[Histonet] Brdu

[Igor Deyneko]

Dear Histonetters!
I have a question about Brdu. I have never used it. My questions are as
follows:
1) How should the tissues be processed , paraffin-embedded or frozen???
(Will be used with xenograft tumors)
2) If there are kits, which have you found give better results with minimal
non-specific staining
3) If not , is there a general protocol for this type of procedure?

Thank you for any help.

Sincerely,
Igor Deyneko
Infinity Pharmaceuticals
Cambridge, MA