Luke's Episcopal Hospital Assistant Administrative Director Pathology 832-355-2643 phone 832-355-4232 fax jryan@sleh.com +++++CONFIDENTIALITY NOTICE+++++ The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. From ht.ascp <@t> yahoo.com Wed Jul 2 18:07:33 2008 From: ht.ascp <@t> yahoo.com (Jon Google) Date: Wed Jul 2 18:07:37 2008 Subject: [Histonet] Water bath temps. . In-Reply-To: <518051.11730.qm@web65702.mail.ac4.yahoo.com> Message-ID: <813328.77447.qm@web59907.mail.ac4.yahoo.com> Well, not EXACTLY the same.? 45 means that is the target temperature.? While 40-50 means that anywhere in the range is ok.? Right? --- On Wed, 7/2/08, Rene J Buesa wrote: From: Rene J Buesa Subject: RE: [Histonet] Water bath temps. . To: "histonet@lists.utsouthwestern.edu" <>, "Carol Fields" , "Henry, Charlene" Date: Wednesday, July 2, 2008, 7:56 PM I was merely answering a question giving the range I used to keep. Retired as I am now CAP regulations are of minimal significance for me. By the way, 40-50? is EXACTLY the same as 45?5?C Ren? J. --- On Wed, 7/2/08, Henry, Charlene wrote: From: Henry, Charlene Subject: RE: [Histonet] Water bath temps. . To: "'rjbuesa@yahoo.com'" , "histonet@lists.utsouthwestern.edu" , "Carol Fields" Date: Wednesday, July 2, 2008, 1:08 PM Rene, It is not a CAP requirement to have a QC Temperature chart for water-baths; however you must have a temperature range on any QC log that captures the temperature of an instrument. Our temperature range is 40-50?C. Charlene -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, July 02, 2008 10:55 AM To: histonet@lists.utsouthwestern.edu; Carol Fields Subject: Re: [Histonet] Water bath temps. . 45?5?C Ren? J. --- On Wed, 7/2/08, Carol Fields wrote: From: Carol Fields Subject: [Histonet] Water bath temps To: histonet@lists.utsouthwestern.edu Date: Wednesday, July 2, 2008, 9:45 AM Hi Netters, We received a recommendation.. for not having water bath temp ranges (QC chart) on our last CAP inspection. I thought this was taken off the list? What range do you all have on your QC sheets for your water bath temps? Thanks in advance, Carole Fields Northside Hospital Atlanta, GA CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Wed Jul 2 20:17:11 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Jul 2 20:17:08 2008 Subject: [Histonet] History of Histology Message-ID: <000801c8dcaa$85ed4690$0302a8c0@yourxhtr8hvc4p> Happy Wednesday (or Thursday) Netters I'm in the process of a small project and need to see if you know a book or website that talks about early histology procedures. Who was the first one to use a fixative, dehydrant, clearing agent and infiltration wax? How we developed from jars to automatic processors, etc. My wrists are sore from surfing. I Googled up to 2,000,000 hits. I think I've developed carpal tunnel syndrome. My fingers are numb. Thanks in advacne (see) JTT From Barry.R.Rittman <@t> uth.tmc.edu Wed Jul 2 20:47:50 2008 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Wed Jul 2 20:47:52 2008 Subject: [Histonet] History of Histology References: <000801c8dcaa$85ed4690$0302a8c0@yourxhtr8hvc4p> Message-ID: Joe "A History of Microtechnique" Brian Bracegirdle. Second Edition, 1987. Science Heritage Limited Publishers, Lincolnwood Illinois 60646. ISBN 0-940095-00-9 "The Microscopists Vade Mecum -Bolles Lee". Gattenby and Painter editors. 1937 Churchill, London "Physiological Histology. Methods and Theory" Gustav Mann, 1902 Oxford Clarendon Press. "Encyclopedia der Mikroskopische Technik" - 3 volumes. Krause 1921- but in German. Urban and Schwartzenberg, Berlin. I have these if you need me to look up any items. Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Joe Nocito Sent: Wed 7/2/2008 8:17 PM To: histonet Subject: [Histonet] History of Histology Happy Wednesday (or Thursday) Netters I'm in the process of a small project and need to see if you know a book or website that talks about early histology procedures. Who was the first one to use a fixative, dehydrant, clearing agent and infiltration wax? How we developed from jars to automatic processors, etc. My wrists are sore from surfing. I Googled up to 2,000,000 hits. I think I've developed carpal tunnel syndrome. My fingers are numb. Thanks in advacne (see) JTT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pieronelva01 <@t> bigpond.com Wed Jul 2 21:55:13 2008 From: pieronelva01 <@t> bigpond.com (Piero Nelva) Date: Wed Jul 2 21:55:19 2008 Subject: [Histonet] History of Histology References: <000801c8dcaa$85ed4690$0302a8c0@yourxhtr8hvc4p> Message-ID: <003801c8dcb8$3773faa0$a473be7c@pentium4> I can also recommend the volume by Bracegirdle "History of Microtechnique". Everything you ever wanted to know about microtomes and related equipment. Piero ----- Original Message ----- From: "Rittman, Barry R" To: "Joe Nocito" ; "histonet" Sent: Thursday, July 03, 2008 11:47 AM Subject: RE: [Histonet] History of Histology Joe "A History of Microtechnique" Brian Bracegirdle. Second Edition, 1987. Science Heritage Limited Publishers, Lincolnwood Illinois 60646. ISBN 0-940095-00-9 "The Microscopists Vade Mecum -Bolles Lee". Gattenby and Painter editors. 1937 Churchill, London "Physiological Histology. Methods and Theory" Gustav Mann, 1902 Oxford Clarendon Press. "Encyclopedia der Mikroskopische Technik" - 3 volumes. Krause 1921- but in German. Urban and Schwartzenberg, Berlin. I have these if you need me to look up any items. Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Joe Nocito Sent: Wed 7/2/2008 8:17 PM To: histonet Subject: [Histonet] History of Histology Happy Wednesday (or Thursday) Netters I'm in the process of a small project and need to see if you know a book or website that talks about early histology procedures. Who was the first one to use a fixative, dehydrant, clearing agent and infiltration wax? How we developed from jars to automatic processors, etc. My wrists are sore from surfing. I Googled up to 2,000,000 hits. I think I've developed carpal tunnel syndrome. My fingers are numb. Thanks in advacne (see) JTT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG. Version: 8.0.134 / Virus Database: 270.4.4/1531 - Release Date: 7/2/2008 7:02 PM From alabamabuddyblue <@t> yahoo.com Wed Jul 2 22:01:49 2008 From: alabamabuddyblue <@t> yahoo.com (Toni Drinkard) Date: Wed Jul 2 22:01:53 2008 Subject: [Histonet] CAP/CLIA regulations Message-ID: <286639.36737.qm@web37606.mail.mud.yahoo.com> I would like to know if the regulations differ from state to state regarding gross tissue exam. Do you have to have a degree or is there a grandfather clause of some sort that would cover a non-registered histotech that is now learning to gross? I was under the impression that a degree is now required. I would like to know Mississippi's regulations. Thanks T. Mosley From Traczyk7 <@t> aol.com Wed Jul 2 22:32:56 2008 From: Traczyk7 <@t> aol.com (Traczyk7@aol.com) Date: Wed Jul 2 22:33:06 2008 Subject: [Histonet] Water Bath Temperature Message-ID: For what it's worth. I seem to remember the old HT exam question going something like this... What is the proper water bath temperature? Answer: 10 to 12 degress below the melting point of the paraffin being used for embedding. Covers all bases and would nicely allow for an acceptable range to be posted on temp. chart. As for the requirement to still have that chart, don't know, but what's the harm? Dorothy **************Gas prices getting you down? Search AOL Autos for fuel-efficient used cars. (http://autos.aol.com/used?ncid=aolaut00050000000007) From freckles9660 <@t> yahoo.com Wed Jul 2 23:06:06 2008 From: freckles9660 <@t> yahoo.com (Karla Arrington) Date: Wed Jul 2 23:06:09 2008 Subject: [Histonet] Giemsa problems Message-ID: <417328.81696.qm@web32504.mail.mud.yahoo.com> Microscopic exam of a bone marrow aspirate stained with Giemsa shows blue-gray erythrocytes and blue leukocytes. Is this caused by the solution too alkaline, too acid, from an anemic patient or just fine?? Also can you prepare stock neutralized formalin by storing the solution over a layer of calcium carbonate, when the solution withdrawn from this stock container will it be acidic, alkaline, neutral or what?? ? Thanks! Karla Arrington freckles9660@yahoo.com From Ari.Liebkowsky <@t> charite.de Thu Jul 3 05:25:58 2008 From: Ari.Liebkowsky <@t> charite.de (Liebkowsky, James Mateo Ari) Date: Thu Jul 3 05:26:07 2008 Subject: [Histonet] Paraffin blocks (reprise) Message-ID: <75713DE92671A742B8895F709A3129CDD2EA13@EXCHANGE02.charite.de> Hi Rene, Thanks for your response. Well, the blocks are already being used and the collection hasn't even been fully compiled yet, so I guess we can say the work has a purpose. My first idea was also to run random tests, however, I'm not a histologist but an administrator. Scientists who have used the blocks insist that the blocks are still usable, even if it's not as good as a fresh sample - they say it depends on the how good the antibody used is and which question is being asked. So I will go with their judgement. Can I ask why you consider fumigation to be the best option? Kr, James =============================== James Ari Liebkowsky SFB 665 Coordinator PA to Professor Nitsch Institute for Cell Biology and Neurobiology Center for Anatomy Charit? - Universit?tsmedizin Berlin Tel.: +49 30 450 528 072 Fax.: +40 30 450 528 902 E-mail: ari.liebkowsky@charite.de www.charite.de/sfb665 www.charite.de/anatomie/nitsch From SharonC <@t> celligent.net Thu Jul 3 07:20:21 2008 From: SharonC <@t> celligent.net (Sharon Campbell) Date: Thu Jul 3 07:16:19 2008 Subject: [Histonet] Penfix in processing Message-ID: Hello histonetters, Here is Fridays question on Thursday: Does anyone use Penfix for fixation and on the processor? Also, If a breast was originally fixed in 10% NBF for the required 6 hours and then put in penfix for a few hours up to overnight will this be a problem for the Her2neu testing? Thank you in advance for your help, Sharon Campbell, HTL(ASCP)CM, BSBM Histology Supervisor Celligent Diagnostics, LLC Formerly Pathology Associates Services 101 East W.T. Harris Blvd, Suite 1212 Charlotte, NC 28262 (704) 549-8444 x100 sharonc@celligent.net From rjbuesa <@t> yahoo.com Thu Jul 3 07:29:30 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jul 3 07:29:34 2008 Subject: [Histonet] History of Histology In-Reply-To: <000801c8dcaa$85ed4690$0302a8c0@yourxhtr8hvc4p> Message-ID: <472290.34689.qm@web65709.mail.ac4.yahoo.com> Joe: Under separate cover I am sending an article I wrote on a similar context with answers to some of your questions. There is also en article in the JOH by Titford on the subject. Ren? J. --- On Wed, 7/2/08, Joe Nocito wrote: From: Joe Nocito Subject: [Histonet] History of Histology To: "histonet" Date: Wednesday, July 2, 2008, 9:17 PM Happy Wednesday (or Thursday) Netters I'm in the process of a small project and need to see if you know a book or website that talks about early histology procedures. Who was the first one to use a fixative, dehydrant, clearing agent and infiltration wax? How we developed from jars to automatic processors, etc. My wrists are sore from surfing. I Googled up to 2,000,000 hits. I think I've developed carpal tunnel syndrome. My fingers are numb. Thanks in advacne (see) JTT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Jul 3 07:37:39 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jul 3 07:37:42 2008 Subject: [Histonet] Giemsa problems In-Reply-To: <417328.81696.qm@web32504.mail.mud.yahoo.com> Message-ID: <323399.56844.qm@web65708.mail.ac4.yahoo.com> The dyes in Giemsa are essentially pH indicators, so a bluish hue is due to an alkaline? (high pH) environment. If you keep formalin over a layer of calcium carbonate that will prevent the formation of formic acid (oxidation of the formaldehyde), BUT once you take the formalin out of that container and use it to fix tissues, the neutralizing effect will have disappeared and the acidification of the formalin is again an issue. Ren? J. --- On Thu, 7/3/08, Karla Arrington wrote: ? From rjbuesa <@t> yahoo.com Thu Jul 3 07:45:16 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jul 3 07:45:21 2008 Subject: [Histonet] Penfix in processing In-Reply-To: Message-ID: <660978.86922.qm@web65716.mail.ac4.yahoo.com> It will most definitely be a problem during any CAP inspection because the FDA procedure is approved for fixation with formalin exclusively. Also the alcohols contained in Pen Fix (methanol, ethanol and 2-propanol) will probably affect the antigen preservation in a specimen that with 6 hours fixation is only 28% crosslinked with the formaldehyde. It is much better to leave breast specimens in 10% NBF especially if you have the opportunity of leaving them overnight. Ren? J. --- On Thu, 7/3/08, Sharon Campbell wrote: ? From jryan <@t> sleh.com Thu Jul 3 09:16:33 2008 From: jryan <@t> sleh.com (Ryan, John P.) Date: Thu Jul 3 09:16:53 2008 Subject: [Histonet] History of Histology In-Reply-To: <000801c8dcaa$85ed4690$0302a8c0@yourxhtr8hvc4p> References: <000801c8dcaa$85ed4690$0302a8c0@yourxhtr8hvc4p> Message-ID: Joe, A book titled "A History of Microtechnique" written by Brian Bracegirdle (one of the Science Heritage Collection of books) states in the foreward that it was written to "traces the origins and development of the manipulations needed to prepare specimens for examination by the light microscope. It also has an extensive bibliography on the type of information you are looking for. I have a copy if you are unable to locate one. John Assistant Administrative Director Pathology 832-355-2643 phone 832-355-4232 fax jryan@sleh.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, July 02, 2008 8:17 PM To: histonet Subject: [Histonet] History of Histology Happy Wednesday (or Thursday) Netters I'm in the process of a small project and need to see if you know a book or website that talks about early histology procedures. Who was the first one to use a fixative, dehydrant, clearing agent and infiltration wax? How we developed from jars to automatic processors, etc. My wrists are sore from surfing. I Googled up to 2,000,000 hits. I think I've developed carpal tunnel syndrome. My fingers are numb. Thanks in advacne (see) JTT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet +++++CONFIDENTIALITY NOTICE+++++ The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. From froyer <@t> bitstream.net Thu Jul 3 09:33:57 2008 From: froyer <@t> bitstream.net (Ford Royer) Date: Thu Jul 3 09:34:13 2008 Subject: [Histonet] History of Histology In-Reply-To: References: <000801c8dcaa$85ed4690$0302a8c0@yourxhtr8hvc4p> Message-ID: <004101c8dd19$d409ebc0$7701a80a@Ford> I always thought that Dr. Jim McCormick & Freida Carson invented histology.... ;-) Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL:? 612-839-1046 Phone:? 763-542-8725 Fax:? 763-546-4830 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ryan, John P. Sent: Thursday, July 03, 2008 9:17 AM To: 'Joe Nocito'; histonet Subject: RE: [Histonet] History of Histology Joe, A book titled "A History of Microtechnique" written by Brian Bracegirdle (one of the Science Heritage Collection of books) states in the foreward that it was written to "traces the origins and development of the manipulations needed to prepare specimens for examination by the light microscope. It also has an extensive bibliography on the type of information you are looking for. I have a copy if you are unable to locate one. John Assistant Administrative Director Pathology 832-355-2643 phone 832-355-4232 fax jryan@sleh.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Wednesday, July 02, 2008 8:17 PM To: histonet Subject: [Histonet] History of Histology Happy Wednesday (or Thursday) Netters I'm in the process of a small project and need to see if you know a book or website that talks about early histology procedures. Who was the first one to use a fixative, dehydrant, clearing agent and infiltration wax? How we developed from jars to automatic processors, etc. My wrists are sore from surfing. I Googled up to 2,000,000 hits. I think I've developed carpal tunnel syndrome. My fingers are numb. Thanks in advacne (see) JTT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet +++++CONFIDENTIALITY NOTICE+++++ The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From freckles9660 <@t> yahoo.com Thu Jul 3 10:05:58 2008 From: freckles9660 <@t> yahoo.com (Karla Arrington) Date: Thu Jul 3 10:06:02 2008 Subject: [Histonet] troubleshooting Message-ID: <184710.60146.qm@web32506.mail.mud.yahoo.com> 2 questions for you all. What causes tissue sections after H&E stained fixed in formlain, to have nuclear bubbling? Is it under fixation, prolonged fixation? Also what is the best way to store for a short time, fresh, unfixed tissue either in a saline moistened gauze and refrigerated or just placing it in physiological saline at room temperature.? I am troubleshooting some problems. Karla Arrington freckles9660@yahoo.com From EdieL <@t> fmchealth.org Thu Jul 3 10:29:54 2008 From: EdieL <@t> fmchealth.org (Edie Lehman) Date: Thu Jul 3 10:30:00 2008 Subject: [Histonet] PAS stain decolorizer? Message-ID: <3C25F9EF8E8DF84DBBB1884C297E8AF4037907DE@ex03.fmchealth.org> We have a section that was incorrectly stained with PAS and no tissue left to recut. We need to do an H.Pylori stain on the slide. Any thoughts on how to destain the PAS? Tech tried acid alcohol to no avail. Thanks Edie Lehman MT(ASCP) Anatomical Pathology Supervisor Fairfield Medical Center "Confidentiality Notice: This e-mail message, including any attachments is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review; use; disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message." From Carol.Fields <@t> Northside.com Thu Jul 3 10:46:13 2008 From: Carol.Fields <@t> Northside.com (Carol Fields) Date: Thu Jul 3 10:46:19 2008 Subject: [Histonet] PAS stain decolorizer? In-Reply-To: <3C25F9EF8E8DF84DBBB1884C297E8AF4037907DE@ex03.fmchealth.org> References: <3C25F9EF8E8DF84DBBB1884C297E8AF4037907DE@ex03.fmchealth.org> Message-ID: <038809F21D1F9040A1FEAD72A7E2C6B509724651@NSMXMS04.northside.local> Anatech or one of the companies make a powder that removes PAS from your hands and clothes. I mixed a small amount of the powder in a coplan jar with dist H20 and put the slides in there for a few minutes and it took the Schiff's out. Then you wash them well and stain. Carole Fields Northside Hospital Atlanta, GA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edie Lehman Sent: Thursday, July 03, 2008 11:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAS stain decolorizer? We have a section that was incorrectly stained with PAS and no tissue left to recut. We need to do an H.Pylori stain on the slide. Any thoughts on how to destain the PAS? Tech tried acid alcohol to no avail. Thanks Edie Lehman MT(ASCP) Anatomical Pathology Supervisor Fairfield Medical Center "Confidentiality Notice: This e-mail message, including any attachments is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review; use; disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. From rjbuesa <@t> yahoo.com Thu Jul 3 10:46:48 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jul 3 10:46:53 2008 Subject: [Histonet] troubleshooting In-Reply-To: <184710.60146.qm@web32506.mail.mud.yahoo.com> Message-ID: <139328.88907.qm@web65709.mail.ac4.yahoo.com> Usually it is caused by drying the sections before being completely drained and at high temperature (I am sending you an article on the subject under separate cover). Keeping a "fresh" tissue in any of the ways you describe will lead to putrefaction and complete loss of cellular detail.The best way will be to snap freezing it. Ren? J. --- On Thu, 7/3/08, Karla Arrington wrote: From: Karla Arrington Subject: [Histonet] troubleshooting To: histonet@lists.utsouthwestern.edu Date: Thursday, July 3, 2008, 11:05 AM 2 questions for you all. What causes tissue sections after H&E stained fixed in formlain, to have nuclear bubbling? Is it under fixation, prolonged fixation? Also what is the best way to store for a short time, fresh, unfixed tissue either in a saline moistened gauze and refrigerated or just placing it in physiological saline at room temperature.? I am troubleshooting some problems. Karla Arrington freckles9660@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Jul 3 10:51:32 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jul 3 10:52:01 2008 Subject: [Histonet] PAS stain decolorizer? In-Reply-To: <3C25F9EF8E8DF84DBBB1884C297E8AF4037907DE@ex03.fmchealth.org> Message-ID: <583611.34808.qm@web65701.mail.ac4.yahoo.com> You can destain PAS with a very very very?diluted solution of Clorox. Ren? J. --- On Thu, 7/3/08, Edie Lehman wrote: From asmith <@t> mail.barry.edu Thu Jul 3 10:53:13 2008 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Thu Jul 3 10:54:18 2008 Subject: [Histonet] PAS stain decolorizer? In-Reply-To: <038809F21D1F9040A1FEAD72A7E2C6B509724651@NSMXMS04.northside.local> References: <3C25F9EF8E8DF84DBBB1884C297E8AF4037907DE@ex03.fmchealth.org> <038809F21D1F9040A1FEAD72A7E2C6B509724651@NSMXMS04.northside.local> Message-ID: My research assistant, Isaac Glickfield, was intrigued by the problem. He found that overnight immersion in concentrated (i.e., 28%) ammonium hydroxide removes the Schiff reagent and restores the original aldehydes in the tissue. Allen A. Smith Barry University School of Podiatric Medicine Miami Shores, Florida -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Fields Sent: Thursday, July 03, 2008 11:46 AM To: Edie Lehman Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PAS stain decolorizer? Anatech or one of the companies make a powder that removes PAS from your hands and clothes. I mixed a small amount of the powder in a coplan jar with dist H20 and put the slides in there for a few minutes and it took the Schiff's out. Then you wash them well and stain. Carole Fields Northside Hospital Atlanta, GA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edie Lehman Sent: Thursday, July 03, 2008 11:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAS stain decolorizer? We have a section that was incorrectly stained with PAS and no tissue left to recut. We need to do an H.Pylori stain on the slide. Any thoughts on how to destain the PAS? Tech tried acid alcohol to no avail. Thanks Edie Lehman MT(ASCP) Anatomical Pathology Supervisor Fairfield Medical Center "Confidentiality Notice: This e-mail message, including any attachments is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review; use; disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Thu Jul 3 11:19:09 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Jul 3 11:19:14 2008 Subject: [Histonet] PAS stain decolorizer? In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3882@IS-E2K3.grhs.net> This brings up a point of interest. Would you not need to treat your control tissue that you are going to now do a new stain in the same manner to assure stain quality. Just wondering? Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith, Allen Sent: Thursday, July 03, 2008 10:53 AM To: 'Carol Fields' Cc: 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] PAS stain decolorizer? My research assistant, Isaac Glickfield, was intrigued by the problem. He found that overnight immersion in concentrated (i.e., 28%) ammonium hydroxide removes the Schiff reagent and restores the original aldehydes in the tissue. Allen A. Smith Barry University School of Podiatric Medicine Miami Shores, Florida -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carol Fields Sent: Thursday, July 03, 2008 11:46 AM To: Edie Lehman Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PAS stain decolorizer? Anatech or one of the companies make a powder that removes PAS from your hands and clothes. I mixed a small amount of the powder in a coplan jar with dist H20 and put the slides in there for a few minutes and it took the Schiff's out. Then you wash them well and stain. Carole Fields Northside Hospital Atlanta, GA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edie Lehman Sent: Thursday, July 03, 2008 11:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAS stain decolorizer? We have a section that was incorrectly stained with PAS and no tissue left to recut. We need to do an H.Pylori stain on the slide. Any thoughts on how to destain the PAS? Tech tried acid alcohol to no avail. Thanks Edie Lehman MT(ASCP) Anatomical Pathology Supervisor Fairfield Medical Center "Confidentiality Notice: This e-mail message, including any attachments is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review; use; disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From twheelock <@t> mclean.harvard.edu Thu Jul 3 13:07:01 2008 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Thu Jul 3 12:08:25 2008 Subject: [Histonet] PALE MYELIN STAIN Message-ID: <486D1545.1060209@mclean.harvard.edu> Hi Everyone: Lately, I have been having problems with my Luxol Fast Blue myelin stain coming out too pale. I have gone through each step and can't figure out where the problem originates. I even bought another bottle of the dye itself to no avail. I rechecked and re-made the The Luxol Fast Blue working solution (0.1 gram Luxol Fast Blue and 1 ml of 10% glacial acetic acid in 100 mls of 95% ethanol). I did the same for the differentiator(1% Hydroquinone/5%Sodium Sulfite). That made no difference at all. The sections look pale after I remove the excess stain in 95% before the differentiator, so I assume it is not a mistake making up the later or a problem with the H+ E that I add onto the myelin stain. I adjusted by increasing the incubation time in a 60C oven from 2 to 4 hours. And decreased the time in the differentiator from 2 dips to 1. This helped a lot, but I knew that these changes did not get to the root of the problem. Yesterday, they still came out pale even with these changes in place I keep thinking the the problem is in the working solution but am not sure how. (Fixation, processing and sectioning are the same as they have always been.) I have done this stain for years and never had a problem...until now. Thanks for any suggestions you may have. Tim Wheelock Harvard Brain Tissue Resource Center 203 Mailman Research Center McLean Hospital Belmont MA 02478 Phone: 617-855-3592 Fax: 617-855-3199 The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From jdominguez.vitro <@t> gmail.com Thu Jul 3 12:08:37 2008 From: jdominguez.vitro <@t> gmail.com (Jorge Dominguez) Date: Thu Jul 3 12:08:42 2008 Subject: [Histonet] zinc formalin on ISH Message-ID: <68e1fc010807031008g3ce27dc6r14f08269d8526594@mail.gmail.com> Hi, I was wondering if anyone could offer some advise on zinc formalin. We are currently using 10% NBF on our tissues. We are considering switching to zinc formalin to shorten processing time. Any help would be greatly appreciated. Thanks From liz <@t> premierlab.com Thu Jul 3 12:29:56 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Jul 3 12:30:08 2008 Subject: [Histonet] zinc formalin on ISH In-Reply-To: <68e1fc010807031008g3ce27dc6r14f08269d8526594@mail.gmail.com> References: <68e1fc010807031008g3ce27dc6r14f08269d8526594@mail.gmail.com> Message-ID: Jorge Why would switching from formalin to zinc formalin shorten your processing time? Isn't the fixation time for formalin and zinc formalin the same? My impression is that any formalin based fixative is going to take about 6 hours to adequately fix tissue, if it is shorter than that you will get a combination of formalin and alcohol based fixation (via your processing post fixation). Back in the 80's when we first started using zinc formalin as a replacment to B-5 you needed to fix in zinc formalin for at least 6 hours to obtain the better nuclear detail. The zinc chloride or zinc sulfate that is used in the fixative acts similar to the mercuric chloride in the B-5 fixative by precipitating nuclear protein (both are considered a protein coagulants) therefore the better morphology. The zinc within the fixative also acts to preserve the 3 dimentionial structure of the protein molecule thus inhibiting excessive cross linking of the formalin fixative (which may mask epitopes on the protein molecule). That's why it's a great fixative for both increased nuclear detail and enhanced immunoreactivity. But as far as I am aware fixation time is no different than routine formalin fixation. If someone knows more, please correct me if I'm wrong. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jorge Dominguez Sent: Thursday, July 03, 2008 11:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] zinc formalin on ISH Hi, I was wondering if anyone could offer some advise on zinc formalin. We are currently using 10% NBF on our tissues. We are considering switching to zinc formalin to shorten processing time. Any help would be greatly appreciated. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> Stowers-Institute.org Thu Jul 3 13:43:11 2008 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Thu Jul 3 13:43:41 2008 Subject: [Histonet] RE: PhosphoGuard fixative additive In-Reply-To: <63EA0607835FBA4689CEA9EA8B48269201307359@usctmx1141.merck.com> Message-ID: Brett, I have not tried the PhosphoGuard, but I have had some of our researchers here use a fixative recommended by Ventana for phosphorylated proteins. Below is the information in its entirety (apologies for the length). Introduction The phosphorylation state of serine, threonine and tyrosine residues of regulartory proteins control a wide variety of cell functions including cell cycle, growth, apoptosis and signal transduction. Examining the phosphorylation and/or dephosphorylation state of these proteins in fixed tissues may provide insights into cell's response to changes in internal and external environments as well as their impact on disease states. Due to the presence of cellular phosphatases, however, the half-life of many phosphorylated proteins is short and may be underrepresented if tissue specimens are not fixed immediately upon excision in a fixative designed to inactivate cellular phosphatases as well as fix the tissue. Ventana Fixative is a multi-component, formaldehyde based fixative designed to rapidly inactivate phosphatases in cell and tissue preparations. The final cocktail contains sodium fluoride for inhibition of serine and threonine phosphatases, sodium orthovanadate for inhibition of tyrosine phosphatases, sodium molybdate for inhibition of phosphoprotein phosphatases, sodium tartrate for inhibition of acid phosphatases, imidazole for inhibition of alkaline phosphatases, and formalin for rapid cell or tissue fixation. Instructions for Use Just prior to use add 200 ul of Fix Prep 1 and 200 ul of Fix Prep 2 (see formulations below) per 10.0 ml 10% Neutral Buffered Formalin (NBF). Mix well. Tissue specimens should be immersed in a minimum of 15 volumes of Fixative for 24 hours and then transferred to 70% ethanol for transport or storage until processing. Cell suspensions should be pelleted and resuspended in a minimum of 3 mls of Fixative for 4-6 hours, and then transferred to 70% ethanol for transport or storage until processing. Tissues should be placed into Fixative immediately upon excision. Any delay in placing the excised specimen into Ventana Fixative may impact the quality of phosphoprotein preservation. Please carefully read the MSDS instructions and all associated product information provided by the product vendor including safety precautions associated with each raw material and their appropriate use. Latex (or equivalent) gloves, safety glasses, and other appropriate personal protection should be worn when handling the reagents. Fixative Formulations Fix Prep I - 0.5M Sodium Fluoride - 2.1g Sodium Fluoride (Sigma Cat. No. S7920) in 100 ml deionized water Fix Prep 2 - 50% Phosphatase Cocktail 2 - Dilute Phosphatase Cocktail 2 (Sigma Ca. No. P5726) 1:1 in deionized water Store both at 2-8 degrees C (Do not freeze) I hope this provides an alternative to try for those who would rather make their own material over buying commercially prepared reagents. Best wishes, Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 -----Original Message----- From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Connolly, Brett M Sent: Wednesday, July 02, 2008 8:46 AM To: histonet@lists.utsouthwestern.edu; ihcrg@googlegroups.com Subject: [IHCRG] PhosphoGuard fixative additive In the June 2008 issue of CAP Today, I ran across a fixative additive called PhosphoGuard from Targeted Molecular Technologies. They claim it prevents degradation of phosphorylated and activated proteins allowing for better IHC detection (stronger/more abundant signal) by better inactivation of phosphatases compared to plain 10% NBF. Apparently you just add some to your formalin. You can check it out further on their website. http://www.tmdlab.com/technologies_PHOSPHO.htm Just wondering if any of you have tried it? Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Research Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com From rjbuesa <@t> yahoo.com Thu Jul 3 14:18:22 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jul 3 14:18:27 2008 Subject: [Histonet] PALE MYELIN STAIN In-Reply-To: <486D1545.1060209@mclean.harvard.edu> Message-ID: <914296.26426.qm@web65703.mail.ac4.yahoo.com> Have you made any changes (reagents wise) in tissue processing, including fixation? Ren? J. --- On Thu, 7/3/08, Tim Wheelock wrote: From sbeam.ppcs <@t> q.com Thu Jul 3 14:27:34 2008 From: sbeam.ppcs <@t> q.com (Sheree Beam) Date: Thu Jul 3 14:27:40 2008 Subject: [Histonet] (no subject) Message-ID: Greetings, I received Masson's trichrome stains of arteries and adjacent muscle from a histolab. The first time I received them, the entire artery was blue and the adjacent muscle was purple to sometimes red. The smooth muscle in the arteries were completely blue. However the control tissue of kidney which included a small artery stained perfectly. I asked for the tissue to be recut and try again with Masson's. This time the smooth muscle in the artery has some magenta, but in other areas the blue prevailed. More controls were run and all were perfect. The tissues were fixed in 10% neutral buffered formalin for 10 or 30 days as there were 2 groups. We used two different boxes of formalin. After trim, the tissues were placed back into formalin and sent to the histo lab. There was no fixation in ethanol. Are there any explanations out there? Is there anything we can do? I need the trichromes for photomicrographs. Would a different trichrome stain potentially help? All input would be appreciated. Thank you, Sheree Sheree Beam, DVM, MS, ACVP, Diplomate Veterinary Pathologist Preclinical Pathology Consulting Service, LLC From tdobersztyn <@t> chmca.org Thu Jul 3 15:05:29 2008 From: tdobersztyn <@t> chmca.org (tdobersztyn@chmca.org) Date: Thu Jul 3 15:05:33 2008 Subject: [Histonet] Theresa R. Dobersztyn/CHMCA is out of the office. Message-ID: I will be out of the office starting 07/03/2008 and will not return until 07/07/2008. I will respond to your message when I return. Akron Children's Hospital - Proud winner of the NorthCoast 99 "Best Workplace" award! ************************************************************************* This electronic mail transmission, including any attached files, may contain confidential and/or privileged information for the sole use of the intended recipient(s). It is not intended for transmission to, or receipt by, any unauthorized parties. Any review, use, distribution, dissemination, downloading, copying or disclosure by others is strictly prohibited. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that you have received this transmission in error. If you have received this transmission in error, please delete it, as well as any copies, from your system without copying it, and notify the sender by reply e-mail. From mickie25 <@t> netzero.net Thu Jul 3 16:55:14 2008 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Thu Jul 3 16:55:26 2008 Subject: [Histonet] PALE MYELIN STAIN In-Reply-To: <486D1545.1060209@mclean.harvard.edu> References: <486D1545.1060209@mclean.harvard.edu> Message-ID: Tim, It might be prudent to try the stain again on a block processed at an earlier time when the stain was working and see if the results have remained the same. If it is pale now but wasn't then, then it might be the dye powder at issue. Has your water changed? Good Luck. Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock Sent: Thursday, July 03, 2008 11:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PALE MYELIN STAIN Hi Everyone: Lately, I have been having problems with my Luxol Fast Blue myelin stain coming out too pale. I have gone through each step and can't figure out where the problem originates. I even bought another bottle of the dye itself to no avail. I rechecked and re-made the The Luxol Fast Blue working solution (0.1 gram Luxol Fast Blue and 1 ml of 10% glacial acetic acid in 100 mls of 95% ethanol). I did the same for the differentiator(1% Hydroquinone/5%Sodium Sulfite). That made no difference at all. The sections look pale after I remove the excess stain in 95% before the differentiator, so I assume it is not a mistake making up the later or a problem with the H+ E that I add onto the myelin stain. I adjusted by increasing the incubation time in a 60C oven from 2 to 4 hours. And decreased the time in the differentiator from 2 dips to 1. This helped a lot, but I knew that these changes did not get to the root of the problem. Yesterday, they still came out pale even with these changes in place I keep thinking the the problem is in the working solution but am not sure how. (Fixation, processing and sectioning are the same as they have always been.) I have done this stain for years and never had a problem...until now. Thanks for any suggestions you may have. Tim Wheelock Harvard Brain Tissue Resource Center 203 Mailman Research Center McLean Hospital Belmont MA 02478 Phone: 617-855-3592 Fax: 617-855-3199 The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG. Version: 8.0.134 / Virus Database: 270.4.3/1529 - Release Date: 7/1/2008 7:23 PM From rjbuesa <@t> yahoo.com Thu Jul 3 17:02:52 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jul 3 17:02:56 2008 Subject: [Histonet] (no subject) In-Reply-To: Message-ID: <246782.97893.qm@web65708.mail.ac4.yahoo.com> Try a different histotech! Ren? J. --- On Thu, 7/3/08, Sheree Beam wrote: ? From AnthonyH <@t> chw.edu.au Thu Jul 3 18:01:54 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Jul 3 18:02:02 2008 Subject: [Histonet] (no subject) In-Reply-To: Message-ID: Sheree, Masson's stain is quite capricious. Unlike the van Gieson stains, over differentiation of the red muscle stain is common. The use of mordants can often help. Try placing hydrated slides for 30min in Bouins solution (or aqueous saturated picric acid) at 60oC prior to staining. With the use of the blue collagen counterstain, confusion can also occur since under-differentiation of the nuclear stain, Celestine blue-Harris's Hx or some variant of the iron haematoxylin, can render the muscle blue. The blue you are describing could be due to overstaining with the celestine blue-Hx rather than the iron Hx which would look black. Again overstaining with the blue dye of the Massons as well as overdifferentiation of the red dye would render muscle blue. In my experience control sections rarely behave like diagnostic slides with the Massons. Different fixation times and older control sections change in their affinity for the red and blue stins of the Massons. Try a green collagen stain rather than the blue. The photos may look more pleasing or have you considered using a Van Gieson's stain in stead? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheree Beam Sent: Friday, 4 July 2008 5:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Greetings, I received Masson's trichrome stains of arteries and adjacent muscle from a histolab. The first time I received them, the entire artery was blue and the adjacent muscle was purple to sometimes red. The smooth muscle in the arteries were completely blue. However the control tissue of kidney which included a small artery stained perfectly. I asked for the tissue to be recut and try again with Masson's. This time the smooth muscle in the artery has some magenta, but in other areas the blue prevailed. More controls were run and all were perfect. The tissues were fixed in 10% neutral buffered formalin for 10 or 30 days as there were 2 groups. We used two different boxes of formalin. After trim, the tissues were placed back into formalin and sent to the histo lab. There was no fixation in ethanol. Are there any explanations out there? Is there anything we can do? I need the trichromes for photomicrographs. Would a different trichrome stain potentially help? All input would be appreciated. Thank you, Sheree Sheree Beam, DVM, MS, ACVP, Diplomate Veterinary Pathologist Preclinical Pathology Consulting Service, LLC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From Tony_Reilly <@t> health.qld.gov.au Thu Jul 3 18:24:51 2008 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Thu Jul 3 18:25:34 2008 Subject: [Histonet] PAS stain decolorizer? In-Reply-To: <3C25F9EF8E8DF84DBBB1884C297E8AF4037907DE@ex03.fmchealth.org> References: <3C25F9EF8E8DF84DBBB1884C297E8AF4037907DE@ex03.fmchealth.org> Message-ID: <486DEC62.471C.0039.0@health.qld.gov.au> Hi Edie Why risk a decolourisation step which may affect your final staining, there are recognized staining methods for Helicobactor using PAS such as Sayeed's method. It involves staining the gastric mucosa with PAS followed methylene blue giving blue organisms on a pink background. It is the same premise as the Alcian Yellow Toluidine blue procedure, so alternately toluidine blue could be used if that is your preference. regards >>> "Edie Lehman" 4/07/2008 1:29 am >>> We have a section that was incorrectly stained with PAS and no tissue left to recut. We need to do an H.Pylori stain on the slide. Any thoughts on how to destain the PAS? Tech tried acid alcohol to no avail. Thanks Edie Lehman MT(ASCP) Anatomical Pathology Supervisor Fairfield Medical Center "Confidentiality Notice: This e-mail message, including any attachments is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review; use; disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ********************************************************************************** From jmaass <@t> frii.com Thu Jul 3 20:12:46 2008 From: jmaass <@t> frii.com (Janet Maass) Date: Thu Jul 3 20:12:48 2008 Subject: [Histonet] Artery staining References: Message-ID: <002101c8dd73$11db4760$0200a8c0@Janet03b999f> Sheree, Might this be a surgical specimens in which radiofrequency energy was used during surgery? If so you do not always get the staining affects as one might expect. If it is, refer to the article in Histologic, Vol. XXXVII, No. 2, December 2005. Janet Maass From tgenade <@t> gmail.com Fri Jul 4 02:48:00 2008 From: tgenade <@t> gmail.com (Tyrone Genade) Date: Fri Jul 4 02:48:33 2008 Subject: [Histonet] GS isolectin questions Message-ID: Hello, I'm new to the list and the field of histology (busy startinga PhD). I have a question regarding GS BS-I Isolectin B4 (Sigma). This was the lectin used in the article by Kim et al (Neurobiology of Aging 25:491--500, 2004) for selective staining of CNS microglia. They state that it was used at a dilution of 1:50 which is amazingly helpful in light of the fact that no starting concentration is stated. I have been able to contact the corresponding author who gave me the email of the author who did the lectin work but no response... I have purchased 1 mg of the GS isolectin which has now arrived in powdered form. The recommendation by Sigma is to dissovle it in to 0.9% NaCl. Can anyone suggest a starting concentration from which I can make the 1:50 dilutions or better, suggest a good working solution concentration. I will be staining retinal sections of fish, not mammals, so anyone with advise pertinent to fish histology would be appreciated. I understand that as negative control I will have treat control sections with a lactose solution. Can anyone volunteer more information regarding control protocols? Also, I may want to counter stain with an antibody in frozen sections. Would the BSA blocking solution interfer with the lectin binding. We had previously been using tomato lectin with dismal results (it stuck to everything!). Many thanks Tyrone -- Tyrone Genade http://tgenade.freeshell.org email: tgenade@freeshell.org tel: +27-84-632-1925 (c) ******************************************************************************** "For there is one God, and there is one mediator between God and men, the man Christ Jesus, who gave himself as a ransom for all." 1 Timothy 2:5-6 From detmar <@t> mshri.on.ca Fri Jul 4 10:29:50 2008 From: detmar <@t> mshri.on.ca (Jacqui Detmar) Date: Fri Jul 4 10:30:06 2008 Subject: [Histonet] GS isolectin questions In-Reply-To: References: Message-ID: Hi Tyrone. I use that same lectin to stain mouse placental endothelial cells. I make a 1 mg/mL solution using PBS and then dilute it to 50 ug/mL (0.05 mg/mL), which is a 1:20 dilution. You could probably try a lower concentration, but I found for my mouse placentae that if I went lower, the stain became fainter. Good luck! Jacqui Jacqui Detmar, Post-doctoral Fellow Samuel Lunenfeld Research Institute, Mount Sinai Hospital 25 Orde Street, room 6-1001 AJ, Toronto, ON, Canada M5T 3H7 phone: 416-586-4800 x5607 fax: 416-586-8588 email: detmar@mshri.on.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tyrone Genade Sent: Friday, July 04, 2008 3:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GS isolectin questions Hello, I'm new to the list and the field of histology (busy startinga PhD). I have a question regarding GS BS-I Isolectin B4 (Sigma). This was the lectin used in the article by Kim et al (Neurobiology of Aging 25:491--500, 2004) for selective staining of CNS microglia. They state that it was used at a dilution of 1:50 which is amazingly helpful in light of the fact that no starting concentration is stated. I have been able to contact the corresponding author who gave me the email of the author who did the lectin work but no response... I have purchased 1 mg of the GS isolectin which has now arrived in powdered form. The recommendation by Sigma is to dissovle it in to 0.9% NaCl. Can anyone suggest a starting concentration from which I can make the 1:50 dilutions or better, suggest a good working solution concentration. I will be staining retinal sections of fish, not mammals, so anyone with advise pertinent to fish histology would be appreciated. I understand that as negative control I will have treat control sections with a lactose solution. Can anyone volunteer more information regarding control protocols? Also, I may want to counter stain with an antibody in frozen sections. Would the BSA blocking solution interfer with the lectin binding. We had previously been using tomato lectin with dismal results (it stuck to everything!). Many thanks Tyrone -- Tyrone Genade http://tgenade.freeshell.org email: tgenade@freeshell.org tel: +27-84-632-1925 (c) ************************************************************************ ******** "For there is one God, and there is one mediator between God and men, the man Christ Jesus, who gave himself as a ransom for all." 1 Timothy 2:5-6 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Anthony.Johnson <@t> leica-microsystems.com Fri Jul 4 16:01:07 2008 From: Anthony.Johnson <@t> leica-microsystems.com (Anthony.Johnson@leica-microsystems.com) Date: Fri Jul 4 16:01:18 2008 Subject: [Histonet] Johnson, Anthony is out of the office. Message-ID: I will be out of the office starting 07/04/2008 and will not return until 07/14/2008. I will be out of the country on vacation until Monday July 14th. If you wish customer service assistance please call Jamie Tinault on 847 405 7052 or email jamie.tinault@leica-microsystems.com. If you require Leica service then call 1 800 248 0123. I will respond to other enquiries on my return ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From marjoh3 <@t> telus.net Sat Jul 5 14:32:04 2008 From: marjoh3 <@t> telus.net (Marilyn Johnson) Date: Sat Jul 5 14:32:02 2008 Subject: [Histonet] Corynebacterium renale by Immunohistochemistry Staining Message-ID: <000801c8ded5$ce058e10$4101a8c0@VALUED20606295> Hi Histonetters, Our Lab is researching a new project to demonstrate Corynebacterium renale in bovine kidneys with pyelonephritis. Are there any IHC labs. out there that have used this technique? If so, please contact me and any replies to my request will be greatly appreciated. Thank you in advance. Happy summer holidays! Marilyn Johnson Alberta Agriculture Food Safety Division Edmonton, Alberta, Canada. From G.Spoelstra <@t> murdoch.edu.au Sun Jul 6 21:28:11 2008 From: G.Spoelstra <@t> murdoch.edu.au (Gerard Spoelstra) Date: Sun Jul 6 21:28:21 2008 Subject: [Histonet] "phosphotungstic-acid eosin method" Message-ID: Hi Could someone please give the details of this method. How successful is it in staining for viral inclusions? Thanks in advance. Gerard Spoelstra Murdoch University From akbitting <@t> geisinger.edu Mon Jul 7 06:59:28 2008 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Mon Jul 7 06:59:35 2008 Subject: [Histonet] H&E quality check Message-ID: <4871CCE0.2B7F.00C9.0@geisinger.edu> Just curious as to how other hospital labs quality check their H&E slides? Do most review a percentage microscopically? IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From b-frederick <@t> northwestern.edu Mon Jul 7 07:36:31 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Jul 7 07:36:45 2008 Subject: [Histonet] (no subject) In-Reply-To: Message-ID: <002001c8e02e$1900f630$d00f7ca5@lurie.northwestern.edu> All, I have a researcher that likes me to do the trichrome over the EVG. It's a combo of both stains. Looks gorgeous. I use an aorta as the control when I do it.She also has me do Goldner's trichrome on occasion. Looks good on heart muscle. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Thursday, July 03, 2008 6:02 PM To: Sheree Beam; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (no subject) Sheree, Masson's stain is quite capricious. Unlike the van Gieson stains, over differentiation of the red muscle stain is common. The use of mordants can often help. Try placing hydrated slides for 30min in Bouins solution (or aqueous saturated picric acid) at 60oC prior to staining. With the use of the blue collagen counterstain, confusion can also occur since under-differentiation of the nuclear stain, Celestine blue-Harris's Hx or some variant of the iron haematoxylin, can render the muscle blue. The blue you are describing could be due to overstaining with the celestine blue-Hx rather than the iron Hx which would look black. Again overstaining with the blue dye of the Massons as well as overdifferentiation of the red dye would render muscle blue. In my experience control sections rarely behave like diagnostic slides with the Massons. Different fixation times and older control sections change in their affinity for the red and blue stins of the Massons. Try a green collagen stain rather than the blue. The photos may look more pleasing or have you considered using a Van Gieson's stain in stead? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheree Beam Sent: Friday, 4 July 2008 5:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Greetings, I received Masson's trichrome stains of arteries and adjacent muscle from a histolab. The first time I received them, the entire artery was blue and the adjacent muscle was purple to sometimes red. The smooth muscle in the arteries were completely blue. However the control tissue of kidney which included a small artery stained perfectly. I asked for the tissue to be recut and try again with Masson's. This time the smooth muscle in the artery has some magenta, but in other areas the blue prevailed. More controls were run and all were perfect. The tissues were fixed in 10% neutral buffered formalin for 10 or 30 days as there were 2 groups. We used two different boxes of formalin. After trim, the tissues were placed back into formalin and sent to the histo lab. There was no fixation in ethanol. Are there any explanations out there? Is there anything we can do? I need the trichromes for photomicrographs. Would a different trichrome stain potentially help? All input would be appreciated. Thank you, Sheree Sheree Beam, DVM, MS, ACVP, Diplomate Veterinary Pathologist Preclinical Pathology Consulting Service, LLC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From stephanie.d.rivera <@t> gsk.com Mon Jul 7 08:49:09 2008 From: stephanie.d.rivera <@t> gsk.com (stephanie.d.rivera@gsk.com) Date: Mon Jul 7 08:49:29 2008 Subject: [Histonet] H&E quality check In-Reply-To: <4871CCE0.2B7F.00C9.0@geisinger.edu> Message-ID: Everywhere I have worked there was a liver control slide run daily for Q.C. The techs checked the staining quality before beginning staining for the day. The slide was dated and documented in the log book. Stephanie D. Rivera Safety Assessment Department GlaxoSmithKline 709 Swedeland RD King of Prussia, PA 19406 phone: 610-270-7340 fax: 610-270-7202 "Angela Bitting" Sent by: histonet-bounces@lists.utsouthwestern.edu 07-Jul-2008 07:59 To "histonet" cc Subject [Histonet] H&E quality check Just curious as to how other hospital labs quality check their H&E slides? Do most review a percentage microscopically? IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Jul 7 08:58:29 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jul 7 08:58:32 2008 Subject: [Histonet] H&E quality check In-Reply-To: <4871CCE0.2B7F.00C9.0@geisinger.edu> Message-ID: <110123.40666.qm@web65709.mail.ac4.yahoo.com> Because of all the tissues/cells included, we always ran an appendix section with each batch of slides. The quality was checked, the results added?to a log, and the slides kept with the date. Ren? J. --- On Mon, 7/7/08, Angela Bitting wrote: From: Angela Bitting Subject: [Histonet] H&E quality check To: "histonet" Date: Monday, July 7, 2008, 7:59 AM Just curious as to how other hospital labs quality check their H&E slides? Do most review a percentage microscopically? IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histology <@t> gradymem.org Mon Jul 7 10:49:54 2008 From: histology <@t> gradymem.org (Histology Dept) Date: Mon Jul 7 10:50:00 2008 Subject: [Histonet] Gomeri Iron Stain Message-ID: We finally got a microwave for special stains. Can you use a microwave method to do Gomeri Iron Stain? If so, would someone please share you procedure with us? Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org From SmithSR <@t> crstlukes.com Mon Jul 7 11:02:46 2008 From: SmithSR <@t> crstlukes.com (Smith, Susan R.) Date: Mon Jul 7 11:03:23 2008 Subject: [Histonet] Job opportunity in the Midwest Message-ID: We are looking for full and part time histotechs at St. Luke's Hospital in Cedar Rapids, Iowa. If interested, please contact the Human Resources Department at St. Luke's Hospital, Cedar Rapids, Iowa at 319-369-7275. You may also apply on-line at our website www.stlukescr.org. Come see how we survived the flood of 2008. Sue Smith, Laboratory Supervisor St. Luke's Hospital 1026 A Ave NE Cedar Rapids, IA 52402 ph: 319-369-8845 fax: 319-369-8095 e-mail: smithsr@crstlukes.com ******************************************** This message and accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. ?? 2510-2521, and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ********************************************* From ht.ascp <@t> yahoo.com Mon Jul 7 11:04:06 2008 From: ht.ascp <@t> yahoo.com (Jon Google) Date: Mon Jul 7 11:04:11 2008 Subject: [Histonet] H&E quality check In-Reply-To: <4871CCE0.2B7F.00C9.0@geisinger.edu> Message-ID: <922077.65259.qm@web59914.mail.ac4.yahoo.com> We run one control prior to start the day's work. Then we check one slide per rack and document that in a log book.? This will also help look at quality in sections prior to turning in cases. --- On Mon, 7/7/08, Angela Bitting wrote: From: Angela Bitting Subject: [Histonet] H&E quality check To: "histonet" Date: Monday, July 7, 2008, 11:59 AM Just curious as to how other hospital labs quality check their H&E slides? Do most review a percentage microscopically? IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kenneth.metzger <@t> aruplab.com Mon Jul 7 12:33:15 2008 From: kenneth.metzger <@t> aruplab.com (Metzger, Kenneth) Date: Mon Jul 7 12:31:53 2008 Subject: [Histonet] Metal Slide Trays Message-ID: Does anyone know where I can order the 20 slot metal slide trays? Thanks, ken Ken Metzger HTL(ASCP) Histology Supervisor ARUP Laboratories 500 Chipeta way Salt Lake City, UT 84108 801.583.2787 ext 3101 - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From CBark <@t> memorialcare.org Mon Jul 7 12:37:27 2008 From: CBark <@t> memorialcare.org (Christine Bark) Date: Mon Jul 7 12:36:57 2008 Subject: [Histonet] RE: H&E quality check In-Reply-To: <646C93BA1TG997896-01@emf1.memorialcare.org> Message-ID: We check several different slides from our first rack of the day for H&E and cutting quality. Christine Bark HT(ASCP) Senior Histotech, Pathology Saddleback Memorial Medical Center 949-452-3548 cbark@memorialcare.org -----Original Message----- From: "Angela Bitting" Subject: [Histonet] H&E quality check Just curious as to how other hospital labs quality check their H&E slides? Do most review a percentage microscopically? ______________________________________________________________________________ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From jqb7 <@t> cdc.gov Mon Jul 7 12:40:13 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Mon Jul 7 12:40:26 2008 Subject: [Histonet] Metal Slide Trays In-Reply-To: References: Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A7F23C7E@LTA3VS011.ees.hhs.gov> I think this is what you are looking for. http://www.daigger.com/catalog/product?deptId=&prodId=16050 Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Metzger, Kenneth Sent: Monday, July 07, 2008 1:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Metal Slide Trays Does anyone know where I can order the 20 slot metal slide trays? Thanks, ken Ken Metzger HTL(ASCP) Histology Supervisor ARUP Laboratories 500 Chipeta way Salt Lake City, UT 84108 801.583.2787 ext 3101 - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rosenfeldtek <@t> hotmail.com Mon Jul 7 12:54:49 2008 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Mon Jul 7 12:54:54 2008 Subject: [Histonet] Vector Labs Nova HRP--Red Substrate: Not Really EtOH compatible? In-Reply-To: References: <646C93BA1TG997896-01@emf1.memorialcare.org> Message-ID: The Product information says: "NovaRED? substrate may be dehydrated in ethanol and cleared in organic solvents such as xylene and permanently mounted in a non-aqueous mounting medium such as VectaMount" But I have done two stains now where I initially had intense, specific staining at first--But after my slides come out of the EtOH/Xylene for coverslipping, the staining has vanished. This is getting to be expensive! Has anyone else ever had this problem with Nova Red? Any ideas? Jerry Ricks Research Scientist University of Washington Department of Pathology _________________________________________________________________ Need to know now? Get instant answers with Windows Live Messenger. http://www.windowslive.com/messenger/connect_your_way.html?ocid=TXT_TAGLM_WL_messenger_072008 From POWELL_SA <@t> Mercer.edu Mon Jul 7 13:07:27 2008 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Mon Jul 7 13:12:47 2008 Subject: [Histonet] Help needed for brain processing Message-ID: <01MWVKZ3Y6Y200ANAP@Macon2.Mercer.edu> A friend of mine, who has been banned from histonet by a micro-managing employer, needs advice from those who use the microwave to expedite fixation of whole or partial brains for routine processing, or any other rapid means of getting complete fixation on brain. Please send all responses to me personally at this address and I will forward the information to them. Thanks in advance for your help. Shirley Powell From alexandra.meinl <@t> gmail.com Mon Jul 7 13:24:22 2008 From: alexandra.meinl <@t> gmail.com (Alexandra Meinl) Date: Mon Jul 7 13:24:26 2008 Subject: [Histonet] Antibody for the detection of human cells needed Message-ID: Hi all, I'm searching for an antibody for the detection of human cells in mouse tissue. And: we don't know what type of cells we're trying to find. I thought about an ab like a-hu-nuclear membrane or GM 130. Does anybody this? What antibody would you recommend? Any suggestions greatly appreaciated! Alexandra From gentras <@t> vetmed.auburn.edu Mon Jul 7 13:35:13 2008 From: gentras <@t> vetmed.auburn.edu (Atoska Gentry) Date: Mon Jul 7 13:35:18 2008 Subject: [Histonet] tissue processor Message-ID: <487261E1.3020101@vetmed.auburn.edu> hello, please will someone who has info on a modern tissue processor comparable to an obsolete 25 yr.Autotechnicon Ultra II Tissue Processor please contact me with specifications, product info & pricing ASAP? Thanks, Atoska From Maxim_71 <@t> mail.ru Mon Jul 7 13:30:20 2008 From: Maxim_71 <@t> mail.ru (Maxim_71@mail.ru) Date: Mon Jul 7 13:35:55 2008 Subject: [Histonet] Gomeri Iron Stain Message-ID: <228377341.20080707223020@mail.ru> Angie: We never used MWO modification for Gomori Iron Stain. Iron is beautifully can demonsrtate at RT for 10 mins. Vapours of cyanid is very toxic for personnel. Maxim Peshkov Russia, Taganrog. From sharon.osborn <@t> comcast.net Mon Jul 7 14:11:41 2008 From: sharon.osborn <@t> comcast.net (sharon.osborn@comcast.net) Date: Mon Jul 7 14:11:49 2008 Subject: [Histonet] Leitz 1512 rotary microtome Message-ID: <070720081911.18025.48726A6D0006F323000046692215555884029D010D9C01D202019D0E089C@comcast.net> Histonetters, I have a copy of the instructions manual for above referenced microtome should anyone still have this workhorse and need the instructions. Please send me your contact information and I am happy to send out. If there is more than one person requesting it, I can make copies. sharon osborn Lab Vision Thermo Fisher Fremont, CA From liz <@t> premierlab.com Mon Jul 7 14:20:33 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Mon Jul 7 14:20:43 2008 Subject: [Histonet] Antibody for the detection of human cells needed In-Reply-To: References: Message-ID: Alexandra We have tried the human anti-nuclear antibody from chemicon but we did not have much success with it. We have used a human mitochondrial antibody from Novus with good success but in rat tissues rather than mouse, it's generated in mouse but as long as you use the correct controls it should work. We have also ran In-situ with a repeat sequence for human DNA and that worked nicely between mouse and also rat and human cells. We had our probe made by GeneDetect. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alexandra Meinl Sent: Monday, July 07, 2008 12:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antibody for the detection of human cells needed Hi all, I'm searching for an antibody for the detection of human cells in mouse tissue. And: we don't know what type of cells we're trying to find. I thought about an ab like a-hu-nuclear membrane or GM 130. Does anybody this? What antibody would you recommend? Any suggestions greatly appreaciated! Alexandra _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Mon Jul 7 14:34:27 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Mon Jul 7 14:34:37 2008 Subject: [Histonet] Re: H&E quality check Message-ID: Well, I've worked in roughly 60 hospital and private lab pathology services in my locum tenens career, and I've never seen a functioning QA (or whatever they call it this year) program for H & E (or any other stain) in a pathology lab. An occasional lab has the pathologist fill out QA sheets telling them that the slides are wonderful - invariably in such labs the slides are horrible. In most labs nobody but the pathologist ever looks at a slide under a microscope. A meaningful QA program would have a pathologist and a senior histotechnologist review some of the day's production, during the working day. I've always been ridiculed for suggesting it. Bob Richmond Samurai Pathologist Knoxville TN From akbitting <@t> geisinger.edu Mon Jul 7 14:47:21 2008 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Mon Jul 7 14:47:36 2008 Subject: [Histonet] H&E quality check In-Reply-To: References: <4871CCE0.2B7F.00C9.0@geisinger.edu> Message-ID: <48723A89.2B7F.00C9.0@geisinger.edu> Thanks for all of the replies. I guess I need to rephrase my question. I'm wondering how many slides are reviewed daily under the scope for cutting artifacts, incomplete margins, etc. Where I worked before, we checked 10% of the slides under the scope before sending them on to the Pathologists. Do other labs do this? >>> 7/7/2008 9:49 AM >>> Everywhere I have worked there was a liver control slide run daily for Q.C. The techs checked the staining quality before beginning staining for the day. The slide was dated and documented in the log book. Stephanie D. Rivera Safety Assessment Department GlaxoSmithKline 709 Swedeland RD King of Prussia, PA 19406 phone: 610-270-7340 fax: 610-270-7202 "Angela Bitting" Sent by: histonet-bounces@lists.utsouthwestern.edu 07-Jul-2008 07:59 To "histonet" cc Subject [Histonet] H&E quality check Just curious as to how other hospital labs quality check their H&E slides? Do most review a percentage microscopically? IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> pathview.com Mon Jul 7 14:50:45 2008 From: mike <@t> pathview.com (Michael Mihalik) Date: Mon Jul 7 14:51:11 2008 Subject: [Histonet] Re: H&E quality check In-Reply-To: References: Message-ID: To tag along with this statement. Wouldn't it be nice if the pathologist could comment on the 'quality' of the stain as they are reviewing the slide in the first place? ...or am I missing something here? Michael Mihalik PathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Monday, July 07, 2008 3:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: H&E quality check Well, I've worked in roughly 60 hospital and private lab pathology services in my locum tenens career, and I've never seen a functioning QA (or whatever they call it this year) program for H & E (or any other stain) in a pathology lab. An occasional lab has the pathologist fill out QA sheets telling them that the slides are wonderful - invariably in such labs the slides are horrible. In most labs nobody but the pathologist ever looks at a slide under a microscope. A meaningful QA program would have a pathologist and a senior histotechnologist review some of the day's production, during the working day. I've always been ridiculed for suggesting it. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LINDA.MARGRAF <@t> childrens.com Mon Jul 7 15:02:20 2008 From: LINDA.MARGRAF <@t> childrens.com (LINDA MARGRAF) Date: Mon Jul 7 15:03:11 2008 Subject: [Histonet] HT position available Message-ID: <48722FFC020000DA0002B8BF@CNET3.CHILDRENS.COM> Here's a message Beth asked me to post for her. Please reply to her if you are interested. Thanks LM (histonet administrator) Technical Applications specialist- laboratory ESSENTIAL DUTIES AND RESPONSIBILITIES Installation of instrumentation and conducting user training to successfully integrate products within the customer laboratory. Provide troubleshooting and minor repair to instrumentation. Work with customers to resolve reagent/staining application issues. Communicate with dispatch and area management on daily/weekly/monthly activities (eg. Salesforce.com) Maintaining tools and test equipment in accordance with Quality procedures Conduct in-service training for customers. Customer follow-up Submit administrative paperwork in a timely and accurate manner. installation forms service work orders expense reports Travel extensively within the region and throughout the country. REQUIRED EDUCATION & EXPERIENCE Associates degree (or equivalent) or higher in Applied Sciences or accredited Medical/Histology Technology course. Minimum one year general clinical laboratory experience or technical equivalent which may include general histology, cytology, immunohistochemistry, in situ hybridization, biology research or work with automated staining instrumentation. CERTIFICATES, LICENSES, REGISTRATIONS Highly desirable: HISTOLOGY TECHNICIAN American Society of Clinical Pathologists Certification HT or HTL. American Society of Clinical Pathologists Qualification in Immunohistochemistry (QIHC). KNOWLEDGE, SKILLS & ABILITIES Must have the ability to read, analyze, and interpret technical procedures or governmental regulations (CAP, CLIA). Ability to write reports. Ability to effectively present information and respond to questions from customers. Ability to calculate antibody dilutions. Ability to interpret a variety of instructions furnished in written, verbal, diagram, or schedule form. WORK ENVIRONMENT The work environment characteristics described here are representative of those an employee encounters while performing the essential functions of this job traveling to and working within a Clinical Pathology Lab, Research lab, and animal diagnostic lab. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions. OTHER QUALIFICATIONS Ability to travel. Valid driver*s license. with a clean driving record. COMPENSATION PACKAGE POSITION OFFERS A COMPETITVE BASE SALARY AND A CAR PLUS ALL TRAVEL EXPENSES AND BENEFITS. CONTACT BETH TEWS RECRUITING BETHTEWS@HOTMAIL.COM 623-742-7227 OPENINGS CURRENTLY NEED TO BE FILLED BY 715/08 IN PHOENIX, NYC/NJ,ST LOUIS, BOSTON MORE TO COME SO PLEASE APPLY Please consider the environment before printing this e-mail

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From JWeems <@t> sjha.org Mon Jul 7 15:14:06 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Mon Jul 7 15:14:12 2008 Subject: [Histonet] H&E quality check In-Reply-To: <48723A89.2B7F.00C9.0@geisinger.edu> References: <4871CCE0.2B7F.00C9.0@geisinger.edu> <48723A89.2B7F.00C9.0@geisinger.edu> Message-ID: <982A0A9461F9BF438C7B19A6E425A383373163@ITSSSXM01V6.one.ads.che.org> We do 1 or 2 per full tray - recording wrinkles, folds, poor staining, etc. to get an approximate 10% reviewed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Monday, July 07, 2008 3:47 PM To: stephanie.d.rivera@gsk.com Cc: histonet; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] H&E quality check Thanks for all of the replies. I guess I need to rephrase my question. I'm wondering how many slides are reviewed daily under the scope for cutting artifacts, incomplete margins, etc. Where I worked before, we checked 10% of the slides under the scope before sending them on to the Pathologists. Do other labs do this? >>> 7/7/2008 9:49 AM >>> Everywhere I have worked there was a liver control slide run daily for Q.C. The techs checked the staining quality before beginning staining for the day. The slide was dated and documented in the log book. Stephanie D. Rivera Safety Assessment Department GlaxoSmithKline 709 Swedeland RD King of Prussia, PA 19406 phone: 610-270-7340 fax: 610-270-7202 "Angela Bitting" Sent by: histonet-bounces@lists.utsouthwestern.edu 07-Jul-2008 07:59 To "histonet" cc Subject [Histonet] H&E quality check Just curious as to how other hospital labs quality check their H&E slides? Do most review a percentage microscopically? IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From Charlene.Henry <@t> STJUDE.ORG Mon Jul 7 15:14:08 2008 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Mon Jul 7 15:14:15 2008 Subject: [Histonet] Re: H&E quality check. . In-Reply-To: Message-ID: <03E1F5968F60C5448635D49D38B283ED01460FD918@SJMEMXMBS11.stjude.sjcrh.local> Michael, Actually it is now a CAP requirement. ANP.11713 Is there documented evidence of daily review of the technical quality of histologic preparations by the pathologist. This is the way we address this question. Each morning while we are cutting surgical and bone marrow biopsies, we randomly select 2-3 blocks and cut a QC H&E slide in addition to the routine cuts. They are stained along the routine cases and these are given to our Director of AP for review. She then documents the quality of fixation, processing, cutting and staining on a log. While she is on vacation one of our other pathologists review the slides. When the log sheet is full, it is returned to me and filed with our QC records. Charlene Henry HT (ASCP), QIHC Anatomic Pathology Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Monday, July 07, 2008 2:51 PM To: 'Robert Richmond'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: H&E quality check. . To tag along with this statement. Wouldn't it be nice if the pathologist could comment on the 'quality' of the stain as they are reviewing the slide in the first place? ...or am I missing something here? Michael Mihalik PathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Monday, July 07, 2008 3:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: H&E quality check Well, I've worked in roughly 60 hospital and private lab pathology services in my locum tenens career, and I've never seen a functioning QA (or whatever they call it this year) program for H & E (or any other stain) in a pathology lab. An occasional lab has the pathologist fill out QA sheets telling them that the slides are wonderful - invariably in such labs the slides are horrible. In most labs nobody but the pathologist ever looks at a slide under a microscope. A meaningful QA program would have a pathologist and a senior histotechnologist review some of the day's production, during the working day. I've always been ridiculed for suggesting it. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> pathview.com Mon Jul 7 15:47:31 2008 From: mike <@t> pathview.com (Michael Mihalik) Date: Mon Jul 7 15:48:00 2008 Subject: [Histonet] Re: H&E quality check. . In-Reply-To: <03E1F5968F60C5448635D49D38B283ED01460FD918@SJMEMXMBS11.stjude.sjcrh.local> References: <03E1F5968F60C5448635D49D38B283ED01460FD918@SJMEMXMBS11.stjude.sjcrh.local> Message-ID: I appreciate the response. Let me ask this question another way. Is there anybody who currently logs their histo QC in their computer system, not on paper, not on some department created excel spreadsheet? Would this not be desirable? Michael Mihalik PathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: Henry, Charlene [mailto:Charlene.Henry@STJUDE.ORG] Sent: Monday, July 07, 2008 4:14 PM To: 'Michael Mihalik'; 'Robert Richmond'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: H&E quality check. . Michael, Actually it is now a CAP requirement. ANP.11713 Is there documented evidence of daily review of the technical quality of histologic preparations by the pathologist. This is the way we address this question. Each morning while we are cutting surgical and bone marrow biopsies, we randomly select 2-3 blocks and cut a QC H&E slide in addition to the routine cuts. They are stained along the routine cases and these are given to our Director of AP for review. She then documents the quality of fixation, processing, cutting and staining on a log. While she is on vacation one of our other pathologists review the slides. When the log sheet is full, it is returned to me and filed with our QC records. Charlene Henry HT (ASCP), QIHC Anatomic Pathology Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Monday, July 07, 2008 2:51 PM To: 'Robert Richmond'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: H&E quality check. . To tag along with this statement. Wouldn't it be nice if the pathologist could comment on the 'quality' of the stain as they are reviewing the slide in the first place? ...or am I missing something here? Michael Mihalik PathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Monday, July 07, 2008 3:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: H&E quality check Well, I've worked in roughly 60 hospital and private lab pathology services in my locum tenens career, and I've never seen a functioning QA (or whatever they call it this year) program for H & E (or any other stain) in a pathology lab. An occasional lab has the pathologist fill out QA sheets telling them that the slides are wonderful - invariably in such labs the slides are horrible. In most labs nobody but the pathologist ever looks at a slide under a microscope. A meaningful QA program would have a pathologist and a senior histotechnologist review some of the day's production, during the working day. I've always been ridiculed for suggesting it. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Mon Jul 7 18:19:30 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Jul 7 18:19:41 2008 Subject: [Histonet] Gomeri Iron Stain In-Reply-To: Message-ID: The following works well though it is a Perl's stain. I can't remember the Gomori Iron stain. The old cranium is shrinking as I get older!! It was developed for frozen sections (eg differentiating melanin from iron pigment) but works quite well for paraffin sections. 1 Placed ethanol fixed frozen sections or hydrated paraffin section in an the Perl's reagent in a plastic coplin jar (20ml each of 2% Potassium ferrocyanide and 2% HCl). Microwave for 20 sec at 650watts. 2. Leave sections in the hot solution for up to 3 minutes, remove if the solution becomes cloudy. 3. Rinse in water. 4. Counterstain with eosin (as used for the frozen section H&E) or a red nuclear stain (Nuclear Fast Red or Neutral red). 5. Rinse in water, DC&M. Reference: Henwood (2002) "Microwave Perl's Stain for Urgent Frozen Sections" Aust J Med Sc 23(2):68-69. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Histology Dept Sent: Tuesday, 8 July 2008 1:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gomeri Iron Stain We finally got a microwave for special stains. Can you use a microwave method to do Gomeri Iron Stain? If so, would someone please share you procedure with us? Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From lmayhew5 <@t> cogeco.ca Mon Jul 7 19:45:29 2008 From: lmayhew5 <@t> cogeco.ca (Dave & Lee Mayhew) Date: Mon Jul 7 19:45:23 2008 Subject: [Histonet] Re: H&E quality check. Message-ID: <008601c8e093$eb805690$e013c143@user4ab93baede> Hi Michael, We use Meditech and create a daily QC specimen. In the data fields for the QC specimen we record all the stains we did for that day, including H&E, and all the specimen numbers that were controlled by those QC slides. Any problems, concerns, repeats, etc. are recorded in the specimen comment field. A daily report is printed out at the end of the day and filed in the QC binder. Lee Mayhew MLT St. Joseph's Hospital Hamilton ON Canada From mike <@t> pathview.com Mon Jul 7 20:16:13 2008 From: mike <@t> pathview.com (Michael Mihalik) Date: Mon Jul 7 20:16:43 2008 Subject: [Histonet] Re: H&E quality check. In-Reply-To: <008601c8e093$eb805690$e013c143@user4ab93baede> References: <008601c8e093$eb805690$e013c143@user4ab93baede> Message-ID: <772604D2DC974271BCC24D75ED3FA5ED@MDMM1330> So you create a 'normal' case where the specimen is a qc case and then fill in all the 'normal' fields with qc data. Is that correct? ....or are there specialized qc data fields, like a prompt for stain quality, block quality, etc. I'm looking to see if any computer system does anything special for QC purposes, relative to block, slides, stains, etc. Michael Mihalik PathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dave & Lee Mayhew Sent: Monday, July 07, 2008 8:45 PM To: Histonet Subject: [Histonet] Re: H&E quality check. Hi Michael, We use Meditech and create a daily QC specimen. In the data fields for the QC specimen we record all the stains we did for that day, including H&E, and all the specimen numbers that were controlled by those QC slides. Any problems, concerns, repeats, etc. are recorded in the specimen comment field. A daily report is printed out at the end of the day and filed in the QC binder. Lee Mayhew MLT St. Joseph's Hospital Hamilton ON Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lmayhew5 <@t> cogeco.ca Mon Jul 7 20:34:08 2008 From: lmayhew5 <@t> cogeco.ca (Dave & Lee Mayhew) Date: Mon Jul 7 20:34:04 2008 Subject: [Histonet] Re: H&E quality check. References: <008601c8e093$eb805690$e013c143@user4ab93baede> <772604D2DC974271BCC24D75ED3FA5ED@MDMM1330> Message-ID: <00a501c8e09a$b7876bb0$e013c143@user4ab93baede> Michael The case created is a special QC Specimen. Meditech allows you to create any data fields that you want, they do not have to be the same data sections as are found in patient specimens, so you could create a prompt for the things you suggest if you wanted to. The workload that you enter into this specimen is counted as quality control not patient workload. I should say that we also have a daily sheet that the day's pathologist fills out to document any of their concerns. We do not enter this into Meditech, although we, or they, could. Lee Mayhew MLT > St. Joseph's Hospital > Hamilton ON Canada From annigyg <@t> gmail.com Mon Jul 7 23:52:10 2008 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Mon Jul 7 23:52:14 2008 Subject: [Histonet] Re: H&E quality check. . In-Reply-To: References: <03E1F5968F60C5448635D49D38B283ED01460FD918@SJMEMXMBS11.stjude.sjcrh.local> Message-ID: we have a tissue array (tonsil, kidney, skin, L/N, appendix) block which we made ourselves by using a dermatology punch tool identical slides are cut and kept in a box each morning we stain one and it gets viewed by a senior tech - the path does not have time for such matters!! results logged, adjustments noted, signed off, logs filed, slides filed - all ready for CAP to scrutinise if we adjust the stain (or anything else) we run another QC slide and relog the results we are in the process of making an extended array for a whole bunch of specials too its a pain in the @$$ but my staff are used to the process now ideally it should be done the 'samurai' way - but im just the tech - what do i know!!! ;)) annieinarabia 2008/7/8 Michael Mihalik : > I appreciate the response. Let me ask this question another way. Is there > anybody who currently logs their histo QC in their computer system, not on > paper, not on some department created excel spreadsheet? > > Would this not be desirable? > > > Michael Mihalik > PathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540 > | fax: 270.423.0968 > > > > > -----Original Message----- > From: Henry, Charlene [mailto:Charlene.Henry@STJUDE.ORG] > Sent: Monday, July 07, 2008 4:14 PM > To: 'Michael Mihalik'; 'Robert Richmond'; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: H&E quality check. . > > Michael, > Actually it is now a CAP requirement. > > ANP.11713 Is there documented evidence of daily review of the technical > quality of histologic preparations by the pathologist. > > This is the way we address this question. > Each morning while we are cutting surgical and bone marrow biopsies, we > randomly select 2-3 blocks and cut a QC H&E slide in addition to the > routine > cuts. They are stained along the routine cases and these are given to our > Director of AP for review. She then documents the quality of fixation, > processing, cutting and staining on a log. While she is on vacation one of > our other pathologists review the slides. When the log sheet is full, it is > returned to me and filed with our QC records. > > > Charlene Henry HT (ASCP), QIHC > Anatomic Pathology Section Head > Department of Pathology > St. Jude Children's Research Hospital > 901-495-3191 > fax 901-495-3100 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael > Mihalik > Sent: Monday, July 07, 2008 2:51 PM > To: 'Robert Richmond'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: H&E quality check. . > > To tag along with this statement. Wouldn't it be nice if the pathologist > could comment on the 'quality' of the stain as they are reviewing the slide > in the first place? > > ...or am I missing something here? > > Michael Mihalik > PathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540 > | fax: 270.423.0968 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert > Richmond > Sent: Monday, July 07, 2008 3:34 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: H&E quality check > > Well, I've worked in roughly 60 hospital and private lab pathology services > in my locum tenens career, and I've never seen a functioning QA (or > whatever > they call it this year) program for H & E (or any other stain) in a > pathology lab. An occasional lab has the pathologist fill out QA sheets > telling them that the slides are wonderful - invariably in such labs the > slides are horrible. In most labs nobody but the pathologist ever looks at > a > slide under a microscope. > > A meaningful QA program would have a pathologist and a senior > histotechnologist review some of the day's production, during the working > day. I've always been ridiculed for suggesting it. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From rjbuesa <@t> yahoo.com Tue Jul 8 06:46:40 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jul 8 06:46:45 2008 Subject: [Histonet] H&E quality check In-Reply-To: <48723A89.2B7F.00C9.0@geisinger.edu> Message-ID: <661700.14357.qm@web65702.mail.ac4.yahoo.com> If you get to a percentage, then you will waste your whole day doing that. What I used to do is to let the pathologists reject the slides, then I reviewed them, found out who cut them, discuss the issue, fill the QA and retrain the HT who did the bad slide.Any other approach will be a total loss of precious time.Don't even think if giving a form to the pathologists to fill out the problem they found, they are not going to do it, and if they do, they will be wasting their time. That is the supervisor's task, the PT task is to reject, the supervisor's task is to prevent the problem from occurring again. Ren? J. --- On Mon, 7/7/08, Angela Bitting wrote: ? From cmiller <@t> physlab.com Tue Jul 8 07:05:20 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Tue Jul 8 07:06:24 2008 Subject: [Histonet] Re: H&E quality check. . In-Reply-To: References: <03E1F5968F60C5448635D49D38B283ED01460FD918@SJMEMXMBS11.stjude.sjcrh.local> Message-ID: <000901c8e0f2$e4e24280$3d02a8c0@plab.local> We check the slide quality daily by using a control slide (usually an appendix or gallbladder) with the first rack of slides. I then check random slides from each rack stained, we also have a QA sheet the paths fill out daily. They grade us on grossing, specimen sampling, processing, embedding, microtomy, staining and cover slipping. Most of them see this as a true quality control indicator and let us know when we have an issue; a few just fill it out without any real critiquing, which defeats the whole purpose. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From annigyg <@t> gmail.com Tue Jul 8 07:16:09 2008 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Tue Jul 8 07:16:20 2008 Subject: [Histonet] H&E quality chec In-Reply-To: <661700.14357.qm@web65702.mail.ac4.yahoo.com> References: <48723A89.2B7F.00C9.0@geisinger.edu> <661700.14357.qm@web65702.mail.ac4.yahoo.com> Message-ID: ok - but CAP want proof of daily H&E QC signed off by the lab director or designee PTs dont really care about the loss of our time - mine appear to believe that it is their sole purpose in life to waste our time with their sometimes 'amazing' requests - had one the other day asking for a bielshowsky on a heart valve!!! he is a bit dyslexic and got the case number mixed up - luckily we did the usual 'diaper change' and corrected his error i was told the other day that we (med techs) are here to 'serve the PTS' - to 'do their bidding' ..... ;)) annieinarabia 2008/7/8 Rene J Buesa : > If you get to a percentage, then you will waste your whole day doing that. > What I used to do is to let the pathologists reject the slides, then I > reviewed them, found out who cut them, discuss the issue, fill the QA and > retrain the HT who did the bad slide.Any other approach will be a total loss > of precious time.Don't even think if giving a form to the pathologists to > fill out the problem they found, they are not going to do it, and if they > do, they will be wasting their time. That is the supervisor's task, the PT > task is to reject, the supervisor's task is to prevent the problem from > occurring again. > Ren? J. > > --- On Mon, 7/7/08, Angela Bitting wrote: > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From MDiCarlo <@t> KaleidaHealth.Org Tue Jul 8 07:38:58 2008 From: MDiCarlo <@t> KaleidaHealth.Org (DiCarlo, Margaret) Date: Tue Jul 8 07:39:06 2008 Subject: [Histonet] gutterguard Message-ID: <1B73766A27A1554CB2729B6432E81301B2F70D@KALEXMB04.KaleidaHealth.org> Hello Histonetters, Does anyone know where I can purchase rolled metal gutterguard that I use for decaling and processing bones? I used to be able to buy it at Mr. Seconds which is now called the Bargain Outlet but they no longer carry this product. I have tried Home Depot and Valu but they only have the rolled plastic gutterguard which I think the decal or xylene will dissolve. I appreciate your suggestions in advance. Peggy DiCarlo HT (ASCP) Orthopedic Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 2007 Best Places to Work Finalist Visit our careers page at www.kaleidahealth.org/careers CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. 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From annigyg <@t> gmail.com Tue Jul 8 07:48:14 2008 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Tue Jul 8 07:48:18 2008 Subject: [Histonet] H&E quality chec In-Reply-To: <661700.14357.qm@web65702.mail.ac4.yahoo.com> References: <48723A89.2B7F.00C9.0@geisinger.edu> <661700.14357.qm@web65702.mail.ac4.yahoo.com> Message-ID: ok - but CAP want proof of daily H&E QC signed off by the lab director or designee PTs dont really care about the loss of our time - mine appear to believe that it is their sole purpose in life to waste our time with their sometimes 'amazing' requests - had one the other day asking for a bielshowsky on a heart valve!!! he is a bit dyslexic and got the case number mixed up - luckily we did the usual 'diaper change' and corrected his error i was told the other day that we (med techs) are here to 'serve the PTS' - to 'do their bidding' ..... ;)) annieinarabia 2008/7/8 Rene J Buesa : > If you get to a percentage, then you will waste your whole day doing that. > What I used to do is to let the pathologists reject the slides, then I > reviewed them, found out who cut them, discuss the issue, fill the QA and > retrain the HT who did the bad slide.Any other approach will be a total loss > of precious time.Don't even think if giving a form to the pathologists to > fill out the problem they found, they are not going to do it, and if they > do, they will be wasting their time. That is the supervisor's task, the PT > task is to reject, the supervisor's task is to prevent the problem from > occurring again. > Ren? J. > > --- On Mon, 7/7/08, Angela Bitting wrote: > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From rshooki_99 <@t> yahoo.com Tue Jul 8 07:55:22 2008 From: rshooki_99 <@t> yahoo.com (richard shook) Date: Tue Jul 8 07:55:34 2008 Subject: [Histonet] Re: Histonet Digest, Vol 56, Issue 9 Message-ID: <527965.16532.qm@web36103.mail.mud.yahoo.com> VWR has the metal slide trays? #48469-004 ----- Original Message ---- From: "histonet-request@lists.utsouthwestern.edu" To: histonet@lists.utsouthwestern.edu Sent: Tuesday, July 8, 2008 8:06:49 AM Subject: Histonet Digest, Vol 56, Issue 9 Send Histonet mailing list submissions to ??? histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ? 1. Metal Slide Trays (Metzger, Kenneth) ? 2. RE: H&E quality check (Christine Bark) ? 3. RE: Metal Slide Trays (Bartlett, Jeanine (CDC/CCID/NCZVED)) ? 4. Vector Labs Nova HRP--Red Substrate: Not Really EtOH ? ? ? compatible? (JR R) ? 5. Help needed for brain processing (Shirley Powell) ? 6. Antibody for the detection of human cells needed (Alexandra Meinl) ? 7. tissue processor (Atoska Gentry) ? 8. Re: Gomeri Iron Stain (Maxim_71@mail.ru) ? 9. Leitz 1512 rotary microtome (sharon.osborn@comcast.net) ? 10. RE: Antibody for the detection of human cells needed ? ? ? (Liz Chlipala) ? 11. Re: H&E quality check (Robert Richmond) ? 12. Re: H&E quality check (Angela Bitting) ? 13. RE: Re: H&E quality check (Michael Mihalik) ? 14. HT position available (LINDA MARGRAF) ? 15. RE: H&E quality check (Weems, Joyce) ? 16. RE: Re: H&E quality check.? ? . (Henry, Charlene) ? 17. RE: Re: H&E quality check.? ? . (Michael Mihalik) ? 18. RE: Gomeri Iron Stain (Tony Henwood) ? 19. Re: H&E quality check. (Dave & Lee Mayhew) ? 20. RE: Re: H&E quality check. (Michael Mihalik) ? 21. Re: Re: H&E quality check. (Dave & Lee Mayhew) ? 22. Re: Re: H&E quality check. . (Anne van Binsbergen) ? 23. Re: H&E quality check (Rene J Buesa) ? 24. RE: Re: H&E quality check. . (Cheri Miller) ---------------------------------------------------------------------- Message: 1 Date: Mon, 7 Jul 2008 11:33:15 -0600 From: "Metzger, Kenneth" Subject: [Histonet] Metal Slide Trays To: Message-ID: ??? Content-Type: text/plain;??? charset="iso-8859-1" Does anyone know where I can order the 20 slot metal slide trays? Thanks, ken Ken Metzger HTL(ASCP) Histology Supervisor ARUP Laboratories 500 Chipeta way Salt Lake City, UT 84108 801.583.2787 ext 3101 - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 ------------------------------ Message: 2 Date: Mon, 7 Jul 2008 10:37:27 -0700 From: "Christine Bark" Subject: [Histonet] RE: H&E quality check To: histonet@lists.utsouthwestern.edu Message-ID: ??? Content-Type: text/plain; charset=us-ascii We check several different slides from our first rack of the day for H&E and cutting quality. Christine Bark HT(ASCP) Senior Histotech, Pathology Saddleback Memorial Medical Center 949-452-3548 cbark@memorialcare.org -----Original Message----- From: "Angela Bitting" Subject: [Histonet] H&E quality check Just curious as to how other hospital labs quality check their H&E slides? Do most review a percentage microscopically? ______________________________________________________________________________ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 3 Date: Mon, 7 Jul 2008 13:40:13 -0400 From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" Subject: RE: [Histonet] Metal Slide Trays To: "Metzger, Kenneth" , ??? histonet@lists.utsouthwestern.edu Message-ID: ??? <1CE1847DFEA0A647B1CCDE4108EA60A7F23C7E@LTA3VS011.ees.hhs.gov> Content-Type: text/plain; charset=us-ascii I think this is what you are looking for.? http://www.daigger.com/catalog/product?deptId=&prodId=16050 Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Metzger, Kenneth Sent: Monday, July 07, 2008 1:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Metal Slide Trays Does anyone know where I can order the 20 slot metal slide trays? Thanks, ken Ken Metzger HTL(ASCP) Histology Supervisor ARUP Laboratories 500 Chipeta way Salt Lake City, UT 84108 801.583.2787 ext 3101 - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Mon, 7 Jul 2008 10:54:49 -0700 From: JR R Subject: [Histonet] Vector Labs Nova HRP--Red Substrate: Not Really ??? EtOH??? compatible? To: Message-ID: Content-Type: text/plain; charset="Windows-1252" The Product information says: "NovaRED? substrate may be dehydrated in ethanol and cleared in organic solvents such as xylene and permanently mounted in a non-aqueous mounting medium such as VectaMount" But I have done two stains now where I initially had intense, specific staining at first--But after my slides come out of the EtOH/Xylene for coverslipping, the staining has vanished. This is getting to be expensive! Has anyone else ever had this problem with Nova Red?? Any ideas? Jerry Ricks Research Scientist University of Washington Department of Pathology _________________________________________________________________ Need to know now? Get instant answers with Windows Live Messenger. http://www.windowslive.com/messenger/connect_your_way.html?ocid=TXT_TAGLM_WL_messenger_072008 ------------------------------ Message: 5 Date: Mon, 07 Jul 2008 14:07:27 -0400 From: Shirley Powell Subject: [Histonet] Help needed for brain processing To: histonet@lists.utsouthwestern.edu Message-ID: <01MWVKZ3Y6Y200ANAP@Macon2.Mercer.edu> Content-Type: text/plain; charset=us-ascii A friend of mine, who has been banned from histonet by a micro-managing employer, needs advice from those who use the microwave to expedite fixation of whole or partial brains for routine processing, or any other rapid means of getting complete fixation on brain. Please send all responses to me personally at this address and I will forward the information to them.? Thanks in advance for your help. Shirley Powell ------------------------------ Message: 6 Date: Mon, 7 Jul 2008 20:24:22 +0200 From: "Alexandra Meinl" Subject: [Histonet] Antibody for the detection of human cells needed To: histonet@lists.utsouthwestern.edu Message-ID: ??? Content-Type: text/plain; charset=UTF-8 Hi all, I'm searching for an antibody for the detection of human cells in mouse tissue. And: we don't know what type of cells we're trying to find. I thought about an ab like a-hu-nuclear membrane or GM 130. Does anybody this? What antibody would you recommend? Any suggestions greatly appreaciated! Alexandra ------------------------------ Message: 7 Date: Mon, 07 Jul 2008 13:35:13 -0500 From: Atoska Gentry Subject: [Histonet] tissue processor To: Histonet Message-ID: <487261E1.3020101@vetmed.auburn.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed hello, please will someone who has info on a modern tissue processor comparable to an obsolete? 25 yr.Autotechnicon Ultra II Tissue Processor please contact me with specifications, product info & pricing ASAP? Thanks, Atoska ------------------------------ Message: 8 Date: Mon, 7 Jul 2008 22:30:20 +0400 From: Maxim_71@mail.ru Subject: Re: [Histonet] Gomeri Iron Stain To: histology@gradymem.org Cc: histonet@lists.utsouthwestern.edu Message-ID: <228377341.20080707223020@mail.ru> Content-Type: text/plain; charset=us-ascii Angie: We never used MWO modification for Gomori Iron Stain. Iron is beautifully can demonsrtate at RT for 10 mins. Vapours of cyanid is very toxic for personnel. Maxim Peshkov Russia, Taganrog. ------------------------------ Message: 9 Date: Mon, 07 Jul 2008 19:11:41 +0000 From: sharon.osborn@comcast.net Subject: [Histonet] Leitz 1512 rotary microtome To: histonet@lists.utsouthwestern.edu Message-ID: ??? <070720081911.18025.48726A6D0006F323000046692215555884029D010D9C01D202019D0E089C@comcast.net> ??? Histonetters, ? ? ? I have a copy of the instructions manual for above referenced microtome should anyone still have this workhorse and need the instructions.? Please send me your contact information and I am happy to send out.? If there is more than one person requesting it, I can make copies. sharon osborn Lab Vision Thermo Fisher Fremont, CA ------------------------------ Message: 10 Date: Mon, 7 Jul 2008 13:20:33 -0600 From: "Liz Chlipala" Subject: RE: [Histonet] Antibody for the detection of human cells ??? needed To: "Alexandra Meinl" , ??? Message-ID: ??? Content-Type: text/plain;??? charset="us-ascii" Alexandra We have tried the human anti-nuclear antibody from chemicon but we did not have much success with it.? We have used a human mitochondrial antibody from Novus with good success but in rat tissues rather than mouse, it's generated in mouse but as long as you use the correct controls it should work.? We have also ran In-situ with a repeat sequence for human DNA and that worked nicely between mouse and also rat and human cells.? We had our probe made by GeneDetect. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Alexandra Meinl Sent: Monday, July 07, 2008 12:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antibody for the detection of human cells needed Hi all, I'm searching for an antibody for the detection of human cells in mouse tissue. And: we don't know what type of cells we're trying to find. I thought about an ab like a-hu-nuclear membrane or GM 130. Does anybody this? What antibody would you recommend? Any suggestions greatly appreaciated! Alexandra _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Mon, 7 Jul 2008 15:34:27 -0400 From: "Robert Richmond" Subject: [Histonet] Re: H&E quality check To: histonet@lists.utsouthwestern.edu Message-ID: ??? Content-Type: text/plain; charset=ISO-8859-1 Well, I've worked in roughly 60 hospital and private lab pathology services in my locum tenens career, and I've never seen a functioning QA (or whatever they call it this year) program for H & E (or any other stain) in a pathology lab. An occasional lab has the pathologist fill out QA sheets telling them that the slides are wonderful - invariably in such labs the slides are horrible. In most labs nobody but the pathologist ever looks at a slide under a microscope. A meaningful QA program would have a pathologist and a senior histotechnologist review some of the day's production, during the working day. I've always been ridiculed for suggesting it. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 12 Date: Mon, 07 Jul 2008 15:47:21 -0400 From: "Angela Bitting" Subject: Re: [Histonet] H&E quality check To: Cc: histonet , ??? histonet-bounces@lists.utsouthwestern.edu Message-ID: <48723A89.2B7F.00C9.0@geisinger.edu> Content-Type: text/plain; charset=US-ASCII Thanks for all of the replies. I guess I need to rephrase my question. I'm wondering how many slides are reviewed daily under the scope for cutting artifacts, incomplete margins, etc. Where I worked before, we checked 10% of the slides under the scope before sending them on to the Pathologists. Do other labs do this? >>> 7/7/2008 9:49 AM >>> Everywhere I have worked there was a liver control slide run daily for Q.C. The techs checked the staining quality before beginning staining for the day. The slide was dated and documented in the log book. Stephanie D. Rivera Safety Assessment Department GlaxoSmithKline 709 Swedeland RD King of Prussia, PA 19406 phone: 610-270-7340 fax: 610-270-7202 "Angela Bitting" Sent by: histonet-bounces@lists.utsouthwestern.edu 07-Jul-2008 07:59 To "histonet" cc Subject [Histonet] H&E quality check Just curious as to how other hospital labs quality check their H&E slides? Do most review a percentage microscopically? IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Mon, 7 Jul 2008 15:50:45 -0400 From: "Michael Mihalik" Subject: RE: [Histonet] Re: H&E quality check To: "'Robert Richmond'" , ??? Message-ID: Content-Type: text/plain;??? charset="US-ASCII" To tag along with this statement.? Wouldn't it be nice if the pathologist could comment on the 'quality' of the stain as they are reviewing the slide in the first place? ...or am I missing something here? Michael Mihalik PathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Monday, July 07, 2008 3:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: H&E quality check Well, I've worked in roughly 60 hospital and private lab pathology services in my locum tenens career, and I've never seen a functioning QA (or whatever they call it this year) program for H & E (or any other stain) in a pathology lab. An occasional lab has the pathologist fill out QA sheets telling them that the slides are wonderful - invariably in such labs the slides are horrible. In most labs nobody but the pathologist ever looks at a slide under a microscope. A meaningful QA program would have a pathologist and a senior histotechnologist review some of the day's production, during the working day. I've always been ridiculed for suggesting it. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Mon, 07 Jul 2008 15:02:20 -0500 From: "LINDA MARGRAF" Subject: [Histonet] HT position available To: Cc: BETHTEWS@HOTMAIL.COM Message-ID: <48722FFC020000DA0002B8BF@CNET3.CHILDRENS.COM> Content-Type: text/plain; charset="iso-8859-15" Here's a message Beth asked me to post for her. Please reply to her if you are interested. Thanks LM (histonet administrator) Technical Applications specialist- laboratory ESSENTIAL DUTIES AND RESPONSIBILITIES Installation of? instrumentation and conducting user training to successfully integrate? products within the customer laboratory. Provide troubleshooting and minor repair to? instrumentation. Work with customers to resolve reagent/staining application issues. Communicate with dispatch and area management on daily/weekly/monthly activities (eg. Salesforce.com) Maintaining tools and test equipment in accordance with Quality procedures Conduct in-service training for customers. Customer follow-up Submit administrative paperwork in a timely and accurate manner. installation forms service work orders expense reports Travel extensively within the region and throughout the country. REQUIRED EDUCATION & EXPERIENCE Associates degree (or equivalent) or higher in Applied Sciences or accredited Medical/Histology Technology course.? Minimum one year general clinical laboratory experience or technical equivalent which may include general histology, cytology, immunohistochemistry, in situ hybridization, biology research or work with automated staining instrumentation. CERTIFICATES, LICENSES, REGISTRATIONS Highly desirable: HISTOLOGY TECHNICIAN American Society of Clinical Pathologists Certification HT or HTL. American Society of Clinical Pathologists Qualification in Immunohistochemistry (QIHC). KNOWLEDGE, SKILLS & ABILITIES Must have the ability to read, analyze, and interpret technical procedures or governmental regulations (CAP, CLIA).? Ability to write reports. Ability to effectively present information and respond to questions from customers.? Ability to calculate antibody dilutions. Ability to interpret a variety of instructions furnished in written, verbal, diagram, or schedule form. WORK ENVIRONMENT? The work environment characteristics described here are representative of those an employee encounters while performing the essential functions of this job traveling to and working within a Clinical Pathology Lab, Research lab, and animal diagnostic lab. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions.? OTHER QUALIFICATIONS Ability to travel. Valid driver*s license. with a clean driving record. COMPENSATION PACKAGE POSITION OFFERS A COMPETITVE BASE SALARY AND A CAR PLUS ALL TRAVEL EXPENSES AND BENEFITS. CONTACT BETH TEWS RECRUITING BETHTEWS@HOTMAIL.COM 623-742-7227 OPENINGS CURRENTLY NEED TO BE FILLED BY 715/08 IN PHOENIX, NYC/NJ,ST LOUIS, BOSTON MORE TO COME SO PLEASE APPLY ??? Please consider the environment before printing this e-mail
