From Rcartun <@t> harthosp.org Wed Jan 2 09:46:02 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Jan 2 09:46:36 2008 Subject: [Histonet] Batonella quintana Message-ID: <477B6B6A0200007700009F20@gwmail4.harthosp.org> Happy New Year to everyone. Does anyone have a case of Bacillary Angiomatosis with Bartonella quintana (confirmed)? I need 5 unstained slides for an antibody validation study. Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From schaundrawalton <@t> yahoo.com Wed Jan 2 10:12:01 2008 From: schaundrawalton <@t> yahoo.com (Schaundra Walton) Date: Wed Jan 2 10:12:24 2008 Subject: [Histonet] Cytology Question Message-ID: <239303.3495.qm@web58911.mail.re1.yahoo.com> Hey Histonetters! I'm posting a question for a collegue of mine. She works in cytolgoy. She would like to know how other people handle unlabeled/mislabeled PAP specimens. What is your policy? She posted on a cyto forum, but has not had any luck getting responses. I figured since some histo techs also do cytology prep someone here could help her. Thanks in advance. Schaundra Walton BS HTL(ASCP) Swedish American Hospital 1401 E. State St. Rockford, IL 61104 --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From JCollins <@t> palmbeachpath.com Wed Jan 2 10:23:33 2008 From: JCollins <@t> palmbeachpath.com (Judy Collins) Date: Wed Jan 2 10:23:55 2008 Subject: [Histonet] Cytology Question Message-ID: After a courtesy phone call to advise them of the problem, we return all mislabeled/unlabeled specimens to the clinician for proper identification. Judy Collins Palm Beach Pathology 2013 Ponce De Leon Avenue West Palm Beach, FL 33407 From TMcNemar <@t> lmhealth.org Wed Jan 2 10:36:31 2008 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Jan 2 10:35:26 2008 Subject: [Histonet] Cytology Question In-Reply-To: <239303.3495.qm@web58911.mail.re1.yahoo.com> Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F507@lmhsmail.lmhealth.org> We call the office and have someone come and label it. They are pretty good about coming. If no one came, we'd send it back to them. We also have a form that the person labeling it has to fill out and sign stating that they are certain of the identity and are accepting responsibility for the labeling. Yeah, they don't like that form very much! Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Schaundra Walton Sent: Wednesday, January 02, 2008 11:12 AM To: Histonet Subject: [Histonet] Cytology Question Hey Histonetters! I'm posting a question for a collegue of mine. She works in cytolgoy. She would like to know how other people handle unlabeled/mislabeled PAP specimens. What is your policy? She posted on a cyto forum, but has not had any luck getting responses. I figured since some histo techs also do cytology prep someone here could help her. Thanks in advance. Schaundra Walton BS HTL(ASCP) Swedish American Hospital 1401 E. State St. Rockford, IL 61104 --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bill501 <@t> mindspring.com Wed Jan 2 11:11:29 2008 From: bill501 <@t> mindspring.com (Bill) Date: Wed Jan 2 11:11:54 2008 Subject: [Histonet] Cytology Question In-Reply-To: References: Message-ID: At 11:23 AM -0500 1/2/08, Judy Collins wrote: >After a courtesy phone call to advise them of the problem, we return all >mislabeled/unlabeled specimens to the clinician for proper >identification. That is what we did. We do not do PAPs anymore, the regulatory BS was too onerous and cost effectiveness way in the red. -- ______________ Bill Blank, MD Heartland Lab From Ronald.Houston <@t> nationwidechildrens.org Wed Jan 2 11:12:56 2008 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Wed Jan 2 11:13:34 2008 Subject: [Histonet] BAL specimens Message-ID: <979FF5962E234F45B06CF0DB7C1AABB2144AC73E@chi2k3ms01.columbuschildrens.net> Is anyone having difficulty with cells staying on the slide during GMS staining? There is no problem with AFB, Gram, Oil Red O, Giemsa. This is only something that has become troublesome within the last couple of months. Our hematology lab insists they have not changed any part of the cytospin preparation, although some of the preps look suspicious for poor preparation with uneven cellular/exudate distribution on the slide. Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 Ronald.Houston@NationwideChildrens.org Columbus Children's Hospital is now Nationwide Children's Hospital www.NationwideChildrens.org From mpence <@t> grhs.net Wed Jan 2 11:28:07 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Jan 2 11:28:38 2008 Subject: [Histonet] Cytology Question In-Reply-To: <239303.3495.qm@web58911.mail.re1.yahoo.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C842@IS-E2K3.grhs.net> Since the specimen is a recollectable specimen the office is contacted and the specimen is rejected and logged in as rejected specimen - office contacted. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Schaundra Walton Sent: Wednesday, January 02, 2008 10:12 AM To: Histonet Subject: [Histonet] Cytology Question Hey Histonetters! I'm posting a question for a collegue of mine. She works in cytolgoy. She would like to know how other people handle unlabeled/mislabeled PAP specimens. What is your policy? She posted on a cyto forum, but has not had any luck getting responses. I figured since some histo techs also do cytology prep someone here could help her. Thanks in advance. Schaundra Walton BS HTL(ASCP) Swedish American Hospital 1401 E. State St. Rockford, IL 61104 --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmiller <@t> physlab.com Wed Jan 2 11:42:26 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Wed Jan 2 11:42:35 2008 Subject: [Histonet] Cytology Question In-Reply-To: <239303.3495.qm@web58911.mail.re1.yahoo.com> References: <239303.3495.qm@web58911.mail.re1.yahoo.com> Message-ID: <000301c84d66$d6c9a000$3d02a8c0@plab.local> We send the specimen back to the doctor's office. We add a neon yellow sticker to the front of the bag to alert that it is unlabelled. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Schaundra Walton Sent: Wednesday, January 02, 2008 10:12 AM To: Histonet Subject: [Histonet] Cytology Question Hey Histonetters! I'm posting a question for a collegue of mine. She works in cytolgoy. She would like to know how other people handle unlabeled/mislabeled PAP specimens. What is your policy? She posted on a cyto forum, but has not had any luck getting responses. I figured since some histo techs also do cytology prep someone here could help her. Thanks in advance. Schaundra Walton BS HTL(ASCP) Swedish American Hospital 1401 E. State St. Rockford, IL 61104 --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From mcauliff <@t> umdnj.edu Wed Jan 2 12:33:34 2008 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Jan 2 12:34:09 2008 Subject: [Histonet] mitachrondrial disease In-Reply-To: <005201c84454$025118f0$0202a8c0@ihctechq9h2qof> References: <005201c84454$025118f0$0202a8c0@ihctechq9h2qof> Message-ID: <477BD8FE.3090201@umdnj.edu> Hi Patsy: There is a good review of mitochondrial diseases in The Lancet, vol 368, p. 70, 1 July 2006. Perhaps that will help you decide on how to evaluate the tissue. Geoff pruegg@ihctech.net wrote: > Can someone recommend a histology approach to demonstrate diseased > mitochondria, the investigators I am working with are cardiologist dealing > with genetic defects. > > Thank you and happy holidays, > > Patsy > > > > > > > > Patsy Ruegg, HT(ASCP)QIHC > > IHCtech, LLC > > Fitzsimmons BioScience Park > > 12635 Montview Blvd. Suite 215 > > Aurora, CO 80010 > > P-720-859-4060 > > F-720-859-4110 > > email pruegg@ihctech.net > > website www.ihctech.net > > IHC Resource Group www.ihcrg.org > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From kalschev <@t> svm.vetmed.wisc.edu Wed Jan 2 13:03:01 2008 From: kalschev <@t> svm.vetmed.wisc.edu (Vicki Kalscheur) Date: Wed Jan 2 13:04:27 2008 Subject: [Histonet] bone holders Message-ID: <00b901c84d72$185b8280$c5d76880@vetmed.wisc.edu> The surgical instrument, KERN bone holding forceps with folding ratchet, come in length 6" and length 9.5" works well for band saw cutting. Roboz Surgical Instruments Co., Inc. is where we purchase for the veterinary hospital, however, there are many excellent suppliers. V. Kalscheur From abilger <@t> ptd.net Wed Jan 2 13:11:01 2008 From: abilger <@t> ptd.net (Andrea C. Bilger) Date: Wed Jan 2 13:09:46 2008 Subject: [Histonet] microwave processing Message-ID: <012901c84d73$43504150$0300a8c0@andrea> Histonetters, My lab will be setting up a microwave processor (Milestone Histo 5) shortly and we have some questions for those of you already microwave processing. Will microwave processing nullify the FDA approval of the Her2 neu test? We still plan to fix tissue completely in formalin before processing. Are there any other regulations or pitfalls we need to be aware of. Thanks for your assistance. Andrea From NMargaryan <@t> childrensmemorial.org Wed Jan 2 13:46:34 2008 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Wed Jan 2 13:43:43 2008 Subject: [Histonet] Barr body in Cell/culture - chromatin differentiation Message-ID: Happy New Year to everyone!!! I need help with some technique. Do you know a method how do recognize Barr body in Cell/culture which is mean we need a method to stain chromatin to differentiate male from female? Naira V. Margaryan, D.V.M., Ph.D. Research Scientist Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3394 Tel: 773-755-6340 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 From laurie.colbert <@t> huntingtonhospital.com Wed Jan 2 14:02:06 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed Jan 2 14:02:30 2008 Subject: [Histonet] ASCP HT Certification Message-ID: <57BE698966D5C54EAE8612E8941D76830235064A@EXCHANGE3.huntingtonhospital.com> I have two co-workers that are sending in their CMP Declaration Forms to the ASCP to maintain their HT registry. They have received conflicting information as to whether or not they need to send in a list of the classes, teleconferences, etc. that they have taken for the necessary points. What have others done - just sent in the first page of the CMP Declaration Form or also sent in a list of classes? Thanks, Laurie Colbert From safety.trainorlab <@t> mts.net Wed Jan 2 14:54:28 2008 From: safety.trainorlab <@t> mts.net (SAFETY TRAINORLAB) Date: Wed Jan 2 14:56:38 2008 Subject: [Histonet] Re: Cytology Message-ID: <000a01c84d81$aa47a0c0$19c809c0@tlabs> I am a Cytotechnologist working in Manitoba, Canada. Our provincial regulations are very clear on improperly labeled pap slides. The slide must be labeled with the patient personal health number. If the number does not match or there is no number on the slide, the slide must be destroyed by the lab and the clinician be notified that the slide was mislabeled and destroyed. Adam Chrobak BSc, MLT (Cytology) From rjbuesa <@t> yahoo.com Wed Jan 2 15:00:58 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 2 15:01:21 2008 Subject: [Histonet] BAL specimens In-Reply-To: <979FF5962E234F45B06CF0DB7C1AABB2144AC73E@chi2k3ms01.columbuschildrens.net> Message-ID: <386741.94534.qm@web61217.mail.yahoo.com> Perhaps it is true that they have not changed procedure, but they should look at the cleanliness of the slides, maybe some are dirty/oilly Ren? J. "Houston, Ronald" wrote: --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From rjbuesa <@t> yahoo.com Wed Jan 2 15:25:52 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 2 15:26:15 2008 Subject: [Histonet] ISO certification. Message-ID: <311654.65482.qm@web61219.mail.yahoo.com> Happy New Year Histonetters! Question: is any of the laboratories you work in certified for compliance with ISO9001:2000 standard? Ren? J. --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From akemiat3377 <@t> yahoo.com Wed Jan 2 15:35:29 2008 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Wed Jan 2 15:35:51 2008 Subject: [Histonet] ISO certification. In-Reply-To: <311654.65482.qm@web61219.mail.yahoo.com> Message-ID: <665945.23419.qm@web31307.mail.mud.yahoo.com> Hi Rene', Biocare Medical histology and R&D Lab has been ISO certified for several year. I was one of their ISO auditors. Regards, Akemi --- Rene J Buesa wrote: > Happy New Year Histonetters! > > Question: is any of the laboratories you work in > certified for compliance with ISO9001:2000 standard? > > Ren? J. > > > > > --------------------------------- > Looking for last minute shopping deals? Find them > fast with Yahoo! Search. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Akemi Allison-Tacha, BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing Tele: (925)788-0900 E-Mail: akemiat3377@yahoo.com From ACohen <@t> holyredeemer.com Wed Jan 2 14:51:21 2008 From: ACohen <@t> holyredeemer.com (Alisa Cohen) Date: Wed Jan 2 15:36:25 2008 Subject: [Histonet] Career Opportunity Message-ID: <8cdb80c9000918dc@HolyRedeemer.com> The Histology department of Holy Redeemer Hospital and Medical Center, located in Huntingdon Valley, PA has an immediate job opening for a full time Histotechnician. The laboratory has windows with beautiful views of the surrounding valley. The lab is extremely well equipped, well ventilated, very automated, and uses CoPath pathology software. We are seeking an HT or HT eligible technician for straight day shifts; no weekends, no holidays, and no on call. Some small grossing experience or IHC experience is a plus. We offer competitive salary and comprehensive benefits to qualified candidates. For immediate consideration, apply online at www.holyredeemer.com . EOE. --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From katherine_dubrall <@t> yahoo.com Wed Jan 2 15:45:47 2008 From: katherine_dubrall <@t> yahoo.com (kay dubrall) Date: Wed Jan 2 15:46:10 2008 Subject: [Histonet] ASCP HT Certification In-Reply-To: <57BE698966D5C54EAE8612E8941D76830235064A@EXCHANGE3.huntingtonhospital.com> Message-ID: <500808.3073.qm@web54203.mail.re2.yahoo.com> I sent my CM paperwork in a couple of months ago, and I did list the classes and coursework I'd done over the past few years. I'm pretty sure they asked for it on the forms, otherwise I don't know that I would have thought to list everything the way I did. Besides, it can't hurt. It forced me to gather everything in one place, which will no doubt make things easier if I'm ever subject to an audit. what's kay is kay - j3 --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From sbreeden <@t> nmda.nmsu.edu Wed Jan 2 15:46:53 2008 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Jan 2 15:47:19 2008 Subject: [Histonet] Autostaining issues Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E6101@nmdamailsvr.nmda.ad.nmsu.edu> Let me be as succinct as possible without installing a prejudice to the answer. I have an automatic stainer. I had a DI system installed with the explicit purpose of preventing calcium build-up in the stainer and causing me pain and agony in keeping it de-crusted. I took the manual stain line solutions/times and translated it to the stainer; I have tweaked and fiddled but the stains look as if they were done on necrotic or autolytic tissues! So I pondered reasons and fiddled/tweaked more. I hit on pH- I checked the DI water (pH 5.12!!), and the tap water (pH 7.98). I thought that was the solution to my problem, so I cut back on time in acid alcohol and cut back in wash times. No luck. Changed hematoxylin vendors. Nope. Made up 0.5% acid alcohol. Nada. Increased time in ammonia water. Not that either. The stainer vendor has been very accessible but we have not yet solved the poor staining - and it's NOT the stainer's fault. I've been on the phone with the DI cartridge supplier and he's telling me that pH may not be a factor because the DI filter adds elements (sodium?) but removes elements (calcium) and that the water contaminates the reading anyway. So I'm turning to my Panel of Experts. Is it pH, as I suspect? Is it something else? I'm facing a large workload for a project this spring and would like to use the autostainer - as well as be able to use it for the daily surgicals and necropsies. I know there's someone out there that can help me figure this out! Thanks! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From gmartin <@t> marshallmedical.org Wed Jan 2 16:10:17 2008 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Wed Jan 2 16:10:38 2008 Subject: [Histonet] oven Message-ID: <6ED9D4252F278841A0593D3D788AF24C0175A448@mailsvr.MARSHMED.local> We have decided to purchase a gravity convection oven. We are wondering if you have to vent the oven if it is used to help with special staining processes (GMS). Also any recommendation on a low cost oven and where we might get one. As always Thanks, and let's have a good New Year! Gary California From lpwenk <@t> sbcglobal.net Wed Jan 2 17:17:57 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed Jan 2 17:18:21 2008 Subject: [Histonet] ASCP HT Certification In-Reply-To: <57BE698966D5C54EAE8612E8941D76830235064A@EXCHANGE3.huntingtonhospital.com> Message-ID: <001f01c84d95$b5ffb880$0202a8c0@HPPav2> The CMP form is 4 pages, which includes the signature page. http://ascp.org/Certification/CMP/pdf/cmp_declaration.pdf All 4 pages must be sent in. Under the various categories, they have to write in course title and dates. If a category doesn't apply (such as, they didn't take a university course), then they can write in N/A. This information can be found in the CMP booklet. http://ascp.org/Certification/CMP/pdf/cmp_booklet.pdf Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Wednesday, January 02, 2008 3:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ASCP HT Certification I have two co-workers that are sending in their CMP Declaration Forms to the ASCP to maintain their HT registry. They have received conflicting information as to whether or not they need to send in a list of the classes, teleconferences, etc. that they have taken for the necessary points. What have others done - just sent in the first page of the CMP Declaration Form or also sent in a list of classes? Thanks, Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From max_histo_00 <@t> yahoo.it Wed Jan 2 18:02:30 2008 From: max_histo_00 <@t> yahoo.it (Massimo) Date: Wed Jan 2 18:02:56 2008 Subject: [Histonet] Disposable microtome blades Message-ID: <667804.43356.qm@web23301.mail.ird.yahoo.com> Hi all, I am an amateur naturalist. I have made by myself a microtome and I also use to honing the blade with results quite good. Now I am testing a disposable blade. It was a kindly gift of a friend of mine who works in an histology laboratory. I wonder where could I find disposable blades because it seems they are working well . Otherwise it is quite difficult to make and honing a blade by myself. Does anybody know a factory that produces such kind of blades? Any suggestions would be greatly appreciated. Thank You. Massimo --------------------------------- --------------------------------- L'email della prossima generazione? Puoi averla con la nuova Yahoo! Mail From rjbuesa <@t> yahoo.com Thu Jan 3 07:27:23 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 3 07:27:50 2008 Subject: [Histonet] oven In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C0175A448@mailsvr.MARSHMED.local> Message-ID: <510671.45580.qm@web61221.mail.yahoo.com> If you are buying the oven to cut in staining time for HC procedures, I think you should buy a microwave oven instead. You will cut much more time with the MW oven and some models are vented and that is something you have to have if doing HC in a heated environment. Ren? J. "Martin, Gary" wrote: --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From rjbuesa <@t> yahoo.com Thu Jan 3 07:35:19 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 3 07:35:42 2008 Subject: [Histonet] Disposable microtome blades In-Reply-To: <667804.43356.qm@web23301.mail.ird.yahoo.com> Message-ID: <614349.44052.qm@web61221.mail.yahoo.com> It is always "refreshing" to read something like this: somebody manufacturing a microtome and using it with good results. Contact the Italian representative of Leica Microsystems, they distribute disposable blades. You can find them in the web. Ren? J. Massimo wrote: --------------------------------- --------------------------------- L'email della prossima generazione? Puoi averla con la nuova Yahoo! Mail _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From rjbuesa <@t> yahoo.com Thu Jan 3 07:51:07 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 3 07:51:30 2008 Subject: [Histonet] Autostaining issues In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E6101@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <759513.15896.qm@web61218.mail.yahoo.com> I have to assume that your autostainer has continuous water flow and if it does not (is of the static type) your problem most likely can be solved by installing a continuous flow system or changing the water after each run. Which one type is yours? Ren? J. "Breeden, Sara" wrote: --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From HornHV <@t> archildrens.org Thu Jan 3 08:13:52 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Jan 3 08:15:16 2008 Subject: [Histonet] BAL specimens In-Reply-To: <979FF5962E234F45B06CF0DB7C1AABB2144AC73E@chi2k3ms01.columbuschildrens.net> References: <979FF5962E234F45B06CF0DB7C1AABB2144AC73E@chi2k3ms01.columbuschildrens.net> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82A6B@EMAIL.archildrens.org> BAL specimens can be difficult to prepare. Some are very thick and that is probably the cause of the seeming poor preparation. We process our own cytospins so we have more control over the slide prep. Sometimes the preps need diluting, sometimes fewer drops need to be used or even more drops when the specimen is not very cellular. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Wednesday, January 02, 2008 11:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BAL specimens Is anyone having difficulty with cells staying on the slide during GMS staining? There is no problem with AFB, Gram, Oil Red O, Giemsa. This is only something that has become troublesome within the last couple of months. Our hematology lab insists they have not changed any part of the cytospin preparation, although some of the preps look suspicious for poor preparation with uneven cellular/exudate distribution on the slide. Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 Ronald.Houston@NationwideChildrens.org Columbus Children's Hospital is now Nationwide Children's Hospital www.NationwideChildrens.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmiller <@t> physlab.com Thu Jan 3 08:51:33 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Thu Jan 3 08:51:58 2008 Subject: [Histonet] waterbath/ workstation Message-ID: <000001c84e18$22335430$3d02a8c0@plab.local> Hi Histonetters! I wondered who has log sheets documenting cutting station maintenance. Like cleaning and disinfecting the waterbath etc?? We have one but, is it really necessary for CAP regs?? Thanks, Cheri PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From rjbuesa <@t> yahoo.com Thu Jan 3 08:57:55 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 3 08:58:19 2008 Subject: [Histonet] waterbath/ workstation In-Reply-To: <000001c84e18$22335430$3d02a8c0@plab.local> Message-ID: <952742.67232.qm@web61211.mail.yahoo.com> Some documentation about temperature is required. You could have a "general" log stating that all water baths received special cleaning (even sometimes painting) / disinfecting at the end of the week (as I used to have). Ren? J. Cheri Miller wrote: Hi Histonetters! I wondered who has log sheets documenting cutting station maintenance. Like cleaning and disinfecting the waterbath etc?? We have one but, is it really necessary for CAP regs?? Thanks, Cheri PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From LSebree <@t> uwhealth.org Thu Jan 3 09:08:21 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Thu Jan 3 09:08:50 2008 Subject: [Histonet] waterbath/ workstation In-Reply-To: <000001c84e18$22335430$3d02a8c0@plab.local> Message-ID: We do not have such logs. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Thursday, January 03, 2008 8:52 AM To: 'Histonet' Subject: [Histonet] waterbath/ workstation Hi Histonetters! I wondered who has log sheets documenting cutting station maintenance. Like cleaning and disinfecting the waterbath etc?? We have one but, is it really necessary for CAP regs?? Thanks, Cheri PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Thu Jan 3 09:22:22 2008 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Jan 3 09:21:17 2008 Subject: [Histonet] ISO certification. In-Reply-To: <311654.65482.qm@web61219.mail.yahoo.com> Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F50C@lmhsmail.lmhealth.org> Yes, we and have been for about 2 years now. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Wednesday, January 02, 2008 4:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ISO certification. Happy New Year Histonetters! Question: is any of the laboratories you work in certified for compliance with ISO9001:2000 standard? Ren? J. --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Linke_Noelle <@t> Allergan.com Thu Jan 3 09:25:55 2008 From: Linke_Noelle <@t> Allergan.com (Linke_Noelle) Date: Thu Jan 3 09:27:25 2008 Subject: [Histonet] CD31 in the rat Message-ID: <5C3DA4BE34AA0641BAA10A7C1478B60527D021@IRMAIL132.irvine.allergan.com> Hi everyone! Has anyone found an acceptable CD31 antibody for FFPE rat tissue since the tragic death of santa cruz's goat and their last hope of producing an antibody that actually works?? Noelle No?lle Linke, MS, HTL(ASCP)QIHC Allergan, Inc 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5568 From talulahgosh <@t> gmail.com Thu Jan 3 09:37:09 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Jan 3 09:37:33 2008 Subject: [Histonet] CD31 in the rat In-Reply-To: <5C3DA4BE34AA0641BAA10A7C1478B60527D021@IRMAIL132.irvine.allergan.com> References: <5C3DA4BE34AA0641BAA10A7C1478B60527D021@IRMAIL132.irvine.allergan.com> Message-ID: This made me laugh, one goat dies and your antibody is lost. On Jan 3, 2008 10:25 AM, Linke_Noelle wrote: > Hi everyone! > > Has anyone found an acceptable CD31 antibody for FFPE rat tissue since the tragic death of santa cruz's goat and their last hope of producing an antibody that actually works?? > > Noelle > -- "Prosperity ripened the principle of decay...and, as soon as time or accident and removed the artificial supports, the stupendous fabric yielded to the pressure of its own weight...instead of inquiring why the Roman Empire was destroyed we should rather be surprised that it has subsisted for so long" -Edward Gibbon, Decline and Fall of the Roman Empire From bliven.laura <@t> marshfieldclinic.org Thu Jan 3 09:41:24 2008 From: bliven.laura <@t> marshfieldclinic.org (Bliven, Laura) Date: Thu Jan 3 09:41:56 2008 Subject: [Histonet] k-9 Vet Plasma Marker Message-ID: <200801031541.m03FfZZh002782@spamfilt.mfldclin.edu> What's the best plasma cell marker for tumors on dog tissue? Additional info would be appreciated as to antigen retrieval and dilution, even though we'll be testing out various steps. Thanks, Laura Laura Bliven Histology Lab/IHC Marshfield Laboratories 1000 N. Oak Ave. Marshfield, WI 54449 From Carmen.Wynn <@t> us.astellas.com Thu Jan 3 10:26:06 2008 From: Carmen.Wynn <@t> us.astellas.com (Wynn, Carmen) Date: Thu Jan 3 10:27:35 2008 Subject: [Histonet] CD68 In-Reply-To: <20071220230837.086C0FF007D@mail157-sin.bigfish.com> Message-ID: Hello, I have had good results with AbD Serotec's CD68 (cat# MCA1957) on frozen acetone fixed mouse heart allografts with 2-step indirect immunohistochemical staining. You should probably titrate to a suitable dilution. I have not tried this antibody on paraffin sections. Good Luck, Carmen Wynn, M.S., Senior Scientist Astellas Research Institute of America, LLC. (ARIA) Illinois Science and Technology Park 8045 Lamon Ave Skokie, IL 60077 Direct: 847-933-7419 Main: 847-933-7400 Fax: 847-933-7401 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, December 20, 2007 5:09 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 49, Issue 22 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. IHC Technologist Position at PhenoPath (Seattle, WA) (Owen, Michael P) 2. Re: Osmometer (Robert Chiovetti) 3. Re: CD68 (Majid ghoddusi) 4. Happy Holidays! (arnie.jimenez@vel-lab.com) 5. RE: CD68 (Josh Wray) 6. MICROWAVES (Janice Mitchell) 7. Off Topic: NO SANTA CLAUS (Ingles Claire) 8. RE: Off Topic: NO SANTA CLAUS (Thomas Jasper) 9. RE: Off Topic: NO SANTA CLAUS (Nancy Lemke) 10. Tissue Tek anti-roll device (Shirley Powell) 11. Re: MICROWAVES (Phil McArdle) 12. Jacquelyn Grewe/Staff/OhioHealth is out of the office . (JGREWE@OhioHealth.com) 13. Re: Tissue Tek anti-roll device (Jennifer MacDonald) 14. Christmas Greetings, and thanks to... (mtitford@aol.com) 15. IHC for VIAS (Godfrey Guerzon) 16. RE: SPAM-LOW: [Histonet] IHC for VIAS (Douglas D Deltour) 17. MITF (Douglas D Deltour) 18. Kathy Abels/ops/diag/sial is out of the office. (Kathy Abels) 19. Re: IHC for VIAS (Patti Loykasek) 20. IgA-FITC (Houston, Ronald) 21. Off topic: Free samples of chemiluminescent substrates (richardkuzma@michdiag.com) 22. Re: IHC for VIAS (Godfrey Guerzon) 23. RE: IHC for VIAS (Annette Hall) ---------------------------------------------------------------------- Message: 1 Date: Thu, 20 Dec 2007 13:04:17 -0500 From: "Owen, Michael P" Subject: [Histonet] IHC Technologist Position at PhenoPath (Seattle, WA) To: "Histonet" Message-ID: <449E51C6DA0AD840B44F57C7A6EB07BF0422035E@FMD3VS022.fda.gov> Content-Type: text/plain; charset=us-ascii Immunohistochemistry (IHC) Technologist craigslist Seattle-Tacoma / jobs / biotech and science http://seattle.craigslist.org http://seattle.craigslist.org/see/sci/514227920.html ------------------------------------------------------------------------ -------- Reply to: jobs@phenopath.com Date: 2007-12-19, 9:46AM PST PhenoPath Laboratories, a national pathology reference laboratory, has an opportunity in the clinical immunohistochemistry division for a full-time IHC technologist, day shift. We are in a state-of-the-art facility located in Seattle, WA. JOB DESCRIPTION: Responsibilities may include performing immunohistochemistry, and immunofluorescence on patient samples and tissue sectioning (paraffin and frozen). Participation in the development of new tests or technologies, as well as participation in clinical research projects is also included. Quality control, safety, and preventive maintenance procedures and documentation are required elements of this position as well. REQUIRED SKILLS/EXPERIENCE: Strong preference given to ASCP certified (or certification-eligible) laboratory techs. Trained laboratory techs of any discipline are encouraged to apply (histotech, med tech, cytotech). PhenoPath Laboratories is committed to hiring the best person for the job. PhenoPath Laboratories offers a competitive compensation and benefits package, including 401k. TO APPLY, PLEASE E-MAIL OR FAX COMPLETED APPLICATION FOR EMPLOYMENT AND RESUME TO: Paul Moore, Assistant to the Director of Human Resources PhenoPath Laboratories 551 N. 34th St., Suite 100 Seattle, WA 98103 Fax: 206 374-9009 E-mail: jobs@phenopath.com (Preferred method of application) No phone calls about this job, please. Location: Seattle, WA Compensation: DOE Principals only. Recruiters, please don't contact this job poster. Please, no phone calls about this job! Please do not contact job poster about other services, products or commercial interests. PostingID: 514227920 Michael P. Owen, Regulatory Microbiologist U.S. FDA Pacific Regional Lab Northwest 22201 23rd Drive SE Bothell, WA 98021-4421 Phone: 425-483-4865 E-Mail: michael.owen@fda.hhs.gov ------------------------------ Message: 2 Date: Thu, 20 Dec 2007 10:11:16 -0800 (PST) From: Robert Chiovetti Subject: Re: [Histonet] Osmometer To: Mauricio Avigdor , histonet@lists.utsouthwestern.edu Message-ID: <756859.64500.qm@web58911.mail.re1.yahoo.com> Content-Type: text/plain; charset=us-ascii Hi Mauricio, Well, that's interesting. The "more modern techniques" still require an instrument to measure osmolality! Maybe you need to ask someone else in the laboratory... There are two basic ways to measure osmolality: vapor pressure and freezing point depression. In the vapor pressure instruments, you soak a small filter paper circle with the solution, put it in a sealed chamber and measure the amount of heat that is required to vaporize the solution and form an amount of pressure in the chamber. The freezing point depression machines usually require a little more solution. It's put in a small chamber, the chamber is chilled, and the temperature is measured at which the solution freezes. There is sometimes a small vibrating probe in the solution to agitate it while it's cooling down. At least these are the two old standby methods. There are probably more freezing point depression osmometers in use than vapor pressure osmometers, but they have both been around for many years! Maybe you should ask someone else in another lab? Cheers, Bob Robert (Bob) Chiovetti, Ph.D. Southwest Precision Instruments See What's New on Our Website! Arizona's Microscopy Resource 132 North Elster Drive Tucson, AZ 85710-3212 Tel./Fax 520-546-4986 Member, Arizona Small Business Association (www.asba.com) ----- Original Message ---- I asked to borrow an osmometer from another lab, and was told that they no longer use these, as they have moved on to "more modern techniques". I am really curious to know what these techniques could be. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ ____________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ ------------------------------ Message: 3 Date: Thu, 20 Dec 2007 10:15:49 -0800 From: "Majid ghoddusi" Subject: Re: [Histonet] CD68 To: "Rene J Buesa" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 While on CD 68 subject, I have been told that this does not work on mouse tissue. In other words it is not a good IHC marker for mouse macrophages. I would appreciate advice from people who have successfuly tried this on mouse tissue. Thanks, Majid *Majid Ghoddusi, DVM, PhD* *Veterinary Pathologist* *Comparative Biosciences, Inc.* *786 Lucerne Drive, * *Sunnyvale, CA 94085* *http://www.compbio.com/* *Ph: 408-738-9265* *Fax: 408-738-9278* On Dec 20, 2007 9:58 AM, Rene J Buesa wrote: > IF you have both control and case running simultaneously and there is no > problem with the instrument (like malfunctioning over one slide and not over > the other, which is very unlikely) your problem is with your control. > I always used tonsils coming from surgey and ususally older than 2 yo > patients. > I used Dako IgMo Ab at 1:1000 with pH6 HIER > Try a new control tissue (fresh sections if at all possible). > Ren J. > > Cheri Miller wrote: > > > > > > > --------------------------------- > Looking for last minute shopping deals? Find them fast with Yahoo! > Search. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 4 Date: 20 Dec 2007 12:11:50 -0600 From: arnie.jimenez@vel-lab.com Subject: [Histonet] Happy Holidays! To: histonet@lists.utsouthwestern.edu Message-ID: <20071220181150.19040.qmail@ux-vhost05.dllstx2.theplanet.com> Content-Type: text/plain; charset="UTF-8" This is an automated response. Thank you for contacting Vel-Lab Research. We are shutdown for the holidays. We use this time to give our employees a well deserved break, we also take the opportunity to thouroghly clean our facilities and calibrate all our equipment. Your project is our top priority and we will return to it as soon as possible. I will personally return to the lab Dec. 27th and will attend to any urgent issues then. The full lab will be back the first week of January. Thank you for your understanding. Happy Holidays from everyone at Vel-Lab Arnie Jimenez Owner Vel-Lab Research ------------------------------ Message: 5 Date: Thu, 20 Dec 2007 13:27:35 -0500 From: "Josh Wray" Subject: [Histonet] RE: CD68 To: histonet@lists.utsouthwestern.edu Message-ID: <883927cf0712201027u1dc19f6o998092564f7eebec@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 We too had a problem with tonsil for a CD68 control. We decided to run a lymph node as the QC. It worked out much better. Josh Wray HT(ASCP) Ameripath Indianapolis ------------------------------ Message: 6 Date: Thu, 20 Dec 2007 13:43:19 -0500 From: "Janice Mitchell" Subject: [Histonet] MICROWAVES To: Message-ID: Content-Type: text/plain; charset=US-ASCII Cap requests "microwave devices be periodically monitored for reproducibility" what is considered periodically? Thanks, Janice ------------------------------ Message: 7 Date: Thu, 20 Dec 2007 12:59:10 -0600 From: "Ingles Claire" Subject: [Histonet] Off Topic: NO SANTA CLAUS To: Message-ID: <08A0A863637F1349BBFD83A96B27A50A1200B7@uwhis-xchng3.uwhis.hosp.wisc.edu > Content-Type: text/plain; charset="iso-8859-1" Sorry if you are not Christian. You can delete, but I think Santa just has lots of different names around the world. Everyone - remember what you have that never cost anything. Merry Christmas greetings and warm wishes to all. Claire Ingles lifelong Santa's helper I remember my first Christmas adventure with Grandma. I was just a kid. I remember tearing across town on my bike to visit her on the day my big sister dropped the bomb: "There is no Santa Claus," she jeered. "Even dummies know that!" My Grandma was not the gushy kind, never had been. I fled to her that day because I knew she would be straight with me. I knew Grandma always told the truth, and I knew that the truth always went down a whole lot easier when swallowed with one of her worl d-famous cinnamon buns. I knew they were world-famous, because Grandma said so. It had to be true. Grandma was home, and the buns were still warm. Between bites, I told her everything. She was ready for me. "No Santa Claus! !" she snorted. "Ridiculous! " Don't believe it. That rumor has been going around for years, and it makes me mad, plain mad.' Now, put on your coat, and let's go." "Go? Go where, Grandma?" I asked. I hadn't even finished my second world-famous, cinnamon bun. "Where" turned out to be Kerby's General Store, the one store in town that had a little bit of just about everything. As we walked through its doors, Grandma handed me ten dollars. That was a bundle in those days. "Take this money," she said, "and buy something for someone who needs it. I'll wait for you in the car." Then she turned and walked out of Kerby's. I was only eight years old. I'd often gone shopping with my mother, but never had I shopped for anything all by myself. The store seemed big and crowded, full of people scrambling to finish their Christmas shopping. For a few moments I just stood there, confused, clutching that ten-dollar bill, wondering what to buy, and who on earth to buy it for. I thought of everybody I knew: my family, my friends, my neighbors, the kids at school, the people who went to my church. I was just about thought out, when I suddenly thought of Bobby Decker. He was a kid with bad breath and messy hair, and he sat right behind me in Mrs. Pollock's grade-two class. Bobby Decker didn't have a coat. I knew that because he never went out for recess during the winter. His mother always wrote a note, telling the teacher that he had a cough, but all we kids knew that Bobby Decker didn't have a cough, and he didn't have a coat. I fingered the ten-dollar bill with growing excitement. I would buy Bobby Decker a coat! I settled on a red corduroy one that had a hood to it. It looked real warm, and he would like that. "Is this a Christmas present for someone?" the lady behind the counter asked kindly, as I laid my ten dollars down. "Yes," I replied shyly. "It's ... for Bobby." The nice lady smiled at me. I didn't get any change, but she put the coat in a bag and wished me a Merry Christmas. That evening, Grandma helped me wrap the coat in Christmas paper and ribbons (a little tag fell out of the coat, and Grandma tucked it in her Bible) and wrote, "To Bobby, From Santa Claus", on a tag-- Grandma said that Santa always insisted on secrecy. Then she drove me over to Bobby Decker's house, explaining as we went that I was now and forever officially one of Santa's helpers. Grandma parked down the street from Bobby's house, and she and I crept noiselessly and hid in the bushes by his front walk. Then Gr andma gave me a nudge. "All right, Santa Claus," she whispered, "get going." I took a deep breath, dashed for his front door, and threw the present down on his step. I pounded his door and flew back to the safety of the bushes and Grandma. Together we waited breathlessly in the darkness for the front door to open. Finally it did, and there stood Bobby. Fifty years haven't dimmed the thrill of those moments spent shivering beside my Grandma, in Bobby Decker's bushes. That night, I realized that those awful rumors about Santa Claus were just what Grandma said they were: "Ridiculous". Santa was alive and well, and we were on his team. I still have the Bible, with the tag tucked inside: $19.95. -------------------------------------------------------------------- He who has no Christmas in his heart will never find Christmas under a tree. Have a wonderful holiday season. Merry Christmas and a Happy New Year!! ------------------------------ Message: 8 Date: Thu, 20 Dec 2007 12:07:49 -0800 From: "Thomas Jasper" Subject: RE: [Histonet] Off Topic: NO SANTA CLAUS To: "Ingles Claire" Cc: histonet@lists.utsouthwestern.edu Message-ID: <90354A475B420441B2A0396E5008D4965E201A@copc-sbs.COPC.local> Content-Type: text/plain; charset="us-ascii" Thanks Claire, totally awesome. Merry Christmas and Happy New Year from one of Santa helpers to another. Tom Jasper Bend, OR (by way of northern WI) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Thursday, December 20, 2007 10:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Off Topic: NO SANTA CLAUS Sorry if you are not Christian. You can delete, but I think Santa just has lots of different names around the world. Everyone - remember what you have that never cost anything. Merry Christmas greetings and warm wishes to all. Claire Ingles lifelong Santa's helper I remember my first Christmas adventure with Grandma. I was just a kid. I remember tearing across town on my bike to visit her on the day my big sister dropped the bomb: "There is no Santa Claus," she jeered. "Even dummies know that!" My Grandma was not the gushy kind, never had been. I fled to her that day because I knew she would be straight with me. I knew Grandma always told the truth, and I knew that the truth always went down a whole lot easier when swallowed with one of her worl d-famous cinnamon buns. I knew they were world-famous, because Grandma said so. It had to be true. Grandma was home, and the buns were still warm. Between bites, I told her everything. She was ready for me. "No Santa Claus! !" she snorted. "Ridiculous! " Don't believe it. That rumor has been going around for years, and it makes me mad, plain mad.' Now, put on your coat, and let's go." "Go? Go where, Grandma?" I asked. I hadn't even finished my second world-famous, cinnamon bun. "Where" turned out to be Kerby's General Store, the one store in town that had a little bit of just about everything. As we walked through its doors, Grandma handed me ten dollars. That was a bundle in those days. "Take this money," she said, "and buy something for someone who needs it. I'll wait for you in the car." Then she turned and walked out of Kerby's. I was only eight years old. I'd often gone shopping with my mother, but never had I shopped for anything all by myself. The store seemed big and crowded, full of people scrambling to finish their Christmas shopping. For a few moments I just stood there, confused, clutching that ten-dollar bill, wondering what to buy, and who on earth to buy it for. I thought of everybody I knew: my family, my friends, my neighbors, the kids at school, the people who went to my church. I was just about thought out, when I suddenly thought of Bobby Decker. He was a kid with bad breath and messy hair, and he sat right behind me in Mrs. Pollock's grade-two class. Bobby Decker didn't have a coat. I knew that because he never went out for recess during the winter. His mother always wrote a note, telling the teacher that he had a cough, but all we kids knew that Bobby Decker didn't have a cough, and he didn't have a coat. I fingered the ten-dollar bill with growing excitement. I would buy Bobby Decker a coat! I settled on a red corduroy one that had a hood to it. It looked real warm, and he would like that. "Is this a Christmas present for someone?" the lady behind the counter asked kindly, as I laid my ten dollars down. "Yes," I replied shyly. "It's ... for Bobby." The nice lady smiled at me. I didn't get any change, but she put the coat in a bag and wished me a Merry Christmas. That evening, Grandma helped me wrap the coat in Christmas paper and ribbons (a little tag fell out of the coat, and Grandma tucked it in her Bible) and wrote, "To Bobby, From Santa Claus", on a tag-- Grandma said that Santa always insisted on secrecy. Then she drove me over to Bobby Decker's house, explaining as we went that I was now and forever officially one of Santa's helpers. Grandma parked down the street from Bobby's house, and she and I crept noiselessly and hid in the bushes by his front walk. Then Gr andma gave me a nudge. "All right, Santa Claus," she whispered, "get going." I took a deep breath, dashed for his front door, and threw the present down on his step. I pounded his door and flew back to the safety of the bushes and Grandma. Together we waited breathlessly in the darkness for the front door to open. Finally it did, and there stood Bobby. Fifty years haven't dimmed the thrill of those moments spent shivering beside my Grandma, in Bobby Decker's bushes. That night, I realized that those awful rumors about Santa Claus were just what Grandma said they were: "Ridiculous". Santa was alive and well, and we were on his team. I still have the Bible, with the tag tucked inside: $19.95. -------------------------------------------------------------------- He who has no Christmas in his heart will never find Christmas under a tree. Have a wonderful holiday season. Merry Christmas and a Happy New Year!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Thu, 20 Dec 2007 15:22:11 -0500 From: "Nancy Lemke" Subject: RE: [Histonet] Off Topic: NO SANTA CLAUS To: "Thomas Jasper" , "Ingles Claire" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Merry Christmas to you both and to all Santa's helpers, big and small! Nancy Lemke Research Coordinator Hermelin Brain Tumor Center Henry Ford Hospital Detroit -----Original message----- From: "Thomas Jasper" tjasper@copc.net Date: Thu, 20 Dec 2007 16:09:31 -0500 To: "Ingles Claire" CIngles@uwhealth.org Subject: RE: [Histonet] Off Topic: NO SANTA CLAUS > Thanks Claire, totally awesome. Merry Christmas and Happy New Year from > one of Santa helpers to another. > Tom Jasper > Bend, OR (by way of northern WI) > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles > Claire > Sent: Thursday, December 20, 2007 10:59 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Off Topic: NO SANTA CLAUS > > > Sorry if you are not Christian. You can delete, but I think Santa just > has lots of different names around the world. Everyone - remember what > you have that never cost anything. Merry Christmas greetings and warm > wishes to all. > > Claire Ingles > lifelong Santa's helper > > > > I remember my first Christmas > adventure with Grandma. > I was just a kid. > > I remember tearing across town > on my bike to visit her on the day my big sister dropped the bomb: > > "There is no Santa Claus," she > jeered. "Even dummies know that!" > > My Grandma was not the gushy > kind, never had been. > > I fled to her that day because I > knew she would be straight with me. I knew Grandma always told the > truth, and I knew that the truth always went down a whole lot easier > when swallowed with one of her worl d-famous cinnamon buns. > > I knew they were world-famous, > because Grandma said so. > It had to be true. > Grandma was home, and the buns > were still warm. > > Between bites, I told her > everything. She was ready for me. > "No Santa Claus! !" she snorted. > "Ridiculous! > " Don't believe it. That rumor > has been going around for years, and it makes me mad, plain mad.' > > Now, put on your coat, and let's > go." > "Go? Go where, Grandma?" I > asked. > I hadn't even finished my second > world-famous, cinnamon bun. > > "Where" turned out to be Kerby's > General Store, the one store in town that had a little bit of just about > everything. As we walked through its doors, Grandma handed me ten > dollars. That was a bundle in those days. > > "Take this money," she said, > "and buy something for someone who needs it. I'll wait for you in the > car." > > Then she turned and walked out > of Kerby's. I was only eight years old. I'd often gone shopping with my > mother, but never had I shopped for anything all by myself. > > The store seemed big and > crowded, full of people scrambling to finish their Christmas shopping. > For a few moments I just stood there, confused, clutching that > ten-dollar bill, wondering what to buy, and who on earth to buy it for. > > I thought of everybody I knew: > my family, my friends, my neighbors, the kids at school, the people who > went to my church. > > I was just about thought out, > when I suddenly thought of Bobby Decker. He was a kid with bad breath > and messy hair, and he sat right behind me in Mrs. Pollock's grade-two > class. Bobby Decker didn't have a coat. > > I knew that because he never > went out for recess during the winter. His mother always wrote a note, > telling the teacher that he had a cough, but all we kids knew that Bobby > Decker didn't have a cough, and he didn't have a coat. > > I fingered the ten-dollar bill > with growing excitement. I would buy Bobby Decker a coat! I settled on> a > red corduroy one that had a hood to it. It looked real warm, and he > would like that. > > "Is this a Christmas present for > someone?" the lady behind the counter asked kindly, as I laid my ten > dollars down. > > "Yes," I replied shyly. "It's > ... for Bobby." > > The nice lady smiled at me. I > didn't get any change, but she put the coat in a bag and wished me a > Merry Christmas. > > That evening, Grandma helped me > wrap the coat in Christmas paper and ribbons (a little tag fell out of > the coat, and Grandma tucked it in her Bible) and wrote, "To Bobby, From > Santa Claus", on a tag-- Grandma said that Santa always insisted on > secrecy. > > Then she drove me over to Bobby > Decker's house, explaining as we went that I was now and forever > officially one of Santa's helpers. > > Grandma parked down the street > from Bobby's house, and she and I crept noiselessly and hid in the > bushes by his front walk. Then Gr andma gave me a nudge. "All right, > Santa Claus," she whispered, "get going." > I took a deep breath, dashed for > his front door, and threw the present down on his step. I pounded his > door and flew back to the safety of the bushes and Grandma. > > Together we waited breathlessly > in the darkness for the front door to open. Finally it did, and there > stood Bobby. Fifty years haven't dimmed the thrill of those moments > spent shivering beside my Grandma, in Bobby Decker's bushes. > > That night, I realized that > those awful rumors about Santa Claus were just what Grandma said they > were: "Ridiculous". Santa was alive and well, and we were on his team. > > I still have the Bible, with the > tag tucked inside: $19.95. > > > -------------------------------------------------------------------- > He who has no Christmas in his > heart will never find Christmas under a tree. > > Have a wonderful holiday season. > Merry Christmas and a Happy New Year!! > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ======================================================================== ====== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ======================================================================== ====== ------------------------------ Message: 10 Date: Thu, 20 Dec 2007 15:52:58 -0500 From: Shirley Powell Subject: [Histonet] Tissue Tek anti-roll device To: histonet@lists.utsouthwestern.edu Message-ID: <01MP4ABAC5UE0012GH@Macon2.Mercer.edu> Content-Type: text/plain; charset=US-ASCII Hi Guys, I am reaching for straws, but I need to locate an anti-roll device for an older model Lab Tek, Tissue Tek cryostat. Mine finally was destroyed after ohhhhh, say 25 years. Need to replace it or rebuild it. Any help would be appreciated. Thanks Shirley Powell, HT(ASCP)HTL, QIHC Technical Director Histopathology Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 ------------------------------ Message: 11 Date: Thu, 20 Dec 2007 15:57:17 -0500 From: Phil McArdle Subject: Re: [Histonet] MICROWAVES To: Janice Mitchell Cc: histonet@lists.utsouthwestern.edu Message-ID: <476AD72D.1080302@ebsciences.com> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hi: This response is from a microwave vendor, so you've been forewarned! :-) That said, it seems analogous ISO-9000 certification: each lab is allowed to make its own determination as to the meaning of "periodically;" the procedure should be documented and assigned AND (and here's the key part) adhered to and logged! I've seen some labs with very nicely thought out and documented procedures, who got written up because they didn't follow their own procedures. When CAP began their microwave leakage measurement requirement, it specified "at least" yearly, and EBS recommended quarterly. (Arbitrary? Maybe.) As for reproducibility, I feel that yearly is not enough, daily or weekly is probably excessive, but depending upon your lab's volume, monthly or quarterly should be sufficient, unless anything unusual is noted (blown fuse, etc.). Hope this helps! Phil McArdle -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com I skate to where the puck is going to be, not to where it's been. - Wayne Gretsky You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway. So please do the right thing and make this e-mail go away. Thank you. Janice Mitchell wrote: > Cap requests "microwave devices be periodically monitored for > reproducibility" what is considered periodically? > Thanks, Janice > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Thu, 20 Dec 2007 16:01:22 -0500 From: JGREWE@OhioHealth.com Subject: [Histonet] Jacquelyn Grewe/Staff/OhioHealth is out of the office . To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 12/20/2007 and will not return until 12/24/2007. I will return Tuesday November 13 at 11 PM and will respond to your message when I return. Thanks, Jackie ------------------------------ Message: 13 Date: Thu, 20 Dec 2007 13:09:33 -0800 From: Jennifer MacDonald Subject: Re: [Histonet] Tissue Tek anti-roll device To: Shirley Powell Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Try Sakura. That is where we bought ours, just a few of years ago. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu Shirley Powell Sent by: histonet-bounces@lists.utsouthwestern.edu 12/20/2007 12:52 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Tissue Tek anti-roll device Hi Guys, I am reaching for straws, but I need to locate an anti-roll device for an older model Lab Tek, Tissue Tek cryostat. Mine finally was destroyed after ohhhhh, say 25 years. Need to replace it or rebuild it. Any help would be appreciated. Thanks Shirley Powell, HT(ASCP)HTL, QIHC Technical Director Histopathology Mercer University School of Medicine 1550 College Street Macon, GA 31207 478-301-2374 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Thu, 20 Dec 2007 16:24:32 -0500 From: mtitford@aol.com Subject: [Histonet] Christmas Greetings, and thanks to... To: histonet@lists.utsouthwestern.edu Message-ID: <8CA1165D8975232-D9C-6672@WEBMAIL-MB20.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" I would like to join in wishing everyone a happy Christmas, this time from the "Heart of Dixie", ?and to thank Dr Margraf and others?for hosting the "Histonet". Thanks also to all the subscribers whose questions and answers help keep me informed.?Life is so interesting! Mike Titford USA Pathology Mobile AL USA ________________________________________________________________________ More new features than ever. Check out the new AOL Mail ! - http://webmail.aol.com ------------------------------ Message: 15 Date: Thu, 20 Dec 2007 16:27:38 -0500 From: "Godfrey Guerzon" Subject: [Histonet] IHC for VIAS To: Cc: Message-ID: Content-Type: text/plain; charset=US-ASCII We are having problem with tissue falling off the slides when we run the breast panel on the Ventana XT for examination with the VIAS instrument. The PR, ER, Ki67 and Her2 tends to fall off. We use "plus" slides and place them in 45 degree oven overnight and they still fall of. Any suggestions? Thanks. ----------------------------------------------------------------- THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE ADDRESSEE LISTED ABOVE. This record has been disclosed in accordance with Subtitle 3 of Title 4 of the Health-General Article of the Annotated Code of Maryland. Further disclosure of medical information contained herein is prohibited. If you are neither the intended recipient nor the individual responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure of patient information is strictly prohibited. If you have received this email in error, immediately notify us by telephone or return email. ----------------------------------------------------------------- ------------------------------ Message: 16 Date: Thu, 20 Dec 2007 16:46:07 -0500 From: "Douglas D Deltour" Subject: RE: SPAM-LOW: [Histonet] IHC for VIAS To: "'Godfrey Guerzon'" , Cc: histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="us-ascii" The first question that Ventana will ask is what brand of slides are you using? If you are using the "correct slides" do you use any adhesive in your water bath? Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Godfrey Guerzon Sent: Thursday, December 20, 2007 4:28 PM To: histonet@lists.utsouthwestern.edu Cc: histonet-bounces@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] IHC for VIAS We are having problem with tissue falling off the slides when we run the breast panel on the Ventana XT for examination with the VIAS instrument. The PR, ER, Ki67 and Her2 tends to fall off. We use "plus" slides and place them in 45 degree oven overnight and they still fall of. Any suggestions? Thanks. ----------------------------------------------------------------- THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE ADDRESSEE LISTED ABOVE. This record has been disclosed in accordance with Subtitle 3 of Title 4 of the Health-General Article of the Annotated Code of Maryland. Further disclosure of medical information contained herein is prohibited. If you are neither the intended recipient nor the individual responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure of patient information is strictly prohibited. If you have received this email in error, immediately notify us by telephone or return email. ----------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Thu, 20 Dec 2007 16:55:34 -0500 From: "Douglas D Deltour" Subject: [Histonet] MITF To: Message-ID: Content-Type: text/plain; charset="us-ascii" Does anyone have a good MITF protocol for the Ventana Benchmark XT? Thanks. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. ------------------------------ Message: 18 Date: Thu, 20 Dec 2007 16:02:24 -0600 From: Kathy Abels Subject: [Histonet] Kathy Abels/ops/diag/sial is out of the office. To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" I will be out of the office starting 12/19/2007 and will not return until 01/02/2008. I will respond to your message when I return. For urgent matters, Please contact Sherry Chappell or Leigh Gaskill. This message and any files transmitted with it are the property of Sigma-Aldrich Corporation, are confidential, and are intended solely for the use of the person or entity to whom this e-mail is addressed. If you are not one of the named recipient(s) or otherwise have reason to believe that you have received this message in error, please contact the sender and delete this message immediately from your computer. Any other use, retention, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. ------------------------------ Message: 19 Date: Thu, 20 Dec 2007 14:11:40 -0800 From: Patti Loykasek Subject: Re: [Histonet] IHC for VIAS To: Godfrey Guerzon , Message-ID: Content-Type: text/plain; charset="US-ASCII" I would recommend some time in a 60-65 degree oven even if you do still want to leave them in a 37-45 degree oven overnight. That's just off the top of my head - busy day here. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > We are having problem with tissue falling off the slides when we run the > breast panel on the Ventana XT for examination with the VIAS instrument. > The PR, ER, Ki67 and Her2 tends to fall off. We use "plus" slides and > place them in 45 degree oven overnight and they still fall of. > > Any suggestions? > > Thanks. > > > ----------------------------------------------------------------- > THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED > AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE > ADDRESSEE LISTED ABOVE. > This record has been disclosed in accordance with Subtitle 3 of > Title 4 of the Health-General Article of the Annotated Code of > Maryland. Further disclosure of medical information contained > herein is prohibited. > If you are neither the intended recipient nor the individual > responsible for delivering this message to the intended > recipient, you are hereby notified that any disclosure of > patient information is strictly prohibited. > If you have received this email in error, immediately notify us > by telephone or return email. > ----------------------------------------------------------------- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ - This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ------------------------------ Message: 20 Date: Thu, 20 Dec 2007 17:18:26 -0500 From: "Houston, Ronald" Subject: [Histonet] IgA-FITC To: Message-ID: <979FF5962E234F45B06CF0DB7C1AABB214284CE9@chi2k3ms01.columbuschildrens.n et> Content-Type: text/plain; charset="us-ascii" Here's a new one for me, but I'm sure someone out there has been approached about this (at least I hope so!) Has anyone attempted to demonstrate IgA in human breast milk for fluorescence photomicroscopy? Preliminary attempts light up like a Christmas tree................. Thanks and Merry Christmas Ronnie Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 Ronald.Houston@NationwideChildrens.org Columbus Children's Hospital is now Nationwide Children's Hospital www.NationwideChildrens.org ------------------------------ Message: 21 Date: Thu, 20 Dec 2007 17:22:52 -0500 From: "richardkuzma@michdiag.com" Subject: [Histonet] Off topic: Free samples of chemiluminescent substrates To: histonet@lists.utsouthwestern.edu Message-ID: <1928.1198189372@michdiag.com> Content-Type: text/plain; charset="us-ascii" Michigan Diagnostics Is offering free evaluation samples to the research co number, so we don Luminol based HRP substrate and dioxetane based AP substrate are what were We do have other substrates for sale, including chemilumi substrates for the detection of Beta-Glucosidase, Beta-Galactosidase and Be www.michdiag.c Sorry if you think this is too off topic. Please don't flame me too badly. Best Regards, Richard Kuzma Production Manager Michigan< Royal Oak, MI. 48073 USA richardkuzma@michdiag.com www.michdiag.com (248)435-4472 (248)435-4537 ------------------------------ Message: 22 Date: Thu, 20 Dec 2007 18:02:22 -0500 From: "Godfrey Guerzon" Subject: Re: [Histonet] IHC for VIAS To: , "Patti Loykasek" Message-ID: Content-Type: text/plain; charset=US-ASCII Thanks Patti, We have tried this method of 30 minutes in a 60degree oven and overnight at 37-40 degree oven - it gives us better results but still part of the tissue is falling off. Thanks. Godfrey >>> Patti Loykasek 12/20/2007 5:11 PM >>> I would recommend some time in a 60-65 degree oven even if you do still want to leave them in a 37-45 degree oven overnight. That's just off the top of my head - busy day here. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > We are having problem with tissue falling off the slides when we run the > breast panel on the Ventana XT for examination with the VIAS instrument. > The PR, ER, Ki67 and Her2 tends to fall off. We use "plus" slides and > place them in 45 degree oven overnight and they still fall of. > > Any suggestions? > > Thanks. > > > ----------------------------------------------------------------- > THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED > AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE > ADDRESSEE LISTED ABOVE. > This record has been disclosed in accordance with Subtitle 3 of > Title 4 of the Health-General Article of the Annotated Code of > Maryland. Further disclosure of medical information contained > herein is prohibited. > If you are neither the intended recipient nor the individual > responsible for delivering this message to the intended > recipient, you are hereby notified that any disclosure of > patient information is strictly prohibited. > If you have received this email in error, immediately notify us > by telephone or return email. > ----------------------------------------------------------------- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ - This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ----------------------------------------------------------------- THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE ADDRESSEE LISTED ABOVE. This record has been disclosed in accordance with Subtitle 3 of Title 4 of the Health-General Article of the Annotated Code of Maryland. Further disclosure of medical information contained herein is prohibited. If you are neither the intended recipient nor the individual responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure of patient information is strictly prohibited. If you have received this email in error, immediately notify us by telephone or return email. ----------------------------------------------------------------- ------------------------------ Message: 23 Date: Thu, 20 Dec 2007 17:09:28 -0600 From: Annette Hall Subject: RE: [Histonet] IHC for VIAS To: 'Patti Loykasek' , Godfrey Guerzon , "histonet@lists.utsouthwestern.edu" Message-ID: <71320B4EBC7C15419563EAFBFCD924651C7BD48A33@hades.pa-ucl.com> Content-Type: text/plain; charset="us-ascii" We had the same problem with our XT. We now put the slides at 60 degrees for 1-2 hours. It's not perfect but we retain most of the tissue. Annette J Hall, MT Histo Supervisor United Clinical Labs Dubuque, Ia -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Thursday, December 20, 2007 4:12 PM To: Godfrey Guerzon; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC for VIAS I would recommend some time in a 60-65 degree oven even if you do still want to leave them in a 37-45 degree oven overnight. That's just off the top of my head - busy day here. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > We are having problem with tissue falling off the slides when we run the > breast panel on the Ventana XT for examination with the VIAS instrument. > The PR, ER, Ki67 and Her2 tends to fall off. We use "plus" slides and > place them in 45 degree oven overnight and they still fall of. > > Any suggestions? > > Thanks. > > > ----------------------------------------------------------------- > THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED > AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE > ADDRESSEE LISTED ABOVE. > This record has been disclosed in accordance with Subtitle 3 of > Title 4 of the Health-General Article of the Annotated Code of > Maryland. Further disclosure of medical information contained > herein is prohibited. > If you are neither the intended recipient nor the individual > responsible for delivering this message to the intended > recipient, you are hereby notified that any disclosure of > patient information is strictly prohibited. > If you have received this email in error, immediately notify us > by telephone or return email. > ----------------------------------------------------------------- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ - This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 49, Issue 22 **************************************** From shive003 <@t> umn.edu Thu Jan 3 10:27:35 2008 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu Jan 3 10:27:57 2008 Subject: [Histonet] k-9 Vet Plasma Marker References: <200801031541.m03FfZZh002782@spamfilt.mfldclin.edu> Message-ID: <002001c84e25$8c78e9b0$b0065486@auxs.umn.edu> I use canine IgG, IgM, and IgA. Dilutions will vary, depending upon antibody source and stock immunoglobulin concentrations, plus whatever detection system, incubation times and temperatures that you use. Positive controls are dog tonsil for IgG and IgM; dog stomach or pylorus for IgA. Proteinase K enzyme digestion for 5'. Jan Shivers Senior Scientist Pathology Teaching Program Histology/IHC/EM Section Leader University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu ----- Original Message ----- From: "Bliven, Laura" To: ; Sent: Thursday, January 03, 2008 9:41 AM Subject: [Histonet] k-9 Vet Plasma Marker > What's the best plasma cell marker for tumors on dog tissue? > Additional info would be appreciated as to antigen retrieval and dilution, > even though we'll be testing out various steps. > Thanks, > Laura > > Laura Bliven > Histology Lab/IHC > Marshfield Laboratories > 1000 N. Oak Ave. > Marshfield, WI 54449 > > > > -------------------------------------------------------------------------------- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From godsgalnow <@t> aol.com Thu Jan 3 10:29:20 2008 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Thu Jan 3 10:29:50 2008 Subject: [Histonet] purchased tests Message-ID: <8CA1C3CED80AB1C-9D0-290@FWM-D18.sysops.aol.com> To all the labs out there that send out specimens for a purchased test, how do you disclose that on the final pathology report?? IS there a disclaimer that you sue to indicate that the work was performed someplace else? Roxanne Soto HT(ASCP)QIHC Supervisor, Molecular Diagnostics & Quality Systems PhysiciansRightPath Tampa, Florida 813-549-1050 rsoto@physiciansrightpath.com ________________________________________________________________________ More new features than ever. Check out the new AOL Mail ! - http://webmail.aol.com From BNuern <@t> coj.net Thu Jan 3 10:44:52 2008 From: BNuern <@t> coj.net (Nuernberger, Barb) Date: Thu Jan 3 10:45:14 2008 Subject: [Histonet] (no subject) Message-ID: <66529604C8644845BB24FF6F6235664101FCC270@EVS1.coj.net> Help, Leica 1150H embedding center not dispensing! Changed fuse but dispensing tube is cool enough to hold, doesn't feel warm enough to me. Forcep warmer is hot. Any ideas? Barb Nuernberger Chief Toxicologist Not a histologist but in charge of problem! Medical Examiner's Office 2100 Jefferson St. Jacksonville,FL 32206 (904)630-5470 BNuern@coj.net From rjbuesa <@t> yahoo.com Thu Jan 3 10:50:02 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 3 10:50:26 2008 Subject: [Histonet] purchased tests In-Reply-To: <8CA1C3CED80AB1C-9D0-290@FWM-D18.sysops.aol.com> Message-ID: <176513.6979.qm@web61211.mail.yahoo.com> Yes, the disclaimer has to be included for your own sake. We used to, not only say that, but to attach a copy of the report submitted by the testing site. Ren? J. godsgalnow@aol.com wrote: --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From rjbuesa <@t> yahoo.com Thu Jan 3 10:51:03 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 3 10:51:26 2008 Subject: [Histonet] (no subject) In-Reply-To: <66529604C8644845BB24FF6F6235664101FCC270@EVS1.coj.net> Message-ID: <526515.79354.qm@web61218.mail.yahoo.com> You will be much better off by contacting your Leica microsystems representative. Ren? J. "Nuernberger, Barb" wrote: --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From CBraaten <@t> Cheshire-Med.COM Thu Jan 3 11:00:54 2008 From: CBraaten <@t> Cheshire-Med.COM (Christine I. Braaten) Date: Thu Jan 3 11:01:18 2008 Subject: [Histonet] (no subject) Message-ID: Happy New Year histonet folks! We are in the market for a new IHC stainer in 2008 and are interested in one that has FISH/ISH capabilities and also HPV testing. Can anyone make a recommendation? We will be looking at Ventana's Benchmark XT and also Leica's Bond Maxx. Are there others that would perform all these tests? Thanks in advance for your help. Christine CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. From rjbuesa <@t> yahoo.com Thu Jan 3 11:03:52 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 3 11:04:16 2008 Subject: [Histonet] (no subject) In-Reply-To: Message-ID: <891587.6966.qm@web61211.mail.yahoo.com> Pay special attention to the BondMax, to me is the better of the twqo you are going to test. Ren? J. "Christine I. Braaten" wrote: --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From RSRICHMOND <@t> aol.com Thu Jan 3 11:12:51 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Thu Jan 3 11:13:14 2008 Subject: [Histonet] Re: Barr body in Cell/culture - chromatin differentiation Message-ID: Naira V. Margaryan, D.V.M., Ph.D. at Children's Memorial Research Center in Chicago asks: >>I need help with some technique. Do you know a method how to recognize Barr body in Cell/culture which is mean we need a method to stain chromatin to differentiate male from female?<< There is an old (1960's) staining method for Barr bodies (sex chromatin bodies) which was supposed to be fairly specific with a lot of adjustment. But do cells in tissue culture have Barr bodies? The Barr body is the inactivated X-chromosome. This inactivation (the Lyon phenomenon, after Mary Lyon who described it in the 1960's) occurs somewhere around day 15 of human embryogenesis. Not all human somatic cells have identifiable Barr bodies (squamous epithelium and smooth muscle do, liver and kidney don't). I would think that a more specific method such as fluorescent in situ hybridization (FISH) would be preferable. Bob Richmond Samurai Pathologist Knoxville TN From Joanne.Malinowski <@t> crl.com Thu Jan 3 12:38:33 2008 From: Joanne.Malinowski <@t> crl.com (Malinowski, Joanne O,) Date: Thu Jan 3 12:39:01 2008 Subject: [Histonet] PEEK and PLLA/PGA Devices Message-ID: <3E94391DD24ECE418DCBA5EF74BA29943E83EC@shr-exch1.na01.crl.com> Happy New Year Histonetters! Can anyone share with me any experience they might have had with polyetheretherketone (PEEK) and PLLA/PGA? Specifically cutting bone containing this in paraffin. I'd appreciate any help with this. Thank you. Joanne Malinowski,HT ASCP Plastics Lab Manager, Medical Devices Division Charles River Laboratories Pathology Associates 15 Worman's Mill Court, Suite I Frederick, Maryland 21701 Phone 301-624-2034 Fax 301-663-8994 Email: joanne.malinowski@us.crl.com From emerald_lake77 <@t> yahoo.com Thu Jan 3 13:11:10 2008 From: emerald_lake77 <@t> yahoo.com (GT Hebert) Date: Thu Jan 3 13:11:31 2008 Subject: [Histonet] VIMENTIN - does it stain monocytes/macrophages? In-Reply-To: Message-ID: <261531.8820.qm@web31710.mail.mud.yahoo.com> Hello, I was just requested by my supervisor to ask the histonet the following question: Can a antibody against Vimentin stain monocytes/macrophages? We are working with human heart / coronary artery samples. I do not have much more information than that... but I figured anyone who deals with this protein would have considerable knowledge to quickly answer this question. Thank you in advance for your assistance. Sincerely, Gustave Hebert Scientist II Wyeth Research Cambridge MA --------------------------------- Never miss a thing. Make Yahoo your homepage. From gayle.callis <@t> bresnan.net Thu Jan 3 13:12:02 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Thu Jan 3 13:12:25 2008 Subject: [Histonet] late reply on softening hooves Message-ID: <005501c84e3c$85d8c780$6501a8c0@DHXTS541> A late reply. An excellent method for softening hooves is found in Histo Logic (Sakura Finetek website) and written by Jane Chladny several years ago. Animal hooves are particularly diffiicult and she had a good method for doing horse and bovine hooves. Check the Histo Logic topics with key words, or author's name to access the protocol. Gayle M. Callis HT/HTL//MT(ASCP) Bozeman MT From Rcartun <@t> harthosp.org Thu Jan 3 13:43:26 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Jan 3 13:44:02 2008 Subject: [Histonet] VIMENTIN - does it stain monocytes/macrophages? In-Reply-To: <261531.8820.qm@web31710.mail.mud.yahoo.com> References: <261531.8820.qm@web31710.mail.mud.yahoo.com> Message-ID: <477CF48F0200007700009F90@gwmail4.harthosp.org> Yes, vimentin is the intermediate filament found in monocytes/macrophages. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> GT Hebert 01/03/08 2:11 PM >>> Hello, I was just requested by my supervisor to ask the histonet the following question: Can a antibody against Vimentin stain monocytes/macrophages? We are working with human heart / coronary artery samples. I do not have much more information than that... but I figured anyone who deals with this protein would have considerable knowledge to quickly answer this question. Thank you in advance for your assistance. Sincerely, Gustave Hebert Scientist II Wyeth Research Cambridge MA --------------------------------- Never miss a thing. Make Yahoo your homepage. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From rgarhart <@t> system1.net Thu Jan 3 13:45:47 2008 From: rgarhart <@t> system1.net (Robert Garhart) Date: Thu Jan 3 13:45:39 2008 Subject: [Histonet] HT and HTL Salary Questionaire In-Reply-To: <000201c77d29$5ef145f0$800aa8c0@domain.local> References: <000201c77d29$5ef145f0$800aa8c0@domain.local> Message-ID: Histonetters, I am putting together some info to share with those in the HT and HTL community but plan to keep all individual information confidential. I was hoping that many of you could supply some info on the following few questions. 1. Are you HT or HTL? Bench/Supervisor/Manager? 2. What region of the country or state are you located? 3. How many years of experience do you have? 4. What is your salary or hourly wage? Do you make overtime? How much? 5. Do you feel that your wage is average, above average, or below average? Again, this is to supply to many of groups that I consult with and individual information will be kept confidential. I simply want to be able to be a better guide to those groups as to the going rate within the HT/HTL community at this time. The intent is to make sure that when opportunities arise for change that the salaries provide adequate opportunity to move forward. I appreciate your responses and look forward to having this information to compile. Best Regards, Robert Garhart Executive Recruiter System 1 Search 678-342-9029 Office rgarhart@system1.net Website: www.system1.net Please Join my Network on Linked In http://www.linkedin.com/in/robertgarhart From jfish <@t> gladstone.ucsf.edu Thu Jan 3 14:26:21 2008 From: jfish <@t> gladstone.ucsf.edu (Jo Dee Fish) Date: Thu Jan 3 14:26:48 2008 Subject: [Histonet] Pressure cooker question Message-ID: <000001c84e46$e7861320$2e0d010a@JFISH> We are searching for a cheap but reliable way to do antigen retrieval. Is anyone out there using an electric pressure cooker? And, are the ordinary household appliances working for you? Thanks in advance for your help. Jo Dee ~~Jo Dee Fish~~ Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 From mwich <@t> 7thwavelabs.com Thu Jan 3 14:27:50 2008 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Thu Jan 3 14:28:12 2008 Subject: [Histonet] immunocal Message-ID: <2264717ADC396742A0FF0AAB674F9A0D486B79@7THWAVE-SERVER.7thwave.local> Is anyone out there using Immunocal as a decalcifying agent for large animal bones such as goat or sheep? If so, can you give me some idea as to volume ratios, duration and the frequency at which the Immunocal must be changed? Thank you much! This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From mcauliff <@t> umdnj.edu Thu Jan 3 15:00:56 2008 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Jan 3 15:01:26 2008 Subject: [Histonet] VIMENTIN - does it stain monocytes/macrophages? In-Reply-To: <261531.8820.qm@web31710.mail.mud.yahoo.com> References: <261531.8820.qm@web31710.mail.mud.yahoo.com> Message-ID: <477D4D08.6040104@umdnj.edu> While vimentin may stain them it is not specific for the monocyte/macrophage line. Geoff GT Hebert wrote: > Hello, > > I was just requested by my supervisor to ask the histonet the following question: > > Can a antibody against Vimentin stain monocytes/macrophages? > > We are working with human heart / coronary artery samples. I do not have much more information than that... but I figured anyone who deals with this protein would have considerable knowledge to quickly answer this question. > > Thank you in advance for your assistance. > > Sincerely, > > Gustave Hebert > Scientist II > Wyeth Research > Cambridge MA > > > --------------------------------- > Never miss a thing. Make Yahoo your homepage. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From igor.deyneko <@t> gmail.com Thu Jan 3 15:01:52 2008 From: igor.deyneko <@t> gmail.com (Igor Deyneko) Date: Thu Jan 3 15:02:17 2008 Subject: [Histonet] Brdu Message-ID: <35e16a770801031301h7eff9d22n74b330f5344b5aa7@mail.gmail.com> Dear Histonetters! I have a question about Brdu. I have never used it. My questions are as follows: 1) How should the tissues be processed , paraffin-embedded or frozen??? (Will be used with xenograft tumors) 2) If there are kits, which have you found give better results with minimal non-specific staining 3) If not , is there a general protocol for this type of procedure? Thank you for any help. Sincerely, Igor Deyneko Infinity Pharmaceuticals Cambridge, MA From sharon.willman <@t> bms.com Thu Jan 3 15:17:19 2008 From: sharon.willman <@t> bms.com (Sharon E Willman) Date: Thu Jan 3 15:17:42 2008 Subject: [Histonet] Lendrun and Fraser Stain Message-ID: <14792f149825.14982514792f@bms.com> Hi, I was wondeering if anyone has the protocol for the Lendrun stain and also the Fraser stain? I would appreciate your feedback. Thanks, Sharon From rjbuesa <@t> yahoo.com Thu Jan 3 15:27:13 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 3 15:27:37 2008 Subject: [Histonet] Lendrun and Fraser Stain In-Reply-To: <14792f149825.14982514792f@bms.com> Message-ID: <746225.66186.qm@web61218.mail.yahoo.com> Sharon: There is a Lendrum's stain with acid fuchsin (1945), for granules in breast and kidney (1945) and other two (phloxine-tartrazine (1947) and eosin Y-erythrosin-phloxine-tartrazine NS (1939). Which is the one you are looking for? Ren? J. Sharon E Willman wrote: --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From emerald_lake77 <@t> yahoo.com Thu Jan 3 15:39:48 2008 From: emerald_lake77 <@t> yahoo.com (GT Hebert) Date: Thu Jan 3 15:40:11 2008 Subject: [Histonet] VIMENTIN - does it stain monocytes/macrophages? In-Reply-To: <477D4D08.6040104@umdnj.edu> Message-ID: <719871.72723.qm@web31702.mail.mud.yahoo.com> Thank you all for your assistance. Gustave Hebert --------------------------------- Never miss a thing. Make Yahoo your homepage. From relia1 <@t> earthlink.net Thu Jan 3 15:43:08 2008 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Jan 3 15:43:35 2008 Subject: [Histonet] RE: HT and HTL "Salary Survey" posting Message-ID: Hi Histonetters! I hope everybody had a wonderful holiday season. I just read the posting from Robert Garhart a recruiter at System One Search. I just wanted to comment to those of you who might not be aware that by releasing this information you are actually releasing basic pre interview information. I am not saying don't do it I am just saying be aware of how the information could potentially be used. (i.e. Your information could be released to an employer for a potential job opening without your permission). By releasing this information you could be giving up your confidentiality. Having been a professional recruiter for over 25 years I have seen these types of requests before and more importantly the havoc it can wreak for an individual. Here are several scenarios I have seen: Each of these scenarios starts this way: The recruiter takes the information you have supplied for this "survey" and gives it to a client (a fishing expedition of sorts), without your knowledge or permission, then once there is interest from the client they contact you, regardless of whether or not you are looking for a job. Here are the potential outcomes: 1. You send your resume to a client and are not considered for the position because you have already been submitted by a recruiter without your knowledge or permission and the client is not paying fees at that time. 2. You are working with a recruiter who you have consented to have represent you at a client and that recruiter who you trust to represent you has accurately given you all the information on the position before submitting your resume and has done all of the footwork with the client is excluded from your placement because you have been submitted by another recruiter without your knowledge or permission. In this scenario you could also be cut from consideration because employers don't like to get caught up in conflicts between recruiters. Not to mention it makes you the candidate, look unprofessional by not realizing that someone you have not given permission to is representing you. 3. Your resume is sent to your current employer or an affiliate facility without your permission or knowledge - OUCH!!. 4. By giving this information you could be in violation of your company's own confidentialty policy. This information could also be sold for SPAM or used to build a candidate database. And as most of you know the information he is looking for is actually available in a confidential format free of charge at both the NSH and ASCP websites and at the website Salary.com I am not saying don't respond I am merely saying be aware of what could potentially result from responding. In this day and age it is very important to have control of your personal information, who you give it out to and how it is used. Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.myspace.com/pamatrelia From TJJ <@t> Stowers-Institute.org Thu Jan 3 16:13:03 2008 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Thu Jan 3 16:13:46 2008 Subject: [Histonet] Embedding E18.5 mouse embryos for vibrating microtome sectioning Message-ID: I have some formalin fixed E18.5 mouse embryos I need to do vibrating microtome (VT-1000S) sectioning at 200 microns thickness. I'm having a devil of a time getting proper support using 4% agarose. Can someone give me some advice on what else to try, perhaps using a gelatin (?%) post-fixed in formalin would provide better support? Those guys have very tough and waterproof skin, and solutions don't penetrate it well. I have bisected them and will start sectioning the cut surface first. Thanks for your help and advice! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From gayle.callis <@t> bresnan.net Thu Jan 3 16:19:51 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Thu Jan 3 16:20:16 2008 Subject: [Histonet] immunocal References: <2264717ADC396742A0FF0AAB674F9A0D486B79@7THWAVE-SERVER.7thwave.local> Message-ID: <001301c84e56$c2c19680$6501a8c0@DHXTS541> You can use any acid decalcifier for animal bone. If the Immunocal is primarily a formic acid mixture, it will be slower than using a hydrochloric acid decalcifier. Check the MSDS to see WHAT acid is being used. If you want to ensure the bones are decalcified, then do an endpoint test to know when the calcium is removed. If the bones are whole bones, then even goat and/or sheep will be very large. Hopefully and to speed up fixation first and then decalcification, the bones are reduced in size by cutting slabs or open windows in the bone. 3 mm to 1 cm thick slabs will decalcify much faster than a whole bone. One danger in working with whole bones or any large animal bone is fixation must be total, or the acid decalcifier will macerate the soft tissues and cells. This is a reason cutting open the bones and/or letting them fix in NBF for a week or more is important. If the bone is pink/reddish inside after you fix then slice open, then the fixation should continue longer, and before you immerse in decalcifier. The volume of decalcifier to bone we have always used is 20 to 1, and suspend the bones in the decalcifying solution to allow the fluid to surround all sample surfaces. If you do a chemical endpoint test, then changing the decalcifying solution daily is important, although there is a nice weight loss, weight gain endpoint test that we have used with great success after we (stupidly!) gave away our FAXITRON X RAY unit. X ray is the most sensitive endpoint determination, followed by chemical testing, and even the weight gain/weight loss method, cheap, easy and fast to perform. You only need a good balance that weighs in milligrams. I will be happy to supply this method. A duration will depend on the size of the bone and the type and concentration of the acid one uses. Hence, endpoint testing takes away so much of the guessing game on how long it takes to decalcify a bone since age, size, and species of animals all are factors in how long it takes to decalcify. You can use 15% formic acid providing the bone is totally fixed, and another good decalcifier is 4% hydrochloric acid/8% formic acid - one we have used with great success for huge sheep distal femur slabs. Endpoint testing was done daily, and we did not leave the bones in decalcifying solution over a weekend IF the endpoint was close to completion. Overexposure aka over decalcification is not a good thing for any bones, and staining will be terrible if this happens. Gayle M. Callis HT/HTL/MT(ASCP) Bozeman MT ----- Original Message ----- From: "Michele Wich" To: Sent: Thursday, January 03, 2008 1:27 PM Subject: [Histonet] immunocal Is anyone out there using Immunocal as a decalcifying agent for large animal bones such as goat or sheep? If so, can you give me some idea as to volume ratios, duration and the frequency at which the Immunocal must be changed? Thank you much! This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Jan 3 16:46:50 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Jan 3 16:47:12 2008 Subject: [Histonet] purchased tests References: <8CA1C3CED80AB1C-9D0-290@FWM-D18.sysops.aol.com> Message-ID: <004501c84e5a$882ad230$0302a8c0@yourxhtr8hvc4p> Roxanne, we put a note in the report stating that the case was sent for xyz testing and an addendum will follow. Then, when the results are received, the addendum is added with "test xyz was performed at the such and such lab with the following results": Just remember, any lab that you do business with, you need to maintain a list of their CLIA and CAP numbers for tracking purposes. This is a CAP requirement. Happy New Year y'all Joe ----- Original Message ----- From: To: Sent: Thursday, January 03, 2008 10:29 AM Subject: [Histonet] purchased tests > To all the labs out there that send out specimens for a purchased test, > how do you disclose that on the final pathology report?? IS there a > disclaimer that you sue to indicate that the work was performed someplace > else? > > > Roxanne Soto HT(ASCP)QIHC > > Supervisor, Molecular Diagnostics & Quality Systems > > PhysiciansRightPath > > Tampa, Florida > > 813-549-1050 > > rsoto@physiciansrightpath.com > > ________________________________________________________________________ > More new features than ever. Check out the new AOL Mail ! - > http://webmail.aol.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From llewllew <@t> shaw.ca Thu Jan 3 18:07:57 2008 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Thu Jan 3 18:10:17 2008 Subject: [Histonet] Lendrun and Fraser Stain References: <14792f149825.14982514792f@bms.com> Message-ID: <002401c84e65$dc8eab20$05024246@yourlk4rlmsu> Lendrum, Fraser, McFarlane, Slidders and others published several trichrome and yellowsolve methods, mostly for fibrin. Several of them are on StainsFile. Go to: http://stainsfile.info then select "Staining techniques", then select the letter "L" and scroll down to Lendrum. The stain you want may be the picro-mallory. Bryan Llewellyn ----- Original Message ----- From: "Sharon E Willman" To: Sent: Thursday, January 03, 2008 1:17 PM Subject: [Histonet] Lendrun and Fraser Stain > Hi, > I was wondeering if anyone has the protocol for the Lendrun stain and > also the Fraser stain? I would appreciate your feedback. > Thanks, > Sharon > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Thu Jan 3 18:25:07 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Thu Jan 3 18:25:32 2008 Subject: [Histonet] immunocal References: Message-ID: <002101c84e68$424e1d40$6501a8c0@DHXTS541> Jim, We did not buffer 15% formic acid v/v in distilled water. We have also used 10% formic acid v/v in distilled water. The HCl/formic acid mixture is not buffered either. and is a fast decalcifying solution, more so than 10% or 15% formic acid. The bone actually buffers itself to a certain extent since it contains calcium and other phosphate salts. We always make up decalcifying solutions with distilled water, and the stock formic acid we purchase is 90% formic acid, not 100% formic acid (although the latter is available from Sigma and other companies). If you calculate the true concentration of formic acid made up from the 90% formic acid stock solution, you will have slightly less than a true 15% formic acid component. Buffered formic acid, sometimes called acidic buffers, will have a concentration of approximately 4.5%. Curiosity about formic acid concentration drove me to calculate the concentration - revealing a mild decalcifier with buffering salt that is excellent for immunohistochemical purposes. Caveat: one can still overexpose the bone to acid with these buffered acids. Many commercial buffered formic acid decalcifiers are made up from original, old recipes, i.e. sodium formate with formic acid and/or sodium citrate/formic acid. It is a good idea to read the MSDS to see content and hopefully, concentrations. You can find these mixtures in histotechnology textbooks, one is Kristensen's acid decalcifier, a classic. We rarely bought it commercially, choosing to make it up when needed. Convenience is everything in a busy laboratory though. Linda Jenkins had another excellent endpoint test that relied on viewing the sample, it was much simpler than weighing, xraying or chemical testing, and was accurate. Hopefully she will provide the method, although I have it buried in a file somewhere. Gayle M. Callis HT/HTL/MT(ASCP) Bozeman MT ----- Original Message ----- From: jstaruk To: 'Gayle Callis' Sent: Thursday, January 03, 2008 4:00 PM Subject: RE: [Histonet] immunocal Hi Gayle, I also do a lot of veterinary decalcifying. When you use the 15% formic acid or the HCl/formic acid, is it buffered with sodium citrate or do you make it in distilled water (i.e. 15ml formic acid into 85ml DH2O)? Thanks Jim _____________________ Jim Staruk Mass Histology Service www.masshistology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Thursday, January 03, 2008 5:20 PM To: Michele Wich; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] immunocal (snip) You can use 15% formic acid providing the bone is totally fixed, and another good decalcifier is 4% hydrochloric acid/8% formic acid - one we have used with great success for huge sheep distal femur slabs. Endpoint testing was done daily, and we did not leave the bones in decalcifying solution over a weekend IF the endpoint was close to completion. Overexposure aka over decalcification is not a good thing for any bones, and staining will be terrible if this happens. Gayle M. Callis HT/HTL/MT(ASCP) Bozeman MT From TMcNemar <@t> lmhealth.org Fri Jan 4 06:42:52 2008 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Jan 4 06:41:45 2008 Subject: [Histonet] Pressure cooker question In-Reply-To: <000001c84e46$e7861320$2e0d010a@JFISH> Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F512@lmhsmail.lmhealth.org> I used to use a pressure cooker but found it to be very harsh. I had a lot of trouble getting the sections to stay on the slide. For the last several years I'v use a $40 Black and Decker rice steamer. Slower than the pressure cooker but it always works and no problems with the sections. You might also look into microwave retrieval. I tried it a few times and it worked fine. I steam them for 20 minutes with a 20 minute cooling afterward. That gives me plenty of time to make up my buffer and set up my run. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jo Dee Fish Sent: Thursday, January 03, 2008 3:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pressure cooker question We are searching for a cheap but reliable way to do antigen retrieval. Is anyone out there using an electric pressure cooker? And, are the ordinary household appliances working for you? Thanks in advance for your help. Jo Dee ~~Jo Dee Fish~~ Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Fri Jan 4 07:54:19 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Jan 4 07:54:52 2008 Subject: [Histonet] Pressure cooker question In-Reply-To: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F512@lmhsmail.lmhealth.org> Message-ID: <000b01c84ed9$50ce3460$d00f7ca5@lurie.northwestern.edu> We use the Biocare decloaker for antigen retrieval. It is a pressure cooker and specifically programmed. Our sections stay on. We like to cook the slides overnight. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Friday, January 04, 2008 6:43 AM To: jfish@gladstone.ucsf.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Pressure cooker question I used to use a pressure cooker but found it to be very harsh. I had a lot of trouble getting the sections to stay on the slide. For the last several years I'v use a $40 Black and Decker rice steamer. Slower than the pressure cooker but it always works and no problems with the sections. You might also look into microwave retrieval. I tried it a few times and it worked fine. I steam them for 20 minutes with a 20 minute cooling afterward. That gives me plenty of time to make up my buffer and set up my run. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jo Dee Fish Sent: Thursday, January 03, 2008 3:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pressure cooker question We are searching for a cheap but reliable way to do antigen retrieval. Is anyone out there using an electric pressure cooker? And, are the ordinary household appliances working for you? Thanks in advance for your help. Jo Dee ~~Jo Dee Fish~~ Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Fri Jan 4 08:27:55 2008 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Jan 4 08:26:48 2008 Subject: [Histonet] Pressure cooker question In-Reply-To: <000b01c84ed9$50ce3460$d00f7ca5@lurie.northwestern.edu> Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F513@lmhsmail.lmhealth.org> Interesting. I used the Biocare decloaker for 5 minutes and charged slides. My tissues came off at least 50% of the time. Do you let your slides dry overnight before running them? Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: Bernice Frederick [mailto:b-frederick@northwestern.edu] Sent: Friday, January 04, 2008 8:54 AM To: Tom McNemar; jfish@gladstone.ucsf.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Pressure cooker question We use the Biocare decloaker for antigen retrieval. It is a pressure cooker and specifically programmed. Our sections stay on. We like to cook the slides overnight. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Friday, January 04, 2008 6:43 AM To: jfish@gladstone.ucsf.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Pressure cooker question I used to use a pressure cooker but found it to be very harsh. I had a lot of trouble getting the sections to stay on the slide. For the last several years I'v use a $40 Black and Decker rice steamer. Slower than the pressure cooker but it always works and no problems with the sections. You might also look into microwave retrieval. I tried it a few times and it worked fine. I steam them for 20 minutes with a 20 minute cooling afterward. That gives me plenty of time to make up my buffer and set up my run. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jo Dee Fish Sent: Thursday, January 03, 2008 3:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pressure cooker question We are searching for a cheap but reliable way to do antigen retrieval. Is anyone out there using an electric pressure cooker? And, are the ordinary household appliances working for you? Thanks in advance for your help. Jo Dee ~~Jo Dee Fish~~ Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JimR0712 <@t> comcast.net Fri Jan 4 08:37:23 2008 From: JimR0712 <@t> comcast.net (JimR0712@comcast.net) Date: Fri Jan 4 08:37:48 2008 Subject: [Histonet] Billing for ER, PR, Her2/Neu Message-ID: <010420081437.13147.477E44A30005E5B20000335B2215575474CDCEC9CFAD0307B6@comcast.net> We have been discussing which cpt code is appropriate for the IHC for Er, PR, and Her2/Neu. Currently we bil them as 88342 but some of the pathologists feel that we should bill the stains as 88361. Look forward to your valued input. Thanks. Jim. From Rcartun <@t> harthosp.org Fri Jan 4 09:37:26 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Jan 4 09:38:01 2008 Subject: [Histonet] Billing for ER, PR, Her2/Neu In-Reply-To: <010420081437.13147.477E44A30005E5B20000335B2215575474CDCEC9CFAD0307B6@comcast.net> References: <010420081437.13147.477E44A30005E5B20000335B2215575474CDCEC9CFAD0307B6@comcast.net> Message-ID: <477E0C660200007700009FA7@gwmail4.harthosp.org> It all depends on how your pathologists interpret them. We use Dr. Allred's semi-quantitative scoring system for ER/PR so we use 88360. We also use 88360 for HER2 based on the scoring system of 0, 1+, 2+, and 3+. