[Histonet] plastic sectioning for light microscopy-HELP!!

[Danielle Crippen]

Dear Histo-experts,

 

This is a first for me as I have only done ultrathin sectioning for EM.


 

I need 0.5-1um serial sections for light microscopy.  My main problem at
this point is transferring the ribbons to a glass (plus) slide.  No
matter how I try to attempt the transfer, the ribbon comes apart and/or
sections are lost or badly wrinkled.  Any and all suggestions in this
area are very welcome!!

 

Additionally...once I actually get the sections onto the slide (which
feels like it might be cause for a celebration at this point!!)...what
are the most recommended H&E staining methods (these samples are
embedded in EPON)?

 

Again...I am a complete novice at plastic sectioning for light
microscopy...so any suggestions are welcome...even the most trivial:-)

 

A thousand thanks in advance!!

 

 

 

danielle