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------------------------------ Message: 15 Date: Mon, 7 Jul 2008 16:14:06 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] H&E quality check To: "Angela Bitting" , ??? Cc: histonet , ??? histonet-bounces@lists.utsouthwestern.edu Message-ID: ??? <982A0A9461F9BF438C7B19A6E425A383373163@ITSSSXM01V6.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" We do 1 or 2 per full tray - recording wrinkles, folds, poor staining, etc. to get an approximate 10% reviewed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Monday, July 07, 2008 3:47 PM To: stephanie.d.rivera@gsk.com Cc: histonet; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] H&E quality check Thanks for all of the replies. I guess I need to rephrase my question. I'm wondering how many slides are reviewed daily under the scope for cutting artifacts, incomplete margins, etc. Where I worked before, we checked 10% of the slides under the scope before sending them on to the Pathologists. Do other labs do this? >>> 7/7/2008 9:49 AM >>> Everywhere I have worked there was a liver control slide run daily for Q.C. The techs checked the staining quality before beginning staining for the day. The slide was dated and documented in the log book. Stephanie D. Rivera Safety Assessment Department GlaxoSmithKline 709 Swedeland RD King of Prussia, PA 19406 phone: 610-270-7340 fax: 610-270-7202 "Angela Bitting" Sent by: histonet-bounces@lists.utsouthwestern.edu 07-Jul-2008 07:59 To "histonet" cc Subject [Histonet] H&E quality check Just curious as to how other hospital labs quality check their H&E slides? Do most review a percentage microscopically? IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s).? It may contain information that is privileged and confidential.? Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ------------------------------ Message: 16 Date: Mon, 7 Jul 2008 15:14:08 -0500 From: "Henry, Charlene" Subject: RE: [Histonet] Re: H&E quality check.? ? . To: 'Michael Mihalik' , 'Robert Richmond' ??? , "histonet@lists.utsouthwestern.edu" ??? Message-ID: ??? <03E1F5968F60C5448635D49D38B283ED01460FD918@SJMEMXMBS11.stjude.sjcrh.local> ??? Content-Type: text/plain; charset="us-ascii" Michael, Actually it is now a CAP requirement. ANP.11713 Is there documented evidence of daily review of the technical quality of histologic preparations by the pathologist. This is the way we address this question. Each morning while we are cutting surgical and bone marrow biopsies, we randomly select 2-3 blocks and cut a QC H&E slide in addition to the routine cuts. They are stained along the routine cases and these are given to our Director of AP for review. She then documents the quality of fixation, processing, cutting and staining on a log. While she is on vacation one of our other pathologists review the slides. When the log sheet is full, it is returned to me and filed with our QC records. Charlene Henry HT (ASCP), QIHC Anatomic Pathology Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Monday, July 07, 2008 2:51 PM To: 'Robert Richmond'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: H&E quality check. . To tag along with this statement.? Wouldn't it be nice if the pathologist could comment on the 'quality' of the stain as they are reviewing the slide in the first place? ...or am I missing something here? Michael Mihalik PathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Monday, July 07, 2008 3:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: H&E quality check Well, I've worked in roughly 60 hospital and private lab pathology services in my locum tenens career, and I've never seen a functioning QA (or whatever they call it this year) program for H & E (or any other stain) in a pathology lab. An occasional lab has the pathologist fill out QA sheets telling them that the slides are wonderful - invariably in such labs the slides are horrible. In most labs nobody but the pathologist ever looks at a slide under a microscope. A meaningful QA program would have a pathologist and a senior histotechnologist review some of the day's production, during the working day. I've always been ridiculed for suggesting it. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Mon, 7 Jul 2008 16:47:31 -0400 From: "Michael Mihalik" Subject: RE: [Histonet] Re: H&E quality check.? ? . To: "'Henry, Charlene'" ,??? "'Robert ??? Richmond'" ,??? Message-ID: Content-Type: text/plain;??? charset="US-ASCII" I appreciate the response.? Let me ask this question another way.? Is there anybody who currently logs their histo QC in their computer system, not on paper, not on some department created excel spreadsheet? Would this not be desirable? Michael Mihalik PathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: Henry, Charlene [mailto:Charlene.Henry@STJUDE.ORG] Sent: Monday, July 07, 2008 4:14 PM To: 'Michael Mihalik'; 'Robert Richmond'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: H&E quality check. . Michael, Actually it is now a CAP requirement. ANP.11713 Is there documented evidence of daily review of the technical quality of histologic preparations by the pathologist. This is the way we address this question. Each morning while we are cutting surgical and bone marrow biopsies, we randomly select 2-3 blocks and cut a QC H&E slide in addition to the routine cuts. They are stained along the routine cases and these are given to our Director of AP for review. She then documents the quality of fixation, processing, cutting and staining on a log. While she is on vacation one of our other pathologists review the slides. When the log sheet is full, it is returned to me and filed with our QC records. Charlene Henry HT (ASCP), QIHC Anatomic Pathology Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Monday, July 07, 2008 2:51 PM To: 'Robert Richmond'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: H&E quality check. . To tag along with this statement.? Wouldn't it be nice if the pathologist could comment on the 'quality' of the stain as they are reviewing the slide in the first place? ...or am I missing something here? Michael Mihalik PathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Monday, July 07, 2008 3:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: H&E quality check Well, I've worked in roughly 60 hospital and private lab pathology services in my locum tenens career, and I've never seen a functioning QA (or whatever they call it this year) program for H & E (or any other stain) in a pathology lab. An occasional lab has the pathologist fill out QA sheets telling them that the slides are wonderful - invariably in such labs the slides are horrible. In most labs nobody but the pathologist ever looks at a slide under a microscope. A meaningful QA program would have a pathologist and a senior histotechnologist review some of the day's production, during the working day. I've always been ridiculed for suggesting it. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Tue, 8 Jul 2008 09:19:30 +1000 From: "Tony Henwood" Subject: RE: [Histonet] Gomeri Iron Stain To: "Histology Dept" , ??? Message-ID: Content-Type: text/plain; charset="us-ascii" The following works well though it is a Perl's stain. I can't remember the Gomori Iron stain. The old cranium is shrinking as I get older!! It was developed for frozen sections (eg differentiating melanin from iron pigment) but works quite well for paraffin sections. 1??? Placed ethanol fixed frozen sections or hydrated paraffin section in an the Perl's reagent in a plastic coplin jar (20ml each of 2% Potassium ferrocyanide and 2% HCl). Microwave for 20 sec at 650watts. 2.??? Leave sections in the hot solution for up to 3 minutes, remove if the solution becomes cloudy. 3.??? Rinse in water. 4.??? Counterstain with eosin (as used for the frozen section H&E) or a red nuclear stain (Nuclear Fast Red or Neutral red). 5.??? Rinse in water, DC&M. Reference:??? Henwood (2002) "Microwave Perl's Stain for Urgent Frozen Sections" Aust J Med Sc 23(2):68-69. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Histology Dept Sent: Tuesday, 8 July 2008 1:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gomeri Iron Stain We finally got a microwave for special stains.? Can you use a microwave method to do Gomeri Iron Stain?? If so, would someone please share you procedure with us? Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 19 Date: Mon, 7 Jul 2008 20:45:29 -0400 From: "Dave & Lee Mayhew" Subject: [Histonet] Re: H&E quality check. To: "Histonet" Message-ID: <008601c8e093$eb805690$e013c143@user4ab93baede> Content-Type: text/plain;??? charset="iso-8859-1" Hi Michael, We use Meditech and create a daily QC specimen.? In the data fields for the QC specimen we record all the stains we did for that day, including H&E, and all the specimen numbers that were controlled by those QC slides.? Any problems, concerns, repeats, etc. are recorded in the specimen comment field. A daily report is printed out at the end of the day and filed in the QC binder. Lee Mayhew MLT St. Joseph's Hospital Hamilton? ON? Canada ------------------------------ Message: 20 Date: Mon, 7 Jul 2008 21:16:13 -0400 From: "Michael Mihalik" Subject: RE: [Histonet] Re: H&E quality check. To: "'Dave & Lee Mayhew'" ,??? "'Histonet'" ??? Message-ID: <772604D2DC974271BCC24D75ED3FA5ED@MDMM1330> Content-Type: text/plain;??? charset="US-ASCII" So you create a 'normal' case where the specimen is a qc case and then fill in all the 'normal' fields with qc data. Is that correct? ....or are there specialized qc data fields, like a prompt for stain quality, block quality, etc. I'm looking to see if any computer system does anything special for QC purposes, relative to block, slides, stains, etc. Michael Mihalik PathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dave & Lee Mayhew Sent: Monday, July 07, 2008 8:45 PM To: Histonet Subject: [Histonet] Re: H&E quality check. Hi Michael, We use Meditech and create a daily QC specimen.? In the data fields for the QC specimen we record all the stains we did for that day, including H&E, and all the specimen numbers that were controlled by those QC slides.? Any problems, concerns, repeats, etc. are recorded in the specimen comment field. A daily report is printed out at the end of the day and filed in the QC binder. Lee Mayhew MLT St. Joseph's Hospital Hamilton? ON? Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Mon, 7 Jul 2008 21:34:08 -0400 From: "Dave & Lee Mayhew" Subject: Re: [Histonet] Re: H&E quality check. To: "Michael Mihalik" ,??? "'Histonet'" ??? Message-ID: <00a501c8e09a$b7876bb0$e013c143@user4ab93baede> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; ??? reply-type=original Michael The case created is a special QC Specimen.? Meditech allows you to create any data fields that you want, they do not have to be the same data sections as are found in patient specimens, so you could create a prompt for the things you suggest if you wanted to.? The workload that you enter into this specimen is counted as quality control not patient workload. I should say that we also have a daily sheet that the day's pathologist fills out to document any of their concerns.? We do not enter this into Meditech, although we, or they, could. ? Lee Mayhew MLT > St. Joseph's Hospital > Hamilton? ON? Canada ------------------------------ Message: 22 Date: Tue, 8 Jul 2008 08:52:10 +0400 From: "Anne van Binsbergen" Subject: Re: [Histonet] Re: H&E quality check. . To: "Michael Mihalik" Cc: histonet@lists.utsouthwestern.edu, "Henry,??? Charlene" ??? , Robert Richmond Message-ID: ??? Content-Type: text/plain; charset=ISO-8859-1 we have a tissue array (tonsil, kidney, skin, L/N, appendix) block which we made ourselves by using a dermatology punch tool identical slides are cut and kept in a box each morning we stain one and it gets viewed by a senior tech - the path does not have time for such matters!! results logged, adjustments noted, signed off, logs filed, slides filed - all ready for CAP to scrutinise if we adjust the stain (or anything else) we run another QC slide and relog the results we are in the process of making an extended array for a whole bunch of specials too its a pain in the @$$ but my staff are used to the process now ideally it should be done the 'samurai' way - but im just the tech - what do i know!!! ;)) annieinarabia 2008/7/8 Michael Mihalik : > I appreciate the response.? Let me ask this question another way.? Is there > anybody who currently logs their histo QC in their computer system, not on > paper, not on some department created excel spreadsheet? > > Would this not be desirable? > > > Michael Mihalik > PathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540 > | fax: 270.423.0968 > > > > > -----Original Message----- >? From: Henry, Charlene [mailto:Charlene.Henry@STJUDE.ORG] > Sent: Monday, July 07, 2008 4:14 PM > To: 'Michael Mihalik'; 'Robert Richmond'; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: H&E quality check. . > > Michael, > Actually it is now a CAP requirement. > > ANP.11713 Is there documented evidence of daily review of the technical > quality of histologic preparations by the pathologist. > > This is the way we address this question. > Each morning while we are cutting surgical and bone marrow biopsies, we > randomly select 2-3 blocks and cut a QC H&E slide in addition to the > routine > cuts. They are stained along the routine cases and these are given to our > Director of AP for review. She then documents the quality of fixation, > processing, cutting and staining on a log. While she is on vacation one of > our other pathologists review the slides. When the log sheet is full, it is > returned to me and filed with our QC records. > > > Charlene Henry HT (ASCP), QIHC > Anatomic Pathology Section Head > Department of Pathology > St. Jude Children's Research Hospital > 901-495-3191 > fax 901-495-3100 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael > Mihalik > Sent: Monday, July 07, 2008 2:51 PM > To: 'Robert Richmond'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: H&E quality check. . > > To tag along with this statement.? Wouldn't it be nice if the pathologist > could comment on the 'quality' of the stain as they are reviewing the slide > in the first place? > > ...or am I missing something here? > > Michael Mihalik > PathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540 > | fax: 270.423.0968 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert > Richmond > Sent: Monday, July 07, 2008 3:34 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: H&E quality check > > Well, I've worked in roughly 60 hospital and private lab pathology services > in my locum tenens career, and I've never seen a functioning QA (or > whatever > they call it this year) program for H & E (or any other stain) in a > pathology lab. An occasional lab has the pathologist fill out QA sheets > telling them that the slides are wonderful - invariably in such labs the > slides are horrible. In most labs nobody but the pathologist ever looks at > a > slide under a microscope. > > A meaningful QA program would have a pathologist and a senior > histotechnologist review some of the day's production, during the working > day. I've always been ridiculed for suggesting it. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE ------------------------------ Message: 23 Date: Tue, 8 Jul 2008 04:46:40 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] H&E quality check To: stephanie.d.rivera@gsk.com, Angela Bitting ??? Cc: histonet , ??? histonet-bounces@lists.utsouthwestern.edu Message-ID: <661700.14357.qm@web65702.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 If you get to a percentage, then you will waste your whole day doing that. What I used to do is to let the pathologists reject the slides, then I reviewed them, found out who cut them, discuss the issue, fill the QA and retrain the HT who did the bad slide.Any other approach will be a total loss of precious time.Don't even think if giving a form to the pathologists to fill out the problem they found, they are not going to do it, and if they do, they will be wasting their time. That is the supervisor's task, the PT task is to reject, the supervisor's task is to prevent the problem from occurring again. Ren? J. --- On Mon, 7/7/08, Angela Bitting wrote: ? ? ? ? ------------------------------ Message: 24 Date: Tue, 8 Jul 2008 07:05:20 -0500 From: "Cheri Miller" Subject: RE: [Histonet] Re: H&E quality check. . To: "'Anne van Binsbergen'" ,??? "'Michael Mihalik'" ??? Cc: histonet@lists.utsouthwestern.edu, "'Henry,??? Charlene'" ??? ,??? 'Robert Richmond' Message-ID: <000901c8e0f2$e4e24280$3d02a8c0@plab.local> Content-Type: text/plain;??? charset="us-ascii" We check the slide quality daily by using a control slide (usually an appendix or gallbladder) with the first rack of slides. I then check random slides from each rack stained, we also have a QA sheet the paths fill out daily. They grade us on grossing, specimen sampling, processing, embedding, microtomy, staining and cover slipping. Most of them see this as a true quality control indicator and let us know when we have an issue; a few just fill it out without any real critiquing, which defeats the whole purpose.? Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message.? If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information.? Any dissemination, distribution, or copying of this e-mail is strictly prohibited.? If you have received this message in error, please notify the sender immediately and delete this email from your system. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 56, Issue 9 *************************************** From RSRICHMOND <@t> aol.com Tue Jul 8 08:11:51 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Tue Jul 8 08:11:57 2008 Subject: [Histonet] Re: Gomori (rather Perls) iron stain Message-ID: Angie Barnett at Grady Memorial Hospital in Atlanta (hey, I'm up the road at Blairsville this week) leads to some questions about the iron stain. The usual "stain" for stainable iron (hemosiderin mostly, not the iron within erythrocytes) results in deposition of a dense blue pigment, ferric ferrocyanide (Prussian blue). This method was introduced by Max Perls in 1846, and is the oldest histochemical technique still in use. I annotate iron stains as "the Perls Prussian blue reaction demonstrates only small amounts of stainable iron". George Gomori (1904-1957) is sort of the founder of modern histochemistry. Born in Budapest, he spent most of his active career at the University of Chicago. (Google his name to find a superb biography on the Rootsweb site, complete with a photomike of a GMS stain showing fungi in a fine case of jock itch.) Customarily pronounced "GOMmery" in English, and I don't know how in Hungarian. Gomori wrote widely on histochemical topics, including iron staining, and his name was often attached to techniques he really had nothing to do with, in addition to the numerous ones he introduced. He's a bit before my time in medicine - does anyone on this list remember him? Bob Richmond Samurai Pathologist Knoxville TN From Jerry <@t> ralambusa.com Tue Jul 8 08:23:44 2008 From: Jerry <@t> ralambusa.com (Jerry Helisek) Date: Tue Jul 8 08:23:48 2008 Subject: [Histonet] RE: Histonet Digest, Vol 56, Issue 9 Message-ID: <3855F92002259948A66A8CA2D16E3A4F0B56A7@server.ralambusa.com> Ken, Are you looking for aluminum slide trays? If so, see: http://www.ralamb.net/product_info.php?products_id=428 But if you are looking for cabinets this may be what you are looking for: (there are a few choices listed) http://www.ralamb.net/advanced_search_result.php?keywords=cabinet Good luck hunting! Have a great day! Also, MarketLab, LabStorage, PSL and Electron Microscopy may be good outlets for you as well. ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ ---------------------------------------------------------------------- Message: 1 Date: Mon, 7 Jul 2008 11:33:15 -0600 From: "Metzger, Kenneth" Subject: [Histonet] Metal Slide Trays To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Does anyone know where I can order the 20 slot metal slide trays? Thanks, ken Ken Metzger HTL(ASCP) Histology Supervisor ARUP Laboratories 500 Chipeta way Salt Lake City, UT 84108 801.583.2787 ext 3101 - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From snow12 <@t> comcast.net Tue Jul 8 08:37:11 2008 From: snow12 <@t> comcast.net (snow12@comcast.net) Date: Tue Jul 8 08:37:17 2008 Subject: [Histonet] THE IRON STAIN FOR ASBESTOS BODIES Message-ID: <070820081337.29460.48736D87000C81C5000073142215567074CDCE9901029C@comcast.net> Greetings fellow histonetters: I currently stain slides (FE STAIN) for a forensic pathologist to document asbestos bodies. Does any one know any other staining methods for asbestos bodies besides an iron stain? Is an Antibody available for an asbestos body or perhaps the asbestos body coating? I would prefer to stay away from the digestion filter technique however does anyone have a procedure using the digestion filter technique I would appreciate it. Jonathan R Dyke, AAS, HT (ASCP) Diagnostics Pathology Consultants SNOW12@comcast.net From MITCHELLJA <@t> email.chop.edu Tue Jul 8 08:37:25 2008 From: MITCHELLJA <@t> email.chop.edu (Janice Mitchell) Date: Tue Jul 8 08:38:19 2008 Subject: [Histonet] formalin Message-ID: Has anyone out there is histo land tried the tissuetek VIP fix? It is suppose to be neutral ph, therefore doing away with the need for hot water washes. Janice From ian.montgomery <@t> bio.gla.ac.uk Tue Jul 8 09:04:42 2008 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Tue Jul 8 09:04:47 2008 Subject: [Histonet] Antibody advice. Message-ID: <21863CACA777438880F1524B048E04CC@IBLS.GLA.AC.UK> Co-worker wants to look at macrophage using F4/80, neutrophils using Ly-6 and T-cells with CD3 and CD4. Any suggestions for a reliable source of the antibodies? Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. From ht.ascp <@t> yahoo.com Tue Jul 8 09:05:43 2008 From: ht.ascp <@t> yahoo.com (Jon Google) Date: Tue Jul 8 09:05:55 2008 Subject: [Histonet] H&E quality check Message-ID: <449905.69284.qm@web59905.mail.ac4.yahoo.com> Would it not be better to check the slides that go out for quality and recutting as needed? Waiting for the pathologist to reject the slide could add quite a bit of time to the turn around and even delay the case to the next day. Taking?two minutes to look over a tray and check 2 under the scope (10%) is not that bad. And if you are doing it while you are block checking, it does not really even add time. It can be done in the small amount of time in between racks.? What happens if your techs are rotating the solutions on the stainer and mix up the reagents?? If you dont continually check your slides, you will not notice until your pathologist returns a batch. Then it makes the lab look incompetant for putting out poor stains. ? Quality improvement should be your goal, not just churning out slides and waiting for rejects. --- On Tue, 7/8/08, Rene J Buesa wrote: From: Rene J Buesa Subject: Re: [Histonet] H&E quality check To: stephanie.d.rivera@gsk.com, "Angela Bitting" Cc: "histonet" , histonet-bounces@lists.utsouthwestern.edu Date: Tuesday, July 8, 2008, 11:46 AM If you get to a percentage, then you will waste your whole day doing that. What I used to do is to let the pathologists reject the slides, then I reviewed them, found out who cut them, discuss the issue, fill the QA and retrain the HT who did the bad slide.Any other approach will be a total loss of precious time.Don't even think if giving a form to the pathologists to fill out the problem they found, they are not going to do it, and if they do, they will be wasting their time. That is the supervisor's task, the PT task is to reject, the supervisor's task is to prevent the problem from occurring again. Ren? J. --- On Mon, 7/7/08, Angela Bitting wrote: ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Tue Jul 8 09:20:20 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Jul 8 09:20:27 2008 Subject: [Histonet] Antibody advice. In-Reply-To: <21863CACA777438880F1524B048E04CC@IBLS.GLA.AC.UK> References: <21863CACA777438880F1524B048E04CC@IBLS.GLA.AC.UK> Message-ID: Ian I'm assuming that you want to stain mouse tissue. This is what we use: F4/80 from serotec cat: MCAP497 (paraffin) Neutrophil from serotec cat: MCA771 clone clone 7/4 (paraffin) CD3 from dako cat: A0452 (paraffin) CD4 from BD Pharmagen cat: 550278 (only works on frozen sections) Hope this helps. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian Montgomery Sent: Tuesday, July 08, 2008 8:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Antibody advice. Co-worker wants to look at macrophage using F4/80, neutrophils using Ly-6 and T-cells with CD3 and CD4. Any suggestions for a reliable source of the antibodies? Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ploykasek <@t> phenopath.com Tue Jul 8 09:37:34 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue Jul 8 09:38:05 2008 Subject: [Histonet] Antibody advice. In-Reply-To: <21863CACA777438880F1524B048E04CC@IBLS.GLA.AC.UK> Message-ID: Ian, Is this for formalin fixed, paraffin embedded human tissue? I am not familiar with clone F4/80 for macrophages, but we recently added CD163 mouse clone 10D6 that works well for monos/macs (better than CD68). For CD3 I like mouse clone PS1, and for CD4 mouse clone 4B12 works best for us. CD4 can be a bit tricky out of paraffin, but this clone works well. Hope this helps. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Co-worker wants to look at macrophage using F4/80, neutrophils > using Ly-6 and T-cells with CD3 and CD4. Any suggestions for a reliable > source of the antibodies? > > Ian. > > > > > > Dr. Ian Montgomery, > > Histotechnology, > > I.B.L.S. Support Unit, > > Thomson Building, > > University of Glasgow, > > Glasgow, > > G12 8QQ. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From c.m.vanderloos <@t> amc.uva.nl Tue Jul 8 09:50:03 2008 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Tue Jul 8 09:50:20 2008 Subject: [Histonet] RE: Vector Labs Nova HRP--Red Substrate: Not Really EtOH compatible? Message-ID: Jerry,The reaction product from Vector NovaRed gradually dissolves in water. Are you sure your slides didn't stay in water too long? Are your slides completely dehydrated before mounting?We use VNRed a lot and uses the following approach: After developing the red color, wash with distilled water for 2 min. Dry slides at 50C hot plate. Coverslip with VectaMount and leave slides at the hot plate for 1 hour. This preserves the red reaction product very well. If you immerse the slides in alcohols, the red color shifts a bit towards brown. Especially with double staining we don't want that.Hope this helps a bit.ChrisChris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Mon, 7 Jul 2008 10:54:49 -0700 From: JR R Subject: [Histonet] Vector Labs Nova HRP--Red Substrate: Not Really EtOH compatible? To: histonet@lists.utsouthwestern.edu The Product information says: "NovaRED? substrate may be dehydrated in ethanol and cleared in organic solvents such as xylene and permanently mounted in a non-aqueous mounting medium such as VectaMount" But I have done two stains now where I initially had intense, specific staining at first--But after my slides come out of the EtOH/Xylene for coverslipping, the staining has vanished. This is getting to be expensive! Has anyone else ever had this problem with Nova Red? Any ideas? Jerry Ricks Research Scientist University of Washington Department of Pathology From anh2006 <@t> med.cornell.edu Tue Jul 8 09:56:08 2008 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Tue Jul 8 09:56:22 2008 Subject: [Histonet] Antibody advice. Message-ID: F4/80 from Serotec works very well in FFPE sections, haven't tried in frozen CD4 from BD Pharmingen is beautiful in frozens, I understand it doesn't work in FFPE CD3 from BD Pharmingen is beautiful in frozens, I understand it DOES work in FFPE Ly-6/Gr-1 from BD Pharmingen is beautiful in frozens, I haven't tested in FFPE > Co-worker wants to look at macrophage using F4/80, neutrophils > using Ly-6 and T-cells with CD3 and CD4. Any suggestions for a reliable > source of the antibodies? > > Ian. > > > > > > Dr. Ian Montgomery, > > Histotechnology, > > I.B.L.S. Support Unit, > > Thomson Building, > > University of Glasgow, > > Glasgow, -- From gu.lang <@t> gmx.at Tue Jul 8 10:01:54 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Jul 8 10:02:17 2008 Subject: AW: [Histonet] H&E quality check In-Reply-To: <661700.14357.qm@web65702.mail.ac4.yahoo.com> Message-ID: <0402798811164941817A9404B958C1CB@dielangs.at> I am with Ren?. The slides rejected are for our lab 0,001 slides of all cut slides per year. The additional time for those few slides is acceptable. The only cause to reject a slide is the impossibility to read it correctly, or if the orientation of the tissue is false. By the way. I like to check the HE stain without the microscope. If it is too blue or too red, you see it from a distance. ;) Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Rene J Buesa Gesendet: Dienstag, 08. Juli 2008 13:47 An: stephanie.d.rivera@gsk.com; Angela Bitting Cc: histonet; histonet-bounces@lists.utsouthwestern.edu Betreff: Re: [Histonet] H&E quality check If you get to a percentage, then you will waste your whole day doing that. What I used to do is to let the pathologists reject the slides, then I reviewed them, found out who cut them, discuss the issue, fill the QA and retrain the HT who did the bad slide.Any other approach will be a total loss of precious time.Don't even think if giving a form to the pathologists to fill out the problem they found, they are not going to do it, and if they do, they will be wasting their time. That is the supervisor's task, the PT task is to reject, the supervisor's task is to prevent the problem from occurring again. Ren? J. --- On Mon, 7/7/08, Angela Bitting wrote: ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr <@t> comcast.net Tue Jul 8 10:22:01 2008 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Tue Jul 8 10:22:19 2008 Subject: [Histonet] antibodies Message-ID: <070820081522.11460.48738619000161FF00002CC422007504389D09020704040A0105@comcast.net> Ian, just curious, Were you asking about murine or human F4-80, Ly-6, 3 and 4? Seeing F4-80 (which is a mouse molecule target although there are human equivalents for macs) and Ly-6 which generally you think of in mouse terms (although the human equivalent family of molecules has been described), I was wondering if this is for human or mouse tissues? Ray Koelling PhenoPath Labs Seattle, WA From contact <@t> excaliburpathology.com Tue Jul 8 10:31:12 2008 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Tue Jul 8 10:31:16 2008 Subject: [Histonet] gutterguard Message-ID: <869375.6562.qm@web50108.mail.re2.yahoo.com> ?Hi Margaret, I found mine at Ace Hardware. I also use it to make baskets of all sizes for processing. Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 631 N. Broadway Ave. Moore, OK 73160 405-759-3953 contact@excaliburpathology.com www.excaliburpathology.com From ht.ascp <@t> yahoo.com Tue Jul 8 11:32:01 2008 From: ht.ascp <@t> yahoo.com (Jon Google) Date: Tue Jul 8 11:32:06 2008 Subject: AW: [Histonet] H&E quality check In-Reply-To: <0402798811164941817A9404B958C1CB@dielangs.at> Message-ID: <701357.15100.qm@web59904.mail.ac4.yahoo.com> If it is all about time, the pathologist's time is more important.? If it takes them longer to read the slide because of cutting artifact, then that is more of a waste.? The lab should take the time to turn out a high quality slide, and ensure the ongoing quality throughout the day. Quicker screening and easier screening by the pathologists will lead to better TAT and less misdiagnosis. --- On Tue, 7/8/08, Gudrun Lang wrote: From: Gudrun Lang Subject: AW: [Histonet] H&E quality check To: histonet@lists.utsouthwestern.edu Date: Tuesday, July 8, 2008, 3:01 PM I am with Ren?. The slides rejected are for our lab 0,001 slides of all cut slides per year. The additional time for those few slides is acceptable. The only cause to reject a slide is the impossibility to read it correctly, or if the orientation of the tissue is false. By the way. I like to check the HE stain without the microscope. If it is too blue or too red, you see it from a distance. ;) Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Rene J Buesa Gesendet: Dienstag, 08. Juli 2008 13:47 An: stephanie.d.rivera@gsk.com; Angela Bitting Cc: histonet; histonet-bounces@lists.utsouthwestern.edu Betreff: Re: [Histonet] H&E quality check If you get to a percentage, then you will waste your whole day doing that. What I used to do is to let the pathologists reject the slides, then I reviewed them, found out who cut them, discuss the issue, fill the QA and retrain the HT who did the bad slide.Any other approach will be a total loss of precious time.Don't even think if giving a form to the pathologists to fill out the problem they found, they are not going to do it, and if they do, they will be wasting their time. That is the supervisor's task, the PT task is to reject, the supervisor's task is to prevent the problem from occurring again. Ren? J. --- On Mon, 7/7/08, Angela Bitting wrote: ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Erin.Martin <@t> ucsf.edu Tue Jul 8 12:04:45 2008 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Tue Jul 8 12:06:11 2008 Subject: [Histonet] VZV antibody Message-ID: Hi everyone, I am having a really hard time getting the Varicella Zoster Virus antibody from Novocastra to work. If anyone is currently using this, would you mind sharing your procedure? Thanks, Erin From gentras <@t> vetmed.auburn.edu Tue Jul 8 12:40:28 2008 From: gentras <@t> vetmed.auburn.edu (Atoska Gentry) Date: Tue Jul 8 12:40:34 2008 Subject: [Fwd: [Histonet] tissue processor], addendnum Message-ID: <4873A68C.7000108@vetmed.auburn.edu> Hello All, perhaps I did not include enough info in my initial inquiry. We don't use the conventional / standard cassettes for processing. We embed with "L" shapes because our tissues vary in size. Also, we process in stainless steel circular cassettes both large and small; the large cassettes are 3" in diameter. And since my PI wants to maintain the same processing & embedding method we will need something which can accommodate our current processing/embedding method. These are the reasons I requested info on a processor comparable to the Autotechnicon Ultra II. Also, it needs to be able to run 4 - 22.5 hour process cycle. Although, we process extraneural sections our primary area of interest is CNS. Thanks very much. Atoska From koswalt <@t> deltapathology.com Tue Jul 8 12:53:05 2008 From: koswalt <@t> deltapathology.com (koswalt@deltapathology.com) Date: Tue Jul 8 12:53:20 2008 Subject: [Histonet] Looking for ALK-1 control tissue Message-ID: <20080708125305.34hcpth628swckww@mail3.dpgdomain.com> I have been searching in vain to locate ALK-1 positive control tissue for IHC. If anyone has any suggestions please let me know. We have tried the in-house method and have found only one block with a very small amount of tissue left in it. I have gone back many years for this. Thanks, Kim From ruebenjcarter <@t> gmail.com Tue Jul 8 13:06:49 2008 From: ruebenjcarter <@t> gmail.com (R C) Date: Tue Jul 8 13:06:57 2008 Subject: [Histonet] Re:H&E quality check Message-ID: <2a926e3f0807081106k23393ca4k3024b34568539cb8@mail.gmail.com> It has been my experience that mistakes are contained and corrected in the histology lab, and forward only quality work to the Pathologists. This allows time to be categorized as beneficial. Ruben Carter On Tue, Jul 8, 2008 at 10:01 AM, wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Antibody advice. (Andrea Hooper) > 2. AW: [Histonet] H&E quality check (Gudrun Lang) > 3. antibodies (koellingr@comcast.net) > 4. gutterguard (Paula Pierce) > 5. Re: AW: [Histonet] H&E quality check (Jon Google) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 08 Jul 2008 10:56:08 -0400 > From: "Andrea Hooper" > Subject: Re: [Histonet] Antibody advice. > To: Histonet > Message-ID: > Content-Type: text/plain; charset=us-ascii; format=flowed > > F4/80 from Serotec works very well in FFPE sections, haven't tried in > frozen > CD4 from BD Pharmingen is beautiful in frozens, I understand it > doesn't work in FFPE > CD3 from BD Pharmingen is beautiful in frozens, I understand it DOES > work in FFPE > Ly-6/Gr-1 from BD Pharmingen is beautiful in frozens, I haven't tested in > FFPE > > > Co-worker wants to look at macrophage using F4/80, neutrophils > > using Ly-6 and T-cells with CD3 and CD4. Any suggestions for a reliable > > source of the antibodies? > > > > Ian. > > > > > > > > > > > > Dr. Ian Montgomery, > > > > Histotechnology, > > > > I.B.L.S. Support Unit, > > > > Thomson Building, > > > > University of Glasgow, > > > > Glasgow, > > -- > > > > ------------------------------ > > Message: 2 > Date: Tue, 8 Jul 2008 17:01:54 +0200 > From: "Gudrun Lang" > Subject: AW: [Histonet] H&E quality check > To: > Message-ID: <0402798811164941817A9404B958C1CB@dielangs.at> > Content-Type: text/plain; charset="iso-8859-1" > > I am with Ren?. The slides rejected are for our lab 0,001 slides of all cut > slides per year. The additional time for those few slides is acceptable. > The > only cause to reject a slide is the impossibility to read it correctly, or > if the orientation of the tissue is false. > By the way. I like to check the HE stain without the microscope. If it is > too blue or too red, you see it from a distance. ;) > > Gudrun Lang > > Biomed. Analytikerin > Histolabor > Akh Linz > Krankenhausstr. 9 > 4020 Linz > +43(0)732/7806-6754 > > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Rene J > Buesa > Gesendet: Dienstag, 08. Juli 2008 13:47 > An: stephanie.d.rivera@gsk.com; Angela Bitting > Cc: histonet; histonet-bounces@lists.utsouthwestern.edu > Betreff: Re: [Histonet] H&E quality check > > If you get to a percentage, then you will waste your whole day doing that. > What I used to do is to let the pathologists reject the slides, then I > reviewed them, found out who cut them, discuss the issue, fill the QA and > retrain the HT who did the bad slide.Any other approach will be a total > loss > of precious time.Don't even think if giving a form to the pathologists to > fill out the problem they found, they are not going to do it, and if they > do, they will be wasting their time. That is the supervisor's task, the PT > task is to reject, the supervisor's task is to prevent the problem from > occurring again. > Ren? J. > > --- On Mon, 7/7/08, Angela Bitting wrote: > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 3 > Date: Tue, 08 Jul 2008 15:22:01 +0000 > From: koellingr@comcast.net > Subject: [Histonet] antibodies > To: ian.montgomery@bio.gla.ac.uk>, Message-ID: > < > 070820081522.11460.48738619000161FF00002CC422007504389D09020704040A0105@comcast.net > > > > > Ian, just curious, > > Were you asking about murine or human F4-80, Ly-6, 3 and 4? Seeing F4-80 > (which is a mouse molecule target although there are human equivalents for > macs) and Ly-6 which generally you think of in mouse terms (although the > human equivalent family of molecules has been described), I was wondering if > this is for human or mouse tissues? > > Ray Koelling > PhenoPath Labs > Seattle, WA > > > > ------------------------------ > > Message: 4 > Date: Tue, 8 Jul 2008 08:31:12 -0700 (PDT) > From: Paula Pierce > Subject: [Histonet] gutterguard > To: Histonet > Message-ID: <869375.6562.qm@web50108.mail.re2.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > > Hi Margaret, > I found mine at Ace Hardware. I also use it to make baskets of all sizes > for processing. Paula Pierce, HTL(ASCP)HT > Excalibur Pathology, Inc. > 631 N. Broadway Ave. > Moore, OK 73160 > 405-759-3953 > contact@excaliburpathology.com > www.excaliburpathology.com > > ------------------------------ > > Message: 5 > Date: Tue, 8 Jul 2008 09:32:01 -0700 (PDT) > From: Jon Google > Subject: Re: AW: [Histonet] H&E quality check > To: histonet@lists.utsouthwestern.edu > Message-ID: <701357.15100.qm@web59904.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > If it is all about time, the pathologist's time is more important. If it > takes them longer to read the slide because of cutting artifact, then that > is more of a waste. The lab should take the time to turn out a high quality > slide, and ensure the ongoing quality throughout the day. Quicker screening > and easier screening by the pathologists will lead to better TAT and less > misdiagnosis. > > --- On Tue, 7/8/08, Gudrun Lang wrote: > > From: Gudrun Lang > Subject: AW: [Histonet] H&E quality check > To: histonet@lists.utsouthwestern.edu > Date: Tuesday, July 8, 2008, 3:01 PM > > I am with Ren?. The slides rejected are for our lab 0,001 slides of all cut > slides per year. The additional time for those few slides is acceptable. > The > only cause to reject a slide is the impossibility to read it correctly, or > if the orientation of the tissue is false. > By the way. I like to check the HE stain without the microscope. If it is > too blue or too red, you see it from a distance. ;) > > Gudrun Lang > > Biomed. Analytikerin > Histolabor > Akh Linz > Krankenhausstr. 9 > 4020 Linz > +43(0)732/7806-6754 > > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Rene J > Buesa > Gesendet: Dienstag, 08. Juli 2008 13:47 > An: stephanie.d.rivera@gsk.com; Angela Bitting > Cc: histonet; histonet-bounces@lists.utsouthwestern.edu > Betreff: Re: [Histonet] H&E quality check > > If you get to a percentage, then you will waste your whole day doing that. > What I used to do is to let the pathologists reject the slides, then I > reviewed them, found out who cut them, discuss the issue, fill the QA and > retrain the HT who did the bad slide.Any other approach will be a total > loss > of precious time.Don't even think if giving a form to the pathologists to > fill out the problem they found, they are not going to do it, and if they > do, they will be wasting their time. That is the supervisor's task, the PT > task is to reject, the supervisor's task is to prevent the problem from > occurring again. > Ren? J. > > --- On Mon, 7/7/08, Angela Bitting wrote: > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 56, Issue 11 > **************************************** > From AnthonyH <@t> chw.edu.au Tue Jul 8 17:55:03 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Jul 8 17:55:17 2008 Subject: [Histonet] THE IRON STAIN FOR ASBESTOS BODIES In-Reply-To: <070820081337.29460.48736D87000C81C5000073142215567074CDCE9901029C@comcast.net> Message-ID: The following works quite well: USE OF CELLULOSE MEMBRANE FILTERS TO DETECT ASBESTOS IN SPUTUM SPECIMENS REFERENCE: Pelosi et al (1990) Acta Cytolog 34(4):588-590. PRINCIPLE: Pelosi et al (1990) found that the total number of asbestos bodies and fibres per specimen was twice as high on the membrane filters as on conventionally stained PAP smears. The Perl's stain allows one to identify only the coated fibres (asbestos bodies and/or true ferruginous bodies) not the uncoated fibres. Not all ferruginous bodies have an asbestos fibre core (eg fibrous glass, aluminium silicate, silicon carbide, talc, titanium oxide and erionite) but can give a positive Perl's reaction. They lack an asbestos fibre core. SOLUTIONS: 1. 10% Sodium Hypochlorite (7% active chlorine) METHOD: 1. Dissolve sputum in 10-15ml 10% sodium hypochlorite. 2. Filter resultant solution using positive pressure (syringe technique) onto a cellulose nitrate membrane. 3. Examine filters under the microscope. RESULTS: Only bodies with typical dumbell, javelin or segmented morphologies and thin optically transparent cores are counted as asbestos bodies. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of snow12@comcast.net Sent: Tuesday, 8 July 2008 11:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] THE IRON STAIN FOR ASBESTOS BODIES Greetings fellow histonetters: I currently stain slides (FE STAIN) for a forensic pathologist to document asbestos bodies. Does any one know any other staining methods for asbestos bodies besides an iron stain? Is an Antibody available for an asbestos body or perhaps the asbestos body coating? I would prefer to stay away from the digestion filter technique however does anyone have a procedure using the digestion filter technique I would appreciate it. Jonathan R Dyke, AAS, HT (ASCP) Diagnostics Pathology Consultants SNOW12@comcast.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From hyters <@t> onid.orst.edu Tue Jul 8 21:50:17 2008 From: hyters <@t> onid.orst.edu (hyters@onid.orst.edu) Date: Tue Jul 8 21:50:23 2008 Subject: [Histonet] Single-cell susension from tissue Message-ID: <20080708195017.dybvkj73i8swo4og@webmail.oregonstate.edu> hello, having trouble fully dissociating dermal tissue to use in a cell count on a hemacytometer. Can I vortex cells in suspension or should I just keep them in the enzyme bath a bit longer? (worried about surface markers for FACS) thanks for any help, stephen From marktarango <@t> gmail.com Tue Jul 8 22:43:24 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Tue Jul 8 22:43:29 2008 Subject: [Histonet] Single-cell susension from tissue In-Reply-To: <20080708195017.dybvkj73i8swo4og@webmail.oregonstate.edu> References: <20080708195017.dybvkj73i8swo4og@webmail.oregonstate.edu> Message-ID: <5b6eb13e0807082043s1ec688e1j485213bff1984bea@mail.gmail.com> Hi Stephen You can vortex but I'd keep them in the enzyme bath longer. If you're worried about losing a population of cells (or the epitopes getting chewed up from digestion), remove the cells that have come out of the tissue and hold them in PBS. You can put the remaining tissue back for digestion (you don't want to exclude a population of cells because they never came out of the tissue). Different populations of cells can come out at different times and you can even end up selecting out populations based on how fast you are centrifuging. Mark On Tue, Jul 8, 2008 at 7:50 PM, wrote: > hello, having trouble fully dissociating dermal tissue to use in a cell > count on a hemacytometer. Can I vortex cells in suspension or should I just > keep them in the enzyme bath a bit longer? (worried about surface markers > for FACS) > > thanks for any help, > stephen > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From anh2006 <@t> med.cornell.edu Tue Jul 8 23:06:38 2008 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Tue Jul 8 23:09:19 2008 Subject: [Histonet] Single-cell susension from tissue In-Reply-To: <5b6eb13e0807082043s1ec688e1j485213bff1984bea@mail.gmail.com> References: <20080708195017.dybvkj73i8swo4og@webmail.oregonstate.edu><5b6eb13e0807082043s1ec688e1j485213bff1984bea@mail.gmail.com> Message-ID: <1504269054-1215576553-cardhu_decombobulator_blackberry.rim.net-622599549-@bxe157.bisx.prod.on.blackberry> What enzymes are you using for digestion? Just curious .... Have you looked into the BD Medimachines for cell dissociation? They work great .... So easy! -----Original Message----- From: Mark Tarango Date: Tue, 08 Jul 2008 20:43:24 To: Cc: Subject: Re: [Histonet] Single-cell susension from tissue Hi Stephen You can vortex but I'd keep them in the enzyme bath longer. If you're worried about losing a population of cells (or the epitopes getting chewed up from digestion), remove the cells that have come out of the tissue and hold them in PBS. You can put the remaining tissue back for digestion (you don't want to exclude a population of cells because they never came out of the tissue). Different populations of cells can come out at different times and you can even end up selecting out populations based on how fast you are centrifuging. Mark On Tue, Jul 8, 2008 at 7:50 PM, wrote: > hello, having trouble fully dissociating dermal tissue to use in a cell > count on a hemacytometer. Can I vortex cells in suspension or should I just > keep them in the enzyme bath a bit longer? (worried about surface markers > for FACS) > > thanks for any help, > stephen > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Wed Jul 9 06:21:07 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Jul 9 06:21:14 2008 Subject: [Histonet] Single-cell susension from tissue In-Reply-To: <49151.4385.qm@web55606.mail.re4.yahoo.com> References: <1504269054-1215576553-cardhu_decombobulator_blackberry.rim.net-622599549-@bxe157.bisx.prod.on.blackberry> <49151.4385.qm@web55606.mail.re4.yahoo.com> Message-ID: <5b6eb13e0807090421w4f18525bl2e17c6a231db4c71@mail.gmail.com> That's what I've seen most people do when they re-clump. If you see debris everywhere on the side scatter vs forward scatter histogram, you might add some extra washes or digest longer to clear it up too. Cytometers are made for analyzing blood/BM and if there are clumps you can clog it up. Careful! On 7/8/08, Stephen Hyter wrote: > thanks for the replies > > after much trial and error I am using a mixture of Collagenase IV, Dispase > II and a touch of trypsin. It has actually worked pretty good, I should of > mentioned I passed through a 70 micron filter but you could visualize clumps > of cells in the suspension cter on > > I am thinking I will try pipetting them a couple of times before I pass > through the filter, that should help right? > > stephen > > > --- On Tue, 7/8/08, anh2006@med.cornell.edu > wrote: > > > From: anh2006@med.cornell.edu > > Subject: Re: [Histonet] Single-cell susension from tissue > > To: "Mark Tarango" , > histonet-bounces@lists.utsouthwestern.edu, hyters@onid.orst.edu > > Cc: histonet@lists.utsouthwestern.edu > > Date: Tuesday, July 8, 2008, 9:06 PM > > What enzymes are you using for digestion? Just curious .... > > Have you looked into the BD Medimachines for cell > > dissociation? They work great .... So easy! > > > > -----Original Message----- > > From: Mark Tarango > > > > Date: Tue, 08 Jul 2008 20:43:24 > > To: > > Cc: > > Subject: Re: [Histonet] Single-cell susension from tissue > > > > > > Hi Stephen > > > > You can vortex but I'd keep them in the enzyme bath > > longer. If you're > > worried about losing a population of cells (or the epitopes > > getting chewed > > up from digestion), remove the cells that have come out of > > the tissue and > > hold them in PBS. You can put the remaining tissue back > > for digestion (you > > don't want to exclude a population of cells because > > they never came out of > > the tissue). Different populations of cells can come out > > at different times > > and you can even end up selecting out populations based on > > how fast you are > > centrifuging. > > > > Mark > > > > On Tue, Jul 8, 2008 at 7:50 PM, > > wrote: > > > > > hello, having trouble fully dissociating dermal tissue > > to use in a cell > > > count on a hemacytometer. Can I vortex cells in > > suspension or should I just > > > keep them in the enzyme bath a bit longer? (worried > > about surface markers > > > for FACS) > > > > > > thanks for any help, > > > stephen > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > From cory.collins <@t> DHAT.com Wed Jul 9 08:22:02 2008 From: cory.collins <@t> DHAT.com (Cory Collins) Date: Wed Jul 9 08:22:07 2008 Subject: [Histonet] Career opportunities and tissue request Message-ID: Hi Histonet Land, My name is Cory Collins and I'm the supervisor of a new private lab that is weeks away from opening in Dallas, TX. We will be performing pathology services for and as a part of Digestive Health Associates of Texas (DHAT), a group of 70+ GI physicians in the DFW area. The group comprises multiple offices and 5 endoscopy centers. DHAT just finished construction on our brand new, beautiful laboratory stocked with new equipment. We have multiple opening for histotechs, lab aides and billing staff. All shifts will be during daytime working hours. Please email your resume with a cover letter to HR@dhat.com for immediate consideration for these exciting positions with one of the largest physician groups in North Texas. We are also in the process of validating our equipment and procedures and are in need of extra tissue. Please contact me directly at cory.collins@DHAT.com if you are in the DFW area and have access to extra tissue that you can donate. Thanks and I hope to hear from you soon, Cory Cory Collins, HT (ASCP) QIHC Histology Lab Supervisor Digestive Health Associates of Texas 7920 Elmbrook Dr, Suite 104 Dallas, TX 75247 P: (214)442-5807 F: (214)442-5806 www.DHAT.com From cmiller <@t> physlab.com Wed Jul 9 08:33:46 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Wed Jul 9 08:34:52 2008 Subject: [Histonet] Fungus control tissue Message-ID: <001b01c8e1c8$6a5d3020$3d02a8c0@plab.local> I am searching for a good fungus/ GMS control tissue. I can purchase it from Newcomer Supply but if I can trade someone a fungus control block for plenty of AFB control slides purchased from Newcomer I would prefer. I am running low on my own fungus tissue source (which I prefer over purchased). Let me know, thanks Cheri Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From Jim.Chalmers <@t> leica-microsystems.com Wed Jul 9 08:43:24 2008 From: Jim.Chalmers <@t> leica-microsystems.com (Jim.Chalmers@leica-microsystems.com) Date: Wed Jul 9 08:43:34 2008 Subject: [Histonet] Fungus control tissue In-Reply-To: <001b01c8e1c8$6a5d3020$3d02a8c0@plab.local> Message-ID: Hello Cheryl, Having worked in a microbiology lab, what our histology folks did was to take some ground beef and mix it with stock control from the microbiology lab (yeast and/or fungus) then proceed with processing (did the same with AFB). James Chalmers, MT (HHS); MLT (ASCP) "Cheri Miller" To Sent by: histonet-bounces@ cc lists.utsouthwest Subject [Histonet] Fungus control tissue 07/09/2008 08:33 AM I am searching for a good fungus/ GMS control tissue. I can purchase it from Newcomer Supply but if I can trade someone a fungus control block for plenty of AFB control slides purchased from Newcomer I would prefer. I am running low on my own fungus tissue source (which I prefer over purchased). Let me know, thanks Cheri Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From cmiller <@t> physlab.com Wed Jul 9 08:55:52 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Wed Jul 9 08:56:56 2008 Subject: [Histonet] H&E QA , again Message-ID: <002001c8e1cb$80b14110$3d02a8c0@plab.local> Not meaning to flame anyone here but, My daily goal, so to speak is too always hand to the pathologists a slide of trays that are as close to perfection as possible. I never expect "rejection" of our slides. When this happens I investigate, find the cause and then address it. Otherwise the "they are just Histotechs' you know, anyone can do what they do" attitude would be just. It is a matter of pride for me. When I do have issues with difficult cases such as difficulty in cutting I will go to the reading Pathologist and give him a heads up, letting them know the slides are less than optimal. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From anh2006 <@t> med.cornell.edu Wed Jul 9 08:56:31 2008 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Wed Jul 9 08:59:17 2008 Subject: [Histonet] Single-cell susension from tissue In-Reply-To: <5b6eb13e0807090421w4f18525bl2e17c6a231db4c71@mail.gmail.com> References: <1504269054-1215576553-cardhu_decombobulator_blackberry.rim.net-622599549-@bxe157.bisx.prod.on.blackberry><49151.4385.qm@web55606.mail.re4.yahoo.com><5b6eb13e0807090421w4f18525bl2e17c6a231db4c71@mail.gmail.com> Message-ID: <318909138-1215611946-cardhu_decombobulator_blackberry.rim.net-1337730106-@bxe157.bisx.prod.on.blackberry> What if you added some BSA, or maybe some DNase, or something to the solution to prevent clumping. The trypsin worried me for flow antigens as I know it cleaves VEGF receptors. -----Original Message----- From: Mark Tarango Date: Wed, 09 Jul 2008 04:21:07 To: Cc: ; ; ; Subject: Re: [Histonet] Single-cell susension from tissue That's what I've seen most people do when they re-clump. If you see debris everywhere on the side scatter vs forward scatter histogram, you might add some extra washes or digest longer to clear it up too. Cytometers are made for analyzing blood/BM and if there are clumps you can clog it up. Careful! On 7/8/08, Stephen Hyter wrote: > thanks for the replies > > after much trial and error I am using a mixture of Collagenase IV, Dispase > II and a touch of trypsin. It has actually worked pretty good, I should of > mentioned I passed through a 70 micron filter but you could visualize clumps > of cells in the suspension cter on > > I am thinking I will try pipetting them a couple of times before I pass > through the filter, that should help right? > > stephen > > > --- On Tue, 7/8/08, anh2006@med.cornell.edu > wrote: > > > From: anh2006@med.cornell.edu > > Subject: Re: [Histonet] Single-cell susension from tissue > > To: "Mark Tarango" , > histonet-bounces@lists.utsouthwestern.edu, hyters@onid.orst.edu > > Cc: histonet@lists.utsouthwestern.edu > > Date: Tuesday, July 8, 2008, 9:06 PM > > What enzymes are you using for digestion? Just curious .... > > Have you looked into the BD Medimachines for cell > > dissociation? They work great .... So easy! > > > > -----Original Message----- > > From: Mark Tarango > > > > Date: Tue, 08 Jul 2008 20:43:24 > > To: > > Cc: > > Subject: Re: [Histonet] Single-cell susension from tissue > > > > > > Hi Stephen > > > > You can vortex but I'd keep them in the enzyme bath > > longer. If you're > > worried about losing a population of cells (or the epitopes > > getting chewed > > up from digestion), remove the cells that have come out of > > the tissue and > > hold them in PBS. You can put the remaining tissue back > > for digestion (you > > don't want to exclude a population of cells because > > they never came out of > > the tissue). Different populations of cells can come out > > at different times > > and you can even end up selecting out populations based on > > how fast you are > > centrifuging. > > > > Mark > > > > On Tue, Jul 8, 2008 at 7:50 PM, > > wrote: > > > > > hello, having trouble fully dissociating dermal tissue > > to use in a cell > > > count on a hemacytometer. Can I vortex cells in > > suspension or should I just > > > keep them in the enzyme bath a bit longer? (worried > > about surface markers > > > for FACS) > > > > > > thanks for any help, > > > stephen > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > From settembr <@t> umdnj.edu Wed Jul 9 09:59:44 2008 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Wed Jul 9 10:00:20 2008 Subject: [Histonet] VZV antibody Message-ID: Hello Erin, I use Novocastra's VZV on FFPE human tissue. I do not pretreat; I use the antibody at 1:75 I use Dako's LSAB2 detection kit. I hope this helps. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> "Martin, Erin" 07/08/08 1:04 PM >>> Hi everyone, I am having a really hard time getting the Varicella Zoster Virus antibody from Novocastra to work. If anyone is currently using this, would you mind sharing your procedure? Thanks, Erin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ploykasek <@t> phenopath.com Wed Jul 9 10:06:06 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Wed Jul 9 10:06:22 2008 Subject: [Histonet] H&E QA , again In-Reply-To: <002001c8e1cb$80b14110$3d02a8c0@plab.local> Message-ID: Always a good goal, Cheryl. I work in a reference lab so we have 'tissue issues' with outside blocks that are out of our control. One way we cope with 'tissue issues' beyond our control is to have a form that the histotechs fill out regarding bad processing, embedding, ect... This form goes with the case to the doc, and documents these issues. This way the doc knows we have had a problem (& aren't just slacking!) , he can request reprocessing or re-embedding if desired. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Not meaning to flame anyone here but, My daily goal, so to speak is too > always hand to the pathologists a slide of trays that are as close to > perfection as possible. I never expect "rejection" of our slides. When this > happens I investigate, find the cause and then address it. Otherwise the > "they are just Histotechs' you know, anyone can do what they do" attitude > would be just. It is a matter of pride for me. When I do have issues with > difficult cases such as difficulty in cutting I will go to the > > reading Pathologist and give him a heads up, letting them know the slides > are less than optimal. > > > > Cheryl Miller HT (ASCP) > > Histology Supervisor > > Physicians Laboratory,P.C. > > Omaha, Ne. > > 402 738 5052 > > > > > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If > you are not the addressee intended / indicated or agent responsible for > delivering it to the addressee, you are hereby notified that you are in > possession of confidential and privileged information. Any dissemination, > distribution, or copying of this e-mail is strictly prohibited. If you have > received this message in error, please notify the sender immediately and > delete this email from your system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From schaundrawalton <@t> yahoo.com Wed Jul 9 10:27:19 2008 From: schaundrawalton <@t> yahoo.com (Schaundra Walton) Date: Wed Jul 9 10:27:23 2008 Subject: [Histonet] Slide Warmers and Flotation Baths Message-ID: <401097.43829.qm@web58909.mail.re1.yahoo.com> Is anybody else having trouble getting slide warmers or flotation baths?? I'm having the most trouble with the flotation baths.? I'd like to replace one that is going bad with something comparable.? I am currently using a rectangular bath with a glass pyrex dish.? I've been told that this type of water bath is no longer being produced and the only ones available are the round ones.? Has anyoen else heard of this?? Does anyone know of a manufacturer or distributer that might have?rectangular ones?readily available?? Any help would be greatly appreciated. ? Thanks, Schaundra Walton BS, HTL(ASCP) Histology Supervisor Swedish American Hospital Rockford, IL From kgrobert <@t> rci.rutgers.edu Wed Jul 9 10:57:19 2008 From: kgrobert <@t> rci.rutgers.edu (Kathleen Roberts) Date: Wed Jul 9 10:47:11 2008 Subject: [Histonet] Slide Warmers and Flotation Baths In-Reply-To: <401097.43829.qm@web58909.mail.re1.yahoo.com> References: <401097.43829.qm@web58909.mail.re1.yahoo.com> Message-ID: <4874DFDF.8030805@rci.rutgers.edu> Fisher Scientific has one just like the one you described: http://www.fishersci.com/wps/portal/PRODUCTDETAIL?productId=4753211&catalogId=29104&pos=2&catCode=RE_SC&fromCat=yes&keepSessionSearchOutPut=true&brCategoryId=55899&hlpi=y&fromSearch= Kathy Principal Lab Technician Neurotoxicology Labs Rutgers University, Piscataway, NJ Schaundra Walton wrote: >Is anybody else having trouble getting slide warmers or flotation baths? I'm having the most trouble with the flotation baths. I'd like to replace one that is going bad with something comparable. I am currently using a rectangular bath with a glass pyrex dish. I've been told that this type of water bath is no longer being produced and the only ones available are the round ones. Has anyoen else heard of this? Does anyone know of a manufacturer or distributer that might have rectangular ones readily available? Any help would be greatly appreciated. > >Thanks, >Schaundra Walton BS, HTL(ASCP) >Histology Supervisor >Swedish American Hospital >Rockford, IL > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From mbisher <@t> Princeton.EDU Wed Jul 9 10:58:24 2008 From: mbisher <@t> Princeton.EDU (Peggy Bisher) Date: Wed Jul 9 10:58:33 2008 Subject: [Histonet] Slide Warmers and Flotation Baths In-Reply-To: <401097.43829.qm@web58909.mail.re1.yahoo.com> Message-ID: I just purchased these two items in September 2007. Both were ordered from Fisher Scientific. The Tissue bath was Cat #22 265 176 And the Slide warmer was Cat#11 695 5Q Both had LED displays for the temperature. I am very pleased with both of them. Good Luck, Peggy Margaret E. Bisher Electron Microscopy & Histology Core Facility Manager Department of Molecular Biology Princeton University Moffett Laboratory, Room 113 Princeton, New Jersey Office: (609) 258-7026 Fax: (609) 258-8468 mbisher@princeton.edu On 7/9/08 11:27 AM, "Schaundra Walton" wrote: > Is anybody else having trouble getting slide warmers or flotation baths?? I'm > having the most trouble with the flotation baths.? I'd like to replace one > that is going bad with something comparable.? I am currently using a > rectangular bath with a glass pyrex dish.? I've been told that this type of > water bath is no longer being produced and the only ones available are the > round ones.? Has anyoen else heard of this?? Does anyone know of a > manufacturer or distributer that might have?rectangular ones?readily > available?? Any help would be greatly appreciated. > ? > Thanks, > Schaundra Walton BS, HTL(ASCP) > Histology Supervisor > Swedish American Hospital > Rockford, IL > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gentras <@t> vetmed.auburn.edu Wed Jul 9 10:59:40 2008 From: gentras <@t> vetmed.auburn.edu (Atoska Gentry) Date: Wed Jul 9 10:59:44 2008 Subject: [Histonet] RE: Tissue Processors Message-ID: <4874E06C.7040601@vetmed.auburn.edu> Hello, thanks to all who responded to my initial inquiry. Your assistance has aided in my ability to narrow my search. It has been brought to my attention that any processor other than Microm's STP120 Spin Tissue Processor and/or ThermoShandon's discontinued TPC12 would be overkill for both our processing volume and budget. Therefore, I would be most appreciative if those of you using either of these processors or something similar will share with me the pros/cons , likes /dislikes ASAP. Thank you kindly, Atoska From mbisher <@t> Princeton.EDU Wed Jul 9 11:21:22 2008 From: mbisher <@t> Princeton.EDU (Peggy Bisher) Date: Wed Jul 9 11:22:37 2008 Subject: [Histonet] RE: Tissue Processors In-Reply-To: <4874E06C.7040601@vetmed.auburn.edu> Message-ID: I deal with a company called Electron Microscopy Sciences for a lot of my electron microscopy consumables. A portion of their business is also with Histology products. They do sell a small (desktop) automated tissue processor. They actually have two models, the LYNX II and the LYNX el. Both are probably a lot cheaper then a stand alone system that you mentioned. Give them a call, they have a great website, perhaps you might like what they offer instead. I myself have not used these processors, but I have been a customer of theirs for many years and I very happy with them. Good Luck, Peggy Margaret E. Bisher Electron Microscopy & Histology Core Facility Manager Department of Molecular Biology Princeton University Moffett Laboratory, Room 113 Princeton, New Jersey Office: (609) 258-7026 Fax: (609) 258-8468 mbisher@princeton.edu On 7/9/08 11:59 AM, "Atoska Gentry" wrote: > Hello, thanks to all who responded to my initial inquiry. Your > assistance has aided in my ability to narrow my search. It has been > brought to my attention that any processor other than Microm's STP120 > Spin Tissue Processor and/or ThermoShandon's discontinued TPC12 would > be overkill for both our processing volume and budget. Therefore, I > would be most appreciative if those of you using either of these > processors or something similar will share with me the pros/cons , > likes /dislikes ASAP. Thank you kindly, Atoska > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Wed Jul 9 12:23:48 2008 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Jul 9 12:23:58 2008 Subject: [Histonet] Slide Warmers and Flotation Baths In-Reply-To: <401097.43829.qm@web58909.mail.re1.yahoo.com> Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F593@lmhsmail.lmhealth.org> I just got a rectangular one from Cardinal Health Systems (Alliegance). Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Schaundra Walton Sent: Wednesday, July 09, 2008 11:27 AM To: Histonet Subject: [Histonet] Slide Warmers and Flotation Baths Is anybody else having trouble getting slide warmers or flotation baths?? I'm having the most trouble with the flotation baths.? I'd like to replace one that is going bad with something comparable.? I am currently using a rectangular bath with a glass pyrex dish.? I've been told that this type of water bath is no longer being produced and the only ones available are the round ones.? Has anyoen else heard of this?? Does anyone know of a manufacturer or distributer that might have?rectangular ones?readily available?? Any help would be greatly appreciated. ? Thanks, Schaundra Walton BS, HTL(ASCP) Histology Supervisor Swedish American Hospital Rockford, IL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Wed Jul 9 12:38:04 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Jul 9 12:31:09 2008 Subject: [Histonet] RE: Tissue Processors In-Reply-To: <4874E06C.7040601@vetmed.auburn.edu> References: <4874E06C.7040601@vetmed.auburn.edu> Message-ID: <5A2BD13465E061429D6455C8D6B40E390B77E24C@IBMB7Exchange.digestivespecialists.com> Atoska, I used the STP 120 before we out grew it and liked it. Just keep extra fuses on hand for the paraffin pots. That was my only problem. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Atoska Gentry Sent: Wednesday, July 09, 2008 12:00 PM To: Histonet Subject: [Histonet] RE: Tissue Processors Hello, thanks to all who responded to my initial inquiry. Your assistance has aided in my ability to narrow my search. It has been brought to my attention that any processor other than Microm's STP120 Spin Tissue Processor and/or ThermoShandon's discontinued TPC12 would be overkill for both our processing volume and budget. Therefore, I would be most appreciative if those of you using either of these processors or something similar will share with me the pros/cons , likes /dislikes ASAP. Thank you kindly, Atoska _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bauer.Karen <@t> mayo.edu Wed Jul 9 12:57:40 2008 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen) Date: Wed Jul 9 12:57:51 2008 Subject: [Histonet] Tissue Tek Xpress Message-ID: If you are using a Tissue Tek Xpress or Xpress 50, could you please contact me by email? I would like to ask you a few questions. Thanks in advance, Karen Karen L. Bauer HT(ASCP) Department of Pathology Histology Section Chief Luther Hospital Eau Claire, WI 715-838-3205 ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. From PMonfils <@t> Lifespan.org Wed Jul 9 14:05:42 2008 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Jul 9 14:05:50 2008 Subject: [Histonet] Slide Warmers and Flotation Baths In-Reply-To: <44846.95298.qm@web58911.mail.re1.yahoo.com> Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835C2B@LSRIEXCH1.lsmaster.lifespan.org> Try www.mopec.com From gentras <@t> vetmed.auburn.edu Wed Jul 9 14:18:30 2008 From: gentras <@t> vetmed.auburn.edu (Atoska Gentry) Date: Wed Jul 9 14:18:35 2008 Subject: [Histonet] RE: Tissue Processors correction Message-ID: <48750F06.8080103@vetmed.auburn.edu> Hello, thanks to all who responded to my initial inquiry. Your assistance has aided in my ability to narrow my search. It has been brought to my attention that any processor other than: Microm's STP120 Spin Tissue Processor, ThermoShandon's Citadel 2000 or Medite's discontinued TPC12 and/or Leica's TP 1020 would be overkill for both our processing volume and budget. Therefore, I would be most appreciative if those of you using either of these carousel-type tissue processors or something similar will share with me the pros/cons,likes /dislikes ASAP. Thank you kindly, Atoska _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgalnow <@t> aol.com Wed Jul 9 14:22:34 2008 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Wed Jul 9 14:22:57 2008 Subject: [Histonet] Imaging systems for IHC and FISH Message-ID: <8CAB0104683241F-1144-2612@FWM-D12.sysops.aol.com> Hello everyone, I first want to thank you for all of the questions you have helped myself an others with throughout the years.? That being said, here is another one....... Has anyone used or considered using the ARIOL system from Applied Imaging?? If you have what are your likes and dislikes of the instrument, if you have not, then why did you decide against it? If you had to do it all over again, would you purchase it?? Why/why not? Again, I thank you for your time. Roxanne From mickie25 <@t> netzero.net Wed Jul 9 14:28:10 2008 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Wed Jul 9 14:27:54 2008 Subject: [Histonet] Slide Warmers and Flotation Baths In-Reply-To: <401097.43829.qm@web58909.mail.re1.yahoo.com> References: <401097.43829.qm@web58909.mail.re1.yahoo.com> Message-ID: Dear Shaundra, This water bath is still being made and sold by Belair Instrument Company in Springfield, NJ. Their telephone number is: 800-783-9424. They will be happy to help you out. Best Regards, Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Schaundra Walton Sent: Wednesday, July 09, 2008 8:27 AM To: Histonet Subject: [Histonet] Slide Warmers and Flotation Baths Is anybody else having trouble getting slide warmers or flotation baths?? I'm having the most trouble with the flotation baths.? I'd like to replace one that is going bad with something comparable.? I am currently using a rectangular bath with a glass pyrex dish.? I've been told that this type of water bath is no longer being produced and the only ones available are the round ones.? Has anyoen else heard of this?? Does anyone know of a manufacturer or distributer that might have?rectangular ones?readily available?? Any help would be greatly appreciated. ? Thanks, Schaundra Walton BS, HTL(ASCP) Histology Supervisor Swedish American Hospital Rockford, IL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Internal Virus Database is out of date. Checked by AVG - http://www.avg.com Version: 8.0.134 / Virus Database: 270.4.5/1533 - Release Date: 7/3/2008 7:19 PM From gentras <@t> vetmed.auburn.edu Wed Jul 9 14:29:37 2008 From: gentras <@t> vetmed.auburn.edu (Atoska Gentry) Date: Wed Jul 9 14:29:41 2008 Subject: [Histonet] Microm STP 120 Spin Tissue Processor Message-ID: <487511A1.9060705@vetmed.auburn.edu> Will someone with detailed info on the Microm STP 120 Spin Tissue Processor please contact me ASAP? Thanks, Atoska From donna_suresch <@t> merck.com Wed Jul 9 16:03:40 2008 From: donna_suresch <@t> merck.com (Suresch, Donna L.) Date: Wed Jul 9 16:03:46 2008 Subject: [Histonet] Staining Artifacts with Tape Transfer Message-ID: If anyone has any suggestions on removing staining artifacts from the slide adhesive on tape transfer frozen sections, it would be appreciated. I am staining vessels with H&E and Oil Red O and have tried slides with 0.5x, 1x, and 4x adhesive. Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From Carol.Fields <@t> Northside.com Wed Jul 9 16:14:52 2008 From: Carol.Fields <@t> Northside.com (Carol Fields) Date: Wed Jul 9 16:15:00 2008 Subject: [Histonet] Histo tech, ASCP- position available Message-ID: <038809F21D1F9040A1FEAD72A7E2C6B509724658@NSMXMS04.northside.local> Northside Hospital a busy 444 bed, not for profit hospital in the popular Sandy Springs area of Atlanta, has an immediate opening for a Histotechnologist, ASCP. Renowned for its expertise in women's services, Northside ranks first in the nation among community hospitals in the number of babies delivered. Northside is accredited by Joint Commission, (JCAHO), CAP, and named as Atlanta's most preferred hospital for over all health care services in the National Healthcare Market Guide Survey. Northside offers great benefits, competitive salaries, and state of the art equipment. Call Royce Roberts, Human Resources at 404-851-8748 for further information. Send resume to royce.roberts@northside.com and carol.fields@northside.com. CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. From ruebenjcarter <@t> gmail.com Wed Jul 9 16:35:09 2008 From: ruebenjcarter <@t> gmail.com (R C) Date: Wed Jul 9 16:35:15 2008 Subject: [Histonet] Shandon vs Sakura block/slide labelers Message-ID: <2a926e3f0807091435y7df33e3al4be3be07788166ff@mail.gmail.com> We're entertaining two products. The Sakura Tissue Tek Auto Write Printer and the Thermo Shandon Microwriter I Cassette labeler. Has anyone had any first hand experience with either brands? A slide labeler is projected in the future as well. Thanks. Rueben Carter From jstn192 <@t> yahoo.com Wed Jul 9 16:59:09 2008 From: jstn192 <@t> yahoo.com (Justin Thomas) Date: Wed Jul 9 16:59:19 2008 Subject: [Histonet] SUPPLY AND CHEMICAL COMPANY Message-ID: <634317.30982.qm@web35707.mail.mud.yahoo.com> Hello all: ? We are a Fisher distributor for all laboratory suppliers.? Also, we supply laboratory chemicals of high quality and a more economical rate.? ? We have a our machine and fabrication business that strictly manufacturers items for histology and pathology.? Has a part to a piece of equipment broken?? And you have received a quote from the OEM and it?s EXPENSIVE?!? We can manufacture that same part at our facility for a fraction of the OEM price. ? PLEASE give us a call for quotes: ? CMD, LLC. 803-633-2124 ? Office 803-635-9401 ? Fax ? Ask for anyone is sales! ? From jnocito <@t> satx.rr.com Wed Jul 9 17:57:28 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Jul 9 17:57:00 2008 Subject: [Histonet] H&E QA , again References: Message-ID: <004c01c8e217$2984bfb0$0302a8c0@yourxhtr8hvc4p> at my last place of employment, we were a referral lab for a hospital. Standard protocol was to reprocess all the blocks and cut an H&E before sending the case to the pathologist. This hospital had the worst histology that I've even seen. I would be embarrassed to put out such substandard work. I volunteered my services to maybe assist with issues, but was turned down flat. I do not know how the pathologist made an accurate diagnosis or how they didn't get creamed by CAP. JTT ----- Original Message ----- From: "Patti Loykasek" To: "Cheri Miller" ; "histonet" Sent: Wednesday, July 09, 2008 10:06 AM Subject: Re: [Histonet] H&E QA , again Always a good goal, Cheryl. I work in a reference lab so we have 'tissue issues' with outside blocks that are out of our control. One way we cope with 'tissue issues' beyond our control is to have a form that the histotechs fill out regarding bad processing, embedding, ect... This form goes with the case to the doc, and documents these issues. This way the doc knows we have had a problem (& aren't just slacking!) , he can request reprocessing or re-embedding if desired. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Not meaning to flame anyone here but, My daily goal, so to speak is too > always hand to the pathologists a slide of trays that are as close to > perfection as possible. I never expect "rejection" of our slides. When > this > happens I investigate, find the cause and then address it. Otherwise the > "they are just Histotechs' you know, anyone can do what they do" attitude > would be just. It is a matter of pride for me. When I do have issues with > difficult cases such as difficulty in cutting I will go to the > > reading Pathologist and give him a heads up, letting them know the slides > are less than optimal. > > > > Cheryl Miller HT (ASCP) > > Histology Supervisor > > Physicians Laboratory,P.C. > > Omaha, Ne. > > 402 738 5052 > > > > > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. > If > you are not the addressee intended / indicated or agent responsible for > delivering it to the addressee, you are hereby notified that you are in > possession of confidential and privileged information. Any dissemination, > distribution, or copying of this e-mail is strictly prohibited. If you > have > received this message in error, please notify the sender immediately and > delete this email from your system. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Linda.Watson <@t> bms.com Thu Jul 10 05:20:05 2008 From: Linda.Watson <@t> bms.com (Linda M Watson) Date: Thu Jul 10 05:20:12 2008 Subject: [Histonet] Imaging systems for IHC and FISH In-Reply-To: <8CAB0104683241F-1144-2612@FWM-D12.sysops.aol.com> References: <8CAB0104683241F-1144-2612@FWM-D12.sysops.aol.com> Message-ID: <4875E255.3080102@bms.com> Hi Roxanne, We purchased the Ariol SL-50 4 years ago. In the past we used Image Pro Plus for most all our image analysis, if applicable. It served it purpose but was extremely time consuming. The Ariol SL-50 changed our lives for the better. Analysis using IPP would take up to a week for two to complete. Those same experiments would be done in 2 days. The company, Applied Imaging-now Genetix has a very good product manager and has taken many suggestions from us and others to upgrade the software to meet our needs. Keep in mind that, as many of us know, computer driven instruments can sometimes have littel hic-ups but their tech services are always available to help get you back on line. Overall, I love our instrument. I would suggest that you look at other companies and make comparisons. The Ariol does everything that we need it to do but it may not meet all your needs. Linda godsgalnow@aol.com wrote: >Hello everyone, > >I first want to thank you for all of the questions you have helped myself an others with throughout the years.? That being said, here is another one....... > >Has anyone used or considered using the ARIOL system from Applied Imaging?? If you have what are your likes and dislikes of the instrument, if you have not, then why did you decide against it? > >If you had to do it all over again, would you purchase it?? Why/why not? > >Again, I thank you for your time. > >Roxanne >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From carrolpb <@t> umdnj.edu Thu Jul 10 08:13:29 2008 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Thu Jul 10 08:13:54 2008 Subject: [Histonet] SUPPLY AND CHEMICAL COMPANY In-Reply-To: <634317.30982.qm@web35707.mail.mud.yahoo.com> References: <634317.30982.qm@web35707.mail.mud.yahoo.com> Message-ID: <48760AF9.7060802@umdnj.edu> Is unsolicited spam like this generally allowed on this list? Justin Thomas wrote: > Hello all: > > We are a Fisher distributor for all laboratory suppliers. Also, we supply laboratory chemicals of high quality and a more economical rate. > > We have a our machine and fabrication business that strictly manufacturers items for histology and pathology. Has a part to a piece of equipment broken? And you have received a quote from the OEM and it?s EXPENSIVE?! We can manufacture that same part at our facility for a fraction of the OEM price. > > PLEASE give us a call for quotes: > > CMD, LLC. > 803-633-2124 ? Office > 803-635-9401 ? Fax > > Ask for anyone is sales! > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From mpence <@t> grhs.net Thu Jul 10 08:20:36 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Jul 10 08:20:49 2008 Subject: [Histonet] SUPPLY AND CHEMICAL COMPANY In-Reply-To: <48760AF9.7060802@umdnj.edu> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A388E@IS-E2K3.grhs.net> I do not find this "spam" to be as annoying as when I get a unsolicited e-mail from someone I have never heard of that hits my inbox and it is not from the listserv, but you know they have gotten your e-mail from the listserv. I found this to be a piece of information I will put away for future use. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Peter Carroll Sent: Thursday, July 10, 2008 8:13 AM To: jstn192@yahoo.com Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] SUPPLY AND CHEMICAL COMPANY Is unsolicited spam like this generally allowed on this list? Justin Thomas wrote: > Hello all: > > We are a Fisher distributor for all laboratory suppliers. Also, we > supply laboratory chemicals of high quality and a more economical rate. > > We have a our machine and fabrication business that strictly > manufacturers items for histology and pathology. Has a part to a piece of equipment broken? And you have received a quote from the OEM and it's EXPENSIVE?! We can manufacture that same part at our facility for a fraction of the OEM price. > > PLEASE give us a call for quotes: > > CMD, LLC. > 803-633-2124 - Office > 803-635-9401 - Fax > > Ask for anyone is sales! > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ROrr <@t> enh.org Thu Jul 10 08:42:17 2008 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Thu Jul 10 08:42:23 2008 Subject: [Histonet] VZV Message-ID: Hi Erin I use the VZV from Novacastra. It works best for me when made up fresh each run. If you have run the antibody fresh, then try using fresh antibody and cut "fresh" sections from the paraffin block for control tissue. I run it 1:50 with Biocare Mach 3. If you are using and ABC kit of anykind you may need to go even stronger, 1:10. Try one slide with pepsin and one with out pepsin. We get best results with no HIER or enzyme pretreatment. Hope this helps. Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 From rjbuesa <@t> yahoo.com Thu Jul 10 09:13:31 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jul 10 09:13:35 2008 Subject: [Histonet] SUPPLY AND CHEMICAL COMPANY In-Reply-To: <48760AF9.7060802@umdnj.edu> Message-ID: <313340.35091.qm@web65702.mail.ac4.yahoo.com> No, but from time to time somebody "sneaks in", the economy is rough, you know! Ren? J. --- On Thu, 7/10/08, Peter Carroll wrote: From: Peter Carroll Subject: Re: [Histonet] SUPPLY AND CHEMICAL COMPANY To: jstn192@yahoo.com Cc: histonet@lists.utsouthwestern.edu Date: Thursday, July 10, 2008, 9:13 AM Is unsolicited spam like this generally allowed on this list? From micropathlabs <@t> yahoo.com Thu Jul 10 09:50:37 2008 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Thu Jul 10 09:50:45 2008 Subject: [Histonet] Frozen Section controls Message-ID: <904128.32580.qm@web57812.mail.re3.yahoo.com> Here's a new one on me. At recent inspection I was told we should be running a frozen section control slide?with our metachromatic stain daily.?The inspector was at an off-site facility where we do not have capability of keeping tissue frozen from one day to next not to mention that the metachromatic stain is not permanent (he told?me we had to?hold slides for 2 years).?I also explained?that our pathologists do indicate acceptability of stain daily?but he?said that was not sufficient?since the pathologist was looking at patient tissue instead of control tissue. When I explained issue of inability?to keep tissue frozen (we have numerous off-site locations) he?said to cut an?initial, superficial slide of patient tissue to use as control then proceed with patient frozen section after approval of stain.?Any one?heard of this??Seems a bit much to?me. ? Sheila Haas Laboratory Supervisor From mpence <@t> grhs.net Thu Jul 10 10:05:54 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Jul 10 10:06:00 2008 Subject: [Histonet] Frozen Section controls In-Reply-To: <904128.32580.qm@web57812.mail.re3.yahoo.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A388F@IS-E2K3.grhs.net> Was this a CAP inspection and was you sited? OR was this a recommendation? Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Thursday, July 10, 2008 9:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen Section controls Here's a new one on me. At recent inspection I was told we should be running a frozen section control slide?with our metachromatic stain daily.?The inspector was at an off-site facility where we do not have capability of keeping tissue frozen from one day to next not to mention that the metachromatic stain is not permanent (he told?me we had to?hold slides for 2 years).?I also explained?that our pathologists do indicate acceptability of stain daily?but he?said that was not sufficient?since the pathologist was looking at patient tissue instead of control tissue. When I explained issue of inability?to keep tissue frozen (we have numerous off-site locations) he?said to cut an?initial, superficial slide of patient tissue to use as control then proceed with patient frozen section after approval of stain.?Any one?heard of this??Seems a bit much to?me. ? Sheila Haas Laboratory Supervisor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From schaundrawalton <@t> yahoo.com Thu Jul 10 11:03:36 2008 From: schaundrawalton <@t> yahoo.com (Schaundra Walton) Date: Thu Jul 10 11:03:40 2008 Subject: [Histonet] Tracking Floaters Message-ID: <736408.84349.qm@web58914.mail.re1.yahoo.com> Hi Netters! ? I'm curious how do other labs track floaters on slides?? I'd like to do QI regarding this topic and I'm curious what other people are doing.? How are you tracking which tech, location of contamination, resolution, etc.? ? Thanks, Schaundra Walton BS, HTL(ASCP) Histology Supervisor Swedish American Hospital Rockford, IL From BFicher <@t> chomp.org Thu Jul 10 11:16:38 2008 From: BFicher <@t> chomp.org (Fischer, R. B) Date: Thu Jul 10 11:16:44 2008 Subject: [Histonet] Tracking Floaters In-Reply-To: <736408.84349.qm@web58914.mail.re1.yahoo.com> References: <736408.84349.qm@web58914.mail.re1.yahoo.com> Message-ID: Shaundra, We track our floaters thru a daily communication log that we send with the slides to our pathologists. This log reviews the quality of the H&E stain,recuts, labeling errors, floaters, quality of special stains, as well as immunos,microtomy, blocks to reprocess, recuts for QA, and any other comments the pathologist may have. This sheet is returned to us the following morning. If there are any floaters noted, the Dr. will return these also for our review. We know who cut the slides since we initial all the slides we cut. At the end of the month many of these subjects are tallied in a quality assurance summary report as well as a performance management report. R.Brian Fischer Histology Lead Tech Community Hospital of the Monterey Peninsula PO Box HH Monterey Ca. 93942 831-625-4791 Fax: 831-6583683 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Schaundra Walton Sent: Thursday, July 10, 2008 9:04 AM To: Histonet Subject: [Histonet] Tracking Floaters Hi Netters! ? I'm curious how do other labs track floaters on slides?? I'd like to do QI regarding this topic and I'm curious what other people are doing.? How are you tracking which tech, location of contamination, resolution, etc.? ? Thanks, Schaundra Walton BS, HTL(ASCP) Histology Supervisor Swedish American Hospital Rockford, IL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. From JWeems <@t> sjha.org Thu Jul 10 11:21:56 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Jul 10 11:22:08 2008 Subject: [Histonet] Tracking Floaters In-Reply-To: References: <736408.84349.qm@web58914.mail.re1.yahoo.com> Message-ID: <982A0A9461F9BF438C7B19A6E425A383373AB6@ITSSSXM01V6.one.ads.che.org> And if you have your techs initial the slides, you can track patterns... j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fischer, R. B Sent: Thursday, July 10, 2008 12:17 PM To: schaundrawalton@yahoo.com Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Tracking Floaters Shaundra, We track our floaters thru a daily communication log that we send with the slides to our pathologists. This log reviews the quality of the H&E stain,recuts, labeling errors, floaters, quality of special stains, as well as immunos,microtomy, blocks to reprocess, recuts for QA, and any other comments the pathologist may have. This sheet is returned to us the following morning. If there are any floaters noted, the Dr. will return these also for our review. We know who cut the slides since we initial all the slides we cut. At the end of the month many of these subjects are tallied in a quality assurance summary report as well as a performance management report. R.Brian Fischer Histology Lead Tech Community Hospital of the Monterey Peninsula PO Box HH Monterey Ca. 93942 831-625-4791 Fax: 831-6583683 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Schaundra Walton Sent: Thursday, July 10, 2008 9:04 AM To: Histonet Subject: [Histonet] Tracking Floaters Hi Netters! ? I'm curious how do other labs track floaters on slides?? I'd like to do QI regarding this topic and I'm curious what other people are doing.? How are you tracking which tech, location of contamination, resolution, etc.? ? Thanks, Schaundra Walton BS, HTL(ASCP) Histology Supervisor Swedish American Hospital Rockford, IL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From rjbuesa <@t> yahoo.com Thu Jul 10 11:32:16 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jul 10 11:32:19 2008 Subject: [Histonet] Frozen Section controls In-Reply-To: <904128.32580.qm@web57812.mail.re3.yahoo.com> Message-ID: <370328.20904.qm@web65705.mail.ac4.yahoo.com> Whenever I got across one inspector demanding unjustified actions, I let the medical director to deal with them, they "talk the same language". Do not lose sleep over this demand, let your pathologist to deal with it. Never heard before. Ren? J. --- On Thu, 7/10/08, Sheila Haas wrote: From: Sheila Haas Subject: [Histonet] Frozen Section controls To: histonet@lists.utsouthwestern.edu Date: Thursday, July 10, 2008, 10:50 AM Here's a new one on me. At recent inspection I was told we should be running a frozen section control slide?with our metachromatic stain daily.?The inspector was at an off-site facility where we do not have capability of keeping tissue frozen from one day to next not to mention that the metachromatic stain is not permanent (he told?me we had to?hold slides for 2 years).?I also explained?that our pathologists do indicate acceptability of stain daily?but he?said that was not sufficient?since the pathologist was looking at patient tissue instead of control tissue. When I explained issue of inability?to keep tissue frozen (we have numerous off-site locations) he?said to cut an?initial, superficial slide of patient tissue to use as control then proceed with patient frozen section after approval of stain.?Any one?heard of this??Seems a bit much to?me. ? Sheila Haas Laboratory Supervisor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kenneth.metzger <@t> aruplab.com Thu Jul 10 11:41:57 2008 From: kenneth.metzger <@t> aruplab.com (Metzger, Kenneth) Date: Thu Jul 10 11:40:32 2008 Subject: [Histonet] Labeling Errors Message-ID: Can anyone tell me there approximate slide labeling errors per 10,000 slides? Thanks, Ken Ken Metzger HTL(ASCP) Histology Supervisor ARUP Laboratories 500 Chipeta way Salt Lake City, UT 84108 801.583.2787 ext 3101 - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From laurie.colbert <@t> huntingtonhospital.com Thu Jul 10 11:42:40 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Thu Jul 10 11:42:44 2008 Subject: [Histonet] Tracking Floaters Message-ID: <57BE698966D5C54EAE8612E8941D7683034B3EA0@EXCHANGE3.huntingtonhospital.com> We have had some issues with floaters, but my problem is that we can't determine whether the floaters were introduced at the time of grossing or embedding. Any suggestions on how to handle the problem, other than constant "reminding?" -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Schaundra Walton Sent: Thursday, July 10, 2008 9:04 AM To: Histonet Subject: [Histonet] Tracking Floaters Hi Netters! ? I'm curious how do other labs track floaters on slides?? I'd like to do QI regarding this topic and I'm curious what other people are doing.? How are you tracking which tech, location of contamination, resolution, etc.? ? Thanks, Schaundra Walton BS, HTL(ASCP) Histology Supervisor Swedish American Hospital Rockford, IL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Robinsoc <@t> mercyhealth.com Thu Jul 10 12:08:58 2008 From: Robinsoc <@t> mercyhealth.com (Cynthia Robinson) Date: Thu Jul 10 12:09:15 2008 Subject: [Histonet] Tracking Floaters In-Reply-To: <57BE698966D5C54EAE8612E8941D7683034B3EA0@EXCHANGE3.huntingtonhospital.com> References: <57BE698966D5C54EAE8612E8941D7683034B3EA0@EXCHANGE3.huntingtonhospital.com> Message-ID: <4875FBDA.59BC.00AF.0@mercyhealth.com> We have been addressing the floater issue. We found that changing to smaller mesh type cassettes has helped but not eliminated the problem even with meticulous care at embedding station (wiping and changing pick ups in between each specimen, pick ups without any ridges, etc). Our paths see tiny, like 10 or less clusters of cells from placenta usually, on some slides but when we cut deeper sections the floaters are gone. Usually it seems to be bone sections which will 'hold onto' these tiny groups of cells. We are continuing to try to fix this issue and would like to hear other suggestions. Thanks. Cindi Robinson HT(ASCP) MMC-Sioux City Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 >>> "Laurie Colbert" 7/10/2008 11:42 AM >>> We have had some issues with floaters, but my problem is that we can't determine whether the floaters were introduced at the time of grossing or embedding. Any suggestions on how to handle the problem, other than constant "reminding?" -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Schaundra Walton Sent: Thursday, July 10, 2008 9:04 AM To: Histonet Subject: [Histonet] Tracking Floaters Hi Netters! I'm curious how do other labs track floaters on slides? I'd like to do QI regarding this topic and I'm curious what other people are doing. How are you tracking which tech, location of contamination, resolution, etc.? Thanks, Schaundra Walton BS, HTL(ASCP) Histology Supervisor Swedish American Hospital Rockford, IL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sally.schlessinger <@t> abbott.com Thu Jul 10 12:32:09 2008 From: sally.schlessinger <@t> abbott.com (Sally H Schlessinger) Date: Thu Jul 10 12:30:43 2008 Subject: [Histonet] Beecher ATA-27 TMA Message-ID: Does anyone use the automated Tissue Micro-arrayer, ATA-27, from Beecher? I'm new to it and would like some advice from a seasoned user. Thanks, Sally Schlessinger Abbott Laboratories From Jackie.O'Connor <@t> abbott.com Thu Jul 10 12:35:41 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Jul 10 12:35:59 2008 Subject: [Histonet] SOS! SOS! Beecher ATA27 Automatic Tissue Arrayer In-Reply-To: <4873A68C.7000108@vetmed.auburn.edu> Message-ID: I have a friend in another lab who is urgent need of help with a tissue arrayer that seems to be stuck in one position and she is at risk of losing her array. Calls to Beecher are going unanswered - can anyone help? contact me and I will forward your information to them! Thanks! Jackie O' From pinetree110567 <@t> yahoo.com Thu Jul 10 13:18:26 2008 From: pinetree110567 <@t> yahoo.com (Jack Cates) Date: Thu Jul 10 13:18:38 2008 Subject: [Histonet] RE: Shandon vs Sakura block/slide labelers Message-ID: <504844.75848.qm@web44906.mail.sp1.yahoo.com> Ruben, I have used both in the past at two different employers.? I have definitely found that the Shandon (RA Lamb, www.ralamb.com?or TBS, www.trianglebiomedical.com ) writer to be far superior.? The Shandon print medium/tape is impervious to all reagents and does not require any curing time to "set" and the footprint is much, much smaller.? Also the cost of operation is not even close... the tape for the Shandon unit sells for approximately $25 dollars and you can get up to 20,000 cassettes, but the Sakura you will need to purchase ink and replace print nozzles, which can be very costly.? I think we found that for the same amount of prints/cassettes the price difference could be up to $1500 dollars/year.... pretty substantial.? The way that I have come to to think about this... its sort of similar to the situation that when you purchase your home PC, they almost invariably give you a printer for free and make their money back on the sale of ink and or print heads.? Also, with the Sandon unit you will have many more outlets for peripherals, like Foil, cassettes and more.? (I have seen PSL, TBS, RA Lamb, ThermoFisher, LabStorage, Belair, Rankin Biomedical, Source Medical, Mikron and more carry these). Does anyone know how much a new print nozzle and ink from Sakura cost these days? Well that is my three cents... Good luck! ------------------------------ Message: 11 Date: Wed, 9 Jul 2008 14:35:09 -0700 From: "R C" Subject: [Histonet] Shandon vs Sakura block/slide labelers To: histonet@lists.utsouthwestern.edu Message-ID: <2a926e3f0807091435y7df33e3al4be3be07788166ff@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 We're entertaining two products. The Sakura Tissue Tek Auto Write Printer and the Thermo Shandon Microwriter I Cassette labeler. Has anyone had any first hand experience with either brands? A slide labeler is projected in the future as well. Thanks. Rueben Carter ? ------------------------------ From Jackie.O'Connor <@t> abbott.com Thu Jul 10 13:47:34 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Jul 10 13:47:52 2008 Subject: [Histonet] RE: Shandon vs Sakura block/slide labelers In-Reply-To: <504844.75848.qm@web44906.mail.sp1.yahoo.com> Message-ID: We use a Leica slide and a block printer (each unit does one job). We love it. You can program vast quantities of slides or cassettes, print them simultaneously, they are instantly dry . The Leica uses an ink cartridge which is pricey - $400 per cartridge, but prints 60,000 slides or blocks. You can plug in your data to the user friendly screen, then walk away to do something else. Love it. Jack Cates Sent by: histonet-bounces@lists.utsouthwestern.edu 07/10/2008 01:18 PM To histonet@lists.utsouthwestern.edu, ruebenjcarter@gmail.com cc Subject [Histonet] RE: Shandon vs Sakura block/slide labelers Ruben, I have used both in the past at two different employers. I have definitely found that the Shandon (RA Lamb, www.ralamb.com or TBS, www.trianglebiomedical.com ) writer to be far superior. The Shandon print medium/tape is impervious to all reagents and does not require any curing time to "set" and the footprint is much, much smaller. Also the cost of operation is not even close... the tape for the Shandon unit sells for approximately $25 dollars and you can get up to 20,000 cassettes, but the Sakura you will need to purchase ink and replace print nozzles, which can be very costly. I think we found that for the same amount of prints/cassettes the price difference could be up to $1500 dollars/year.... pretty substantial. The way that I have come to to think about this... its sort of similar to the situation that when you purchase your home PC, they almost invariably give you a printer for free and make their money back on the sale of ink and or print heads. Also, with the Sandon unit you will have many more outlets for peripherals, like Foil, cassettes and more. (I have seen PSL, TBS, RA Lamb, ThermoFisher, LabStorage, Belair, Rankin Biomedical, Source Medical, Mikron and more carry these). Does anyone know how much a new print nozzle and ink from Sakura cost these days? Well that is my three cents... Good luck! ------------------------------ Message: 11 Date: Wed, 9 Jul 2008 14:35:09 -0700 From: "R C" Subject: [Histonet] Shandon vs Sakura block/slide labelers To: histonet@lists.utsouthwestern.edu Message-ID: <2a926e3f0807091435y7df33e3al4be3be07788166ff@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 We're entertaining two products. The Sakura Tissue Tek Auto Write Printer and the Thermo Shandon Microwriter I Cassette labeler. Has anyone had any first hand experience with either brands? A slide labeler is projected in the future as well. Thanks. Rueben Carter ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> pathview.com Thu Jul 10 14:03:56 2008 From: mike <@t> pathview.com (Michael Mihalik) Date: Thu Jul 10 14:05:09 2008 Subject: [Histonet] RE: Shandon vs Sakura block/slide labelers In-Reply-To: References: <504844.75848.qm@web44906.mail.sp1.yahoo.com> Message-ID: <8C5D8B3F93874FB8BC66C3CD667D2BBD@MDMM1330> There is certainly the cost factor: initial cost vs consumables. But please keep in mind some other perspectives: 1. Batch vs real time generation of blocks or slides 2. accuracy of printed material: drying time, does the 'ink' scratch or come off, does it need a drying time. 3. 1 dimension vs 2 dimension bar code capability -- this goes along the lines of 'what are you going to do with the printed material' on the blocks or slides. ....just keep all this stuff in mind. Michael Mihalik PathView Systems | office: 207.483.0968 | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Thursday, July 10, 2008 2:48 PM To: Jack Cates Cc: histonet@lists.utsouthwestern.edu; ruebenjcarter@gmail.com; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Shandon vs Sakura block/slide labelers We use a Leica slide and a block printer (each unit does one job). We love it. You can program vast quantities of slides or cassettes, print them simultaneously, they are instantly dry . The Leica uses an ink cartridge which is pricey - $400 per cartridge, but prints 60,000 slides or blocks. You can plug in your data to the user friendly screen, then walk away to do something else. Love it. Jack Cates Sent by: histonet-bounces@lists.utsouthwestern.edu 07/10/2008 01:18 PM To histonet@lists.utsouthwestern.edu, ruebenjcarter@gmail.com cc Subject [Histonet] RE: Shandon vs Sakura block/slide labelers Ruben, I have used both in the past at two different employers. I have definitely found that the Shandon (RA Lamb, www.ralamb.com or TBS, www.trianglebiomedical.com ) writer to be far superior. The Shandon print medium/tape is impervious to all reagents and does not require any curing time to "set" and the footprint is much, much smaller. Also the cost of operation is not even close... the tape for the Shandon unit sells for approximately $25 dollars and you can get up to 20,000 cassettes, but the Sakura you will need to purchase ink and replace print nozzles, which can be very costly. I think we found that for the same amount of prints/cassettes the price difference could be up to $1500 dollars/year.... pretty substantial. The way that I have come to to think about this... its sort of similar to the situation that when you purchase your home PC, they almost invariably give you a printer for free and make their money back on the sale of ink and or print heads. Also, with the Sandon unit you will have many more outlets for peripherals, like Foil, cassettes and more. (I have seen PSL, TBS, RA Lamb, ThermoFisher, LabStorage, Belair, Rankin Biomedical, Source Medical, Mikron and more carry these). Does anyone know how much a new print nozzle and ink from Sakura cost these days? Well that is my three cents... Good luck! ------------------------------ Message: 11 Date: Wed, 9 Jul 2008 14:35:09 -0700 From: "R C" Subject: [Histonet] Shandon vs Sakura block/slide labelers To: histonet@lists.utsouthwestern.edu Message-ID: <2a926e3f0807091435y7df33e3al4be3be07788166ff@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 We're entertaining two products. The Sakura Tissue Tek Auto Write Printer and the Thermo Shandon Microwriter I Cassette labeler. Has anyone had any first hand experience with either brands? A slide labeler is projected in the future as well. Thanks. Rueben Carter ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Jul 10 17:50:51 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Jul 10 17:50:29 2008 Subject: [Histonet] Frozen Section controls References: <904128.32580.qm@web57812.mail.re3.yahoo.com> Message-ID: <004901c8e2df$676c14e0$0302a8c0@yourxhtr8hvc4p> I haven't been flamed in a while. Today seems like a good day. I took the pleasure of pasting these CAP questions to my answer. ANP.21250 Phase II N/A YES NO Examine several prepared slides. Are they of sufficient quality for diagnosis? NOTE: Histopathology slides must be of adequate technical quality to be diagnostically useful. Criteria to evaluate include adequate tissue fixation, thickness of sections, absence of interfering tissue folds and tears, and good staining technique. For hematoxylin and eosin and other routine stains, the patient slide serves as the internal control to ensure adequate staining technique. The sections must be cut from sufficient depth in the block to include the entire tissue plane. ANP.21400 Phase II N/A YES NO Are positive controls run routinely on all special stains, with reactivity results documented, and are they verified for acceptability before reporting results? NOTE: A positive control slide must be run at the same time as any single or group of slides stained with the same special stain. The tissue chosen for the special stain control slide must be appropriate in type and amount. Both the control slide and the test tissue slide must be judged technically acceptable before the results of the special stains are reported. If your metachromatic stain is part of your routine frozen procedure, then it is a routine stain. I mean, you use routinely, don't you? If not, then I would put it in my procedure as a routine stain, make a copy and send it to CAP with your deficiency package. Problem solved. JTT ----- Original Message ----- From: "Sheila Haas" To: Sent: Thursday, July 10, 2008 9:50 AM Subject: [Histonet] Frozen Section controls Here's a new one on me. At recent inspection I was told we should be running a frozen section control slide with our metachromatic stain daily. The inspector was at an off-site facility where we do not have capability of keeping tissue frozen from one day to next not to mention that the metachromatic stain is not permanent (he told me we had to hold slides for 2 years). I also explained that our pathologists do indicate acceptability of stain daily but he said that was not sufficient since the pathologist was looking at patient tissue instead of control tissue. When I explained issue of inability to keep tissue frozen (we have numerous off-site locations) he said to cut an initial, superficial slide of patient tissue to use as control then proceed with patient frozen section after approval of stain. Any one heard of this? Seems a bit much to me. Sheila Haas Laboratory Supervisor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maria.Mejia <@t> ucsf.edu Thu Jul 10 18:38:15 2008 From: Maria.Mejia <@t> ucsf.edu (Mejia, Maria) Date: Thu Jul 10 18:38:25 2008 Subject: [Histonet] need protocol for MHC-11 class antigens: also CD4 & CD8 for free-floating sections Message-ID: <6CF686BD6F24A546B85B24FE3B97864701B79046@EXVS06.net.ucsf.edu> Hello, I'm looking for any protocols for MHC-11 class antigens as well as CD4 & CD8. I'm working on primate 40um free-floating sections fixed in Zamboni fixative. Please provide company information too. Any information anyone can provide will be greatly appreciated. Regards Maria Bartola Mejia Department of Neurosurgery UCSF SF CA 94103 From kgreicar <@t> thepathlab.com Thu Jul 10 22:36:12 2008 From: kgreicar <@t> thepathlab.com (Kipp Greicar) Date: Thu Jul 10 22:36:28 2008 Subject: [Histonet] Tissue tek xpress 50 Message-ID: The standard program for the xpress 50 is 40 minutes/retort for a total of 80 minutes. Is it possible to shorten the time in each retort if we are running a batch of small skins and small biopsy specimens Kipp Greicar Anatomical Pathology Manager The Pathology Lab LakeCharles LA From michelle-griffin-reyes <@t> uiowa.edu Fri Jul 11 08:24:07 2008 From: michelle-griffin-reyes <@t> uiowa.edu (Griffin-Reyes, Michelle A) Date: Fri Jul 11 08:24:19 2008 Subject: [Histonet] Saturated Fat Staining Message-ID: I am having some troubles finding a way to be able to stain saturated lipids in FFPE mouse tissues. Osmium tetroxide and oil red O stain well but they are only showing unsaturated lipids. My lab is looking to see if there is a difference in lipid content of treated mice vs. control mice. So far, we have seen very little variability in the amount of unsaturated lipids during quantification which leads us to believe there may be a difference in saturated fats (based off of human results that we have our mouse model based off of). Does anyone have any suggestions? Thanks so much for the help! Michelle A. Griffin-Reyes Comparative Pathology Laboratory University of Iowa: College of Medicine Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. From rjbuesa <@t> yahoo.com Fri Jul 11 09:04:50 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jul 11 09:04:59 2008 Subject: [Histonet] Saturated Fat Staining In-Reply-To: Message-ID: <255434.1397.qm@web65715.mail.ac4.yahoo.com> The fats in any FFPE tissue have been partially dissolved by the ante-medium, and Oil Red O will not give very good results. Osmium is too toxic and I would avoid its use. I think you could do two things: 1- eliminate the ante-medium and use?dehydration with 2-propanol, followed by a mixture of mineral oil + 2-propanol as ante-medium (before the paraffin), and 2- try Sudan Black. ? I also would stain first a group of untreated mice tissues?first to get a control of the procedure, and use the treated mice afterward. Hope this will help you! Ren? J.? --- On Fri, 7/11/08, Griffin-Reyes, Michelle A wrote: From: Griffin-Reyes, Michelle A Subject: [Histonet] Saturated Fat Staining To: histonet@lists.utsouthwestern.edu Date: Friday, July 11, 2008, 9:24 AM I am having some troubles finding a way to be able to stain saturated lipids in FFPE mouse tissues. Osmium tetroxide and oil red O stain well but they are only showing unsaturated lipids. My lab is looking to see if there is a difference in lipid content of treated mice vs. control mice. So far, we have seen very little variability in the amount of unsaturated lipids during quantification which leads us to believe there may be a difference in saturated fats (based off of human results that we have our mouse model based off of). Does anyone have any suggestions? Thanks so much for the help! Michelle A. Griffin-Reyes Comparative Pathology Laboratory University of Iowa: College of Medicine Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr <@t> comcast.net Fri Jul 11 10:10:27 2008 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Fri Jul 11 10:10:32 2008 Subject: [Histonet] Saturated Fat Staining Message-ID: <071120081510.19764.487777E300087DD400004D3422070032019D09020704040A0105@comcast.net> Michelle, If these are already collected and is a retrospective study, it seems like you are pushing the limitations of histochemical analysis. Unless there are very big physiological differences between control and treated, is hard to see how you can see small differences through a microscope with stains. You said there was "little variability" with unsaturated fats during quantification. I assume that is with a stain. But is there a statistical difference by more precise measurements? If already done, then no suggestions other than what you've had already and other than good luck. But in a different life and faced with such kinds of analysis, we just acknowledged histochemical limitations. Sounds like part of the liver should be cut, weighed, fat extracted, and do something like gas chromatography analysis. Then get precise measurements of saturated and un and other lipids. The human results were surely done with something a bit more precise and quantifiable than an oil red o sta in on a microscopic section? Or maybe do the project in parallel, part for quantifiable analysis and part liver for you done somehow in FFPE. But best wishes for success. Ray Koelling PhenoPath Labs Seattle, WA -------------- Original message ---------------------- From: "Griffin-Reyes, Michelle A" > I am having some troubles finding a way to be able to stain saturated > lipids in FFPE mouse tissues. Osmium tetroxide and oil red O stain well > but they are only showing unsaturated lipids. My lab is looking to see > if there is a difference in lipid content of treated mice vs. control > mice. So far, we have seen very little variability in the amount of > unsaturated lipids during quantification which leads us to believe there > may be a difference in saturated fats (based off of human results that > we have our mouse model based off of). Does anyone have any suggestions? > Thanks so much for the help! > > Michelle A. Griffin-Reyes > Comparative Pathology Laboratory > University of Iowa: College of Medicine > > > > Notice: This UI Health Care e-mail (including attachments) is covered by the > Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and > may be legally privileged. If you are not the intended recipient, you are > hereby notified that any retention, dissemination, distribution, or copying of > this communication is strictly prohibited. Please reply to the sender that you > have received the message in error, then delete it. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ryaskovich <@t> dir.nidcr.nih.gov Fri Jul 11 10:36:57 2008 From: ryaskovich <@t> dir.nidcr.nih.gov (Yaskovich, Ruth A (NIH/NIDCR) [E]) Date: Fri Jul 11 10:37:11 2008 Subject: [Histonet] TRPV-1 Message-ID: Anybody know of a company that makes a good TRPV-1 antibody that works on Rat tissues, ganlia, and spinal cord? I had a nice one and it's no longer available. Thanks in advance. Ruth Yaskovich National Institutes of Health National Institute of Dental and Craniofacial Research Neurobiology and Pain Therapeutics From tifei <@t> foxmail.com Fri Jul 11 10:41:40 2008 From: tifei <@t> foxmail.com (tf) Date: Fri Jul 11 10:42:26 2008 Subject: [Histonet] Anterograde tracing References: <071120081510.19764.487777E300087DD400004D3422070032019D09020704040A0105@comcast.net> Message-ID: <200807112341346772475@foxmail.com> I just want to discuss the best anterograde tracer. BDA, PHA-L, CTB-FITC, CTB-Rhodamine, WGA-HRP, HRP, and viral vectors all could be anterograde tracer. However, BDA is diffusible like all fluroscent dyes, does not have the necessary direction. CTB-conjugated sereis have limited diffusion ability when injected with pressure, just like PHA-L, thus are not good in labeling a larger group of neurons when single rather multiple injections is required. WGA-HRP & HRP both antero & retrogradely transported, HRP & virus even transynaptically. So, any one has other ideas? Such as shorten the period after injection to reduce the diffusion, or using immunodetection to visualize the CTB-FITC, for example? 2008-07-11 tf From rachelr <@t> nei.nih.gov Fri Jul 11 10:56:18 2008 From: rachelr <@t> nei.nih.gov (Rachel, Rivka (NIH/NEI) [E]) Date: Fri Jul 11 11:00:59 2008 Subject: [Histonet] Anterograde tracing References: <071120081510.19764.487777E300087DD400004D3422070032019D09020704040A0105@comcast.net> <200807112341346772475@foxmail.com> Message-ID: What type of tissue are you trying to trace? If fixed, immature rodent tissue, have you considered one of the fluorescent tracers such as DiI, DiO, DiD, etc. available through Molecular Probes (Invitrogen)? DiI (red fluorescence) is the brightest. These dyes don't work well in adult tissue, however. Rivka Rivka A. Rachel, MD, PhD Staff Scientist, National Eye Institute Neurobiology-Neurodegeneration and Repair Laboratory Tel: 301 443-4906 -----Original Message----- From: tf [mailto:tifei@foxmail.com] Sent: Fri 7/11/2008 11:41 AM To: histonet Subject: [Histonet] Anterograde tracing I just want to discuss the best anterograde tracer. BDA, PHA-L, CTB-FITC, CTB-Rhodamine, WGA-HRP, HRP, and viral vectors all could be anterograde tracer. However, BDA is diffusible like all fluroscent dyes, does not have the necessary direction. CTB-conjugated sereis have limited diffusion ability when injected with pressure, just like PHA-L, thus are not good in labeling a larger group of neurons when single rather multiple injections is required. WGA-HRP & HRP both antero & retrogradely transported, HRP & virus even transynaptically. So, any one has other ideas? Such as shorten the period after injection to reduce the diffusion, or using immunodetection to visualize the CTB-FITC, for example? 2008-07-11 tf _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tifei <@t> foxmail.com Fri Jul 11 11:44:39 2008 From: tifei <@t> foxmail.com (tf) Date: Fri Jul 11 11:45:17 2008 Subject: [Histonet] Anterograde tracing References: <071120081510.19764.487777E300087DD400004D3422070032019D09020704040A0105@comcast.net>, <200807112341346772475@foxmail.com>, Message-ID: <200807120044345016582@foxmail.com> RGVhciBSYWNoZWw6DQoNClN1cmVseSBJIGhhdmUgY29uc2lkZXJlZCB0aGF0Lg0KDQpJIGFtIGFj dHVhbGx5IHdvcmtpbmcgb24gcmVnZW5lcmF0aW9uLg0KQWxzbywgZHllcyBhcmUgdmVyc2F0aWxl IGFuZCBnbyBldmVyeXdoZXJlIGFzIGxvbmcgYXMgbWVtYnJhbmUgcGVybWl0cy4gSXQgZG9lcyBu b3QgaGF2ZSB0aGUgcmVzdHJpY3RlZCBsYWJlbGluZyBvZiBvZiBvbmUgYmF0Y2ggb2YgcHJvamVj dGlvbiBhbmQgbWF5IGRpZmZ1c2UgYSBsb3Qgd2hlbiB0cmFjaW5nIHRoZSB0cmFuc2VjdGlvbiBz aXRlLiBBbm90aGVyIHByb2JsZW0geW91IGhhdmUgbWVudGlvbmVkIGlzIHRoYXQgRGlJIGRvZXMg bm90IGRpZmZ1c2Ugd2VsbCBpbiBhZHVsdCB0aXNzdWUsIGFuZCB0aHVzIHVzZWQgbW9yZSBpbiBk ZXZlbG9wbWVudGFsIHN0dWRpZXMuDQoNCnRoYW5rcyBhIGxvdA0KDQoyMDA4LTA3LTEyIA0KDQoN Cg0KdGYgDQoNCg0KDQq3orz+yMujuiBSYWNoZWwsIFJpdmthIChOSUgvTkVJKSBbRV0gDQq3osvN yrG85KO6IDIwMDgtMDctMTIgIDAwOjAwOjQ5IA0KytW8/sjLo7ogdGlmZWlAZm94bWFpbC5jb207 IGhpc3RvbmV0IA0Ks63LzaO6IA0K1vfM4qO6IFJFOiBbSGlzdG9uZXRdIEFudGVyb2dyYWRlIHRy YWNpbmcgDQogDQpXaGF0IHR5cGUgb2YgdGlzc3VlIGFyZSB5b3UgdHJ5aW5nIHRvIHRyYWNlPyAg SWYgZml4ZWQsIGltbWF0dXJlIHJvZGVudCB0aXNzdWUsIGhhdmUgeW91IGNvbnNpZGVyZWQgb25l IG9mIHRoZSBmbHVvcmVzY2VudCB0cmFjZXJzIHN1Y2ggYXMgRGlJLCBEaU8sIERpRCwgZXRjLiBh dmFpbGFibGUgdGhyb3VnaCBNb2xlY3VsYXIgUHJvYmVzIChJbnZpdHJvZ2VuKT8gIERpSSAocmVk IGZsdW9yZXNjZW5jZSkgaXMgdGhlIGJyaWdodGVzdC4gIFRoZXNlIGR5ZXMgZG9uJ3Qgd29yayB3 ZWxsIGluIGFkdWx0IHRpc3N1ZSwgaG93ZXZlci4NClJpdmthDQpSaXZrYSBBLiBSYWNoZWwsIE1E LCBQaEQNClN0YWZmIFNjaWVudGlzdCwgTmF0aW9uYWwgRXllIEluc3RpdHV0ZQ0KTmV1cm9iaW9s b2d5LU5ldXJvZGVnZW5lcmF0aW9uIGFuZCBSZXBhaXIgTGFib3JhdG9yeQ0KVGVsOiAzMDEgNDQz LTQ5MDYNCi0tLS0tT3JpZ2luYWwgTWVzc2FnZS0tLS0tDQpGcm9tOiB0ZiBbbWFpbHRvOnRpZmVp QGZveG1haWwuY29tXQ0KU2VudDogRnJpIDcvMTEvMjAwOCAxMTo0MSBBTQ0KVG86IGhpc3RvbmV0 DQpTdWJqZWN0OiBbSGlzdG9uZXRdIEFudGVyb2dyYWRlIHRyYWNpbmcNCg0KSSBqdXN0IHdhbnQg dG8gZGlzY3VzcyB0aGUgYmVzdCBhbnRlcm9ncmFkZSB0cmFjZXIuDQpCREEsIFBIQS1MLCBDVEIt RklUQywgQ1RCLVJob2RhbWluZSwgV0dBLUhSUCwgSFJQLCBhbmQgdmlyYWwgdmVjdG9ycyBhbGwg Y291bGQgYmUgYW50ZXJvZ3JhZGUgdHJhY2VyLg0KSG93ZXZlciwgQkRBIGlzIGRpZmZ1c2libGUg bGlrZSBhbGwgZmx1cm9zY2VudCBkeWVzLCBkb2VzIG5vdCBoYXZlIHRoZSBuZWNlc3NhcnkgZGly ZWN0aW9uLg0KQ1RCLWNvbmp1Z2F0ZWQgc2VyZWlzIGhhdmUgbGltaXRlZCBkaWZmdXNpb24gYWJp bGl0eSB3aGVuIGluamVjdGVkIHdpdGggcHJlc3N1cmUsIGp1c3QgbGlrZSBQSEEtTCwgdGh1cyBh cmUgbm90IGdvb2QgaW4gbGFiZWxpbmcgYSBsYXJnZXIgZ3JvdXAgb2YgbmV1cm9ucyB3aGVuIHNp bmdsZSByYXRoZXIgbXVsdGlwbGUgaW5qZWN0aW9ucyBpcyByZXF1aXJlZC4NCldHQS1IUlAgJiBI UlAgYm90aCBhbnRlcm8gJiByZXRyb2dyYWRlbHkgdHJhbnNwb3J0ZWQsIEhSUCAmIHZpcnVzIGV2 ZW4gdHJhbnN5bmFwdGljYWxseS4NClNvLCBhbnkgb25lIGhhcyBvdGhlciBpZGVhcz8gU3VjaCBh cyBzaG9ydGVuIHRoZSBwZXJpb2QgYWZ0ZXIgaW5qZWN0aW9uIHRvIHJlZHVjZSB0aGUgZGlmZnVz aW9uLCBvciB1c2luZyBpbW11bm9kZXRlY3Rpb24gdG8gdmlzdWFsaXplIHRoZSBDVEItRklUQywg Zm9yIGV4YW1wbGU/DQoyMDA4LTA3LTExIA0KdGYgDQpfX19fX19fX19fX19fX19fX19fX19fX19f X19fX19fX19fX19fX19fX19fX19fXw0KSGlzdG9uZXQgbWFpbGluZyBsaXN0DQpIaXN0b25ldEBs aXN0cy51dHNvdXRod2VzdGVybi5lZHUNCmh0dHA6Ly9saXN0cy51dHNvdXRod2VzdGVybi5lZHUv bWFpbG1hbi9saXN0aW5mby9oaXN0b25ldA0K From liz <@t> premierlab.com Fri Jul 11 11:58:52 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Jul 11 11:58:57 2008 Subject: [Histonet] rabbit BMP2 Message-ID: Hello all Is anyone out there aware of an anti-BMP2 antibody that will detect native rabbit BMP2. Any advice would be appreciated. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 From liz <@t> premierlab.com Fri Jul 11 12:02:26 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Jul 11 12:02:29 2008 Subject: [Histonet] rabbit CD4 and CD8 paraffin Message-ID: Hello again Can anyone help me out with finding antibodies to rabbit CD4 and CD8 that will work in paraffin sections. From my research it looks like CD4 will only work in frozen sections. I have found a paper that used a GeneTex CD8 antibody on paraffin sections but the spec sheet states the applications are for Flow and FACS. Any help is appreciated. I know that this has been asked before but I can't seem to get on the archives page. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 From trathborne <@t> somerset-healthcare.com Fri Jul 11 12:08:34 2008 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Fri Jul 11 12:08:39 2008 Subject: [Histonet] rabbit CD4 and CD8 paraffin In-Reply-To: Message-ID: Liz, Leica markets Novocastra CD4 and CD8 for frozen or paraffin. They also offer a duo pack which includes both. Toni -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Liz Chlipala Sent: Friday, July 11, 2008 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] rabbit CD4 and CD8 paraffin Hello again Can anyone help me out with finding antibodies to rabbit CD4 and CD8 that will work in paraffin sections. From my research it looks like CD4 will only work in frozen sections. I have found a paper that used a GeneTex CD8 antibody on paraffin sections but the spec sheet states the applications are for Flow and FACS. Any help is appreciated. I know that this has been asked before but I can't seem to get on the archives page. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. From Sarah_Mack <@t> urmc.rochester.edu Fri Jul 11 12:22:48 2008 From: Sarah_Mack <@t> urmc.rochester.edu (Mack, Sarah A) Date: Fri Jul 11 12:24:43 2008 Subject: [Histonet] Gr 1 IHC Message-ID: Hello everyone. I was wondering if anyone has had successful results when doing IHC with Gr 1 on 4% PFA paraffin embedded tissue. We have been picking up non specific staining that does not seem to be andogenous. Any suggestions will be GREATLY appreciated! Thank you. Sarah A. Mack Center for Pediatric Biomedical Research University of Rochester Medical Center 575 Elmwood Avenue, MRBX 1.11301 Rochester, NY 14642 Tel (585) 275-5073 Fax (585) 276-0232 From bdelescavage <@t> cellnetix.com Fri Jul 11 12:46:43 2008 From: bdelescavage <@t> cellnetix.com (Beth Delescavage) Date: Fri Jul 11 12:48:19 2008 Subject: [Histonet] Slide and Block scanning Message-ID: Happy Friday Everyone~ Has anyone read or written any papers on scanning slides and blocks for Histology laboratory practices? Could you send me the references to these articles if they exist? Thanks~ Beth Beth Delescavage, BS, HTL (ASCP) QIHC DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From mike <@t> pathview.com Fri Jul 11 13:02:12 2008 From: mike <@t> pathview.com (Michael Mihalik) Date: Fri Jul 11 13:03:04 2008 Subject: [Histonet] Slide and Block scanning In-Reply-To: References: Message-ID: <8BF184EC53454A1B8C587D48ED885B14@MDMM1330> >From what perspective do you mean? Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth Delescavage Sent: Friday, July 11, 2008 1:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide and Block scanning Happy Friday Everyone~ Has anyone read or written any papers on scanning slides and blocks for Histology laboratory practices? Could you send me the references to these articles if they exist? Thanks~ Beth Beth Delescavage, BS, HTL (ASCP) QIHC DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bdelescavage <@t> cellnetix.com Fri Jul 11 13:02:01 2008 From: bdelescavage <@t> cellnetix.com (Beth Delescavage) Date: Fri Jul 11 13:03:31 2008 Subject: [Histonet] Slide and Block scanning In-Reply-To: References: Message-ID: Hi Mike~ >From the over all prospective of lean and laboratory practices, does that make sense? Thanks~ Beth Beth Delescavage, BS, HTL (ASCP) QIHC -----Original Message----- From: Michael Mihalik [mailto:m.mihalik@comcast.net] Sent: Friday, July 11, 2008 10:56 AM To: Beth Delescavage; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide and Block scanning >From what perspective do you mean? Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth Delescavage Sent: Friday, July 11, 2008 1:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide and Block scanning Happy Friday Everyone~ Has anyone read or written any papers on scanning slides and blocks for Histology laboratory practices? Could you send me the references to these articles if they exist? Thanks~ Beth Beth Delescavage, BS, HTL (ASCP) QIHC DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From Reuel.Cornelia <@t> tsrh.org Fri Jul 11 13:27:41 2008 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Fri Jul 11 13:28:18 2008 Subject: [Histonet] job in southern California Message-ID: <48775FCD020000C50003BED9@nwcl02.tsrh.org> I have a friend who is looking for a job in southern California. he wanted clinical/ research pathology lab with Electron Microscopy and Immunohistochemistry. if you need one, please let me know. Thank you. Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 ******************************************************************************************************************* Texas Scottish Rite Hospital for Children is one of the nation's leading pediatric centers for the treatment of orthopedic conditions, certain related neurological disorders and learning disorders, such as dyslexia. This email transmission and/or its attachments may contain confidential health information, intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing it to any other party unless required to do so by law and is required to delete/destroy the information after its stated need has been fulfilled. If you are not the intended recipient, any disclosure, copying, distribution or action taken in reliance on the contents of this email transmission is prohibited. If you have received this information in error, please notify the sender immediately and delete this information. We appreciate your efforts to protect the children's confidential information. ******************************************************************************************************************* From schaundrawalton <@t> yahoo.com Fri Jul 11 13:57:21 2008 From: schaundrawalton <@t> yahoo.com (Schaundra Walton) Date: Fri Jul 11 13:57:25 2008 Subject: [Histonet] RE: Tissue Flotation Baths Message-ID: <589999.17486.qm@web58915.mail.re1.yahoo.com> Thanks to everyone who sent information about the tissue flotation baths and where to get them!? It was very helpful! ? -Schaundra Walton BS, HTL(ASCP) Histology Supervisor Swedish American Hospital Rockford, IL From Heather.D.Renko <@t> osfhealthcare.org Fri Jul 11 14:00:47 2008 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Fri Jul 11 14:01:10 2008 Subject: [Histonet] Re: Leica IPC Cassette Writer Message-ID: <40026EDDE64CDA47AB382C52619ACD3C0C44A022@pmc-rfd-mx01.intranet.osfnet.org> I just recently purchased a Leica IPC Cassette writer after looking at many brands with the Thermo being one of the finalists. My decision was based on ease of integration down the road and I really like the engineering behind the design. Of course everyone has some hiccups with any major purchase but, overall they are both excellent instruments and from personal experience I would recommend the Leica IPC for quality and the in house technical support that I was given when I recently had some issues. It's a good reliable instrument and I really like the functionality of the instrument within my facility . Lastly, lets not forget our patient safety goals are calling for "two identifiers", the Leica IPC does this very nicely. TGIF Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From sjchtascp <@t> yahoo.com Fri Jul 11 14:26:10 2008 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Fri Jul 11 14:26:13 2008 Subject: [Histonet] Looking for a Job Message-ID: <216170.27073.qm@web38207.mail.mud.yahoo.com> I'm from So. WI, just south of Madison and north of Rockford, Ill.? FT, PT or PRN would be fine. ? Thanks, ? Steve From mike <@t> pathview.com Fri Jul 11 15:02:57 2008 From: mike <@t> pathview.com (Michael Mihalik) Date: Fri Jul 11 15:03:51 2008 Subject: [Histonet] Re: Leica IPC Cassette Writer In-Reply-To: <40026EDDE64CDA47AB382C52619ACD3C0C44A022@pmc-rfd-mx01.intranet.osfnet.org> References: <40026EDDE64CDA47AB382C52619ACD3C0C44A022@pmc-rfd-mx01.intranet.osfnet.org> Message-ID: <2EAACDAC94E14888A852B7CFDCC4BF00@MDMM1330> Heather, how quickly will the Leica print a cassette? Does it have a 2D barcode on it? Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Renko, Heather D. Sent: Friday, July 11, 2008 3:01 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Leica IPC Cassette Writer I just recently purchased a Leica IPC Cassette writer after looking at many brands with the Thermo being one of the finalists. My decision was based on ease of integration down the road and I really like the engineering behind the design. Of course everyone has some hiccups with any major purchase but, overall they are both excellent instruments and from personal experience I would recommend the Leica IPC for quality and the in house technical support that I was given when I recently had some issues. It's a good reliable instrument and I really like the functionality of the instrument within my facility . Lastly, lets not forget our patient safety goals are calling for "two identifiers", the Leica IPC does this very nicely. TGIF Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================ == The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================ == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From snm320 <@t> comcast.net Fri Jul 11 15:19:43 2008 From: snm320 <@t> comcast.net (Henderson) Date: Fri Jul 11 15:19:53 2008 Subject: [Histonet] Re: Tracking Floaters Message-ID: <3C30326E-ADC8-4355-997D-E1DEA34347C7@comcast.net> We had the same problem at my previous job. The PA was grossing smaller specimens and placing the cassettes into a large container with formalin. Then the PA was grossing the placentas and adding these cassettes into the same holding container. The problem was fixed by keeping the placenta cassettes in a separate container. At the end of the day, everything was loaded into the processing basket and placed into the processor. This seem to have eliminated any further contamination with placenta parts. Please note that we did not at any time mix the formalin of the containers. > Message: 1 > Date: Thu, 10 Jul 2008 13:08:58 -0400 > From: "Cynthia Robinson" > Subject: RE: [Histonet] Tracking Floaters > To: "Laurie Colbert" , > "Histonet" , > > Message-ID: <4875FBDA.59BC.00AF.0@mercyhealth.com> > Content-Type: text/plain; charset=US-ASCII > > We have been addressing the floater issue. We found that changing > to smaller mesh type cassettes has helped but not eliminated the > problem even with meticulous care at embedding station (wiping and > changing pick ups in between each specimen, pick ups without any > ridges, etc). Our paths see tiny, like 10 or less clusters of cells > from placenta usually, on some slides but when we cut deeper > sections the floaters are gone. Usually it seems to be bone > sections which will 'hold onto' these tiny groups of cells. We are > continuing to try to fix this issue and would like to hear other > suggestions. > > Thanks. > > Cindi Robinson HT(ASCP) > MMC-Sioux City > Dunes Medical Laboratories > 350 W Anchor Dr > Dakota Dunes SD 57049 From tmalloy77 <@t> hotmail.com Fri Jul 11 18:23:47 2008 From: tmalloy77 <@t> hotmail.com (TIMOTHY MALLOY) Date: Fri Jul 11 18:23:52 2008 Subject: [Histonet] HISTOLOGY ASCP EXAM Message-ID: I am a fresh graduate student Histotechnician awaiting to take the ASCP exam. Can anybody help with any web sites that display photos of histology special stains in human tissue. tmalloy77@hotmail.com 1-401-965-4935 This email communication may contain CONFIDENTIAL INFORMATION WHICH ALSO MAY BE LEGALLY PRIVILEGED and is intended only for the use of the intended recipients identified above. If you are not the intended recipient of this communication, you are hereby notified that any unauthorized review, use, dissemination, distribution, downloading, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately notify us by reply email, delete the communication and destroy all copies. From tmalloy77 <@t> hotmail.com Fri Jul 11 18:41:39 2008 From: tmalloy77 <@t> hotmail.com (TIMOTHY MALLOY) Date: Fri Jul 11 18:41:44 2008 Subject: [Histonet] HISTOLOGY ASCP EXAM Message-ID: I am a new Histotech awaiting the ASCP Exam. Has anyone out there come across any good web sites that display human tissue on " Special Stains " Tim Malloy Histotechnician tmalloy77@hotmail.com tmalloy77@hotmail.com 1-401-965-4935 This email communication may contain CONFIDENTIAL INFORMATION WHICH ALSO MAY BE LEGALLY PRIVILEGED and is intended only for the use of the intended recipients identified above. If you are not the intended recipient of this communication, you are hereby notified that any unauthorized review, use, dissemination, distribution, downloading, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately notify us by reply email, delete the communication and destroy all copies. From joelleweaver <@t> hotmail.com Fri Jul 11 19:29:00 2008 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri Jul 11 19:29:05 2008 Subject: [Histonet] HISTOLOGY ASCP EXAM Message-ID: Hi TimothyThese sites may be good for review. The 1st has a 'histology learning' on the left side and there are several things that might help with study. The lumen site has practicals, more probably ID than staining, but when I took the exam it helped me as much to be able to identify the tissue as the stain, since procedures and counterstains vary so. Sometimes the resolution on the monitor is so-so with the CAT exams, so maybe it might help? The IHC world is a good site, but also has some specials on it that you can look at. There are a lot out there, but I had these book-marked. Good luck on your test.Joelle Weaver BA, HTL(ASCP) http://www.histology-world.com/http://www.lumen.luc.edu/lumen/MedEd/Histo/frames/histo_frames.htmlhttp://www.ihcworld.com/imagegallery/displayimage.php?album=4&pos=3> From: tmalloy77@hotmail.com> To: histonet@lists.utsouthwestern.edu> Date: Fri, 11 Jul 2008 19:23:47 -0400> Subject: [Histonet] HISTOLOGY ASCP EXAM> > > I am a fresh graduate student Histotechnician awaiting to take the ASCP exam. Can anybody help with any web sites that display photos of histology special stains in human tissue.> > tmalloy77@hotmail.com 1-401-965-4935> This email communication may contain CONFIDENTIAL INFORMATION WHICH ALSO MAY BE LEGALLY PRIVILEGED and is intended only for the use of the intended recipients identified above. If you are not the intended recipient of this communication, you are hereby notified that any unauthorized review, use, dissemination, distribution, downloading, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately notify us by reply email, delete the communication and destroy all copies._______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ It?s a talkathon ? but it?s not just talk. http://www.imtalkathon.com/?source=EML_WLH_Talkathon_JustTalk From podpath <@t> bellsouth.net Sat Jul 12 09:37:55 2008 From: podpath <@t> bellsouth.net (podpath@bellsouth.net) Date: Sat Jul 12 09:37:59 2008 Subject: [Histonet] Positions in Georgia Message-ID: <071220081437.12590.4878C1C2000D1E010000312E22230706129B0A02D2089B9A019C04040A0DBF089B0E9F0B019F@att.net> Exciting job opportunity with a new reference lab in Alpharetta, GA. Lab will open late summer to early fall 2008. Applicants should be HTL or HT certified. Job description includes general histology including experience with manual special stains and immunos. Specimens include primarily Dematopathology, but some smaller biopsy samples will be recieved, as well. The lab is offering a very competitive benefits package with sign on bonus and very congenial work environment. Other positions also available include data entry and pathology/medical transcription. Interested parties should respond with resume to podpath@bellsouth.net. If you want to be part of something new with excellent growth potential and a family like atmosphere, then this job may be for you. From tifei <@t> foxmail.com Sat Jul 12 10:57:49 2008 From: tifei <@t> foxmail.com (tf) Date: Sat Jul 12 10:58:13 2008 Subject: [Histonet] Double immunostaining with two same source 1st Antibody Message-ID: <200807122357446234117@foxmail.com> Hi all Just wonder any one of you tried to perform IHC using two primary antibodies from same source, mice, for example? The process is that Mice anti-A (1st IHC)->secondary antibody labeled with GREEN fluroscence->wash -> Mice anti-B (2nd IHC)-> Red fluroscence then there's no cross reaction. thx. 2008-07-12 tf From pruegg <@t> ihctech.net Sat Jul 12 13:07:48 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Sat Jul 12 13:09:23 2008 Subject: [Histonet] Double immunostaining with two same source 1st Antibody In-Reply-To: <200807122357446234117@foxmail.com> Message-ID: <200807121807.m6CI7iTn091359@pro42.abac.com> You can still have issues with the second red detection trying to stick to the first AB unless you do some very aggressive blocking. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of tf Sent: Saturday, July 12, 2008 9:58 AM To: histonet Subject: [Histonet] Double immunostaining with two same source 1st Antibody Hi all Just wonder any one of you tried to perform IHC using two primary antibodies from same source, mice, for example? The process is that Mice anti-A (1st IHC)->secondary antibody labeled with GREEN fluroscence->wash -> Mice anti-B (2nd IHC)-> Red fluroscence then there's no cross reaction. thx. 2008-07-12 tf _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ldunfee <@t> seattlecca.org Sun Jul 13 15:00:39 2008 From: ldunfee <@t> seattlecca.org (Dunfee, LuWanda R) Date: Sun Jul 13 15:00:47 2008 Subject: [Histonet] Reticular stain Message-ID: Hello, I am working on the reticular stain that we are currently using. We are using a modified version of the Gordon and Sweet's stain. It is not selective enough, and it is picking up other collagen fibers. I was wondering if anyone had any suggestions. Thank You, LuWanda Seattle Cancer Care Alliance This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 288-6266, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. From rjbuesa <@t> yahoo.com Sun Jul 13 15:35:37 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Jul 13 15:35:41 2008 Subject: [Histonet] Reticular stain Message-ID: <48288.32832.qm@web65702.mail.ac4.yahoo.com> Try Gomori's method. Ren? J. --- On Sun, 7/13/08, Dunfee, LuWanda R wrote: From: Dunfee, LuWanda R Subject: [Histonet] Reticular stain To: histonet@lists.utsouthwestern.edu Date: Sunday, July 13, 2008, 4:00 PM Hello, I am working on the reticular stain that we are currently using. We are using a modified version of the Gordon and Sweet's stain. It is not selective enough, and it is picking up other collagen fibers. I was wondering if anyone had any suggestions. Thank You, LuWanda Seattle Cancer Care Alliance This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 288-6266, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Sun Jul 13 18:01:21 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Sun Jul 13 18:01:23 2008 Subject: [Histonet] Reply double immunostaining on same species, mouse Message-ID: <000e01c8e53c$5dfc8d90$6501a8c0@DHXTS541> We do double and triple immunfluorescence staining on mouse tissues with all antibodies raised in same host species only on mouse tissues, rat antiMouse. It takes some careful blocking and organization, but it works beautifully. Please contact me and I will discuss the details on how to set up the staining, but supply some details on what you want to see as green or red fluorescence. This is only done on frozen sections and NOT on formalin fixed paraffin embedded tissue to eliminate autofluorescence. There are several ways to set this up, and we do use some biotinylated primary antibodies along with Molecular Probes Streptavidin Alexa fluor dyes. Gayle M. CallisHTL/HT/MT(ASCP)Bozeman MT You can still have issues with the second red detection trying to stick to the first AB unless you do some very aggressive blocking. Patsy -----Original Message----- Sent: Saturday, July 12, 2008 9:58 AM To: histonet Subject: [Histonet] Double immunostaining with two same source 1st Antibody Hi all Just wonder any one of you tried to perform IHC using two primary antibodies from same source, mice, for example? The process is that Mice anti-A (1st IHC)->secondary antibody labeled with GREEN fluroscence->wash -> Mice anti-B (2nd IHC)-> Red fluroscence then there's no cross reaction. thx. 2008-07-12 tf _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- << Previous Message | Next Message >> From tifei <@t> foxmail.com Sun Jul 13 21:02:23 2008 From: tifei <@t> foxmail.com (tf) Date: Sun Jul 13 21:03:17 2008 Subject: [Histonet] Double immunostaining with two same source 1st Antibody References: <003301c8e51e$5204b940$e395c5d8@NEIL> Message-ID: <200807141002185117220@foxmail.com> U29tZSBvbmUgc3VnZ2VzdGVkIGEgImNpdHJhdGUgYnVmZmVyIiB0cmVhdG1lbnQgYmVmb3JlIDJu 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References: Message-ID: <200807141008330513746@foxmail.com> VGhhbmtzIHZlcnkgbXVjaCBJZ29yLg0KDQpJJ2QgbGlrZSB0byBzaGFyZSB0aGUgaW5mb3JtYXRp b24gdG8gb3RoZXIgcGVvcGxlIHdobyBhcmUgaW50ZXJlc3RlZCBpbiB0aGlzLg0KDQogDQoid3d3 LmphY2tzb25pbW11bm8uY29tIA0KQ2hlY2sgb3V0IHRoZWlyIHdlYiBzaXRlLXRlY2huaWNhbCBw YWdlcy1zdGFpbmluZyB3aXRoIDIgQWJzIGZyb20gc2FtZQ0Kc291cmNlcy4gVGhlaXIgcHJvdG9j b2xzIGFyZSBnb29kIGFuZCB0aGV5IGRvIHdvcmsNCkx1Y2sNCmlnb3IiDQoNCg0KMjAwOC0wNy0x NCANCg0KDQoNCnRmIA0KDQoNCg0Kt6K8/sjLo7ogSWdvciBOYXNvbmtpbiANCreiy83Ksbzko7og MjAwOC0wNy0xNCAgMTA6MDY6NTUgDQrK1bz+yMujuiB0aWZlaUBmb3htYWlsLmNvbSANCrOty82j uiANCtb3zOKjuiBSZTogW0hpc3RvbmV0XSBEb3VibGUgaW1tdW5vc3RhaW5pbmcgd2l0aCB0d28g c2FtZSBzb3VyY2UgMXN0QW50aWJvZHkgDQogDQpXd3cuamFja3NvbmltbXVuby5jb20NCkNoZWNr IG91dCB0aGVpciB3ZWIgc2l0ZS10ZWNobmljYWwgcGFnZXMtc3RhaW5pbmcgd2l0aCAyIEFicyBm cm9tIHNhbWUNCnNvdXJjZXMuIFRoZWlyIHByb3RvY29scyBhcmUgZ29vZCBhbmQgdGhleSBkbyB3 b3JrDQpMdWNrDQppZ29yDQpPbiA3LzEzLzA4IDEwOjAyIFBNLCAidGYiIDx0aWZlaUBmb3htYWls 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<200807120044345016582@foxmail.com> Message-ID: <200807141011050118208@foxmail.com> SGkgYWxsIGFnYWluLg0KSnVzdCB3b25kZXIgYW55b25lIHRyaWVkIFdHQS1IUlA/DQoNCml0IHNl ZW1zIHRoYXQgV0dBLWhSUCBoYXMgYSBnb29kIGRpZmZ1c2lvbiBhYmlsaXR5LCwgbWFraW5nIGl0 IGlkZWFsIGZvciBsb2NhbCBwcmVzc3VyZSBpbmplY3Rpb24gKHF1aXRlIHNpbXBsZSBtZXRob2Qp LCB3aGljaCBQSEEtTCBkb2VzIGJhZC4NCmFuZCB5b3UgY2FuIGRldGVjdCBpdCB3ZWxsLg0KDQph bnlvbmUgaGF2ZSB3b3JrZWQgd2l0aCB0aGlzPw0KDQp0aHggDQoNCjIwMDgtMDctMTQgDQoNCg0K DQp0ZiANCg0KDQoNCreivP7Iy6O6IHRmIA0Kt6LLzcqxvOSjuiAyMDA4LTA3LTEyICAwMDo1MDoy OSANCsrVvP7Iy6O6IFJhY2hlbCwgUml2a2EgKE5JSC9ORUkpIFtFXTsgaGlzdG9uZXQgDQqzrcvN o7ogDQrW98zio7ogUmU6IFJFOiBbSGlzdG9uZXRdIEFudGVyb2dyYWRlIHRyYWNpbmcgDQogDQpE ZWFyIFJhY2hlbDoNClN1cmVseSBJIGhhdmUgY29uc2lkZXJlZCB0aGF0Lg0KSSBhbSBhY3R1YWxs eSB3b3JraW5nIG9uIHJlZ2VuZXJhdGlvbi4NCkFsc28sIGR5ZXMgYXJlIHZlcnNhdGlsZSBhbmQg Z28gZXZlcnl3aGVyZSBhcyBsb25nIGFzIG1lbWJyYW5lIHBlcm1pdHMuIEl0IGRvZXMgbm90IGhh 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artefact holes on edge of cortex in mouse brain sections Message-ID: <3DBACD0C44A1CF429431C0C6DC3E81690324DA08@crc_exchange.rcsi-internal.ie> Hi all, I was looking for info on troubleshooting what I think is freezing artefact holes (FA) in our tissue sections. It is quite specific to the edge of the brain sections predominantly around the most superficial layers of the cortex and most obvious in the most lateral aspects of the cortex in coronal sections. Brains were fixed in 4% PFA following cervical dislocation and dissection. They were fixed in graded sucrose concentrations of 10%, 20% and then 30% over 3 days followed by rapid freezing in isopentane and dry ice. These brains are stored at -80 until cryostating coronal sections (10 microns thick). The deeper brain structures are perfectly intact and free from FA holes. Has anyone observed this before and do you think this is FA or something else? I can send on a H&E pic if it proves helpful. It might be a bizarre question but could there be anything to do with the cryostat that could be causing this!?! Regards, ?ine ?ine Behan, PhD Department of Psychiatry, Royal College of Surgeons In Ireland, Smurfit Building, Beaumont Hospital, Dublin 9. Ph: +353-1-809-3857/3798 Fx: +353-1-809-3741 From tifei <@t> foxmail.com Mon Jul 14 09:36:46 2008 From: tifei <@t> foxmail.com (tf) Date: Mon Jul 14 09:37:15 2008 Subject: [Histonet] RE: freezing artefact holes on edge of cortex in mousebrain sections References: <3DBACD0C44A1CF429431C0C6DC3E81690324DA08@crc_exchange.rcsi-internal.ie> Message-ID: <200807142236417873896@foxmail.com> RGVhciBBaW5lOg0KDQpZZXMgdGhhdCdzIGEgY29tbW9uIHByb2JsZW0gZm9yIGNyeW9zdGF0IHNl Y3Rpb25zLg0KDQpEaWZmZXJlbnQgcGVvcGxlIGluIG15IGxhYiBoYXMgcXVpdGUgZGl2ZXJzZSBy ZXNwb25zZSB0byB0aGlzLg0KDQpTb21lIHN1Z2dlc3RlZCB0aGF0IHlvdSBjYW4gbGVhdmUgdGhl IGJyYWluIGluIE8uQy5UIGJlZm9yZSBwdXQgdGhlbSBpbiB0byBmcmVlemVyIGZvciAzLTYgaG91 cnMsIGFuZCB0aGUgTy5DLlQgd2lsbCAiZW50ZXIiIHRoZSBicmFpbiB0aXNzdWUuIFRoZW4gbm8g ImNoZWVzZSIuDQoNClNvbWUgb3RoZXIgcGVvcGxlIHRyaWVkIHRoZSBvdGhlciBoYWxmIG9mIHRo ZSBicmFpbiBvbiBtaWNyb3RvbWUsIHRvIHNlZSBpZiB0aGlzIGhlbHBzLiBUbyBvdXIgc3VycHJp c2UsIHRoZXNlIGFyZSBubyBzdWNoIGhvbGxvdyBjYXZpdGllcyBpbiBtaWNyb3RvbWUtbWFkZSBz 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b3ZpZGUgdGhlIHNlcnZpY2VzIGZvciB3aGljaCANCg== From lblazek <@t> digestivespecialists.com Mon Jul 14 10:07:12 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Mon Jul 14 10:00:07 2008 Subject: [Histonet] Schiff's removal Message-ID: <5A2BD13465E061429D6455C8D6B40E390B77E277@IBMB7Exchange.digestivespecialists.com> To who ever made the suggestion of using Anatech hand cleaner for Schiff's reagent to decolorize a PAS stain gets a GREAT BIG thanks! A whole rack of slides inadvertently got stained with the Alcian Blue/PAS. Anatech's hand cleaner did magic! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com P Please consider the environment before printing this e-mail. From eplurbus <@t> u.washington.edu Mon Jul 14 10:57:36 2008 From: eplurbus <@t> u.washington.edu (eplurbus@u.washington.edu) Date: Mon Jul 14 10:57:41 2008 Subject: [Histonet] NBT/BCIP staining for Alkaline Phosphatase Message-ID: Hello all, I am condicting a bone histomorphometry study that involves staining for detection of alkaline phosphatase on methymethacrylate embedded sections. I am able to get OK results with Roche brand NBT/BCIP ready-to-use tablets but I was wondering if anyone out there would recommend another specific brand of tablets that they have had good success with. Also, I am currently using an old bottle of Zymed Clearmount to mount the cover slips. I am not so pleased with the results. Can anyone recommend a good water based mountant? Thanks Alexander Dowell University of Washington eplurbus@u.washington.edu From gmartin <@t> marshallmedical.org Mon Jul 14 13:55:21 2008 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Mon Jul 14 13:55:36 2008 Subject: [Histonet] 6 to 48 hour fixation Message-ID: <6ED9D4252F278841A0593D3D788AF24C02D37AEA@mailsvr.MARSHMED.local> Is it true that you do not have to observe the minimum 6 hour fixation and the 48 hour maximum fixation time if you do HER2 by FISH. From tshrobertson <@t> yahoo.com Mon Jul 14 14:59:21 2008 From: tshrobertson <@t> yahoo.com (Teisha Robertson) Date: Mon Jul 14 14:59:26 2008 Subject: [Histonet] double staining Message-ID: <258358.21847.qm@web62503.mail.re1.yahoo.com> Good Afternoon, ? I am double lableling using mouse antibodies. My single staining works fine but when I double stain with the second primary anitbody I receive no response. I am using G-protiens if you were wondering.? Can anyone suggest a protocol? Any suggestions given would be greatly appreciated. ? Teisha From rjbuesa <@t> yahoo.com Mon Jul 14 15:46:50 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jul 14 15:46:55 2008 Subject: [Histonet] 6 to 48 hour fixation In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C02D37AEA@mailsvr.MARSHMED.local> Message-ID: <610970.27889.qm@web65712.mail.ac4.yahoo.com> False! Ren? J. --- On Mon, 7/14/08, Martin, Gary wrote: From: Martin, Gary Subject: [Histonet] 6 to 48 hour fixation To: histonet@lists.utsouthwestern.edu Date: Monday, July 14, 2008, 2:55 PM Is it true that you do not have to observe the minimum 6 hour fixation and the 48 hour maximum fixation time if you do HER2 by FISH. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Mon Jul 14 17:21:55 2008 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Mon Jul 14 17:22:01 2008 Subject: [Histonet] double staining In-Reply-To: <258358.21847.qm@web62503.mail.re1.yahoo.com> Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835C2C@LSRIEXCH1.lsmaster.lifespan.org> Have you tried doing the two antibodies in reverse order? Sometimes the two antigens are configured such that the binding of antibodies to one blocks the access of antibodies to the other. Picture it as one antigen protruding farther from the cell surface than the other. If you stain the "taller" antigen first, it can form a protective shield, so to speak, that prevents antibodies from penetrating to the underlying "shorter" antigen. But, if you stain the "shorter" antigen first, then the "taller" one may still protrude far enough for its anitibody to find it. From gayle.callis <@t> bresnan.net Mon Jul 14 17:47:20 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Mon Jul 14 17:47:18 2008 Subject: [Histonet] double staining References: <4EBFF65383B74D49995298C4976D1D5E03835C2C@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <001401c8e603$93200a20$6501a8c0@DHXTS541> The shielding can be done by chromogens too, Chris van der Loos teaches this in his multiple staining course. He always develops the Alk phos first, then the HRP. Hopefully he is looking in on this messaging too. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT ----- Original Message ----- From: "Monfils, Paul" To: Sent: Monday, July 14, 2008 4:21 PM Subject: RE: [Histonet] double staining Have you tried doing the two antibodies in reverse order? Sometimes the two antigens are configured such that the binding of antibodies to one blocks the access of antibodies to the other. Picture it as one antigen protruding farther from the cell surface than the other. If you stain the "taller" antigen first, it can form a protective shield, so to speak, that prevents antibodies from penetrating to the underlying "shorter" antigen. But, if you stain the "shorter" antigen first, then the "taller" one may still protrude far enough for its anitibody to find it. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Mon Jul 14 19:23:52 2008 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Mon Jul 14 19:26:35 2008 Subject: [Histonet] double staining - shielding from X-gal?? Message-ID: <179080534-1216081588-cardhu_decombobulator_blackberry.rim.net-319533194-@bxe157.bisx.prod.on.blackberry> Not to hijack the thread but does anyone know about possible shielding from X-gal staining?? -----Original Message----- From: anh2006@med.cornell.edu Date: Mon, 14 Jul 2008 23:46:01 To: Subject: double staining - shielding from X-gal?? Not to hijack the thread but does anyone know about possible shielding from X-gal staining?? ------Original Message------ From: Gayle Callis Sender: histonet-bounces@lists.utsouthwestern.edu To: Monfils, Paul To: histonet@lists.utsouthwestern.edu Sent: Jul 14, 2008 6:47 PM Subject: Re: [Histonet] double staining The shielding can be done by chromogens too, Chris van der Loos teaches this in his multiple staining course. He always develops the Alk phos first, then the HRP. Hopefully he is looking in on this messaging too. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT ----- Original Message ----- From: "Monfils, Paul" To: Sent: Monday, July 14, 2008 4:21 PM Subject: RE: [Histonet] double staining Have you tried doing the two antibodies in reverse order? Sometimes the two antigens are configured such that the binding of antibodies to one blocks the access of antibodies to the other. Picture it as one antigen protruding farther from the cell surface than the other. If you stain the "taller" antigen first, it can form a protective shield, so to speak, that prevents antibodies from penetrating to the underlying "shorter" antigen. But, if you stain the "shorter" antigen first, then the "taller" one may still protrude far enough for its anitibody to find it. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tifei <@t> foxmail.com Mon Jul 14 22:03:33 2008 From: tifei <@t> foxmail.com (tf) Date: Mon Jul 14 22:04:22 2008 Subject: [Histonet] Reply double immunostaining on same species, mouse References: <000e01c8e53c$5dfc8d90$6501a8c0@DHXTS541>, <200807141005554294023@foxmail.com>, <000f01c8e605$d30e16c0$6501a8c0@DHXTS541> Message-ID: <200807151103280794156@foxmail.com> RGVhciBHYXlsZToNCg0KVGhlIHJhdHMgYXJlIHNhY3JpZmljZWQgYW5kIHRyYW5zY2FyZGlhbGx5 IHBlcmZ1c2VkIHdpdGggc2FsaW5lIGZvbGxvd2VkIHdpdGggNCUgUEZBIChwSCA3LjQpLiBUaGVu 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ciBkeWVzLiAgR2F5bGUgTS4gQ2FsbGlzSFRML0hUL01UKEFTQ1ApQm96ZW1hbiBNVCBZb3UgY2Fu IHN0aWxsIGhhdmUgaXNzdWVzIHdpdGggdGhlIHNlY29uZCByZWQgZGV0ZWN0aW9uIHRyeWluZyB0 byBzdGljayB0byB0aGUgZmlyc3QgQUIgdW5sZXNzIHlvdSBkbyBzb21lIHZlcnkgYWdncmVzc2l2 ZSBibG9ja2luZy4NClBhdHN5DQotLS0tLU9yaWdpbmFsIE1lc3NhZ2UtLS0tLQ0KU2VudDogU2F0 dXJkYXksIEp1bHkgMTIsIDIwMDggOTo1OCBBTQ0KVG86IGhpc3RvbmV0DQpTdWJqZWN0OiBbSGlz dG9uZXRdIERvdWJsZSBpbW11bm9zdGFpbmluZyB3aXRoIHR3byBzYW1lIHNvdXJjZSAxc3QgQW50 aWJvZHkNCkhpIGFsbA0KSnVzdCB3b25kZXIgYW55IG9uZSBvZiB5b3UgdHJpZWQgdG8gcGVyZm9y bSBJSEMgdXNpbmcgdHdvIHByaW1hcnkgYW50aWJvZGllcyBmcm9tIHNhbWUgc291cmNlLCBtaWNl LCBmb3IgZXhhbXBsZT8NClRoZSBwcm9jZXNzIGlzIHRoYXQgDQpNaWNlIGFudGktQSAoMXN0IElI QyktPnNlY29uZGFyeSBhbnRpYm9keSBsYWJlbGVkIHdpdGggR1JFRU4NCmZsdXJvc2NlbmNlLT53 YXNoDQotPiBNaWNlIGFudGktQiAoMm5kIElIQyktPiBSZWQgZmx1cm9zY2VuY2UNCnRoZW4gdGhl cmUncyBubyBjcm9zcyByZWFjdGlvbi4NCnRoeC4NCjIwMDgtMDctMTIgDQp0ZiANCg== From yvan_lindekens <@t> yahoo.com Tue Jul 15 06:08:56 2008 From: yvan_lindekens <@t> yahoo.com (yvan lindekens) Date: Tue Jul 15 06:08:59 2008 Subject: [Histonet] Looking for user and/or repair manual for Leitz 1300 large base sledge microtome... Message-ID: <38541.13197.qm@web30905.mail.mud.yahoo.com> Any antique dealers here? :-))) If someone would even have a repair manual, I would be extremely gratefull... Of course I'm willing to pay for those. Thanks very much in advance! Y. From akbitting <@t> geisinger.edu Tue Jul 15 07:15:30 2008 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Tue Jul 15 07:15:42 2008 Subject: [Histonet] Androgen Receptor Message-ID: <487C5CA2.2B7F.00C9.0@geisinger.edu> If anyone is successfully running Dakos Androgen Receptor antibody on their BenchmarkXT, would they mind sharing their protocol? I know tissue varies site to site, bit it gives me somewhere to start. Thanks. IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From akbitting <@t> geisinger.edu Tue Jul 15 07:18:07 2008 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Tue Jul 15 07:18:20 2008 Subject: [Histonet] Androgen Receptor Message-ID: <487C5D3F.2B7F.00C9.0@geisinger.edu> If anyone is successfully running Dakos Androgen Receptor antibody on their BenchmarkXT, would they mind sharing their protocol? I know tissue varies site to site, bit it gives me somewhere to start. Thanks. IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From akbitting <@t> geisinger.edu Tue Jul 15 07:21:12 2008 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Tue Jul 15 07:21:22 2008 Subject: [Histonet] Androgen receptor (DAKO) Message-ID: <487C5DF8.2B7F.00C9.0@geisinger.edu> If anyone is successfully running Dakos Androgen Receptor antibody on their BenchmarkXT, would they mind sharing their protocol? I know tissue varies site to site, bit it gives me somewhere to start. Thanks. IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From S.MacPherson <@t> hrsu.mrc.ac.uk Tue Jul 15 08:32:59 2008 From: S.MacPherson <@t> hrsu.mrc.ac.uk (Sheila MacPherson) Date: Tue Jul 15 08:33:16 2008 Subject: [Histonet] double immunostaining on same species [Scanned] In-Reply-To: References: Message-ID: <86F334797DC6524A99AD9DD8F23A8B50111A42@mailserv.hrsu.mrc.ac.uk> There are several option for doing double or triple colocalisations using the same species of primary antibodies on any species. Option 1 Combine a sensitive detection system e.g tyramide with a less sensitive detection system e.g indirect (using fluorescently labeled secondary). The theory here is that you can use your first primary Ab using a tyramide detection at a dilution beyond the detection limits of the second detection indirect). A normal serum block between the detections can prove effective. Option 2 Use monovalent secondary antibodies for your detection e.g Goat anti Mouse Fab Biotinylated followed by a fluorescent streptavidin. Basically you repeat this detection sequentially for each primary using a different fluorophore. It can help to do a unconjugated fab block in between detections. In our hand this works best with monoclonal primaries but can have success with polyclonals as well but it is a bit trickier. Rule here is to use the same monovalent secondary for each detection. Because of the monovalent nature of the secondary there is little/no cross reaction. Unfortunately the monovalent secondaries on the market are polyclonal there for it is best to use the same secondary reagent as a different (polyclonal monovalent secondary) may recognize different epitopes on your primary. Option 3 Use a tyramide detection for each of your primaries and perform a heat induced antigen retrieval step in between. This is pretty bomb proof. It can be used for double any species!!!!!!!!! providing your epitope is not destroyed by the HIER, in our hands generally HIER will improve antigenicity rather than be detrimental although this is not always the case, you just have to try it and see. To our knowledge this will only work with tyramide detections as the HIER seems to strip away any tissue bound antibodies, hence why it gives very clean co-localisations with no possibility of cross reactions. The HRP catalysed deposition of fluorescently labeled tyramide around the site of the HRP means that the fluorescent tyramide binds to the tissue around the site of the HRP labeled secondary and is not attached to the secondary antibody. For some reason the HIER does not affect the tyramide tissue binding, which is fortunate but it does destroy/denatured/strip away the antibodies and any possibility of cross reactions associated with them. Of course if one of your antibodies is destroyed by retrieval then do this detection first without retrieval! If retrieval destroys both your epitopes you are really unlucky and I wouldn't waste your money on a lottery ticket this week. Option 4 It does not have to be fluorescent, colourometric (DAB and fast blue e.g) can give pretty good results especially if the epitopes are not colocalised to the same cellular structure so nuclear and cytoplasmic or different cell types within the same tissue give good results. The colours do get a bit muddy if things colocalise to the same structure though. Again do a HIER step between detections to remove any possibility of cross reactions............sorted! Finally if you are being daring and imaginative you can try a combination of the above methods BUT you must do appropriate controls to prove that you truly are seeing a real colocalisation as opposed to a detection cross reaction. Id love to take credit for thinking up these cunning ideas however this is no the case and a search of the literature should pull up the relevant publications. Mike Millar, Sheila MacPherson, Arantza Esnal and Nancy Evans MRC Human Reproductive Sciences Unit, Edinburgh, Scotland UK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: 14 July 2008 18:26 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 56, Issue 17 [Scanned] Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Reticular stain (Dunfee, LuWanda R) 2. Re: Reticular stain (Rene J Buesa) 3. Reply double immunostaining on same species, mouse (Gayle Callis) 4. Re: Double immunostaining with two same source 1st Antibody (tf) 5. Re: Reply double immunostaining on same species, mouse (tf) 6. Re: Re: [Histonet] Double immunostaining with two same source 1stAntibody (tf) 7. Re: Re: RE: [Histonet] Anterograde tracing (tf) 8. RE: freezing artefact holes on edge of cortex in mouse brain sections (Aine Behan) 9. Re: RE: freezing artefact holes on edge of cortex in mousebrain sections (tf) 10. Re: RE: [Histonet] Double immunostaining with two same source 1st Antibody (tf) 11. Schiff's removal (Blazek, Linda) 12. NBT/BCIP staining for Alkaline Phosphatase (eplurbus@u.washington.edu) ---------------------------------------------------------------------- Message: 1 Date: Sun, 13 Jul 2008 13:00:39 -0700 From: "Dunfee, LuWanda R" Subject: [Histonet] Reticular stain To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hello, I am working on the reticular stain that we are currently using. We are using a modified version of the Gordon and Sweet's stain. It is not selective enough, and it is picking up other collagen fibers. I was wondering if anyone had any suggestions. Thank You, LuWanda Seattle Cancer Care Alliance This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 288-6266, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. ------------------------------ Message: 2 Date: Sun, 13 Jul 2008 13:35:37 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Reticular stain To: histonet@lists.utsouthwestern.edu, "Dunfee, LuWanda R" Message-ID: <48288.32832.qm@web65702.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Try Gomori's method. Ren? J. --- On Sun, 7/13/08, Dunfee, LuWanda R wrote: From: Dunfee, LuWanda R Subject: [Histonet] Reticular stain To: histonet@lists.utsouthwestern.edu Date: Sunday, July 13, 2008, 4:00 PM Hello, I am working on the reticular stain that we are currently using. We are using a modified version of the Gordon and Sweet's stain. It is not selective enough, and it is picking up other collagen fibers. I was wondering if anyone had any suggestions. Thank You, LuWanda Seattle Cancer Care Alliance This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 288-6266, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Sun, 13 Jul 2008 17:01:21 -0600 From: "Gayle Callis" Subject: [Histonet] Reply double immunostaining on same species, mouse To: "Histonet" Message-ID: <000e01c8e53c$5dfc8d90$6501a8c0@DHXTS541> Content-Type: text/plain; charset="iso-8859-1" We do double and triple immunfluorescence staining on mouse tissues with all antibodies raised in same host species only on mouse tissues, rat antiMouse. It takes some careful blocking and organization, but it works beautifully. Please contact me and I will discuss the details on how to set up the staining, but supply some details on what you want to see as green or red fluorescence. This is only done on frozen sections and NOT on formalin fixed paraffin embedded tissue to eliminate autofluorescence. There are several ways to set this up, and we do use some biotinylated primary antibodies along with Molecular Probes Streptavidin Alexa fluor dyes. Gayle M. CallisHTL/HT/MT(ASCP)Bozeman MT You can still have issues with the second red detection trying to stick to the first AB unless you do some very aggressive blocking. Patsy -----Original Message----- Sent: Saturday, July 12, 2008 9:58 AM To: histonet Subject: [Histonet] Double immunostaining with two same source 1st Antibody Hi all Just wonder any one of you tried to perform IHC using two primary antibodies from same source, mice, for example? The process is that Mice anti-A (1st IHC)->secondary antibody labeled with GREEN fluroscence->wash -> Mice anti-B (2nd IHC)-> Red fluroscence then there's no cross reaction. thx. 2008-07-12 tf _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- << Previous Message | Next Message >> ------------------------------ Message: 4 Date: Mon, 14 Jul 2008 10:02:23 +0800 From: "tf" Subject: Re: [Histonet] Double immunostaining with two same source 1st Antibody To: "Neil Fournier" , "histonet" Message-ID: <200807141002185117220@foxmail.com> Content-Type: text/plain; charset="gb2312" Some one suggested a "citrate buffer" treatment before 2nd IHC. However he als suggested be careful about losing anti-gen in this step. Actually I used this step for BrdU staining pretreatment before adding HCl to broken DNA helix (brdu can be taken by cell and synthsized into DNA). It seems that citrate buffer has more function than simply the antigen retrieval. Can anyone comment on this further? 2008-07-14 tf ???????? Neil Fournier ?????????? 2008-07-14 03:26:20 ???????? tifei@foxmail.com ?????? ?????? [Histonet] Double immunostaining with two same source 1st Antibody I am extremely interested in the suggestions you receive. I would like to do a similar procedure. If possible, would you be able forward them to me. Much appreciated, Neil Neil M. Fournier Department of Psychology University of Saskatchewan 9 Campus Drive Saskatoon, SK, Canada S7N 5A5 Phone: 306-966-4024 Fax: 306-966-6630 ------------------------------ Message: 5 Date: Mon, 14 Jul 2008 10:06:00 +0800 From: "tf" Subject: Re: [Histonet] Reply double immunostaining on same species, mouse To: "Gayle Callis" , "Histonet" Message-ID: <200807141005554294023@foxmail.com> Content-Type: text/plain; charset="gb2312" That's cool and please kindly let me know the detail. Yes we used PFA to fix tissue and we have both microtome / cryostat to make sections. the cryostat sections are embeded with O.C.T. We have several mouse monoclonal antibodies, and I will use only Alexa fluroscence conjugated 2nd antibody rather DAB staining. Thanks a lot. 2008-07-14 tf ???????? Gayle Callis ?????????? 2008-07-14 07:05:57 ???????? Histonet ?????? ?????? [Histonet] Reply double immunostaining on same species, mouse We do double and triple immunfluorescence staining on mouse tissues with all antibodies raised in same host species only on mouse tissues, rat antiMouse. It takes some careful blocking and organization, but it works beautifully. Please contact me and I will discuss the details on how to set up the staining, but supply some details on what you want to see as green or red fluorescence. This is only done on frozen sections and NOT on formalin fixed paraffin embedded tissue to eliminate autofluorescence. There are several ways to set this up, and we do use some biotinylated primary antibodies along with Molecular Probes Streptavidin Alexa fluor dyes. Gayle M. CallisHTL/HT/MT(ASCP)Bozeman MT You can still have issues with the second red detection trying to stick to the first AB unless you do some very aggressive blocking. Patsy -----Original Message----- Sent: Saturday, July 12, 2008 9:58 AM To: histonet Subject: [Histonet] Double immunostaining with two same source 1st Antibody Hi all Just wonder any one of you tried to perform IHC using two primary antibodies from same source, mice, for example? The process is that Mice anti-A (1st IHC)->secondary antibody labeled with GREEN fluroscence->wash -> Mice anti-B (2nd IHC)-> Red fluroscence then there's no cross reaction. thx. 2008-07-12 tf _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- << Previous Message | Next Message >> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu ------------------------------ Message: 6 Date: Mon, 14 Jul 2008 10:08:38 +0800 From: "tf" Subject: Re: Re: [Histonet] Double immunostaining with two same source 1stAntibody To: "Igor Nasonkin" , "histonet" Message-ID: <200807141008330513746@foxmail.com> Content-Type: text/plain; charset="gb2312" Thanks very much Igor. I'd like to share the information to other people who are interested in this. "www.jacksonimmuno.com Check out their web site-technical pages-staining with 2 Abs from same sources. Their protocols are good and they do work Luck igor" 2008-07-14 tf ???????? Igor Nasonkin ?????????? 2008-07-14 10:06:55 ???????? tifei@foxmail.com ?????? ?????? Re: [Histonet] Double immunostaining with two same source 1stAntibody Www.jacksonimmuno.com Check out their web site-technical pages-staining with 2 Abs from same sources. Their protocols are good and they do work Luck igor On 7/13/08 10:02 PM, "tf" wrote: > Some one suggested a "citrate buffer" treatment before 2nd IHC. > However he als suggested be careful about losing anti-gen in this step. > > Actually I used this step for BrdU staining pretreatment before adding HCl to > broken DNA helix (brdu can be taken by cell and synthsized into DNA). > It seems that citrate buffer has more function than simply the antigen > retrieval. > > Can anyone comment on this further? > > > 2008-07-14 > > > > tf > > > > ???????? Neil Fournier > ?????????? 2008-07-14 03:26:20 > ???????? tifei@foxmail.com > ?????? > ?????? [Histonet] Double immunostaining with two same source 1st Antibody > > I am extremely interested in the suggestions you receive. I would like to do a > similar procedure. If possible, would you be able forward them to me. > > Much appreciated, > > Neil > > Neil M. Fournier > Department of Psychology > University of Saskatchewan > 9 Campus Drive > Saskatoon, SK, Canada > S7N 5A5 > > Phone: 306-966-4024 > Fax: 306-966-6630 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Mon, 14 Jul 2008 10:11:10 +0800 From: "tf" Subject: Re: Re: RE: [Histonet] Anterograde tracing To: "histonet" Message-ID: <200807141011050118208@foxmail.com> Content-Type: text/plain; charset="gb2312" Hi all again. Just wonder anyone tried WGA-HRP? it seems that WGA-hRP has a good diffusion ability,, making it ideal for local pressure injection (quite simple method), which PHA-L does bad. and you can detect it well. anyone have worked with this? thx 2008-07-14 tf ???????? tf ?????????? 2008-07-12 00:50:29 ???????? Rachel, Rivka (NIH/NEI) [E]; histonet ?????? ?????? Re: RE: [Histonet] Anterograde tracing Dear Rachel: Surely I have considered that. I am actually working on regeneration. Also, dyes are versatile and go everywhere as long as membrane permits. It does not have the restricted labeling of of one batch of projection and may diffuse a lot when tracing the transection site. Another problem you have mentioned is that DiI does not diffuse well in adult tissue, and thus used more in developmental studies. thanks a lot 2008-07-12 tf ???????? Rachel, Rivka (NIH/NEI) [E] ?????????? 2008-07-12 00:00:49 ???????? tifei@foxmail.com; histonet ?????? ?????? RE: [Histonet] Anterograde tracing What type of tissue are you trying to trace? If fixed, immature rodent tissue, have you considered one of the fluorescent tracers such as DiI, DiO, DiD, etc. available through Molecular Probes (Invitrogen)? DiI (red fluorescence) is the brightest. These dyes don't work well in adult tissue, however. Rivka Rivka A. Rachel, MD, PhD Staff Scientist, National Eye Institute Neurobiology-Neurodegeneration and Repair Laboratory Tel: 301 443-4906 -----Original Message----- From: tf [mailto:tifei@foxmail.com] Sent: Fri 7/11/2008 11:41 AM To: histonet Subject: [Histonet] Anterograde tracing I just want to discuss the best anterograde tracer. BDA, PHA-L, CTB-FITC, CTB-Rhodamine, WGA-HRP, HRP, and viral vectors all could be anterograde tracer. However, BDA is diffusible like all fluroscent dyes, does not have the necessary direction. CTB-conjugated sereis have limited diffusion ability when injected with pressure, just like PHA-L, thus are not good in labeling a larger group of neurons when single rather multiple injections is required. WGA-HRP & HRP both antero & retrogradely transported, HRP & virus even transynaptically. So, any one has other ideas? Such as shorten the period after injection to reduce the diffusion, or using immunodetection to visualize the CTB-FITC, for example? 2008-07-11 tf _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Mon, 14 Jul 2008 14:52:26 +0100 From: "Aine Behan" Subject: [Histonet] RE: freezing artefact holes on edge of cortex in mouse brain sections To: Message-ID: <3DBACD0C44A1CF429431C0C6DC3E81690324DA08@crc_exchange.rcsi-internal.ie> Content-Type: text/plain; charset="iso-8859-1" Hi all, I was looking for info on troubleshooting what I think is freezing artefact holes (FA) in our tissue sections. It is quite specific to the edge of the brain sections predominantly around the most superficial layers of the cortex and most obvious in the most lateral aspects of the cortex in coronal sections. Brains were fixed in 4% PFA following cervical dislocation and dissection. They were fixed in graded sucrose concentrations of 10%, 20% and then 30% over 3 days followed by rapid freezing in isopentane and dry ice. These brains are stored at -80 until cryostating coronal sections (10 microns thick). The deeper brain structures are perfectly intact and free from FA holes. Has anyone observed this before and do you think this is FA or something else? I can send on a H&E pic if it proves helpful. It might be a bizarre question but could there be anything to do with the cryostat that could be causing this!?! Regards, ?ine ?ine Behan, PhD Department of Psychiatry, Royal College of Surgeons In Ireland, Smurfit Building, Beaumont Hospital, Dublin 9. Ph: +353-1-809-3857/3798 Fx: +353-1-809-3741 ------------------------------ Message: 9 Date: Mon, 14 Jul 2008 22:36:46 +0800 From: "tf" Subject: Re: [Histonet] RE: freezing artefact holes on edge of cortex in mousebrain sections To: "Aine Behan" , "histonet@lists.utsouthwestern.ed" Message-ID: <200807142236417873896@foxmail.com> Content-Type: text/plain; charset="gb2312" Dear Aine: Yes that's a common problem for cryostat sections. Different people in my lab has quite diverse response to this. Some suggested that you can leave the brain in O.C.T before put them in to freezer for 3-6 hours, and the O.C.T will "enter" the brain tissue. Then no "cheese". Some other people tried the other half of the brain on microtome, to see if this helps. To our surprise, these are no such hollow cavities in microtome-made sections. Thus possibly it's related to O.C.T embedding rather bad dehydration/fixation/post-fixation. Cheers, Ti Fei. 2008-07-14 Department of ANATOMY The UNIVERSITY OF HONG KONG LI KAI SHING FACULTY OF MEDICINE 21 Sassoon Road, Pok Fu Lam Hong Kong ???????? Aine Behan ?????????? 2008-07-14 21:58:34 ???????? histonet@lists.utsouthwestern.edu ?????? ?????? [Histonet] RE: freezing artefact holes on edge of cortex in mousebrain sections Hi all, I was looking for info on troubleshooting what I think is freezing artefact holes (FA) in our tissue sections. It is quite specific to the edge of the brain sections predominantly around the most superficial layers of the cortex and most obvious in the most lateral aspects of the cortex in coronal sections. Brains were fixed in 4% PFA following cervical dislocation and dissection. They were fixed in graded sucrose concentrations of 10%, 20% and then 30% over 3 days followed by rapid freezing in isopentane and dry ice. These brains are stored at -80 until cryostating coronal sections (10 microns thick). The deeper brain structures are perfectly intact and free from FA holes. Has anyone observed this before and do you think this is FA or something else? I can send on a H&E pic if it proves helpful. It might be a bizarre question but could there be anything to do with the cryostat that could be causing this!?! Regards, ?ine ?ine Behan, PhD Department of Psychiatry, Royal College of Surgeons In Ireland, Smurfit Building, Beaumont Hospital, Dublin 9. Ph: +353-1-809-3857/3798 Fx: +353-1-809-3741 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu ------------------------------ Message: 10 Date: Mon, 14 Jul 2008 22:40:16 +0800 From: "tf" Subject: Re: RE: [Histonet] Double immunostaining with two same source 1st Antibody To: "Anatoli Gleiberman" Cc: histonet Message-ID: <200807142240108583751@foxmail.com> Content-Type: text/plain; charset="gb2312" Dear Anatoli: Thanks very much for your suggestions. Yes I am using Abcam rat monoclonal Brdu antibody, and I also have tried mouse monoclonal. SOme people in my lab suggested that without HCl, you may get better DAPI co-staining after BrdU staining, however sometimes you can not get BrdU staining... We dont know why. Citrate buffer might be able to break some DNA helix during heating process (30 min with 95 degree). But I was just following the protocol. Next time I will try it. - - cheers. 2008-07-14 tf ???????? Anatoli Gleiberman ?????????? 2008-07-14 21:57:39 ???????? tifei@foxmail.com ?????? ?????? RE: [Histonet] Double immunostaining with two same source 1st Antibody For BrdU staining boiling in citrate buffer is good enough to denature DNA - you don't need to treat sections with HCl after that. I did it on both paraffin and cryo sections with the same good results. Another suggestion - for BrdU staining use rat monoclonal against BrdU from Apcam - they are much more stronger and cleaner than any mouse monoclonal I have used so far. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman@cbiolabs -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of tf Sent: Sunday, July 13, 2008 10:02 PM To: Neil Fournier; histonet Subject: Re: [Histonet] Double immunostaining with two same source 1st Antibody Some one suggested a "citrate buffer" treatment before 2nd IHC. However he als suggested be careful about losing anti-gen in this step. Actually I used this step for BrdU staining pretreatment before adding HCl to broken DNA helix (brdu can be taken by cell and synthsized into DNA). It seems that citrate buffer has more function than simply the antigen retrieval. Can anyone comment on this further? 2008-07-14 tf ???????? Neil Fournier ?????????? 