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> 01/04/08 9:37 AM >>> We have been discussing which cpt code is appropriate for the IHC for Er, PR, and Her2/Neu. Currently we bil them as 88342 but some of the pathologists feel that we should bill the stains as 88361. Look forward to your valued input. Thanks. Jim. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From froyer <@t> bitstream.net Fri Jan 4 11:01:33 2008 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Jan 4 11:01:59 2008 Subject: [Histonet] Rotary Processor In-Reply-To: <582736990712161407u40e49110wc78c6df993e1d72@mail.gmail.com> References: <582736990712161407u40e49110wc78c6df993e1d72@mail.gmail.com> Message-ID: <007401c84ef3$76066850$7701a80a@Ford> I am posting this for a university researcher that needs a small processor to do a very low volume (5-10 blocks/month) for a research project. I suggested a rotary processor. If anyone out there has a surplus unit that they would like to sell, let me know. Example: Shandon Citadel, Leica TP 1020, Tissue-Tek II, etc. Thanks, ~ Ford Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL:? 612-839-1046 Phone:? 763-542-8725 Toll Free: 888-790-9686 From rjbuesa <@t> yahoo.com Fri Jan 4 11:08:05 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 4 11:08:30 2008 Subject: [Histonet] Rotary Processor In-Reply-To: <007401c84ef3$76066850$7701a80a@Ford> Message-ID: <688569.78108.qm@web61223.mail.yahoo.com> Look in eBay, they have some used instruments addresses and prices. Ren? J. Ford Royer wrote: --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From KMisono <@t> umaryland.edu Fri Jan 4 11:12:08 2008 From: KMisono <@t> umaryland.edu (Misono, Kaori) Date: Fri Jan 4 11:12:30 2008 Subject: [Histonet] Normal Goat Serum for IHC Message-ID: <3BBD3EDE51DB2043ABF3E819B1CD532A02994D58@cits-exch1.campus.umaryland.edu> Dear Histonetter, I am a reserach technician in the lab. I use Normal Goat Serum (NGS) for immunofluorescent IHC stainig as a blocking (10%). Recently I got a new batch of NGS and used it. Even my reliable primary antibody was negative on the rat brain tissue. I tested old vs new NGS, and old one worked. Does this happen a lot? Could someone know about good NGS supplier for the staining? Thank you for your help. Kaori From jmahoney <@t> alegent.org Fri Jan 4 11:18:55 2008 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Fri Jan 4 11:19:27 2008 Subject: [Histonet] Billing for ER, PR, Her2/Neu In-Reply-To: <010420081437.13147.477E44A30005E5B20000335B2215575474CDCEC9CFAD0307B6@comcast.net> References: <010420081437.13147.477E44A30005E5B20000335B2215575474CDCEC9CFAD0307B6@comcast.net> Message-ID: <477E161F0200003C00026697@gwia.alegent.org> You may only code 88361 if you are performing computer assisted analysis of the ER, PR and her-2. You should use 88360 of you are quantifying manually. Jan Mahoney, Alegent Health Omaha, NE From talulahgosh <@t> gmail.com Fri Jan 4 11:20:50 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Jan 4 11:21:12 2008 Subject: [Histonet] Normal Goat Serum for IHC In-Reply-To: <3BBD3EDE51DB2043ABF3E819B1CD532A02994D58@cits-exch1.campus.umaryland.edu> References: <3BBD3EDE51DB2043ABF3E819B1CD532A02994D58@cits-exch1.campus.umaryland.edu> Message-ID: We use serum for IHC from Equitech Bio. They have a minimum order of $50 but their serum is about $40 for 500 ml--cheap and reliable. Emily -- "Prosperity ripened the principle of decay...and, as soon as time or accident and removed the artificial supports, the stupendous fabric yielded to the pressure of its own weight...instead of inquiring why the Roman Empire was destroyed we should rather be surprised that it has subsisted for so long" -Edward Gibbon, Decline and Fall of the Roman Empire From RSRICHMOND <@t> aol.com Fri Jan 4 11:50:03 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Fri Jan 4 11:50:26 2008 Subject: [Histonet] Re: Lendrum stains Message-ID: Sharon E. Willman (where?) asks for information about "Lendrun" and Fraser stains, she doesn't say for what. A.C. Lendrum, I was told back around 1970, was one of the last histologists who understood textile dyes. Apparently there were a lot of small factories making obscure dyes in Scotland back then, and he knew all of these single malt dye-makers and collected numerous samples. His eclectic habits make it difficult to identify the dyes he used, let alone obtain and use them. That and the invention of immunohistochemistry probably consign most of his work to oblivion. Lendrum's "Obadiah" stain (he was fond of such fanciful names) for fibrin was in use in a research lab at Cornell Medical Center on east 68th street in New York City when I was there briefly in 1968. Stainsfile gives the following reference: Lendrum, A. C., et. al. (1962) "Studies on the character and staining of fibrin." Journal of Clinical Pathology, v. 15, p. 401. [this is a British journal, not the AJCP]. Rotsa ruck finding naphthalene blue black CS, Chicago red, or polar brilliant red BN. John Kiernan - Dick Dapson - Mike Titford - other geezers on this list - do you know anything more about A.C. Lendrum? He must have been an interesting guy! Bob Richmond Samurai Pathologist Knoxville TN From Sharon.Cook <@t> osumc.edu Fri Jan 4 12:04:37 2008 From: Sharon.Cook <@t> osumc.edu (Cook, Sharon ) Date: Fri Jan 4 12:05:02 2008 Subject: [Histonet] RE: Histonet Digest, Vol 50, Issue 5 In-Reply-To: References: Message-ID: <7A541592F9A53A4E86E7A3D5C4C7F68C01D7962B@msxc01.osumc.edu> I've unsubscribed 2 times and I continue to receive these emails. Please unsubscribe for me. Thanks, Sharon S. Cook, MT(ASCP) SBB Operations Director, Anatomic Pathology The Ohio State University E407 Doan Hall 410 West 10th Avenue Columbus, OH 43210 sharon.cook@osumc.edu Phone: 614 / 293-8418 Fax: 614 / 293-2779 Pager: 614 / 346-0746 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, January 04, 2008 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 50, Issue 5 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Lendrum stains (Robert Richmond) ---------------------------------------------------------------------- Message: 1 Date: Fri, 4 Jan 2008 12:50:03 -0500 From: "Robert Richmond" Subject: [Histonet] Re: Lendrum stains To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Sharon E. Willman (where?) asks for information about "Lendrun" and Fraser stains, she doesn't say for what. A.C. Lendrum, I was told back around 1970, was one of the last histologists who understood textile dyes. Apparently there were a lot of small factories making obscure dyes in Scotland back then, and he knew all of these single malt dye-makers and collected numerous samples. His eclectic habits make it difficult to identify the dyes he used, let alone obtain and use them. That and the invention of immunohistochemistry probably consign most of his work to oblivion. Lendrum's "Obadiah" stain (he was fond of such fanciful names) for fibrin was in use in a research lab at Cornell Medical Center on east 68th street in New York City when I was there briefly in 1968. Stainsfile gives the following reference: Lendrum, A. C., et. al. (1962) "Studies on the character and staining of fibrin." Journal of Clinical Pathology, v. 15, p. 401. [this is a British journal, not the AJCP]. Rotsa ruck finding naphthalene blue black CS, Chicago red, or polar brilliant red BN. John Kiernan - Dick Dapson - Mike Titford - other geezers on this list - do you know anything more about A.C. Lendrum? He must have been an interesting guy! Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 50, Issue 5 *************************************** From kmerriam2003 <@t> yahoo.com Fri Jan 4 12:21:23 2008 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Fri Jan 4 12:21:46 2008 Subject: [Histonet] RE: HT and HTL "Salary Survey" posting Message-ID: <564776.10450.qm@web50306.mail.re2.yahoo.com> Thanks for the tip Pam. I have actually had #3 happen to me (about 10 years ago or so): I had been at my new job for about 3 months and I got a call from someone in HR telling me that she had just received my resume from a recruiter. I had to explain to her that, obviously, I had been looking for a position prior to my arrival at that company so it must have been a left-over, but it was nevertheless an embarrassing moment for me (she was less than understanding - "human" relations was not her strong point; not so good for someone working in HR). I called the recruiter and gave him a piece of my mind!! He should not have sent my resume to anyone without my permission. Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: Pam Barker To: Histonet Sent: Thursday, January 3, 2008 4:43:08 PM Subject: [Histonet] RE: HT and HTL "Salary Survey" posting Hi Histonetters! I hope everybody had a wonderful holiday season. I just read the posting from Robert Garhart a recruiter at System One Search. I just wanted to comment to those of you who might not be aware that by releasing this information you are actually releasing basic pre interview information. I am not saying don't do it I am just saying be aware of how the information could potentially be used. (i.e. Your information could be released to an employer for a potential job opening without your permission). By releasing this information you could be giving up your confidentiality. Having been a professional recruiter for over 25 years I have seen these types of requests before and more importantly the havoc it can wreak for an individual. Here are several scenarios I have seen: Each of these scenarios starts this way: The recruiter takes the information you have supplied for this "survey" and gives it to a client (a fishing expedition of sorts), without your knowledge or permission, then once there is interest from the client they contact you, regardless of whether or not you are looking for a job. Here are the potential outcomes: 1. You send your resume to a client and are not considered for the position because you have already been submitted by a recruiter without your knowledge or permission and the client is not paying fees at that time. 2. You are working with a recruiter who you have consented to have represent you at a client and that recruiter who you trust to represent you has accurately given you all the information on the position before submitting your resume and has done all of the footwork with the client is excluded from your placement because you have been submitted by another recruiter without your knowledge or permission. In this scenario you could also be cut from consideration because employers don't like to get caught up in conflicts between recruiters. Not to mention it makes you the candidate, look unprofessional by not realizing that someone you have not given permission to is representing you. 3. Your resume is sent to your current employer or an affiliate facility without your permission or knowledge - OUCH!!. 4. By giving this information you could be in violation of your company's own confidentialty policy. This information could also be sold for SPAM or used to build a candidate database. And as most of you know the information he is looking for is actually available in a confidential format free of charge at both the NSH and ASCP websites and at the website Salary.com I am not saying don't respond I am merely saying be aware of what could potentially result from responding. In this day and age it is very important to have control of your personal information, who you give it out to and how it is used. Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.myspace.com/pamatrelia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ From nancy.troiano <@t> yale.edu Fri Jan 4 12:22:59 2008 From: nancy.troiano <@t> yale.edu (Nancy W. Troiano) Date: Fri Jan 4 12:23:29 2008 Subject: [Histonet] PEEK and PLLA/PGA Message-ID: <5.2.1.1.2.20080104132124.024477f0@email.med.yale.edu> Hi Joanne - we have cut blood vessels containing PLLA and PGA implants and use glycolmethacrylate since the PLLA and PGA can tear through paraffin. You may want to try GMA - we use the Technovit 7100 kit. From Kristopher.Kalleberg <@t> unilever.com Fri Jan 4 12:25:58 2008 From: Kristopher.Kalleberg <@t> unilever.com (Kalleberg, Kristopher) Date: Fri Jan 4 12:26:51 2008 Subject: [Histonet] Ki-67 Message-ID: <0E6BC087F70F9C47ACFF2C203D6E329C0376DE99@NTRSEVS30002.s3.ms.unilever.com> Hello All, I know most people have worked with the biomarker Ki-67. The question that has come about is why a few nuclei are staining in suprabasal layers of skin and not just specifically in the basal layer where they should be specifically assigned to. I am investigating both photoprotected sites of the arm along with solar lentigines (sun spots/freckles) of the arm. If anyone can help me answer this, I would greatly appreciate it. Thank you. Kris Kalleberg From jfish <@t> gladstone.ucsf.edu Fri Jan 4 12:34:10 2008 From: jfish <@t> gladstone.ucsf.edu (Jo Dee Fish) Date: Fri Jan 4 12:34:36 2008 Subject: [Histonet] Pressure cooker question In-Reply-To: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F513@lmhsmail.lmhealth.org> Message-ID: <000001c84f00$65c7db10$2e0d010a@JFISH> Hello everyone, I just want to thank all of those who responded to my pressure cooker question. I have decided to order a "3-in-1" cooker from Sears. It is able to be more flexible, it can either steam or pressure cook, as well as slow cook (which we won't be doing unless we make BBQ meatballs to go with our beer on Fridays!), so we can try different temperatures and times to optimize our protocol. I understand that the "scientific" pressure cookers are probably more accurate as far as temperature is concerned, but the price is prohibitive which is why I decided to buy the less expensive household version. Thanks for all of your help and advice, Jo Dee ~~Jo Dee Fish~~ Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 -----Original Message----- From: Tom McNemar [mailto:TMcNemar@lmhealth.org] Sent: Friday, January 04, 2008 6:28 AM To: Bernice Frederick; jfish@gladstone.ucsf.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Pressure cooker question Interesting. I used the Biocare decloaker for 5 minutes and charged slides. My tissues came off at least 50% of the time. Do you let your slides dry overnight before running them? Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: Bernice Frederick [mailto:b-frederick@northwestern.edu] Sent: Friday, January 04, 2008 8:54 AM To: Tom McNemar; jfish@gladstone.ucsf.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Pressure cooker question We use the Biocare decloaker for antigen retrieval. It is a pressure cooker and specifically programmed. Our sections stay on. We like to cook the slides overnight. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Friday, January 04, 2008 6:43 AM To: jfish@gladstone.ucsf.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Pressure cooker question I used to use a pressure cooker but found it to be very harsh. I had a lot of trouble getting the sections to stay on the slide. For the last several years I'v use a $40 Black and Decker rice steamer. Slower than the pressure cooker but it always works and no problems with the sections. You might also look into microwave retrieval. I tried it a few times and it worked fine. I steam them for 20 minutes with a 20 minute cooling afterward. That gives me plenty of time to make up my buffer and set up my run. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jo Dee Fish Sent: Thursday, January 03, 2008 3:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pressure cooker question We are searching for a cheap but reliable way to do antigen retrieval. Is anyone out there using an electric pressure cooker? And, are the ordinary household appliances working for you? Thanks in advance for your help. Jo Dee ~~Jo Dee Fish~~ Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Fri Jan 4 12:41:38 2008 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Fri Jan 4 12:42:07 2008 Subject: [Histonet] CJD Histology Procedures In-Reply-To: Message-ID: All, Could someone point me in the right direction for the most up-to-date CJD Procedures for the Histolab. Ours may be out of date and I need something to compare them too. Thanx In Advance, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI From llewllew <@t> shaw.ca Fri Jan 4 12:58:28 2008 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Fri Jan 4 13:21:22 2008 Subject: [Histonet] Re: Lendrum stains References: Message-ID: <000801c84f03$cb9ba310$05024246@yourlk4rlmsu> I trained in the UK during the early 1960s and went to Bromley Technical College every Friday night, a real pain since it was on the edge of South London and I lived on the edge of North London. The instructor was Wallington, of the text book, and he was regularly in contact with Lendrum. Even in those days (63 and 64) Lendrum was an exception among "dye tinkerers". Wallington told us that producing new methods for fibrin was pretty much a hobby for Lendrum. The one noted as the long method on StainsFile is actually from a variation given to Wallington by Lendrum earlier in that week of that Friday's lecture. I used to use all those methods when I was in Winnipeg during the 1970s, mostly on rejecting kidney transplants. Even then immunifluorescence was replacing staining methods for fibrin, certainly on renal biopsies. I always found the Obadiah an unsatisfactory stain, the Masson 44/41 too. The colours just didn't look right to me. The picro-mallory is, however, one of the stars in trichrome staining if done on properly prepared material. I always found it absolutely beautiful to look at, but then, I am noted as being a bit strange about things like that. I have substituted amido black 10B (naphthol blue black, CI # 20470) for naphthalene blue black CS, and it worked fine. They are structurally very similar. Bryan Llewellyn ----- Original Message ----- From: "Robert Richmond" To: Sent: Friday, January 04, 2008 9:50 AM Subject: [Histonet] Re: Lendrum stains > Sharon E. Willman (where?) asks for information about "Lendrun" and > Fraser stains, she doesn't say for what. > > A.C. Lendrum, I was told back around 1970, was one of the last > histologists who understood textile dyes. Apparently there were a lot > of small factories making obscure dyes in Scotland back then, and he > knew all of these single malt dye-makers and collected numerous > samples. His eclectic habits make it difficult to identify the dyes he > used, let alone obtain and use them. That and the invention of > immunohistochemistry probably consign most of his work to oblivion. > > Lendrum's "Obadiah" stain (he was fond of such fanciful names) for > fibrin was in use in a research lab at Cornell Medical Center on east > 68th street in New York City when I was there briefly in 1968. > Stainsfile gives the following reference: Lendrum, A. C., et. al. > (1962) "Studies on the character and staining of fibrin." Journal of > Clinical Pathology, v. 15, p. 401. [this is a British journal, not the > AJCP]. Rotsa ruck finding naphthalene blue black CS, Chicago red, or > polar brilliant red BN. > > John Kiernan - Dick Dapson - Mike Titford - other geezers on this list > - do you know anything more about A.C. Lendrum? He must have been an > interesting guy! > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Fri Jan 4 13:26:11 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Jan 4 13:26:37 2008 Subject: [Histonet] histochemistry an extinct art? Message-ID: <000501c84f07$aad39ee0$eeeea8c0@dielangs.at> Hi, I have a question for those, that are experienced with the old and the new techniques in histotechnology. I looked through some antiquariat-books, that deal a lot with histochemical demonstration of many tissue-compounds and enzymes. These techniques sound all very strange to me and it seems like a forgotten art. I work in a clinical histolab. Are the histochemical, enzymhistochemical methods still in use in research-labs? Did immunhistochemistry replace them all together? Is histochemistry a part of modern cellbiology or just obsolete? Gudrun Lang From Ronald.Houston <@t> nationwidechildrens.org Fri Jan 4 13:25:56 2008 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri Jan 4 13:26:53 2008 Subject: [Histonet] Re: Lendrum stains In-Reply-To: Message-ID: <979FF5962E234F45B06CF0DB7C1AABB2145D9FC8@chi2k3ms01.columbuschildrens.net> I fondly remember performing Lendrum's stains in a previous life back home in Scotland. The color contrasts of many of the techniques remains unsurpassed as far as tinctorial staining is concerned. Not so sure why none of the methods really took off in the States; perhaps you're right, Bob, it may have had to do with the fact that he got many of the dyes directly from the mills. Professor Alan C Lendrum was Professor of Pathology at both Glasgow and Dundee Universities (the latter until 1972 when I believe he retired). Perhaps John Bancroft, Peter Stoward and/or Richard Horobin would be able to comment more on Professor Lendrum, if they still subscribe to Histonet. It is certainly true that he was one of the last histopathologists who understood the properties of the dyes and the theory behind the techniques he introduced with his colleagues. His work was true Art in histology; alas a dieing trait (no pun intended). As far as I am concerned, his MSB (Martius Yellow, Crystal Scarlet, Soluble Blue) is still the best method for fibrin demonstration. Ronnie Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 Ronald.Houston@NationwideChildrens.org Columbus Children's Hospital is now Nationwide Children's Hospital www.NationwideChildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Friday, January 04, 2008 12:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Lendrum stains Sharon E. Willman (where?) asks for information about "Lendrun" and Fraser stains, she doesn't say for what. A.C. Lendrum, I was told back around 1970, was one of the last histologists who understood textile dyes. Apparently there were a lot of small factories making obscure dyes in Scotland back then, and he knew all of these single malt dye-makers and collected numerous samples. His eclectic habits make it difficult to identify the dyes he used, let alone obtain and use them. That and the invention of immunohistochemistry probably consign most of his work to oblivion. Lendrum's "Obadiah" stain (he was fond of such fanciful names) for fibrin was in use in a research lab at Cornell Medical Center on east 68th street in New York City when I was there briefly in 1968. Stainsfile gives the following reference: Lendrum, A. C., et. al. (1962) "Studies on the character and staining of fibrin." Journal of Clinical Pathology, v. 15, p. 401. [this is a British journal, not the AJCP]. Rotsa ruck finding naphthalene blue black CS, Chicago red, or polar brilliant red BN. John Kiernan - Dick Dapson - Mike Titford - other geezers on this list - do you know anything more about A.C. Lendrum? He must have been an interesting guy! Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From smehta <@t> magenbiosciences.com Fri Jan 4 13:34:25 2008 From: smehta <@t> magenbiosciences.com (Shanu Mehta) Date: Fri Jan 4 13:34:49 2008 Subject: [Histonet] Course in Histology Message-ID: <4C78CF3C61E6A640888226885709EFE9379DAB@server01.magen.local> Hello Histonetters, Is there a histology course I can take anywhere in the Boston area? I did not take any formal training/education in Histology but have been doing the same for almost 2 years now. It would be great if I could get some formal knowledge in Histology to help me understand the basic problems I face sometimes during my routine work. Thanks a lot for any help with this. Shanu ------------ Shanu Mehta Magen Biosciences 100 Beaver St.Suite 101 Waltham, MA 02453 Direct Dial: 781-314-2918 Fax: 781-314-2999 Main Line: 781-314-2900 Email: smehta@magenbiosciences.com http://www.magenbiosciences.com From Dorothy.L.Webb <@t> HealthPartners.Com Fri Jan 4 13:46:30 2008 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Fri Jan 4 13:46:55 2008 Subject: [Histonet] Sausage blocks Message-ID: <0E394B648E5284478A6CCB78E5AFDA2705635392@hpes1.HealthPartners.int> Does anyone use sausage blocks for their IHC controls? I have heard pros and cons to this control usage, the cons being mostly too time consuming to keep up. Would like some input as to what others are doing in this regard!! Thanks ahead of time!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From bdelescavage <@t> cellnetix.com Fri Jan 4 14:00:22 2008 From: bdelescavage <@t> cellnetix.com (Beth Delescavage) Date: Fri Jan 4 14:04:09 2008 Subject: [Histonet] Surfactant A protein Message-ID: Hi All~ Thermo/Neomarkers/ Lab Vision has discontinued the Surfactant A clone PE10 antibody we have been using and are not offering any replacements. Are any of you using the same clone from a different vendor and do you like the staining? Have a nice weekend! ~Beth Beth Delescavage, BS, HTL (ASCP) QIHC CellNetix Laboratories Histotechnologist DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From portera <@t> msu.edu Fri Jan 4 14:47:41 2008 From: portera <@t> msu.edu (Amy Porter) Date: Fri Jan 4 14:48:22 2008 Subject: [Histonet] CJD Histology Procedures References: Message-ID: <002001c84f13$0c9b33d0$8e7a0923@histolab> I would recommend the CDC website. Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 355-6475 ext 1480 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu ----- Original Message ----- From: "Dawson, Glen" To: Sent: Friday, January 04, 2008 1:41 PM Subject: [Histonet] CJD Histology Procedures All, Could someone point me in the right direction for the most up-to-date CJD Procedures for the Histolab. Ours may be out of date and I need something to compare them too. Thanx In Advance, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Gguerzon <@t> lifebridgehealth.org Fri Jan 4 15:07:23 2008 From: Gguerzon <@t> lifebridgehealth.org (Godfrey Guerzon) Date: Fri Jan 4 15:08:40 2008 Subject: [Histonet] Course in Histology Message-ID: There is a web course in Histotechnology at Harford Community College in Maryland. You may want to check it out. The Director of the program is Mr. Floyd Grimm but I believe he is retiring. His phone number is (410) 836-4372. His e-mail address fgrimm@harford.edu. You may also check the college website. Godfrey >>> "Shanu Mehta" 1/4/2008 2:34 PM >>> Hello Histonetters, Is there a histology course I can take anywhere in the Boston area? I did not take any formal training/education in Histology but have been doing the same for almost 2 years now. It would be great if I could get some formal knowledge in Histology to help me understand the basic problems I face sometimes during my routine work. Thanks a lot for any help with this. Shanu ------------ Shanu Mehta Magen Biosciences 100 Beaver St.Suite 101 Waltham, MA 02453 Direct Dial: 781-314-2918 Fax: 781-314-2999 Main Line: 781-314-2900 Email: smehta@magenbiosciences.com http://www.magenbiosciences.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------------------------------- THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE ADDRESSEE LISTED ABOVE. This record has been disclosed in accordance with Subtitle 3 of Title 4 of the Health-General Article of the Annotated Code of Maryland. Further disclosure of medical information contained herein is prohibited. If you are neither the intended recipient nor the individual responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure of patient information is strictly prohibited. If you have received this email in error, immediately notify us by telephone or return email. ----------------------------------------------------------------- From talulahgosh <@t> gmail.com Fri Jan 4 15:19:00 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Jan 4 15:19:24 2008 Subject: [Histonet] jackson immuno backorder Message-ID: Has anyone had Jackson Immunoresearch say their secondary antibodies are on backorder indefinitely? Another lab was told it would be an indefinite amount of time before they could get them. Emily -- "Prosperity ripened the principle of decay...and, as soon as time or accident and removed the artificial supports, the stupendous fabric yielded to the pressure of its own weight...instead of inquiring why the Roman Empire was destroyed we should rather be surprised that it has subsisted for so long" -Edward Gibbon, Decline and Fall of the Roman Empire From llewllew <@t> shaw.ca Fri Jan 4 14:57:11 2008 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Fri Jan 4 15:21:37 2008 Subject: [Histonet] histochemistry an extinct art? References: <000501c84f07$aad39ee0$eeeea8c0@dielangs.at> Message-ID: <002801c84f14$604e65f0$05024246@yourlk4rlmsu> Immunohistochemistry is certainly the latest technique to coma along, and is undoubtedly of very great value. It is now in its infancy, I suspect, and I fully expect that IHC procedures will increase in usefulness as time passes. Probably some antibodies will stop being used as clinical correlation with specific antobodies becomes more standardised. However, the commonest method used in histolabs today is about 120 years old, or more. That is the H&E. There is no sign that it is going into "that long night". Much the same can be said for Masson's trichrome and its clones, the PAS histochemical procedure, Perls' histochemical procedure. How do you demonstrate reticulin, or elastic? Enzyme histochemistry is still used to demonstrate muscle fibre types. The list could go on, but I am of the opinion that the older methods still have great value and will continue to have great value for a long time to come. Sometimes they are far more convenient than IHC (toluidine blue for helicobacter, for instance), even when IHC is pushed for it. Part of the reason is that IHC and enzyme histochemistry, for instance, demonstrate different things. If an antibody against alkaline phosphatase is used, you will demonstrate the enzyme, i.e. the protein. If you do an alkaline phosphatase stain using naphthol phosphate and a diazonium salt, you demonstrate the activity of the enzyme. Those are two different things. I know I am a bit of an old fogey on this, but I always strongly recommend that histotechs become conversant with the whole range of procedures used histologically, dye staining, histochemistry and IHC. Bryan Llewellyn ----- Original Message ----- From: "Gudrun Lang" To: Sent: Friday, January 04, 2008 11:26 AM Subject: [Histonet] histochemistry an extinct art? > Hi, > > I have a question for those, that are experienced with the old and the new > techniques in histotechnology. I looked through some antiquariat-books, > that > deal a lot with histochemical demonstration of many tissue-compounds and > enzymes. These techniques sound all very strange to me and it seems like a > forgotten art. > > I work in a clinical histolab. Are the histochemical, enzymhistochemical > methods still in use in research-labs? Did immunhistochemistry replace > them > all together? Is histochemistry a part of modern cellbiology or just > obsolete? > > > > Gudrun Lang > > -------------------------------------------------------------------------------- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Fri Jan 4 15:26:13 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 4 15:26:37 2008 Subject: [Histonet] histochemistry an extinct art? In-Reply-To: <002801c84f14$604e65f0$05024246@yourlk4rlmsu> Message-ID: <430699.41709.qm@web61220.mail.yahoo.com> Amen to all that from the bottom of my very old heart! Ren? Bryan Llewellyn wrote: Immunohistochemistry is certainly the latest technique to coma along, and is undoubtedly of very great value. It is now in its infancy, I suspect, and I fully expect that IHC procedures will increase in usefulness as time passes. Probably some antibodies will stop being used as clinical correlation with specific antobodies becomes more standardised. However, the commonest method used in histolabs today is about 120 years old, or more. That is the H&E. There is no sign that it is going into "that long night". Much the same can be said for Masson's trichrome and its clones, the PAS histochemical procedure, Perls' histochemical procedure. How do you demonstrate reticulin, or elastic? Enzyme histochemistry is still used to demonstrate muscle fibre types. The list could go on, but I am of the opinion that the older methods still have great value and will continue to have great value for a long time to come. Sometimes they are far more convenient than IHC (toluidine blue for helicobacter, for instance), even when IHC is pushed for it. Part of the reason is that IHC and enzyme histochemistry, for instance, demonstrate different things. If an antibody against alkaline phosphatase is used, you will demonstrate the enzyme, i.e. the protein. If you do an alkaline phosphatase stain using naphthol phosphate and a diazonium salt, you demonstrate the activity of the enzyme. Those are two different things. I know I am a bit of an old fogey on this, but I always strongly recommend that histotechs become conversant with the whole range of procedures used histologically, dye staining, histochemistry and IHC. Bryan Llewellyn ----- Original Message ----- From: "Gudrun Lang" To: Sent: Friday, January 04, 2008 11:26 AM Subject: [Histonet] histochemistry an extinct art? > Hi, > > I have a question for those, that are experienced with the old and the new > techniques in histotechnology. I looked through some antiquariat-books, > that > deal a lot with histochemical demonstration of many tissue-compounds and > enzymes. These techniques sound all very strange to me and it seems like a > forgotten art. > > I work in a clinical histolab. Are the histochemical, enzymhistochemical > methods still in use in research-labs? Did immunhistochemistry replace > them > all together? Is histochemistry a part of modern cellbiology or just > obsolete? > > > > Gudrun Lang > > -------------------------------------------------------------------------------- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From disbrc <@t> shands.ufl.edu Fri Jan 4 20:35:58 2008 From: disbrc <@t> shands.ufl.edu (Carrie Disbrow) Date: Fri Jan 4 20:36:32 2008 Subject: [Histonet] on call coverage Message-ID: <477EA6BE.72AC.0059.0@shands.ufl.edu> We think our hourly pay to carry the beeper when on call is too low and we have talked to management. We supply coverage on weekends and holidays and we rotate the beeper weekly. Our employer said if we took a survey and the results revealed our on-call pay is below average we might get an increase. Would anyone like to participate in our survey? From JWEEMS <@t> sjha.org Sat Jan 5 07:39:14 2008 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Sat Jan 5 07:41:24 2008 Subject: [Histonet] on call coverage References: <477EA6BE.72AC.0059.0@shands.ufl.edu> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3204726B4B@sjhaexc02.sjha.org> Ours is 3.50/hr - Sundays and holidays. If called back, we have a "call back" clock code, which allows extra for travel. Good luck! j Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Carrie Disbrow Sent: Fri 1/4/2008 9:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] on call coverage We think our hourly pay to carry the beeper when on call is too low and we have talked to management. We supply coverage on weekends and holidays and we rotate the beeper weekly. Our employer said if we took a survey and the results revealed our on-call pay is below average we might get an increase. Would anyone like to participate in our survey? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maxim_71 <@t> mail.ru Sat Jan 5 07:43:38 2008 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Sat Jan 5 07:45:37 2008 Subject: [Histonet] immunocal Message-ID: <1761665308.20080105164338@mail.ru> Gayle: Does this solution (4% hydrochloric acid/8% formic acid) damage to IHC in any ways or better use 15% formic acid for this purpose? We will do IHC for breast (ER, PgR, Ki67 and Her2). Maxim Peshkov Taganrog, Russia. From JWEEMS <@t> sjha.org Sat Jan 5 10:30:44 2008 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Sat Jan 5 10:30:35 2008 Subject: [Histonet] immunocal References: <1761665308.20080105164338@mail.ru> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3204726B4C@sjhaexc02.sjha.org> Maxim, If Gayle's answer is that your solution does not work well for IHC, there is a product from BBC Biochemical that is designed for IHC - and it does work well. We use it for our bonemarrows. http://www.bbcus.com 6086 RapidCal?Immuno?, 1Pint 6087 RapidCal?Immuno?, 1 Quart 6089 RapidCal?Immuno?, 1 Gallon 6090 RapidCal?Immuno?, 1 Gallon Cube Cheers! j Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Maxim Peshkov Sent: Sat 1/5/2008 8:43 AM To: Gayle Callis Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] immunocal Gayle: Does this solution (4% hydrochloric acid/8% formic acid) damage to IHC in any ways or better use 15% formic acid for this purpose? We will do IHC for breast (ER, PgR, Ki67 and Her2). Maxim Peshkov Taganrog, Russia. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bakevictoria <@t> gmail.com Sat Jan 5 11:28:41 2008 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Sat Jan 5 11:29:10 2008 Subject: [Histonet] on call coverage In-Reply-To: <477EA6BE.72AC.0059.0@shands.ufl.edu> References: <477EA6BE.72AC.0059.0@shands.ufl.edu> Message-ID: <4f016b690801050928ke0a74acncb4a9f6744cca8e6@mail.gmail.com> Carrie, I've worked in different situations where on call was required and each handled it a bit differently. In one place we rotated on call/weekend service where we had a day off during the week, worked a FULL Saturday and took call for the next day. Our base was $25 for just carrying the beeper on Sunday a percentage for after hours coverage on Saturday. If we were called back in then we received automatic 2 hours OT, whether we were there for 15 minutes or the full 2 hours, any additional hours were covered at the time and a half rate. If it was a holiday and we were called back we were compensated at the holiday rate under the same guidelines. For weeknight coverage we were paid something like $2 - 3 per hour with the same OT as weekends if we were called back. This was between 1996-1999. In another position I was the lab manager and I was essentially on call 24/7 without any compensation - this meant if I was called late at night for a freezer alarm or an off hour tissue collection/animal take down, I had to come in. I was then given "compensatory time off" in lieu of $'s. In someways this worked out pretty well as I'm a single parent I used to use this time if I had to take off for a family reasons. In another lab, my staff was only on call for weekend/holiday emergency biopsy services - no frozens were done in our lab - and we usually knew on Friday night if we would have a case coming through that would require someone to come in. So coverage would begin on Saturday morning at 8am and end at 6pm Sunday night. Each tech would be paid 10% of their hourly rate for just carrying the beeper. If they were working they would get time and a half for the actual hours worked and also paid the on call as well. If called in on a holiday they recieved premium pay which is 2X their hourly wage. The average on call for 34 hours was $85 which I think was pretty fair and better than most places I'd seen given that when you take on call your weekends are pretty limited as to what you can do. Good luck! On 1/4/08, Carrie Disbrow wrote: > We think our hourly pay to carry the beeper when on call is too low and we have talked to management. We supply coverage on weekends and holidays and we rotate the beeper weekly. > Our employer said if we took a survey and the results revealed our on-call pay is below average we might get an increase. > Would anyone like to participate in our survey? > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From katherine_dubrall <@t> yahoo.com Sat Jan 5 12:44:02 2008 From: katherine_dubrall <@t> yahoo.com (kay dubrall) Date: Sat Jan 5 12:44:32 2008 Subject: [Histonet] on call coverage In-Reply-To: <4f016b690801050928ke0a74acncb4a9f6744cca8e6@mail.gmail.com> Message-ID: <851859.57533.qm@web54203.mail.re2.yahoo.com> i've worked for two hospitals where call was required. in the first, we were paid $2.00 an hour for the entire weekend, day and night. if you were ever actually called in, you clocked in and were paid your hourly wage, plus they compensated with trip pay. where i'm currently working, you carry a cell phone and are on call four hours each evening and from 7 am till 10 pm each weekend day. we are paid our hourly rate for each four hours of call. if you are called in, you clock in and are paid your hourly rate for the time you actually work. there is no trip pay. good luck! k. what's kay is kay - j3 --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From Maxim_71 <@t> mail.ru Sat Jan 5 13:21:22 2008 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Sat Jan 5 13:23:34 2008 Subject: [Histonet] histochemistry an extinct art? Message-ID: <606658690.20080105222122@mail.ru> Gudrun: I also work in clinical histology lab. We do H&E and SS (up to 20 methods). Art of histochemistry will be always. Today it is for cost considerations vs IHC. When modern science moves upward, routine practice still uses many old methods with big success. And for comparison purposes any still use old classical methods. IHC and HC not will never disturbes to each other as airplanes, autos and pedestrians and will helps each other. Maxim Peshkov Taganrog, Russia. From gu.lang <@t> gmx.at Sat Jan 5 15:25:03 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Jan 5 15:25:28 2008 Subject: AW: [Histonet] histochemistry an extinct art? In-Reply-To: <606658690.20080105222122@mail.ru> Message-ID: <000c01c84fe1$701ffe80$eeeea8c0@dielangs.at> I also think, that the old staining methods have their right to exist and are an important part of modern histology. What I referred to in my former question were techniques like the demonstration and identification of proteins, aminoacids, enzymes, pigments. For example, I have just Lillie's book (1953) in front of me. I find protocols for the demonstration of arginin, tryptophan, urea, keratin, keratohyalin, plasmalogen. Or in Pearses book (1968) ninhydrin, DNA, RNA, ascorbic acid and so on. There are special fixations or cryotechniques recommended. And sometimes a translater would be helpfull. Not only from English to German, but also from trivialnames of reagenses. Are these methods still in use anywhere? Or survived only those, that can be done on FFPE-tissue? Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: Maxim Peshkov [mailto:Maxim_71@mail.ru] Gesendet: Samstag, 05. J?nner 2008 20:21 An: gu.lang@gmx.at Cc: histonet@lists.utsouthwestern.edu Betreff: Re: [Histonet] histochemistry an extinct art? Gudrun: I also work in clinical histology lab. We do H&E and SS (up to 20 methods). Art of histochemistry will be always. Today it is for cost considerations vs IHC. When modern science moves upward, routine practice still uses many old methods with big success. And for comparison purposes any still use old classical methods. IHC and HC not will never disturbes to each other as airplanes, autos and pedestrians and will helps each other. Maxim Peshkov Taganrog, Russia. From Maxim_71 <@t> mail.ru Sun Jan 6 05:49:32 2008 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Sun Jan 6 06:04:05 2008 Subject: AW: [Histonet] histochemistry an extinct art? In-Reply-To: <000c01c84fe1$701ffe80$eeeea8c0@dielangs.at> References: <606658690.20080105222122@mail.ru> <000c01c84fe1$701ffe80$eeeea8c0@dielangs.at> Message-ID: <1309250293.20080106144932@mail.ru> We also have Lillie, Pearse and Romeis books in russain traslations. Most techniqiues we found here. I learned english, because I need to know how work other people around the world and Histonet is one of very valuable resources. We work with FFPE and FS sections, because our department to do daily diagnostic work, which not require most complex methods. But I know, that in others lab, where do scientific work, use enzymatic and other techniquies if they need. Maxim Peshkov Taganrog, Russia. From JWEEMS <@t> sjha.org Sun Jan 6 07:31:19 2008 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Sun Jan 6 07:31:53 2008 Subject: AW: [Histonet] histochemistry an extinct art? References: <606658690.20080105222122@mail.ru><000c01c84fe1$701ffe80$eeeea8c0@dielangs.at> <1309250293.20080106144932@mail.ru> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3204726B4F@sjhaexc02.sjha.org> Freida Carson's book is one of the best for self help. If you don't have it you might want to check it out at Amazon.com - there is a text and a workbook. If you have students, this is really good resource. I wish that I could say I learned Russian so that I could communicate with you! But that goodness you learned English! I am so grateful to Herb and Linda for providing us the tool to share our questions and answers around the world. Thanks again Herb and Linda. Cheers, Maxim. j:>) Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Maxim Peshkov Sent: Sun 1/6/2008 6:49 AM To: Gudrun Lang Cc: histonet@lists.utsouthwestern.edu Subject: Re: AW: [Histonet] histochemistry an extinct art? We also have Lillie, Pearse and Romeis books in russain traslations. Most techniqiues we found here. I learned english, because I need to know how work other people around the world and Histonet is one of very valuable resources. We work with FFPE and FS sections, because our department to do daily diagnostic work, which not require most complex methods. But I know, that in others lab, where do scientific work, use enzymatic and other techniquies if they need. Maxim Peshkov Taganrog, Russia. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Malcolm.McCallum <@t> tamut.edu Sun Jan 6 21:08:49 2008 From: Malcolm.McCallum <@t> tamut.edu (Malcolm McCallum) Date: Sun Jan 6 21:10:12 2008 Subject: [Histonet] online histotechnology programs? References: Message-ID: just a curious question, are there any online histotechnology programs and are they good enough to get people certified?? M Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-223-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html VISIT HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org A New Journal Published in Partnership with Partners in Amphibian and Reptile Conservation and the World Congress of Herpetology. Fall Teaching Schedule & Office Hours: Mammalogy: MW 2-4 pm Cell Biology: TR 10-11:40 am Ecology: W 6-10 pm Offic Hours: MW 1-2, TR 11:40-12:40 "We live in a time when lemonade is made with artificial flavoring, and furnisher polish is made with fresh lemons." -Alfred E. Neuman -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Godfrey Guerzon Sent: Fri 1/4/2008 3:07 PM To: histonet@lists.utsouthwestern.edu; Shanu Mehta Subject: Re: [Histonet] Course in Histology There is a web course in Histotechnology at Harford Community College in Maryland. You may want to check it out. The Director of the program is Mr. Floyd Grimm but I believe he is retiring. His phone number is (410) 836-4372. His e-mail address fgrimm@harford.edu. You may also check the college website. Godfrey >>> "Shanu Mehta" 1/4/2008 2:34 PM >>> Hello Histonetters, Is there a histology course I can take anywhere in the Boston area? I did not take any formal training/education in Histology but have been doing the same for almost 2 years now. It would be great if I could get some formal knowledge in Histology to help me understand the basic problems I face sometimes during my routine work. Thanks a lot for any help with this. Shanu ------------ Shanu Mehta Magen Biosciences 100 Beaver St.Suite 101 Waltham, MA 02453 Direct Dial: 781-314-2918 Fax: 781-314-2999 Main Line: 781-314-2900 Email: smehta@magenbiosciences.com http://www.magenbiosciences.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------------------------------- THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE ADDRESSEE LISTED ABOVE. This record has been disclosed in accordance with Subtitle 3 of Title 4 of the Health-General Article of the Annotated Code of Maryland. Further disclosure of medical information contained herein is prohibited. If you are neither the intended recipient nor the individual responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure of patient information is strictly prohibited. If you have received this email in error, immediately notify us by telephone or return email. ----------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Andrew.Prior <@t> Smith-Nephew.com Mon Jan 7 03:50:30 2008 From: Andrew.Prior <@t> Smith-Nephew.com (Prior, Andrew) Date: Mon Jan 7 03:50:52 2008 Subject: [Histonet] Exposure levels Message-ID: <7028DD15E14FDC4DB1F3C5AF8735AF3704CBB8BE@EHS021.wound.san> Following a chat with our Health & Safety Officer, I'm looking for suggestions on how to monitor air levels of the following chemicals: Xylene Chloroform Formalin We have a well vented lab and follow all the usual precautions but would like to check that we are not poisoning ourselves too much. How do you measure exposure in your labs? Ideally I'm looking for a badge that a person could wear to measure individual exposure, but I don't know if they exist. Our Safety Officer has looked in to this, but has been unable to come up with a definite answer so I thought I would turn to the experts. Thanks Andrew. Andrew Prior Histologist Smith &Nephew Research Centre York Science Park Heslington York YO10 5DF UK Andrew.Prior@smith-nephew.com 01904 824022 Confidentiality. This electronic transmission is strictly confidential to Smith & Nephew and intended solely for the addressee. It may contain information which is covered by legal, professional or other privilege. If you are not the intended addressee, or someone authorised by the intended addressee to receive transmissions on behalf of the addressee, you must not retain, disclose in any form, copy or take any action in reliance on this transmission. If you have received this transmission in error, please notify the sender as soon as possible and destroy this message. Smith & Nephew UK Limited Registered in England and Wales No.4421171 with registered office at 15 Adam Street, London WC2N 6LA From jnocito <@t> satx.rr.com Mon Jan 7 05:49:20 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Jan 7 05:49:33 2008 Subject: [Histonet] Exposure levels References: <7028DD15E14FDC4DB1F3C5AF8735AF3704CBB8BE@EHS021.wound.san> Message-ID: <001b01c85123$5782bc30$0302a8c0@yourxhtr8hvc4p> Andrew, please try this company. I have been using them for 10 years. They have badges for most chemicals. I have found them to be reliable and really helpful. Good luck Joe www.enviroguard.com/services2.html ----- Original Message ----- From: "Prior, Andrew" To: Sent: Monday, January 07, 2008 3:50 AM Subject: [Histonet] Exposure levels Following a chat with our Health & Safety Officer, I'm looking for suggestions on how to monitor air levels of the following chemicals: Xylene Chloroform Formalin We have a well vented lab and follow all the usual precautions but would like to check that we are not poisoning ourselves too much. How do you measure exposure in your labs? Ideally I'm looking for a badge that a person could wear to measure individual exposure, but I don't know if they exist. Our Safety Officer has looked in to this, but has been unable to come up with a definite answer so I thought I would turn to the experts. Thanks Andrew. Andrew Prior Histologist Smith &Nephew Research Centre York Science Park Heslington York YO10 5DF UK Andrew.Prior@smith-nephew.com 01904 824022 Confidentiality. This electronic transmission is strictly confidential to Smith & Nephew and intended solely for the addressee. It may contain information which is covered by legal, professional or other privilege. If you are not the intended addressee, or someone authorised by the intended addressee to receive transmissions on behalf of the addressee, you must not retain, disclose in any form, copy or take any action in reliance on this transmission. If you have received this transmission in error, please notify the sender as soon as possible and destroy this message. Smith & Nephew UK Limited Registered in England and Wales No.4421171 with registered office at 15 Adam Street, London WC2N 6LA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AReam <@t> challiance.org Mon Jan 7 06:28:07 2008 From: AReam <@t> challiance.org (Ream, Arthur) Date: Mon Jan 7 06:28:33 2008 Subject: [Histonet] Cassette labeler interfaced Meditech Message-ID: Good Morning, Does anyone have or know of a Cassette labeler that is used with Meditech Client Server. Regards, Arthur F. Ream III Applications Analyst Cambridge Health Alliance #65 Beacon Street, Somerville MA 02143 617-665-2353 - Office 617-665-2097 - Fax aream@challiance.org From micro <@t> superlink.net Mon Jan 7 06:38:47 2008 From: micro <@t> superlink.net (Markus F. Meyenhofer) Date: Mon Jan 7 06:42:38 2008 Subject: [Histonet] Exposure levels References: <7028DD15E14FDC4DB1F3C5AF8735AF3704CBB8BE@EHS021.wound.san> Message-ID: <1d1701c8512a$ac019400$39893cd1@DJ4VDH31> Some "Health & Safety Officer"!!! ----- Original Message ----- From: "Prior, Andrew" To: Sent: Monday, January 07, 2008 4:50 AM Subject: [Histonet] Exposure levels Following a chat with our Health & Safety Officer, I'm looking for suggestions on how to monitor air levels of the following chemicals: Xylene Chloroform Formalin We have a well vented lab and follow all the usual precautions but would like to check that we are not poisoning ourselves too much. How do you measure exposure in your labs? Ideally I'm looking for a badge that a person could wear to measure individual exposure, but I don't know if they exist. Our Safety Officer has looked in to this, but has been unable to come up with a definite answer so I thought I would turn to the experts. Thanks Andrew. Andrew Prior Histologist Smith &Nephew Research Centre York Science Park Heslington York YO10 5DF UK Andrew.Prior@smith-nephew.com 01904 824022 Confidentiality. This electronic transmission is strictly confidential to Smith & Nephew and intended solely for the addressee. It may contain information which is covered by legal, professional or other privilege. If you are not the intended addressee, or someone authorised by the intended addressee to receive transmissions on behalf of the addressee, you must not retain, disclose in any form, copy or take any action in reliance on this transmission. If you have received this transmission in error, please notify the sender as soon as possible and destroy this message. Smith & Nephew UK Limited Registered in England and Wales No.4421171 with registered office at 15 Adam Street, London WC2N 6LA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Andrew.Prior <@t> Smith-Nephew.com Mon Jan 7 07:01:32 2008 From: Andrew.Prior <@t> Smith-Nephew.com (Prior, Andrew) Date: Mon Jan 7 07:01:58 2008 Subject: [Histonet] Exposure levels In-Reply-To: <1d1701c8512a$ac019400$39893cd1@DJ4VDH31> References: <7028DD15E14FDC4DB1F3C5AF8735AF3704CBB8BE@EHS021.wound.san> <1d1701c8512a$ac019400$39893cd1@DJ4VDH31> Message-ID: <7028DD15E14FDC4DB1F3C5AF8735AF3704CBBCAF@EHS021.wound.san> Actually, he's a good H&S officer! (just a bit inexperienced in this field). Thankfully he has the brains to know when he doesn't have the answers and isn't afraid to ask for help (through me) from those more experienced to make sure that we are kept safe. Andrew -----Original Message----- From: Markus F. Meyenhofer [mailto:micro@superlink.net] Sent: 07 January 2008 12:39 To: Prior, Andrew; histonet@lists.utsouthwestern.edu Subject: *****[ Possible Spam ]**** Re: [Histonet] Exposure levels Importance: Low Some "Health & Safety Officer"!!! ----- Original Message ----- From: "Prior, Andrew" To: Sent: Monday, January 07, 2008 4:50 AM Subject: [Histonet] Exposure levels Following a chat with our Health & Safety Officer, I'm looking for suggestions on how to monitor air levels of the following chemicals: Xylene Chloroform Formalin We have a well vented lab and follow all the usual precautions but would like to check that we are not poisoning ourselves too much. How do you measure exposure in your labs? Ideally I'm looking for a badge that a person could wear to measure individual exposure, but I don't know if they exist. Our Safety Officer has looked in to this, but has been unable to come up with a definite answer so I thought I would turn to the experts. Thanks Andrew. Andrew Prior Histologist Smith &Nephew Research Centre York Science Park Heslington York YO10 5DF UK Andrew.Prior@smith-nephew.com 01904 824022 Confidentiality. This electronic transmission is strictly confidential to Smith & Nephew and intended solely for the addressee. It may contain information which is covered by legal, professional or other privilege. If you are not the intended addressee, or someone authorised by the intended addressee to receive transmissions on behalf of the addressee, you must not retain, disclose in any form, copy or take any action in reliance on this transmission. If you have received this transmission in error, please notify the sender as soon as possible and destroy this message. Smith & Nephew UK Limited Registered in England and Wales No.4421171 with registered office at 15 Adam Street, London WC2N 6LA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality. This electronic transmission is strictly confidential to Smith & Nephew and intended solely for the addressee. It may contain information which is covered by legal, professional or other privilege. If you are not the intended addressee, or someone authorised by the intended addressee to receive transmissions on behalf of the addressee, you must not retain, disclose in any form, copy or take any action in reliance on this transmission. If you have received this transmission in error, please notify the sender as soon as possible and destroy this message. Smith & Nephew UK Limited Registered in England and Wales No.4421171 with registered office at 15 Adam Street, London WC2N 6LA From mpence <@t> grhs.net Mon Jan 7 08:20:56 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Jan 7 08:21:20 2008 Subject: [Histonet] Exposure levels In-Reply-To: <7028DD15E14FDC4DB1F3C5AF8735AF3704CBB8BE@EHS021.wound.san> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C84D@IS-E2K3.grhs.net> There are many vendors that offer a badge for monitoring the chemicals you mention. Here is one link that might get you started. http://www.labsafety.com/search/badges/2304/ I am not promoting a vendor here just am example of what to look for. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Prior, Andrew Sent: Monday, January 07, 2008 3:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Exposure levels Following a chat with our Health & Safety Officer, I'm looking for suggestions on how to monitor air levels of the following chemicals: Xylene Chloroform Formalin We have a well vented lab and follow all the usual precautions but would like to check that we are not poisoning ourselves too much. How do you measure exposure in your labs? Ideally I'm looking for a badge that a person could wear to measure individual exposure, but I don't know if they exist. Our Safety Officer has looked in to this, but has been unable to come up with a definite answer so I thought I would turn to the experts. Thanks Andrew. Andrew Prior Histologist Smith &Nephew Research Centre York Science Park Heslington York YO10 5DF UK Andrew.Prior@smith-nephew.com 01904 824022 Confidentiality. This electronic transmission is strictly confidential to Smith & Nephew and intended solely for the addressee. It may contain information which is covered by legal, professional or other privilege. If you are not the intended addressee, or someone authorised by the intended addressee to receive transmissions on behalf of the addressee, you must not retain, disclose in any form, copy or take any action in reliance on this transmission. If you have received this transmission in error, please notify the sender as soon as possible and destroy this message. Smith & Nephew UK Limited Registered in England and Wales No.4421171 with registered office at 15 Adam Street, London WC2N 6LA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From karenadams <@t> comcast.net Mon Jan 7 08:40:36 2008 From: karenadams <@t> comcast.net (karenadams@comcast.net) Date: Mon Jan 7 08:41:00 2008 Subject: [Histonet] speaking of monitoring badges Message-ID: <010720081440.4893.478239E4000C72780000131D22070206539C030E0B0E020A9D0E05@comcast.net> ....does anyone know of a badge that can be worn in case of formalin spill, that would display the ppm exposure at that time? This would be in the case of a moderate spill given respirators are used. -- Karen Adams Supervisor Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 From JGREWE <@t> OhioHealth.com Mon Jan 7 08:51:58 2008 From: JGREWE <@t> OhioHealth.com (JGREWE@OhioHealth.com) Date: Mon Jan 7 08:52:25 2008 Subject: [Histonet] Peloris Feedback Message-ID: Can anyone provide me with both good and bad feedback for the Peloris Tissue Processor? Thanks Jacquelyn L. Grewe BS, HT (ASCP) Laboratory Supervisor, Anatomic Pathology Riverside Methodist Hospital 614-566-4562 or 614-566-5679 _______________________________________________ FORTUNE "100 Best Companies to Work for 2007 " From Gguerzon <@t> lifebridgehealth.org Mon Jan 7 09:06:38 2008 From: Gguerzon <@t> lifebridgehealth.org (Godfrey Guerzon) Date: Mon Jan 7 09:08:03 2008 Subject: [Histonet] Re: online histotechnology programs? Message-ID: Malcolm, There is a web course in Histotechnology at Harford Community College in Maryland. You may want to check it out. The Director of the program is Mr. Floyd Grimm but I believe he is retiring. His phone number is (410) 836-4372. His e-mail address fgrimm@harford.edu. You may also check the college website. This program provides the theoretical aspects on the web. The student, if working in a Histology laboratory can be on a certificate program, if not working in a Histology Lab it will be an associate degree. The college will work with an accrdited Histology laboratory in the area where the student reside for clinical (practical) rotation. The college also provide the student with a student guide of what needs to be covered on the clinical rotation that has to be signed off as completed by the Histology Manager (or designee). I have Histotechs in my Lab several students from Harford Community College that are doing very well and are already certified HT(ASCP). Our Lab is one of the Hospital in Maryland that provides clinical rotation for these students. Godfrey >>> "Malcolm McCallum" 1/6/2008 10:08 PM >>> just a curious question, are there any online histotechnology programs and are they good enough to get people certified?? M Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-223-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html VISIT HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org A New Journal Published in Partnership with Partners in Amphibian and Reptile Conservation and the World Congress of Herpetology. Fall Teaching Schedule & Office Hours: Mammalogy: MW 2-4 pm Cell Biology: TR 10-11:40 am Ecology: W 6-10 pm Offic Hours: MW 1-2, TR 11:40-12:40 "We live in a time when lemonade is made with artificial flavoring, and furnisher polish is made with fresh lemons." -Alfred E. Neuman -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Godfrey Guerzon Sent: Fri 1/4/2008 3:07 PM To: histonet@lists.utsouthwestern.edu; Shanu Mehta Subject: Re: [Histonet] Course in Histology There is a web course in Histotechnology at Harford Community College in Maryland. You may want to check it out. The Director of the program is Mr. Floyd Grimm but I believe he is retiring. His phone number is (410) 836-4372. His e-mail address fgrimm@harford.edu. You may also check the college website. Godfrey >>> "Shanu Mehta" 1/4/2008 2:34 PM >>> Hello Histonetters, Is there a histology course I can take anywhere in the Boston area? I did not take any formal training/education in Histology but have been doing the same for almost 2 years now. It would be great if I could get some formal knowledge in Histology to help me understand the basic problems I face sometimes during my routine work. Thanks a lot for any help with this. Shanu ------------ Shanu Mehta Magen Biosciences 100 Beaver St.Suite 101 Waltham, MA 02453 Direct Dial: 781-314-2918 Fax: 781-314-2999 Main Line: 781-314-2900 Email: smehta@magenbiosciences.com http://www.magenbiosciences.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------------------------------- THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE ADDRESSEE LISTED ABOVE. This record has been disclosed in accordance with Subtitle 3 of Title 4 of the Health-General Article of the Annotated Code of Maryland. Further disclosure of medical information contained herein is prohibited. If you are neither the intended recipient nor the individual responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure of patient information is strictly prohibited. If you have received this email in error, immediately notify us by telephone or return email. ----------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------------------------------- THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE ADDRESSEE LISTED ABOVE. This record has been disclosed in accordance with Subtitle 3 of Title 4 of the Health-General Article of the Annotated Code of Maryland. Further disclosure of medical information contained herein is prohibited. If you are neither the intended recipient nor the individual responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure of patient information is strictly prohibited. If you have received this email in error, immediately notify us by telephone or return email. ----------------------------------------------------------------- From Sheri.Meilus <@t> va.gov Mon Jan 7 09:08:23 2008 From: Sheri.Meilus <@t> va.gov (Meilus, Sheri D.) Date: Mon Jan 7 09:08:45 2008 Subject: [Histonet] RE: CJD procedures In-Reply-To: References: Message-ID: I have found the National Prion Disease Pathology Surveillance Center to be very helpful. Their web address is www.cjdsurveillance.com S Sheri Meilus, HT(ASCP)QIHC Anatomic Pathology Supervisor Bay Pines VAMC Bay Pines, FL 33744 727-398-6661 4596 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Saturday, January 05, 2008 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 50, Issue 6 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Histonet Digest, Vol 50, Issue 5 (Cook, Sharon ) 2. Re: RE: HT and HTL "Salary Survey" posting (Kim Merriam) 3. PEEK and PLLA/PGA (Nancy W. Troiano) 4. Ki-67 (Kalleberg, Kristopher) 5. RE: Pressure cooker question (Jo Dee Fish) 6. CJD Histology Procedures (Dawson, Glen) 7. Re: Re: Lendrum stains (Bryan Llewellyn) 8. histochemistry an extinct art? (Gudrun Lang) 9. RE: Re: Lendrum stains (Houston, Ronald) 10. Course in Histology (Shanu Mehta) 11. Sausage blocks (Webb, Dorothy L) 12. Surfactant A protein (Beth Delescavage) 13. Re: CJD Histology Procedures (Amy Porter) 14. Re: Course in Histology (Godfrey Guerzon) 15. jackson immuno backorder (Emily Sours) 16. Re: histochemistry an extinct art? (Bryan Llewellyn) 17. Re: histochemistry an extinct art? (Rene J Buesa) 18. on call coverage (Carrie Disbrow) 19. RE: on call coverage (Weems, Joyce) 20. Re: immunocal (Maxim Peshkov) 21. RE: immunocal (Weems, Joyce) 22. Re: on call coverage (Victoria Baker) ---------------------------------------------------------------------- Message: 1 Date: Fri, 4 Jan 2008 13:04:37 -0500 From: "Cook, Sharon " Subject: [Histonet] RE: Histonet Digest, Vol 50, Issue 5 To: Message-ID: <7A541592F9A53A4E86E7A3D5C4C7F68C01D7962B@msxc01.osumc.edu> Content-Type: text/plain; charset="us-ascii" I've unsubscribed 2 times and I continue to receive these emails. Please unsubscribe for me. Thanks, Sharon S. Cook, MT(ASCP) SBB Operations Director, Anatomic Pathology The Ohio State University E407 Doan Hall 410 West 10th Avenue Columbus, OH 43210 sharon.cook@osumc.edu Phone: 614 / 293-8418 Fax: 614 / 293-2779 Pager: 614 / 346-0746 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, January 04, 2008 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 50, Issue 5 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Lendrum stains (Robert Richmond) ---------------------------------------------------------------------- Message: 1 Date: Fri, 4 Jan 2008 12:50:03 -0500 From: "Robert Richmond" Subject: [Histonet] Re: Lendrum stains To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Sharon E. Willman (where?) asks for information about "Lendrun" and Fraser stains, she doesn't say for what. A.C. Lendrum, I was told back around 1970, was one of the last histologists who understood textile dyes. Apparently there were a lot of small factories making obscure dyes in Scotland back then, and he knew all of these single malt dye-makers and collected numerous samples. His eclectic habits make it difficult to identify the dyes he used, let alone obtain and use them. That and the invention of immunohistochemistry probably consign most of his work to oblivion. Lendrum's "Obadiah" stain (he was fond of such fanciful names) for fibrin was in use in a research lab at Cornell Medical Center on east 68th street in New York City when I was there briefly in 1968. Stainsfile gives the following reference: Lendrum, A. C., et. al. (1962) "Studies on the character and staining of fibrin." Journal of Clinical Pathology, v. 15, p. 401. [this is a British journal, not the AJCP]. Rotsa ruck finding naphthalene blue black CS, Chicago red, or polar brilliant red BN. John Kiernan - Dick Dapson - Mike Titford - other geezers on this list - do you know anything more about A.C. Lendrum? He must have been an interesting guy! Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 50, Issue 5 *************************************** ------------------------------ Message: 2 Date: Fri, 4 Jan 2008 10:21:23 -0800 (PST) From: Kim Merriam Subject: Re: [Histonet] RE: HT and HTL "Salary Survey" posting To: relia1@earthlink.net, Histonet Message-ID: <564776.10450.qm@web50306.mail.re2.yahoo.com> Content-Type: text/plain; charset=us-ascii Thanks for the tip Pam. I have actually had #3 happen to me (about 10 years ago or so): I had been at my new job for about 3 months and I got a call from someone in HR telling me that she had just received my resume from a recruiter. I had to explain to her that, obviously, I had been looking for a position prior to my arrival at that company so it must have been a left-over, but it was nevertheless an embarrassing moment for me (she was less than understanding - "human" relations was not her strong point; not so good for someone working in HR). I called the recruiter and gave him a piece of my mind!! He should not have sent my resume to anyone without my permission. Kim Merriam, MA, HT(ASCP) Cambridge, MA ----- Original Message ---- From: Pam Barker To: Histonet Sent: Thursday, January 3, 2008 4:43:08 PM Subject: [Histonet] RE: HT and HTL "Salary Survey" posting Hi Histonetters! I hope everybody had a wonderful holiday season. I just read the posting from Robert Garhart a recruiter at System One Search. I just wanted to comment to those of you who might not be aware that by releasing this information you are actually releasing basic pre interview information. I am not saying don't do it I am just saying be aware of how the information could potentially be used. (i.e. Your information could be released to an employer for a potential job opening without your permission). By releasing this information you could be giving up your confidentiality. Having been a professional recruiter for over 25 years I have seen these types of requests before and more importantly the havoc it can wreak for an individual. Here are several scenarios I have seen: Each of these scenarios starts this way: The recruiter takes the information you have supplied for this "survey" and gives it to a client (a fishing expedition of sorts), without your knowledge or permission, then once there is interest from the client they contact you, regardless of whether or not you are looking for a job. Here are the potential outcomes: 1. You send your resume to a client and are not considered for the position because you have already been submitted by a recruiter without your knowledge or permission and the client is not paying fees at that time. 2. You are working with a recruiter who you have consented to have represent you at a client and that recruiter who you trust to represent you has accurately given you all the information on the position before submitting your resume and has done all of the footwork with the client is excluded from your placement because you have been submitted by another recruiter without your knowledge or permission. In this scenario you could also be cut from consideration because employers don't like to get caught up in conflicts between recruiters. Not to mention it makes you the candidate, look unprofessional by not realizing that someone you have not given permission to is representing you. 3. Your resume is sent to your current employer or an affiliate facility without your permission or knowledge - OUCH!!. 4. By giving this information you could be in violation of your company's own confidentialty policy. This information could also be sold for SPAM or used to build a candidate database. And as most of you know the information he is looking for is actually available in a confidential format free of charge at both the NSH and ASCP websites and at the website Salary.com I am not saying don't respond I am merely saying be aware of what could potentially result from responding. In this day and age it is very important to have control of your personal information, who you give it out to and how it is used. Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.myspace.com/pamatrelia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ ------------------------------ Message: 3 Date: Fri, 04 Jan 2008 13:22:59 -0500 From: "Nancy W. Troiano" Subject: [Histonet] PEEK and PLLA/PGA To: histonet@lists.utsouthwestern.edu Message-ID: <5.2.1.1.2.20080104132124.024477f0@email.med.yale.edu> Content-Type: text/plain; format=flowed; charset=us-ascii Hi Joanne - we have cut blood vessels containing PLLA and PGA implants and use glycolmethacrylate since the PLLA and PGA can tear through paraffin. You may want to try GMA - we use the Technovit 7100 kit. ------------------------------ Message: 4 Date: Fri, 4 Jan 2008 13:25:58 -0500 From: "Kalleberg, Kristopher" Subject: [Histonet] Ki-67 To: Message-ID: <0E6BC087F70F9C47ACFF2C203D6E329C0376DE99@NTRSEVS30002.s3.ms.unilever.com> Content-Type: text/plain; charset="us-ascii" Hello All, I know most people have worked with the biomarker Ki-67. The question that has come about is why a few nuclei are staining in suprabasal layers of skin and not just specifically in the basal layer where they should be specifically assigned to. I am investigating both photoprotected sites of the arm along with solar lentigines (sun spots/freckles) of the arm. If anyone can help me answer this, I would greatly appreciate it. Thank you. Kris Kalleberg ------------------------------ Message: 5 Date: Fri, 4 Jan 2008 10:34:10 -0800 From: "Jo Dee Fish" Subject: RE: [Histonet] Pressure cooker question To: Message-ID: <000001c84f00$65c7db10$2e0d010a@JFISH> Content-Type: text/plain; charset="us-ascii" Hello everyone, I just want to thank all of those who responded to my pressure cooker question. I have decided to order a "3-in-1" cooker from Sears. It is able to be more flexible, it can either steam or pressure cook, as well as slow cook (which we won't be doing unless we make BBQ meatballs to go with our beer on Fridays!), so we can try different temperatures and times to optimize our protocol. I understand that the "scientific" pressure cookers are probably more accurate as far as temperature is concerned, but the price is prohibitive which is why I decided to buy the less expensive household version. Thanks for all of your help and advice, Jo Dee ~~Jo Dee Fish~~ Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 -----Original Message----- From: Tom McNemar [mailto:TMcNemar@lmhealth.org] Sent: Friday, January 04, 2008 6:28 AM To: Bernice Frederick; jfish@gladstone.ucsf.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Pressure cooker question Interesting. I used the Biocare decloaker for 5 minutes and charged slides. My tissues came off at least 50% of the time. Do you let your slides dry overnight before running them? Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: Bernice Frederick [mailto:b-frederick@northwestern.edu] Sent: Friday, January 04, 2008 8:54 AM To: Tom McNemar; jfish@gladstone.ucsf.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Pressure cooker question We use the Biocare decloaker for antigen retrieval. It is a pressure cooker and specifically programmed. Our sections stay on. We like to cook the slides overnight. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Friday, January 04, 2008 6:43 AM To: jfish@gladstone.ucsf.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Pressure cooker question I used to use a pressure cooker but found it to be very harsh. I had a lot of trouble getting the sections to stay on the slide. For the last several years I'v use a $40 Black and Decker rice steamer. Slower than the pressure cooker but it always works and no problems with the sections. You might also look into microwave retrieval. I tried it a few times and it worked fine. I steam them for 20 minutes with a 20 minute cooling afterward. That gives me plenty of time to make up my buffer and set up my run. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jo Dee Fish Sent: Thursday, January 03, 2008 3:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pressure cooker question We are searching for a cheap but reliable way to do antigen retrieval. Is anyone out there using an electric pressure cooker? And, are the ordinary household appliances working for you? Thanks in advance for your help. Jo Dee ~~Jo Dee Fish~~ Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 4 Jan 2008 12:41:38 -0600 From: "Dawson, Glen" Subject: [Histonet] CJD Histology Procedures To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" All, Could someone point me in the right direction for the most up-to-date CJD Procedures for the Histolab. Ours may be out of date and I need something to compare them too. Thanx In Advance, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI ------------------------------ Message: 7 Date: Fri, 04 Jan 2008 10:58:28 -0800 From: Bryan Llewellyn Subject: Re: [Histonet] Re: Lendrum stains To: Histonet Message-ID: <000801c84f03$cb9ba310$05024246@yourlk4rlmsu> Content-Type: text/plain; format=flowed; charset=iso-8859-1; reply-type=original I trained in the UK during the early 1960s and went to Bromley Technical College every Friday night, a real pain since it was on the edge of South London and I lived on the edge of North London. The instructor was Wallington, of the text book, and he was regularly in contact with Lendrum. Even in those days (63 and 64) Lendrum was an exception among "dye tinkerers". Wallington told us that producing new methods for fibrin was pretty much a hobby for Lendrum. The one noted as the long method on StainsFile is actually from a variation given to Wallington by Lendrum earlier in that week of that Friday's lecture. I used to use all those methods when I was in Winnipeg during the 1970s, mostly on rejecting kidney transplants. Even then immunifluorescence was replacing staining methods for fibrin, certainly on renal biopsies. I always found the Obadiah an unsatisfactory stain, the Masson 44/41 too. The colours just didn't look right to me. The picro-mallory is, however, one of the stars in trichrome staining if done on properly prepared material. I always found it absolutely beautiful to look at, but then, I am noted as being a bit strange about things like that. I have substituted amido black 10B (naphthol blue black, CI # 20470) for naphthalene blue black CS, and it worked fine. They are structurally very similar. Bryan Llewellyn ----- Original Message ----- From: "Robert Richmond" To: Sent: Friday, January 04, 2008 9:50 AM Subject: [Histonet] Re: Lendrum stains > Sharon E. Willman (where?) asks for information about "Lendrun" and > Fraser stains, she doesn't say for what. > > A.C. Lendrum, I was told back around 1970, was one of the last > histologists who understood textile dyes. Apparently there were a lot > of small factories making obscure dyes in Scotland back then, and he > knew all of these single malt dye-makers and collected numerous > samples. His eclectic habits make it difficult to identify the dyes he > used, let alone obtain and use them. That and the invention of > immunohistochemistry probably consign most of his work to oblivion. > > Lendrum's "Obadiah" stain (he was fond of such fanciful names) for > fibrin was in use in a research lab at Cornell Medical Center on east > 68th street in New York City when I was there briefly in 1968. > Stainsfile gives the following reference: Lendrum, A. C., et. al. > (1962) "Studies on the character and staining of fibrin." Journal of > Clinical Pathology, v. 15, p. 401. [this is a British journal, not the > AJCP]. Rotsa ruck finding naphthalene blue black CS, Chicago red, or > polar brilliant red BN. > > John Kiernan - Dick Dapson - Mike Titford - other geezers on this list > - do you know anything more about A.C. Lendrum? He must have been an > interesting guy! > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Fri, 4 Jan 2008 20:26:11 +0100 From: "Gudrun Lang" Subject: [Histonet] histochemistry an extinct art? To: Message-ID: <000501c84f07$aad39ee0$eeeea8c0@dielangs.at> Content-Type: text/plain; charset="us-ascii" Hi, I have a question for those, that are experienced with the old and the new techniques in histotechnology. I looked through some antiquariat-books, that deal a lot with histochemical demonstration of many tissue-compounds and enzymes. These techniques sound all very strange to me and it seems like a forgotten art. I work in a clinical histolab. Are the histochemical, enzymhistochemical methods still in use in research-labs? Did immunhistochemistry replace them all together? Is histochemistry a part of modern cellbiology or just obsolete? Gudrun Lang ------------------------------ Message: 9 Date: Fri, 4 Jan 2008 14:25:56 -0500 From: "Houston, Ronald" Subject: RE: [Histonet] Re: Lendrum stains To: "Robert Richmond" , Message-ID: <979FF5962E234F45B06CF0DB7C1AABB2145D9FC8@chi2k3ms01.columbuschildrens.net> Content-Type: text/plain; charset="us-ascii" I fondly remember performing Lendrum's stains in a previous life back home in Scotland. The color contrasts of many of the techniques remains unsurpassed as far as tinctorial staining is concerned. Not so sure why none of the methods really took off in the States; perhaps you're right, Bob, it may have had to do with the fact that he got many of the dyes directly from the mills. Professor Alan C Lendrum was Professor of Pathology at both Glasgow and Dundee Universities (the latter until 1972 when I believe he retired). Perhaps John Bancroft, Peter Stoward and/or Richard Horobin would be able to comment more on Professor Lendrum, if they still subscribe to Histonet. It is certainly true that he was one of the last histopathologists who understood the properties of the dyes and the theory behind the techniques he introduced with his colleagues. His work was true Art in histology; alas a dieing trait (no pun intended). As far as I am concerned, his MSB (Martius Yellow, Crystal Scarlet, Soluble Blue) is still the best method for fibrin demonstration. Ronnie Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 Ronald.Houston@NationwideChildrens.org Columbus Children's Hospital is now Nationwide Children's Hospital www.NationwideChildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Friday, January 04, 2008 12:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Lendrum stains Sharon E. Willman (where?) asks for information about "Lendrun" and Fraser stains, she doesn't say for what. A.C. Lendrum, I was told back around 1970, was one of the last histologists who understood textile dyes. Apparently there were a lot of small factories making obscure dyes in Scotland back then, and he knew all of these single malt dye-makers and collected numerous samples. His eclectic habits make it difficult to identify the dyes he used, let alone obtain and use them. That and the invention of immunohistochemistry probably consign most of his work to oblivion. Lendrum's "Obadiah" stain (he was fond of such fanciful names) for fibrin was in use in a research lab at Cornell Medical Center on east 68th street in New York City when I was there briefly in 1968. Stainsfile gives the following reference: Lendrum, A. C., et. al. (1962) "Studies on the character and staining of fibrin." Journal of Clinical Pathology, v. 15, p. 401. [this is a British journal, not the AJCP]. Rotsa ruck finding naphthalene blue black CS, Chicago red, or polar brilliant red BN. John Kiernan - Dick Dapson - Mike Titford - other geezers on this list - do you know anything more about A.C. Lendrum? He must have been an interesting guy! Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ------------------------------ Message: 10 Date: Fri, 4 Jan 2008 14:34:25 -0500 From: "Shanu Mehta" Subject: [Histonet] Course in Histology To: Message-ID: <4C78CF3C61E6A640888226885709EFE9379DAB@server01.magen.local> Content-Type: text/plain; charset="US-ASCII" Hello Histonetters, Is there a histology course I can take anywhere in the Boston area? I did not take any formal training/education in Histology but have been doing the same for almost 2 years now. It would be great if I could get some formal knowledge in Histology to help me understand the basic problems I face sometimes during my routine work. Thanks a lot for any help with this. Shanu ------------ Shanu Mehta Magen Biosciences 100 Beaver St.Suite 101 Waltham, MA 02453 Direct Dial: 781-314-2918 Fax: 781-314-2999 Main Line: 781-314-2900 Email: smehta@magenbiosciences.com http://www.magenbiosciences.com ------------------------------ Message: 11 Date: Fri, 04 Jan 2008 13:46:30 -0600 From: "Webb, Dorothy L" Subject: [Histonet] Sausage blocks To: histonet@lists.utsouthwestern.edu Message-ID: <0E394B648E5284478A6CCB78E5AFDA2705635392@hpes1.HealthPartners.int> Content-Type: text/plain; charset="us-ascii" Does anyone use sausage blocks for their IHC controls? I have heard pros and cons to this control usage, the cons being mostly too time consuming to keep up. Would like some input as to what others are doing in this regard!! Thanks ahead of time!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. ------------------------------ Message: 12 Date: Fri, 4 Jan 2008 12:00:22 -0800 From: "Beth Delescavage" Subject: [Histonet] Surfactant A protein To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi All~ Thermo/Neomarkers/ Lab Vision has discontinued the Surfactant A clone PE10 antibody we have been using and are not offering any replacements. Are any of you using the same clone from a different vendor and do you like the staining? Have a nice weekend! ~Beth Beth Delescavage, BS, HTL (ASCP) QIHC CellNetix Laboratories Histotechnologist DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. ------------------------------ Message: 13 Date: Fri, 4 Jan 2008 15:47:41 -0500 From: "Amy Porter" Subject: Re: [Histonet] CJD Histology Procedures To: "Dawson, Glen" , Message-ID: <002001c84f13$0c9b33d0$8e7a0923@histolab> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original I would recommend the CDC website. Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 355-6475 ext 1480 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu ----- Original Message ----- From: "Dawson, Glen" To: Sent: Friday, January 04, 2008 1:41 PM Subject: [Histonet] CJD Histology Procedures All, Could someone point me in the right direction for the most up-to-date CJD Procedures for the Histolab. Ours may be out of date and I need something to compare them too. Thanx In Advance, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Fri, 04 Jan 2008 16:07:23 -0500 From: "Godfrey Guerzon" Subject: Re: [Histonet] Course in Histology To: , "Shanu Mehta" Message-ID: Content-Type: text/plain; charset=US-ASCII There is a web course in Histotechnology at Harford Community College in Maryland. You may want to check it out. The Director of the program is Mr. Floyd Grimm but I believe he is retiring. His phone number is (410) 836-4372. His e-mail address fgrimm@harford.edu. You may also check the college website. Godfrey >>> "Shanu Mehta" 1/4/2008 2:34 PM >>> Hello Histonetters, Is there a histology course I can take anywhere in the Boston area? I did not take any formal training/education in Histology but have been doing the same for almost 2 years now. It would be great if I could get some formal knowledge in Histology to help me understand the basic problems I face sometimes during my routine work. Thanks a lot for any help with this. Shanu ------------ Shanu Mehta Magen Biosciences 100 Beaver St.Suite 101 Waltham, MA 02453 Direct Dial: 781-314-2918 Fax: 781-314-2999 Main Line: 781-314-2900 Email: smehta@magenbiosciences.com http://www.magenbiosciences.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------------------------------- THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE ADDRESSEE LISTED ABOVE. This record has been disclosed in accordance with Subtitle 3 of Title 4 of the Health-General Article of the Annotated Code of Maryland. Further disclosure of medical information contained herein is prohibited. If you are neither the intended recipient nor the individual responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure of patient information is strictly prohibited. If you have received this email in error, immediately notify us by telephone or return email. ----------------------------------------------------------------- ------------------------------ Message: 15 Date: Fri, 4 Jan 2008 16:19:00 -0500 From: "Emily Sours" Subject: [Histonet] jackson immuno backorder To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Has anyone had Jackson Immunoresearch say their secondary antibodies are on backorder indefinitely? Another lab was told it would be an indefinite amount of time before they could get them. Emily -- "Prosperity ripened the principle of decay...and, as soon as time or accident and removed the artificial supports, the stupendous fabric yielded to the pressure of its own weight...instead of inquiring why the Roman Empire was destroyed we should rather be surprised that it has subsisted for so long" -Edward Gibbon, Decline and Fall of the Roman Empire ------------------------------ Message: 16 Date: Fri, 04 Jan 2008 12:57:11 -0800 From: Bryan Llewellyn Subject: Re: [Histonet] histochemistry an extinct art? To: Histonet Message-ID: <002801c84f14$604e65f0$05024246@yourlk4rlmsu> Content-Type: text/plain; format=flowed; charset=iso-8859-1; reply-type=original Immunohistochemistry is certainly the latest technique to coma along, and is undoubtedly of very great value. It is now in its infancy, I suspect, and I fully expect that IHC procedures will increase in usefulness as time passes. Probably some antibodies will stop being used as clinical correlation with specific antobodies becomes more standardised. However, the commonest method used in histolabs today is about 120 years old, or more. That is the H&E. There is no sign that it is going into "that long night". Much the same can be said for Masson's trichrome and its clones, the PAS histochemical procedure, Perls' histochemical procedure. How do you demonstrate reticulin, or elastic? Enzyme histochemistry is still used to demonstrate muscle fibre types. The list could go on, but I am of the opinion that the older methods still have great value and will continue to have great value for a long time to come. Sometimes they are far more convenient than IHC (toluidine blue for helicobacter, for instance), even when IHC is pushed for it. Part of the reason is that IHC and enzyme histochemistry, for instance, demonstrate different things. If an antibody against alkaline phosphatase is used, you will demonstrate the enzyme, i.e. the protein. If you do an alkaline phosphatase stain using naphthol phosphate and a diazonium salt, you demonstrate the activity of the enzyme. Those are two different things. I know I am a bit of an old fogey on this, but I always strongly recommend that histotechs become conversant with the whole range of procedures used histologically, dye staining, histochemistry and IHC. Bryan Llewellyn ----- Original Message ----- From: "Gudrun Lang" To: Sent: Friday, January 04, 2008 11:26 AM Subject: [Histonet] histochemistry an extinct art? > Hi, > > I have a question for those, that are experienced with the old and the new > techniques in histotechnology. I looked through some antiquariat-books, > that > deal a lot with histochemical demonstration of many tissue-compounds and > enzymes. These techniques sound all very strange to me and it seems like a > forgotten art. > > I work in a clinical histolab. Are the histochemical, enzymhistochemical > methods still in use in research-labs? Did immunhistochemistry replace > them > all together? Is histochemistry a part of modern cellbiology or just > obsolete? > > > > Gudrun Lang > > -------------------------------------------------------------------------------- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 17 Date: Fri, 4 Jan 2008 13:26:13 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] histochemistry an extinct art? To: Bryan Llewellyn , Histonet Message-ID: <430699.41709.qm@web61220.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Amen to all that from the bottom of my very old heart! Ren? Bryan Llewellyn wrote: Immunohistochemistry is certainly the latest technique to coma along, and is undoubtedly of very great value. It is now in its infancy, I suspect, and I fully expect that IHC procedures will increase in usefulness as time passes. Probably some antibodies will stop being used as clinical correlation with specific antobodies becomes more standardised. However, the commonest method used in histolabs today is about 120 years old, or more. That is the H&E. There is no sign that it is going into "that long night". Much the same can be said for Masson's trichrome and its clones, the PAS histochemical procedure, Perls' histochemical procedure. How do you demonstrate reticulin, or elastic? Enzyme histochemistry is still used to demonstrate muscle fibre types. The list could go on, but I am of the opinion that the older methods still have great value and will continue to have great value for a long time to come. Sometimes they are far more convenient than IHC (toluidine blue for helicobacter, for instance), even when IHC is pushed for it. Part of the reason is that IHC and enzyme histochemistry, for instance, demonstrate different things. If an antibody against alkaline phosphatase is used, you will demonstrate the enzyme, i.e. the protein. If you do an alkaline phosphatase stain using naphthol phosphate and a diazonium salt, you demonstrate the activity of the enzyme. Those are two different things. I know I am a bit of an old fogey on this, but I always strongly recommend that histotechs become conversant with the whole range of procedures used histologically, dye staining, histochemistry and IHC. Bryan Llewellyn ----- Original Message ----- From: "Gudrun Lang" To: Sent: Friday, January 04, 2008 11:26 AM Subject: [Histonet] histochemistry an extinct art? > Hi, > > I have a question for those, that are experienced with the old and the new > techniques in histotechnology. I looked through some antiquariat-books, > that > deal a lot with histochemical demonstration of many tissue-compounds and > enzymes. These techniques sound all very strange to me and it seems like a > forgotten art. > > I work in a clinical histolab. Are the histochemical, enzymhistochemical > methods still in use in research-labs? Did immunhistochemistry replace > them > all together? Is histochemistry a part of modern cellbiology or just > obsolete? > > > > Gudrun Lang > > -------------------------------------------------------------------------------- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. ------------------------------ Message: 18 Date: Fri, 04 Jan 2008 21:35:58 -0500 From: "Carrie Disbrow" Subject: [Histonet] on call coverage To: Message-ID: <477EA6BE.72AC.0059.0@shands.ufl.edu> Content-Type: text/plain; charset=US-ASCII We think our hourly pay to carry the beeper when on call is too low and we have talked to management. We supply coverage on weekends and holidays and we rotate the beeper weekly. Our employer said if we took a survey and the results revealed our on-call pay is below average we might get an increase. Would anyone like to participate in our survey? ------------------------------ Message: 19 Date: Sat, 5 Jan 2008 08:39:14 -0500 From: "Weems, Joyce" Subject: RE: [Histonet] on call coverage To: "Carrie Disbrow" , Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3204726B4B@sjhaexc02.sjha.org> Content-Type: text/plain; charset="iso-8859-1" Ours is 3.50/hr - Sundays and holidays. If called back, we have a "call back" clock code, which allows extra for travel. Good luck! j Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Carrie Disbrow Sent: Fri 1/4/2008 9:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] on call coverage We think our hourly pay to carry the beeper when on call is too low and we have talked to management. We supply coverage on weekends and holidays and we rotate the beeper weekly. Our employer said if we took a survey and the results revealed our on-call pay is below average we might get an increase. Would anyone like to participate in our survey? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Sat, 5 Jan 2008 16:43:38 +0300 From: Maxim Peshkov Subject: Re: [Histonet] immunocal To: Gayle Callis Cc: histonet@lists.utsouthwestern.edu Message-ID: <1761665308.20080105164338@mail.ru> Content-Type: text/plain; charset=us-ascii Gayle: Does this solution (4% hydrochloric acid/8% formic acid) damage to IHC in any ways or better use 15% formic acid for this purpose? We will do IHC for breast (ER, PgR, Ki67 and Her2). Maxim Peshkov Taganrog, Russia. ------------------------------ Message: 21 Date: Sat, 5 Jan 2008 11:30:44 -0500 From: "Weems, Joyce" Subject: RE: [Histonet] immunocal To: "Maxim Peshkov" , "Gayle Callis" Cc: histonet@lists.utsouthwestern.edu Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3204726B4C@sjhaexc02.sjha.org> Content-Type: text/plain; charset="iso-8859-1" Maxim, If Gayle's answer is that your solution does not work well for IHC, there is a product from BBC Biochemical that is designed for IHC - and it does work well. We use it for our bonemarrows. http://www.bbcus.com 6086 RapidCal*Immuno(tm), 1Pint 6087 RapidCal*Immuno(tm), 1 Quart 6089 RapidCal*Immuno(tm), 1 Gallon 6090 RapidCal*Immuno(tm), 1 Gallon Cube Cheers! j Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Maxim Peshkov Sent: Sat 1/5/2008 8:43 AM To: Gayle Callis Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] immunocal Gayle: Does this solution (4% hydrochloric acid/8% formic acid) damage to IHC in any ways or better use 15% formic acid for this purpose? We will do IHC for breast (ER, PgR, Ki67 and Her2). Maxim Peshkov Taganrog, Russia. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 22 Date: Sat, 5 Jan 2008 12:28:41 -0500 From: "Victoria Baker" Subject: Re: [Histonet] on call coverage To: "Carrie Disbrow" Cc: histonet@lists.utsouthwestern.edu Message-ID: <4f016b690801050928ke0a74acncb4a9f6744cca8e6@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Carrie, I've worked in different situations where on call was required and each handled it a bit differently. In one place we rotated on call/weekend service where we had a day off during the week, worked a FULL Saturday and took call for the next day. Our base was $25 for just carrying the beeper on Sunday a percentage for after hours coverage on Saturday. If we were called back in then we received automatic 2 hours OT, whether we were there for 15 minutes or the full 2 hours, any additional hours were covered at the time and a half rate. If it was a holiday and we were called back we were compensated at the holiday rate under the same guidelines. For weeknight coverage we were paid something like $2 - 3 per hour with the same OT as weekends if we were called back. This was between 1996-1999. In another position I was the lab manager and I was essentially on call 24/7 without any compensation - this meant if I was called late at night for a freezer alarm or an off hour tissue collection/animal take down, I had to come in. I was then given "compensatory time off" in lieu of $'s. In someways this worked out pretty well as I'm a single parent I used to use this time if I had to take off for a family reasons. In another lab, my staff was only on call for weekend/holiday emergency biopsy services - no frozens were done in our lab - and we usually knew on Friday night if we would have a case coming through that would require someone to come in. So coverage would begin on Saturday morning at 8am and end at 6pm Sunday night. Each tech would be paid 10% of their hourly rate for just carrying the beeper. If they were working they would get time and a half for the actual hours worked and also paid the on call as well. If called in on a holiday they recieved premium pay which is 2X their hourly wage. The average on call for 34 hours was $85 which I think was pretty fair and better than most places I'd seen given that when you take on call your weekends are pretty limited as to what you can do. Good luck! On 1/4/08, Carrie Disbrow wrote: > We think our hourly pay to carry the beeper when on call is too low and we have talked to management. We supply coverage on weekends and holidays and we rotate the beeper weekly. > Our employer said if we took a survey and the results revealed our on-call pay is below average we might get an increase. > Would anyone like to participate in our survey? > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 50, Issue 6 *************************************** From olek.michalski <@t> nencki.gov.pl Mon Jan 7 09:49:11 2008 From: olek.michalski <@t> nencki.gov.pl (Olek Michalski) Date: Mon Jan 7 09:43:50 2008 Subject: [Histonet] prolonged impregnation in Golgi-Cox (Kolb) staining In-Reply-To: <000301c83f64$0a142130$0202a8c0@HPPav2> References: <000301c83f64$0a142130$0202a8c0@HPPav2> Message-ID: Dear Peggy, thank you very much for your answer. It is pity I found it so late (something went wrong with my mailbox) because I have already gave up "saving" mentioned material. In spite of this circumstance I find it worth to discuss. I have actually processed one of these brains and tried to wash it in many changes of water but it did not give good result. I mean tissue is dark brown/green - so I could not see anything in thick sections I had cut. Cutting is another issue since the tissue is cracking badly despite soaking in 30% sucrose. I thought about using glycerin instead but I doubt it is worth trying. You mentioned air as oxidizing agent but I paid attention to fill the containers up to the cork. What could it be then? Some tissue-based oxidant? Or that the small amount of oxygen which was trapped under the containers cork? I had not expect such a massive oxidation since I used prolonged impregnation with chromates for other Golgi (w/o mercuric chloride) techniques and did not observe this effect. Finally answering your question. Golgi techniques are rather rare because they are non-specific (at least they are believed to be so). The procedure causes about 1-5% percent of the whole population of neurons to be stained but the stained cell is (almost) completely filled with crystals. So (if you are lucky) you can do nice morphology of the whole dendritic tree. I was actually thinking about MAP2 IHC but it is impossible to trace one cell's processes in such intensely stained tissue. On the other hand I would like to apply some iontophoretic intracellular injections but it is still future and really more laborious. Best regards Olek Michalski > I don't know if the tissue can be "saved", but try washing in water for a > few hours then process as usual for this technique, and see what happens > > A lot of things are working against you. The dichromate has been > oxidized, > the mercuric chloride has released a lot of hydrochloric acid which has > been > chewing up the proteins. And, boy, do you have a lot of fixative > cross-links > from both the chomate and the mercuric that you normally wouldn't have. > > As for the green color, I think that's due to the normally orangish/red > potassium dichromate Cr+6 being oxidized to green Cr+3 in an acidic > environment. The oxidizer is the air in the container, and the acidic > environment comes from hydrochloric acid being released from the mercuric > chloride during cross-linking with the tissue. > > I have a couple of questions, for anyone in the Histonet community. This > Golgi-Cox fixation procedure comes up a on occasion on Histonet, mostly > from > researchers. How do these labs dispose of the chemicals afterwards? > Mercuric > chloride cannot be dumped down any sink, and most (but not all) > water/sewer > treatment plants won't allow potassium dichromate to be disposed down the > sink either. And how do researchers dispose of the water and alcohol and > xylene in the processing afterwards, where mercuric chloride and > potassium > dichromate are being pulled out of the tissue into the processing > solutions? > > Can't these tissues be fixed in formalin (or some other less toxic > fixative > than mercury and chromium), and then IHC procedures done, such as > antibodies > against GFAP or neurofilaments or NSE? I work in a hospital, and am just > wondering why this Golgi-Cox procedure is needed by researchers, but > doesn't > seem to be needed by clinical histology laboratories. > > Peggy A. Wenk, HTL(ASCP)SLS > William Beaumont Hospital > Royal Oak, MI 48073 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Olek > Michalski > Sent: Thursday, December 13, 2007 3:31 PM > To: Histonet > Subject: [Histonet] prolonged impregnation in Golgi-Cox (Kolb) staining > > > Dear Histonetters, > > I have some brains which were left in Golgi-Cox chromation solution > (K2Cr2O7, KCrO3, HgCl2: about 1% of each) for over 6 months. I would > really > like to use this material even if staining would not be very clear. > Do you have an idea how to process the tissue in this case? Any help > would > be appreciated (and I know, next time I will be more careful about where > I > leave my preparates). > By the way, I have already cut one of these brains and I found the > sections > to be dark green. I have noticed this colour in my G-C preparates before > but > mainly on the surface. I am actually curious whether the tissue should be > more green or more red when I cut it. I mean should I shorten the normal > period of chromation (about 14-15 days) if I see the brain is getting > green? > > Yours truly > Olek Michalski -- Laboratory of Neurobiology of Development and Evolution Nencki Institute of Experimental Biology ul. Pasteura 3, 02-093 Warszawa, Poland Tel. +48 22 5892268, Fax +48 22 8225342 From japoteete <@t> saintfrancis.com Mon Jan 7 10:28:23 2008 From: japoteete <@t> saintfrancis.com (Poteete, Jacquie A.) Date: Mon Jan 7 10:28:53 2008 Subject: [Histonet] Iron pigment removal procedure Message-ID: Does anyone have a procedure for the removal of iron pigment for FFPE tissue prior to IHC staining? We have had no luck using the reference material we have available, so any help would be very much appreciated. Jacquie Poteete MT(ASCP)QIHC Lead Technologist, IHC Laboratory Saint Francis Hospital Tulsa, OK japoteete@saintfrancis.com From Jerry <@t> ralambusa.com Mon Jan 7 10:42:14 2008 From: Jerry <@t> ralambusa.com (Jerry Helisek) Date: Mon Jan 7 10:42:56 2008 Subject: [Histonet] RE: Cassette labeler interfaced Meditech Message-ID: <3855F92002259948A66A8CA2D16E3A4F05A723@server.ralambusa.com> Any of the Cassette Writers produced by RA Lamb would interface. ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ ------------------------------ Message: 4 Date: Mon, 7 Jan 2008 07:28:07 -0500 From: "Ream, Arthur" Subject: [Histonet] Cassette labeler interfaced Meditech To: Message-ID: Content-Type: text/plain; charset="us-ascii" Good Morning, Does anyone have or know of a Cassette labeler that is used with Meditech Client Server. Regards, Arthur F. Ream III Applications Analyst Cambridge Health Alliance #65 Beacon Street, Somerville MA 02143 617-665-2353 - Office 617-665-2097 - Fax aream@challiance.org From fudo <@t> ufl.edu Mon Jan 7 12:29:37 2008 From: fudo <@t> ufl.edu (FU,DONGTAO) Date: Mon Jan 7 12:30:09 2008 Subject: [Histonet] CD31 in the rat Message-ID: <1751783406.182001199730577848.JavaMail.osg@osgjas03.cns.ufl.edu> Hi, all I have the same question as Noelle asked. Our customer asked for the anti-rat CD31, too. Is anyone can give us some suggestions? Many thanks, Ann Dongtao Fu MD, Ph.D Lab Manager Molecular Pathology Core Dept. of Pathology Lab phone: 352-273-7752 Lab FAX: 352-273-7755 Lab address: D11-50 PO Box: 100275 1600 SW Archer Road University of Flodrida Gainesville, FL 32610 On Thu Jan 03 10:25:55 EST 2008, Linke_Noelle wrote: > Hi everyone! > > Has anyone found an acceptable CD31 antibody for FFPE rat tissue > since the tragic death of santa cruz's goat and their last hope > of producing an antibody that actually works?? > > Noelle > > No??lle Linke, MS, HTL(ASCP)QIHC > Allergan, Inc > 2525 Dupont Drive RD-2A > Irvine, CA 92612 > 714-246-5568 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Sandra.Harrison3 <@t> va.gov Mon Jan 7 12:36:44 2008 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Mon Jan 7 12:37:07 2008 Subject: [Histonet] black tissue marking ink Message-ID: What are those folks involved in grossing using for black marking ink? We use Black Cat India Ink, obtained at a local art store. It seems to stick fairly well to some tissue but not so great to pleural tissue, fat and serosal surfaces. We do spray vinegar to "set" the ink but on occasion the inking doesn't survive overnight tissue processing. Thanks, Sandy Harrison Histology Supervisor 612-467-2449 From Ngale <@t> bccancer.bc.ca Mon Jan 7 12:44:59 2008 From: Ngale <@t> bccancer.bc.ca (Gale, Nadia) Date: Mon Jan 7 12:45:23 2008 Subject: [Histonet] IGF1-R Message-ID: Hello Histonetters, Anyone have a protocol and antibody vendor information for IHC on ffpe human tissue for the antibody IGF1-Receptor? I'm especially interested in methods worked up on the Ventana XT instrument series, but am happy to receive any information you're willing to share. Thanks in advance! Nadia Nadia Gale Lead Histotechnologist Centre for Translational and Applied Genomics (CTAG) 3427-600 West 10th Avenue Vancouver, BC V5Z 4E6 tel 604-877-6000 ext 2426 fax 604-877-6089 ngale@bccancer.bc.ca From juditw <@t> u.washington.edu Mon Jan 7 12:50:14 2008 From: juditw <@t> u.washington.edu (Judith L. Williams) Date: Mon Jan 7 12:50:38 2008 Subject: [Histonet] embedding in agarose then paraffin Message-ID: Hi- I will be trying to embed entire mouse lungs and heart to do a 3-D reconstruction using paraffin sections stained H&E. I am thinking that filling the lungs with agarose and then embedding it in agarose will help inflate the lungs and keep cell/tissue continuity. Then I want to slice the agarose into rather thick sections - say four per lung- and put these into cassettes for paraffin processing. Each block will then be serially sectioned and slides stained and scanned for reconstruction. so questions are: 1. is Matrigel, Extragel or agarose (4 to 10%) best? 2. has anyone ever filled lungs, heart with melted agarose gel prior to embedding? 3. will the agarose- gel stay in the tissue throughout the paraffin processing? 4. will the gel stain differently in the blocks embedded in paraffin and stained with H&E? I would appreciate hearing from those of you doing whole embryos or large parts that need orientation and sectioning. Thank you! Judy Williams, HT, PhD Dept. of Comparative Medicine University of Washington From alonso.martinezcanabal <@t> utoronto.ca Mon Jan 7 12:52:28 2008 From: alonso.martinezcanabal <@t> utoronto.ca (Alonso Martinez-Canabal) Date: Mon Jan 7 12:54:07 2008 Subject: [Histonet] MEthanol-free floating Message-ID: -----Original Message----- From: Alonso Martinez-Canabal [mailto:alonso.martinezcanabal@utoronto.ca] Sent: January-07-08 1:27 PM To: 'histonet-bounces@lists.utsouthwestern.edu' Subject: MEthanol-free floating Hi, I normally work with free floating IHC In brain tissue. Normally I quench peroxidase activity with H2O2 0.3% in PBS during 30min. Allegledy, even 5min in the normal 5% concentration would cause damage because free floating sections are a little bit brittle. But what about the use with methanol to protect the tissue? I heard that methanol is used to prevent tissue damage can it be used with free floating sections? Thank you Alonso From Vickroy.Jim <@t> mhsil.com Mon Jan 7 13:04:38 2008 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Mon Jan 7 13:06:59 2008 Subject: [Histonet] WASTE URANYL ACETATE 2% AQUEOUS SOLUTION Message-ID: <24A4826E8EF0964D86BC5317306F58A504CEBB8369@mmc-mail.ad.mhsil.com> My safety officer in the hospital tells us that waste uranyl acetate is a hazardous waste and must be disposed of by a special waste hauler. I know that uranyl acetate is slightly radioactive also. We use it only for staining thin sections in electron microscopy and use less than 30 mls every two months. We use the drop-staining method. The quote from the waste hauler is astronomical. What is everybody else doing with waste uranyl acetate (2%) ? Jim Vickroy Surgical Pathology Memorial Medical Center Springfield, Illinois This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From ROrr <@t> enh.org Mon Jan 7 13:08:02 2008 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Mon Jan 7 13:08:29 2008 Subject: [Histonet] Pressure cooker. Message-ID: Hi Jo Dee, Your sears model should certainly do the job! I understand in pharma/research you may not need to have an extensively recorded QA that monitors temp on hier. You could at this point, purchase the temp strips from biocare...they are made to measure temps under pressure...like the autoclave tapes. You'll just get a single color indiciator on the strip, but it will tell you the temp got to at least 120'C or 125'C (I can't remember). At least you'll have an idea of what's going on inside the chamber, since the sears cooker most likely doesn't have a pressure gauge. Hope this helps Becky Evanston Northwestern Healthcare Hello everyone, I just want to thank all of those who responded to my pressure cooker question. I have decided to order a "3-in-1" cooker from Sears. It is able to be more flexible, it can either steam or pressure cook, as well as slow cook (which we won't be doing unless we make BBQ meatballs to go with our beer on Fridays!), so we can try different temperatures and times to optimize our protocol. I understand that the "scientific" pressure cookers are probably more accurate as far as temperature is concerned, but the price is prohibitive which is why I decided to buy the less expensive household version. Thanks for all of your help and advice, Jo Dee From gmartin <@t> marshallmedical.org Mon Jan 7 13:28:02 2008 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Mon Jan 7 13:28:25 2008 Subject: [Histonet] Equipment service Message-ID: <6ED9D4252F278841A0593D3D788AF24C017CFB31@mailsvr.MARSHMED.local> Hi histo folks, We are a small Pathology group about 40 miles East of Sacramento CA., and we are looking for a company that can service our histology labs equipment. We are not happy with our current service company. I had put a call out to them to help us hook up our phone alarm on our VIP 2000, and never got a response from them ... well low and behold ... we got caught in the last storm with a power outage, which necessitated a drive in to see if we lost power to the processor ... we did. So I think it's time to get that system running. Also has anyone ever heard of backing that unit up with some sort of power supply. As always thank you in advance. Gary From djemge <@t> aol.com Mon Jan 7 14:23:24 2008 From: djemge <@t> aol.com (djemge@aol.com) Date: Mon Jan 7 14:23:59 2008 Subject: [Histonet] c-kit (CD117) Ab & Protocol needed - 4%PFA Frozen Mouse Testes Sections Message-ID: <8CA1F8249A47B56-137C-17DC@FWM-M04.sysops.aol.com> Hello Histonetters, Please share a?working c-kit ab (company, cat. #, dilution) and detailed protocol used in your lab for 4%PFA,?30% sucrose cryoprotected, Frozen Mouse Testes sections.?We have been using an ab by Santa Cruz and haven't had much success on this tissue. I have tried our Ab at different dilutions, with 0.3% H202 quenching, with and w/out sodium citrate HEIR, blocked with goat serum. So far no luck or lots of background. Help! Donna Donna Emge, HT-ASCP Northwestern University 303 E. Superior, Lurie 7-220 Chicago, IL 60611 312-503-2036 d-emge@northwestern.edu ________________________________________________________________________ More new features than ever. Check out the new AOL Mail ! - http://webmail.aol.com From rjbuesa <@t> yahoo.com Mon Jan 7 14:29:56 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jan 7 14:30:17 2008 Subject: [Histonet] WASTE URANYL ACETATE 2% AQUEOUS SOLUTION In-Reply-To: <24A4826E8EF0964D86BC5317306F58A504CEBB8369@mmc-mail.ad.mhsil.com> Message-ID: <325647.16636.qm@web61212.mail.yahoo.com> What your safety officer suggests! Ren? J. "Vickroy, Jim" wrote: --------------------------------- Never miss a thing. Make Yahoo your homepage. From lpjones <@t> srhs-pa.org Mon Jan 7 14:38:30 2008 From: lpjones <@t> srhs-pa.org (Jones, Laura) Date: Mon Jan 7 14:38:57 2008 Subject: [Histonet] Biocare p16 Message-ID: <8E7AD740937B954F947F0DB4467EFEE056EA09@mail.srhs-pa.org> We are just wondering if anyone else has been affected by Biocare's inability to sell p16 and what you all have done to replace it? We were told by Biocare that we'd have to order the antibody (and the entire detection kit, which we don't want) from a company in Germany - MTM Labs. My Pathologist would appreciate your advice. Thanks in advance! Laura From Valerie.Hannen <@t> parrishmed.com Mon Jan 7 14:40:11 2008 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Mon Jan 7 14:40:36 2008 Subject: FW: [Histonet] on call coverage Message-ID: <5680DA93771F0C48954CC8D38425E72401AB34BF@ISMAIL.parrishmed.local> -----Original Message----- From: Hannen, Valerie Sent: Monday, January 07, 2008 3:38 PM To: 'Weems, Joyce' Subject: RE: [Histonet] on call coverage We take turns on call for weekends and holidays. We are on call 5hrs. Sat. and Sun. (7am to 12 noon). We are given $3 an hour for the on call hours. If we are called in we clock for our normal hourly wage (we are paid a minimum of 2 hrs for coming in)in addition to the call pay. Valerie Hannen Histotechnologist Parrish Medical Center Titusville,Florida -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Saturday, January 05, 2008 8:39 AM To: Carrie Disbrow; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] on call coverage Ours is 3.50/hr - Sundays and holidays. If called back, we have a "call back" clock code, which allows extra for travel. Good luck! j Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Carrie Disbrow Sent: Fri 1/4/2008 9:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] on call coverage We think our hourly pay to carry the beeper when on call is too low and we have talked to management. We supply coverage on weekends and holidays and we rotate the beeper weekly. Our employer said if we took a survey and the results revealed our on-call pay is below average we might get an increase. Would anyone like to participate in our survey? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately notify us by telephone at 321-268-6167 and return the original message to us at the listed email address. Thank you From Luis.Chiriboga <@t> med.nyu.edu Mon Jan 7 14:47:48 2008 From: Luis.Chiriboga <@t> med.nyu.edu (Luis Chiriboga) Date: Mon Jan 7 14:48:38 2008 Subject: [Histonet] NYSHS 2008 Awards Message-ID: Happy New Year Everyone!!! The NYSHS is know accepting applications for this years awards to be presented at the annual meeting in Saratoga Springs, March 2008. The award prizes can be used to attend meetings or for educational resources. Please visit the awards section of the NYSHS website to download the application. http://www.nyhisto.org/index.htm Luis Chiriboga From AnthonyH <@t> chw.edu.au Mon Jan 7 16:19:08 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Jan 7 16:21:13 2008 Subject: [Histonet] Iron pigment removal procedure Message-ID: This was a review I did for our journal a few years back. It might be usefull: Iron Histochemistry - A Review It is convenient to divide iron-containing complexes in human tissues into two categories: those in which the iron is loosely bound to proteins and easily released by mild acid treatment (eg hemosiderin) and those in which the iron is more strongly bound (masked iron) and cannot be released by mild acid hydrolysis (eg haemoglobin) (1). Iron in the body is stored in the forms of hemosiderin (ferric hydroxide polymer) or ferritin (a ferrous iron-protein complex) (1). Iron in tissues occurs mainly in the ferric state (2,3). The reactions that have been used for the detection of iron in tissues include (2-5): 1. The Quincke reaction using ammonium sulphide 2. The Perls reaction, using ferrocyanide, for ferric and the Turnbull Blue reaction, using ferricyanide for ferrous iron. 3. Coloured lakes, eg haematoxylin (Mallory's Method) 4. Coloured precipitates with organic chemicals not classified as dyes (eg bathophenanthroline). Ferric iron may be converted into ferrous iron by ammonium sulphide (Quinke's reaction) and the ferrous sulphide thus formed can then be demonstrated using the Turnbull blue reaction (3,5). Some iron-containing compounds (hemoglobin, malaria pigment, formalin pigment) do not react with the Perl's method because the iron is present in bound form. These compounds can be unmasked using hydrogen peroxide and then demonstrated using the Perl's reaction (1). Interestingly, it is possible to remove excess iron pigment from tissue sections. Iron can be removed by (5): * 15 min in 1% sodium dithionite in 0.1M acetate-HCl buffer (pH 4.5) * 3 hours in 2.4N HCl * 30 min in 3.7N H2SO4 * 15 min in 5% Oxalic acid Heavily pigmented tissues may need to have these times extended (5). References 1. Barka, T., Anderson, P.J., (1963) "Histochemistry: Theory, practice and bibliography" Harper & Row Publishers Inc, New York, p172-174. 2. Davenport, H.A., (1961) "Histological and Histochemical Technics" W.B. Saunders Co., Philadelphia, 280-284. 3. Gabe, M., (1976) "Histological Techniques" Masson, Paris, p311-317. 4. Lynch, M.J., Raphael, S., etal "Medical Laboratory Technology and Clinical Pathology" 2nd Ed, W.B Saunder Co, Philadelphia, p1135-1136. 5. Morton, D., (1978) "A comparison of iron histochemical methods for use on glycol methacrylate embedded tissues" Stain Tech 53(4):217-223. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Poteete, Jacquie A. Sent: Tuesday, 8 January 2008 3:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Iron pigment removal procedure Does anyone have a procedure for the removal of iron pigment for FFPE tissue prior to IHC staining? We have had no luck using the reference material we have available, so any help would be very much appreciated. Jacquie Poteete MT(ASCP)QIHC Lead Technologist, IHC Laboratory Saint Francis Hospital Tulsa, OK japoteete@saintfrancis.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From gayle.callis <@t> bresnan.net Mon Jan 7 16:26:49 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Mon Jan 7 16:27:08 2008 Subject: [Histonet] embedding in agarose then paraffin References: Message-ID: <007201c8517c$650f8690$6501a8c0@DHXTS541> There was a superb poster presented at an NSH meeting where they used Histogel injected into a heart for slicing with a matrix slicing device, then placing the slices into cassettes for processing. They used Histogel (from Richard Allan aka ThermoFisher Scientifc(?) which kept the heart distended for precise slicing. If you fill the lungs via a v shaped cut on top of trachea, using an 18 or larger guage needle and syringe filled with Histogel, then you should be able to can distend the lungs, but need to use only 2.5 ml or so. After filling, ans watch while you do this - overfilling with blow up alveoli - you should be able to slice a firm Histogel filled lung, and fix the slices appropriately. I don't know what happens to the gel after processing, but hopefully the gel will be replaced with paraffin, or at least infiltrated well to maintain gross morphology. Is the whole embryo question a separate question? And what do you mean by large parts, whole lungs, whole legs, whole livers????? Gayle M. Callis HT/HTL/MT(ASCP) ----- Original Message ----- From: "Judith L. Williams" To: Sent: Monday, January 07, 2008 11:50 AM Subject: [Histonet] embedding in agarose then paraffin > > Hi- I will be trying to embed entire mouse lungs and heart to do a 3-D > reconstruction using paraffin sections stained H&E. I am thinking that > filling the lungs with agarose and then embedding it in agarose will help > inflate the lungs and keep cell/tissue continuity. Then I want to slice > the agarose into rather thick sections - say four per lung- and put these > into cassettes for paraffin processing. Each block will then be serially > sectioned and slides stained and scanned for reconstruction. > so questions are: > 1. is Matrigel, Extragel or agarose (4 to 10%) best? > 2. has anyone ever filled lungs, heart with melted agarose gel prior to > embedding? > 3. will the agarose- gel stay in the tissue throughout the paraffin > processing? > 4. will the gel stain differently in the blocks embedded in paraffin and > stained with H&E? > I would appreciate hearing from those of you doing whole embryos or large > parts that need orientation and sectioning. Thank you! > > Judy Williams, HT, PhD > Dept. of Comparative Medicine > University of Washington > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sheila_adey <@t> hotmail.com Tue Jan 8 06:59:33 2008 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Tue Jan 8 06:59:59 2008 Subject: [Histonet] Equipment service In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C017CFB31@mailsvr.MARSHMED.local> References: <6ED9D4252F278841A0593D3D788AF24C017CFB31@mailsvr.MARSHMED.local> Message-ID: We have our VIP5s on an emergency back up plug. We also purchased an alarm from Radio Shack and if the power fails on the processor, it calls another area in the lab. Sheila Adey HT MLTPort Huron HospitalMichigan> Date: Mon, 7 Jan 2008 11:28:02 -0800> From: gmartin@marshallmedical.org> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] Equipment service> > Hi histo folks,> > We are a small Pathology group about 40 miles East of Sacramento CA.,> and we are looking for a company that can service our histology labs> equipment. We are not happy with our current service company. I had> put a call out to them to help us hook up our phone alarm on our VIP> 2000, and never got a response from them ... well low and behold ... we> got caught in the last storm with a power outage, which necessitated a> drive in to see if we lost power to the processor ... we did. So I> think it's time to get that system running. > > > > Also has anyone ever heard of backing that unit up with some sort of> power supply. > > As always thank you in advance. > > Gary> > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Read what Santa`s been up to! For all the latest, visit asksantaclaus.spaces.live.com! http://asksantaclaus.spaces.live.com/ From Anthony.Gatt <@t> jefferson.edu Tue Jan 8 07:58:44 2008 From: Anthony.Gatt <@t> jefferson.edu (Anthony Gatt) Date: Tue Jan 8 07:51:29 2008 Subject: [Histonet] cracked samples Message-ID: <20080108085844.AMW40485@logan.jefferson.edu> Hello, I have been paraffin sectioning drosophila heads at 6 microns. After H&E staining and observing them under a microscope, I notice that some sections have cracks in them. Sometimes the cracks are so bad that the sample is unusable. However, often in the same block some of the samples are perfect. Any ideas? Thanks. From settembr <@t> umdnj.edu Tue Jan 8 07:57:43 2008 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Tue Jan 8 07:58:38 2008 Subject: [Histonet] c-kit (CD117) Ab & Protocol needed - 4%PFA Frozen Mouse Testes Sections Message-ID: Hello Donna, I am sorry I use c-kit on human paraffin tissue and if it might help I use Cell Marque's c-kit cat# CMD768 @ 1:100 dilution and Dako's Env+ Rabbit detection kit. I use Triliogy HIER, but as I stated I use it on paraffin section. I wouldn't use it on frozens. Good Luck, Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> 01/07/08 3:23 PM >>> Hello Histonetters, Please share a?working c-kit ab (company, cat. #, dilution) and detailed protocol used in your lab for 4%PFA,?30% sucrose cryoprotected, Frozen Mouse Testes sections.?We have been using an ab by Santa Cruz and haven't had much success on this tissue. I have tried our Ab at different dilutions, with 0.3% H202 quenching, with and w/out sodium citrate HEIR, blocked with goat serum. So far no luck or lots of background. Help! Donna Donna Emge, HT-ASCP Northwestern University 303 E. Superior, Lurie 7-220 Chicago, IL 60611 312-503-2036 d-emge@northwestern.edu ________________________________________________________________________ More new features than ever. Check out the new AOL Mail ! - http://webmail.aol.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue Jan 8 07:58:44 2008 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Jan 8 07:59:11 2008 Subject: [Histonet] cracked samples Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F421@wahtntex2.waht.swest.nhs.uk> Try leaving all the sections on the water bath for the same time as those that are perfect; maybe those left on too long are the ones that are cracking? Kemlo Rogerson Pathology Manager and AHP Lead DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Don't be afraid to take a big step when one is indicated. You can't cross a chasm in two small jumps. --Buckminster Fuller This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From settembr <@t> umdnj.edu Tue Jan 8 07:59:06 2008 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Tue Jan 8 08:00:06 2008 Subject: [Histonet] Biocare p16 Message-ID: We are in the same boat. I guess we all will be. We didn't want to use the entire detection kit either. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> "Jones, Laura" 01/07/08 3:38 PM >>> We are just wondering if anyone else has been affected by Biocare's inability to sell p16 and what you all have done to replace it? We were told by Biocare that we'd have to order the antibody (and the entire detection kit, which we don't want) from a company in Germany - MTM Labs. My Pathologist would appreciate your advice. Thanks in advance! Laura _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Tue Jan 8 08:22:35 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Jan 8 08:22:57 2008 Subject: [Histonet] jackson immuno backorder In-Reply-To: <4FE7FB862E90E448AE32388E759220E50861A5@pbrcas31.pbrc.edu> References: <4FE7FB862E90E448AE32388E759220E50861A5@pbrcas31.pbrc.edu> Message-ID: Update on the backorder: only one of the antibodies we use are backordered and that's only for two days. I have a feeling this was like a run on the bank--someone's antibody was backordered for longer than two days, everyone panicked and spread the rumor that everything was backordered. Emily who just read the Studs Lonighan trilogy, so depression-era metaphors come easily -- "Prosperity ripened the principle of decay...and, as soon as time or accident and removed the artificial supports, the stupendous fabric yielded to the pressure of its own weight...instead of inquiring why the Roman Empire was destroyed we should rather be surprised that it has subsisted for so long" -Edward Gibbon, Decline and Fall of the Roman Empire From algranth <@t> u.arizona.edu Tue Jan 8 08:26:19 2008 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Tue Jan 8 08:33:15 2008 Subject: [Histonet] embedding in agarose then paraffin In-Reply-To: <007201c8517c$650f8690$6501a8c0@DHXTS541> References: <007201c8517c$650f8690$6501a8c0@DHXTS541> Message-ID: <6.2.3.4.1.20080108071852.01f9ce58@algranth.inbox.email.arizona.edu> Histogel processes along with the tissue and should cut fine. I haven't injected it into a lung or heart but I think it might work. I've embedded tissues and other types of samples (like collagen fibers) in histogel with good results. Helps with orientation. The gel does not get replaced with paraffin and cuts fine. After staining there might be a sort of halo around the tissue where the histogel is but I haven't found that it interferes with the tissue when you look at it under the microscope. Andi Grantham At 03:26 PM 1/7/2008, Gayle Callis wrote: >There was a superb poster presented at an NSH meeting where they >used Histogel injected into a heart for slicing with a matrix >slicing device, then placing the slices into cassettes for >processing. They used Histogel (from Richard Allan aka >ThermoFisher Scientifc(?) which kept the heart distended for precise >slicing. If you fill the lungs via a v shaped cut on top of >trachea, using an 18 or larger guage needle and syringe filled with >Histogel, then you should be able to can distend the lungs, but need >to use only 2.5 ml or so. After filling, ans watch while you do >this - overfilling with blow up alveoli - you should be able to >slice a firm Histogel filled lung, and fix the slices appropriately. > >I don't know what happens to the gel after processing, but hopefully >the gel will be replaced with paraffin, or at least infiltrated well >to maintain gross morphology. > >Is the whole embryo question a separate question? And what do you >mean by large parts, whole lungs, whole legs, whole livers????? > >Gayle M. Callis >HT/HTL/MT(ASCP) > > >----- Original Message ----- From: "Judith L. Williams" > >To: >Sent: Monday, January 07, 2008 11:50 AM >Subject: [Histonet] embedding in agarose then paraffin > > >> >>Hi- I will be trying to embed entire mouse lungs and heart to do a >>3-D reconstruction using paraffin sections stained H&E. I am >>thinking that filling the lungs with agarose and then embedding it >>in agarose will help inflate the lungs and keep cell/tissue >>continuity. Then I want to slice the agarose into rather thick >>sections - say four per lung- and put these into cassettes for >>paraffin processing. Each block will then be serially sectioned and >>slides stained and scanned for reconstruction. >>so questions are: >>1. is Matrigel, Extragel or agarose (4 to 10%) best? >>2. has anyone ever filled lungs, heart with melted agarose gel >>prior to embedding? >>3. will the agarose- gel stay in the tissue throughout the paraffin >>processing? >>4. will the gel stain differently in the blocks embedded in >>paraffin and stained with H&E? >>I would appreciate hearing from those of you doing whole embryos or >>large parts that need orientation and sectioning. Thank you! >> >>Judy Williams, HT, PhD >>Dept. of Comparative Medicine >>University of Washington >> >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From algranth <@t> u.arizona.edu Tue Jan 8 08:32:35 2008 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Tue Jan 8 08:39:23 2008 Subject: [Histonet] cracked samples Message-ID: <6.2.3.4.1.20080108073142.01f99550@algranth.inbox.email.arizona.edu> Actually I meant to send this to the list and not just to Kemlo: To: "Kemlo Rogerson" Subject: RE: [Histonet] cracked samples Could it be that you caused the cracks in the some of the samples and not in others when you obtained the samples? I understood that both cracked and non-cracked samples were in the same block (would be in the same section of paraffin) so leaving on the waterbath is not going to help. Andi Grantham ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From anh2006 <@t> med.cornell.edu Tue Jan 8 09:49:07 2008 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Tue Jan 8 09:49:44 2008 Subject: [Histonet] jackson immuno backorder Message-ID: I have been waiting for donkey anti-mouse IgG CY2 for three months from Jackson Immuno so I consider the backorder issue fairly significant. I am on the edge of doing what I thought I would never do and order from someone else. ----- Original Message ----- From: Emily Sours Date: Tuesday, January 8, 2008 9:22 am Subject: Re: [Histonet] jackson immuno backorder > Update on the backorder: only one of the antibodies we use are > backordered and that's only for two days. I have a feeling this was > like a run on the bank--someone's antibody was backordered for longer > than two days, everyone panicked and spread the rumor that everything > was backordered. > > Emily From burch007 <@t> mc.duke.edu Tue Jan 8 12:30:16 2008 From: burch007 <@t> mc.duke.edu (James L Burchette) Date: Tue Jan 8 12:28:35 2008 Subject: [Histonet] Jackson Immuno back-order In-Reply-To: Message-ID: Dear All I ordered Cy-2 labeled goat anti-mouse IgG from Jackson several months ago. When I called to inquire about availability, they told me there was a stocking problem with their supplier of certain fluorochromes. I received my back ordered cy-2 anti- mouse yesterday. Maybe the problem is solved. This is the first time I have ever had a problem with Jackson since I started using their products 20 years ago. I feel they were straight forward and honest with me and this was a problem out of their control. JB Jim Burchette, HT(ASCP) QIHC "A simple histotech from a little country hospital in North Carolina" "Andrea Hooper" Sent by: histonet-bounces@lists.utsouthwestern.edu 01/08/2008 10:49 AM To histonet@lists.utsouthwestern.edu cc Subject Re: [Histonet] jackson immuno backorder I have been waiting for donkey anti-mouse IgG CY2 for three months from Jackson Immuno so I consider the backorder issue fairly significant. I am on the edge of doing what I thought I would never do and order from someone else. ----- Original Message ----- From: Emily Sours Date: Tuesday, January 8, 2008 9:22 am Subject: Re: [Histonet] jackson immuno backorder > Update on the backorder: only one of the antibodies we use are > backordered and that's only for two days. I have a feeling this was > like a run on the bank--someone's antibody was backordered for longer > than two days, everyone panicked and spread the rumor that everything > was backordered. > > Emily _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From benoit.delatour <@t> u-psud.fr Tue Jan 8 12:44:01 2008 From: benoit.delatour <@t> u-psud.fr (=?ISO-8859-1?Q?Delatour_Beno=EEt?=) Date: Tue Jan 8 12:44:31 2008 Subject: [Histonet] Re: MEthanol-free floating (Alonso Martinez-Canabal) In-Reply-To: <20080108182418.D5AD26E0798@smtp1.u-psud.fr> References: <20080108182418.D5AD26E0798@smtp1.u-psud.fr> Message-ID: <4783C471.101@u-psud.fr> From: Alonso Martinez-Canabal [[1]mailto:alonso.martinezcanabal@utoronto.ca] Sent: January-07-08 1:27 PM To: '[2]histonet-bounces@lists.utsouthwestern.edu' Subject: MEthanol-free floating Hi, I normally work with free floating IHC In brain tissue. Normally I quench peroxidase activity with H2O2 0.3% in PBS during 30min. Allegledy, even 5min in the normal 5% concentration would cause damage because free floating sections are a little bit brittle. But what about the use with methanol to protect the tissue? I heard that methanol is used to prevent tissue damage can it be used with free floating sections? Thank you Alonso Hi Alonso, I used for years this protocol on free floating sections, without significant damage to the tissue: -incubate tissue in this quenching solution for 10 min: 2 ml methanol +8ml H2O dist. + 300 ?l of 30% H[2]O[2] -rinse 5 min in H20 dist and then in buffer (eg PBS) till all bubbles are removed. This treatment is very efficient to block endogeneous perox. activity Good luck! B.Delatour References 1. mailto:alonso.martinezcanabal@utoronto.ca 2. mailto:histonet-bounces@lists.utsouthwestern.edu From anh2006 <@t> med.cornell.edu Tue Jan 8 12:44:22 2008 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Tue Jan 8 12:44:49 2008 Subject: [Histonet] Jackson Immuno back-order In-Reply-To: References: Message-ID: I don't doubt it, they are a fabulous company. I was informed yesterday mine would not arrive until February. Andrea At 1:30 PM -0500 1/8/08, James L Burchette wrote: >Dear All >I ordered Cy-2 labeled goat anti-mouse IgG from Jackson several >months ago. When I called to inquire about availability, they told >me there was a stocking problem with their supplier of certain >fluorochromes. I received my back ordered cy-2 anti- mouse >yesterday. Maybe the problem is solved. This is the first time I >have ever had a problem with Jackson since I started using their >products 20 years ago. I feel they were straight forward and honest >with me and this was a problem out of their control. >JB > >Jim Burchette, HT(ASCP) QIHC >"A simple histotech from a little country hospital in North Carolina" > >"Andrea Hooper" >Sent by: histonet-bounces@lists.utsouthwestern.edu > >01/08/2008 10:49 AM >To >histonet@lists.utsouthwestern.edu >cc >Subject >Re: [Histonet] jackson immuno backorder > > > > > > >I have been waiting for donkey anti-mouse IgG CY2 for three months >from Jackson Immuno so I consider the backorder issue fairly >significant. I am on the edge of doing what I thought I would never >do and order from someone else. > >----- Original Message ----- >From: Emily Sours >Date: Tuesday, January 8, 2008 9:22 am >Subject: Re: [Histonet] jackson immuno backorder > >> Update on the backorder: only one of the antibodies we use are >> backordered and that's only for two days. I have a feeling this was >> like a run on the bank--someone's antibody was backordered for longer >> than two days, everyone panicked and spread the rumor that everything >> was backordered. >> >> Emily > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- From carl.hobbs <@t> kcl.ac.uk Tue Jan 8 12:55:29 2008 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Tue Jan 8 12:56:11 2008 Subject: [Histonet] Re: C-kit CD117 Message-ID: <000501c85228$0a345370$4001a8c0@carlba65530bda> Hi Donna. We tried to get sc5535 to work on complete and disassociated mouse intestinal muscle layer, mouse frozen gut ( unfixed and fixed-fixed) and on pwax sections of same...all negative. Only one we got to work on each, although it was best on pwax sections, was Dako's anti c-kit A4502 , 1/3-500 dilution factor. Try it on your frozens: also try pwax sections. good luck. Carl From Benjamin.Kriederman <@t> providence.org Tue Jan 8 13:51:26 2008 From: Benjamin.Kriederman <@t> providence.org (Kriederman, Ben) Date: Tue Jan 8 13:51:51 2008 Subject: [Histonet] Dako Autostainer plus issues Message-ID: Hi all, Has anyone used the pap pen with the dako autostainer plus to avoid the fluid from shrinking up on the slides and exposing dry portions of tissue? If so what has been your experience with the pen. What volumes do you use in the drop zones of your staining protocols? Thanks for the help, Ben DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From hej01 <@t> health.state.ny.us Tue Jan 8 13:51:44 2008 From: hej01 <@t> health.state.ny.us (Helen E Johnson) Date: Tue Jan 8 13:52:09 2008 Subject: [Histonet] decal mouse feet & IHC Message-ID: Hi Histonetters, How are you decaling mouse feet for IHC? Helen Johnson (hej01@health.state.ny.us) IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. From liz <@t> premierlab.com Tue Jan 8 14:06:08 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Jan 8 14:06:35 2008 Subject: [Histonet] Dako Autostainer plus issues In-Reply-To: <6A46880EE8EB48E2ADB4E7A1984318A3@PremierLab.local> References: <6A46880EE8EB48E2ADB4E7A1984318A3@PremierLab.local> Message-ID: Ben We use a pap pen all the time on the dako autostainer. We place our tissues on the lower portion of the slides so we are primarily using drop zones 1 and 2. If the tissue is small enought that one drop zone will cover it we will use drop zone one with a volume of 150ul. If the tissue spans 2 drop zones we use drop zones 1 and 2 with a volume of 100ul. And if we have tissue that covers the entire slide, we use 3 drop zones with 100ul per drop zone. We place the pap pen above the tissue just one line, occasionly we get slides in from clients that have tissues placed all over the slide or in the middle, we will then use drop zones 2 or 3 and place a pap pen line on the top and bottom of the tissue. This works well for us. Just make sure that the pap pen line is not too close to the tissue. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kriederman, Ben Sent: Tuesday, January 08, 2008 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dako Autostainer plus issues Hi all, Has anyone used the pap pen with the dako autostainer plus to avoid the fluid from shrinking up on the slides and exposing dry portions of tissue? If so what has been your experience with the pen. What volumes do you use in the drop zones of your staining protocols? Thanks for the help, Ben DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Jan 8 14:12:46 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 8 14:13:11 2008 Subject: [Histonet] Dako Autostainer plus issues In-Reply-To: Message-ID: <227194.82623.qm@web61223.mail.yahoo.com> INSTEAD of the pen I used a "conventional" WAX pencil (either blue or red) with very good results (with the DAKO autostainer). Ren? J. "Kriederman, Ben" wrote: http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From AGrobe2555 <@t> aol.com Tue Jan 8 14:19:13 2008 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Tue Jan 8 14:19:33 2008 Subject: [Histonet] GAG/Alcian blue - Staining intensity Message-ID: Hello all, We have done a GAG stain using the Alcian blue pH 2.5 on our slides. The GAGs appear to be staining an "aquamarine" color of varying intensity. Is this correct? Is there any way to darken the color? Thanks, Albert **************Start the year off right. Easy ways to stay in shape. http://body.aol.com/fitness/winter-exercise?NCID=aolcmp00300000002489 From rjbuesa <@t> yahoo.com Tue Jan 8 14:36:15 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 8 14:36:38 2008 Subject: [Histonet] GAG/Alcian blue - Staining intensity In-Reply-To: Message-ID: <583489.78396.qm@web61222.mail.yahoo.com> The tone will depend on the pH; check the pH of your solution against your recipe. The lower the pH the fainter the blue is. Ren? J. AGrobe2555@aol.com wrote: --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From Jessica.Vacca <@t> HCAhealthcare.com Tue Jan 8 14:36:45 2008 From: Jessica.Vacca <@t> HCAhealthcare.com (Vacca Jessica) Date: Tue Jan 8 14:37:25 2008 Subject: [Histonet] Gold chloride/silver nitrate trap Message-ID: <41E16A15CE78374EA45B57E0F94339B803320E58@ORLEV01.hca.corpad.net> Happy New Year! My department is in the process of getting a quote for a remodeling that has been long over due. My plant ops guys were just here and asked me about a gold chloride/silver nitrate trap that is attached to our draining system. (I'm in FL by the way) Does anyone know the necessity of this device? We do use both chemicals. Thanks Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr. Brandon Fl 33511 (813) 571-5193 or (813) 681-5551 ext 2454 Jessica.Vacca@hcahealthcare.com From gayle.callis <@t> bresnan.net Tue Jan 8 15:48:26 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Tue Jan 8 15:48:44 2008 Subject: [Histonet] decal mouse feet & IHC References: Message-ID: <001c01c85240$331d9360$6501a8c0@DHXTS541> If the antigen is not damaged by acids, then buffered formic acid should work using endpoint determination to ensure no overexposure to the acid. Normally we do 14% tetrasodium EDTA in Dulbeccos PBS at pH 7.4 - 7.6, the pH range of TRIS buffered saline. WE do adjust the pH down with glacial acetic acid using constant stirring and a pH meter. The decalcification may take a week or more, once again tested with endpoint determination, the weight gain/weight loss method or FAXITRON xray method. Mouse paws take longer to fix with NBF than you think, unless you start with perfusion fixation followed by immersion. We have fixed for up to a week since the bones are very packed very closed together with skin and tendons involved. Gayle M. Callis HT/HTL/MT(ASCP) ----- Original Message ----- From: "Helen E Johnson" To: Sent: Tuesday, January 08, 2008 12:51 PM Subject: [Histonet] decal mouse feet & IHC > > Hi Histonetters, > How are you decaling mouse feet for IHC? > Helen Johnson (hej01@health.state.ny.us) > From bhewlett <@t> cogeco.ca Tue Jan 8 15:51:06 2008 From: bhewlett <@t> cogeco.ca (bhewlett@cogeco.ca) Date: Tue Jan 8 15:51:32 2008 Subject: [Histonet] GAG/Alcian blue - Staining intensity Message-ID: <4783f04a.2af.72c3.2009@cogeco.ca> > Sorry Rene, but I must disagree. Lowering the pH of AB solutions does NOT change the shade of blue obtained (check the solution with a spectrophotometer), it DOES restrict the reaction to more highly sulphated GAGS and since there may be fewer of these sites then the overall intensity may be reduced, but not the shade of colour. I can show pictures of Mast cell granules stained at less than pH0.5 that are an intense blue.If the intensity of the GAG/AB complex is pale then the best approach is to increase staining time to allow more dye molecules to agregate. Bryan > The tone will depend on the pH; check the pH of your solution against your recipe. The lower the pH the fainter the > blue is. > Ren? J. > > AGrobe2555@aol.com wrote: > > > > > --------------------------------- > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JCT70 <@t> CLASSICNET.NET Tue Jan 8 15:57:43 2008 From: JCT70 <@t> CLASSICNET.NET (Jose Taramona) Date: Tue Jan 8 15:58:12 2008 Subject: [Histonet] Looking for alpha-napthyl butyrate esterase activity (Non-Specific Esterase) procedure Message-ID: <000001c85241$809fd750$0401a8c0@Jose> Hello, Our hem-path would like us to start doing the alpha-napthyl butyrate esterase in peripheral blood and bone marrow smears. We are having problems and would like any input. We were trying to use a sigma kit. Thanks Jose Taramona. From gmartin <@t> marshallmedical.org Tue Jan 8 16:35:13 2008 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Tue Jan 8 16:35:39 2008 Subject: [Histonet] Back up Message-ID: <6ED9D4252F278841A0593D3D788AF24C018096C4@mailsvr.MARSHMED.local> thank you to all who responded to my request for information concerning ,how to backup our processor during a power failure. Most of the suggestions involved a UPS battery backup, which seems to be part of the solution. However a battery backup will only provide a small amount of backup time (ex. 1 battery = 5min ... additional batteries will provide more mins). We are a standalone lab that is not inside a bigger facility so all solutions will need to be accomplished by myself and one other person, which can be accomplished (I believe), with the UPS, and the phone alarm. This brings me to the first part of my original question; does anyone know of a California company that can assist me in programming my Tissue Tek VIP 2000 phone alarm, and provide yearly maintenance on all of my histology lab equipment. Gary From annrrob <@t> aol.com Tue Jan 8 16:45:21 2008 From: annrrob <@t> aol.com (annrrob@aol.com) Date: Tue Jan 8 16:45:48 2008 Subject: [Histonet] (no subject) Message-ID: <8CA205F48A6A2F0-BA4-E80@FWM-D03.sysops.aol.com> Does anyone else use avidin biotin with the Biogenex QD200-OX kit.? Biogenex tells me it is not necessary to use this block, but others tell me avidin biotin must be used on all tissues regardless of biotin activity.? Biogenex says the power block is universal and does not require avidin biotin block unless there is biotin proven background staining.? I am so confused.? Please help. ________________________________________________________________________ More new features than ever. Check out the new AOL Mail ! - http://webmail.aol.com From JWEEMS <@t> sjha.org Tue Jan 8 16:47:58 2008 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Jan 8 16:48:34 2008 Subject: [Histonet] Back up In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C018096C4@mailsvr.MARSHMED.local> References: <6ED9D4252F278841A0593D3D788AF24C018096C4@mailsvr.MARSHMED.local> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F482A@sjhaexc02.sjha.org> I would call Sakura. They will direct you I'm sure. You can even get an alarm that will call you when there is a problem. Good luck! J -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Gary Sent: Tuesday, January 08, 2008 5:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Back up thank you to all who responded to my request for information concerning ,how to backup our processor during a power failure. Most of the suggestions involved a UPS battery backup, which seems to be part of the solution. However a battery backup will only provide a small amount of backup time (ex. 1 battery = 5min ... additional batteries will provide more mins). We are a standalone lab that is not inside a bigger facility so all solutions will need to be accomplished by myself and one other person, which can be accomplished (I believe), with the UPS, and the phone alarm. This brings me to the first part of my original question; does anyone know of a California company that can assist me in programming my Tissue Tek VIP 2000 phone alarm, and provide yearly maintenance on all of my histology lab equipment. Gary _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tim.morken <@t> thermofisher.com Tue Jan 8 17:27:05 2008 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Tue Jan 8 17:27:48 2008 Subject: [Histonet] Endogenous biotin In-Reply-To: <8CA205F48A6A2F0-BA4-E80@FWM-D03.sysops.aol.com> References: <8CA205F48A6A2F0-BA4-E80@FWM-D03.sysops.aol.com> Message-ID: <6BFF6D137DF6BC43B33891BA96E83B19010DA32D@PGHCR-EXMB-VS-1.na.fshrnet.com> If you are using a biotinylated secondary, then you should use avidin-biotin blocking. Most of your tissues will not show endogenous biotin, but there is evidence that antigen retrieval uncovers a lot more endogenous biotin than is usually understood to be around. Conventional knowledge says you only need it for certain tissues, but you can see it in many tissues with AR treatment - especially in tumor tissue. The alternative is to 1) be very attentive to your negative controls compared to your test tissue to rule out false positive due to endogenous biotin (and document the results) or 2) use a biotin-free detection kit. See this article for some basic information: http://www.propathlab.com/pdf/Focus_2004-01_endog_biotin.pdf Tim Morken Technical Support Manager Lab Vision Products Anatomical Pathology ThermoFisher Scientific -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of annrrob@aol.com Sent: Tuesday, January 08, 2008 2:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Does anyone else use avidin biotin with the Biogenex QD200-OX kit.? Biogenex tells me it is not necessary to use this block, but others tell me avidin biotin must be used on all tissues regardless of biotin activity.? Biogenex says the power block is universal and does not require avidin biotin block unless there is biotin proven background staining.? I am so confused.? Please help. ________________________________________________________________________ More new features than ever. Check out the new AOL Mail ! - http://webmail.aol.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Warren_Eddings <@t> ssmhc.com Tue Jan 8 17:33:31 2008 From: Warren_Eddings <@t> ssmhc.com (Warren_Eddings@ssmhc.com) Date: Tue Jan 8 17:34:14 2008 Subject: [Histonet] antibody validation Message-ID: hello does anyone thanks warren_eddings@ssmhc _________________________________________________________________ Con attachments, is for may contain confidential and unauthorized review, use, disclosure or distr prohibited. If you are not the intended recipient, please cont the sender by reply email and destroy all copies of the original messag From david.kinsley <@t> spcorp.com Tue Jan 8 17:50:29 2008 From: david.kinsley <@t> spcorp.com (Kinsley, David) Date: Tue Jan 8 17:50:54 2008 Subject: [Histonet] CD11c on paraffin? Message-ID: Hi, Does anyone know of a CD11c antibody that works on FFPE tissue sections? I'm looking for antibodies that work on either human or mouse tissues. My samples are fixed in formalin for 24 hours then transferred to 70% ETOH and processed into paraffin. Thanks in advance Dave ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From cfitz <@t> telus.net Tue Jan 8 20:56:10 2008 From: cfitz <@t> telus.net (Cathy) Date: Tue Jan 8 20:56:35 2008 Subject: [Histonet] Biocare p16 In-Reply-To: <8E7AD740937B954F947F0DB4467EFEE056EA09@mail.srhs-pa.org> References: <8E7AD740937B954F947F0DB4467EFEE056EA09@mail.srhs-pa.org> Message-ID: <000601c8526b$30c08200$6401a8c0@your8ba846406f> We were notified last October by Cell Marque that MTM Labs had decided to market p16 themselves. I have spoken with their rep here in North America and was told that we would have to purchase the entire kit but would be able to use only the antibody (we have Ventana Benchmarks). The cost per test will double but our pathologists have decided that we still need to have p16 available. The rest of the kit will end up being discarded. Cathy Kelowna, B.C. Canada -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jones, Laura Sent: Monday, January 07, 2008 12:39 PM To: Histonet (E-mail) Subject: [Histonet] Biocare p16 We are just wondering if anyone else has been affected by Biocare's inability to sell p16 and what you all have done to replace it? We were told by Biocare that we'd have to order the antibody (and the entire detection kit, which we don't want) from a company in Germany - MTM Labs. My Pathologist would appreciate your advice. Thanks in advance! Laura _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.5.516 / Virus Database: 269.17.13/1211 - Release Date: 1/6/2008 11:57 AM No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.5.516 / Virus Database: 269.17.13/1214 - Release Date: 1/8/2008 1:38 PM From Histopatty <@t> aol.com Tue Jan 8 21:15:39 2008 From: Histopatty <@t> aol.com (Histopatty@aol.com) Date: Tue Jan 8 21:16:08 2008 Subject: [Histonet] Histology Tracking System Message-ID: Are there any tracking systems for Surgical pathology out there? I am aware of two, one is private and the other is in the making. We have meditech. Patty Eneff OUMC 405-271-5653 ------------------------------ _______________________________________________ **************Start the year off right. Easy ways to stay in shape. http://body.aol.com/fitness/winter-exercise?NCID=aolcmp00300000002489 From jnocito <@t> satx.rr.com Wed Jan 9 01:38:00 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Jan 9 01:37:26 2008 Subject: [Histonet] Biocare p16 References: <8E7AD740937B954F947F0DB4467EFEE056EA09@mail.srhs-pa.org> <000601c8526b$30c08200$6401a8c0@your8ba846406f> Message-ID: <00e301c85292$8ff74930$0302a8c0@yourxhtr8hvc4p> Flame time See, this is what just fry my cookies about some of these vendors. Tell me this isn't a money issue. What happened to patient care, or is that obsolete now? I hate it when some one has a good product, then screws it up by getting greedy about it. Now, you have to purchase the detection kit to get the antibody. Bull do-do. Now I'm really urinated off. Get me the CEO. As some of you know, I have no qualms about getting into the face of a CEO. I had plenty of experience at my last job, not to mention a couple of NSH meetings too. JTT ----- Original Message ----- From: "Cathy" To: "'Jones, Laura'" ; "'Histonet (E-mail)'" Sent: Tuesday, January 08, 2008 8:56 PM Subject: RE: [Histonet] Biocare p16 > > We were notified last October by Cell Marque that MTM Labs had decided to > market p16 themselves. I have spoken with their rep here in North America > and was told that we would have to purchase the entire kit but would be > able > to use only the antibody (we have Ventana Benchmarks). The cost per test > will double but our pathologists have decided that we still need to have > p16 > available. The rest of the kit will end up being discarded. > > Cathy > Kelowna, B.C. > Canada > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jones, > Laura > Sent: Monday, January 07, 2008 12:39 PM > To: Histonet (E-mail) > Subject: [Histonet] Biocare p16 > > We are just wondering if anyone else has been affected by Biocare's > inability to sell p16 and what you all have done to replace it? We were > told by Biocare that we'd have to order the antibody (and the entire > detection kit, which we don't want) from a company in Germany - MTM Labs. > My Pathologist would appreciate your advice. Thanks in advance! Laura > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.5.516 / Virus Database: 269.17.13/1211 - Release Date: 1/6/2008 > 11:57 AM > > > No virus found in this outgoing message. > Checked by AVG Free Edition. > Version: 7.5.516 / Virus Database: 269.17.13/1214 - Release Date: 1/8/2008 > 1:38 PM > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MICHELLE.MCNEESE <@t> childrens.com Wed Jan 9 07:39:35 2008 From: MICHELLE.MCNEESE <@t> childrens.com (MICHELLE MCNEESE) Date: Wed Jan 9 07:40:08 2008 Subject: [Histonet] Response to On-Call pay Message-ID: <47847A37020000290000F772@CNET3.CHILDRENS.COM> We are paid $3/hour starting at 5:00 pm Friday evening and ending at Midnight on Sunday night (Monday morning). If we are called in, we get a minimum 3 hour call back pay and 1 and a half times our hourly pay rate, plus a $5/hour weekend differential rate. Histotechs rotate call every week-end from Friday to Monday. As the lead tech, I cover all call from Monday to Thursday. We come in for equipment problems and rapid processing requests and are available for answering questions. Pathologists come in on their own for frozen sections, etc. Hope this helps! We had to really fight to get our on-call pay equivalent to the call that we were actually doing...so don't give up! Michelle L McNeese, HT (ASCP) Lead Tech, Histology Department of Pathology Childrens Medical Center (214) 456-6146 Email: michelle.mcneese@childrens.com From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Jan 9 08:06:41 2008 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Jan 9 08:07:07 2008 Subject: [Histonet] Response to On-Call pay Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F446@wahtntex2.waht.swest.nhs.uk> $3 per hour???? That's about 50p at current exchange rates; I really don't know how you Americans can afford to live. I mean a nice bottle of Chardonnay or Chablis must be a weeks wages. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Don't be afraid to take a big step when one is indicated. You can't cross a chasm in two small jumps. --Buckminster Fuller This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From gayle.callis <@t> bresnan.net Wed Jan 9 09:17:48 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Wed Jan 9 09:18:07 2008 Subject: [Histonet] CD11c on paraffin? References: Message-ID: <002301c852d2$cb497d60$6501a8c0@DHXTS541> David, We use BDPharmingen ala Invitrogen rat anti mouse CD11c on murine tissue, but only on frozen sections for both immunhistochemistry and immunofluorescence. This antibody proved to be far superior to another we were using and you can access the BD product data sheet online to see what they say about its use with FFPE. Have you looked in Histonet Archives as this question has been asked before. Gayle M. Callis HT/HTL/MT(ASCP) Bozeman MT ----- Original Message ----- From: "Kinsley, David" To: Sent: Tuesday, January 08, 2008 4:50 PM Subject: [Histonet] CD11c on paraffin? Hi, Does anyone know of a CD11c antibody that works on FFPE tissue sections? I'm looking for antibodies that work on either human or mouse tissues. My samples are fixed in formalin for 24 hours then transferred to 70% ETOH and processed into paraffin. Thanks in advance Dave ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tim.morken <@t> thermofisher.com Wed Jan 9 10:05:19 2008 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Wed Jan 9 10:06:05 2008 Subject: [Histonet] Biocare p16 In-Reply-To: <00e301c85292$8ff74930$0302a8c0@yourxhtr8hvc4p> References: <8E7AD740937B954F947F0DB4467EFEE056EA09@mail.srhs-pa.org><000601c8526b$30c08200$6401a8c0@your8ba846406f> <00e301c85292$8ff74930$0302a8c0@yourxhtr8hvc4p> Message-ID: <6BFF6D137DF6BC43B33891BA96E83B19010DA498@PGHCR-EXMB-VS-1.na.fshrnet.com> It's true, MTM labs holds the patent to any possible antibody made for p16 protein. They are marketing it themselves. So, it is not just Biocare and Cell Marque, but many companies will not carry p16 anymore (depends on whether it is cost effective to pay the licensing fees and royalties demanded). Tim Morken Lab Vision Products Anatomical Pathology ThermoFisher Scientific -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Tuesday, January 08, 2008 11:38 PM To: Cathy; 'Jones, Laura'; 'Histonet (E-mail)' Subject: Re: [Histonet] Biocare p16 Flame time See, this is what just fry my cookies about some of these vendors. Tell me this isn't a money issue. What happened to patient care, or is that obsolete now? I hate it when some one has a good product, then screws it up by getting greedy about it. Now, you have to purchase the detection kit to get the antibody. Bull do-do. Now I'm really urinated off. Get me the CEO. As some of you know, I have no qualms about getting into the face of a CEO. I had plenty of experience at my last job, not to mention a couple of NSH meetings too. JTT ----- Original Message ----- From: "Cathy" To: "'Jones, Laura'" ; "'Histonet (E-mail)'" Sent: Tuesday, January 08, 2008 8:56 PM Subject: RE: [Histonet] Biocare p16 > > We were notified last October by Cell Marque that MTM Labs had decided to > market p16 themselves. I have spoken with their rep here in North America > and was told that we would have to purchase the entire kit but would be > able > to use only the antibody (we have Ventana Benchmarks). The cost per test > will double but our pathologists have decided that we still need to have > p16 > available. The rest of the kit will end up being discarded. > > Cathy > Kelowna, B.C. > Canada > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jones, > Laura > Sent: Monday, January 07, 2008 12:39 PM > To: Histonet (E-mail) > Subject: [Histonet] Biocare p16 > > We are just wondering if anyone else has been affected by Biocare's > inability to sell p16 and what you all have done to replace it? We were > told by Biocare that we'd have to order the antibody (and the entire > detection kit, which we don't want) from a company in Germany - MTM Labs. > My Pathologist would appreciate your advice. Thanks in advance! Laura > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.5.516 / Virus Database: 269.17.13/1211 - Release Date: 1/6/2008 > 11:57 AM > > > No virus found in this outgoing message. > Checked by AVG Free Edition. > Version: 7.5.516 / Virus Database: 269.17.13/1214 - Release Date: 1/8/2008 > 1:38 PM > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sharon.Davis-Devine <@t> carle.com Wed Jan 9 10:19:06 2008 From: Sharon.Davis-Devine <@t> carle.com (Sharon.Davis-Devine) Date: Wed Jan 9 10:19:30 2008 Subject: [Histonet] Who can perform which job functions Message-ID: <44780C571F28624DBB446DE55C4D733A021E083A@EXCHANGEBE1.carle.com> Ok, all of you Histonetters I have another question for you. Who the histology laboratory can perform these job functions: embedding, cutting, performing special stains and IHC? Can a lab assistant perform these duties if properly trained or do you have to be classified as a Histotech in training? Can a Cytotech or Med Tech perform such duties, again if properly trained? All opinions and references to such requirements would be greatly appreciated. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com From Rcartun <@t> harthosp.org Wed Jan 9 10:20:16 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Jan 9 10:20:52 2008 Subject: [Histonet] CD11c on paraffin? In-Reply-To: References: Message-ID: <4784ADF0020000770000A106@gwmail4.harthosp.org> Leica Microsystems (formerly Vision Biosystems). Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Kinsley, David" 01/08/08 6:50 PM >>> Hi, Does anyone know of a CD11c antibody that works on FFPE tissue sections? I'm looking for antibodies that work on either human or mouse tissues. My samples are fixed in formalin for 24 hours then transferred to 70% ETOH and processed into paraffin. Thanks in advance Dave ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From Ngale <@t> bccancer.bc.ca Wed Jan 9 10:35:23 2008 From: Ngale <@t> bccancer.bc.ca (Gale, Nadia) Date: Wed Jan 9 10:35:48 2008 Subject: [Histonet] IGF1-Receptor Message-ID: I posted a message a few days ago and, to my surprise, have received no responses. Is noone using an antibody to human IGF1-Receptor on ffpe? Any info whatsoever, even if you've tried something without success, would be greatly appreciated :) Thanks! Nadia Nadia Gale Lead Histotechnologist Centre for Translational and Applied Genomics (CTAG) 3427-600 West 10th Avenue Vancouver, BC V5Z 4E6 tel 604-877-6000 ext 2426 fax 604-877-6089 ngale@bccancer.bc.ca From cmiller <@t> physlab.com Wed Jan 9 10:35:17 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Wed Jan 9 10:36:01 2008 Subject: [Histonet] Response to On-Call pay In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF0222F446@wahtntex2.waht.swest.nhs.uk> References: <86ADE4EB583CE64799A9924684A0FBBF0222F446@wahtntex2.waht.swest.nhs.uk> Message-ID: <007101c852dd$9e7b9b50$3d02a8c0@plab.local> Ridiculous, Re read her message. Who would actually work for 3 dollars an hour?? If she gets called back in she will earn (using a wage of $25 dollars an hour) a minimum of 49 dollars an hour. Not including the shift differential pay nor the $3 dollars an hour she continues to earn the rest of the weekend sitting at home (approx $ 144 dollars the rest of the weekend) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: Wednesday, January 09, 2008 8:07 AM To: MICHELLE MCNEESE; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Response to On-Call pay $3 per hour???? That's about 50p at current exchange rates; I really don't know how you Americans can afford to live. I mean a nice bottle of Chardonnay or Chablis must be a weeks wages. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Don't be afraid to take a big step when one is indicated. You can't cross a chasm in two small jumps. --Buckminster Fuller This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From Jerry <@t> ralambusa.com Wed Jan 9 11:40:22 2008 From: Jerry <@t> ralambusa.com (Jerry Helisek) Date: Wed Jan 9 11:40:47 2008 Subject: [Histonet] Surgical Pathology Tracking System Message-ID: <3855F92002259948A66A8CA2D16E3A4F05A768@server.ralambusa.com> Patty, Please see: http://www.ralamb.net/product_info.php?products_id=599&osCsid=88c456fca1 a01f8e6f134ebe92390756 The LambTrack system provides full tracking and traceability of items and any items that you choose to link to one another, via digital signature/trace. For example a req form can be linked to a tissue sample that can be linked to several cassettes and then too slides as well as who "touched" the items on down the line to storage and retrieval. LambTrack can also be used for inventory, productivity, verification and more!! Because its scaleable you only chose the "modules"/functions you need. ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ Message: 22 Date: Tue, 8 Jan 2008 22:15:39 EST From: Histopatty@aol.com Subject: [Histonet] Histology Tracking System To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Are there any tracking systems for Surgical pathology out there? I am aware of two, one is private and the other is in the making. We have meditech. Patty Eneff OUMC 405-271-5653 ------------------------------ From Sharon.Davis-Devine <@t> carle.com Wed Jan 9 13:08:25 2008 From: Sharon.Davis-Devine <@t> carle.com (Sharon.Davis-Devine) Date: Wed Jan 9 13:08:48 2008 Subject: [Histonet] H & E stain question Message-ID: <44780C571F28624DBB446DE55C4D733A021E083B@EXCHANGEBE1.carle.com> We are continually having a problem with our H & E stain. We currently use Gill III Hematoxlyin on a staining machine with a running water bath. Our pathologists do not like to have the pH of the Heme higher than 2.5 because it makes the small biopsy sections too dark and the mucin appears blue. My question to all of you is, has any of you experienced this issue and if so, how did you go about getting it fixed? I had suggested switching to a different heme such as Harris and maybe switching our running water bath to DI water. We have significant flooding in our area and there will be more bleach in the city water supply. Does any of this sound feasible? Help please. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com From anthony <@t> histotechexchange.com Wed Jan 9 13:21:17 2008 From: anthony <@t> histotechexchange.com (anthony@histotechexchange.com) Date: Wed Jan 9 13:28:36 2008 Subject: [Histonet] Who can perform which job functions In-Reply-To: <44780C571F28624DBB446DE55C4D733A021E083A@EXCHANGEBE1.carle.com> References: <44780C571F28624DBB446DE55C4D733A021E083A@EXCHANGEBE1.carle.com> Message-ID: <1483.65.40.219.156.1199906477.squirrel@host7.wfdns.com> Dear Sharon: This is as far as I am concerned very difficult to answer. This is because you have to bring in High Complexity Testing for the IHC. This is covered by CLIA 88 and that goes something like this. NOTE: The laboratory director may delegate the dissection of specimens to non-pathologist individuals; these individuals must be qualified as high complexity testing personnel under CLIA-88 regulations. The minimum training/experience required of such personnel is: 1. An earned associate degree in a laboratory science or medical laboratory technology, obtained from an accredited institution, OR 2. Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education must include 24 semester hours of medical laboratory technology courses, OR 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. In addition, the individual must have laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, the CAHEA, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above),OR at least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. In addition, the CLIA-88 regulations include exceptions for grandfathered individuals; these regulations (42CFR493.1489 and 1491) may be found at http://www.phppo.cdc.gov/clia/regs/subpart_m.aspx#493.1487 It is the responsibility of the laboratory director to determine whether an individual's education, training and experience satisfies the requirements of this checklist question. This checklist question applies only to laboratories subject to CLIA-88. And if you see at the end if you are not subject to CLIA-88 then you do not need to worry about it. Question is, are you subject to CLIA-88 under CAP regulations. What is the mandate for CLIA-88: Subpart A--General Provisions Source: 57 FR 7139, Feb. 28, 1992, unless otherwise noted. Sec. 493.1 Basis and scope. This part sets forth the conditions that all laboratories must meet to be certified to perform testing on human specimens under the Clinical Laboratory Improvement Amendments of 1988 (CLIA). It implements sections 1861 (e) and (j), the sentence following section 1861(s)(13), and 1902(a)(9) of the Social Security Act, and section 353 of the Public Health Service Act. This part applies to all laboratories as defined under ``laboratory'' in Sec. 493.2 of this part. This part also applies to laboratories seeking payment under the Medicare and Medicaid programs. The requirements are the same for Medicare approval as for CLIA certification. So as I understand it if you do not have an AA with 24 hours of a combination of Chemistry and Biology or were grandfathered in with a HTL (Not a HT), you should not be grossing or doing Immunos even if you are a certified HT. I apologize if I am wrong and please set me right. If someone out there can send me a flow diagram of how all the certifications is intertwined, what is mandated and what is organization based (i.e. ASCP) I would LOVE to get it. Yours truly, Anthony Williams BSc. HT (ASCP) Histotech Exchange LLC 19 Whitmore Street Lexington, VA 24450 T 1 877 464 8911 F 1 540 301 0071 anthony@histotechexchange.com www.histotechexchange.com Ok, all of you Histonetters I have another question for you. Who the > histology laboratory can perform these job functions: embedding, > cutting, performing special stains and IHC? Can a lab assistant perform > these duties if properly trained or do you have to be classified as a > Histotech in training? Can a Cytotech or Med Tech perform such duties, > again if properly trained? All opinions and references to such > requirements would be greatly appreciated. > > > > Sharon Davis-Devine, CT (ASCP) > > Cytology Supervisor > > Carle Clinic > > 602 West University > > Urbana, Illinois 61801 > > Phone: 217-383-3572 > > Email: sharon.davis-devine@carle.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Jerry <@t> ralambusa.com Wed Jan 9 13:41:29 2008 From: Jerry <@t> ralambusa.com (Jerry Helisek) Date: Wed Jan 9 13:41:54 2008 Subject: [Histonet] NG Staining Protocol Message-ID: <3855F92002259948A66A8CA2D16E3A4F05A777@server.ralambusa.com> I am looking for some NG staining protocols, I was looking for some guidance... Thanks! ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ From liz <@t> premierlab.com Wed Jan 9 14:16:34 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Jan 9 14:16:58 2008 Subject: [Histonet] fibrin or fibrinogen antibody thats specific to human only Message-ID: Hello All I hope everyone had a great holiday and a happy new year. I was wondering if anyone out there knows of antibodies to either human fibrin or fibrinogen that will not cross react with porcine or visa versa. I need to detect one or the other in FFPE samples. I have the dako antibody, but that cross reacts with porcine. I have seen online a fibrin antibody with a clone of 59D8 that works on human but not porcine tissue, but I can not seem to find a source. Any help would be appreciated, and thanks in advance. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 From NMargaryan <@t> childrensmemorial.org Wed Jan 9 14:19:45 2008 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Wed Jan 9 14:20:11 2008 Subject: [Histonet] Tuj 1 Message-ID: Dear friends, I need a protocol for Tuj 1 staining on tissue. Any protocol will be great. Thanks in advance, Naira From llewllew <@t> shaw.ca Wed Jan 9 15:47:35 2008 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Wed Jan 9 15:49:29 2008 Subject: [Histonet] H&E question Message-ID: <000701c85309$3f132710$05024246@yourlk4rlmsu> Chances are the biopsies are too dark and mucin is staining because you are using Gill III. This is a very strong hemalum with 6 grams hematoxylin per litre. If all is oxidised I would be surprised if mucin did not stain. Strong hemalums which are fully oxidised nearly always stain acid mucins, especially if they are becoming acid deficient. Try switching to Gill II or another hemalum with less hematoxylin, or dilute your Gill III by half or by a third with a solution made up the same as the Gill III, but without the hematoxylin and oxidiser. Try Cole's progressive hemalum, or some other type. Cut a lot of sections and carry out some experiments on typical types of tissues you get until the staining is satisfactory. Go to http://stainsfile.info and read the articles there about formulating hemalum and the other articles about hematoxylin staining. Rather than use deionised water, store tap water over some marble chips in a 20 gallon container and use that for the final rinses after blueing the hemalum and before the eosin. Calcium ions are reported to improve eosin staining. They also would make the water alkaline and ensure the blueing is retained. Bryan Llewellyn ----- Original Message ----- From: "Sharon.Davis-Devine" To: Sent: Wednesday, January 09, 2008 11:08 AM Subject: [Histonet] H & E stain question We are continually having a problem with our H & E stain. We currently use Gill III Hematoxlyin on a staining machine with a running water bath. Our pathologists do not like to have the pH of the Heme higher than 2.5 because it makes the small biopsy sections too dark and the mucin appears blue. My question to all of you is, has any of you experienced this issue and if so, how did you go about getting it fixed? I had suggested switching to a different heme such as Harris and maybe switching our running water bath to DI water. We have significant flooding in our area and there will be more bleach in the city water supply. Does any of this sound feasible? Help please. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Wed Jan 9 15:59:39 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Wed Jan 9 15:59:57 2008 Subject: [Histonet] H & E stain question References: <44780C571F28624DBB446DE55C4D733A021E083B@EXCHANGEBE1.carle.com> Message-ID: <001401c8530a$ee874540$6501a8c0@DHXTS541> Dear Sharon, Use a different Gill hematoxylin. We used Gill 2 for all our FFPE embedded tissues for years, a preference over Gill 3 which is more concentrated than Gill 2. You could also try Gill 1 which is less concentrated than 2 or 3. I suggest a test run of several sections with ech section stained at a different time to optimize staining time for these OR cut back on the staining time in Gill 3 - you did not give the time? Most progressive hematoxylins will have stained well at around 1 1/2 to 2 minutes anyway. The only time we ever used Gill 3 was for glycol methacrylate embedded tissues as we found it too concentrated for FFPE tissues in our lab. Gayle M. Callis HT/HTL/MT(ASCP) Bozeman MT ----- Original Message ----- From: "Sharon.Davis-Devine" To: Sent: Wednesday, January 09, 2008 12:08 PM Subject: [Histonet] H & E stain question We are continually having a problem with our H & E stain. We currently use Gill III Hematoxlyin on a staining machine with a running water bath. Our pathologists do not like to have the pH of the Heme higher than 2.5 because it makes the small biopsy sections too dark and the mucin appears blue. My question to all of you is, has any of you experienced this issue and if so, how did you go about getting it fixed? I had suggested switching to a different heme such as Harris and maybe switching our running water bath to DI water. We have significant flooding in our area and there will be more bleach in the city water supply. Does any of this sound feasible? Help please. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Wed Jan 9 17:32:28 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Jan 9 17:33:08 2008 Subject: [Histonet] renal tubules Message-ID: <000a01c85317$ed4c6860$6501a8c0@Patsyoffice> Folks, I have an investigator that wants to demonstrate damage to renal tubules in rats caused by giving them uranyl acetate. Can you recommend special stains for that? Would it be something like PAS? Also are there antibodies we could use on ffpe rat kidneys that might help with this project? Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From anthony <@t> histotechexchange.com Wed Jan 9 18:58:50 2008 From: anthony <@t> histotechexchange.com (anthony@histotechexchange.com) Date: Wed Jan 9 19:06:21 2008 Subject: [Histonet] Who can perform which job functions In-Reply-To: <000601c85302$5b21f050$3601a8c0@brownpathology.net> References: <000601c85302$5b21f050$3601a8c0@brownpathology.net> Message-ID: <2199.65.40.219.156.1199926730.squirrel@host7.wfdns.com> Dear Bonnie: Please explain about AP and if CLIA 88 regards IHC as high complexity testing is there a conflict with AP or are you saying that IHC was never considered high complexity testing? I only ask because when I went to the nationals in Toronto there was a workshop on IHC and there was some discussion about it being High Complexity Testing. In the best tradition of Gunther I may be wrong. Yours truly, Anthony Williams BSc. HT (ASCP) Histotech Exchange LLC 19 Whitmore Street Lexington, VA 24450 T 1 877 464 8911 F 1 540 301 0071 anthony@histotechexchange.com www.histotechexchange.com Grossing yes-- IHC no, unless they slipped something in very recently, it > is > still covered under the 'pathologists as the testing personnel' clause in > AP. > > Bonnie Whitaker > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > anthony@histotechexchange.com > Sent: Wednesday, January 09, 2008 1:21 PM > To: Sharon.Davis-Devine > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Who can perform which job functions > > > Dear Sharon: > > This is as far as I am concerned very difficult to answer. This is because > you have to bring in High Complexity Testing for the IHC. This is covered > by > CLIA 88 and that goes something like this. > > NOTE: The laboratory director may delegate the dissection of specimens to > non-pathologist individuals; these individuals must be qualified as high > complexity testing personnel under CLIA-88 regulations. The minimum > training/experience required of such personnel is: > > 1. An earned associate degree in a laboratory science or medical > laboratory > technology, obtained from an accredited institution, OR 2. > Education/training equivalent to the above that includes at least 60 > semester hours or equivalent from an accredited institution. This > education > must include 24 semester hours of medical laboratory technology courses, > OR > 24 semester hours of science courses that includes 6 semester hours of > chemistry, 6 semester hours of biology, and 12 semester hours of > chemistry, > biology or medical laboratory technology in any combination. > In addition, the individual must have laboratory training including either > completion of a clinical laboratory training program approved or > accredited > by the ABHES, the CAHEA, or other organization approved by HHS (note that > this training may be included in the 60 semester hours listed above),OR at > least 3 months documented laboratory training in each specialty in which > the > individual performs high complexity testing. > > In addition, the CLIA-88 regulations include exceptions for > grandfathered individuals; these regulations (42CFR493.1489 and 1491) > may > be found at > http://www.phppo.cdc.gov/clia/regs/subpart_m.aspx#493.1487 > > It is the responsibility of the laboratory director to determine whether > an > individual's education, training and experience satisfies the requirements > of this checklist question. > > This checklist question applies only to laboratories subject to CLIA-88. > > And if you see at the end if you are not subject to CLIA-88 then you do > not > need to worry about it. Question is, are you subject to CLIA-88 under CAP > regulations. What is the mandate for CLIA-88: > > Subpart A--General Provisions > > Source: 57 FR 7139, Feb. 28, 1992, unless otherwise noted. > > Sec. 493.1 Basis and scope. > > This part sets forth the conditions that all laboratories must meet to be > certified to perform testing on human specimens under the Clinical > Laboratory Improvement Amendments of 1988 (CLIA). It implements sections > 1861 (e) and (j), the sentence following section 1861(s)(13), and > 1902(a)(9) of the Social Security Act, and section 353 of the Public > Health > Service Act. This part applies to all laboratories as defined under > ``laboratory'' in Sec. 493.2 of this part. This part also applies to > laboratories seeking payment under the Medicare and Medicaid programs. The > requirements are the same for Medicare approval as for CLIA certification. > > So as I understand it if you do not have an AA with 24 hours of a > combination of Chemistry and Biology or were grandfathered in with a HTL > (Not a HT), you should not be grossing or doing Immunos even if you are a > certified HT. > > I apologize if I am wrong and please set me right. > > If someone out there can send me a flow diagram of how all the > certifications is intertwined, what is mandated and what is organization > based (i.e. ASCP) I would LOVE to get it. > > Yours truly, > > Anthony Williams BSc. HT (ASCP) > Histotech Exchange LLC > 19 Whitmore Street > Lexington, VA 24450 > T 1 877 464 8911 > F 1 540 301 0071 > anthony@histotechexchange.com > www.histotechexchange.com > > > Ok, all of you Histonetters I have another question for you. Who the >> histology laboratory can perform these job functions: embedding, >> cutting, performing special stains and IHC? Can a lab assistant >> perform these duties if properly trained or do you have to be >> classified as a Histotech in training? Can a Cytotech or Med Tech >> perform such duties, again if properly trained? All opinions and >> references to such requirements would be greatly appreciated. >> >> >> >> Sharon Davis-Devine, CT (ASCP) >> >> Cytology Supervisor >> >> Carle Clinic >> >> 602 West University >> >> Urbana, Illinois 61801 >> >> Phone: 217-383-3572 >> >> Email: sharon.davis-devine@carle.com >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From anthony <@t> histotechexchange.com Wed Jan 9 19:11:21 2008 From: anthony <@t> histotechexchange.com (anthony@histotechexchange.com) Date: Wed Jan 9 19:18:43 2008 Subject: [Histonet] Who can perform which job functions In-Reply-To: <000701c85303$30846750$3601a8c0@brownpathology.net> References: <000701c85303$30846750$3601a8c0@brownpathology.net> Message-ID: <2345.65.40.219.156.1199927481.squirrel@host7.wfdns.com> Dear Bonnie: I would just ask; is your position substantiated by the language of the statute? I refers to a Technologist(HTL) not a Technition (HT). It will help me if I am wrong by the way, thanks for the help. (a) Possess a current license as a laboratory technologist issued by the State, if such licensing exists; and (b)(1) Have earned a bachelor's degree in medical technology from an accredited university; or (2) Have successfully completed 3 years of academic study (a minimum of 90 semester hours or equivalent) in an accredited college or university, which met the specific requirements for entrance into a school of medical technology accredited by an accrediting agency approved by the Secretary, and has successfully completed a course of training of at least 12 months in such a school; or (3) Have earned a bachelor's degree in one of the chemical, physical, or biological sciences and, in addition, has at least 1 year of pertinent full-time laboratory experience or training, or both, in the specialty or subspecialty in which the individual performs tests; or (4)(i) Have successfully completed 3 years (90 semester hours or equivalent) in an accredited college or university with the following distribution of courses-- (A) For those whose training was completed before September 15, 1963. At least 24 semester hours in chemistry and biology courses of which-- (1) At least 6 semester hours were in inorganic chemistry and at least 3 semester hours were in other chemistry courses; and (2) At least 12 semester hours in biology courses pertinent to the medical sciences; or (B) For those whose training was completed after September 14, 1963. (1) 16 semester hours in chemistry courses that included at least 6 semester hours in inorganic chemistry and that are acceptable toward a major in chemistry; (2) 16 semester hours in biology courses that are pertinent to the medical sciences and are acceptable toward a major in the biological sciences; and (3) 3 semester hours of mathematics; and (ii) Has experience, training, or both, covering several fields of medical laboratory work of at least 1 year and of such quality as to provide him or her with education and training in medical technology equivalent to that described in paragraphs (b)(1) and (2) of this section; or (5) With respect to individuals first qualifying before July 1, 1971, the technologist-- (i) Was performing the duties of a laboratory technologist at any time between July 1, 1961, and January 1, 1968, and (ii) Has had at least 10 years of pertinent laboratory experience prior to January 1, 1968. (This required experience may be met by substitution of education for experience); or (6) Achieves a satisfactory grade in a proficiency examination approved by HHS. Anthony Williams BSc. HT (ASCP) Histotech Exchange LLC 19 Whitmore Street Lexington, VA 24450 T 1 877 464 8911 F 1 540 301 0071 anthony@histotechexchange.com www.histotechexchange.com Hi Anthony, > > Grandfathering in to gross only means that you were grossing before that > cutoff date. it matters not one bit if you have any certification as far > as > CLIA is concerned. That is how it was explained to me by an ex-CLIA > inspector. > > Bonnie Whitaker > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > anthony@histotechexchange.com > Sent: Wednesday, January 09, 2008 1:21 PM > To: Sharon.Davis-Devine > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Who can perform which job functions > > > Dear Sharon: > > This is as far as I am concerned very difficult to answer. This is because > you have to bring in High Complexity Testing for the IHC. This is covered > by > CLIA 88 and that goes something like this. > > NOTE: The laboratory director may delegate the dissection of specimens to > non-pathologist individuals; these individuals must be qualified as high > complexity testing personnel under CLIA-88 regulations. The minimum > training/experience required of such personnel is: > > 1. An earned associate degree in a laboratory science or medical > laboratory > technology, obtained from an accredited institution, OR 2. > Education/training equivalent to the above that includes at least 60 > semester hours or equivalent from an accredited institution. This > education > must include 24 semester hours of medical laboratory technology courses, > OR > 24 semester hours of science courses that includes 6 semester hours of > chemistry, 6 semester hours of biology, and 12 semester hours of > chemistry, > biology or medical laboratory technology in any combination. > In addition, the individual must have laboratory training including either > completion of a clinical laboratory training program approved or > accredited > by the ABHES, the CAHEA, or other organization approved by HHS (note that > this training may be included in the 60 semester hours listed above),OR at > least 3 months documented laboratory training in each specialty in which > the > individual performs high complexity testing. > > In addition, the CLIA-88 regulations include exceptions for > grandfathered individuals; these regulations (42CFR493.1489 and 1491) > may > be found at > http://www.phppo.cdc.gov/clia/regs/subpart_m.aspx#493.1487 > > It is the responsibility of the laboratory director to determine whether > an > individual's education, training and experience satisfies the requirements > of this checklist question. > > This checklist question applies only to laboratories subject to CLIA-88. > > And if you see at the end if you are not subject to CLIA-88 then you do > not > need to worry about it. Question is, are you subject to CLIA-88 under CAP > regulations. What is the mandate for CLIA-88: > > Subpart A--General Provisions > > Source: 57 FR 7139, Feb. 28, 1992, unless otherwise noted. > > Sec. 493.1 Basis and scope. > > This part sets forth the conditions that all laboratories must meet to be > certified to perform testing on human specimens under the Clinical > Laboratory Improvement Amendments of 1988 (CLIA). It implements sections > 1861 (e) and (j), the sentence following section 1861(s)(13), and > 1902(a)(9) of the Social Security Act, and section 353 of the Public > Health > Service Act. This part applies to all laboratories as defined under > ``laboratory'' in Sec. 493.2 of this part. This part also applies to > laboratories seeking payment under the Medicare and Medicaid programs. The > requirements are the same for Medicare approval as for CLIA certification. > > So as I understand it if you do not have an AA with 24 hours of a > combination of Chemistry and Biology or were grandfathered in with a HTL > (Not a HT), you should not be grossing or doing Immunos even if you are a > certified HT. > > I apologize if I am wrong and please set me right. > > If someone out there can send me a flow diagram of how all the > certifications is intertwined, what is mandated and what is organization > based (i.e. ASCP) I would LOVE to get it. > > Yours truly, > > Anthony Williams BSc. HT (ASCP) > Histotech Exchange LLC > 19 Whitmore Street > Lexington, VA 24450 > T 1 877 464 8911 > F 1 540 301 0071 > anthony@histotechexchange.com > www.histotechexchange.com > > > Ok, all of you Histonetters I have another question for you. Who the >> histology laboratory can perform these job functions: embedding, >> cutting, performing special stains and IHC? Can a lab assistant >> perform these duties if properly trained or do you have to be >> classified as a Histotech in training? Can a Cytotech or Med Tech >> perform such duties, again if properly trained? All opinions and >> references to such requirements would be greatly appreciated. >> >> >> >> Sharon Davis-Devine, CT (ASCP) >> >> Cytology Supervisor >> >> Carle Clinic >> >> 602 West University >> >> Urbana, Illinois 61801 >> >> Phone: 217-383-3572 >> >> Email: sharon.davis-devine@carle.com >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From godsgalnow <@t> aol.com Wed Jan 9 20:26:11 2008 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Wed Jan 9 20:27:19 2008 Subject: [Histonet] Who can perform which job functions In-Reply-To: <2345.65.40.219.156.1199927481.squirrel@host7.wfdns.com> References: <000701c85303$30846750$3601a8c0@brownpathology.net> <2345.65.40.219.156.1199927481.squirrel@host7.wfdns.com> Message-ID: <8CA21474CCF3E0B-FC4-1DA7@mblk-d16.sysops.aol.com> IHC, grossing, Flow, FISH,?etc...are considered high complexity testing.? Embedding, microtomy, special stains.....these are all considered moderate complexity testing. Roxanne Soto HT(ASCP)QIHC -----Original Message----- From: anthony@histotechexchange.com To: Bonnie Whitaker Cc: histonet@lists.utsouthwestern.edu Sent: Wed, 9 Jan 2008 8:11 pm Subject: RE: [Histonet] Who can perform which job functions Dear Bonnie: I would just ask; is your position substantiated by the language of the statute? I refers to a Technologist(HTL) not a Technition (HT). It will help me if I am wrong by the way, thanks for the help. (a) Possess a current license as a laboratory technologist issued by the State, if such licensing exists; and (b)(1) Have earned a bachelor's degree in medical technology from an accredited university; or (2) Have successfully completed 3 years of academic study (a minimum of 90 semester hours or equivalent) in an accredited college or university, which met the specific requirements for entrance into a school of medical technology accredited by an accrediting agency approved by the Secretary, and has successfully completed a course of training of at least 12 months in such a school; or (3) Have earned a bachelor's degree in one of the chemical, physical, or biological sciences and, in addition, has at least 1 year of pertinent full-time laboratory experience or training, or both, in the specialty or subspecialty in which the individual performs tests; or (4)(i) Have successfully completed 3 years (90 semester hours or equivalent) in an accredited college or university with the following distribution of courses-- (A) For those whose training was completed before September 15, 1963. At least 24 semester hours in chemistry and biology courses of which-- (1) At least 6 semester hours were in inorganic chemistry and at least 3 semester hours were in other chemistry courses; and (2) At least 12 semester hours in biology courses pertinent to the medical sciences; or (B) For those whose training was completed after September 14, 1963. (1) 16 semester hours in chemistry courses that included at least 6 semester hours in inorganic chemistry and that are acceptable toward a major in chemistry; (2) 16 semester hours in biology courses that are pertinent to the medical sciences and are acceptable toward a major in the biological sciences; and (3) 3 semester hours of mathematics; and (ii) Has experience, training, or both, covering several fields of medical laboratory work of at least 1 year and of such quality as to provide him or her with education and training in medical technology equivalent to that described in paragraphs (b)(1) and (2) of this section; or (5) With respect to individuals first qualifying before July 1, 1971, the technologist-- (i) Was performing the duties of a laboratory technologist at any time between July 1, 1961, and January 1, 1968, and (ii) Has had at least 10 years of pertinent laboratory experience prior to January 1, 1968. (This required experience may be met by substitution of education for experience); or (6) Achieves a satisfactory grade in a proficiency examination approved by HHS. Anthony Williams BSc. HT (ASCP) Histotech Exchange LLC 19 Whitmore Street Lexington, VA 24450 T 1 877 464 8911 F 1 540 301 0071 anthony@histotechexchange.com www.histotechexchange.com Hi Anthony, > > Grandfathering in to gross only means that you were grossing before that > cutoff date. it matters not one bit if you have any certification as far > as > CLIA is concerned. That is how it was explained to me by an ex-CLIA > inspector. > > Bonnie Whitaker > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > anthony@histotechexchange.com > Sent: Wednesday, January 09, 2008 1:21 PM > To: Sharon.Davis-Devine > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Who can perform which job functions > > > Dear Sharon: > > This is as far as I am concerned very difficult to answer. This is because > you have to bring in High Complexity Testing for the IHC. This is covered > by > CLIA 88 and that goes something like this. > > NOTE: The laboratory director may delegate the dissection of specimens to > non-pathologist individuals; these individuals must be qualified as high > complexity testing personnel under CLIA-88 regulations. The minimum > training/experience required of such personnel is: > > 1. An earned associate degree in a laboratory science or medical > laboratory > technology, obtained from an accredited institution, OR 2. > Education/training equivalent to the above that includes at least 60 > semester hours or equivalent from an accredited institution. This > education > must include 24 semester hours of medical laboratory technology courses, > OR > 24 semester hours of science courses that includes 6 semester hours of > chemistry, 6 semester hours of biology, and 12 semester hours of > chemistry, > biology or medical laboratory technology in any combination. > In addition, the individual must have laboratory training including either > completion of a clinical laboratory training program approved or > accredited > by the ABHES, the CAHEA, or other organization approved by HHS (note that > this training may be included in the 60 semester hours listed above),OR at > least 3 months documented laboratory training in each specialty in which > the > individual performs high complexity testing. > > In addition, the CLIA-88 regulations include exceptions for > grandfathered individuals; these regulations (42CFR493.1489 and 1491) > may > be found at > http://www.phppo.cdc.gov/clia/regs/subpart_m.aspx#493.1487 > > It is the responsibility of the laboratory director to determine whether > an > individual's education, training and experience satisfies the requirements > of this checklist question. > > This checklist question applies only to laboratories subject to CLIA-88. > > And if you see at the end if you are not subject to CLIA-88 then you do > not > need to worry about it. Question is, are you subject to CLIA-88 under CAP > regulations. What is the mandate for CLIA-88: > > Subpart A--General Provisions > > Source: 57 FR 7139, Feb. 28, 1992, unless otherwise noted. > > Sec. 493.1 Basis and scope. > > This part sets forth the conditions that all laboratories must meet to be > certified to perform testing on human specimens under the Clinical > Laboratory Improvement Amendments of 1988 (CLIA). It implements sections > 1861 (e) and (j), the sentence following section 1861(s)(13), and > 1902(a)(9) of the Social Security Act, and section 353 of the Public > Health > Service Act. This part applies to all laboratories as defined under > ``laboratory'' in Sec. 493.2 of this part. This part also applies to > laboratories seeking payment under the Medicare and Medicaid programs. The > requirements are the same for Medicare approval as for CLIA certification. > > So as I understand it if you do not have an AA with 24 hours of a > combination of Chemistry and Biology or were grandfathered in with a HTL > (Not a HT), you should not be grossing or doing Immunos even if you are a > certified HT. > > I apologize if I am wrong and please set me right. > > If someone out there can send me a flow diagram of how all the > certifications is intertwined, what is mandated and what is organization > based (i.e. ASCP) I would LOVE to get it. > > Yours truly, > > Anthony Williams BSc. HT (ASCP) > Histotech Exchange LLC > 19 Whitmore Street > Lexington, VA 24450 > T 1 877 464 8911 > F 1 540 301 0071 > anthony@histotechexchange.com > www.histotechexchange.com > > > Ok, all of you Histonetters I have another question for you. Who the >> histology laboratory can perform these job functions: embedding, >> cutting, performing special stains and IHC? Can a lab assistant >> perform these duties if properly trained or do you have to be >> classified as a Histotech in training? Can a Cytotech or Med Tech >> perform such duties, again if properly trained? All opinions and >> references to such requirements would be greatly appreciated. >> >> >> >> Sharon Davis-Devine, CT (ASCP) >> >> Cytology Supervisor >> >> Carle Clinic >> >> 602 West University >> >> Urbana, Illinois 61801 >> >> Phone: 217-383-3572 >> >> Email: sharon.davis-devine@carle.com >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ More new features than ever. Check out the new AOL Mail ! - http://webmail.aol.com From anthony <@t> histotechexchange.com Wed Jan 9 21:12:57 2008 From: anthony <@t> histotechexchange.com (anthony@histotechexchange.com) Date: Wed Jan 9 21:20:20 2008 Subject: [Histonet] Who can perform which job functions In-Reply-To: <8CA21474CCF3E0B-FC4-1DA7@mblk-d16.sysops.aol.com> References: <000701c85303$30846750$3601a8c0@brownpathology.net> <2345.65.40.219.156.1199927481.squirrel@host7.wfdns.com> <8CA21474CCF3E0B-FC4-1DA7@mblk-d16.sysops.aol.com> Message-ID: <2568.65.40.219.156.1199934777.squirrel@host7.wfdns.com> Dear Roxanne: So to clarify: You are saying that these are considered high complexity testing. "IHC, grossing, Flow, FISH,?etc...are considered high complexity testing.?" Or are you asking if they are? Yours truly, Anthony Williams BSc. HT (ASCP) > Histotech Exchange LLC > 19 Whitmore Street > Lexington, VA 24450 > T 1 877 464 8911 > F 1 540 301 0071 > anthony@histotechexchange.com > www.histotechexchange.com > Embedding, microtomy, special stains.....these are all considered moderate > complexity testing. > > > > > > Roxanne Soto HT(ASCP)QIHC > > -----Original Message----- > From: anthony@histotechexchange.com > To: Bonnie Whitaker > Cc: histonet@lists.utsouthwestern.edu > Sent: Wed, 9 Jan 2008 8:11 pm > Subject: RE: [Histonet] Who can perform which job functions > > > > Dear Bonnie: > > I would just ask; is your position substantiated by the language of the > statute? > > I refers to a Technologist(HTL) not a Technition (HT). > > It will help me if I am wrong by the way, thanks for the help. > > (a) Possess a current license as a laboratory technologist issued by > the State, if such licensing exists; and > (b)(1) Have earned a bachelor's degree in medical technology from an > accredited university; or > (2) Have successfully completed 3 years of academic study (a minimum > of 90 semester hours or equivalent) in an accredited college or > university, which met the specific requirements for entrance into a > school of medical technology accredited by an accrediting agency > approved by the Secretary, and has successfully completed a course > of > training of at least 12 months in such a school; or > (3) Have earned a bachelor's degree in one of the chemical, > physical, or biological sciences and, in addition, has at least 1 > year > of pertinent full-time laboratory experience or training, or both, > in > the specialty or subspecialty in which the individual performs > tests; or > (4)(i) Have successfully completed 3 years (90 semester hours or > equivalent) in an accredited college or university with the > following > distribution of courses-- > (A) For those whose training was completed before September 15, > 1963. At least 24 semester hours in chemistry and biology courses of > which-- > (1) At least 6 semester hours were in inorganic chemistry and at > least 3 semester hours were in other chemistry courses; and > (2) At least 12 semester hours in biology courses pertinent to the > medical sciences; or > (B) For those whose training was completed after September 14, 1963. > (1) 16 semester hours in chemistry courses that included at least 6 > semester hours in inorganic chemistry and that are acceptable toward > a > major in chemistry; > (2) 16 semester hours in biology courses that are pertinent to the > medical sciences and are acceptable toward a major in the biological > sciences; and > (3) 3 semester hours of mathematics; and > (ii) Has experience, training, or both, covering several fields of > medical laboratory work of at least 1 year and of such quality as to > provide him or her with education and training in medical technology > equivalent to that described in paragraphs (b)(1) and (2) of this > section; or > (5) With respect to individuals first qualifying before July 1, > 1971, the technologist-- > (i) Was performing the duties of a laboratory technologist at any > time between July 1, 1961, and January 1, 1968, and > (ii) Has had at least 10 years of pertinent laboratory experience > prior to January 1, 1968. (This required experience may be met by > substitution of education for experience); or > (6) Achieves a satisfactory grade in a proficiency examination > approved by HHS. > > Anthony Williams BSc. HT (ASCP) > Histotech Exchange LLC > 19 Whitmore Street > Lexington, VA 24450 > T 1 877 464 8911 > F 1 540 301 0071 > anthony@histotechexchange.com > www.histotechexchange.com > > > Hi Anthony, >> >> Grandfathering in to gross only means that you were grossing before that >> cutoff date. it matters not one bit if you have any certification as >> far >> as >> CLIA is concerned. That is how it was explained to me by an ex-CLIA >> inspector. >> >> Bonnie Whitaker >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >> anthony@histotechexchange.com >> Sent: Wednesday, January 09, 2008 1:21 PM >> To: Sharon.Davis-Devine >> Cc: histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] Who can perform which job functions >> >> >> Dear Sharon: >> >> This is as far as I am concerned very difficult to answer. This is >> because >> you have to bring in High Complexity Testing for the IHC. This is >> covered >> by >> CLIA 88 and that goes something like this. >> >> NOTE: The laboratory director may delegate the dissection of specimens >> to >> non-pathologist individuals; these individuals must be qualified as high >> complexity testing personnel under CLIA-88 regulations. The minimum >> training/experience required of such personnel is: >> >> 1. An earned associate degree in a laboratory science or medical >> laboratory >> technology, obtained from an accredited institution, OR 2. >> Education/training equivalent to the above that includes at least 60 >> semester hours or equivalent from an accredited institution. This >> education >> must include 24 semester hours of medical laboratory technology courses, >> OR >> 24 semester hours of science courses that includes 6 semester hours of >> chemistry, 6 semester hours of biology, and 12 semester hours of >> chemistry, >> biology or medical laboratory technology in any combination. >> In addition, the individual must have laboratory training including >> either >> completion of a clinical laboratory training program approved or >> accredited >> by the ABHES, the CAHEA, or other organization approved by HHS (note >> that >> this training may be included in the 60 semester hours listed above),OR >> at >> least 3 months documented laboratory training in each specialty in which >> the >> individual performs high complexity testing. >> >> In addition, the CLIA-88 regulations include exceptions for >> grandfathered individuals; these regulations (42CFR493.1489 and 1491) >> may >> be found at >> http://www.phppo.cdc.gov/clia/regs/subpart_m.aspx#493.1487 >> >> It is the responsibility of the laboratory director to determine whether >> an >> individual's education, training and experience satisfies the >> requirements >> of this checklist question. >> >> This checklist question applies only to laboratories subject to CLIA-88. >> >> And if you see at the end if you are not subject to CLIA-88 then you do >> not >> need to worry about it. Question is, are you subject to CLIA-88 under >> CAP >> regulations. What is the mandate for CLIA-88: >> >> Subpart A--General Provisions >> >> Source: 57 FR 7139, Feb. 28, 1992, unless otherwise noted. >> >> Sec. 493.1 Basis and scope. >> >> This part sets forth the conditions that all laboratories must meet to >> be >> certified to perform testing on human specimens under the Clinical >> Laboratory Improvement Amendments of 1988 (CLIA). It implements sections >> 1861 (e) and (j), the sentence following section 1861(s)(13), and >> 1902(a)(9) of the Social Security Act, and section 353 of the Public >> Health >> Service Act. This part applies to all laboratories as defined under >> ``laboratory'' in Sec. 493.2 of this part. This part also applies to >> laboratories seeking payment under the Medicare and Medicaid programs. >> The >> requirements are the same for Medicare approval as for CLIA >> certification. >> >> So as I understand it if you do not have an AA with 24 hours of a >> combination of Chemistry and Biology or were grandfathered in with a HTL >> (Not a HT), you should not be grossing or doing Immunos even if you are >> a >> certified HT. >> >> I apologize if I am wrong and please set me right. >> >> If someone out there can send me a flow diagram of how all the >> certifications is intertwined, what is mandated and what is organization >> based (i.e. ASCP) I would LOVE to get it. >> >> Yours truly, >> >> Anthony Williams BSc. HT (ASCP) >> Histotech Exchange LLC >> 19 Whitmore Street >> Lexington, VA 24450 >> T 1 877 464 8911 >> F 1 540 301 0071 >> anthony@histotechexchange.com >> www.histotechexchange.com >> >> >> Ok, all of you Histonetters I have another question for you. Who the >>> histology laboratory can perform these job functions: embedding, >>> cutting, performing special stains and IHC? Can a lab assistant >>> perform these duties if properly trained or do you have to be >>> classified as a Histotech in training? Can a Cytotech or Med Tech >>> perform such duties, again if properly trained? All opinions and >>> references to such requirements would be greatly appreciated. >>> >>> >>> >>> Sharon Davis-Devine, CT (ASCP) >>> >>> Cytology Supervisor >>> >>> Carle Clinic >>> >>> 602 West University >>> >>> Urbana, Illinois 61801 >>> >>> Phone: 217-383-3572 >>> >>> Email: sharon.davis-devine@carle.com >>> >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ________________________________________________________________________ > More new features than ever. Check out the new AOL Mail ! - > http://webmail.aol.com > From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Jan 10 03:42:08 2008 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Jan 10 03:42:32 2008 Subject: [Histonet] Response to On-Call pay Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F45B@wahtntex2.waht.swest.nhs.uk> So she only gets paid if she gets called in and that's only $49 per hour? $3.0 per hour if she doesn't get called in? What's that in real money (what's the exchange rate? $4 per ??) even at $2 per ? that's only ?25 per hour isn't it? Rates vary in the UK but no-one works more than 12 hours on call at any one time, must have a break between episodes of on call and gets about ?12,000 per annum ($24,000) for three sessions of no more than 12 hours on call per month. Works out about $100 per hour doesn't it? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Don?t be afraid to take a big step when one is indicated. You can?t cross a chasm in two small jumps. --Buckminster Fuller This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From JWEEMS <@t> sjha.org Thu Jan 10 06:11:13 2008 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Thu Jan 10 06:11:58 2008 Subject: [Histonet] Response to On-Call pay References: <86ADE4EB583CE64799A9924684A0FBBF0222F45B@wahtntex2.waht.swest.nhs.uk> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3204726B5E@sjhaexc02.sjha.org> Do you have any openings... I'll take call!j Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kemlo Rogerson Sent: Thu 1/10/2008 4:42 AM To: Cheri Miller; MICHELLE MCNEESE; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Response to On-Call pay So she only gets paid if she gets called in and that's only $49 per hour? $3.0 per hour if she doesn't get called in? What's that in real money (what's the exchange rate? $4 per ??) even at $2 per ? that's only ?25 per hour isn't it? Rates vary in the UK but no-one works more than 12 hours on call at any one time, must have a break between episodes of on call and gets about ?12,000 per annum ($24,000) for three sessions of no more than 12 hours on call per month. Works out about $100 per hour doesn't it? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Don't be afraid to take a big step when one is indicated. You can't cross a chasm in two small jumps. --Buckminster Fuller This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MICHELLE.MCNEESE <@t> childrens.com Thu Jan 10 06:30:34 2008 From: MICHELLE.MCNEESE <@t> childrens.com (MICHELLE MCNEESE) Date: Thu Jan 10 06:31:10 2008 Subject: [Histonet] Response to On-Call pay In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD3204726B5E@sjhaexc02.sjha.org> References: <86ADE4EB583CE64799A9924684A0FBBF0222F45B@wahtntex2.waht.swest.nhs.uk> <1CD6831EB9B26D45B0A3EAA79F7EBD3204726B5E@sjhaexc02.sjha.org> Message-ID: <4785BB8A020000290000F836@CNET3.CHILDRENS.COM> Everybody in the US knows exactly what my message was saying, and since the person doing the survey was US, we can just drop it now as it doesn't seem as though Kemlo is going to "get it", or he is purposely stubborn. Michelle L McNeese, HT (ASCP) Lead Tech, Histology Department of Pathology Childrens Medical Center (214) 456-6146 Email: michelle.mcneese@childrens.com >>> "Weems, Joyce" 01/10/08 6:11 AM >>> Do you have any openings... I'll take call!j Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kemlo Rogerson Sent: Thu 1/10/2008 4:42 AM To: Cheri Miller; MICHELLE MCNEESE; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Response to On-Call pay So she only gets paid if she gets called in and that's only $49 per hour? $3.0 per hour if she doesn't get called in? What's that in real money (what's the exchange rate? $4 per ??) even at $2 per ? that's only ?25 per hour isn't it? Rates vary in the UK but no-one works more than 12 hours on call at any one time, must have a break between episodes of on call and gets about ?12,000 per annum ($24,000) for three sessions of no more than 12 hours on call per month. Works out about $100 per hour doesn't it? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Don't be afraid to take a big step when one is indicated. You can't cross a chasm in two small jumps. --Buckminster Fuller This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Jan 10 06:34:02 2008 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Jan 10 06:34:26 2008 Subject: [Histonet] Response to On-Call pay Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F468@wahtntex2.waht.swest.nhs.uk> Got an opening for a Band 7 Biochemistry BMS; ?36,000 plus ?12,000 on Call. Interested? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Don?t be afraid to take a big step when one is indicated. You can?t cross a chasm in two small jumps. --Buckminster Fuller This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From rjbuesa <@t> yahoo.com Thu Jan 10 07:21:07 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 10 07:21:30 2008 Subject: [Histonet] Who can perform which job functions In-Reply-To: <44780C571F28624DBB446DE55C4D733A021E083A@EXCHANGEBE1.carle.com> Message-ID: <267393.11912.qm@web61211.mail.yahoo.com> Embedding determines HOW a piece of tissue if to be oriented, and that is a decision NOT for a lab assistant, no matter how well trained it is. Cutting is perhaps the CORE of the functions in histology. The one who is cutting gets to decide which sections to keep and which to discard FOR EVER and that is another decision that an assistant could not do properly. Performing special stains and IHC involves deciding also if the reaction took place or not. The above just to explain why I think that those are tasks that only a well trained histotech should perform. I realize that there are lab assistants with "exceptional" abilities, but to those what they have to do is to get a license. In short: NO I don't think that any of those tasks should be performed by an assistant. Ren? J. "Sharon.Davis-Devine" wrote: Ok, all of you Histonetters I have another question for you. Who the histology laboratory can perform these job functions: embedding, cutting, performing special stains and IHC? Can a lab assistant perform these duties if properly trained or do you have to be classified as a Histotech in training? Can a Cytotech or Med Tech perform such duties, again if properly trained? All opinions and references to such requirements would be greatly appreciated. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From godsgalnow <@t> aol.com Thu Jan 10 08:00:20 2008 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Thu Jan 10 08:01:10 2008 Subject: [Histonet] Who can perform which job functions In-Reply-To: <267393.11912.qm@web61211.mail.yahoo.com> Message-ID: <8CA21A845CC8B1F-BEC-29E0@mblk-d31.sysops.aol.com> Embedding is a critical step, but?is considered moderate complexity.? This would just separate functionality between a technician and a technologist. Roxanne -----Original Message----- From: Rene J Buesa To: Sharon.Davis-Devine ; histonet@lists.utsouthwestern.edu Sent: Thu, 10 Jan 2008 8:21 am Subject: Re: [Histonet] Who can perform which job functions Embedding determines HOW a piece of tissue if to be oriented, and that is a ecision NOT for a lab assistant, no matter how well trained it is. Cutting is perhaps the CORE of the functions in histology. The one who is utting gets to decide which sections to keep and which to discard FOR EVER and hat is another decision that an assistant could not do properly. Performing special stains and IHC involves deciding also if the reaction took lace or not. The above just to explain why I think that those are tasks that only a well rained histotech should perform. I realize that there are lab assistants with exceptional" abilities, but to those what they have to do is to get a license. In short: NO I don't think that any of those tasks should be performed by an ssistant. Ren? J. "Sharon.Davis-Devine" wrote: Ok, all of you Histonetters I have another question for you. Who the istology laboratory can perform these job functions: embedding, utting, performing special stains and IHC? Can a lab assistant perform hese duties if properly trained or do you have to be classified as a istotech in training? Can a Cytotech or Med Tech perform such duties, gain if properly trained? All opinions and references to such equirements would be greatly appreciated. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------- e a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. ______________________________________________ istonet mailing list istonet@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ More new features than ever. Check out the new AOL Mail ! - http://webmail.aol.com From vazquezr <@t> ohsu.edu Thu Jan 10 08:37:55 2008 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Thu Jan 10 08:38:40 2008 Subject: [Histonet] Response to On-Call pay Message-ID: Michelle, That was not very nice thing to say, Kemlo is only messing with you in good humor, he does get it. That is how I took it. Robyn From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Jan 10 08:43:43 2008 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Jan 10 08:44:09 2008 Subject: [Histonet] Response to On-Call pay Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F46F@wahtntex2.waht.swest.nhs.uk> It's the humour Robyn, rarely travels well, I sometimes wonder if the difference between Americans and the British is greater than that between men and women. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Don't be afraid to take a big step when one is indicated. You can't cross a chasm in two small jumps. --Buckminster Fuller This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From carl.hobbs <@t> kcl.ac.uk Thu Jan 10 08:44:39 2008 From: carl.hobbs <@t> kcl.ac.uk (carl hobbs) Date: Thu Jan 10 08:45:51 2008 Subject: [Histonet] re: Tuj 1 Message-ID: <004d01c85397$54370c40$112b5c9f@kclfrgvhctwh15> Hi naira. You give no information on your tissue preparation, immunomethod nor what you mean by "tuj 1" Do you mean the monoclonal antibody reagent, clone TUJ1, for neurone -specific beta III tubulin? You will find pictures and protocol here http://www.immunoportal.com/ Good luck Carl Hobbs Histology Manager Wolfson Centre for Age-Related Diseases Wolfson Wing Hodgkin Building Guys Campus Kings College London London SE1 1UL 020 78486810 London From Barry.R.Rittman <@t> uth.tmc.edu Thu Jan 10 08:59:24 2008 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Thu Jan 10 08:59:47 2008 Subject: [Histonet] Who can perform which job functions In-Reply-To: <8CA21A845CC8B1F-BEC-29E0@mblk-d31.sysops.aol.com> Message-ID: I don't generally get involved in this type of discussion; however I feel that an important point has been missed. No matter how well an individual is trained it is their attitude rather than their experience that is usually the most important factor in how they carry out tasks. It is true that it takes a considerable amount of skill to cut and embed tissues, however it is how each block and section is mentally approached that usually determines the final result. There is a danger in carry out repetitive tasks day after day to become complacent and to not do your best work. Some of the best advice that I was given was to approach every job no matter how large or small or menial with the attitude of always doing your best. Sometimes (actually often) I am accused of being a anal but I find that this mental attitude is very rewarding to me personally. I do believe that you should be issued with a cattle prod or Taser to use liberally if the pathologist insists on looking over your shoulder while you are working! Barry From JWEEMS <@t> sjha.org Thu Jan 10 09:03:16 2008 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Thu Jan 10 09:03:41 2008 Subject: [Histonet] Response to On-Call pay In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF0222F46F@wahtntex2.waht.swest.nhs.uk> References: <86ADE4EB583CE64799A9924684A0FBBF0222F46F@wahtntex2.waht.swest.nhs.uk> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F485C@sjhaexc02.sjha.org> Our roots have become so mixed up, we forget how dry your humour is - the funniest kind! j -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk] Sent: Thursday, January 10, 2008 9:44 AM To: Robyn Vazquez; MICHELLE.MCNEESE@childrens.com; histonet@lists.utsouthwestern.edu; cmiller@physlab.com; Weems, Joyce Subject: RE: [Histonet] Response to On-Call pay It's the humour Robyn, rarely travels well, I sometimes wonder if the difference between Americans and the British is greater than that between men and women. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Don't be afraid to take a big step when one is indicated. You can't cross a chasm in two small jumps. --Buckminster Fuller This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From MICHELLE.MCNEESE <@t> childrens.com Thu Jan 10 09:10:38 2008 From: MICHELLE.MCNEESE <@t> childrens.com (MICHELLE MCNEESE) Date: Thu Jan 10 09:11:21 2008 Subject: [Histonet] Response to On-Call pay In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF0222F46F@wahtntex2.waht.swest.nhs.uk> References: <86ADE4EB583CE64799A9924684A0FBBF0222F46F@wahtntex2.waht.swest.nhs.uk> Message-ID: <4785E10E020000290000F85F@CNET3.CHILDRENS.COM> My most sincere apologies, Kemlo, for being the one to not "get it". And, I will also admit, being quite stubborn Michelle L McNeese, HT (ASCP) Lead Tech, Histology Department of Pathology Childrens Medical Center (214) 456-6146 Email: michelle.mcneese@childrens.com >>> "Kemlo Rogerson" 01/10/08 8:43 AM >>> It's the humour Robyn, rarely travels well, I sometimes wonder if the difference between Americans and the British is greater than that between men and women. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Don't be afraid to take a big step when one is indicated. You can't cross a chasm in two small jumps. --Buckminster Fuller This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From GDawson <@t> dynacaremilwaukee.com Thu Jan 10 09:42:55 2008 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Thu Jan 10 09:43:24 2008 Subject: [Histonet] Response to On-Call pay In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF0222F46F@wahtntex2.waht.swest.nhs.uk> Message-ID: Humor IS shared by both Americans and British, as is misinterpretation, misunderstanding and becoming indignant/offended over the strangest things. It isn't an Americans vs. the British thing, it's a histonet thing. Over-reactions are rampant and one should be aware that having a sense of humor should have a "Use at your own risk" sign when utilizing it on this forum. In every group there will always be a number of folks more interested in attacking what is being said by others rather than contributing something of their own. Each year this trend gets a bit worse & I find myself "replying to all" less and less. Posting to an individual usually causes less of a stir so that is the road I take much more these days. Just my .01 Euros, Glen Dawson IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Kemlo Rogerson Sent: Thursday, January 10, 2008 8:44 AM To: Robyn Vazquez; MICHELLE.MCNEESE@childrens.com; histonet@lists.utsouthwestern.edu; cmiller@physlab.com; JWEEMS@sjha.org Subject: RE: [Histonet] Response to On-Call pay It's the humour Robyn, rarely travels well, I sometimes wonder if the difference between Americans and the British is greater than that between men and women. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Don't be afraid to take a big step when one is indicated. You can't cross a chasm in two small jumps. --Buckminster Fuller This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Thu Jan 10 09:46:16 2008 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Thu Jan 10 09:46:44 2008 Subject: [Histonet] Response to On-Call pay Message-ID: <5C0BED61F529364E86309CADEA63FEF2F3AF33@TRFT-EX01.xRothGen.nhs.uk> Well said. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: 10 January 2008 15:43 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Response to On-Call pay Humor IS shared by both Americans and British, as is misinterpretation, misunderstanding and becoming indignant/offended over the strangest things. It isn't an Americans vs. the British thing, it's a histonet thing. Over-reactions are rampant and one should be aware that having a sense of humor should have a "Use at your own risk" sign when utilizing it on this forum. In every group there will always be a number of folks more interested in attacking what is being said by others rather than contributing something of their own. Each year this trend gets a bit worse & I find myself "replying to all" less and less. Posting to an individual usually causes less of a stir so that is the road I take much more these days. Just my .01 Euros, Glen Dawson IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Kemlo Rogerson Sent: Thursday, January 10, 2008 8:44 AM To: Robyn Vazquez; MICHELLE.MCNEESE@childrens.com; histonet@lists.utsouthwestern.edu; cmiller@physlab.com; JWEEMS@sjha.org Subject: RE: [Histonet] Response to On-Call pay It's the humour Robyn, rarely travels well, I sometimes wonder if the difference between Americans and the British is greater than that between men and women. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Don't be afraid to take a big step when one is indicated. You can't cross a chasm in two small jumps. --Buckminster Fuller This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ###################################################################### This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. ###################################################################### From ccpath <@t> gmail.com Thu Jan 10 09:50:49 2008 From: ccpath <@t> gmail.com (j k) Date: Thu Jan 10 09:51:13 2008 Subject: [Histonet] Response to On-Call pay In-Reply-To: <5C0BED61F529364E86309CADEA63FEF2F3AF33@TRFT-EX01.xRothGen.nhs.uk> References: <5C0BED61F529364E86309CADEA63FEF2F3AF33@TRFT-EX01.xRothGen.nhs.uk> Message-ID: They have better beer, and consequently, better humor. jim On 1/10/08, Marshall Terry Dr, Consultant Histopathologist < Terry.Marshall@rothgen.nhs.uk> wrote: > > Well said. > > Terry > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, > Glen > Sent: 10 January 2008 15:43 > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Response to On-Call pay > > Humor IS shared by both Americans and British, as is misinterpretation, > misunderstanding and becoming indignant/offended over the strangest > things. > > It isn't an Americans vs. the British thing, it's a histonet thing. > Over-reactions are rampant and one should be aware that having a sense > of humor should have a "Use at your own risk" sign when utilizing it on > this forum. In every group there will always be a number of folks more > interested in attacking what is being said by others rather than > contributing something of their own. > > Each year this trend gets a bit worse & I find myself "replying to all" > less and less. Posting to an individual usually causes less of a stir > so that is the road I take much more these days. > > Just my .01 Euros, > > Glen Dawson > IHC Manager > Milwaukee, WI > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Kemlo > Rogerson > Sent: Thursday, January 10, 2008 8:44 AM > To: Robyn Vazquez; MICHELLE.MCNEESE@childrens.com; > histonet@lists.utsouthwestern.edu; cmiller@physlab.com; JWEEMS@sjha.org > Subject: RE: [Histonet] Response to On-Call pay > > > It's the humour Robyn, rarely travels well, I sometimes wonder if the > difference between Americans and the British is greater than that > between men and women. > > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > Don't be afraid to take a big step when one is indicated. You can't > cross a chasm in two small jumps. --Buckminster Fuller From ree3 <@t> leicester.ac.uk Thu Jan 10 09:53:29 2008 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Thu Jan 10 09:53:53 2008 Subject: FW: [Histonet] Response to On-Call pay Message-ID: -----Original Message----- From: Edwards, R.E. Sent: 10 January 2008 15:18 To: 'MICHELLE MCNEESE' Subject: RE: [Histonet] Response to On-Call pay Not to worry in my experience humor or is it humour rarely travels well, particularly within eeeemails. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MICHELLE MCNEESE Sent: 10 January 2008 15:11 To: histonet@lists.utsouthwestern.edu; Robyn Vazquez; cmiller@physlab.com; JWEEMS@sjha.org; Kemlo Rogerson Subject: RE: [Histonet] Response to On-Call pay My most sincere apologies, Kemlo, for being the one to not "get it". And, I will also admit, being quite stubborn Michelle L McNeese, HT (ASCP) Lead Tech, Histology Department of Pathology Childrens Medical Center (214) 456-6146 Email: michelle.mcneese@childrens.com >>> "Kemlo Rogerson" 01/10/08 8:43 AM >>> >>> It's the humour Robyn, rarely travels well, I sometimes wonder if the difference between Americans and the British is greater than that between men and women. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Don't be afraid to take a big step when one is indicated. You can't cross a chasm in two small jumps. --Buckminster Fuller This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Thu Jan 10 09:53:40 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Thu Jan 10 09:53:57 2008 Subject: [Histonet] renal tubules References: <000a01c85317$ed4c6860$6501a8c0@Patsyoffice> Message-ID: <004101c853a0$f86fd310$6501a8c0@DHXTS541> Maybe the same panel of special stains commonly used for human renal biopsies. We did H&E, Massons Trichrome, PASH and possibly Jones Basement Membrane staining. You would have to cut thin paraffin sections, 2 um at the most for the Jones Methenamine silver. Gayle M. Callis HT/HTL/MT(ASCP) Bozeman MT ----- Original Message ----- From: "Patsy Ruegg" To: "'Histonet'" Sent: Wednesday, January 09, 2008 4:32 PM Subject: [Histonet] renal tubules > Folks, > > I have an investigator that wants to demonstrate damage to renal tubules > in > rats caused by giving them uranyl acetate. Can you recommend special > stains > for that? Would it be something like PAS? Also are there antibodies we > could use on ffpe rat kidneys that might help with this project? > > Best regards, > > Patsy > > > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech, LLC > Fitzsimmons BioScience Park > 12635 Montview Blvd. Suite 215 > Aurora, CO 80010 > P-720-859-4060 > F-720-859-4110 > wk email pruegg@ihctech.net > web site www.ihctech.net > > > > > This email is confidential and intended solely for the use of the > Person(s) > ('the intended recipient') to whom it was addressed. Any views or opinions > presented are solely those of the author. It may contain information that > is > privileged & confidential within the meaning of applicable law. > Accordingly > any dissemination, distribution, copying, or other use of this message, or > any of its contents, by any person other than the intended recipient may > constitute a breach of civil or criminal law and is strictly prohibited. > If > you are NOT the intended recipient please contact the sender and dispose > of > this e-mail as soon as possible. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud <@t> holyredeemer.com Thu Jan 10 09:54:41 2008 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Thu Jan 10 09:55:07 2008 Subject: [Histonet] Who can perform Histology Duties In-Reply-To: Message-ID: "Sharon.Davis-Devine" wrote: Ok, all of you Histonetters I have another question for you. Who the histology laboratory can perform these job functions: embedding, cutting, performing special stains and IHC? Can a lab assistant perform these duties if properly trained or do you have to be classified as a Histotech in training? Can a Cytotech or Med Tech perform such duties, again if properly trained? All opinions and references to such requirements would be greatly appreciated. Though some may not like to admit it, ANYONE properly trained and documented as competent can perform the duties listed above. The only exception is for those states that have licensure requirements for the performance of Histological duties, and then, the licensure requirements must be met. IHC is still a stain. The interpretation of that stain by the pathologist is the high complexity part of the test. Anyone properly trained and documented as competent can stain IHC. Even grossing has now been re-defined by CAP to allow submission of smaller tissues by personnel without the qualifications for High Complexity Testing (see below) (CAP checklist question and notes: ANP.11600) 1) Processing is defined as a tissue examination limited to description, inking and cutting of the specimen (if applicable), and submission of the entire specimen to histology. Tissue processing can be performed according to standardized protocols. Processing is generally limited to small specimens (skin ellipses, small biopsies, curettings, etc.) and does not require knowledge of anatomy. 2) Grossing (or gross examination) is defined as a tissue examination requiring a greater exercise of judgment and a knowledge of anatomy. Dissection of the specimen and selection of tissue samples for submission to histology are generally required. The specimen description is not necessarily standardized. Please save your flames. The scenerio of a Non-Histotech embedding, cutting, and staining my surgical tissue does not thrill me, but that was not her question. Maybe the reality of the answer is why many Histology Labs have always been considered the evil red-headed stepchildren of the Lab. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supv. Laboratory, Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3689 --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Jan 10 10:07:41 2008 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Jan 10 10:08:07 2008 Subject: [Histonet] Who can perform Histology Duties Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F476@wahtntex2.waht.swest.nhs.uk> Please save your flames. The scenerio of a Non-Histotech embedding, cutting, and staining my surgical tissue does not thrill me, but that was not her question. Maybe the reality of the answer is why many Histology Labs have always been considered the evil red-headed stepchildren of the Lab. Terri I think you are correct in that as long as someone is properly trained, the training is revisited regularly and there is adequate supervision and the professional groups are in agreement Non-BMS (Non-Histotech) Staff can do many jobs in Histology and maybe Non Medical Staff (BMS) can carry out some interpretation. I would have no issues with a Non-Histotech (Non-BMS) doing as you say with proper supervision by a HPC registered supervisor. But where does it end? Do you need a medical degree to identify vas, appendix or gall bladder? You need the training to know when you have reached the limits of your knowledge and when to refer to someone better able to report a disease and I concede that is probably a Medic. Proper training can allow Histotech's to report normal, maybe non-normal requires medical insight, what do you think? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Don't be afraid to take a big step when one is indicated. You can't cross a chasm in two small jumps. --Buckminster Fuller This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From SDrew <@t> uwhealth.org Thu Jan 10 10:29:56 2008 From: SDrew <@t> uwhealth.org (Drew Sally A.) Date: Thu Jan 10 10:30:18 2008 Subject: [Histonet] Counterstain on Ventana stainers Message-ID: <3F328377AF4E23438E78234752652CE105D525F8@uwhis-xchng7.uwhis.hosp.wisc.edu> Is anyone using a non-Ventana hematoxylin counterstain on their immunostainers? If so, what works best for you in terms of intensity and stability. Just looking at a bit of cost-cutting... Thank you for your time! Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 From Terry.Marshall <@t> rothgen.nhs.uk Thu Jan 10 10:30:40 2008 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Thu Jan 10 10:31:05 2008 Subject: [Histonet] Who can perform Histology Duties Message-ID: <5C0BED61F529364E86309CADEA63FEF2F3AF35@TRFT-EX01.xRothGen.nhs.uk> "Proper training can allow Histotech's to report normal, ....." There was a bizarre discussion on the histopathology discussion group recently, around reporting tissue as "normal". An amazing number of pathologists never used the word, but preferred some sort of mealy mouthed evasion. Within normal limits, no definite lesion seen ... That sort of thing. Indeed one very competent pathologist claimed he could not recognise normal! Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: 10 January 2008 16:08 To: Terri Braud; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Who can perform Histology Duties Please save your flames. The scenerio of a Non-Histotech embedding, cutting, and staining my surgical tissue does not thrill me, but that was not her question. Maybe the reality of the answer is why many Histology Labs have always been considered the evil red-headed stepchildren of the Lab. Terri I think you are correct in that as long as someone is properly trained, the training is revisited regularly and there is adequate supervision and the professional groups are in agreement Non-BMS (Non-Histotech) Staff can do many jobs in Histology and maybe Non Medical Staff (BMS) can carry out some interpretation. I would have no issues with a Non-Histotech (Non-BMS) doing as you say with proper supervision by a HPC registered supervisor. But where does it end? Do you need a medical degree to identify vas, appendix or gall bladder? You need the training to know when you have reached the limits of your knowledge and when to refer to someone better able to report a disease and I concede that is probably a Medic. Proper training can allow Histotech's to report normal, maybe non-normal requires medical insight, what do you think? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Don't be afraid to take a big step when one is indicated. You can't cross a chasm in two small jumps. --Buckminster Fuller This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ###################################################################### This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. ###################################################################### From bwhitaker <@t> brownpathology.com Thu Jan 10 10:37:23 2008 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Thu Jan 10 10:33:21 2008 Subject: [Histonet] Who can perform Histology Duties In-Reply-To: Message-ID: <006101c853a7$14352450$3601a8c0@brownpathology.net> Hi Terri, CAP has changed it's definition of grossing but most CAP labs are also required to follow CLIA, even though they aren't inspected by CLIA. Don't forget about that if you bill Medicare. They COULD come in and do an unannounced inspection and zap you if your grossers don't meet the CLIA standards for high complexity testing one way or another. Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Thursday, January 10, 2008 9:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Who can perform Histology Duties "Sharon.Davis-Devine" wrote: Ok, all of you Histonetters I have another question for you. Who the histology laboratory can perform these job functions: embedding, cutting, performing special stains and IHC? Can a lab assistant perform these duties if properly trained or do you have to be classified as a Histotech in training? Can a Cytotech or Med Tech perform such duties, again if properly trained? All opinions and references to such requirements would be greatly appreciated. Though some may not like to admit it, ANYONE properly trained and documented as competent can perform the duties listed above. The only exception is for those states that have licensure requirements for the performance of Histological duties, and then, the licensure requirements must be met. IHC is still a stain. The interpretation of that stain by the pathologist is the high complexity part of the test. Anyone properly trained and documented as competent can stain IHC. Even grossing has now been re-defined by CAP to allow submission of smaller tissues by personnel without the qualifications for High Complexity Testing (see below) (CAP checklist question and notes: ANP.11600) 1) Processing is defined as a tissue examination limited to description, inking and cutting of the specimen (if applicable), and submission of the entire specimen to histology. Tissue processing can be performed according to standardized protocols. Processing is generally limited to small specimens (skin ellipses, small biopsies, curettings, etc.) and does not require knowledge of anatomy. 2) Grossing (or gross examination) is defined as a tissue examination requiring a greater exercise of judgment and a knowledge of anatomy. Dissection of the specimen and selection of tissue samples for submission to histology are generally required. The specimen description is not necessarily standardized. Please save your flames. The scenerio of a Non-Histotech embedding, cutting, and staining my surgical tissue does not thrill me, but that was not her question. Maybe the reality of the answer is why many Histology Labs have always been considered the evil red-headed stepchildren of the Lab. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supv. Laboratory, Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3689 ---------------------------------------------------------------------------- ----- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Tbarnhart <@t> primecare.org Thu Jan 10 10:50:44 2008 From: Tbarnhart <@t> primecare.org (Barnhart, Tammy) Date: Thu Jan 10 10:49:49 2008 Subject: [Histonet] Mart-1/Melan-A as a stain for melanoma sentinel nodes Message-ID: <4F0B7161A6CD524FAD8017D52E1553407AE610@exchangent> We are having some debate about which marker to use for our melanoma sentinel node screens. We have been using Mart-1, but recently had a case where we think mast cells were stained. We can find no literature that indicates that Mart-1 will stain mast cells. We use Lab Vision's Mart-1/Melan-a AB-3 (clone M27C10+M2-9e3). We ran a Mast Cell tryptase to see what stained, and all the cells that stained with the Mart-1 stained plus a bunch of others. So if the Mart-1 is staining mast cells, it is only staining a subset of them. We really think that these are mast cells and not melanoma, but would like to see some literature to support this. So two questions: 1) What are people using as a screening antibody and 2) Does anyone have a reference that indicates that this antibody will stain mast cells. Thanks in advance for your help. Tammy Barnhart, BS, HTL(ASCP) Anatomic Pathology Supervisor St. Alexius Medical Center Bismarck, ND From kgrobert <@t> rci.rutgers.edu Thu Jan 10 10:59:15 2008 From: kgrobert <@t> rci.rutgers.edu (Kathleen Roberts) Date: Thu Jan 10 10:51:36 2008 Subject: [Histonet] Response to humor differences, etc. In-Reply-To: References: Message-ID: <47864EE3.5010009@rci.rutgers.edu> Actually, in my experience, it isn't just a Histonet thing, it's an ONLINE thing. No matter what forum or list you're on, there is always misunderstanding, fights and flaming b/c two crucial things are missing in online forums: tone of voice and facial expressions. (Yes, even when those are present, misunderstandings, etc. still occur.) Emoticons don't even begin to cut it, and sometimes when a person is reading something, his/her mood, opinions, experience, etc. will "color" what's being read and thus influence the person's understanding of what was written, which the author never intended and can't control. I can't tell you how many times that has happened to me. Kathleen Roberts Dawson, Glen wrote: >Humor IS shared by both Americans and British, as is misinterpretation, misunderstanding and becoming indignant/offended over the strangest things. > >It isn't an Americans vs. the British thing, it's a histonet thing. Over-reactions are rampant and one should be aware that having a sense of humor should have a "Use at your own risk" sign when utilizing it on this forum. In every group there will always be a number of folks more interested in attacking what is being said by others rather than contributing something of their own. > >Each year this trend gets a bit worse & I find myself "replying to all" less and less. Posting to an individual usually causes less of a stir so that is the road I take much more these days. > >Just my .01 Euros, > >Glen Dawson >IHC Manager >Milwaukee, WI > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Kemlo >Rogerson >Sent: Thursday, January 10, 2008 8:44 AM >To: Robyn Vazquez; MICHELLE.MCNEESE@childrens.com; >histonet@lists.utsouthwestern.edu; cmiller@physlab.com; JWEEMS@sjha.org >Subject: RE: [Histonet] Response to On-Call pay > > >It's the humour Robyn, rarely travels well, I sometimes wonder if the >difference between Americans and the British is greater than that >between men and women. > > >Kemlo Rogerson >Pathology Manager >DD 01934 647057 or extension 3311 >Mob 07749 754194; Pager 07659 597107; >Don't be afraid to take a big step when one is indicated. You can't >cross a chasm in two small jumps. --Buckminster Fuller > >This e-mail is confidential and privileged. If you are not the intended >recipient please accept my apologies; please do not disclose, copy or >distribute information in this e-mail or take any action in reliance on >its contents: to do so is strictly prohibited and may be unlawful. >Please inform me that this message has gone astray before deleting it. >Thank you for your co-operation > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From tp2 <@t> medicine.wisc.edu Thu Jan 10 10:53:24 2008 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Thu Jan 10 10:54:14 2008 Subject: [Histonet] Counterstain on Ventana stainers Message-ID: <4785F925020000DF00004A23@gwmail.medicine.wisc.edu> I use Biocare's CAT hematoxylin mixed 50/50 with distilled water for one minute followed by a TBS-T rinse on my Lab Vision Autostainer 360. It seems to do a pretty good job. Tom Pier >>> "Drew Sally A." 01/10/08 10:29 AM >>> Is anyone using a non-Ventana hematoxylin counterstain on their immunostainers? If so, what works best for you in terms of intensity and stability. Just looking at a bit of cost-cutting... Thank you for your time! Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JCollins <@t> palmbeachpath.com Thu Jan 10 10:55:03 2008 From: JCollins <@t> palmbeachpath.com (Judy Collins) Date: Thu Jan 10 10:55:27 2008 Subject: [Histonet] Who Can Perform Histologoy Duties Message-ID: Bonnie is correct regarding the CLIA rules but one thing puzzles me: Supposedly in order to inspect instead of CLIA as CAP is authorized to do, their regulations must be at least as stringent as CLIA if not more so. This being so, how can CAP change its grossing regulation to one less stringent than CLIA? Judy Collins, CT,HT(ASCP) Palm Beach Pathology Hi Terri, CAP has changed it's definition of grossing but most CAP labs are also required to follow CLIA, even though they aren't inspected by CLIA. Don't forget about that if you bill Medicare. They COULD come in and do an unannounced inspection and zap you if your grossers don't meet the CLIA standards for high complexity testing one way or another. Bonnie Whitaker From jmahoney <@t> alegent.org Thu Jan 10 08:56:54 2008 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Thu Jan 10 10:59:55 2008 Subject: [Histonet] Who can perform which job functions In-Reply-To: <2345.65.40.219.156.1199927481.squirrel@host7.wfdns.com> References: <000701c85303$30846750$3601a8c0@brownpathology.net> <2345.65.40.219.156.1199927481.squirrel@host7.wfdns.com> Message-ID: <4785DDD60200003C000271F9@gwia.alegent.org> I think there is some confusion about the difference between the performance/staining of the test and the interpretation/quantifying/resulting of the test. The performance or the staining is not the part of IHC, FISH, etc that makes it high complexity. The interpretation/resulting is the high complexity part. No different than the performance of creating H&E slides which is not high complexity. Jan Omaha, NE From tbraud <@t> holyredeemer.com Thu Jan 10 11:02:31 2008 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Thu Jan 10 11:03:01 2008 Subject: [Histonet] Who can perform Histology Duties In-Reply-To: <006101c853a7$14352450$3601a8c0@brownpathology.net> Message-ID: CLIA considers "grossing" to be a high complexity test. The transference of the all tissue received in a container to a cassette, using a standard dictation is not defined as grossing, but as "processing", which is not a high complexity test. CLIA uses this clarification but it must be clearly defined in your procedure. -----Original Message----- From: Bonnie Whitaker [mailto:bwhitaker@brownpathology.com] Sent: Thursday, January 10, 2008 11:37 AM To: Terri Braud; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Who can perform Histology Duties Hi Terri, CAP has changed it's definition of grossing but most CAP labs are also required to follow CLIA, even though they aren't inspected by CLIA. Don't forget about that if you bill Medicare. They COULD come in and do an unannounced inspection and zap you if your grossers don't meet the CLIA standards for high complexity testing one way or another. Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Thursday, January 10, 2008 9:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Who can perform Histology Duties "Sharon.Davis-Devine" wrote: Ok, all of you Histonetters I have another question for you. Who the histology laboratory can perform these job functions: embedding, cutting, performing special stains and IHC? Can a lab assistant perform these duties if properly trained or do you have to be classified as a Histotech in training? Can a Cytotech or Med Tech perform such duties, again if properly trained? All opinions and references to such requirements would be greatly appreciated. Though some may not like to admit it, ANYONE properly trained and documented as competent can perform the duties listed above. The only exception is for those states that have licensure requirements for the performance of Histological duties, and then, the licensure requirements must be met. IHC is still a stain. The interpretation of that stain by the pathologist is the high complexity part of the test. Anyone properly trained and documented as competent can stain IHC. Even grossing has now been re-defined by CAP to allow submission of smaller tissues by personnel without the qualifications for High Complexity Testing (see below) (CAP checklist question and notes: ANP.11600) 1) Processing is defined as a tissue examination limited to description, inking and cutting of the specimen (if applicable), and submission of the entire specimen to histology. Tissue processing can be performed according to standardized protocols. Processing is generally limited to small specimens (skin ellipses, small biopsies, curettings, etc.) and does not require knowledge of anatomy. 2) Grossing (or gross examination) is defined as a tissue examination requiring a greater exercise of judgment and a knowledge of anatomy. Dissection of the specimen and selection of tissue samples for submission to histology are generally required. The specimen description is not necessarily standardized. Please save your flames. The scenerio of a Non-Histotech embedding, cutting, and staining my surgical tissue does not thrill me, but that was not her question. Maybe the reality of the answer is why many Histology Labs have always been considered the evil red-headed stepchildren of the Lab. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supv. Laboratory, Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3689 ---------------------------------------------------------------------------- ----- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From ree3 <@t> leicester.ac.uk Thu Jan 10 11:09:07 2008 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Thu Jan 10 11:09:35 2008 Subject: [Histonet] Response to humor differences, etc. In-Reply-To: <47864EE3.5010009@rci.rutgers.edu> References: <47864EE3.5010009@rci.rutgers.edu> Message-ID: "Emoticons", weren't they the baddies in an early episode of Dr. Who? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathleen Roberts Sent: 10 January 2008 16:59 To: 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Response to humor differences, etc. Actually, in my experience, it isn't just a Histonet thing, it's an ONLINE thing. No matter what forum or list you're on, there is always misunderstanding, fights and flaming b/c two crucial things are missing in online forums: tone of voice and facial expressions. (Yes, even when those are present, misunderstandings, etc. still occur.) Emoticons don't even begin to cut it, and sometimes when a person is reading something, his/her mood, opinions, experience, etc. will "color" what's being read and thus influence the person's understanding of what was written, which the author never intended and can't control. I can't tell you how many times that has happened to me. Kathleen Roberts Dawson, Glen wrote: >Humor IS shared by both Americans and British, as is misinterpretation, misunderstanding and becoming indignant/offended over the strangest things. > >It isn't an Americans vs. the British thing, it's a histonet thing. Over-reactions are rampant and one should be aware that having a sense of humor should have a "Use at your own risk" sign when utilizing it on this forum. In every group there will always be a number of folks more interested in attacking what is being said by others rather than contributing something of their own. > >Each year this trend gets a bit worse & I find myself "replying to all" less and less. Posting to an individual usually causes less of a stir so that is the road I take much more these days. > >Just my .01 Euros, > >Glen Dawson >IHC Manager >Milwaukee, WI > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Kemlo >Rogerson >Sent: Thursday, January 10, 2008 8:44 AM >To: Robyn Vazquez; MICHELLE.MCNEESE@childrens.com; >histonet@lists.utsouthwestern.edu; cmiller@physlab.com; JWEEMS@sjha.org >Subject: RE: [Histonet] Response to On-Call pay > > >It's the humour Robyn, rarely travels well, I sometimes wonder if the >difference between Americans and the British is greater than that >between men and women. > > >Kemlo Rogerson >Pathology Manager >DD 01934 647057 or extension 3311 >Mob 07749 754194; Pager 07659 597107; >Don't be afraid to take a big step when one is indicated. You can't >cross a chasm in two small jumps. --Buckminster Fuller > >This e-mail is confidential and privileged. If you are not the intended >recipient please accept my apologies; please do not disclose, copy or >distribute information in this e-mail or take any action in reliance on >its contents: to do so is strictly prohibited and may be unlawful. >Please inform me that this message has gone astray before deleting it. >Thank you for your co-operation > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Thu Jan 10 11:19:29 2008 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Thu Jan 10 11:19:53 2008 Subject: [Histonet] Agree to disagree Message-ID: <0E394B648E5284478A6CCB78E5AFDA27056353B9@hpes1.HealthPartners.int> I agree with Glen inasmuch as I do not do a lot of "post" responses due to being attacked! Constructive criticism is great and offering gentle advice is even better, but not "putting one down" because they do not know as much as someone else may or have a different viewpoint!! I still LOVE the histonet and appreciate all of the great wisdom instilled in me by those of you who keep responding to my inquiries and others!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From GDawson <@t> dynacaremilwaukee.com Thu Jan 10 11:23:52 2008 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Thu Jan 10 11:24:18 2008 Subject: [Histonet] Mart-1/Melan-A as a stain for melanoma sentinel nodes In-Reply-To: <4F0B7161A6CD524FAD8017D52E1553407AE610@exchangent> Message-ID: Tammy, I use a cocktail of Mart-1, Melan-A & Tyrosinase that we named the MCW (Medical College of Wisconsin) Melanoma Cocktail. If you google it you should find our paper online and I do believe that my Pathologist addresses some Mast cell staining issues in it. The triple antibody is excellent as a screener as it covers more epitopes than any one of the three does individually. Suggested staining methods are in the paper as well. Best of Luck, Glen Dawson IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Barnhart, Tammy Sent: Thursday, January 10, 2008 10:51 AM To: Histonet (E-mail) Subject: [Histonet] Mart-1/Melan-A as a stain for melanoma sentinel nodes We are having some debate about which marker to use for our melanoma sentinel node screens. We have been using Mart-1, but recently had a case where we think mast cells were stained. We can find no literature that indicates that Mart-1 will stain mast cells. We use Lab Vision's Mart-1/Melan-a AB-3 (clone M27C10+M2-9e3). We ran a Mast Cell tryptase to see what stained, and all the cells that stained with the Mart-1 stained plus a bunch of others. So if the Mart-1 is staining mast cells, it is only staining a subset of them. We really think that these are mast cells and not melanoma, but would like to see some literature to support this. So two questions: 1) What are people using as a screening antibody and 2) Does anyone have a reference that indicates that this antibody will stain mast cells. Thanks in advance for your help. Tammy Barnhart, BS, HTL(ASCP) Anatomic Pathology Supervisor St. Alexius Medical Center Bismarck, ND _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Jan 10 11:41:38 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 10 11:42:01 2008 Subject: [Histonet] Who can perform Histology Duties In-Reply-To: Message-ID: <463239.48520.qm@web61219.mail.yahoo.com> In the same way a "well trained woo-doo priest" can perform surgery? What about the knowledge background? Irrelevant? Ren? J. Terri Braud wrote: --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From POWELL_SA <@t> Mercer.edu Thu Jan 10 11:52:13 2008 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Thu Jan 10 11:56:23 2008 Subject: [Histonet] Mohs Tech position in Georgia Message-ID: <01MPXG4PEYQE003MTI@Macon2.Mercer.edu> Please Do Not reply to me. I am posting this for a friend. Please contact the Dr. Lane or Renee Blanton at the numbers below. Needed immediately Certified Mohs histotech Exciting new Mohs surgery practice in Columbus, Georgia Part-time or full-time possible Competitive pay Excellent benefits Contact: Joshua Lane, M.D. at 706.573.6545 or Renee Blanton at 706.322.1717 From dmnelson <@t> iastate.edu Thu Jan 10 12:02:56 2008 From: dmnelson <@t> iastate.edu (Diane Gerjets) Date: Thu Jan 10 12:03:19 2008 Subject: [Histonet] alkaline phosphatase stain Message-ID: <200801101802.m0AI2rNX011671@despam-11.iastate.edu> Hello, We have a pathologist that would like to find an alkaline phosphatase stain that indicates osteosarcoma on a cytology slide. She is wanting it to be a special stain and not an immunohistochemistry stain. We would appreciate any help that you could give us. Thank-you, Diane From gvdobbin <@t> ihis.org Thu Jan 10 12:17:52 2008 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Thu Jan 10 12:18:38 2008 Subject: [Histonet] Counterstain on Ventana stainers Message-ID: Hi Sally, The only one to choose in my mind is one that never needs filtering (so that once the reagent dispenser is filled you can forget about it until it is empty). And the only one on the market currently that does not need filtering is the Surgipath SelecTech hematoxylin. Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 There is some merit in doing the right thing rather badly, but absolutely none in doing the wrong thing excellently! >>> "Drew Sally A." 1/10/2008 12:29:56 PM >>> Is anyone using a non-Ventana hematoxylin counterstain on their immunostainers? If so, what works best for you in terms of intensity and stability. Just looking at a bit of cost-cutting... Thank you for your time! Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ayant pour objet une personne ou un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer compl?tement de votre syst?me informatique. From laurie.colbert <@t> huntingtonhospital.com Thu Jan 10 12:22:03 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Thu Jan 10 12:22:25 2008 Subject: [Histonet] CJD and CSF Fluids Message-ID: <57BE698966D5C54EAE8612E8941D76830235109A@EXCHANGE3.huntingtonhospital.com> The Clinical Lab is asking about disposing of CSF's from CJD or suspected CJD cases. They are saying to incinerate the left over specimen. I have a procedure for handling tissue, but not fluids. My procedure says to incinerate paraffin scraps and unused sections (this is after the tissue has been through formalin for 2-7 days and in formic acid for 1 hour). Additionally, we are supposed to mix processing and staining soloutions with equal parts bleach before disposal. Does anyone have a procedure for discarding of CSF's or other fluids? Laurie Colbert Huntington Hospital From drratanji <@t> gmail.com Thu Jan 10 12:33:06 2008 From: drratanji <@t> gmail.com (ratan choudhary) Date: Thu Jan 10 12:33:35 2008 Subject: [Histonet] immunohistochemical detection of estrogen receptor Message-ID: <6c39db40801101033i4e2577d9qb4b2fccb3510958@mail.gmail.com> Hi, I want to start detection of estrogen receptor using Qdots goat anti rabbit secondary body. I am new to use qdots. Please suggest me dilution rate and other crucial steps. Thanks Ratan On 1/10/08, histonet-request@lists.utsouthwestern.edu < histonet-request@lists.utsouthwestern.edu> wrote: > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Response to humor differences, etc. (Edwards, R.E.) > 2. Agree to disagree (Webb, Dorothy L) > 3. RE: Mart-1/Melan-A as a stain for melanoma sentinel nodes > (Dawson, Glen) > 4. Re: Who can perform Histology Duties (Rene J Buesa) > 5. Mohs Tech position in Georgia (Shirley Powell) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 10 Jan 2008 17:09:07 -0000 > From: "Edwards, R.E." > Subject: RE: [Histonet] Response to humor differences, etc. > To: "Kathleen Roberts" , > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > "Emoticons", weren't they the baddies in an early episode of Dr. > Who? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathleen > Roberts > Sent: 10 January 2008 16:59 > To: 'histonet@lists.utsouthwestern.edu' > Subject: Re: [Histonet] Response to humor differences, etc. > > Actually, in my experience, it isn't just a Histonet thing, it's an > ONLINE thing. No matter what forum or list you're on, there is always > misunderstanding, fights and flaming b/c two crucial things are missing > in online forums: tone of voice and facial expressions. (Yes, even when > those are present, misunderstandings, etc. still occur.) Emoticons > don't even begin to cut it, and sometimes when a person is reading > something, his/her mood, opinions, experience, etc. will "color" what's > being read and thus influence the person's understanding of what was > written, which the author never intended and can't control. I can't > tell you how many times that has happened to me. > > Kathleen Roberts > > Dawson, Glen wrote: > > >Humor IS shared by both Americans and British, as is misinterpretation, > misunderstanding and becoming indignant/offended over the strangest > things. > > > >It isn't an Americans vs. the British thing, it's a histonet thing. > Over-reactions are rampant and one should be aware that having a sense > of humor should have a "Use at your own risk" sign when utilizing it on > this forum. In every group there will always be a number of folks more > interested in attacking what is being said by others rather than > contributing something of their own. > > > >Each year this trend gets a bit worse & I find myself "replying to all" > less and less. Posting to an individual usually causes less of a stir > so that is the road I take much more these days. > > > >Just my .01 Euros, > > > >Glen Dawson > >IHC Manager > >Milwaukee, WI > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Kemlo > >Rogerson > >Sent: Thursday, January 10, 2008 8:44 AM > >To: Robyn Vazquez; MICHELLE.MCNEESE@childrens.com; > >histonet@lists.utsouthwestern.edu; cmiller@physlab.com; JWEEMS@sjha.org > >Subject: RE: [Histonet] Response to On-Call pay > > > > > >It's the humour Robyn, rarely travels well, I sometimes wonder if the > >difference between Americans and the British is greater than that > >between men and women. > > > > > >Kemlo Rogerson > >Pathology Manager > >DD 01934 647057 or extension 3311 > >Mob 07749 754194; Pager 07659 597107; > >Don't be afraid to take a big step when one is indicated. You can't > >cross a chasm in two small jumps. --Buckminster Fuller > > > >This e-mail is confidential and privileged. If you are not the intended > > >recipient please accept my apologies; please do not disclose, copy or > >distribute information in this e-mail or take any action in reliance on > > >its contents: to do so is strictly prohibited and may be unlawful. > >Please inform me that this message has gone astray before deleting it. > >Thank you for your co-operation > > > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 2 > Date: Thu, 10 Jan 2008 11:19:29 -0600 > From: "Webb, Dorothy L" > Subject: [Histonet] Agree to disagree > To: histonet@lists.utsouthwestern.edu > Message-ID: > <0E394B648E5284478A6CCB78E5AFDA27056353B9@hpes1.HealthPartners.int> > Content-Type: text/plain; charset="us-ascii" > > I agree with Glen inasmuch as I do not do a lot of "post" responses due > to being attacked! Constructive criticism is great and offering gentle > advice is even better, but not "putting one down" because they do not > know as much as someone else may or have a different viewpoint!! I > still LOVE the histonet and appreciate all of the great wisdom instilled > in me by those of you who keep responding to my inquiries and others!! > > Dorothy Webb, HT (ASCP) > Histology Technical Supervisor > Regions Hospital, Pathology Department > 640 Jackson Street, Saint Paul, MN 55101-2595 > Phone: 651-254-2962 > Fax: 651-254-2741 > Regions Hospital is part of the HealthPartners family of care > ________________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please be > advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is strictly > prohibited. > > If you have received this e-mail in error, please immediately notify the > HealthPartners Support Center by telephone at (952) 967-6600. You will be > reimbursed for reasonable costs incurred in notifying us. > > > ------------------------------ > > Message: 3 > Date: Thu, 10 Jan 2008 11:23:52 -0600 > From: "Dawson, Glen" > Subject: RE: [Histonet] Mart-1/Melan-A as a stain for melanoma > sentinel nodes > To: "Barnhart, Tammy" , "Histonet > \(E-mail\)" > > Message-ID: > < > B3D65550856D0146B900D401EE313D4B01274146@dynams.dynacaremilwaukee.com> > > Content-Type: text/plain; charset="iso-8859-1" > > Tammy, > > I use a cocktail of Mart-1, Melan-A & Tyrosinase that we named the MCW > (Medical College of Wisconsin) Melanoma Cocktail. If you google it you > should find our paper online and I do believe that my Pathologist addresses > some Mast cell staining issues in it. The triple antibody is excellent as a > screener as it covers more epitopes than any one of the three does > individually. Suggested staining methods are in the paper as well. > > Best of Luck, > Glen Dawson > IHC Manager > Milwaukee, WI > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Barnhart, > Tammy > Sent: Thursday, January 10, 2008 10:51 AM > To: Histonet (E-mail) > Subject: [Histonet] Mart-1/Melan-A as a stain for melanoma sentinel > nodes > > > We are having some debate about which marker to use for our melanoma > sentinel node screens. We have been using Mart-1, but recently had a case > where we think mast cells were stained. We can find no literature that > indicates that Mart-1 will stain mast cells. We use Lab Vision's > Mart-1/Melan-a AB-3 (clone M27C10+M2-9e3). We ran a Mast Cell tryptase to > see what stained, and all the cells that stained with the Mart-1 stained > plus a bunch of others. So if the Mart-1 is staining mast cells, it is > only > staining a subset of them. We really think that these are mast cells and > not melanoma, but would like to see some literature to support this. So > two > questions: 1) What are people using as a screening antibody and 2) Does > anyone have a reference that indicates that this antibody will stain mast > cells. Thanks in advance for your help. > > Tammy Barnhart, BS, HTL(ASCP) > Anatomic Pathology Supervisor > St. Alexius Medical Center > Bismarck, ND > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 4 > Date: Thu, 10 Jan 2008 09:41:38 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] Who can perform Histology Duties > To: Terri Braud , > histonet@lists.utsouthwestern.edu > Message-ID: <463239.48520.qm@web61219.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > In the same way a "well trained woo-doo priest" can perform surgery? What > about the knowledge background? Irrelevant? > Ren? J. > > Terri Braud wrote: > > > > > --------------------------------- > Looking for last minute shopping deals? Find them fast with Yahoo! > Search. > > ------------------------------ > > Message: 5 > Date: Thu, 10 Jan 2008 12:52:13 -0500 > From: Shirley Powell > Subject: [Histonet] Mohs Tech position in Georgia > To: histonet@lists.utsouthwestern.edu > Message-ID: <01MPXG4PEYQE003MTI@Macon2.Mercer.edu> > Content-Type: text/plain; charset=us-ascii > > Please Do Not reply to me. I am posting this for a friend. > Please contact the Dr. Lane or Renee Blanton at the numbers below. > > Needed immediately > Certified Mohs histotech > > Exciting new Mohs surgery practice in Columbus, Georgia > > Part-time or full-time possible > > Competitive pay > Excellent benefits > > Contact: Joshua Lane, M.D. at 706.573.6545 or Renee Blanton at > 706.322.1717 > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 50, Issue 15 > **************************************** > -- *********************************************** Ratan Kumar Choudhary Graduate Research Assistant Bovine Functional Genomics Lab Room 14 Bldg 200 BARC-East Powder Mill Road Beltsville, Maryland, 20705 USA Phone: 301-504-8342 (O) 301-931-8730 (R) Fax: (301) 504-8414 http://www.drratankumar.wetpaint.com *********************************************** From jo-ann.bader <@t> mcgill.ca Thu Jan 10 12:49:13 2008 From: jo-ann.bader <@t> mcgill.ca (Jo-Ann Bader, Ms.) Date: Thu Jan 10 12:51:32 2008 Subject: [Histonet] Lactating Mammary gland fixation Message-ID: We are proessing whole, lactating mouse mammary glands and some times the nuclei just doesn't look right, the look blurry or smeared. I am presuming it is fixation but if anyone else has more experience with lactating mammary glands I am all ears. We usually fix our tissue in 10% NBF for 24 hours at room temperature. I am planning to increase out fixation time and add agitation. If someone has other ideas or a different fixative I would be very interested in knowing. Thank You Jo-Ann Bader Histology Facility Coordinator Molecular Oncology Group MUHC/RVH 687 Pine Ave. W - Rm. M11-53 Montreal, QC, H3A-1A1 Tel: 514-934-1934 Ext: 31780 Fax: 514-843-1479 email: jo-ann.bader@mcgill.ca From mtarango <@t> nvcancer.org Thu Jan 10 13:00:47 2008 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Thu Jan 10 13:01:27 2008 Subject: [Histonet] Counterstain on Ventana stainers In-Reply-To: <3F328377AF4E23438E78234752652CE105D525F8@uwhis-xchng7.uwhis.hosp.wisc.edu> Message-ID: <5AEC610C1CE02945BD63A395BA763EDE01E48CB2@NVCIEXCH02.NVCI.org> I've used Richard-Allen's 7211 hematoxylin for a minute, and then their clarifier 2 for minute, and finally blue with buffer. That's three steps, but it looked the best compared to other things I've tried. Mark Adam Tarango HT(ASCP) Histology & IHC Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 Direct Line (702) 822-5112 Treo (702) 759-9229 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Drew Sally A. Sent: Thursday, January 10, 2008 8:30 AM To: Histonet Subject: [Histonet] Counterstain on Ventana stainers Is anyone using a non-Ventana hematoxylin counterstain on their immunostainers? If so, what works best for you in terms of intensity and stability. Just looking at a bit of cost-cutting... Thank you for your time! Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From CIngles <@t> uwhealth.org Thu Jan 10 13:09:40 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Thu Jan 10 13:12:48 2008 Subject: [Histonet] Who can perform which job functions References: Message-ID: <08A0A863637F1349BBFD83A96B27A50A1200BE@uwhis-xchng3.uwhis.hosp.wisc.edu> Actually, I just stand aside and offer them the job if they think they can do it better or faster. They usually get the hint and sneak off. I have my current pathologists trained now. :) The Fellows and Residents are another matter... Claire ...I do believe that you should be issued with a cattle prod or Taser to use liberally if the pathologist insists on looking over your shoulder while you are working! Barry From LuckG <@t> empirehealth.org Thu Jan 10 13:44:22 2008 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Thu Jan 10 13:44:48 2008 Subject: [Histonet] Who can perform Histology Duties In-Reply-To: References: <006101c853a7$14352450$3601a8c0@brownpathology.net> Message-ID: <6BB8BC4519AAB844B174FC739A679BBCCEFFAB@IRMEXCH01.irm.inhs.org> Hello All, Can anyone show me where in the CLIA regs that "they" (not CAP) distinguish between "grossing" and "processing"? Thanks, Greg -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Thursday, January 10, 2008 9:03 AM To: Bonnie Whitaker; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Who can perform Histology Duties CLIA considers "grossing" to be a high complexity test. The transference of the all tissue received in a container to a cassette, using a standard dictation is not defined as grossing, but as "processing", which is not a high complexity test. CLIA uses this clarification but it must be clearly defined in your procedure. -----Original Message----- From: Bonnie Whitaker [mailto:bwhitaker@brownpathology.com] Sent: Thursday, January 10, 2008 11:37 AM To: Terri Braud; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Who can perform Histology Duties Hi Terri, CAP has changed it's definition of grossing but most CAP labs are also required to follow CLIA, even though they aren't inspected by CLIA. Don't forget about that if you bill Medicare. They COULD come in and do an unannounced inspection and zap you if your grossers don't meet the CLIA standards for high complexity testing one way or another. Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Thursday, January 10, 2008 9:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Who can perform Histology Duties "Sharon.Davis-Devine" wrote: Ok, all of you Histonetters I have another question for you. Who the histology laboratory can perform these job functions: embedding, cutting, performing special stains and IHC? Can a lab assistant perform these duties if properly trained or do you have to be classified as a Histotech in training? Can a Cytotech or Med Tech perform such duties, again if properly trained? All opinions and references to such requirements would be greatly appreciated. Though some may not like to admit it, ANYONE properly trained and documented as competent can perform the duties listed above. The only exception is for those states that have licensure requirements for the performance of Histological duties, and then, the licensure requirements must be met. IHC is still a stain. The interpretation of that stain by the pathologist is the high complexity part of the test. Anyone properly trained and documented as competent can stain IHC. Even grossing has now been re-defined by CAP to allow submission of smaller tissues by personnel without the qualifications for High Complexity Testing (see below) (CAP checklist question and notes: ANP.11600) 1) Processing is defined as a tissue examination limited to description, inking and cutting of the specimen (if applicable), and submission of the entire specimen to histology. Tissue processing can be performed according to standardized protocols. Processing is generally limited to small specimens (skin ellipses, small biopsies, curettings, etc.) and does not require knowledge of anatomy. 2) Grossing (or gross examination) is defined as a tissue examination requiring a greater exercise of judgment and a knowledge of anatomy. Dissection of the specimen and selection of tissue samples for submission to histology are generally required. The specimen description is not necessarily standardized. Please save your flames. The scenerio of a Non-Histotech embedding, cutting, and staining my surgical tissue does not thrill me, but that was not her question. Maybe the reality of the answer is why many Histology Labs have always been considered the evil red-headed stepchildren of the Lab. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supv. Laboratory, Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3689 ------------------------------------------------------------------------ ---- ----- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ --------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Thu Jan 10 13:50:30 2008 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Thu Jan 10 13:50:56 2008 Subject: [Histonet] Who can perform Histology Duties In-Reply-To: <6BB8BC4519AAB844B174FC739A679BBCCEFFAB@IRMEXCH01.irm.inhs.org> References: <006101c853a7$14352450$3601a8c0@brownpathology.net> <6BB8BC4519AAB844B174FC739A679BBCCEFFAB@IRMEXCH01.irm.inhs.org> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F487B@sjhaexc02.sjha.org> That's in the new CAP regs.. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Luck, Greg D. Sent: Thursday, January 10, 2008 2:44 PM To: Terri Braud; Bonnie Whitaker; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Who can perform Histology Duties Hello All, Can anyone show me where in the CLIA regs that "they" (not CAP) distinguish between "grossing" and "processing"? Thanks, Greg -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Thursday, January 10, 2008 9:03 AM To: Bonnie Whitaker; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Who can perform Histology Duties CLIA considers "grossing" to be a high complexity test. The transference of the all tissue received in a container to a cassette, using a standard dictation is not defined as grossing, but as "processing", which is not a high complexity test. CLIA uses this clarification but it must be clearly defined in your procedure. -----Original Message----- From: Bonnie Whitaker [mailto:bwhitaker@brownpathology.com] Sent: Thursday, January 10, 2008 11:37 AM To: Terri Braud; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Who can perform Histology Duties Hi Terri, CAP has changed it's definition of grossing but most CAP labs are also required to follow CLIA, even though they aren't inspected by CLIA. Don't forget about that if you bill Medicare. They COULD come in and do an unannounced inspection and zap you if your grossers don't meet the CLIA standards for high complexity testing one way or another. Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Thursday, January 10, 2008 9:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Who can perform Histology Duties "Sharon.Davis-Devine" wrote: Ok, all of you Histonetters I have another question for you. Who the histology laboratory can perform these job functions: embedding, cutting, performing special stains and IHC? Can a lab assistant perform these duties if properly trained or do you have to be classified as a Histotech in training? Can a Cytotech or Med Tech perform such duties, again if properly trained? All opinions and references to such requirements would be greatly appreciated. Though some may not like to admit it, ANYONE properly trained and documented as competent can perform the duties listed above. The only exception is for those states that have licensure requirements for the performance of Histological duties, and then, the licensure requirements must be met. IHC is still a stain. The interpretation of that stain by the pathologist is the high complexity part of the test. Anyone properly trained and documented as competent can stain IHC. Even grossing has now been re-defined by CAP to allow submission of smaller tissues by personnel without the qualifications for High Complexity Testing (see below) (CAP checklist question and notes: ANP.11600) 1) Processing is defined as a tissue examination limited to description, inking and cutting of the specimen (if applicable), and submission of the entire specimen to histology. Tissue processing can be performed according to standardized protocols. Processing is generally limited to small specimens (skin ellipses, small biopsies, curettings, etc.) and does not require knowledge of anatomy. 2) Grossing (or gross examination) is defined as a tissue examination requiring a greater exercise of judgment and a knowledge of anatomy. Dissection of the specimen and selection of tissue samples for submission to histology are generally required. The specimen description is not necessarily standardized. Please save your flames. The scenerio of a Non-Histotech embedding, cutting, and staining my surgical tissue does not thrill me, but that was not her question. Maybe the reality of the answer is why many Histology Labs have always been considered the evil red-headed stepchildren of the Lab. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supv. Laboratory, Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3689 ------------------------------------------------------------------------ ---- ----- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ --------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Thu Jan 10 14:21:03 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Jan 10 14:21:31 2008 Subject: AW: [Histonet] Counterstain on Ventana stainers In-Reply-To: <3F328377AF4E23438E78234752652CE105D525F8@uwhis-xchng7.uwhis.hosp.wisc.edu> Message-ID: <000601c853c6$537cb190$eeeea8c0@dielangs.at> We use our house-made Mayer's haemalaun 1:1 diluted with A.d. For bluing we use the Ventana-reagens. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Drew Sally A. Gesendet: Donnerstag, 10. J?nner 2008 17:30 An: Histonet Betreff: [Histonet] Counterstain on Ventana stainers Is anyone using a non-Ventana hematoxylin counterstain on their immunostainers? If so, what works best for you in terms of intensity and stability. Just looking at a bit of cost-cutting... Thank you for your time! Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Thu Jan 10 14:24:24 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Jan 10 14:24:48 2008 Subject: AW: [Histonet] Mart-1/Melan-A as a stain for melanoma sentinel nodes In-Reply-To: <4F0B7161A6CD524FAD8017D52E1553407AE610@exchangent> Message-ID: <000701c853c6$ca801200$eeeea8c0@dielangs.at> We use the Panmelanoma-Cocktail from Biocare. http://www.nordiqc.org/Run-20-B3/Assessment/assessment-MLA.htm This links shows the information of Nordiqc about this epitope. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Barnhart, Tammy Gesendet: Donnerstag, 10. J?nner 2008 17:51 An: Histonet (E-mail) Betreff: [Histonet] Mart-1/Melan-A as a stain for melanoma sentinel nodes We are having some debate about which marker to use for our melanoma sentinel node screens. We have been using Mart-1, but recently had a case where we think mast cells were stained. We can find no literature that indicates that Mart-1 will stain mast cells. We use Lab Vision's Mart-1/Melan-a AB-3 (clone M27C10+M2-9e3). We ran a Mast Cell tryptase to see what stained, and all the cells that stained with the Mart-1 stained plus a bunch of others. So if the Mart-1 is staining mast cells, it is only staining a subset of them. We really think that these are mast cells and not melanoma, but would like to see some literature to support this. So two questions: 1) What are people using as a screening antibody and 2) Does anyone have a reference that indicates that this antibody will stain mast cells. Thanks in advance for your help. Tammy Barnhart, BS, HTL(ASCP) Anatomic Pathology Supervisor St. Alexius Medical Center Bismarck, ND _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud <@t> holyredeemer.com Thu Jan 10 14:36:12 2008 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Thu Jan 10 14:36:34 2008 Subject: [Histonet] Who can perform Histology Duties In-Reply-To: <6BB8BC4519AAB844B174FC739A679BBCCEFFAB@IRMEXCH01.irm.inhs.org> Message-ID: To answer your question: That authority, granted by the Health Care Financing Administration (HCFA) last June, recognizes CAP as having fulfilled "all the requirements necessary to be granted deemed status under CLIA." "Deemed status" means if you meet the CAP requirements, you will meet CLIA's I hope that finally clarifies the answer for everyone. Terri -----Original Message----- From: Luck, Greg D. [mailto:LuckG@empirehealth.org] Sent: Thursday, January 10, 2008 2:44 PM To: Terri Braud; Bonnie Whitaker; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Who can perform Histology Duties Hello All, Can anyone show me where in the CLIA regs that "they" (not CAP) distinguish between "grossing" and "processing"? Thanks, Greg -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Thursday, January 10, 2008 9:03 AM To: Bonnie Whitaker; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Who can perform Histology Duties CLIA considers "grossing" to be a high complexity test. The transference of the all tissue received in a container to a cassette, using a standard dictation is not defined as grossing, but as "processing", which is not a high complexity test. CLIA uses this clarification but it must be clearly defined in your procedure. --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From JWEEMS <@t> sjha.org Thu Jan 10 14:58:27 2008 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Thu Jan 10 14:58:52 2008 Subject: [Histonet] Who can perform Histology Duties In-Reply-To: References: <6BB8BC4519AAB844B174FC739A679BBCCEFFAB@IRMEXCH01.irm.inhs.org> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F487F@sjhaexc02.sjha.org> Well, thank you Terri!!! I am saving this for documentation. I had a procedure called "Biopsy Grossing" which explained what was in that category and had a competency checklist to be signed by a pathologist. I was dinged by the CAP inspector for calling it grossing - so I poured over the CAP regs and found the distinction but had not connected the two. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Thursday, January 10, 2008 3:36 PM To: Luck, Greg D.; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Who can perform Histology Duties To answer your question: That authority, granted by the Health Care Financing Administration (HCFA) last June, recognizes CAP as having fulfilled "all the requirements necessary to be granted deemed status under CLIA." "Deemed status" means if you meet the CAP requirements, you will meet CLIA's I hope that finally clarifies the answer for everyone. Terri -----Original Message----- From: Luck, Greg D. [mailto:LuckG@empirehealth.org] Sent: Thursday, January 10, 2008 2:44 PM To: Terri Braud; Bonnie Whitaker; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Who can perform Histology Duties Hello All, Can anyone show me where in the CLIA regs that "they" (not CAP) distinguish between "grossing" and "processing"? Thanks, Greg -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Thursday, January 10, 2008 9:03 AM To: Bonnie Whitaker; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Who can perform Histology Duties CLIA considers "grossing" to be a high complexity test. The transference of the all tissue received in a container to a cassette, using a standard dictation is not defined as grossing, but as "processing", which is not a high complexity test. CLIA uses this clarification but it must be clearly defined in your procedure. ------------------------------------------------------------------------ --------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wfubmc.edu Thu Jan 10 15:26:14 2008 From: mward <@t> wfubmc.edu (Martha Ward) Date: Thu Jan 10 15:26:38 2008 Subject: [Histonet] biomeda corporation Message-ID: <61135F0455D33347B5AAE209B903A30420170589@EXCHVS2.medctr.ad.wfubmc.edu> I have been trying to reach Biomeda Corp. The website gives numbers to call or fax orders but we can't get through. Anybody know what is going on????? Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center Medical Center Blvd. Winston-Salem, NC 27157 336-716-2756 mward@wfubmc.edu From gayle.callis <@t> bresnan.net Thu Jan 10 15:38:13 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Thu Jan 10 15:38:41 2008 Subject: [Histonet] immunohistochemical detection of estrogen receptor References: <6c39db40801101033i4e2577d9qb4b2fccb3510958@mail.gmail.com> Message-ID: <005101c853d1$1aafd3a0$6501a8c0@DHXTS541> QDot website used to give protocols on how to work with these nanoparticles. Also, in publications on QDots for estrogen receptors, you may be able to dig out info from their methods and materials. Try PUBMED or Goggle Scholar search. Gayle M. Callis HT/HTL/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "ratan choudhary" To: Sent: Thursday, January 10, 2008 11:33 AM Subject: [Histonet] immunohistochemical detection of estrogen receptor Hi, I want to start detection of estrogen receptor using Qdots goat anti rabbit secondary body. I am new to use qdots. Please suggest me dilution rate and other crucial steps. Thanks Ratan On 1/10/08, histonet-request@lists.utsouthwestern.edu < histonet-request@lists.utsouthwestern.edu> wrote: > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Response to humor differences, etc. (Edwards, R.E.) > 2. Agree to disagree (Webb, Dorothy L) > 3. RE: Mart-1/Melan-A as a stain for melanoma sentinel nodes > (Dawson, Glen) > 4. Re: Who can perform Histology Duties (Rene J Buesa) > 5. Mohs Tech position in Georgia (Shirley Powell) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 10 Jan 2008 17:09:07 -0000 > From: "Edwards, R.E." > Subject: RE: [Histonet] Response to humor differences, etc. > To: "Kathleen Roberts" , > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > "Emoticons", weren't they the baddies in an early episode of Dr. > Who? > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathleen > Roberts > Sent: 10 January 2008 16:59 > To: 'histonet@lists.utsouthwestern.edu' > Subject: Re: [Histonet] Response to humor differences, etc. > > Actually, in my experience, it isn't just a Histonet thing, it's an > ONLINE thing. No matter what forum or list you're on, there is always > misunderstanding, fights and flaming b/c two crucial things are missing > in online forums: tone of voice and facial expressions. (Yes, even when > those are present, misunderstandings, etc. still occur.) Emoticons > don't even begin to cut it, and sometimes when a person is reading > something, his/her mood, opinions, experience, etc. will "color" what's > being read and thus influence the person's understanding of what was > written, which the author never intended and can't control. I can't > tell you how many times that has happened to me. > > Kathleen Roberts > > Dawson, Glen wrote: > > >Humor IS shared by both Americans and British, as is misinterpretation, > misunderstanding and becoming indignant/offended over the strangest > things. > > > >It isn't an Americans vs. the British thing, it's a histonet thing. > Over-reactions are rampant and one should be aware that having a sense > of humor should have a "Use at your own risk" sign when utilizing it on > this forum. In every group there will always be a number of folks more > interested in attacking what is being said by others rather than > contributing something of their own. > > > >Each year this trend gets a bit worse & I find myself "replying to all" > less and less. Posting to an individual usually causes less of a stir > so that is the road I take much more these days. > > > >Just my .01 Euros, > > > >Glen Dawson > >IHC Manager > >Milwaukee, WI > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Kemlo > >Rogerson > >Sent: Thursday, January 10, 2008 8:44 AM > >To: Robyn Vazquez; MICHELLE.MCNEESE@childrens.com; > >histonet@lists.utsouthwestern.edu; cmiller@physlab.com; JWEEMS@sjha.org > >Subject: RE: [Histonet] Response to On-Call pay > > > > > >It's the humour Robyn, rarely travels well, I sometimes wonder if the > >difference between Americans and the British is greater than that > >between men and women. > > > > > >Kemlo Rogerson > >Pathology Manager > >DD 01934 647057 or extension 3311 > >Mob 07749 754194; Pager 07659 597107; > >Don't be afraid to take a big step when one is indicated. You can't > >cross a chasm in two small jumps. --Buckminster Fuller > > > >This e-mail is confidential and privileged. If you are not the intended > > >recipient please accept my apologies; please do not disclose, copy or > >distribute information in this e-mail or take any action in reliance on > > >its contents: to do so is strictly prohibited and may be unlawful. > >Please inform me that this message has gone astray before deleting it. > >Thank you for your co-operation > > > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 2 > Date: Thu, 10 Jan 2008 11:19:29 -0600 > From: "Webb, Dorothy L" > Subject: [Histonet] Agree to disagree > To: histonet@lists.utsouthwestern.edu > Message-ID: > <0E394B648E5284478A6CCB78E5AFDA27056353B9@hpes1.HealthPartners.int> > Content-Type: text/plain; charset="us-ascii" > > I agree with Glen inasmuch as I do not do a lot of "post" responses due > to being attacked! Constructive criticism is great and offering gentle > advice is even better, but not "putting one down" because they do not > know as much as someone else may or have a different viewpoint!! I > still LOVE the histonet and appreciate all of the great wisdom instilled > in me by those of you who keep responding to my inquiries and others!! > > Dorothy Webb, HT (ASCP) > Histology Technical Supervisor > Regions Hospital, Pathology Department > 640 Jackson Street, Saint Paul, MN 55101-2595 > Phone: 651-254-2962 > Fax: 651-254-2741 > Regions Hospital is part of the HealthPartners family of care > ________________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please be > advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is strictly > prohibited. > > If you have received this e-mail in error, please immediately notify the > HealthPartners Support Center by telephone at (952) 967-6600. You will be > reimbursed for reasonable costs incurred in notifying us. > > > ------------------------------ > > Message: 3 > Date: Thu, 10 Jan 2008 11:23:52 -0600 > From: "Dawson, Glen" > Subject: RE: [Histonet] Mart-1/Melan-A as a stain for melanoma > sentinel nodes > To: "Barnhart, Tammy" , "Histonet > \(E-mail\)" > > Message-ID: > < > B3D65550856D0146B900D401EE313D4B01274146@dynams.dynacaremilwaukee.com> > > Content-Type: text/plain; charset="iso-8859-1" > > Tammy, > > I use a cocktail of Mart-1, Melan-A & Tyrosinase that we named the MCW > (Medical College of Wisconsin) Melanoma Cocktail. If you google it you > should find our paper online and I do believe that my Pathologist > addresses > some Mast cell staining issues in it. The triple antibody is excellent as > a > screener as it covers more epitopes than any one of the three does > individually. Suggested staining methods are in the paper as well. > > Best of Luck, > Glen Dawson > IHC Manager > Milwaukee, WI > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Barnhart, > Tammy > Sent: Thursday, January 10, 2008 10:51 AM > To: Histonet (E-mail) > Subject: [Histonet] Mart-1/Melan-A as a stain for melanoma sentinel > nodes > > > We are having some debate about which marker to use for our melanoma > sentinel node screens. We have been using Mart-1, but recently had a case > where we think mast cells were stained. We can find no literature that > indicates that Mart-1 will stain mast cells. We use Lab Vision's > Mart-1/Melan-a AB-3 (clone M27C10+M2-9e3). We ran a Mast Cell tryptase to > see what stained, and all the cells that stained with the Mart-1 stained > plus a bunch of others. So if the Mart-1 is staining mast cells, it is > only > staining a subset of them. We really think that these are mast cells and > not melanoma, but would like to see some literature to support this. So > two > questions: 1) What are people using as a screening antibody and 2) Does > anyone have a reference that indicates that this antibody will stain mast > cells. Thanks in advance for your help. > > Tammy Barnhart, BS, HTL(ASCP) > Anatomic Pathology Supervisor > St. Alexius Medical Center > Bismarck, ND > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 4 > Date: Thu, 10 Jan 2008 09:41:38 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] Who can perform Histology Duties > To: Terri Braud , > histonet@lists.utsouthwestern.edu > Message-ID: <463239.48520.qm@web61219.