[Histonet] Counterstain on Ventana stainers

Thomas Pier tp2 <@t> medicine.wisc.edu
Thu Jan 10 10:53:24 CST 2008


I use Biocare's CAT hematoxylin mixed 50/50 with distilled water for one minute followed by a TBS-T rinse on my Lab Vision Autostainer 360.  It seems to do a pretty good job.

Tom Pier

>>> "Drew Sally A." <SDrew <@t> uwhealth.org> 01/10/08 10:29 AM >>>
Is anyone using a non-Ventana hematoxylin counterstain on their
immunostainers?  If so, what works best for you in terms of intensity
and stability.  Just looking at a bit of cost-cutting...
Thank you for your time!

Sally Ann Drew, MT(ASCP)
IHC/ISH Laboratory
University of Wisconsin Hosp. & Clinics
Madison, WI 53792
(608)265-6596

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