2008-07-14 03:26:20 ???????? tifei@foxmail.com ?????? ?????? [Histonet] Double immunostaining with two same source 1st Antibody I am extremely interested in the suggestions you receive. I would like to do a similar procedure. If possible, would you be able forward them to me. Much appreciated, Neil Neil M. Fournier Department of Psychology University of Saskatchewan 9 Campus Drive Saskatoon, SK, Canada S7N 5A5 Phone: 306-966-4024 Fax: 306-966-6630 This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which ------------------------------ Message: 11 Date: Mon, 14 Jul 2008 11:07:12 -0400 From: "Blazek, Linda" Subject: [Histonet] Schiff's removal To: "histonet@pathology.swmed.edu" Message-ID: <5A2BD13465E061429D6455C8D6B40E390B77E277@IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" To who ever made the suggestion of using Anatech hand cleaner for Schiff's reagent to decolorize a PAS stain gets a GREAT BIG thanks! A whole rack of slides inadvertently got stained with the Alcian Blue/PAS. Anatech's hand cleaner did magic! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com P Please consider the environment before printing this e-mail. ------------------------------ Message: 12 Date: Mon, 14 Jul 2008 08:57:36 -0700 (PDT) From: eplurbus@u.washington.edu Subject: [Histonet] NBT/BCIP staining for Alkaline Phosphatase To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed Hello all, I am condicting a bone histomorphometry study that involves staining for detection of alkaline phosphatase on methymethacrylate embedded sections. I am able to get OK results with Roche brand NBT/BCIP ready-to-use tablets but I was wondering if anyone out there would recommend another specific brand of tablets that they have had good success with. Also, I am currently using an old bottle of Zymed Clearmount to mount the cover slips. I am not so pleased with the results. Can anyone recommend a good water based mountant? Thanks Alexander Dowell University of Washington eplurbus@u.washington.edu ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 56, Issue 17 **************************************** From stephanie.d.rivera <@t> gsk.com Tue Jul 15 08:44:55 2008 From: stephanie.d.rivera <@t> gsk.com (stephanie.d.rivera@gsk.com) Date: Tue Jul 15 08:45:53 2008 Subject: [Histonet] Androgen Receptor In-Reply-To: <487C5CA2.2B7F.00C9.0@geisinger.edu> Message-ID: I have not used DAKO Androgen Antibody but I have tried the following: I have run Affinity BioReagents using Ventana XT DAB MAP KIT (1:50, 1:75, 1:100, 1:200) 60 minutes Primary, CC1 mild Ultra MAP (1:500, 1:1000) 32 minutes, CC1 mild Control tissue RAT Repro (Prostate, Seminal Vesicles) ALSO Had success with Santa Cruz Polyclonal Rabbit Androgen(N20 clone) SC-816 Used AR Nuclear Decloaker PH9.0 in pressur cooker Incubate overnight 4 degrees refrigerate Rabbit envision-hrp(DAKO) for detection Run @1ug/ml or 1:200 Control: Rat male repro(prostate) nuclear staining observed in epithelial cells of prostate. Stephanie D. Rivera Safety Assessment Department GlaxoSmithKline 709 Swedeland RD King of Prussia, PA 19406 phone: 610-270-7340 fax: 610-270-7202 From tp2 <@t> medicine.wisc.edu Tue Jul 15 09:24:33 2008 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Tue Jul 15 09:25:07 2008 Subject: [Histonet] Androgen Receptor Message-ID: <487C6CD1020000DF0000CBE8@gwutil.medicine.wisc.edu> I had good results with Biocare Medical's androgen receptor antibody. From Jackie.O'Connor <@t> abbott.com Tue Jul 15 09:57:47 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue Jul 15 09:58:05 2008 Subject: [Histonet] Beecher ATA27 Problem Resolved In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C02D37AEA@mailsvr.MARSHMED.local> Message-ID: Thanks to those who responded to my SOS for help with the arrayer in a friend's lab. However, the problem was quickly resolved by the technical staff at Beecher after a round of phone tag - Dan was actually trying to reach the lab on one line while they were trying to reach him on another! Bottom line is Dan had the equipment up and running quickly - thanks Dan! It turned out to be less of an issue than they thought - always remember - don't panic! The unit is back to producing beautiful arrays! Jackie O' From jmjohnson34 <@t> hotmail.com Tue Jul 15 10:43:24 2008 From: jmjohnson34 <@t> hotmail.com (Jennifer Johnson) Date: Tue Jul 15 10:43:29 2008 Subject: [Histonet] FNA procedure Message-ID: Does anyone have an FNA procedure that they are willing to share. Until now, we were not required to be present for smears. Thanks, Jennifer _________________________________________________________________ Use video conversation to talk face-to-face with Windows Live Messenger. http://www.windowslive.com/messenger/connect_your_way.html?ocid=TXT_TAGLM_WL_Refresh_messenger_video_072008 From pjfnefro <@t> duke.edu Tue Jul 15 11:50:04 2008 From: pjfnefro <@t> duke.edu (Pat Flannery) Date: Tue Jul 15 11:50:09 2008 Subject: [Histonet] Villanueva Stain? Message-ID: <295DA3BC-EB05-489E-874C-200CFC56F196@duke.edu> We use Villanueva Mineralized Bone Stain for research specimens and when our lab moved into a new building, our supply was lost (probably thrown out by someone "cleaning up" for me). Where do you folks get this stain? I used to order it from Dr. Villanueva but I've lost touch with him and he hasn't answered my emails (perhaps the address I have for him is too old). Thanks for any help. -Pat Flannery Duke Med From Robert.Schoonhoven <@t> mpiresearch.com Tue Jul 15 11:58:02 2008 From: Robert.Schoonhoven <@t> mpiresearch.com (Robert Schoonhoven) Date: Tue Jul 15 11:58:08 2008 Subject: [Histonet] Villanueva Stain? In-Reply-To: <295DA3BC-EB05-489E-874C-200CFC56F196@duke.edu> Message-ID: Tony has long since retired but doing well as I saw him at the last NSH meeting. His stain can be purchased through Polysciences. Bob Robert Schoonhoven BS, HT, HTL (ASCP) Scientist, Pathology MPI Research 54943 North Main Street Mattawan, MI 49071-9399 E-Mail: robert.schoonhoven@mpiresearch.com Office 269.668.3336 X-1768 Lab. 269.668.3336 X-2009 Cell 269.615.0576 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat Flannery Sent: Tuesday, July 15, 2008 12:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Villanueva Stain? We use Villanueva Mineralized Bone Stain for research specimens and when our lab moved into a new building, our supply was lost (probably thrown out by someone "cleaning up" for me). Where do you folks get this stain? I used to order it from Dr. Villanueva but I've lost touch with him and he hasn't answered my emails (perhaps the address I have for him is too old). Thanks for any help. -Pat Flannery Duke Med _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication, including attachments, is for the exclusive use of addressee and may contain proprietary, confidential and/or privileged information. If you are not the intended recipient, any use, copying, disclosure, dissemination or distribution is strictly prohibited. If you are not the intended recipient, please notify the sender immediately by return e-mail, delete this communication and destroy all copies. From pjfnefro <@t> duke.edu Tue Jul 15 12:09:45 2008 From: pjfnefro <@t> duke.edu (Pat Flannery) Date: Tue Jul 15 12:09:50 2008 Subject: [Histonet] Villanueva Stain? In-Reply-To: References: Message-ID: <0B4047F3-F6D8-453F-90C0-C6FFE39C2DC2@duke.edu> Thank you, Bob. I can remember getting it from Dr. Villanueva, then from one of his assistants, and then the trail dried up. I'll get in touch with Polysciences (Duke prefers working with a vendor anyway - they're pretty picky about sending checks to people). -Pat On Jul 15, 2008, at 12:58 PM, Robert Schoonhoven wrote: > Tony has long since retired but doing well as I saw him at the last > NSH > meeting. His stain can be purchased through Polysciences. > > Bob > > Robert Schoonhoven BS, HT, HTL (ASCP) > Scientist, Pathology > MPI Research > 54943 North Main Street > Mattawan, MI 49071-9399 > > E-Mail: robert.schoonhoven@mpiresearch.com > Office 269.668.3336 X-1768 > Lab. 269.668.3336 X-2009 > Cell 269.615.0576 > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat > Flannery > Sent: Tuesday, July 15, 2008 12:50 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Villanueva Stain? > > We use Villanueva Mineralized Bone Stain for research specimens and > when our lab moved into a new building, our supply was lost (probably > thrown out by someone "cleaning up" for me). > > Where do you folks get this stain? I used to order it from Dr. > Villanueva but I've lost touch with him and he hasn't answered my > emails (perhaps the address I have for him is too old). > > Thanks for any help. > > -Pat Flannery > Duke Med > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This communication, including attachments, is for the exclusive use > of addressee and > may contain proprietary, confidential and/or privileged information. > If you are not > the intended recipient, any use, copying, disclosure, dissemination > or distribution > is strictly prohibited. If you are not the intended recipient, > please notify the sender > immediately by return e-mail, delete this communication and destroy > all copies. > From Janet.Bonner <@t> FLHOSP.ORG Tue Jul 15 12:08:45 2008 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Tue Jul 15 12:12:05 2008 Subject: [Histonet] FNA procedure References: Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F270A@fhosxchmb006.ADVENTISTCORP.NET> Jennifer, The best thing to do in this instance is to visit a Cytology Department (who usually does these at the procedure). I could send you a write up, but there are so many hands-on things to know, it would be difficult to describe them (like making the smear). Janet Janet L. Bonner, HTL (ASCP) Pathology Laboratory Florida Hospital Winter Park janet.bonner@FLHOSP.org 407-646-7559 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jennifer Johnson Sent: Tue 7/15/2008 11:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FNA procedure Does anyone have an FNA procedure that they are willing to share. Until now, we were not required to be present for smears. Thanks, Jennifer _________________________________________________________________ Use video conversation to talk face-to-face with Windows Live Messenger. http://www.windowslive.com/messenger/connect_your_way.html?ocid=TXT_TAGLM_WL_Refresh_messenger_video_072008_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From Pkarlisch <@t> hmc.psu.edu Tue Jul 15 12:13:37 2008 From: Pkarlisch <@t> hmc.psu.edu (Patricia Karlisch) Date: Tue Jul 15 12:14:01 2008 Subject: [Histonet] Cocktail TTF-1 and LCA Message-ID: <487CA281.07F7.008C.0@hmc.psu.edu> Histonetters, Does anyone have a cocktail for TTF-1 and LCA antibodies. Have your tried to develop this cocktail yourself? I would appreciate any information our there. We prefer not to double stain. One is a nuclear stain and the other is a cytoplasmic stain and should be detected by one chromogen. Thank you in advance, Pat Karlisch Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. From karen-adamski <@t> IDEXX.com Tue Jul 15 13:00:01 2008 From: karen-adamski <@t> IDEXX.com (Adamski, Karen) Date: Tue Jul 15 13:00:30 2008 Subject: [Histonet] Senior Histology Technician Needed Message-ID: Hello! Idexx Laboratories is looking for a Histology Technician to join our team in Denver CO. If you are interested, please review the job description below. Contact information may be found at the bottom of this email. Thank you! Seeking a Senior Laboratory Technician - Histology who will be responsible for the production of quality histologic slides for microscopic examination by Veterinary Pathologists. Performs quality control duties according to the guidelines for good laboratory practices. This position is a Full Time Day position, Monday through Friday position from 6:00 am to 2:30 pm. Occasional Saturdays and Sundays. PRIMARY DUTIES AND RESPONSIBILITIES: * Extensive knowledge of the anatomy of a wide variety of species of animals * The ability to recognize abnormalities lesions. * Knowledge of the principles of tissue processing. * Manually process tissue for embedding and/or process tissue. * Ability to teach these principles and solve any and all problems that may occur. * Skills in tissue orientation in paraffin to facilitate microtomy. * Understanding in the principles of microtomy * Understanding in the principles of frozen sections * Some knowledge of routine H&E staining * Understanding of an automatic stainer * Ability to stain manually if needed * Learn a wide variety of special stain procedures and problem solve the usual difficulties. * Skills in reading and following a recipe accurately * Responsible for the maintenance, including clean up, of all equipment and workspace * Coverslip microscope slides. * Must have knowledge of and be able to use automatic coverslipper * Organizational skills to label and arrange microscope slides according to pathologists work list * Organize research slides according to histopathology worksheets delivered to the QC tech * Read, understand and follow the SOPs * Spot check filing- systems for errors * Insure supplies for filing, slides, paraffin blocks and wet tissue is always available * Perform weekly and monthly inventory * Help maintain a clean and safe work environment * Other duties as assigned. MINIMUM QUALIFICATIONS: EDUCATION: * High School diploma or equivalent * HT (ASCP) or HTL (ASCP) license required or eligible EXPERIENCE: * Minimum 5 years experience as a histotechnician in a histopathology laboratory * Experience with immunohistochemistry preferred For more information, please visit www.IDEXX.com . Karen Adamski IDEXX Laboratories Recruiter Direct (408) 440-2839 Toll Free (800) 548-6733 ext. 63643 From RSRICHMOND <@t> aol.com Tue Jul 15 13:22:05 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Tue Jul 15 13:22:11 2008 Subject: [Histonet] Re: 6 to 48 hour fixation for breast tissue Message-ID: Gary Martin asks >>Is it true that you do not have to observe the minimum 6 hour fixation and the 48 hour maximum fixation time if you do HER2 by FISH?<< This grumpy old pathologist now requires overnight fixation of all breast tissue. It isn't just regulator compliance for HER2 - what I like is the better quality of the H & E sections. Breast pathology is tough enough without crappy nuclei. The regulators have made us do what we should have been doing anyway. I'm seeing a lot of large core stereotactic biopsy specimens these days. Should these go in a bare cassette, or between blue biopsy pads? Should I be trying to cut them longitudinally before submitting them? Bob Richmond Samurai Pathologist Knoxville TN From c.m.vanderloos <@t> amc.uva.nl Tue Jul 15 13:33:33 2008 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Tue Jul 15 13:33:56 2008 Subject: [Histonet] RE: double staining Message-ID: Dear Teisha,Because you gave us only a very few details I have to speculate a bit about the cause of your failing second primary. Paul's suggestion about the second staining sequence is fully correct. I would like to add that I believe this only happens when the two epitopes of interest are really very close together. The only time we saw this actually happen was when we tried to combine CD3 and the gamma-delta Tcell receptor, at the time that it was unknown that both molecules belongs to the same complex. Perhaps more likely is that you are performing a so called 'sequential' type of double staining that starts with a complete staining procedure including HRP activity development with DAB. Because the DAB reaction product shields so effectively one can perform a second staining sequence (under ideal conditions) without any cross-reaction problems. Even with two mouse primaries. As far as I know DAB is the only enzymatic reaction product that has this 'sheltering' characteristic. Sometimes this sheltering effect also blocks the second antigenic site. Mostly when the two epitopes are very close together, but sometimes also just at random. In either case you end up with unwanted single-staining as you described. As a solution to your problem, you can try to stain the first epitope with a red alk. phos. reaction product, then perform a second HIER step for only 10 min at 98C (in a buffer that fits best with your second epitope) and perform a second staining sequence ending with a blue alk. phosp. reaction product, or whatever chromogen you like. The second HIER step removes and/or destroys your first primary antibody and detection reagents, but leaves the red alk. phosp. reaction product unchanged.Hope this helps a bitChris Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Mon, 14 Jul 2008 12:59:21 -0700 (PDT) From: Teisha Robertson Subject: [Histonet] double staining To: h n Good Afternoon, I am double lableling using mouse antibodies. My single staining works fine but when I double stain with the second primary anitbody I receive no response. I am using G-protiens if you were wondering. Can anyone suggest a protocol? Any suggestions given would be greatly appreciated. Teisha From gmartin <@t> marshallmedical.org Tue Jul 15 15:59:26 2008 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Tue Jul 15 15:59:39 2008 Subject: [Histonet] Re: 6 to 48 hour fixation for breast tissue In-Reply-To: References: Message-ID: <6ED9D4252F278841A0593D3D788AF24C02D7BE62@mailsvr.MARSHMED.local> Dr. Richmond I agree with you on the fixation of overnight ... It's better for everyone! My question is centered around the longer fixation time of more than 48 hours with breast specimens that could be subjected to HER2 testing. I am being told that if you submit a specimen that has been in 10% buffered formalin for more than the 48 hour limit ... you can submit the specimen straight to HER2 by FISH and the time rules do not apply. Have you heard this to be true or false. We also have been receiving large core stereotactic specimens. My guys are submitting them in sponges stretched out and processed without sectioning. Laying them out in the sponges seems to help avoid the spaghetti effect. Gary -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Tuesday, July 15, 2008 11:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: 6 to 48 hour fixation for breast tissue Gary Martin asks >>Is it true that you do not have to observe the minimum 6 hour fixation and the 48 hour maximum fixation time if you do HER2 by FISH?<< This grumpy old pathologist now requires overnight fixation of all breast tissue. It isn't just regulator compliance for HER2 - what I like is the better quality of the H & E sections. Breast pathology is tough enough without crappy nuclei. The regulators have made us do what we should have been doing anyway. I'm seeing a lot of large core stereotactic biopsy specimens these days. Should these go in a bare cassette, or between blue biopsy pads? Should I be trying to cut them longitudinally before submitting them? Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cforster <@t> umn.edu Tue Jul 15 16:02:25 2008 From: cforster <@t> umn.edu (Colleen Forster) Date: Tue Jul 15 16:02:27 2008 Subject: [Histonet] ICAM and VCAM Message-ID: <487D1061.4090404@umn.edu> Hello histonetters, Have any of you had success with the ICAM and VCAM IHC on mouse tissues? If so, could you share your protocol and antibody vendor. Thanks, Colleen Forster U of MN From kenneth.a.troutman <@t> Vanderbilt.Edu Tue Jul 15 17:12:02 2008 From: kenneth.a.troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Tue Jul 15 17:15:25 2008 Subject: [Histonet] Autofluorescence blocking Message-ID: <37DEF9AF72994947AF693956A59B9B660127FF22@mailbe03.mc.vanderbilt.edu> Dear Histonetters, I read awhile back about a reagent that you can use to block autofluorescence in tissue, but I cannot find the histonet string that contains that info. Could someone remind me what that is and how you use it? Thank you! Ashley Troutman BS, HT(ASCP)QIHC Histopathology Laboratory Department of Pathology Vanderbilt University Medical Center Nashville, TN From gayle.callis <@t> bresnan.net Tue Jul 15 18:35:13 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Tue Jul 15 18:35:21 2008 Subject: [Histonet] Autofluorescence blocking ---- LONG REPLY References: <37DEF9AF72994947AF693956A59B9B660127FF22@mailbe03.mc.vanderbilt.edu> Message-ID: <001901c8e6d3$6def5af0$6401a8c0@DHXTS541> One of the best reviews of autofluorescence and how to get rid of it in found on www.IHCworld website. There is a link to a wonderful discussion on this from a group in Toronto if you go to to the fluorescence topic in IHCworld. I also have a review of autofluorescence pertaining to GFP but it applies to immunofluorescent staining too. If you want that, I will forward privately. The Toronto group discusses the different sources of autofluorescence from naturally occuring to what is induced by aldehydes, plus suggestions for eliminating each type. If you want, I can also forward this pdf to you. I have put together a document from all Histonet responses pertaining to this problem, so forgive the long cut and pasted, sometimes repeated information below. We use the 100 mM glycine method, on rehydrated sections for 20 minutes (flood section as you would with an antibody). I have seen 300 mM glycine used also, at same pH and buffer. 100 mM has worked well for us. Good luck and hope you have the proper glowing results. I have not given recognition to the gentleman who initially put this together, but I am grateful he did it. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 1. After rehydrating your (formalin fixed or paraformaldehyde fixed paraffin embedded sections, OR NBF or PFA fixed frozen sections) and before blocking for protein incubate them in 100 mM glycine for 20 minutes. This will quench autofluorescence caused by free aldehydes. Works like a charm, I use it . 2. I've pretty much moved away from doing IF on paraffin embedded stuff (ICC yes) but I have had some experience trying to knock back autofluorescence (endogenous and fix related) in tissue sections.I've had some luck pre-treating fixed sections w/ one of the following:1. 50mM Ammonium chloride in PBS for 10 min.2. 0.1M Glycine in PBS, pH 7.4 for 5-10 min.3. 1% Sodium borohydride in PBS for 10-20 min. It varies from sample to sample which method works the best but I've had the most success w/ the borohydride and NH4Cl methods.Autofluorescence can be brought on by certain endogenous tissue constituents, ie. fibronectin, lipofuscin and elastin, as well as by fixation in aldehydes. You don't say if your sections are fixed or not. If so, you should look at using sodium borohydride (0.5mg/ml in PBS) for 5 minutes (glutaraldehyde) or PBS plus a few drops of 1M glycine(formaldehyde) to block any reactive groups. ***Sodium borohydride is flammable on contact with water, and harmful by ingestion, inhalation etc. Take adequate precautions*** Another thing to consider is reducing the section thickness, if possible, as the intensity of autofluorescence is related to this. You also don't mention what fluorochromes you are using. It may be worthwhile trying a fluorochrome of a longer wavelength as there is less likelihood of any spectral overlap with the endogenous material. As I mentioned in an earlier posting today. we have had good results switching to the Alexa dyes (Molecular Probes). There are a couple of simple things you can do to help reduce autofluorescence.Some of the chemical reactions causing autofluorescence occur most rapidly with higher temperatures and on exposure to light. Therefore, performing the labelingat 4 C in the dark can help reduce this problem.Autofluorescence intensity is related to section thickness. You may want to try thinner sections if at all possible. Sometimes using fluorophores excited at longer wavelengths can help diminish autofluorescence.If autofluorescence is still an issue, there are a few preincubation steps you could try. A Tris-glycine mixture (adjust 0.1M glycine to pH 7.2-7.4 with 1M Tris base) will saturate free aldehyde groups. (15-30 minutes at room temp in Tris-glycine.Wash well in PBS. The use of 1% sodium borohydride in PBS helps reduce any free aldehyde groups in the tissue, making them non-reactive. Incubate sections for 30 minutes inborohydride and then wash well(minimum 15 minutes) in several changes of PBS.Proceed with labeling. These techniques can be used alone or sequentially. If the tissue is fragile though, only use the Tris-glycine method. Please note that sodium borohydride is very reactive and is flammable on contact with water. Another technique to block unreacted groups is to incubate sections for 5 minutes in 50mM NH4Cl, and rinse in PBS before labeling. ----- Original Message ----- From: "Troutman, Kenneth A" To: "Histonet" Sent: Tuesday, July 15, 2008 4:12 PM Subject: [Histonet] Autofluorescence blocking Dear Histonetters, I read awhile back about a reagent that you can use to block autofluorescence in tissue, but I cannot find the histonet string that contains that info. Could someone remind me what that is and how you use it? Thank you! Ashley Troutman BS, HT(ASCP)QIHC Histopathology Laboratory Department of Pathology Vanderbilt University Medical Center Nashville, TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lalit <@t> wii.gov.in Tue Jul 15 11:08:20 2008 From: lalit <@t> wii.gov.in (lalit@wii.gov.in) Date: Wed Jul 16 01:23:53 2008 Subject: [Histonet] (no subject) Message-ID: <200807151608.m6FG8Kfb002855@s1.wii.gov.in> Please un suscribe me grom this mailing group thanks -- Lalit Kumar Sharma Junior Research Fellow (JRF), Deptt-Endangered Species Management, Wildlife Institute of India, Dehradun Mob# +91-9419020101 From CBraaten <@t> Cheshire-Med.COM Wed Jul 16 08:11:48 2008 From: CBraaten <@t> Cheshire-Med.COM (Christine I. Braaten) Date: Wed Jul 16 08:11:53 2008 Subject: [Histonet] Restaining IHC slides Message-ID: Good morning histonet subscribers! I have a question about restaining previously stained slides for IHC. I know I have seen this before but cannot access the archives. If I have already stained a slide for NSE can I destain it and stain it with a different antibody? They have different retrievals so should I retrieve again? Thanks in advance for your replies. Christine CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. From rjbuesa <@t> yahoo.com Wed Jul 16 08:18:36 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jul 16 08:18:42 2008 Subject: [Histonet] Restaining IHC slides In-Reply-To: Message-ID: <465665.4366.qm@web65701.mail.ac4.yahoo.com> If the new antibody you are going to use reacts in a different place as NSE does, it would be better to just leave the NSE as is, do the new retrieval and new antibody. If the new Ab is going to react?in the same place as NSE did, you are in trouble because any "destainig" always is going to be, in the best case, just partial (incomplete). Ren? J. --- On Wed, 7/16/08, Christine I. Braaten wrote: From: Christine I. Braaten Subject: [Histonet] Restaining IHC slides To: histonet@lists.utsouthwestern.edu Date: Wednesday, July 16, 2008, 9:11 AM Good morning histonet subscribers! I have a question about restaining previously stained slides for IHC. I know I have seen this before but cannot access the archives. If I have already stained a slide for NSE can I destain it and stain it with a different antibody? They have different retrievals so should I retrieve again? Thanks in advance for your replies. Christine CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Saro.Bascaramurty <@t> nrc-cnrc.gc.ca Wed Jul 16 08:57:02 2008 From: Saro.Bascaramurty <@t> nrc-cnrc.gc.ca (Bascaramurty, Saro) Date: Wed Jul 16 08:57:07 2008 Subject: [Histonet] Help Message-ID: <00E9EC516E0CEB4A9ED53E7FAD19DE090235474E@nrccenexb1.nrc.ca> Hi histonet users, I am looking for an ideal protocol to fix and process tissue blocks of blood vessels (coronary and aorta) from rabbit (aged 22 months and 6 months) for paraffin embedding. I would greatly appreciate your help on this. Thanks, Saro Bascaramurty email:saro.bascaramurty@nrc-cnrc.gc.ca From Rcartun <@t> harthosp.org Wed Jul 16 09:01:07 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Jul 16 09:01:19 2008 Subject: [Histonet] Restaining IHC slides In-Reply-To: References: Message-ID: <487DC6E40200007700004149@gwmail6.harthosp.org> Yes, we do this often. You need to make sure that you can differentiate between the two reactivities (e.g., cytoplasmic vs. nuclear) if you have any permanent reactivity (DAB) from the first antibody. Alternatively, you could use a different chromogen for the second antibody. Yes, you will need to retrieve again. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Christine I. Braaten" 07/16/08 9:11 AM >>> Good morning histonet subscribers! I have a question about restaining previously stained slides for IHC. I know I have seen this before but cannot access the archives. If I have already stained a slide for NSE can I destain it and stain it with a different antibody? They have different retrievals so should I retrieve again? Thanks in advance for your replies. Christine CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tifei <@t> foxmail.com Wed Jul 16 09:35:32 2008 From: tifei <@t> foxmail.com (tf) Date: Wed Jul 16 09:35:47 2008 Subject: [Histonet] Rat-source primary antibody on Rat tissue References: <00E9EC516E0CEB4A9ED53E7FAD19DE090235474E@nrccenexb1.nrc.ca> Message-ID: <200807162235273849534@foxmail.com> Dear all: I am writing to discuss the use of rat-sourced primary antibody on rat tissue. One concern (from Andrea Hooper's last email to me) is that the 10mg/ml IgG in normal blood will result in great background, or false-positive staining. How do you think? My personal opinions are: (1) perfuse the rat well. (2) use a control group without 1st antibody, just add goat-anti-Rat IgG, for example (care about the cross reactivity) (3) use DAPI staining to see if the staings are located in cell, rather by blood contamination. 2008-07-16 tf From anh2006 <@t> med.cornell.edu Wed Jul 16 09:50:40 2008 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Wed Jul 16 09:53:31 2008 Subject: [Histonet] Rat-source primary antibody on Rat tissue Message-ID: <1083151490-1216219997-cardhu_decombobulator_blackberry.rim.net-1980208217-@bxe157.bisx.prod.on.blackberry> UGVyZnVzaW9uIHdpbGwgZGVmaW5pdGVseSBoZWxwLg0KDQpVc2luZyBjb250cm9scyBpcyBhYnNv bHV0ZWx5IGVzc2VudGlhbCBpbiBhbnkgZXhwZXJpbWVudCBhbmQgd2lsbCBoZWxwIGRldGVybWlu ZSB3aGF0IGlzIGZhbHNlIHBvc2l0aXZlIGJ1dCBpdCB3aWxsIG5vdCBzaG93IGZhbHNlIKHobmVn YXRpdmVzoeguIElmIHlvdSBhcmUgc3RhaW5pbmcgYSBibG9vZHkgdGlzc3VlIGxpa2UgdW5wZXJm dXNlZCBoZWFydCBldGMgdGhlIGJsb29kIElnRyB3aWxsIGJpbmQgdG8geW91IHNlY29uZGFyeSBh YnMgYW5kIGl0IGlzIHBvc3NpYmxlIHRoYXQgeW91IHdpbGwgbm90IGJlIGFibGUgdG8gZGV0ZWN0 IHlvdSBhbnRpZ2VuIG9yIGl0IHdpbGwgYmUgd2Vha2x5IHN0YWluZWQuIEdldCByaWQgb2YgdGhh dCBiYWNrZ3JvdW5kIGFuZCB0aGUgcG9zaXRpdmUgc3RhaW4gd291bGQgJ3BvcCcuDQoNClVzaW5n IGRhcGkgc3RhaW5pbmcgaXMgZmluZSwgeW91IHNob3VsZCBwcm9iYWJseSBiZSBkb2luZyB0aGlz IGFueXdheSwgYnV0IHRoZSBwcm9ibGVtIG9mIHBvb3Igc3RhaW5pbmcgcXVhbGl0eSBzdGlsbCBy ZW1haW5zICh1bmxlc3MgeW91ciB0aXNzdWVzIGFyZSB3ZWxsLXBlcmZ1c2VkKS4NCg0KSSB3b3Vs ZCBjb25zaWRlciB0byBkbyB0aGlzIG9ubHkgaW4gdGhlIG1vc3QgZGVzcGVyYXRlIG9mIGNhc2Vz Lg0KDQotLS0tLS1PcmlnaW5hbCBNZXNzYWdlLS0tLS0tDQpGcm9tOiB0Zg0KU2VuZGVyOiBoaXN0 b25ldC1ib3VuY2VzQGxpc3RzLnV0c291dGh3ZXN0ZXJuLmVkdQ0KVG86IGhpc3RvbmV0QGxpc3Rz LnV0c291dGh3ZXN0ZXJuLmVkDQpSZXBseVRvOiB0aWZlaUBmb3htYWlsLmNvbQ0KU2VudDogSnVs IDE2LCAyMDA4IDEwOjM1IEFNDQpTdWJqZWN0OiBbSGlzdG9uZXRdIFJhdC1zb3VyY2UgcHJpbWFy eSBhbnRpYm9keSBvbiBSYXQgdGlzc3VlDQoNCkRlYXIgYWxsOg0KDQpJIGFtIHdyaXRpbmcgdG8g ZGlzY3VzcyB0aGUgdXNlIG9mIHJhdC1zb3VyY2VkIHByaW1hcnkgYW50aWJvZHkgb24gcmF0IHRp c3N1ZS4NCk9uZSBjb25jZXJuIChmcm9tIEFuZHJlYSBIb29wZXIncyBsYXN0IGVtYWlsIHRvIG1l KSBpcyB0aGF0IHRoZSAxMG1nL21sIElnRyBpbiBub3JtYWwgYmxvb2Qgd2lsbCByZXN1bHQgaW4g Z3JlYXQgYmFja2dyb3VuZCwgb3IgZmFsc2UtcG9zaXRpdmUgc3RhaW5pbmcuDQpIb3cgZG8geW91 IHRoaW5rPw0KDQpNeSBwZXJzb25hbCBvcGluaW9ucyBhcmU6DQoNCigxKSBwZXJmdXNlIHRoZSBy YXQgd2VsbC4NCigyKSB1c2UgYSBjb250cm9sIGdyb3VwIHdpdGhvdXQgMXN0IGFudGlib2R5LCBq dXN0IGFkZCBnb2F0LWFudGktUmF0IElnRywgZm9yIGV4YW1wbGUgKGNhcmUgYWJvdXQgdGhlIGNy b3NzIHJlYWN0aXZpdHkpDQooMykgdXNlIERBUEkgc3RhaW5pbmcgdG8gc2VlIGlmIHRoZSBzdGFp bmdzIGFyZSBsb2NhdGVkIGluIGNlbGwsIHJhdGhlciBieSBibG9vZCBjb250YW1pbmF0aW9uLg0K DQoNCjIwMDgtMDctMTYgDQoNCg0KDQp0ZiANCl9fX19fX19fX19fX19fX19fX19fX19fX19fX19f X19fX19fX19fX19fX19fX19fDQpIaXN0b25ldCBtYWlsaW5nIGxpc3QNCkhpc3RvbmV0QGxpc3Rz LnV0c291dGh3ZXN0ZXJuLmVkdQ0KaHR0cDovL2xpc3RzLnV0c291dGh3ZXN0ZXJuLmVkdS9tYWls bWFuL2xpc3RpbmZvL2hpc3RvbmV0DQoNCg== From NMargaryan <@t> childrensmemorial.org Wed Jul 16 11:54:24 2008 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Wed Jul 16 11:55:21 2008 Subject: [Histonet] IgG goat Message-ID: Hi Dears, I have to order IgG goat for my negative controls (IHC, paraffin sections). Please let me know what company is the best and don't show any background staining. Thanks in advance, Naira Naira V. Margaryan, D.V.M., Ph.D. Research Scientist Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3363 Tel: 773-755-6340 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 From Beatrice.Debrosse-Serra <@t> pfizer.com Wed Jul 16 12:38:16 2008 From: Beatrice.Debrosse-Serra <@t> pfizer.com (Debrosse-Serra, Beatrice) Date: Wed Jul 16 12:38:38 2008 Subject: [Histonet] IgG goat In-Reply-To: Message-ID: <7B41B921086ADE4186377B8C33F702DE0668DA25@lajamrexm01.amer.pfizer.com> I would be interested in this as well. Beatrice DeBrosse-Serra Pathology Scientist Pfizer Global Research & Development CB4, 2150 10646 Science Center Drive San Diego, CA 92121 Phone# 858-622-5986 Fax# 858-678-8290 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Wednesday, July 16, 2008 9:54 AM To: histonet@lists.utsouthwestern.edu Cc: lgruman@mcw.edu Subject: [Histonet] IgG goat Hi Dears, I have to order IgG goat for my negative controls (IHC, paraffin sections). Please let me know what company is the best and don't show any background staining. Thanks in advance, Naira Naira V. Margaryan, D.V.M., Ph.D. Research Scientist Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3363 Tel: 773-755-6340 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sheila_adey <@t> hotmail.com Wed Jul 16 12:52:22 2008 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Wed Jul 16 12:52:27 2008 Subject: [Histonet] Biogenex i6000 users Message-ID: Hello netters, Could any Biogenex i6000 users please contact me regarding protocols for the IHC portion of the instrument. We have inconsistency in staining and unhappy pathologists. The issues mostly seem to be with the CK AE1 and S100. Thanks in advance _________________________________________________________________ From anh2006 <@t> med.cornell.edu Wed Jul 16 13:06:24 2008 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Wed Jul 16 13:06:51 2008 Subject: [Histonet] IgG goat In-Reply-To: References: Message-ID: Jackson ImmunoResearch. >Hi Dears, > > > >I have to order IgG goat for my negative controls (IHC, paraffin >sections). Please let me know what company is the best and don't show >any background staining. > > -- From jmjohnson34 <@t> hotmail.com Wed Jul 16 13:29:44 2008 From: jmjohnson34 <@t> hotmail.com (Jennifer Johnson) Date: Wed Jul 16 13:29:48 2008 Subject: [Histonet] SelecTech Stains Message-ID: I have three bottles of SelecTech stains; the Alcoholic Eosin Y, the blue buffer, and the Define. I will send them to you if you give me your FedEx number. Is it alright to send 500 ml of Alcoholic Eosin Y by fedex, it has a Flammability rating of a 2? If not, send me an alternative and I will accomodate. I hate to waste! Thanks, Jennifer _________________________________________________________________ With Windows Live for mobile, your contacts travel with you. http://www.windowslive.com/mobile/overview.html?ocid=TXT_TAGLM_WL_mobile_072008 From akemiat3377 <@t> yahoo.com Wed Jul 16 15:14:24 2008 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Wed Jul 16 15:14:28 2008 Subject: [Histonet] Methodist hospital Houston, TX Message-ID: <788444.62349.qm@web31307.mail.mud.yahoo.com> Hi All, I need to get a hold of the IHC supervisor at Methodist Hospital, Houston, TX. It's been several years since I was there and don't know who it currently the IHC supervisor. Dr. Allen Gown requested I get some information. Can anyone give his/her name and contact telephone number. Thanks, Akemi Allison-Tacha, BS, HT(ASCP)HTL Client Services Manager PhenoPath laboratories 551 North 34th Street, Suite 100 Seattle, WA 98103-8675 Work: (206) 374-9000 ext 1053 E-Mail: akemiat3377@yahoo.com From PMonfils <@t> Lifespan.org Wed Jul 16 17:23:37 2008 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Jul 16 17:23:41 2008 Subject: [Histonet] Tissue-Tek cold plates? Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835C2E@LSRIEXCH1.lsmaster.lifespan.org> Are Tissue-Tek cold plates still available anywhere? - plastic, white on top, black on the bottom, filled with some kind of gel. You put them in the freezer, then take them out to keep your paraffin blocks cold. From jstaruk <@t> masshistology.com Wed Jul 16 18:01:17 2008 From: jstaruk <@t> masshistology.com (jstaruk) Date: Wed Jul 16 18:01:40 2008 Subject: [Histonet] Tissue-Tek cold plates? In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E03835C2E@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <436E72624F5D4B3AB4808C7E949D62D5@JimPC> I have some extras, how many are you looking for? Jim _____________________ Jim Staruk Mass Histology Service www.masshistology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Wednesday, July 16, 2008 6:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue-Tek cold plates? Are Tissue-Tek cold plates still available anywhere? - plastic, white on top, black on the bottom, filled with some kind of gel. You put them in the freezer, then take them out to keep your paraffin blocks cold. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terri.Brown <@t> Northside.com Thu Jul 17 07:33:15 2008 From: Terri.Brown <@t> Northside.com (Terri Brown) Date: Thu Jul 17 07:33:10 2008 Subject: [Histonet] Tissue-Tek cold plates? In-Reply-To: <436E72624F5D4B3AB4808C7E949D62D5@JimPC> References: <4EBFF65383B74D49995298C4976D1D5E03835C2E@LSRIEXCH1.lsmaster.lifespan.org> <436E72624F5D4B3AB4808C7E949D62D5@JimPC> Message-ID: <8CEB6DA1A3F35743800669D4CFE21F7D03F901BD@NSMXMS04.northside.local> You can order them from Cardinal. They come 6 per box and the catalog number is M7410-10. Cardinal's number is 1-800-964-5227. Terri H. Brown, HT (ASCP) Pathology Lab Manager Northside Hospital Atlanta, Georgia 30342 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jstaruk Sent: Wednesday, July 16, 2008 7:01 PM To: 'Monfils, Paul'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Tissue-Tek cold plates? I have some extras, how many are you looking for? Jim _____________________ Jim Staruk Mass Histology Service www.masshistology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Wednesday, July 16, 2008 6:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue-Tek cold plates? Are Tissue-Tek cold plates still available anywhere? - plastic, white on top, black on the bottom, filled with some kind of gel. You put them in the freezer, then take them out to keep your paraffin blocks cold. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. From jqb7 <@t> cdc.gov Thu Jul 17 08:28:40 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu Jul 17 08:29:47 2008 Subject: [Histonet] Tissue-Tek cold plates? In-Reply-To: <8CEB6DA1A3F35743800669D4CFE21F7D03F901BD@NSMXMS04.northside.local> References: <4EBFF65383B74D49995298C4976D1D5E03835C2E@LSRIEXCH1.lsmaster.lifespan.org> <436E72624F5D4B3AB4808C7E949D62D5@JimPC> <8CEB6DA1A3F35743800669D4CFE21F7D03F901BD@NSMXMS04.northside.local> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A7F23D10@LTA3VS011.ees.hhs.gov> Sakura sells them. http://sakura.ifuture.com/showitem.asp?ifut=1000183614529078360%2CTKWK%3 E6%3D%3A5ST0&id=44332344650354.00 Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Brown Sent: Thursday, July 17, 2008 8:33 AM To: jstaruk; Monfils, Paul; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Tissue-Tek cold plates? You can order them from Cardinal. They come 6 per box and the catalog number is M7410-10. Cardinal's number is 1-800-964-5227. Terri H. Brown, HT (ASCP) Pathology Lab Manager Northside Hospital Atlanta, Georgia 30342 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jstaruk Sent: Wednesday, July 16, 2008 7:01 PM To: 'Monfils, Paul'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Tissue-Tek cold plates? I have some extras, how many are you looking for? Jim _____________________ Jim Staruk Mass Histology Service www.masshistology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Wednesday, July 16, 2008 6:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue-Tek cold plates? Are Tissue-Tek cold plates still available anywhere? - plastic, white on top, black on the bottom, filled with some kind of gel. You put them in the freezer, then take them out to keep your paraffin blocks cold. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Thu Jul 17 08:59:26 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Thu Jul 17 08:59:32 2008 Subject: [Histonet] Tissue-Tek cold plates? Message-ID: <57BE698966D5C54EAE8612E8941D76830360233B@EXCHANGE3.huntingtonhospital.com> Yes, they're still available. I just ordered some through Cardinal. There item # is M7410-10 and there are 6 trays per pack. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Wednesday, July 16, 2008 3:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue-Tek cold plates? Are Tissue-Tek cold plates still available anywhere? - plastic, white on top, black on the bottom, filled with some kind of gel. You put them in the freezer, then take them out to keep your paraffin blocks cold. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Heather.D.Renko <@t> osfhealthcare.org Thu Jul 17 09:52:26 2008 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Thu Jul 17 09:52:44 2008 Subject: [Histonet] re: Old processor Message-ID: <40026EDDE64CDA47AB382C52619ACD3C0C44A029@pmc-rfd-mx01.intranet.osfnet.org> I have a new processor and now an old, old one to get out of storage: VIP 2000 Tissue-Tek Model #4618 SN: 8922617 This instrument is about twenty-one years old and has been maintained but, not used regularly for about the last ten years. Email me direct if your want to inquire further on it. Thank you, Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From denise.brinson <@t> yahoo.com Thu Jul 17 09:56:55 2008 From: denise.brinson <@t> yahoo.com (Denise Brinson) Date: Thu Jul 17 09:57:02 2008 Subject: [Histonet] Histology postion Message-ID: <468416.37735.qm@web46110.mail.sp1.yahoo.com> Hello! ? Is anyone aware of any Histology position in the Eastern Tennessee area? ? Best regards, ? C.D. Brinson denise.brinson@yahoo.com 865-292-3951 From jstaruk <@t> masshistology.com Thu Jul 17 10:18:56 2008 From: jstaruk <@t> masshistology.com (jstaruk) Date: Thu Jul 17 10:19:16 2008 Subject: [Histonet] Histology opening in central Massachusetts In-Reply-To: <468416.37735.qm@web46110.mail.sp1.yahoo.com> Message-ID: I have at least one, perhaps two openings in this ever-growing private histolab. One position is for routine histotechniques and the other is for grossing veterinary surgicals and biotech specimens. Hours are flexible, pay and benefits are excellent and we're pretty laid back here (most of the time). Anyone out there interested? Please email me. Jim _____________________ Jim Staruk Mass Histology Service www.masshistology.com From Loralee_Mcmahon <@t> URMC.Rochester.edu Thu Jul 17 10:38:02 2008 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Thu Jul 17 10:40:49 2008 Subject: [Histonet] CD4 Fluorescent Staining Message-ID: <2CF6F6B05263EA4EBAB07781B51E5DB0025A280A@e2k3ms1.urmc-sh.rochester.edu> I am trying an antibody called Alexa Fluor 488 anti-mouse CD4 on fresh frozen mouse tissue. Having only worked with AED/DAB staining, not very familiar with this. Does anyone have any experience with this and if you could send me some tips/protocols. Thanks ahead of time Loralee McMahon, HTL (ASCP) ICC Supervisor University of Rochester Department of Pathology (585) 275-7210 From rmweber113 <@t> comcast.net Thu Jul 17 11:07:13 2008 From: rmweber113 <@t> comcast.net (rmweber113@comcast.net) Date: Thu Jul 17 11:07:19 2008 Subject: [Histonet] JOB IN NJ Message-ID: <071720081607.11883.487F6E31000C6DD900002E6B2216527966CCCECE9D0A0D0A99039D@comcast.net> I have a job available for a experienced certified histologist in Voorhees NJ. Candidate must be detailed oriented, well organized, have good communication skills and can work independently. Pease email me your resume or call me at 732 814-1170 Thank you, Marilynn Weber From akbitting <@t> geisinger.edu Thu Jul 17 11:13:08 2008 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Thu Jul 17 11:13:34 2008 Subject: [Histonet] Xpress Message-ID: <487F3754.2B7F.00C9.0@geisinger.edu> Would someone be willing to share their experience with processing breast needle bxs on the Sakura Xpress processor? Call me or email me. Either is fine. Thanks, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From juditw <@t> u.washington.edu Thu Jul 17 11:26:27 2008 From: juditw <@t> u.washington.edu (Judith L. Williams) Date: Thu Jul 17 11:26:34 2008 Subject: [Histonet] Saturated Fat Staining In-Reply-To: Message-ID: Hi- Check in to Nile Red or Nile blue if you do not mind doing fluorescent microscopy. It works well on saturated fats and is quite specific. good luck. Judy Williams Comparative Medicine U of Washington On Fri, 11 Jul 2008, Griffin-Reyes, Michelle A wrote: > I am having some troubles finding a way to be able to stain saturated > lipids in FFPE mouse tissues. Osmium tetroxide and oil red O stain well > but they are only showing unsaturated lipids. My lab is looking to see > if there is a difference in lipid content of treated mice vs. control > mice. So far, we have seen very little variability in the amount of > unsaturated lipids during quantification which leads us to believe there > may be a difference in saturated fats (based off of human results that > we have our mouse model based off of). Does anyone have any suggestions? > Thanks so much for the help! > > Michelle A. Griffin-Reyes > Comparative Pathology Laboratory > University of Iowa: College of Medicine > > > > Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Judith Williams, PhD, HT(ASCP) Research Scientist Department of Comparative Medicine University of Washington Seattle, WA 98195 From gu.lang <@t> gmx.at Thu Jul 17 11:30:44 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Jul 17 11:31:02 2008 Subject: [Histonet] hcv and mastcells Message-ID: <5EB233BCA05B4CE0A1336756E4C4B44D@dielangs.at> Hi all! I did an immunohistochemistry for heptatitis C virus on skin of a man, who formerly had an hcv infection. The mastcells showed a granular staining in the cytoplasm. Has anyone experience or explanations with this phenomen? Could it be due to a cross-reaction or are there really viruses (at least particel) in the mastcell-granula? Thanks in advance Gudrun Lang From gu.lang <@t> gmx.at Thu Jul 17 11:33:32 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Jul 17 11:33:52 2008 Subject: [Histonet] borrelia burgdorferi protocol Message-ID: Hi all! Can anyone provide me a borrelia burgdorferi protocol for ihc, preferable on the Benchmark XT? We will purchase a polyclonal antibody from Acris. In the datasheet is no hint about pretreatment or titer. So some information would be helpful to start with. Thanks in advance Gudrun Lang From JEllin <@t> yumaregional.org Thu Jul 17 11:39:44 2008 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Thu Jul 17 11:39:49 2008 Subject: [Histonet] ER,PR, HER2/Neu In-Reply-To: <5EB233BCA05B4CE0A1336756E4C4B44D@dielangs.at> Message-ID: <29BE166A2CF48D459853F8EC57CD37E8011D8956@EXCHANGECLUSTER.yumaregional.local> We are in the early stages of getting into ER/PR/HER2Neu scanning technology using the Ventana Image Analysis System (VIAS). Wanting to know the Pit falls and also what else to expect when going into this realm,, Any information would greatly be appreciated. Jesus Ellin HT/PA ASCP Yuma Regional Medical Center 928-336-1743 This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. From tifei <@t> foxmail.com Thu Jul 17 11:50:42 2008 From: tifei <@t> foxmail.com (tf) Date: Thu Jul 17 11:51:09 2008 Subject: [Histonet] Saturated Fat Staining References: Message-ID: <200807180050371563194@foxmail.com> QW55IHByZWNpc2UgcHJvdG9jb2wgZm9yIEZhdCB0aXNzdWUgc3RhaW5pbmc/DQoNClRoZSBwcm9j ZXNzaW5nIHByb2NlZHVyZT8NCnRvIHJlbW92ZSBmYXQsIGJlZm9yZSBpbW11bm9zdGFpbmluZz8N Cg0KDQoyMDA4LTA3LTE4IA0KDQoNCg0KdGYgDQoNCg0KDQq3orz+yMujuiBKdWRpdGggTC4gV2ls bGlhbXMgDQq3osvNyrG85KO6IDIwMDgtMDctMTggIDAwOjMxOjUwIA0KytW8/sjLo7ogR3JpZmZp bi1SZXllcywgTWljaGVsbGUgQSANCrOty82juiBoaXN0b25ldEBsaXN0cy51dHNvdXRod2VzdGVy bi5lZHUgDQrW98zio7ogUmU6IFtIaXN0b25ldF0gU2F0dXJhdGVkIEZhdCBTdGFpbmluZyANCiAN CkhpLSBDaGVjayBpbiB0byBOaWxlIFJlZCBvciBOaWxlIGJsdWUgaWYgeW91IGRvIG5vdCBtaW5k IGRvaW5nIGZsdW9yZXNjZW50IG1pY3Jvc2NvcHkuIEl0IHdvcmtzIHdlbGwgb24gc2F0dXJhdGVk IGZhdHMgYW5kIGlzIHF1aXRlIHNwZWNpZmljLiBnb29kIGx1Y2suDQpKdWR5IFdpbGxpYW1zDQpD b21wYXJhdGl2ZSBNZWRpY2luZQ0KVSBvZiBXYXNoaW5ndG9uDQpPbiBGcmksIDExIEp1bCAyMDA4 LCBHcmlmZmluLVJleWVzLCBNaWNoZWxsZSBBIHdyb3RlOg0KPiBJIGFtIGhhdmluZyBzb21lIHRy b3VibGVzIGZpbmRpbmcgYSB3YXkgdG8gYmUgYWJsZSB0byBzdGFpbiBzYXR1cmF0ZWQNCj4gbGlw aWRzIGluIEZGUEUgbW91c2UgdGlzc3Vlcy4gT3NtaXVtIHRldHJveGlkZSBhbmQgb2lsIHJlZCBP IHN0YWluIHdlbGwNCj4gYnV0IHRoZXkgYXJlIG9ubHkgc2hvd2luZyB1bnNhdHVyYXRlZCBsaXBp ZHMuIE15IGxhYiBpcyBsb29raW5nIHRvIHNlZQ0KPiBpZiB0aGVyZSBpcyBhIGRpZmZlcmVuY2Ug aW4gbGlwaWQgY29udGVudCBvZiB0cmVhdGVkIG1pY2UgdnMuIGNvbnRyb2wNCj4gbWljZS4gU28g ZmFyLCB3ZSBoYXZlIHNlZW4gdmVyeSBsaXR0bGUgdmFyaWFiaWxpdHkgaW4gdGhlIGFtb3VudCBv Zg0KPiB1bnNhdHVyYXRlZCBsaXBpZHMgZHVyaW5nIHF1YW50aWZpY2F0aW9uIHdoaWNoIGxlYWRz IHVzIHRvIGJlbGlldmUgdGhlcmUNCj4gbWF5IGJlIGEgZGlmZmVyZW5jZSBpbiBzYXR1cmF0ZWQg ZmF0cyAoYmFzZWQgb2ZmIG9mIGh1bWFuIHJlc3VsdHMgdGhhdA0KPiB3ZSBoYXZlIG91ciBtb3Vz ZSBtb2RlbCBiYXNlZCBvZmYgb2YpLiBEb2VzIGFueW9uZSBoYXZlIGFueSBzdWdnZXN0aW9ucz8N Cj4gVGhhbmtzIHNvIG11Y2ggZm9yIHRoZSBoZWxwIQ0KPg0KPiBNaWNoZWxsZSBBLiBHcmlmZmlu LVJleWVzDQo+IENvbXBhcmF0aXZlIFBhdGhvbG9neSBMYWJvcmF0b3J5DQo+IFVuaXZlcnNpdHkg b2YgSW93YTogQ29sbGVnZSBvZiBNZWRpY2luZQ0KPg0KPg0KPg0KPiBOb3RpY2U6IFRoaXMgVUkg SGVhbHRoIENhcmUgZS1tYWlsIChpbmNsdWRpbmcgYXR0YWNobWVudHMpIGlzIGNvdmVyZWQgYnkg dGhlIEVsZWN0cm9uaWMgQ29tbXVuaWNhdGlvbnMgUHJpdmFjeSBBY3QsIDE4IFUuUy5DLiAyNTEw LTI1MjEsIGlzIGNvbmZpZGVudGlhbCBhbmQgbWF5IGJlIGxlZ2FsbHkgcHJpdmlsZWdlZC4gIElm IHlvdSBhcmUgbm90IHRoZSBpbnRlbmRlZCByZWNpcGllbnQsIHlvdSBhcmUgaGVyZWJ5IG5vdGlm aWVkIHRoYXQgYW55IHJldGVudGlvbiwgZGlzc2VtaW5hdGlvbiwgZGlzdHJpYnV0aW9uLCBvciBj b3B5aW5nIG9mIHRoaXMgY29tbXVuaWNhdGlvbiBpcyBzdHJpY3RseSBwcm9oaWJpdGVkLiAgUGxl YXNlIHJlcGx5IHRvIHRoZSBzZW5kZXIgdGhhdCB5b3UgaGF2ZSByZWNlaXZlZCB0aGUgbWVzc2Fn ZSBpbiBlcnJvciwgdGhlbiBkZWxldGUgaXQuICBUaGFuayB5b3UuDQo+DQo+IF9fX19fX19fX19f X19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fDQo+IEhpc3RvbmV0IG1haWxpbmcg bGlzdA0KPiBIaXN0b25ldEBsaXN0cy51dHNvdXRod2VzdGVybi5lZHUNCj4gaHR0cDovL2xpc3Rz LnV0c291dGh3ZXN0ZXJuLmVkdS9tYWlsbWFuL2xpc3RpbmZvL2hpc3RvbmV0DQo+DQpKdWRpdGgg V2lsbGlhbXMsIFBoRCwgSFQoQVNDUCkNClJlc2VhcmNoIFNjaWVudGlzdA0KRGVwYXJ0bWVudCBv ZiBDb21wYXJhdGl2ZSBNZWRpY2luZQ0KVW5pdmVyc2l0eSBvZiBXYXNoaW5ndG9uDQpTZWF0dGxl LCBXQSA5ODE5NQ0KX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19f X18NCkhpc3RvbmV0IG1haWxpbmcgbGlzdA0KSGlzdG9uZXRAbGlzdHMudXRzb3V0aHdlc3Rlcm4u ZWR1DQo= From elizabeth.heimrich <@t> bms.com Thu Jul 17 13:01:16 2008 From: elizabeth.heimrich <@t> bms.com (Elizabeth M Heimrich) Date: Thu Jul 17 13:01:44 2008 Subject: [Histonet] LCM on decaled bone sections Message-ID: <487F88EC.8010505@bms.com> Hello everyone, I have a question regarding the doability of LCM on decaled rat joint specimens and the subsequent RNA extraction and amplification. The rat joints have not been harvested yet, so there is no fixative decided upon. Is this a feasible procedure? Does the decalcification of the bone destroy the RNA, or is the main issue the formaldehyde cross-linking the proteins if using FFPE tissue. I have queried a few scientists around here and have received conflicting answers, so I come to the net....hoping to resolve this issue. Some say to dissect out the synovium and take cryo sections because the Formaldehyde will interfere with the RNA signal. Others say that the problem resides with the decal procedure itself. Any and all thoughts welcome. Thank you, Beth From Rcartun <@t> harthosp.org Thu Jul 17 14:00:19 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Jul 17 14:00:31 2008 Subject: [Histonet] borrelia burgdorferi protocol In-Reply-To: References: Message-ID: <487F5E8302000077000042AD@gwmail6.harthosp.org> I have been doing IHC for Borrelia burgdorferi for years and have never seen a positive human case; only in animal tissue. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Gudrun Lang" 07/17/08 12:33 PM >>> Hi all! Can anyone provide me a borrelia burgdorferi protocol for ihc, preferable on the Benchmark XT? We will purchase a polyclonal antibody from Acris. In the datasheet is no hint about pretreatment or titer. So some information would be helpful to start with. Thanks in advance Gudrun Lang From gu.lang <@t> gmx.at Thu Jul 17 14:09:26 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Jul 17 14:09:46 2008 Subject: AW: [Histonet] borrelia burgdorferi protocol In-Reply-To: <487F5E8302000077000042AD@gwmail6.harthosp.org> Message-ID: <37E7D6EF2B8D440DB16B613E1E6D7A87@dielangs.at> In spite of your "negative experiences" would you please share your protocol with me? And what do you think is the cause, why it doesn't work on human tissue? Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: Richard Cartun [mailto:Rcartun@harthosp.org] Gesendet: Donnerstag, 17. Juli 2008 21:00 An: gu.lang@gmx.at; histonet@lists.utsouthwestern.edu Betreff: Re: [Histonet] borrelia burgdorferi protocol I have been doing IHC for Borrelia burgdorferi for years and have never seen a positive human case; only in animal tissue. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Gudrun Lang" 07/17/08 12:33 PM >>> Hi all! Can anyone provide me a borrelia burgdorferi protocol for ihc, preferable on the Benchmark XT? We will purchase a polyclonal antibody from Acris. In the datasheet is no hint about pretreatment or titer. So some information would be helpful to start with. Thanks in advance Gudrun Lang From cbritos <@t> pathsrv.com Thu Jul 17 14:26:02 2008 From: cbritos <@t> pathsrv.com (Cristian Britos) Date: Thu Jul 17 14:26:08 2008 Subject: [Histonet] AP Cerner Copath vs Sunquest Copath vs others In-Reply-To: <37E7D6EF2B8D440DB16B613E1E6D7A87@dielangs.at> References: <487F5E8302000077000042AD@gwmail6.harthosp.org> <37E7D6EF2B8D440DB16B613E1E6D7A87@dielangs.at> Message-ID: Would someone be willing to share their experiences about Sunquest copath as their LIS system for pathology versus other systems? Needless to say, we are looking for options.... (currently we have cerner copath 3.0) Thanks, Cris From Herrick.James <@t> mayo.edu Thu Jul 17 15:16:09 2008 From: Herrick.James <@t> mayo.edu (Herrick, James L.) Date: Thu Jul 17 15:16:13 2008 Subject: [Histonet] Question on Aluminum Staining Message-ID: Hi everyone, We are having trouble running ASA (acid solochrome azurin) stains on human iliac crest bone biopsies for the detection of aluminum deposits. Our negatives are showing a lot of blue around the outer edges of the specimen. Although we cannot see staining around the trabecular bone, we should also not see it around the periphery of the bone specimen. The positive, on the other hand, looks very good. Has anyone had experience with this type of stain, and if so, would you happen to know what we may be doing wrong? The recipe we use for our stain is .2 gms. of Chromeazural B in 20mL of dH2O with a pH @ 5.0 (We incubate at room temp for 18hrs.), destain with 95% methanol, rinse in dH2O, dehydrate, clear and coverslip. Also, another problem we have encountered in the past, is a gold discoloring of the specimen. Have you seen this problem before, and if so, do you know how to correct it? Any help we can get with this dilemma is very much appreciated. Thanks again, Jim From gayle.callis <@t> bresnan.net Thu Jul 17 15:41:37 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Thu Jul 17 15:41:42 2008 Subject: [Histonet] CD4 Fluorescent Staining References: <2CF6F6B05263EA4EBAB07781B51E5DB0025A280A@e2k3ms1.urmc-sh.rochester.edu> Message-ID: <006801c8e84d$844c5520$6401a8c0@DHXTS541> Just to clarify exactly what you are doing - are you using a rat antiMouse CD4 conjugated to Alexa 488? After I get the answer, I will elaborate. We do successful CD4 immunofluorescent staining but never use a CD4 primary direct, with the primary conjugated to the fluorphore. We have four different ways of doing the staining. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT ----- Original Message ----- From: "McMahon, Loralee A" To: Sent: Thursday, July 17, 2008 9:38 AM Subject: [Histonet] CD4 Fluorescent Staining I am trying an antibody called Alexa Fluor 488 anti-mouse CD4 on fresh frozen mouse tissue. Having only worked with AED/DAB staining, not very familiar with this. Does anyone have any experience with this and if you could send me some tips/protocols. Thanks ahead of time Loralee McMahon, HTL (ASCP) ICC Supervisor University of Rochester Department of Pathology (585) 275-7210 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Thu Jul 17 16:39:27 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Jul 17 16:39:46 2008 Subject: [Histonet] Formlain containers Message-ID: <487F83CF02000077000042C8@gwmail6.harthosp.org> Does anyone receive tissue specimens in plastic bags containing formalin? We do and I don't like it. For some reason this client likes to use these plastic bags instead of our standard formalin bottles. The bags have to be cut open and are difficult to store. The person in the gross room ends up placing all remaining tissue in one of our regular specimen bottles after completing the gross examination. I am going to tell them that we are not going to accept the specimens unless you can convince me that it is O.K. (and I don't think that is going to happen). Thanks for your time. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From ploykasek <@t> phenopath.com Thu Jul 17 16:48:18 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Thu Jul 17 16:48:27 2008 Subject: [Histonet] Desmoglein 3 antibody Message-ID: Hi all. We have been trying to work up an antibody, desmoglein 3, in squamous cell lung carcinoma. This is based on an article in Feb. 2007 Human Pathology. We have had great difficulty in optimizing the antibody in FFPE. If anyone else has this antibody up & working well, I would appreciate any help with it. Thanks. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From gmartin <@t> marshallmedical.org Thu Jul 17 17:59:36 2008 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Thu Jul 17 17:59:49 2008 Subject: [Histonet] Formlain containers In-Reply-To: <487F83CF02000077000042C8@gwmail6.harthosp.org> References: <487F83CF02000077000042C8@gwmail6.harthosp.org> Message-ID: <6ED9D4252F278841A0593D3D788AF24C02DFF0D2@mailsvr.MARSHMED.local> We use both ... the bags have not been a problem ... as a matter for fact I prefer them. They seem to store easier. We also noticed that many times clients try to stuff a very large specimen into a small bottle, and fixation can suffer. The bags we use are designed for pathology use. They are manufactured by Bitran, and sold by Cardinal Health Gary -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, July 17, 2008 2:39 PM To: Histonet Subject: [Histonet] Formlain containers Does anyone receive tissue specimens in plastic bags containing formalin? We do and I don't like it. For some reason this client likes to use these plastic bags instead of our standard formalin bottles. The bags have to be cut open and are difficult to store. The person in the gross room ends up placing all remaining tissue in one of our regular specimen bottles after completing the gross examination. I am going to tell them that we are not going to accept the specimens unless you can convince me that it is O.K. (and I don't think that is going to happen). Thanks for your time. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Thu Jul 17 18:15:17 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Jul 17 18:15:32 2008 Subject: [Histonet] hcv and mastcells In-Reply-To: <5EB233BCA05B4CE0A1336756E4C4B44D@dielangs.at> Message-ID: Gudrun, Teri Johnson [TJJ@Stowers-Institute.org]gave an excellent summary of this problem on the IHC resource groups listserver (Wednesday, 21 May 2008) and hopefully with her leave, I have reproduced it below: "I did some rooting around in the literature and found out why you get antibody staining (in negative control material) of mast cells, and what you can do about it. Summary During investigations of murine and human mast cell immunoreactivity with potential anti-interleukin-4 antibodies, non-specific, non-immunological labelling of mouse and human mast cells became apparent. Non-specific, non-immunological labelling was identified by (i) immunolabelling of mast cells when using control isotype primary antibodies, (ii) ability of conjugated secondary antibodies to label mast cells without prior mast cell exposure to a primary antibody, (iii) extinction of the non-specific labelling and retention of specific labelling when the pH of the diluting and washing buffers is shifted from pH 7.2 to pH 6.0, and (iv) reduction/extinction of the labelling when the antibodies are pre-incubated with soluble heparin prior to immunostaining. The site of the reactivity on the electron microscope level was shown to be confined to the mast cell secretory granules. The results of this study support the hypothesis that non-specific labelling of mast cells results from an ionic interaction between the F(ab)2 segments of antibodies and the heparin constituent of the mast cell secretory granules. This study points out the necessity of stringent controls when using immunohistochemistry to determine mast cell reactivity to various antibodies. (Ref: Histochemical Journal, Vol. 25(9), Sept 1993, Schiltz et al) An additional source for this (1989-1990 publication): It is postulated that the granules of TMC bind certain antibodies by a cation-exchange mechanism involving ionic interactions with positively charged groups in the F(ab')2 and/or Fc segments. (J Histochem Cytochem 38:859-867, 1990) And, finally, I found this little gem: To avoid nonspecific binding of the antibodies to the heparin contained in mast cells, the specificity of immunostaining was checked. PBS for washing and dilution of the antibodies was acidified to pH 6.0. Preadsorption of the STG I antibody with 1000 U/ml heparin (Sigma; St Louis, MO) for 60 min at RT before use was carried out to avoid nonspecific binding of immunoglobulins and heparin. The specificity of the immunohistochemistry was confirmed with negative controls: absence of primary or secondary antibody and avidin-labeled peroxidase. Normal non-immune mouse serum was also used instead of the primary antibody. (Ref: JHistochemCytochem 49(3): 341, Kumura et al) Tammy: You never mentioned if you were getting mast cell staining in your negative control. If no, then the above may not pertain to you and you may have an issue of specific cross-reactivity with the antibody." Good luck! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 Thanks Terri, Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Friday, 18 July 2008 2:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] hcv and mastcells Hi all! I did an immunohistochemistry for heptatitis C virus on skin of a man, who formerly had an hcv infection. The mastcells showed a granular staining in the cytoplasm. Has anyone experience or explanations with this phenomen? Could it be due to a cross-reaction or are there really viruses (at least particel) in the mastcell-granula? Thanks in advance Gudrun Lang ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From Rcartun <@t> harthosp.org Fri Jul 18 08:42:30 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Jul 18 08:42:43 2008 Subject: [Histonet] ER,PR, HER2/Neu In-Reply-To: <29BE166A2CF48D459853F8EC57CD37E8011D8956@EXCHANGECLUSTER.yumaregional.local> References: <5EB233BCA05B4CE0A1336756E4C4B44D@dielangs.at> <29BE166A2CF48D459853F8EC57CD37E8011D8956@EXCHANGECLUSTER.yumaregional.local> Message-ID: <4880658602000077000042F7@gwmail6.harthosp.org> Why do you want to use image analysis? Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Jesus Ellin" 07/17/08 12:39 PM >>> We are in the early stages of getting into ER/PR/HER2Neu scanning technology using the Ventana Image Analysis System (VIAS). Wanting to know the Pit falls and also what else to expect when going into this realm,, Any information would greatly be appreciated. Jesus Ellin HT/PA ASCP Yuma Regional Medical Center 928-336-1743 This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Loralee_Mcmahon <@t> URMC.Rochester.edu Fri Jul 18 08:50:05 2008 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Fri Jul 18 08:52:22 2008 Subject: [Histonet] ER,PR, HER2/Neu References: <5EB233BCA05B4CE0A1336756E4C4B44D@dielangs.at><29BE166A2CF48D459853F8EC57CD37E8011D8956@EXCHANGECLUSTER.yumaregional.local> <4880658602000077000042F7@gwmail6.harthosp.org> Message-ID: <2CF6F6B05263EA4EBAB07781B51E5DB0025A2810@e2k3ms1.urmc-sh.rochester.edu> We are in the process of purchasing a image analysis machine from DAKO called the ACIS. I believe that the ACIS has a nicer interface with the user, but I haven't had the opportunity to actually sit down and use the Ventana machine myself. I have only seen it being used. Loralee McMahon, HTL (ASCP) ICC Supervisor University of Rochester Department of Pathology (585) 275-7210 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Richard Cartun Sent: Fri 7/18/2008 9:42 AM To: histonet@lists.utsouthwestern.edu; Jesus Ellin Subject: Re: [Histonet] ER,PR, HER2/Neu Why do you want to use image analysis? Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Jesus Ellin" 07/17/08 12:39 PM >>> We are in the early stages of getting into ER/PR/HER2Neu scanning technology using the Ventana Image Analysis System (VIAS). Wanting to know the Pit falls and also what else to expect when going into this realm,, Any information would greatly be appreciated. Jesus Ellin HT/PA ASCP Yuma Regional Medical Center 928-336-1743 This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gvdobbin <@t> ihis.org Fri Jul 18 09:24:47 2008 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Fri Jul 18 09:25:10 2008 Subject: [Histonet] IBF and prostates-your experience Message-ID: Hi folks, If were to use IBF for the collection of prostate cores and they only fixed in IBF for 3 hrs before being transferred to 10% formalin for processing with everything else, are the benefits of better nuclear morphology still likely to be realized? Thank you. Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "Success is not the key to happiness. Happiness is the key to success. If you love what you are doing, you will be successful." - Albert Schweitzer ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- From JEllin <@t> yumaregional.org Fri Jul 18 09:51:14 2008 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Fri Jul 18 09:51:20 2008 Subject: [Histonet] ER,PR, HER2/Neu In-Reply-To: <4880658602000077000042F7@gwmail6.harthosp.org> Message-ID: <29BE166A2CF48D459853F8EC57CD37E8011D8960@EXCHANGECLUSTER.yumaregional.local> We will be doing in-house ER,PR, Her/Neu studies and report out results for treatment, instead of sending out to reference labs for this service, We are also looking out intergrating this platform with our Pathology Information System, so that pathologist can use this tool without having to go out of the current program,, early stages with this development though. Jesus Ellin HT/PA ASCP Yuma Regional Medical Center 928-336-1743 This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. From gvdobbin <@t> ihis.org Fri Jul 18 09:51:24 2008 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Fri Jul 18 09:51:56 2008 Subject: [Histonet] IBF and prostates-your experience Message-ID: Hi Richard, I always assume most people in Histology know more than I do, so I figured IBF was well known in histo circles. Sorry. IBF is a commecial fixative available from Surgipath, which contains isopropyl alcohol, methanol, barium chloride, and low percent formalin (<3% by weight). Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "Success is not the key to happiness. Happiness is the key to success. If you love what you are doing, you will be successful." - Albert Schweitzer >>> "Richard Cartun" 7/18/2008 11:39 AM >>> Hi Greg: What is "IBF"? Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Greg Dobbin" 07/18/08 10:24 AM >>> Hi folks, If were to use IBF for the collection of prostate cores and they only fixed in IBF for 3 hrs before being transferred to 10% formalin for processing with everything else, are the benefits of better nuclear morphology still likely to be realized? Thank you. Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "Success is not the key to happiness. Happiness is the key to success. If you love what you are doing, you will be successful." - Albert Schweitzer ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- From rjbuesa <@t> yahoo.com Fri Jul 18 10:08:02 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jul 18 10:08:07 2008 Subject: [Histonet] IBF and prostates-your experience In-Reply-To: Message-ID: <580192.32789.qm@web65708.mail.ac4.yahoo.com> Probably, because in 3 hours in IBF the biopsies will probably be fixed with the alcohols contained in IBF (methanol and 2-propanol). What I don't see is why you want to transfer?them ?to 10% NBF. Ren? J. --- On Fri, 7/18/08, Greg Dobbin wrote: From: Greg Dobbin Subject: [Histonet] IBF and prostates-your experience To: Histonet@lists.utsouthwestern.edu Date: Friday, July 18, 2008, 10:24 AM Hi folks, If were to use IBF for the collection of prostate cores and they only fixed in IBF for 3 hrs before being transferred to 10% formalin for processing with everything else, are the benefits of better nuclear morphology still likely to be realized? Thank you. Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "Success is not the key to happiness. Happiness is the key to success. If you love what you are doing, you will be successful." - Albert Schweitzer ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Herrick.James <@t> mayo.edu Fri Jul 18 11:50:18 2008 From: Herrick.James <@t> mayo.edu (Herrick, James L.) Date: Fri Jul 18 11:50:22 2008 Subject: FW: [Histonet] Question on Aluminum Staining Message-ID: -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Herrick, James L. Sent: Thursday, July 17, 2008 3:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question on Aluminum Staining Hi everyone, We are having trouble running ASA (acid solochrome azurin) stains on human iliac crest bone biopsies for the detection of aluminum deposits. Our negatives are showing a lot of blue around the outer edges of the specimen. Although we cannot see staining around the trabecular bone, we should also not see it around the periphery of the bone specimen. The positive, on the other hand, looks very good. Has anyone had experience with this type of stain, and if so, would you happen to know what we may be doing wrong? The recipe we use for our stain is .2 gms. of Chromeazural B in 20mL of dH2O with a pH @ 5.0 (We incubate at room temp for 18hrs.), destain with 95% methanol, rinse in dH2O, dehydrate, clear and coverslip. Also, another problem we have encountered in the past, is a gold discoloring of the specimen. Have you seen this problem before, and if so, do you know how to correct it? Any help we can get with this dilemma is very much appreciated. Thanks again, Jim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jason.Burrill <@t> crl.com Fri Jul 18 12:08:20 2008 From: Jason.Burrill <@t> crl.com (Burrill, Jason) Date: Fri Jul 18 12:08:33 2008 Subject: [Histonet] RE: Formalin Containers In-Reply-To: References: Message-ID: <1AD4E907E9B6F648AEF1B3A20A9B0E1E013B8D93@shr-exch2.na01.crl.com> We actually prefer using the bags and suggest our customers ship their samples in them. Although it does create a little more work with cutting them open they are a lot less likely to leak during transit and we find it easier to store the samples. Jason Burrill Sr. Manager, Histology and Laboratory Safety Research Animal Diagnostic Services Charles River 251 Ballardvale St Wilmington, MA 01887 Office: 781-222-6152 Fax: 978-988-8793 jason.burrill@crl.com www.criver.com Accelerating Drug Development. Exactly. Notice - This email and any files transmitted with it are confidential and may contain privileged and/or proprietary information. You must not disclose this message to another party without Charles River's express written consent. If you are not the intended recipient you must not copy, distribute or use this email or the information contained in it for any purpose other than to notify us. If you have received this message in error, please notify Charles River immediately, and delete it from your system. From Pkarlisch <@t> hmc.psu.edu Fri Jul 18 12:34:46 2008 From: Pkarlisch <@t> hmc.psu.edu (Patricia Karlisch) Date: Fri Jul 18 12:35:31 2008 Subject: [Histonet] Formalin in plastic bags Message-ID: <48809BF6.07F7.008C.0@hmc.psu.edu> Richard, There are standards for sending specimens that are in a hazardous solution, like Formalin. Plastic bags are secondary containers and should not be used to transport fluid. The specimens should be in a secure plastic container that seals tightly and will not puncture causing injury to others. Pat Karlisch Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. From Todd.Angelucci <@t> leica-microsystems.com Fri Jul 18 12:50:25 2008 From: Todd.Angelucci <@t> leica-microsystems.com (Todd.Angelucci@leica-microsystems.com) Date: Fri Jul 18 12:50:34 2008 Subject: [Histonet] Re: Histonet Digest, Vol 56, Issue 21 Message-ID: Todd Angelucci IHC Sales Sales Specialist Cell Phone: 203-209-5399 ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From RSRICHMOND <@t> aol.com Fri Jul 18 12:51:00 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Fri Jul 18 12:51:04 2008 Subject: [Histonet] Re: IBF and prostates-your experience Message-ID: Greg Dobbin at Charlottetown PE asks >>If I were to use IBF for the collection of prostate cores and they only fixed in IBF for 3 hrs before being transferred to 10% formalin for processing with everything else, are the benefits of better nuclear morphology still likely to be realized?<< Greg goes on to describe Surgipath's IBF as containing alcohols, barium chloride, and a smaller amount of formaldehyde. I think this is an extremely bad idea. The histopathological standards for diagnosing prostate cancer depend on neutral buffered formalin fixation. NBF fixation demonstrates nucleoli only in tumor cells, while other fixatives can demonstrate nucleoli in benign prostate cells also. I don't see any benefit in the off-brand fixation, and it can lead an unaware pathologist into a disastrous overdiagnosis. Bob Richmond Samurai Pathologist Knoxville TN From Janet.Bonner <@t> FLHOSP.ORG Fri Jul 18 12:58:21 2008 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Jul 18 12:58:37 2008 Subject: [Histonet] Formalin in plastic bags References: <48809BF6.07F7.008C.0@hmc.psu.edu> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2713@fhosxchmb006.ADVENTISTCORP.NET> Dear All, To transport specimens from one floor to another or from facility to facility, that are submitted in a plastic screw-top container, is it a requirement (CAP or otherwise), to also have it in a secondary container - like a biohazard bag? Would it be acceptable to transport the plastic container directly in a thermoTOTE with other specimen containers from different patients, with the requisitions in an outside pocket? Janet L. Bonner, HTL (ASCP) Pathology Laboratory Florida Hospital Winter Park janet.bonner@FLHOSP.org 407-646-7559 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Patricia Karlisch Sent: Fri 7/18/2008 1:34 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin in plastic bags Richard, There are standards for sending specimens that are in a hazardous solution, like Formalin. Plastic bags are secondary containers and should not be used to transport fluid. The specimens should be in a secure plastic container that seals tightly and will not puncture causing injury to others. Pat Karlisch ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From slappycraw <@t> yahoo.com Fri Jul 18 13:05:10 2008 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Fri Jul 18 13:05:16 2008 Subject: [Histonet] Posting for a friend Message-ID: <556743.98541.qm@web53604.mail.re2.yahoo.com> Looking for a job in the great state of Montana for a friend that has 25 years experience in clinical and research histology/immunohistochemistry. Anyone out there in histonetland know of any? Thanks. Larry A. Woody Seattle, Wa. From Tbarnhart <@t> primecare.org Fri Jul 18 13:07:02 2008 From: Tbarnhart <@t> primecare.org (Barnhart, Tammy) Date: Fri Jul 18 13:06:21 2008 Subject: [Histonet] RE: Formalin Containers Message-ID: <4F0B7161A6CD524FAD8017D52E1553407AE775@exchangent> When I started my current position, I had no experience with whirl-top specimen bags. In fact, my notion was that they would leak and not work nearly as good as screw top containers. We get specimens mailed to us from all over the Dakotas and also send our specimens through our pneumatic tube system. The screw top container leak, the whirl-top bags do not. I much prefer using the bags. We don't need to cut them open, so that is not a problem for us. Tammy Barnhart, BS, HTL(ASCP) Anatomic Pathology Supervisor St. Alexius Medical Center 900 E. Broadway Bismarck, ND 58501 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Burrill, Jason Sent: Friday, July 18, 2008 12:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Formalin Containers We actually prefer using the bags and suggest our customers ship their samples in them. Although it does create a little more work with cutting them open they are a lot less likely to leak during transit and we find it easier to store the samples. Jason Burrill Sr. Manager, Histology and Laboratory Safety Research Animal Diagnostic Services Charles River 251 Ballardvale St Wilmington, MA 01887 Office: 781-222-6152 Fax: 978-988-8793 jason.burrill@crl.com www.criver.com Accelerating Drug Development. Exactly. Notice - This email and any files transmitted with it are confidential and may contain privileged and/or proprietary information. You must not disclose this message to another party without Charles River's express written consent. If you are not the intended recipient you must not copy, distribute or use this email or the information contained in it for any purpose other than to notify us. If you have received this message in error, please notify Charles River immediately, and delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Pkarlisch <@t> hmc.psu.edu Fri Jul 18 14:02:34 2008 From: Pkarlisch <@t> hmc.psu.edu (Patricia Karlisch) Date: Fri Jul 18 14:03:07 2008 Subject: [Histonet] Formalin in plastic bags In-Reply-To: <5F31F38C96781A4FBE3196EBC22D47807F2713@fhosxchmb006.ADVENTISTCORP.NET> References: <48809BF6.07F7.008C.0@hmc.psu.edu> <5F31F38C96781A4FBE3196EBC22D47807F2713@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: <4880B08A.07F7.008C.0@hmc.psu.edu> Janet, Specimens must be transported in a secondary container and this should NOT be a paper bag. This can be a Thermo tote or a styrofoam box that can be secured tightly if there is a spill. Market lab makes numerous secondary containers. The secondary container must be clearly marked Biohazardous material if it is carrying such items. Pat Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. >>> "Bonner, Janet" 7/18/2008 1:58 PM >>> Dear All, To transport specimens from one floor to another or from facility to facility, that are submitted in a plastic screw-top container, is it a requirement (CAP or otherwise), to also have it in a secondary container - like a biohazard bag? Would it be acceptable to transport the plastic container directly in a thermoTOTE with other specimen containers from different patients, with the requisitions in an outside pocket? Janet L. Bonner, HTL (ASCP) Pathology Laboratory Florida Hospital Winter Park janet.bonner@FLHOSP.org 407-646-7559 From: histonet-bounces@lists.utsouthwestern.edu on behalf of Patricia Karlisch Sent: Fri 7/18/2008 1:34 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin in plastic bags Richard, There are standards for sending specimens that are in a hazardous solution, like Formalin. Plastic bags are secondary containers and should not be used to transport fluid. The specimens should be in a secure plastic container that seals tightly and will not puncture causing injury to others. Pat Karlisch ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From estellamireles <@t> gmail.com Fri Jul 18 14:39:41 2008 From: estellamireles <@t> gmail.com (Estella Mireles) Date: Fri Jul 18 14:39:46 2008 Subject: [Histonet] n Gieson's picric fuchsin Message-ID: I am searching for the written procedure for the Stevenel blue/Van Gieson's picric fuchsin. Would really appreciate the help. Thanks From thisisann <@t> aol.com Fri Jul 18 14:51:12 2008 From: thisisann <@t> aol.com (thisisann@aol.com) Date: Fri Jul 18 14:51:36 2008 Subject: [Histonet] Control Tissue Message-ID: <8CAB726C41F6F80-11FC-1998@WEBMAIL-MB13.sysops.aol.com> I am in need of umbilical cord to use for an Alcian blue control.? Can anyone spare a block or two? Ann Angelo QDx Pathology Services From gayle.callis <@t> bresnan.net Fri Jul 18 15:12:43 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Fri Jul 18 15:12:47 2008 Subject: [Histonet] n Gieson's picric fuchsin References: Message-ID: <003601c8e912$a4f7de50$6501a8c0@DHXTS541> I will attach a copy to you. To avoid having to make up this staining solution, buy Sandersons Bone Stain from Surgipath. It is the same stain, only she knew how to simplfy and improve making up this very tedious, hard to prepare staining solution. The stain is not stable, as it the potassium permanganate continues to oxidize the methylene blue over time and with exposure to heat. Also, try to access the original publication for bone staining. Gayle M. Callis HTL/HT/MT(ASCP0 Bozeman MT ----- Original Message ----- From: "Estella Mireles" To: Sent: Friday, July 18, 2008 1:39 PM Subject: [Histonet] n Gieson's picric fuchsin >I am searching for the written procedure for the Stevenel blue/Van Gieson's > picric fuchsin. > > Would really appreciate the help. > > Thanks > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pierre.chaumat <@t> alphelys.com Fri Jul 18 16:08:59 2008 From: pierre.chaumat <@t> alphelys.com (Pierre CHAUMAT) Date: Fri Jul 18 16:09:10 2008 Subject: [Histonet] 1. LCM on decaled bone sections (Elizabeth M Heimrich In-Reply-To: <0MKqlY-1KJtKk39Gy-0005Aa@mx.kundenserver.de> Message-ID: <0MKxQS-1KJxCF1NrE-0001yX@mrelayeu.kundenserver.de> Dear Elisabeth, I think we have some starts of answers for you. We are a french company that does promote and sell LCM system from Arcturus since 99 and we have developped a strong expertise for that. Also, as a matter of coincidence for the discussion, we have developped a formalin free fixative marketed under the name of RCL2. Multiple things I can say : 1- Formalin fixation will most probably annhilate your possibilities of working with proper RNA and proteins far beyond decal 2- Working with frozen section does not leave you a good enought morphology to work with when selecting specific cells during LCM I would also encourage you to read some publications about RCL2 having demonstrated its performance including preserving both morphology and molecular content at a very high level. RCL2 fixed specimens can be paraffin embedded and then stored at room temp. J of Molecular Diagnostics. 2006 Vol.8 p.157-169 Delfour C - Boulle N.pdf If you need more details, please don't hesitate to contact me again whether it is LCM protocols or of course RCL2 use...! Best regards Pierre Pierre CHAUMAT President & CEO ALPHELYS SAS Passage Paul Langevin Ferme des Ebisoires 78370 PLAISIR, FRANCE Tel +33 1 30 07 52 95 Fax +33 1 30 07 51 56 Cell +33 6 03 47 75 92 http://www.alphelys.com -----Message d'origine----- De : histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] De la part de histonet-request@lists.utsouthwestern.edu Envoy? : vendredi 18 juillet 2008 19:02 ? : histonet@lists.utsouthwestern.edu Objet : Histonet Digest, Vol 56, Issue 21 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. LCM on decaled bone sections (Elizabeth M Heimrich) 2. Re: borrelia burgdorferi protocol (Richard Cartun) 3. AW: [Histonet] borrelia burgdorferi protocol (Gudrun Lang) 4. AP Cerner Copath vs Sunquest Copath vs others (Cristian Britos) 5. Question on Aluminum Staining (Herrick, James L.) 6. Re: CD4 Fluorescent Staining (Gayle Callis) 7. Formlain containers (Richard Cartun) 8. Desmoglein 3 antibody (Patti Loykasek) 9. RE: Formlain containers (Martin, Gary) 10. RE: hcv and mastcells (Tony Henwood) 11. Re: ER,PR, HER2/Neu (Richard Cartun) 12. RE: ER,PR, HER2/Neu (McMahon, Loralee A) 13. IBF and prostates-your experience (Greg Dobbin) 14. RE: ER,PR, HER2/Neu (Jesus Ellin) 15. Re: IBF and prostates-your experience (Greg Dobbin) 16. Re: IBF and prostates-your experience (Rene J Buesa) 17. FW: [Histonet] Question on Aluminum Staining (Herrick, James L.) ---------------------------------------------------------------------- Message: 1 Date: Thu, 17 Jul 2008 14:01:16 -0400 From: Elizabeth M Heimrich Subject: [Histonet] LCM on decaled bone sections To: histonet@lists.utsouthwestern.edu Message-ID: <487F88EC.8010505@bms.com> Content-Type: text/plain; charset="iso-8859-1" Hello everyone, I have a question regarding the doability of LCM on decaled rat joint specimens and the subsequent RNA extraction and amplification. The rat joints have not been harvested yet, so there is no fixative decided upon. Is this a feasible procedure? Does the decalcification of the bone destroy the RNA, or is the main issue the formaldehyde cross-linking the proteins if using FFPE tissue. I have queried a few scientists around here and have received conflicting answers, so I come to the net....hoping to resolve this issue. Some say to dissect out the synovium and take cryo sections because the Formaldehyde will interfere with the RNA signal. Others say that the problem resides with the decal procedure itself. Any and all thoughts welcome. Thank you, Beth ------------------------------ Message: 2 Date: Thu, 17 Jul 2008 15:00:19 -0400 From: "Richard Cartun" Subject: Re: [Histonet] borrelia burgdorferi protocol To: , Message-ID: <487F5E8302000077000042AD@gwmail6.harthosp.org> Content-Type: text/plain; charset=US-ASCII I have been doing IHC for Borrelia burgdorferi for years and have never seen a positive human case; only in animal tissue. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Gudrun Lang" 07/17/08 12:33 PM >>> Hi all! Can anyone provide me a borrelia burgdorferi protocol for ihc, preferable on the Benchmark XT? We will purchase a polyclonal antibody from Acris. In the datasheet is no hint about pretreatment or titer. So some information would be helpful to start with. Thanks in advance Gudrun Lang ------------------------------ Message: 3 Date: Thu, 17 Jul 2008 21:09:26 +0200 From: "Gudrun Lang" Subject: AW: [Histonet] borrelia burgdorferi protocol To: "'Richard Cartun'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <37E7D6EF2B8D440DB16B613E1E6D7A87@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" In spite of your "negative experiences" would you please share your protocol with me? And what do you think is the cause, why it doesn't work on human tissue? Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr|ngliche Nachricht----- Von: Richard Cartun [mailto:Rcartun@harthosp.org] Gesendet: Donnerstag, 17. Juli 2008 21:00 An: gu.lang@gmx.at; histonet@lists.utsouthwestern.edu Betreff: Re: [Histonet] borrelia burgdorferi protocol I have been doing IHC for Borrelia burgdorferi for years and have never seen a positive human case; only in animal tissue. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Gudrun Lang" 07/17/08 12:33 PM >>> Hi all! Can anyone provide me a borrelia burgdorferi protocol for ihc, preferable on the Benchmark XT? We will purchase a polyclonal antibody from Acris. In the datasheet is no hint about pretreatment or titer. So some information would be helpful to start with. Thanks in advance Gudrun Lang ------------------------------ Message: 4 Date: Thu, 17 Jul 2008 15:26:02 -0400 From: "Cristian Britos" Subject: [Histonet] AP Cerner Copath vs Sunquest Copath vs others To: "Richard Cartun" , , Message-ID: Content-Type: text/plain; charset="us-ascii" Would someone be willing to share their experiences about Sunquest copath as their LIS system for pathology versus other systems? Needless to say, we are looking for options.... (currently we have cerner copath 3.0) Thanks, Cris ------------------------------ Message: 5 Date: Thu, 17 Jul 2008 15:16:09 -0500 From: "Herrick, James L." Subject: [Histonet] Question on Aluminum Staining To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi everyone, We are having trouble running ASA (acid solochrome azurin) stains on human iliac crest bone biopsies for the detection of aluminum deposits. Our negatives are showing a lot of blue around the outer edges of the specimen. Although we cannot see staining around the trabecular bone, we should also not see it around the periphery of the bone specimen. The positive, on the other hand, looks very good. Has anyone had experience with this type of stain, and if so, would you happen to know what we may be doing wrong? The recipe we use for our stain is .2 gms. of Chromeazural B in 20mL of dH2O with a pH @ 5.0 (We incubate at room temp for 18hrs.), destain with 95% methanol, rinse in dH2O, dehydrate, clear and coverslip. Also, another problem we have encountered in the past, is a gold discoloring of the specimen. Have you seen this problem before, and if so, do you know how to correct it? Any help we can get with this dilemma is very much appreciated. Thanks again, Jim ------------------------------ Message: 6 Date: Thu, 17 Jul 2008 14:41:37 -0600 From: "Gayle Callis" Subject: Re: [Histonet] CD4 Fluorescent Staining To: "McMahon, Loralee A" , Message-ID: <006801c8e84d$844c5520$6401a8c0@DHXTS541> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Just to clarify exactly what you are doing - are you using a rat antiMouse CD4 conjugated to Alexa 488? After I get the answer, I will elaborate. We do successful CD4 immunofluorescent staining but never use a CD4 primary direct, with the primary conjugated to the fluorphore. We have four different ways of doing the staining. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT ----- Original Message ----- From: "McMahon, Loralee A" To: Sent: Thursday, July 17, 2008 9:38 AM Subject: [Histonet] CD4 Fluorescent Staining I am trying an antibody called Alexa Fluor 488 anti-mouse CD4 on fresh frozen mouse tissue. Having only worked with AED/DAB staining, not very familiar with this. Does anyone have any experience with this and if you could send me some tips/protocols. Thanks ahead of time Loralee McMahon, HTL (ASCP) ICC Supervisor University of Rochester Department of Pathology (585) 275-7210 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Thu, 17 Jul 2008 17:39:27 -0400 From: "Richard Cartun" Subject: [Histonet] Formlain containers To: "Histonet" Message-ID: <487F83CF02000077000042C8@gwmail6.harthosp.org> Content-Type: text/plain; charset=US-ASCII Does anyone receive tissue specimens in plastic bags containing formalin? We do and I don't like it. For some reason this client likes to use these plastic bags instead of our standard formalin bottles. The bags have to be cut open and are difficult to store. The person in the gross room ends up placing all remaining tissue in one of our regular specimen bottles after completing the gross examination. I am going to tell them that we are not going to accept the specimens unless you can convince me that it is O.K. (and I don't think that is going to happen). Thanks for your time. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax ------------------------------ Message: 8 Date: Thu, 17 Jul 2008 14:48:18 -0700 From: Patti Loykasek Subject: [Histonet] Desmoglein 3 antibody To: histonet Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi all. We have been trying to work up an antibody, desmoglein 3, in squamous cell lung carcinoma. This is based on an article in Feb. 2007 Human Pathology. We have had great difficulty in optimizing the antibody in FFPE. If anyone else has this antibody up & working well, I would appreciate any help with it. Thanks. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ------------------------------ Message: 9 Date: Thu, 17 Jul 2008 15:59:36 -0700 From: "Martin, Gary" Subject: RE: [Histonet] Formlain containers To: "Richard Cartun" , "Histonet" Message-ID: <6ED9D4252F278841A0593D3D788AF24C02DFF0D2@mailsvr.MARSHMED.local> Content-Type: text/plain; charset="us-ascii" We use both ... the bags have not been a problem ... as a matter for fact I prefer them. They seem to store easier. We also noticed that many times clients try to stuff a very large specimen into a small bottle, and fixation can suffer. The bags we use are designed for pathology use. They are manufactured by Bitran, and sold by Cardinal Health Gary -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, July 17, 2008 2:39 PM To: Histonet Subject: [Histonet] Formlain containers Does anyone receive tissue specimens in plastic bags containing formalin? We do and I don't like it. For some reason this client likes to use these plastic bags instead of our standard formalin bottles. The bags have to be cut open and are difficult to store. The person in the gross room ends up placing all remaining tissue in one of our regular specimen bottles after completing the gross examination. I am going to tell them that we are not going to accept the specimens unless you can convince me that it is O.K. (and I don't think that is going to happen). Thanks for your time. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Fri, 18 Jul 2008 09:15:17 +1000 From: "Tony Henwood" Subject: RE: [Histonet] hcv and mastcells To: , Cc: TJJ@Stowers-Institute.org Message-ID: Content-Type: text/plain; charset="us-ascii" Gudrun, Teri Johnson [TJJ@Stowers-Institute.org]gave an excellent summary of this problem on the IHC resource groups listserver (Wednesday, 21 May 2008) and hopefully with her leave, I have reproduced it below: "I did some rooting around in the literature and found out why you get antibody staining (in negative control material) of mast cells, and what you can do about it. Summary During investigations of murine and human mast cell immunoreactivity with potential anti-interleukin-4 antibodies, non-specific, non-immunological labelling of mouse and human mast cells became apparent. Non-specific, non-immunological labelling was identified by (i) immunolabelling of mast cells when using control isotype primary antibodies, (ii) ability of conjugated secondary antibodies to label mast cells without prior mast cell exposure to a primary antibody, (iii) extinction of the non-specific labelling and retention of specific labelling when the pH of the diluting and washing buffers is shifted from pH 7.2 to pH 6.0, and (iv) reduction/extinction of the labelling when the antibodies are pre-incubated with soluble heparin prior to immunostaining. The site of the reactivity on the electron microscope level was shown to be confined to the mast cell secretory granules. The results of this study support the hypothesis that non-specific labelling of mast cells results from an ionic interaction between the F(ab)2 segments of antibodies and the heparin constituent of the mast cell secretory granules. This study points out the necessity of stringent controls when using immunohistochemistry to determine mast cell reactivity to various antibodies. (Ref: Histochemical Journal, Vol. 25(9), Sept 1993, Schiltz et al) An additional source for this (1989-1990 publication): It is postulated that the granules of TMC bind certain antibodies by a cation-exchange mechanism involving ionic interactions with positively charged groups in the F(ab')2 and/or Fc segments. (J Histochem Cytochem 38:859-867, 1990) And, finally, I found this little gem: To avoid nonspecific binding of the antibodies to the heparin contained in mast cells, the specificity of immunostaining was checked. PBS for washing and dilution of the antibodies was acidified to pH 6.0. Preadsorption of the STG I antibody with 1000 U/ml heparin (Sigma; St Louis, MO) for 60 min at RT before use was carried out to avoid nonspecific binding of immunoglobulins and heparin. The specificity of the immunohistochemistry was confirmed with negative controls: absence of primary or secondary antibody and avidin-labeled peroxidase. Normal non-immune mouse serum was also used instead of the primary antibody. (Ref: JHistochemCytochem 49(3): 341, Kumura et al) Tammy: You never mentioned if you were getting mast cell staining in your negative control. If no, then the above may not pertain to you and you may have an issue of specific cross-reactivity with the antibody." Good luck! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 Thanks Terri, Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Friday, 18 July 2008 2:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] hcv and mastcells Hi all! I did an immunohistochemistry for heptatitis C virus on skin of a man, who formerly had an hcv infection. The mastcells showed a granular staining in the cytoplasm. Has anyone experience or explanations with this phenomen? Could it be due to a cross-reaction or are there really viruses (at least particel) in the mastcell-granula? Thanks in advance Gudrun Lang ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 11 Date: Fri, 18 Jul 2008 09:42:30 -0400 From: "Richard Cartun" Subject: Re: [Histonet] ER,PR, HER2/Neu To: , "Jesus Ellin" Message-ID: <4880658602000077000042F7@gwmail6.harthosp.org> Content-Type: text/plain; charset=US-ASCII Why do you want to use image analysis? Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Jesus Ellin" 07/17/08 12:39 PM >>> We are in the early stages of getting into ER/PR/HER2Neu scanning technology using the Ventana Image Analysis System (VIAS). Wanting to know the Pit falls and also what else to expect when going into this realm,, Any information would greatly be appreciated. Jesus Ellin HT/PA ASCP Yuma Regional Medical Center 928-336-1743 This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Fri, 18 Jul 2008 09:50:05 -0400 From: "McMahon, Loralee A" Subject: RE: [Histonet] ER,PR, HER2/Neu To: "Richard Cartun" , , "Jesus Ellin" Message-ID: <2CF6F6B05263EA4EBAB07781B51E5DB0025A2810@e2k3ms1.urmc-sh.rochester.edu> Content-Type: text/plain; charset="iso-8859-1" We are in the process of purchasing a image analysis machine from DAKO called the ACIS. I believe that the ACIS has a nicer interface with the user, but I haven't had the opportunity to actually sit down and use the Ventana machine myself. I have only seen it being used. Loralee McMahon, HTL (ASCP) ICC Supervisor University of Rochester Department of Pathology (585) 275-7210 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Richard Cartun Sent: Fri 7/18/2008 9:42 AM To: histonet@lists.utsouthwestern.edu; Jesus Ellin Subject: Re: [Histonet] ER,PR, HER2/Neu Why do you want to use image analysis? Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Jesus Ellin" 07/17/08 12:39 PM >>> We are in the early stages of getting into ER/PR/HER2Neu scanning technology using the Ventana Image Analysis System (VIAS). Wanting to know the Pit falls and also what else to expect when going into this realm,, Any information would greatly be appreciated. Jesus Ellin HT/PA ASCP Yuma Regional Medical Center 928-336-1743 This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Fri, 18 Jul 2008 11:24:47 -0300 From: "Greg Dobbin" Subject: [Histonet] IBF and prostates-your experience To: Message-ID: Content-Type: text/plain; charset=US-ASCII Hi folks, If were to use IBF for the collection of prostate cores and they only fixed in IBF for 3 hrs before being transferred to 10% formalin for processing with everything else, are the benefits of better nuclear morphology still likely to be realized? Thank you. Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "Success is not the key to happiness. Happiness is the key to success. If you love what you are doing, you will be successful." - Albert Schweitzer ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- ------------------------------ Message: 14 Date: Fri, 18 Jul 2008 07:51:14 -0700 From: "Jesus Ellin" Subject: RE: [Histonet] ER,PR, HER2/Neu To: "Richard Cartun" , Message-ID: <29BE166A2CF48D459853F8EC57CD37E8011D8960@EXCHANGECLUSTER.yumaregional.local > Content-Type: text/plain; charset="US-ASCII" We will be doing in-house ER,PR, Her/Neu studies and report out results for treatment, instead of sending out to reference labs for this service, We are also looking out intergrating this platform with our Pathology Information System, so that pathologist can use this tool without having to go out of the current program,, early stages with this development though. Jesus Ellin HT/PA ASCP Yuma Regional Medical Center 928-336-1743 This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ------------------------------ Message: 15 Date: Fri, 18 Jul 2008 11:51:24 -0300 From: "Greg Dobbin" Subject: Re: [Histonet] IBF and prostates-your experience To: , Message-ID: Content-Type: text/plain; charset=US-ASCII Hi Richard, I always assume most people in Histology know more than I do, so I figured IBF was well known in histo circles. Sorry. IBF is a commecial fixative available from Surgipath, which contains isopropyl alcohol, methanol, barium chloride, and low percent formalin (<3% by weight). Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "Success is not the key to happiness. Happiness is the key to success. If you love what you are doing, you will be successful." - Albert Schweitzer >>> "Richard Cartun" 7/18/2008 11:39 AM >>> Hi Greg: What is "IBF"? Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Greg Dobbin" 07/18/08 10:24 AM >>> Hi folks, If were to use IBF for the collection of prostate cores and they only fixed in IBF for 3 hrs before being transferred to 10% formalin for processing with everything else, are the benefits of better nuclear morphology still likely to be realized? Thank you. Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "Success is not the key to happiness. Happiness is the key to success. If you love what you are doing, you will be successful." - Albert Schweitzer ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- ------------------------------ Message: 16 Date: Fri, 18 Jul 2008 08:08:02 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] IBF and prostates-your experience To: Histonet@lists.utsouthwestern.edu, Greg Dobbin Message-ID: <580192.32789.qm@web65708.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Probably, because in 3 hours in IBF the biopsies will probably be fixed with the alcohols contained in IBF (methanol and 2-propanol). What I don't see is why you want to transfer them to 10% NBF. Reni J. --- On Fri, 7/18/08, Greg Dobbin wrote: From: Greg Dobbin Subject: [Histonet] IBF and prostates-your experience To: Histonet@lists.utsouthwestern.edu Date: Friday, July 18, 2008, 10:24 AM Hi folks, If were to use IBF for the collection of prostate cores and they only fixed in IBF for 3 hrs before being transferred to 10% formalin for processing with everything else, are the benefits of better nuclear morphology still likely to be realized? Thank you. Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 "Success is not the key to happiness. Happiness is the key to success. If you love what you are doing, you will be successful." - Albert Schweitzer ------------------------- Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ? l'intention d'une personne ou d'un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer imm?diatement de votre syst?me informatique. ------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Fri, 18 Jul 2008 11:50:18 -0500 From: "Herrick, James L." Subject: FW: [Histonet] Question on Aluminum Staining To: Message-ID: Content-Type: text/plain; charset="us-ascii" -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Herrick, James L. Sent: Thursday, July 17, 2008 3:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question on Aluminum Staining Hi everyone, We are having trouble running ASA (acid solochrome azurin) stains on human iliac crest bone biopsies for the detection of aluminum deposits. Our negatives are showing a lot of blue around the outer edges of the specimen. Although we cannot see staining around the trabecular bone, we should also not see it around the periphery of the bone specimen. The positive, on the other hand, looks very good. Has anyone had experience with this type of stain, and if so, would you happen to know what we may be doing wrong? The recipe we use for our stain is .2 gms. of Chromeazural B in 20mL of dH2O with a pH @ 5.0 (We incubate at room temp for 18hrs.), destain with 95% methanol, rinse in dH2O, dehydrate, clear and coverslip. Also, another problem we have encountered in the past, is a gold discoloring of the specimen. Have you seen this problem before, and if so, do you know how to correct it? Any help we can get with this dilemma is very much appreciated. Thanks again, Jim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 56, Issue 21 **************************************** From Jason.Burrill <@t> crl.com Sat Jul 19 12:38:10 2008 From: Jason.Burrill <@t> crl.com (Burrill, Jason) Date: Sat Jul 19 12:38:23 2008 Subject: [Histonet] RE: Formalin in plastic bags In-Reply-To: References: Message-ID: <1AD4E907E9B6F648AEF1B3A20A9B0E1E013B8E43@shr-exch2.na01.crl.com> Patricia you are correct about the classification of most formalin solutions being hazardous for air transport but as it relates to 10% neutral buffered formalin it does not apply. IATA regulations, which govern air transports, clearly state in the Dangerous Goods Regulations that any solution containing less than 10% formaldehyde is not considered hazardous for air transport. And since 10% neutral buffered formalin contains only 4% formaldehyde it is not considered a dangerous good for transport. I will say that you should always check with your commercial carrier (e.g. FedEx, UPS) before final classification of any material that there is a question about as they may have stricter requirements. Now if you are speaking about the transport of Patient Specimens as defined by IATA, exempt human or animal, which formalin fixed tissue typically falls into then what is required is a leak-proof primary receptacle, leak-proof secondary packaging and a rigid outer packaging with size dimensions of no less than 100 mm x 100 mm. And my final disclaimer will be that you should always check with your EHS department to make sure that you are following your institutional/company policies. Regards, Jason Jason Burrill Sr. Manager, Histology and Laboratory Safety Research Animal Diagnostic Services Charles River 251 Ballardvale St Wilmington, MA 01887 Office: 781-222-6152 Fax: 978-988-8793 jason.burrill@crl.com www.criver.com Accelerating Drug Development. Exactly. Notice - This email and any files transmitted with it are confidential and may contain privileged and/or proprietary information. You must not disclose this message to another party without Charles River's express written consent. If you are not the intended recipient you must not copy, distribute or use this email or the information contained in it for any purpose other than to notify us. If you have received this message in error, please notify Charles River immediately, and delete it from your system. ------------------------------ Message: 2 Date: Fri, 18 Jul 2008 13:34:46 -0400 From: "Patricia Karlisch" Subject: [Histonet] Formalin in plastic bags To: Message-ID: <48809BF6.07F7.008C.0@hmc.psu.edu> Content-Type: text/plain; charset=US-ASCII Richard, There are standards for sending specimens that are in a hazardous solution, like Formalin. Plastic bags are secondary containers and should not be used to transport fluid. The specimens should be in a secure plastic container that seals tightly and will not puncture causing injury to others. Pat Karlisch Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. From hilda_1075 <@t> yahoo.com Sat Jul 19 21:56:28 2008 From: hilda_1075 <@t> yahoo.com (shazana hilda) Date: Sat Jul 19 21:56:31 2008 Subject: [Histonet] troubleshoot of positive controls for PKCalpha and eNOS antibody Message-ID: <939284.12676.qm@web38806.mail.mud.yahoo.com> Dear Histonetters, ? I'm having difficulties to obtain positive staining?of positive control for PKCalpha (H-7:sc-8393) and NOS3(c-20:sc-654) antibody.?Both antibody were purchased from SantaCruz Biotechnology Inc.and used for?IHC.Below?are the methods that i used: ? 1)PKCalpha ??? -incubation time: 1hour,room temperature (24-25 celcius) ??? -Antibody dilution: 1:50 ??? - Target Antigen Retrieval Buffer: Citrate Buffer, pH6 ??? -Device: microwave (3x at 600W for 5min) ??? -Detection method: polymeric method by LabVision 2) NOS3@eNOS ????-incubation time: 1hour,room temperature (24-25 celcius) ??? -Antibody dilution: 1:50 ??? - Target Antigen Retrieval Buffer: Citrate Buffer, pH6 ??? -Device: microwave (3x at 600W for 5min) ??? -Detection method: polymeric method by LabVision ? I've used?normal rat kidney as suggested by few journals for?positive controls of both antibody. however my positive controls produced negative staining. ? Your?suggestion?and quick response are?highly appreciated ? Shazana Hilda Shamsuddin MSc.of Molecular Pathology, Dept. of Pathology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia. Email: hilda_1075@yahoo.com ? From Shirley_PHUA <@t> hsa.gov.sg Sun Jul 20 13:04:15 2008 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Sun Jul 20 13:04:32 2008 Subject: [Histonet] Shirley Phua is out-of-office ... Message-ID: I will be out of the office from 21-07-2008 to 22-07-2008. I'll be away on course 21-22 July 2008. Pathologists: I will process your requests when I return. If urgent, please forward your email to Henry_Kyaw@hsa.gov.sg From hlee <@t> medsci.usyd.edu.au Mon Jul 21 01:39:46 2008 From: hlee <@t> medsci.usyd.edu.au (Hyunchul Lee) Date: Mon Jul 21 01:39:50 2008 Subject: [Histonet] Rapid Golgi Message-ID: <204741.91719.qm@web31405.mail.mud.yahoo.com> Hello, I've been trying out this 'osmium-free' rapid Golgi protocol (not Golgi-Cox), and it seems to work- However, I've been having a couple of problems... After impregnation with silver nitrate (0.75%) solution, I immersed my brain in 30% sucrose for 24 hrs, then sectioned it on a freezing microtome. Out of my eagerness, I mounted a section and viewed it under a microscope- I was quite impressed! As it was getting late, I returned my sections to distilled water so that I could mount them the follwing day. Upon returning the next day, I mounted my sections. I hadn't coverslipped them yet... I viewed these again under the microscope and found that the staining I had seen earlier was nowhere to be seen! All I could see were brown blotches roughly corresponding to where the perikarya were. Google gave me the impression that others have been having similar problems regarding the longevity of Golgi-stained sections. Is there a way to stop this decomposition of rapid-Golgi product? We don't have Canada-balsam though... I had been hoping to perform immunohistochemistry on these same sections, and I have heard of people immunolabeling sections AFTER Golgi labeling, yet if the Golgi product has such a short life-span in water, this would be impossible. Is there a way to stop this decay in rapid Golgi material? Thank you in advance, Hyunchul Lee PhD student Systems Neuroscience Laboratory N121 Anderson Stuart Bldg. (F13) The University of Sydney, NSW, 2006 Email: hlee@medsci.usyd.edu.au From akbitting <@t> geisinger.edu Mon Jul 21 07:20:40 2008 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Mon Jul 21 07:20:52 2008 Subject: [Histonet] waste from Sakura Xpress Message-ID: <488446D8.2B7F.00C9.0@geisinger.edu> How are folks disposing of their waste from the Sakura Xpress processors? I welcome the vendors input on this too. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From Pkarlisch <@t> hmc.psu.edu Mon Jul 21 08:28:11 2008 From: Pkarlisch <@t> hmc.psu.edu (Patricia Karlisch) Date: Mon Jul 21 08:28:35 2008 Subject: [Histonet] Re: Histonet Digest, Vol 56, Issue 23 In-Reply-To: <9d733884.287@gwia03.hersheymed.net> References: <9d733884.287@gwia03.hersheymed.net> Message-ID: <488456AB.07F7.008C.0@hmc.psu.edu> Jason, I agree with you about Air Transportation and their specific rules governing Air Transport, but hospitals have different requirements in transporting hazardous materials within a hospital, from lab to lab or clinic site to lab or by courier to lab. The secondary container is for the protection of the transporter (hospital) and the specimen integrity. I may be reading the email wrong from Richard, but it sounded like specimens were being transported in the equivalent of a zip lock bag. If I was wrong I apologise. Pat Karlisch Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. >>> 7/20/2008 1:04 PM >>> Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Formalin in plastic bags (Burrill, Jason) 2. troubleshoot of positive controls for PKCalpha and eNOS antibody (shazana hilda) ---------------------------------------------------------------------- Message: 1 Date: Sat, 19 Jul 2008 13:38:10 -0400 From: "Burrill, Jason" Subject: [Histonet] RE: Formalin in plastic bags To: , Message-ID: <1AD4E907E9B6F648AEF1B3A20A9B0E1E013B8E43@shr-exch2.na01.crl.com> Content-Type: text/plain;charset="US-ASCII" Patricia you are correct about the classification of most formalin solutions being hazardous for air transport but as it relates to 10% neutral buffered formalin it does not apply. IATA regulations, which govern air transports, clearly state in the Dangerous Goods Regulations that any solution containing less than 10% formaldehyde is not considered hazardous for air transport. And since 10% neutral buffered formalin contains only 4% formaldehyde it is not considered a dangerous good for transport. I will say that you should always check with your commercial carrier (e.g. FedEx, UPS) before final classification of any material that there is a question about as they may have stricter requirements. Now if you are speaking about the transport of Patient Specimens as defined by IATA, exempt human or animal, which formalin fixed tissue typically falls into then what is required is a leak-proof primary receptacle, leak-proof secondary packaging and a rigid outer packaging with size dimensions of no less than 100 mm x 100 mm. And my final disclaimer will be that you should always check with your EHS department to make sure that you are following your institutional/company policies. Regards, Jason Jason Burrill Sr. Manager, Histology and Laboratory Safety Research Animal Diagnostic Services Charles River 251 Ballardvale St Wilmington, MA 01887 Office: 781-222-6152 Fax: 978-988-8793 jason.burrill@crl.com www.criver.com Accelerating Drug Development. Exactly. Notice - This email and any files transmitted with it are confidential and may contain privileged and/or proprietary information. You must not disclose this message to another party without Charles River's express written consent. If you are not the intended recipient you must not copy, distribute or use this email or the information contained in it for any purpose other than to notify us. If you have received this message in error, please notify Charles River immediately, and delete it from your system. ------------------------------ Message: 2 Date: Fri, 18 Jul 2008 13:34:46 -0400 From: "Patricia Karlisch" Subject: [Histonet] Formalin in plastic bags To: Message-ID: <48809BF6.07F7.008C.0@hmc.psu.edu> Content-Type: text/plain; charset=US-ASCII Richard, There are standards for sending specimens that are in a hazardous solution, like Formalin. Plastic bags are secondary containers and should not be used to transport fluid. The specimens should be in a secure plastic container that seals tightly and will not puncture causing injury to others. Pat Karlisch Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. ------------------------------ Message: 2 Date: Sat, 19 Jul 2008 19:56:28 -0700 (PDT) From: shazana hilda Subject: [Histonet] troubleshoot of positive controls for PKCalpha and eNOSantibody To: histonet@lists.utsouthwestern.edu Message-ID: <939284.12676.qm@web38806.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Dear Histonetters, I'm having difficulties to obtain positive staining of positive control for PKCalpha (H-7:sc-8393) and NOS3(c-20:sc-654) antibody. Both antibody were purchased from SantaCruz Biotechnology Inc.and used for IHC.Below are the methods that i used: 1)PKCalpha -incubation time: 1hour,room temperature (24-25 celcius) -Antibody dilution: 1:50 - Target Antigen Retrieval Buffer: Citrate Buffer, pH6 -Device: microwave (3x at 600W for 5min) -Detection method: polymeric method by LabVision 2) NOS3@eNOS -incubation time: 1hour,room temperature (24-25 celcius) -Antibody dilution: 1:50 - Target Antigen Retrieval Buffer: Citrate Buffer, pH6 -Device: microwave (3x at 600W for 5min) -Detection method: polymeric method by LabVision I've used normal rat kidney as suggested by few journals for positive controls of both antibody. however my positive controls produced negative staining. Your suggestion and quick response are highly appreciated Shazana Hilda Shamsuddin MSc.of Molecular Pathology, Dept. of Pathology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia. Email: hilda_1075@yahoo.com ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 56, Issue 23 **************************************** From beingmary53 <@t> sbcglobal.net Mon Jul 21 09:59:35 2008 From: beingmary53 <@t> sbcglobal.net (MARY JOHNSON) Date: Mon Jul 21 09:59:39 2008 Subject: [Histonet] (no subject) Message-ID: <207645.64703.qm@web81605.mail.mud.yahoo.com> Is there something wrong with the histonet? I have not received anything from you far a while my e-mail is beingmary53@sbcglobal.net Please check my e-mail address. Thank you Mary From KLAPANO1 <@t> hfhs.org Mon Jul 21 10:38:10 2008 From: KLAPANO1 <@t> hfhs.org (Karen Lapanowski) Date: Mon Jul 21 10:38:30 2008 Subject: [Histonet] Albumin Antibody Message-ID: <488475220200002800009336@gwia2v.net.hfh.edu> Hello, Can anyone recommend a supplier and/or protocol for staining albumin in fixed 100 micron vibratome section of rat brain? Thanks, Karen Lapanowski Henry Ford Health System Radiation Oncology 3065 E & R 313-916-9386 ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== From mike <@t> pathview.com Mon Jul 21 10:45:43 2008 From: mike <@t> pathview.com (Michael Mihalik) Date: Mon Jul 21 10:45:51 2008 Subject: [Histonet] AP Cerner Copath vs Sunquest Copath vs others In-Reply-To: References: <487F5E8302000077000042AD@gwmail6.harthosp.org> <37E7D6EF2B8D440DB16B613E1E6D7A87@dielangs.at> Message-ID: <031001c8eb48$d5c57780$81506680$@com> Good day, I wanted to wait a few days before I responded to this request as I represent a system vendor. I would love to talk to you about our system and why your organization should consider us, but I suspect this would be best dealt with via direct email. To the group, I pose this question: I try to be very conscientious of the fact that this method of communication and interaction is intended to be primarily between histology personnel and that vendors should be very respectful of that idiom. Do you think my offer above is appropriate? Would you like to see our dialog? I'd appreciate any feedback that you can provide. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristian Britos Sent: Thursday, July 17, 2008 3:26 PM To: Richard Cartun; gu.lang@gmx.at; histonet@lists.utsouthwestern.edu Subject: [Histonet] AP Cerner Copath vs Sunquest Copath vs others Would someone be willing to share their experiences about Sunquest copath as their LIS system for pathology versus other systems? Needless to say, we are looking for options.... (currently we have cerner copath 3.0) Thanks, Cris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From melissa.mazan <@t> tufts.edu Mon Jul 21 13:10:35 2008 From: melissa.mazan <@t> tufts.edu (Melissa Mazan) Date: Mon Jul 21 13:10:39 2008 Subject: [Histonet] cryostat In-Reply-To: <200807211703.m6LH3AlZ001794@mail-proofpoint-2a.usg.tufts.edu> References: <200807211703.m6LH3AlZ001794@mail-proofpoint-2a.usg.tufts.edu> Message-ID: <20080721141035.bytwob5oasgkoow0@webmail.tufts.edu> Hi all, I am looking to buy a cryostat - I have now got a Bright Starlet 2212 with which I have had a lot of problems. Does anyone have suggestions for a cryostat? We are a small research lab with limited funds. Thanks - Melissa Quoting histonet-request@lists.utsouthwestern.edu: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Shirley Phua is out-of-office ... (Shirley PHUA) > 2. Rapid Golgi (Hyunchul Lee) > 3. waste from Sakura Xpress (Angela Bitting) > 4. Re: Histonet Digest, Vol 56, Issue 23 (Patricia Karlisch) > 5. (no subject) (MARY JOHNSON) > 6. Albumin Antibody (Karen Lapanowski) > 7. RE: AP Cerner Copath vs Sunquest Copath vs others > (Michael Mihalik) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 21 Jul 2008 02:04:15 +0800 > From: Shirley PHUA > Subject: [Histonet] Shirley Phua is out-of-office ... > To: histonet > Message-ID: > > > Content-Type: text/plain; charset=US-ASCII > > I will be out of the office from 21-07-2008 to 22-07-2008. > > I'll be away on course 21-22 July 2008. > > Pathologists: > I will process your requests when I return. If urgent, please forward your > email to Henry_Kyaw@hsa.gov.sg > > ------------------------------ > > Message: 2 > Date: Sun, 20 Jul 2008 23:39:46 -0700 (PDT) > From: Hyunchul Lee > Subject: [Histonet] Rapid Golgi > To: histonet@lists.utsouthwestern.edu > Message-ID: <204741.91719.qm@web31405.mail.mud.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > Hello, > > I've been trying out this 'osmium-free' rapid Golgi protocol (not > Golgi-Cox), and it seems to work- > However, I've been having a couple of problems... > > After impregnation with silver nitrate (0.75%) solution, I immersed > my brain in 30% sucrose for 24 hrs, > then sectioned it on a freezing microtome. Out of my eagerness, I > mounted a section and viewed it > under a microscope- I was quite impressed! As it was getting late, I > returned my sections to distilled water > so that I could mount them the follwing day. > > Upon returning the next day, I mounted my sections. I hadn't > coverslipped them yet... > I viewed these again under the microscope and found that the staining > I had seen earlier was nowhere to be seen! > All I could see were brown blotches roughly corresponding to where > the perikarya were. > Google gave me the impression that others have been having similar > problems regarding the longevity of > Golgi-stained sections. Is there a way to stop this decomposition of > rapid-Golgi product? > We don't have Canada-balsam though... > > I had been hoping to perform immunohistochemistry on these same > sections, and I have heard of people > immunolabeling sections AFTER Golgi labeling, yet if the Golgi > product has such a short life-span in water, > this would be impossible. Is there a way to stop this decay in rapid > Golgi material? > > Thank you in advance, > > > Hyunchul Lee > PhD student > > Systems Neuroscience Laboratory > N121 Anderson Stuart Bldg. (F13) > The University of Sydney, NSW, 2006 > Email: hlee@medsci.usyd.edu.au > > > > > ------------------------------ > > Message: 3 > Date: Mon, 21 Jul 2008 08:20:40 -0400 > From: "Angela Bitting" > Subject: [Histonet] waste from Sakura Xpress > To: > Message-ID: <488446D8.2B7F.00C9.0@geisinger.edu> > Content-Type: text/plain; charset="us-ascii" > > How are folks disposing of their waste from the Sakura Xpress > processors? I welcome the vendors input on this too. > > Angela Bitting, HT(ASCP) > Technical Specialist, Histology > Geisinger Medical Center > 100 N Academy Ave. MC 23-00 > Danville, PA 17822 > phone 570-214-9634 > fax 570-271-5916 > > No trees were hurt in the sending of this email > However many electrons were severly inconvienienced! > > > > > IMPORTANT WARNING: The information in this message (and the documents > attached to it, if any) is confidential and may be legally > privileged. It is intended solely for the addressee. Access to this > message by anyone else is unauthorized. If you are not the intended > recipient, any disclosure, copying, distribution or any action taken, > or omitted to be taken, in reliance on it is prohibited and may be > unlawful. If you have received this message in error, please delete > all electronic copies of this message (and the documents attached to > it, if any), destroy any hard copies you may have created and notify > me immediately by replying to this email. Thank you. > -------------- next part -------------- > BEGIN:VCARD > VERSION:2.1 > X-GWTYPE:USER > FN:Bitting, Angela > TEL;WORK:570-271-6844 > ORG:;Histology > EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu > N:Bitting;Angela > END:VCARD > > > ------------------------------ > > Message: 4 > Date: Mon, 21 Jul 2008 09:28:11 -0400 > From: "Patricia Karlisch" > Subject: [Histonet] Re: Histonet Digest, Vol 56, Issue 23 > To: > Message-ID: <488456AB.07F7.008C.0@hmc.psu.edu> > Content-Type: text/plain; charset=US-ASCII > > Jason, > I agree with you about Air Transportation and their specific rules > governing Air Transport, but hospitals have different requirements in > transporting hazardous materials within a hospital, from lab to lab > or clinic site to lab or by courier to lab. The secondary container > is for the protection of the transporter (hospital) and the specimen > integrity. > I may be reading the email wrong from Richard, but it sounded like > specimens were being transported in the equivalent of a zip lock bag. > If I was wrong I apologise. > Pat Karlisch > > Pat Karlisch > Supervisor, Histology, Pathology and Laboratory Medicine > Penn State Milton S. Hershey Medical Center > Mail Code H179 > Hershey, PA 17033 > Phone (717) 531-6072 > Fax: (717) 531- 7741 > email: pkarlisch@psu.edu > > *****E-Mail Confidentiality Notice***** > This message (including any attachments) contains information > intended for a specific individual(s) and purpose that may be > privileged, confidential or otherwise protected from disclosure > pursuant to applicable law. Any inappropriate use, distribution or > copying of the message is strictly prohibited and may subject you to > criminal or civil penalty. If you have received this transmission in > error, please reply to the sender indicating this error and delete > the transmission from your system immediately. > > >>>> 7/20/2008 1:04 PM >>> > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Formalin in plastic bags (Burrill, Jason) > 2. troubleshoot of positive controls for PKCalpha and eNOS > antibody (shazana hilda) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sat, 19 Jul 2008 13:38:10 -0400 > From: "Burrill, Jason" > Subject: [Histonet] RE: Formalin in plastic bags > To: , > Message-ID: > <1AD4E907E9B6F648AEF1B3A20A9B0E1E013B8E43@shr-exch2.na01.crl.com> > Content-Type: text/plain;charset="US-ASCII" > > Patricia you are correct about the classification of most formalin > solutions being hazardous for air transport but as it relates to 10% > neutral buffered formalin it does not apply. IATA regulations, which > govern air transports, clearly state in the Dangerous Goods Regulations > that any solution containing less than 10% formaldehyde is not > considered hazardous for air transport. And since 10% neutral buffered > formalin contains only 4% formaldehyde it is not considered a dangerous > good for transport. I will say that you should always check with your > commercial carrier (e.g. FedEx, UPS) before final classification of any > material that there is a question about as they may have stricter > requirements. > > > > Now if you are speaking about the transport of Patient Specimens as > defined by IATA, exempt human or animal, which formalin fixed tissue > typically falls into then what is required is a leak-proof primary > receptacle, leak-proof secondary packaging and a rigid outer packaging > with size dimensions of no less than 100 mm x 100 mm. > > > > And my final disclaimer will be that you should always check with your > EHS department to make sure that you are following your > institutional/company policies. > > > > Regards, > > Jason > > > > Jason Burrill > > Sr. Manager, Histology and Laboratory Safety > > Research Animal Diagnostic Services > > Charles River > > 251 Ballardvale St > > Wilmington, MA 01887 > > Office: 781-222-6152 > > Fax: 978-988-8793 > > jason.burrill@crl.com > > www.criver.com > > > > Accelerating Drug Development. Exactly. > > > > Notice - This email and any files transmitted with it are confidential > and may contain privileged and/or proprietary information. You must not > disclose this message to another party without Charles River's express > written consent. If you are not the intended recipient you must not > copy, distribute or use this email or the information contained in it > for any purpose other than to notify us. If you have received this > message in error, please notify Charles River immediately, and delete it > from your system. > > > > ------------------------------ > > > > Message: 2 > > Date: Fri, 18 Jul 2008 13:34:46 -0400 > > From: "Patricia Karlisch" > > Subject: [Histonet] Formalin in plastic bags > > To: > > Message-ID: <48809BF6.07F7.008C.0@hmc.psu.edu> > > Content-Type: text/plain; charset=US-ASCII > > > > Richard, > > There are standards for sending specimens that are in a hazardous > solution, like Formalin. Plastic bags are secondary containers and > should not be used to transport fluid. The specimens should be in a > secure plastic container that seals tightly and will not puncture > causing injury to others. > > Pat Karlisch > > > > Pat Karlisch > > Supervisor, Histology, Pathology and Laboratory Medicine > > Penn State Milton S. Hershey Medical Center > > Mail Code H179 > > Hershey, PA 17033 > > Phone (717) 531-6072 > > Fax: (717) 531- 7741 > > email: pkarlisch@psu.edu > > > > *****E-Mail Confidentiality Notice***** > > This message (including any attachments) contains information intended > for a specific individual(s) and purpose that may be privileged, > confidential or otherwise protected from disclosure pursuant to > applicable law. Any inappropriate use, distribution or copying of the > message is strictly prohibited and may subject you to criminal or civil > penalty. If you have received this transmission in error, please reply > to the sender indicating this error and delete the transmission from > your system immediately. > > > > > > > > ------------------------------ > > Message: 2 > Date: Sat, 19 Jul 2008 19:56:28 -0700 (PDT) > From: shazana hilda > Subject: [Histonet] troubleshoot of positive controls for PKCalpha and > eNOSantibody > To: histonet@lists.utsouthwestern.edu > Message-ID: <939284.12676.qm@web38806.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Dear Histonetters, > > I'm having difficulties to obtain positive staining of positive > control for PKCalpha (H-7:sc-8393) and NOS3(c-20:sc-654) antibody. > Both antibody were purchased from SantaCruz Biotechnology Inc.and > used for IHC.Below are the methods that i used: > > 1)PKCalpha > -incubation time: 1hour,room temperature (24-25 celcius) > -Antibody dilution: 1:50 > - Target Antigen Retrieval Buffer: Citrate Buffer, pH6 > -Device: microwave (3x at 600W for 5min) > -Detection method: polymeric method by LabVision > 2) NOS3@eNOS > -incubation time: 1hour,room temperature (24-25 celcius) > -Antibody dilution: 1:50 > - Target Antigen Retrieval Buffer: Citrate Buffer, pH6 > -Device: microwave (3x at 600W for 5min) > -Detection method: polymeric method by LabVision > > I've used normal rat kidney as suggested by few journals for positive > controls of both antibody. however my positive controls produced > negative staining. > > Your suggestion and quick response are highly appreciated > > Shazana Hilda Shamsuddin > MSc.of Molecular Pathology, > Dept. of Pathology, > School of Medical Sciences, > Universiti Sains Malaysia, > 16150 Kubang Kerian, > Kelantan, Malaysia. > Email: hilda_1075@yahoo.com > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 56, Issue 23 > **************************************** > > > ------------------------------ > > Message: 5 > Date: Mon, 21 Jul 2008 07:59:35 -0700 (PDT) > From: MARY JOHNSON > Subject: [Histonet] (no subject) > To: Histonet@lists.utsouthwestern.edu > Message-ID: <207645.64703.qm@web81605.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Is there something wrong with the histonet? I have not received > anything from you far a while > my e-mail is beingmary53@sbcglobal.net Please check my e-mail address. > Thank you Mary > > > ------------------------------ > > Message: 6 > Date: Mon, 21 Jul 2008 11:38:10 -0400 > From: "Karen Lapanowski" > Subject: [Histonet] Albumin Antibody > To: histonet@lists.utsouthwestern.edu > Message-ID: <488475220200002800009336@gwia2v.net.hfh.edu> > Content-Type: text/plain; charset=us-ascii > > Hello, > Can anyone recommend a supplier and/or protocol for staining albumin > in fixed 100 micron vibratome section of rat brain? > Thanks, > > Karen Lapanowski > Henry Ford Health System > Radiation Oncology > 3065 E & R > 313-916-9386 > > > ============================================================================== > CONFIDENTIALITY NOTICE: This email contains information from the > sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or > otherwise protected from disclosure. This email is intended for use > only by the person or entity to whom it is addressed. If you are not > the intended recipient, any use, disclosure, copying, distribution, > printing, or any action taken in reliance on the contents of this > email, is strictly prohibited. If you received this email in error, > please contact the sending party by reply email, delete the email > from your computer system and shred any paper copies. > > Note to Patients: There are a number of risks you should consider > before using e-mail to communicate with us. See our Privacy Policy > and Henry Ford My Health at www.henryford.com for more detailed > information. If you do not believe that our policy gives you the > privacy and security protection you need, do not send e-mail or > Internet communications to us. > > ============================================================================== > > > > > ------------------------------ > > Message: 7 > Date: Mon, 21 Jul 2008 11:45:43 -0400 > From: "Michael Mihalik" > Subject: RE: [Histonet] AP Cerner Copath vs Sunquest Copath vs others > To: "'Cristian Britos'" , "'Richard Cartun'" > , , > > Message-ID: <031001c8eb48$d5c57780$81506680$@com> > Content-Type: text/plain; charset="US-ASCII" > > Good day, > > I wanted to wait a few days before I responded to this request as I > represent a system vendor. I would love to talk to you about our system > and why your organization should consider us, but I suspect this would be > best dealt with via direct email. > > To the group, I pose this question: > > I try to be very conscientious of the fact that this method of communication > and interaction is intended to be primarily between histology personnel and > that vendors should be very respectful of that idiom. Do you think my offer > above is appropriate? Would you like to see our dialog? > > I'd appreciate any feedback that you can provide. > > Michael Mihalik > PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristian > Britos > Sent: Thursday, July 17, 2008 3:26 PM > To: Richard Cartun; gu.lang@gmx.at; histonet@lists.utsouthwestern.edu > Subject: [Histonet] AP Cerner Copath vs Sunquest Copath vs others > > Would someone be willing to share their experiences about Sunquest > copath as their LIS system for pathology versus other systems? > Needless to say, we are looking for options.... (currently we have > cerner copath 3.0) > Thanks, > Cris > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 56, Issue 24 > **************************************** > Melissa R. Mazan, DVM, Diplomate ACVIM Associate Professor and Director of Equine Sports Medicine Department of Clinical Sciences Tufts Cumming School of Veterinary Medicine 200 Westborough Road North Grafton, MA 01536 Tel:508-839-5395 Fax:508-839-7922 email: melissa.mazan@tufts.edu From PMonfils <@t> Lifespan.org Mon Jul 21 13:15:19 2008 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Mon Jul 21 13:15:24 2008 Subject: [Histonet] "+" slides for sections of plant tissue Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835C30@LSRIEXCH1.lsmaster.lifespan.org> Does anyone have experience using "+" charged slides with plant sections? How well do they work? From wdesalvo.cac <@t> hotmail.com Mon Jul 21 13:28:14 2008 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Mon Jul 21 13:28:19 2008 Subject: [Histonet] waste from Sakura Xpress In-Reply-To: <488446D8.2B7F.00C9.0@geisinger.edu> References: <488446D8.2B7F.00C9.0@geisinger.edu> Message-ID: We have two Xpress 120's and have been using them for 3+ years. At our facility and in the city of Tempe, AZ, we place the liquid waste, two gallons, in our 55 gallon waste disposal drums w/ other flammable and aqueous solutions (Clean Harbors Disposal). The paraffin is drained, allowed to harden and placed in non-hazardous solid waste. William DeSalvo BS, HTL(ASCP)Production ManagerSonora Quest Labs > Date: Mon, 21 Jul 2008 08:20:40 -0400> From: akbitting@geisinger.edu> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] waste from Sakura Xpress> > How are folks disposing of their waste from the Sakura Xpress processors? I welcome the vendors input on this too.> > Angela Bitting, HT(ASCP)> Technical Specialist, Histology> Geisinger Medical Center > 100 N Academy Ave. MC 23-00> Danville, PA 17822> phone 570-214-9634> fax 570-271-5916 > > No trees were hurt in the sending of this email> However many electrons were severly inconvienienced!> > > > > IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _________________________________________________________________ Time for vacation? WIN what you need- enter now! http://www.gowindowslive.com/summergiveaway/?ocid=tag_jlyhm From Herrick.James <@t> mayo.edu Tue Jul 22 10:10:28 2008 From: Herrick.James <@t> mayo.edu (Herrick, James L.) Date: Tue Jul 22 10:10:39 2008 Subject: FW: [Histonet] Question on Aluminum Staining Message-ID: -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Herrick, James L. Sent: Friday, July 18, 2008 11:50 AM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] Question on Aluminum Staining -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Herrick, James L. Sent: Thursday, July 17, 2008 3:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question on Aluminum Staining Hi everyone, We are having trouble running ASA (acid solochrome azurin) stains on human iliac crest bone biopsies for the detection of aluminum deposits. Our negatives are showing a lot of blue around the outer edges of the specimen. Although we cannot see staining around the trabecular bone, we should also not see it around the periphery of the bone specimen. The positive, on the other hand, looks very good. Has anyone had experience with this type of stain, and if so, would you happen to know what we may be doing wrong? The recipe we use for our stain is .2 gms. of Chromeazural B in 20mL of dH2O with a pH @ 5.0 (We incubate at room temp for 18hrs.), destain with 95% methanol, rinse in dH2O, dehydrate, clear and coverslip. Also, another problem we have encountered in the past, is a gold discoloring of the specimen. Have you seen this problem before, and if so, do you know how to correct it? Any help we can get with this dilemma is very much appreciated. Thanks again, Jim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Tue Jul 22 10:23:20 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Tue Jul 22 10:26:28 2008 Subject: [Histonet] NSH Teleconferences Message-ID: <08A0A863637F1349BBFD83A96B27A50A120153@uwhis-xchng3.uwhis.hosp.wisc.edu> Hey there gang: Does anyone know who to contact to get the site to download the info for tomorrow's NSH teleconference? I have again failed to recieve this info. If anyone from NSH can contact me, it would be much appreciated. Claire Ingles UW Hospital and Clinics Madison WI From vvancleave <@t> hendrickhealth.org Tue Jul 22 10:43:17 2008 From: vvancleave <@t> hendrickhealth.org (Vancleave, Vince) Date: Tue Jul 22 10:41:41 2008 Subject: [Histonet] video for infection control training and/or OSHA safety? Message-ID: <740F77F6594C20458BDC2A4A500D8D4109B49BF7@EMAIL.hhs.hendrickhealth.org> Well, we are looking to update our safety training methods and find some video compilations that cover OSHA laboratory safety and infection control. Do any of you out there really have one you like that you would recommend? Thanks a bunches everyone! Hope everybody is doing great! Vince Van Cleave, PA(ASCP)CM, HTL(ASCP), HT(ASCP) Pathologists' Assistant and Laboratory Manager Clinical Pathology Associates, Abilene, TX 1150 Nth 18th STE 102, 79601 325-670-6528 PRIVILEGED AND CONFIDENTIAL NOTICE: The information contained in this e-mail may be confidential and/or privileged. This e-mail is intended to be reviewed initially by only the individual named above. If the reader of this e-mail is not the intended recipient or a representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail, or the information contained herein, is prohibited. If you have received this e-mail in error, notify the above sender, send the message back and then delete it. Thank you for your assistance. Hendrick Health System 1900 Pine St. Abilene, Texas 79601 - 325.670.2000 79601 - 325.670.2000 From peter.craven <@t> nhs.net Tue Jul 22 11:03:15 2008 From: peter.craven <@t> nhs.net (peter.craven@nhs.net) Date: Tue Jul 22 11:03:21 2008 Subject: [Histonet] (no subject) Message-ID: <000a01c8ec14$73949ec0$2603030a@RAIGMOREAD.HAHT.SCOT.NHS.UK> Hi folks We are in the process of reviewing our staining solutions and times for our routine Haematoxylin and Eosin; at present we use in house solutions and are looking to reduce the staining times in use at present on our Leica Autostainer XL. Would anyone like to comment on possible sources and timings for stains (UK based suppliers please) Peter Craven FIBMS Raigmore Hospital Inverness IV2 3UJ *********************************************************************** This message may contain confidential and privileged information. If you are not the intended recipient you should not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents. To do so is strictly prohibited and may be unlawful. Please inform the sender that this message has gone astray before deleting it. Thank you. 2008 marks the 60th anniversary of the NHS. It's an opportunity to pay tribute to the NHS staff and volunteers who help shape the service, and celebrate their achievements. If you work for the NHS and would like an NHSmail email account, go to: www.connectingforhealth.nhs.uk/nhsmail *********************************************************************** From hardin <@t> oncology.wisc.edu Tue Jul 22 11:11:33 2008 From: hardin <@t> oncology.wisc.edu (Joe Hardin) Date: Tue Jul 22 11:11:36 2008 Subject: [Histonet] paraffin embedded formalin fixed human tissue Message-ID: <488606B5.50902@oncology.wisc.edu> Hi Everyone, I work in a core histology facility for researchers here at University of Wisconsin, Madison. I am looking for affordable paraffin embedded formalin fixed human tissue to use as control tissue for immunohistochemistry. The cheapest that I can find is >500.00/block. Does anyone know of a good source for this? From ree3 <@t> leicester.ac.uk Tue Jul 22 11:18:19 2008 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Tue Jul 22 11:18:35 2008 Subject: [Histonet] FW: NSH Teleconferences Message-ID: <7722595275A4DD4FA225B92CDBF174A17438DEDB9C@EXC-MBX3.cfs.le.ac.uk> -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: 22 July 2008 16:23 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NSH Teleconferences Hey there gang: Does anyone know who to contact to get the site to download the info for tomorrow's NSH teleconference? I have again failed to recieve this info. If anyone from NSH can contact me, it would be much appreciated. Claire Ingles UW Hospital and Clinics Madison WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Tue Jul 22 11:19:08 2008 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Tue Jul 22 11:19:14 2008 Subject: [Histonet] FW: NOS Message-ID: <7722595275A4DD4FA225B92CDBF174A17438DEDB9D@EXC-MBX3.cfs.le.ac.uk> ----S Anyone experience of immunocytochemistry with the eNOS, iNOS and bNOS antibodies available from Abcam or anywhere else; to be used on cryostat sections of mouse heart. Many thanks Richard Edwards Leicester University...U.K.. From anh2006 <@t> med.cornell.edu Tue Jul 22 11:44:25 2008 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Tue Jul 22 11:47:14 2008 Subject: [Histonet] paraffin embedded formalin fixed human tissue Message-ID: <274468461-1216745224-cardhu_decombobulator_blackberry.rim.net-1184922124-@bxe157.bisx.prod.on.blackberry> Cooperative Human Tissue Network (CHTN) ------Original Message------ From: Joe Hardin Sender: histonet-bounces@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Jul 22, 2008 12:11 PM Subject: [Histonet] paraffin embedded formalin fixed human tissue Hi Everyone, I work in a core histology facility for researchers here at University of Wisconsin, Madison. I am looking for affordable paraffin embedded formalin fixed human tissue to use as control tissue for immunohistochemistry. The cheapest that I can find is >500.00/block. Does anyone know of a good source for this? From TJJ <@t> Stowers-Institute.org Tue Jul 22 12:10:14 2008 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Tue Jul 22 12:10:31 2008 Subject: [Histonet] NSH teleconference email contact Message-ID: Contact brenda@nsh.org Claire wrote: Hey there gang: Does anyone know who to contact to get the site to download the info for tomorrow's NSH teleconference? I have again failed to recieve this info. If anyone from NSH can contact me, it would be much appreciated. Claire Ingles UW Hospital and Clinics Madison WI Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From nancy.troiano <@t> yale.edu Tue Jul 22 12:17:32 2008 From: nancy.troiano <@t> yale.edu (Nancy W. Troiano) Date: Tue Jul 22 12:17:43 2008 Subject: [Histonet] Aluminum staining of bone Message-ID: <5.2.1.1.2.20080722131619.021c6520@email.med.yale.edu> We have a aluminon staining protocol for undecalcified, MMA embedded bone that I can share that may work for you. You can send me an email and a fax number and I can fax the protocol to you. From kmilne <@t> bccancer.bc.ca Tue Jul 22 15:00:35 2008 From: kmilne <@t> bccancer.bc.ca (Milne, Katy) Date: Tue Jul 22 15:00:45 2008 Subject: [Histonet] Replacement reagent bottles for Thermo Shandon PathCentre In-Reply-To: References: Message-ID: <07979E76B0869D4E8C9FE4AA9FC06578046692F6@srvex03.phsabc.ehcnet.ca> Hi all, I need to replace some of my reagent reservoirs for the Pathcentre (the white plastic ones that hold xylene and ethanol) and I'm being quoted over $1000 for 3 bottles. This seems a bit excessive to me so I'm wondering if anyone out there has a cheaper supplier I may be able to contact? The xylene bottles are swollen and are causing airflow problems with the machine, I've swapped in the spare three that came with the machine but I still have one to replace plus I'd like some back-up bottles. Any suggestions on cheaper suppliers if any are out there would be great! Thanks, Katy Milne Deeley Research Centre From mohs76009 <@t> yahoo.com Tue Jul 22 16:36:36 2008 From: mohs76009 <@t> yahoo.com (Matt Bancroft) Date: Tue Jul 22 16:36:44 2008 Subject: [Histonet] cryostat In-Reply-To: <20080721141035.bytwob5oasgkoow0@webmail.tufts.edu> Message-ID: <94057.73217.qm@web63411.mail.re1.yahoo.com> Microm 525 --- On Mon, 7/21/08, Melissa Mazan wrote: From: Melissa Mazan Subject: [Histonet] cryostat To: histonet@lists.utsouthwestern.edu Date: Monday, July 21, 2008, 1:10 PM Hi all, I am looking to buy a cryostat - I have now got a Bright Starlet 2212 with which I have had a lot of problems. Does anyone have suggestions for a cryostat? We are a small research lab with limited funds. Thanks - Melissa Quoting histonet-request@lists.utsouthwestern.edu: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Shirley Phua is out-of-office ... (Shirley PHUA) > 2. Rapid Golgi (Hyunchul Lee) > 3. waste from Sakura Xpress (Angela Bitting) > 4. Re: Histonet Digest, Vol 56, Issue 23 (Patricia Karlisch) > 5. (no subject) (MARY JOHNSON) > 6. Albumin Antibody (Karen Lapanowski) > 7. RE: AP Cerner Copath vs Sunquest Copath vs others > (Michael Mihalik) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 21 Jul 2008 02:04:15 +0800 > From: Shirley PHUA > Subject: [Histonet] Shirley Phua is out-of-office ... > To: histonet > Message-ID: > > > Content-Type: text/plain; charset=US-ASCII > > I will be out of the office from 21-07-2008 to 22-07-2008. > > I'll be away on course 21-22 July 2008. > > Pathologists: > I will process your requests when I return. If urgent, please forward your > email to Henry_Kyaw@hsa.gov.sg > > ------------------------------ > > Message: 2 > Date: Sun, 20 Jul 2008 23:39:46 -0700 (PDT) > From: Hyunchul Lee > Subject: [Histonet] Rapid Golgi > To: histonet@lists.utsouthwestern.edu > Message-ID: <204741.91719.qm@web31405.mail.mud.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > Hello, > > I've been trying out this 'osmium-free' rapid Golgi protocol (not > Golgi-Cox), and it seems to work- > However, I've been having a couple of problems... > > After impregnation with silver nitrate (0.75%) solution, I immersed > my brain in 30% sucrose for 24 hrs, > then sectioned it on a freezing microtome. Out of my eagerness, I > mounted a section and viewed it > under a microscope- I was quite impressed! As it was getting late, I > returned my sections to distilled water > so that I could mount them the follwing day. > > Upon returning the next day, I mounted my sections. I hadn't > coverslipped them yet... > I viewed these again under the microscope and found that the staining > I had seen earlier was nowhere to be seen! > All I could see were brown blotches roughly corresponding to where > the perikarya were. > Google gave me the impression that others have been having similar > problems regarding the longevity of > Golgi-stained sections. Is there a way to stop this decomposition of > rapid-Golgi product? > We don't have Canada-balsam though... > > I had been hoping to perform immunohistochemistry on these same > sections, and I have heard of people > immunolabeling sections AFTER Golgi labeling, yet if the Golgi > product has such a short life-span in water, > this would be impossible. Is there a way to stop this decay in rapid > Golgi material? > > Thank you in advance, > > > Hyunchul Lee > PhD student > > Systems Neuroscience Laboratory > N121 Anderson Stuart Bldg. (F13) > The University of Sydney, NSW, 2006 > Email: hlee@medsci.usyd.edu.au > > > > > ------------------------------ > > Message: 3 > Date: Mon, 21 Jul 2008 08:20:40 -0400 > From: "Angela Bitting" > Subject: [Histonet] waste from Sakura Xpress > To: > Message-ID: <488446D8.2B7F.00C9.0@geisinger.edu> > Content-Type: text/plain; charset="us-ascii" > > How are folks disposing of their waste from the Sakura Xpress > processors? I welcome the vendors input on this too. > > Angela Bitting, HT(ASCP) > Technical Specialist, Histology > Geisinger Medical Center > 100 N Academy Ave. MC 23-00 > Danville, PA 17822 > phone 570-214-9634 > fax 570-271-5916 > > No trees were hurt in the sending of this email > However many electrons were severly inconvienienced! > > > > > IMPORTANT WARNING: The information in this message (and the documents > attached to it, if any) is confidential and may be legally > privileged. It is intended solely for the addressee. Access to this > message by anyone else is unauthorized. If you are not the intended > recipient, any disclosure, copying, distribution or any action taken, > or omitted to be taken, in reliance on it is prohibited and may be > unlawful. If you have received this message in error, please delete > all electronic copies of this message (and the documents attached to > it, if any), destroy any hard copies you may have created and notify > me immediately by replying to this email. Thank you. > -------------- next part -------------- > BEGIN:VCARD > VERSION:2.1 > X-GWTYPE:USER > FN:Bitting, Angela > TEL;WORK:570-271-6844 > ORG:;Histology > EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu > N:Bitting;Angela > END:VCARD > > > ------------------------------ > > Message: 4 > Date: Mon, 21 Jul 2008 09:28:11 -0400 > From: "Patricia Karlisch" > Subject: [Histonet] Re: Histonet Digest, Vol 56, Issue 23 > To: > Message-ID: <488456AB.07F7.008C.0@hmc.psu.edu> > Content-Type: text/plain; charset=US-ASCII > > Jason, > I agree with you about Air Transportation and their specific rules > governing Air Transport, but hospitals have different requirements in > transporting hazardous materials within a hospital, from lab to lab > or clinic site to lab or by courier to lab. The secondary container > is for the protection of the transporter (hospital) and the specimen > integrity. > I may be reading the email wrong from Richard, but it sounded like > specimens were being transported in the equivalent of a zip lock bag. > If I was wrong I apologise. > Pat Karlisch > > Pat Karlisch > Supervisor, Histology, Pathology and Laboratory Medicine > Penn State Milton S. Hershey Medical Center > Mail Code H179 > Hershey, PA 17033 > Phone (717) 531-6072 > Fax: (717) 531- 7741 > email: pkarlisch@psu.edu > > *****E-Mail Confidentiality Notice***** > This message (including any attachments) contains information > intended for a specific individual(s) and purpose that may be > privileged, confidential or otherwise protected from disclosure > pursuant to applicable law. Any inappropriate use, distribution or > copying of the message is strictly prohibited and may subject you to > criminal or civil penalty. If you have received this transmission in > error, please reply to the sender indicating this error and delete > the transmission from your system immediately. > > >>>> 7/20/2008 1:04 PM >>> > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Formalin in plastic bags (Burrill, Jason) > 2. troubleshoot of positive controls for PKCalpha and eNOS > antibody (shazana hilda) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sat, 19 Jul 2008 13:38:10 -0400 > From: "Burrill, Jason" > Subject: [Histonet] RE: Formalin in plastic bags > To: , > Message-ID: > <1AD4E907E9B6F648AEF1B3A20A9B0E1E013B8E43@shr-exch2.na01.crl.com> > Content-Type: text/plain;charset="US-ASCII" > > Patricia you are correct about the classification of most formalin > solutions being hazardous for air transport but as it relates to 10% > neutral buffered formalin it does not apply. IATA regulations, which > govern air transports, clearly state in the Dangerous Goods Regulations > that any solution containing less than 10% formaldehyde is not > considered hazardous for air transport. And since 10% neutral buffered > formalin contains only 4% formaldehyde it is not considered a dangerous > good for transport. I will say that you should always check with your > commercial carrier (e.g. FedEx, UPS) before final classification of any > material that there is a question about as they may have stricter > requirements. > > > > Now if you are speaking about the transport of Patient Specimens as > defined by IATA, exempt human or animal, which formalin fixed tissue > typically falls into then what is required is a leak-proof primary > receptacle, leak-proof secondary packaging and a rigid outer packaging > with size dimensions of no less than 100 mm x 100 mm. > > > > And my final disclaimer will be that you should always check with your > EHS department to make sure that you are following your > institutional/company policies. > > > > Regards, > > Jason > > > > Jason Burrill > > Sr. Manager, Histology and Laboratory Safety > > Research Animal Diagnostic Services > > Charles River > > 251 Ballardvale St > > Wilmington, MA 01887 > > Office: 781-222-6152 > > Fax: 978-988-8793 > > jason.burrill@crl.com > > www.criver.com > > > > Accelerating Drug Development. Exactly. > > > > Notice - This email and any files transmitted with it are confidential > and may contain privileged and/or proprietary information. You must not > disclose this message to another party without Charles River's express > written consent. If you are not the intended recipient you must not > copy, distribute or use this email or the information contained in it > for any purpose other than to notify us. If you have received this > message in error, please notify Charles River immediately, and delete it > from your system. > > > > ------------------------------ > > > > Message: 2 > > Date: Fri, 18 Jul 2008 13:34:46 -0400 > > From: "Patricia Karlisch" > > Subject: [Histonet] Formalin in plastic bags > > To: > > Message-ID: <48809BF6.07F7.008C.0@hmc.psu.edu> > > Content-Type: text/plain; charset=US-ASCII > > > > Richard, > > There are standards for sending specimens that are in a hazardous > solution, like Formalin. Plastic bags are secondary containers and > should not be used to transport fluid. The specimens should be in a > secure plastic container that seals tightly and will not puncture > causing injury to others. > > Pat Karlisch > > > > Pat Karlisch > > Supervisor, Histology, Pathology and Laboratory Medicine > > Penn State Milton S. Hershey Medical Center > > Mail Code H179 > > Hershey, PA 17033 > > Phone (717) 531-6072 > > Fax: (717) 531- 7741 > > email: pkarlisch@psu.edu > > > > *****E-Mail Confidentiality Notice***** > > This message (including any attachments) contains information intended > for a specific individual(s) and purpose that may be privileged, > confidential or otherwise protected from disclosure pursuant to > applicable law. Any inappropriate use, distribution or copying of the > message is strictly prohibited and may subject you to criminal or civil > penalty. If you have received this transmission in error, please reply > to the sender indicating this error and delete the transmission from > your system immediately. > > > > > > > > ------------------------------ > > Message: 2 > Date: Sat, 19 Jul 2008 19:56:28 -0700 (PDT) > From: shazana hilda > Subject: [Histonet] troubleshoot of positive controls for PKCalpha and > eNOSantibody > To: histonet@lists.utsouthwestern.edu > Message-ID: <939284.12676.qm@web38806.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Dear Histonetters, > > I'm having difficulties to obtain positive staining of positive > control for PKCalpha (H-7:sc-8393) and NOS3(c-20:sc-654) antibody. > Both antibody were purchased from SantaCruz Biotechnology Inc.and > used for IHC.Below are the methods that i used: > > 1)PKCalpha > -incubation time: 1hour,room temperature (24-25 celcius) > -Antibody dilution: 1:50 > - Target Antigen Retrieval Buffer: Citrate Buffer, pH6 > -Device: microwave (3x at 600W for 5min) > -Detection method: polymeric method by LabVision > 2) NOS3@eNOS > -incubation time: 1hour,room temperature (24-25 celcius) > -Antibody dilution: 1:50 > - Target Antigen Retrieval Buffer: Citrate Buffer, pH6 > -Device: microwave (3x at 600W for 5min) > -Detection method: polymeric method by LabVision > > I've used normal rat kidney as suggested by few journals for positive > controls of both antibody. however my positive controls produced > negative staining. > > Your suggestion and quick response are highly appreciated > > Shazana Hilda Shamsuddin > MSc.of Molecular Pathology, > Dept. of Pathology, > School of Medical Sciences, > Universiti Sains Malaysia, > 16150 Kubang Kerian, > Kelantan, Malaysia. > Email: hilda_1075@yahoo.com > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 56, Issue 23 > **************************************** > > > ------------------------------ > > Message: 5 > Date: Mon, 21 Jul 2008 07:59:35 -0700 (PDT) > From: MARY JOHNSON > Subject: [Histonet] (no subject) > To: Histonet@lists.utsouthwestern.edu > Message-ID: <207645.64703.qm@web81605.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Is there something wrong with the histonet? I have not received > anything from you far a while > my e-mail is beingmary53@sbcglobal.net Please check my e-mail address. > Thank you Mary > > > ------------------------------ > > Message: 6 > Date: Mon, 21 Jul 2008 11:38:10 -0400 > From: "Karen Lapanowski" > Subject: [Histonet] Albumin Antibody > To: histonet@lists.utsouthwestern.edu > Message-ID: <488475220200002800009336@gwia2v.net.hfh.edu> > Content-Type: text/plain; charset=us-ascii > > Hello, > Can anyone recommend a supplier and/or protocol for staining albumin > in fixed 100 micron vibratome section of rat brain? > Thanks, > > Karen Lapanowski > Henry Ford Health System > Radiation Oncology > 3065 E & R > 313-916-9386 > > > ============================================================================== > CONFIDENTIALITY NOTICE: This email contains information from the > sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or > otherwise protected from disclosure. This email is intended for use > only by the person or entity to whom it is addressed. If you are not > the intended recipient, any use, disclosure, copying, distribution, > printing, or any action taken in reliance on the contents of this > email, is strictly prohibited. If you received this email in error, > please contact the sending party by reply email, delete the email > from your computer system and shred any paper copies. > > Note to Patients: There are a number of risks you should consider > before using e-mail to communicate with us. See our Privacy Policy > and Henry Ford My Health at www.henryford.com for more detailed > information. If you do not believe that our policy gives you the > privacy and security protection you need, do not send e-mail or > Internet communications to us. > > ============================================================================== > > > > > ------------------------------ > > Message: 7 > Date: Mon, 21 Jul 2008 11:45:43 -0400 > From: "Michael Mihalik" > Subject: RE: [Histonet] AP Cerner Copath vs Sunquest Copath vs others > To: "'Cristian Britos'" , "'Richard Cartun'" > , , > > Message-ID: <031001c8eb48$d5c57780$81506680$@com> > Content-Type: text/plain; charset="US-ASCII" > > Good day, > > I wanted to wait a few days before I responded to this request as I > represent a system vendor. I would love to talk to you about our system > and why your organization should consider us, but I suspect this would be > best dealt with via direct email. > > To the group, I pose this question: > > I try to be very conscientious of the fact that this method of communication > and interaction is intended to be primarily between histology personnel and > that vendors should be very respectful of that idiom. Do you think my offer > above is appropriate? Would you like to see our dialog? > > I'd appreciate any feedback that you can provide. > > Michael Mihalik > PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cristian > Britos > Sent: Thursday, July 17, 2008 3:26 PM > To: Richard Cartun; gu.lang@gmx.at; histonet@lists.utsouthwestern.edu > Subject: [Histonet] AP Cerner Copath vs Sunquest Copath vs others > > Would someone be willing to share their experiences about Sunquest > copath as their LIS system for pathology versus other systems? > Needless to say, we are looking for options.... (currently we have > cerner copath 3.0) > Thanks, > Cris > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 56, Issue 24 > **************************************** > Melissa R. Mazan, DVM, Diplomate ACVIM Associate Professor and Director of Equine Sports Medicine Department of Clinical Sciences Tufts Cumming School of Veterinary Medicine 200 Westborough Road North Grafton, MA 01536 Tel:508-839-5395 Fax:508-839-7922 email: melissa.mazan@tufts.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KPierce <@t> cancer-test.com Tue Jul 22 17:54:22 2008 From: KPierce <@t> cancer-test.com (Ken Pierce) Date: Tue Jul 22 17:54:26 2008 Subject: [Histonet] paraffin embedded formalin fixed human tissue In-Reply-To: <488606B5.50902@oncology.wisc.edu> References: <488606B5.50902@oncology.wisc.edu> Message-ID: <9380B79A09A6DD43884D977256FC12021DBE4E@fleming.mla.local> Joe, I may have human tissue blocks that you could use. Call me at 206-623-3814 or e-mail me at kpierce@cancer-test.com and give me some specifics of your needs. Ken Pierce, Med Lab Assoc, Seattle WA. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Hardin Sent: Tuesday, July 22, 2008 9:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] paraffin embedded formalin fixed human tissue Hi Everyone, I work in a core histology facility for researchers here at University of Wisconsin, Madison. I am looking for affordable paraffin embedded formalin fixed human tissue to use as control tissue for immunohistochemistry. The cheapest that I can find is >500.00/block. Does anyone know of a good source for this? From M.Donovan <@t> alfred.org.au Tue Jul 22 18:38:16 2008 From: M.Donovan <@t> alfred.org.au (Donovan, Mark) Date: Tue Jul 22 18:38:50 2008 Subject: [Histonet] SurPath PreCoat slides In-Reply-To: <20080722171922.A749E430AC0@SSICTmx01.baysidehealth.org.au> Message-ID: <1FDCAFF6F1DF8C428408C49E2EAA8EA56130E3@AC-VEX01.baysidehealth.intra> Dear All, We were recently provided with a pack of SurePath PreCoat slides (REF 080-10010-02) from TriPath Imaging (Becton Dickinson) to try in connection with their CytoRich cytology preservatives. We have been using the slides and reagents manually rather than on the BD PrepStain machine. My Cyto people tell me that even without the use of the CytoRich solution these slides seem considerably more "sticky" than our usual Menzel superfrost plus or polysine slides. They are finding significantly better cell attachment with Urines and fluids when used in the Cytospin. We are just curious to know what these slides are coated with. The slides display a visible drying line at the very bottom edge so appear to have been dipped in something. Our local BD rep was unable to enlighten. many thanks Mark Donovan Senior Scientist-Unit Manager Anatomical Pathology The Alfred, Melbourne, Australia. THIS E-MAIL IS CONFIDENTIAL. If you have received this e-mail in error, please notify us by return e-mail and delete the document. If you are not the intended recipient you are hereby notified that any disclosure, copying, distribution or taking any action in reliance on the contents of this information is strictly prohibited and may be unlawful. Bayside Health is not liable for the proper and complete transmission of the information contained in this communication or for any delay in its receipt. From ian.montgomery <@t> bio.gla.ac.uk Wed Jul 23 08:05:22 2008 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Wed Jul 23 08:05:29 2008 Subject: [Histonet] Fibre typing. Message-ID: <2CFA5F9766BD4C7CB91CC38F3CABBBE5@IBLS.GLA.AC.UK> I've been delving deep into the recesses of the brain for an answer to this, fibre typing on embalmed cadaver muscle. I'll have to use paraffin embedded muscle as cryostat sections are extremely difficult to obtain from embalmed material, at least here in Glasgow. Any and all suggestions appreciated. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. From Pierre.Larvaron <@t> medecine.unige.ch Wed Jul 23 09:40:07 2008 From: Pierre.Larvaron <@t> medecine.unige.ch (Larvaron Pierre) Date: Wed Jul 23 09:40:18 2008 Subject: [Histonet] rat brain: glycerol vs sucrose cryoprotection / Infiltration with Neg-50 at room temperature before freezing Message-ID: <488742C7.4090808@medecine.unige.ch> Dear all, I am looking for a protocol to enhance the quality of my frozen sections of fixed rat brain. I have two questions: - Has anyone used a 20% glycerol and 2%DMSO (in buffer or fixative) to cryoprotect tissue instead of 30% sucrose? (http://www.histosearch.com/histonet/Aug05A/Re.Histonetglycerolcryopr.html) - Has anyone used infiltration of tissue at room temperature with medium for embedding and cryosectionning (Neg-50 for example) before freezing? (http://www.ncbi.nlm.nih.gov/pubmed/8128478?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum) Thanks a lot. Pierre -- Dr. Pierre Larvaron Post-Doc Service du D?veloppement et de la Croissance D?partement de l'Enfant et de l'Adolescent Facult? de M?decine, Universit? de Gen?ve Avenue de la Roseraie 64 CH - 1205 Gen?ve tel: +41 22 382 4569 Fax: +41 22 347 5979 From GauchV <@t> mail.amc.edu Wed Jul 23 09:46:34 2008 From: GauchV <@t> mail.amc.edu (Vicki Gauch) Date: Wed Jul 23 09:47:25 2008 Subject: [Histonet] Tissue Carryover Message-ID: I apologize if this message is a repeat but I sent it two days ago and didn't see if appear on my Histonet messages so I was worried that it never went anywhere..In any case, here is my question.. As part of our Quality Assurance program we have been looking at our processes in Pathology and trying to identify areas of risk for our department and in the discussion the issue of potential tissue carryover in both the grossing and embedding procedures came up. I have been asked to inquire how other institutions handle this issue as far as prevention and what methods do you use to achieve that. I would appreciate any input on this as I have to report back to the group regarding any information I receive. Thank you in advance for your help, Vicki Gauch AMCH Albany, NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. From tshrobertson <@t> yahoo.com Wed Jul 23 10:21:40 2008 From: tshrobertson <@t> yahoo.com (Teisha Robertson) Date: Wed Jul 23 10:21:47 2008 Subject: [Histonet] vibratome questions Message-ID: <628297.44888.qm@web62513.mail.re1.yahoo.com> Good Morning All, ? We are getting a vibratome in our lab and I was wondering if anyone has a protocol for embedding fresh tissue of the mouse brain?? I heard that you can embed the tissue in agrase, if so what percentage and is that all I need?? Also, will a still be able to fix my tissue in formalin?? Any suggestions would greatly be appreciated. From tifei <@t> foxmail.com Wed Jul 23 10:24:28 2008 From: tifei <@t> foxmail.com (tf) Date: Wed Jul 23 10:24:49 2008 Subject: [Histonet] rat brain: glycerol vs sucrose cryoprotection /Infiltration with Neg-50 at room temperature before f References: <488742C7.4090808@medecine.unige.ch> Message-ID: <200807232324229344867@foxmail.com> UXVlc3Rpb24gMTogVGhhdCBpcyBvbmUgd2F5IHRvIHByZXNlcnZlIGJyYWluIHNlY3Rpb25zIHJh dGhlciBkZWh5ZHJhdGlvbi4NClF1ZXN0aW9uIDI6IEkgcmVhZCB0aGUgcGFwZXIgZnJvbSBKYXBh bmVzZSB3aXRoIGludGVyZXN0IGFuZCBJIHRoaW5rIHRoYXQncyByZWFsbHkgYSBncmVhdCB3YXN0 ZSBvZiBPLkMuVC4gDQogICAgICAgICAgICAgICAgIE15IGxhYm1hdGVzIHJlcG9ydGVkIHByZS1p bmZpbHRyYXRpb24gaW4gTy5DLlQgd2l0aG91dCBjaGFuZ2luZyBtZWRpdW0gYW5kIGdvIGZyZWV6 ZXIgYWZ0ZXIgMy02IGhvdXJzIGhlbHBzLiBIb3dldmVyIHRoYXQgbWF5IGRhbWFnZSB0aGUgYW50 aWdlbiBhbmQgeW91IGhhdmUgdG8gZG8gcmV0cml2YWwgZm9yIGltbXVub3N0YWluaW5nLiANCiAg ICAgICAgICAgICAgICAgDQoNCg0KMjAwOC0wNy0yMyANCg0KDQoNCnRmIA0KDQoNCg0Kt6K8/sjL o7ogTGFydmFyb24gUGllcnJlIA0Kt6LLzcqxvOSjuiAyMDA4LTA3LTIzICAyMjo1MjoxOSANCsrV vP7Iy6O6IGhpc3RvbmV0QGxpc3RzLnV0c291dGh3ZXN0ZXJuLmVkdSANCrOty82juiANCtb3zOKj uiBbSGlzdG9uZXRdIHJhdCBicmFpbjogZ2x5Y2Vyb2wgdnMgc3Vjcm9zZSBjcnlvcHJvdGVjdGlv biAvSW5maWx0cmF0aW9uIHdpdGggTmVnLTUwIGF0IHJvb20gdGVtcGVyYXR1cmUgYmVmb3JlIGYg DQogDQpEZWFyIGFsbCwNCkkgYW0gbG9va2luZyBmb3IgYSBwcm90b2NvbCB0byBlbmhhbmNlIHRo ZSBxdWFsaXR5IG9mIG15IGZyb3plbiBzZWN0aW9ucyANCm9mIGZpeGVkIHJhdCBicmFpbi4gSSBo YXZlIHR3byBxdWVzdGlvbnM6DQotIEhhcyBhbnlvbmUgdXNlZCBhIDIwJSBnbHljZXJvbCBhbmQg MiVETVNPIChpbiBidWZmZXIgb3IgZml4YXRpdmUpIHRvIA0KY3J5b3Byb3RlY3QgdGlzc3VlIGlu c3RlYWQgb2YgMzAlIHN1Y3Jvc2U/DQooaHR0cDovL3d3dy5oaXN0b3NlYXJjaC5jb20vaGlzdG9u ZXQvQXVnMDVBL1JlLkhpc3RvbmV0Z2x5Y2Vyb2xjcnlvcHJodG1sKQ0KLSBIYXMgYW55b25lIHVz ZWQgaW5maWx0cmF0aW9uIG9mIHRpc3N1ZSBhdCByb29tIHRlbXBlcmF0dXJlICB3aXRoIA0KbWVk aXVtIGZvciBlbWJlZGRpbmcgYW5kIGNyeW9zZWN0aW9ubmluZyAoTmVnLTUwIGZvciBleGFtcGxl KSBiZWZvcmUgDQpmcmVlemluZz8NCihodHRwOi8vd3d3Lm5jYmkubmxtLm5paC5nb3YvcHVibWVk LzgxMjg0Nzg/b3JkaW5hbHBvcz0xJml0b29sPUVudHJlelN5c3RlbTIuUEVudHJlei5QdWJtZWQu UHVibWVkX1Jlc3VsdHNQYW5lbC5QdWJtZWRfUlZEb2NTdW0pDQpUaGFua3MgYSBsb3QuDQpQaWVy cmUNCi0tIA0KRHIuIFBpZXJyZSBMYXJ2YXJvbg0KUG9zdC1Eb2MNClNlcnZpY2UgZHUgROl2ZWxv cHBlbWVudCBldCBkZSBsYSBDcm9pc3NhbmNlDQpE6XBhcnRlbWVudCBkZSBsJ0VuZmFudCBldCBk ZSBsJ0Fkb2xlc2NlbnQNCkZhY3VsdD9kZSBN6WRlY2luZSwgVW5pdmVyc2l0P2RlIEdlbuh2ZQ0K QXZlbnVlIGRlIGxhIFJvc2VyYWllIDY0DQpDSCAtIDEyMDUgR2Vu6HZlDQp0ZWw6ICs0MSAyMiAz ODIgNDU2OQ0KRmF4OiArNDEgMjIgMzQ3IDU5NzkNCl9fX19fX19fX19fX19fX19fX19fX19fX19f X19fX19fX19fX19fX19fX19fX19fDQpIaXN0b25ldCBtYWlsaW5nIGxpc3QNCkhpc3RvbmV0QGxp c3RzLnV0c291dGh3ZXN0ZXJuLmVkdQ0K From juditw <@t> u.washington.edu Wed Jul 23 11:13:21 2008 From: juditw <@t> u.washington.edu (Judith L. Williams) Date: Wed Jul 23 11:13:25 2008 Subject: [Histonet] vibratome questions In-Reply-To: <628297.44888.qm@web62513.mail.re1.yahoo.com> Message-ID: Hi- I have a procedure for embedding mouse embryos and whole hearts in acrylamide for vibratome sectioning. If you are interested, I can send it over to you under separate email. Judy Williams Comparative Medicine U of WAshington On Wed, 23 Jul 2008, Teisha Robertson wrote: > Good Morning All, > ? > We are getting a vibratome in our lab and I was wondering if anyone has a protocol for embedding fresh tissue of the mouse brain?? I heard that you can embed the tissue in agrase, if so what percentage and is that all I need?? Also, will a still be able to fix my tissue in formalin?? Any suggestions would greatly be appreciated. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Judith Williams, PhD, HT(ASCP) Research Scientist Department of Comparative Medicine University of Washington Seattle, WA 98195 From burch007 <@t> mc.duke.edu Wed Jul 23 11:23:52 2008 From: burch007 <@t> mc.duke.edu (James L Burchette) Date: Wed Jul 23 11:23:59 2008 Subject: [Histonet] Tissue Whole Mount Info Request Message-ID: Dear Colleagues, I have been asked to gather start to finish information for human prostate whole mount slide preparations. Where can I obtain large tissue processing baskets, 4x4 whole mount slides and cover glass? I seem to recall a brain research company that listed these products in their catalog. Does anyone have a favorite sliding microtome to recommend? Any information would be appreciated. Best Regards, Jim Burchette Duke University Medical Center Immunopathology Lab From tifei <@t> foxmail.com Wed Jul 23 11:29:16 2008 From: tifei <@t> foxmail.com (tf) Date: Wed Jul 23 11:29:38 2008 Subject: [Histonet] vibratome questions References: <628297.44888.qm@web62513.mail.re1.yahoo.com> Message-ID: <200807240029109908923@foxmail.com> MS4geW91IGNhbiBwcmVmdXNlIGFuZCBkZWh5ZHJhdGUgdGhlIHRpc3N1ZSBiZWZvcmUgc2VjdGlv biBpdCBvbiB2aWJyYXRvbWUNCjIuIGFncmFzZSB3aWxsIGhlbHANCg0KDQoyMDA4LTA3LTI0IA0K DQoNCg0KdGYgDQoNCg0KDQq3orz+yMujuiBUZWlzaGEgUm9iZXJ0c29uIA0Kt6LLzcqxvOSjuiAy MDA4LTA3LTIzICAyMzozMDo1NSANCsrVvP7Iy6O6IGggbiANCrOty82juiANCtb3zOKjuiBbSGlz dG9uZXRdIHZpYnJhdG9tZSBxdWVzdGlvbnMgDQogDQpHb29kIE1vcm5pbmcgQWxsLA0KPw0KV2Ug YXJlIGdldHRpbmcgYSB2aWJyYXRvbWUgaW4gb3VyIGxhYiBhbmQgSSB3YXMgd29uZGVyaW5nIGlm IGFueW9uZSBoYXMgYSBwcm90b2NvbCBmb3IgZW1iZWRkaW5nIGZyZXNoIHRpc3N1ZSBvZiB0aGUg bW91c2UgYnJhaW4/P0kgaGVhcmQgdGhhdCB5b3UgY2FuIGVtYmVkIHRoZSB0aXNzdWUgaW4gYWdy YXNlLCBpZiBzbyB3aGF0IHBlcmNlbnRhZ2UgYW5kIGlzIHRoYXQgYWxsIEkgbmVlZD8/QWxzbywg d2lsbCBhIHN0aWxsIGJlIGFibGUgdG8gZml4IG15IHRpc3N1ZSBpbiBmb3JtYWxpbj8/QW55IHN1 Z2dlc3Rpb25zIHdvdWxkIGdyZWF0bHkgYmUgYXBwcmVjaWF0ZWQuDQogICAgICANCl9fX19fX19f X19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fX19fDQpIaXN0b25ldCBtYWlsaW5n IGxpc3QNCkhpc3RvbmV0QGxpc3RzLnV0c291dGh3ZXN0ZXJuLmVkdQ0K From fong <@t> zoology.ubc.ca Wed Jul 23 12:29:36 2008 From: fong <@t> zoology.ubc.ca (Angelina Fong) Date: Wed Jul 23 12:30:11 2008 Subject: [Histonet] Preserving brain tissue for transport Message-ID: <48876A80.6040502@zoology.ubc.ca> Hi everyone, What would be the best medium to preserve and transport small pieces of brain tissue in for a prolonged period of time without ruining the tissue or the antigenicity? To give you some background to this question - I will be travelling to a lab in Australia to do some experiment and at the end of each experiment, I would like to fix and preserve the tissue for subsequent whole mount immunohistochemistry or ISH. However, I need to bring the tissue back to my lab here in Canada to process. So I will need to fix and store the tissue for an indefinite period of time. I am wondering what would be the solution to store the tissue in for that period of time. Previously, I have stored fixed brain tissue from adult rats in PBS for weeks. However, this lot of tissue will be very small (neonates and embryos), so I am concerned the tissue will become 'unfixed'. But if I leave it in the fix, I'm concerned it will become overfixed ... Does anyone have any suggestions for what I should do? Thanks in advance. Angelina From boneslides <@t> aol.com Wed Jul 23 12:36:29 2008 From: boneslides <@t> aol.com (boneslides@aol.com) Date: Wed Jul 23 12:36:52 2008 Subject: [Histonet] Please help settle debate!! Message-ID: <8CABB01C66D3FA2-1280-2880@mblk-d39.sysops.aol.com> Hello Histonetters! Please help settle a debate raging downstairs...how many microns are in a 3mm. thick piece of tissue?? I look forward to your responses!! Thanks, Diane Cleveland Clinic Cleveland, Ohio From asmith <@t> mail.barry.edu Wed Jul 23 12:44:14 2008 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Wed Jul 23 12:45:41 2008 Subject: [Histonet] Please help settle debate!! In-Reply-To: <8CABB01C66D3FA2-1280-2880@mblk-d39.sysops.aol.com> References: <8CABB01C66D3FA2-1280-2880@mblk-d39.sysops.aol.com> Message-ID: 1 micrometer = 0.001 mm Thus 3 mm = 3000 microns -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of boneslides@aol.com Sent: Wednesday, July 23, 2008 1:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Please help settle debate!! Hello Histonetters! Please help settle a debate raging downstairs...how many microns are in a 3mm. thick piece of tissue?? I look forward to your responses!! Thanks, Diane Cleveland Clinic Cleveland, Ohio _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jennifer.bull <@t> northwestpathology.com Wed Jul 23 12:47:09 2008 From: jennifer.bull <@t> northwestpathology.com (Bull, Jennifer L.) Date: Wed Jul 23 12:47:15 2008 Subject: [Histonet] Whole Mount Block Storage Message-ID: <85760CECEC18444BB95F26D5E88DAEAA21C1C1F3BF@hinet2.hinet.org> A few months ago, we began cutting Whole Mount blocks. I am using the Super Cassettes from Surgipath. I have been able to find all the supplies for whole mounts EXCEPT for a means to store the whole mount blocks. They do not fit in the normal block storage boxes. I would appreciate any feedback or leads from those of you that are already storing these monster blocks. Thanks! Jenny Bull Histology Supervisor Northwest Pathology jennifer.bull@northwestpathology.com From Jackie.O'Connor <@t> abbott.com Wed Jul 23 12:49:32 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed Jul 23 12:49:53 2008 Subject: [Histonet] Please help settle debate!! In-Reply-To: <8CABB01C66D3FA2-1280-2880@mblk-d39.sysops.aol.com> Message-ID: . 1,000 microns to a milimeter, so your answer is 3,000. Now, here's a real question - if a chicken and a half can lay an egg and a half in a day and a half, how long would it take a grasshopper with a wooden leg to kick all the seeds out of a dill pickle? boneslides@aol.com Sent by: histonet-bounces@lists.utsouthwestern.edu 07/23/2008 12:36 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Please help settle debate!! Hello Histonetters! Please help settle a debate raging downstairs...how many microns are in a 3mm. thick piece of tissue?? I look forward to your responses!! Thanks, Diane Cleveland Clinic Cleveland, Ohio _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Jul 23 12:42:28 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Jul 23 12:52:46 2008 Subject: [Histonet] Please help settle debate!! In-Reply-To: <8CABB01C66D3FA2-1280-2880@mblk-d39.sysops.aol.com> References: <8CABB01C66D3FA2-1280-2880@mblk-d39.sysops.aol.com> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A7F23D67@LTA3VS011.ees.hhs.gov> 1 millimeter = 1 000 microns so just multiply by 3: 3 millimeters = 3000 microns Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of boneslides@aol.com Sent: Wednesday, July 23, 2008 1:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Please help settle debate!! Hello Histonetters! Please help settle a debate raging downstairs...how many microns are in a 3mm. thick piece of tissue?? I look forward to your responses!! Thanks, Diane Cleveland Clinic Cleveland, Ohio _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Jul 23 12:52:28 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Jul 23 12:54:11 2008 Subject: [Histonet] Please help settle debate!! In-Reply-To: References: <8CABB01C66D3FA2-1280-2880@mblk-d39.sysops.aol.com> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A7F23D68@LTA3VS011.ees.hhs.gov> How big is the dill pickle and does the grasshopper still have a good working leg as well as the wooden one? Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Wednesday, July 23, 2008 1:50 PM To: boneslides@aol.com Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Please help settle debate!! . 1,000 microns to a milimeter, so your answer is 3,000. Now, here's a real question - if a chicken and a half can lay an egg and a half in a day and a half, how long would it take a grasshopper with a wooden leg to kick all the seeds out of a dill pickle? boneslides@aol.com Sent by: histonet-bounces@lists.utsouthwestern.edu 07/23/2008 12:36 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Please help settle debate!! Hello Histonetters! Please help settle a debate raging downstairs...how many microns are in a 3mm. thick piece of tissue?? I look forward to your responses!! Thanks, Diane Cleveland Clinic Cleveland, Ohio _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carrolpb <@t> umdnj.edu Wed Jul 23 12:57:56 2008 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Wed Jul 23 12:58:24 2008 Subject: [Histonet] Please help settle debate!! In-Reply-To: <8CABB01C66D3FA2-1280-2880@mblk-d39.sysops.aol.com> References: <8CABB01C66D3FA2-1280-2880@mblk-d39.sysops.aol.com> Message-ID: <48877124.5090405@umdnj.edu> > Please help settle a debate raging downstairs...how many microns are in a 3mm. thick piece of tissue? just out of curiosity... how does one engage in a raging debate about something like this? From Jackie.O'Connor <@t> abbott.com Wed Jul 23 13:29:13 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed Jul 23 13:29:33 2008 Subject: [Histonet] Please help settle debate!! In-Reply-To: <48877124.5090405@umdnj.edu> Message-ID: Must be PhD's. Peter Carroll Sent by: histonet-bounces@lists.utsouthwestern.edu 07/23/2008 12:57 PM To cc histonet@lists.utsouthwestern.edu Subject Re: [Histonet] Please help settle debate!! > Please help settle a debate raging downstairs...how many microns are in a 3mm. thick piece of tissue? just out of curiosity... how does one engage in a raging debate about something like this? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Jul 23 14:00:30 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Jul 23 14:00:41 2008 Subject: [Histonet] Please help settle debate!! In-Reply-To: References: <8CABB01C66D3FA2-1280-2880@mblk-d39.sysops.aol.com> <1CE1847DFEA0A647B1CCDE4108EA60A7F23D68@LTA3VS011.ees.hhs.gov> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A7F23D6A@LTA3VS011.ees.hhs.gov> And we all know how much wood a wood chuck chucks, right? Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: MaryAnn Dixon [mailto:DixonM@vetmed.ufl.edu] Sent: Wednesday, July 23, 2008 2:37 PM To: Bartlett, Jeanine (CDC/CCID/NCZVED) Subject: RE: [Histonet] Please help settle debate!! Also, is the wood of the wooden leg of the grasshopper oak or cherry? Is this grasshopper cousin to the wood chuck who chucks wood? MaryAnn Dixon BS Biological Scientist Anatomic Pathology UF Veterinary Medical Center (352) 392-2235 Ext. 4517 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Wednesday, July 23, 2008 1:52 PM To: Jackie M O'Connor; boneslides@aol.com Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Please help settle debate!! How big is the dill pickle and does the grasshopper still have a good working leg as well as the wooden one? Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Wednesday, July 23, 2008 1:50 PM To: boneslides@aol.com Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Please help settle debate!! . 1,000 microns to a milimeter, so your answer is 3,000. Now, here's a real question - if a chicken and a half can lay an egg and a half in a day and a half, how long would it take a grasshopper with a wooden leg to kick all the seeds out of a dill pickle? boneslides@aol.com Sent by: histonet-bounces@lists.utsouthwestern.edu 07/23/2008 12:36 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Please help settle debate!! Hello Histonetters! Please help settle a debate raging downstairs...how many microns are in a 3mm. thick piece of tissue?? I look forward to your responses!! Thanks, Diane Cleveland Clinic Cleveland, Ohio _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Jul 23 14:00:47 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Jul 23 14:01:08 2008 Subject: [Histonet] Please help settle debate!! In-Reply-To: References: <48877124.5090405@umdnj.edu> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A7F23D6B@LTA3VS011.ees.hhs.gov> Hah! Good one! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Wednesday, July 23, 2008 2:29 PM To: Peter Carroll Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Please help settle debate!! Must be PhD's. Peter Carroll Sent by: histonet-bounces@lists.utsouthwestern.edu 07/23/2008 12:57 PM To cc histonet@lists.utsouthwestern.edu Subject Re: [Histonet] Please help settle debate!! > Please help settle a debate raging downstairs...how many microns are in a 3mm. thick piece of tissue? just out of curiosity... how does one engage in a raging debate about something like this? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Wed Jul 23 14:01:21 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Jul 23 14:01:25 2008 Subject: [Histonet] Please help settle debate!! In-Reply-To: References: <8CABB01C66D3FA2-1280-2880@mblk-d39.sysops.aol.com> Message-ID: > > On Wed, Jul 23, 2008 at 1:49 PM, Jackie M O'Connor < > Jackie.O'Connor@abbott.com > wrote: > >> . 1,000 microns to a milimeter, so your answer is 3,000. >> >> Now, here's a real question - if a chicken and a half can lay an egg and a >> half in a day and a half, how long would it take a grasshopper with a >> wooden leg to kick all the seeds out of a dill pickle? >> >> > > 42. Dur-hey. Emily -- An overcivilized people grow complacent and careless and leave the door open for a tribe of fanatical savages, through a mixture of luck, treachery, and the foulest inhumanity, to usurp their place for a few years. -Richard Adams, "Shardik", 1974 From lblazek <@t> digestivespecialists.com Wed Jul 23 14:17:07 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Jul 23 14:09:56 2008 Subject: [Histonet] Friday Message-ID: <5A2BD13465E061429D6455C8D6B40E390B77E2E3@IBMB7Exchange.digestivespecialists.com> Hey! Is it Friday!!!!!???? Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com From JWeems <@t> sjha.org Wed Jul 23 14:10:15 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Jul 23 14:10:36 2008 Subject: [Histonet] Please help settle debate!! In-Reply-To: References: <8CABB01C66D3FA2-1280-2880@mblk-d39.sysops.aol.com> Message-ID: <982A0A9461F9BF438C7B19A6E425A383434AB0@ITSSSXM01V6.one.ads.che.org> So much fun and it's only Wednesday? Thanks!!!! Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From rjbuesa <@t> yahoo.com Wed Jul 23 14:21:59 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jul 23 14:22:04 2008 Subject: [Histonet] Please help settle debate!! In-Reply-To: <48877124.5090405@umdnj.edu> Message-ID: <127016.21493.qm@web65713.mail.ac4.yahoo.com> There are 1,000 micrometers in 1 mm so, there are 3,000 ?m in 3 mm ? Ren? J. --- On Wed, 7/23/08, Peter Carroll wrote: From: Peter Carroll Subject: Re: [Histonet] Please help settle debate!! To: Cc: histonet@lists.utsouthwestern.edu Date: Wednesday, July 23, 2008, 1:57 PM > Please help settle a debate raging downstairs...how many microns are in a 3mm. thick piece of tissue? just out of curiosity... how does one engage in a raging debate about something like this? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Jul 23 14:24:50 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jul 23 14:24:55 2008 Subject: [Histonet] Tissue Whole Mount Info Request In-Reply-To: Message-ID: <605892.76637.qm@web65706.mail.ac4.yahoo.com> Almost any lab provider (like Fisher) now carry those cassettes, molds and slides/coverslips. Buy a Leica sledge microtome. Ren? J. --- On Wed, 7/23/08, James L Burchette wrote: From: James L Burchette Subject: [Histonet] Tissue Whole Mount Info Request To: histonet@lists.utsouthwestern.edu Date: Wednesday, July 23, 2008, 12:23 PM Dear Colleagues, I have been asked to gather start to finish information for human prostate whole mount slide preparations. Where can I obtain large tissue processing baskets, 4x4 whole mount slides and cover glass? I seem to recall a brain research company that listed these products in their catalog. Does anyone have a favorite sliding microtome to recommend? Any information would be appreciated. Best Regards, Jim Burchette Duke University Medical Center Immunopathology Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pjfnefro <@t> duke.edu Wed Jul 23 14:27:01 2008 From: pjfnefro <@t> duke.edu (Pat Flannery) Date: Wed Jul 23 14:27:05 2008 Subject: [Histonet] Friday In-Reply-To: <5A2BD13465E061429D6455C8D6B40E390B77E2E3@IBMB7Exchange.digestivespecialists.com> References: <5A2BD13465E061429D6455C8D6B40E390B77E2E3@IBMB7Exchange.digestivespecialists.com> Message-ID: Wishful thinking, Linda, just wishful thinking. Maybe it *should* be Friday by now for some of us. There's nothing wrong with Friday jokes on Wednesday; gives us a light at the end of the tunnel (and I don't mean another train). -Pat Flannery Duke Med Center On Jul 23, 2008, at 3:17 PM, Blazek, Linda wrote: > Hey! Is it Friday!!!!!???? > > Linda Blazek HT (ASCP) > Manager/Supervisor > GI Pathology of Dayton > 7415 Brandt Pike > Huber Heights, OH 45424 > Phone: (937) 293-4424 ext 7118 > Email: lblazek@digestivespecialists.