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > In the same way a "well trained woo-doo priest" can perform surgery? What > about the knowledge background? Irrelevant? > Ren? J. > > Terri Braud wrote: > > > > > --------------------------------- > Looking for last minute shopping deals? Find them fast with Yahoo! > Search. > > ------------------------------ > > Message: 5 > Date: Thu, 10 Jan 2008 12:52:13 -0500 > From: Shirley Powell > Subject: [Histonet] Mohs Tech position in Georgia > To: histonet@lists.utsouthwestern.edu > Message-ID: <01MPXG4PEYQE003MTI@Macon2.Mercer.edu> > Content-Type: text/plain; charset=us-ascii > > Please Do Not reply to me. I am posting this for a friend. > Please contact the Dr. Lane or Renee Blanton at the numbers below. > > Needed immediately > Certified Mohs histotech > > Exciting new Mohs surgery practice in Columbus, Georgia > > Part-time or full-time possible > > Competitive pay > Excellent benefits > > Contact: Joshua Lane, M.D. at 706.573.6545 or Renee Blanton at > 706.322.1717 > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 50, Issue 15 > **************************************** > -- *********************************************** Ratan Kumar Choudhary Graduate Research Assistant Bovine Functional Genomics Lab Room 14 Bldg 200 BARC-East Powder Mill Road Beltsville, Maryland, 20705 USA Phone: 301-504-8342 (O) 301-931-8730 (R) Fax: (301) 504-8414 http://www.drratankumar.wetpaint.com *********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rfields <@t> gidocs.net Thu Jan 10 16:01:28 2008 From: rfields <@t> gidocs.net (Rosa Fields) Date: Thu Jan 10 16:01:50 2008 Subject: [Histonet] microwave processors Message-ID: <2F2611250DCD6549AA3D96CE8AF1F018DAD537@giexchange.gidocs.net> anyone had experience with the SHUR/Wave or Shandon TissueWave processors? The good, the bad or the ulgy? Rosa Fields, HT (ASCP) Histology Supervisor Gastroenterology Specialties P.C. 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net From babears <@t> triad.rr.com Thu Jan 10 16:11:56 2008 From: babears <@t> triad.rr.com (Pete and Sharon Jack) Date: Thu Jan 10 16:12:18 2008 Subject: [Histonet] Leprosy control block Message-ID: <000b01c853d5$d0319930$90e84c47@pete38d56c4d11> Does anyone know where I can purchase a control block for leprosy for our Fite stain? Sharon S. Jack, HT(ASCP) From JMacDonald <@t> mtsac.edu Thu Jan 10 16:56:16 2008 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Jan 10 16:56:50 2008 Subject: [Histonet] California Society for Histotechnology call for abstracts Message-ID: The California Society for Histotechnology will be holding its annual symposium May 15-18, 2008 in Ventura. We are looking for workshops. Please let me know if you would be interested in presenting. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu From Warren_Eddings <@t> ssmhc.com Thu Jan 10 17:15:27 2008 From: Warren_Eddings <@t> ssmhc.com (Warren_Eddings@ssmhc.com) Date: Thu Jan 10 17:16:32 2008 Subject: [Histonet] Fw: antibody validation Message-ID: -----Forwarded by Warren Eddings/ ----- To: histonet@lists.utsouthwestern.edu From: Warren Eddings/SAHOKC/SSMHC Date: 01/08/2008 05:33PM Subjec hello does anyone have a procedure for the above thanks warren_eddings@ssmhc.com _________________________________________________________________ Confidentiality Noti attachments, is for the sole use of t may contain confidential and privileged inform unauthorized review, use, disclosure or distribution is prohib ited. If you are not the intended recipient, please contact the sender by message. From judi.ford <@t> roche.com Thu Jan 10 17:18:53 2008 From: judi.ford <@t> roche.com (Ford, Judi) Date: Thu Jan 10 17:19:20 2008 Subject: [Histonet] Tissue marking dyes and IHC Message-ID: Hi all, I was just curious to find out if tissue marked with either hematoxylin and/or eosin, or tissue marking dyes, can that affect future IHC and/or immunofluorescent staining? We have a situation where a right and left piece of tissue will be embedded on one block and we want to distinguish the two pieces. They are very small DRG's which can get lost once embedded. One thought was to use dyes to mark them, but I was concerned about the possibility of interference with immuno staining. I'd love to hear your thoughts. Thanks soooo much! Judi Ford, BA, HTL(ASCP) Research Associate III Roche Palo Alto 3431 Hillview Ave Palo Alto, CA 94304 From YuJ2 <@t> upmc.edu Thu Jan 10 17:32:42 2008 From: YuJ2 <@t> upmc.edu (Yu, Jian) Date: Thu Jan 10 17:33:04 2008 Subject: [Histonet] Paneth cell dyes or markers In-Reply-To: References: <6BB8BC4519AAB844B174FC739A679BBCCEFFAB@IRMEXCH01.irm.inhs.org> Message-ID: <7E0A77BFEB9A1E47A63F978E7116821F07986F70@1upmc-msx11.acct.upmchs.net> Does anyone know a good Paneth cell specific dye or IHC marker that works on paraffin sections of mouse small intestine? Thanks a lot! Jian Yu, Ph.D. University of Pittsburgh Cancer Institute Hillman Cancer Center Research Pavilion Office Suite 2.26h, Laboratory 2.43 5117 Centre Avenue, Pittsburgh, PA 15213 Phone: 412-623-7786, (Lab) 412-623-3255 Fax: 412-623-7778 Email: yuj2@upmc.edu From ttruscot <@t> vetmed.wsu.edu Thu Jan 10 17:34:33 2008 From: ttruscot <@t> vetmed.wsu.edu (Truscott, Tom) Date: Thu Jan 10 17:35:03 2008 Subject: [Histonet] Tissue marking dyes and IHC In-Reply-To: References: Message-ID: <35CF12B690D8CA4E95375A36B4E7B44CB6BDD2@cvm36.vetmed.wsu.edu> Hi Judi, I have not tested the hypothesis, but I worked for a pathologist who would not let us use anything but black India ink on any tissue that was to be tested with ER/PR IHC because plant dyes may contain their own estrogens. Tom Truscott USDA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ford, Judi Sent: Thursday, January 10, 2008 3:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue marking dyes and IHC Hi all, I was just curious to find out if tissue marked with either hematoxylin and/or eosin, or tissue marking dyes, can that affect future IHC and/or immunofluorescent staining? We have a situation where a right and left piece of tissue will be embedded on one block and we want to distinguish the two pieces. They are very small DRG's which can get lost once embedded. One thought was to use dyes to mark them, but I was concerned about the possibility of interference with immuno staining. I'd love to hear your thoughts. Thanks soooo much! Judi Ford, BA, HTL(ASCP) Research Associate III Roche Palo Alto 3431 Hillview Ave Palo Alto, CA 94304 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rosenfeldtek <@t> hotmail.com Thu Jan 10 17:41:48 2008 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Thu Jan 10 17:42:12 2008 Subject: [Histonet] Tissue marking dyes and IHC In-Reply-To: <35CF12B690D8CA4E95375A36B4E7B44CB6BDD2@cvm36.vetmed.wsu.edu> References: <35CF12B690D8CA4E95375A36B4E7B44CB6BDD2@cvm36.vetmed.wsu.edu> Message-ID: I have had very good luck with IHC on formalin-fixed, paraffin embedded tissue that was previously stained with eosin. The eosin comes out when I deparaffinize and rehydrate the slides. Jerry Ricks Research Scientist University of Washington Department of Pathology > Date: Thu, 10 Jan 2008 15:34:33 -0800> From: ttruscot@vetmed.wsu.edu> To: judi.ford@roche.com; histonet@lists.utsouthwestern.edu> Subject: RE: [Histonet] Tissue marking dyes and IHC> CC: > > Hi Judi, I have not tested the hypothesis, but I worked for a> pathologist who would not let us use anything but black India ink on any> tissue that was to be tested with ER/PR IHC because plant dyes may> contain their own estrogens. Tom Truscott USDA> > -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ford,> Judi> Sent: Thursday, January 10, 2008 3:19 PM> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] Tissue marking dyes and IHC> > Hi all,> > I was just curious to find out if tissue marked with either hematoxylin> and/or eosin, or tissue marking dyes, can that affect future IHC and/or> immunofluorescent staining? We have a situation where a right and left> piece of tissue will be embedded on one block and we want to distinguish> the two pieces. They are very small DRG's which can get lost once> embedded. One thought was to use dyes to mark them, but I was concerned> about the possibility of interference with immuno staining.> > I'd love to hear your thoughts.> > Thanks soooo much! > > Judi Ford, BA, HTL(ASCP)> Research Associate III> Roche Palo Alto> 3431 Hillview Ave> Palo Alto, CA 94304> > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Watch ?Cause Effect,? a show about real people making a real difference. http://im.live.com/Messenger/IM/MTV/?source=text_watchcause From LRaff <@t> lab.uropartners.com Thu Jan 10 20:45:39 2008 From: LRaff <@t> lab.uropartners.com (Lester Raff) Date: Thu Jan 10 20:47:29 2008 Subject: [Histonet] Tissue marking dyes and IHC References: Message-ID: <5DA1CA5D0B98A84985B545A24423B8220A9B96@UPLAB01.uplab.local> Hi: We use the Davidson (sp) tissue marking dye on all our prostate biopsies and do not have any problems with our immunos. We do not use the red dye though, because we are afraid it might be confused with AMACR staining on the PIN4 cocktail. Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory ph: 708-486-0076 fax: 708-486-0080 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Ford, Judi Sent: Thu 1/10/2008 5:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue marking dyes and IHC Hi all, I was just curious to find out if tissue marked with either hematoxylin and/or eosin, or tissue marking dyes, can that affect future IHC and/or immunofluorescent staining? We have a situation where a right and left piece of tissue will be embedded on one block and we want to distinguish the two pieces. They are very small DRG's which can get lost once embedded. One thought was to use dyes to mark them, but I was concerned about the possibility of interference with immuno staining. I'd love to hear your thoughts. Thanks soooo much! Judi Ford, BA, HTL(ASCP) Research Associate III Roche Palo Alto 3431 Hillview Ave Palo Alto, CA 94304 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Jan 10 21:15:19 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Jan 10 21:15:32 2008 Subject: [Histonet] Response to humor differences, etc. References: <47864EE3.5010009@rci.rutgers.edu> Message-ID: <006c01c85400$328e9f40$0302a8c0@yourxhtr8hvc4p> moody? Who's moody? I'm not moody! Who said that? I'm not misunderstood. I'm misinterpreted. I'm not hated. I'm unloved. We're not going down that British-American road thing again are we? I mean ,didn't we settle our differences in 1776 and again in 1812? Isn't this how wars start? Not that I want another war. Am I rambling? Geez, is it Friday yet? JTT ----- Original Message ----- From: "Kathleen Roberts" To: Sent: Thursday, January 10, 2008 10:59 AM Subject: Re: [Histonet] Response to humor differences, etc. > Actually, in my experience, it isn't just a Histonet thing, it's an ONLINE > thing. No matter what forum or list you're on, there is always > misunderstanding, fights and flaming b/c two crucial things are missing in > online forums: tone of voice and facial expressions. (Yes, even when those > are present, misunderstandings, etc. still occur.) Emoticons don't even > begin to cut it, and sometimes when a person is reading something, his/her > mood, opinions, experience, etc. will "color" what's being read and thus > influence the person's understanding of what was written, which the author > never intended and can't control. I can't tell you how many times that > has happened to me. > Kathleen Roberts > > Dawson, Glen wrote: > >>Humor IS shared by both Americans and British, as is misinterpretation, >>misunderstanding and becoming indignant/offended over the strangest >>things. >>It isn't an Americans vs. the British thing, it's a histonet thing. >>Over-reactions are rampant and one should be aware that having a sense of >>humor should have a "Use at your own risk" sign when utilizing it on this >>forum. In every group there will always be a number of folks more >>interested in attacking what is being said by others rather than >>contributing something of their own. >>Each year this trend gets a bit worse & I find myself "replying to all" >>less and less. Posting to an individual usually causes less of a stir so >>that is the road I take much more these days. >> >>Just my .01 Euros, >> >>Glen Dawson >>IHC Manager >>Milwaukee, WI >> >>-----Original Message----- >>From: histonet-bounces@lists.utsouthwestern.edu >>[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Kemlo >>Rogerson >>Sent: Thursday, January 10, 2008 8:44 AM >>To: Robyn Vazquez; MICHELLE.MCNEESE@childrens.com; >>histonet@lists.utsouthwestern.edu; cmiller@physlab.com; JWEEMS@sjha.org >>Subject: RE: [Histonet] Response to On-Call pay >> >> >>It's the humour Robyn, rarely travels well, I sometimes wonder if the >>difference between Americans and the British is greater than that >>between men and women. >> Kemlo Rogerson Pathology Manager >>DD 01934 647057 or extension 3311 >>Mob 07749 754194; Pager 07659 597107; >>Don't be afraid to take a big step when one is indicated. You can't >>cross a chasm in two small jumps. --Buckminster Fuller >>This e-mail is confidential and privileged. If you are not the intended >>recipient please accept my apologies; please do not disclose, copy or >>distribute information in this e-mail or take any action in reliance on >>its contents: to do so is strictly prohibited and may be unlawful. >>Please inform me that this message has gone astray before deleting it. >>Thank you for your co-operation >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Jan 10 21:23:50 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Jan 10 21:24:07 2008 Subject: [Histonet] Agree to disagree References: <0E394B648E5284478A6CCB78E5AFDA27056353B9@hpes1.HealthPartners.int> Message-ID: <008201c85401$62ff2d10$0302a8c0@yourxhtr8hvc4p> I can sympathize with you Dorothy. Unfortunately, because of this reluctance to reply openly, others suffer because of the private replies. Please don't get me wrong. I'm not blaming you, I've done the same thing several times, but you are right. Sometimes it's not worth the hassle. On the other hand, sometimes I look forward to the hassle. I mean, how long was I off the Histonet? I can not stand any one who belittles or looks down on some one else because they lack experience or knowledge. Don't we get that enough from the pathologists? We all must remember that at some point, and it might have been many moons ago, that we, did not have the experience or the knowledge that we have today. Can you imagine how much knowledge is spread because of the Histonet? Can you imagine what could have been if the Histonet was in existence 30-40 years ago? Joe ----- Original Message ----- From: "Webb, Dorothy L" To: Sent: Thursday, January 10, 2008 11:19 AM Subject: [Histonet] Agree to disagree I agree with Glen inasmuch as I do not do a lot of "post" responses due to being attacked! Constructive criticism is great and offering gentle advice is even better, but not "putting one down" because they do not know as much as someone else may or have a different viewpoint!! I still LOVE the histonet and appreciate all of the great wisdom instilled in me by those of you who keep responding to my inquiries and others!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Jan 10 21:26:19 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Jan 10 21:26:36 2008 Subject: [Histonet] biomeda corporation References: <61135F0455D33347B5AAE209B903A30420170589@EXCHVS2.medctr.ad.wfubmc.edu> Message-ID: <00b601c85401$bbcec590$0302a8c0@yourxhtr8hvc4p> Martha, have you tried contacting your local ThermoShandonFisher, etc, etc, rep? I know that Fisher was a distributor of Biomeda products. JTT ----- Original Message ----- From: "Martha Ward" To: Sent: Thursday, January 10, 2008 3:26 PM Subject: [Histonet] biomeda corporation I have been trying to reach Biomeda Corp. The website gives numbers to call or fax orders but we can't get through. Anybody know what is going on????? Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center Medical Center Blvd. Winston-Salem, NC 27157 336-716-2756 mward@wfubmc.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barry.R.Rittman <@t> uth.tmc.edu Thu Jan 10 22:04:07 2008 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Thu Jan 10 22:05:02 2008 Subject: [Histonet] Paneth cell dyes or markers References: <6BB8BC4519AAB844B174FC739A679BBCCEFFAB@IRMEXCH01.irm.inhs.org> <7E0A77BFEB9A1E47A63F978E7116821F07986F70@1upmc-msx11.acct.upmchs.net> Message-ID: Lendrums phloxine tartrazine stain. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Yu, Jian Sent: Thu 1/10/2008 5:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paneth cell dyes or markers Does anyone know a good Paneth cell specific dye or IHC marker that works on paraffin sections of mouse small intestine? Thanks a lot! Jian Yu, Ph.D. University of Pittsburgh Cancer Institute Hillman Cancer Center Research Pavilion Office Suite 2.26h, Laboratory 2.43 5117 Centre Avenue, Pittsburgh, PA 15213 Phone: 412-623-7786, (Lab) 412-623-3255 Fax: 412-623-7778 Email: yuj2@upmc.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NHeath <@t> Lifespan.org Fri Jan 11 05:15:32 2008 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Fri Jan 11 05:16:03 2008 Subject: [Histonet] Journal Of Histotechnology December 2007 issue Message-ID: <130E8991F210424096EFC6F42EA33B2401D743A4@LSCOEXCH1.lsmaster.lifespan.org> Hi, Does anyone out there in histo land know where I can get a copy of the December 2007 Journal Of Histotechnology??? I've emailed NSH a few times with no response. Yes I know...I know...I'm renewing my membership. Thanks, Nancy From rjbuesa <@t> yahoo.com Fri Jan 11 07:25:24 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 11 07:25:47 2008 Subject: [Histonet] Leprosy control block In-Reply-To: <000b01c853d5$d0319930$90e84c47@pete38d56c4d11> Message-ID: <488417.82293.qm@web61220.mail.yahoo.com> Fite stain also is used for the much more common TB mycobacterium. Do you need specifically leprosy mycobacterium? Ren? J. Pete and Sharon Jack wrote: --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From rjbuesa <@t> yahoo.com Fri Jan 11 07:27:32 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 11 07:27:55 2008 Subject: [Histonet] Tissue marking dyes and IHC In-Reply-To: Message-ID: <458372.45005.qm@web61225.mail.yahoo.com> I always dyed (with eosin) very small biopsies without any interference with following IHC procedures. Ren? J. "Ford, Judi" wrote: --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From ROrr <@t> enh.org Fri Jan 11 07:59:17 2008 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Fri Jan 11 07:59:42 2008 Subject: [Histonet] antibody validation Message-ID: HI Warren, I have a validation protocol, it' s pretty general, though, I'd like to send it to you, but I can't get this address to cut and paste, nor can I send it when I type it in...strange! Maybe email me separately and I'll be able to attach the file. Becky rorr@enh.org Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 Message: 19 Date: Thu, 10 Jan 2008 17:15:27 -0600 From: Warren_Eddings@ssmhc.com Subject: [Histonet] Fw: antibody validation To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="ISO-8859-1" -----Forwarded by Warren Eddings/ ----- To: histonet@lists.utsouthwestern.edu From: Warren Eddings/SAHOKC/SSMHC Date: 01/08/2008 05:33PM Subjec hello does anyone have a procedure for the above thanks warren_eddings@ssmhc.com _________________________________________________________________ Confidentiality Noti attachments, is for the sole use of t may contain confidential and privileged inform unauthorized review, use, disclosure or distribution is prohib ited. If you are not the intended recipient, please contact the sender by message. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 50, Issue 16 **************************************** From fudo <@t> ufl.edu Fri Jan 11 08:21:44 2008 From: fudo <@t> ufl.edu (FU,DONGTAO) Date: Fri Jan 11 08:22:07 2008 Subject: [Histonet] lipoma fixation(for paraffin embedding) Message-ID: <1910033357.221321200061304449.JavaMail.osg@osgjas04.cns.ufl.edu> Hi, all We will harvest lipomas and was asked to fix them in acid formalin. Does anyone have experiences on it? How to make acid formalin? or where to buy it? How long should I fix them? If I use 10%NBF instead of acid formalin, how long should I fix them? Many thanks, Ann Dongtao Fu MD, Ph.D Lab Manager Dept. of Pathology Lab phone: 352-273-7752 Lab FAX: 352-273-7755 Lab address: D11-50 PO Box: 100275 1600 SW Archer Road University of Flodrida Gainesville, FL 32610 From cmiller <@t> physlab.com Fri Jan 11 08:48:25 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Fri Jan 11 08:48:14 2008 Subject: [Histonet] Agree to disagree In-Reply-To: <008201c85401$62ff2d10$0302a8c0@yourxhtr8hvc4p> References: <0E394B648E5284478A6CCB78E5AFDA27056353B9@hpes1.HealthPartners.int> <008201c85401$62ff2d10$0302a8c0@yourxhtr8hvc4p> Message-ID: <004901c85461$05550d00$3d02a8c0@plab.local> Very well said Joe, We get kicked around enough by pathologists etc. Our peers should be supportave! I for one am happy you still grace us with your wisdom and wit!.....Cheri -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, January 10, 2008 9:24 PM To: Webb, Dorothy L; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Agree to disagree I can sympathize with you Dorothy. Unfortunately, because of this reluctance to reply openly, others suffer because of the private replies. Please don't get me wrong. I'm not blaming you, I've done the same thing several times, but you are right. Sometimes it's not worth the hassle. On the other hand, sometimes I look forward to the hassle. I mean, how long was I off the Histonet? I can not stand any one who belittles or looks down on some one else because they lack experience or knowledge. Don't we get that enough from the pathologists? We all must remember that at some point, and it might have been many moons ago, that we, did not have the experience or the knowledge that we have today. Can you imagine how much knowledge is spread because of the Histonet? Can you imagine what could have been if the Histonet was in existence 30-40 years ago? Joe ----- Original Message ----- From: "Webb, Dorothy L" To: Sent: Thursday, January 10, 2008 11:19 AM Subject: [Histonet] Agree to disagree I agree with Glen inasmuch as I do not do a lot of "post" responses due to being attacked! Constructive criticism is great and offering gentle advice is even better, but not "putting one down" because they do not know as much as someone else may or have a different viewpoint!! I still LOVE the histonet and appreciate all of the great wisdom instilled in me by those of you who keep responding to my inquiries and others!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From rjbuesa <@t> yahoo.com Fri Jan 11 08:52:05 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 11 08:52:29 2008 Subject: [Histonet] lipoma fixation(for paraffin embedding) In-Reply-To: <1910033357.221321200061304449.JavaMail.osg@osgjas04.cns.ufl.edu> Message-ID: <623113.44793.qm@web61221.mail.yahoo.com> Probably what they want to do is to fix it in FAA which is a mixture of formalin + acetic acid + ethanol. It works fine and fixes rather quickly. Ren? J. "FU,DONGTAO" wrote: --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From mcauliff <@t> umdnj.edu Fri Jan 11 09:00:17 2008 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Jan 11 09:00:48 2008 Subject: [Histonet] Paneth cell dyes or markers In-Reply-To: <7E0A77BFEB9A1E47A63F978E7116821F07986F70@1upmc-msx11.acct.upmchs.net> References: <6BB8BC4519AAB844B174FC739A679BBCCEFFAB@IRMEXCH01.irm.inhs.org> <7E0A77BFEB9A1E47A63F978E7116821F07986F70@1upmc-msx11.acct.upmchs.net> Message-ID: <47878481.7040708@umdnj.edu> PAS works well after buffered formalin or Bouin fixation. If you want to get cute, follow the PAS with Alcian blue, pH 2.5. The rim of most of the granules will be blue. These stains are not specific for Paneth cells, but they will demonstrate them very nicely. An immuno stain for lysozyme should also work. Geoff Yu, Jian wrote: > Does anyone know a good Paneth cell specific dye or IHC marker that > works on paraffin sections of mouse small intestine? > > Thanks a lot! > > Jian Yu, Ph.D. > University of Pittsburgh Cancer Institute > Hillman Cancer Center Research Pavilion > Office Suite 2.26h, Laboratory 2.43 > 5117 Centre Avenue, Pittsburgh, PA 15213 > > Phone: 412-623-7786, (Lab) 412-623-3255 > Fax: 412-623-7778 > Email: yuj2@upmc.edu > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From njoydobro <@t> aol.com Fri Jan 11 09:01:40 2008 From: njoydobro <@t> aol.com (njoydobro@aol.com) Date: Fri Jan 11 09:03:01 2008 Subject: [Histonet] Biocare p16 In-Reply-To: References: Message-ID: <8CA227A01A39F56-D18-206C@WEBMAIL-MB03.sysops.aol.com> what about ProExC?? We use it as a replacement for p16. Gene -----Original Message----- From: Dana Settembre To: Histonet (E-mail) ; Laura Jones Sent: Tue, 8 Jan 2008 8:59 am Subject: Re: [Histonet] Biocare p16 We are in the same boat. I guess we all will be. We didn't want to use the entire detection kit either. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> "Jones, Laura" 01/07/08 3:38 PM >>> We are just wondering if anyone else has been affected by Biocare's inability to sell p16 and what you all have done to replace it? We were told by Biocare that we'd have to order the antibody (and the entire detection kit, which we don't want) from a company in Germany - MTM Labs. My Pathologist would appreciate your advice. Thanks in advance! Laura _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ More new features than ever. Check out the new AOL Mail ! - http://webmail.aol.com From mcauliff <@t> umdnj.edu Fri Jan 11 09:10:26 2008 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Jan 11 09:10:48 2008 Subject: [Histonet] lipoma fixation(for paraffin embedding) In-Reply-To: <1910033357.221321200061304449.JavaMail.osg@osgjas04.cns.ufl.edu> References: <1910033357.221321200061304449.JavaMail.osg@osgjas04.cns.ufl.edu> Message-ID: <478786E2.4070201@umdnj.edu> Unbuffered formalin will be acidic but I suggest 10% formalin buffered with 2% calcuim acetate. The calcium improves preservation of lipids. Geoff FU,DONGTAO wrote: > Hi, all > > We will harvest lipomas and was asked to fix them in acid formalin. > Does anyone have experiences on it? How to make acid formalin? or > where to buy it? How long should I fix them? If I use 10%NBF instead > of acid formalin, how long should I fix them? > > Many thanks, > > Ann Dongtao Fu MD, Ph.D > Lab Manager > Dept. of Pathology > Lab phone: 352-273-7752 > Lab FAX: 352-273-7755 > Lab address: D11-50 > PO Box: 100275 > 1600 SW Archer Road > University of Flodrida > Gainesville, FL 32610 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From karenadams <@t> comcast.net Fri Jan 11 09:30:55 2008 From: karenadams <@t> comcast.net (karenadams@comcast.net) Date: Fri Jan 11 09:31:21 2008 Subject: [Histonet] liver needle bx question Message-ID: <011120081530.29266.47878BAF000A970D0000725222070215739C030E0B0E020A9D0E05@comcast.net> ....any input as to what would be optimal fixation time for liver needle bx before processing...and would anyone care to add on which levels they perform routine and special/immuno staining... Thanks, as always... -- Karen Adams Supervisor Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 From rjbuesa <@t> yahoo.com Fri Jan 11 09:33:27 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 11 09:33:53 2008 Subject: [Histonet] liver needle bx question In-Reply-To: <011120081530.29266.47878BAF000A970D0000725222070215739C030E0B0E020A9D0E05@comcast.net> Message-ID: <306114.94001.qm@web61213.mail.yahoo.com> ! hour per every 1 mm diameter. The selection of which stains/level is a choice of your pathologist to make. Ren? J. karenadams@comcast.net wrote: --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From Terry.Marshall <@t> rothgen.nhs.uk Fri Jan 11 09:42:22 2008 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Jan 11 09:42:44 2008 Subject: [Histonet] liver needle bx question Message-ID: <5C0BED61F529364E86309CADEA63FEF2F3AF37@TRFT-EX01.xRothGen.nhs.uk> Rene, You have caught Kemlo disease. Terry Defn: Kemlo disease: A chronic intractable compulsion to strip a reply of the original message, leaving the reader to wonder what the hell this is all about. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 11 January 2008 15:33 To: karenadams@comcast.net; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] liver needle bx question ! hour per every 1 mm diameter. The selection of which stains/level is a choice of your pathologist to make. Ren? J. karenadams@comcast.net wrote: --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ###################################################################### This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. ###################################################################### From rjbuesa <@t> yahoo.com Fri Jan 11 09:59:24 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 11 09:59:45 2008 Subject: [Histonet] liver needle bx question In-Reply-To: <5C0BED61F529364E86309CADEA63FEF2F3AF37@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <271057.91838.qm@web61221.mail.yahoo.com> No because I was vaccinated this last summer! Ren? J. "Marshall Terry Dr, Consultant Histopathologist" --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From MadaryJ <@t> MedImmune.com Fri Jan 11 11:25:48 2008 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Fri Jan 11 11:26:29 2008 Subject: [Histonet] Dendritic Cells/Mast Cells/NK Cells on FFPE mouse Message-ID: Hey there, I notice you were discussing some NK cell staining in mice. Do you have a protocol you would be willing to share or at least an antibody you recommend? I did lots of ihc on mice and stopped for a few years, now I am back into it a bit just doing some odd cd3, 45, f4 80 all working fine. Boss says she would like to look at PCNA and NK cells in these FFPE mouse spleens. Have not asked on the net yet, figured I would ask you first. Many thanks. Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Laboratory One Medimmune Way Gaithersburg, MD 20878 ph 301.398.4745/6360 fx 301.398.9745 To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From MadaryJ <@t> MedImmune.com Fri Jan 11 11:31:40 2008 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Fri Jan 11 11:32:08 2008 Subject: [Histonet] High Complexity vs training(a joke) Message-ID: Does a tech need Super High Intesnity Training to do High Complexity testing? I was just thinking about the acronym is all. Okay, it was a bad joke folks, but it is Friday. Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Laboratory One Medimmune Way Gaithersburg, MD 20878 ph 301.398.4745/6360 fx 301.398.9745 To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From tahseen <@t> brain.net.pk Fri Jan 11 11:33:08 2008 From: tahseen <@t> brain.net.pk (Tahseen) Date: Fri Jan 11 11:38:28 2008 Subject: [Histonet] Purchase p.Blocks or H&E slides Message-ID: <006401c85478$0878c3c0$230b80cb@PDualCore> Does anyone know where I can purchase p.Blocks or H&E slides (normal histology) for medcal college students. Muhammad Tahseen From Jackie.O'Connor <@t> abbott.com Fri Jan 11 11:37:42 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Jan 11 11:38:42 2008 Subject: [Histonet] Stainless steel strainer In-Reply-To: <488417.82293.qm@web61220.mail.yahoo.com> Message-ID: Happy Friday. I'm looking for a vendor for large, about 7 inch, stainless steel strainers, sieve, colander, whatever you want to call it. We use them for draining formalin fixed tissue, and the local supermarket ones just keep corroding. Any ideas? Jackie O' From HornHV <@t> archildrens.org Fri Jan 11 11:51:02 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Jan 11 11:52:12 2008 Subject: [Histonet] Stainless steel strainer In-Reply-To: References: <488417.82293.qm@web61220.mail.yahoo.com> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82A96@EMAIL.archildrens.org> I bought a plastic one at Target... Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Friday, January 11, 2008 11:38 AM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Stainless steel strainer Happy Friday. I'm looking for a vendor for large, about 7 inch, stainless steel strainers, sieve, colander, whatever you want to call it. We use them for draining formalin fixed tissue, and the local supermarket ones just keep corroding. Any ideas? Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rgarhart <@t> system1.net Fri Jan 11 11:55:50 2008 From: rgarhart <@t> system1.net (Robert Garhart) Date: Fri Jan 11 11:55:40 2008 Subject: [Histonet] Job Opening in GA/FL area In-Reply-To: <000201c77d29$5ef145f0$800aa8c0@domain.local> References: <000201c77d29$5ef145f0$800aa8c0@domain.local> Message-ID: HT or HTL opportunity in GA not far from FL This is a great chance to be part of a large and growing group not too far from the waters of the Atlantic. My client is looking for a someone who preferably has at least 4 years of experience and can function independently. This is a FT position. Experience with Derm, IHC, and Special Stains is a plus. Good benefits package and very collegial work environment. Call to further discuss this opportunity today. Robert Garhart Executive Recruiter System 1 Search 678-342-9029 Office rgarhart@system1.net Website: www.system1.net Please Join my Network on Linked In http://www.linkedin.com/in/robertgarhart From MVaughan4 <@t> ucok.edu Fri Jan 11 12:00:00 2008 From: MVaughan4 <@t> ucok.edu (MVaughan4@ucok.edu) Date: Fri Jan 11 12:00:52 2008 Subject: [Histonet] Thanks for cryostat question In-Reply-To: Message-ID: Histonetters, Thanks for your help with the cryostat question. I received a lot of advice from users and distributors. Mel Melville B. Vaughan, Ph. D. Assistant Professor of Biology Campus Coordinator, NSF Sure-Step program University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm ----------------------------------------- **CONFIDENTIALITY** -This email (including any attachments) may contain confidential, proprietary and privileged information. Any unauthorized disclosure or use of this information is prohibited. From Jackie.O'Connor <@t> abbott.com Fri Jan 11 12:00:04 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Jan 11 12:01:05 2008 Subject: [Histonet] Stainless steel strainer In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82A96@EMAIL.archildrens.org> Message-ID: Plastic ones just don't cut it. The holes are too big. We're straining jars of complete rat necropsies, and some of the smaller organs would go through the holes. The mesh strainers we're using are perfect - but they corrode so fast. "Horn, Hazel V" 01/11/2008 11:51 AM To "Jackie M O'Connor" , , cc Subject RE: [Histonet] Stainless steel strainer I bought a plastic one at Target... Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Friday, January 11, 2008 11:38 AM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Stainless steel strainer Happy Friday. I'm looking for a vendor for large, about 7 inch, stainless steel strainers, sieve, colander, whatever you want to call it. We use them for draining formalin fixed tissue, and the local supermarket ones just keep corroding. Any ideas? Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Weber2 <@t> va.gov Fri Jan 11 12:11:09 2008 From: Susan.Weber2 <@t> va.gov (Weber, Susan (VHACLE)) Date: Fri Jan 11 12:12:19 2008 Subject: [Histonet] Stainless steel strainer In-Reply-To: References: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82A96@EMAIL.archildrens.org> Message-ID: <16C83872A53F4346AA9C3A18E3A3AAB902276CB5@VHAV10MSGA1.v10.med.va.gov> I found mine at the local Restaurant supply store. Susan M. Weber, HT(ASCP) Louis Stokes Cleveland VA Medical Center Supervisor, Histology Laboratory (216) 791-3800 ext 6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Friday, January 11, 2008 1:00 PM To: Horn, Hazel V Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] Stainless steel strainer Plastic ones just don't cut it. The holes are too big. We're straining jars of complete rat necropsies, and some of the smaller organs would go through the holes. The mesh strainers we're using are perfect - but they corrode so fast. "Horn, Hazel V" 01/11/2008 11:51 AM To "Jackie M O'Connor" , , cc Subject RE: [Histonet] Stainless steel strainer I bought a plastic one at Target... Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Friday, January 11, 2008 11:38 AM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Stainless steel strainer Happy Friday. I'm looking for a vendor for large, about 7 inch, stainless steel strainers, sieve, colander, whatever you want to call it. We use them for draining formalin fixed tissue, and the local supermarket ones just keep corroding. Any ideas? Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Fri Jan 11 12:59:24 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Fri Jan 11 13:01:43 2008 Subject: OT: [Histonet] Agree to disagree References: <0E394B648E5284478A6CCB78E5AFDA27056353B9@hpes1.HealthPartners.int><008201c85401$62ff2d10$0302a8c0@yourxhtr8hvc4p> <004901c85461$05550d00$3d02a8c0@plab.local> Message-ID: <08A0A863637F1349BBFD83A96B27A50A1200C5@uwhis-xchng3.uwhis.hosp.wisc.edu> Whoa! Cheri said 'Joe' and 'grace' in the same paragraph! :) Happy Monday everyone Claire Very well said Joe, We get kicked around enough by pathologists etc. Our peers should be supportave! I for one am happy you still grace us with your wisdom and wit!.....Cheri From vcj6 <@t> cdc.gov Fri Jan 11 13:35:50 2008 From: vcj6 <@t> cdc.gov (Johnson, Vic (CDC/NIOSH/HELD)) Date: Fri Jan 11 13:39:47 2008 Subject: [Histonet] Ccr3 immunohistochemistry in mouse tissue Message-ID: <20D8129D96E39249BF918BFDF207823103D5631B@m-niosh-3.niosh.cdc.gov> Dear list members, I am new to the list as this is my first post. I have a question about chemokine receptor 3 immunohistochemistry. Does anyone have experience finding this antigen in mouse tissue? Is there a recommended positive control? We have a mouse model of allergic rhinitis and know that there are plenty of eosinophils and Th2 lymphocytes which should stain for this receptor. Any help will be greatly appreciated. Thanks, Vic Vic Johnson, Ph.D. Chronic Inflammatory & Immune Disease Team Toxicology and Molecular Biology Branch Health Effects Laboratory Division NIOSH/CDC 1095 Willowdale Road, M/S 3014 Morgantown, WV 26505-2888 Tel. +1 304-285-6249 Fax +1 304-285-5708 Email: VJohnson3@cdc.gov From contact <@t> excaliburpathology.com Fri Jan 11 13:40:23 2008 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Fri Jan 11 13:40:46 2008 Subject: [Histonet] Looking for the Quackenbushes Message-ID: <9808.87094.qm@web50109.mail.re2.yahoo.com> Hello, I am looking for Bob and Debbie Quackenbush. Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-759-3953 contact@excaliburpathology.com www.excaliburpathology.com From contact <@t> excaliburpathology.com Fri Jan 11 13:44:49 2008 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Fri Jan 11 13:45:12 2008 Subject: [Histonet] Stainless Steel Strainer Message-ID: <429342.23319.qm@web50103.mail.re2.yahoo.com> Try using a plastic strainer lined with cheesecloth. Works great catching seeds when making jelly. :) Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-759-3953 contact@excaliburpathology.com www.excaliburpathology.com From rjbuesa <@t> yahoo.com Fri Jan 11 14:18:16 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 11 14:18:38 2008 Subject: [Histonet] Stainless steel strainer In-Reply-To: Message-ID: <355377.88070.qm@web61211.mail.yahoo.com> Buy a solid plastic one and bore holes with a Dremel. Ren? J. Jackie M O'Connor wrote: Plastic ones just don't cut it. The holes are too big. We're straining jars of complete rat necropsies, and some of the smaller organs would go through the holes. The mesh strainers we're using are perfect - but they corrode so fast. "Horn, Hazel V" 01/11/2008 11:51 AM To "Jackie M O'Connor" , , cc Subject RE: [Histonet] Stainless steel strainer I bought a plastic one at Target... Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Friday, January 11, 2008 11:38 AM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Stainless steel strainer Happy Friday. I'm looking for a vendor for large, about 7 inch, stainless steel strainers, sieve, colander, whatever you want to call it. We use them for draining formalin fixed tissue, and the local supermarket ones just keep corroding. Any ideas? Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From llewllew <@t> shaw.ca Fri Jan 11 14:46:51 2008 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Fri Jan 11 14:48:55 2008 Subject: [Histonet] Stainless steel strainer References: <355377.88070.qm@web61211.mail.yahoo.com> Message-ID: <001401c85493$17b93ac0$05024246@yourlk4rlmsu> Try using a permanent (reusable) coffee filter. They come in fine mesh plastic, stainless steel or gold plated (for coffee purists). They are tough and designed to filter out fine grind coffee. Plus, they come in a variety of shapes and sizes. Bryan Llewellyn > Jackie M O'Connor wrote: > Plastic ones just don't cut it. The holes are too big. We're straining > jars of complete rat necropsies, and some of the smaller organs would go > through the holes. The mesh strainers we're using are perfect - but they > corrode so fast. > > > > > "Horn, Hazel V" > 01/11/2008 11:51 AM > > To > "Jackie M O'Connor" , > , > > cc > > Subject > RE: [Histonet] Stainless steel strainer > > > > > > > I bought a plastic one at Target... > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Histology > Arkansas Children's Hospital > 800 Marshall Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax 501.364.3912 > > visit us on the web at: www.archildrens.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M > O'Connor > Sent: Friday, January 11, 2008 11:38 AM > To: histonet@lists.utsouthwestern.edu; > histonet-bounces@lists.utsouthwestern.edu > Subject: [Histonet] Stainless steel strainer > > Happy Friday. I'm looking for a vendor for large, about 7 inch, > stainless > steel strainers, sieve, colander, whatever you want to call it. We > use > them for draining formalin fixed tissue, and the local supermarket ones > just keep corroding. > Any ideas? > > Jackie O' > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it > now. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Fri Jan 11 16:17:07 2008 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Jan 11 16:17:31 2008 Subject: [Histonet] Harford Community Graduates? Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F48B2@sjhaexc02.sjha.org> Hello Everyone.. I am interested in feedback regarding this Histology program. Anyone have any experience or comments? Thanks! Happy weekend! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax From sprice2003 <@t> gmail.com Fri Jan 11 16:37:53 2008 From: sprice2003 <@t> gmail.com (Sally Price) Date: Fri Jan 11 16:38:17 2008 Subject: [Histonet] Deterioration rate of fluors used in IF staining Message-ID: Netters: I realize that the fluorescnt dyes like Alexa/Dylight deteriorate over time, but I was wondering: Just how fast? For years we've been told to protect reagents from direct light during the staining procedure and to keep stained slides in the dark when not under the scope, but how critical is this -- and does keeping the reagents/slides refrigerated slow down the process, or am I just wasting the effort? Maybe some vendors can chime in here too. Cheers! -- Sally Price From Charlene.Henry <@t> STJUDE.ORG Fri Jan 11 17:03:16 2008 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Fri Jan 11 17:03:37 2008 Subject: [Histonet] Viral and Fungal Antibodies and Probes Message-ID: <5CB39BCA5724F349BCB748675C6CA1A2145436F1@SJMEMXMB02.stjude.sjcrh.local> Hello Fellow Histonetters I need help locating a vendor/vendors that might carry the following viral and fungal primary antibodies and probes. Any help will be appreciated. Measles Human Herpes Virus 6A,B RSV Vaccinia Monkeypox Other Organisms - Blastomyces dermatitidis - Coccidioides immitis - Histoplasma capsulatum - Crytococcus neoformans - Penicillium marneffei - Sporothrix schenckii - Paracoccidiodes brasiliensis - Candida species - Pneumocystis - Toxoplasma gondii - Leishmania species - Trypanosoma cruzi - Balantidium coli - Basidiobolus ranarum - Entamoeba species (please note if specific species are available, esp E histolytica, E coli, E dispar) - Endolimax nana - Iodamoeba buetschlii Charlene Henry HT (ASCP), QIHC Anatomic Pathology Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 From shive003 <@t> umn.edu Fri Jan 11 17:21:58 2008 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Jan 11 17:22:21 2008 Subject: [Histonet] Viral and Fungal Antibodies and Probes References: <5CB39BCA5724F349BCB748675C6CA1A2145436F1@SJMEMXMB02.stjude.sjcrh.local> Message-ID: <015801c854a8$c3725cb0$b0065486@auxs.umn.edu> Toxoplasma gondii - LabVision; cat. # RB-282 Jan Shivers Senior Scientist Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu ----- Original Message ----- From: "Henry, Charlene" To: Sent: Friday, January 11, 2008 5:03 PM Subject: [Histonet] Viral and Fungal Antibodies and Probes Hello Fellow Histonetters I need help locating a vendor/vendors that might carry the following viral and fungal primary antibodies and probes. Any help will be appreciated. Measles Human Herpes Virus 6A,B RSV Vaccinia Monkeypox Other Organisms - Blastomyces dermatitidis - Coccidioides immitis - Histoplasma capsulatum - Crytococcus neoformans - Penicillium marneffei - Sporothrix schenckii - Paracoccidiodes brasiliensis - Candida species - Pneumocystis - Toxoplasma gondii - Leishmania species - Trypanosoma cruzi - Balantidium coli - Basidiobolus ranarum - Entamoeba species (please note if specific species are available, esp E histolytica, E coli, E dispar) - Endolimax nana - Iodamoeba buetschlii Charlene Henry HT (ASCP), QIHC Anatomic Pathology Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Fri Jan 11 18:18:02 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Jan 11 18:18:16 2008 Subject: [Histonet] Agree to disagree References: <0E394B648E5284478A6CCB78E5AFDA27056353B9@hpes1.HealthPartners.int> <008201c85401$62ff2d10$0302a8c0@yourxhtr8hvc4p> <004901c85461$05550d00$3d02a8c0@plab.local> <08A0A863637F1349BBFD83A96B27A50A1200C5@uwhis-xchng3.uwhis.hosp.wisc.edu> Message-ID: <008b01c854b0$98e39c90$0302a8c0@yourxhtr8hvc4p> hey!!!! What's up with that? There are trees in New England that have Joe and Grace etched in them. Oops, never mind, wrong grace. ----- Original Message ----- From: "Ingles Claire" To: Sent: Friday, January 11, 2008 12:59 PM Subject: OT: [Histonet] Agree to disagree Whoa! Cheri said 'Joe' and 'grace' in the same paragraph! :) Happy Monday everyone Claire Very well said Joe, We get kicked around enough by pathologists etc. Our peers should be supportave! I for one am happy you still grace us with your wisdom and wit!.....Cheri _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marjoh3 <@t> telus.net Sat Jan 12 08:10:42 2008 From: marjoh3 <@t> telus.net (Marilyn Johnson) Date: Sat Jan 12 08:10:53 2008 Subject: [Histonet] Demonstration of Blue Tongue by Immunohistochemistry Message-ID: <003301c85524$eb2ede00$6501a8c0@VALUED20606295> Hi Histonetters, Our Lab. is interested in demonstrating Blue Tongue in sheep. This project is scheduled to begin in March. If there are any veterinary labs currently performing IHC, would you please share your protocols for staining and the reagents/supplier with me. Any replies would be greatly appreciated. Thank you in advance. Marilyn Johnson Alberta Agriculture Edmonton, Alberta, Canada, From rjbuesa <@t> yahoo.com Sat Jan 12 09:50:27 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Jan 12 09:50:51 2008 Subject: [Histonet] Deterioration rate of fluors used in IF staining In-Reply-To: Message-ID: <848944.54594.qm@web61216.mail.yahoo.com> Sally: The IF reagents I used (FITC conjugated Ig) I used to aliquot them and keep them at -80?C until needed. Once the slide was stained I always tried to read as soon as possible. Otherwise I kept the slides on a refrigerator for, the most 3 days, and after that the fluorescence faded. There are coverslipping solutions that reduce the fading rate also. The best way of keeping fluorescent reagents is in a refrigerator (where they are also protected from light). Ren? J. Sally Price wrote: --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From conniegrubaugh <@t> hotmail.com Sat Jan 12 10:00:58 2008 From: conniegrubaugh <@t> hotmail.com (connie grubaugh) Date: Sat Jan 12 10:01:23 2008 Subject: [Histonet] auroa dx Message-ID: It there are any techs out there who's company was bought by Aurora DX would you please contact me. I have a few questions. thanks Connie Grubaugh. _________________________________________________________________ Get the power of Windows + Web with the new Windows Live. http://www.windowslive.com?ocid=TXT_TAGHM_Wave2_powerofwindows_012008 From gayle.callis <@t> bresnan.net Sat Jan 12 16:05:22 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Sat Jan 12 16:05:44 2008 Subject: [Histonet] Viral and Fungal Antibodies and Probes References: <5CB39BCA5724F349BCB748675C6CA1A2145436F1@SJMEMXMB02.stjude.sjcrh.local> Message-ID: <002801c85567$39f21ce0$6501a8c0@DHXTS541> Richard Cartun has always recommended VIROSTAT out of Maine. They have a huge array of antibodies to infectious organisms. Google their name for their website. Also, you can go to another website, www.exactantigen to find antibodies. Gayle M. Callis HT/HTL/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Henry, Charlene" To: Sent: Friday, January 11, 2008 4:03 PM Subject: [Histonet] Viral and Fungal Antibodies and Probes Hello Fellow Histonetters I need help locating a vendor/vendors that might carry the following viral and fungal primary antibodies and probes. Any help will be appreciated. Measles Human Herpes Virus 6A,B RSV Vaccinia Monkeypox Other Organisms - Blastomyces dermatitidis - Coccidioides immitis - Histoplasma capsulatum - Crytococcus neoformans - Penicillium marneffei - Sporothrix schenckii - Paracoccidiodes brasiliensis - Candida species - Pneumocystis - Toxoplasma gondii - Leishmania species - Trypanosoma cruzi - Balantidium coli - Basidiobolus ranarum - Entamoeba species (please note if specific species are available, esp E histolytica, E coli, E dispar) - Endolimax nana - Iodamoeba buetschlii Charlene Henry HT (ASCP), QIHC Anatomic Pathology Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Sat Jan 12 16:21:44 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Sat Jan 12 16:22:08 2008 Subject: [Histonet] Deterioration rate of fluors used in IF staining References: Message-ID: <002e01c85569$8366d260$6501a8c0@DHXTS541> We now coverslip with Molecular Probes Prolong Gold antifade reagent, with or without DAPI. We have found some antifade mounting medias cause fading if left overnight, but if the slides are examined right after staining, fading has not occured. So be careful about what antifade mounting medias you use. Also, some fluorophores do not withstand mounting medias and will fade right away. I believe Cy2 is one, and also Alexa 630 - Jackson and Molecular Probes provide this information. We have found RRX (rhodamine red extra) from Jackson Immunorearch, is more reisistant to staining than TRITC, both are derivatives from fluorescein but have different chemical structures. We don't bother to refrigerate but do keep the sections in the dark and try to view them the next day after staining,coverslipping. Selection of fluorophore is important, and Alexa dyes are more resistant to fading and also much brighter than fluorescein related fluorophores (FITC, Rhodamine, Texas Red). It is important to learn about the fluorophores and their chemstry. The experts on this are Invitrogen/Molecular Probes. Alexa 488 dye will not fade as fast as FITC, but it will fade eventually - one can always test this by staining several sections and pulling one a day until the fluorophore has faded. All staining with fluorophore steps are performed under cover of a black towel to protect from any light, and when making up the reagents, keeping the diluted fluorophore conjugates in a drawer before application is important. It isn't the cold that prevents fading, it is lessening the exposure to light. Most of the time, refrigerators are closed with the lights off, and slides are kept in the dark in a cardboard flat/folder, essentially protected from the light until examination. We have put slides in a drawer if we can't examine them the same or next day. We do store fluorophore conjugated reagents according to manufacturer instructions, in a lidded, dark box, in a refrigerator or freezer in a light protected box. Gayle M. Callis HT/HTL/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Sally Price" To: Sent: Friday, January 11, 2008 3:37 PM Subject: [Histonet] Deterioration rate of fluors used in IF staining > Netters: > I realize that the fluorescnt dyes like Alexa/Dylight deteriorate over > time, > but I was wondering: Just how fast? For years we've been told to protect > reagents from direct light during the staining procedure and to keep > stained > slides in the dark when not under the scope, but how critical is this -- > and > does keeping the reagents/slides refrigerated slow down the process, or am > I > just wasting the effort? Maybe some vendors can chime in here too. > Cheers! > -- > Sally Price > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jdmd77 <@t> hotmail.com Sun Jan 13 11:46:20 2008 From: jdmd77 <@t> hotmail.com (Julia Dahl) Date: Sun Jan 13 11:46:45 2008 Subject: [Histonet] auroa dx In-Reply-To: References: Message-ID: Connie - You may be interested to talk to anyone who was bought by AmeriPath, as well - since these are the same cast of characters (at least at the top). J > From: conniegrubaugh@hotmail.com > To: histonet@lists.utsouthwestern.edu > Date: Sat, 12 Jan 2008 08:00:58 -0800 > Subject: [Histonet] auroa dx > > It there are any techs out there who's company was bought by Aurora DX would you please contact me. I have a few questions. > > > thanks > > Connie Grubaugh. > > _________________________________________________________________ > Get the power of Windows + Web with the new Windows Live. > http://www.windowslive.com?ocid=TXT_TAGHM_Wave2_powerofwindows_012008_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Make distant family not so distant with Windows Vista? + Windows Live?. http://www.microsoft.com/windows/digitallife/keepintouch.mspx?ocid=TXT_TAGLM_CPC_VideoChat_distantfamily_012008 From babears <@t> triad.rr.com Sun Jan 13 14:29:29 2008 From: babears <@t> triad.rr.com (Pete and Sharon Jack) Date: Sun Jan 13 14:29:51 2008 Subject: [Histonet] Aurora Message-ID: <001101c85622$ffd90a70$90e84c47@pete38d56c4d11> Our laboratory was purchased by Auror on October 2, 2007. It's too soon to tell how things will be. We did lose our profit sharing which was quite lucrative. Sharon S. Jack, HT(ASCP) From esther.peters <@t> verizon.net Sun Jan 13 16:22:13 2008 From: esther.peters <@t> verizon.net (Esther Peters) Date: Sun Jan 13 16:22:33 2008 Subject: [Histonet] Who can perform Histology Duties In-Reply-To: <5C0BED61F529364E86309CADEA63FEF2F3AF35@TRFT-EX01.xRothGen.nhs.uk> References: <5C0BED61F529364E86309CADEA63FEF2F3AF35@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <478A8F15.5050904@verizon.net> Terry, Enjoyed your comments. Training, continuing education, and especially knowing when you should refer to someone else are key in any profession! Regarding "norma,l" the pathologists are not using "some sort of mealy mouthed evasion" but they know that there is a "range" of normalcy when identifying the condition of cells and tissues, because of individual variation, age, reproductive status, nutritional status, etc. Just think of the wide range in values for what are considered "nondiseased" levels of various blood parameters. I once corresponded with a professor of histology, who told me he only knew "normal" and didn't know anything about pathology. But if you don't understand structural changes indicating diseases, are you sure you can recognize "normal"? Esther Peters, Ph.D. George Mason University Marshall Terry Dr, Consultant Histopathologist wrote: > "Proper training can allow Histotech's to report normal, ....." > > There was a bizarre discussion on the histopathology discussion group > recently, around reporting tissue as "normal". > An amazing number of pathologists never used the word, but preferred > some sort of mealy mouthed evasion. > Within normal limits, no definite lesion seen ... That sort of thing. > Indeed one very competent pathologist claimed he could not recognise > normal! > > Terry > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo > Rogerson > Sent: 10 January 2008 16:08 > To: Terri Braud; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Who can perform Histology Duties > > Please save your flames. The scenerio of a Non-Histotech embedding, > cutting, and staining my surgical tissue does not thrill me, but that > was not her question. Maybe the reality of the answer is why many > Histology Labs have always been considered the evil red-headed > stepchildren of the Lab. > Terri > > I think you are correct in that as long as someone is properly trained, > the training is revisited regularly and there is adequate supervision > and the professional groups are in agreement Non-BMS (Non-Histotech) > Staff can do many jobs in Histology and maybe Non Medical Staff (BMS) > can carry out some interpretation. I would have no issues with a > Non-Histotech (Non-BMS) doing as you say with proper supervision by a > HPC registered supervisor. > > But where does it end? Do you need a medical degree to identify vas, > appendix or gall bladder? You need the training to know when you have > reached the limits of your knowledge and when to refer to someone better > able to report a disease and I concede that is probably a Medic. Proper > training can allow Histotech's to report normal, maybe non-normal > requires medical insight, what do you think? > > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > Don't be afraid to take a big step when one is indicated. You can't > cross a chasm in two small jumps. --Buckminster Fuller > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ###################################################################### > This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. > The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. > If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. > ###################################################################### > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From minhan.science <@t> gmail.com Sun Jan 13 23:58:13 2008 From: minhan.science <@t> gmail.com (Min-Han Tan) Date: Sun Jan 13 23:58:37 2008 Subject: [Histonet] RBC removal Message-ID: <7902152a0801132158x2be7da3dxb0649bd086c4f25d@mail.gmail.com> Dear fellow list members, I have been trying to remove RBCs from bloody pleural effusions / abdominal ascites using Histopaque, with low success, for the purpose of cytology. Histopaque works great on blood. I wonder if any one has successfully worked on bloody effusions / ascites previously; they do not seem to centrifuge in as optimal a fashion as blood, and I wonder if there are proteins / free hemoglobin that may be altering the density somewhat. Has anyone used ACK buffer or any other such methods to remove / lyse RBCs? Thanks! Regards, Min-Han Tan From olek.michalski <@t> nencki.gov.pl Mon Jan 14 00:39:16 2008 From: olek.michalski <@t> nencki.gov.pl (Olek Michalski) Date: Mon Jan 14 00:33:55 2008 Subject: [Histonet] BrdU/DCX problem In-Reply-To: <001a01c853b4$34648240$1d6413ac@C26124> References: <011101c843e3$340418a0$1d6413ac@C26124> <001a01c853b4$34648240$1d6413ac@C26124> Message-ID: Dear Jon, 2008-01-10 19:11:20 Jonathan Frank wrote: [...] > Thanks for the response. Do you do 1st primary, 1st secondary, then brdU > primary, and BrdU secondary? [...] It is exactly the case. If you like I can ask my coleague, who actually worked out the protocol and uses it routinely for a detailed list of opertions with some comments. At first glance I see some differences in BrdU labelling procedure. > Also, which BrdU primary antibody do you use? For BrdU we use antibody from Roche. I don't remember the catalogue no. and the characteristics I can found at the moment is a bit old but I suppose they offer only one kind. Best regards Olek -- Laboratory of Neurobiology of Development and Evolution Nencki Institute of Experimental Biology ul. Pasteura 3, 02-093 Warszawa, Poland Tel. +48 22 5892268, Fax +48 22 8225342 From Terry.Marshall <@t> rothgen.nhs.uk Mon Jan 14 06:26:00 2008 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Mon Jan 14 06:26:22 2008 Subject: [Histonet] Who can perform Histology Duties Message-ID: <5C0BED61F529364E86309CADEA63FEF2F3AF41@TRFT-EX01.xRothGen.nhs.uk> Odd that there should be only one comment on my post, because I thought it (the subject rather than my post) was an interesting one. It's impossible to judge just what gets people interested. Anyway, back at the ranch, you are right that there is a range of appearances, but that range is only of significance in breast and prostate, of the things we see commonly, and it would certainly be true that "normal" has very fuzzy edges in these organs. However, for the rest, there is no significant range. Large bowel biopsies actually gives rise to problems too, as they are almost always abnormal, but only mildly so, and unfortunately, not showing any features by which one can make a diagnosis. I usually report these as no significant abnormality, or similar, but sometimes I just call them normal, as it really makes no practical difference. As these patients have bowel problems, usually diarrhoea, and many have had some sort of bowel prep., it is no surprise that the appearance should not be "normal" in the sense of ideal. It's all a matter of how you look at things, and in US in particular, how well you like to cover your ass. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Esther Peters Sent: 13 January 2008 22:22 To: Marshall Terry Dr, Consultant Histopathologist Cc: histonet@lists.utsouthwestern.edu; Terri Braud; Kemlo Rogerson Subject: Re: [Histonet] Who can perform Histology Duties Terry, Enjoyed your comments. Training, continuing education, and especially knowing when you should refer to someone else are key in any profession! Regarding "norma,l" the pathologists are not using "some sort of mealy mouthed evasion" but they know that there is a "range" of normalcy when identifying the condition of cells and tissues, because of individual variation, age, reproductive status, nutritional status, etc. Just think of the wide range in values for what are considered "nondiseased" levels of various blood parameters. I once corresponded with a professor of histology, who told me he only knew "normal" and didn't know anything about pathology. But if you don't understand structural changes indicating diseases, are you sure you can recognize "normal"? Esther Peters, Ph.D. George Mason University Marshall Terry Dr, Consultant Histopathologist wrote: > "Proper training can allow Histotech's to report normal, ....." > > There was a bizarre discussion on the histopathology discussion group > recently, around reporting tissue as "normal". > An amazing number of pathologists never used the word, but preferred > some sort of mealy mouthed evasion. > Within normal limits, no definite lesion seen ... That sort of thing. > Indeed one very competent pathologist claimed he could not recognise > normal! > > Terry > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo > Rogerson > Sent: 10 January 2008 16:08 > To: Terri Braud; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Who can perform Histology Duties > > Please save your flames. The scenerio of a Non-Histotech embedding, > cutting, and staining my surgical tissue does not thrill me, but that > was not her question. Maybe the reality of the answer is why many > Histology Labs have always been considered the evil red-headed > stepchildren of the Lab. > Terri > > I think you are correct in that as long as someone is properly > trained, the training is revisited regularly and there is adequate > supervision and the professional groups are in agreement Non-BMS > (Non-Histotech) Staff can do many jobs in Histology and maybe Non > Medical Staff (BMS) can carry out some interpretation. I would have no > issues with a Non-Histotech (Non-BMS) doing as you say with proper > supervision by a HPC registered supervisor. > > But where does it end? Do you need a medical degree to identify vas, > appendix or gall bladder? You need the training to know when you have > reached the limits of your knowledge and when to refer to someone > better able to report a disease and I concede that is probably a > Medic. Proper training can allow Histotech's to report normal, maybe > non-normal requires medical insight, what do you think? > > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > Don't be afraid to take a big step when one is indicated. You can't > cross a chasm in two small jumps. --Buckminster Fuller > > This e-mail is confidential and privileged. If you are not the > intended recipient please accept my apologies; please do not disclose, > copy or distribute information in this e-mail or take any action in > reliance on its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ###################################################################### > This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. > The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. > If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. > ###################################################################### > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Mon Jan 14 08:01:10 2008 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Jan 14 08:01:44 2008 Subject: [Histonet] RELIA Histology Opportunity Update 01-14-08 Happy New Year! Message-ID: Hi Histonetters! What?s your New Year?s Resolution? Of course I will try to eat right and exercise. But My Resolution is to help even more histo techs find the right opportunity in the right place at the right time. In 2007 I helped people make the move into a management role, relocate closer to family, shorten their commute, move into research, utilize their advanced degrees, get better salaries, shifts and benefits. In 2008 I resolve to help even more people make the changes they want to make in order to improve their career situations and ultimately their quality of life. Whatever you want to do Wherever you want to be. Let Me Help! Take Advantage of RELIA?s Career Coaching Program. Free of Charge RELIA offers: * Assistance with updating or creating your resume * Tips on interviewing * Encouragement and Assistance during the course of your job search * Responsiveness ? I will respond to your e-mails/phone calls within 24 hours or less. * Troubleshooting ? if your job search is stalled or you can?t get in the company you are interested in. * Personalized Job Search ? customized to your experience, wants and needs. * Complete Confidentiality - Your career and my coaching is a relationship of trust. I have a nationwide network of contacts with the premier employers of histology professionals in clinical, research and biotech settings. I only work with full time permanent positions at companies that offer excellent compensation, benefits programs and relocation assistance. Here are some of my current openings: Histology Managers/Supervisors/Specialists needed in: Dallas, TX - Clinical Los Angeles, CA - Clinical Seattle, WA ? Research/Clinical Waco, TX ? Clinical Immunohistochemistry Histotechnicians/Histotechnologists needed in: Phoenix, AZ - Clinical Los Angeles, CA - Clinical Los Angeles, CA ? oppty to learn MOHS Orlando, FL - Dermatopathology Pittsburgh, PA - Clinical Cincinatti, OH - Clinical Austin, TX - Clinical Waco, TX - Clinical Concord, NH ? Clinical Seattle, WA ? Research/Clinical Helena, MT ? Clinical ?Heads Up? These are areas where some of my clients project a need in the near future. Indianapolis, IN Ft. Lauderdale, FL Boston, MA Ann Arbor, MI Rhode Island If you or any of your friends would like more information on RELIA?S Career Coaching or about any of the positions listed please contact me. I would be more than happy to assist you. You can reach me at 866-607-3542 or relia1@earthlink.net Remember it never hurts to look Hope to hear from you soon . Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.myspace.com/pamatrelia From burch007 <@t> mc.duke.edu Mon Jan 14 14:33:56 2008 From: burch007 <@t> mc.duke.edu (James L Burchette) Date: Mon Jan 14 14:32:16 2008 Subject: [Histonet] 2030 Microtome Part Message-ID: Dear Histonetters: I am looking for an X Y object holder locking lever for an old Reichert Jung or Leica 2030 microtome. The microtome still works great (one owner and used with care), but the locking lever has broken from years of stress. I will be glad to pay for shipping. Thanks, JB Jim Burchette, HT(ASCP) QIHC "A simple histotech from a little country hospital in North Carolina" burch007@mc.duke.edu 919.681.3973 From failm <@t> musc.edu Mon Jan 14 14:45:20 2008 From: failm <@t> musc.edu (Fail, Mildred M.) Date: Mon Jan 14 14:46:14 2008 Subject: [Histonet] 15 MORE Message-ID: 15 more working days until I retire!!! Great job opening here at MUHA in Charleston, SC, From Stephen.Clark1 <@t> hcahealthcare.com Mon Jan 14 15:10:38 2008 From: Stephen.Clark1 <@t> hcahealthcare.com (Clark Stephen - Myrtle Beach) Date: Mon Jan 14 15:16:51 2008 Subject: [Histonet] 15 MORE In-Reply-To: References: Message-ID: <28E40C736ED32341BBB2B094EFD23FC8020F0687@NASEV05.hca.corpad.net> I know that this was just a topic, but my lab manager is inquiring as to the requirements of histotechs grossing specimens. Does anyone know the CLIA requirements and where to find them? Thanks, Steve Clark Histology Supervisor Grand Strand Regional Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fail, Mildred M. Sent: Monday, January 14, 2008 3:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] 15 MORE 15 more working days until I retire!!! Great job opening here at MUHA in Charleston, SC, _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Robert.Fauck <@t> ccdhb.org.nz Mon Jan 14 15:28:57 2008 From: Robert.Fauck <@t> ccdhb.org.nz (Robert Fauck [CCDHB]) Date: Mon Jan 14 15:29:30 2008 Subject: [Histonet] hISTOcHEMICAL STAIN FOR GANGLION cELLS Message-ID: <5587F098B7C4EE489C8BE204E9565548248CF6@wn0nteml05.hiq.net.nz> Hi Everybody, Does somebody can help me with a reliable (fast) stain for Ganglion cells for Hirsch Sprung Disease?! Our ACHE stain for it is playing up at the moment, I think it is due to the age of our Tetra isopropyl pyrophosphoramide ( OMPA ) chemical in the freezer. Is it adequate to use a fresh piece of Appendix for a control? Thanking you in advance for your help. Regards, Robert Fauck Wellington Hospital New Zealand This email or attachment(s) may contain confidential or legally privileged information intended for the sole use of the addressee(s). Any use, redistribution, disclosure, or reproduction of this message, except as intended, is prohibited. If you received this email in error, please notify the sender and remove all copies of the message, including any attachments. Any views or opinions expressed in this email (unless otherwise stated) may not represent those of Capital & Coast District Health Board. http://www.ccdhb.org.nz (1C_S1) No Viruses were detected in this message. HealthIntelligence eMail Filter Service From jstaruk <@t> masshistology.com Mon Jan 14 17:18:32 2008 From: jstaruk <@t> masshistology.com (jstaruk) Date: Mon Jan 14 17:19:06 2008 Subject: [Histonet] hISTOcHEMICAL STAIN FOR GANGLION cELLS In-Reply-To: <5587F098B7C4EE489C8BE204E9565548248CF6@wn0nteml05.hiq.net.nz> Message-ID: Histologic has a nice article on staining ganglion cells with toluidine blue in its May, 2007 issue. I believe you can get this article on-line through the Sakura web site. Jim _____________________ Jim Staruk Mass Histology Service www.masshistology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Fauck [CCDHB] Sent: Monday, January 14, 2008 4:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] hISTOcHEMICAL STAIN FOR GANGLION cELLS Hi Everybody, Does somebody can help me with a reliable (fast) stain for Ganglion cells for Hirsch Sprung Disease?! Our ACHE stain for it is playing up at the moment, I think it is due to the age of our Tetra isopropyl pyrophosphoramide ( OMPA ) chemical in the freezer. Is it adequate to use a fresh piece of Appendix for a control? Thanking you in advance for your help. Regards, Robert Fauck Wellington Hospital New Zealand This email or attachment(s) may contain confidential or legally privileged information intended for the sole use of the addressee(s). Any use, redistribution, disclosure, or reproduction of this message, except as intended, is prohibited. If you received this email in error, please notify the sender and remove all copies of the message, including any attachments. Any views or opinions expressed in this email (unless otherwise stated) may not represent those of Capital & Coast District Health Board. http://www.ccdhb.org.nz (1C_S1) No Viruses were detected in this message. HealthIntelligence eMail Filter Service _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dont8know8me <@t> yahoo.com Mon Jan 14 23:56:28 2008 From: dont8know8me <@t> yahoo.com (richard peralta) Date: Mon Jan 14 23:56:50 2008 Subject: [Histonet] (no subject) Message-ID: <277747.54213.qm@web51403.mail.re2.yahoo.com> Im looking for MR William DeSalvo of Banner Health in ARIZONA ____________________________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ From Sharon.Davis-Devine <@t> carle.com Tue Jan 15 08:29:13 2008 From: Sharon.Davis-Devine <@t> carle.com (Sharon.Davis-Devine) Date: Tue Jan 15 08:29:38 2008 Subject: [Histonet] block filing cabinets Message-ID: <44780C571F28624DBB446DE55C4D733A021E084C@EXCHANGEBE1.carle.com> The company that we have always bought our filing cabinets from for our slide and block filing no longer sells them can some of you point me in the direction of a different company? Also we have limited space as most labs do so how many blocks and slides do you store onsite? Any help or suggestions are greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com From vazquezr <@t> ohsu.edu Tue Jan 15 08:48:32 2008 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Tue Jan 15 08:49:16 2008 Subject: [Histonet] block filing cabinets Message-ID: Sharon, We use Sakura, Tissue-Tek brand and we store the last 3 years on site. Robyn Vazquez OHSU >>> "Sharon.Davis-Devine" 1/15/2008 6:29 AM >>> The company that we have always bought our filing cabinets from for our slide and block filing no longer sells them can some of you point me in the direction of a different company? Also we have limited space as most labs do so how many blocks and slides do you store onsite? Any help or suggestions are greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmmathis1 <@t> bellsouth.net Tue Jan 15 09:18:44 2008 From: cmmathis1 <@t> bellsouth.net (cmmathis1@bellsouth.net) Date: Tue Jan 15 09:19:08 2008 Subject: [Histonet] SCXA antibody Message-ID: <011520081518.8987.478CCED40003D6CB0000231B22243429029B0A02D2089B9A019C04040A0DBFCE9C07089B0E03030C@att.net> Is anyone using SCXA (scleraxis) rabbit polyclonal antibody from Abcam or other vendor? Any information on its dilution range and use would be greatly appreciated. We intend on using it on cultured cells. Cathy M. Mathis Histology Core Technician Wake Forest Institute for Regenerative Medicine Wake Forest University Health Sciences Medical Center Blvd. Winston-Salem, NC 27157 ph: 336-713-7285 fax: 336-713-7290 email: cmmathis@wfubmc.edu From LSebree <@t> uwhealth.org Tue Jan 15 10:37:58 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Tue Jan 15 10:38:21 2008 Subject: [Histonet] WT-1 Message-ID: Good Morning everyone, I was wondering what people are using for WT-1 (Wilm's tumor) antibody. I'm especially interested in this antibody being used with Ventana instrumentation. Thanks, Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From celebrej <@t> HHSC.CA Tue Jan 15 10:43:11 2008 From: celebrej <@t> HHSC.CA (Celebre Julia) Date: Tue Jan 15 10:43:34 2008 Subject: [Histonet] Myth or Fact Message-ID: <0B3BF9D810C7094FA34B17DD145C3D5E056B96F8@ipemail01.hhsc.ca> Is it a myth or fact that Mycobacterium bacilli can carryover from one slide to another if stained in a coplin jar when performing a ZN? If this is true, are we to then deparaffinize slides separately? Julia Celebre MLT Anatomic Pathology Hamilton General Hospital 905-527-0271 ext 46179 email: celebrej@hhsc.ca This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. From rjbuesa <@t> yahoo.com Tue Jan 15 10:50:16 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 15 10:50:39 2008 Subject: [Histonet] Myth or Fact In-Reply-To: <0B3BF9D810C7094FA34B17DD145C3D5E056B96F8@ipemail01.hhsc.ca> Message-ID: <717827.52893.qm@web61223.mail.yahoo.com> That is a well documented fact (several articles in Histologic). Yes, you should dewax separatelu. Ren? J, Celebre Julia wrote: http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From MadaryJ <@t> MedImmune.com Tue Jan 15 10:50:16 2008 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Tue Jan 15 10:51:12 2008 Subject: [Histonet] Need the evg/evg procedure question below In-Reply-To: Message-ID: We moved and my old books sprouted legs. I want to do the elastic stain using the verhoeffs hema(the one that uses ferric chloride) and then differnetiates in 2% ferric chloride after that. I do not have resorcin or orcein and my aldehyde fuchsin is old so I am trying tio use what I know I have on hand to get this done now instead of waiting. I want to say evg/vvg. Nick Madary, HT/HTL(ASCP)QIHC Medimmune Histology Laboratory One Medimmune Way Gaithersburg, MD 20878 ph 301.398.4745/6360 fx 301.398.9745 To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, January 14, 2008 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 50, Issue 21 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Aurora (Pete and Sharon Jack) 2. Re: Who can perform Histology Duties (Esther Peters) 3. RBC removal (Min-Han Tan) 4. Re: BrdU/DCX problem (Olek Michalski) 5. RE: Who can perform Histology Duties (Marshall Terry Dr, Consultant Histopathologist) 6. RELIA Histology Opportunity Update 01-14-08 Happy New Year! (Pam Barker) ---------------------------------------------------------------------- Message: 1 Date: Sun, 13 Jan 2008 15:29:29 -0500 From: "Pete and Sharon Jack" Subject: [Histonet] Aurora To: Message-ID: <001101c85622$ffd90a70$90e84c47@pete38d56c4d11> Content-Type: text/plain; charset="iso-8859-1" Our laboratory was purchased by Auror on October 2, 2007. It's too soon to tell how things will be. We did lose our profit sharing which was quite lucrative. Sharon S. Jack, HT(ASCP) ------------------------------ Message: 2 Date: Sun, 13 Jan 2008 17:22:13 -0500 From: Esther Peters Subject: Re: [Histonet] Who can perform Histology Duties To: "Marshall Terry Dr, Consultant Histopathologist" Cc: histonet@lists.utsouthwestern.edu, Terri Braud , Kemlo Rogerson Message-ID: <478A8F15.5050904@verizon.net> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Terry, Enjoyed your comments. Training, continuing education, and especially knowing when you should refer to someone else are key in any profession! Regarding "norma,l" the pathologists are not using "some sort of mealy mouthed evasion" but they know that there is a "range" of normalcy when identifying the condition of cells and tissues, because of individual variation, age, reproductive status, nutritional status, etc. Just think of the wide range in values for what are considered "nondiseased" levels of various blood parameters. I once corresponded with a professor of histology, who told me he only knew "normal" and didn't know anything about pathology. But if you don't understand structural changes indicating diseases, are you sure you can recognize "normal"? Esther Peters, Ph.D. George Mason University Marshall Terry Dr, Consultant Histopathologist wrote: > "Proper training can allow Histotech's to report normal, ....." > > There was a bizarre discussion on the histopathology discussion group > recently, around reporting tissue as "normal". > An amazing number of pathologists never used the word, but preferred > some sort of mealy mouthed evasion. > Within normal limits, no definite lesion seen ... That sort of thing. > Indeed one very competent pathologist claimed he could not recognise > normal! > > Terry > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo > Rogerson > Sent: 10 January 2008 16:08 > To: Terri Braud; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Who can perform Histology Duties > > Please save your flames. The scenerio of a Non-Histotech embedding, > cutting, and staining my surgical tissue does not thrill me, but that > was not her question. Maybe the reality of the answer is why many > Histology Labs have always been considered the evil red-headed > stepchildren of the Lab. > Terri > > I think you are correct in that as long as someone is properly > trained, the training is revisited regularly and there is adequate > supervision and the professional groups are in agreement Non-BMS > (Non-Histotech) Staff can do many jobs in Histology and maybe Non > Medical Staff (BMS) can carry out some interpretation. I would have no > issues with a Non-Histotech (Non-BMS) doing as you say with proper > supervision by a HPC registered supervisor. > > But where does it end? Do you need a medical degree to identify vas, > appendix or gall bladder? You need the training to know when you have > reached the limits of your knowledge and when to refer to someone > better able to report a disease and I concede that is probably a > Medic. Proper training can allow Histotech's to report normal, maybe > non-normal requires medical insight, what do you think? > > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > Don't be afraid to take a big step when one is indicated. You can't > cross a chasm in two small jumps. --Buckminster Fuller > > This e-mail is confidential and privileged. If you are not the > intended recipient please accept my apologies; please do not disclose, > copy or distribute information in this e-mail or take any action in > reliance on its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ###################################################################### > This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. > The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. > If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. > ###################################################################### > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 3 Date: Mon, 14 Jan 2008 13:58:13 +0800 From: "Min-Han Tan" Subject: [Histonet] RBC removal To: histonet@lists.utsouthwestern.edu Message-ID: <7902152a0801132158x2be7da3dxb0649bd086c4f25d@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Dear fellow list members, I have been trying to remove RBCs from bloody pleural effusions / abdominal ascites using Histopaque, with low success, for the purpose of cytology. Histopaque works great on blood. I wonder if any one has successfully worked on bloody effusions / ascites previously; they do not seem to centrifuge in as optimal a fashion as blood, and I wonder if there are proteins / free hemoglobin that may be altering the density somewhat. Has anyone used ACK buffer or any other such methods to remove / lyse RBCs? Thanks! Regards, Min-Han Tan ------------------------------ Message: 4 Date: Mon, 14 Jan 2008 07:39:16 +0100 From: "Olek Michalski" Subject: Re: [Histonet] BrdU/DCX problem To: "Jonathan Frank" Cc: Histonet Message-ID: Content-Type: text/plain; format=flowed; delsp=yes; charset=iso-8859-2 Dear Jon, 2008-01-10 19:11:20 Jonathan Frank wrote: [...] > Thanks for the response. Do you do 1st primary, 1st secondary, then > brdU primary, and BrdU secondary? [...] It is exactly the case. If you like I can ask my coleague, who actually worked out the protocol and uses it routinely for a detailed list of opertions with some comments. At first glance I see some differences in BrdU labelling procedure. > Also, which BrdU primary antibody do you use? For BrdU we use antibody from Roche. I don't remember the catalogue no. and the characteristics I can found at the moment is a bit old but I suppose they offer only one kind. Best regards Olek -- Laboratory of Neurobiology of Development and Evolution Nencki Institute of Experimental Biology ul. Pasteura 3, 02-093 Warszawa, Poland Tel. +48 22 5892268, Fax +48 22 8225342 ------------------------------ Message: 5 Date: Mon, 14 Jan 2008 12:26:00 -0000 From: "Marshall Terry Dr, Consultant Histopathologist" Subject: RE: [Histonet] Who can perform Histology Duties To: "Esther Peters" Cc: histonet@lists.utsouthwestern.edu, Terri Braud , Kemlo Rogerson Message-ID: <5C0BED61F529364E86309CADEA63FEF2F3AF41@TRFT-EX01.xRothGen.nhs.uk> Content-Type: text/plain; charset="US-ASCII" Odd that there should be only one comment on my post, because I thought it (the subject rather than my post) was an interesting one. It's impossible to judge just what gets people interested. Anyway, back at the ranch, you are right that there is a range of appearances, but that range is only of significance in breast and prostate, of the things we see commonly, and it would certainly be true that "normal" has very fuzzy edges in these organs. However, for the rest, there is no significant range. Large bowel biopsies actually gives rise to problems too, as they are almost always abnormal, but only mildly so, and unfortunately, not showing any features by which one can make a diagnosis. I usually report these as no significant abnormality, or similar, but sometimes I just call them normal, as it really makes no practical difference. As these patients have bowel problems, usually diarrhoea, and many have had some sort of bowel prep., it is no surprise that the appearance should not be "normal" in the sense of ideal. It's all a matter of how you look at things, and in US in particular, how well you like to cover your ass. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Esther Peters Sent: 13 January 2008 22:22 To: Marshall Terry Dr, Consultant Histopathologist Cc: histonet@lists.utsouthwestern.edu; Terri Braud; Kemlo Rogerson Subject: Re: [Histonet] Who can perform Histology Duties Terry, Enjoyed your comments. Training, continuing education, and especially knowing when you should refer to someone else are key in any profession! Regarding "norma,l" the pathologists are not using "some sort of mealy mouthed evasion" but they know that there is a "range" of normalcy when identifying the condition of cells and tissues, because of individual variation, age, reproductive status, nutritional status, etc. Just think of the wide range in values for what are considered "nondiseased" levels of various blood parameters. I once corresponded with a professor of histology, who told me he only knew "normal" and didn't know anything about pathology. But if you don't understand structural changes indicating diseases, are you sure you can recognize "normal"? Esther Peters, Ph.D. George Mason University Marshall Terry Dr, Consultant Histopathologist wrote: > "Proper training can allow Histotech's to report normal, ....." > > There was a bizarre discussion on the histopathology discussion group > recently, around reporting tissue as "normal". > An amazing number of pathologists never used the word, but preferred > some sort of mealy mouthed evasion. > Within normal limits, no definite lesion seen ... That sort of thing. > Indeed one very competent pathologist claimed he could not recognise > normal! > > Terry > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo > Rogerson > Sent: 10 January 2008 16:08 > To: Terri Braud; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Who can perform Histology Duties > > Please save your flames. The scenerio of a Non-Histotech embedding, > cutting, and staining my surgical tissue does not thrill me, but that > was not her question. Maybe the reality of the answer is why many > Histology Labs have always been considered the evil red-headed > stepchildren of the Lab. > Terri > > I think you are correct in that as long as someone is properly > trained, the training is revisited regularly and there is adequate > supervision and the professional groups are in agreement Non-BMS > (Non-Histotech) Staff can do many jobs in Histology and maybe Non > Medical Staff (BMS) can carry out some interpretation. I would have no > issues with a Non-Histotech (Non-BMS) doing as you say with proper > supervision by a HPC registered supervisor. > > But where does it end? Do you need a medical degree to identify vas, > appendix or gall bladder? You need the training to know when you have > reached the limits of your knowledge and when to refer to someone > better able to report a disease and I concede that is probably a > Medic. Proper training can allow Histotech's to report normal, maybe > non-normal requires medical insight, what do you think? > > > Kemlo Rogerson > Pathology Manager > DD 01934 647057 or extension 3311 > Mob 07749 754194; Pager 07659 597107; > Don't be afraid to take a big step when one is indicated. You can't > cross a chasm in two small jumps. --Buckminster Fuller > > This e-mail is confidential and privileged. If you are not the > intended recipient please accept my apologies; please do not disclose, > copy or distribute information in this e-mail or take any action in > reliance on its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ###################################################################### > This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. > The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. > If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. > ###################################################################### > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Mon, 14 Jan 2008 09:01:10 -0500 From: "Pam Barker" Subject: [Histonet] RELIA Histology Opportunity Update 01-14-08 Happy New Year! To: "'Histonet'" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters! What's your New Year's Resolution? Of course I will try to eat right and exercise. But... My Resolution is to help even more histo techs find the right opportunity in the right place at the right time. In 2007 I helped people make the move into a management role, relocate closer to family, shorten their commute, move into research, utilize their advanced degrees, get better salaries, shifts and benefits. In 2008 I resolve to help even more people make the changes they want to make in order to improve their career situations and ultimately their quality of life. Whatever you want to do Wherever you want to be. Let Me Help! Take Advantage of RELIA's Career Coaching Program. Free of Charge RELIA offers: * Assistance with updating or creating your resume * Tips on interviewing * Encouragement and Assistance during the course of your job search * Responsiveness - I will respond to your e-mails/phone calls within 24 hours or less. * Troubleshooting - if your job search is stalled or you can't get in the company you are interested in. * Personalized Job Search - customized to your experience, wants and needs. * Complete Confidentiality - Your career and my coaching is a relationship of trust. I have a nationwide network of contacts with the premier employers of histology professionals in clinical, research and biotech settings. I only work with full time permanent positions at companies that offer excellent compensation, benefits programs and relocation assistance. Here are some of my current openings: Histology Managers/Supervisors/Specialists needed in: Dallas, TX - Clinical Los Angeles, CA - Clinical Seattle, WA - Research/Clinical Waco, TX - Clinical Immunohistochemistry Histotechnicians/Histotechnologists needed in: Phoenix, AZ - Clinical Los Angeles, CA - Clinical Los Angeles, CA - oppty to learn MOHS Orlando, FL - Dermatopathology Pittsburgh, PA - Clinical Cincinatti, OH - Clinical Austin, TX - Clinical Waco, TX - Clinical Concord, NH - Clinical Seattle, WA - Research/Clinical Helena, MT - Clinical "Heads Up" These are areas where some of my clients project a need in the near future. Indianapolis, IN Ft. Lauderdale, FL Boston, MA Ann Arbor, MI Rhode Island If you or any of your friends would like more information on RELIA'S Career Coaching or about any of the positions listed please contact me. I would be more than happy to assist you. You can reach me at 866-607-3542 or relia1@earthlink.net Remember it never hurts to look... Hope to hear from you soon . Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.myspace.com/pamatrelia ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 50, Issue 21 **************************************** From Rcartun <@t> harthosp.org Tue Jan 15 10:55:48 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Jan 15 10:56:24 2008 Subject: [Histonet] WT-1 In-Reply-To: References: Message-ID: <478C9F44020000770000A2BE@gwmail4.harthosp.org> I have had good results recently with Leica-Microsystems' (formerly Vision Biosystems/Novocastra) antibody (clone WT49); however, I use it on the Bond Max. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Sebree Linda A." 01/15/08 11:37 AM >>> Good Morning everyone, I was wondering what people are using for WT-1 (Wilm's tumor) antibody. I'm especially interested in this antibody being used with Ventana instrumentation. Thanks, Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From settembr <@t> umdnj.edu Tue Jan 15 11:12:57 2008 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Tue Jan 15 11:14:02 2008 Subject: [Histonet] WT-1 Message-ID: I use Dako's on the Dako Autostainer. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> "Sebree Linda A." 01/15/08 11:37 AM >>> Good Morning everyone, I was wondering what people are using for WT-1 (Wilm's tumor) antibody. I'm especially interested in this antibody being used with Ventana instrumentation. Thanks, Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Tue Jan 15 12:16:46 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Jan 15 12:17:15 2008 Subject: [Histonet] WT-1 In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C867@IS-E2K3.grhs.net> I have been using Cell Marque's WT-1, clone 6F-H2 on our Ventana with great results. They even have a protocol they can give you if you ask. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A. Sent: Tuesday, January 15, 2008 10:38 AM To: Histonet Subject: [Histonet] WT-1 Good Morning everyone, I was wondering what people are using for WT-1 (Wilm's tumor) antibody. I'm especially interested in this antibody being used with Ventana instrumentation. Thanks, Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jdmd77 <@t> hotmail.com Tue Jan 15 13:40:22 2008 From: jdmd77 <@t> hotmail.com (Julia Dahl) Date: Tue Jan 15 13:40:46 2008 Subject: [Histonet] Aurora In-Reply-To: <001101c85622$ffd90a70$90e84c47@pete38d56c4d11> References: <001101c85622$ffd90a70$90e84c47@pete38d56c4d11> Message-ID: Interesting. It isn't surprising that the model of acquisition that Mr. New continues to recycle segregates profits to the shareholders (the suits at the top) and eliminates profit sharing for those in the trenches taking care of the patients. > From: babears@triad.rr.com > To: histonet@lists.utsouthwestern.edu > Date: Sun, 13 Jan 2008 15:29:29 -0500 > Subject: [Histonet] Aurora > > Our laboratory was purchased by Auror on October 2, 2007. > It's too soon to tell how things will be. We did lose our profit sharing which was quite lucrative. > Sharon S. Jack, HT(ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Watch ?Cause Effect,? a show about real people making a real difference. http://im.live.com/Messenger/IM/MTV/?source=text_watchcause From Shirley_PHUA <@t> hsa.gov.sg Tue Jan 15 14:01:43 2008 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Tue Jan 15 14:02:13 2008 Subject: [Histonet] Shirley Phua is out-of-office ... Message-ID: I will be out of the office from 15-01-2008 to 16-01-2008. I'll be out-of-office on 15 January 2008. Pathologists: I will process your requests when I return. If urgent, please forward your email to Henry_Kyaw@hsa.gov.sg From jennifer.harvey <@t> Vanderbilt.Edu Tue Jan 15 14:11:21 2008 From: jennifer.harvey <@t> Vanderbilt.Edu (Harvey, Jennifer Lynn) Date: Tue Jan 15 14:11:44 2008 Subject: [Histonet] no answer at biomeda Message-ID: Hi, Has anyone hear what is going on at Biomeda? I order something in December through Fisher from them and still don't have it. I tried to call Biomeda and it rings busy and the "new numbers" just ring and ring. We also have emailed and nothing. They had storms last week that could be the problem. Thanks Jennifer Harvey, HT(ASCP) QIHC Vanderbilt Vision Research Center RM 8105 MCE North Tower 1215 21st Ave. South Nashville, TN 37232-8808 Phone: 615-936-1486 From PatPatterson <@t> mhd.com Tue Jan 15 14:17:32 2008 From: PatPatterson <@t> mhd.com (Patterson, Patricia) Date: Tue Jan 15 14:18:09 2008 Subject: [Histonet] IHC for IgG4 Message-ID: We currently have a formalin fixed tissue sample from a patient case suspecting Autoimmune Pancreatitis and current articles suggest IHC with IgG4 antibody. I've tried the usual (and some unusual) reference labs to see if anyone is offering this IHC test - so far no luck. We're also trying to find is there is a lab (maybe more research based) willing to do a consultation on this tissue sample. Does anyone have any ideas? Pat Patterson Methodist Dallas Medical Center 214-947-3538 patpatterson@mhd.com *********************************************************************** This electronic transmission contains information from Methodist Health System and should be considered confidential and privileged. The information contained in the above messages is intended only for the use of the individual(s) and entity(ies) named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution, or use of this information is prohibited. If you receive this transmission in error, please notify the sender immediately by return e-mail. Methodist Health System, its subsidiaries and affiliates hereby claim all applicable privileges related to the transmission of this communication. From anitathorn <@t> comcast.net Tue Jan 15 15:04:14 2008 From: anitathorn <@t> comcast.net (anitathorn@comcast.net) Date: Tue Jan 15 15:04:39 2008 Subject: [Histonet] block filing cabinets Message-ID: <011520082104.8507.478D1FCE000B991A0000213B2200750330029D01089B0E9B07020E@comcast.net> We use Lab Storage Systems for our blocks. Cat. # CB-2-1 Their telephone # is 1-800-345-4167. They also have a slide filing system but we've never used it because we keep recycling our old green metal cabinets! Hope this helps. -------------- Original message -------------- From: "Sharon.Davis-Devine" > The company that we have always bought our filing cabinets from for our > slide and block filing no longer sells them can some of you point me in > the direction of a different company? Also we have limited space as > most labs do so how many blocks and slides do you store onsite? Any help > or suggestions are greatly appreciated. Thanks. > > > > Sharon Davis-Devine, CT (ASCP) > > Cytology Supervisor > > Carle Clinic > > 602 West University > > Urbana, Illinois 61801 > > Phone: 217-383-3572 > > Email: sharon.davis-devine@carle.