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bakevictoria <@t> gmail.com Wed Jul 23 14:40:58 2008 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Wed Jul 23 14:41:03 2008 Subject: [Histonet] Please help settle debate!! In-Reply-To: <127016.21493.qm@web65713.mail.ac4.yahoo.com> References: <48877124.5090405@umdnj.edu> <127016.21493.qm@web65713.mail.ac4.yahoo.com> Message-ID: <4f016b690807231240m6cc81460la3da665267422a0@mail.gmail.com> How much does anyone want to bet this will NEVER be a question in theTrivial Pursuits! I agree with Jackie - PhD's remember what that means right? 'Piled high and deep' On 7/23/08, Rene J Buesa wrote: > There are 1,000 micrometers in 1 mm so, there are 3,000 ?m in 3 mm > > Ren? J. > > --- On Wed, 7/23/08, Peter Carroll wrote: > > From: Peter Carroll > Subject: Re: [Histonet] Please help settle debate!! > To: > Cc: histonet@lists.utsouthwestern.edu > Date: Wednesday, July 23, 2008, 1:57 PM > > > Please help settle a debate raging downstairs...how many microns are > in a 3mm. thick piece of tissue? > > just out of curiosity... how does one engage in a raging debate about > something like this? > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lblazek <@t> digestivespecialists.com Wed Jul 23 14:51:38 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Jul 23 14:44:08 2008 Subject: [Histonet] Friday In-Reply-To: References: <5A2BD13465E061429D6455C8D6B40E390B77E2E3@IBMB7Exchange.digestivespecialists.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E390B77E2E6@IBMB7Exchange.digestivespecialists.com> Ahhh oh well. I was really hoping it was Friday. Alas it's only hump day. But that's a good thing too! Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat Flannery Sent: Wednesday, July 23, 2008 3:27 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Friday Wishful thinking, Linda, just wishful thinking. Maybe it *should* be Friday by now for some of us. There's nothing wrong with Friday jokes on Wednesday; gives us a light at the end of the tunnel (and I don't mean another train). -Pat Flannery Duke Med Center On Jul 23, 2008, at 3:17 PM, Blazek, Linda wrote: > Hey! Is it Friday!!!!!???? > > Linda Blazek HT (ASCP) > Manager/Supervisor > GI Pathology of Dayton > 7415 Brandt Pike > Huber Heights, OH 45424 > Phone: (937) 293-4424 ext 7118 > Email: lblazek@digestivespecialists.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Wed Jul 23 14:45:49 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed Jul 23 14:46:10 2008 Subject: [Histonet] Please help settle debate!! In-Reply-To: <4f016b690807231240m6cc81460la3da665267422a0@mail.gmail.com> Message-ID: I thought it was Post Hole Digger? "Victoria Baker" Sent by: histonet-bounces@lists.utsouthwestern.edu 07/23/2008 02:40 PM To rjbuesa@yahoo.com cc histonet@lists.utsouthwestern.edu Subject Re: [Histonet] Please help settle debate!! How much does anyone want to bet this will NEVER be a question in theTrivial Pursuits! I agree with Jackie - PhD's remember what that means right? 'Piled high and deep' On 7/23/08, Rene J Buesa wrote: > There are 1,000 micrometers in 1 mm so, there are 3,000 ?m in 3 mm > > Ren? J. > > --- On Wed, 7/23/08, Peter Carroll wrote: > > From: Peter Carroll > Subject: Re: [Histonet] Please help settle debate!! > To: > Cc: histonet@lists.utsouthwestern.edu > Date: Wednesday, July 23, 2008, 1:57 PM > > > Please help settle a debate raging downstairs...how many microns are > in a 3mm. thick piece of tissue? > > just out of curiosity... how does one engage in a raging debate about > something like this? > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RossS <@t> BaylorHealth.edu Wed Jul 23 14:49:02 2008 From: RossS <@t> BaylorHealth.edu (Stapf, Ross) Date: Wed Jul 23 14:49:09 2008 Subject: [Histonet] Please help settle debate!! In-Reply-To: <4f016b690807231240m6cc81460la3da665267422a0@mail.gmail.com> Message-ID: And of course whoever is asking this question will be expecting exactly 1000 3 micron slides to be produced from this 3mm thick piece of tissue. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax RossS@baylorhealth.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Wednesday, July 23, 2008 2:41 PM To: rjbuesa@yahoo.com Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Please help settle debate!! How much does anyone want to bet this will NEVER be a question in theTrivial Pursuits! I agree with Jackie - PhD's remember what that means right? 'Piled high and deep' On 7/23/08, Rene J Buesa wrote: > There are 1,000 micrometers in 1 mm so, there are 3,000 ?m in 3 mm > > Ren? J. > > --- On Wed, 7/23/08, Peter Carroll wrote: > > From: Peter Carroll > Subject: Re: [Histonet] Please help settle debate!! > To: > Cc: histonet@lists.utsouthwestern.edu > Date: Wednesday, July 23, 2008, 1:57 PM > > > Please help settle a debate raging downstairs...how many microns are > in a 3mm. thick piece of tissue? > > just out of curiosity... how does one engage in a raging debate about > something like this? > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Baylor Health Care System immediately at 1-866-402-1661 or via e-mail at privacy@baylorhealth.edu. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From juditw <@t> u.washington.edu Wed Jul 23 14:56:56 2008 From: juditw <@t> u.washington.edu (Judith L. Williams) Date: Wed Jul 23 14:57:00 2008 Subject: [Histonet] Please help settle debate!! In-Reply-To: Message-ID: hey..... you making fun of one of my presents when I go my PHD??? yep- a red neck friend just had to give me a brand new - RED- post hole digger. hmmm.... what was the question again? :-) rock on hump day Judy On Wed, 23 Jul 2008, Jackie M O'Connor wrote: > I thought it was Post Hole Digger? > > > > > "Victoria Baker" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 07/23/2008 02:40 PM > > To > rjbuesa@yahoo.com > cc > histonet@lists.utsouthwestern.edu > Subject > Re: [Histonet] Please help settle debate!! > > > > > > > How much does anyone want to bet this will NEVER be a question in > theTrivial Pursuits! > > I agree with Jackie - PhD's remember what that means right? 'Piled > high and deep' > > On 7/23/08, Rene J Buesa wrote: >> There are 1,000 micrometers in 1 mm so, there are 3,000 ?m in 3 mm >> >> Ren? J. >> >> --- On Wed, 7/23/08, Peter Carroll wrote: >> >> From: Peter Carroll >> Subject: Re: [Histonet] Please help settle debate!! >> To: >> Cc: histonet@lists.utsouthwestern.edu >> Date: Wednesday, July 23, 2008, 1:57 PM >> >>> Please help settle a debate raging downstairs...how many microns are >> in a 3mm. thick piece of tissue? >> >> just out of curiosity... how does one engage in a raging debate about >> something like this? >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Judith Williams, PhD, HT(ASCP) Research Scientist Department of Comparative Medicine University of Washington Seattle, WA 98195 From bakevictoria <@t> gmail.com Wed Jul 23 15:21:49 2008 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Wed Jul 23 15:21:55 2008 Subject: [Histonet] Please help settle debate!! In-Reply-To: References: Message-ID: <4f016b690807231321j17d839aeyda179a006faca113@mail.gmail.com> The 'post hole digger' is the one who has to clean up what the 'piled high and deep' one left behind. Just having fun. On 7/23/08, Judith L. Williams wrote: > hey..... you making fun of one of my presents when I go my PHD??? yep- a red > neck friend just had to give me a brand new - RED- post hole digger. > hmmm.... what was the question again? :-) > rock on hump day > Judy > > > > On Wed, 23 Jul 2008, Jackie M O'Connor wrote: > > > I thought it was Post Hole Digger? > > > > > > > > > > "Victoria Baker" > > Sent by: histonet-bounces@lists.utsouthwestern.edu > > 07/23/2008 02:40 PM > > > > To > > rjbuesa@yahoo.com > > cc > > histonet@lists.utsouthwestern.edu > > Subject > > Re: [Histonet] Please help settle debate!! > > > > > > > > > > > > > > How much does anyone want to bet this will NEVER be a question in > > theTrivial Pursuits! > > > > I agree with Jackie - PhD's remember what that means right? 'Piled > > high and deep' > > > > On 7/23/08, Rene J Buesa wrote: > > > > > There are 1,000 micrometers in 1 mm so, there are 3,000 ?m in 3 mm > > > > > > Ren? J. > > > > > > --- On Wed, 7/23/08, Peter Carroll wrote: > > > > > > From: Peter Carroll > > > Subject: Re: [Histonet] Please help settle debate!! > > > To: > > > Cc: histonet@lists.utsouthwestern.edu > > > Date: Wednesday, July 23, 2008, 1:57 PM > > > > > > > > > > Please help settle a debate raging downstairs...how many microns are > > > > > > > in a 3mm. thick piece of tissue? > > > > > > just out of curiosity... how does one engage in a raging debate about > > > something like this? > > > > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > Judith Williams, PhD, HT(ASCP) > Research Scientist > Department of Comparative Medicine > University of Washington > Seattle, WA 98195 > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From wdesalvo.cac <@t> hotmail.com Wed Jul 23 17:01:31 2008 From: wdesalvo.cac <@t> hotmail.com (WILLIAM DESALVO) Date: Wed Jul 23 17:01:37 2008 Subject: [Histonet] Tissue Carryover In-Reply-To: References: Message-ID: I suggest instituting simple QA measures to reduce risk at both tasks. At the Gross bench, one specimen on the bench at a time and clean up between specimens. Clean-up is new paper or rinsing of the gross cutting board and rinsing of all instruments. At the embedding bench, one cassette on the work area at a time and clean-up after each cassette and wipe down of forceps before returning to the warming area. These methods must be in the procedure/protocol for the task and more importantly develop a monitoring/review process of staff. Last, but most importantly, document any defects created (tissue contamination), require daily feedback from pathologist for all quality issues and then provide timely review and resolution for all defects back to the individual employees involved in the task and creating the defect. Unfortunately, these measures will not prevent tissue contamination during processing and that, I have found, is a more likely culprit in the process, especially if you are seeing cells or groups of cells and not chunks of tissue. William DeSalvo BS, HTL (ASCP) > Date: Wed, 23 Jul 2008 10:46:34 -0400> From: GauchV@mail.amc.edu> To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu> Subject: [Histonet] Tissue Carryover> > I apologize if this message is a repeat but I sent it two days ago and> didn't see if appear on my Histonet messages so I was worried that it> never went anywhere..In any case, here is my question..> As part of our Quality Assurance program we have been looking at our> processes in Pathology and trying to identify areas of risk for our> department and in the discussion the issue of potential tissue> carryover in both the grossing and embedding procedures came up. I have> been asked to inquire how other institutions handle this issue as far as> prevention and what methods do you use to achieve that. I would> appreciate any input on this as I have to report back to the group> regarding any information I receive. > Thank you in advance for your help,> Vicki Gauch> AMCH> Albany, NY> > -----------------------------------------> CONFIDENTIALITY NOTICE: This email and any attachments may contain> confidential information that is protected by law and is for the> sole use of the individuals or entities to which it is addressed.> If you are not the intended recipient, please notify the sender by> replying to this email and destroying all copies of the> communication and attachments. Further use, disclosure, copying,> distribution of, or reliance upon the contents of this email and> attachments is strictly prohibited. To contact Albany Medical> Center, or for a copy of our privacy practices, please visit us on> the Internet at www.amc.edu.> > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Use video conversation to talk face-to-face with Windows Live Messenger. http://www.windowslive.com/messenger/connect_your_way.html?ocid=TXT_TAGLM_WL_Refresh_messenger_video_072008 From CIngles <@t> uwhealth.org Wed Jul 23 17:03:00 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Wed Jul 23 17:03:05 2008 Subject: [Histonet] Friday In-Reply-To: References: <5A2BD13465E061429D6455C8D6B40E390B77E2E3@IBMB7Exchange.digestivespecialists.com>, Message-ID: <8408625A-12FB-4ADA-8E2E-D477FF1B1396@mimectl> I don't know, that's all I seem to keep finding at the end of my tunnels. Claire There's nothing wrong with Friday jokes on Wednesday; gives us a light at the end of the tunnel (and I don't mean another train). -Pat Flannery Duke Med Center From jnocito <@t> satx.rr.com Wed Jul 23 17:06:10 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Jul 23 17:05:37 2008 Subject: [Histonet] Friday References: <5A2BD13465E061429D6455C8D6B40E390B77E2E3@IBMB7Exchange.digestivespecialists.com> Message-ID: <009101c8ed10$50e73b10$0302a8c0@yourxhtr8hvc4p> I don't know about Friday, but it's 5 o'clock somewhere. ----- Original Message ----- From: "Blazek, Linda" To: Cc: "'Breeden, Sara'" Sent: Wednesday, July 23, 2008 2:17 PM Subject: [Histonet] Friday Hey! Is it Friday!!!!!???? Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Wed Jul 23 17:06:37 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Wed Jul 23 17:06:42 2008 Subject: [Histonet] Please help settle debate!! In-Reply-To: References: <4f016b690807231240m6cc81460la3da665267422a0@mail.gmail.com>, Message-ID: <244A31EE-7FDB-465F-A779-F98CB4260464@mimectl> Only if there is only one section on a slide... But they'd still count the number of sections. Sorry, clinic politics are REALLY stressing me out today. I guess I will have to visit my buddy Jim Beam after work today. Claire And of course whoever is asking this question will be expecting exactly 1000 3 micron slides to be produced from this 3mm thick piece of tissue. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax RossS@baylorhealth.edu From myl__28 <@t> hotmail.com Wed Jul 23 17:31:29 2008 From: myl__28 <@t> hotmail.com (=?iso-8859-1?Q?Myl=E8ne_de_Champlain?=) Date: Wed Jul 23 17:31:33 2008 Subject: [Histonet] Filter paper Message-ID: Hi, To filter blue celestin, is what I have to use a filter paper number 1 or 4? Thank! Myl?ne de Champlain _________________________________________________________________ From AnthonyH <@t> chw.edu.au Wed Jul 23 18:29:09 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Jul 23 18:29:28 2008 Subject: [Histonet] Fibre typing. In-Reply-To: <2CFA5F9766BD4C7CB91CC38F3CABBBE5@IBLS.GLA.AC.UK> Message-ID: Try immunohistochemistry. There are fast and slow antbodies available Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian Montgomery Sent: Wednesday, 23 July 2008 11:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fibre typing. I've been delving deep into the recesses of the brain for an answer to this, fibre typing on embalmed cadaver muscle. I'll have to use paraffin embedded muscle as cryostat sections are extremely difficult to obtain from embalmed material, at least here in Glasgow. Any and all suggestions appreciated. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Wed Jul 23 18:36:23 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Jul 23 18:36:30 2008 Subject: [Histonet] Friday In-Reply-To: <009101c8ed10$50e73b10$0302a8c0@yourxhtr8hvc4p> Message-ID: Hey, What Who woke me up? Still early in the morning here in Australia. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, 24 July 2008 8:06 AM To: Blazek, Linda; histonet@lists.utsouthwestern.edu Cc: 'Breeden, Sara' Subject: Re: [Histonet] Friday I don't know about Friday, but it's 5 o'clock somewhere. ----- Original Message ----- From: "Blazek, Linda" To: Cc: "'Breeden, Sara'" Sent: Wednesday, July 23, 2008 2:17 PM Subject: [Histonet] Friday Hey! Is it Friday!!!!!???? Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From napoli <@t> siscom.net Wed Jul 23 19:33:07 2008 From: napoli <@t> siscom.net (napoli@siscom.net) Date: Wed Jul 23 19:33:16 2008 Subject: [Histonet] dermatopathology Message-ID: <4887cdc3.22c.4882.621110532@siscom.net> For all you "skin jobs" out there (Bladerunner reference!) I run a dermpath lab. I am wondering if anyone is interested in sharing with me the H/E protocol they use on skin specimens? I currently use Gill 3 from Richard Allan (thermo) and acetic acid alcohol for mild decolorization. The derms I work for love the stain, but I think that others demonstrate chromatin patterns better. This stain seems "overstained" to me, and it is the protocol that my predecessor used. Any comments? Also, if anyone cuts nail sections (i.e. fingernails or toenails), the method I am currently using is producing results such as I have never experienced in my 13 years in dermpath. I have worked for a number of dermatopathology groups. Perhaps you all know this one: (PS I'm new to Histonet) Prior to processing: Treat nail fragments by immersing them in a solution of 20% NaOH for anywhere from 20 minutes for small fragments to an hour for thicker sections. This is dependent on thickness and density of keratin. For very thickened nails, perhaps 4mm think or more, it could take more time. After processing: Embed as perpendicular to the blade, face in block by rotating microtome wheel at 4 micron setting until full face is obtained. Treat surface of sections with 10% KOH for anywhere from 20 minutes to one hour or more, just until keration is soft, but before completely dissolving nail. Use positively-charged slides. I use this regularly and have never been happier with the results. Perhaps you all know a better way! I would love to speed this method up, particularly in regard to the grossing part, prior to processing. From jstaruk <@t> masshistology.com Wed Jul 23 19:43:20 2008 From: jstaruk <@t> masshistology.com (jstaruk) Date: Wed Jul 23 19:43:19 2008 Subject: [Histonet] Processing and staining sawdust In-Reply-To: <4887cdc3.22c.4882.621110532@siscom.net> Message-ID: <03A8E3A7F83F49EDB9BCC464C6911826@JimPC> Hi all, I searched the archives and found nothing on this topic. I have sawdust from several trees. My mission is to process, stain and examine these specimens and try to identify the types of wood each came from. Has anyone ever performed histology on sawdust? Can I simply embed the chips in paraffin and section them or should they be routinely processed? Is the traditional H&E the correct stain to use to differentiate the woods? Any comments and suggestions are welcomed unless you plan on invoicing me! Thanks Jim _____________________ Jim Staruk Mass Histology Service www.masshistology.com From lpwenk <@t> sbcglobal.net Wed Jul 23 20:05:54 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed Jul 23 20:06:06 2008 Subject: [Histonet] Filter paper In-Reply-To: Message-ID: <002801c8ed29$6cf75650$0202a8c0@HPPav2> If memory serves me correctly, the lower the number, the thinner the paper. And for most histology stains, thin works just fine, and is faster, too. But I'm curious - what staining procedure do you do that requires celestin blue? We don't like making it up, so have either substituted an aluminum hematoxylin (like Mayer or Gill), or an iron hematoxylin (like Weigert), depending upon the stain or the type of nuclear contrast we want. And we always have aluminum and iron hematoxylins around for lots of other stains, so that saves us time from having to make up a third nuclear stain. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Myl?ne de Champlain Sent: Wednesday, July 23, 2008 6:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Filter paper Hi, To filter blue celestin, is what I have to use a filter paper number 1 or 4? Thank! Myl?ne de Champlain _________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jerry <@t> ralambusa.com Wed Jul 23 21:29:14 2008 From: Jerry <@t> ralambusa.com (Jerry Helisek) Date: Wed Jul 23 21:29:18 2008 Subject: [Histonet] RE: Whole Mount Block Storage Message-ID: <3855F92002259948A66A8CA2D16E3A4F0B5809@server.ralambusa.com> Jennifer, RA Lamb has both Metal and cardboard storage solutions for whole mount block storage (as well as larger slide storage, coverslips and cassettes). Have a great day! ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ ------------------------------ Message: 4 Date: Wed, 23 Jul 2008 10:47:09 -0700 From: "Bull, Jennifer L." Subject: [Histonet] Whole Mount Block Storage To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <85760CECEC18444BB95F26D5E88DAEAA21C1C1F3BF@hinet2.hinet.org> Content-Type: text/plain; charset="us-ascii" A few months ago, we began cutting Whole Mount blocks. I am using the Super Cassettes from Surgipath. I have been able to find all the supplies for whole mounts EXCEPT for a means to store the whole mount blocks. They do not fit in the normal block storage boxes. I would appreciate any feedback or leads from those of you that are already storing these monster blocks. Thanks! Jenny Bull Histology Supervisor Northwest Pathology jennifer.bull@northwestpathology.com ------------------------------ From AWeiss <@t> shorememorial.org Thu Jul 24 07:42:58 2008 From: AWeiss <@t> shorememorial.org (AWeiss@shorememorial.org) Date: Thu Jul 24 07:43:33 2008 Subject: [Histonet] Empty Vacuum Collection Bottles Message-ID: Does anyone use 1000.0cc vacuum bottles for collection of Effusions? If so, could you please share your supplier with me for the company I was receiving them from is having a problem for they were contaminated and there is a back order. Please let me know if anyone else has experienced this. Andrea J Weiss BST CT (ASCP) Cytotechnologist 609 653 3577 Ext 4907 aweiss@shorememorial.org This transmittal from Shore Memorial Health System is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review or use, including disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender and destroy all copies of the transmittal. From Jeannette.Mitchell <@t> vtmednet.org Thu Jul 24 07:44:01 2008 From: Jeannette.Mitchell <@t> vtmednet.org (Mitchell, Jeannette M.) Date: Thu Jul 24 07:44:07 2008 Subject: [Histonet] Fite Stain Message-ID: Quick question for everyone. We received a cytology slide in xylene( left overnight) for Fite stain. Is this ruined because of the extended time in xylene? I looked in the Carson book and here is what is written M leprae and Nocardia spp. are weakly acid fast and not alcohol fast (Chandler) so alcohol must be avoided. So we don't want to receive it in Alcohol Why a short exposure to xylene for removal of paraffin has such an adverse effect on the leprosy organism while the prolonged exposure to xylene during processing does not have the same effect to xylene during processing does not have the same effect is an interesting consideration (Stevens). According to Stevens, once the leprosy organism is adequately stained, it will resist decolonization as tenaciously as the tubercle bacillus does; instead the problem may be one of resistance to uptake of the stain rather than retention. We are having quite the discussion what is right. So I need more opinions from out there Thanks, Jeannette Mitchell, BS, HTL(ASCP), QIHC R&D Histotechnologist EP1-119 Fletcher Allen Health Care Burlington, VT 05401 Phone # 802-847-3096 & 802-847-5362 From b-frederick <@t> northwestern.edu Thu Jul 24 07:53:55 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Jul 24 07:54:05 2008 Subject: [Histonet] Please help settle debate!! In-Reply-To: <244A31EE-7FDB-465F-A779-F98CB4260464@mimectl> Message-ID: <001301c8ed8c$54daf820$d00f7ca5@lurie.northwestern.edu> Guinness for me!!!! Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Wednesday, July 23, 2008 5:07 PM Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Please help settle debate!! Only if there is only one section on a slide... But they'd still count the number of sections. Sorry, clinic politics are REALLY stressing me out today. I guess I will have to visit my buddy Jim Beam after work today. Claire And of course whoever is asking this question will be expecting exactly 1000 3 micron slides to be produced from this 3mm thick piece of tissue. Ross M Stapf Histopathology Manager Baylor University Medical Center 3500 Gaston Ave. Dallas, TX 75246 214-820-2465 214-820-4110 fax RossS@baylorhealth.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Jul 24 08:04:52 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jul 24 08:04:56 2008 Subject: [Histonet] Fite Stain In-Reply-To: Message-ID: <513022.26750.qm@web65706.mail.ac4.yahoo.com> Yes, it is probably ruined. The "wax" cover of M.leprae is much more delicate than the one in M. tuberculosis and that is why dewaxing should be done with a mixture of xylene and some oil (I always used peanut oil, until I changes to mineral oil). They are probably ruined. Ren? J. --- On Thu, 7/24/08, Mitchell, Jeannette M. wrote: Jeannette Mitchell, BS, HTL(ASCP), QIHC R&D Histotechnologist EP1-119 Fletcher Allen Health Care Burlington, VT 05401 Phone # 802-847-3096 & 802-847-5362 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lksell <@t> aol.com Thu Jul 24 08:58:05 2008 From: lksell <@t> aol.com (lksell@aol.com) Date: Thu Jul 24 08:58:48 2008 Subject: [Histonet] Anyone need a roommate? Message-ID: <8CABBAC6DEDF0A7-BD4-18CF@webmail-me02.sysops.aol.com> Hello All, My co-worker will be attending the NSH Meeting in Pittsburgh and is looking to possibly share a hotel room with. Is there anyone out there who would be interested? Leslie Sell Presbyterian Hospital Charlotte, NC 28204 704-384-5490 From jdhisto <@t> yahoo.com Thu Jul 24 10:56:18 2008 From: jdhisto <@t> yahoo.com (JD) Date: Thu Jul 24 10:56:29 2008 Subject: [Histonet] Urine sample Message-ID: Hello, How long is urine good for FISH studies? From nbroadbe <@t> ucsd.edu Thu Jul 24 11:36:56 2008 From: nbroadbe <@t> ucsd.edu (Nicola J Broadbent) Date: Thu Jul 24 11:37:02 2008 Subject: [Histonet] a silly question Message-ID: <001701c8edab$7d2079d0$8a4cef84@mszippy> Hi, I'm embarrassed to ask this, but I have a basic question about making percent solutions (% w/v). I wish to make a 2.5% solution of drug A in saline. I have 10mg of drug A and want to know how much saline to add to get a 2.5% solution. The formula I found for making % solutions is: %solution = (dry mass in grams/volume in mls) *100 According to this formula, I would need to add 0.4ml to 10mg to get a 2.5% solution. I am not sure whether this is correct however, as my intuition is to keep the units the same (mg/mls) and here the amount of saline to be added would be 400ml. I know this is a bit stupid and I can only claim that it is close to Friday and my brain is shutting down...Can someone resolve this for me? Help! Nicola J. Broadbent From ttruscot <@t> vetmed.wsu.edu Thu Jul 24 12:02:17 2008 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Thu Jul 24 12:02:24 2008 Subject: [Histonet] a silly question In-Reply-To: <001701c8edab$7d2079d0$8a4cef84@mszippy> References: <001701c8edab$7d2079d0$8a4cef84@mszippy> Message-ID: <35CF12B690D8CA4E95375A36B4E7B44CB6BEDE@cvm36.vetmed.wsu.edu> Hi Nicola, Ask yourself, Would adding 10mg to .4ml be the same as adding 2.5 gm to 100 ml? or would adding 10mg. to 400ml be the same as adding 2.5 gm to 100ml? Good luck< Tom T. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicola J Broadbent Sent: Thursday, July 24, 2008 8:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] a silly question Hi, I'm embarrassed to ask this, but I have a basic question about making percent solutions (% w/v). I wish to make a 2.5% solution of drug A in saline. I have 10mg of drug A and want to know how much saline to add to get a 2.5% solution. The formula I found for making % solutions is: %solution = (dry mass in grams/volume in mls) *100 According to this formula, I would need to add 0.4ml to 10mg to get a 2.5% solution. I am not sure whether this is correct however, as my intuition is to keep the units the same (mg/mls) and here the amount of saline to be added would be 400ml. I know this is a bit stupid and I can only claim that it is close to Friday and my brain is shutting down...Can someone resolve this for me? Help! Nicola J. Broadbent _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From renafail <@t> bellsouth.net Thu Jul 24 12:02:55 2008 From: renafail <@t> bellsouth.net (Rena Fail) Date: Thu Jul 24 12:04:19 2008 Subject: [Histonet] Fite Stain In-Reply-To: Message-ID: <000801c8edaf$1ea6d990$0301a8c0@RENAD4YK9B8ABE> No, it is not ruined. Even if this slide had been brought to you in alcohol, you could still stain it5 with the Fite's method by removing the alcohol and starting over in the xylene/peanut oil. If you want to this out take 4 slides of a M. leprae put one in xylene overnight, deparaffinize one in xylene and leave in alcohol overnight, and stain one correctly BUT dehydrate in alcohols instead of air drying. The next day take those 3 slides and a fresh slide and start the stain over. Rena Fail -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Jeannette M. Sent: Thursday, July 24, 2008 8:44 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Fite Stain Quick question for everyone. We received a cytology slide in xylene( left overnight) for Fite stain. Is this ruined because of the extended time in xylene? I looked in the Carson book and here is what is written M leprae and Nocardia spp. are weakly acid fast and not alcohol fast (Chandler) so alcohol must be avoided. So we don't want to receive it in Alcohol Why a short exposure to xylene for removal of paraffin has such an adverse effect on the leprosy organism while the prolonged exposure to xylene during processing does not have the same effect to xylene during processing does not have the same effect is an interesting consideration (Stevens). According to Stevens, once the leprosy organism is adequately stained, it will resist decolonization as tenaciously as the tubercle bacillus does; instead the problem may be one of resistance to uptake of the stain rather than retention. We are having quite the discussion what is right. So I need more opinions from out there Thanks, Jeannette Mitchell, BS, HTL(ASCP), QIHC R&D Histotechnologist EP1-119 Fletcher Allen Health Care Burlington, VT 05401 Phone # 802-847-3096 & 802-847-5362 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From detmar <@t> mshri.on.ca Thu Jul 24 12:17:28 2008 From: detmar <@t> mshri.on.ca (Jacqui Detmar) Date: Thu Jul 24 12:17:55 2008 Subject: [Histonet] a silly question In-Reply-To: <35CF12B690D8CA4E95375A36B4E7B44CB6BEDE@cvm36.vetmed.wsu.edu> References: <001701c8edab$7d2079d0$8a4cef84@mszippy> <35CF12B690D8CA4E95375A36B4E7B44CB6BEDE@cvm36.vetmed.wsu.edu> Message-ID: Hi Nicola. This is actually not a stupid question at all. When I first started my MSc, I had no friggin' clue how to make a solution and over the years I've had to teach myself the basics and the more advanced stuff. In addition, I've come across numerous PhDs who *still* don't know how to make solutions! The best way to consider a percent solution is to think of things in terms of 100 mL. A 10% solution (w/v) is 10 grams of your solute in a total volume of 100 mL (this final volume being the volume of your solute + solvent). Anyway, it's a good thing you checked b/c adding 400 mL of saline would be too much. The way to make such a solution is to add your powder (10 mg) to a microfuge tube and *raise* the level of the solution to 400 uL (microlitres) using saline, to get a 2.5% (w/v) solution; however, this is rather difficult using such small volumes (and microfuge tube gradations are not that accurate), so you can totally get away with simply adding 400 uL of saline to your 10 mg of drug in the tube. ----------------------- I got this volume by cross-multiplication: 0.01 g (drug)/X (volume in mL) = 2.5 g/100 mL (to give you 2.5% solution) Solve for x: X = (0.01 g x 100 mL)/2.5 g (cross out units and you're left with mL) = 0.4 mL = 400 uL ------------------------ Note that b/c your concentration is relatively small (2.5%) you can get away with measuring your powder out and adding the final volume; however, you cannot do this with high-concentration (eg. 25-80% w/v) solutions. What you need to do in this case is measure your powder, add it to the flask and raise the solution to the final volume you want. If you don't do this and you simply add the volume to your powder, your final volume will be *much* higher than you want. This translates into a more dilute solution. I hope I'm making myself clear? It's rather difficult explaining this via email instead of drawing pictures and doing the calculations in front of the students, like I normally do. Anyway, if you need more info, please feel free to let me know. Jacqui Jacqui Detmar, Post-doctoral Fellow Samuel Lunenfeld Research Institute, Mount Sinai Hospital 25 Orde Street, room 6-1001 AJ, Toronto, ON, Canada M5T 3H7 phone: 416-586-4800 x5607 fax: 416-586-8588 email: detmar@mshri.on.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Truscott, Tom Sent: Thursday, July 24, 2008 1:02 PM To: nbroadbent@ucsd.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] a silly question Hi Nicola, Ask yourself, Would adding 10mg to .4ml be the same as adding 2.5 gm to 100 ml? or would adding 10mg. to 400ml be the same as adding 2.5 gm to 100ml? Good luck< Tom T. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nicola J Broadbent Sent: Thursday, July 24, 2008 8:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] a silly question Hi, I'm embarrassed to ask this, but I have a basic question about making percent solutions (% w/v). I wish to make a 2.5% solution of drug A in saline. I have 10mg of drug A and want to know how much saline to add to get a 2.5% solution. The formula I found for making % solutions is: %solution = (dry mass in grams/volume in mls) *100 According to this formula, I would need to add 0.4ml to 10mg to get a 2.5% solution. I am not sure whether this is correct however, as my intuition is to keep the units the same (mg/mls) and here the amount of saline to be added would be 400ml. I know this is a bit stupid and I can only claim that it is close to Friday and my brain is shutting down...Can someone resolve this for me? Help! Nicola J. Broadbent _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jengirl1014 <@t> yahoo.com Thu Jul 24 12:25:58 2008 From: jengirl1014 <@t> yahoo.com (Jennifer Sipes) Date: Thu Jul 24 12:26:01 2008 Subject: [Histonet] anyone doing kidneys in general? Message-ID: <638650.34282.qm@web62414.mail.re1.yahoo.com> Hi everyone! We're looking at the proximal tubules in mice and we're finding that they are either collapsed or whole looking but the edges are crinkled.? I'm perfusing the kidney, but I'm wondering if anyone had any suggestions on how to get nice whole looking proximal tubules?? Should I maybe perfuse more to ensure that they're opening up for us or is there another method? Please let me know and thanks in advance for all your help! Jennifer K. Sipes, ALAT Sr. Laboratory Technician Johns Hopkins University Ross 929 and Ross 933 720 Rutland Avenue Baltimore, MD? 21205 phone:? 410-614-0131 ?????????????? 410-955-9688 fax:???????? 410-955-9677 cell:??????? 443-631-6361 e-mail:? jsipes1@jhmi.edu From nbroadbe <@t> ucsd.edu Thu Jul 24 12:28:26 2008 From: nbroadbe <@t> ucsd.edu (Nicola J Broadbent) Date: Thu Jul 24 12:28:29 2008 Subject: [Histonet] thanks Message-ID: <003101c8edb2$aeb94c40$8a4cef84@mszippy> Thank you to everyone that replied to my question regarding %solutions (w/v). I've got it now! And I will be explaining the answer to my boss (yes, a PhD!!!...just like me, sigh!) :-) All the best everyone, thanks Nicola J. Broadbent From slappycraw <@t> yahoo.com Thu Jul 24 12:39:21 2008 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Thu Jul 24 12:39:26 2008 Subject: [Histonet] F4/80 alk Phos on mouse tissue Message-ID: <981164.66675.qm@web53611.mail.re2.yahoo.com> Anyone out there in histoland using F4/80 on mouse tissue with alk phos? Larry A. Woody Seattle, Wa. From carrolpb <@t> umdnj.edu Thu Jul 24 12:42:09 2008 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Thu Jul 24 12:42:41 2008 Subject: [Histonet] a silly question In-Reply-To: <001701c8edab$7d2079d0$8a4cef84@mszippy> References: <001701c8edab$7d2079d0$8a4cef84@mszippy> Message-ID: <4888BEF1.3080406@umdnj.edu> > I have 10mg of drug A and want to know how much saline to add to get a 2.5% solution I'd help you on this one, but I'm still busy racking my brain trying to figure out how many microns are in 3mm :-P From liz <@t> premierlab.com Thu Jul 24 12:48:36 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Jul 24 12:48:47 2008 Subject: [Histonet] F4/80 alk Phos on mouse tissue In-Reply-To: <981164.66675.qm@web53611.mail.re2.yahoo.com> References: <981164.66675.qm@web53611.mail.re2.yahoo.com> Message-ID: Larry We have protocols in place for both DAB and Alk phos - fast red for F4/80. What do you need. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Larry Woody Sent: Thursday, July 24, 2008 11:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] F4/80 alk Phos on mouse tissue Anyone out there in histoland using F4/80 on mouse tissue with alk phos? Larry A. Woody Seattle, Wa. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Thu Jul 24 12:53:34 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu Jul 24 13:02:59 2008 Subject: [Histonet] a silly question In-Reply-To: <4888BEF1.3080406@umdnj.edu> References: <001701c8edab$7d2079d0$8a4cef84@mszippy> <4888BEF1.3080406@umdnj.edu> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A7F23D8B@LTA3VS011.ees.hhs.gov> And I want to know what kind of drug it is. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Peter Carroll Sent: Thursday, July 24, 2008 1:42 PM Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] a silly question > I have 10mg of drug A and want to know how much saline to add to get a 2.5% solution I'd help you on this one, but I'm still busy racking my brain trying to figure out how many microns are in 3mm :-P _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Thu Jul 24 13:20:43 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Jul 24 13:21:08 2008 Subject: [Histonet] a silly question In-Reply-To: <1CE1847DFEA0A647B1CCDE4108EA60A7F23D8B@LTA3VS011.ees.hhs.gov> Message-ID: I don't think she has enough to share . . . . "Bartlett, Jeanine (CDC/CCID/NCZVED)" Sent by: histonet-bounces@lists.utsouthwestern.edu 07/24/2008 12:53 PM To "Peter Carroll" cc histonet@lists.utsouthwestern.edu Subject RE: [Histonet] a silly question And I want to know what kind of drug it is. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Peter Carroll Sent: Thursday, July 24, 2008 1:42 PM Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] a silly question > I have 10mg of drug A and want to know how much saline to add to get a 2.5% solution I'd help you on this one, but I'm still busy racking my brain trying to figure out how many microns are in 3mm :-P _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vanikodela <@t> hotmail.com Thu Jul 24 13:24:49 2008 From: vanikodela <@t> hotmail.com (vani) Date: Thu Jul 24 13:24:56 2008 Subject: [Histonet] CBG Biotech Recycler for sale Message-ID: We have a 2 1/2-year old CBG Biotech solvent recycler, 5 gallon model, that we no longer need and would like to sell. We hardly used it and it is in excellent condition. Moreover, it has an extended warranty backed by CBG Biotech until December 2011. We are a research lab and used it to recycle alcohol and xylene, but our volume of use was much lower than a clinical histology lab. Therefore, the unit is relatively unused and almost like new, with the manual and accessories (carboys). A photo of the unit is posted on http://www.histonet.org, under the file name "CBG Biotech Recycler2.jpg" We are selling it because the project for which we purchased it is finished. Initially, we thought it would be needed for a longer time. As for price, we are seeking to sell it for half the purchase price, around $7000 (including the price of the extended warranty), or best offer. Please be reminded that proper functioning of the unit is guaranteed under the extended warranty, until December 2011. Please contact Vani at kodela@md-partners.com From JWeems <@t> sjha.org Thu Jul 24 13:26:46 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Jul 24 13:26:49 2008 Subject: [Histonet] Urine sample In-Reply-To: References: Message-ID: <982A0A9461F9BF438C7B19A6E425A383434E08@ITSSSXM01V6.one.ads.che.org> For Urovysion? - You need to add Carbowax to it within 6 hrs and ship within 24 hrs if you're sending it out. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of JD Sent: Thursday, July 24, 2008 11:56 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Urine sample Hello, How long is urine good for FISH studies?_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From Jackie.O'Connor <@t> abbott.com Thu Jul 24 13:27:44 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Jul 24 13:28:04 2008 Subject: [Histonet] Urine sample In-Reply-To: <60bv13$99njqu@viper.abbott.com> Message-ID: Are you talking about those fish in the Amazon that will follow a trail of urine? Yeah - almost Friday . . . I'm on a roll. . . . . . JD Sent by: histonet-bounces@lists.utsouthwestern.edu 07/24/2008 10:56 AM To cc Subject [Histonet] Urine sample Hello, How long is urine good for FISH studies?_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From denise.woodward <@t> uconn.edu Thu Jul 24 13:44:11 2008 From: denise.woodward <@t> uconn.edu (Woodward, Denise) Date: Thu Jul 24 13:44:18 2008 Subject: [Histonet] Processing and staining sawdust In-Reply-To: <03A8E3A7F83F49EDB9BCC464C6911826@JimPC> References: <4887cdc3.22c.4882.621110532@siscom.net> <03A8E3A7F83F49EDB9BCC464C6911826@JimPC> Message-ID: <40AC6D73C2B95C4CA21B26B7BF380C4002D0E3F3@EXCHANGED.mgmt.ad.uconn.edu> Hi Jim, I believe someone at the Yale School of Forestry was doing histology on wood. You might want to try giving them a call. Searching at www.yale.edu will get you there I believe. Good luck, Denise Long Woodward -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jstaruk Sent: Wednesday, July 23, 2008 8:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processing and staining sawdust Hi all, I searched the archives and found nothing on this topic. I have sawdust from several trees. My mission is to process, stain and examine these specimens and try to identify the types of wood each came from. Has anyone ever performed histology on sawdust? Can I simply embed the chips in paraffin and section them or should they be routinely processed? Is the traditional H&E the correct stain to use to differentiate the woods? Any comments and suggestions are welcomed unless you plan on invoicing me! Thanks Jim _____________________ Jim Staruk Mass Histology Service www.masshistology.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtitford <@t> aol.com Thu Jul 24 13:46:08 2008 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Thu Jul 24 13:46:28 2008 Subject: [Histonet] Fibre typing cadaver muscle Message-ID: <8CABBD4AB47ADEB-BF4-7FC@FWM-D26.sysops.aol.com> Dr Montgomery asked about fibre typing embalmed cadaver muscle; There was an article published in Modern Pathology in 1998 entitled, " Neuropathologic applications of immunohistochemical fiber typing in the non-neoplastic muscle biopsy" ( Modern Pathology 11: 334 -338)?where they claimed you can fibre type using MY-32 antibody on paraffin sections. This antibody reacts with fast twitch skeletal myosin.They show photographs that look pretty good...... I don't know if it would work on cadaver muscle, but if it is important important enough, it might be worth a try. Michael Titford Pathology USA Mobile AL From jdhisto <@t> yahoo.com Thu Jul 24 13:46:32 2008 From: jdhisto <@t> yahoo.com (JD) Date: Thu Jul 24 13:46:42 2008 Subject: [Histonet] (no subject) Message-ID: Thanks for the reply. And yes to be sent to "urovysion". And to the whole amazon fish / urine comment; enjoy your weekend that has apparently started early, I need a little of what you have. Ha ha I appreciate the comments. Have a great weekend. From rjbuesa <@t> yahoo.com Thu Jul 24 14:11:31 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jul 24 14:11:35 2008 Subject: [Histonet] a silly question In-Reply-To: <001701c8edab$7d2079d0$8a4cef84@mszippy> Message-ID: <895039.14054.qm@web65710.mail.ac4.yahoo.com> You calculation is correct: dissolve your 10 mg in 0.4 mL and you will get the 2.5% solution. Ren? J --- On Thu, 7/24/08, Nicola J Broadbent wrote: From: Nicola J Broadbent Subject: [Histonet] a silly question To: histonet@lists.utsouthwestern.edu Date: Thursday, July 24, 2008, 12:36 PM Hi, I'm embarrassed to ask this, but I have a basic question about making percent solutions (% w/v). I wish to make a 2.5% solution of drug A in saline. I have 10mg of drug A and want to know how much saline to add to get a 2.5% solution. The formula I found for making % solutions is: %solution = (dry mass in grams/volume in mls) *100 According to this formula, I would need to add 0.4ml to 10mg to get a 2.5% solution. I am not sure whether this is correct however, as my intuition is to keep the units the same (mg/mls) and here the amount of saline to be added would be 400ml. I know this is a bit stupid and I can only claim that it is close to Friday and my brain is shutting down...Can someone resolve this for me? Help! Nicola J. Broadbent _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Thu Jul 24 15:22:20 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Thu Jul 24 15:22:24 2008 Subject: [Histonet] Filter paper References: Message-ID: <002e01c8edca$f9ade900$6501a8c0@Sunney> What is important is not the number so much as if the paper is high retention and what is filtering speed. Whatman #4, if I remember correctly, is a medium fast paper, and should be ideal for filtering staining solution. Somewhere in the passage of time, I was told not to use paper that was really slow filtering speed and high retention for filtering dye solutions as the paper may retain too much of the dye needed. Now that could be an old wife's tale, but to this day I always use a medium fast paper. I also purchase something other than Whatman, as companies sell equivalents for a much cheaper price. Whatman 54 should also work. Big companies like ThermoScientific give the equivalents for their brand as compared to Whatman papers and are certainly more economical. Gayle M. Callis HTL/HT/MT(ASCP) ----- Original Message ----- From: "Myl?ne de Champlain" To: Sent: Wednesday, July 23, 2008 4:31 PM Subject: [Histonet] Filter paper Hi, To filter blue celestin, is what I have to use a filter paper number 1 or 4? Thank! Myl?ne de Champlain _________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgalnow <@t> aol.com Thu Jul 24 16:16:53 2008 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Thu Jul 24 16:19:49 2008 Subject: [Histonet] Filter paper Message-ID: <1178379291-1216934383-cardhu_decombobulator_blackberry.rim.net-866034243-@bxe140.bisx.prod.on.blackberry> I sometimes use coffee filters. Roxanne ------Original Message------ From: Gayle Callis Sender: histonet-bounces@lists.utsouthwestern.edu To: Myl?ne de Champlain To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Filter paper Sent: Jul 24, 2008 4:22 PM What is important is not the number so much as if the paper is high retention and what is filtering speed. Whatman #4, if I remember correctly, is a medium fast paper, and should be ideal for filtering staining solution. Somewhere in the passage of time, I was told not to use paper that was really slow filtering speed and high retention for filtering dye solutions as the paper may retain too much of the dye needed. Now that could be an old wife's tale, but to this day I always use a medium fast paper. I also purchase something other than Whatman, as companies sell equivalents for a much cheaper price. Whatman 54 should also work. Big companies like ThermoScientific give the equivalents for their brand as compared to Whatman papers and are certainly more economical. Gayle M. Callis HTL/HT/MT(ASCP) ----- Original Message ----- From: "Myl?ne de Champlain" To: Sent: Wednesday, July 23, 2008 4:31 PM Subject: [Histonet] Filter paper Hi, To filter blue celestin, is what I have to use a filter paper number 1 or 4? Thank! Myl?ne de Champlain _________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sent via BlackBerry by AT&T From JWeems <@t> sjha.org Thu Jul 24 17:45:10 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Jul 24 17:45:14 2008 Subject: [Histonet] Filter paper In-Reply-To: <1178379291-1216934383-cardhu_decombobulator_blackberry.rim.net-866034243-@bxe140.bisx.prod.on.blackberry> References: <1178379291-1216934383-cardhu_decombobulator_blackberry.rim.net-866034243-@bxe140.bisx.prod.on.blackberry> Message-ID: <982A0A9461F9BF438C7B19A6E425A383434F02@ITSSSXM01V6.one.ads.che.org> And paper towels will work when you have run out of filter paper... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Thursday, July 24, 2008 5:17 PM To: Gayle Callis; histonet-bounces@lists.utsouthwestern.edu; Myl?ne de Champlain; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Filter paper I sometimes use coffee filters. Roxanne ------Original Message------ From: Gayle Callis Sender: histonet-bounces@lists.utsouthwestern.edu To: Myl?ne de Champlain To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Filter paper Sent: Jul 24, 2008 4:22 PM What is important is not the number so much as if the paper is high retention and what is filtering speed. Whatman #4, if I remember correctly, is a medium fast paper, and should be ideal for filtering staining solution. Somewhere in the passage of time, I was told not to use paper that was really slow filtering speed and high retention for filtering dye solutions as the paper may retain too much of the dye needed. Now that could be an old wife's tale, but to this day I always use a medium fast paper. I also purchase something other than Whatman, as companies sell equivalents for a much cheaper price. Whatman 54 should also work. Big companies like ThermoScientific give the equivalents for their brand as compared to Whatman papers and are certainly more economical. Gayle M. Callis HTL/HT/MT(ASCP) ----- Original Message ----- From: "Myl?ne de Champlain" To: Sent: Wednesday, July 23, 2008 4:31 PM Subject: [Histonet] Filter paper Hi, To filter blue celestin, is what I have to use a filter paper number 1 or 4? Thank! Myl?ne de Champlain _________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sent via BlackBerry by AT&T Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From tjasper <@t> copc.net Thu Jul 24 18:49:56 2008 From: tjasper <@t> copc.net (Thomas Jasper) Date: Thu Jul 24 18:50:01 2008 Subject: [Histonet] PAX-2 Message-ID: <90354A475B420441B2A0396E5008D4965E2114@copc-sbs.COPC.local> Hey there folks, Anyone running PAX-2? I've had no luck finding it as a pre-dilute and have only found one vendor with a concentrate. So...anyone know of a pre-dilute out there and are you running it? Or if you're running it as a concentrate, what is the titration? Human clinical laboratory application is what I'm interested in. Thanks in advance for any assistance here. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, OR 97701 541/693-2677 tjasper@copc.net From christina.thurby <@t> bms.com Fri Jul 25 07:48:03 2008 From: christina.thurby <@t> bms.com (Christina Thurby) Date: Fri Jul 25 07:49:34 2008 Subject: [Histonet] Re: Canine brain tissue for IHC control In-Reply-To: <200807241705.m6OH5AWG014844@meusplapp01.net.bms.com> References: <200807241705.m6OH5AWG014844@meusplapp01.net.bms.com> Message-ID: <4889CB83.4010009@bms.com> Hello, Does anyone out there know where I can purchase canine brain tissue control blocks for IHC? I am looking for a block from an animal that is aged (>12 yrs old) if possible. Thanks, Christina Thurby Bristol Myers Squibb 812-429-8097 From Janci.R.Wellborn <@t> questdiagnostics.com Fri Jul 25 09:37:12 2008 From: Janci.R.Wellborn <@t> questdiagnostics.com (Wellborn, Janci R) Date: Fri Jul 25 09:37:41 2008 Subject: [Histonet] Filter paper In-Reply-To: <982A0A9461F9BF438C7B19A6E425A383434F02@ITSSSXM01V6.one.ads.che.org> References: <1178379291-1216934383-cardhu_decombobulator_blackberry.rim.net-866034243-@bxe140.bisx.prod.on.blackberry> <982A0A9461F9BF438C7B19A6E425A383434F02@ITSSSXM01V6.one.ads.che.org> Message-ID: <37B884B975303247A3C707ACB9C1343904C71C08@QDCWS0278.us.qdx.com> Both help to keep costs down . . . -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Thursday, July 24, 2008 5:45 PM To: godsgalnow@aol.com; Gayle Callis; histonet-bounces@lists.utsouthwestern.edu; Myl?ne de Champlain; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Filter paper And paper towels will work when you have run out of filter paper... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Thursday, July 24, 2008 5:17 PM To: Gayle Callis; histonet-bounces@lists.utsouthwestern.edu; Myl?ne de Champlain; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Filter paper I sometimes use coffee filters. Roxanne ------Original Message------ From: Gayle Callis Sender: histonet-bounces@lists.utsouthwestern.edu To: Myl?ne de Champlain To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Filter paper Sent: Jul 24, 2008 4:22 PM What is important is not the number so much as if the paper is high retention and what is filtering speed. Whatman #4, if I remember correctly, is a medium fast paper, and should be ideal for filtering staining solution. Somewhere in the passage of time, I was told not to use paper that was really slow filtering speed and high retention for filtering dye solutions as the paper may retain too much of the dye needed. Now that could be an old wife's tale, but to this day I always use a medium fast paper. I also purchase something other than Whatman, as companies sell equivalents for a much cheaper price. Whatman 54 should also work. Big companies like ThermoScientific give the equivalents for their brand as compared to Whatman papers and are certainly more economical. Gayle M. Callis HTL/HT/MT(ASCP) ----- Original Message ----- From: "Myl?ne de Champlain" To: Sent: Wednesday, July 23, 2008 4:31 PM Subject: [Histonet] Filter paper Hi, To filter blue celestin, is what I have to use a filter paper number 1 or 4? Thank! Myl?ne de Champlain _________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sent via BlackBerry by AT&T Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------ The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. From JimR0712 <@t> comcast.net Fri Jul 25 10:27:59 2008 From: JimR0712 <@t> comcast.net (JimR0712@comcast.net) Date: Fri Jul 25 10:28:07 2008 Subject: [Histonet] Voice recognition systems and Copath Plus from Misys Message-ID: <072520081527.22288.4889F0FF000B15B5000057102216527966CDCEC9CFAD0307B6@comcast.net> Would like to get feedback from Misys Copath Plus users who have voice recognition. System used. Likes and dislikes. Thanks. From ploykasek <@t> phenopath.com Fri Jul 25 11:13:52 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri Jul 25 11:14:02 2008 Subject: [Histonet] PAX-2 In-Reply-To: <90354A475B420441B2A0396E5008D4965E2114@copc-sbs.COPC.local> Message-ID: Hi Thomas. Zymed has a Pax-2 concentrate, I don't know if they have a pre-dilute available. We use it on paraffin embedded human tissue with a heat citrate pretreatment. I always think it best to determine your own optimal titer - so variable depending on fixation, processing, pretreatment, detection, etc.... We always start with the manufacturers recommendation & do a 'sandwich titer'. For example, if recommended is 1:100, we do 1:50, 1:100, & 1:250. Hope this helps. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Hey there folks, > > Anyone running PAX-2? I've had no luck finding it as a pre-dilute and > have only found one vendor with a concentrate. So...anyone know of a > pre-dilute out there and are you running it? Or if you're running it as > a concentrate, what is the titration? Human clinical laboratory > application is what I'm interested in. Thanks in advance for any > assistance here. > > Thomas Jasper HT (ASCP) BAS > Histology Supervisor > Central Oregon Regional Pathology Services > Bend, OR 97701 > 541/693-2677 > tjasper@copc.net > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From CBark <@t> memorialcare.org Fri Jul 25 12:52:34 2008 From: CBark <@t> memorialcare.org (Christine Bark) Date: Fri Jul 25 12:52:54 2008 Subject: [Histonet] Sakura VIP alarm Message-ID: Happy Friday Histonetters!! I need to find an alarm system for my Sakura VIP E and VIP 5. Our current alarm system will become obsolete very soon as we are moving (too far apparently for main lab to hear). I've heard of a system that can page/phone someone when the alarm goes off but I can't seem to find out any more information. What is everyone else doing about alarms going off after-hours? Any and every response is welcome, including vendors. Thank you, Christine Bark HT(ASCP) CM Lead Histotech, Pathology Saddleback Memorial Medical Center 949-452-3548 cbark@memorialcare.org ______________________________________________________________________________ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From tjasper <@t> copc.net Fri Jul 25 13:23:41 2008 From: tjasper <@t> copc.net (Thomas Jasper) Date: Fri Jul 25 13:23:47 2008 Subject: [Histonet] Response to PAX-2 Message-ID: <90354A475B420441B2A0396E5008D4965E2118@copc-sbs.COPC.local> I'd like to thank everyone for responding so quickly about PAX-2. I've got all the information I need. We'll be moving forward with whichever option our pathologists decide upon. Thanks again. Tom J. From cmiller <@t> physlab.com Fri Jul 25 15:22:16 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Fri Jul 25 15:24:45 2008 Subject: [Histonet] CAP Inspection Message-ID: <000001c8ee94$21ed27e0$3d02a8c0@plab.local> I am almost to the finish line. CAP came knocking at 800 am this morning. My Inspector is very nice. But I am her first inspection and she has left no stone unturned. Its been a long grueling 7 hours and she ran out of time!!! She has to cut it short to get her report ready for the summation. I am so glad its Friday!!!!! now if I can survive the summation More on Monday, Cheri Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From TMSykora <@t> gundluth.org Fri Jul 25 22:00:38 2008 From: TMSykora <@t> gundluth.org (TMSykora@gundluth.org) Date: Fri Jul 25 22:01:07 2008 Subject: [Histonet] Theresa M Sykora/CytHist/LAX/GUNDLUTH is out of the office. Message-ID: I will be out of the office starting 07/25/2008 and will not return until 08/04/2008. I will respond to your message when I return. From lldewe <@t> gmail.com Sat Jul 26 12:41:33 2008 From: lldewe <@t> gmail.com (Loralei Dewe) Date: Sat Jul 26 12:41:39 2008 Subject: [Histonet] Upcoming Webinar Message-ID: <7173d3c00807261041w4a7affe6p182a040222f6dd28@mail.gmail.com> You are invited to attend a live, interactive, web-based tutorial: *Counting, Sorting and Categorizing Objects in Images* *Presenter Name: Loralie Dewe, Applications Specialist, Media Cybernetics, a MAG Company * * * *Details are below. Connection lines are limited, so reserve yours now. There is no charge to participate in this on-line seminar.* * * * * *July 29, 2008 Tuesday at 10:30 AM Pacific Time* *http://www.magworldwide.com/education/webinar07150801.php* * * *=====================* *Counting objects in images is a task that is routinely performed by scientists in a variety of disciplines. Researchers in material sciences and biology often share the goal of measuring the distribution of particles, cells, and other objects in images based on size, shape, clumpiness, roundness, smoothness, density, color, and other features. Identification and classification of objects in images that contain mixed populations into subgroups based on differences in morphological and optical properties can be tedious, time-consuming, and subject to error unless assisted by image processing tools that are designed for the task. Attendees of this free web-based seminar will learn how to achieve maximum efficiency, accuracy and reproducibility in their analysis by applying a sequence of processing steps to their images and will see how to leverage common feature-extraction and measurement tools to categorize, sort, count, and graph their data. Bring your questions to this live, interactive web-based seminar. Subjects include: * - *Enhancing images for accurate processing * - *Performing thresholding operations to separate objects-of-interest from background * - *Utilizing spatial and morphological filters to differentiate populations of objects * - *Capturing and presenting your data * - *Archiving and databasing of images * *Provided free of charge, this webinar is sponsored by MAG, the Microimaging Applications Group. MAG is a group of imaging companies who work together to provide an unparalleled range of microimaging solutions to science and industry. * * * * * *Loralei Dewe* *MicroImaging Applications Group* From shehnazster <@t> gmail.com Sun Jul 27 12:00:07 2008 From: shehnazster <@t> gmail.com (shehnaz khan) Date: Sun Jul 27 12:00:14 2008 Subject: [Histonet] RNA isolation from tissue Message-ID: <4fe9f16e0807271000oef12fd1h4c58d99eaaa6c039@mail.gmail.com> Can anyone help / offer any suggestions on extracting RNA from fresh frozen breast tissue. Following the usual cleaning, wiping down with RNAse Away / Zap etc. We're using Trizol Reagent to store / collect samples in and then immediately snap freeze. RNA is isolated using the Trizol method. The RNA is severely degraded. Please Help! S. Khan From gayle.callis <@t> bresnan.net Sun Jul 27 12:59:16 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Sun Jul 27 12:59:18 2008 Subject: [Histonet] Trouble with Histonet Message-ID: <000801c8f012$7c445630$6401a8c0@Sunney> I have tried to get into Histonet Archives for three days via Firefox,and was refused each time. Is there a problem with the website? This has never happened before. Gayle M. Callis From detmar <@t> mshri.on.ca Sun Jul 27 14:06:12 2008 From: detmar <@t> mshri.on.ca (Jacqui Detmar) Date: Sun Jul 27 14:06:31 2008 Subject: [Histonet] RNA isolation from tissue In-Reply-To: <4fe9f16e0807271000oef12fd1h4c58d99eaaa6c039@mail.gmail.com> References: <4fe9f16e0807271000oef12fd1h4c58d99eaaa6c039@mail.gmail.com> Message-ID: Hi there. I just have a couple of comments/questions about this: 1. Are you sure you are using the correct ratio of Trizol to tissue? If you have too much tissue, the Trizol won't protect against RNases. 2. Have you tested the materials you are using to extract the RNA? i.e. the isopropanol, the DEPC-water, the 75% ethanol? 3. What kind of gel are you running to determine RNA quality? Have you tested the running buffer, the DEPC-water, the loading buffer, the agarose? 4. Are you using molecular biology grade reagents? 5. Are you running another kind of sample alongside the breast tissue as a control? Breast tissue shouldn't be *that* prone to degradation, compared to tissues like placenta, pancreas. 6. Have you tried putting the tissue immediately into RNAlater, then extracting RNA? A colleague who works with mouse pancreatic tissue said this stuff saved her sanity after multiple failures with different reagents. I think that's about it. If you have any other questions, please feel free to contact me. Good luck!! Jacqui Jacqui Detmar, Post-doctoral Fellow Samuel Lunenfeld Research Institute, Mount Sinai Hospital 25 Orde Street, room 6-1001 AJ, Toronto, ON, Canada M5T 3H7 phone: 416-586-4800 x5607 fax: 416-586-8588 email: detmar@mshri.on.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of shehnaz khan Sent: Sunday, July 27, 2008 1:00 PM To: histonet@pathology.swmed.edu Subject: [Histonet] RNA isolation from tissue Can anyone help / offer any suggestions on extracting RNA from fresh frozen breast tissue. Following the usual cleaning, wiping down with RNAse Away / Zap etc. We're using Trizol Reagent to store / collect samples in and then immediately snap freeze. RNA is isolated using the Trizol method. The RNA is severely degraded. Please Help! S. Khan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From renafail <@t> bellsouth.net Sun Jul 27 14:33:25 2008 From: renafail <@t> bellsouth.net (Rena Fail) Date: Sun Jul 27 14:35:10 2008 Subject: [Histonet] Trouble with Histonet In-Reply-To: <000801c8f012$7c445630$6401a8c0@Sunney> Message-ID: <000001c8f01f$a46b5890$0301a8c0@RENAD4YK9B8ABE> Gayle, I tried explorer and firefox neither worked -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Sunday, July 27, 2008 1:59 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Trouble with Histonet I have tried to get into Histonet Archives for three days via Firefox,and was refused each time. Is there a problem with the website? This has never happened before. Gayle M. Callis _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Sun Jul 27 15:09:21 2008 From: anh2006 <@t> med.cornell.edu (anh2006@med.cornell.edu) Date: Sun Jul 27 15:12:10 2008 Subject: [Histonet] RNA isolation from tissue In-Reply-To: References: <4fe9f16e0807271000oef12fd1h4c58d99eaaa6c039@mail.gmail.com> Message-ID: <1514600784-1217189521-cardhu_decombobulator_blackberry.rim.net-2126339056-@bxe157.bisx.prod.on.blackberry> Great advice. Also are you using barrier tips? -----Original Message----- From: Jacqui Detmar Date: Sun, 27 Jul 2008 15:06:12 To: shehnaz khan; Subject: RE: [Histonet] RNA isolation from tissue Hi there. I just have a couple of comments/questions about this: 1. Are you sure you are using the correct ratio of Trizol to tissue? If you have too much tissue, the Trizol won't protect against RNases. 2. Have you tested the materials you are using to extract the RNA? i.e. the isopropanol, the DEPC-water, the 75% ethanol? 3. What kind of gel are you running to determine RNA quality? Have you tested the running buffer, the DEPC-water, the loading buffer, the agarose? 4. Are you using molecular biology grade reagents? 5. Are you running another kind of sample alongside the breast tissue as a control? Breast tissue shouldn't be *that* prone to degradation, compared to tissues like placenta, pancreas. 6. Have you tried putting the tissue immediately into RNAlater, then extracting RNA? A colleague who works with mouse pancreatic tissue said this stuff saved her sanity after multiple failures with different reagents. I think that's about it. If you have any other questions, please feel free to contact me. Good luck!! Jacqui Jacqui Detmar, Post-doctoral Fellow Samuel Lunenfeld Research Institute, Mount Sinai Hospital 25 Orde Street, room 6-1001 AJ, Toronto, ON, Canada M5T 3H7 phone: 416-586-4800 x5607 fax: 416-586-8588 email: detmar@mshri.on.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of shehnaz khan Sent: Sunday, July 27, 2008 1:00 PM To: histonet@pathology.swmed.edu Subject: [Histonet] RNA isolation from tissue Can anyone help / offer any suggestions on extracting RNA from fresh frozen breast tissue. Following the usual cleaning, wiping down with RNAse Away / Zap etc. We're using Trizol Reagent to store / collect samples in and then immediately snap freeze. RNA is isolated using the Trizol method. The RNA is severely degraded. Please Help! S. Khan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mhanna <@t> histosearch.com Sun Jul 27 15:56:33 2008 From: mhanna <@t> histosearch.com (Marvin Hanna) Date: Sun Jul 27 15:56:41 2008 Subject: [Histonet] Trouble with Histonet In-Reply-To: <000001c8f01f$a46b5890$0301a8c0@RENAD4YK9B8ABE> References: <000001c8f01f$a46b5890$0301a8c0@RENAD4YK9B8ABE> Message-ID: <2AAA3144-CA85-4F39-B4EC-D20CCD069B74@histosearch.com> Hi Gayle and Rena, It's not your browsers. I have been having problems with the server crashing the last couple of days. It's up now but I'm still working on getting the server back to stable. Sorry for the problems. Marvin Hanna On Jul 27, 2008, at 2:33 PM, Rena Fail wrote: > Gayle, I tried explorer and firefox neither worked > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle > Callis > Sent: Sunday, July 27, 2008 1:59 PM > To: histonet@pathology.swmed.edu > Subject: [Histonet] Trouble with Histonet > > I have tried to get into Histonet Archives for three days via > Firefox,and was refused each time. Is there a problem with the > website? > This has never happened before. > > Gayle M. Callis > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From relia1 <@t> earthlink.net Mon Jul 28 08:05:46 2008 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Jul 28 08:05:49 2008 Subject: [Histonet] RELIA Histology Careers Bulletin 7-29-08 Message-ID: Hi Histonetters! Supervisors, Histo Techs and New Grads; Research, Clinical, MOHS or Management. What is your next step in your career? Whatever you want to do, wherever you want to be, I can help! There is still time this summer to make that move. I have opportunities in all kinds of labs in all areas of the country. Your next position is just a phone call or an e-mail away. All of the opportunities that I represent are permanent full time positions day shift positions with companies that offer excellent salaries, benefits and relocation assistance. Here are some of the opportunities that I am most excited about: HISTOLOGY SUPERVISORS AND MANAGERS: Pathology Manager - Portland, OR Histology Supervisor - Los Angeles Histology Manager - Buffalo, NY HISTOTECHNICIANS/HISTOTECHNOLOGISTS/PA MOHS Tech for Brand New Lab - NC near the beach! Histo Tech small hospital lab new grads welcome - Western MD Histo Tech 3rd Shift Private Lab NC near the beach! Histo Tech Research environment - Seattle, WA Histo Tech Brand New Hospital Lab - Spokane, WA Histo Tech National Reference Lab - Los Angeles area Applications Support Tech for National Vendor - Chicago area PA - Private Path Lab FL lic required in Tampa Bay area Histo Tech Corporate Lab environment - Sacramento Histo Tech Private Lab - Austin, TX Grossing Tech Private Lab - Austin, TX Histo Tech with FL license hospital lab - SW FL Histo Tech with Immuno exper - Central VA If you or anyone you know are interested in hearing more about any of these opportunities or have another type of position or area in mind and would like some help in your job search please let me know. Just shoot me an e-mail at relia1@earthlink.net or give me a call toll free at 866-607-3542. Thanks-Pam Thank You! Pam M. Barker President Relia 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll Free: (866)607-3542 e-mail: relia1@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia From Janet.Bonner <@t> FLHOSP.ORG Mon Jul 28 08:29:31 2008 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Mon Jul 28 08:30:32 2008 Subject: [Histonet] Filter paper References: <1178379291-1216934383-cardhu_decombobulator_blackberry.rim.net-866034243-@bxe140.bisx.prod.on.blackberry><982A0A9461F9BF438C7B19A6E425A383434F02@ITSSSXM01V6.one.ads.che.org> <37B884B975303247A3C707ACB9C1343904C71C08@QDCWS0278.us.qdx.com> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2721@fhosxchmb006.ADVENTISTCORP.NET> Watch out that the paper you are using does not change the acidity of your solution (we had a problem with that once using the paper towels! Janet L. Bonner, HTL (ASCP) Pathology Laboratory Florida Hospital Winter Park janet.bonner@FLHOSP.org 407-646-7559 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Wellborn, Janci R Sent: Fri 7/25/2008 10:37 AM To: Weems, Joyce; godsgalnow@aol.com; Gayle Callis; histonet-bounces@lists.utsouthwestern.edu; Myl?ne de Champlain; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Filter paper Both help to keep costs down . . . -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Thursday, July 24, 2008 5:45 PM To: godsgalnow@aol.com; Gayle Callis; histonet-bounces@lists.utsouthwestern.edu; Myl?ne de Champlain; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Filter paper And paper towels will work when you have run out of filter paper... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Thursday, July 24, 2008 5:17 PM To: Gayle Callis; histonet-bounces@lists.utsouthwestern.edu; Myl?ne de Champlain; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Filter paper I sometimes use coffee filters. Roxanne ------Original Message------ From: Gayle Callis Sender: histonet-bounces@lists.utsouthwestern.edu To: Myl?ne de Champlain To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Filter paper Sent: Jul 24, 2008 4:22 PM What is important is not the number so much as if the paper is high retention and what is filtering speed. Whatman #4, if I remember correctly, is a medium fast paper, and should be ideal for filtering staining solution. Somewhere in the passage of time, I was told not to use paper that was really slow filtering speed and high retention for filtering dye solutions as the paper may retain too much of the dye needed. Now that could be an old wife's tale, but to this day I always use a medium fast paper. I also purchase something other than Whatman, as companies sell equivalents for a much cheaper price. Whatman 54 should also work. Big companies like ThermoScientific give the equivalents for their brand as compared to Whatman papers and are certainly more economical. Gayle M. Callis HTL/HT/MT(ASCP) ----- Original Message ----- From: "Myl?ne de Champlain" To: Sent: Wednesday, July 23, 2008 4:31 PM Subject: [Histonet] Filter paper Hi, To filter blue celestin, is what I have to use a filter paper number 1 or 4? Thank! Myl?ne de Champlain _________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sent via BlackBerry by AT&T Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------ The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From jdhisto <@t> yahoo.com Mon Jul 28 09:13:32 2008 From: jdhisto <@t> yahoo.com (JD) Date: Mon Jul 28 09:13:43 2008 Subject: [Histonet] Filter paper Message-ID: Nice catch. I did forget to mention to NOT use the recycled paper towels which are ran threw bleaches that can deffinately throw and/or change the accidity of a solution. Sorry about the lack of detailed information towards this matter. My apologies. Sincerely, JDhisto From gayle.callis <@t> bresnan.net Mon Jul 28 09:21:55 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Mon Jul 28 09:21:59 2008 Subject: Good advice Re: [Histonet] Filter paper References: <1178379291-1216934383-cardhu_decombobulator_blackberry.rim.net-866034243-@bxe140.bisx.prod.on.blackberry><982A0A9461F9BF438C7B19A6E425A383434F02@ITSSSXM01V6.one.ads.che.org> <37B884B975303247A3C707ACB9C1343904C71C08@QDCWS0278.us.qdx.com> <5F31F38C96781A4FBE3196EBC22D47807F2721@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: <000e01c8f0bd$4a1cdd70$6401a8c0@Sunney> RE: [Histonet] Filter paperAlso, this caveat applies to coffee filters too. Gayle M. Callis HTL,TH,MT(ASCP) ----- Original Message ----- From: Bonner, Janet To: Wellborn, Janci R ; Weems, Joyce ; godsgalnow@aol.com ; Gayle Callis ; histonet-bounces@lists.utsouthwestern.edu ; Myl?ne de Champlain ; histonet@lists.utsouthwestern.edu Sent: Monday, July 28, 2008 7:29 AM Subject: RE: [Histonet] Filter paper Watch out that the paper you are using does not change the acidity of your solution (we had a problem with that once using the paper towels! Janet L. Bonner, HTL (ASCP) Pathology Laboratory Florida Hospital Winter Park janet.bonner@FLHOSP.org 407-646-7559 ------------------------------------------------------------------------------ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Wellborn, Janci R Sent: Fri 7/25/2008 10:37 AM To: Weems, Joyce; godsgalnow@aol.com; Gayle Callis; histonet-bounces@lists.utsouthwestern.edu; Myl?ne de Champlain; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Filter paper Both help to keep costs down . . . -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Thursday, July 24, 2008 5:45 PM To: godsgalnow@aol.com; Gayle Callis; histonet-bounces@lists.utsouthwestern.edu; Myl?ne de Champlain; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Filter paper And paper towels will work when you have run out of filter paper... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of godsgalnow@aol.com Sent: Thursday, July 24, 2008 5:17 PM To: Gayle Callis; histonet-bounces@lists.utsouthwestern.edu; Myl?ne de Champlain; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Filter paper I sometimes use coffee filters. Roxanne ------Original Message------ From: Gayle Callis Sender: histonet-bounces@lists.utsouthwestern.edu To: Myl?ne de Champlain To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Filter paper Sent: Jul 24, 2008 4:22 PM What is important is not the number so much as if the paper is high retention and what is filtering speed. Whatman #4, if I remember correctly, is a medium fast paper, and should be ideal for filtering staining solution. Somewhere in the passage of time, I was told not to use paper that was really slow filtering speed and high retention for filtering dye solutions as the paper may retain too much of the dye needed. Now that could be an old wife's tale, but to this day I always use a medium fast paper. I also purchase something other than Whatman, as companies sell equivalents for a much cheaper price. Whatman 54 should also work. Big companies like ThermoScientific give the equivalents for their brand as compared to Whatman papers and are certainly more economical. Gayle M. Callis HTL/HT/MT(ASCP) ----- Original Message ----- From: "Myl?ne de Champlain" To: Sent: Wednesday, July 23, 2008 4:31 PM Subject: [Histonet] Filter paper Hi, To filter blue celestin, is what I have to use a filter paper number 1 or 4? Thank! Myl?ne de Champlain _________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sent via BlackBerry by AT&T Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------ The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From JPCOLEMA <@t> sentara.com Mon Jul 28 09:26:21 2008 From: JPCOLEMA <@t> sentara.com (JOHN P COLEMAN) Date: Mon Jul 28 09:26:35 2008 Subject: [Histonet] Grasshopper Message-ID: It would depend on the species of grasshopper, and the variety of cucumber, but not so much on the type of wood from which the leg was made. Since this is histo specific can we assume logwood? John P.J. Coleman HT(ASCP)QIHC Clinical Specialist Anatomic Pathology Sentara Laboratory Services Cell/Voicemail(757)335-2159 pager: (757)456-6695 office:-(757)388-3295 From Stacy_McLaughlin <@t> cooley-dickinson.org Mon Jul 28 09:42:13 2008 From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin) Date: Mon Jul 28 09:42:23 2008 Subject: [Histonet] Scale for weighing parathyroid Message-ID: Happy Monday Everyone, My hospital will soon be starting parathyroid surgeries. Can anyone recommend a good ultra-sensetive scale for weighing them? Thanks for your help. Stacy McLaughlin HT (ASCP) Lead Histologist Cooley Dickinson Hospital From jcline <@t> wchsys.org Mon Jul 28 10:52:57 2008 From: jcline <@t> wchsys.org (Joyce Cline) Date: Mon Jul 28 10:53:05 2008 Subject: [Histonet] Sakura VIP alarm In-Reply-To: Message-ID: <174778BBF27643EE8E912B8864AB433E@wchsys.org> The system you are talking about is a Sensaphone made by Phonetics,Inc. I have home phone numbers keyed in for the Sensaphone to call because there is no one in our lab at night. I am sure you can find them on the web, I have had this phone since 1997. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Christine Bark Sent: Friday, July 25, 2008 1:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura VIP alarm Happy Friday Histonetters!! I need to find an alarm system for my Sakura VIP E and VIP 5. Our current alarm system will become obsolete very soon as we are moving (too far apparently for main lab to hear). I've heard of a system that can page/phone someone when the alarm goes off but I can't seem to find out any more information. What is everyone else doing about alarms going off after-hours? Any and every response is welcome, including vendors. Thank you, Christine Bark HT(ASCP) CM Lead Histotech, Pathology Saddleback Memorial Medical Center 949-452-3548 cbark@memorialcare.org ____________________________________________________________________________ __ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From 1dpeterson <@t> meriter.com Mon Jul 28 11:21:28 2008 From: 1dpeterson <@t> meriter.com (Peterson, Dan) Date: Mon Jul 28 11:21:33 2008 Subject: [Histonet] Alcohol lamps Message-ID: <328CBAE62F31C642B422970E879DFADC01A80301@pcwex01> Fellow Histonetters, I am in disagreement with our lab's Safety officer with regards to alcohol lamps. We use them in our embedding area to keep our forceps clean. The officer says that they're a fire hazard (even though we've used them without incident for over 30 yrs). There are no flammable reagents (other than the alcohol in the lamp) near the embedding area. I know we could use the warming wells on the embedders, but try to find more that 1 pair of forceps that you like, or better yet, try to find a forceps that the tech before hasn't left paraffin all over it. (Yes, I am a fussy old goat, 27 yrs of Histo, with my 1 favorite pair that NOBODY touches) Petty issue? Are there others out there using lamps? I am willing to change if necessary (or so ordered), but would like to hear from those who do the work, not be told what to do by those who know nothing of the work. Thanks in advance!! Daniel R Peterson HT(ASCP) Histopathology Section Head Meriter Laboratories (608)-417-6557 1dpeterson@meriter.com CONFIDENTIALITY NOTICE: This message (including any attachments) is intended for the sole use of the individual and entity to whom it is addressed. This message may contain information that is confidential and is protected by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply email and then delete the message. Thank you. From JMacDonald <@t> mtsac.edu Mon Jul 28 11:24:58 2008 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Jul 28 11:25:03 2008 Subject: [Histonet] Alcohol lamps In-Reply-To: <328CBAE62F31C642B422970E879DFADC01A80301@pcwex01> Message-ID: I know of some labs that have switched to the Bacti incinerators for this purpose. "Peterson, Dan" <1dpeterson@meriter.com> Sent by: histonet-bounces@lists.utsouthwestern.edu 07/28/2008 09:23 AM To cc Subject [Histonet] Alcohol lamps Fellow Histonetters, I am in disagreement with our lab's Safety officer with regards to alcohol lamps. We use them in our embedding area to keep our forceps clean. The officer says that they're a fire hazard (even though we've used them without incident for over 30 yrs). There are no flammable reagents (other than the alcohol in the lamp) near the embedding area. I know we could use the warming wells on the embedders, but try to find more that 1 pair of forceps that you like, or better yet, try to find a forceps that the tech before hasn't left paraffin all over it. (Yes, I am a fussy old goat, 27 yrs of Histo, with my 1 favorite pair that NOBODY touches) Petty issue? Are there others out there using lamps? I am willing to change if necessary (or so ordered), but would like to hear from those who do the work, not be told what to do by those who know nothing of the work. Thanks in advance!! Daniel R Peterson HT(ASCP) Histopathology Section Head Meriter Laboratories (608)-417-6557 1dpeterson@meriter.com CONFIDENTIALITY NOTICE: This message (including any attachments) is intended for the sole use of the individual and entity to whom it is addressed. This message may contain information that is confidential and is protected by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply email and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cheastys <@t> svm.vetmed.wisc.edu Mon Jul 28 12:07:19 2008 From: cheastys <@t> svm.vetmed.wisc.edu (Sandra Cheasty) Date: Mon Jul 28 12:08:24 2008 Subject: [Histonet] Immuno Stainer Message-ID: Any suggestions re: an "open" Immunostainer that allows multiple detection kits, and also allows starting a new run before the previous run has completed? I work with veterinary diagnostic and research tissues. Thank you, Sandy UW-Madison From vapatpxs <@t> yahoo.com Mon Jul 28 12:44:45 2008 From: vapatpxs <@t> yahoo.com (Va Paula Sicurello) Date: Mon Jul 28 12:44:48 2008 Subject: [Histonet] Used embedding center Message-ID: <214245.32808.qm@web46112.mail.sp1.yahoo.com> Hi HistoPeople, I'm looking for a used, but one that works, embedding center. I'm in the process of adding a histology service to my EM and confocal lab and have everything except the embedding center. If you have one laying around in a closet somewhere, I'll pay for shipping. Please contact me if you have one that you'd like to get rid of. Paula Sicurello VA San Diego Healthcare System Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 From Janet.Bonner <@t> FLHOSP.ORG Mon Jul 28 12:43:47 2008 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Mon Jul 28 12:46:20 2008 Subject: [Histonet] Alcohol lamps References: <328CBAE62F31C642B422970E879DFADC01A80301@pcwex01> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2726@fhosxchmb006.ADVENTISTCORP.NET> ...and what about those xylene fumes that us old-timers cannot detect with our burned out noses? A flame will ignite xylene as well. Otherwise, we could all go back to "smokin' in da lab"!! Janet L. Bonner, HTL (ASCP) Pathology Laboratory Florida Hospital Winter Park janet.bonner@FLHOSP.org 407-646-7559 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Peterson, Dan Sent: Mon 7/28/2008 12:21 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Alcohol lamps Fellow Histonetters, I am in disagreement with our lab's Safety officer with regards to alcohol lamps. We use them in our embedding area to keep our forceps clean. The officer says that they're a fire hazard (even though we've used them without incident for over 30 yrs). There are no flammable reagents (other than the alcohol in the lamp) near the embedding area. I know we could use the warming wells on the embedders, but try to find more that 1 pair of forceps that you like, or better yet, try to find a forceps that the tech before hasn't left paraffin all over it. (Yes, I am a fussy old goat, 27 yrs of Histo, with my 1 favorite pair that NOBODY touches) Petty issue? Are there others out there using lamps? I am willing to change if necessary (or so ordered), but would like to hear from those who do the work, not be told what to do by those who know nothing of the work. Thanks in advance!! Daniel R Peterson HT(ASCP) Histopathology Section Head Meriter Laboratories (608)-417-6557 1dpeterson@meriter.com CONFIDENTIALITY NOTICE: This message (including any attachments) is intended for the sole use of the individual and entity to whom it is addressed. This message may contain information that is confidential and is protected by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply email and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From tjasper <@t> copc.net Mon Jul 28 13:05:51 2008 From: tjasper <@t> copc.net (Thomas Jasper) Date: Mon Jul 28 13:05:57 2008 Subject: [Histonet] Alcohol lamps References: <328CBAE62F31C642B422970E879DFADC01A80301@pcwex01> Message-ID: <90354A475B420441B2A0396E5008D4965E211F@copc-sbs.COPC.local> Hey Dan, Am compelled to respond as I know just what you mean about having a flame available. Back in the "good old days" about 10 years ago, at my previous employer, we were forced to get rid of our Bunsen burners. These of course provided a flame just like your alcohol lamps. I do have to say they may have been a bit more dangerous as they were fed by a tube of gas...I'm sure you get the picture. Anyway, not only do I agree with you about one forceps and the paraffin coating deal. I also liked the fact that the flame eliminated any residuals, between blocks. I always thought that was a GLP re: cross contamination, floaters, etc. However overall lab safety trumped my GLP reasons. And as you've stated...no accidents, ever to my knowledge, in my old lab. I have to admit, when we lost the burners I had a sad feeling. It may seem a bit silly, but I felt it really changed how efficiently and effectively we could get our embedding done. Eventually we obtained some bacti-incinerators (I think this has been mentioned already). They're ok, but you've really got to watch it as the forceps heat so quickly you'll burn your hand. It'd be nice if you could get something like a bacti-incinerator at a few thousand degrees cooler!? So Dan, not that my story helps your cause any, but at least you know I sympathize. I do have the feeling your safety officer is probably going to be able to throw his/her weight around with you on this one. Where I'm working now, we don't have bacti-incinerators. We only have the warming wells, and I try and rotate 2 or 3 of my "favorite" pairs of forceps when embedding. I know I'd be devastated if there'd ever been an accident with flammables (as anyone would). And I'm sure your safety officer will make the point about paraffin being flammable near your alcohol lamp. I believe safety officers are forced to look at things a certain way. There's no way he/she is ever going to understand where you're coming from and probably has a hard time understanding what your problem is. But it's not their job to get your job done...what to do? Think you'll have to suck it up and say good bye to your trusty lamps. Fellow former fan and friend of fire, Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, OR 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Peterson, Dan Sent: Monday, July 28, 2008 9:21 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Alcohol lamps Fellow Histonetters, I am in disagreement with our lab's Safety officer with regards to alcohol lamps. We use them in our embedding area to keep our forceps clean. The officer says that they're a fire hazard (even though we've used them without incident for over 30 yrs). There are no flammable reagents (other than the alcohol in the lamp) near the embedding area. I know we could use the warming wells on the embedders, but try to find more that 1 pair of forceps that you like, or better yet, try to find a forceps that the tech before hasn't left paraffin all over it. (Yes, I am a fussy old goat, 27 yrs of Histo, with my 1 favorite pair that NOBODY touches) Petty issue? Are there others out there using lamps? I am willing to change if necessary (or so ordered), but would like to hear from those who do the work, not be told what to do by those who know nothing of the work. Thanks in advance!! Daniel R Peterson HT(ASCP) Histopathology Section Head Meriter Laboratories (608)-417-6557 1dpeterson@meriter.com CONFIDENTIALITY NOTICE: This message (including any attachments) is intended for the sole use of the individual and entity to whom it is addressed. This message may contain information that is confidential and is protected by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply email and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Gloria.A.Okland <@t> questdiagnostics.com Mon Jul 28 13:20:34 2008 From: Gloria.A.Okland <@t> questdiagnostics.com (Okland, Gloria A) Date: Mon Jul 28 13:21:03 2008 Subject: [Histonet] Blue Zones Message-ID: Hi All, We have been seeing a very random appearance of Blue Zones on our H&E slides. It affects one section, but not the next in serial sections and if we restain the slides, they look better, and it seems to just be the GI biopsies that we have trouble with. It seems like the tissue is not being stained by the eosin in these areas. The slides are deparraffinized and hydrated, and the hematoxylin stain looks ok, it is just the Eosin. Any suggestions that you may have, are greatly appreciated, this have been an ongoing problem for some time now. Thanks Gloria Gloria Okland HT (ASCP) Histology Supervisor Quest Diagnostics 4770 Regent Blvd Irving, TX 75063 972-536-8820 972-916-3539 fax ------------------------------------------ The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. From sharon.osborn <@t> comcast.net Mon Jul 28 13:29:51 2008 From: sharon.osborn <@t> comcast.net (sharon.osborn@comcast.net) Date: Mon Jul 28 13:29:58 2008 Subject: [Histonet] alcohol lamps Message-ID: <072820081829.13697.488E101F00063A1F000035812215593414029D010D9C01D202019D0E089C@comcast.net> Dan, About ten years ago, in setting up a new histology laboratory, management, techs, pathologists and I had this discussion. Safety regulations require no open flames in a laboratory area due to the explosion capabilities as well as the fire hazards. I had already told the techs we would not be using the alcohol lamps and we did not install gas lines in the new facility build out. This was already determined by the pathology group before the building commenced. There were 2-3 older techs (like me) who preferred to use the flames to clear the forceps of contamination from tissue and paraffin before embedding a new block. Yes, it is faster, however, very dangerous. i, too had done it for millenimum as you w/o risk. Management thought they would get on the good side of the techs and surprise them with alcohol burners. I discovered this before the techs came in and removed the alcohol lamps. Thank goodness, the head pathologist backed me up. As a post-doc he had a horrific personal experience in having an alcohol lamp ignite his lab coat when he accidentally brushed his sleeve across it. He understood the implications of the open flame in the lab. Also, where there are alcohols, xylenes or other similar flammable solvents around, the vapors could accidentally build up to create an easily combustible situation. So, the techs learned to use lots of Kim Wipes on the forceps before placing them in the warmers and to use Q-tips to k eep the warmers clear of tissue pieces that could contaminate a block. And, over time, it really does not slow you down that much in the embedding process. It is a change to not use the alcohol burners; however, the safety benefits far outweigh the hazards imposed by the open flame. And, as is often the case, the embedding person may be the only one in the laboratory EARLY in the morning and no one would know there was a fire until it might be too late. Sharon Osborn, BS, HT(ASCP) C Lab Vision Fremont, CA Date: Mon, 28 Jul 2008 11:21:28 -0500 From: "Peterson, Dan" <1dpeterson@meriter.com> Subject: [Histonet] Alcohol lamps To: Message-ID: <328CBAE62F31C642B422970E879DFADC01A80301@pcwex01> Content-Type: text/plain; charset="us-ascii" Fellow Histonetters, I am in disagreement with our lab's Safety officer with regards to alcohol lamps. We use them in our embedding area to keep our forceps clean. The officer says that they're a fire hazard (even though we've used them without incident for over 30 yrs). There are no flammable reagents (other than the alcohol in the lamp) near the embedding area. I know we could use the warming wells on the embedders, but try to find more that 1 pair of forceps that you like, or better yet, try to find a forceps that the tech before hasn't left paraffin all over it. (Yes, I am a fussy old goat, 27 yrs of Histo, with my 1 favorite pair that NOBODY touches) Petty issue? Are there others out there using lamps? I am willing to change if necessary (or so ordered), but would like to hear from those who do the work, not be told what to do by those who know nothing of the work. Thanks in advance!! Daniel R Peterson HT(ASCP) Histopathology Section Head Meriter Laboratories (608)-417-6557 1dpeterson@meriter.com From gayle.callis <@t> bresnan.net Mon Jul 28 13:48:33 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Mon Jul 28 13:48:38 2008 Subject: [Histonet] Alcohol lamps References: <328CBAE62F31C642B422970E879DFADC01A80301@pcwex01> Message-ID: <001b01c8f0e2$89846710$6401a8c0@Sunney> We don't use open flames i.e. alcohol burners anymore, due to fire hazard (one could tip it over, spill alcohol and burn!). The forcep tips, if held too long in the flame, can also burn the tissue. We use heated embedding center wells but purchase longer forceps i.e. ergonomically acceptable You can buy forceps with L,hockey stick (packing forceps) shape to them, or buy straight with both wider and narrower, but NOT sharp pointed ends. We keep more one forceps to choose from in our wells, users can chose their favorites out this selection . We like longer, slender forceps for following reasons: 1. do not burn fingers during embedding 2. manipulate small tissues easily Try Arista Surgical Supply (www.aristasurgical.com , EMS, and Henry Schein (dentristy forceps, packing style) - none of these companies will break the bank and Arista has a HUGE selection of all types of forceps. As for paraffin on forceps, after embedding, lay them in cassette holding area on paper towel to soak up melted paraffin. Wipe them off while hot, return to wells when needed. In our lab, we have as many as 10 or more different people embedding their samples and THEY learn to do this very quickly. No moe complaining about paraffin all over the forceps this way. One can also try the metal mold holding area - where temp may be hot enought to melt the paraffin. We put forceps through cleaning cycle on processor and on occasion, a good paraffin removing solvent soak, rinsed off with alcohol or even a superhot water wash. If you have your favorite forceps, then others must have favorites too? Why not supply each person with their favorite too - then you are a hero instead of a "fussy old goat"? Gayle M. Callis HTL/HT/MT(ASCP) ----- Original Message ----- From: "Peterson, Dan" <1dpeterson@meriter.com> To: Sent: Monday, July 28, 2008 10:21 AM Subject: [Histonet] Alcohol lamps Fellow Histonetters, I am in disagreement with our lab's Safety officer with regards to alcohol lamps. We use them in our embedding area to keep our forceps clean. The officer says that they're a fire hazard (even though we've used them without incident for over 30 yrs). There are no flammable reagents (other than the alcohol in the lamp) near the embedding area. I know we could use the warming wells on the embedders, but try to find more that 1 pair of forceps that you like, or better yet, try to find a forceps that the tech before hasn't left paraffin all over it. (Yes, I am a fussy old goat, 27 yrs of Histo, with my 1 favorite pair that NOBODY touches) Petty issue? Are there others out there using lamps? I am willing to change if necessary (or so ordered), but would like to hear from those who do the work, not be told what to do by those who know nothing of the work. Thanks in advance!! Daniel R Peterson HT(ASCP) Histopathology Section Head Meriter Laboratories (608)-417-6557 1dpeterson@meriter.com CONFIDENTIALITY NOTICE: This message (including any attachments) is intended for the sole use of the individual and entity to whom it is addressed. This message may contain information that is confidential and is protected by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply email and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Mon Jul 28 14:01:12 2008 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Mon Jul 28 14:01:17 2008 Subject: [Histonet] Alcohol lamps In-Reply-To: <328CBAE62F31C642B422970E879DFADC01A80301@pcwex01> Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835C40@LSRIEXCH1.lsmaster.lifespan.org> Another fussy old goat here! Alcohol lamps have served me well for 40 years, and I don't plan on changing unless they threaten to make me go back to using an autotechnicon and embedding with a pitcher of wax! Paul M. From Jackie.O'Connor <@t> abbott.com Mon Jul 28 14:41:22 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Mon Jul 28 14:41:43 2008 Subject: [Histonet] alcohol lamps In-Reply-To: <072820081829.13697.488E101F00063A1F000035812215593414029D010D9C01D202019D0E089C@comcast.net> Message-ID: In 1971 in Chicago, there was a fire in a histology lab in Chicago because of an alcohol lamp. The ignited lamp was knocked off the bench, fell to the floor, jar broke, all the spilled alcohol was ignited over a large area which went on to ignite another open container of another flammable on the floor (cleaning xylene, I think). A terrible fire resulted. Two techs were badly burned, since there was only one door to the lab, they had to run through the fire to get out (lab was on the 9th floor). I don't know who they were, or how they are now - but I was the technician who went to work there after the fire. Jackie O' sharon.osborn@comcast.net Sent by: histonet-bounces@lists.utsouthwestern.edu 07/28/2008 01:29 PM To histonet@pathology.swmed.edu cc Subject [Histonet] alcohol lamps Dan, About ten years ago, in setting up a new histology laboratory, management, techs, pathologists and I had this discussion. Safety regulations require no open flames in a laboratory area due to the explosion capabilities as well as the fire hazards. I had already told the techs we would not be using the alcohol lamps and we did not install gas lines in the new facility build out. This was already determined by the pathology group before the building commenced. There were 2-3 older techs (like me) who preferred to use the flames to clear the forceps of contamination from tissue and paraffin before embedding a new block. Yes, it is faster, however, very dangerous. i, too had done it for millenimum as you w/o risk. Management thought they would get on the good side of the techs and surprise them with alcohol burners. I discovered this before the techs came in and removed the alcohol lamps. Thank goodness, the head pathologist backed me up. As a post-doc he had a horrific personal experience in having an alcohol lamp ignite his lab coat when he accidentally brushed his sleeve across it. He understood the implications of the open flame in the lab. Also, where there are alcohols, xylenes or other similar flammable solvents around, the vapors could accidentally build up to create an easily combustible situation. So, the techs learned to use lots of Kim Wipes on the forceps before placing them in the warmers and to use Q-tips to k eep the warmers clear of tissue pieces that could contaminate a block. And, over time, it really does not slow you down that much in the embedding process. It is a change to not use the alcohol burners; however, the safety benefits far outweigh the hazards imposed by the open flame. And, as is often the case, the embedding person may be the only one in the laboratory EARLY in the morning and no one would know there was a fire until it might be too late. Sharon Osborn, BS, HT(ASCP) C Lab Vision Fremont, CA Date: Mon, 28 Jul 2008 11:21:28 -0500 From: "Peterson, Dan" <1dpeterson@meriter.com> Subject: [Histonet] Alcohol lamps To: Message-ID: <328CBAE62F31C642B422970E879DFADC01A80301@pcwex01> Content-Type: text/plain; charset="us-ascii" Fellow Histonetters, I am in disagreement with our lab's Safety officer with regards to alcohol lamps. We use them in our embedding area to keep our forceps clean. The officer says that they're a fire hazard (even though we've used them without incident for over 30 yrs). There are no flammable reagents (other than the alcohol in the lamp) near the embedding area. I know we could use the warming wells on the embedders, but try to find more that 1 pair of forceps that you like, or better yet, try to find a forceps that the tech before hasn't left paraffin all over it. (Yes, I am a fussy old goat, 27 yrs of Histo, with my 1 favorite pair that NOBODY touches) Petty issue? Are there others out there using lamps? I am willing to change if necessary (or so ordered), but would like to hear from those who do the work, not be told what to do by those who know nothing of the work. Thanks in advance!! Daniel R Peterson HT(ASCP) Histopathology Section Head Meriter Laboratories (608)-417-6557 1dpeterson@meriter.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Mon Jul 28 14:43:50 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Mon Jul 28 14:43:54 2008 Subject: [Histonet] Re: Scale for weighing parathyroid Message-ID: Stacy McLaughlin HT (ASCP) at Cooley Dickinson Hospital in Northampton MA asks: >>My hospital will soon be starting parathyroid surgeries. Can anyone recommend a good ultra-sensitive scale for weighing them?<< I asked this question several days ago, here and on the pathology list, and got no responses. I've been researching the question today, and I've found a balance that MAY be suitable. What you need is a balance that will go up to about 100 grams, in increments of 10 milligrams. (Normal parathyroids run a few tens of milligrams, while adenomas can go well over a gram.) I called Fisher Scientific customer service at 800-766-7000. Their model S40152 (in the online catalog) has been replaced by their S94792B. These balances are made for Fisher by Ohaus. The Fisher representative said that the S94792B, also called the SLF 152, is a simplified version of Ohaus's Ohaus Valor 3000 Xtreme V31XH202, which costs about $400. Fisher sells the simpler model for $228. The Ohaus customer service person (at 800-526-0659, 2, 1) wasn't familiar with the balances Ohaus makes for Fisher. Before you go buying anything, check if your hospital pharmacy still has a balance. That's what I've always done with parathyroid adenomas - not very frequent specimens - but these days a lot of small hospital pharmacies no longer compound. Can anyone else on Histonet weigh in (sorry about that!) with some recommendations? Bob Richmond Samurai Pathologist Knoxville TN From Barry.R.Rittman <@t> uth.tmc.edu Mon Jul 28 14:49:14 2008 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Mon Jul 28 14:49:21 2008 Subject: [Histonet] Alcohol lamps References: <328CBAE62F31C642B422970E879DFADC01A80301@pcwex01> Message-ID: Like most things that are potentially dangerous, alcohol lamp may be a hazard. It is however the person using the lamp that is often the greatest hazard. I have seen a person stick them selves accidentally in the hand with a pencil. Should we then ban all pointed objects from the lab as they are potentially dangerous? I believe that if the individuals using the lamps are trained and adhere to appropriate safety procedures there is no problem. I do feel on the other hand, however that some of the alcohol lamp designs are not as stable as they should be. This can however be corrected by placing several small glass beads in the alcohol reservoir to make them less likely to tip over. Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Peterson, Dan Sent: Mon 7/28/2008 11:21 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Alcohol lamps Fellow Histonetters, I am in disagreement with our lab's Safety officer with regards to alcohol lamps. We use them in our embedding area to keep our forceps clean. The officer says that they're a fire hazard (even though we've used them without incident for over 30 yrs). There are no flammable reagents (other than the alcohol in the lamp) near the embedding area. I know we could use the warming wells on the embedders, but try to find more that 1 pair of forceps that you like, or better yet, try to find a forceps that the tech before hasn't left paraffin all over it. (Yes, I am a fussy old goat, 27 yrs of Histo, with my 1 favorite pair that NOBODY touches) Petty issue? Are there others out there using lamps? I am willing to change if necessary (or so ordered), but would like to hear from those who do the work, not be told what to do by those who know nothing of the work. Thanks in advance!! Daniel R Peterson HT(ASCP) Histopathology Section Head Meriter Laboratories (608)-417-6557 1dpeterson@meriter.com CONFIDENTIALITY NOTICE: This message (including any attachments) is intended for the sole use of the individual and entity to whom it is addressed. This message may contain information that is confidential and is protected by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply email and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asmith <@t> mail.barry.edu Mon Jul 28 15:25:24 2008 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Mon Jul 28 15:26:57 2008 Subject: [Histonet] alcohol lamps In-Reply-To: References: <072820081829.13697.488E101F00063A1F000035812215593414029D010D9C01D202019D0E089C@comcast.net> Message-ID: I have worried about this possibility. This is why I use a stamped steel alcohol lamp. The larger fire was the result of poor communication. Before lighting, I announce: I am about to strike a match. All solvents must be covered! (By the way, where was that lab's fire extinguisher?) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Monday, July 28, 2008 3:41 PM To: sharon.osborn@comcast.net Cc: histonet-bounces@lists.utsouthwestern.edu; histonet@pathology.swmed.edu Subject: Re: [Histonet] alcohol lamps In 1971 in Chicago, there was a fire in a histology lab in Chicago because of an alcohol lamp. The ignited lamp was knocked off the bench, fell to the floor, jar broke, all the spilled alcohol was ignited over a large area which went on to ignite another open container of another flammable on the floor (cleaning xylene, I think). A terrible fire resulted. Two techs were badly burned, since there was only one door to the lab, they had to run through the fire to get out (lab was on the 9th floor). I don't know who they were, or how they are now - but I was the technician who went to work there after the fire. Jackie O' sharon.osborn@comcast.net Sent by: histonet-bounces@lists.utsouthwestern.edu 07/28/2008 01:29 PM To histonet@pathology.swmed.edu cc Subject [Histonet] alcohol lamps Dan, About ten years ago, in setting up a new histology laboratory, management, techs, pathologists and I had this discussion. Safety regulations require no open flames in a laboratory area due to the explosion capabilities as well as the fire hazards. I had already told the techs we would not be using the alcohol lamps and we did not install gas lines in the new facility build out. This was already determined by the pathology group before the building commenced. There were 2-3 older techs (like me) who preferred to use the flames to clear the forceps of contamination from tissue and paraffin before embedding a new block. Yes, it is faster, however, very dangerous. i, too had done it for millenimum as you w/o risk. Management thought they would get on the good side of the techs and surprise them with alcohol burners. I discovered this before the techs came in and removed the alcohol lamps. Thank goodness, the head pathologist backed me up. As a post-doc he had a horrific personal experience in having an alcohol lamp ignite his lab coat when he accidentally brushed his sleeve across it. He understood the implications of the open flame in the lab. Also, where there are alcohols, xylenes or other similar flammable solvents around, the vapors could accidentally build up to create an easily combustible situation. So, the techs learned to use lots of Kim Wipes on the forceps before placing them in the warmers and to use Q-tips to k eep the warmers clear of tissue pieces that could contaminate a block. And, over time, it really does not slow you down that much in the embedding process. It is a change to not use the alcohol burners; however, the safety benefits far outweigh the hazards imposed by the open flame. And, as is often the case, the embedding person may be the only one in the laboratory EARLY in the morning and no one would know there was a fire until it might be too late. Sharon Osborn, BS, HT(ASCP) C Lab Vision Fremont, CA Date: Mon, 28 Jul 2008 11:21:28 -0500 From: "Peterson, Dan" <1dpeterson@meriter.com> Subject: [Histonet] Alcohol lamps To: Message-ID: <328CBAE62F31C642B422970E879DFADC01A80301@pcwex01> Content-Type: text/plain; charset="us-ascii" Fellow Histonetters, I am in disagreement with our lab's Safety officer with regards to alcohol lamps. We use them in our embedding area to keep our forceps clean. The officer says that they're a fire hazard (even though we've used them without incident for over 30 yrs). There are no flammable reagents (other than the alcohol in the lamp) near the embedding area. I know we could use the warming wells on the embedders, but try to find more that 1 pair of forceps that you like, or better yet, try to find a forceps that the tech before hasn't left paraffin all over it. (Yes, I am a fussy old goat, 27 yrs of Histo, with my 1 favorite pair that NOBODY touches) Petty issue? Are there others out there using lamps? I am willing to change if necessary (or so ordered), but would like to hear from those who do the work, not be told what to do by those who know nothing of the work. Thanks in advance!! Daniel R Peterson HT(ASCP) Histopathology Section Head Meriter Laboratories (608)-417-6557 1dpeterson@meriter.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Mon Jul 28 15:30:42 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Mon Jul 28 15:31:00 2008 Subject: [Histonet] alcohol lamps In-Reply-To: Message-ID: Probably outside the door in the hall. Remember, it was 1971 - - we could smoke and eat in the labs then, there were no safety rules. Heck, this lab only had one door. By the time I went to work there, they had made a second egress. "Smith, Allen" 07/28/2008 03:25 PM To 'Jackie M O'Connor' cc "'histonet@lists.utsouthwestern.edu'" Subject RE: [Histonet] alcohol lamps I have worried about this possibility. This is why I use a stamped steel alcohol lamp. The larger fire was the result of poor communication. Before lighting, I announce: I am about to strike a match. All solvents must be covered! (By the way, where was that lab's fire extinguisher?) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Monday, July 28, 2008 3:41 PM To: sharon.osborn@comcast.net Cc: histonet-bounces@lists.utsouthwestern.edu; histonet@pathology.swmed.edu Subject: Re: [Histonet] alcohol lamps In 1971 in Chicago, there was a fire in a histology lab in Chicago because of an alcohol lamp. The ignited lamp was knocked off the bench, fell to the floor, jar broke, all the spilled alcohol was ignited over a large area which went on to ignite another open container of another flammable on the floor (cleaning xylene, I think). A terrible fire resulted. Two techs were badly burned, since there was only one door to the lab, they had to run through the fire to get out (lab was on the 9th floor). I don't know who they were, or how they are now - but I was the technician who went to work there after the fire. Jackie O' sharon.osborn@comcast.net Sent by: histonet-bounces@lists.utsouthwestern.edu 07/28/2008 01:29 PM To histonet@pathology.swmed.edu cc Subject [Histonet] alcohol lamps Dan, About ten years ago, in setting up a new histology laboratory, management, techs, pathologists and I had this discussion. Safety regulations require no open flames in a laboratory area due to the explosion capabilities as well as the fire hazards. I had already told the techs we would not be using the alcohol lamps and we did not install gas lines in the new facility build out. This was already determined by the pathology group before the building commenced. There were 2-3 older techs (like me) who preferred to use the flames to clear the forceps of contamination from tissue and paraffin before embedding a new block. Yes, it is faster, however, very dangerous. i, too had done it for millenimum as you w/o risk. Management thought they would get on the good side of the techs and surprise them with alcohol burners. I discovered this before the techs came in and removed the alcohol lamps. Thank goodness, the head pathologist backed me up. As a post-doc he had a horrific personal experience in having an alcohol lamp ignite his lab coat when he accidentally brushed his sleeve across it. He understood the implications of the open flame in the lab. Also, where there are alcohols, xylenes or other similar flammable solvents around, the vapors could accidentally build up to create an easily combustible situation. So, the techs learned to use lots of Kim Wipes on the forceps before placing them in the warmers and to use Q-tips to k eep the warmers clear of tissue pieces that could contaminate a block. And, over time, it really does not slow you down that much in the embedding process. It is a change to not use the alcohol burners; however, the safety benefits far outweigh the hazards imposed by the open flame. And, as is often the case, the embedding person may be the only one in the laboratory EARLY in the morning and no one would know there was a fire until it might be too late. Sharon Osborn, BS, HT(ASCP) C Lab Vision Fremont, CA Date: Mon, 28 Jul 2008 11:21:28 -0500 From: "Peterson, Dan" <1dpeterson@meriter.com> Subject: [Histonet] Alcohol lamps To: Message-ID: <328CBAE62F31C642B422970E879DFADC01A80301@pcwex01> Content-Type: text/plain; charset="us-ascii" Fellow Histonetters, I am in disagreement with our lab's Safety officer with regards to alcohol lamps. We use them in our embedding area to keep our forceps clean. The officer says that they're a fire hazard (even though we've used them without incident for over 30 yrs). There are no flammable reagents (other than the alcohol in the lamp) near the embedding area. I know we could use the warming wells on the embedders, but try to find more that 1 pair of forceps that you like, or better yet, try to find a forceps that the tech before hasn't left paraffin all over it. (Yes, I am a fussy old goat, 27 yrs of Histo, with my 1 favorite pair that NOBODY touches) Petty issue? Are there others out there using lamps? I am willing to change if necessary (or so ordered), but would like to hear from those who do the work, not be told what to do by those who know nothing of the work. Thanks in advance!! Daniel R Peterson HT(ASCP) Histopathology Section Head Meriter Laboratories (608)-417-6557 1dpeterson@meriter.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SDrew <@t> uwhealth.org Mon Jul 28 15:34:31 2008 From: SDrew <@t> uwhealth.org (Drew Sally A.) Date: Mon Jul 28 15:34:37 2008 Subject: [Histonet] Human Herpes Virus 6 Message-ID: <3F328377AF4E23438E78234752652CE105D5276A@uwhis-xchng7.uwhis.hosp.wisc.edu> I've been asked to find out if there are any reference labs "out there" that offer HHV-6? Thank you for any leads offered! Sally Ann Drew, MT (ASCP) sdrew@uwhealth.org IHC/ISH Lab DB1-223, Mail Code 3224 600 Highland Ave. Madison, WI 53792 Phone (608) 265-6596 Fax (608) 262-7174 From victor <@t> pathology.washington.edu Mon Jul 28 15:43:28 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Mon Jul 28 15:43:42 2008 Subject: [Histonet] alcohol lamps In-Reply-To: References: Message-ID: <488E2F70.5050103@pathology.washington.edu> What a coincidence that it happened 100 years later in Chicago, can't blame it on the cow this time. http://www.thechicagofire.com/ Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Jackie M O'Connor wrote: > In 1971 in Chicago, there was a fire in a histology lab in Chicago because > of an alcohol lamp. The ignited lamp was knocked off the bench, fell to > the floor, jar broke, all the spilled alcohol was ignited over a large > area which went on to ignite another open container of another flammable > on the floor (cleaning xylene, I think). A terrible fire resulted. Two > techs were badly burned, since there was only one door to the lab, they > had to run through the fire to get out (lab was on the 9th floor). I > don't know who they were, or how they are now - but I was the technician > who went to work there after the fire. > Jackie O' > > > > > sharon.osborn@comcast.net > Sent by: histonet-bounces@lists.utsouthwestern.edu > 07/28/2008 01:29 PM > > To > histonet@pathology.swmed.edu > cc > > Subject > [Histonet] alcohol lamps > > > > > > > > Dan, > About ten years ago, in setting up a new histology laboratory, > management, techs, pathologists and I had this discussion. Safety > regulations require no open flames in a laboratory area due to the > explosion capabilities as well as the fire hazards. I had already told > the techs we would not be using the alcohol lamps and we did not install > gas lines in the new facility build out. This was already determined by > the pathology group before the building commenced. > There were 2-3 older techs (like me) who preferred to use the flames to > clear the forceps of contamination from tissue and paraffin before > embedding a new block. Yes, it is faster, however, very dangerous. i, > too had done it for millenimum as you w/o risk. Management thought they > would get on the good side of the techs and surprise them with alcohol > burners. I discovered this before the techs came in and removed the > alcohol lamps. Thank goodness, the head pathologist backed me up. As a > post-doc he had a horrific personal experience in having an alcohol lamp > ignite his lab coat when he accidentally brushed his sleeve across it. He > understood the implications of the open flame in the lab. Also, where > there are alcohols, xylenes or other similar flammable solvents around, > the vapors could accidentally build up to create an easily combustible > situation. So, the techs learned to use lots of Kim Wipes on the forceps > before placing them in the warmers and to use Q-tips to k > eep the warmers clear of tissue pieces that could contaminate a block. > And, over time, it really does not slow you down that much in the > embedding process. > It is a change to not use the alcohol burners; however, the safety > benefits far outweigh the hazards imposed by the open flame. And, as is > often the case, the embedding person may be the only one in the laboratory > EARLY in the morning and no one would know there was a fire until it might > be too late. > > Sharon Osborn, BS, HT(ASCP) C > Lab Vision > Fremont, CA > > > > Date: Mon, 28 Jul 2008 11:21:28 -0500 > From: "Peterson, Dan" <1dpeterson@meriter.com> > Subject: [Histonet] Alcohol lamps > To: > Message-ID: <328CBAE62F31C642B422970E879DFADC01A80301@pcwex01> > Content-Type: text/plain; charset="us-ascii" > > Fellow Histonetters, > I am in disagreement with our lab's Safety officer with regards to > alcohol lamps. We use them in our embedding area to keep our forceps > clean. > The officer says that they're a fire hazard (even though we've used them > without incident for over 30 yrs). There are no flammable reagents > (other than the alcohol in the lamp) near the embedding area. I know we > could use the warming wells on the embedders, but try to find more that > 1 pair of forceps that you like, or better yet, try to find a forceps > that the tech before hasn't left paraffin all over it. (Yes, I am a > fussy old goat, 27 yrs of Histo, with my 1 favorite pair that NOBODY > touches) > Petty issue? Are there others out there using lamps? I am willing to > change if necessary (or so ordered), but would like to hear from those > who do the work, not be told what to do by those who know nothing of the > work. Thanks in advance!! > > Daniel R Peterson HT(ASCP) > Histopathology Section Head > Meriter Laboratories > (608)-417-6557 > 1dpeterson@meriter.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tbritten <@t> aol.com Mon Jul 28 15:43:46 2008 From: tbritten <@t> aol.com (tbritten@aol.com) Date: Mon Jul 28 15:44:01 2008 Subject: [Histonet] Human Herpes Virus 6 Message-ID: <1492695284-1217277835-cardhu_decombobulator_blackberry.rim.net-176270595-@bxe197.bisx.prod.on.blackberry> Hi sally; we offer the slides if that helps. I don't want this to be a commercial for us. Would you like to contact me off line? Regards tom britten ------Original Message------ From: Drew Sally A. Sender: histonet-bounces@lists.utsouthwestern.edu To: Histonet Sent: Jul 28, 2008 4:34 PM Subject: [Histonet] Human Herpes Virus 6 I've been asked to find out if there are any reference labs "out there" that offer HHV-6? Thank you for any leads offered! Sally Ann Drew, MT (ASCP) sdrew@uwhealth.org IHC/ISH Lab DB1-223, Mail Code 3224 600 Highland Ave. Madison, WI 53792 Phone (608) 265-6596 Fax (608) 262-7174 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sent from my Verizon Wireless BlackBerry From Robert.Fauck <@t> ccdhb.org.nz Mon Jul 28 17:55:35 2008 From: Robert.Fauck <@t> ccdhb.org.nz (Robert Fauck [CCDHB]) Date: Mon Jul 28 17:56:34 2008 Subject: [Histonet] Creutz Jacob Disease Fitxtion, Hanling, and disposal of infected tissue Message-ID: <5587F098B7C4EE489C8BE204E9565548248D12@wn0nteml05.hiq.net.nz> Hi Everybody Can somebody give me an update on CJD fixation, handling and disposal of CJD infected ( or suspected) tissue, please. Thanking you in advance for your expertise and help. Regards, Robert Fauck Wellington Hospital, New Zealand This email or attachment(s) may contain confidential or legally privileged information intended for the sole use of the addressee(s). Any use, redistribution, disclosure, or reproduction of this message, except as intended, is prohibited. If you received this email in error, please notify the sender and remove all copies of the message, including any attachments. Any views or opinions expressed in this email (unless otherwise stated) may not represent those of Capital & Coast District Health Board. http://www.ccdhb.org.nz (1C_S1) No Viruses were detected in this message. HealthIntelligence eMail Filter Service From barbaraaalbert <@t> yahoo.com Mon Jul 28 18:08:46 2008 From: barbaraaalbert <@t> yahoo.com (Barbara Albert) Date: Mon Jul 28 18:08:51 2008 Subject: [Histonet] Sodium barbital buffer substitute Message-ID: <356339.21004.qm@web63707.mail.re1.yahoo.com> Hi all, I've been asked to post this question to the list.? What do you use as a substitute for Sodium barbitol buffer in your muscle histochemistries?? ? Thanks, Barbara Albert UCSF Medical Center San Francisco From AnthonyH <@t> chw.edu.au Mon Jul 28 18:32:47 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Jul 28 18:33:04 2008 Subject: [Histonet] Alcohol lamps In-Reply-To: <328CBAE62F31C642B422970E879DFADC01A80301@pcwex01> Message-ID: There have been incidents of fires at embedding benches, the wax can ignite (i.e. wax candles). Also alcohol fires are invisible (refer to your famous NASCARs). There have been at least 4 that I am aware of in Australia. It is not something that would be well publicised since it is the embarrassment that tends to keep it quiet! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Peterson, Dan Sent: Tuesday, 29 July 2008 2:21 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Alcohol lamps Fellow Histonetters, I am in disagreement with our lab's Safety officer with regards to alcohol lamps. We use them in our embedding area to keep our forceps clean. The officer says that they're a fire hazard (even though we've used them without incident for over 30 yrs). There are no flammable reagents (other than the alcohol in the lamp) near the embedding area. I know we could use the warming wells on the embedders, but try to find more that 1 pair of forceps that you like, or better yet, try to find a forceps that the tech before hasn't left paraffin all over it. (Yes, I am a fussy old goat, 27 yrs of Histo, with my 1 favorite pair that NOBODY touches) Petty issue? Are there others out there using lamps? I am willing to change if necessary (or so ordered), but would like to hear from those who do the work, not be told what to do by those who know nothing of the work. Thanks in advance!! Daniel R Peterson HT(ASCP) Histopathology Section Head Meriter Laboratories (608)-417-6557 1dpeterson@meriter.com CONFIDENTIALITY NOTICE: This message (including any attachments) is intended for the sole use of the individual and entity to whom it is addressed. This message may contain information that is confidential and is protected by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply email and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Mon Jul 28 18:39:07 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Jul 28 18:39:15 2008 Subject: [Histonet] Sodium barbital buffer substitute In-Reply-To: <356339.21004.qm@web63707.mail.re1.yahoo.com> Message-ID: Use an acetate buffer. It works well for ATPases and acid phosphatases. DO NOT use for esterases (use phosphate) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Barbara Albert Sent: Tuesday, 29 July 2008 9:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sodium barbital buffer substitute Hi all, I've been asked to post this question to the list.? What do you use as a substitute for Sodium barbitol buffer in your muscle histochemistries?? ? Thanks, Barbara Albert UCSF Medical Center San Francisco _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From hilda_1075 <@t> yahoo.com Mon Jul 28 23:44:03 2008 From: hilda_1075 <@t> yahoo.com (shazana hilda) Date: Mon Jul 28 23:44:07 2008 Subject: [Histonet] Protocol for polyester wax embedding using Methacarn-fixed tissue Message-ID: <811738.21252.qm@web38805.mail.mud.yahoo.com> Dear Histonetters, ? I'll be?processing tissue manually from Methacarn-fixed tissue using polyester wax. As?a first-timer i'm not familiar with?the protocol using polyester wax. They're few journals mentioned variety of protocols. So if anyone who kinds to share their protocol and experienced are greatly appreciated. For your information also, the Methacarn fixative mixture is (methanol:Chloroform:Glacial?Acetic Acid,60:30:10)?while the polyester wax is from Electron Microscopy Sciences deviced by Dr. H. F. Steedman. This tissue block will further use for Laser microdissection technique. Waiting for your quick response ASAP. ? Thank You in advance. ? Shazana Hilda Shamsuddin MSc.of Molecular Pathology, Dept. of Pathology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia. Off. no: +609- 766 4440 Fax no: +609- 765 3370 Email: hilda_1075@yahoo.com ? From Sue.Ford <@t> nhls.ac.za Tue Jul 29 00:40:24 2008 From: Sue.Ford <@t> nhls.ac.za (Sue Ford) Date: Tue Jul 29 00:33:20 2008 Subject: [Histonet] RE: Subscribe please Message-ID: <9D90CAC6872FD94FB3ADC25FFA7D34060441E4@nhlsctm002.NHLS.AC.ZA> -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: 29 July 2008 01:47 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 56, Issue 33 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Immuno Stainer (Sandra Cheasty) 2. Used embedding center (Va Paula Sicurello) 3. RE: Alcohol lamps (Bonner, Janet) 4. RE: Alcohol lamps (Thomas Jasper) 5. Blue Zones (Okland, Gloria A) 6. alcohol lamps (sharon.osborn@comcast.net) 7. Re: Alcohol lamps (Gayle Callis) 8. RE: Alcohol lamps (Monfils, Paul) 9. Re: alcohol lamps (Jackie M O'Connor) 10. Re: Scale for weighing parathyroid (Robert Richmond) 11. RE: Alcohol lamps (Rittman, Barry R) 12. RE: alcohol lamps (Smith, Allen) 13. RE: alcohol lamps (Jackie M O'Connor) 14. Human Herpes Virus 6 (Drew Sally A.) 15. Re: alcohol lamps (Victor Tobias) 16. Re: Human Herpes Virus 6 (tbritten@aol.com) 17. Creutz Jacob Disease Fitxtion, Hanling, and disposal of infected tissue (Robert Fauck [CCDHB]) 18. Sodium barbital buffer substitute (Barbara Albert) 19. RE: Alcohol lamps (Tony Henwood) ---------------------------------------------------------------------- Message: 1 Date: Mon, 28 Jul 2008 12:07:19 -0500 From: "Sandra Cheasty" Subject: [Histonet] Immuno Stainer To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Any suggestions re: an "open" Immunostainer that allows multiple detection kits, and also allows starting a new run before the previous run has completed? I work with veterinary diagnostic and research tissues. Thank you, Sandy UW-Madison ------------------------------ Message: 2 Date: Mon, 28 Jul 2008 10:44:45 -0700 (PDT) From: Va Paula Sicurello Subject: [Histonet] Used embedding center To: histonet@lists.utsouthwestern.edu Message-ID: <214245.32808.qm@web46112.mail.sp1.yahoo.com> Content-Type: text/plain; charset=us-ascii Hi HistoPeople, I'm looking for a used, but one that works, embedding center. I'm in the process of adding a histology service to my EM and confocal lab and have everything except the embedding center. If you have one laying around in a closet somewhere, I'll pay for shipping. Please contact me if you have one that you'd like to get rid of. Paula Sicurello VA San Diego Healthcare System Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 ------------------------------ Message: 3 Date: Mon, 28 Jul 2008 13:43:47 -0400 From: "Bonner, Janet" Subject: RE: [Histonet] Alcohol lamps To: "Peterson, Dan" <1dpeterson@meriter.com>, histonet@pathology.swmed.edu Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2726@fhosxchmb006.ADVENTISTCORP.NET> Content-Type: text/plain; charset=iso-8859-1 ...and what about those xylene fumes that us old-timers cannot detect with our burned out noses? A flame will ignite xylene as well. Otherwise, we could all go back to "smokin' in da lab"!! Janet L. Bonner, HTL (ASCP) Pathology Laboratory Florida Hospital Winter Park janet.bonner@FLHOSP.org 407-646-7559 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Peterson, Dan Sent: Mon 7/28/2008 12:21 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Alcohol lamps Fellow Histonetters, I am in disagreement with our lab's Safety officer with regards to alcohol lamps. We use them in our embedding area to keep our forceps clean. The officer says that they're a fire hazard (even though we've used them without incident for over 30 yrs). There are no flammable reagents (other than the alcohol in the lamp) near the embedding area. I know we could use the warming wells on the embedders, but try to find more that 1 pair of forceps that you like, or better yet, try to find a forceps that the tech before hasn't left paraffin all over it. (Yes, I am a fussy old goat, 27 yrs of Histo, with my 1 favorite pair that NOBODY touches) Petty issue? Are there others out there using lamps? I am willing to change if necessary (or so ordered), but would like to hear from those who do the work, not be told what to do by those who know nothing of the work. Thanks in advance!! Daniel R Peterson HT(ASCP) Histopathology Section Head Meriter Laboratories (608)-417-6557 1dpeterson@meriter.com CONFIDENTIALITY NOTICE: This message (including any attachments) is intended for the sole use of the individual and entity to whom it is addressed. This message may contain information that is confidential and is protected by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply email and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= ------------------------------ Message: 4 Date: Mon, 28 Jul 2008 11:05:51 -0700 From: "Thomas Jasper" Subject: RE: [Histonet] Alcohol lamps To: "Peterson, Dan" <1dpeterson@meriter.com> Cc: histonet@lists.utsouthwestern.edu Message-ID: <90354A475B420441B2A0396E5008D4965E211F@copc-sbs.COPC.local> Content-Type: text/plain; charset="US-ASCII" Hey Dan, Am compelled to respond as I know just what you mean about having a flame available. Back in the "good old days" about 10 years ago, at my previous employer, we were forced to get rid of our Bunsen burners. These of course provided a flame just like your alcohol lamps. I do have to say they may have been a bit more dangerous as they were fed by a tube of gas...I'm sure you get the picture. Anyway, not only do I agree with you about one forceps and the paraffin coating deal. I also liked the fact that the flame eliminated any residuals, between blocks. I always thought that was a GLP re: cross contamination, floaters, etc. However overall lab safety trumped my GLP reasons. And as you've stated...no accidents, ever to my knowledge, in my old lab. I have to admit, when we lost the burners I had a sad feeling. It may seem a bit silly, but I felt it really changed how efficiently and effectively we could get our embedding done. Eventually we obtained some bacti-incinerators (I think this has been mentioned already). They're ok, but you've really got to watch it as the forceps heat so quickly you'll burn your hand. It'd be nice if you could get something like a bacti-incinerator at a few thousand degrees cooler!? So Dan, not that my story helps your cause any, but at least you know I sympathize. I do have the feeling your safety officer is probably going to be able to throw his/her weight around with you on this one. Where I'm working now, we don't have bacti-incinerators. We only have the warming wells, and I try and rotate 2 or 3 of my "favorite" pairs of forceps when embedding. I know I'd be devastated if there'd ever been an accident with flammables (as anyone would). And I'm sure your safety officer will make the point about paraffin being flammable near your alcohol lamp. I believe safety officers are forced to look at things a certain way. There's no way he/she is ever going to understand where you're coming from and probably has a hard time understanding what your problem is. But it's not their job to get your job done...what to do? Think you'll have to suck it up and say good bye to your trusty lamps. Fellow former fan and friend of fire, Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, OR 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Peterson, Dan Sent: Monday, July 28, 2008 9:21 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Alcohol lamps Fellow Histonetters, I am in disagreement with our lab's Safety officer with regards to alcohol lamps. We use them in our embedding area to keep our forceps clean. The officer says that they're a fire hazard (even though we've used them without incident for over 30 yrs). There are no flammable reagents (other than the alcohol in the lamp) near the embedding area. I know we could use the warming wells on the embedders, but try to find more that 1 pair of forceps that you like, or better yet, try to find a forceps that the tech before hasn't left paraffin all over it. (Yes, I am a fussy old goat, 27 yrs of Histo, with my 1 favorite pair that NOBODY touches) Petty issue? Are there others out there using lamps? I am willing to change if necessary (or so ordered), but would like to hear from those who do the work, not be told what to do by those who know nothing of the work. Thanks in advance!! Daniel R Peterson HT(ASCP) Histopathology Section Head Meriter Laboratories (608)-417-6557 1dpeterson@meriter.com CONFIDENTIALITY NOTICE: This message (including any attachments) is intended for the sole use of the individual and entity to whom it is addressed. This message may contain information that is confidential and is protected by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply email and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Mon, 28 Jul 2008 14:20:34 -0400 From: "Okland, Gloria A" Subject: [Histonet] Blue Zones To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi All, We have been seeing a very random appearance of Blue Zones on our H&E slides. It affects one section, but not the next in serial sections and if we restain the slides, they look better, and it seems to just be the GI biopsies that we have trouble with. It seems like the tissue is not being stained by the eosin in these areas. The slides are deparraffinized and hydrated, and the hematoxylin stain looks ok, it is just the Eosin. Any suggestions that you may have, are greatly appreciated, this have been an ongoing problem for some time now. Thanks Gloria Gloria Okland HT (ASCP) Histology Supervisor Quest Diagnostics 4770 Regent Blvd Irving, TX 75063 972-536-8820 972-916-3539 fax ------------------------------------------ The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. ------------------------------ Message: 6 Date: Mon, 28 Jul 2008 18:29:51 +0000 From: sharon.osborn@comcast.net Subject: [Histonet] alcohol lamps To: histonet@pathology.swmed.edu Message-ID: <072820081829.13697.488E101F00063A1F000035812215593414029D010D9C01D202019D0E089C@comcast.net> Dan, About ten years ago, in setting up a new histology laboratory, management, techs, pathologists and I had this discussion. Safety regulations require no open flames in a laboratory area due to the explosion capabilities as well as the fire hazards. I had already told the techs we would not be using the alcohol lamps and we did not install gas lines in the new facility build out. This was already determined by the pathology group before the building commenced. There were 2-3 older techs (like me) who preferred to use the flames to clear the forceps of contamination from tissue and paraffin before embedding a new block. Yes, it is faster, however, very dangerous. i, too had done it for millenimum as you w/o risk. Management thought they would get on the good side of the techs and surprise them with alcohol burners. I discovered this before the techs came in and removed the alcohol lamps. Thank goodness, the head pathologist backed me up. As a post-doc he had a horrific personal experience in having an alcohol lamp ignite his lab coat when he accidentally brushed his sleeve across it. He understood the implications of the open flame in the lab. Also, where there are alcohols, xylenes or other similar flammable solvents around, the vapors could accidentally build up to create an easily combustible situation. So, the techs learned to use lots of Kim Wipes on the forceps before placing them in the warmers and to use Q-tips to k eep the warmers clear of tissue pieces that could contaminate a block. And, over time, it really does not slow you down that much in the embedding process. It is a change to not use the alcohol burners; however, the safety benefits far outweigh the hazards imposed by the open flame. And, as is often the case, the embedding person may be the only one in the laboratory EARLY in the morning and no one would know there was a fire until it might be too late. Sharon Osborn, BS, HT(ASCP) C Lab Vision Fremont, CA Date: Mon, 28 Jul 2008 11:21:28 -0500 From: "Peterson, Dan" <1dpeterson@meriter.com> Subject: [Histonet] Alcohol lamps To: Message-ID: <328CBAE62F31C642B422970E879DFADC01A80301@pcwex01> Content-Type: text/plain; charset="us-ascii" Fellow Histonetters, I am in disagreement with our lab's Safety officer with regards to alcohol lamps. We use them in our embedding area to keep our forceps clean. The officer says that they're a fire hazard (even though we've used them without incident for over 30 yrs). There are no flammable reagents (other than the alcohol in the lamp) near the embedding area. I know we could use the warming wells on the embedders, but try to find more that 1 pair of forceps that you like, or better yet, try to find a forceps that the tech before hasn't left paraffin all over it. (Yes, I am a fussy old goat, 27 yrs of Histo, with my 1 favorite pair that NOBODY touches) Petty issue? Are there others out there using lamps? I am willing to change if necessary (or so ordered), but would like to hear from those who do the work, not be told what to do by those who know nothing of the work. Thanks in advance!! Daniel R Peterson HT(ASCP) Histopathology Section Head Meriter Laboratories (608)-417-6557 1dpeterson@meriter.com ------------------------------ Message: 7 Date: Mon, 28 Jul 2008 12:48:33 -0600 From: "Gayle Callis" Subject: Re: [Histonet] Alcohol lamps To: "Peterson, Dan" <1dpeterson@meriter.com>, Message-ID: <001b01c8f0e2$89846710$6401a8c0@Sunney> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original We don't use open flames i.e. alcohol burners anymore, due to fire hazard (one could tip it over, spill alcohol and burn!). The forcep tips, if held too long in the flame, can also burn the tissue. We use heated embedding center wells but purchase longer forceps i.e. ergonomically acceptable You can buy forceps with L,hockey stick (packing forceps) shape to them, or buy straight with both wider and narrower, but NOT sharp pointed ends. We keep more one forceps to choose from in our wells, users can chose their favorites out this selection . We like longer, slender forceps for following reasons: 1. do not burn fingers during embedding 2. manipulate small tissues easily Try Arista Surgical Supply (www.aristasurgical.com , EMS, and Henry Schein (dentristy forceps, packing style) - none of these companies will break the bank and Arista has a HUGE selection of all types of forceps. As for paraffin on forceps, after embedding, lay them in cassette holding area on paper towel to soak up melted paraffin. Wipe them off while hot, return to wells when needed. In our lab, we have as many as 10 or more different people embedding their samples and THEY learn to do this very quickly. No moe complaining about paraffin all over the forceps this way. One can also try the metal mold holding area - where temp may be hot enought to melt the paraffin. We put forceps through cleaning cycle on processor and on occasion, a good paraffin removing solvent soak, rinsed off with alcohol or even a superhot water wash. If you have your favorite forceps, then others must have favorites too? Why not supply each person with their favorite too - then you are a hero instead of a "fussy old goat"? Gayle M. Callis HTL/HT/MT(ASCP) ----- Original Message ----- From: "Peterson, Dan" <1dpeterson@meriter.com> To: Sent: Monday, July 28, 2008 10:21 AM Subject: [Histonet] Alcohol lamps Fellow Histonetters, I am in disagreement with our lab's Safety officer with regards to alcohol lamps. We use them in our embedding area to keep our forceps clean. The officer says that they're a fire hazard (even though we've used them without incident for over 30 yrs). There are no flammable reagents (other than the alcohol in the lamp) near the embedding area. I know we could use the warming wells on the embedders, but try to find more that 1 pair of forceps that you like, or better yet, try to find a forceps that the tech before hasn't left paraffin all over it. (Yes, I am a fussy old goat, 27 yrs of Histo, with my 1 favorite pair that NOBODY touches) Petty issue? Are there others out there using lamps? I am willing to change if necessary (or so ordered), but would like to hear from those who do the work, not be told what to do by those who know nothing of the work. Thanks in advance!! Daniel R Peterson HT(ASCP) Histopathology Section Head Meriter Laboratories (608)-417-6557 1dpeterson@meriter.com CONFIDENTIALITY NOTICE: This message (including any attachments) is intended for the sole use of the individual and entity to whom it is addressed. This message may contain information that is confidential and is protected by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply email and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Mon, 28 Jul 2008 15:01:12 -0400 From: "Monfils, Paul" Subject: RE: [Histonet] Alcohol lamps To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835C40@LSRIEXCH1.lsmaster.lifespan.org> Content-Type: text/plain; charset="iso-8859-1" Another fussy old goat here! Alcohol lamps have served me well for 40 years, and I don't plan on changing unless they threaten to make me go back to using an autotechnicon and embedding with a pitcher of wax! Paul M. ------------------------------ Message: 9 Date: Mon, 28 Jul 2008 14:41:22 -0500 From: Jackie M O'Connor Subject: Re: [Histonet] alcohol lamps To: sharon.osborn@comcast.net Cc: histonet-bounces@lists.utsouthwestern.edu, histonet@pathology.swmed.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" In 1971 in Chicago, there was a fire in a histology lab in Chicago because of an alcohol lamp. The ignited lamp was knocked off the bench, fell to the floor, jar broke, all the spilled alcohol was ignited over a large area which went on to ignite another open container of another flammable on the floor (cleaning xylene, I think). A terrible fire resulted. Two techs were badly burned, since there was only one door to the lab, they had to run through the fire to get out (lab was on the 9th floor). I don't know who they were, or how they are now - but I was the technician who went to work there after the fire. Jackie O' sharon.osborn@comcast.net Sent by: histonet-bounces@lists.utsouthwestern.edu 07/28/2008 01:29 PM To histonet@pathology.swmed.edu cc Subject [Histonet] alcohol lamps Dan, About ten years ago, in setting up a new histology laboratory, management, techs, pathologists and I had this discussion. Safety regulations require no open flames in a laboratory area due to the explosion capabilities as well as the fire hazards. I had already told the techs we would not be using the alcohol lamps and we did not install gas lines in the new facility build out. This was already determined by the pathology group before the building commenced. There were 2-3 older techs (like me) who preferred to use the flames to clear the forceps of contamination from tissue and paraffin before embedding a new block. Yes, it is faster, however, very dangerous. i, too had done it for millenimum as you w/o risk. Management thought they would get on the good side of the techs and surprise them with alcohol burners. I discovered this before the techs came in and removed the alcohol lamps. Thank goodness, the head pathologist backed me up. As a post-doc he had a horrific personal experience in having an alcohol lamp ignite his lab coat when he accidentally brushed his sleeve across it. He understood the implications of the open flame in the lab. Also, where there are alcohols, xylenes or other similar flammable solvents around, the vapors could accidentally build up to create an easily combustible situation. So, the techs learned to use lots of Kim Wipes on the forceps before placing them in the warmers and to use Q-tips to k eep the warmers clear of tissue pieces that could contaminate a block. And, over time, it really does not slow you down that much in the embedding process. It is a change to not use the alcohol burners; however, the safety benefits far outweigh the hazards imposed by the open flame. And, as is often the case, the embedding person may be the only one in the laboratory EARLY in the morning and no one would know there was a fire until it might be too late. Sharon Osborn, BS, HT(ASCP) C Lab Vision Fremont, CA Date: Mon, 28 Jul 2008 11:21:28 -0500 From: "Peterson, Dan" <1dpeterson@meriter.com> Subject: [Histonet] Alcohol lamps To: Message-ID: <328CBAE62F31C642B422970E879DFADC01A80301@pcwex01> Content-Type: text/plain; charset="us-ascii" Fellow Histonetters, I am in disagreement with our lab's Safety officer with regards to alcohol lamps. We use them in our embedding area to keep our forceps clean. The officer says that they're a fire hazard (even though we've used them without incident for over 30 yrs). There are no flammable reagents (other than the alcohol in the lamp) near the embedding area. I know we could use the warming wells on the embedders, but try to find more that 1 pair of forceps that you like, or better yet, try to find a forceps that the tech before hasn't left paraffin all over it. (Yes, I am a fussy old goat, 27 yrs of Histo, with my 1 favorite pair that NOBODY touches) Petty issue? Are there others out there using lamps? I am willing to change if necessary (or so ordered), but would like to hear from those who do the work, not be told what to do by those who know nothing of the work. Thanks in advance!! Daniel R Peterson HT(ASCP) Histopathology Section Head Meriter Laboratories (608)-417-6557 1dpeterson@meriter.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Mon, 28 Jul 2008 15:43:50 -0400 From: "Robert Richmond" Subject: [Histonet] Re: Scale for weighing parathyroid To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Stacy McLaughlin HT (ASCP) at Cooley Dickinson Hospital in Northampton MA asks: >>My hospital will soon be starting parathyroid surgeries. Can anyone recommend a good ultra-sensitive scale for weighing them?<< I asked this question several days ago, here and on the pathology list, and got no responses. I've been researching the question today, and I've found a balance that MAY be suitable. What you need is a balance that will go up to about 100 grams, in increments of 10 milligrams. (Normal parathyroids run a few tens of milligrams, while adenomas can go well over a gram.) I called Fisher Scientific customer service at 800-766-7000. Their model S40152 (in the online catalog) has been replaced by their S94792B. These balances are made for Fisher by Ohaus. The Fisher representative said that the S94792B, also called the SLF 152, is a simplified version of Ohaus's Ohaus Valor 3000 Xtreme V31XH202, which costs about $400. Fisher sells the simpler model for $228. The Ohaus customer service person (at 800-526-0659, 2, 1) wasn't familiar with the balances Ohaus makes for Fisher. Before you go buying anything, check if your hospital pharmacy still has a balance. That's what I've always done with parathyroid adenomas - not very frequent specimens - but these days a lot of small hospital pharmacies no longer compound. Can anyone else on Histonet weigh in (sorry about that!) with some recommendations? Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 11 Date: Mon, 28 Jul 2008 14:49:14 -0500 From: "Rittman, Barry R" Subject: RE: [Histonet] Alcohol lamps To: "Peterson, Dan" <1dpeterson@meriter.com>, Message-ID: Content-Type: text/plain; charset="iso-8859-1" Like most things that are potentially dangerous, alcohol lamp may be a hazard. It is however the person using the lamp that is often the greatest hazard. I have seen a person stick them selves accidentally in the hand with a pencil. Should we then ban all pointed objects from the lab as they are potentially dangerous? I believe that if the individuals using the lamps are trained and adhere to appropriate safety procedures there is no problem. I do feel on the other hand, however that some of the alcohol lamp designs are not as stable as they should be. This can however be corrected by placing several small glass beads in the alcohol reservoir to make them less likely to tip over. Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Peterson, Dan Sent: Mon 7/28/2008 11:21 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Alcohol lamps Fellow Histonetters, I am in disagreement with our lab's Safety officer with regards to alcohol lamps. We use them in our embedding area to keep our forceps clean. The officer says that they're a fire hazard (even though we've used them without incident for over 30 yrs). There are no flammable reagents (other than the alcohol in the lamp) near the embedding area. I know we could use the warming wells on the embedders, but try to find more that 1 pair of forceps that you like, or better yet, try to find a forceps that the tech before hasn't left paraffin all over it. (Yes, I am a fussy old goat, 27 yrs of Histo, with my 1 favorite pair that NOBODY touches) Petty issue? Are there others out there using lamps? I am willing to change if necessary (or so ordered), but would like to hear from those who do the work, not be told what to do by those who know nothing of the work. Thanks in advance!! Daniel R Peterson HT(ASCP) Histopathology Section Head Meriter Laboratories (608)-417-6557 1dpeterson@meriter.com CONFIDENTIALITY NOTICE: This message (including any attachments) is intended for the sole use of the individual and entity to whom it is addressed. This message may contain information that is confidential and is protected by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply email and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Mon, 28 Jul 2008 16:25:24 -0400 From: "Smith, Allen" Subject: RE: [Histonet] alcohol lamps To: 'Jackie M O'Connor' Cc: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" I have worried about this possibility. This is why I use a stamped steel alcohol lamp. The larger fire was the result of poor communication. Before lighting, I announce: I am about to strike a match. All solvents must be covered! (By the way, where was that lab's fire extinguisher?) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Monday, July 28, 2008 3:41 PM To: sharon.osborn@comcast.net Cc: histonet-bounces@lists.utsouthwestern.edu; histonet@pathology.swmed.edu Subject: Re: [Histonet] alcohol lamps In 1971 in Chicago, there was a fire in a histology lab in Chicago because of an alcohol lamp. The ignited lamp was knocked off the bench, fell to the floor, jar broke, all the spilled alcohol was ignited over a large area which went on to ignite another open container of another flammable on the floor (cleaning xylene, I think). A terrible fire resulted. Two techs were badly burned, since there was only one door to the lab, they had to run through the fire to get out (lab was on the 9th floor). I don't know who they were, or how they are now - but I was the technician who went to work there after the fire. Jackie O' sharon.osborn@comcast.net Sent by: histonet-bounces@lists.utsouthwestern.edu 07/28/2008 01:29 PM To histonet@pathology.swmed.edu cc Subject [Histonet] alcohol lamps Dan, About ten years ago, in setting up a new histology laboratory, management, techs, pathologists and I had this discussion. Safety regulations require no open flames in a laboratory area due to the explosion capabilities as well as the fire hazards. I had already told the techs we would not be using the alcohol lamps and we did not install gas lines in the new facility build out. This was already determined by the pathology group before the building commenced. There were 2-3 older techs (like me) who preferred to use the flames to clear the forceps of contamination from tissue and paraffin before embedding a new block. Yes, it is faster, however, very dangerous. i, too had done it for millenimum as you w/o risk. Management thought they would get on the good side of the techs and surprise them with alcohol burners. I discovered this before the techs came in and removed the alcohol lamps. Thank goodness, the head pathologist backed me up. As a post-doc he had a horrific personal experience in having an alcohol lamp ignite his lab coat when he accidentally brushed his sleeve across it. He understood the implications of the open flame in the lab. Also, where there are alcohols, xylenes or other similar flammable solvents around, the vapors could accidentally build up to create an easily combustible situation. So, the techs learned to use lots of Kim Wipes on the forceps before placing them in the warmers and to use Q-tips to k eep the warmers clear of tissue pieces that could contaminate a block. And, over time, it really does not slow you down that much in the embedding process. It is a change to not use the alcohol burners; however, the safety benefits far outweigh the hazards imposed by the open flame. And, as is often the case, the embedding person may be the only one in the laboratory EARLY in the morning and no one would know there was a fire until it might be too late. Sharon Osborn, BS, HT(ASCP) C Lab Vision Fremont, CA Date: Mon, 28 Jul 2008 11:21:28 -0500 From: "Peterson, Dan" <1dpeterson@meriter.com> Subject: [Histonet] Alcohol lamps To: Message-ID: <328CBAE62F31C642B422970E879DFADC01A80301@pcwex01> Content-Type: text/plain; charset="us-ascii" Fellow Histonetters, I am in disagreement with our lab's Safety officer with regards to alcohol lamps. We use them in our embedding area to keep our forceps clean. The officer says that they're a fire hazard (even though we've used them without incident for over 30 yrs). There are no flammable reagents (other than the alcohol in the lamp) near the embedding area. I know we could use the warming wells on the embedders, but try to find more that 1 pair of forceps that you like, or better yet, try to find a forceps that the tech before hasn't left paraffin all over it. (Yes, I am a fussy old goat, 27 yrs of Histo, with my 1 favorite pair that NOBODY touches) Petty issue? Are there others out there using lamps? I am willing to change if necessary (or so ordered), but would like to hear from those who do the work, not be told what to do by those who know nothing of the work. Thanks in advance!! Daniel R Peterson HT(ASCP) Histopathology Section Head Meriter Laboratories (608)-417-6557 1dpeterson@meriter.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Mon, 28 Jul 2008 15:30:42 -0500 From: Jackie M O'Connor Subject: RE: [Histonet] alcohol lamps To: "Smith, Allen" Cc: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="US-ASCII" Probably outside the door in the hall. Remember, it was 1971 - - we could smoke and eat in the labs then, there were no safety rules. Heck, this lab only had one door. By the time I went to work there, they had made a second egress. "Smith, Allen" 07/28/2008 03:25 PM To 'Jackie M O'Connor' cc "'histonet@lists.utsouthwestern.edu'" Subject RE: [Histonet] alcohol lamps I have worried about this possibility. This is why I use a stamped steel alcohol lamp. The larger fire was the result of poor communication. Before lighting, I announce: I am about to strike a match. All solvents must be covered! (By the way, where was that lab's fire extinguisher?) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Monday, July 28, 2008 3:41 PM To: sharon.osborn@comcast.net Cc: histonet-bounces@lists.utsouthwestern.edu; histonet@pathology.swmed.edu Subject: Re: [Histonet] alcohol lamps In 1971 in Chicago, there was a fire in a histology lab in Chicago because of an alcohol lamp. The ignited lamp was knocked off the bench, fell to the floor, jar broke, all the spilled alcohol was ignited over a large area which went on to ignite another open container of another flammable on the floor (cleaning xylene, I think). A terrible fire resulted. Two techs were badly burned, since there was only one door to the lab, they had to run through the fire to get out (lab was on the 9th floor). I don't know who they were, or how they are now - but I was the technician who went to work there after the fire. Jackie O' sharon.osborn@comcast.net Sent by: histonet-bounces@lists.utsouthwestern.edu 07/28/2008 01:29 PM To histonet@pathology.swmed.edu cc Subject [Histonet] alcohol lamps Dan, About ten years ago, in setting up a new histology laboratory, management, techs, pathologists and I had this discussion. Safety regulations require no open flames in a laboratory area due to the explosion capabilities as well as the fire hazards. I had already told the techs we would not be using the alcohol lamps and we did not install gas lines in the new facility build out. This was already determined by the pathology group before the building commenced. There were 2-3 older techs (like me) who preferred to use the flames to clear the forceps of contamination from tissue and paraffin before embedding a new block. Yes, it is faster, however, very dangerous. i, too had done it for millenimum as you w/o risk. Management thought they would get on the good side of the techs and surprise them with alcohol burners. I discovered this before the techs came in and removed the alcohol lamps. Thank goodness, the head pathologist backed me up. As a post-doc he had a horrific personal experience in having an alcohol lamp ignite his lab coat when he accidentally brushed his sleeve across it. He understood the implications of the open flame in the lab. Also, where there are alcohols, xylenes or other similar flammable solvents around, the vapors could accidentally build up to create an easily combustible situation. So, the techs learned to use lots of Kim Wipes on the forceps before placing them in the warmers and to use Q-tips to k eep the warmers clear of tissue pieces that could contaminate a block. And, over time, it really does not slow you down that much in the embedding process. It is a change to not use the alcohol burners; however, the safety benefits far outweigh the hazards imposed by the open flame. And, as is often the case, the embedding person may be the only one in the laboratory EARLY in the morning and no one would know there was a fire until it might be too late. Sharon Osborn, BS, HT(ASCP) C Lab Vision Fremont, CA Date: Mon, 28 Jul 2008 11:21:28 -0500 From: "Peterson, Dan" <1dpeterson@meriter.com> Subject: [Histonet] Alcohol lamps To: Message-ID: <328CBAE62F31C642B422970E879DFADC01A80301@pcwex01> Content-Type: text/plain; charset="us-ascii" Fellow Histonetters, I am in disagreement with our lab's Safety officer with regards to alcohol lamps. We use them in our embedding area to keep our forceps clean. The officer says that they're a fire hazard (even though we've used them without incident for over 30 yrs). There are no flammable reagents (other than the alcohol in the lamp) near the embedding area. I know we could use the warming wells on the embedders, but try to find more that 1 pair of forceps that you like, or better yet, try to find a forceps that the tech before hasn't left paraffin all over it. (Yes, I am a fussy old goat, 27 yrs of Histo, with my 1 favorite pair that NOBODY touches) Petty issue? Are there others out there using lamps? I am willing to change if necessary (or so ordered), but would like to hear from those who do the work, not be told what to do by those who know nothing of the work. Thanks in advance!! Daniel R Peterson HT(ASCP) Histopathology Section Head Meriter Laboratories (608)-417-6557 1dpeterson@meriter.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Mon, 28 Jul 2008 15:34:31 -0500 From: "Drew Sally A." Subject: [Histonet] Human Herpes Virus 6 To: "Histonet" Message-ID: <3F328377AF4E23438E78234752652CE105D5276A@uwhis-xchng7.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="US-ASCII" I've been asked to find out if there are any reference labs "out there" that offer HHV-6? Thank you for any leads offered! Sally Ann Drew, MT (ASCP) sdrew@uwhealth.org IHC/ISH Lab DB1-223, Mail Code 3224 600 Highland Ave. Madison, WI 53792 Phone (608) 265-6596 Fax (608) 262-7174 ------------------------------ Message: 15 Date: Mon, 28 Jul 2008 13:43:28 -0700 From: Victor Tobias Subject: Re: [Histonet] alcohol lamps To: "Jackie M O'Connor" Cc: histonet-bounces@lists.utsouthwestern.edu, sharon.osborn@comcast.net, histonet@pathology.swmed.edu Message-ID: <488E2F70.5050103@pathology.washington.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed What a coincidence that it happened 100 years later in Chicago, can't blame it on the cow this time. http://www.thechicagofire.com/ Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Jackie M O'Connor wrote: > In 1971 in Chicago, there was a fire in a histology lab in Chicago because > of an alcohol lamp. The ignited lamp was knocked off the bench, fell to > the floor, jar broke, all the spilled alcohol was ignited over a large > area which went on to ignite another open container of another flammable > on the floor (cleaning xylene, I think). A terrible fire resulted. Two > techs were badly burned, since there was only one door to the lab, they > had to run through the fire to get out (lab was on the 9th floor). I > don't know who they were, or how they are now - but I was the technician > who went to work there after the fire. > Jackie O' > > > > > sharon.osborn@comcast.net > Sent by: histonet-bounces@lists.utsouthwestern.edu > 07/28/2008 01:29 PM > > To > histonet@pathology.swmed.edu > cc > > Subject > [Histonet] alcohol lamps > > > > > > > > Dan, > About ten years ago, in setting up a new histology laboratory, > management, techs, pathologists and I had this discussion. Safety > regulations require no open flames in a laboratory area due to the > explosion capabilities as well as the fire hazards. I had already told > the techs we would not be using the alcohol lamps and we did not install > gas lines in the new facility build out. This was already determined by > the pathology group before the building commenced. > There were 2-3 older techs (like me) who preferred to use the flames to > clear the forceps of contamination from tissue and paraffin before > embedding a new block. Yes, it is faster, however, very dangerous. i, > too had done it for millenimum as you w/o risk. Management thought they > would get on the good side of the techs and surprise them with alcohol > burners. I discovered this before the techs came in and removed the > alcohol lamps. Thank goodness, the head pathologist backed me up. As a > post-doc he had a horrific personal experience in having an alcohol lamp > ignite his lab coat when he accidentally brushed his sleeve across it. He > understood the implications of the open flame in the lab. Also, where > there are alcohols, xylenes or other similar flammable solvents around, > the vapors could accidentally build up to create an easily combustible > situation. So, the techs learned to use lots of Kim Wipes on the forceps > before placing them in the warmers and to use Q-tips to k > eep the warmers clear of tissue pieces that could contaminate a block. > And, over time, it really does not slow you down that much in the > embedding process. > It is a change to not use the alcohol burners; however, the safety > benefits far outweigh the hazards imposed by the open flame. And, as is > often the case, the embedding person may be the only one in the laboratory > EARLY in the morning and no one would know there was a fire until it might > be too late. > > Sharon Osborn, BS, HT(ASCP) C > Lab Vision > Fremont, CA > > > > Date: Mon, 28 Jul 2008 11:21:28 -0500 > From: "Peterson, Dan" <1dpeterson@meriter.com> > Subject: [Histonet] Alcohol lamps > To: > Message-ID: <328CBAE62F31C642B422970E879DFADC01A80301@pcwex01> > Content-Type: text/plain; charset="us-ascii" > > Fellow Histonetters, > I am in disagreement with our lab's Safety officer with regards to > alcohol lamps. We use them in our embedding area to keep our forceps > clean. > The officer says that they're a fire hazard (even though we've used them > without incident for over 30 yrs). There are no flammable reagents > (other than the alcohol in the lamp) near the embedding area. I know we > could use the warming wells on the embedders, but try to find more that > 1 pair of forceps that you like, or better yet, try to find a forceps > that the tech before hasn't left paraffin all over it. (Yes, I am a > fussy old goat, 27 yrs of Histo, with my 1 favorite pair that NOBODY > touches) > Petty issue? Are there others out there using lamps? I am willing to > change if necessary (or so ordered), but would like to hear from those > who do the work, not be told what to do by those who know nothing of the > work. Thanks in advance!! > > Daniel R Peterson HT(ASCP) > Histopathology Section Head > Meriter Laboratories > (608)-417-6557 > 1dpeterson@meriter.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 16 Date: Mon, 28 Jul 2008 20:43:46 +0000 From: tbritten@aol.com Subject: Re: [Histonet] Human Herpes Virus 6 To: "Drew Sally A." , histonet-bounces@lists.utsouthwestern.edu, "Histonet" Message-ID: <1492695284-1217277835-cardhu_decombobulator_blackberry.rim.net-176270595-@bxe197.bisx.prod.on.blackberry> Content-Type: text/plain Hi sally; we offer the slides if that helps. I don't want this to be a commercial for us. Would you like to contact me off line? Regards tom britten ------Original Message------ From: Drew Sally A. Sender: histonet-bounces@lists.utsouthwestern.edu To: Histonet Sent: Jul 28, 2008 4:34 PM Subject: [Histonet] Human Herpes Virus 6 I've been asked to find out if there are any reference labs "out there" that offer HHV-6? Thank you for any leads offered! Sally Ann Drew, MT (ASCP) sdrew@uwhealth.org IHC/ISH Lab DB1-223, Mail Code 3224 600 Highland Ave. Madison, WI 53792 Phone (608) 265-6596 Fax (608) 262-7174 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sent from my Verizon Wireless BlackBerry ------------------------------ Message: 17 Date: Tue, 29 Jul 2008 10:55:35 +1200 From: "Robert Fauck [CCDHB]" Subject: [Histonet] Creutz Jacob Disease Fitxtion, Hanling, and disposal of infected tissue To: histonet@lists.utsouthwestern.edu Message-ID: <5587F098B7C4EE489C8BE204E9565548248D12@wn0nteml05.hiq.net.nz> Content-Type: text/plain; charset=us-ascii Hi Everybody Can somebody give me an update on CJD fixation, handling and disposal of CJD infected ( or suspected) tissue, please. Thanking you in advance for your expertise and help. Regards, Robert Fauck Wellington Hospital, New Zealand This email or attachment(s) may contain confidential or legally privileged information intended for the sole use of the addressee(s). Any use, redistribution, disclosure, or reproduction of this message, except as intended, is prohibited. If you received this email in error, please notify the sender and remove all copies of the message, including any attachments. Any views or opinions expressed in this email (unless otherwise stated) may not represent those of Capital & Coast District Health Board. http://www.ccdhb.org.nz (1C_S1) No Viruses were detected in this message. HealthIntelligence eMail Filter Service ------------------------------ Message: 18 Date: Mon, 28 Jul 2008 16:08:46 -0700 (PDT) From: Barbara Albert Subject: [Histonet] Sodium barbital buffer substitute To: histonet@lists.utsouthwestern.edu Message-ID: <356339.21004.qm@web63707.mail.re1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi all, I've been asked to post this question to the list.? What do you use as a substitute for Sodium barbitol buffer in your muscle histochemistries?? ? Thanks, Barbara Albert UCSF Medical Center San Francisco ------------------------------ Message: 19 Date: Tue, 29 Jul 2008 09:32:47 +1000 From: "Tony Henwood" Subject: RE: [Histonet] Alcohol lamps To: "Peterson, Dan" <1dpeterson@meriter.com>, Message-ID: Content-Type: text/plain; charset="us-ascii" There have been incidents of fires at embedding benches, the wax can ignite (i.e. wax candles). Also alcohol fires are invisible (refer to your famous NASCARs). There have been at least 4 that I am aware of in Australia. It is not something that would be well publicised since it is the embarrassment that tends to keep it quiet! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Peterson, Dan Sent: Tuesday, 29 July 2008 2:21 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Alcohol lamps Fellow Histonetters, I am in disagreement with our lab's Safety officer with regards to alcohol lamps. We use them in our embedding area to keep our forceps clean. The officer says that they're a fire hazard (even though we've used them without incident for over 30 yrs). There are no flammable reagents (other than the alcohol in the lamp) near the embedding area. I know we could use the warming wells on the embedders, but try to find more that 1 pair of forceps that you like, or better yet, try to find a forceps that the tech before hasn't left paraffin all over it. (Yes, I am a fussy old goat, 27 yrs of Histo, with my 1 favorite pair that NOBODY touches) Petty issue? Are there others out there using lamps? I am willing to change if necessary (or so ordered), but would like to hear from those who do the work, not be told what to do by those who know nothing of the work. Thanks in advance!! Daniel R Peterson HT(ASCP) Histopathology Section Head Meriter Laboratories (608)-417-6557 1dpeterson@meriter.com CONFIDENTIALITY NOTICE: This message (including any attachments) is intended for the sole use of the individual and entity to whom it is addressed. This message may contain information that is confidential and is protected by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply email and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 56, Issue 33 **************************************** ********************************************************************************** The views expressed in this email are, unless otherwise stated, those of the author and not those of the National Health Laboratory Services or its management. The information in this e-mail is confidential and is intended solely for the addressee. Access to this e-mail by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken or omitted in reliance on this, is prohibited and may be unlawful. Whilst all reasonable steps are taken to ensure the accuracy and integrity of information and data transmitted electronically and to preserve the confidentiality thereof, no liability or responsibility whatsoever is accepted if information or data is, for whatever reason, corrupted or does not reach its intended destination. *********************************************************************************** From cmmathis1 <@t> bellsouth.net Tue Jul 29 09:39:22 2008 From: cmmathis1 <@t> bellsouth.net (cmmathis1@bellsouth.net) Date: Tue Jul 29 09:39:28 2008 Subject: [Histonet] Agarose embedding Message-ID: <072920081439.24216.488F2B9A0002748600005E9822243429029B0A02D2089B9A019C04040A0DBFCE9C07089B0E03030C@att.net> I need to embed some thin tissue membranes in agarose. Sigma lists 37 kinds. Any suggestions as to which type to use. Our protocol says to prepare a 2% solution and we tried this with an old bottle of agarose from Gibco that we found in the lab. When I processed the block, it shrank a lot. Any help would be greatly appreciated! Cathy From Kurtz.Mathew <@t> mayo.edu Tue Jul 29 09:58:37 2008 From: Kurtz.Mathew <@t> mayo.edu (Kurtz, Mathew) Date: Tue Jul 29 09:58:47 2008 Subject: [Histonet] Alcohol lamps References: <328CBAE62F31C642B422970E879DFADC01A80301@pcwex01> Message-ID: <0B8BA0705C5073449141EB5F44CF957001CA562E@LMMAILVS1.ad.lmmhs.org> Barry, I agree with you to a certain extent. Actually, you remind me of the debate whether or not to let people carry concealed weapons. Even highly trained people are still able to have an ACCIDENT(S). You will never know when or where it will happen, what you do know is it will happen. The proof is the in the story about the fire in the Chicago lab in 1971. The point is, if you get rid of them, it is one less accident waiting to happen. Although it may be a pain the butt, and we don't all agree, that is the goal of the safety Nazi....right!? Mathew P. Kurtz HT P.S. Hey Jasper!!! ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rittman, Barry R Sent: Mon 7/28/2008 2:49 PM To: Peterson, Dan; histonet@pathology.swmed.edu Subject: RE: [Histonet] Alcohol lamps Like most things that are potentially dangerous, alcohol lamp may be a hazard. It is however the person using the lamp that is often the greatest hazard. I have seen a person stick them selves accidentally in the hand with a pencil. Should we then ban all pointed objects from the lab as they are potentially dangerous? I believe that if the individuals using the lamps are trained and adhere to appropriate safety procedures there is no problem. I do feel on the other hand, however that some of the alcohol lamp designs are not as stable as they should be. This can however be corrected by placing several small glass beads in the alcohol reservoir to make them less likely to tip over. Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Peterson, Dan Sent: Mon 7/28/2008 11:21 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Alcohol lamps Fellow Histonetters, I am in disagreement with our lab's Safety officer with regards to alcohol lamps. We use them in our embedding area to keep our forceps clean. The officer says that they're a fire hazard (even though we've used them without incident for over 30 yrs). There are no flammable reagents (other than the alcohol in the lamp) near the embedding area. I know we could use the warming wells on the embedders, but try to find more that 1 pair of forceps that you like, or better yet, try to find a forceps that the tech before hasn't left paraffin all over it. (Yes, I am a fussy old goat, 27 yrs of Histo, with my 1 favorite pair that NOBODY touches) Petty issue? Are there others out there using lamps? I am willing to change if necessary (or so ordered), but would like to hear from those who do the work, not be told what to do by those who know nothing of the work. Thanks in advance!! Daniel R Peterson HT(ASCP) Histopathology Section Head Meriter Laboratories (608)-417-6557 1dpeterson@meriter.com CONFIDENTIALITY NOTICE: This message (including any attachments) is intended for the sole use of the individual and entity to whom it is addressed. This message may contain information that is confidential and is protected by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply email and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. From mpence <@t> grhs.net Tue Jul 29 10:29:00 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Jul 29 10:29:06 2008 Subject: [Histonet] Alcohol lamps In-Reply-To: <0B8BA0705C5073449141EB5F44CF957001CA562E@LMMAILVS1.ad.lmmhs.org> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A38AE@IS-E2K3.grhs.net> As the "safety Nazi" of our lab I wrote a policy that stated that there was to be no eating, drinking, or smoking in the lab. I also put in this policy that there was to be no open flames. This is best practice for safety and a condition of employment in our lab. Even if you are a "old timer" in the lab, times change and so does practices. You have to change with the times or get out of the lab business! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kurtz, Mathew Sent: Tuesday, July 29, 2008 9:59 AM To: Rittman, Barry R; Peterson, Dan; histonet@pathology.swmed.edu Subject: RE: [Histonet] Alcohol lamps Barry, I agree with you to a certain extent. Actually, you remind me of the debate whether or not to let people carry concealed weapons. Even highly trained people are still able to have an ACCIDENT(S). You will never know when or where it will happen, what you do know is it will happen. The proof is the in the story about the fire in the Chicago lab in 1971. The point is, if you get rid of them, it is one less accident waiting to happen. Although it may be a pain the butt, and we don't all agree, that is the goal of the safety Nazi....right!? Mathew P. Kurtz HT P.S. Hey Jasper!!! ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rittman, Barry R Sent: Mon 7/28/2008 2:49 PM To: Peterson, Dan; histonet@pathology.swmed.edu Subject: RE: [Histonet] Alcohol lamps Like most things that are potentially dangerous, alcohol lamp may be a hazard. It is however the person using the lamp that is often the greatest hazard. I have seen a person stick them selves accidentally in the hand with a pencil. Should we then ban all pointed objects from the lab as they are potentially dangerous? I believe that if the individuals using the lamps are trained and adhere to appropriate safety procedures there is no problem. I do feel on the other hand, however that some of the alcohol lamp designs are not as stable as they should be. This can however be corrected by placing several small glass beads in the alcohol reservoir to make them less likely to tip over. Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Peterson, Dan Sent: Mon 7/28/2008 11:21 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Alcohol lamps Fellow Histonetters, I am in disagreement with our lab's Safety officer with regards to alcohol lamps. We use them in our embedding area to keep our forceps clean. The officer says that they're a fire hazard (even though we've used them without incident for over 30 yrs). There are no flammable reagents (other than the alcohol in the lamp) near the embedding area. I know we could use the warming wells on the embedders, but try to find more that 1 pair of forceps that you like, or better yet, try to find a forceps that the tech before hasn't left paraffin all over it. (Yes, I am a fussy old goat, 27 yrs of Histo, with my 1 favorite pair that NOBODY touches) Petty issue? Are there others out there using lamps? I am willing to change if necessary (or so ordered), but would like to hear from those who do the work, not be told what to do by those who know nothing of the work. Thanks in advance!! Daniel R Peterson HT(ASCP) Histopathology Section Head Meriter Laboratories (608)-417-6557 1dpeterson@meriter.com CONFIDENTIALITY NOTICE: This message (including any attachments) is intended for the sole use of the individual and entity to whom it is addressed. This message may contain information that is confidential and is protected by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply email and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Tue Jul 29 11:33:33 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Tue Jul 29 11:33:38 2008 Subject: [Histonet] Leica Cassette Printer Message-ID: <57BE698966D5C54EAE8612E8941D7683036DD2B8@EXCHANGE3.huntingtonhospital.com> Is there anyone in the Los Angeles/Pasadena, CA area that has the Leica cassette printer? I would like to talk to you offline about a possible onsite visit. Laurie Colbert Huntington Hospital Pasadena, CA (626) 397-8620 From kenneth.a.troutman <@t> Vanderbilt.Edu Tue Jul 29 12:30:49 2008 From: kenneth.a.troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Tue Jul 29 12:30:56 2008 Subject: [Histonet] Immuno Stainer Message-ID: <37DEF9AF72994947AF693956A59B9B660127FF4A@mailbe03.mc.vanderbilt.edu> Hi Sandy, We use the Leica BondMax. It is the only stainer that I know of that will allow you to start a run and then load more slides while the others are running. (Twice, actually.) As for the multiple detection kits, I believe with the model we have, you have to use at least one of their reagents on their detection kit to run the protocols. (You can just use the hematoxylin if you don't want to use anything else.) There is also, I believe a research version of this instrument that may be more flexible. Hope this helps. Ashley Troutman BS, HT(ASCP)QIHC Histopathology Laboratory Department of Pathology Vanderbilt University Medical Center Nashville, TN Message: 1 Date: Mon, 28 Jul 2008 12:07:19 -0500 From: "Sandra Cheasty" Subject: [Histonet] Immuno Stainer To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Any suggestions re: an "open" Immunostainer that allows multiple detection kits, and also allows starting a new run before the previous run has completed? I work with veterinary diagnostic and research tissues. Thank you, Sandy UW-Madison From amber.mckenzie <@t> gastrodocs.net Tue Jul 29 13:23:55 2008 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Tue Jul 29 13:24:02 2008 Subject: [Histonet] cut control slides Message-ID: <03C921A1EAF7F541B16543F6EC6A4B3701D3A93D@giamail2.Gia.com> How long do you think cut control slides will last? From rjbuesa <@t> yahoo.com Tue Jul 29 13:33:32 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jul 29 13:33:36 2008 Subject: [Histonet] cut control slides In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B3701D3A93D@giamail2.Gia.com> Message-ID: <88508.963.qm@web65702.mail.ac4.yahoo.com> It will depend what controls you are referring to. "Routine" histochemistry controls last months if not years, BUT IHC controls will suffer from epitope oxidation sometimes in 1 or 2 weeks or less. Ren? J. --- On Tue, 7/29/08, Amber McKenzie wrote: From: Amber McKenzie Subject: [Histonet] cut control slides To: histonet@lists.utsouthwestern.edu Date: Tuesday, July 29, 2008, 2:23 PM How long do you think cut control slides will last? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dmikita <@t> wmcnet.org Tue Jul 29 13:53:22 2008 From: dmikita <@t> wmcnet.org (Daryl Mikita) Date: Tue Jul 29 13:54:03 2008 Subject: [Histonet] HT job opening Message-ID: <488F12C20200003D00009640@gwgate.wmcnet.org> Hello, Thought that I would post our new job opening on here. This is what is listed on our web page. If you have anymore questions please feel free to call (307) 577-2198. Daryl Mikita, HT(ASCP)cm Histologic Technician Job Code: 519 Posted: Jul-25-2008 - Department: Lab - Full-time - Day shift One year certificate from college or technical school; and three to six months related experience and/or training; or equivalent combination of education and experience. ASCP registered, equivalent or eligible Contact: Brandi Hammond Tel: 307.577.7996 Email: BHammond@wmcnet.org Address: Wyoming Medical Center 1233 E 2nd Street Casper, WY 82601 Daryl A. Mikita, HT(ASCP)cm Wyoming Medical Center Anatomical Pathology 1233 E. 2nd St. Casper, WY 82601 (307) 577-2198 From Bonnie.Whitaker <@t> osumc.edu Tue Jul 29 14:12:02 2008 From: Bonnie.Whitaker <@t> osumc.edu (Whitaker, Bonnie) Date: Tue Jul 29 14:12:23 2008 Subject: [Histonet] Histotech position in Columbus, OH Message-ID: <3CE20ED86C4A114EBDF3BCE8DEFD8F6026259E@msxc06.OSUMC.EDU> Hi Everyone, I have a position open for a histotech, minimum HT(ASCP) or eligible. This is a great place to live and work! Send me an email or phone me for details if you are interested!! HR asked me to include this statement in my posting: "The Ohio State University is an equal opportunity, affirmative active employer. Women, minorities, veterans, and individuals with disabilities are encouraged to apply." Thanks!! Bonnie Whitaker Clinical Histology Manager Ohio State University Medical Center N308B Doan Hall 410 W. 10th Ave. Columbus, OH 43210 Bonnie.Whitaker@osumc.edu phone 614.293.5048 fax 614.293.7273 From Jason.Burrill <@t> crl.com Tue Jul 29 14:37:58 2008 From: Jason.Burrill <@t> crl.com (Burrill, Jason) Date: Tue Jul 29 14:38:24 2008 Subject: [Histonet] Senior Histology Position Available in the Boston Area Message-ID: <1AD4E907E9B6F648AEF1B3A20A9B0E1E0140BAC7@shr-exch2.na01.crl.com> There is an exciting new opportunity opening in the Charles River Research Animal Diagnostic Lab located in Wilmington, MA. This position will be responsible for the technical direction of the Histology laboratory, developing new techniques and training technologists. I have included the job description below for those that are interested. Please note that this position does not include compensation or reimbursement for relocation. For more information about Charles River and this position you can visit www.criver.com and click on careers. To apply for this position please forward a resume and cover letter to james.garofalo@crl.com. Job Title: Research Assistant, Histology Job ID: 81053 Location: USA - MA - Wilmington Full/Part Time: Full-Time Regular/Temporary: Regular Responsibilities *Adhere to Good Laboratory Practice documentation policies while performing all histology lab procedures. *Perform all basic and advance histology processing steps as requested. *Revise and develop histology department Standard Operating Procedures (SOPs) and forms. *Evaluate the histopathology portions of protocols, and coordinate the conduct of health monitoring and study-associated pathology/histology work with the study director, health monitoring department or other diagnostic laboratory management, professional staff or Sponsors in order to provide consistent turnaround time, quality of finished product and appropriate scheduling of laboratory operations. *Serve as point of contact and provide technical guidance for all inquiries concerning external pathology submissions and histology laboratory capabilities. *Function as a technical liaison between pathologists and histology department staff. *Track and bill special study histopathology submissions and review health monitoring diagnostic billing. *Coordinate shipment of pathology reports and specimens to sponsor. *Review yearly workload and commercial billing and present to management. *Provide technical histology training and study sessions to histology staff. *Review outstanding histology services as they relate to pathology submissions. *Adhere to all departmental SOPs, policies, safety procedures and protocols. *Facilitate personnel and area monitoring of xylene and formaldehyde (recordkeeping). *Coordinate preventative maintenance of histology equipment. *Package, label and ship pathology specimens. *Develop, implement and train staff as appropriate on new and advanced procedures. Pair with management and professional staff to identify areas for development. *Contribute to the development and implementation of routine training for new employees. Provide feedback to management on training goals and progress. *Work productively in group situations (as a member and/or leader) as well as independently. *Provide leadership and motivation to departmental personnel. *Handle and dispose of hazardous chemicals and biological material according to laboratory safety policy. *Use document control system for updating and implementing controlled documents. *Accession and entry of data regarding specimens using ILIMS system. *Perform all other related duties as assigned. Qualifications *Education: Associate's degree (A.A./A.S.) in biological science required. Bachelor's degree (B.A./B.S.) preferred. *Experience: 4 to 6 years. *Certification/Licensure: HT or HTL (ASCP). Equal Employment Opportunity Charles River Laboratories is an equal opportunity employer who values diversity in the workplace. Jason Burrill Sr. Manager, Histology and Laboratory Safety Research Animal Diagnostic Services Charles River 251 Ballardvale St Wilmington, MA 01887 Office: 781-222-6152 Fax: 978-988-8793 jason.burrill@crl.com www.criver.com Accelerating Drug Development. Exactly. Notice - This email and any files transmitted with it are confidential and may contain privileged and/or proprietary information. You must not disclose this message to another party without Charles River's express written consent. If you are not the intended recipient you must not copy, distribute or use this email or the information contained in it for any purpose other than to notify us. If you have received this message in error, please notify Charles River immediately, and delete it from your system. From LSebree <@t> uwhealth.org Tue Jul 29 15:14:45 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Tue Jul 29 15:14:49 2008 Subject: [Histonet] cut control slides References: <03C921A1EAF7F541B16543F6EC6A4B3701D3A93D@giamail2.Gia.com> Message-ID: It depends entirely on the antibody they are to be used for. I'm sure if you look into archives you'll be able to pull up a number of e-mails regarding different antigens and the lability of their epitopes. That aside, its entirely possible to maintain an inventory of pre-cut control slides either at room temperature or frozen. Again, the archives should be able to give you an idea of which antigens present a problem if control slides are stored at RT. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Amber McKenzie Sent: Tue 7/29/2008 1:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cut control slides How long do you think cut control slides will last? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sheila_adey <@t> hotmail.com Tue Jul 29 15:27:36 2008 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Tue Jul 29 15:27:41 2008 Subject: [Histonet] Immuno Stainer In-Reply-To: <37DEF9AF72994947AF693956A59B9B660127FF4A@mailbe03.mc.vanderbilt.edu> References: <37DEF9AF72994947AF693956A59B9B660127FF4A@mailbe03.mc.vanderbilt.edu> Message-ID: we have the Biogenex i6000, it also has true random access, you can load up to 5 runs. However, if your staff does not have a good grasp of IHCs it may be difficult to operate. It is completely open. You can put any antibody or detection kit on it. Sheila Adey HT MLTPort Huron HospitalMichigan> Date: Tue, 29 Jul 2008 12:30:49 -0500> From: kenneth.a.troutman@Vanderbilt.Edu> To: Histonet@lists.utsouthwestern.edu> Subject: [Histonet] Immuno Stainer> CC: > > Hi Sandy,> > We use the Leica BondMax. It is the only stainer that I know of that will allow you to start a run and then load more slides while the others are running. (Twice, actually.) As for the multiple detection kits, I believe with the model we have, you have to use at least one of their reagents on their detection kit to run the protocols. (You can just use the hematoxylin if you don't want to use anything else.) There is also, I believe a research version of this instrument that may be more flexible. > > Hope this helps.> > Ashley Troutman BS, HT(ASCP)QIHC> Histopathology Laboratory> Department of Pathology> Vanderbilt University Medical Center> Nashville, TN> > Message: 1> Date: Mon, 28 Jul 2008 12:07:19 -0500> From: "Sandra Cheasty" > Subject: [Histonet] Immuno Stainer> To: "histonet@lists.utsouthwestern.edu"> > Message-ID:> b9g15eVF2DwEAAAAA@svm.vetmed.wisc.edu>> Content-Type: text/plain; charset="us-ascii"> > Any suggestions re: an "open" Immunostainer that allows multiple detection kits, and also allows starting a new run before the previous run has completed? I work with veterinary diagnostic and research tissues.> > > > Thank you,> > Sandy> > UW-Madison > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ From ht.ascp <@t> yahoo.com Tue Jul 29 15:28:52 2008 From: ht.ascp <@t> yahoo.com (Jon Google) Date: Tue Jul 29 15:28:55 2008 Subject: [Histonet] Dictation systems In-Reply-To: Message-ID: <366247.75524.qm@web59902.mail.ac4.yahoo.com> We are in the process of upgrading our LIS. We are looking at moving our dictation system from our current Dictaphone analog system. We would like to use a digital system that can support VOIP.? The problem we are running into is trying to find one that supports a hands free control that our grossing pathologists need. ? Does anyone have recommendations on digital systems that support foot controls? ? Thanks Jon From Ronald.Houston <@t> nationwidechildrens.org Tue Jul 29 15:29:19 2008 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Tue Jul 29 15:29:43 2008 Subject: [Histonet] Histology Specialist Vacancy Message-ID: <979FF5962E234F45B06CF0DB7C1AABB20FEC223A@chi2k3ms01.columbuschildrens.net> Histology Specialist II - Anatomic Pathology Nationwide Children's Hospital- Columbus, OH Nationwide Children's Hospital, a 353-bed facility is among the most comprehensive free-standing, pediatric academic medical centers in the nation. We host the nation's largest neonatal program with 101 licensed on-site neonate beds, and an additional 66 beds under management with local Level III and II nurseries. US News & World Report also named us one of America's Best Children's Hospitals in all 7 ranked specialties. Nationwide Children's seeks a full-time, day shift Anatomic Pathology (AP) Specialist to be responsible for maintaining the daily technical and procedural operations of the department, particularly instrument function; researching, developing and implementing new or improved procedures and instrumentation and performing quality control, quality improvement and regulatory compliance tasks. Minimum Qualifications: * Bachelor's in a related science * Minimum of 5 years bench work experience in a similar laboratory * Certification of ASCP or equivalent * Effective interpersonal skills Relocation assistance is available. Salary is commensurate with experience. For more information & to apply please visit us on-line at: http://www.NationwideChildrens.org Nationwide Children's Hospital is an equal opportunity employer that values diversity. Candidates of diverse backgrounds are encouraged to apply. Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5450 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From HornHV <@t> archildrens.org Tue Jul 29 15:31:33 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Jul 29 15:31:31 2008 Subject: [Histonet] Dictation systems In-Reply-To: <366247.75524.qm@web59902.mail.ac4.yahoo.com> References: <366247.75524.qm@web59902.mail.ac4.yahoo.com> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82D53@EMAIL.archildrens.org> I would be interested in this information as well Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jon Google Sent: Tuesday, July 29, 2008 3:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dictation systems We are in the process of upgrading our LIS. We are looking at moving our dictation system from our current Dictaphone analog system. We would like to use a digital system that can support VOIP.? The problem we are running into is trying to find one that supports a hands free control that our grossing pathologists need. ? Does anyone have recommendations on digital systems that support foot controls? ? Thanks Jon _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From christina.thurby <@t> bms.com Tue Jul 29 15:53:23 2008 From: christina.thurby <@t> bms.com (Christina Thurby) Date: Tue Jul 29 15:52:14 2008 Subject: [Histonet] Pharmaceutical companies that perform IHC and GLP regulations??? In-Reply-To: <200807291705.m6TH5p5k024476@meusplapp01.net.bms.com> References: <200807291705.m6TH5p5k024476@meusplapp01.net.bms.com> Message-ID: <488F8343.1060608@bms.com> Hello, Can any pharmaceutical people comment on how you are handleing SOP's that are GLP compliant (21CFR Part 58)? We currently are considering whether we need to write an individual SOP for each antibody that we perform by IHC. We have general IHC SOP's in place now and have an Antibody Specific Protol for each antibody in a Word document. Any comments are welcome. Thanks, Christina Thurby 812-429-8097 From liz <@t> premierlab.com Tue Jul 29 16:30:21 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Jul 29 16:30:26 2008 Subject: [Histonet] Pharmaceutical companies that perform IHC and GLPregulations??? In-Reply-To: <488F8343.1060608@bms.com> References: <200807291705.m6TH5p5k024476@meusplapp01.net.bms.com> <488F8343.1060608@bms.com> Message-ID: We have a bunch of general SOP's then we do have a separate SOP for each antibody for all of our GLP IHC. We also had to write a detailed SOP on the dako autostainer and we then we performed a standard IOQ on the instrument to validate it. It's a lot of work. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Christina Thurby Sent: Tuesday, July 29, 2008 2:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pharmaceutical companies that perform IHC and GLPregulations??? Hello, Can any pharmaceutical people comment on how you are handleing SOP's that are GLP compliant (21CFR Part 58)? We currently are considering whether we need to write an individual SOP for each antibody that we perform by IHC. We have general IHC SOP's in place now and have an Antibody Specific Protol for each antibody in a Word document. Any comments are welcome. Thanks, Christina Thurby 812-429-8097 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Tue Jul 29 16:35:00 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Jul 29 16:35:09 2008 Subject: [Histonet] Dictation systems In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82D53@EMAIL.archildrens.org> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A38B1@IS-E2K3.grhs.net> As would I. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Tuesday, July 29, 2008 3:32 PM To: ht.ascp@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dictation systems I would be interested in this information as well Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jon Google Sent: Tuesday, July 29, 2008 3:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dictation systems We are in the process of upgrading our LIS. We are looking at moving our dictation system from our current Dictaphone analog system. We would like to use a digital system that can support VOIP.? The problem we are running into is trying to find one that supports a hands free control that our grossing pathologists need. ? Does anyone have recommendations on digital systems that support foot controls? ? Thanks Jon _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Herrick.James <@t> mayo.edu Tue Jul 29 17:47:22 2008 From: Herrick.James <@t> mayo.edu (Herrick, James L.) Date: Tue Jul 29 17:47:44 2008 Subject: [Histonet] Question on Aluminum Staining Message-ID: Hi everyone, We are having trouble running ASA (acid solochrome azurine) stains on human iliac crest bone biopsies (embedded in plastic resin--GMA) for the detection of aluminum deposits. Our negatives are showing a lot of blue around the outer edges of the specimen. Although we cannot see staining around the trabecular bone, we should also not see it around the periphery of the bone specimen. The positive, on the other hand, looks very good. Has anyone had experience with this type of stain, and if so, would you happen to know what we may be doing wrong? The recipe we use for our stain is .2 gms. of Chromeazural B in 20mL of dH2O with a pH @ 5.0 (We incubate at room temp for 18hrs.), destain with 95% methanol, rinse in dH2O, dehydrate, clear and coverslip. Also, another problem we have encountered in the past, is a gold discoloring of the specimen. Have you seen this problem before, and if so, do you know how to correct it? Any help we can get with this dilemma is very much appreciated. Thanks again, Jim From bturdi <@t> gmail.com Tue Jul 29 22:31:27 2008 From: bturdi <@t> gmail.com (subat turdi) Date: Tue Jul 29 22:31:32 2008 Subject: [Histonet] Processing of mouse hearts Message-ID: <4e61b8250807292031y53350bf5p97bb65b40e2bfe2b@mail.gmail.com> Dear all, Does anyone have a detailed protocol for processing mouse hearts for paraffin slides? I am new to this technique and having various troubles in getting good histology. We don't have a automatic processer and all is manual. Thanks in advance. Subat Turdi School of Pharmacy University of Wyoming Laramie, Wyoming From sprice2003 <@t> gmail.com Wed Jul 30 01:27:47 2008 From: sprice2003 <@t> gmail.com (Sally Price) Date: Wed Jul 30 01:27:55 2008 Subject: [Histonet] Immuno Stainer Message-ID: Sandra: As far as I know, there is only one stainer that can do continuous-access and allows you to mix-and-match detection systems from different sources. I read about it in a paper that you can download at - - http://www.mlo-online.com/articles/0108/0108product_focus.pdf Cheers, Sally ---------------------------------------------------------------------- Message: 1 Date: Mon, 28 Jul 2008 12:07:19 -0500 From: "Sandra Cheasty" Subject: [Histonet] Immuno Stainer To: "histonet@lists.utsouthwestern.edu" Any suggestions re: an "open" Immunostainer that allows multiple detection kits, and also allows starting a new run before the previous run has completed? I work with veterinary diagnostic and research tissues. Thank you, Sandy UW-Madison From tifei <@t> foxmail.com Wed Jul 30 02:44:07 2008 From: tifei <@t> foxmail.com (tf) Date: Wed Jul 30 02:44:32 2008 Subject: [Histonet] Storage of WGA-HRP for work solution (axon tracing) References: <4e61b8250807292031y53350bf5p97bb65b40e2bfe2b@mail.gmail.com> Message-ID: <200807301544022278699@foxmail.com> Hi All: Consistent with my previous discussion on WGA-HRP use for anterograde tracing, I have purchased 1 mg L7017 from Sigma. Given the tiny amount within the tube, do anyone have the experience in choosing a proper dilution factor for storage? I think the best way is to dilute WGA-HRP into work solution, for example, 1% for axon tracing, and stored in separate tube (5 ul per tube)? Or there are any other tricks? They do not provide this on information sheet. http://www.sigmaaldrich.com/sigma/product%20information%20sheet/l7017pis.pdf Thanks very much. 2008-07-30 tf From masaakikitada2007 <@t> gmail.com Wed Jul 30 03:25:57 2008 From: masaakikitada2007 <@t> gmail.com (Masaaki Kitada) Date: Wed Jul 30 03:25:19 2008 Subject: [Histonet] phorphprylated-STAT3 antibody Message-ID: Dear all, I've tried to use anti-phosphorylated-STAT3 antibodies in 10um thick mouse brain cryosections for immunofluorescence with antigen retrieval but found dirty result or nothing. Does anyone know good antibodies against pSTAT3? Or, does anyone let me know the appropriate protocol for immunofluorescence using following antibodies? Antibodies Cell signaling: tyr705 9145S some nuclei (expected) overall cellular processes (unexpected) tyr705 9131S nothing detected BD Biosciences: Ser727 612542 nothing detected Sample: Mouse brain section fix with 4% paraformaldehyde in 0.1M PB and post-fix with the same fixatives for 1~2 ON 10-um-thick cryosection Antigen retrieval condition: prewarm the solution incubate for 20 min stay for 10 min cool down with running water for 10 min antigen retrieval solution: 5mM Tris-HCl / 2mM EDTA, pH 8.0 Many thanks, Masaaki Kitada Tohoku University Graduate School of Medicine From cmiller <@t> physlab.com Wed Jul 30 08:01:12 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Wed Jul 30 08:02:21 2008 Subject: [Histonet] CAP Inspection last Friday. Message-ID: <001201c8f244$582bc2e0$3d02a8c0@plab.local> I survived it! LOL My inspector was a very charming Dr. originally from Chile. I forget her name already! I was her first inspection and she went thru each question not skipping one. She had me pull records to show that I was following each of my procedures. And one year was not enough "08" I had to pull' 07" records and show her I was compliant since my last inspection in "06'. She left no rock unturned! I was exhausted after more than 6.5 hours in my office just her, myself and my books. She also went into my lab and spoke with my staff, asked questions about procedures and wouldn't let me answer, she wanted to know what they knew and their processes. Making sure that policy was in fact routine practice. She was very sharp, very pleasant and very thorough. I am so happy it's over. I received 2 phase II. She found an improperly labeled reagent (Alcian blue had no fill date on the coplin jar) and another one for inadequate work space (our lab is in dire need of remodeling) we are on top of each other. Good luck to those of you still waiting for your inspectors to arrive. Cheri Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From Lynne.Bell <@t> hitchcock.org Wed Jul 30 09:18:23 2008 From: Lynne.Bell <@t> hitchcock.org (Bell, Lynne) Date: Wed Jul 30 09:18:31 2008 Subject: [Histonet] CAP Inspection last Friday. In-Reply-To: <001201c8f244$582bc2e0$3d02a8c0@plab.local> Message-ID: Congratulations on surviving your CAP inspection. We are anxiously awaiting ours. Our three month window ends September 30. As each day goes past 9:00 and inspectors are not banging on the door, we all take a deep breath and then do it all over again the next day!! Lynne Bell, HT (ASCP) Lead Histologist Central Vermont Hospital P. O. Box 547 Barre, VT 05641 802-371-4923 From gayle.callis <@t> bresnan.net Wed Jul 30 10:12:32 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Wed Jul 30 10:12:36 2008 Subject: [Histonet] Storage of WGA-HRP for work solution (axon tracing) References: <4e61b8250807292031y53350bf5p97bb65b40e2bfe2b@mail.gmail.com> <200807301544022278699@foxmail.com> Message-ID: <001801c8f256$b0b48930$6401a8c0@Sunney> Call Sigma. However we do NOT predilute and store it, and it will be extremely difficult to store 5 ul in a tube, let alone even see that amount after you pipette it. We do the dilution at the time of staining. If reconstituting a dry WGA - I assume this is what you need, I would make it 1 mg/ml for ease of use. However, Sigma should have given you instructions on what to use for reconstitution in that data sheet. One needs to be careful with lectins i.e. the buffer used. Gayle M. Callis HTL/HT/MT(ASCP) ----- Original Message ----- From: "tf" To: "histonet@lists.utsouthwestern.ed" Sent: Wednesday, July 30, 2008 1:44 AM Subject: [Histonet] Storage of WGA-HRP for work solution (axon tracing) > Hi All: > > Consistent with my previous discussion on WGA-HRP use for anterograde > tracing, I have purchased 1 mg L7017 from Sigma. > Given the tiny amount within the tube, do anyone have the experience in > choosing a proper dilution factor for storage? > I think the best way is to dilute WGA-HRP into work solution, for example, > 1% for axon tracing, and stored in separate tube (5 ul per tube)? > Or there are any other tricks? > > They do not provide this on information sheet. > http://www.sigmaaldrich.com/sigma/product%20information%20sheet/l7017pis.pdf > > Thanks very much. > > > 2008-07-30 > > > > tf > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bturdi <@t> gmail.com Wed Jul 30 10:49:19 2008 From: bturdi <@t> gmail.com (subat turdi) Date: Wed Jul 30 10:49:24 2008 Subject: Fwd: [Histonet] Processing of mouse hearts In-Reply-To: <4e61b8250807300832o64c19bdbn95c2ab2e15850a@mail.gmail.com> References: <4e61b8250807292031y53350bf5p97bb65b40e2bfe2b@mail.gmail.com> <001001c8f255$6d8761b0$6401a8c0@Sunney> <4e61b8250807300832o64c19bdbn95c2ab2e15850a@mail.gmail.com> Message-ID: <4e61b8250807300849i1800e36qfa73be6841d2d818@mail.gmail.com> Folks, I appreciate your responses very much. This is the protocol I use. Please give me some advice what has gone wrong. Animals weighed and anesthetized w/ ketamine-xylazine combo.Chest cavity opened quickly and the heart was exposed. IVC was found and KCl was injected iv to arrest the heart was in diastole.The heart was taken out fast and placed in a cold PBS to wash out the excess blood. (Perfusion w/ PFA through aorta is optional) .The heart was cut into several ~2 mm thick doughnuts with special interest on the ventriculum then put into 4% PFA solution for 24h at 4 degrees.Then the tissue was rinsed with running water for 30min.Then subjected to graded alcohols. 70% 30min 95% 20min X2 100% 20minX2 Xylene 20min Xylene 20 min Infiltrate in paraplast: Station 1: 45min Station 2 : 45min Embedding with paraplast My major problem is that a lot of times I get dry blocks. The ribbons i get often comes without the tissue and breaks into shards. Subat On 7/30/08, Gayle Callis wrote: > > If you tell us how YOU do it, then we can help modify your protocol. > ----- Original Message ----- From: "subat turdi" > To: > Sent: Tuesday, July 29, 2008 9:31 PM > Subject: [Histonet] Processing of mouse hearts > > > Dear all, >> >> Does anyone have a detailed protocol for processing mouse hearts for >> paraffin slides? I am new to this technique and having various troubles in >> getting good histology. We don't have a automatic processer and all is >> manual. Thanks in advance. >> >> Subat Turdi >> >> School of Pharmacy >> University of Wyoming >> Laramie, Wyoming >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > From nasonkini <@t> mail.nih.gov Wed Jul 30 11:03:52 2008 From: nasonkini <@t> mail.nih.gov (Igor Nasonkin) Date: Wed Jul 30 11:04:03 2008 Subject: [Histonet] Unsubscribe /excellent group, too many emals though, clutter my emal Message-ID: Please unsubscribe for now, can't handle too many emails Can you make it more selective? Thank you Dr. Igor O. Nasonkin Research Fellow National Institutes of Health/NEI 9000 Rockville Pike, MSC 1864 Bldg 10, Room 10B11 Bethesda, MD 20892 Tel: 301-443-7398 ?work 617-388-4104 ?cell Fax 301-480-1769 email: nasonkini@mail.nih.gov http://www.nei.nih.gov/intramural/nnrl.asp From rosenfeldtek <@t> hotmail.com Wed Jul 30 11:07:42 2008 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Wed Jul 30 11:07:46 2008 Subject: [Histonet] Processing of mouse hearts In-Reply-To: <4e61b8250807300849i1800e36qfa73be6841d2d818@mail.gmail.com> References: <4e61b8250807292031y53350bf5p97bb65b40e2bfe2b@mail.gmail.com> <001001c8f255$6d8761b0$6401a8c0@Sunney> <4e61b8250807300832o64c19bdbn95c2ab2e15850a@mail.gmail.com> <4e61b8250807300849i1800e36qfa73be6841d2d818@mail.gmail.com> Message-ID: It sounds like over processing to me. Mouse tissue is easy to over process. First check the TEMPERATURE of your paraffin pots--they may be running too hot. Cutting your heart into thin 2mm doughnuts will make the tissue process faster. I just cut the heart into ventricular half and atrial half--then process the whole thing. Works great. Jerry Ricks Research Scientist University of Washington Department of Pathology > Date: Wed, 30 Jul 2008 09:49:19 -0600 > From: bturdi@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: Fwd: [Histonet] Processing of mouse hearts > > Folks, I appreciate your responses very much. > This is the protocol I use. Please give me some advice what has gone wrong. > Animals weighed and anesthetized w/ ketamine-xylazine combo.Chest cavity > opened quickly and the heart was exposed. IVC was found and KCl was injected > iv to arrest the heart was in diastole.The heart was taken out fast and > placed in a cold PBS to wash out the excess blood. (Perfusion w/ PFA through > aorta is optional) .The heart was cut into several ~2 mm thick doughnuts > with special interest on the ventriculum then put into 4% PFA solution for > 24h at 4 degrees.Then the tissue was rinsed with running water for > 30min.Then subjected to graded alcohols. > > 70% 30min > > 95% 20min X2 > > 100% 20minX2 > > Xylene 20min > > Xylene 20 min > Infiltrate in paraplast: > Station 1: 45min > Station 2 : 45min > Embedding with paraplast > > My major problem is that a lot of times I get dry blocks. The ribbons i get > often comes without the tissue and breaks into shards. > > Subat > > > On 7/30/08, Gayle Callis wrote: > > > > If you tell us how YOU do it, then we can help modify your protocol. > > ----- Original Message ----- From: "subat turdi" > > To: > > Sent: Tuesday, July 29, 2008 9:31 PM > > Subject: [Histonet] Processing of mouse hearts > > > > > > Dear all, > >> > >> Does anyone have a detailed protocol for processing mouse hearts for > >> paraffin slides? I am new to this technique and having various troubles in > >> getting good histology. We don't have a automatic processer and all is > >> manual. Thanks in advance. > >> > >> Subat Turdi > >> > >> School of Pharmacy > >> University of Wyoming > >> Laramie, Wyoming > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Keep your kids safer online with Windows Live Family Safety. http://www.windowslive.com/family_safety/overview.html?ocid=TXT_TAGLM_WL_family_safety_072008 From khbarr <@t> mdanderson.org Wed Jul 30 11:40:06 2008 From: khbarr <@t> mdanderson.org (khbarr@mdanderson.org) Date: Wed Jul 30 11:40:30 2008 Subject: [Histonet] Vacant Positions Message-ID: The University of Texas M. D. Anderson Cancer Center in Houston, Texas has three histology technician vacancies. Interested candidates can call Dianna Menard @ 713-745-6184 or apply online at www.mdanderson.org. Kaye Barr, HT (ASCP) Laboratory Manager - Histology Department Division of Pathology & Laboratory Medicine 713-792-5366 khbarr@mdanderson.org From derek.papalegis <@t> tufts.edu Wed Jul 30 11:52:40 2008 From: derek.papalegis <@t> tufts.edu (Derek Papalegis) Date: Wed Jul 30 11:52:43 2008 Subject: Fwd: [Histonet] Processing of mouse hearts In-Reply-To: <4e61b8250807300849i1800e36qfa73be6841d2d818@mail.gmail.com> References: <4e61b8250807292031y53350bf5p97bb65b40e2bfe2b@mail.gmail.com> <001001c8f255$6d8761b0$6401a8c0@Sunney> <4e61b8250807300832o64c19bdbn95c2ab2e15850a@mail.gmail.com> <4e61b8250807300849i1800e36qfa73be6841d2d818@mail.gmail.com> Message-ID: <48909C58.4030308@tufts.edu> This is a common problem with mouse tissue. The tissue is being over processed. There are a few things that you can do to remedy this. One is to have a dedicated processing schedule for each different tissue that you process. Although this is ideal, it is often times impossible to realistically do this. If your lab is processing a variety of different mouse tissues at the same time, you will frequently encounter over processed tissue. Although not ideal, a way to get good sections is to rough into the blocks and before you put them on your cold tray, place them in your heated water bath for a few seconds. This will rehydrate the tissue enough so you can get good sections. If you do this often enough, you will get a "feel" for how long you need to leave them in your water bath. It seems like you have a few unnecessary steps in how you prepare these hearts. Is there a specific reason why you use cold PBS to rinse the blood out of the heart? Wouldn't formalin rinse the blood off just as effectively as PBS while also starting the fixation process more quickly? What is your reasoning for fixing at 4 degrees instead of at room temp? Lowering the temp slows down the fixation process. The 30 minute running water rinse after fixation is unnecessary as well. Feel free to contact me if you have additional questions. Derek Derek Papalegis HT (ASCP) Histotechnician Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 subat turdi wrote: > Folks, I appreciate your responses very much. > This is the protocol I use. Please give me some advice what has gone wrong. > Animals weighed and anesthetized w/ ketamine-xylazine combo.Chest cavity > opened quickly and the heart was exposed. IVC was found and KCl was injected > iv to arrest the heart was in diastole.The heart was taken out fast and > placed in a cold PBS to wash out the excess blood. (Perfusion w/ PFA through > aorta is optional) .The heart was cut into several ~2 mm thick doughnuts > with special interest on the ventriculum then put into 4% PFA solution for > 24h at 4 degrees.Then the tissue was rinsed with running water for > 30min.Then subjected to graded alcohols. > > 70% 30min > > 95% 20min X2 > > 100% 20minX2 > > Xylene 20min > > Xylene 20 min > Infiltrate in paraplast: > Station 1: 45min > Station 2 : 45min > Embedding with paraplast > > My major problem is that a lot of times I get dry blocks. The ribbons i get > often comes without the tissue and breaks into shards. > > Subat > > > On 7/30/08, Gayle Callis wrote: > >> If you tell us how YOU do it, then we can help modify your protocol. >> ----- Original Message ----- From: "subat turdi" >> To: >> Sent: Tuesday, July 29, 2008 9:31 PM >> Subject: [Histonet] Processing of mouse hearts >> >> >> Dear all, >> >>> Does anyone have a detailed protocol for processing mouse hearts for >>> paraffin slides? I am new to this technique and having various troubles in >>> getting good histology. We don't have a automatic processer and all is >>> manual. Thanks in advance. >>> >>> Subat Turdi >>> >>> School of Pharmacy >>> University of Wyoming >>> Laramie, Wyoming >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From luke.perkocha <@t> ucsf.edu Wed Jul 30 12:03:50 2008 From: luke.perkocha <@t> ucsf.edu (Luke A Perkocha) Date: Wed Jul 30 12:04:50 2008 Subject: [Histonet] Grossing of complex specimens by PAs and/or residents in pathology Message-ID: <6.2.3.4.2.20080730092835.0204d458@exchange.ucsf.edu> Greetings, The use of Pathology Assistants to do virtually all of the grossing of even complex pathology specimens, under the supervision of a pathologist is becoming commonplace in larger practices, and many are convinced it provides a more consistent, higher quality gross dissection and sampling, as long as the PAs are properly qualified, trained and supervised and have pathologist back-up. Conversely, in most pathology training programs, the residents do most or all of the grossing as part of their training (sometimes with the exception of small biopsies). However, this is counter-intuitive from a patient safety standpoint: i.e. your least experienced people, who are interrupted by other concurrent responsibilities and rotate in and out on a scheduled bases are doing the part of pathology which is most critical to patient safety and can't be replicated if done wrong. We are considering tweaking our program in some ways to improve patient safety in the gross room and reduce errors and would like to see what others do and think. The concept being tested is that more of the grossing of complex specimens should be done by PAs and experienced residents (not fresh trainees who are trying to learn all aspects of pathology and lab at the same time and are prone to errors) and the training of residents should also include aspects of directing a gross pathology lab (which requires knowing how to gross, but also other things), rather than just grossing alone. Could you help with the answers to these questions if you have a residency training program in pathology: * Do PAs gross complex specimens? * Are PAs involved in training residents or vice-versa? * What is the salary for PAs who gross complex specimens? * Do you have any other innovative ways to reduce gross room errors by residents in training or to teach gross pathology to same? Luke A. Perkocha, MD, MBA Associate Professor of Pathology and Dermatology Associate Director of Surgical Pathology UC San Francisco Helen Diller Family Comprehensive Cancer Center 1600 Divisadero Avenue, Box 1785 San Francisco, CA 94143-1785 office: 415 885-7254 cell: 415 509-6442 From tifei <@t> foxmail.com Wed Jul 30 12:11:21 2008 From: tifei <@t> foxmail.com (tf) Date: Wed Jul 30 12:11:38 2008 Subject: [Histonet] PSA_NCAM immunohistochemistry Message-ID: <200807310111158852149@foxmail.com> Dear All: Just wonder anyone have worked on PSA_NCAM IHC of frozen sections? How about 1:1000 mouse anti-PSA NCAM from millipore to stain 30 um brain sections? And, do you need to add triton into the PBS, to "pore" the membrane? I think PSA-NCAM is distributed on the cell membrane, thus PBS may work? Protocol: 1. Rehydration. PBS 2. Mouse-anti PSA-NCAM 1:1000 overnight + Blocking simultaneously. 3. 3* PBS 4. Goat-anti mouse conjugated with 488 fluroscein 5. 3 * PBS 6. DAPI 7. PBS wash 8. mount Any other comments on this? especially the time & concentration for first antibody? thx, 2008-07-31 tf From zodiac29 <@t> comcast.net Wed Jul 30 12:16:10 2008 From: zodiac29 <@t> comcast.net (zodiac29@comcast.net) Date: Wed Jul 30 12:16:15 2008 Subject: [Histonet] Alcian Yellow Message-ID: <073020081716.1090.4890A1DA000B321A000004422215575474C7CD0C0E070B0196@comcast.net> Hello all, I wanted to get some feed back from anyone using Alcian yellow as opposed to Geimsa for staining H.Pylori. Right now we are using Gemisa for detection of H.Pylori, and are considering changing to Alcian Yellow. Just wanted your opinion of what is most preferred by the pathologists. Thanks in Advance Jenny From Erin.Martin <@t> ucsf.edu Wed Jul 30 12:31:51 2008 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Wed Jul 30 12:36:43 2008 Subject: [Histonet] Alcohol fixation and immuno Message-ID: Hi everyone, We occassionally get blocks from outside labs that use alcohol as the fixative. Our pathologist told me that our immunos look pretty bad when we handle these cases in the same manner as our routine FFPE cases. He is most concerned with Ki67 and K/L. I tried them with no antigen retrieval but got no staining at all. Any thoughts? We only do derm. Thanks, Erin From Maxim_71 <@t> mail.ru Wed Jul 30 13:17:18 2008 From: Maxim_71 <@t> mail.ru (Maxim_71@mail.ru) Date: Wed Jul 30 13:19:28 2008 Subject: [Histonet] Processing of mouse hearts Message-ID: <1253698354.20080730221718@mail.ru> Subat: Here is my manual protocol for mouses tissues, that works very well for all mouse tissues (thickness no more than 3 mm): 1. Isopropanol 70% 30 min 2. Isopropanol 80% 30 min 3. Isopropanol 95% 30 min 4. Isopropanol 99% 30 min 5. Isopropanol:Mineral oil 5:1 50oC 60 min 6. Isopropanol:Mineral oil 2:1 50oC 60 min 7. Mineral oil 1.5 h (or overnight) 8. Paraffin 60oC 30 min 9. Paraffin 60oC 30 min 10. Paraffin 60oC 20 min 11. Paraffin 60oC 20 min. Advantage of isopropanol consists in that ethanol have "tide" effect and do tissues hard, but isopropanol (IPA) not. Mineral oil = paraffin with low molecular weight and the infiltration is better and gentle. Mineral oil is absolutely not toxic for personnel. Transitions between IPA, MO and paraffin are very gentle. Please let me know about results. If you need any more details please contact to me. Best wishes, Maxim Peshkov Russia, Taganrog. mailto:Maxim_71@mail.ru From jflinn <@t> gmu.edu Wed Jul 30 13:23:40 2008 From: jflinn <@t> gmu.edu (Jane M Flinn) Date: Wed Jul 30 13:23:54 2008 Subject: [Histonet] Re: -86 freezers In-Reply-To: References: Message-ID: WE are interested in buying a 30 cu ft -86 upright freezer. Recommnendations appreciated. Jane "Life is short - make haste to be kind" Dr. Jane Flinn Director, Biopsychology Program George Mason University, 3F5 4400 University Dr. Fairfax, VA 22030 Phone: 703-993-4107 Fax: 703-993-1359 ----- Original Message ----- From: "Martin, Erin" Date: Wednesday, July 30, 2008 1:31 pm Subject: [Histonet] Alcohol fixation and immuno > Hi everyone, > > We occassionally get blocks from outside labs that use alcohol as > the fixative. Our pathologist told me that our immunos look > pretty bad when we handle these cases in the same manner as our > routine FFPE cases. He is most concerned with Ki67 and K/L. I > tried them with no antigen retrieval but got no staining at all. > Any thoughts? We only do derm. > > Thanks, > Erin > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From zodiac29 <@t> comcast.net Wed Jul 30 13:33:44 2008 From: zodiac29 <@t> comcast.net (zodiac29@comcast.net) Date: Wed Jul 30 13:33:54 2008 Subject: [Histonet] Alcian Yelllow for H.pylori Message-ID: <073020081833.14431.4890B40800030A6B0000385F2215578674C7CD0C0E070B0196@comcast.net> Rosa and Ellen, Thank you for your quick responses : ) Does anyone happen to have the procedure for Alcian yellow for H.Pylori? Thanks again Jenny From ploykasek <@t> phenopath.com Wed Jul 30 13:38:14 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Wed Jul 30 13:40:27 2008 Subject: [Histonet] Alcohol fixation and immuno In-Reply-To: Message-ID: HI Erin. The best situation would be you could speak with this client, and persuade them to use formalin, too. Let them know your IHC protocols are optimized for FFPE (probably H&E's would look better, too). If this doesn't work, obtain some alcohol fixed control material and optimize your IHC for this material, too. You will need to try a variety of pretreatments & titers. In essence you will have 2 protocols for your IHC - 1 for FFPE & 1 for alcohol fixed. What fun! Good luck with working all this out. Patti Loykasek BS, HTL, QIHC Clinical IHC Supervisor PhenoPath Laboratories Seattle, WA > Hi everyone, > > We occassionally get blocks from outside labs that use alcohol as the > fixative. Our pathologist told me that our immunos look pretty bad when we > handle these cases in the same manner as our routine FFPE cases. He is most > concerned with Ki67 and K/L. I tried them with no antigen retrieval but got > no staining at all. Any thoughts? We only do derm. > > Thanks, > Erin > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From SHargrove <@t> urhcs.org Wed Jul 30 13:43:22 2008 From: SHargrove <@t> urhcs.org (SHargrove@urhcs.org) Date: Wed Jul 30 13:43:41 2008 Subject: [Histonet] Susie Hargrove is out of the office. Message-ID: I will be out of the office starting 07/30/2008 and will not return until 08/04/2008. I will respond to your message when I return. If immediate assistance is needed please call 3198. From PMonfils <@t> Lifespan.org Wed Jul 30 14:13:20 2008 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Jul 30 14:13:26 2008 Subject: Fwd: [Histonet] Processing of mouse hearts In-Reply-To: <48909C58.4030308@tufts.edu> Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835C43@LSRIEXCH1.lsmaster.lifespan.org> > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Derek Papalegis > Sent: Wednesday, July 30, 2008 12:52 PM > To: subat turdi > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: Fwd: [Histonet] Processing of mouse hearts > > This is a common problem with mouse tissue. The tissue is being over > processed. There are a few things that you can do to remedy this. One is > to have a dedicated processing schedule for each different tissue that > you process. Although this is ideal, it is often times impossible to > realistically do this. If your lab is processing a variety of different > mouse tissues at the same time, you will frequently encounter over > processed tissue. Although not ideal, a way to get good sections is to > rough into the blocks and before you put them on your cold tray, place > them in your heated water bath for a few seconds. This will rehydrate > the tissue enough so you can get good sections. If you do this often > enough, you will get a "feel" for how long you need to leave them in > your water bath. > > It seems like you have a few unnecessary steps in how you prepare these > hearts. Is there a specific reason why you use cold PBS to rinse the > blood out of the heart? Wouldn't formalin rinse the blood off just as > effectively as PBS while also starting the fixation process more > quickly? What is your reasoning for fixing at 4 degrees instead of at > room temp? Lowering the temp slows down the fixation process. The 30 > minute running water rinse after fixation is unnecessary as well. > > Feel free to contact me if you have additional questions. > Derek > > Derek Papalegis HT (ASCP) > Histotechnician > Division of Laboratory Animal Medicine > Tufts University > 136 Harrison Avenue > Boston, MA 02111 > phone: 617 636-2971 > fax: 617 636-8354 > > > > subat turdi wrote: > > Folks, I appreciate your responses very much. > > This is the protocol I use. Please give me some advice what has gone wrong. > > Animals weighed and anesthetized w/ ketamine-xylazine combo.Chest cavity > > opened quickly and the heart was exposed. IVC was found and KCl was injected > > iv to arrest the heart was in diastole.The heart was taken out fast and > > placed in a cold PBS to wash out the excess blood. (Perfusion w/ PFA through > > aorta is optional) .The heart was cut into several ~2 mm thick doughnuts > > with special interest on the ventriculum then put into 4% PFA solution for > > 24h at 4 degrees.Then the tissue was rinsed with running water for > > 30min.Then subjected to graded alcohols. > > > > 70% 30min > > > > 95% 20min X2 > > > > 100% 20minX2 > > > > Xylene 20min > > > > Xylene 20 min > > Infiltrate in paraplast: > > Station 1: 45min > > Station 2 : 45min > > Embedding with paraplast > > > > My major problem is that a lot of times I get dry blocks. The ribbons i get > > often comes without the tissue and breaks into shards. > > > > Subat > > > > > > On 7/30/08, Gayle Callis wrote: > > > >> If you tell us how YOU do it, then we can help modify your protocol. > >> ----- Original Message ----- From: "subat turdi" > >> To: > >> Sent: Tuesday, July 29, 2008 9:31 PM > >> Subject: [Histonet] Processing of mouse hearts > >> > >> > >> Dear all, > >> > >>> Does anyone have a detailed protocol for processing mouse hearts for > >>> paraffin slides? I am new to this technique and having various troubles in > >>> getting good histology. We don't have a automatic processer and all is > >>> manual. Thanks in advance. > >>> > >>> Subat Turdi > >>> > >>> School of Pharmacy > >>> University of Wyoming > >>> Laramie, Wyoming > >>> _______________________________________________ > >>> Histonet mailing list > >>> Histonet@lists.utsouthwestern.edu > >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >>> > >>> > > >> > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From nbhatia <@t> dermatology.wisc.edu Wed Jul 30 14:23:48 2008 From: nbhatia <@t> dermatology.wisc.edu (Neehar Bhatia) Date: Wed Jul 30 14:23:52 2008 Subject: [Histonet] Please Unsubscribe Message-ID: <20080730142348531.00000000760@derm-7W5SGF1> Please unsubscribe Thanks Neehar Bhatia Assistant Scientist Department of Dermatology 1300 University Avenue, Rm 445 Madison WI 53706 Ph. 608.263.7144 Fax. 608.263.5223 From jgutierrez <@t> precisionpath.us Wed Jul 30 14:36:42 2008 From: jgutierrez <@t> precisionpath.us (Juan Gutierrez) Date: Wed Jul 30 14:36:49 2008 Subject: [Histonet] Unsubscribe Message-ID: <46B933DABE5D477A9221C2DFDD634909@precisionpath.lcl> Please unsubscribe me from the list. Thank you, Juan C. Gutierrez, HT(ASCP) From carrolpb <@t> umdnj.edu Wed Jul 30 14:46:33 2008 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Wed Jul 30 14:47:12 2008 Subject: [Histonet] Unsubscribe In-Reply-To: <46B933DABE5D477A9221C2DFDD634909@precisionpath.lcl> References: <46B933DABE5D477A9221C2DFDD634909@precisionpath.lcl> Message-ID: <4890C519.6000307@umdnj.edu> Just for the record... do all of you "unsubscribers" know that you can click the link that's at the bottom of every email from this list and unsubscribe yourself? Just a tip! http://lists.utsouthwestern.edu/mailman/listinfo/histonet Juan Gutierrez wrote: > Please unsubscribe me from the list. > > > > Thank you, > > > > Juan C. Gutierrez, HT(ASCP) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From nealtaylor01 <@t> bellsouth.net Wed Jul 30 15:23:46 2008 From: nealtaylor01 <@t> bellsouth.net (nealtaylor01@bellsouth.net) Date: Wed Jul 30 15:23:52 2008 Subject: [Histonet] (no subject) Message-ID: <073020082023.22722.4890CDD20006E9C1000058C222216125569B0A02D2089B9A019C04040A0DBFCECF9D0104970E9B040E0A02@att.net> Please unsubscribe - thanks From zodiac29 <@t> comcast.net Wed Jul 30 15:46:14 2008 From: zodiac29 <@t> comcast.net (zodiac29@comcast.net) Date: Wed Jul 30 15:46:22 2008 Subject: [Histonet] Alcian Yellow Message-ID: <073020082046.2974.4890D3160001B76500000B9E2216551406C7CD0C0E070B0196@comcast.net> Thank you everyone for your swift replies concerning the Alcian Yellow stain, it is much appreciated : ) Jenny From gayle.callis <@t> bresnan.net Wed Jul 30 16:04:45 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Wed Jul 30 16:04:49 2008 Subject: [Histonet] Alcian Yelllow for H.pylori References: <073020081833.14431.4890B40800030A6B0000385F2215578674C7CD0C0E070B0196@comcast.net> Message-ID: <001c01c8f287$e4f41f90$6401a8c0@Sunney> You can buy the staining kits from Master Tech and probably Newcomer's or Poly Scientific certainly easier than making it up, plus kits come with directions. I belive staining protocols have been posted on Histonet several times - you can check Histonet Archives Gayle M. Callis HTL/HT/MT(ASCP) ----- Original Message ----- From: To: Sent: Wednesday, July 30, 2008 12:33 PM Subject: [Histonet] Alcian Yelllow for H.pylori > > Rosa and Ellen, Thank you for your quick responses : ) > Does anyone happen to have the procedure for Alcian yellow for H.Pylori? > > Thanks again > Jenny > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Wed Jul 30 18:13:46 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Jul 30 18:13:57 2008 Subject: [Histonet] Unsubscribe In-Reply-To: <4890C519.6000307@umdnj.edu> Message-ID: Yep Nice and easy BUT I WOULD NEVER CONSIDER IT Histonet is too good! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Peter Carroll Sent: Thursday, 31 July 2008 5:47 AM Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Unsubscribe Just for the record... do all of you "unsubscribers" know that you can click the link that's at the bottom of every email from this list and unsubscribe yourself? Just a tip! http://lists.utsouthwestern.edu/mailman/listinfo/histonet Juan Gutierrez wrote: > Please unsubscribe me from the list. > > > > Thank you, > > > > Juan C. Gutierrez, HT(ASCP) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Wed Jul 30 18:41:31 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Jul 30 18:41:36 2008 Subject: [Histonet] Alcian Yellow In-Reply-To: <073020082046.2974.4890D3160001B76500000B9E2216551406C7CD0C0E070B0196@comcast.net> Message-ID: I never saw any! Why? Please use "reply to all" so that everyone on the list can see your contributions. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of zodiac29@comcast.net Sent: Thursday, 31 July 2008 6:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Alcian Yellow Thank you everyone for your swift replies concerning the Alcian Yellow stain, it is much appreciated : ) Jenny _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From sjchtascp <@t> yahoo.com Wed Jul 30 20:38:51 2008 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Wed Jul 30 20:38:55 2008 Subject: [Histonet] Looking for a Position Message-ID: <62903.6139.qm@web38207.mail.mud.yahoo.com> I'm looking for a full time?HT position im the Madison, WI area.? Any information, especially research facilities that employ HT's would be appreciated. ? Thanks, ? ? From Rcartun <@t> harthosp.org Thu Jul 31 09:45:59 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Jul 31 09:46:13 2008 Subject: [Histonet] Alcohol fixation and immuno In-Reply-To: References: Message-ID: <489197E7020000770000484A@gwmail6.harthosp.org> Are they using pure alcohol for fixation? I do IHC for a dermpath lab that uses alcoholic formalin and their tissue stains very well. The only difference is we have had to cut-back on our antigen retrieval for certain proteins. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Martin, Erin" 07/30/08 1:31 PM >>> Hi everyone, We occassionally get blocks from outside labs that use alcohol as the fixative. Our pathologist told me that our immunos look pretty bad when we handle these cases in the same manner as our routine FFPE cases. He is most concerned with Ki67 and K/L. I tried them with no antigen retrieval but got no staining at all. Any thoughts? We only do derm. Thanks, Erin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Thu Jul 31 09:55:07 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Thu Jul 31 09:55:14 2008 Subject: [Histonet] PSLIM Slide Labeling Message-ID: <4891D24B.1060800@pathology.washington.edu> Does anyone have the Pslim slide labeler from AccuPlace. http://www.accuplace.com/PSLIM.asp It sounds really nice for our work environment, but I'd like some real world feedback. Victor -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From mcquilk <@t> tcd.ie Thu Jul 31 10:23:06 2008 From: mcquilk <@t> tcd.ie (Keith Mc Quillan) Date: Thu Jul 31 10:23:11 2008 Subject: [Histonet] Basic staining questions Message-ID: <94847270807310823p72b56af7vd1481f58a1641edb@mail.gmail.com> Hi, I am pretty new to staining (as in i have only ever tried it once) and would appreciate any help you can give me with regard to processing my tissue and staining. I will be processing mouse brains in the coming weeks with the intention of staining for amyloid, and want to check about the correct method for fixing this tissue. In our lab, we have always snap frozen tissue in OCT directly after taking it, no sucrose gradients or aldehydes etc.However, i have noticed from reading the literature that most labs seem to fix the tissue in 4% paraformaldehyde before fixing in paraffin. Does it make any difference as to which protocol is used? I am reluctant to change unless i have good reason to. I am planning on perfusing the animals with PBS before taking half the brain for immunohistochemistry (in OCT) and the other half for ELISA/RNA etc and so can't perfuse with PFA. My other question relates to the staining for amyloid beta itself. I have noticed that a number of protocols incorporate a step to treat the sections with 70% formic acid prior to staining. Is this neccessary for the staining of amyloid, and if so, what advantages does it offer. Any help anyone can provide me with would be greatly appreciated Thanks Keith Mc Quillan Trinity College Dublin From Kimberly.Marshall <@t> ahss.org Thu Jul 31 10:34:16 2008 From: Kimberly.Marshall <@t> ahss.org (Marshall, Kimberly) Date: Thu Jul 31 10:34:32 2008 Subject: [Histonet] Sakura V.I.P Message-ID: Hello all, I am wondering if anyone has had to change a Sakura Tissue-Tek Processor from counter top or side by side, to a up-right or stand alone. I have a very small lab and need to make room for a new processor and it will all fit if there is a way to change it. Any advise or info would be helpful. Kimberly Marshall H.T. (ASCP) Metroplex Hospital ============================================================================== The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ============================================================================== From tifei <@t> foxmail.com Thu Jul 31 10:44:26 2008 From: tifei <@t> foxmail.com (tf) Date: Thu Jul 31 10:44:43 2008 Subject: [Histonet] PSA_NCAM immunohistochemistry Message-ID: <200807312344210286206@foxmail.com> Dear All: Just wonder anyone have worked on PSA_NCAM IHC of frozen sections? How about 1:1000 mouse anti-PSA NCAM from millipore to stain 30 um brain sections? And, do you need to add triton into the PBS, to "pore" the membrane? I think PSA-NCAM is distributed on the cell membrane, thus PBS may work? Protocol: 1. Rehydration. PBS 2. Mouse-anti PSA-NCAM 1:1000 overnight + Blocking simultaneously. 3. 3* PBS 4. Goat-anti mouse conjugated with 488 fluroscein 5. 3 * PBS 6. DAPI 7. PBS wash 8. mount Any other comments on this? especially the time & concentration for first antibody? thx, 2008-07-31 tf From froyer <@t> bitstream.net Thu Jul 31 11:14:37 2008 From: froyer <@t> bitstream.net (Ford Royer) Date: Thu Jul 31 11:14:58 2008 Subject: [Histonet] Sakura V.I.P In-Reply-To: References: Message-ID: <009b01c8f328$87ca90a0$7701a80a@Ford> You did not mention the specific model number, but I believe that I can be of help with this. I have done it before with the "K" & "E" series processors. Contact me "off-List" for more information. ~ Ford Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL:? 612-839-1046 Phone:? 763-542-8725 Fax:? 763-546-4830 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall, Kimberly Sent: Thursday, July 31, 2008 10:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura V.I.P Hello all, I am wondering if anyone has had to change a Sakura Tissue-Tek Processor from counter top or side by side, to a up-right or stand alone. I have a very small lab and need to make room for a new processor and it will all fit if there is a way to change it. Any advise or info would be helpful. Kimberly Marshall H.T. (ASCP) Metroplex Hospital ============================================================================ == The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ============================================================================ == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Thu Jul 31 12:33:10 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Thu Jul 31 12:33:16 2008 Subject: [Histonet] Re: Alcian yellow Message-ID: Jenny (where?) asks: >>I wanted to get some feed back from anyone using Alcian yellow as opposed to Giemsa for staining Helicobacter pylori. Right now we are using Giemsa for detection of H. pylori, and are considering changing to Alcian yellow. Just wanted your opinion of what is most preferred by the pathologists.<< Alcian yellow is a dye that cannot be manufactured safely for the workers who make it, and it's now only made in countries that do not concern themselves about worker safety. For a substitute, go to Anatech's Web site (anatechltdusa.com). I have no connection with Anatech. I have no experience with any of these stains. Giemsa (better just to use Diff-Quik II or a generic substitute, or prepare toluidine blue yourself). In my experience the immunostain is much faster to read, but I don't think it's known to be more sensitive. If I have one Giemsa slide to read and a work load of 15 cases a day, Giemsa is fine. If I have ten gastric biopsies and a work load of 70 cases a day, then I want the immunostain. Bob Richmond Samurai Pathologist Knoxville TN From AFeatherstone <@t> KaleidaHealth.Org Thu Jul 31 12:50:30 2008 From: AFeatherstone <@t> KaleidaHealth.Org (Featherstone, Annette) Date: Thu Jul 31 12:50:40 2008 Subject: [Histonet] HPylori In-Reply-To: References: Message-ID: <16A5E67B2A1F714885DEDC2CB68DD6091D7B20@KALEXMB03.KaleidaHealth.org> Our Pathologists like the Warthin Starry Stain for Hpylori. Annette Featherstone, Kaleida Health -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, July 31, 2008 13:04 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 56, Issue 37 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Grossing of complex specimens by PAs and/or residents in pathology (Luke A Perkocha) 2. PSA_NCAM immunohistochemistry (tf) 3. Alcian Yellow (zodiac29@comcast.net) 4. Alcohol fixation and immuno (Martin, Erin) 5. Re: Processing of mouse hearts (Maxim_71@mail.ru) 6. Re: -86 freezers (Jane M Flinn) 7. Alcian Yelllow for H.pylori (zodiac29@comcast.net) 8. Re: Alcohol fixation and immuno (Patti Loykasek) 9. Susie Hargrove is out of the office. (SHargrove@urhcs.org) 10. RE: Fwd: [Histonet] Processing of mouse hearts (Monfils, Paul) 11. Please Unsubscribe (Neehar Bhatia) 12. Unsubscribe (Juan Gutierrez) 13. Re: Unsubscribe (Peter Carroll) 14. (no subject) (nealtaylor01@bellsouth.net) 15. Alcian Yellow (zodiac29@comcast.net) 16. Re: Alcian Yelllow for H.pylori (Gayle Callis) 17. RE: Unsubscribe (Tony Henwood) 18. RE: Alcian Yellow (Tony Henwood) 19. Looking for a Position (Steven Coakley) 20. Re: Alcohol fixation and immuno (Richard Cartun) 21. PSLIM Slide Labeling (Victor Tobias) 22. Basic staining questions (Keith Mc Quillan) 23. Sakura V.I.P (Marshall, Kimberly) 24. PSA_NCAM immunohistochemistry (tf) 25. RE: Sakura V.I.P (Ford Royer) ---------------------------------------------------------------------- Message: 1 Date: Wed, 30 Jul 2008 10:03:50 -0700 From: "Luke A Perkocha" Subject: [Histonet] Grossing of complex specimens by PAs and/or residents in pathology To: histonet@lists.utsouthwestern.edu Message-ID: <6.2.3.4.2.20080730092835.0204d458@exchange.ucsf.edu> Content-Type: text/plain; charset=us-ascii; format=flowed Greetings, The use of Pathology Assistants to do virtually all of the grossing of even complex pathology specimens, under the supervision of a pathologist is becoming commonplace in larger practices, and many are convinced it provides a more consistent, higher quality gross dissection and sampling, as long as the PAs are properly qualified, trained and supervised and have pathologist back-up. Conversely, in most pathology training programs, the residents do most or all of the grossing as part of their training (sometimes with the exception of small biopsies). However, this is counter-intuitive from a patient safety standpoint: i.e. your least experienced people, who are interrupted by other concurrent responsibilities and rotate in and out on a scheduled bases are doing the part of pathology which is most critical to patient safety and can't be replicated if done wrong. We are considering tweaking our program in some ways to improve patient safety in the gross room and reduce errors and would like to see what others do and think. The concept being tested is that more of the grossing of complex specimens should be done by PAs and experienced residents (not fresh trainees who are trying to learn all aspects of pathology and lab at the same time and are prone to errors) and the training of residents should also include aspects of directing a gross pathology lab (which requires knowing how to gross, but also other things), rather than just grossing alone. Could you help with the answers to these questions if you have a residency training program in pathology: * Do PAs gross complex specimens? * Are PAs involved in training residents or vice-versa? * What is the salary for PAs who gross complex specimens? * Do you have any other innovative ways to reduce gross room errors by residents in training or to teach gross pathology to same? Luke A. Perkocha, MD, MBA Associate Professor of Pathology and Dermatology Associate Director of Surgical Pathology UC San Francisco Helen Diller Family Comprehensive Cancer Center 1600 Divisadero Avenue, Box 1785 San Francisco, CA 94143-1785 office: 415 885-7254 cell: 415 509-6442 ------------------------------ Message: 2 Date: Thu, 31 Jul 2008 01:11:21 +0800 From: "tf" Subject: [Histonet] PSA_NCAM immunohistochemistry To: "histonet" Message-ID: <200807310111158852149@foxmail.com> Content-Type: text/plain; charset="gb2312" Dear All: Just wonder anyone have worked on PSA_NCAM IHC of frozen sections? How about 1:1000 mouse anti-PSA NCAM from millipore to stain 30 um brain sections? And, do you need to add triton into the PBS, to "pore" the membrane? I think PSA-NCAM is distributed on the cell membrane, thus PBS may work? Protocol: 1. Rehydration. PBS 2. Mouse-anti PSA-NCAM 1:1000 overnight + Blocking simultaneously. 3. 3* PBS 4. Goat-anti mouse conjugated with 488 fluroscein 5. 3 * PBS 6. DAPI 7. PBS wash 8. mount Any other comments on this? especially the time & concentration for first antibody? thx, 2008-07-31 tf ------------------------------ Message: 3 Date: Wed, 30 Jul 2008 17:16:10 +0000 From: zodiac29@comcast.net Subject: [Histonet] Alcian Yellow To: histonet@lists.utsouthwestern.edu Message-ID: <073020081716.1090.4890A1DA000B321A000004422215575474C7CD0C0E070B0196@comcast.net> Content-Type: text/plain Hello all, I wanted to get some feed back from anyone using Alcian yellow as opposed to Geimsa for staining H.Pylori. Right now we are using Gemisa for detection of H.Pylori, and are considering changing to Alcian Yellow. Just wanted your opinion of what is most preferred by the pathologists. Thanks in Advance Jenny ------------------------------ Message: 4 Date: Wed, 30 Jul 2008 10:31:51 -0700 From: "Martin, Erin" Subject: [Histonet] Alcohol fixation and immuno To: "histonet" Message-ID: Content-Type: text/plain; charset=iso-8859-1 Hi everyone, We occassionally get blocks from outside labs that use alcohol as the fixative. Our pathologist told me that our immunos look pretty bad when we handle these cases in the same manner as our routine FFPE cases. He is most concerned with Ki67 and K/L. I tried them with no antigen retrieval but got no staining at all. Any thoughts? We only do derm. Thanks, Erin ------------------------------ Message: 5 Date: Wed, 30 Jul 2008 22:17:18 +0400 From: Maxim_71@mail.ru Subject: Re: [Histonet] Processing of mouse hearts To: bturdi@gmail.com Cc: histonet@lists.utsouthwestern.edu Message-ID: <1253698354.20080730221718@mail.ru> Content-Type: text/plain; charset=us-ascii Subat: Here is my manual protocol for mouses tissues, that works very well for all mouse tissues (thickness no more than 3 mm): 1. Isopropanol 70% 30 min 2. Isopropanol 80% 30 min 3. Isopropanol 95% 30 min 4. Isopropanol 99% 30 min 5. Isopropanol:Mineral oil 5:1 50oC 60 min 6. Isopropanol:Mineral oil 2:1 50oC 60 min 7. Mineral oil 1.5 h (or overnight) 8. Paraffin 60oC 30 min 9. Paraffin 60oC 30 min 10. Paraffin 60oC 20 min 11. Paraffin 60oC 20 min. Advantage of isopropanol consists in that ethanol have "tide" effect and do tissues hard, but isopropanol (IPA) not. Mineral oil = paraffin with low molecular weight and the infiltration is better and gentle. Mineral oil is absolutely not toxic for personnel. Transitions between IPA, MO and paraffin are very gentle. Please let me know about results. If you need any more details please contact to me. Best wishes, Maxim Peshkov Russia, Taganrog. mailto:Maxim_71@mail.ru ------------------------------ Message: 6 Date: Wed, 30 Jul 2008 14:23:40 -0400 From: Jane M Flinn Subject: [Histonet] Re: -86 freezers To: sridley@gmu.edu Cc: histonet Message-ID: Content-Type: text/plain; charset="us-ascii" WE are interested in buying a 30 cu ft -86 upright freezer. Recommnendations appreciated. Jane "Life is short - make haste to be kind" Dr. Jane Flinn Director, Biopsychology Program George Mason University, 3F5 4400 University Dr. Fairfax, VA 22030 Phone: 703-993-4107 Fax: 703-993-1359 ----- Original Message ----- From: "Martin, Erin" Date: Wednesday, July 30, 2008 1:31 pm Subject: [Histonet] Alcohol fixation and immuno > Hi everyone, > > We occassionally get blocks from outside labs that use alcohol as > the fixative. Our pathologist told me that our immunos look > pretty bad when we handle these cases in the same manner as our > routine FFPE cases. He is most concerned with Ki67 and K/L. I > tried them with no antigen retrieval but got no staining at all. > Any thoughts? We only do derm. > > Thanks, > Erin > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 7 Date: Wed, 30 Jul 2008 18:33:44 +0000 From: zodiac29@comcast.net Subject: [Histonet] Alcian Yelllow for H.pylori To: histonet@lists.utsouthwestern.edu Message-ID: <073020081833.14431.4890B40800030A6B0000385F2215578674C7CD0C0E070B0196@comcast.net> Content-Type: text/plain Rosa and Ellen, Thank you for your quick responses : ) Does anyone happen to have the procedure for Alcian yellow for H.Pylori? Thanks again Jenny ------------------------------ Message: 8 Date: Wed, 30 Jul 2008 11:38:14 -0700 From: Patti Loykasek Subject: Re: [Histonet] Alcohol fixation and immuno To: "Martin, Erin" , histonet Message-ID: Content-Type: text/plain; charset="US-ASCII" HI Erin. The best situation would be you could speak with this client, and persuade them to use formalin, too. Let them know your IHC protocols are optimized for FFPE (probably H&E's would look better, too). If this doesn't work, obtain some alcohol fixed control material and optimize your IHC for this material, too. You will need to try a variety of pretreatments & titers. In essence you will have 2 protocols for your IHC - 1 for FFPE & 1 for alcohol fixed. What fun! Good luck with working all this out. Patti Loykasek BS, HTL, QIHC Clinical IHC Supervisor PhenoPath Laboratories Seattle, WA > Hi everyone, > > We occassionally get blocks from outside labs that use alcohol as the > fixative. Our pathologist told me that our immunos look pretty bad when we > handle these cases in the same manner as our routine FFPE cases. He is most > concerned with Ki67 and K/L. I tried them with no antigen retrieval but got > no staining at all. Any thoughts? We only do derm. > > Thanks, > Erin > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ------------------------------ Message: 9 Date: Wed, 30 Jul 2008 13:43:22 -0500 From: SHargrove@urhcs.org Subject: [Histonet] Susie Hargrove is out of the office. To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 07/30/2008 and will not return until 08/04/2008. I will respond to your message when I return. If immediate assistance is needed please call 3198. ------------------------------ Message: 10 Date: Wed, 30 Jul 2008 15:13:20 -0400 From: "Monfils, Paul" Subject: RE: Fwd: [Histonet] Processing of mouse hearts To: "subat turdi" , "Derek Papalegis" Cc: histonet@lists.utsouthwestern.edu Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835C43@LSRIEXCH1.lsmaster.lifespan.org> Content-Type: text/plain; charset="iso-8859-1" > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Derek Papalegis > Sent: Wednesday, July 30, 2008 12:52 PM > To: subat turdi > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: Fwd: [Histonet] Processing of mouse hearts > > This is a common problem with mouse tissue. The tissue is being over > processed. There are a few things that you can do to remedy this. One is > to have a dedicated processing schedule for each different tissue that > you process. Although this is ideal, it is often times impossible to > realistically do this. If your lab is processing a variety of different > mouse tissues at the same time, you will frequently encounter over > processed tissue. Although not ideal, a way to get good sections is to > rough into the blocks and before you put them on your cold tray, place > them in your heated water bath for a few seconds. This will rehydrate > the tissue enough so you can get good sections. If you do this often > enough, you will get a "feel" for how long you need to leave them in > your water bath. > > It seems like you have a few unnecessary steps in how you prepare these > hearts. Is there a specific reason why you use cold PBS to rinse the > blood out of the heart? Wouldn't formalin rinse the blood off just as > effectively as PBS while also starting the fixation process more > quickly? What is your reasoning for fixing at 4 degrees instead of at > room temp? Lowering the temp slows down the fixation process. The 30 > minute running water rinse after fixation is unnecessary as well. > > Feel free to contact me if you have additional questions. > Derek > > Derek Papalegis HT (ASCP) > Histotechnician > Division of Laboratory Animal Medicine > Tufts University > 136 Harrison Avenue > Boston, MA 02111 > phone: 617 636-2971 > fax: 617 636-8354 > > > > subat turdi wrote: > > Folks, I appreciate your responses very much. > > This is the protocol I use. Please give me some advice what has gone wrong. > > Animals weighed and anesthetized w/ ketamine-xylazine combo.Chest cavity > > opened quickly and the heart was exposed. IVC was found and KCl was injected > > iv to arrest the heart was in diastole.The heart was taken out fast and > > placed in a cold PBS to wash out the excess blood. (Perfusion w/ PFA through > > aorta is optional) .The heart was cut into several ~2 mm thick doughnuts > > with special interest on the ventriculum then put into 4% PFA solution for > > 24h at 4 degrees.Then the tissue was rinsed with running water for > > 30min.Then subjected to graded alcohols. > > > > 70% 30min > > > > 95% 20min X2 > > > > 100% 20minX2 > > > > Xylene 20min > > > > Xylene 20 min > > Infiltrate in paraplast: > > Station 1: 45min > > Station 2 : 45min > > Embedding with paraplast > > > > My major problem is that a lot of times I get dry blocks. The ribbons i get > > often comes without the tissue and breaks into shards. > > > > Subat > > > > > > On 7/30/08, Gayle Callis wrote: > > > >> If you tell us how YOU do it, then we can help modify your protocol. > >> ----- Original Message ----- From: "subat turdi" > >> To: > >> Sent: Tuesday, July 29, 2008 9:31 PM > >> Subject: [Histonet] Processing of mouse hearts > >> > >> > >> Dear all, > >> > >>> Does anyone have a detailed protocol for processing mouse hearts for > >>> paraffin slides? I am new to this technique and having various troubles in > >>> getting good histology. We don't have a automatic processer and all is > >>> manual. Thanks in advance. > >>> > >>> Subat Turdi > >>> > >>> School of Pharmacy > >>> University of Wyoming > >>> Laramie, Wyoming > >>> _______________________________________________ > >>> Histonet mailing list > >>> Histonet@lists.utsouthwestern.edu > >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >>> > >>> > > >> > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 11 Date: Wed, 30 Jul 2008 14:23:48 -0500 From: Neehar Bhatia Subject: [Histonet] Please Unsubscribe To: "histonet@lists.utsouthwestern.edu" Message-ID: <20080730142348531.00000000760@derm-7W5SGF1> Content-Type: text/plain; charset=us-ascii Please unsubscribe Thanks Neehar Bhatia Assistant Scientist Department of Dermatology 1300 University Avenue, Rm 445 Madison WI 53706 Ph. 608.263.7144 Fax. 608.263.5223 ------------------------------ Message: 12 Date: Wed, 30 Jul 2008 14:36:42 -0500 From: "Juan Gutierrez" Subject: [Histonet] Unsubscribe To: Message-ID: <46B933DABE5D477A9221C2DFDD634909@precisionpath.lcl> Content-Type: text/plain; charset="us-ascii" Please unsubscribe me from the list. Thank you, Juan C. Gutierrez, HT(ASCP) ------------------------------ Message: 13 Date: Wed, 30 Jul 2008 15:46:33 -0400 From: Peter Carroll Subject: Re: [Histonet] Unsubscribe Cc: histonet@lists.utsouthwestern.edu Message-ID: <4890C519.6000307@umdnj.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Just for the record... do all of you "unsubscribers" know that you can click the link that's at the bottom of every email from this list and unsubscribe yourself? Just a tip! http://lists.utsouthwestern.edu/mailman/listinfo/histonet Juan Gutierrez wrote: > Please unsubscribe me from the list. > > > > Thank you, > > > > Juan C. Gutierrez, HT(ASCP) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ Message: 14 Date: Wed, 30 Jul 2008 20:23:46 +0000 From: nealtaylor01@bellsouth.net Subject: [Histonet] (no subject) To: Histonet@lists.utsouthwestern.edu Message-ID: <073020082023.22722.4890CDD20006E9C1000058C222216125569B0A02D2089B9A019C04040A0DBFCECF9D0104970E9B040E0A02@att.net> Content-Type: text/plain Please unsubscribe - thanks ------------------------------ Message: 15 Date: Wed, 30 Jul 2008 20:46:14 +0000 From: zodiac29@comcast.net Subject: [Histonet] Alcian Yellow To: histonet@lists.utsouthwestern.edu Message-ID: <073020082046.2974.4890D3160001B76500000B9E2216551406C7CD0C0E070B0196@comcast.net> Content-Type: text/plain Thank you everyone for your swift replies concerning the Alcian Yellow stain, it is much appreciated : ) Jenny ------------------------------ Message: 16 Date: Wed, 30 Jul 2008 15:04:45 -0600 From: "Gayle Callis" Subject: Re: [Histonet] Alcian Yelllow for H.pylori To: , Message-ID: <001c01c8f287$e4f41f90$6401a8c0@Sunney> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original You can buy the staining kits from Master Tech and probably Newcomer's or Poly Scientific certainly easier than making it up, plus kits come with directions. I belive staining protocols have been posted on Histonet several times - you can check Histonet Archives Gayle M. Callis HTL/HT/MT(ASCP) ----- Original Message ----- From: To: Sent: Wednesday, July 30, 2008 12:33 PM Subject: [Histonet] Alcian Yelllow for H.pylori > > Rosa and Ellen, Thank you for your quick responses : ) > Does anyone happen to have the procedure for Alcian yellow for H.Pylori? > > Thanks again > Jenny > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Thu, 31 Jul 2008 09:13:46 +1000 From: "Tony Henwood" Subject: RE: [Histonet] Unsubscribe To: "Peter Carroll" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="us-ascii" Yep Nice and easy BUT I WOULD NEVER CONSIDER IT Histonet is too good! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Peter Carroll Sent: Thursday, 31 July 2008 5:47 AM Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Unsubscribe Just for the record... do all of you "unsubscribers" know that you can click the link that's at the bottom of every email from this list and unsubscribe yourself? Just a tip! http://lists.utsouthwestern.edu/mailman/listinfo/histonet Juan Gutierrez wrote: > Please unsubscribe me from the list. > > > > Thank you, > > > > Juan C. Gutierrez, HT(ASCP) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 18 Date: Thu, 31 Jul 2008 09:41:31 +1000 From: "Tony Henwood" Subject: RE: [Histonet] Alcian Yellow To: , Message-ID: Content-Type: text/plain; charset="us-ascii" I never saw any! Why? Please use "reply to all" so that everyone on the list can see your contributions. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of zodiac29@comcast.net Sent: Thursday, 31 July 2008 6:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Alcian Yellow Thank you everyone for your swift replies concerning the Alcian Yellow stain, it is much appreciated : ) Jenny _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 19 Date: Wed, 30 Jul 2008 18:38:51 -0700 (PDT) From: Steven Coakley Subject: [Histonet] Looking for a Position To: histonet@lists.utsouthwestern.edu Message-ID: <62903.6139.qm@web38207.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I'm looking for a full time?HT position im the Madison, WI area.? Any information, especially research facilities that employ HT's would be appreciated. ? Thanks, ? ? ------------------------------ Message: 20 Date: Thu, 31 Jul 2008 10:45:59 -0400 From: "Richard Cartun" Subject: Re: [Histonet] Alcohol fixation and immuno To: "histonet" , "Erin Martin" Message-ID: <489197E7020000770000484A@gwmail6.harthosp.org> Content-Type: text/plain; charset=US-ASCII Are they using pure alcohol for fixation? I do IHC for a dermpath lab that uses alcoholic formalin and their tissue stains very well. The only difference is we have had to cut-back on our antigen retrieval for certain proteins. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Martin, Erin" 07/30/08 1:31 PM >>> Hi everyone, We occassionally get blocks from outside labs that use alcohol as the fixative. Our pathologist told me that our immunos look pretty bad when we handle these cases in the same manner as our routine FFPE cases. He is most concerned with Ki67 and K/L. I tried them with no antigen retrieval but got no staining at all. Any thoughts? We only do derm. Thanks, Erin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Thu, 31 Jul 2008 07:55:07 -0700 From: Victor Tobias Subject: [Histonet] PSLIM Slide Labeling To: Histonet Message-ID: <4891D24B.1060800@pathology.washington.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Does anyone have the Pslim slide labeler from AccuPlace. http://www.accuplace.com/PSLIM.asp It sounds really nice for our work environment, but I'd like some real world feedback. Victor -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. ------------------------------ Message: 22 Date: Thu, 31 Jul 2008 16:23:06 +0100 From: "Keith Mc Quillan" Subject: [Histonet] Basic staining questions To: histonet@lists.utsouthwestern.edu Message-ID: <94847270807310823p72b56af7vd1481f58a1641edb@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi, I am pretty new to staining (as in i have only ever tried it once) and would appreciate any help you can give me with regard to processing my tissue and staining. I will be processing mouse brains in the coming weeks with the intention of staining for amyloid, and want to check about the correct method for fixing this tissue. In our lab, we have always snap frozen tissue in OCT directly after taking it, no sucrose gradients or aldehydes etc.However, i have noticed from reading the literature that most labs seem to fix the tissue in 4% paraformaldehyde before fixing in paraffin. Does it make any difference as to which protocol is used? I am reluctant to change unless i have good reason to. I am planning on perfusing the animals with PBS before taking half the brain for immunohistochemistry (in OCT) and the other half for ELISA/RNA etc and so can't perfuse with PFA. My other question relates to the staining for amyloid beta itself. I have noticed that a number of protocols incorporate a step to treat the sections with 70% formic acid prior to staining. Is this neccessary for the staining of amyloid, and if so, what advantages does it offer. Any help anyone can provide me with would be greatly appreciated Thanks Keith Mc Quillan Trinity College Dublin ------------------------------ Message: 23 Date: Thu, 31 Jul 2008 11:34:16 -0400 From: "Marshall, Kimberly" Subject: [Histonet] Sakura V.I.P To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=iso-8859-1 Hello all, I am wondering if anyone has had to change a Sakura Tissue-Tek Processor from counter top or side by side, to a up-right or stand alone. I have a very small lab and need to make room for a new processor and it will all fit if there is a way to change it. Any advise or info would be helpful. Kimberly Marshall H.T. (ASCP) Metroplex Hospital ============================================================================== The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ============================================================================== ------------------------------ Message: 24 Date: Thu, 31 Jul 2008 23:44:26 +0800 From: "tf" Subject: [Histonet] PSA_NCAM immunohistochemistry To: "histonet" Message-ID: <200807312344210286206@foxmail.com> Content-Type: text/plain; charset="gb2312" Dear All: Just wonder anyone have worked on PSA_NCAM IHC of frozen sections? How about 1:1000 mouse anti-PSA NCAM from millipore to stain 30 um brain sections? And, do you need to add triton into the PBS, to "pore" the membrane? I think PSA-NCAM is distributed on the cell membrane, thus PBS may work? Protocol: 1. Rehydration. PBS 2. Mouse-anti PSA-NCAM 1:1000 overnight + Blocking simultaneously. 3. 3* PBS 4. Goat-anti mouse conjugated with 488 fluroscein 5. 3 * PBS 6. DAPI 7. PBS wash 8. mount Any other comments on this? especially the time & concentration for first antibody? thx, 2008-07-31 tf ------------------------------ Message: 25 Date: Thu, 31 Jul 2008 11:14:37 -0500 From: "Ford Royer" Subject: RE: [Histonet] Sakura V.I.P To: "'Marshall, Kimberly'" , Message-ID: <009b01c8f328$87ca90a0$7701a80a@Ford> Content-Type: text/plain; charset="iso-8859-1" You did not mention the specific model number, but I believe that I can be of help with this. I have done it before with the "K" & "E" series processors. Contact me "off-List" for more information. ~ Ford Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL:? 612-839-1046 Phone:? 763-542-8725 Fax:? 763-546-4830 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall, Kimberly Sent: Thursday, July 31, 2008 10:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura V.I.P Hello all, I am wondering if anyone has had to change a Sakura Tissue-Tek Processor from counter top or side by side, to a up-right or stand alone. I have a very small lab and need to make room for a new processor and it will all fit if there is a way to change it. Any advise or info would be helpful. Kimberly Marshall H.T. (ASCP) Metroplex Hospital ============================================================================ == The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ============================================================================ == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 56, Issue 37 **************************************** 2007 Best Places to Work Finalist Visit our careers page at www.kaleidahealth.org/careers CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From cbass <@t> wfubmc.edu Thu Jul 31 13:14:57 2008 From: cbass <@t> wfubmc.edu (Caroline Bass) Date: Thu Jul 31 13:15:09 2008 Subject: [Histonet] BSA in immunhistochemistry Message-ID: Hey Guys, I am trying to set up EGFP IHC in rat brain and just ran the first set of tissue through. We just guessed at the primary and secondary antibody concentrations, and we used an ABC kit with vector-sg substrate. The sections developed very quickly with lots of background. My guess is to decrease the primary concentration and maybe block longer. However, we?ve encountered a lot of IHC protocols that use BSA during blocking. I?m hesitant due to the expense though. Any suggestions as to whether this will help and if so is there a cheap source of BSA that I can use? Thanks, Caroline Bass From mabosso <@t> unipathllc.com Thu Jul 31 13:17:45 2008 From: mabosso <@t> unipathllc.com (Mary Abosso) Date: Thu Jul 31 13:17:52 2008 Subject: [Histonet] Research Only antibodies Message-ID: <43A451981FF6634795BE83B1B5494D631364E3@exchange.unipathllc.corp> All - A recent teleconference presenter stated that RUO antibodies are manufactured with no required quality standards, and that in using them, they can not be billed to the patient. I am just inquiring as to what others in diagnostic human pathology are doing. Additionally, there is a CAP standard associated with the use of ASR and RUO antibodies and I would also like to hear how others are handling this. Mary Abosso UniPath LLC Denver, CO From liz <@t> premierlab.com Thu Jul 31 13:19:17 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Jul 31 13:19:22 2008 Subject: [Histonet] cross polarization Message-ID: Is anyone out there familar with cross polarization. Can it be performed on a standard microscope? thanks Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 From sheila_adey <@t> hotmail.com Thu Jul 31 13:29:27 2008 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Thu Jul 31 13:29:32 2008 Subject: [Histonet] CAP Inspection last Friday. In-Reply-To: References: <001201c8f244$582bc2e0$3d02a8c0@plab.local> Message-ID: We are awaiting ours also. Our window just started. Always nice to meet new people.Sheila Adey HT MLTPort Huron HospitalMichigan> Date: Wed, 30 Jul 2008 10:18:23 -0400> From: Lynne.Bell@hitchcock.org> To: cmiller@physlab.com; histonet@lists.utsouthwestern.edu> Subject: RE: [Histonet] CAP Inspection last Friday.> CC: > > Congratulations on surviving your CAP inspection. We are anxiously> awaiting ours. Our three month window ends September 30. As each day> goes past 9:00 and inspectors are not banging on the door, we all take a> deep breath and then do it all over again the next day!!> > Lynne Bell, HT (ASCP)> Lead Histologist> Central Vermont Hospital> P. O. Box 547> Barre, VT 05641> 802-371-4923> > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ From HornHV <@t> archildrens.org Thu Jul 31 13:47:11 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Jul 31 13:47:08 2008 Subject: [Histonet] Research Only antibodies In-Reply-To: <43A451981FF6634795BE83B1B5494D631364E3@exchange.unipathllc.corp> References: <43A451981FF6634795BE83B1B5494D631364E3@exchange.unipathllc.corp> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82D61@EMAIL.archildrens.org> When we used RUO antibodies we did not bill for them. Now all of our antibodies are IVD. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mary Abosso Sent: Thursday, July 31, 2008 1:18 PM To: Histonet Subject: [Histonet] Research Only antibodies All - A recent teleconference presenter stated that RUO antibodies are manufactured with no required quality standards, and that in using them, they can not be billed to the patient. I am just inquiring as to what others in diagnostic human pathology are doing. Additionally, there is a CAP standard associated with the use of ASR and RUO antibodies and I would also like to hear how others are handling this. Mary Abosso UniPath LLC Denver, CO _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From rjbuesa <@t> yahoo.com Thu Jul 31 13:48:52 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jul 31 13:48:57 2008 Subject: [Histonet] cross polarization In-Reply-To: Message-ID: <135129.55038.qm@web65708.mail.ac4.yahoo.com> Yes, it can be done. You just need two polarizing filters, one to be placed at the exit of the light source (before the light enters?the microscope condenser) and the second over the slide (or inside one ocular). You will make the field dark by crossing the two filters and later you place the object and will determine if there was a change in the field characteristics. Ren? J. --- On Thu, 7/31/08, Liz Chlipala wrote: From: Liz Chlipala Subject: [Histonet] cross polarization To: histonet@lists.utsouthwestern.edu Date: Thursday, July 31, 2008, 2:19 PM Is anyone out there familar with cross polarization. Can it be performed on a standard microscope? thanks Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu Jul 31 13:52:07 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Jul 31 13:52:13 2008 Subject: [Histonet] cross polarization In-Reply-To: <135129.55038.qm@web65708.mail.ac4.yahoo.com> References: <135129.55038.qm@web65708.mail.ac4.yahoo.com> Message-ID: Rene I thinks that just regular polarized light, I believe that cross polarization is different from that. The filters are twisted to 90 degrees or something like that. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 ________________________________ From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Thursday, July 31, 2008 12:49 PM To: histonet@lists.utsouthwestern.edu; Liz Chlipala Subject: Re: [Histonet] cross polarization Yes, it can be done. You just need two polarizing filters, one to be placed at the exit of the light source (before the light enters the microscope condenser) and the second over the slide (or inside one ocular). You will make the field dark by crossing the two filters and later you place the object and will determine if there was a change in the field characteristics. Ren? J. --- On Thu, 7/31/08, Liz Chlipala wrote: From: Liz Chlipala Subject: [Histonet] cross polarization To: histonet@lists.utsouthwestern.edu Date: Thursday, July 31, 2008, 2:19 PM Is anyone out there familar with cross polarization. Can it be performed on a standard microscope? thanks Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ArtimK <@t> slhn.org Thu Jul 31 14:05:56 2008 From: ArtimK <@t> slhn.org (Artim, Kimberly) Date: Thu Jul 31 14:06:01 2008 Subject: [Histonet] Number of blocks per case Message-ID: <397958B23C3E4D43AB50099AE6313C382FEF0D@slhmailsvr.slhn.org> Could anyone share with me the average # of blocks per case at their institution. I would like figures from hospitals comparable to ours. We are a city hospital with approximately 25,000 routine surgical cases per year. We receive a wide range of specimens. 25% are derm, 25% are GI biopsies and the rest are complex specimens. Thank you in advance for your help. Kim Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From PMonfils <@t> Lifespan.org Thu Jul 31 14:10:58 2008 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Jul 31 14:11:04 2008 Subject: [Histonet] cross polarization In-Reply-To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835C44@LSRIEXCH1.lsmaster.lifespan.org> Cross polarization is "regular" polarization. You place one polarizer between the light source and the slide, the other between the slide and your eye or camera. To produce the polarization effect you rotate either of the polarizers until its so-called "optical slits" are oriented perpendicular to those of the other polarizer. You know when this orientation is achieved because the direct light from the light source is almost entirely extinguished, creating a dark field. If there are any birefringent materials present, they will glow brightly against the dark background. From algranth <@t> u.arizona.edu Thu Jul 31 14:55:32 2008 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Thu Jul 31 14:55:33 2008 Subject: [Histonet] PSLIM Slide Labeling In-Reply-To: <4891D24B.1060800@pathology.washington.edu> References: <4891D24B.1060800@pathology.washington.edu> Message-ID: <6.2.3.4.1.20080731125339.01f60930@algranth.inbox.email.arizona.edu> I am looking at this slide labeler. In fact we may be close to purchasing the thing. The size and price are both what attracted me but I was wondering what the users (if any) think of the instrument. Andi Grantham At 07:55 AM 7/31/2008, Victor Tobias wrote: >Does anyone have the Pslim slide labeler from AccuPlace. >http://www.accuplace.com/PSLIM.asp It sounds really nice for our >work environment, but I'd like some real world feedback. > >Victor > >-- >Victor Tobias >Clinical Applications Analyst >University of Washington Medical Center >Dept of Pathology Room BB220 >1959 NE Pacific >Seattle, WA 98195 >victor@pathology.washington.edu >206-598-2792 >206-598-7659 Fax >================================================= >Privileged, confidential or patient identifiable information may be >contained in this message. This information is meant only for the >use of the intended recipients. If you are not the intended >recipient, or if the message has been addressed to you in error, do >not read, disclose, reproduce, distribute, disseminate or otherwise >use this transmission. Instead, please notify the sender by reply >e-mail, and then destroy all copies of the message and any attachments. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From JGREWE <@t> OhioHealth.com Thu Jul 31 15:02:36 2008 From: JGREWE <@t> OhioHealth.com (JGREWE@OhioHealth.com) Date: Thu Jul 31 15:02:45 2008 Subject: [Histonet] Jacquelyn Grewe/Staff/OhioHealth is out of the office . Message-ID: I will be out of the office starting 07/31/2008 and will not return until 08/04/2008. From contact <@t> excaliburpathology.com Thu Jul 31 15:05:03 2008 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Thu Jul 31 15:05:10 2008 Subject: [Histonet] PSLIM slide labelling Message-ID: <295787.11701.qm@web50102.mail.re2.yahoo.com> I am also looking at this and budgeting for possible purchase early next year. Would also like to hear from current users. ?Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 631 N. Broadway Ave. Moore, OK 73160 405-570-6679 cell 405-759-3953 lab contact@excaliburpathology.com www.excaliburpathology.com From amylee779 <@t> yahoo.com Thu Jul 31 16:12:20 2008 From: amylee779 <@t> yahoo.com (Amy Lee) Date: Thu Jul 31 16:12:24 2008 Subject: [Histonet] CD24 and CD9 antibodies Message-ID: <769549.47343.qm@web38008.mail.mud.yahoo.com> Hello histonetters, ? I am looking for good CD9 and CD24 antibodies that react with human but not mouse tissues. I checked many companies and they can only tell me that "they are not tested on other speices other than human.." ? Could anybody recommend?one you used before, for paraffin or frozen tissue? ? Thanks, Amy From dellav <@t> musc.edu Thu Jul 31 16:31:01 2008 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Thu Jul 31 16:28:49 2008 Subject: [Histonet] RE: BSA in immunhistochemistry In-Reply-To: References: Message-ID: You don't mention if you are doing a biotin block. Human brain has lots of biotin. Rat brain may also but I don't work with animal tissues however I'm certain others here can advise you on that issue. As I recall, the ABC kits do contain a protein block but do not contain a biotin block. I'm guessing this is what is creating the background you describe however don't underestimate the importance antibody dilution plays. If you know the protein concentration of your primary antibody, start with a dilution that yields 5-10 micrograms of protein as a dilution starting point and fine tune from there. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Caroline Bass Sent: Thursday, July 31, 2008 2:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BSA in immunhistochemistry Hey Guys, I am trying to set up EGFP IHC in rat brain and just ran the first set of tissue through. We just guessed at the primary and secondary antibody concentrations, and we used an ABC kit with vector-sg substrate. The sections developed very quickly with lots of background. My guess is to decrease the primary concentration and maybe block longer. However, we?ve encountered a lot of IHC protocols that use BSA during blocking. I?m hesitant due to the expense though. Any suggestions as to whether this will help and if so is there a cheap source of BSA that I can use? Thanks, Caroline Bass _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Annette.Fletcher <@t> providence.org Thu Jul 31 16:39:54 2008 From: Annette.Fletcher <@t> providence.org (Fletcher, Annette M) Date: Thu Jul 31 16:40:00 2008 Subject: [Histonet] Pathology Transcription Message-ID: <284596E350CB0743BB500B18475E7A0BF46159@wn1223.or.providence.org> Hi Histonetters, I am with Providence Health and Services Lab/Pathology. We are in Portland, OR and are seeking a strong Transcriptionist for our Pathology Lab. I don't wish to use a Recruiter or Agency for this position. Does anyone know if there is a good way to reach Pathology Transcriptionists who don't mind constant interruptions in a livewire environment such as ours? Thank you, Annette M Fletcher Senior Recruiter Providence Health & Services, Employment 1235 NE 47th Avenue - Suite 200 Portland, OR 97213 T: 503-215-5840 F: 503-215-4770 Annette.fletcher@providence.org DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From jdhisto <@t> yahoo.com Thu Jul 31 18:18:42 2008 From: jdhisto <@t> yahoo.com (JD) Date: Thu Jul 31 18:18:51 2008 Subject: [Histonet] Billing and coding Message-ID: Hello Everyone, I have an individual looking for a billing and/or coding position. She is very bright, top of her class and very ambitious. She is searching for a position in the "Coastal Bend" (Corpus Christi, Tx) area. She is relocating and would love to see what options she has in the laboratory setting. Thanks in advance for any and all feedback. I sure would hate to see great potential slip by. Sincerely, JDhisto From RSRICHMOND <@t> aol.com Thu Jul 31 19:06:33 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Thu Jul 31 19:06:38 2008 Subject: [Histonet] Re: cross polarization Message-ID: Liz Chlipala asks >>Is anyone out there familar with cross polarization? Can it be performed on a standard microscope?<< Somewhere in the Hospital Administrator's Handy-Dandy Guide to Making Life Difficult for the Pathologist (after more than 40 years as a pathologist, I remain convinced that there is such a book, probably with leather bosses and a padlock on it like a grimoire in a computer game, though I confess I've never seen a copy), and it says a pathologist doesn't need polarization. I've done a lot of polarization microscopy with broken sunglasses and bits of scrap polaroid, but it really needs to be built into the microscope, and it needs to include a full wave plate (first order plate, gout slider, or what have you). There's even a CPT code for doing polarization microscopy, though I'm not sure you're supposed to use it for tissue sections. I had polarization on my Dad's 1923 Leitz brass tube monocular (he was a pathologist too) when I was 11 years old and looking at mineral specimens, and it burns me up that my locum tenens clients can't have it now. Makes me wish I'd become a radiologist! Bob Richmond Samurai Pathologist Knoxville TN From Instaken <@t> aol.com Thu Jul 31 21:29:27 2008 From: Instaken <@t> aol.com (Instaken@aol.com) Date: Thu Jul 31 21:29:43 2008 Subject: [Histonet] Dictation systems Message-ID: Try Quickscribe. Just Google it. **************Get fantasy football with free live scoring. Sign up for FanHouse Fantasy Football today. (http://www.fanhouse.com/fantasyaffair?ncid=aolspr00050000000020) From RBARNHART <@t> summithealth.org Thu Jul 24 07:36:16 2008 From: RBARNHART <@t> summithealth.org (Rebecca Barnhart) Date: Tue Aug 5 07:45:23 2008 Subject: [Histonet] HT exam Message-ID: <48883F0002000057000026B6@carrierpigeon.SummitHealth.local> I have a coworker that has an associates degree in Medical Office Administration. She has been trained for Histology and has worked in the field for two and a half years. Is she eligible for to sit for the ASCP HT exam? Thank you Becky