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pinetree110567 <@t> yahoo.com Tue Jan 15 15:36:46 2008 From: pinetree110567 <@t> yahoo.com (Jack Cates) Date: Tue Jan 15 15:37:09 2008 Subject: [Histonet] Re: block filing cabinets Message-ID: <516703.95675.qm@web44908.mail.sp1.yahoo.com> We use the storage system from Raymond Lamb, we get them through PSL in California. Nice system! fits together really well and I like the replacement drawers for our longterm storage, allows us to only buy the "cabinet" part once and it comes with a sturdy box for storage. Also, I think LabStorage has them as well and they may be closer to the midwest. Message: 7 Date: Tue, 15 Jan 2008 08:29:13 -0600 From: "Sharon.Davis-Devine" Subject: [Histonet] block filing cabinets To: Message-ID: <44780C571F28624DBB446DE55C4D733A021E084C@EXCHANGEBE1.carle.com> Content-Type: text/plain; charset="us-ascii" The company that we have always bought our filing cabinets from for our slide and block filing no longer sells them can some of you point me in the direction of a different company? Also we have limited space as most labs do so how many blocks and slides do you store onsite? Any help or suggestions are greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com ____________________________________________________________________________________ Looking for last minute shopping deals? Find them fast with Yahoo! Search. http://tools.search.yahoo.com/newsearch/category.php?category=shopping From jblaine <@t> bio-search.com Tue Jan 15 16:16:17 2008 From: jblaine <@t> bio-search.com (Jason Blaine) Date: Tue Jan 15 16:16:41 2008 Subject: [Histonet] Histotech Opportunity Available @ NIH as a Contractor Message-ID: <045401c857c4$4010aba0$046fa8c0@Team.teamplace.com> Position Overview: The position is a Histotechnician in the Core Histology Lab within the National Eye Institute (NEI) at NIH. Because all of the cases are research focused this a low volume laboratory with an emphasis on high quality. They are willing to train on working with the eye but need someone with basic Histology skills. The job is on the main campus of NIH in Bethesda. The position is Monday through Friday from 8:30AM --> 5PM or 9AM to 5:30PM. Qualifications: 1 to 3 years of general Histotech experience including working with paraffin and frozens in required. Ability and willingness to learn to work with methacrylate is required. HT eligibility is highly desired. Prior manual IHC experience is a plus. Qualified candidates please submit a current and thorough resume/CV to jblaine@bio-search.com Thanks - Jason Blaine Director Tel: 301-565-3908 ext. 301 Cell: 301-219-3379 Fax: 301-565-3915 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ BioSearch a division of Team Placement Service, Inc. From tjey <@t> hotmail.com Tue Jan 15 20:17:26 2008 From: tjey <@t> hotmail.com (Tanya Ewing-Finchem) Date: Tue Jan 15 20:17:51 2008 Subject: [Histonet] Teaching Opportunity Message-ID: Great Teaching Opportunity in Sunny Warm Tucson Arizona. Pima Community College is advertising for Director of the Histo Technology Program. You need a BS or above and HT/HTL. Great place to build a strong program for new histologists. You can see the posting on their web page: http://www.pima.edu/pimajobs/ under staff positions. Note that the salary is posted for a 9 month position, but it is a year round program and the pay would be more and is also based on your experience. From Andrew.Prior <@t> Smith-Nephew.com Wed Jan 16 02:22:41 2008 From: Andrew.Prior <@t> Smith-Nephew.com (Prior, Andrew) Date: Wed Jan 16 02:23:14 2008 Subject: [Histonet] Re: block filing cabinets Message-ID: <7028DD15E14FDC4DB1F3C5AF8735AF3704ECC5B1@EHS021.wound.san> We use the block and slide storage drawers from R.A. Lamb. Nice (but basic) metal drawer units that stack easily. We keep at least the last 3 years worth of slides, but probably have less volume than most labs as we're a research lab. Andrew Andrew Prior Histologist Smith &Nephew Research Centre York Science Park Heslington York YO10 5DF UK Andrew.Prior@smith-nephew.com 01904 824022 >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> Message: 7 Date: Tue, 15 Jan 2008 08:29:13 -0600 From: "Sharon.Davis-Devine" Subject: [Histonet] block filing cabinets To: Message-ID: <44780C571F28624DBB446DE55C4D733A021E084C@EXCHANGEBE1.carle.com> Content-Type: text/plain; charset="us-ascii" The company that we have always bought our filing cabinets from for our slide and block filing no longer sells them can some of you point me in the direction of a different company? Also we have limited space as most labs do so how many blocks and slides do you store onsite? Any help or suggestions are greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com Confidentiality. This electronic transmission is strictly confidential to Smith & Nephew and intended solely for the addressee. It may contain information which is covered by legal, professional or other privilege. If you are not the intended addressee, or someone authorised by the intended addressee to receive transmissions on behalf of the addressee, you must not retain, disclose in any form, copy or take any action in reliance on this transmission. If you have received this transmission in error, please notify the sender as soon as possible and destroy this message. Smith & Nephew UK Limited Registered in England and Wales No.4421171 with registered office at 15 Adam Street, London WC2N 6LA From kb4091 <@t> yahoo.com Wed Jan 16 04:24:51 2008 From: kb4091 <@t> yahoo.com (Kathy Munro) Date: Wed Jan 16 04:25:15 2008 Subject: [Histonet] Histotech needed!! Message-ID: <396012.73200.qm@web39610.mail.mud.yahoo.com> Needed , a registered histology technician. If you know immunos (we are automated, Vision Biosystems) that is a big plus! We use the Cerner computer system. M-F shift Near the beach Low cost of living Small lab. 2 techs, 6000 surgicals a year Please contact Audrey McClaine or Human Resources if interested Nanticoke Memorial Hospital 801 Middleford Road Seaford, De 19973 (302)-629-6611 ____________________________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ From mulpiece <@t> yahoo.com Wed Jan 16 06:25:53 2008 From: mulpiece <@t> yahoo.com (Michael Muller) Date: Wed Jan 16 06:26:14 2008 Subject: [Histonet] WT-1 on Ventana Message-ID: <267583.4463.qm@web54108.mail.re2.yahoo.com> I use the prediluted ab from Cell Marque on my Ventana Benchmark XT with the ultra-view kit. Stain works great. Michael Muller HTL ASCP Converge Diagnostics Peabody MA ____________________________________________________________________________________ Never miss a thing. Make Yahoo your home page. http://www.yahoo.com/r/hs From rhessler <@t> pathgroup.com Wed Jan 16 07:57:41 2008 From: rhessler <@t> pathgroup.com (Richard Hessler, M.D.) Date: Wed Jan 16 07:58:05 2008 Subject: [Histonet] AE1/AE3 cross reactivity Message-ID: <197CD0B02A81F94994A285C59C8AE05C023B29AE7C@pgnexchange.pathgroup.com> I have used this antibody for years but now have issues. Previously it was the best choice for breast sentinel nodes, but now AE1/AE3 stains plasma cells like CD138. It also stains a number of different sarcomas and beautifully highlights reactive astrocytes. I had previously used Dako Ab and autostainer with no problems. Now I have Ventanna and have noticed this with both Ventana and Dako Abs used on that system. Any thoughts??? Richard B Hessler, MD Chief of Pathology Erlanger Medical Center Chattanooga, TN ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From Dolores_Fischer <@t> baxter.com Wed Jan 16 08:24:29 2008 From: Dolores_Fischer <@t> baxter.com (Dolores_Fischer@baxter.com) Date: Wed Jan 16 08:35:49 2008 Subject: [Histonet] complement C3, C4 Message-ID: Good morning everyone! I was asked to work up a protocol for the detection of C3 and C4 in rodent tissue. Are there immunohistochemical procedures for this? Quickly scanning for information I found an antibody that is for Elisa applications. Any guidance from all of you immuno experts out there would be much appreciated! Thanks! Dolores The information transmitted is intended only for the person(s)or entity to which it is addressed and may contain confidential and/or legally privileged material. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive privilege or confidentiality. Any review, retransmission, dissemination or other use of , or taking of any action in reliance upon, this information by entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. For Translation: http://www.baxter.com/email_disclaimer From Rcartun <@t> harthosp.org Wed Jan 16 09:17:09 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Jan 16 09:17:42 2008 Subject: [Histonet] IHC for IgG4 In-Reply-To: References: Message-ID: <478DD9A5020000770000A309@gwmail4.harthosp.org> We recently sent a case for consultation to Dr. Gregory Lauwers at Massachusetts General Hospital (617-726-2967). Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Patterson, Patricia" 01/15/08 3:17 PM >>> We currently have a formalin fixed tissue sample from a patient case suspecting Autoimmune Pancreatitis and current articles suggest IHC with IgG4 antibody. I've tried the usual (and some unusual) reference labs to see if anyone is offering this IHC test - so far no luck. We're also trying to find is there is a lab (maybe more research based) willing to do a consultation on this tissue sample. Does anyone have any ideas? Pat Patterson Methodist Dallas Medical Center 214-947-3538 patpatterson@mhd.com *********************************************************************** This electronic transmission contains information from Methodist Health System and should be considered confidential and privileged. The information contained in the above messages is intended only for the use of the individual(s) and entity(ies) named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution, or use of this information is prohibited. If you receive this transmission in error, please notify the sender immediately by return e-mail. Methodist Health System, its subsidiaries and affiliates hereby claim all applicable privileges related to the transmission of this communication. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From twheelock <@t> mclean.harvard.edu Wed Jan 16 10:08:58 2008 From: twheelock <@t> mclean.harvard.edu (Tim Wheelock) Date: Wed Jan 16 09:22:51 2008 Subject: [Histonet] PLEASE UNSUBSCRIBE ME Message-ID: <478E2C1A.6000909@mclean.harvard.edu> Please unsubscribe me. Thank you. The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From john.ricci <@t> nyu.edu Wed Jan 16 09:36:05 2008 From: john.ricci <@t> nyu.edu (John L Ricci) Date: Wed Jan 16 09:36:28 2008 Subject: [Histonet] Please unsubscribe me Message-ID: Please unsubscribe me from your list Thanks, John Ricci From jcokie <@t> yahoo.com Wed Jan 16 10:28:11 2008 From: jcokie <@t> yahoo.com (Jeanenne Ely) Date: Wed Jan 16 10:28:35 2008 Subject: [Histonet] Job opening in Houston Message-ID: <15210.22301.qm@web81404.mail.mud.yahoo.com> Hello all- We have an opening for a Senior Research Histology Tech at MD Anderson Cancer Center, Veterinary Medicine and Research Department. The salary range is from $33,600 to $50,400 per year. The complete position description may be accessed at www.mdanderson.org. You may also apply online. --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From laurie.colbert <@t> huntingtonhospital.com Wed Jan 16 11:06:24 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed Jan 16 11:06:45 2008 Subject: [Histonet] CJD Decontamination Message-ID: <57BE698966D5C54EAE8612E8941D76830248A47C@EXCHANGE3.huntingtonhospital.com> I know this has been discussed previously, but I'm getting conflicting information. I am trying to find a procedure on decontaminating unbroken skin. Several sources say to use either 1N or 2N sodium hydroxide, but info from WHO says to use a 0.1N solution of sodium hydroxide. Any comments?? Laurie Colbert From bwhitaker <@t> brownpathology.com Wed Jan 16 11:26:04 2008 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Wed Jan 16 11:21:55 2008 Subject: [Histonet] meeting announcement Message-ID: <000001c85864$e025d9d0$3601a8c0@brownpathology.net> Hi Everyone, For anyone within the greater Houston, TX area, or willing to travel to Houston for CEUs, District II of the Texas Society for Histotechnology would like to announce an upcoming event: McCormick Scientific and StatLab Medical Products are sponsoring a meeting on Saturday, February 9 from 9:00am to 2:00pm to be held at: University of Texas M.D. Anderson Cancer Center School of Health Sciences Houston Main Building--Room HMB 4.400 1100 Holcombe Blvd. Houston, TX 77030 There will be three lectures included: Basic Fundamentals of Processing, including Reprocessing of Breast Tissue, Cost and Workload Value The New Regulatory Standards (CAP) in Her2 Studies The Paradox of Change in Histotechnology There will be no charge, but you MUST RSVP for confirmed seating and lunch by no later than Wednesday, Feb. 6, 2008 Call: Callie Stapleton at 866-737-2540 (toll free) or Email: Skip Brown at sbrown@mccormickscientific.com We look forward to seeing you there!! Bonnie Whitaker District II Director Texas Society for Histotechnology From lelmgren <@t> sunriselab.com Wed Jan 16 11:42:47 2008 From: lelmgren <@t> sunriselab.com (Laurie Elmgren) Date: Wed Jan 16 11:43:24 2008 Subject: [Histonet] Nuclear Staining on Prostate Biopsies Message-ID: <4A672C6AE0402D4A89ECE29E8A4B47E32B7570@MailPDC.sunriselab.com> I would like to optimize the quality of our stain. We process office biopsies, a good variety of tissue types. The stain is consistently very good. There are three colors of Eosin, and good nuclear detail on most tissue types, except for the prostate cores. The nuclei are stained dark and are "flat" having no depth. I am thinking that it is not the fixation, because our Monday specimens that are held over a full day are no different than those throughout the week. Any suggestions before I start reprogramming the stainer? Laurie Elmgren Histology Supervisor Sunrise Medical Labs 240 Motor Pkwy Hauppauge, NY 11788 (631)435-1515-x1108 From rjbuesa <@t> yahoo.com Wed Jan 16 11:57:22 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 16 11:57:45 2008 Subject: [Histonet] CJD Decontamination In-Reply-To: <57BE698966D5C54EAE8612E8941D76830248A47C@EXCHANGE3.huntingtonhospital.com> Message-ID: <168188.72314.qm@web61216.mail.yahoo.com> I don't know about the 0.1N BUT for sure you will get skin damage with either 1N or 2N sodium hydroxide. Ren? J. Laurie Colbert wrote: --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From rjbuesa <@t> yahoo.com Wed Jan 16 11:59:05 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 16 11:59:27 2008 Subject: [Histonet] Nuclear Staining on Prostate Biopsies In-Reply-To: <4A672C6AE0402D4A89ECE29E8A4B47E32B7570@MailPDC.sunriselab.com> Message-ID: <134726.71724.qm@web61223.mail.yahoo.com> Check your processing schedule, could be too long for the prostate Bx, or too much time in alcohols. Ren? J. Laurie Elmgren wrote: --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From laurie.colbert <@t> huntingtonhospital.com Wed Jan 16 12:17:30 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed Jan 16 12:17:54 2008 Subject: [Histonet] Nuclear Staining on Prostate Biopsies Message-ID: <57BE698966D5C54EAE8612E8941D76830248A4B6@EXCHANGE3.huntingtonhospital.com> One of the biggest problems that we encounter is that the prostate biopsies are drying out at the office before they get put into the formalin. One office in particular places the specimens on gauze before they go into formalin. I think they obtain all of the specimens and then put them all into the bottles of formalin last. We get bad staining, crunchy, dry specimens, etc. If you are having problems with a particular office, talk to them and make sure the tissue is going into formalin immediately after it is removed from the patient. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Elmgren Sent: Wednesday, January 16, 2008 9:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nuclear Staining on Prostate Biopsies I would like to optimize the quality of our stain. We process office biopsies, a good variety of tissue types. The stain is consistently very good. There are three colors of Eosin, and good nuclear detail on most tissue types, except for the prostate cores. The nuclei are stained dark and are "flat" having no depth. I am thinking that it is not the fixation, because our Monday specimens that are held over a full day are no different than those throughout the week. Any suggestions before I start reprogramming the stainer? Laurie Elmgren Histology Supervisor Sunrise Medical Labs 240 Motor Pkwy Hauppauge, NY 11788 (631)435-1515-x1108 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vsnider <@t> shrinenet.org Wed Jan 16 12:17:56 2008 From: vsnider <@t> shrinenet.org (Snider, Vivian Deanna) Date: Wed Jan 16 12:18:19 2008 Subject: [Histonet] no answer at Biomeda Message-ID: <84BE46B37B314D409C5A17B7BAB022D601BF9C21@IDC-EX-VS01.shriners.cc> I have been having some issues with Biomeda also. It has been almost a month and I still have not received the product, ordered through Fisher. I am told it is a manufacturer's backorder. I am currently researching a secondary source for the product. Deanna Snider HT ASCP Lead Histotechnician Shriners' Hospital for Children Research Dept. Cincinnati, OH CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients. If you are not the intended recipient, (or authorized to receive for the recipient) you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please destroy all copies of this communication and any attachments and contact the sender by reply e-mail or telephone (813) 281-0300. From TJJ <@t> Stowers-Institute.org Wed Jan 16 12:56:20 2008 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Wed Jan 16 12:57:08 2008 Subject: [Histonet] RE: Nuclear staining on prostate biopsies In-Reply-To: Message-ID: Laurie, contact the operating room (or doctor's office) staff and ask them how the samples are handled when they are released from the biopsy gun. My guess is they are put on dry gauze and some air drying is taking place prior to being fixed. Or they are placed in some other medium besides fixative immediately. Many times the damage is already done before we ever receive the specimen. It's just a guess on my part, but mostly I think it's because all the other tissue types look good with your processing and staining times. Good luck! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 >>Laurie wrote: I would like to optimize the quality of our stain. We process office biopsies, a good variety of tissue types. The stain is consistently very good. There are three colors of Eosin, and good nuclear detail on most tissue types, except for the prostate cores. The nuclei are stained dark and are "flat" having no depth. I am thinking that it is not the fixation, because our Monday specimens that are held over a full day are no different than those throughout the week. Any suggestions before I start reprogramming the stainer? Laurie Elmgren Histology Supervisor Sunrise Medical Labs 240 Motor Pkwy Hauppauge, NY 11788 (631)435-1515-x1108 From mtarango <@t> nvcancer.org Wed Jan 16 14:45:14 2008 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Wed Jan 16 14:45:52 2008 Subject: [Histonet] AE1/AE3 cross reactivity In-Reply-To: <197CD0B02A81F94994A285C59C8AE05C023B29AE7C@pgnexchange.pathgroup.com> Message-ID: <5AEC610C1CE02945BD63A395BA763EDE01E48CDE@NVCIEXCH02.NVCI.org> I noticed the plasma cell staining just yesterday. We use Ventana's pancytokeratin cocktail (AE1/AE3/PCK26). I was using LU-5 for pankeratin previously, but had problems with that antibody (no staining). Mark Adam Tarango HT(ASCP)QIHC Histology & IHC Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 Direct Line (702) 822-5112 Treo (702) 759-9229 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Hessler, M.D. Sent: Wednesday, January 16, 2008 5:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AE1/AE3 cross reactivity I have used this antibody for years but now have issues. Previously it was the best choice for breast sentinel nodes, but now AE1/AE3 stains plasma cells like CD138. It also stains a number of different sarcomas and beautifully highlights reactive astrocytes. I had previously used Dako Ab and autostainer with no problems. Now I have Ventanna and have noticed this with both Ventana and Dako Abs used on that system. Any thoughts??? Richard B Hessler, MD Chief of Pathology Erlanger Medical Center Chattanooga, TN ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From sprice2003 <@t> gmail.com Wed Jan 16 16:52:30 2008 From: sprice2003 <@t> gmail.com (Sally Price) Date: Wed Jan 16 16:52:53 2008 Subject: [Histonet] AE1/AE3 cross reactivity Message-ID: Dr. Hessler: I have observed the phenomenon that you've described for other antibodies more times than I can remember. Personally, I believe that it has to do with the way the Ventana system sprays on the retrieval solution and heats slides during antibody/detection incubation periods, which is too aggressive. I think that an open system that doesn't heat slides or perform AR isn't likely to produce these unexpalined reactions, but I digress. So, how do you fix the problem? I say disable the on-line AR, and perform it in a decloaking chamber, set at 100 degrees for 10 minutes. Or, you could consider using another pan-CK antibody, like Lu-5. Good Luck, Sally Price ---------------------------------------------------------------------------------------------- Date: Wed, 16 Jan 2008 07:57:41 -0600 From: "Richard Hessler, M.D." Subject: [Histonet] AE1/AE3 cross reactivity To: "histonet@lists.utsouthwestern.edu" " I have used this antibody for years but now have issues. Previously it was the best choice for breast sentinel nodes, but now AE1/AE3 stains plasma cells like CD138. It also stains a number of different sarcomas and beautifully highlights reactive astrocytes. I had previously used Dako Ab and autostainer with no problems. Now I have Ventanna and have noticed this with both Ventana and Dako Abs used on that system. Any thoughts??? Richard B Hessler, MD Chief of Pathology Erlanger Medical Center Chattanooga, TN From djemge <@t> aol.com Wed Jan 16 17:54:45 2008 From: djemge <@t> aol.com (djemge@aol.com) Date: Wed Jan 16 17:55:26 2008 Subject: [Histonet] re: Nuclear Staining on Prostate Biopsies Message-ID: <8CA26B24E3B1125-8AC-4B4@MBLK-M08.sysops.aol.com> This dark nuclei is often seen when tissue is left to dry for a few minutes before being fixed, or?if underfixed and?the alcohols on the processor fix the tissue. Since you are sure it is not dried or underfixed then it has to be the processing schedule. I agree with Renee. This sounds like a processing problem. Sounds like the overall schedule is too long for these biopsies. Especially if too long in paraffin. Donna Donna Emge HT-ASCP Northwestern University Feinburg School of Medicine 303 E. Superior, Lurie 7-220 Chicago, IL 60611 312-503-2036 d-emge@northwestern.edu ________________________________________________________________________ More new features than ever. Check out the new AOL Mail ! - http://webmail.aol.com From jnocito <@t> satx.rr.com Wed Jan 16 17:58:28 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Jan 16 17:59:47 2008 Subject: [Histonet] Nuclear Staining on Prostate Biopsies References: <4A672C6AE0402D4A89ECE29E8A4B47E32B7570@MailPDC.sunriselab.com> Message-ID: <004601c8589b$c949c7d0$0302a8c0@yourxhtr8hvc4p> Laurie, at my last place, we had to set up a different staining program for the reasons you stated. We decreased the hematoxylin and differentiation times, increased the bluing time and increased the wash time. I set up testing as follows: Group 1: hema at 30 second intervals 2:00, 2:30, 3:00, 3:30 , everything else stayed the same (4 slides) Group 2 : hema at 30 second intervals , but increase bluing 15 second intervals 30, 45, 60, 75 seconds, everything else stayed the same (16 slides) Group 3: hema 30 second intervals, bluing 15 second intervals and differentiation time by 15 seconds (64 slides, I think) Lot of work, no doubt, but that's what the pathologists wanted. Have fun and good luck JTT ----- Original Message ----- From: "Laurie Elmgren" To: Sent: Wednesday, January 16, 2008 11:42 AM Subject: [Histonet] Nuclear Staining on Prostate Biopsies I would like to optimize the quality of our stain. We process office biopsies, a good variety of tissue types. The stain is consistently very good. There are three colors of Eosin, and good nuclear detail on most tissue types, except for the prostate cores. The nuclei are stained dark and are "flat" having no depth. I am thinking that it is not the fixation, because our Monday specimens that are held over a full day are no different than those throughout the week. Any suggestions before I start reprogramming the stainer? Laurie Elmgren Histology Supervisor Sunrise Medical Labs 240 Motor Pkwy Hauppauge, NY 11788 (631)435-1515-x1108 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Jan 17 02:51:20 2008 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Jan 17 02:51:50 2008 Subject: [Histonet] Not negative Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F4BB@wahtntex2.waht.swest.nhs.uk> I think we had a discussion last week about Pathologists reporting negative slides; interestingly there's a television news story that just broken about a Welsh Pathologist wrongly reporting biopsies. I gather there are a number of biopsies that were initially reported as negative in which the Patients subsequently developed cancer; or that's what I think the story said. This Pathologists work over a number of years is to be reviewed; reminds me of the bad years in Cytology. Abnormal slides are peer reviewed but not 'negative' ones which is odd; if you diagnosed something then the Patient will be treated (or further investigated which might flag up errors), if you call something negative then the Patient may have no treatment unless the symptoms remain and further tests reveal the abnormality. How gold is the gold standard of tissue diagnosis? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Don't be afraid to take a big step when one is indicated. You can't cross a chasm in two small jumps. --Buckminster Fuller This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From louise.renton <@t> gmail.com Thu Jan 17 05:37:20 2008 From: louise.renton <@t> gmail.com (louise renton) Date: Thu Jan 17 05:37:48 2008 Subject: [Histonet] bone picture Message-ID: Hi all, is anyone willing to share a low power view of an undecalcified MMA bone section?? I desperately need one for a talk I am giving and our camera has gone all funny and won't take low power pictures...any help at all, please????? best regards- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From rjbuesa <@t> yahoo.com Thu Jan 17 08:19:34 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 17 08:19:57 2008 Subject: [Histonet] Not negative In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF0222F4BB@wahtntex2.waht.swest.nhs.uk> Message-ID: <540032.28982.qm@web61221.mail.yahoo.com> This reinforces my view that a "false negative" is always worse that a "false positive" although some pathlogists disagree. With a "false negative" you do nothing and with a "false positive" you do something even if it is not necessary, you err in the "cautious side". Ren? J. Kemlo Rogerson wrote: --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From sotlak <@t> yahoo.gr Thu Jan 17 08:51:58 2008 From: sotlak <@t> yahoo.gr (sotiris lakis) Date: Thu Jan 17 08:52:25 2008 Subject: [Histonet] Hepsin immunohistochemistry Message-ID: <455401.58701.qm@web23001.mail.ird.yahoo.com> Hello everybody, I am interested in performing immunohistochemistry with hepsin on paraffin sections from prostate carcinoma tissue. I would be greatful if somebody who has already tested any of the antibodies commercially availiable would give me a hint about what to expect. Thank you in advance Sotiris Lakis --------------------------------- ?????????????? Yahoo! ?????????? ?? ?????????? ???? ???? (spam); ?? Yahoo! Mail ???????? ??? ???????? ?????? ????????? ???? ??? ??????????? ????????? http://login.yahoo.com/config/mail?.intl=gr From bakevictoria <@t> gmail.com Thu Jan 17 08:53:05 2008 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Thu Jan 17 08:53:28 2008 Subject: [Histonet] no answer at Biomeda In-Reply-To: <84BE46B37B314D409C5A17B7BAB022D601BF9C21@IDC-EX-VS01.shriners.cc> References: <84BE46B37B314D409C5A17B7BAB022D601BF9C21@IDC-EX-VS01.shriners.cc> Message-ID: <4f016b690801170653s605768c1q77874a796f6a683f@mail.gmail.com> I had a situation a couple of years back where I was purchasing supplies through Fisher and also experienced LONG waits for receipt of products. It turned out that in some instances Fisher has to go through another distributor as the direct manufacturer will not sell direct to Fisher. In the case of Biomeda, I wouldn't think this is the case however I'd contact your Fisher rep and have him investigate what is happening. With all the mergers/buy-outs and switch outs it's hard to keep track of who owns what that will sell to a certain distributor that will in the end finally get you the product you requested before you've retired or gone on to the great reward. I'm a firm believer in making the sales rep earn 'their keep' as they need your business, and if your rep isn't helping go to their boss. On 1/16/08, Snider, Vivian Deanna wrote: > I have been having some issues with Biomeda also. It has been almost a > month and I still have not received the product, ordered through Fisher. > I am told it is a manufacturer's backorder. I am currently researching > a secondary source for the product. > > > > Deanna Snider HT ASCP > > Lead Histotechnician > > Shriners' Hospital for Children > > Research Dept. > > Cincinnati, OH > > > > > > CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients. If you are not the intended recipient, (or authorized to receive for the recipient) you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please destroy all copies of this communication and any attachments and contact the sender by reply e-mail or telephone (813) 281-0300. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From MLashus <@t> pathgroup.com Thu Jan 17 09:52:03 2008 From: MLashus <@t> pathgroup.com (Mighnon Lashus) Date: Thu Jan 17 09:52:30 2008 Subject: [Histonet] AE1/AE3 cross reactivity In-Reply-To: Message-ID: <197CD0B02A81F94994A285C59C8AE05C023B419A2B@pgnexchange.pathgroup.com> We do not use AR for the AE1/AE3 on our Ventana system. We use Protease 1 for 4 minutes. I will check into the heat. Any other suggestions? We do the stains for Dr. Hessler. Thanks, Mighnon Lashus -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sally Price Sent: Wednesday, January 16, 2008 5:53 PM To: Richard Hessler, M.D.; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] AE1/AE3 cross reactivity Dr. Hessler: I have observed the phenomenon that you've described for other antibodies more times than I can remember. Personally, I believe that it has to do with the way the Ventana system sprays on the retrieval solution and heats slides during antibody/detection incubation periods, which is too aggressive. I think that an open system that doesn't heat slides or perform AR isn't likely to produce these unexpalined reactions, but I digress. So, how do you fix the problem? I say disable the on-line AR, and perform it in a decloaking chamber, set at 100 degrees for 10 minutes. Or, you could consider using another pan-CK antibody, like Lu-5. Good Luck, Sally Price ---------------------------------------------------------------------------------------------- Date: Wed, 16 Jan 2008 07:57:41 -0600 From: "Richard Hessler, M.D." Subject: [Histonet] AE1/AE3 cross reactivity To: "histonet@lists.utsouthwestern.edu" " I have used this antibody for years but now have issues. Previously it was the best choice for breast sentinel nodes, but now AE1/AE3 stains plasma cells like CD138. It also stains a number of different sarcomas and beautifully highlights reactive astrocytes. I had previously used Dako Ab and autostainer with no problems. Now I have Ventanna and have noticed this with both Ventana and Dako Abs used on that system. Any thoughts??? Richard B Hessler, MD Chief of Pathology Erlanger Medical Center Chattanooga, TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you From sheila_adey <@t> hotmail.com Thu Jan 17 10:08:41 2008 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Thu Jan 17 10:09:07 2008 Subject: [Histonet] AE1/AE3 cross reactivity In-Reply-To: <197CD0B02A81F94994A285C59C8AE05C023B419A2B@pgnexchange.pathgroup.com> References: <197CD0B02A81F94994A285C59C8AE05C023B419A2B@pgnexchange.pathgroup.com> Message-ID: Our Dr.s have stopped ordering the AE1/AE3 ab aswell. For the same complaints. We use the Biogenex abs. Sheila Adey HT MLTPort Huron HospitalMichigan> From: MLashus@pathgroup.com> To: sprice2003@gmail.com; histonet@lists.utsouthwestern.edu> Date: Thu, 17 Jan 2008 09:52:03 -0600> Subject: RE: [Histonet] AE1/AE3 cross reactivity> CC: > > We do not use AR for the AE1/AE3 on our Ventana system. We use Protease 1 for 4 minutes. I will check into the heat. Any other suggestions? We do the stains for Dr. Hessler.> Thanks,> Mighnon Lashus> > -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sally Price> Sent: Wednesday, January 16, 2008 5:53 PM> To: Richard Hessler, M.D.; histonet@lists.utsouthwestern.edu> Subject: RE: [Histonet] AE1/AE3 cross reactivity> > Dr. Hessler:> I have observed the phenomenon that you've described for other antibodies> more times than I can remember. Personally, I believe that it has to do> with the way the Ventana system sprays on the retrieval solution> and heats slides during antibody/detection incubation periods, which is too> aggressive. I think that an open system that doesn't heat slides or perform> AR isn't likely to produce these unexpalined reactions, but I digress. So,> how do you fix the problem? I say disable the on-line AR, and perform it in> a decloaking chamber, set at 100 degrees for 10 minutes. Or, you could> consider using another pan-CK antibody, like Lu-5.> Good Luck,> Sally Price> > ----------------------------------------------------------------------------------------------> > Date: Wed, 16 Jan 2008 07:57:41 -0600> From: "Richard Hessler, M.D." > Subject: [Histonet] AE1/AE3 cross reactivity> To: "histonet@lists.utsouthwestern.edu"> > "> I have used this antibody for years but now have issues. Previously it was> the best choice for breast sentinel nodes, but now AE1/AE3 stains plasma> cells like CD138. It also stains a number of different sarcomas and> beautifully highlights reactive astrocytes. I had previously used Dako Ab> and autostainer with no problems. Now I have Ventanna and have noticed this> with both Ventana and Dako Abs used on that system.> > Any thoughts???> > Richard B Hessler, MD> Chief of Pathology> Erlanger Medical Center> Chattanooga, TN> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you> > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ From gregor <@t> arlt-digital.de Thu Jan 17 10:08:57 2008 From: gregor <@t> arlt-digital.de (gregor@arlt-digital.de) Date: Thu Jan 17 10:09:19 2008 Subject: [Histonet] fixation and processing whole human eyes Message-ID: <29833023.1333611200586137550.JavaMail.servlet@kundenserver> Hi All, ? I?m looking for a protocol to process whole human eyes in a vacuum infiltration system. Could anybody help with this? ?Thanks a lot. From slappycraw <@t> yahoo.com Thu Jan 17 11:43:32 2008 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Thu Jan 17 11:43:55 2008 Subject: [Histonet] Looking for chondrocyte Ab Message-ID: <625394.77121.qm@web53604.mail.re2.yahoo.com> That will bind with chondrocytes in formalin fixed paraffin embedded mouse joint tissues. Any info would be appreciated. Thanks. Larry A. Woody Seattle, Wa. ____________________________________________________________________________________ Never miss a thing. Make Yahoo your home page. http://www.yahoo.com/r/hs From mcauliff <@t> umdnj.edu Thu Jan 17 12:03:34 2008 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Jan 17 12:04:02 2008 Subject: [Histonet] Looking for chondrocyte Ab In-Reply-To: <625394.77121.qm@web53604.mail.re2.yahoo.com> References: <625394.77121.qm@web53604.mail.re2.yahoo.com> Message-ID: <478F9876.3010502@umdnj.edu> Hi Larry: Contact Vector Labs in Bulingame, CA. They may have a lectin that will do what you want. Geoff Larry Woody wrote: > That will bind with chondrocytes in formalin fixed paraffin embedded mouse joint tissues. Any info would be appreciated. Thanks. > > Larry A. Woody > Seattle, Wa. > > > ____________________________________________________________________________________ > Never miss a thing. Make Yahoo your home page. > http://www.yahoo.com/r/hs > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From Kim.Grice <@t> claripath.com Thu Jan 17 12:11:35 2008 From: Kim.Grice <@t> claripath.com (Kim Grice) Date: Thu Jan 17 12:12:29 2008 Subject: [Histonet] Nuclear Staining on Prostate Biopsies In-Reply-To: <20080117180024.285FC1740075@mail159-sin.bigfish.com> Message-ID: <49414C3E9A04974B9430CAF50347DADC03406AB9@exchangeaus.HealthTronics.com> I screamed out loud when I saw this post. We are having the same problem! Our pathologist has been driving me crazy with this issue and will just not accept that it has nothing to do with our protocols/processing. We use the Peloris tissue processor (with alcohols and xylenes) and use a 1 hr protocol. The GI biopsies we get from a local hospital (obtained in the endoscopy suite) and the prostate biopsies (obtained in the individual physician's office) are run on the same 1 hr protocol. The GI bx's look good but we have intermittent issues with the prostate cores; they look crunchy, thick and basically disgusting under the scope. I am convinced that this problem is totally out of our hands. Would you agree, or does anyone have suggestions on how to get these to look better? Thanks! Kim Grice Histology Manager ClariPath Laboratories Augusta, GA 30912 706-721-9341 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, January 17, 2008 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 50, Issue 24 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Nuclear Staining on Prostate Biopsies (Laurie Colbert) 2. no answer at Biomeda (Snider, Vivian Deanna) 3. RE: Nuclear staining on prostate biopsies (Johnson, Teri) 4. RE: AE1/AE3 cross reactivity (Tarango, Mark) 5. RE: AE1/AE3 cross reactivity (Sally Price) 6. re: Nuclear Staining on Prostate Biopsies (djemge@aol.com) 7. Re: Nuclear Staining on Prostate Biopsies (Joe Nocito) 8. Not negative (Kemlo Rogerson) 9. bone picture (louise renton) 10. Re: Not negative (Rene J Buesa) 11. Hepsin immunohistochemistry (sotiris lakis) 12. Re: no answer at Biomeda (Victoria Baker) 13. RE: AE1/AE3 cross reactivity (Mighnon Lashus) 14. RE: AE1/AE3 cross reactivity (sheila adey) 15. fixation and processing whole human eyes (gregor@arlt-digital.de) 16. Looking for chondrocyte Ab (Larry Woody) ---------------------------------------------------------------------- Message: 1 Date: Wed, 16 Jan 2008 10:17:30 -0800 From: "Laurie Colbert" Subject: RE: [Histonet] Nuclear Staining on Prostate Biopsies To: "Laurie Elmgren" Cc: histonet@lists.utsouthwestern.edu Message-ID: <57BE698966D5C54EAE8612E8941D76830248A4B6@EXCHANGE3.huntingtonhospital.com> Content-Type: text/plain; charset="us-ascii" One of the biggest problems that we encounter is that the prostate biopsies are drying out at the office before they get put into the formalin. One office in particular places the specimens on gauze before they go into formalin. I think they obtain all of the specimens and then put them all into the bottles of formalin last. We get bad staining, crunchy, dry specimens, etc. If you are having problems with a particular office, talk to them and make sure the tissue is going into formalin immediately after it is removed from the patient. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Elmgren Sent: Wednesday, January 16, 2008 9:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nuclear Staining on Prostate Biopsies I would like to optimize the quality of our stain. We process office biopsies, a good variety of tissue types. The stain is consistently very good. There are three colors of Eosin, and good nuclear detail on most tissue types, except for the prostate cores. The nuclei are stained dark and are "flat" having no depth. I am thinking that it is not the fixation, because our Monday specimens that are held over a full day are no different than those throughout the week. Any suggestions before I start reprogramming the stainer? Laurie Elmgren Histology Supervisor Sunrise Medical Labs 240 Motor Pkwy Hauppauge, NY 11788 (631)435-1515-x1108 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 16 Jan 2008 13:17:56 -0500 From: "Snider, Vivian Deanna" Subject: [Histonet] no answer at Biomeda To: Message-ID: <84BE46B37B314D409C5A17B7BAB022D601BF9C21@IDC-EX-VS01.shriners.cc> Content-Type: text/plain; charset="us-ascii" I have been having some issues with Biomeda also. It has been almost a month and I still have not received the product, ordered through Fisher. I am told it is a manufacturer's backorder. I am currently researching a secondary source for the product. Deanna Snider HT ASCP Lead Histotechnician Shriners' Hospital for Children Research Dept. Cincinnati, OH CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients. If you are not the intended recipient, (or authorized to receive for the recipient) you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please destroy all copies of this communication and any attachments and contact the sender by reply e-mail or telephone (813) 281-0300. ------------------------------ Message: 3 Date: Wed, 16 Jan 2008 12:56:20 -0600 From: "Johnson, Teri" Subject: [Histonet] RE: Nuclear staining on prostate biopsies To: Message-ID: Content-Type: text/plain; charset="us-ascii" Laurie, contact the operating room (or doctor's office) staff and ask them how the samples are handled when they are released from the biopsy gun. My guess is they are put on dry gauze and some air drying is taking place prior to being fixed. Or they are placed in some other medium besides fixative immediately. Many times the damage is already done before we ever receive the specimen. It's just a guess on my part, but mostly I think it's because all the other tissue types look good with your processing and staining times. Good luck! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 >>Laurie wrote: I would like to optimize the quality of our stain. We process office biopsies, a good variety of tissue types. The stain is consistently very good. There are three colors of Eosin, and good nuclear detail on most tissue types, except for the prostate cores. The nuclei are stained dark and are "flat" having no depth. I am thinking that it is not the fixation, because our Monday specimens that are held over a full day are no different than those throughout the week. Any suggestions before I start reprogramming the stainer? Laurie Elmgren Histology Supervisor Sunrise Medical Labs 240 Motor Pkwy Hauppauge, NY 11788 (631)435-1515-x1108 ------------------------------ Message: 4 Date: Wed, 16 Jan 2008 12:45:14 -0800 From: "Tarango, Mark" Subject: RE: [Histonet] AE1/AE3 cross reactivity To: "Richard Hessler, M.D." , histonet@lists.utsouthwestern.edu Message-ID: <5AEC610C1CE02945BD63A395BA763EDE01E48CDE@NVCIEXCH02.NVCI.org> Content-Type: text/plain; charset=us-ascii I noticed the plasma cell staining just yesterday. We use Ventana's pancytokeratin cocktail (AE1/AE3/PCK26). I was using LU-5 for pankeratin previously, but had problems with that antibody (no staining). Mark Adam Tarango HT(ASCP)QIHC Histology & IHC Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 Direct Line (702) 822-5112 Treo (702) 759-9229 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Hessler, M.D. Sent: Wednesday, January 16, 2008 5:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AE1/AE3 cross reactivity I have used this antibody for years but now have issues. Previously it was the best choice for breast sentinel nodes, but now AE1/AE3 stains plasma cells like CD138. It also stains a number of different sarcomas and beautifully highlights reactive astrocytes. I had previously used Dako Ab and autostainer with no problems. Now I have Ventanna and have noticed this with both Ventana and Dako Abs used on that system. Any thoughts??? Richard B Hessler, MD Chief of Pathology Erlanger Medical Center Chattanooga, TN ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== ------------------------------ Message: 5 Date: Wed, 16 Jan 2008 17:52:30 -0500 From: "Sally Price" Subject: RE: [Histonet] AE1/AE3 cross reactivity To: rhessler@pathgroup.com, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Dr. Hessler: I have observed the phenomenon that you've described for other antibodies more times than I can remember. Personally, I believe that it has to do with the way the Ventana system sprays on the retrieval solution and heats slides during antibody/detection incubation periods, which is too aggressive. I think that an open system that doesn't heat slides or perform AR isn't likely to produce these unexpalined reactions, but I digress. So, how do you fix the problem? I say disable the on-line AR, and perform it in a decloaking chamber, set at 100 degrees for 10 minutes. Or, you could consider using another pan-CK antibody, like Lu-5. Good Luck, Sally Price ---------------------------------------------------------------------------------------------- Date: Wed, 16 Jan 2008 07:57:41 -0600 From: "Richard Hessler, M.D." Subject: [Histonet] AE1/AE3 cross reactivity To: "histonet@lists.utsouthwestern.edu" " I have used this antibody for years but now have issues. Previously it was the best choice for breast sentinel nodes, but now AE1/AE3 stains plasma cells like CD138. It also stains a number of different sarcomas and beautifully highlights reactive astrocytes. I had previously used Dako Ab and autostainer with no problems. Now I have Ventanna and have noticed this with both Ventana and Dako Abs used on that system. Any thoughts??? Richard B Hessler, MD Chief of Pathology Erlanger Medical Center Chattanooga, TN ------------------------------ Message: 6 Date: Wed, 16 Jan 2008 18:54:45 -0500 From: djemge@aol.com Subject: [Histonet] re: Nuclear Staining on Prostate Biopsies To: histonet@lists.utsouthwestern.edu Message-ID: <8CA26B24E3B1125-8AC-4B4@MBLK-M08.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" This dark nuclei is often seen when tissue is left to dry for a few minutes before being fixed, or?if underfixed and?the alcohols on the processor fix the tissue. Since you are sure it is not dried or underfixed then it has to be the processing schedule. I agree with Renee. This sounds like a processing problem. Sounds like the overall schedule is too long for these biopsies. Especially if too long in paraffin. Donna Donna Emge HT-ASCP Northwestern University Feinburg School of Medicine 303 E. Superior, Lurie 7-220 Chicago, IL 60611 312-503-2036 d-emge@northwestern.edu ________________________________________________________________________ More new features than ever. Check out the new AOL Mail ! - http://webmail.aol.com ------------------------------ Message: 7 Date: Wed, 16 Jan 2008 17:58:28 -0600 From: "Joe Nocito" Subject: Re: [Histonet] Nuclear Staining on Prostate Biopsies To: "Laurie Elmgren" , Message-ID: <004601c8589b$c949c7d0$0302a8c0@yourxhtr8hvc4p> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Laurie, at my last place, we had to set up a different staining program for the reasons you stated. We decreased the hematoxylin and differentiation times, increased the bluing time and increased the wash time. I set up testing as follows: Group 1: hema at 30 second intervals 2:00, 2:30, 3:00, 3:30 , everything else stayed the same (4 slides) Group 2 : hema at 30 second intervals , but increase bluing 15 second intervals 30, 45, 60, 75 seconds, everything else stayed the same (16 slides) Group 3: hema 30 second intervals, bluing 15 second intervals and differentiation time by 15 seconds (64 slides, I think) Lot of work, no doubt, but that's what the pathologists wanted. Have fun and good luck JTT ----- Original Message ----- From: "Laurie Elmgren" To: Sent: Wednesday, January 16, 2008 11:42 AM Subject: [Histonet] Nuclear Staining on Prostate Biopsies I would like to optimize the quality of our stain. We process office biopsies, a good variety of tissue types. The stain is consistently very good. There are three colors of Eosin, and good nuclear detail on most tissue types, except for the prostate cores. The nuclei are stained dark and are "flat" having no depth. I am thinking that it is not the fixation, because our Monday specimens that are held over a full day are no different than those throughout the week. Any suggestions before I start reprogramming the stainer? Laurie Elmgren Histology Supervisor Sunrise Medical Labs 240 Motor Pkwy Hauppauge, NY 11788 (631)435-1515-x1108 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Thu, 17 Jan 2008 08:51:20 -0000 From: "Kemlo Rogerson" Subject: [Histonet] Not negative To: Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F4BB@wahtntex2.waht.swest.nhs.uk> Content-Type: text/plain; charset="us-ascii" I think we had a discussion last week about Pathologists reporting negative slides; interestingly there's a television news story that just broken about a Welsh Pathologist wrongly reporting biopsies. I gather there are a number of biopsies that were initially reported as negative in which the Patients subsequently developed cancer; or that's what I think the story said. This Pathologists work over a number of years is to be reviewed; reminds me of the bad years in Cytology. Abnormal slides are peer reviewed but not 'negative' ones which is odd; if you diagnosed something then the Patient will be treated (or further investigated which might flag up errors), if you call something negative then the Patient may have no treatment unless the symptoms remain and further tests reveal the abnormality. How gold is the gold standard of tissue diagnosis? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Don't be afraid to take a big step when one is indicated. You can't cross a chasm in two small jumps. --Buckminster Fuller This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation ------------------------------ Message: 9 Date: Thu, 17 Jan 2008 13:37:20 +0200 From: "louise renton" Subject: [Histonet] bone picture To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi all, is anyone willing to share a low power view of an undecalcified MMA bone section?? I desperately need one for a talk I am giving and our camera has gone all funny and won't take low power pictures...any help at all, please????? best regards- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ------------------------------ Message: 10 Date: Thu, 17 Jan 2008 06:19:34 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Not negative To: Kemlo Rogerson , histonet@lists.utsouthwestern.edu Message-ID: <540032.28982.qm@web61221.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 This reinforces my view that a "false negative" is always worse that a "false positive" although some pathlogists disagree. With a "false negative" you do nothing and with a "false positive" you do something even if it is not necessary, you err in the "cautious side". Ren? J. Kemlo Rogerson wrote: --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. ------------------------------ Message: 11 Date: Thu, 17 Jan 2008 14:51:58 +0000 (GMT) From: sotiris lakis Subject: [Histonet] Hepsin immunohistochemistry To: histonet@lists.utsouthwestern.edu Message-ID: <455401.58701.qm@web23001.mail.ird.yahoo.com> Content-Type: text/plain; charset=iso-8859-7 Hello everybody, I am interested in performing immunohistochemistry with hepsin on paraffin sections from prostate carcinoma tissue. I would be greatful if somebody who has already tested any of the antibodies commercially availiable would give me a hint about what to expect. Thank you in advance Sotiris Lakis --------------------------------- ?????????????? Yahoo! ?????????? ?? ?????????? ???? ???? (spam); ?? Yahoo! Mail ???????? ??? ???????? ?????? ????????? ???? ??? ??????????? ????????? http://login.yahoo.com/config/mail?.intl=gr ------------------------------ Message: 12 Date: Thu, 17 Jan 2008 09:53:05 -0500 From: "Victoria Baker" Subject: Re: [Histonet] no answer at Biomeda To: "Snider, Vivian Deanna" Cc: histonet@lists.utsouthwestern.edu Message-ID: <4f016b690801170653s605768c1q77874a796f6a683f@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 I had a situation a couple of years back where I was purchasing supplies through Fisher and also experienced LONG waits for receipt of products. It turned out that in some instances Fisher has to go through another distributor as the direct manufacturer will not sell direct to Fisher. In the case of Biomeda, I wouldn't think this is the case however I'd contact your Fisher rep and have him investigate what is happening. With all the mergers/buy-outs and switch outs it's hard to keep track of who owns what that will sell to a certain distributor that will in the end finally get you the product you requested before you've retired or gone on to the great reward. I'm a firm believer in making the sales rep earn 'their keep' as they need your business, and if your rep isn't helping go to their boss. On 1/16/08, Snider, Vivian Deanna wrote: > I have been having some issues with Biomeda also. It has been almost a > month and I still have not received the product, ordered through Fisher. > I am told it is a manufacturer's backorder. I am currently researching > a secondary source for the product. > > > > Deanna Snider HT ASCP > > Lead Histotechnician > > Shriners' Hospital for Children > > Research Dept. > > Cincinnati, OH > > > > > > CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients. If you are not the intended recipient, (or authorized to receive for the recipient) you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please destroy all copies of this communication and any attachments and contact the sender by reply e-mail or telephone (813) 281-0300. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 13 Date: Thu, 17 Jan 2008 09:52:03 -0600 From: Mighnon Lashus Subject: RE: [Histonet] AE1/AE3 cross reactivity To: Sally Price , "histonet@lists.utsouthwestern.edu" Message-ID: <197CD0B02A81F94994A285C59C8AE05C023B419A2B@pgnexchange.pathgroup.com> Content-Type: text/plain; charset="us-ascii" We do not use AR for the AE1/AE3 on our Ventana system. We use Protease 1 for 4 minutes. I will check into the heat. Any other suggestions? We do the stains for Dr. Hessler. Thanks, Mighnon Lashus -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sally Price Sent: Wednesday, January 16, 2008 5:53 PM To: Richard Hessler, M.D.; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] AE1/AE3 cross reactivity Dr. Hessler: I have observed the phenomenon that you've described for other antibodies more times than I can remember. Personally, I believe that it has to do with the way the Ventana system sprays on the retrieval solution and heats slides during antibody/detection incubation periods, which is too aggressive. I think that an open system that doesn't heat slides or perform AR isn't likely to produce these unexpalined reactions, but I digress. So, how do you fix the problem? I say disable the on-line AR, and perform it in a decloaking chamber, set at 100 degrees for 10 minutes. Or, you could consider using another pan-CK antibody, like Lu-5. Good Luck, Sally Price ---------------------------------------------------------------------------------------------- Date: Wed, 16 Jan 2008 07:57:41 -0600 From: "Richard Hessler, M.D." Subject: [Histonet] AE1/AE3 cross reactivity To: "histonet@lists.utsouthwestern.edu" " I have used this antibody for years but now have issues. Previously it was the best choice for breast sentinel nodes, but now AE1/AE3 stains plasma cells like CD138. It also stains a number of different sarcomas and beautifully highlights reactive astrocytes. I had previously used Dako Ab and autostainer with no problems. Now I have Ventanna and have noticed this with both Ventana and Dako Abs used on that system. Any thoughts??? Richard B Hessler, MD Chief of Pathology Erlanger Medical Center Chattanooga, TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you ------------------------------ Message: 14 Date: Thu, 17 Jan 2008 11:08:41 -0500 From: sheila adey Subject: RE: [Histonet] AE1/AE3 cross reactivity To: Mighnon Lashus , Sally Price , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Our Dr.s have stopped ordering the AE1/AE3 ab aswell. For the same complaints. We use the Biogenex abs. Sheila Adey HT MLTPort Huron HospitalMichigan> From: MLashus@pathgroup.com> To: sprice2003@gmail.com; histonet@lists.utsouthwestern.edu> Date: Thu, 17 Jan 2008 09:52:03 -0600> Subject: RE: [Histonet] AE1/AE3 cross reactivity> CC: > > We do not use AR for the AE1/AE3 on our Ventana system. We use Protease 1 for 4 minutes. I will check into the heat. Any other suggestions? We do the stains for Dr. Hessler.> Thanks,> Mighnon Lashus> > -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sally Price> Sent: Wednesday, January 16, 2008 5:53 PM> To: Richard Hessler, M.D.; histonet@lists.utsouthwestern.edu> Subject: RE: [Histonet] AE1/AE3 cross reactivity> > Dr. Hessler:> I have observed the phenomenon that you've described for other antibodies> more times than I can remember. Personally, I believe that it has to do> with the way the Ventana system sprays on the retrieval solution> and heats slides during antibody/detection incubation periods, which is too> aggressive. I think that an open system that doesn't heat slides or perform> AR isn't likely to produce these unexpalined reactions, but I digress. So,> how do you fix the problem? I say disable the on-line AR, and perform it in> a decloaking chamber, set at 100 degrees for 10 minutes. Or, you could> consider using another pan-CK antibody, like Lu-5.> Good Luck,> Sally Price> > ----------------------------------------------------------------------------------------------> > Date: Wed, 16 Jan 2008 07:57:41 -0600> From: "Richard Hessler, M.D." > Subject: [Histonet] AE1/AE3 cross reactivity> To: "histonet@lists.utsouthwestern.edu"> > "> I have used this antibody for years but now have issues. Previously it was> the best choice for breast sentinel nodes, but now AE1/AE3 stains plasma> cells like CD138. It also stains a number of different sarcomas and> beautifully highlights reactive astrocytes. I had previously used Dako Ab> and autostainer with no problems. Now I have Ventanna and have noticed this> with both Ventana and Dako Abs used on that system.> > Any thoughts???> > Richard B Hessler, MD> Chief of Pathology> Erlanger Medical Center> Chattanooga, TN> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you> > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ ------------------------------ Message: 15 Date: Thu, 17 Jan 2008 17:08:57 +0100 From: gregor@arlt-digital.de Subject: [Histonet] fixation and processing whole human eyes To: Message-ID: <29833023.1333611200586137550.JavaMail.servlet@kundenserver> Content-Type: text/plain; charset=UTF-8 Hi All, ?? I???m looking for a protocol to process whole human eyes in a vacuum infiltration system. Could anybody help with this? ??Thanks a lot. ------------------------------ Message: 16 Date: Thu, 17 Jan 2008 09:43:32 -0800 (PST) From: Larry Woody Subject: [Histonet] Looking for chondrocyte Ab To: histonet@lists.utsouthwestern.edu Message-ID: <625394.77121.qm@web53604.mail.re2.yahoo.com> Content-Type: text/plain; charset=us-ascii That will bind with chondrocytes in formalin fixed paraffin embedded mouse joint tissues. Any info would be appreciated. Thanks. Larry A. Woody Seattle, Wa. ____________________________________________________________________________________ Never miss a thing. Make Yahoo your home page. http://www.yahoo.com/r/hs ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 50, Issue 24 **************************************** From histomjans <@t> yahoo.com Thu Jan 17 12:18:36 2008 From: histomjans <@t> yahoo.com (Melissa Jans) Date: Thu Jan 17 12:19:02 2008 Subject: [Histonet] determining fixation time for Her 2 cases Message-ID: <280120.41890.qm@web32108.mail.mud.yahoo.com> Good afternoon! I am interested in how various institutes are determining formalin fixation time on cases that go for Her 2 neu testing? Are you using your LIS or a tracking sheet? Thanks so much, I look forward to your responses... Melissa Jans University of Iowa Hospitals and Clinics --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From Maxim_71 <@t> mail.ru Thu Jan 17 12:24:11 2008 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Thu Jan 17 12:25:41 2008 Subject: [Histonet] fixation and processing whole human eyes In-Reply-To: <1200593219.1014012607@mx20.mail.ru> References: <1200593219.1014012607@mx20.mail.ru> Message-ID: <741191284.20080117212411@mail.ru> We processed the central segment of human eye manually. We have also schedule for VIP-processor, but no have processor. After formalin fixation we do dehydratation with isopropanol, then infiltration with mineral oil and paraffin. Our specimens apear OK. Maxim Peshkov Russia, Taganrog. From CIngles <@t> uwhealth.org Thu Jan 17 12:40:04 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Thu Jan 17 12:40:28 2008 Subject: [Histonet] no answer at Biomeda References: <84BE46B37B314D409C5A17B7BAB022D601BF9C21@IDC-EX-VS01.shriners.cc> <4f016b690801170653s605768c1q77874a796f6a683f@mail.gmail.com> Message-ID: <08A0A863637F1349BBFD83A96B27A50A1200CE@uwhis-xchng3.uwhis.hosp.wisc.edu> Ya, OK Don't get MEstarted on the bandwagon! Sorry in advance to those Fisher people who know how to do their jobs. But... Our stainer has been down 3 weeks so far, and am still trying to get an answer on when we will get the new part. With all the mergers, etc. the part number for a specific part has changed 3+ times. With supposedly one part number being valid in the parts and service department, but the Purchasing department computers haven't been updated yet. So the number from parts is invalid and it keeps getting kicked back out. I finally have the part ordered, but now the part is coming through another distributor and will take about "4-6" weeks to get here! I'd just order a new stainer, (ya, we have a decent budget here) but I know it would take at least that long for the stainer to get here. AAAHHHH. BTW, Does anyone have a drive motor assembly for an old Baxter GLX/Shandon Linistat GLX their not using? Will pay shipping! Sorry. The nurses are getting really crabby, and they take it out on us. THI(almost)F. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Victoria Baker Sent: Thu 1/17/2008 8:53 AM To: Snider, Vivian Deanna Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] no answer at Biomeda I had a situation a couple of years back where I was purchasing supplies through Fisher and also experienced LONG waits for receipt of products. It turned out that in some instances Fisher has to go through another distributor as the direct manufacturer will not sell direct to Fisher. In the case of Biomeda, I wouldn't think this is the case however I'd contact your Fisher rep and have him investigate what is happening. With all the mergers/buy-outs and switch outs it's hard to keep track of who owns what that will sell to a certain distributor that will in the end finally get you the product you requested before you've retired or gone on to the great reward. I'm a firm believer in making the sales rep earn 'their keep' as they need your business, and if your rep isn't helping go to their boss. From doug <@t> ppspath.com Thu Jan 17 12:44:56 2008 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Thu Jan 17 12:45:50 2008 Subject: SPAM-LOW: [Histonet] Nuclear Staining on Prostate Biopsies In-Reply-To: <49414C3E9A04974B9430CAF50347DADC03406AB9@exchangeaus.HealthTronics.com> Message-ID: Kim, I can use a "conventional" processor with a 1 hour protocol for prostate biopsies. It is to my understanding that the Peloris increases the heat to get the "rapid". I would try using an alternative processor if possible just to rule out processing. You may need to adjust your protocol on the Peloris. Another issue could be the collection or grossing of the prostate biopsies. Once these are placed on gauze or a dry paper towel the water is being removed from them. Make sure the grossing tech/PA is not doing this. It is also imperative that the biopsy is not allowed to be air dried at the time of collection. It is important to educate the physician offices. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Grice Sent: Thursday, January 17, 2008 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Nuclear Staining on Prostate Biopsies I screamed out loud when I saw this post. We are having the same problem! Our pathologist has been driving me crazy with this issue and will just not accept that it has nothing to do with our protocols/processing. We use the Peloris tissue processor (with alcohols and xylenes) and use a 1 hr protocol. The GI biopsies we get from a local hospital (obtained in the endoscopy suite) and the prostate biopsies (obtained in the individual physician's office) are run on the same 1 hr protocol. The GI bx's look good but we have intermittent issues with the prostate cores; they look crunchy, thick and basically disgusting under the scope. I am convinced that this problem is totally out of our hands. Would you agree, or does anyone have suggestions on how to get these to look better? Thanks! Kim Grice Histology Manager ClariPath Laboratories Augusta, GA 30912 706-721-9341 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, January 17, 2008 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 50, Issue 24 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Nuclear Staining on Prostate Biopsies (Laurie Colbert) 2. no answer at Biomeda (Snider, Vivian Deanna) 3. RE: Nuclear staining on prostate biopsies (Johnson, Teri) 4. RE: AE1/AE3 cross reactivity (Tarango, Mark) 5. RE: AE1/AE3 cross reactivity (Sally Price) 6. re: Nuclear Staining on Prostate Biopsies (djemge@aol.com) 7. Re: Nuclear Staining on Prostate Biopsies (Joe Nocito) 8. Not negative (Kemlo Rogerson) 9. bone picture (louise renton) 10. Re: Not negative (Rene J Buesa) 11. Hepsin immunohistochemistry (sotiris lakis) 12. Re: no answer at Biomeda (Victoria Baker) 13. RE: AE1/AE3 cross reactivity (Mighnon Lashus) 14. RE: AE1/AE3 cross reactivity (sheila adey) 15. fixation and processing whole human eyes (gregor@arlt-digital.de) 16. Looking for chondrocyte Ab (Larry Woody) ---------------------------------------------------------------------- Message: 1 Date: Wed, 16 Jan 2008 10:17:30 -0800 From: "Laurie Colbert" Subject: RE: [Histonet] Nuclear Staining on Prostate Biopsies To: "Laurie Elmgren" Cc: histonet@lists.utsouthwestern.edu Message-ID: <57BE698966D5C54EAE8612E8941D76830248A4B6@EXCHANGE3.huntingtonhospital.com> Content-Type: text/plain; charset="us-ascii" One of the biggest problems that we encounter is that the prostate biopsies are drying out at the office before they get put into the formalin. One office in particular places the specimens on gauze before they go into formalin. I think they obtain all of the specimens and then put them all into the bottles of formalin last. We get bad staining, crunchy, dry specimens, etc. If you are having problems with a particular office, talk to them and make sure the tissue is going into formalin immediately after it is removed from the patient. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Elmgren Sent: Wednesday, January 16, 2008 9:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nuclear Staining on Prostate Biopsies I would like to optimize the quality of our stain. We process office biopsies, a good variety of tissue types. The stain is consistently very good. There are three colors of Eosin, and good nuclear detail on most tissue types, except for the prostate cores. The nuclei are stained dark and are "flat" having no depth. I am thinking that it is not the fixation, because our Monday specimens that are held over a full day are no different than those throughout the week. Any suggestions before I start reprogramming the stainer? Laurie Elmgren Histology Supervisor Sunrise Medical Labs 240 Motor Pkwy Hauppauge, NY 11788 (631)435-1515-x1108 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 16 Jan 2008 13:17:56 -0500 From: "Snider, Vivian Deanna" Subject: [Histonet] no answer at Biomeda To: Message-ID: <84BE46B37B314D409C5A17B7BAB022D601BF9C21@IDC-EX-VS01.shriners.cc> Content-Type: text/plain; charset="us-ascii" I have been having some issues with Biomeda also. It has been almost a month and I still have not received the product, ordered through Fisher. I am told it is a manufacturer's backorder. I am currently researching a secondary source for the product. Deanna Snider HT ASCP Lead Histotechnician Shriners' Hospital for Children Research Dept. Cincinnati, OH CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients. If you are not the intended recipient, (or authorized to receive for the recipient) you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please destroy all copies of this communication and any attachments and contact the sender by reply e-mail or telephone (813) 281-0300. ------------------------------ Message: 3 Date: Wed, 16 Jan 2008 12:56:20 -0600 From: "Johnson, Teri" Subject: [Histonet] RE: Nuclear staining on prostate biopsies To: Message-ID: Content-Type: text/plain; charset="us-ascii" Laurie, contact the operating room (or doctor's office) staff and ask them how the samples are handled when they are released from the biopsy gun. My guess is they are put on dry gauze and some air drying is taking place prior to being fixed. Or they are placed in some other medium besides fixative immediately. Many times the damage is already done before we ever receive the specimen. It's just a guess on my part, but mostly I think it's because all the other tissue types look good with your processing and staining times. Good luck! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 >>Laurie wrote: I would like to optimize the quality of our stain. We process office biopsies, a good variety of tissue types. The stain is consistently very good. There are three colors of Eosin, and good nuclear detail on most tissue types, except for the prostate cores. The nuclei are stained dark and are "flat" having no depth. I am thinking that it is not the fixation, because our Monday specimens that are held over a full day are no different than those throughout the week. Any suggestions before I start reprogramming the stainer? Laurie Elmgren Histology Supervisor Sunrise Medical Labs 240 Motor Pkwy Hauppauge, NY 11788 (631)435-1515-x1108 ------------------------------ Message: 4 Date: Wed, 16 Jan 2008 12:45:14 -0800 From: "Tarango, Mark" Subject: RE: [Histonet] AE1/AE3 cross reactivity To: "Richard Hessler, M.D." , histonet@lists.utsouthwestern.edu Message-ID: <5AEC610C1CE02945BD63A395BA763EDE01E48CDE@NVCIEXCH02.NVCI.org> Content-Type: text/plain; charset=us-ascii I noticed the plasma cell staining just yesterday. We use Ventana's pancytokeratin cocktail (AE1/AE3/PCK26). I was using LU-5 for pankeratin previously, but had problems with that antibody (no staining). Mark Adam Tarango HT(ASCP)QIHC Histology & IHC Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 Direct Line (702) 822-5112 Treo (702) 759-9229 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Hessler, M.D. Sent: Wednesday, January 16, 2008 5:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AE1/AE3 cross reactivity I have used this antibody for years but now have issues. Previously it was the best choice for breast sentinel nodes, but now AE1/AE3 stains plasma cells like CD138. It also stains a number of different sarcomas and beautifully highlights reactive astrocytes. I had previously used Dako Ab and autostainer with no problems. Now I have Ventanna and have noticed this with both Ventana and Dako Abs used on that system. Any thoughts??? Richard B Hessler, MD Chief of Pathology Erlanger Medical Center Chattanooga, TN ________________________________ Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== ------------------------------ Message: 5 Date: Wed, 16 Jan 2008 17:52:30 -0500 From: "Sally Price" Subject: RE: [Histonet] AE1/AE3 cross reactivity To: rhessler@pathgroup.com, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Dr. Hessler: I have observed the phenomenon that you've described for other antibodies more times than I can remember. Personally, I believe that it has to do with the way the Ventana system sprays on the retrieval solution and heats slides during antibody/detection incubation periods, which is too aggressive. I think that an open system that doesn't heat slides or perform AR isn't likely to produce these unexpalined reactions, but I digress. So, how do you fix the problem? I say disable the on-line AR, and perform it in a decloaking chamber, set at 100 degrees for 10 minutes. Or, you could consider using another pan-CK antibody, like Lu-5. Good Luck, Sally Price ---------------------------------------------------------------------------------------------- Date: Wed, 16 Jan 2008 07:57:41 -0600 From: "Richard Hessler, M.D." Subject: [Histonet] AE1/AE3 cross reactivity To: "histonet@lists.utsouthwestern.edu" " I have used this antibody for years but now have issues. Previously it was the best choice for breast sentinel nodes, but now AE1/AE3 stains plasma cells like CD138. It also stains a number of different sarcomas and beautifully highlights reactive astrocytes. I had previously used Dako Ab and autostainer with no problems. Now I have Ventanna and have noticed this with both Ventana and Dako Abs used on that system. Any thoughts??? Richard B Hessler, MD Chief of Pathology Erlanger Medical Center Chattanooga, TN ------------------------------ Message: 6 Date: Wed, 16 Jan 2008 18:54:45 -0500 From: djemge@aol.com Subject: [Histonet] re: Nuclear Staining on Prostate Biopsies To: histonet@lists.utsouthwestern.edu Message-ID: <8CA26B24E3B1125-8AC-4B4@MBLK-M08.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" This dark nuclei is often seen when tissue is left to dry for a few minutes before being fixed, or?if underfixed and?the alcohols on the processor fix the tissue. Since you are sure it is not dried or underfixed then it has to be the processing schedule. I agree with Renee. This sounds like a processing problem. Sounds like the overall schedule is too long for these biopsies. Especially if too long in paraffin. Donna Donna Emge HT-ASCP Northwestern University Feinburg School of Medicine 303 E. Superior, Lurie 7-220 Chicago, IL 60611 312-503-2036 d-emge@northwestern.edu ________________________________________________________________________ More new features than ever. Check out the new AOL Mail ! - http://webmail.aol.com ------------------------------ Message: 7 Date: Wed, 16 Jan 2008 17:58:28 -0600 From: "Joe Nocito" Subject: Re: [Histonet] Nuclear Staining on Prostate Biopsies To: "Laurie Elmgren" , Message-ID: <004601c8589b$c949c7d0$0302a8c0@yourxhtr8hvc4p> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Laurie, at my last place, we had to set up a different staining program for the reasons you stated. We decreased the hematoxylin and differentiation times, increased the bluing time and increased the wash time. I set up testing as follows: Group 1: hema at 30 second intervals 2:00, 2:30, 3:00, 3:30 , everything else stayed the same (4 slides) Group 2 : hema at 30 second intervals , but increase bluing 15 second intervals 30, 45, 60, 75 seconds, everything else stayed the same (16 slides) Group 3: hema 30 second intervals, bluing 15 second intervals and differentiation time by 15 seconds (64 slides, I think) Lot of work, no doubt, but that's what the pathologists wanted. Have fun and good luck JTT ----- Original Message ----- From: "Laurie Elmgren" To: Sent: Wednesday, January 16, 2008 11:42 AM Subject: [Histonet] Nuclear Staining on Prostate Biopsies I would like to optimize the quality of our stain. We process office biopsies, a good variety of tissue types. The stain is consistently very good. There are three colors of Eosin, and good nuclear detail on most tissue types, except for the prostate cores. The nuclei are stained dark and are "flat" having no depth. I am thinking that it is not the fixation, because our Monday specimens that are held over a full day are no different than those throughout the week. Any suggestions before I start reprogramming the stainer? Laurie Elmgren Histology Supervisor Sunrise Medical Labs 240 Motor Pkwy Hauppauge, NY 11788 (631)435-1515-x1108 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Thu, 17 Jan 2008 08:51:20 -0000 From: "Kemlo Rogerson" Subject: [Histonet] Not negative To: Message-ID: <86ADE4EB583CE64799A9924684A0FBBF0222F4BB@wahtntex2.waht.swest.nhs.uk> Content-Type: text/plain; charset="us-ascii" I think we had a discussion last week about Pathologists reporting negative slides; interestingly there's a television news story that just broken about a Welsh Pathologist wrongly reporting biopsies. I gather there are a number of biopsies that were initially reported as negative in which the Patients subsequently developed cancer; or that's what I think the story said. This Pathologists work over a number of years is to be reviewed; reminds me of the bad years in Cytology. Abnormal slides are peer reviewed but not 'negative' ones which is odd; if you diagnosed something then the Patient will be treated (or further investigated which might flag up errors), if you call something negative then the Patient may have no treatment unless the symptoms remain and further tests reveal the abnormality. How gold is the gold standard of tissue diagnosis? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Don't be afraid to take a big step when one is indicated. You can't cross a chasm in two small jumps. --Buckminster Fuller This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation ------------------------------ Message: 9 Date: Thu, 17 Jan 2008 13:37:20 +0200 From: "louise renton" Subject: [Histonet] bone picture To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Hi all, is anyone willing to share a low power view of an undecalcified MMA bone section?? I desperately need one for a talk I am giving and our camera has gone all funny and won't take low power pictures...any help at all, please????? best regards- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ------------------------------ Message: 10 Date: Thu, 17 Jan 2008 06:19:34 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Not negative To: Kemlo Rogerson , histonet@lists.utsouthwestern.edu Message-ID: <540032.28982.qm@web61221.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 This reinforces my view that a "false negative" is always worse that a "false positive" although some pathlogists disagree. With a "false negative" you do nothing and with a "false positive" you do something even if it is not necessary, you err in the "cautious side". Ren? J. Kemlo Rogerson wrote: --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. ------------------------------ Message: 11 Date: Thu, 17 Jan 2008 14:51:58 +0000 (GMT) From: sotiris lakis Subject: [Histonet] Hepsin immunohistochemistry To: histonet@lists.utsouthwestern.edu Message-ID: <455401.58701.qm@web23001.mail.ird.yahoo.com> Content-Type: text/plain; charset=iso-8859-7 Hello everybody, I am interested in performing immunohistochemistry with hepsin on paraffin sections from prostate carcinoma tissue. I would be greatful if somebody who has already tested any of the antibodies commercially availiable would give me a hint about what to expect. Thank you in advance Sotiris Lakis --------------------------------- ?????????????? Yahoo! ?????????? ?? ?????????? ???? ???? (spam); ?? Yahoo! Mail ???????? ??? ???????? ?????? ????????? ???? ??? ??????????? ????????? http://login.yahoo.com/config/mail?.intl=gr ------------------------------ Message: 12 Date: Thu, 17 Jan 2008 09:53:05 -0500 From: "Victoria Baker" Subject: Re: [Histonet] no answer at Biomeda To: "Snider, Vivian Deanna" Cc: histonet@lists.utsouthwestern.edu Message-ID: <4f016b690801170653s605768c1q77874a796f6a683f@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 I had a situation a couple of years back where I was purchasing supplies through Fisher and also experienced LONG waits for receipt of products. It turned out that in some instances Fisher has to go through another distributor as the direct manufacturer will not sell direct to Fisher. In the case of Biomeda, I wouldn't think this is the case however I'd contact your Fisher rep and have him investigate what is happening. With all the mergers/buy-outs and switch outs it's hard to keep track of who owns what that will sell to a certain distributor that will in the end finally get you the product you requested before you've retired or gone on to the great reward. I'm a firm believer in making the sales rep earn 'their keep' as they need your business, and if your rep isn't helping go to their boss. On 1/16/08, Snider, Vivian Deanna wrote: > I have been having some issues with Biomeda also. It has been almost a > month and I still have not received the product, ordered through Fisher. > I am told it is a manufacturer's backorder. I am currently researching > a secondary source for the product. > > > > Deanna Snider HT ASCP > > Lead Histotechnician > > Shriners' Hospital for Children > > Research Dept. > > Cincinnati, OH > > > > > > CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients. If you are not the intended recipient, (or authorized to receive for the recipient) you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please destroy all copies of this communication and any attachments and contact the sender by reply e-mail or telephone (813) 281-0300. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 13 Date: Thu, 17 Jan 2008 09:52:03 -0600 From: Mighnon Lashus Subject: RE: [Histonet] AE1/AE3 cross reactivity To: Sally Price , "histonet@lists.utsouthwestern.edu" Message-ID: <197CD0B02A81F94994A285C59C8AE05C023B419A2B@pgnexchange.pathgroup.com> Content-Type: text/plain; charset="us-ascii" We do not use AR for the AE1/AE3 on our Ventana system. We use Protease 1 for 4 minutes. I will check into the heat. Any other suggestions? We do the stains for Dr. Hessler. Thanks, Mighnon Lashus -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sally Price Sent: Wednesday, January 16, 2008 5:53 PM To: Richard Hessler, M.D.; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] AE1/AE3 cross reactivity Dr. Hessler: I have observed the phenomenon that you've described for other antibodies more times than I can remember. Personally, I believe that it has to do with the way the Ventana system sprays on the retrieval solution and heats slides during antibody/detection incubation periods, which is too aggressive. I think that an open system that doesn't heat slides or perform AR isn't likely to produce these unexpalined reactions, but I digress. So, how do you fix the problem? I say disable the on-line AR, and perform it in a decloaking chamber, set at 100 degrees for 10 minutes. Or, you could consider using another pan-CK antibody, like Lu-5. Good Luck, Sally Price ---------------------------------------------------------------------------------------------- Date: Wed, 16 Jan 2008 07:57:41 -0600 From: "Richard Hessler, M.D." Subject: [Histonet] AE1/AE3 cross reactivity To: "histonet@lists.utsouthwestern.edu" " I have used this antibody for years but now have issues. Previously it was the best choice for breast sentinel nodes, but now AE1/AE3 stains plasma cells like CD138. It also stains a number of different sarcomas and beautifully highlights reactive astrocytes. I had previously used Dako Ab and autostainer with no problems. Now I have Ventanna and have noticed this with both Ventana and Dako Abs used on that system. Any thoughts??? Richard B Hessler, MD Chief of Pathology Erlanger Medical Center Chattanooga, TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you ------------------------------ Message: 14 Date: Thu, 17 Jan 2008 11:08:41 -0500 From: sheila adey Subject: RE: [Histonet] AE1/AE3 cross reactivity To: Mighnon Lashus , Sally Price , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Our Dr.s have stopped ordering the AE1/AE3 ab aswell. For the same complaints. We use the Biogenex abs. Sheila Adey HT MLTPort Huron HospitalMichigan> From: MLashus@pathgroup.com> To: sprice2003@gmail.com; histonet@lists.utsouthwestern.edu> Date: Thu, 17 Jan 2008 09:52:03 -0600> Subject: RE: [Histonet] AE1/AE3 cross reactivity> CC: > > We do not use AR for the AE1/AE3 on our Ventana system. We use Protease 1 for 4 minutes. I will check into the heat. Any other suggestions? We do the stains for Dr. Hessler.> Thanks,> Mighnon Lashus> > -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sally Price> Sent: Wednesday, January 16, 2008 5:53 PM> To: Richard Hessler, M.D.; histonet@lists.utsouthwestern.edu> Subject: RE: [Histonet] AE1/AE3 cross reactivity> > Dr. Hessler:> I have observed the phenomenon that you've described for other antibodies> more times than I can remember. Personally, I believe that it has to do> with the way the Ventana system sprays on the retrieval solution> and heats slides during antibody/detection incubation periods, which is too> aggressive. I think that an open system that doesn't heat slides or perform> AR isn't likely to produce these unexpalined reactions, but I digress. So,> how do you fix the problem? I say disable the on-line AR, and perform it in> a decloaking chamber, set at 100 degrees for 10 minutes. Or, you could> consider using another pan-CK antibody, like Lu-5.> Good Luck,> Sally Price> > ----------------------------------------------------------------------------------------------> > Date: Wed, 16 Jan 2008 07:57:41 -0600> From: "Richard Hessler, M.D." > Subject: [Histonet] AE1/AE3 cross reactivity> To: "histonet@lists.utsouthwestern.edu"> > "> I have used this antibody for years but now have issues. Previously it was> the best choice for breast sentinel nodes, but now AE1/AE3 stains plasma> cells like CD138. It also stains a number of different sarcomas and> beautifully highlights reactive astrocytes. I had previously used Dako Ab> and autostainer with no problems. Now I have Ventanna and have noticed this> with both Ventana and Dako Abs used on that system.> > Any thoughts???> > Richard B Hessler, MD> Chief of Pathology> Erlanger Medical Center> Chattanooga, TN> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you> > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ ------------------------------ Message: 15 Date: Thu, 17 Jan 2008 17:08:57 +0100 From: gregor@arlt-digital.de Subject: [Histonet] fixation and processing whole human eyes To: Message-ID: <29833023.1333611200586137550.JavaMail.servlet@kundenserver> Content-Type: text/plain; charset=UTF-8 Hi All, ? I???m looking for a protocol to process whole human eyes in a vacuum infiltration system. Could anybody help with this? ? Thanks a lot. ------------------------------ Message: 16 Date: Thu, 17 Jan 2008 09:43:32 -0800 (PST) From: Larry Woody Subject: [Histonet] Looking for chondrocyte Ab To: histonet@lists.utsouthwestern.edu Message-ID: <625394.77121.qm@web53604.mail.re2.yahoo.com> Content-Type: text/plain; charset=us-ascii That will bind with chondrocytes in formalin fixed paraffin embedded mouse joint tissues. Any info would be appreciated. Thanks. Larry A. Woody Seattle, Wa. ____________________________________________________________________________________ Never miss a thing. Make Yahoo your home page. http://www.yahoo.com/r/hs ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 50, Issue 24 **************************************** From laurie.colbert <@t> huntingtonhospital.com Thu Jan 17 13:14:01 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Thu Jan 17 13:14:25 2008 Subject: [Histonet] determining fixation time for Her 2 cases Message-ID: <57BE698966D5C54EAE8612E8941D76830248A67C@EXCHANGE3.huntingtonhospital.com> I figured out what time the tissue comes out of formalin on the tissue processor and distributed times to the pathologists. The pathologist or PA dictates what time the specimen went into formalin - either put into formalin by us or the time that is written on the tissue requisition by Surgery. When the pathologist dictates the micros, they figure out the total fixation time, and that time is dictated into the final report. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Melissa Jans Sent: Thursday, January 17, 2008 10:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] determining fixation time for Her 2 cases Good afternoon! I am interested in how various institutes are determining formalin fixation time on cases that go for Her 2 neu testing? Are you using your LIS or a tracking sheet? Thanks so much, I look forward to your responses... Melissa Jans University of Iowa Hospitals and Clinics --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From am3309 <@t> uga.edu Thu Jan 17 13:19:50 2008 From: am3309 <@t> uga.edu (Abigail M. Butler) Date: Thu Jan 17 13:20:13 2008 Subject: [Histonet] Histoplasma Message-ID: <20080117141950.LJQ03796@punts1.cc.uga.edu> Does anyone know of an antibody for Histoplasma to use with IHC? Help!!! Thanks Abbie Butler, HT (ASCP), QIHC University of Georgia College of Veterinary Medicine From dermpathsy <@t> gmail.com Thu Jan 17 14:01:52 2008 From: dermpathsy <@t> gmail.com (Sate Hamza) Date: Thu Jan 17 14:02:15 2008 Subject: [Histonet] CDX-2 on a Dako Immunostainer Message-ID: <8854ff80801171201m7881e1d9i73e07ec1996e567f@mail.gmail.com> Colleagues, We got the CDX-2 antibody from BioGenex and we use a DakoCytomation Autostainer. Does anybody have a protocol for this antibody? Thank you in advance. Sate -- Sate Hamza, MD, FRCPC Dermatopathologist Winnipeg, Canada From tbraud <@t> holyredeemer.com Thu Jan 17 14:03:03 2008 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Thu Jan 17 14:03:26 2008 Subject: [Histonet] RE: Gold standard of Tissue Dx In-Reply-To: Message-ID: "reminds me of the bad years in Cytology. Abnormal slides are peer reviewed but not 'negative' ones which is odd; if you diagnosed something then the Patient will be treated (or further investigated which might flag up errors), if you call something negative then the Patient may have no treatment unless the symptoms remain and further tests reveal the abnormality. How gold is the gold standard of tissue diagnosis? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311" Perhaps we will have the government step into and interfere again. I prefer to think that the answer is a documented and well defined quality assurance program where a percentage of random cases are subject to peer review "If we agree to agree, then it's arbitration. If we agree to disagree, it's QA" Terri L. Braud, HT(ASCP) Anatomic Pathology Supv. Laboratory, Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3689 --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From Shirley_PHUA <@t> hsa.gov.sg Thu Jan 17 14:01:42 2008 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Thu Jan 17 14:04:56 2008 Subject: [Histonet] Shirley Phua is out-of-office ... Message-ID: I will be out of the office from 18-01-2008 to 18-01-2008. I'll be out-of-office on 18 January 2008. Pathologists: I will process your requests when I return. If urgent, please forward your email to Henry_Kyaw@hsa.gov.sg From rfields <@t> gidocs.net Thu Jan 17 14:15:14 2008 From: rfields <@t> gidocs.net (Rosa Fields) Date: Thu Jan 17 14:15:36 2008 Subject: [Histonet] AP software? Message-ID: <2F2611250DCD6549AA3D96CE8AF1F018DAD57B@giexchange.gidocs.net> Is there an AP software out there designed by a histotech? Rosa Fields, HT (ASCP) Histology Supervisor Gastroenterology Specialties P.C. 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net From dermpathsy <@t> gmail.com Thu Jan 17 14:53:58 2008 From: dermpathsy <@t> gmail.com (Sate Hamza) Date: Thu Jan 17 14:54:24 2008 Subject: [Histonet] Re: CDX-2 on a Dako Immunostainer In-Reply-To: <8854ff80801171201m7881e1d9i73e07ec1996e567f@mail.gmail.com> References: <8854ff80801171201m7881e1d9i73e07ec1996e567f@mail.gmail.com> Message-ID: <8854ff80801171253k6e3c469ao41fa708099a7223d@mail.gmail.com> Many thanks to all for the ultra-fast and very helpful replies! .. Best regards, Sate -- Sate Hamza, MD, FRCPC Dermatopathologist Winnipeg, Canada From JMacDonald <@t> mtsac.edu Thu Jan 17 15:54:32 2008 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Jan 17 15:54:55 2008 Subject: [Histonet] California Society for Histotechnology Message-ID: For information on the CSH annual symposium please visit the CSH website at http://www.californiahistology.org/ Selects Events for more information. Vendors select Vendor Info for more information on vendor opportunities. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu From Yves.Heremans <@t> vub.ac.be Fri Jan 18 02:45:39 2008 From: Yves.Heremans <@t> vub.ac.be (Yves Heremans) Date: Fri Jan 18 02:46:08 2008 Subject: [Histonet] tissue does not sink in sucrose Message-ID: <3A180D97-7A3F-4027-8BA3-CA46514AA5D3@vub.ac.be> Dear Histonetters, I am cryoprotecting mouse pancreas tissue (after fixation with formalin) in 30% sucrose in PBS. After overnight incubation in sucrose (at 4?C) some tissues sink, others do not. Is there a reason (and remedy) for this ? Thanks in advance, Yves Heremans Brussels Free University - VUB Diabetes Research Center Building D Level 2 Room D258 Laarbeeklaan 103 1090 Brussels, Belgium Phone: +32 (0)2 477 44 73 www.betacelltherapy.org From Clare_Thornton <@t> dahlchase.com Fri Jan 18 08:15:08 2008 From: Clare_Thornton <@t> dahlchase.com (Clare Thornton) Date: Fri Jan 18 08:14:37 2008 Subject: [Histonet] Equipment for sale Message-ID: We have the following pieces of used equipment for sale: Biogenex i6000 automated stainer Thermo Shandon Excelsior tissue processor Tissue Tek VIP tissue processor (holds 2 levels) If you would like more information, please email me at clare_thornton@dahlchase.com. This equipment has been lovingly maintained, always received PM's, and is in excellent condition! Clare J. Thornton, HTL(ASCP) Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street Webber West, Suite 540 Bangor, ME 04401 (207)941-8200 clare_thornton@dahlchase.com From talulahgosh <@t> gmail.com Fri Jan 18 08:25:10 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Jan 18 08:25:31 2008 Subject: [Histonet] tissue does not sink in sucrose In-Reply-To: <3A180D97-7A3F-4027-8BA3-CA46514AA5D3@vub.ac.be> References: <3A180D97-7A3F-4027-8BA3-CA46514AA5D3@vub.ac.be> Message-ID: If your tissue is large, it might not sink in one night. Leave it for a few days. Emily On Jan 18, 2008 3:45 AM, Yves Heremans wrote: > Dear Histonetters, > > I am cryoprotecting mouse pancreas tissue (after fixation with > formalin) in 30% sucrose in PBS. After overnight incubation in > sucrose (at 4?C) some tissues sink, others do not. Is there a reason > (and remedy) for this ? > > Thanks in advance, > > Yves Heremans > > Brussels Free University - VUB > Diabetes Research Center > Building D Level 2 Room D258 > Laarbeeklaan 103 > 1090 Brussels, Belgium > Phone: +32 (0)2 477 44 73 > www.betacelltherapy.org > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- "Prosperity ripened the principle of decay...and, as soon as time or accident and removed the artificial supports, the stupendous fabric yielded to the pressure of its own weight...instead of inquiring why the Roman Empire was destroyed we should rather be surprised that it has subsisted for so long" -Edward Gibbon, Decline and Fall of the Roman Empire From talulahgosh <@t> gmail.com Fri Jan 18 08:33:49 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Jan 18 08:34:13 2008 Subject: [Histonet] biomedia black market Message-ID: I have some extra biomedia mounting media. I'll put it up on ebay for $100 a bottle. 5% discount for histonetters. Shipping and handling not included. You may also want to contact Prop Joe for some street sources. Money, it's a gas, Emily -- "Prosperity ripened the principle of decay...and, as soon as time or accident and removed the artificial supports, the stupendous fabric yielded to the pressure of its own weight...instead of inquiring why the Roman Empire was destroyed we should rather be surprised that it has subsisted for so long" -Edward Gibbon, Decline and Fall of the Roman Empire From CDuke <@t> Trianglebiomedical.com Fri Jan 18 08:49:40 2008 From: CDuke <@t> Trianglebiomedical.com (Cathy Duke) Date: Fri Jan 18 08:51:44 2008 Subject: [Histonet] biomedia black market-Vendor Response Message-ID: <600E7CD630C81542ADCA1457EB6A8F4D025355E4@tbs01.trianglebiomed.com> Hey can a vendor jump in on this Blackmarket. Triangle Biomedical Sciences carries a full line of Mounting Media. We have Toulene Based, Water Based, and Xylene based for Automated coverslippers. In stock (smiles)"that's right folks, ready to ship" In the Toulene based we even carry 16 oz bottles. Call me at 919-394-9494 ext 119. Catherine Duke-Triangle Biomedical Sciences. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Emily Sours Sent: Friday, January 18, 2008 9:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] biomedia black market I have some extra biomedia mounting media. I'll put it up on ebay for $100 a bottle. 5% discount for histonetters. Shipping and handling not included. You may also want to contact Prop Joe for some street sources. Money, it's a gas, Emily -- "Prosperity ripened the principle of decay...and, as soon as time or accident and removed the artificial supports, the stupendous fabric yielded to the pressure of its own weight...instead of inquiring why the Roman Empire was destroyed we should rather be surprised that it has subsisted for so long" -Edward Gibbon, Decline and Fall of the Roman Empire _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CDuke <@t> Trianglebiomedical.com Fri Jan 18 09:03:02 2008 From: CDuke <@t> Trianglebiomedical.com (Cathy Duke) Date: Fri Jan 18 09:05:03 2008 Subject: [Histonet] biomedia black market-Vendor Response Message-ID: <600E7CD630C81542ADCA1457EB6A8F4D02CF4469@tbs01.trianglebiomed.com> I posted the wrong telephone number 919-384-9494 ext 119. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Cathy Duke Sent: Friday, January 18, 2008 9:50 AM To: Emily Sours; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] biomedia black market-Vendor Response Hey can a vendor jump in on this Blackmarket. Triangle Biomedical Sciences carries a full line of Mounting Media. We have Toulene Based, Water Based, and Xylene based for Automated coverslippers. In stock (smiles)"that's right folks, ready to ship" In the Toulene based we even carry 16 oz bottles. Call me at 919-394-9494 ext 119. Catherine Duke-Triangle Biomedical Sciences. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Emily Sours Sent: Friday, January 18, 2008 9:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] biomedia black market I have some extra biomedia mounting media. I'll put it up on ebay for $100 a bottle. 5% discount for histonetters. Shipping and handling not included. You may also want to contact Prop Joe for some street sources. Money, it's a gas, Emily -- "Prosperity ripened the principle of decay...and, as soon as time or accident and removed the artificial supports, the stupendous fabric yielded to the pressure of its own weight...instead of inquiring why the Roman Empire was destroyed we should rather be surprised that it has subsisted for so long" -Edward Gibbon, Decline and Fall of the Roman Empire _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vazquezr <@t> ohsu.edu Fri Jan 18 09:09:51 2008 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Jan 18 09:10:41 2008 Subject: [Histonet] biomedia black market-Vendor Response Message-ID: Cathy, Sorry but this venue is not for vendors to advertise. Have a good weekend,"smiles"... Robyn >>> "Cathy Duke" 1/18/2008 6:49 AM >>> Hey can a vendor jump in on this Blackmarket. Triangle Biomedical Sciences carries a full line of Mounting Media. We have Toulene Based, Water Based, and Xylene based for Automated coverslippers. In stock (smiles)"that's right folks, ready to ship" In the Toulene based we even carry 16 oz bottles. Call me at 919-394-9494 ext 119. Catherine Duke-Triangle Biomedical Sciences. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Emily Sours Sent: Friday, January 18, 2008 9:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] biomedia black market I have some extra biomedia mounting media. I'll put it up on ebay for $100 a bottle. 5% discount for histonetters. Shipping and handling not included. You may also want to contact Prop Joe for some street sources. Money, it's a gas, Emily -- "Prosperity ripened the principle of decay...and, as soon as time or accident and removed the artificial supports, the stupendous fabric yielded to the pressure of its own weight...instead of inquiring why the Roman Empire was destroyed we should rather be surprised that it has subsisted for so long" -Edward Gibbon, Decline and Fall of the Roman Empire _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Fri Jan 18 10:01:59 2008 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Jan 18 10:02:23 2008 Subject: [Histonet] tissue does not sink in sucrose In-Reply-To: <3A180D97-7A3F-4027-8BA3-CA46514AA5D3@vub.ac.be> References: <3A180D97-7A3F-4027-8BA3-CA46514AA5D3@vub.ac.be> Message-ID: <4790CD77.8070902@umdnj.edu> Hi Yeves: You may have some fat mixed in with the pancreas and fat, with at specific gravity of less than 1.0, will not sink. Geoff Yves Heremans wrote: > Dear Histonetters, > > I am cryoprotecting mouse pancreas tissue (after fixation with > formalin) in 30% sucrose in PBS. After overnight incubation in sucrose > (at 4?C) some tissues sink, others do not. Is there a reason (and > remedy) for this ? > > Thanks in advance, > > Yves Heremans > > Brussels Free University - VUB > Diabetes Research Center > Building D Level 2 Room D258 > Laarbeeklaan 103 > 1090 Brussels, Belgium > Phone: +32 (0)2 477 44 73 > www.betacelltherapy.org > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From Rcartun <@t> harthosp.org Fri Jan 18 10:58:14 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Jan 18 10:58:48 2008 Subject: [Histonet] Histoplasma In-Reply-To: <20080117141950.LJQ03796@punts1.cc.uga.edu> References: <20080117141950.LJQ03796@punts1.cc.uga.edu> Message-ID: <47909456020000770000A3BD@gwmail4.harthosp.org> The antibody that I use is from ImmunoMycologics in Norman, OK. The antibody (goat) was not intended to be used in IHC staining; however, I have been able to get it to work. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Abigail M. Butler" 01/17/08 2:19 PM >>> Does anyone know of an antibody for Histoplasma to use with IHC? Help!!! Thanks Abbie Butler, HT (ASCP), QIHC University of Georgia College of Veterinary Medicine _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From tkngflght <@t> yahoo.com Fri Jan 18 11:20:17 2008 From: tkngflght <@t> yahoo.com (Cheryl) Date: Fri Jan 18 11:20:47 2008 Subject: [Histonet] Need information on Mohs billing In-Reply-To: References: Message-ID: <006001c859f6$65fceac0$6701a8c0@FSROGER> Hello all-- A Dermatologist friend is considering adding services to his already-working clinic. He is researching the feasability including the training to do Mohs--I've already pointed him to the Moh's college site and he's still interested. Now we need to figure out the numbers... Has anyone worked up a situation like this recently? Could anyone share with me the billing codes for Mohs--both technical and professional. Is there a cap on number of specimens? How does this work for the Derm Doc performing the procedure? Diagnosing the slides? Any input/education is welcome. Thank you! Cheryl Kerry, HT(ASCP) Full Staff 281.852.9457 Personal email: tkngflght@yahoo.com From Beatrice.Debrosse-Serra <@t> pfizer.com Fri Jan 18 11:44:51 2008 From: Beatrice.Debrosse-Serra <@t> pfizer.com (Debrosse-Serra, Beatrice) Date: Fri Jan 18 11:45:14 2008 Subject: [Histonet] Pfizer, San Diego, California, Histology Opening Message-ID: <8404DFBED5207B4B8E5EEF4332CEEA53057FBB0F@lajamrexm01.amer.pfizer.com> Hi Histonetters, We still have an opening for a histologist here at Pfizer in beautiful La Jolla, CA. Below you find the job description. If you are interested, please forward your r?sum? to me. The benefits are amazing and the work environment is excellent. If you have any questions, feel free to send me an email. Thanks, Bea Responsibilities: The ideal candidate will have a high degree of proficiency in routine histology. The successful individual will be responsible for the technical procedures utilized in the preparation of microscopic slides including tissue processing, paraffin block sectioning, and H&E staining. Computer literacy is required. Main responsibilities will be production of high quality slides from frozen or fixed tissues. This will include trimming, embedding, processing, sectioning using a microtome/cryostat, and staining with H&E or other stains. Conduct slide reviews for quality control of tissue sections. Produce a quality product in a timely manner. A microtomy sectioning rate of approximately 60 blocks/day with a <3% recut rate is expected. Experience in immunohistochemistry and in situ hybridization is desirable but not essential. Other tasks may include file maintenance; other instrument and equipment maintenance; quality control checks; and related laboratory tasks. Qualifications: The successful candidate will have a Bachelor/Master degree in biology or other life sciences, be certified as a Histotechnician (HT) or Histotechnologist (HTL) and have at least 2 years of experience in a histology setting. Experience in working in a GLP environment is desirable. Proficiency in necropsy techniques or a willingness to be trained in this area is desirable. Strong written and oral communication skills are essential as the selected individual will be required to contribute to written SOP and/or in group meetings. The ability to develop effective working relationships in cross-functional teams, strong interpersonal skills, and strong troubleshooting skills are also essential. Beatrice DeBrosse-Serra Pathology Scientist Pfizer Global Research & Development CB4, 2150 10646 Science Center Drive San Diego, CA 92121 Phone# 858-622-5986 Fax# 858-678-8290 From histo20 <@t> hotmail.com Fri Jan 18 12:07:31 2008 From: histo20 <@t> hotmail.com (Paula Wilder) Date: Fri Jan 18 12:07:55 2008 Subject: [Histonet] Full-time Histo Tech position Message-ID: We have an opening at St. Joseph Medical Center, Towson, Maryland for a full-time registered Histology Technician. Please call me at 410-337-1741 if interested. Paula Wilder St. Joseph Medical Center Towson, MD 21204 410-337-1741 _________________________________________________________________ Shed those extra pounds with MSN and The Biggest Loser!! http://biggestloser.msn.com/ From RBARNHART <@t> summithealth.org Fri Jan 18 13:05:17 2008 From: RBARNHART <@t> summithealth.org (Rebecca Barnhart) Date: Fri Jan 18 13:06:21 2008 Subject: [Histonet] Knife Message-ID: <4790B21D020000570000DA9B@carrierpigeon.SummitHealth.local> I am looking for a large knife that uses disposable blades of different size. I know there is one out there but I am unsure who makes it. Thanks Becky From bdelescavage <@t> cellnetix.com Fri Jan 18 13:54:31 2008 From: bdelescavage <@t> cellnetix.com (Beth Delescavage) Date: Fri Jan 18 13:58:34 2008 Subject: [Histonet] Knife In-Reply-To: <4790B21D020000570000DA9B@carrierpigeon.SummitHealth.local> Message-ID: We have a large and small knife holder form Saukra; you can get them form Carinal Health. See link below http://www.cardinal.com/mps/catalog/ASP/D2891-6.asp?cat=Physician&mfr=Sa kura%20Finetek Unfortunately, using this system you need both handles in order to use different size blades. Thanks~ Beth Beth Delescavage, BS, HTL(ASCP) QIHC CellNetix Labs Seattle, WA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rebecca Barnhart Sent: Friday, January 18, 2008 11:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Knife I am looking for a large knife that uses disposable blades of different size. I know there is one out there but I am unsure who makes it. Thanks Becky _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From areed46254 <@t> yahoo.com Fri Jan 18 14:32:42 2008 From: areed46254 <@t> yahoo.com (amy reed) Date: Fri Jan 18 14:33:05 2008 Subject: [Histonet] RE: Mohs information Message-ID: <651772.7974.qm@web50505.mail.re2.yahoo.com> Cheryl If you will email me directly with the questions and derm info I am sure I can help with the questions that you have. Thanks Amy Reed areed46254@yahoo.com --------------------------------- Never miss a thing. Make Yahoo your homepage. From mpence <@t> grhs.net Fri Jan 18 14:52:46 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Jan 18 14:53:10 2008 Subject: [Histonet] Knife In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C876@IS-E2K3.grhs.net> Sakura Tissue-Tek Accu-Edge offers a Autopsy knife blade series that has a handle, 100mm, 170mm and brain blade you can get from your Cardinal rep. This is all I use for grossing larger specimens and doing post. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beth Delescavage Sent: Friday, January 18, 2008 1:55 PM To: histonet@lists.utsouthwestern.edu Cc: Rebecca Barnhart Subject: RE: [Histonet] Knife We have a large and small knife holder form Saukra; you can get them form Carinal Health. See link below http://www.cardinal.com/mps/catalog/ASP/D2891-6.asp?cat=Physician&mfr=Sa kura%20Finetek Unfortunately, using this system you need both handles in order to use different size blades. Thanks~ Beth Beth Delescavage, BS, HTL(ASCP) QIHC CellNetix Labs Seattle, WA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rebecca Barnhart Sent: Friday, January 18, 2008 11:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Knife I am looking for a large knife that uses disposable blades of different size. I know there is one out there but I am unsure who makes it. Thanks Becky _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dmccaig <@t> ckha.on.ca Fri Jan 18 14:46:13 2008 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Fri Jan 18 15:45:30 2008 Subject: [Histonet] ALK 1 Control Message-ID: Does have an extra positive control block (anaplastic large cell lymphoma) for ALK 1? It would be truly appreciated. Diana McCaig Histology Lab Chatham Kent Health Alliance Chatham. Ontario N8A 5E1 519-352-6401 (6604) From rjbuesa <@t> yahoo.com Fri Jan 18 16:04:05 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 18 16:04:27 2008 Subject: [Histonet] ALK 1 Control In-Reply-To: Message-ID: <346731.70887.qm@web61216.mail.yahoo.com> Cells in the umbilical cord membrane are ALK-1 positive. That was the (abundant) control I used. Ren? J. Diana McCaig wrote: Does have an extra positive control block (anaplastic large cell lymphoma) for ALK 1? It would be truly appreciated. Diana McCaig Histology Lab Chatham Kent Health Alliance Chatham. Ontario N8A 5E1 519-352-6401 (6604) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From djemge <@t> aol.com Fri Jan 18 16:28:09 2008 From: djemge <@t> aol.com (djemge@aol.com) Date: Fri Jan 18 16:28:39 2008 Subject: [Histonet] re: Tissue not sinking in sucrose Message-ID: <8CA283889A77BB1-1624-15B6@webmail-de14.sysops.aol.com> After 4%PFA I like to cryoprotect in a sucrose gradient of 15% sucrose until the tissue sinks, then 25% to 30% sucrose until it sinks again. This gives me better results when frozen sectioning delicate tissues?like mouse brain, or embryos. I agree with Goef though, if it is fatty it will not sink. Donna Emge HT-ASCP Northwestern University Feinburg School of Medicine 303 E. Superior, Lurie 7-220 Chicago, IL 60611 312-503-2036 d-emge@northwestern.edu ________________________________________________________________________ More new features than ever. Check out the new AOL Mail ! - http://webmail.aol.com From cforster <@t> umn.edu Fri Jan 18 17:31:11 2008 From: cforster <@t> umn.edu (Colleen Forster) Date: Fri Jan 18 17:31:36 2008 Subject: [Histonet] Movats Trichrome Message-ID: <479136BF.5040506@umn.edu> Looking for Gayle Callis. Would you be willing to share your Movats Trichrome protocol and reagent list...including where you purchase them from?? Thanks in advance, Colleen Forster U of MN 612-626-1930 From kcai <@t> prosci-inc.com Fri Jan 18 19:22:17 2008 From: kcai <@t> prosci-inc.com (karen Cai) Date: Fri Jan 18 19:14:52 2008 Subject: [Histonet] IHC on Paraffin cell block Message-ID: <000001c85a39$bba1fbd0$7d01a8c0@prosci.com> Hi, I am wondering if anybody have a IHC protocol for Formalin-fixed paraffin embedded cell microarray? Most of cell lines are for leukemia and other type of cancers. Your early response will be greatly appreciated. Thanks in advance, Best, Kaya No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.5.516 / Virus Database: 269.19.6/1230 - Release Date: 1/17/2008 4:59 PM From cateatmuse <@t> gmail.com Fri Jan 18 20:54:29 2008 From: cateatmuse <@t> gmail.com (Sydney Huang) Date: Fri Jan 18 20:54:52 2008 Subject: [Histonet] Does alcohol afeects the staining results of Masson's Trichrome Message-ID: Hi all I am currently encounter a problem when doing masson's trichrome, and I hope that someone can help me. The last steps in trichrome stain which are to dehydrate and clear in xylene before mount the slide, this is where it troubles me. After all the staining before dehydrating I checked the color under microscope and all colors were great, collagen blue, cytoplasma and smooth muscle red, and nucleus black. However after the dehydrating and clearing process the redness from the cytoplasma was lost. I use 2 changes of 95% alcohol then 2 changes of 100% alcohol 1 minutes each, then 2 changes of xylene also 1 minutes each. Does the freshness of the alcohol affects the results? If so, how often should I change the alcohol? Thanks in advance sincerely yours Sydney From gu.lang <@t> gmx.at Sat Jan 19 04:37:11 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Jan 19 04:37:39 2008 Subject: AW: [Histonet] Does alcohol afeects the staining results of Masson'sTrichrome In-Reply-To: Message-ID: <001e01c85a87$4049ead0$eeeea8c0@dielangs.at> Sidney, Wash your slides after anilinblue in acidified aqua dest. for 20-30 seconds. After this go directly into 100% ethanol, with 3 changes also for 20-30 seconds, then xylol and mount. Diluted ethanol tends to differentiate the staining too much. The literature says, that washing in water and ethanol decreases the intensity. I made some tests. After 30 min in acidified water or ethanol, the colours were paler but distinct. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Sydney Huang Gesendet: Samstag, 19. J?nner 2008 03:54 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Does alcohol afeects the staining results of Masson'sTrichrome Hi all I am currently encounter a problem when doing masson's trichrome, and I hope that someone can help me. The last steps in trichrome stain which are to dehydrate and clear in xylene before mount the slide, this is where it troubles me. After all the staining before dehydrating I checked the color under microscope and all colors were great, collagen blue, cytoplasma and smooth muscle red, and nucleus black. However after the dehydrating and clearing process the redness from the cytoplasma was lost. I use 2 changes of 95% alcohol then 2 changes of 100% alcohol 1 minutes each, then 2 changes of xylene also 1 minutes each. Does the freshness of the alcohol affects the results? If so, how often should I change the alcohol? Thanks in advance sincerely yours Sydney _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From immrstambo <@t> hotmail.com Sat Jan 19 08:50:05 2008 From: immrstambo <@t> hotmail.com (Christine Tambasco) Date: Sat Jan 19 08:50:29 2008 Subject: [Histonet] Fixation and staining problems Message-ID: I have been having problems with tissue fixation and/or staining. I know one can really affect the other. I have been running the same program for 20 years. I had a Pm done on my tissue processor and completely changed all reagents. The stain is not crisp and some tissue appears raw during embedding. I process all types of tissues in the same program as I had for years. We are a small 120 bed hospital and only process one run a night up to 14o blocks. We are presently using Richard Allen 10% neutral buffered formalin v/v, grades of absolute ethyl alcohol we dilute to 70%, 80% and 95% from Pharmco, Formula 83 and paraffin type 9 from Richard Allen. We also use Richard Allen Hematoxylin 7211, and Richard Allen Eosin-y Alcoholic. Everything has been the same for at least a couple years. Am I missing something? The nuclei are pale with no chromatin.(The pathologist calls it "muddy"). And why are we getting unprocessed tissue? Especially the big (prostate,uterus) and fatty type tissue. Any ideas would be greatly appreciated. I am checking with all manufacturers on the lot numbers. So far nothing.... Thanks again. Sincerely, Christine Tambasco, HT (ASCP) St. Mary's Hospital Amsterdam, New York 518-841-7287 _________________________________________________________________ Helping your favorite cause is as easy as instant messaging.?You IM, we give. http://im.live.com/Messenger/IM/Home/?source=text_hotmail_join From rjbuesa <@t> yahoo.com Sat Jan 19 09:41:18 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Jan 19 09:41:42 2008 Subject: [Histonet] Does alcohol afeects the staining results of Masson's Trichrome In-Reply-To: Message-ID: <222057.73906.qm@web61212.mail.yahoo.com> Go directly to 100% ethanol. If your "95%" has more water than it should, some fading of the stains can occur (not in the nuclei though). Ren? J. Sydney Huang wrote: --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From gayle.callis <@t> bresnan.net Sat Jan 19 10:00:20 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Sat Jan 19 10:00:41 2008 Subject: [Histonet] Movats Trichrome References: <479136BF.5040506@umn.edu> Message-ID: <002901c85ab4$648a0880$6501a8c0@DHXTS541> Colleen, I can be found on Members Only webpage/NSH website and as of September, retired. However the Movats pentachrome, rather than trichrome, was the protocol from Sheehan and Hrapchak, Theory and Practice of Histotechnology, 2nd edition. I did this over 20 years ago, but not in recent years. I know there are modified and shorter methods than the one I used, and I did it on methyl methacrylate embedded bone, ground sections. I did the protocol from Sheehan and Hrapchak as theirs was modified for methyl methacrylate plastic embedded bone but do not have this on a computer file. If you have the textbook, it will be there. I have seen messages (in Archives) which dealt with finding reagents, in particular, Safran du Gatinas or saffron which can be purchases in a grocery store. As for where to buy some of the dyes, I would be outdated for sources since the stain has not been done for many years. We did make our staining reagents in house, but you may get lucky finding a staining kit with Poly Scientific or Newcomers Supply. Google Bryan Llewellyn's Stains File website for two Movats protocols which should work very well for you, one that uses the saffron from a grocery store. A great website filled with information. I believe that Histo Logic also has a modified Movats publication. Good luck on your staining. --- Original Message ----- From: "Colleen Forster" To: Sent: Friday, January 18, 2008 4:31 PM Subject: [Histonet] Movats Trichrome > Looking for Gayle Callis. Would you be willing to share your Movats > Trichrome protocol and reagent list...including where you purchase them > from?? > > Thanks in advance, > > Colleen Forster > U of MN > 612-626-1930 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sat Jan 19 10:00:39 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Jan 19 10:01:00 2008 Subject: [Histonet] Fixation and staining problems In-Reply-To: Message-ID: <306467.61278.qm@web61213.mail.yahoo.com> It seems that you have "covered" all the potential processing troube spots. Questions: do you have a new person doing grossing that may be preparing thicker slices than before? Have you changed the time tissues are fixed outside the processor? Have you changed the schedule between sectioning and routine staining? Ren? J. Christine Tambasco wrote: --------------------------------- Never miss a thing. Make Yahoo your homepage. From LuckG <@t> empirehealth.org Sat Jan 19 15:46:31 2008 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Sat Jan 19 15:46:59 2008 Subject: [Histonet] Egg & powdered Milk A/B blocking Message-ID: <6BB8BC4519AAB844B174FC739A679BBCCEFFC7@IRMEXCH01.irm.inhs.org> Hello all, We are attempting to implement the egg white and powdered milk avidin/biotin blocking step into our steptavidin/biotin detection system to comply with CAP. We are going to do this (these steps) in a bulk fashion instead of incorporating them into our immunostainers auto-program in order to reduce the TAT. I would appreciate feedback from anyone who is using this process (introduced by Dr. Rodney Miller from Propath in Dallas). Thanks, Greg Greg Luck, B.S., HT(ASCP) Anatomic Pathology Supervisor Deaconess Med Cntr 800 W. 5th Ave Spokane, WA 99204 Offc 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmc.org From LuckG <@t> empirehealth.org Sat Jan 19 15:55:21 2008 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Sat Jan 19 15:55:43 2008 Subject: [Histonet] Slide Clips/Holders Message-ID: <6BB8BC4519AAB844B174FC739A679BBCCEFFC8@IRMEXCH01.irm.inhs.org> Hello, We are trying locate some slide clip holders. These were originally manufactured (we think) for an old model of a Smith-Kline linear stainer. The few we have are white in color, spring loaded on the slide clip end and have an approximately 3/4 inch square paddle on the opposite end. I assume they are no longer in production but if this sounds familiar to you please dig into your dusty cabinets and boxes and see if you might have any laying around and destined for the garbage next time you clean house. Thanks, Greg From pruegg <@t> ihctech.net Sat Jan 19 17:38:22 2008 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Sat Jan 19 17:32:44 2008 Subject: [Histonet] Looking for chondrocyte Ab In-Reply-To: <478F9876.3010502@umdnj.edu> Message-ID: <200801192332.m0JNWF5Q077206@pro12.abac.com> It has been a while but when I worked on bone and cartilage development we used antibodies for cartilage from U of Iowa Hybridoma Bank, one I remember is called Link Protein and there are also Collagen's that will label cartilage and chondrocytes, I forget which ones, Collagen 4 comes to mind. Contact me next week and I will give you more detailed info if you need it. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff McAuliffe Sent: Thursday, January 17, 2008 11:04 AM To: Larry Woody Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Looking for chondrocyte Ab Hi Larry: Contact Vector Labs in Bulingame, CA. They may have a lectin that will do what you want. Geoff Larry Woody wrote: > That will bind with chondrocytes in formalin fixed paraffin embedded mouse joint tissues. Any info would be appreciated. Thanks. > > Larry A. Woody > Seattle, Wa. > > > ____________________________________________________________________________ ________ > Never miss a thing. Make Yahoo your home page. > http://www.yahoo.com/r/hs > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From immrstambo <@t> hotmail.com Sat Jan 19 19:33:59 2008 From: immrstambo <@t> hotmail.com (Christine Tambasco) Date: Sat Jan 19 19:34:25 2008 Subject: [Histonet] Fixation and staining problems In-Reply-To: <306467.61278.qm@web61213.mail.yahoo.com> References: <306467.61278.qm@web61213.mail.yahoo.com> Message-ID: Everything is the same. Sections are still cut by both Pathologist. The staining sequence was recently changed to add more alcohol at the end as we previously had problems with water in the slides. I am just really stumped. Could there be something wrong with the formalin? Leica suggested I add another formula 83 to the processor and remove the 70% alcohol. I tried that too-with the same results-muddy nuclear detail.. Date: Sat, 19 Jan 2008 08:00:39 -0800From: rjbuesa@yahoo.comSubject: Re: [Histonet] Fixation and staining problemsTo: immrstambo@hotmail.com; histonet@lists.utsouthwestern.edu It seems that you have "covered" all the potential processing troube spots. Questions: do you have a new person doing grossing that may be preparing thicker slices than before? Have you changed the time tissues are fixed outside the processor? Have you changed the schedule between sectioning and routine staining? Ren? J.Christine Tambasco wrote: Never miss a thing. Make Yahoo your homepage. _________________________________________________________________ Climb to the top of the charts!?Play the word scramble challenge with star power. http://club.live.com/star_shuffle.aspx?icid=starshuffle_wlmailtextlink_jan From lpwenk <@t> sbcglobal.net Sun Jan 20 05:45:45 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sun Jan 20 05:46:14 2008 Subject: [Histonet] Online biochem/organic chem Message-ID: <000f01c85b59$fe7ed100$0202a8c0@HPPav2> Hope someone can help me (and a potential student) out with pointing me in the direction of a community college that has an on-line distance program for Intro to Organic and Biochemistry. This is a 1 semester course found at the community colleges around here. In the fall, we interviewed and accepted a student into our hospital based program, but she had to take some chemistry courses at a community college before our program started in May 2008 (had the rest of the biology/med term/math courses with the associate degree, but was missing our chemistry requirements, and maybe a physics class). Anyway, the student has taken the other classes in the fall, and was to take the intro organic/biochem class this winter. 12 hours into registration, and the class is full! She can't get in - it's full with a long waiting list. The other community college in the area only offers it the class during the day, when she works. She's supposed to start my HT program in May! I don't need her to take the lab work - the other bio and chem classes have had the students using flasks, beakers, pipets, etc. I need the student to know the theory and terminology organic chemistry (benzene, alcohol, aldehydes, etc.) and biochemistry (proteins, lipids, DNA, etc.). I'm willing to accept completion of an online class bio/organic chem class, but I can't find any via Google. Can someone get me started? Thanks from both of us. Peggy A. Wenk, HTL(ASCP)SLS Schools of Histotechnology William Beaumont Hospital Royal Oak, MI 48073 From Shawnster73 <@t> aol.com Sun Jan 20 07:44:29 2008 From: Shawnster73 <@t> aol.com (Shawnster73@aol.com) Date: Sun Jan 20 07:44:56 2008 Subject: [Histonet] Online biochem/organic chem Message-ID: There is a 5 credit online organic biochemistry class at Weber State University that includes an online lab. I have taken it, and it is a pretty good class. Someone did the labs and took pictures of each step and you do all of your lab work based off of the pictures. Hopefully they are still offering it. Michelle HT(ASCP) Boston, MA In a message dated 1/20/2008 6:46:46 A.M. Eastern Standard Time, lpwenk@sbcglobal.net writes: Hope someone can help me (and a potential student) out with pointing me in the direction of a community college that has an on-line distance program for Intro to Organic and Biochemistry. This is a 1 semester course found at the community colleges around here. In the fall, we interviewed and accepted a student into our hospital based program, but she had to take some chemistry courses at a community college before our program started in May 2008 (had the rest of the biology/med term/math courses with the associate degree, but was missing our chemistry requirements, and maybe a physics class). Anyway, the student has taken the other classes in the fall, and was to take the intro organic/biochem class this winter. 12 hours into registration, and the class is full! She can't get in - it's full with a long waiting list. The other community college in the area only offers it the class during the day, when she works. She's supposed to start my HT program in May! I don't need her to take the lab work - the other bio and chem classes have had the students using flasks, beakers, pipets, etc. I need the student to know the theory and terminology organic chemistry (benzene, alcohol, aldehydes, etc.) and biochemistry (proteins, lipids, DNA, etc.). I'm willing to accept completion of an online class bio/organic chem class, but I can't find any via Google. Can someone get me started? Thanks from both of us. Peggy A. Wenk, HTL(ASCP)SLS Schools of Histotechnology William Beaumont Hospital Royal Oak, MI 48073 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************Start the year off right. Easy ways to stay in shape. http://body.aol.com/fitness/winter-exercise?NCID=aolcmp00300000002489 From rjbuesa <@t> yahoo.com Sun Jan 20 09:30:20 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Jan 20 09:30:43 2008 Subject: [Histonet] Egg & powdered Milk A/B blocking In-Reply-To: <6BB8BC4519AAB844B174FC739A679BBCCEFFC7@IRMEXCH01.irm.inhs.org> Message-ID: <181924.17273.qm@web61215.mail.yahoo.com> Just a word of caution: IF you do not duplicate the original method, or if you do it outside the IHC sequence/protocol, you could be told that it does not comply with what is required because you are doing it "differently". Ren? J. "Luck, Greg D." wrote: --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From AnthonyH <@t> chw.edu.au Sun Jan 20 16:30:47 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Jan 20 16:31:26 2008 Subject: [Histonet] Fw: antibody validation Message-ID: Warren, I have been on holidays so am only now catching up. Did you get any responses? Here is our procedure (taken from our Manual): Scientific Approach to the Incorporation of a New Antiserum 1. Undertake a literature search to determine its clinical usefulness, drawbacks, technical problems etc. 2. Analyse the antiserum's specification sheet. 3. Perform an `Optimum Titre' on the chosen positive control tissue. 4. Use the antisera (diluted optimally) to test tissues (as many as possible) known to harbour the antigen under examination. In the case of tumours, also include examples of poor to well differentiated tumours. This will give an idea of sensitivity. 5. Test the antisera on a range of normal tissues to test for cross-reactions. Determine the significance of any positives. Test tumours arising from the normal cells. Further optimum titering may solve this problem. 6. Test the antisera on conditions/tumours that are usually entertained in the differential diagnosis. 7. Steps 5 and 6 will provide evidence for the antiserums specificity. 8. Choose a positive control that exhibits the weaker staining (will detect loss of technique sensitivity). 9. Summarise the above information and include it in this manual. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Warren_Eddings@ssmhc.com Sent: Friday, 11 January 2008 10:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fw: antibody validation -----Forwarded by Warren Eddings/=AHOKC/SSMHC on 01/10/2008 05:14PM ----- To: histonet@lists.utsouthwestern.edu From: Warren Eddings/SAHOKC/SSMHC Date: 01/08/2008 05:33PM Subjec=: antibody validation hello does anyone have a procedure for the above thanks warren_eddings@ssmhc.com _________________________________________________________________ Confidentiality Noti=ce: This email message, including any attachments, is for the sole use of t=e intended recipient(s) and may contain confidential and privileged inform=ation. Any unauthorized review, use, disclosure or distribution is prohib ited. If you are not the intended recipient, please contact the sender by=reply email and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From kellerc2 <@t> uthscsa.edu Sun Jan 20 19:47:05 2008 From: kellerc2 <@t> uthscsa.edu (Keller, Charles) Date: Sun Jan 20 19:47:26 2008 Subject: [Histonet] position available at new mouse path lab in San Antonio In-Reply-To: <200801192332.m0JNWF5Q077206@pro12.abac.com> References: <478F9876.3010502@umdnj.edu> <200801192332.m0JNWF5Q077206@pro12.abac.com> Message-ID: Dear Histonetters, Our Children's Cancer Research Institute at the academic health science center in San Antonio has a mouse path lab for which we are recruiting a full time person to manage and run. Responsibilities include histology, some special stains, and immunohistochemistry. The position will allow close interactions with researchers and interesting mouse models of disease. The facility is already very well equipped and operational. Interested persons can email me directly (kellerc2@uthscsa.edu ) for more information. Thank you. Sincerely, Charles Charles Keller, MD Assistant Professor, Department of Cellular & Structural Biology Adjunct Assistant Professor, Department of Pediatrics Director, Small Animal Imaging Facility Greehey Children's Cancer Research Institute The University of Texas Health Science Center 8403 Floyd Curl Drive, Mail Code 7784 San Antonio, TX 78229-3900 210-562-9062 [office] 210-562-9014 [fax] http://gccri.uthscsa.edu or www.sarcomalab.org kellerc2@uthscsa.edu Postdoctoral Opportunities and Undergraduate & Summer Internships: http://ccri.uthscsa.edu/keller CCRI and UTHSCSA wish to promote open communication while protecting confidential and/or privileged information. If you have received this message in error, please inform the sender and delete all copies. From rosenfeldtek <@t> hotmail.com Sun Jan 20 23:09:38 2008 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Sun Jan 20 23:10:07 2008 Subject: [Histonet] Modified Movat's Pentachrome Message-ID: That's my bread and butter stain--do it once or twice a week, and I'm spoiled for other stains--it reveals a lot of information about your specimen and the results are spectacularly beautiful. I'll forward my modified protocol if anyone wants it. Jerry Ricks Research Scientist University of Washington Department of Pathology _________________________________________________________________ Need to know the score, the latest news, or you need your Hotmail?-get your "fix". http://www.msnmobilefix.com/Default.aspx From Tiffany.Price <@t> thomaswv.org Mon Jan 21 07:38:15 2008 From: Tiffany.Price <@t> thomaswv.org (Price, Tiffany) Date: Mon Jan 21 07:39:34 2008 Subject: [Histonet] histo processing tech information Message-ID: <7B5F5D9A00739F43A1819403EC7CF49103C32531@thm-mail.thomaswv.org> I am looking for job descriptions, pay scale, etc., for techs who do specimen processing for small specimens. Any information is appreciated! Thanks Tiffany Confidentiality Notice: This email message including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privilege information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy ALL copies of the message. From b-frederick <@t> northwestern.edu Mon Jan 21 08:39:57 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Jan 21 08:40:28 2008 Subject: [Histonet] Movats Trichrome In-Reply-To: <002901c85ab4$648a0880$6501a8c0@DHXTS541> Message-ID: <000001c85c3b$81f8a100$d00f7ca5@lurie.northwestern.edu> Rowley biochemical sells a kit for Movat's. you can get the whole kit or individual reagents. WE make our own ferric chloride, alcian blue and alcoholic hematoxylin then but the rest. They send the procedure aas well. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Saturday, January 19, 2008 10:00 AM To: Colleen Forster; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Movats Trichrome Colleen, I can be found on Members Only webpage/NSH website and as of September, retired. However the Movats pentachrome, rather than trichrome, was the protocol from Sheehan and Hrapchak, Theory and Practice of Histotechnology, 2nd edition. I did this over 20 years ago, but not in recent years. I know there are modified and shorter methods than the one I used, and I did it on methyl methacrylate embedded bone, ground sections. I did the protocol from Sheehan and Hrapchak as theirs was modified for methyl methacrylate plastic embedded bone but do not have this on a computer file. If you have the textbook, it will be there. I have seen messages (in Archives) which dealt with finding reagents, in particular, Safran du Gatinas or saffron which can be purchases in a grocery store. As for where to buy some of the dyes, I would be outdated for sources since the stain has not been done for many years. We did make our staining reagents in house, but you may get lucky finding a staining kit with Poly Scientific or Newcomers Supply. Google Bryan Llewellyn's Stains File website for two Movats protocols which should work very well for you, one that uses the saffron from a grocery store. A great website filled with information. I believe that Histo Logic also has a modified Movats publication. Good luck on your staining. --- Original Message ----- From: "Colleen Forster" To: Sent: Friday, January 18, 2008 4:31 PM Subject: [Histonet] Movats Trichrome > Looking for Gayle Callis. Would you be willing to share your Movats > Trichrome protocol and reagent list...including where you purchase them > from?? > > Thanks in advance, > > Colleen Forster > U of MN > 612-626-1930 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jo-ann.bader <@t> mcgill.ca Mon Jan 21 08:38:56 2008 From: jo-ann.bader <@t> mcgill.ca (Jo-Ann Bader, Ms.) Date: Mon Jan 21 08:41:55 2008 Subject: [Histonet] Re: Fixation and Staining problem Message-ID: I would be very interested in hearing what the outcome to this problem is. I am having the same problem with the mouse tissue I am working with. Especially with the mammary gland and tumors. Jo-Ann Bader Histology Facility Coordinator Molecular Oncology Group MUHC/RVH 687 Pine Ave. W - Rm. M11-53 Montreal, QC, H3A-1A1 Tel: 514-934-1934 Ext: 31780 Fax: 514-843-1479 email: jo-ann.bader@mcgill.ca From MMcCOY <@t> lakelandregional.org Mon Jan 21 09:24:05 2008 From: MMcCOY <@t> lakelandregional.org (Mary McCoy) Date: Mon Jan 21 09:22:53 2008 Subject: [Histonet] Her 2 Neu guidelines Message-ID: I apologize if this subject has already been reviewed, but I have not been subscribed to the histonet recently. How are labs that are not routinely open on week-ends, planning on handling the 6-48 time frame for fixation established for Her2 Neu testing on surgical cases recieved on Fridays? Thanks, Mary Mary McCoy HTL(ASCP) Supervisor of Pathology Services Lakeland Regional Health System St. Joseph MI 49098 (269) 982-4891 mmccoy@lakelandregional.org -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:McCoy, Mary TEL;WORK:982-4891 ORG:;Lab TEL;PREF;FAX:98-8258 EMAIL;WORK;PREF;NGW:MMcCOY@lakelandregional.org N:McCoy;Mary TITLE:Coordinator Pathology END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:McCoy, Mary TEL;WORK:982-4891 ORG:;Lab TEL;PREF;FAX:98-8258 EMAIL;WORK;PREF;NGW:MMcCOY@lakelandregional.org N:McCoy;Mary TITLE:Coordinator Pathology TEL;PREF:982-4891 END:VCARD From rgarhart <@t> system1.net Mon Jan 21 10:32:05 2008 From: rgarhart <@t> system1.net (Robert Garhart) Date: Mon Jan 21 10:31:54 2008 Subject: [Histonet] Job Openings in GA, OH, and others In-Reply-To: <000201c77d29$5ef145f0$800aa8c0@domain.local> References: <000201c77d29$5ef145f0$800aa8c0@domain.local> Message-ID: Multiple FT opportunities in various settings throughout the US. 1. - HT/HTL position in Southern GA - Need at least 4 years experience. FISH, IHC and special stains experience is a plus. 2. - AP Manager in OH - need to have experience managing a large AP department and interacting with pathologists and third parties successfully. 3. Potential opportunities with some clients who have needs in other areas of the country as well. Call to discuss what you might be interested in or to discuss these positions above. Robert Garhart Executive Recruiter System 1 Search 678-342-9029 Office rgarhart@system1.net Website: www.system1.net Please Join my Network on Linked In http://www.linkedin.com/in/robertgarhart -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan Plaza Sent: Thursday, April 12, 2007 1:38 PM To: histonet@lists.utsouthwestern.edu; ':' Subject: [Histonet] Job Opening in East Texas Full-time Histotechnologist/Histologic Technician position available at Pathology Associates of Tyler in scenic East Texas. Should be ASCP registered or registry-eligible. P.A.T. offers a highly competitive salary with an excellent benefits package. Interested applicants should forward their resume to: Pathology Associates of Tyler, 1726 South Beckham Ave., Tyler, TX 75701. Fax: (903)592-0555. Phone: (903)593-0481 or e-mail: sgibson@tylerpathology.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tim.morken <@t> thermofisher.com Mon Jan 21 10:40:58 2008 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Mon Jan 21 10:41:41 2008 Subject: [Histonet] position available at new mouse path lab in San Antonio In-Reply-To: References: <478F9876.3010502@umdnj.edu><200801192332.m0JNWF5Q077206@pro12.abac.com> Message-ID: <6BFF6D137DF6BC43B33891BA96E83B1901172865@PGHCR-EXMB-VS-1.na.fshrnet.com> Wow, mouse pathology! And I thought it was getting a bit much with the cat and dog medical field! ;) Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Keller, Charles Sent: Sunday, January 20, 2008 5:47 PM Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] position available at new mouse path lab in San Antonio Dear Histonetters, Our Children's Cancer Research Institute at the academic health science center in San Antonio has a mouse path lab for which we are recruiting a full time person to manage and run. Responsibilities include histology, some special stains, and immunohistochemistry. The position will allow close interactions with researchers and interesting mouse models of disease. The facility is already very well equipped and operational. Interested persons can email me directly (kellerc2@uthscsa.edu ) for more information. Thank you. Sincerely, Charles Charles Keller, MD Assistant Professor, Department of Cellular & Structural Biology Adjunct Assistant Professor, Department of Pediatrics Director, Small Animal Imaging Facility Greehey Children's Cancer Research Institute The University of Texas Health Science Center 8403 Floyd Curl Drive, Mail Code 7784 San Antonio, TX 78229-3900 210-562-9062 [office] 210-562-9014 [fax] http://gccri.uthscsa.edu or www.sarcomalab.org kellerc2@uthscsa.edu Postdoctoral Opportunities and Undergraduate & Summer Internships: http://ccri.uthscsa.edu/keller CCRI and UTHSCSA wish to promote open communication while protecting confidential and/or privileged information. If you have received this message in error, please inform the sender and delete all copies. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jennifer.bull <@t> northwestpathology.com Mon Jan 21 14:21:05 2008 From: jennifer.bull <@t> northwestpathology.com (Bull, Jennifer L.) Date: Mon Jan 21 14:21:28 2008 Subject: [Histonet] Whole Mount Slides Message-ID: <719B46988560834BBE7D7F72EEB7C10A018533BE@HINET1.hinet.org> I am looking for any information on Whole Mount Slides. Our pathologists would like to start doing them on Prostate and Bladder tissue. I have researched and located a microtome attachment from Leica and SurgiPath carries oversized cassettes and embedding molds. Does anyone have any information to add? Processing/cutting issues? Supplies? I would like to be as informed as possible before taking the plunge............ Thank You in advance! Jennifer Bull Histology Supervisor NW Pathology Bellingham, WA From jennifer.bull <@t> northwestpathology.com Mon Jan 21 14:40:12 2008 From: jennifer.bull <@t> northwestpathology.com (Bull, Jennifer L.) Date: Mon Jan 21 14:40:33 2008 Subject: [Histonet] on call coverage Message-ID: <719B46988560834BBE7D7F72EEB7C10A018533BF@HINET1.hinet.org> In our lab, techs receive $100.00 for carrying the pager (for a one week period) no matter what. If they are called in - they receive an additional $100.00 in addition to their regular hourly pay (Overtime if over 40 hours for the week). Hope this helps. From rjbuesa <@t> yahoo.com Mon Jan 21 14:57:31 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jan 21 14:57:54 2008 Subject: [Histonet] Whole Mount Slides In-Reply-To: <719B46988560834BBE7D7F72EEB7C10A018533BE@HINET1.hinet.org> Message-ID: <936333.43026.qm@web61221.mail.yahoo.com> The regular Minot type (rotary) microtomes usually have a maximum of 2.5 inches block travel. For larger blocks you will need a horizontal microtome, they are just what is needed for this type of specimen. Ren? J. "Bull, Jennifer L." wrote: I am looking for any information on Whole Mount Slides. Our pathologists would like to start doing them on Prostate and Bladder tissue. I have researched and located a microtome attachment from Leica and SurgiPath carries oversized cassettes and embedding molds. Does anyone have any information to add? Processing/cutting issues? Supplies? I would like to be as informed as possible before taking the plunge............ Thank You in advance! Jennifer Bull Histology Supervisor NW Pathology Bellingham, WA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From frentz <@t> acto.de Mon Jan 21 15:02:31 2008 From: frentz <@t> acto.de (Frentz@ACTO@home) Date: Mon Jan 21 15:03:00 2008 Subject: [Histonet] need your kind help preparing sections of a whole rabbit eye Message-ID: <0ML2xA-1JH3mt2H8i-0000v8@mrelayeu.kundenserver.de> Dear all, A common problem after the fixation of a rabbit's eye in 3.7% Formalin/PBS solution is to cut though the middle of the whole eye with out loosing the structural integrity, meaning ripping out the lens. I now decided to deep freeze the globe in N2 and cut it using a micro band saw, hope it will work. But the delicate part starts after that. Dehydrating the bisected tissue for paraffin embedding causes hardening of the lens. This makes the sample inappropriate for cutting causing again loss of the lens out of its holding apparatus in the paraffin block. My idea now was to embed the tissue in a water containing material like agar or gelatine. Because I do not have lots of experience doing histological preparations, I searched the web and luckily found the histonet, obviously with lots of professionals. Searching the site gave me the idea, that gelatine embedding is usually done preparing samples for the vibratome which we do not have in the lab. Do you think it can be used for slide or rotation microtoms and how thin will the slices be at best? Can you give me useful recipes and a working procedure to solve the problem because I need to observe the whole inner eyeball following impact force to the cornea from different distances? Thanks in advance!! Markus ------------------------------------------------------------------- Markus Frentz Department for Ophthalmology Research Lab Pauwelsstrasse 30 52070 Aachen Germany Fon (Lab): +49/241/80-88224 Fax (Lab): +49/241/80-3388224 web: www.augenklinik.ukaachen.de From liz <@t> premierlab.com Mon Jan 21 15:13:58 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Mon Jan 21 15:14:34 2008 Subject: [Histonet] Whole Mount Slides In-Reply-To: <669374B0F4C443A4A99101ED21B0C333@PremierLab.local> References: <719B46988560834BBE7D7F72EEB7C10A018533BE@HINET1.hinet.org> <669374B0F4C443A4A99101ED21B0C333@PremierLab.local> Message-ID: We have not cut whole mount prostate or bladder but we have sectioned whole mount inflated guinea pig lungs, and elk trachea on a semi-automated rotary microtome (Leica RM2145), but I suspect this would work on a standard microtome. The largest samples we have sectioned were the elk trachea, we used embedding L's to embed the sample and used a standard block clamp not quick release, where you screw down to tighten the block in the clamp. The largest size of block we were able to cut was 2.25 x 3 inches. We had no issues with sectioning. The guinea pig lungs fit onto 2 x 3 slides but the elk trachea barely fit on those slides (length wise). Since we were dealing with very large samples our processing took about a week. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, January 21, 2008 2:01 PM To: Bull, Jennifer L.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Whole Mount Slides The regular Minot type (rotary) microtomes usually have a maximum of 2.5 inches block travel. For larger blocks you will need a horizontal microtome, they are just what is needed for this type of specimen. Ren? J. "Bull, Jennifer L." wrote: I am looking for any information on Whole Mount Slides. Our pathologists would like to start doing them on Prostate and Bladder tissue. I have researched and located a microtome attachment from Leica and SurgiPath carries oversized cassettes and embedding molds. Does anyone have any information to add? Processing/cutting issues? Supplies? I would like to be as informed as possible before taking the plunge............ Thank You in advance! Jennifer Bull Histology Supervisor NW Pathology Bellingham, WA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Mon Jan 21 16:10:50 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Mon Jan 21 16:11:17 2008 Subject: Whole organ, eyes Re: [Histonet] Whole Mount Slides References: <936333.43026.qm@web61221.mail.yahoo.com> Message-ID: <001a01c85c7a$7b4dc940$6501a8c0@DHXTS541> If one does not have a sliding microtome, then you can rig a rotary microtome to make it work. One of the problems with large blocks is to get total clearance of the whole block face past the knife - during sectioning. You can, with a bit of ingenuity, and using a rotary microtome, a universal knife holder and a c profile square back steel knife make this type of large block sectioning possible. You need to raise the blade so it sits higher in the knife holder, and that can be achieved by using two diamond pens that are hexagonal (?) in shape - the diamond pencils we used to label plain glass slides. By stacking one diamond pencil on top of the other so flat sides from each pen touch in the middle,you place the knife on top of the upper pencil. The metal pencils are very sturdy and help prevent chatter. Then as you section, the block face clears the knife edge, so a ribbon can form. I have seen this done on an old AO 820 rotary microtome, and it should work as long as one has the universal knife holder, pens and a c profile knife. I am not sure this would work with a blade holder insert for disposable, high profile blade, but it may as long as one raises the insert in the same way. As for molds, the L shaped (embedding blocks from Electron Microscopy Sciences) are wonderful, still available, and reusable. To shape a block so it is smaller, merely melt the edges to "square up a block" using a tilted hot plate paraffin runs off into a pan). To embed, you need to have add a block holder so you can clamp the block into the microtome. You can mount wooden block squares into the back to the embedded tissue, preferably with a spacer under the wooden block above the tissue, put the hot block on top of that, and let everything cool. Hard wood is better as soft wood soaks up water if you need to soak a block. Keep some pre cut blocks hot in the embedding center, after embedding, let everything harden together. Our favortie was Electron Microscopy Sciences resin blocks - that work very well in a screw clamp holder. Once again, keep these hot until tissue and block are embedded, let all harden in place. Leica should have a block clamp for their microtomes or a used equipment dealer. We could remove these by heating but it may not be worth it. If sectioning is a really hassle, I know of a delightful tape transfer using packaging tape that is excellent for cutting eyes to maintain all structures intact on a slide. This was published by Diane Sterchi in Journal of Histotechnology approx 1988 or so. We used it for really horrible bone blocks and tried it with large eyes. It does not require Instrumedics setup, and was totally inexpensive and effective. Gayle M. Callis HT/HTL/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Rene J Buesa" To: "Bull, Jennifer L." ; Sent: Monday, January 21, 2008 1:57 PM Subject: Re: [Histonet] Whole Mount Slides > The regular Minot type (rotary) microtomes usually have a maximum of 2.5 > inches block travel. For larger blocks you will need a horizontal > microtome, they are just what is needed for this type of specimen. > Ren? J. > > "Bull, Jennifer L." wrote: > I am looking for any information on Whole Mount Slides. Our pathologists > would like to start doing them on Prostate and Bladder tissue. I have > researched and located > a microtome attachment from Leica and SurgiPath carries oversized > cassettes and embedding molds. Does anyone have any information to add? > Processing/cutting issues? Supplies? I would like to be as informed as > possible before taking the plunge............ Thank You in advance! > > Jennifer Bull > Histology Supervisor > NW Pathology > Bellingham, WA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it > now. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Jan 21 16:13:16 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jan 21 16:13:39 2008 Subject: [Histonet] need your kind help preparing sections of a whole rabbit eye In-Reply-To: <0ML2xA-1JH3mt2H8i-0000v8@mrelayeu.kundenserver.de> Message-ID: <815671.73575.qm@web61222.mail.yahoo.com> Under separate cover I am sending a procedure for whole eye miprocessing. Ren? J. "Frentz@ACTO@home" wrote: --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From cforster <@t> umn.edu Mon Jan 21 20:07:51 2008 From: cforster <@t> umn.edu (Colleen Forster) Date: Mon Jan 21 20:06:52 2008 Subject: [Histonet] CK 14 Message-ID: <47954FF7.1080008@umn.edu> To my fellow histonetters, I am going to bring CK14 on board. Can any of you please share your favorite vendors and protocols for this antibody? Thanks in advance. Colleen Forster U of MN From GauchV <@t> mail.amc.edu Tue Jan 22 06:42:15 2008 From: GauchV <@t> mail.amc.edu (Vicki Gauch) Date: Tue Jan 22 06:43:17 2008 Subject: [Histonet] Whole Mount Slides Message-ID: Jennifer, We routinely cut whole mount prostate slides. We cut them on a Leica microtome with no problem. When we embed them, we use the bottom of a Tissue Tek mega cassette on top of the wax filled metal mold - that seems to work for us. The block stays put in the chuck holder during cutting as long as you trim the sides of the mega cassette so it is paraffin free and clearance hasn't really been an issue. If you need more info, please feel free to e-mail me.... Vicki Gauch AMCH Albany. NY >>> "Bull, Jennifer L." 1/21/2008 3:21 PM >>> I am looking for any information on Whole Mount Slides. Our pathologists would like to start doing them on Prostate and Bladder tissue. I have researched and located a microtome attachment from Leica and SurgiPath carries oversized cassettes and embedding molds. Does anyone have any information to add? Processing/cutting issues? Supplies? I would like to be as informed as possible before taking the plunge............ Thank You in advance! Jennifer Bull Histology Supervisor NW Pathology Bellingham, WA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. From gagnone <@t> KGH.KARI.NET Tue Jan 22 07:50:16 2008 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Tue Jan 22 07:50:42 2008 Subject: [Histonet] Whole Mounts Message-ID: Hi Jennifer, We cut our prostate whole mounts on the Leica RM2255 automated microtome. We obtained their Super Mega Cassette Clamp, (part no 140502 38967). This clamp mounts easily onto the microtome, and holds SurgiPath SuperCassettes ( Cat No VSP59067B-BX grey in colour). The beauty of this microtome is that the stroke is 10 mm longer than our previous Reichert-Jung 2035 microtomes we used to cut these on, I believe it is now 70 mm vertically. In terms of slides, we use 75x38 up to 75x80 mm slides, with coverslips ranging from 35x50 to 48x65 mm, depending on specimen size. The slides go on our Leica automated stainer, held diagonally in the basket by other slides to keep them vertical, and coverslipped by hand. In terms of processing, we use our normal overnight processing cycle as we would for our routine surgical blocks. These sections are well-fixed before processing, cut at 4-5 mm during crossing. We have had some issues when the pathologist assistants couldn't get the sections this thin, but it hasn't happened often enough to use a longer/different processing cycle. We have been getting 4-6 slides blocks per case, and we've done 35 cases in the last year. Hope this helps, Eric Gagnon MLT Histology Laboratory Kingston General Hospital, Kingston, Ontario, Canada From MElliott <@t> mrl.ubc.ca Tue Jan 22 10:58:04 2008 From: MElliott <@t> mrl.ubc.ca (Mark Elliott) Date: Tue Jan 22 10:59:07 2008 Subject: [Histonet] NAP-2 Message-ID: <4795B01C020000D60002B346@mail.mrl.ubc.ca> Has anybody any experience with staining for NAP-2 on formalin fixed paraffin-embedded tissues? Thanks Mark ***CONFIDENTIALITY NOTICE*** This electronic message is intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From Kitty.Maxey <@t> propathlab.com Tue Jan 22 11:04:14 2008 From: Kitty.Maxey <@t> propathlab.com (Kitty Maxey) Date: Tue Jan 22 11:04:42 2008 Subject: [Histonet] Immunohistochemistry Supervisor Opening - Dallas, TX Message-ID: IMMUNOHISTOCHEMISTRY SUPERVISOR ProPath, a progressive, CAP accredited, high-volume pathology practice in Dallas, Texas is seeking an experienced Immunohistochemistry Supervisor to oversee department operations. You will be responsible for the direction and daily operation of the department, including writing and maintaining procedures, employee assignments, evaluations, equipment maintenance, supply and reagent inventory, and record management. Our desired candidate needs to have strong technical and management abilities; organizational and training skills; a minimum of 5 years of experience in histology with at least two years of supervisory experience; as well as excellent written and oral communication skills. In addition, experience with Microsoft Office and database management is required. We also require (ASCP) HT certification. IHC certification is encouraged. College degree preferred. ProPath utilizes leading technology and is a quality oriented pathology practice. Competitive salary with a sign-on bonus and relocation assistance offered. Our benefits include vacation, group medical/dental, and disability insurance, as well as a matched 401(k) plan and more. For consideration send resume to: ProPath Human Resources 8267 Elmbrook, Suite 100 Dallas, TX 75247 214/237-1775 Fax: 214/237-1825 www.propathlab.com jobs@propathlab.com EOE To learn more about ProPath, please visit http://www.ProPathLab.com ______________________________________________________________________________ This e-mail may contain confidential or privileged information. If you think you have received this e-mail in error, please advise the sender by reply e-mail and then delete this e-mail immediately. From sjchtascp <@t> yahoo.com Tue Jan 22 11:42:59 2008 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Tue Jan 22 11:43:24 2008 Subject: [Histonet] Seeking Employment Message-ID: <655256.7791.qm@web38210.mail.mud.yahoo.com> Good afternoon, I'm looking for possible employment contacts from the Rockford, Illinois area south to the Madison, Wisconsin area north. I will consider from FT to temp. Up to 3 weeks outide this area. Thanks, Steve --------------------------------- Never miss a thing. Make Yahoo your homepage. From igor.deyneko <@t> gmail.com Tue Jan 22 12:51:52 2008 From: igor.deyneko <@t> gmail.com (Igor Deyneko) Date: Tue Jan 22 12:52:15 2008 Subject: [Histonet] Decal Bone Antigen retrieval Message-ID: <35e16a770801221051u486aede8g5432ecfd1b6c34b8@mail.gmail.com> Dear histonetters! I have a question. Recently I have received decaled mouse hind paw joint slides, which I have to do IHC on. Can anyone advise an antigen retrieval solution and a method. The current method I use and has been working fine in the past is as follows: Either Citrate or DAKO Retrieval solution in a conventional pressure cooker for 20 minutes , then I would let it stand with the open lid for another 10 minutes, and finally would take the Coplin jars out and let them stand for an additional 30 mins at RT. Worked fine for tumors. Any ideas for joints??? All suggestions would be greatly appreciated. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA 02139 From liz <@t> premierlab.com Tue Jan 22 13:18:10 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Jan 22 13:18:33 2008 Subject: [Histonet] Decal Bone Antigen retrieval In-Reply-To: <2C665B4FA23F45A9B6019A8F15629C9C@PremierLab.local> References: <2C665B4FA23F45A9B6019A8F15629C9C@PremierLab.local> Message-ID: Igor Its going to depend upon what antibody you are trying to detect but if possible I would try enzyme retreival over HIER, my favorite is proteinase K, but we also use pepsin, pronase, chondroitinase, etc. It depends upon the antibody. If you have to use HIER you can its just thats it likely you are going to loose portions of the tissue. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Igor Deyneko Sent: Tuesday, January 22, 2008 12:01 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Decal Bone Antigen retrieval Dear histonetters! I have a question. Recently I have received decaled mouse hind paw joint slides, which I have to do IHC on. Can anyone advise an antigen retrieval solution and a method. The current method I use and has been working fine in the past is as follows: Either Citrate or DAKO Retrieval solution in a conventional pressure cooker for 20 minutes , then I would let it stand with the open lid for another 10 minutes, and finally would take the Coplin jars out and let them stand for an additional 30 mins at RT. Worked fine for tumors. Any ideas for joints??? All suggestions would be greatly appreciated. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA 02139 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Tue Jan 22 13:39:40 2008 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Jan 22 13:40:05 2008 Subject: [Histonet] CSH newsletter Message-ID: The next issue of the MicroTome, the newsletter for the California Society for Histotechnology is in the works. To provide our readers with up to date information we are asking for articles. If anyone is interested in contributing an article for the Microtome please contact our editor, Judi Ford at CSHNewsletter@gmail.com Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu From Ronald.Houston <@t> nationwidechildrens.org Tue Jan 22 13:45:50 2008 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Tue Jan 22 13:46:35 2008 Subject: [Histonet] CD36 and CD41 Message-ID: <979FF5962E234F45B06CF0DB7C1AABB214C84E6D@chi2k3ms01.columbuschildrens.net> Anyone aware of antibody sources for these markers that work on paraffin sections? So far I only have found ones that will work on frozens. Thanks Ronnie Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 Ronald.Houston@NationwideChildrens.org Columbus Children's Hospital is now Nationwide Children's Hospital www.NationwideChildrens.org ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From asachau <@t> titanmed.com Tue Jan 22 13:48:04 2008 From: asachau <@t> titanmed.com (April Sachau) Date: Tue Jan 22 13:51:10 2008 Subject: [Histonet] MOHS In-Reply-To: Message-ID: <7E3ACD48BA6E26408F3188FBF08693F701023E4E@titansbs1.corp.titanmed.com> Hello Histonetters! I have a 6 month (with opportunity to extend) temporary assignment available in the Midwest currently. It is a MOHS position. Great hours and great compensation! If you have experience in MOHS histology, IHC, Frozens and H+E staining or if you know someone that has the experience and is interested, please call or email me. We do offer a referral bonus of $250.00! April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com From kenneth.metzger <@t> aruplab.com Tue Jan 22 14:03:03 2008 From: kenneth.metzger <@t> aruplab.com (Metzger, Kenneth) Date: Tue Jan 22 14:01:08 2008 Subject: [Histonet] Whole Mount Prostates Message-ID: Can anyone send or refer me to a processing protocol for whole mount prostates? We haven't done them before and need some guidance. Any other secrets or tricks on the cutting and staining would be greatly appreciated. Ken Ken Metzger HTL(ASCP) Histology Supervisor ARUP Laboratories 500 Chipeta way Salt Lake City, UT 84108 801.583.2787 ext 3101 - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From gvdobbin <@t> ihis.org Tue Jan 22 14:24:05 2008 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Tue Jan 22 14:24:50 2008 Subject: [Histonet] Mucin containing cells too blue Message-ID: Hi Folks, I have been using the Surgipath Selectech reagents (H&E) for almost 3 months now and I have not had a single complaint about staining...until now. Suddenly the mucin containing cells are too blue. My first thought was to make fresh Define (the Surgipath differentiator). This did not fix the problem. Next I replaced the hematoxylin with fresh (not supposed to have to change before 2500 slides, but changed at 1500 slides to see if that would correct the problem). It has not. I was sure that fresh hematoxylin (and thererfore correct pH) would rectify the problem. What are the other usual suspects here? I should also mention our H&E's are done on the Leica Autostainer. I look forward to receiving your collective insight! Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 There is some merit in doing the right thing rather badly, but absolutely none in doing the wrong thing excellently! Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ayant pour objet une personne ou un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer compl?tement de votre syst?me informatique. From AnthonyH <@t> chw.edu.au Tue Jan 22 14:46:55 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Jan 22 14:47:38 2008 Subject: [Histonet] Mucin containing cells too blue Message-ID: Greg, Don't assume that "fresh hematoxylin (and thererfore correct pH) would rectify the problem". Incorrect pH of the Haematoxylin is the usual problem. A feature of a haematoxylin solution that has a high pH is the staining of mucin, especially in the alimentary tract. Decreasing the pH of the staining solution to about 2.5 will decrease this mucin staining. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Greg Dobbin Sent: Wednesday, 23 January 2008 7:24 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Mucin containing cells too blue Hi Folks, I have been using the Surgipath Selectech reagents (H&E) for almost 3 months now and I have not had a single complaint about staining...until now. Suddenly the mucin containing cells are too blue. My first thought was to make fresh Define (the Surgipath differentiator). This did not fix the problem. Next I replaced the hematoxylin with fresh (not supposed to have to change before 2500 slides, but changed at 1500 slides to see if that would correct the problem). It has not. I was sure that fresh hematoxylin (and thererfore correct pH) would rectify the problem. What are the other usual suspects here? I should also mention our H&E's are done on the Leica Autostainer. I look forward to receiving your collective insight! Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 There is some merit in doing the right thing rather badly, but absolutely none in doing the wrong thing excellently! Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ayant pour objet une personne ou un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer compl?tement de votre syst?me informatique. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From akemiat3377 <@t> yahoo.com Tue Jan 22 15:25:02 2008 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Tue Jan 22 15:25:26 2008 Subject: [Histonet] Mucin containing cells too blue In-Reply-To: Message-ID: <123804.90875.qm@web31314.mail.mud.yahoo.com> The pH of the Hematoxylin I developed for Biocare (CAT Hematoxylin) a progressive hematoxylin, was between pH 2.26 to 2.3. I would let it oxidize (ripened) for 3 months before releasing it for sale. It would not over-stain mucin, especially in the GI tract. --- Tony Henwood wrote: > Greg, > > Don't assume that "fresh hematoxylin (and thererfore > correct pH) would > rectify the problem". Incorrect pH of the > Haematoxylin is the usual > problem. A feature of a haematoxylin solution that > has a high pH is the > staining of mucin, especially in the alimentary > tract. Decreasing the pH > of the staining solution to about 2.5 will decrease > this mucin staining. > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, > CT(ASC) > Laboratory Manager & Senior Scientist > The Children's Hospital at Westmead, > Locked Bag 4001, Westmead, 2145, AUSTRALIA. > Tel: 612 9845 3306 > Fax: 612 9845 3318 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Greg > Dobbin > Sent: Wednesday, 23 January 2008 7:24 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Mucin containing cells too blue > > > Hi Folks, > I have been using the Surgipath Selectech reagents > (H&E) for almost 3 > months now and I have not had a single complaint > about staining...until > now. Suddenly the mucin containing cells are too > blue. My first > thought was to make fresh Define (the Surgipath > differentiator). This > did not fix the problem. Next I replaced the > hematoxylin with fresh > (not supposed to have to change before 2500 slides, > but changed at 1500 > slides to see if that would correct the problem). It > has not. > > I was sure that fresh hematoxylin (and thererfore > correct pH) would > rectify the problem. What are the other usual > suspects here? I should > also mention our H&E's are done on the Leica > Autostainer. I look forward > to receiving your collective insight! Greg > > > > Greg Dobbin, R.T. > Chief Technologist, Histology Lab > Dept. of Laboratory Medicine, > Queen Elizabeth Hospital, > P.O. Box 6600 > Charlottetown, PE C1A 8T5 > Phone: (902) 894-2337 > Fax: (902) 894-2385 > > There is some merit in doing the right thing rather > badly, > but absolutely none in doing the wrong thing > excellently! > > Statement of Confidentiality > This message (including attachments) may contain > confidential or > privileged information intended for a specific > individual or > organization. If you have received this > communication in error, please > notify the sender immediately. If you are not the > intended recipient, > you are not authorized to use, disclose, distribute, > copy, print or rely > on this email, and should promptly delete this email > from your entire > computer system. > > D?claration de confidentialit? > Le pr?sent message (y compris les annexes) peut > contenir des > renseignements confidentiels ayant pour objet une > personne ou un > organisme particulier. Si vous avez re?u la pr?sente > communication par > erreur, veuillez en informer l'exp?diteur > imm?diatement. Si vous n'?tes > pas le destinataire pr?vu, vous n'avez pas le droit > d'utiliser, > divulguer, distribuer, copier ou imprimer ce > courriel ou encore de vous > en servir, et vous devriez l'effacer compl?tement de > votre syst?me > informatique. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************* > This email and any files transmitted with it are > confidential and intended solely for the use of the > individual or entity to whom they are addressed. If > you are not the intended recipient, please delete it > and notify the sender. > > Views expressed in this message and any attachments > are those of the individual sender, and are not > necessarily the views of The Children's Hospital at > Westmead > > This note also confirms that this email message has > been > virus scanned and although no computer viruses were > detected, The Childrens Hospital at Westmead accepts > no liability for any consequential damage resulting > from email containing computer viruses. > ********************************************************************** > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Akemi Allison-Tacha, BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing Tele: (925)788-0900 E-Mail: akemiat3377@yahoo.com From gayle.callis <@t> bresnan.net Tue Jan 22 15:38:24 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Tue Jan 22 15:38:43 2008 Subject: [Histonet] Decal Bone Antigen retrieval References: <35e16a770801221051u486aede8g5432ecfd1b6c34b8@mail.gmail.com> Message-ID: <001e01c85d3f$1e055d50$6501a8c0@DHXTS541> Some people like waterbath, at just below boiling temperature. at 99C, preheat Citra buffer to working temperature while deparaffinizing sections, immerse sections for 10 min or longer, depending on protocol, remove then rinse slides with PBS, proceed with staining. This was a method given to me some years ago by an expert on retrievals. Very gentle to bone sections. You may have to optimize time for the antigen, and how you handle the section after the initial heating. Gayle M. Callis HT/HTL/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Igor Deyneko" To: Sent: Tuesday, January 22, 2008 11:51 AM Subject: [Histonet] Decal Bone Antigen retrieval > Dear histonetters! > I have a question. Recently I have received decaled mouse hind paw joint > slides, which I have to do IHC on. Can anyone advise an antigen retrieval > solution and a method. The current method I use and has been working fine > in > the past is as follows: > Either Citrate or DAKO Retrieval solution in a conventional pressure > cooker > for 20 minutes , then I would let it stand with the open lid for another > 10 > minutes, and finally would take the Coplin jars out and let them stand for > an additional 30 mins at RT. Worked fine for tumors. Any ideas for > joints??? > All suggestions would be greatly appreciated. > > Igor Deyneko > Infinity Pharmaceuticals > Cambridge, MA 02139 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Jan 22 15:55:57 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jan 22 15:56:20 2008 Subject: [Histonet] Mucin containing cells too blue In-Reply-To: Message-ID: <24388.58951.qm@web61219.mail.yahoo.com> Since sometimes it is difficult to adjust the pH of hematoxylin, make some tests by adding 10 mL of acetic / litre of hematox. If the mucin is still blue add another 10 mL until you get to the staining hues you like. Remember that hematoxylin is a pH indicator in itself. Ren? J. Greg Dobbin wrote: --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From AnthonyH <@t> chw.edu.au Tue Jan 22 16:12:17 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Jan 22 16:12:54 2008 Subject: [Histonet] Mucin containing cells too blue Message-ID: Good, I assume you would check the pH before you put it on the market. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: Akemi Allison-Tacha [mailto:akemiat3377@yahoo.com] Sent: Wednesday, 23 January 2008 8:25 AM To: Tony Henwood; Greg Dobbin; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Mucin containing cells too blue The pH of the Hematoxylin I developed for Biocare (CAT Hematoxylin) a progressive hematoxylin, was between pH 2.26 to 2.3. I would let it oxidize (ripened) for 3 months before releasing it for sale. It would not over-stain mucin, especially in the GI tract. --- Tony Henwood wrote: > Greg, > > Don't assume that "fresh hematoxylin (and thererfore > correct pH) would > rectify the problem". Incorrect pH of the > Haematoxylin is the usual > problem. A feature of a haematoxylin solution that > has a high pH is the > staining of mucin, especially in the alimentary > tract. Decreasing the pH > of the staining solution to about 2.5 will decrease > this mucin staining. > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, > CT(ASC) > Laboratory Manager & Senior Scientist > The Children's Hospital at Westmead, > Locked Bag 4001, Westmead, 2145, AUSTRALIA. > Tel: 612 9845 3306 > Fax: 612 9845 3318 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Greg > Dobbin > Sent: Wednesday, 23 January 2008 7:24 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Mucin containing cells too blue > > > Hi Folks, > I have been using the Surgipath Selectech reagents > (H&E) for almost 3 > months now and I have not had a single complaint > about staining...until > now. Suddenly the mucin containing cells are too > blue. My first > thought was to make fresh Define (the Surgipath differentiator). This > did not fix the problem. Next I replaced the > hematoxylin with fresh > (not supposed to have to change before 2500 slides, > but changed at 1500 > slides to see if that would correct the problem). It > has not. > > I was sure that fresh hematoxylin (and thererfore > correct pH) would > rectify the problem. What are the other usual > suspects here? I should > also mention our H&E's are done on the Leica > Autostainer. I look forward > to receiving your collective insight! Greg > > > > Greg Dobbin, R.T. > Chief Technologist, Histology Lab > Dept. of Laboratory Medicine, > Queen Elizabeth Hospital, > P.O. Box 6600 > Charlottetown, PE C1A 8T5 > Phone: (902) 894-2337 > Fax: (902) 894-2385 > > There is some merit in doing the right thing rather > badly, > but absolutely none in doing the wrong thing > excellently! > > Statement of Confidentiality > This message (including attachments) may contain > confidential or > privileged information intended for a specific > individual or > organization. If you have received this > communication in error, please > notify the sender immediately. If you are not the > intended recipient, > you are not authorized to use, disclose, distribute, > copy, print or rely > on this email, and should promptly delete this email > from your entire > computer system. > > D?claration de confidentialit? > Le pr?sent message (y compris les annexes) peut > contenir des > renseignements confidentiels ayant pour objet une > personne ou un > organisme particulier. Si vous avez re?u la pr?sente communication par > erreur, veuillez en informer l'exp?diteur > imm?diatement. Si vous n'?tes > pas le destinataire pr?vu, vous n'avez pas le droit > d'utiliser, > divulguer, distribuer, copier ou imprimer ce > courriel ou encore de vous > en servir, et vous devriez l'effacer compl?tement de > votre syst?me > informatique. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************* > This email and any files transmitted with it are > confidential and intended solely for the use of the individual or > entity to whom they are addressed. If you are not the intended > recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments > are those of the individual sender, and are not > necessarily the views of The Children's Hospital at > Westmead > > This note also confirms that this email message has > been > virus scanned and although no computer viruses were > detected, The Childrens Hospital at Westmead accepts > no liability for any consequential damage resulting > from email containing computer viruses. > ********************************************************************** > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Akemi Allison-Tacha, BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing Tele: (925)788-0900 E-Mail: akemiat3377@yahoo.com ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From nyilmaz <@t> mersin.edu.tr Tue Jan 22 16:20:30 2008 From: nyilmaz <@t> mersin.edu.tr (nyilmaz@mersin.edu.tr) Date: Tue Jan 22 16:21:19 2008 Subject: [Histonet] Need some cell markers for IF Message-ID: <20080122222030.B8AF833B5C6@mail.mersin.edu.tr> Dear netters, We need to prove that our cultured cells are chondrocytes exactly. We have to perform immunofluorescence, so we need antibodies spesific for chondrocytes, mesenchymal stem cells and fibroblasts. Does anybody know antibodies against spesific markers for these cells suitable for IF on cultured cells? Thanks in advance... Dr. Necat Yilmaz Mersin University School of Medicine Histology & Embryology Dept. Mersin/TURKEY ___________________________________ Mersin Universitesi, http://www.mersin.edu.tr From llewllew <@t> shaw.ca Tue Jan 22 18:19:57 2008 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Tue Jan 22 18:22:26 2008 Subject: [Histonet] Mucin containing cells too blue References: Message-ID: <001e01c85d55$af834c40$05024246@yourlk4rlmsu> If you go to their web site and look at the MSDS for the hemalum it appears to contain about 6 grams hematoxylin per litre, with both alum and aluminum sulphate for mordant, and hydrochloric acid (about 2 mL per litre). It comes 80% oxidised when fresh, if the amount of sodium iodate is anything to go by. In other words, a very strong hemalum with full oxidation and hydrochloric acid as pH control. I'm more surprised that it doesn't always stain mucin, frankly. Try adding more hydrochloric acid. It is probably the pH is too high. Mucins stain around pH3-4. Add hydrochloric acid to get it below that. Alternatively, and a MUCH better idea, use a hemalum with about 3 grams hematoxylin per litre. It is much less likely to stain mucin. To do that, make up a solvent containing everything in the hemalum except the hematoxylin and oxidant, then dilute equal parts. Or, try Coles with acid. Bryan Llewellyn ----- Original Message ----- From: "Greg Dobbin" To: Sent: Tuesday, January 22, 2008 12:24 PM Subject: [Histonet] Mucin containing cells too blue > Hi Folks, > I have been using the Surgipath Selectech reagents (H&E) for almost 3 > months now and I have not had a single complaint about staining...until > now. Suddenly the mucin containing cells are too blue. My first > thought was to make fresh Define (the Surgipath differentiator). This > did not fix the problem. Next I replaced the hematoxylin with fresh > (not supposed to have to change before 2500 slides, but changed at 1500 > slides to see if that would correct the problem). It has not. > > I was sure that fresh hematoxylin (and thererfore correct pH) would > rectify the problem. What are the other usual suspects here? I should > also mention our H&E's are done on the Leica Autostainer. > I look forward to receiving your collective insight! > Greg > > > > Greg Dobbin, R.T. > Chief Technologist, Histology Lab > Dept. of Laboratory Medicine, > Queen Elizabeth Hospital, > P.O. Box 6600 > Charlottetown, PE C1A 8T5 > Phone: (902) 894-2337 > Fax: (902) 894-2385 > > There is some merit in doing the right thing rather badly, > but absolutely none in doing the wrong thing excellently! > > Statement of Confidentiality > This message (including attachments) may contain confidential or > privileged information intended for a specific individual or organization. > If you have received this communication in error, please notify the sender > immediately. If you are not the intended recipient, you are not > authorized to use, disclose, distribute, copy, print or rely on this > email, and should promptly delete this email from your entire computer > system. > > D?claration de confidentialit? > Le pr?sent message (y compris les annexes) peut contenir des > renseignements confidentiels ayant pour objet une personne ou un organisme > particulier. Si vous avez re?u la pr?sente communication par erreur, > veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le > destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, > distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et > vous devriez l'effacer compl?tement de votre syst?me informatique. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vetdrrkc <@t> gmail.com Tue Jan 22 18:26:28 2008 From: vetdrrkc <@t> gmail.com (Ratan K) Date: Tue Jan 22 18:26:51 2008 Subject: [Histonet] shelf life of quantum dots Message-ID: Dear histonetters, I have streptovidin conjugate quantum dots, by which i am going to detect estrogen receptor. But, its manufacture date is 2004, I am interested to know that will it work now? I have not done this procedure before it. Shall I proceed with it or stop? sincerely Ratan K Choudhary -- http://www.jaxtr.com/vetdrrkc From NSEARCY <@t> swmail.sw.org Wed Jan 23 07:00:37 2008 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Wed Jan 23 07:01:09 2008 Subject: [Histonet] Mohs Tech Message-ID: Our dermatopathology department is looking for a Mohs tech. Anyone have any contacts? Thanks From rjbuesa <@t> yahoo.com Wed Jan 23 08:31:29 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 23 08:31:51 2008 Subject: [Histonet] shelf life of quantum dots In-Reply-To: Message-ID: <224406.42959.qm@web61217.mail.yahoo.com> Try to contact the manufacturer regarding an "expected" shelf life. Probably they will have a better idea. Ren? J. Ratan K wrote: Dear histonetters, I have streptovidin conjugate quantum dots, by which i am going to detect estrogen receptor. But, its manufacture date is 2004, I am interested to know that will it work now? I have not done this procedure before it. Shall I proceed with it or stop? sincerely Ratan K Choudhary -- http://www.jaxtr.com/vetdrrkc http://www.youtube.com/v/3ljEAjSY4Kk"> http://www.youtube.com/v/3ljEAjSY4Kk" type="application/x-shockwave-flash" width="425" height="350"> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From rjbuesa <@t> yahoo.com Wed Jan 23 08:32:05 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 23 08:32:30 2008 Subject: [Histonet] Mohs Tech In-Reply-To: Message-ID: <388853.84579.qm@web61221.mail.yahoo.com> Try their web site. Ren? J. Nita Searcy wrote: Our dermatopathology department is looking for a Mohs tech. Anyone have any contacts? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From jennifer.bull <@t> northwestpathology.com Wed Jan 23 09:32:48 2008 From: jennifer.bull <@t> northwestpathology.com (Bull, Jennifer L.) Date: Wed Jan 23 09:33:10 2008 Subject: [Histonet] PMS2 Antibody Message-ID: <719B46988560834BBE7D7F72EEB7C10A018533CA@HINET1.hinet.org> I am looking for PMS2 antibody (clone A16-4) as an IVD for formalin fixed parafin embedded IHC. Is anyone out there using this or know where I can find the antibody? I am also looking for MLH1, MSH2 and MSH6, but I have a couple vendors for these so far. Any input is appreciated! Jenny From gu.lang <@t> gmx.at Wed Jan 23 09:36:57 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Jan 23 09:37:23 2008 Subject: AW: [Histonet] Whole Mount Prostates In-Reply-To: Message-ID: <000101c85dd5$ca748430$eeeea8c0@dielangs.at> The prostatae are fixed in NBF at least for two days, then marked with black and green ink and sliced up in 8-10 mm slides. We work with the big cassettes and molds. The VIP-protocol for the routine specimen was just doubled in each station. The thick slides tend to bend up in the center. So embedding should be done with enough power to stick them on the ground. Our routine microtomes are sliding microtomes, so the prostatae are also cut on these and that works fine. The HE protocol is the same as with routine specimen. Hope this helps. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Metzger, Kenneth Gesendet: Dienstag, 22. J?nner 2008 21:03 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Whole Mount Prostates Can anyone send or refer me to a processing protocol for whole mount prostates? We haven't done them before and need some guidance. Any other secrets or tricks on the cutting and staining would be greatly appreciated. Ken Ken Metzger HTL(ASCP) Histology Supervisor ARUP Laboratories 500 Chipeta way Salt Lake City, UT 84108 801.583.2787 ext 3101 - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From majid.ghoddusi <@t> gmail.com Wed Jan 23 11:10:52 2008 From: majid.ghoddusi <@t> gmail.com (Majid ghoddusi) Date: Wed Jan 23 11:11:17 2008 Subject: [Histonet] Osteoblast and osteoclast staining Message-ID: Hello all, I have some paraffin-embedded Murine bone that have been decalcified with a hydrochloric acid-based decalcifying solution. I would like to stain the osteoblasts and osteoclasts in this tissue if possible. What are my options? The information that I am getting suggests that my chances of a successful staining with TRAP is somewhat limited since the tissue has been decalcified in this manner. I would appreciate any help I can get form the list. Thank you, Majid *Majid Ghoddusi, DVM, PhD* *Veterinary Pathologist* *Comparative Biosciences, Inc.* *http://www.compbio.com/* From mcauliff <@t> umdnj.edu Wed Jan 23 11:41:39 2008 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Jan 23 11:42:00 2008 Subject: [Histonet] Osteoblast and osteoclast staining In-Reply-To: References: Message-ID: <47977C53.8040308@umdnj.edu> Osteoclasts are easy to recognize in H&E staining, they are large multinucleate cells almost always found in depressions (Howship's lacuna) on the surface of bone. No other bone cell looks like an osteoclast. Osteoblasts are found on the surface of bone, any surface not just the outer surface. Osteocytes are found encased in bone matrix. Geoff Majid ghoddusi wrote: > Hello all, > > I have some paraffin-embedded Murine bone that have been decalcified with a > hydrochloric acid-based decalcifying solution. I would like to stain the > osteoblasts and osteoclasts in this tissue if possible. What are my options? > The information that I am getting suggests that my chances of a successful > staining with TRAP is somewhat limited since the tissue has been decalcified > in this manner. I would appreciate any help I can get form the list. > > Thank you, > Majid > > > > *Majid Ghoddusi, DVM, PhD* > *Veterinary Pathologist* > *Comparative Biosciences, Inc.* > *http://www.compbio.com/* > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From MVaughan4 <@t> ucok.edu Wed Jan 23 12:49:54 2008 From: MVaughan4 <@t> ucok.edu (MVaughan4@ucok.edu) Date: Wed Jan 23 12:50:42 2008 Subject: [Histonet] Sections falling off again In-Reply-To: Message-ID: Histonetters, I section skin and skin equivalents (artificial skin). I have antigens that require citrate buffer or protease XXIV/XXV retrieval. I often have trouble keeping the sections on the slides, whether I use Fisher or VWR superfrost plus or ProbeOn plus. Maybe they are not sticking initially. I have a water bath set at 42degreesC, the sections are about 8 microns, I put the slides overnight in a 37degree oven before refrigerating them. Does anybody see anything wrong with this? Thanks for your help, my students and I will be glad to get this fixed. Mel Melville B. Vaughan, Ph. D. Assistant Professor of Biology Campus Coordinator, NSF Sure-Step program University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm ----------------------------------------- **CONFIDENTIALITY** -This email (including any attachments) may contain confidential, proprietary and privileged information. Any unauthorized disclosure or use of this information is prohibited. From Robert.Lott <@t> TriadHospitals.com Wed Jan 23 14:19:37 2008 From: Robert.Lott <@t> TriadHospitals.com (Lott, Robert) Date: Wed Jan 23 14:20:00 2008 Subject: [Histonet] Olympus BH-2 microscope Message-ID: <518A08C53ED96D419F498D309E64A36A58FC66@CPRTEVS03.triadhospitals.net> I have an Olympus BH2 microscope, and the knob which controls the stage movement (up-down, left-right) needs replacing. I only need that one part! It is an Olympus BH2. Can anyone help? I would be interested in buying the part, or buying an old microscope to keep for scavenging (this and) future parts from... Thanks, Patty Lott CMBD Core Laboratory, UAB 205-934-2007 From settembr <@t> umdnj.edu Wed Jan 23 14:20:20 2008 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Wed Jan 23 14:21:12 2008 Subject: [Histonet] PMS2 Antibody Message-ID: Hello Jenny, I use and RUO PMS2 for Becton Dickinson Cat. 556415 I couldn't find an IVD I use it @ 1:10 with a labeled polymer detection kit. Good Luck, Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> "Bull, Jennifer L." 01/23/08 10:32 AM >>> I am looking for PMS2 antibody (clone A16-4) as an IVD for formalin fixed parafin embedded IHC. Is anyone out there using this or know where I can find the antibody? I am also looking for MLH1, MSH2 and MSH6, but I have a couple vendors for these so far. Any input is appreciated! Jenny _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Wed Jan 23 14:54:29 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed Jan 23 14:55:28 2008 Subject: [Histonet] Olympus BH-2 microscope In-Reply-To: <518A08C53ED96D419F498D309E64A36A58FC66@CPRTEVS03.triadhospitals.net> Message-ID: Contact www.Lukasmicroscope.com "Lott, Robert" Sent by: histonet-bounces@lists.utsouthwestern.edu 01/23/2008 02:19 PM To cc Subject [Histonet] Olympus BH-2 microscope I have an Olympus BH2 microscope, and the knob which controls the stage movement (up-down, left-right) needs replacing. I only need that one part! It is an Olympus BH2. Can anyone help? I would be interested in buying the part, or buying an old microscope to keep for scavenging (this and) future parts from... Thanks, Patty Lott CMBD Core Laboratory, UAB 205-934-2007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Wed Jan 23 15:09:58 2008 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Wed Jan 23 15:10:22 2008 Subject: [Histonet] Mucin containing cells too blue In-Reply-To: Message-ID: <295976.36473.qm@web31302.mail.mud.yahoo.com> Being the person who manufactured (CAT Hematoxylin) for Biocare, Extremely Large Volumes by the way, I would calibrate my pH meter daily using a #2 and #4 buffers. You also must make sure that your probe is designed for such a LOW pH. During my learning curve, I also found that any fluctuation in temperature, movement and also having the pH meter close to any magnetic fields, could alter the pH. What I mean is, if your procedure requires heat, the pH is quite different if it is slightly warm, rather than if it is at RT. I also found out that if my pH meter was located to closely between (2) magnetic stirrers. the pH would fluctuate. When manufacturing, I would keep the pH probe in the solution while it was being mixed. After putting a certain volume of GLACIAL ACETIC ACID in and mixing it, I would let the solution rest, then recheck the pH. I would repeat this procedure till I reached my desired pH. The CAT hematoxylin also has glycerol in the formula. This was added after pH'ing. The glycerol raises the pH slightly. There is also a proprietary stabilizer incorporated. Hope this helps and gives some insight. Akemi Allison-Tacha --- Tony Henwood wrote: > Good, > I assume you would check the pH before you put it on > the market. > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, > CT(ASC) > Laboratory Manager & Senior Scientist > The Children's Hospital at Westmead, > Locked Bag 4001, Westmead, 2145, AUSTRALIA. > Tel: 612 9845 3306 > Fax: 612 9845 3318 > > > > > -----Original Message----- > From: Akemi Allison-Tacha > [mailto:akemiat3377@yahoo.com] > Sent: Wednesday, 23 January 2008 8:25 AM > To: Tony Henwood; Greg Dobbin; > Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Mucin containing cells too > blue > > > The pH of the Hematoxylin I developed for Biocare > (CAT > Hematoxylin) a progressive hematoxylin, was between > pH > 2.26 to 2.3. I would let it oxidize (ripened) for 3 > months before releasing it for sale. It would not > over-stain mucin, especially in the GI tract. > > > --- Tony Henwood wrote: > > > Greg, > > > > Don't assume that "fresh hematoxylin (and > thererfore > > correct pH) would > > rectify the problem". Incorrect pH of the > > Haematoxylin is the usual > > problem. A feature of a haematoxylin solution that > > has a high pH is the > > staining of mucin, especially in the alimentary > > tract. Decreasing the pH > > of the staining solution to about 2.5 will > decrease > > this mucin staining. > > > > Regards > > > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, > > CT(ASC) > > Laboratory Manager & Senior Scientist > > The Children's Hospital at Westmead, > > Locked Bag 4001, Westmead, 2145, AUSTRALIA. > > Tel: 612 9845 3306 > > Fax: 612 9845 3318 > > > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] > > On Behalf Of Greg > > Dobbin > > Sent: Wednesday, 23 January 2008 7:24 AM > > To: Histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Mucin containing cells too > blue > > > > > > Hi Folks, > > I have been using the Surgipath Selectech reagents > > (H&E) for almost 3 > > months now and I have not had a single complaint > > about staining...until > > now. Suddenly the mucin containing cells are too > > blue. My first > > thought was to make fresh Define (the Surgipath > differentiator). This > > did not fix the problem. Next I replaced the > > hematoxylin with fresh > > (not supposed to have to change before 2500 > slides, > > but changed at 1500 > > slides to see if that would correct the problem). > It > > has not. > > > > I was sure that fresh hematoxylin (and thererfore > > correct pH) would > > rectify the problem. What are the other usual > > suspects here? I should > > also mention our H&E's are done on the Leica > > Autostainer. I look forward > > to receiving your collective insight! Greg > > > > > > > > Greg Dobbin, R.T. > > Chief Technologist, Histology Lab > > Dept. of Laboratory Medicine, > > Queen Elizabeth Hospital, > > P.O. Box 6600 > > Charlottetown, PE C1A 8T5 > > Phone: (902) 894-2337 > > Fax: (902) 894-2385 > > > > There is some merit in doing the right thing > rather > > badly, > > but absolutely none in doing the wrong thing > > excellently! > > > > Statement of Confidentiality > > This message (including attachments) may contain > > confidential or > > privileged information intended for a specific > > individual or > > organization. If you have received this > > communication in error, please > > notify the sender immediately. If you are not the > > intended recipient, > > you are not authorized to use, disclose, > distribute, > > copy, print or rely > > on this email, and should promptly delete this > email > > from your entire > > computer system. > > > > D?claration de confidentialit? > > Le pr?sent message (y compris les annexes) peut > > contenir des > > renseignements confidentiels ayant pour objet une > > personne ou un > > organisme particulier. Si vous avez re?u la > pr?sente communication par > > erreur, veuillez en informer l'exp?diteur > > imm?diatement. Si vous n'?tes > > pas le destinataire pr?vu, vous n'avez pas le > droit > > d'utiliser, > > divulguer, distribuer, copier ou imprimer ce > > courriel ou encore de vous > > en servir, et vous devriez l'effacer compl?tement > de > > votre syst?me > > informatique. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ********************************************************************* > > This email and any files transmitted with it are > > confidential and intended solely for the use of > the individual or > > entity to whom they are addressed. If you are not > the intended > > recipient, please delete it and notify the sender. > > > > Views expressed in this message and any > attachments > > are those of the individual sender, and are not > > necessarily the views of The Children's Hospital > at > > Westmead > > > > This note also confirms that this email message > has > > been > > virus scanned and although no computer viruses > were > > detected, The Childrens Hospital at Westmead > accepts > > no liability for any consequential damage > resulting > > from email containing computer viruses. > > > ********************************************************************** > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > Akemi Allison-Tacha, BS, HT(ASCP)HTL > === message truncated === Akemi Allison-Tacha, BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing Tele: (925)788-0900 E-Mail: akemiat3377@yahoo.com From as3323 <@t> columbia.edu Wed Jan 23 21:31:01 2008 From: as3323 <@t> columbia.edu (as3323@columbia.edu) Date: Wed Jan 23 21:31:24 2008 Subject: [Histonet] Herxheimer's Stain Message-ID: <20080123223101.j6za0qq6o8wk4w40@cubmail.cc.columbia.edu> Hello Histonet, A colleague has suggested that I do some research on Herxheimer's stain. Nothing turned up in the archives, so I hope someone can help me. She was interested in its properties for staining sebaceous glands along with making hair visible (esp. in whole mounts). We know that oil red is used for this too. Does anyone have any info on which would be better for what? Much of the work I do is focused on hair follicles, so this would be valuable info. thanks, (I also apologize for any funny phrasing or bad grammar as I am a bit under the weather right now) Anna Core Tech (Dermatology Basic Science Research) Columbia Univ Med Center From laurie.reilly <@t> jcu.edu.au Wed Jan 23 22:43:28 2008 From: laurie.reilly <@t> jcu.edu.au (Laurie Reilly) Date: Wed Jan 23 22:45:11 2008 Subject: [Histonet] Herxheimer's Stain In-Reply-To: <20080123223101.j6za0qq6o8wk4w40@cubmail.cc.columbia.edu> References: <20080123223101.j6za0qq6o8wk4w40@cubmail.cc.columbia.edu> Message-ID: <000c01c85e43$aa65d7c0$5255db89@health.ad.jcu.edu.au> Dear Anna and Histonetters, Herxheimer's Fat stain was taught to us in my training days at Royal Melbourne Institute of Technology - I still use it occasionally and teach it to our Med Lab Science students. The method, and I quote from "Histological Methods" compiled by J.Blake and staff. "Mix equal quantities of dry Sudan III, IV and Oil Red O powder in a clear dry bottle. Add a mixture of equal volumes of acetone and 70% ethanol and shake well. Leave solution to saturate a few days. For use: Pipette supernatant without disturbing precipitate. When using, cover vessel to avoid evaporation and precipitation of stain." The method is to stain frozen sections with Mayer's haematoxylin, blue, rinse in 70% ethanol, stain in Herxheimer's for 1 minute, rinse in 70% ethanol then water and mount in an aqueous mounting medium. The reference given is:- Herxheimer, Zbl. Allg. Path., 14, 891, 1903. (Probably a bit early for our archives.) Regards, Laurie. Mr. Laurie REILLY Histopathology School of Veterinary and Biomedical Sciences James Cook University Townsville Qld. 4811 Australia. Phone 07 4781 4468 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of as3323@columbia.edu Sent: Thursday, 24 January 2008 1:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Herxheimer's Stain Hello Histonet, A colleague has suggested that I do some research on Herxheimer's stain. Nothing turned up in the archives, so I hope someone can help me. She was interested in its properties for staining sebaceous glands along with making hair visible (esp. in whole mounts). We know that oil red is used for this too. Does anyone have any info on which would be better for what? Much of the work I do is focused on hair follicles, so this would be valuable info. thanks, (I also apologize for any funny phrasing or bad grammar as I am a bit under the weather right now) Anna Core Tech (Dermatology Basic Science Research) Columbia Univ Med Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joost.bruijntjes <@t> tno.nl Thu Jan 24 03:10:27 2008 From: joost.bruijntjes <@t> tno.nl (Bruijntjes, J.P. (Joost)) Date: Thu Jan 24 03:10:57 2008 Subject: [Histonet] CD25 - Rat Message-ID: <8865601DD17A554CB489C17FFD8A51B2CDDFE2@MAIL04.tsn.tno.nl> Hi all Is anyone of you familiar with staining rat lymphoid tissues with an antibody directed against CD25? In the white pulp there is a lot of positive staining on different lymphocytes in the PALS, (less in the) B-cell area. In the marginal zone positive staining is seen on some large cells, most probably macrophages. In the red pulp there is a lot of staining on different type of lymphocytes too. But I see a lot of positive staining on (most probably) capillaries (endothelial cells) . Not a few capillaries, but all. Does it sound familiar to anyone of you? Joost Bruijntjes TNO Quality of Life Zeist The Netherlands TNO.NL Joost Bruijntjes T +31 30 694 44 80 F +31 30 694 49 86 E joost.bruijntjes@tno.nl Disclaimer This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html From rjbuesa <@t> yahoo.com Thu Jan 24 07:58:43 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 24 07:59:08 2008 Subject: [Histonet] Mucin containing cells too blue In-Reply-To: <295976.36473.qm@web31302.mail.mud.yahoo.com> Message-ID: <808308.63493.qm@web61215.mail.yahoo.com> Just a note! The magnetic field does NOT alter the pH, it alters the pH-meter galvanometer that will read out a wrong pH value. Ren? J. Akemi Allison-Tacha wrote: Being the person who manufactured (CAT Hematoxylin) for Biocare, Extremely Large Volumes by the way, I would calibrate my pH meter daily using a #2 and #4 buffers. You also must make sure that your probe is designed for such a LOW pH. During my learning curve, I also found that any fluctuation in temperature, movement and also having the pH meter close to any magnetic fields, could alter the pH. What I mean is, if your procedure requires heat, the pH is quite different if it is slightly warm, rather than if it is at RT. I also found out that if my pH meter was located to closely between (2) magnetic stirrers. the pH would fluctuate. When manufacturing, I would keep the pH probe in the solution while it was being mixed. After putting a certain volume of GLACIAL ACETIC ACID in and mixing it, I would let the solution rest, then recheck the pH. I would repeat this procedure till I reached my desired pH. The CAT hematoxylin also has glycerol in the formula. This was added after pH'ing. The glycerol raises the pH slightly. There is also a proprietary stabilizer incorporated. Hope this helps and gives some insight. Akemi Allison-Tacha --- Tony Henwood wrote: > Good, > I assume you would check the pH before you put it on > the market. > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, > CT(ASC) > Laboratory Manager & Senior Scientist > The Children's Hospital at Westmead, > Locked Bag 4001, Westmead, 2145, AUSTRALIA. > Tel: 612 9845 3306 > Fax: 612 9845 3318 > > > > > -----Original Message----- > From: Akemi Allison-Tacha > [mailto:akemiat3377@yahoo.com] > Sent: Wednesday, 23 January 2008 8:25 AM > To: Tony Henwood; Greg Dobbin; > Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Mucin containing cells too > blue > > > The pH of the Hematoxylin I developed for Biocare > (CAT > Hematoxylin) a progressive hematoxylin, was between > pH > 2.26 to 2.3. I would let it oxidize (ripened) for 3 > months before releasing it for sale. It would not > over-stain mucin, especially in the GI tract. > > > --- Tony Henwood wrote: > > > Greg, > > > > Don't assume that "fresh hematoxylin (and > thererfore > > correct pH) would > > rectify the problem". Incorrect pH of the > > Haematoxylin is the usual > > problem. A feature of a haematoxylin solution that > > has a high pH is the > > staining of mucin, especially in the alimentary > > tract. Decreasing the pH > > of the staining solution to about 2.5 will > decrease > > this mucin staining. > > > > Regards > > > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, > > CT(ASC) > > Laboratory Manager & Senior Scientist > > The Children's Hospital at Westmead, > > Locked Bag 4001, Westmead, 2145, AUSTRALIA. > > Tel: 612 9845 3306 > > Fax: 612 9845 3318 > > > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] > > On Behalf Of Greg > > Dobbin > > Sent: Wednesday, 23 January 2008 7:24 AM > > To: Histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Mucin containing cells too > blue > > > > > > Hi Folks, > > I have been using the Surgipath Selectech reagents > > (H&E) for almost 3 > > months now and I have not had a single complaint > > about staining...until > > now. Suddenly the mucin containing cells are too > > blue. My first > > thought was to make fresh Define (the Surgipath > differentiator). This > > did not fix the problem. Next I replaced the > > hematoxylin with fresh > > (not supposed to have to change before 2500 > slides, > > but changed at 1500 > > slides to see if that would correct the problem). > It > > has not. > > > > I was sure that fresh hematoxylin (and thererfore > > correct pH) would > > rectify the problem. What are the other usual > > suspects here? I should > > also mention our H&E's are done on the Leica > > Autostainer. I look forward > > to receiving your collective insight! Greg > > > > > > > > Greg Dobbin, R.T. > > Chief Technologist, Histology Lab > > Dept. of Laboratory Medicine, > > Queen Elizabeth Hospital, > > P.O. Box 6600 > > Charlottetown, PE C1A 8T5 > > Phone: (902) 894-2337 > > Fax: (902) 894-2385 > > > > There is some merit in doing the right thing > rather > > badly, > > but absolutely none in doing the wrong thing > > excellently! > > > > Statement of Confidentiality > > This message (including attachments) may contain > > confidential or > > privileged information intended for a specific > > individual or > > organization. If you have received this > > communication in error, please > > notify the sender immediately. If you are not the > > intended recipient, > > you are not authorized to use, disclose, > distribute, > > copy, print or rely > > on this email, and should promptly delete this > email > > from your entire > > computer system. > > > > D?claration de confidentialit? > > Le pr?sent message (y compris les annexes) peut > > contenir des > > renseignements confidentiels ayant pour objet une > > personne ou un > > organisme particulier. Si vous avez re?u la > pr?sente communication par > > erreur, veuillez en informer l'exp?diteur > > imm?diatement. Si vous n'?tes > > pas le destinataire pr?vu, vous n'avez pas le > droit > > d'utiliser, > > divulguer, distribuer, copier ou imprimer ce > > courriel ou encore de vous > > en servir, et vous devriez l'effacer compl?tement > de > > votre syst?me > > informatique. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ********************************************************************* > > This email and any files transmitted with it are > > confidential and intended solely for the use of > the individual or > > entity to whom they are addressed. If you are not > the intended > > recipient, please delete it and notify the sender. > > > > Views expressed in this message and any > attachments > > are those of the individual sender, and are not > > necessarily the views of The Children's Hospital > at > > Westmead > > > > This note also confirms that this email message > has > > been > > virus scanned and although no computer viruses > were > > detected, The Childrens Hospital at Westmead > accepts > > no liability for any consequential damage > resulting > > from email containing computer viruses. > > > ********************************************************************** > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > Akemi Allison-Tacha, BS, HT(ASCP)HTL > === message truncated === Akemi Allison-Tacha, BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing Tele: (925)788-0900 E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From mona_diane <@t> hotmail.com Thu Jan 24 08:24:46 2008 From: mona_diane <@t> hotmail.com (Ramona Turner) Date: Thu Jan 24 08:25:14 2008 Subject: [Histonet] HER2neu Fixation times Message-ID: This is a followup to a recent question asking what do small histology labs do about proper fixation time for HER2neu if there is no weekend rotation...... I would also like to know....other than having a rotation tech to start the processor on Saturday, I can't think of a solution....A Saturday rotation would be a great hardship for three techs to maintain, plus that is one of the perks of the job is not having to work weekends. Any ideas? Ramona Turner, HT (ASCP) Histology Supervisor POTOMAC HOSPITAL 2300 Opitz Blvd Woodbridge, VA 22191 _________________________________________________________________ Helping your favorite cause is as easy as instant messaging.?You IM, we give. http://im.live.com/Messenger/IM/Home/?source=text_hotmail_join From rjbuesa <@t> yahoo.com Thu Jan 24 08:28:39 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 24 08:29:07 2008 Subject: [Histonet] HER2neu Fixation times In-Reply-To: Message-ID: <624582.7376.qm@web61217.mail.yahoo.com> You can always set your tissue processor to start ON DELAY mode and it will start automatically without the need of a weekend rotation. Ren? J. Ramona Turner wrote: This is a followup to a recent question asking what do small histology labs do about proper fixation time for HER2neu if there is no weekend rotation...... I would also like to know....other than having a rotation tech to start the processor on Saturday, I can't think of a solution....A Saturday rotation would be a great hardship for three techs to maintain, plus that is one of the perks of the job is not having to work weekends. Any ideas? Ramona Turner, HT (ASCP) Histology Supervisor POTOMAC HOSPITAL 2300 Opitz Blvd Woodbridge, VA 22191 _________________________________________________________________ Helping your favorite cause is as easy as instant messaging. You IM, we give. http://im.live.com/Messenger/IM/Home/?source=text_hotmail_join_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From mona_diane <@t> hotmail.com Thu Jan 24 08:38:44 2008 From: mona_diane <@t> hotmail.com (Ramona Turner) Date: Thu Jan 24 08:39:08 2008 Subject: [Histonet] How to bill for additional testing on old cases Message-ID: Currently, in order to bill for additional testing that is ordered on old cases, we must have the patient re-register and open a new billing number. Then in CoPath, we give the old case a new surgical number. A gross descriptions is given.... "Case S08-x is patient name John Doe. Received is one paraffin block labeled S02-x-J sent for additional testing for Her2neu." This puts the new surgical number on the pathologist's work list to sign out later. This is the only way we can think of to bill for the additional testing. The problem is that now the one case has two surgical numbers and the additional testing results are dictated on the new surgical number, not the old one. IF the old case is ever pulled again for review, the new results will not be shown unless they look at the new surgical number. The pathologist are not happy about this.... IF anyone is doing things different, PLEASE respond........ Ramona Turner, HT (ASCP) POTOMAC HOSPITAL 2300 Opitz Blvd Woodbridge, VA 22191 703-670-1341 _________________________________________________________________ Helping your favorite cause is as easy as instant messaging.?You IM, we give. http://im.live.com/Messenger/IM/Home/?source=text_hotmail_join From gvdobbin <@t> ihis.org Thu Jan 24 08:41:52 2008 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Thu Jan 24 08:42:27 2008 Subject: [Histonet] HER2neu Fixation times Message-ID: Delaying start time of the processor does not address the issue of the tissues fixing in formalin for greater than 48 hours! Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 There is some merit in doing the right thing rather badly, but absolutely none in doing the wrong thing excellently! >>> Ramona Turner 1/24/2008 10:24 AM >>> This is a followup to a recent question asking what do small histology labs do about proper fixation time for HER2neu if there is no weekend rotation...... I would also like to know....other than having a rotation tech to start the processor on Saturday, I can't think of a solution....A Saturday rotation would be a great hardship for three techs to maintain, plus that is one of the perks of the job is not having to work weekends. Any ideas? Ramona Turner, HT (ASCP) Histology Supervisor POTOMAC HOSPITAL 2300 Opitz Blvd Woodbridge, VA 22191 _________________________________________________________________ Helping your favorite cause is as easy as instant messaging. You IM, we give. http://im.live.com/Messenger/IM/Home/?source=text_hotmail_join_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ayant pour objet une personne ou un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer compl?tement de votre syst?me informatique. From rjbuesa <@t> yahoo.com Thu Jan 24 08:46:09 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 24 08:46:35 2008 Subject: [Histonet] HER2neu Fixation times In-Reply-To: Message-ID: <786714.18940.qm@web61211.mail.yahoo.com> Yes it does! If you receive the specimen on a Friday and set the tissue processor to start accordingly the tissue will be fixed within the time limits. Ren? J. Greg Dobbin wrote: Delaying start time of the processor does not address the issue of the tissues fixing in formalin for greater than 48 hours! Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 There is some merit in doing the right thing rather badly, but absolutely none in doing the wrong thing excellently! >>> Ramona Turner 1/24/2008 10:24 AM >>> This is a followup to a recent question asking what do small histology labs do about proper fixation time for HER2neu if there is no weekend rotation...... I would also like to know....other than having a rotation tech to start the processor on Saturday, I can't think of a solution....A Saturday rotation would be a great hardship for three techs to maintain, plus that is one of the perks of the job is not having to work weekends. Any ideas? Ramona Turner, HT (ASCP) Histology Supervisor POTOMAC HOSPITAL 2300 Opitz Blvd Woodbridge, VA 22191 _________________________________________________________________ Helping your favorite cause is as easy as instant messaging. You IM, we give. http://im.live.com/Messenger/IM/Home/?source=text_hotmail_join_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ayant pour objet une personne ou un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer compl?tement de votre syst?me informatique. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From JWEEMS <@t> sjha.org Thu Jan 24 08:47:17 2008 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Thu Jan 24 08:47:45 2008 Subject: [Histonet] How to bill for additional testing on old cases References: Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3204726BB4@sjhaexc02.sjha.org> I can see how that would be dangerous! Can you bill in the hospital system without accessioning a case? If not I would add a case type to use for bill only cases and add a note on the new number such as "For billing only". I would do an addendum on the original case for the results. (I bill directly into our hospital IDX system in these instances.) Good luck! Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Ramona Turner Sent: Thu 1/24/2008 9:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How to bill for additional testing on old cases Currently, in order to bill for additional testing that is ordered on old cases, we must have the patient re-register and open a new billing number. Then in CoPath, we give the old case a new surgical number. A gross descriptions is given.... "Case S08-x is patient name John Doe. Received is one paraffin block labeled S02-x-J sent for additional testing for Her2neu." This puts the new surgical number on the pathologist's work list to sign out later. This is the only way we can think of to bill for the additional testing. The problem is that now the one case has two surgical numbers and the additional testing results are dictated on the new surgical number, not the old one. IF the old case is ever pulled again for review, the new results will not be shown unless they look at the new surgical number. The pathologist are not happy about this.... IF anyone is doing things different, PLEASE respond........ Ramona Turner, HT (ASCP) POTOMAC HOSPITAL 2300 Opitz Blvd Woodbridge, VA 22191 703-670-1341 _________________________________________________________________ Helping your favorite cause is as easy as instant messaging. You IM, we give. http://im.live.com/Messenger/IM/Home/?source=text_hotmail_join_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From jmahoney <@t> alegent.org Thu Jan 24 08:51:55 2008 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Thu Jan 24 08:52:39 2008 Subject: [Histonet] HER2neu Fixation times In-Reply-To: <624582.7376.qm@web61217.mail.yahoo.com> References: <624582.7376.qm@web61217.mail.yahoo.com> Message-ID: <479851AB0200003C00028D7A@gwia.alegent.org> Ramona, I understand your dilemma. We do have coverage on Saturdays but will run into the same problem on three day weekends. (The issue being that Her-2-IHC should be fixed no longer than 48 hours to be valid.) We will follow the criteria and if the fixation is longer than 48 hours and HER-2-neu by IHC is positive, it is ok to report that result. If it is negative we will reflex to FISH. I'd love to hear from more people as to what their plan is. Jan Mahoney Omaha, NE >>> Rene J Buesa 01/24/2008 8:28 AM >>> You can always set your tissue processor to start ON DELAY mode and it will start automatically without the need of a weekend rotation. Ren? J. Ramona Turner wrote: This is a followup to a recent question asking what do small histology labs do about proper fixation time for HER2neu if there is no weekend rotation...... I would also like to know....other than having a rotation tech to start the processor on Saturday, I can't think of a solution....A Saturday rotation would be a great hardship for three techs to maintain, plus that is one of the perks of the job is not having to work weekends. Any ideas? Ramona Turner, HT (ASCP) Histology Supervisor POTOMAC HOSPITAL 2300 Opitz Blvd Woodbridge, VA 22191 _________________________________________________________________ Helping your favorite cause is as easy as instant messaging. You IM, we give. http://im.live.com/Messenger/IM/Home/?source=text_hotmail_join_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Robinsoc <@t> mercyhealth.com Thu Jan 24 08:52:19 2008 From: Robinsoc <@t> mercyhealth.com (Cynthia Robinson) Date: Thu Jan 24 08:53:09 2008 Subject: [Histonet] How to bill for additional testing on old cases In-Reply-To: References: Message-ID: <479851C3.59BC.00AF.0@mercyhealth.com> We give the patient a new billing number but we order everything on the original pathology accession number. We manually move the billing using Cerner LIS system from the old billing number to the new one. CoPath is part of Cerner isn't it? Perhaps there is a way to electronically move the billing to the new number like we do in Millennium. You can contact me off list if you like. Cindi Robinson HT(ASCP) MMC-Sioux City Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 712-279-2768 >>> Ramona Turner 1/24/2008 8:38 AM >>> Currently, in order to bill for additional testing that is ordered on old cases, we must have the patient re-register and open a new billing number. Then in CoPath, we give the old case a new surgical number. A gross descriptions is given.... "Case S08-x is patient name John Doe. Received is one paraffin block labeled S02-x-J sent for additional testing for Her2neu." This puts the new surgical number on the pathologist's work list to sign out later. This is the only way we can think of to bill for the additional testing. The problem is that now the one case has two surgical numbers and the additional testing results are dictated on the new surgical number, not the old one. IF the old case is ever pulled again for review, the new results will not be shown unless they look at the new surgical number. The pathologist are not happy about this.... IF anyone is doing things different, PLEASE respond........ Ramona Turner, HT (ASCP) POTOMAC HOSPITAL 2300 Opitz Blvd Woodbridge, VA 22191 703-670-1341 _________________________________________________________________ Helping your favorite cause is as easy as instant messaging. You IM, we give. http://im.live.com/Messenger/IM/Home/?source=text_hotmail_join_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Thu Jan 24 08:57:05 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Jan 24 08:57:37 2008 Subject: [Histonet] HER2neu Fixation times In-Reply-To: References: Message-ID: <479860F2020000770000A527@gwmail4.harthosp.org> Personally, I would not initiate any drastic changes at this point. Keep in mind that these are guidelines; however, you must validate your testing if you are going to follow other fixation guidelines. I think everyone knowledgeable with this issue knows that the problem is with underfixation, not overfixation. I recently pulled tumor out of formalin after 8 months of fixation and the IHC was still "3+" and the FISH showed beautiful amplification (ratio of 10.0). I hope that once the scientific evidence is evaluated, these guidelines will be changed. Major expense is being incurred here unnecessarily. How is your concordance between IHC and FISH for the detection of HER2? If it's not broken, don't try to fix it. Our "ad-hoc" committee on IHC standardization is meeting in Santa Barbara on Sunday and I hope this issue will be discussed. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> Ramona Turner 01/24/08 9:24 AM >>> This is a followup to a recent question asking what do small histology labs do about proper fixation time for HER2neu if there is no weekend rotation...... I would also like to know....other than having a rotation tech to start the processor on Saturday, I can't think of a solution....A Saturday rotation would be a great hardship for three techs to maintain, plus that is one of the perks of the job is not having to work weekends. Any ideas? Ramona Turner, HT (ASCP) Histology Supervisor POTOMAC HOSPITAL 2300 Opitz Blvd Woodbridge, VA 22191 _________________________________________________________________ Helping your favorite cause is as easy as instant messaging. You IM, we give. http://im.live.com/Messenger/IM/Home/?source=text_hotmail_join_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From Lynne.Bell <@t> hitchcock.org Thu Jan 24 09:07:55 2008 From: Lynne.Bell <@t> hitchcock.org (Bell, Lynne) Date: Thu Jan 24 09:08:16 2008 Subject: [Histonet] How to bill for additional testing on old cases In-Reply-To: Message-ID: Ramona, If CoPath allows you to use a different prefix, you could do as we do when using Meditech. I use the prefix SS (special studies) and I assign the same case number to the new case. The slides are then filed with the original case number. I had our LIS coordinator help with the computer end of it, such as making the prefix and also making the case number not automatically assigned. Hope this helps. Lynne Bell, HT (ASCP) Central Vermont Medical Center 130 Fisher Road Barre, VT 05641 802-371-4923 From Gguerzon <@t> lifebridgehealth.org Thu Jan 24 09:07:25 2008 From: Gguerzon <@t> lifebridgehealth.org (Godfrey Guerzon) Date: Thu Jan 24 09:08:59 2008 Subject: [Histonet] HER2neu Fixation times Message-ID: We decided to validate fixation of up to 96 hours (to cover long weekend). From each case we set aside a tissue from the tumor and fix the tissue for 96 hours before processing. Then we run Her2-Neu on a block that was fixed between 6-48 hours and the one fixed for 96 hours from the same case and compare the results. We are doing this for about 20-25 cases, keep the documentation. We have not completed the validation but so far we are not observing any difference in the staining. This should take care of the fixation issue. Godfrey >>> "Janice Mahoney" 1/24/2008 9:51 AM >>> Ramona, I understand your dilemma. We do have coverage on Saturdays but will run into the same problem on three day weekends. (The issue being that Her-2-IHC should be fixed no longer than 48 hours to be valid.) We will follow the criteria and if the fixation is longer than 48 hours and HER-2-neu by IHC is positive, it is ok to report that result. If it is negative we will reflex to FISH. I'd love to hear from more people as to what their plan is. Jan Mahoney Omaha, NE >>> Rene J Buesa 01/24/2008 8:28 AM >>> You can always set your tissue processor to start ON DELAY mode and it will start automatically without the need of a weekend rotation. Ren? J. Ramona Turner wrote: This is a followup to a recent question asking what do small histology labs do about proper fixation time for HER2neu if there is no weekend rotation...... I would also like to know....other than having a rotation tech to start the processor on Saturday, I can't think of a solution....A Saturday rotation would be a great hardship for three techs to maintain, plus that is one of the perks of the job is not having to work weekends. Any ideas? Ramona Turner, HT (ASCP) Histology Supervisor POTOMAC HOSPITAL 2300 Opitz Blvd Woodbridge, VA 22191 _________________________________________________________________ Helping your favorite cause is as easy as instant messaging. You IM, we give. http://im.live.com/Messenger/IM/Home/?source=text_hotmail_join_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------------------------------- THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE ADDRESSEE LISTED ABOVE. This record has been disclosed in accordance with Subtitle 3 of Title 4 of the Health-General Article of the Annotated Code of Maryland. Further disclosure of medical information contained herein is prohibited. If you are neither the intended recipient nor the individual responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure of patient information is strictly prohibited. If you have received this email in error, immediately notify us by telephone or return email. ----------------------------------------------------------------- From dgrant3 <@t> kumc.edu Thu Jan 24 09:55:33 2008 From: dgrant3 <@t> kumc.edu (Debra Grant) Date: Thu Jan 24 09:56:13 2008 Subject: [Histonet] Z-Fix Message-ID: <47986095.D0C5.00BA.0@kumc.edu> Hi all, Does anyone have experience using Z-Fix to fix skin tissue before transferring to 10%NBF on the tissue processor? Thank you in advance, Debby Grant From gu.lang <@t> gmx.at Thu Jan 24 10:14:41 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Jan 24 10:15:06 2008 Subject: [Histonet] papanicolaou on leica stainer Message-ID: <001501c85ea4$3a3ad200$eeeea8c0@dielangs.at> Hi! Has anybody experience with the papanicolaou stain on the new leica stainer? Can anybody share the protocol with me? Thank you in advance Gudrun From ancillarypath <@t> mac.com Thu Jan 24 10:19:42 2008 From: ancillarypath <@t> mac.com (ancillarypath@mac.com) Date: Thu Jan 24 10:20:07 2008 Subject: [Histonet] Re: HER2 fixation time Message-ID: <9C02888C-455A-448B-BF3F-6B722BBF871B@mac.com> I agree with Rich, and it's good to hear that some colleagues have started their own mode of cross-validation. If you choose to deviate from the upper fixation limit of 48 hours, you will ONLY be at default if you do not have evidence (with documentation) that raising the upper fixation limits to 72 or 96 hours has no detrimental effects on the results. The CAP will eventually increase the upper limit to 72 (or hopefully 96) hours once there is solid evidence that is ok to do so. Rene's suggestion to put the instrument on delay is not a valid solution. As long as the tissue is sitting in formalin in the instrument while it's on delay, it's still being fixed. This issue was discussed at the ASCO/CAP meeting. Hadi ============================== Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories, President, Ancillary Pathways 7000 SW 62nd Avenue, Suite Penthouse-C Miami, FL 33143 Tel 305.740.4440 Fax 786.513.0175 www.vitromolecular.com www.ancillarypath.com This email message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e- mail and destroy all copies of the original message. Message: 18 Date: Thu, 24 Jan 2008 09:57:05 -0500 From: "Richard Cartun" Subject: Re: [Histonet] HER2neu Fixation times To: "Ramona Turner" , Message-ID: <479860F2020000770000A527@gwmail4.harthosp.org> Content-Type: text/plain; charset=US-ASCII Personally, I would not initiate any drastic changes at this point. Keep in mind that these are guidelines; however, you must validate your testing if you are going to follow other fixation guidelines. I think everyone knowledgeable with this issue knows that the problem is with underfixation, not overfixation. I recently pulled tumor out of formalin after 8 months of fixation and the IHC was still "3+" and the FISH showed beautiful amplification (ratio of 10.0). I hope that once the scientific evidence is evaluated, these guidelines will be changed. Major expense is being incurred here unnecessarily. How is your concordance between IHC and FISH for the detection of HER2? If it's not broken, don't try to fix it. Our "ad-hoc" committee on IHC standardization is meeting in Santa Barbara on Sunday and I hope this issue will be discussed. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From b-frederick <@t> northwestern.edu Thu Jan 24 10:20:37 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Jan 24 10:21:10 2008 Subject: [Histonet] HER2neu Fixation times In-Reply-To: <479851AB0200003C00028D7A@gwia.alegent.org> Message-ID: <000101c85ea5$11996720$d00f7ca5@lurie.northwestern.edu> ALL, You can fix that tissue for 48 hours and have it start from 70% or adjust your processor to do so. That alleviates the time problem. We have researchers do this all the time particularly if they or we (to validate a test) are doing a time-point fixation. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Thursday, January 24, 2008 8:52 AM To: Ramona Turner; histonet@lists.utsouthwestern.edu; Rene J Buesa Subject: Re: [Histonet] HER2neu Fixation times Ramona, I understand your dilemma. We do have coverage on Saturdays but will run into the same problem on three day weekends. (The issue being that Her-2-IHC should be fixed no longer than 48 hours to be valid.) We will follow the criteria and if the fixation is longer than 48 hours and HER-2-neu by IHC is positive, it is ok to report that result. If it is negative we will reflex to FISH. I'd love to hear from more people as to what their plan is. Jan Mahoney Omaha, NE >>> Rene J Buesa 01/24/2008 8:28 AM >>> You can always set your tissue processor to start ON DELAY mode and it will start automatically without the need of a weekend rotation. Ren? J. Ramona Turner wrote: This is a followup to a recent question asking what do small histology labs do about proper fixation time for HER2neu if there is no weekend rotation...... I would also like to know....other than having a rotation tech to start the processor on Saturday, I can't think of a solution....A Saturday rotation would be a great hardship for three techs to maintain, plus that is one of the perks of the job is not having to work weekends. Any ideas? Ramona Turner, HT (ASCP) Histology Supervisor POTOMAC HOSPITAL 2300 Opitz Blvd Woodbridge, VA 22191 _________________________________________________________________ Helping your favorite cause is as easy as instant messaging. You IM, we give. http://im.live.com/Messenger/IM/Home/?source=text_hotmail_join______________ _________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Jan 24 10:46:40 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 24 10:47:03 2008 Subject: [Histonet] Re: HER2 fixation time In-Reply-To: <9C02888C-455A-448B-BF3F-6B722BBF871B@mac.com> Message-ID: <325597.93538.qm@web61211.mail.yahoo.com> Not if you set the instrument to start EXACTLY when you want. You will have the tissues in melted paraffin more time, or you can extend the dehydration times. You can do a lot of things all preventing longer fixation in NBF. Modern TP are very flexible instruments and I have no data that reflects any deleterious effects for the tissues' reactive qualities or sectioning characteristics after being in melted paraffin for long periods of time. Ren? J. ancillarypath@mac.com wrote: I agree with Rich, and it's good to hear that some colleagues have started their own mode of cross-validation. If you choose to deviate from the upper fixation limit of 48 hours, you will ONLY be at default if you do not have evidence (with documentation) that raising the upper fixation limits to 72 or 96 hours has no detrimental effects on the results. The CAP will eventually increase the upper limit to 72 (or hopefully 96) hours once there is solid evidence that is ok to do so. Rene's suggestion to put the instrument on delay is not a valid solution. As long as the tissue is sitting in formalin in the instrument while it's on delay, it's still being fixed. This issue was discussed at the ASCO/CAP meeting. Hadi ============================== Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories, President, Ancillary Pathways 7000 SW 62nd Avenue, Suite Penthouse-C Miami, FL 33143 Tel 305.740.4440 Fax 786.513.0175 www.vitromolecular.com www.ancillarypath.com This email message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e- mail and destroy all copies of the original message. Message: 18 Date: Thu, 24 Jan 2008 09:57:05 -0500 From: "Richard Cartun" Subject: Re: [Histonet] HER2neu Fixation times To: "Ramona Turner" , Message-ID: <479860F2020000770000A527@gwmail4.harthosp.org> Content-Type: text/plain; charset=US-ASCII Personally, I would not initiate any drastic changes at this point. Keep in mind that these are guidelines; however, you must validate your testing if you are going to follow other fixation guidelines. I think everyone knowledgeable with this issue knows that the problem is with underfixation, not overfixation. I recently pulled tumor out of formalin after 8 months of fixation and the IHC was still "3+" and the FISH showed beautiful amplification (ratio of 10.0). I hope that once the scientific evidence is evaluated, these guidelines will be changed. Major expense is being incurred here unnecessarily. How is your concordance between IHC and FISH for the detection of HER2? If it's not broken, don't try to fix it. Our "ad-hoc" committee on IHC standardization is meeting in Santa Barbara on Sunday and I hope this issue will be discussed. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From ancillarypath <@t> mac.com Thu Jan 24 11:07:58 2008 From: ancillarypath <@t> mac.com (Dr. Hadi Yaziji) Date: Thu Jan 24 11:08:24 2008 Subject: [Histonet] Re: HER2 fixation time In-Reply-To: <325597.93538.qm@web61211.mail.yahoo.com> References: <325597.93538.qm@web61211.mail.yahoo.com> Message-ID: <50F106F7-994E-4050-9511-DDC8A0743DA1@mac.com> This is a very good point, Rene. However, ... Prolonged exposure to melted paraffin (in 60 degrees) may be as bad (or most likely worse) than overfixing in formalin. Each time a standard parameter is drastically changed (such as over- exposure to dehydration or melted paraffin), that component NEEDS to be validated when it comes to predictive markers assay. I'd much rather validate 72-hr fixation than having to validate prolonged exposure to components of the tissue processor. Immunohistochemistry of predictive markers needs to be treated differently than diagnostic markers. It should be done with maximum care so we can tell the oncology community that the results our labs are producing are reliable and trustworthy. This is the essence of HER2 guidelines. The minute we start deviating from a pre-analytical parameter, the minute we start getting into trouble. In fact, if people have been fixing their speciment according to the FDA-approved fixation range of 6-24 hours, we wouldn't have been in the mess we're in right now. Hadi On Jan 24, 2008, at 11:46 AM, Rene J Buesa wrote: > Not if you set the instrument to start EXACTLY when you want. You > will have the tissues in melted paraffin more time, or you can > extend the dehydration times. You can do a lot of things all > preventing longer fixation in NBF. Modern TP are very flexible > instruments and I have no data that reflects any deleterious effects > for the tissues' reactive qualities or sectioning characteristics > after being in melted paraffin for long periods of time. > Ren? J. > > ancillarypath@mac.com wrote: > I agree with Rich, and it's good to hear that some colleagues have > started their own mode of cross-validation. > > If you choose to deviate from the upper fixation limit of 48 hours, > you will ONLY be at default if you do not have evidence (with > documentation) that raising the upper fixation limits to 72 or 96 > hours has no detrimental effects on the results. The CAP will > eventually increase the upper limit to 72 (or hopefully 96) hours once > there is solid evidence that is ok to do so. > > Rene's suggestion to put the instrument on delay is not a valid > solution. As long as the tissue is sitting in formalin in the > instrument while it's on delay, it's still being fixed. This issue was > discussed at the ASCO/CAP meeting. > > Hadi > > ============================== > Hadi Yaziji, M.D., Medical Director > Vitro Molecular Laboratories, > President, Ancillary Pathways > 7000 SW 62nd Avenue, Suite Penthouse-C > Miami, FL 33143 > Tel 305.740.4440 > Fax 786.513.0175 > www.vitromolecular.com > www.ancillarypath.com > > This email message, including any attachments, is for the sole use of > the intended recipients and may contain privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. > If you are not the intended recipient, please contact the sender by e- > mail and destroy all copies of the original message. > > > Message: 18 > Date: Thu, 24 Jan 2008 09:57:05 -0500 > From: "Richard Cartun" > Subject: Re: [Histonet] HER2neu Fixation times > To: "Ramona Turner" , > > Message-ID: <479860F2020000770000A527@gwmail4.harthosp.org> > Content-Type: text/plain; charset=US-ASCII > > Personally, I would not initiate any drastic changes at this point. > Keep in mind that these are guidelines; however, you must validate > your testing if you are going to follow other fixation guidelines. I > think everyone knowledgeable with this issue knows that the problem is > with underfixation, not overfixation. I recently pulled tumor out of > formalin after 8 months of fixation and the IHC was still "3+" and the > FISH showed beautiful amplification (ratio of 10.0). I hope that once > the scientific evidence is evaluated, these guidelines will be > changed. Major expense is being incurred here unnecessarily. How is > your concordance between IHC and FISH for the detection of HER2? If > it's not broken, don't try to fix it. Our "ad-hoc" committee on IHC > standardization is meeting in Santa Barbara on Sunday and I hope this > issue will be discussed. > > Richard > > Richard W. Cartun, Ph.D. > Director, Immunopathology & Histology > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. > Try it now. From kbradshaw <@t> lcpath.com Thu Jan 24 11:14:49 2008 From: kbradshaw <@t> lcpath.com (Kari Bradshaw) Date: Thu Jan 24 11:15:16 2008 Subject: [Histonet] Re: HER2 fixation time In-Reply-To: <325597.93538.qm@web61211.mail.yahoo.com> Message-ID: <391406d7b3921c489bf141c45ae5462f@mail2.lcpath.com> I agree fully. We have two VIP's. One is set Friday night to process overnight and the tissue sits in paraffin until Monday morning. The other processor is set Saturday morning to run an overnight process and those tissues sit in paraffin until Monday morning as well. Kari Bradshaw Anatomic Pathology Manager Lower Columbia Pathologists 1217 14th Ave Longview, WA 98632 360.425.5620 kbradshaw@lcpath.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, January 24, 2008 8:47 AM To: ancillarypath@mac.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: HER2 fixation time Not if you set the instrument to start EXACTLY when you want. You will have the tissues in melted paraffin more time, or you can extend the dehydration times. You can do a lot of things all preventing longer fixation in NBF. Modern TP are very flexible instruments and I have no data that reflects any deleterious effects for the tissues' reactive qualities or sectioning characteristics after being in melted paraffin for long periods of time. Ren? J. ancillarypath@mac.com wrote: I agree with Rich, and it's good to hear that some colleagues have started their own mode of cross-validation. If you choose to deviate from the upper fixation limit of 48 hours, you will ONLY be at default if you do not have evidence (with documentation) that raising the upper fixation limits to 72 or 96 hours has no detrimental effects on the results. The CAP will eventually increase the upper limit to 72 (or hopefully 96) hours once there is solid evidence that is ok to do so. Rene's suggestion to put the instrument on delay is not a valid solution. As long as the tissue is sitting in formalin in the instrument while it's on delay, it's still being fixed. This issue was discussed at the ASCO/CAP meeting. Hadi ============================== Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories, President, Ancillary Pathways 7000 SW 62nd Avenue, Suite Penthouse-C Miami, FL 33143 Tel 305.740.4440 Fax 786.513.0175 www.vitromolecular.com www.ancillarypath.com This email message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e- mail and destroy all copies of the original message. Message: 18 Date: Thu, 24 Jan 2008 09:57:05 -0500 From: "Richard Cartun" Subject: Re: [Histonet] HER2neu Fixation times To: "Ramona Turner" , Message-ID: <479860F2020000770000A527@gwmail4.harthosp.org> Content-Type: text/plain; charset=US-ASCII Personally, I would not initiate any drastic changes at this point. Keep in mind that these are guidelines; however, you must validate your testing if you are going to follow other fixation guidelines. I think everyone knowledgeable with this issue knows that the problem is with underfixation, not overfixation. I recently pulled tumor out of formalin after 8 months of fixation and the IHC was still "3+" and the FISH showed beautiful amplification (ratio of 10.0). I hope that once the scientific evidence is evaluated, these guidelines will be changed. Major expense is being incurred here unnecessarily. How is your concordance between IHC and FISH for the detection of HER2? If it's not broken, don't try to fix it. Our "ad-hoc" committee on IHC standardization is meeting in Santa Barbara on Sunday and I hope this issue will be discussed. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bwhitaker <@t> brownpathology.com Thu Jan 24 11:29:29 2008 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Thu Jan 24 11:25:13 2008 Subject: [Histonet] Re: HER2 fixation time In-Reply-To: <9C02888C-455A-448B-BF3F-6B722BBF871B@mac.com> Message-ID: <001c01c85eae$ad92ab10$3601a8c0@brownpathology.net> What about programming the processor to go 48 hours in formalin, then set the remainder of the time in 50 or 70% ETOH? It would require math skills and starting the processor at a precise time, rather than doing a delay start (at least with the processors I know about they don't have a 1st hold and 2nd hold ability) but would there be a problem with that from a regulatory perspective? Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ancillarypath@mac.com Sent: Thursday, January 24, 2008 10:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: HER2 fixation time I agree with Rich, and it's good to hear that some colleagues have started their own mode of cross-validation. If you choose to deviate from the upper fixation limit of 48 hours, you will ONLY be at default if you do not have evidence (with documentation) that raising the upper fixation limits to 72 or 96 hours has no detrimental effects on the results. The CAP will eventually increase the upper limit to 72 (or hopefully 96) hours once there is solid evidence that is ok to do so. Rene's suggestion to put the instrument on delay is not a valid solution. As long as the tissue is sitting in formalin in the instrument while it's on delay, it's still being fixed. This issue was discussed at the ASCO/CAP meeting. Hadi ============================== Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories, President, Ancillary Pathways 7000 SW 62nd Avenue, Suite Penthouse-C Miami, FL 33143 Tel 305.740.4440 Fax 786.513.0175 www.vitromolecular.com www.ancillarypath.com This email message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e- mail and destroy all copies of the original message. Message: 18 Date: Thu, 24 Jan 2008 09:57:05 -0500 From: "Richard Cartun" Subject: Re: [Histonet] HER2neu Fixation times To: "Ramona Turner" , Message-ID: <479860F2020000770000A527@gwmail4.harthosp.org> Content-Type: text/plain; charset=US-ASCII Personally, I would not initiate any drastic changes at this point. Keep in mind that these are guidelines; however, you must validate your testing if you are going to follow other fixation guidelines. I think everyone knowledgeable with this issue knows that the problem is with underfixation, not overfixation. I recently pulled tumor out of formalin after 8 months of fixation and the IHC was still "3+" and the FISH showed beautiful amplification (ratio of 10.0). I hope that once the scientific evidence is evaluated, these guidelines will be changed. Major expense is being incurred here unnecessarily. How is your concordance between IHC and FISH for the detection of HER2? If it's not broken, don't try to fix it. Our "ad-hoc" committee on IHC standardization is meeting in Santa Barbara on Sunday and I hope this issue will be discussed. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ancillarypath <@t> mac.com Thu Jan 24 11:26:34 2008 From: ancillarypath <@t> mac.com (Dr. Hadi Yaziji) Date: Thu Jan 24 11:26:59 2008 Subject: [Histonet] Re: HER2 fixation time In-Reply-To: <391406d7b3921c489bf141c45ae5462f@mail2.lcpath.com> References: <391406d7b3921c489bf141c45ae5462f@mail2.lcpath.com> Message-ID: <76C0D8B2-B483-44E9-8FDA-89A9F2CE400C@mac.com> Kari, Let me state this one more time: deviation from the standard range of exposure to each of the TP components needs to be validated. You have the responsibility to show that the level of signal remains the same compared to the standard time in melted paraffin. If you don't have documentation to this effect, then you are in violation of the guidelines. If your CAP inspector approved your method, it's because they are not adequately trained to evaluate your assay compliance. Hadi Yaziji On Jan 24, 2008, at 12:14 PM, Kari Bradshaw wrote: > I agree fully. We have two VIP's. One is set Friday night to process > overnight and the tissue sits in paraffin until Monday morning. The > other processor is set Saturday morning to run an overnight process > and > those tissues sit in paraffin until Monday morning as well. > > Kari Bradshaw > Anatomic Pathology Manager > Lower Columbia Pathologists > 1217 14th Ave > Longview, WA 98632 > 360.425.5620 > kbradshaw@lcpath.com > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J > Buesa > Sent: Thursday, January 24, 2008 8:47 AM > To: ancillarypath@mac.com; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Re: HER2 fixation time > > Not if you set the instrument to start EXACTLY when you want. You will > have the tissues in melted paraffin more time, or you can extend the > dehydration times. You can do a lot of things all preventing longer > fixation in NBF. Modern TP are very flexible instruments and I have no > data that reflects any deleterious effects for the tissues' reactive > qualities or sectioning characteristics after being in melted paraffin > for long periods of time. > Ren? J. > > ancillarypath@mac.com wrote: > I agree with Rich, and it's good to hear that some colleagues have > started their own mode of cross-validation. > > If you choose to deviate from the upper fixation limit of 48 hours, > you > will ONLY be at default if you do not have evidence (with > documentation) that raising the upper fixation limits to 72 or 96 > hours > has no detrimental effects on the results. The CAP will eventually > increase the upper limit to 72 (or hopefully 96) hours once there is > solid evidence that is ok to do so. > > Rene's suggestion to put the instrument on delay is not a valid > solution. As long as the tissue is sitting in formalin in the > instrument > while it's on delay, it's still being fixed. This issue was > discussed at > the ASCO/CAP meeting. > > Hadi > > ============================== > Hadi Yaziji, M.D., Medical Director > Vitro Molecular Laboratories, > President, Ancillary Pathways > 7000 SW 62nd Avenue, Suite Penthouse-C > Miami, FL 33143 > Tel 305.740.4440 > Fax 786.513.0175 > www.vitromolecular.com > www.ancillarypath.com > > This email message, including any attachments, is for the sole use of > the intended recipients and may contain privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. > If you are not the intended recipient, please contact the sender by e- > mail and destroy all copies of the original message. > > > Message: 18 > Date: Thu, 24 Jan 2008 09:57:05 -0500 > From: "Richard Cartun" > Subject: Re: [Histonet] HER2neu Fixation times > To: "Ramona Turner" , > > Message-ID: <479860F2020000770000A527@gwmail4.harthosp.org> > Content-Type: text/plain; charset=US-ASCII > > Personally, I would not initiate any drastic changes at this point. > Keep in mind that these are guidelines; however, you must validate > your > testing if you are going to follow other fixation guidelines. I think > everyone knowledgeable with this issue knows that the problem is with > underfixation, not overfixation. I recently pulled tumor out of > formalin > after 8 months of fixation and the IHC was still "3+" and the FISH > showed beautiful amplification (ratio of 10.0). I hope that once the > scientific evidence is evaluated, these guidelines will be changed. > Major expense is being incurred here unnecessarily. How is your > concordance between IHC and FISH for the detection of HER2? If it's > not > broken, don't try to fix it. Our "ad-hoc" committee on IHC > standardization is meeting in Santa Barbara on Sunday and I hope this > issue will be discussed. > > Richard > > Richard W. Cartun, Ph.D. > Director, Immunopathology & Histology > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. > Try > it now. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From jcline <@t> wchsys.org Thu Jan 24 12:02:55 2008 From: jcline <@t> wchsys.org (Joyce Cline) Date: Thu Jan 24 12:03:20 2008 Subject: [Histonet] Christi Smith/ slide racks In-Reply-To: <20080124103258.870gn83tfm9csocw@webmail.up2speedsolutions.com> Message-ID: <001101c85eb3$59c37dc0$1d2a14ac@wchsys.org> Sorry, I have already passed them on to someone. -----Original Message----- From: csmith@primepathlab.com [mailto:csmith@primepathlab.com] Sent: Thursday, January 24, 2008 12:33 PM To: jcline@wchsys.org Subject: slide racks I saw your advertisement for the Varistainer slide racks. Do you still have them? If you do how much are you asking. Please email me back with the information. Thank you, Christi Smith Prime Pathology ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From rjbuesa <@t> yahoo.com Thu Jan 24 12:12:06 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 24 12:12:27 2008 Subject: [Histonet] Re: HER2 fixation time In-Reply-To: <76C0D8B2-B483-44E9-8FDA-89A9F2CE400C@mac.com> Message-ID: <264667.52422.qm@web61219.mail.yahoo.com> Where can I find the CAP validation tests? What is the level of capriciousness in their protocol? Did they quantified or even tested the results for tissues fixed more than 48 hours? Is it that they just work Monday thru Fridays? Did they analyze the administrative and scheduling effects of their protocol? Do they care about that? Is this protocol the result of sound and comparative tests during different periods of time, or is just "bug brother" working as histotech now? Ren? J. "Dr. Hadi Yaziji" wrote: > > --------------------------------- Never miss a thing. Make Yahoo your homepage. From dont8know8me <@t> yahoo.com Thu Jan 24 12:27:34 2008 From: dont8know8me <@t> yahoo.com (richard peralta) Date: Thu Jan 24 12:27:57 2008 Subject: [Histonet] (no subject) Message-ID: <867644.18934.qm@web51406.mail.re2.yahoo.com> helloo all histonetters.....i need info about validation of antibodies if theres any please email me ..i really appreciate it....did CAP has any universal protocol for validating immuno antibodies?.......THANK YOU so much for ur info and ur help ____________________________________________________________________________________ Never miss a thing. Make Yahoo your home page. http://www.yahoo.com/r/hs From ancillarypath <@t> mac.com Thu Jan 24 12:33:08 2008 From: ancillarypath <@t> mac.com (Dr. Hadi Yaziji) Date: Thu Jan 24 12:33:35 2008 Subject: [Histonet] Re: HER2 fixation time In-Reply-To: <264667.52422.qm@web61219.mail.yahoo.com> References: <264667.52422.qm@web61219.mail.yahoo.com> Message-ID: Rene, Once you read the published article in the January issue of the Archives of Pathology and Lab Medicine (available on the CAP website to download for free), you will have a better idea about the extend of work the committee has gone through to set the parameters. Their work is *outstanding*, but it's not perfect, but the outcome is safe and badly needed. Your criticism is well taken, but it's not necessary. Hadi On Jan 24, 2008, at 1:12 PM, Rene J Buesa wrote: > Where can I find the CAP validation tests? What is the level of > capriciousness in their protocol? > Did they quantified or even tested the results for tissues fixed > more than 48 hours? Is it that they just work Monday thru Fridays? > Did they analyze the administrative and scheduling effects of their > protocol? Do they care about that? > Is this protocol the result of sound and comparative tests during > different periods of time, or is just "bug brother" working as > histotech now? > Ren? J. > > "Dr. Hadi Yaziji" wrote: > > > > > > > > > Never miss a thing. Make Yahoo your homepage. From Cathy.Crumpton <@t> tuality.org Thu Jan 24 12:49:49 2008 From: Cathy.Crumpton <@t> tuality.org (Cathy.Crumpton@tuality.org) Date: Thu Jan 24 12:50:13 2008 Subject: [Histonet] I need some human fetal liver Message-ID: Hello all, I am i Would an hospital and we From AGrobe2555 <@t> aol.com Thu Jan 24 12:49:44 2008 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Thu Jan 24 12:50:16 2008 Subject: [Histonet] Ki67 in sheep Message-ID: Hello all, Can anyone recommend an antibody and staining protocol for Ki-67 that can be used in FFPE sheep tissues. Normal tissue(s) to be used as a control would also be helpful (we don't have access to anything but normal tissue). We are currently trying to optimize the antibody we have with varying results.... Thanks, Albert Albert C. Grobe, PhD Tissue Engineering Lab International Heart Institute of Montana Foundation **************Start the year off right. Easy ways to stay in shape. http://body.aol.com/fitness/winter-exercise?NCID=aolcmp00300000002489 From liz <@t> premierlab.com Thu Jan 24 13:05:13 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Jan 24 13:05:42 2008 Subject: [Histonet] Ki67 in sheep In-Reply-To: <3DA551AE8CD74596841B6FD3A17ECB02@PremierLab.local> References: <3DA551AE8CD74596841B6FD3A17ECB02@PremierLab.local> Message-ID: Albert I have not tried this specific antibody in sheep, but we have used it in mouse, rat, human, canine tissues, and possibly porcine, but I can't remember off hand. Its from Santa Cruz sc-15402 H-300 Rabbit polyclonal, it might work in sheep. I have a protocol if needed. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of AGrobe2555@aol.com Sent: Thursday, January 24, 2008 12:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ki67 in sheep Hello all, Can anyone recommend an antibody and staining protocol for Ki-67 that can be used in FFPE sheep tissues. Normal tissue(s) to be used as a control would also be helpful (we don't have access to anything but normal tissue). We are currently trying to optimize the antibody we have with varying results.... Thanks, Albert Albert C. Grobe, PhD Tissue Engineering Lab International Heart Institute of Montana Foundation **************Start the year off right. Easy ways to stay in shape. http://body.aol.com/fitness/winter-exercise?NCID=aolcmp00300000002489 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rcharles <@t> state.pa.us Thu Jan 24 13:14:11 2008 From: rcharles <@t> state.pa.us (Charles, Roger) Date: Thu Jan 24 13:14:59 2008 Subject: [Histonet] Sakura Slide writer Message-ID: <12E4E17FEF6EBE4BAE95BEB3CDCB57000943BB59@enhbgpri04.backup> Hello all, Is anybody using the Sakura slide writer that can give me their opinion on this instrument? I would like to know pros and cons and if you are using charged slides and having problems related to the charge. Also I would like an opinion on the software package that is used by the printer. Thanks Roger Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 From GDawson <@t> dynacaremilwaukee.com Thu Jan 24 13:26:16 2008 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Thu Jan 24 13:26:43 2008 Subject: [Histonet] Re: HER2 fixation time In-Reply-To: Message-ID: All, Once we all agree to fix our tissues for EXACTLY the same amount of time in the same fixative (supplied by the same vendor of course), what do we do with all of the other factors involved to reach this pipe dream of national standardization? The next step must be a requirement for all of us to work in the same hermetically sealed, mass produced "pre-fab" labs that can all provide identical water sources, temperatures, humidity, elevation/pressure, etc... We must all agree to use identical antigen retrieval methods, kits/detections, chromagens, etc... provided by the same "super vendor" with the same lot numbers. This may be feasable with all the buyouts in our industry but remains highly unlikely. Lastly, we must all agree to invite in only the same gremlins since they seem to thrive in so many laboratory environments. My point is, the new CAP HER2 guidlines are a fruitless attempt to standardize a procedure that has too many factors involved to achieve true, nation-wide standardization. The current discussion on the fixation aspect needs to be tempered by the fact that it is still only one factor in many. Does anyone else get that sick feeling that this is just the tip of the iceburg and calls for pre-fab kits and national standardization for many other tests are just around the corner? Things that make you go HMMMMMM, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI From kbradshaw <@t> lcpath.com Thu Jan 24 13:32:34 2008 From: kbradshaw <@t> lcpath.com (Kari Bradshaw) Date: Thu Jan 24 13:33:01 2008 Subject: [Histonet] Re: HER2 fixation time In-Reply-To: Message-ID: <8bdb1ff78104814cb1a90473bc37ea0e@mail2.lcpath.com> Go Glen, very well put! Kari Bradshaw Anatomic Pathology Manager Lower Columbia Pathologists 1217 14th Ave Longview, WA 98632 360.425.5620 kbradshaw@lcpath.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Thursday, January 24, 2008 11:26 AM Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: HER2 fixation time All, Once we all agree to fix our tissues for EXACTLY the same amount of time in the same fixative (supplied by the same vendor of course), what do we do with all of the other factors involved to reach this pipe dream of national standardization? The next step must be a requirement for all of us to work in the same hermetically sealed, mass produced "pre-fab" labs that can all provide identical water sources, temperatures, humidity, elevation/pressure, etc... We must all agree to use identical antigen retrieval methods, kits/detections, chromagens, etc... provided by the same "super vendor" with the same lot numbers. This may be feasable with all the buyouts in our industry but remains highly unlikely. Lastly, we must all agree to invite in only the same gremlins since they seem to thrive in so many laboratory environments. My point is, the new CAP HER2 guidlines are a fruitless attempt to standardize a procedure that has too many factors involved to achieve true, nation-wide standardization. The current discussion on the fixation aspect needs to be tempered by the fact that it is still only one factor in many. Does anyone else get that sick feeling that this is just the tip of the iceburg and calls for pre-fab kits and national standardization for many other tests are just around the corner? Things that make you go HMMMMMM, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Jan 24 13:37:14 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 24 13:37:37 2008 Subject: [Histonet] Re: HER2 fixation time In-Reply-To: Message-ID: <62789.3461.qm@web61217.mail.yahoo.com> Is now "Big brother" in the histology business? All this standardization to get to a QUALITATIVE result? Where are the "standard" photomicrographs printed identically with the same standard color quality prints to compare with? I know of two color blind pathologists and three with poor vision. Ren? J. "Dawson, Glen" wrote: All, Once we all agree to fix our tissues for EXACTLY the same amount of time in the same fixative (supplied by the same vendor of course), what do we do with all of the other factors involved to reach this pipe dream of national standardization? The next step must be a requirement for all of us to work in the same hermetically sealed, mass produced "pre-fab" labs that can all provide identical water sources, temperatures, humidity, elevation/pressure, etc... We must all agree to use identical antigen retrieval methods, kits/detections, chromagens, etc... provided by the same "super vendor" with the same lot numbers. This may be feasable with all the buyouts in our industry but remains highly unlikely. Lastly, we must all agree to invite in only the same gremlins since they seem to thrive in so many laboratory environments. My point is, the new CAP HER2 guidlines are a fruitless attempt to standardize a procedure that has too many factors involved to achieve true, nation-wide standardization. The current discussion on the fixation aspect needs to be tempered by the fact that it is still only one factor in many. Does anyone else get that sick feeling that this is just the tip of the iceburg and calls for pre-fab kits and national standardization for many other tests are just around the corner? Things that make you go HMMMMMM, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From eca9 <@t> georgetown.edu Thu Jan 24 14:12:20 2008 From: eca9 <@t> georgetown.edu (Eva CA Permaul) Date: Thu Jan 24 14:14:01 2008 Subject: [Histonet] mouse spinal cord processing???? Message-ID: <1de4ae1e1b19.1e1b191de4ae@imap.georgetown.edu> Good afternoon everyone, We just had one of our investigators bring down mouse spinal cord for processing. Could someone tell me what protocol we should use for this tissue? Such as how long to process it for and is there something specific we should look out for? Never done this before. Thank you for all your advice in advance, Eva Georgetown University From andrew.wang <@t> tufts.edu Thu Jan 24 14:31:56 2008 From: andrew.wang <@t> tufts.edu (Andrew Wang) Date: Thu Jan 24 14:32:17 2008 Subject: [Histonet] Basic Histology Questions Message-ID: <6c0279d90801241231g39b238c3m3597b2ffd7634ea9@mail.gmail.com> Hi, I am trying to stain IL-17 in mouse tissue. I am having difficulty and was wondering if anyone could guide me. Also, I am lacking basic histology knowledge and was hoping someone could clarify some issues for me. 1. PBS vs TBS for wash (adding tween?) 2. PFA vs Formalin vs. Methanol vs. Acetone to fix 3. Saponin vs Triton for permeabilization? 4. Antigen unmasking: pepsin, citrate buffer? I've been having trouble finding any information that would suggest a specific reason for choosing any one of those variables, but i'm sure there must be something out there? Is there any logic behind any of this "hoo ha?" Ready to give up, Andrew Wang MSIV Department of Pathology Tufts University Sackler School of Graduate Biomedical Sciences University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School From elockman <@t> apsemail.com Thu Jan 24 15:21:48 2008 From: elockman <@t> apsemail.com (Emily Lockman) Date: Thu Jan 24 15:22:00 2008 Subject: [Histonet] Employment Opportunity in MN Message-ID: <037BDA8D37760D49A2A23D1C877EA8C945D14A@apsdc01.aps.dom> American Preclinical Services, LLC is a private Medical Device Contract Research Organization (CRO) looking for a full-time Histopathology Technician. Duties to include histology processes including paraffin and plastic sectioning. Anatomic Pathology duties including performing necropsies, documenting findings, trimming tissue and histological processing. Other duties may include collection, handling and shipping of clinical pathology samples (blood, urine, feces), animal care, surgical assistance and facility maintenance. Shift is 10:00-6:30PM We offer excellent benefits including a competitive salary, medical, dental, short and long-term disability and 401K. We operate on a Paid Time Off (PTO) system to allow for vacation flexibility. Qualifications: BS or AS degree and graduation from an approved Histotechnology program or equivalent. HTL or HT (ASCP) registration. Excellent communication skills (written and verbal) required. Experience in plastics is a plus, however the aptitude to learn new techniques is more important. If interested or want more information, please contact Jason Thorsten (jthorsten@apsemail.com or (763) 717- 7990 ext. 2003). Emily M. Lockman, HT (ASCP) Histotechnologist II, Pathology Services American Preclinical Services, LLC (APS) 8945 Evergreen Boulevard Minneapolis, MN 55433 Phone: 763-717-7990 ext. 2025 (necropsy) ext. 2006 (voicemail) Fax: 763-717-2042 elockman@apsemail.com From Djemge <@t> aol.com Thu Jan 24 16:42:41 2008 From: Djemge <@t> aol.com (Djemge@aol.com) Date: Thu Jan 24 16:42:57 2008 Subject: [Histonet] Re: Olympus BH-2 microscopy Message-ID: Re: Olympus BH-2 microscopy Patty, Yahoo has a Microscope list group at _http://tech.groups.yahoo.com/group/Microscope/_ (http://tech.groups.yahoo.com/group/Microscope/) that has been fantastic for helping me to find microscope parts and manuals. It?s kind of the histonet for microscopy ? they share tips on equipment, supplies and procedures. I bet they would know just where to get the part you need for your Olympus BH-2. Good luck. Donna J. Emge, HT-ASCP Northwestern University Feinberg School of Medicine 303 East Superior St, Lurie 7-220 Chicago, IL 60611 312-503-2036 _d-emge@northwestern.edu_ (mailto:d-emge@northwestern.edu) **************Biggest Grammy Award surprises of all time on AOL Music. (http://music.aol.com/grammys/pictures/never-won-a-grammy?NCID=aolcmp003000000025 48) From jacobc <@t> mmc.org Thu Jan 24 19:17:09 2008 From: jacobc <@t> mmc.org (Christine Jacobs) Date: Thu Jan 24 19:17:42 2008 Subject: [Histonet] PMS2 Message-ID: <20080124T201709Z_C07000110000@mmc.org> Fellow Histonetters! I have seen a few threads lately about PMS-2 antibody. I am also trying to get this one on line and really don't want to bring on an RUO-rated antibody. However, that is all that is currently available as far as I can tell. I just spoke to the representative from Cell Marque. They will be launching their version of PMS-2 later this year. As of today's conversation, the rep. was uncertain of what the rating will be but it will be interesting to watch this one to see how they rate it. Cell Marque seems to dislike RUO's as much as I do so there is potential here for something with at least an ASR rating! Just thought I'd share the info I was given so any of you looking for this antibody can keep your eyes on this one! Chris Jacobs, HT(ASCP), QIHC NorDx Laboratory Scarborough, ME jacobc@mmc.org CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the use of the intended recipient(s) only and may contain information that is privileged, confidential, and prohibited from unauthorized disclosure under applicable law. If you are not the intended recipient of this message, any dissemination, distribution, or copying of this message is strictly prohibited. If you received this message in error, please notify the sender by reply email and destroy all copies of the original message and attachments. From pdunlop720 <@t> gmail.com Thu Jan 24 19:59:06 2008 From: pdunlop720 <@t> gmail.com (Patty Dunlop) Date: Thu Jan 24 19:59:17 2008 Subject: [Histonet] if you could choose any microtome... Message-ID: <80ab7bc60801241759k74f2f4b1gab17aab7ad2f26f6@mail.gmail.com> Hello, If you had the opportunity to order any microtome of your choice, which one would you order and why? Is there a better brand in terms of equipment or service? The only one I have ever used is a Microm motorized. I did like that one, but I have nothing to compare it to. Thanks, Patty From koellingr <@t> comcast.net Thu Jan 24 22:48:25 2008 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Thu Jan 24 22:48:39 2008 Subject: [Histonet] CD25 - Rat Message-ID: <012520080448.9124.47996A1700062E01000023A422007637049D09020704040A0105@comcast.net> Joost, mainly mouse but some rat work with CD25. CD25 is the low affinity IL-2R alpha. So found on T-cells that are activated a lot. But as with a lot of these markers you can find increased expression in activating circumstances and find CD25 on macrophages and dendritic cells also. But shouldn't be as much as you seem to describe. PALS I agree, less in B-cell area, marginal zone macs maybe OK. Even red pulp lymphocytes ok since there is constitutive expression on Treg cells. But capillaries, all over? Something is wrong there if this is just a normal, adult rat that hasn't had something like a sham injection of some protein or immunoglobulin. What is your detection? If possible, let someone flow rat lymphoid tissue (same strain) and for them it would be a snap to do 2-color flow for CD3/CD25, mac marker/CD25, etc and you will get a feel of how many kinds of cells and percentages should be staining in your tissue. Remembering back to my projects wouldn't help you since your str ains and IHC detection are probably much different. But if this is a normal, control rat, sounds like a bit too much staining to me. Ray Koelling PhenoPath Labs Seattle, WA -------------- Original message -------------- From: "Bruijntjes, J.P. (Joost)" > Hi all > > > > Is anyone of you familiar with staining rat lymphoid tissues with an > antibody directed against CD25? > > In the white pulp there is a lot of positive staining on different > lymphocytes in the PALS, (less in the) B-cell area. In the marginal zone > positive staining is seen on some large cells, most probably > macrophages. > > In the red pulp there is a lot of staining on different type of > lymphocytes too. But I see a lot of positive staining on (most probably) > capillaries (endothelial cells) . Not a few capillaries, but all. > > Does it sound familiar to anyone of you? > > > > Joost Bruijntjes > > TNO Quality of Life > > Zeist > > The Netherlands > > > > > > TNO.NL > > Joost Bruijntjes > > T +31 30 694 44 80 > F +31 30 694 49 86 > E joost.bruijntjes@tno.nl > > Disclaimer > > > > > This e-mail and its contents are subject to the DISCLAIMER at > http://www.tno.nl/disclaimer/email.html > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Thu Jan 24 23:18:59 2008 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Thu Jan 24 23:13:05 2008 Subject: [Histonet] Decal Bone Antigen retrieval In-Reply-To: Message-ID: <200801250512.m0P5Clsq039811@pro12.abac.com> I avoid hier and especially in a pressure cooker for bone IHC. Like Liz I prefer enzyme digestion to hier for bone, if I have too, I use a gentle steamer or waterbath and not a pressure cooker. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Tuesday, January 22, 2008 12:18 PM To: Igor Deyneko; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Decal Bone Antigen retrieval Igor Its going to depend upon what antibody you are trying to detect but if possible I would try enzyme retreival over HIER, my favorite is proteinase K, but we also use pepsin, pronase, chondroitinase, etc. It depends upon the antibody. If you have to use HIER you can its just thats it likely you are going to loose portions of the tissue. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Igor Deyneko Sent: Tuesday, January 22, 2008 12:01 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Decal Bone Antigen retrieval Dear histonetters! I have a question. Recently I have received decaled mouse hind paw joint slides, which I have to do IHC on. Can anyone advise an antigen retrieval solution and a method. The current method I use and has been working fine in the past is as follows: Either Citrate or DAKO Retrieval solution in a conventional pressure cooker for 20 minutes , then I would let it stand with the open lid for another 10 minutes, and finally would take the Coplin jars out and let them stand for an additional 30 mins at RT. Worked fine for tumors. Any ideas for joints??? All suggestions would be greatly appreciated. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA 02139 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Fri Jan 25 04:53:26 2008 From: louise.renton <@t> gmail.com (louise renton) Date: Fri Jan 25 04:53:42 2008 Subject: [Histonet] $ rate for immunos Message-ID: Hi all, and a happy friday to you too! This has been asked me by a friend and I wasn't sure how to answer, so i am consulting th egreat Histo oracle..... If one is doing contract work, how do you calculate what to charge for doing immunos? per slide, per hour? (materials supplied by researcher, so no charge there for consumables) If there is optimization involved?? Lokking forward both to hearing from y'all and to the weekend! -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From mareike <@t> hi.is Fri Jan 25 05:39:00 2008 From: mareike <@t> hi.is (Mareike Heimann) Date: Fri Jan 25 05:38:54 2008 Subject: [Histonet] O.C.T. embedding vs. freezing of unembedded samples Message-ID: <003401c85f46$e11b20a0$5a96d082@MrDarcy> Hi there, I have been wondering about the advantages (or disadvantages) of embedding tissue in O.C.T. before snap-freezing in liquid nitrogen when compared to snap-freezing the "naked" tissue. I do use O.C.T. before freezing, but when recently asked why, I found no better answer than "it seems to be what most people do..." I would be most grateful for any explanations! (My tissue blocks are used for immunohistochemical stainings later on) Regards, Mareike From jnocito <@t> satx.rr.com Fri Jan 25 06:02:22 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Jan 25 06:02:38 2008 Subject: [Histonet] Re: HER2 fixation time References: <325597.93538.qm@web61211.mail.yahoo.com> <50F106F7-994E-4050-9511-DDC8A0743DA1@mac.com> Message-ID: <004701c85f4a$2535ca80$0302a8c0@yourxhtr8hvc4p> Was it CAP who came up with these guidelines, I forget (everyone does say that the mind is the first to go)? Did anyone have scientific proof about the 72 hour limit, or was this an arbitrary number that CAP came up with? It seems everyone across the planet is jumping through hoops for this one question. Correct me if I'm wrong, but is the standard that if Her2/neu is negative by immuno, it gets reflexed to FISH? I know FISH is more expensive, but if you keep getting correlating responses, wouldn't that be enough proof to shut up CAP? It is Friday and I haven't been flamed in a while. I guess this is why I never passed the rank of E-6 while I was in the Air Force. I could never keep my mouth shut over something like this. JTT ----- Original Message ----- From: "Dr. Hadi Yaziji" To: "Rene J Buesa" Cc: Sent: Thursday, January 24, 2008 11:07 AM Subject: Re: [Histonet] Re: HER2 fixation time This is a very good point, Rene. However, ... Prolonged exposure to melted paraffin (in 60 degrees) may be as bad (or most likely worse) than overfixing in formalin. Each time a standard parameter is drastically changed (such as over- exposure to dehydration or melted paraffin), that component NEEDS to be validated when it comes to predictive markers assay. I'd much rather validate 72-hr fixation than having to validate prolonged exposure to components of the tissue processor. Immunohistochemistry of predictive markers needs to be treated differently than diagnostic markers. It should be done with maximum care so we can tell the oncology community that the results our labs are producing are reliable and trustworthy. This is the essence of HER2 guidelines. The minute we start deviating from a pre-analytical parameter, the minute we start getting into trouble. In fact, if people have been fixing their speciment according to the FDA-approved fixation range of 6-24 hours, we wouldn't have been in the mess we're in right now. Hadi On Jan 24, 2008, at 11:46 AM, Rene J Buesa wrote: > Not if you set the instrument to start EXACTLY when you want. You will > have the tissues in melted paraffin more time, or you can extend the > dehydration times. You can do a lot of things all preventing longer > fixation in NBF. Modern TP are very flexible instruments and I have no > data that reflects any deleterious effects for the tissues' reactive > qualities or sectioning characteristics after being in melted paraffin > for long periods of time. > Ren? J. > > ancillarypath@mac.com wrote: > I agree with Rich, and it's good to hear that some colleagues have > started their own mode of cross-validation. > > If you choose to deviate from the upper fixation limit of 48 hours, > you will ONLY be at default if you do not have evidence (with > documentation) that raising the upper fixation limits to 72 or 96 > hours has no detrimental effects on the results. The CAP will > eventually increase the upper limit to 72 (or hopefully 96) hours once > there is solid evidence that is ok to do so. > > Rene's suggestion to put the instrument on delay is not a valid > solution. As long as the tissue is sitting in formalin in the > instrument while it's on delay, it's still being fixed. This issue was > discussed at the ASCO/CAP meeting. > > Hadi > > ============================== > Hadi Yaziji, M.D., Medical Director > Vitro Molecular Laboratories, > President, Ancillary Pathways > 7000 SW 62nd Avenue, Suite Penthouse-C > Miami, FL 33143 > Tel 305.740.4440 > Fax 786.513.0175 > www.vitromolecular.com > www.ancillarypath.com > > This email message, including any attachments, is for the sole use of > the intended recipients and may contain privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. > If you are not the intended recipient, please contact the sender by e- > mail and destroy all copies of the original message. > > > Message: 18 > Date: Thu, 24 Jan 2008 09:57:05 -0500 > From: "Richard Cartun" > Subject: Re: [Histonet] HER2neu Fixation times > To: "Ramona Turner" , > > Message-ID: <479860F2020000770000A527@gwmail4.harthosp.org> > Content-Type: text/plain; charset=US-ASCII > > Personally, I would not initiate any drastic changes at this point. > Keep in mind that these are guidelines; however, you must validate > your testing if you are going to follow other fixation guidelines. I > think everyone knowledgeable with this issue knows that the problem is > with underfixation, not overfixation. I recently pulled tumor out of > formalin after 8 months of fixation and the IHC was still "3+" and the > FISH showed beautiful amplification (ratio of 10.0). I hope that once > the scientific evidence is evaluated, these guidelines will be > changed. Major expense is being incurred here unnecessarily. How is > your concordance between IHC and FISH for the detection of HER2? If > it's not broken, don't try to fix it. Our "ad-hoc" committee on IHC > standardization is meeting in Santa Barbara on Sunday and I hope this > issue will be discussed. > > Richard > > Richard W. Cartun, Ph.D. > Director, Immunopathology & Histology > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it > now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Walzer <@t> HCAHealthcare.com Fri Jan 25 06:43:11 2008 From: Susan.Walzer <@t> HCAHealthcare.com (Walzer Susan) Date: Fri Jan 25 06:43:24 2008 Subject: [Histonet] HER2neu Fixation times In-Reply-To: References: Message-ID: <471953BC63077941B82C26A4338272B42F056F@ORLEV03.hca.corpad.net> We do not work weekends either but contacted some people and they told us we could do a Sat. morning run and let them remain in the wax til Mon. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ramona Turner Sent: Thursday, January 24, 2008 9:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HER2neu Fixation times This is a followup to a recent question asking what do small histology labs do about proper fixation time for HER2neu if there is no weekend rotation...... I would also like to know....other than having a rotation tech to start the processor on Saturday, I can't think of a solution....A Saturday rotation would be a great hardship for three techs to maintain, plus that is one of the perks of the job is not having to work weekends. Any ideas? Ramona Turner, HT (ASCP) Histology Supervisor POTOMAC HOSPITAL 2300 Opitz Blvd Woodbridge, VA 22191 _________________________________________________________________ Helping your favorite cause is as easy as instant messaging.?You IM, we give. http://im.live.com/Messenger/IM/Home/?source=text_hotmail_join_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histology <@t> gradymem.org Fri Jan 25 08:10:10 2008 From: histology <@t> gradymem.org (histology@gradymem.org) Date: Fri Jan 25 08:10:24 2008 Subject: [Histonet] if you could choose any microtome... In-Reply-To: <80ab7bc60801241759k74f2f4b1gab17aab7ad2f26f6@mail.gmail.com> References: <80ab7bc60801241759k74f2f4b1gab17aab7ad2f26f6@mail.gmail.com> Message-ID: I would buy a new Leica. Have been using one for years. Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org ----- Original Message ----- From: Patty Dunlop Date: Thursday, January 24, 2008 8:00 pm Subject: [Histonet] if you could choose any microtome... To: histonet@lists.utsouthwestern.edu > Hello, > > If you had the opportunity to order any microtome of your choice, > which one > would you order and why? Is there a better brand in terms of > equipment or > service? The only one I have ever used is a Microm motorized. I > did like > that one, but I have nothing to compare it to. > > Thanks, > Patty > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From 1dpeterson <@t> meriter.com Fri Jan 25 08:12:34 2008 From: 1dpeterson <@t> meriter.com (Peterson, Dan) Date: Fri Jan 25 08:12:46 2008 Subject: [Histonet] Lab remodel Message-ID: <328CBAE62F31C642B422970E879DFADC01A80272@pcwex01> I'm looking for likes and dislikes from those out there in Histoland that have been fortunate enough to update their work environment. Specifically I'm looking at work station configurations. Has anyone gone to u-shaped stations, and if so, how do the techs like them? I'm planning on at least 4' x 2.5' per station, counters 30" high. I'd like to make these as ergonomically friendly as possible. If anyone has blueprints that they would like to share, I'd love to see them. Thanks in advance! Dan Daniel R Peterson HT(ASCP) Histopathology Section Head Meriter Laboratories (608)-417-6557 1dpeterson@meriter.com CONFIDENTIALITY NOTICE: This message (including any attachments) is intended for the sole use of the individual and entity to whom it is addressed. This message may contain information that is confidential and is protected by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply email and then delete the message. Thank you. From talulahgosh <@t> gmail.com Fri Jan 25 08:14:44 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Jan 25 08:14:59 2008 Subject: [Histonet] if you could choose any microtome... In-Reply-To: References: <80ab7bc60801241759k74f2f4b1gab17aab7ad2f26f6@mail.gmail.com> Message-ID: Definitely Leica, their microtomes are top notch, along with accessories. We've never used any other microtome. Emily -- "Prosperity ripened the principle of decay...and, as soon as time or accident and removed the artificial supports, the stupendous fabric yielded to the pressure of its own weight...instead of inquiring why the Roman Empire was destroyed we should rather be surprised that it has subsisted for so long" -Edward Gibbon, Decline and Fall of the Roman Empire From rjbuesa <@t> yahoo.com Fri Jan 25 08:23:41 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 25 08:23:52 2008 Subject: [Histonet] if you could choose any microtome... In-Reply-To: <80ab7bc60801241759k74f2f4b1gab17aab7ad2f26f6@mail.gmail.com> Message-ID: <264505.66190.qm@web61214.mail.yahoo.com> Switch to Leica. Ren? J. Patty Dunlop wrote: Hello, If you had the opportunity to order any microtome of your choice, which one would you order and why? Is there a better brand in terms of equipment or service? The only one I have ever used is a Microm motorized. I did like that one, but I have nothing to compare it to. Thanks, Patty _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From rjbuesa <@t> yahoo.com Fri Jan 25 08:26:22 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 25 08:26:35 2008 Subject: [Histonet] $ rate for immunos In-Reply-To: Message-ID: <872893.54087.qm@web61223.mail.yahoo.com> I would charge by slide. If there is optimization involved also by slide in the optimization test. Ren? J. louise renton wrote: Hi all, and a happy friday to you too! This has been asked me by a friend and I wasn't sure how to answer, so i am consulting th egreat Histo oracle..... If one is doing contract work, how do you calculate what to charge for doing immunos? per slide, per hour? (materials supplied by researcher, so no charge there for consumables) If there is optimization involved?? Lokking forward both to hearing from y'all and to the weekend! -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From jqb7 <@t> CDC.GOV Fri Jan 25 08:27:54 2008 From: jqb7 <@t> CDC.GOV (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri Jan 25 08:28:20 2008 Subject: [Histonet] if you could choose any microtome... In-Reply-To: <264505.66190.qm@web61214.mail.yahoo.com> References: <80ab7bc60801241759k74f2f4b1gab17aab7ad2f26f6@mail.gmail.com> <264505.66190.qm@web61214.mail.yahoo.com> Message-ID: <34BB307EFC9A65429BBB49E330675F72045E242D@LTA3VS003.ees.hhs.gov> I love my Microm HM 360! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, January 25, 2008 9:24 AM To: Patty Dunlop; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] if you could choose any microtome... Switch to Leica. Ren? J. Patty Dunlop wrote: Hello, If you had the opportunity to order any microtome of your choice, which one would you order and why? Is there a better brand in terms of equipment or service? The only one I have ever used is a Microm motorized. I did like that one, but I have nothing to compare it to. Thanks, Patty _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Jan 25 08:31:31 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 25 08:31:46 2008 Subject: [Histonet] Lab remodel In-Reply-To: <328CBAE62F31C642B422970E879DFADC01A80272@pcwex01> Message-ID: <43788.69856.qm@web61214.mail.yahoo.com> Although "theoretical" advantageous, "U" shaped work stations are not that ergonomically adequate (frequent body twitching) and some HTs claim they feel like "caged-boxed". Ren? J. "Peterson, Dan" <1dpeterson@meriter.com> wrote: I'm looking for likes and dislikes from those out there in Histoland that have been fortunate enough to update their work environment. Specifically I'm looking at work station configurations. Has anyone gone to u-shaped stations, and if so, how do the techs like them? I'm planning on at least 4' x 2.5' per station, counters 30" high. I'd like to make these as ergonomically friendly as possible. If anyone has blueprints that they would like to share, I'd love to see them. Thanks in advance! Dan Daniel R Peterson HT(ASCP) Histopathology Section Head Meriter Laboratories (608)-417-6557 1dpeterson@meriter.com CONFIDENTIALITY NOTICE: This message (including any attachments) is intended for the sole use of the individual and entity to whom it is addressed. This message may contain information that is confidential and is protected by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply email and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From lblazek <@t> digestivespecialists.com Fri Jan 25 08:40:24 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Jan 25 08:38:56 2008 Subject: [Histonet] if you could choose any microtome... In-Reply-To: <34BB307EFC9A65429BBB49E330675F72045E242D@LTA3VS003.ees.hhs.gov> References: <80ab7bc60801241759k74f2f4b1gab17aab7ad2f26f6@mail.gmail.com><264505.66190.qm@web61214.mail.yahoo.com> <34BB307EFC9A65429BBB49E330675F72045E242D@LTA3VS003.ees.hhs.gov> Message-ID: <1F937FB30BDB7C4A9F39F83FEA8D379F3E4EF6@bruexchange1.digestivespecialists.com> I love both my Microms. TGIF! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Friday, January 25, 2008 9:28 AM To: Rene J Buesa; Patty Dunlop; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] if you could choose any microtome... I love my Microm HM 360! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, January 25, 2008 9:24 AM To: Patty Dunlop; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] if you could choose any microtome... Switch to Leica. Ren? J. Patty Dunlop wrote: Hello, If you had the opportunity to order any microtome of your choice, which one would you order and why? Is there a better brand in terms of equipment or service? The only one I have ever used is a Microm motorized. I did like that one, but I have nothing to compare it to. Thanks, Patty _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmahoney <@t> alegent.org Fri Jan 25 08:43:32 2008 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Fri Jan 25 08:44:34 2008 Subject: [Histonet] if you could choose any microtome... In-Reply-To: <264505.66190.qm@web61214.mail.yahoo.com> References: <80ab7bc60801241759k74f2f4b1gab17aab7ad2f26f6@mail.gmail.com> <264505.66190.qm@web61214.mail.yahoo.com> Message-ID: <4799A1340200003C00028FB5@gwia.alegent.org> I agree completely. Jan Mahoney >>> Rene J Buesa 01/25/2008 8:23 AM >>> Switch to Leica. Ren? J. Patty Dunlop wrote: Hello, If you had the opportunity to order any microtome of your choice, which one would you order and why? Is there a better brand in terms of equipment or service? The only one I have ever used is a Microm motorized. I did like that one, but I have nothing to compare it to. Thanks, Patty _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Weber2 <@t> va.gov Fri Jan 25 08:53:04 2008 From: Susan.Weber2 <@t> va.gov (Weber, Susan (VHACLE)) Date: Fri Jan 25 08:53:15 2008 Subject: [Histonet] Lab Redesign Message-ID: <16C83872A53F4346AA9C3A18E3A3AAB902276CCB@VHAV10MSGA1.v10.med.va.gov> Hello all, and Happy Friday! We are also currently involved in a lab redesign which includes Histology, Cytology (as a separate department), EM (also separate) and the Morgue. We have just begun to define the "space" with the architects and welcome any suggestions from the Histo Guru's out there! We are also redesigning the clinical side of the lab as well. If you are around/near the Cleveland, Ohio area and have recently redesigned the lab clinical or anatomic, I would be especially interested to hear from you. You can e-mail me direct or on the "net". Thanks, and have a nice weekend! :-) Susan M. Weber, HT(ASCP) Louis Stokes Cleveland VA Medical Center Supervisor, Histology Laboratory (216) 791-3800 ext 6154 From slappycraw <@t> yahoo.com Fri Jan 25 08:53:29 2008 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Fri Jan 25 08:53:42 2008 Subject: [Histonet] if you could choose any microtome... Message-ID: <370312.18435.qm@web53611.mail.re2.yahoo.com> The Microm HM 360 is a thing of beauty! But I like the Leica as well so get both. Larry A. Woody Seattle, Wa. ----- Original Message ---- From: "Blazek, Linda" To: histonet@lists.utsouthwestern.edu Sent: Friday, January 25, 2008 6:40:24 AM Subject: RE: [Histonet] if you could choose any microtome... I love both my Microms. TGIF! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Friday, January 25, 2008 9:28 AM To: Rene J Buesa; Patty Dunlop; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] if you could choose any microtome... I love my Microm HM 360! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, January 25, 2008 9:24 AM To: Patty Dunlop; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] if you could choose any microtome... Switch to Leica. Ren? J. Patty Dunlop wrote: Hello, If you had the opportunity to order any microtome of your choice, which one would you order and why? Is there a better brand in terms of equipment or service? The only one I have ever used is a Microm motorized. I did like that one, but I have nothing to compare it to. Thanks, Patty _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Never miss a thing. Make Yahoo your home page. http://www.yahoo.com/r/hs From cmiller <@t> physlab.com Fri Jan 25 09:01:21 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Fri Jan 25 09:01:01 2008 Subject: [Histonet] if you could choose any microtome... In-Reply-To: References: <80ab7bc60801241759k74f2f4b1gab17aab7ad2f26f6@mail.gmail.com> Message-ID: <001201c85f63$25b26b30$3d02a8c0@plab.local> Leica---they are work horses, like most histotechs!! :) Cheryl Miller Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histology@gradymem.org Sent: Friday, January 25, 2008 8:10 AM To: Patty Dunlop Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] if you could choose any microtome... I would buy a new Leica. Have been using one for years. Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org ----- Original Message ----- From: Patty Dunlop Date: Thursday, January 24, 2008 8:00 pm Subject: [Histonet] if you could choose any microtome... To: histonet@lists.utsouthwestern.edu > Hello, > > If you had the opportunity to order any microtome of your choice, > which one > would you order and why? Is there a better brand in terms of > equipment or > service? The only one I have ever used is a Microm motorized. I > did like > that one, but I have nothing to compare it to. > > Thanks, > Patty > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From gayle.callis <@t> bresnan.net Fri Jan 25 09:31:24 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Fri Jan 25 09:31:33 2008 Subject: [Histonet] O.C.T. embedding vs. freezing of unembedded samples References: <003401c85f46$e11b20a0$5a96d082@MrDarcy> Message-ID: <002d01c85f67$5810def0$6501a8c0@DHXTS541> The purpose of OCT is to support the tissue during cryotomy. I have done it both ways and anything I have snap frozen without embedded will be embedded in OCT prior to sectioning. Snap freezing of tissue can be done either way, but we like OCT surrounding the tissue for sectioning, it also gives the handle for those using brush technique versus antiroll device. If we do not fill mouse lung and then support it with OCT, the sections and cryotomy are terrible. We also like the OCT around the tissues (blocks) for -80C storage for murine animal model immunostaining purposes. Gayle M. Callis HT/HTL/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Mareike Heimann" To: Sent: Friday, January 25, 2008 4:39 AM Subject: [Histonet] O.C.T. embedding vs. freezing of unembedded samples Hi there, I have been wondering about the advantages (or disadvantages) of embedding tissue in O.C.T. before snap-freezing in liquid nitrogen when compared to snap-freezing the "naked" tissue. I do use O.C.T. before freezing, but when recently asked why, I found no better answer than "it seems to be what most people do..." I would be most grateful for any explanations! (My tissue blocks are used for immunohistochemical stainings later on) Regards, Mareike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Fri Jan 25 09:35:17 2008 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Fri Jan 25 09:35:04 2008 Subject: [Histonet] Re: HER2 fixation time In-Reply-To: <62789.3461.qm@web61217.mail.yahoo.com> References: <62789.3461.qm@web61217.mail.yahoo.com> Message-ID: <7F6B678A32B0564196138E6B3101996A013BADAB@EVS1.clinlan.local> I think we are forgetting that this "qualitative result" has large therapeutic implications for the patient. If your results are different from mine, are we really doing our best for the patient? This is the whole premise behind the attempt to standardize and frankly you have to start somewhere, so why not fixation? I for one am fine with the additional headache of working out the fixation tracking if it helps just one individual. But like many of you I do hope that the fixation time limit is expanded beyond 48 hours. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, January 24, 2008 2:37 PM To: Dawson, Glen Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: HER2 fixation time Is now "Big brother" in the histology business? All this standardization to get to a QUALITATIVE result? Where are the "standard" photomicrographs printed identically with the same standard color quality prints to compare with? I know of two color blind pathologists and three with poor vision. Ren? J. "Dawson, Glen" wrote: All, Once we all agree to fix our tissues for EXACTLY the same amount of time in the same fixative (supplied by the same vendor of course), what do we do with all of the other factors involved to reach this pipe dream of national standardization? The next step must be a requirement for all of us to work in the same hermetically sealed, mass produced "pre-fab" labs that can all provide identical water sources, temperatures, humidity, elevation/pressure, etc... We must all agree to use identical antigen retrieval methods, kits/detections, chromagens, etc... provided by the same "super vendor" with the same lot numbers. This may be feasable with all the buyouts in our industry but remains highly unlikely. Lastly, we must all agree to invite in only the same gremlins since they seem to thrive in so many laboratory environments. My point is, the new CAP HER2 guidlines are a fruitless attempt to standardize a procedure that has too many factors involved to achieve true, nation-wide standardization. The current discussion on the fixation aspect needs to be tempered by the fact that it is still only one factor in many. Does anyone else get that sick feeling that this is just the tip of the iceburg and calls for pre-fab kits and national standardization for many other tests are just around the corner? Things that make you go HMMMMMM, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Jan 25 09:48:58 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 25 09:49:13 2008 Subject: [Histonet] Re: HER2 fixation time In-Reply-To: <7F6B678A32B0564196138E6B3101996A013BADAB@EVS1.clinlan.local> Message-ID: <891355.22254.qm@web61217.mail.yahoo.com> Nobody is against (at least not I) a fixation standard. What I am trying to find out is the rationale for that specific standard. Why 48 and not more hours? What would be the QUALITATIVE difference of the results? When I emphazise qualitative is not because of disdain, but because of the fact that a qualitative result is less "demanding" of the way it is reached. Quantitative results NEED stringent procedures and quality control because at the end the amount to be quatified can be compromised if the protocol is not followed as prescribed. That is all, not because the quality (more or less + cells and score) is to be considered as less important to the patient. Ren? J. "Della Speranza, Vinnie" wrote: I think we are forgetting that this "qualitative result" has large therapeutic implications for the patient. If your results are different from mine, are we really doing our best for the patient? This is the whole premise behind the attempt to standardize and frankly you have to start somewhere, so why not fixation? I for one am fine with the additional headache of working out the fixation tracking if it helps just one individual. But like many of you I do hope that the fixation time limit is expanded beyond 48 hours. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, January 24, 2008 2:37 PM To: Dawson, Glen Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: HER2 fixation time Is now "Big brother" in the histology business? All this standardization to get to a QUALITATIVE result? Where are the "standard" photomicrographs printed identically with the same standard color quality prints to compare with? I know of two color blind pathologists and three with poor vision. Ren? J. "Dawson, Glen" wrote: All, Once we all agree to fix our tissues for EXACTLY the same amount of time in the same fixative (supplied by the same vendor of course), what do we do with all of the other factors involved to reach this pipe dream of national standardization? The next step must be a requirement for all of us to work in the same hermetically sealed, mass produced "pre-fab" labs that can all provide identical water sources, temperatures, humidity, elevation/pressure, etc... We must all agree to use identical antigen retrieval methods, kits/detections, chromagens, etc... provided by the same "super vendor" with the same lot numbers. This may be feasable with all the buyouts in our industry but remains highly unlikely. Lastly, we must all agree to invite in only the same gremlins since they seem to thrive in so many laboratory environments. My point is, the new CAP HER2 guidlines are a fruitless attempt to standardize a procedure that has too many factors involved to achieve true, nation-wide standardization. The current discussion on the fixation aspect needs to be tempered by the fact that it is still only one factor in many. Does anyone else get that sick feeling that this is just the tip of the iceburg and calls for pre-fab kits and national standardization for many other tests are just around the corner? Things that make you go HMMMMMM, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From JWEEMS <@t> sjha.org Fri Jan 25 10:07:53 2008 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Jan 25 10:08:07 2008 Subject: [Histonet] Re: HER2 fixation time In-Reply-To: <7F6B678A32B0564196138E6B3101996A013BADAB@EVS1.clinlan.local> References: <62789.3461.qm@web61217.mail.yahoo.com> <7F6B678A32B0564196138E6B3101996A013BADAB@EVS1.clinlan.local> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F4A65@sjhaexc02.sjha.org> Our Medical Director called to find out how this time was determined. It was just randomly chosen - obviously without consideration of the labs that are not open on Sat. If everyone starts changing things - even with documentation - we lose the attempt at standardization. Just have someone remove the blocks from the processor and everyone will have followed the standards! My 2 cents again... J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Della Speranza, Vinnie Sent: Friday, January 25, 2008 10:35 AM To: Rene J Buesa; Dawson, Glen Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: HER2 fixation time I think we are forgetting that this "qualitative result" has large therapeutic implications for the patient. If your results are different from mine, are we really doing our best for the patient? This is the whole premise behind the attempt to standardize and frankly you have to start somewhere, so why not fixation? I for one am fine with the additional headache of working out the fixation tracking if it helps just one individual. But like many of you I do hope that the fixation time limit is expanded beyond 48 hours. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, January 24, 2008 2:37 PM To: Dawson, Glen Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: HER2 fixation time Is now "Big brother" in the histology business? All this standardization to get to a QUALITATIVE result? Where are the "standard" photomicrographs printed identically with the same standard color quality prints to compare with? I know of two color blind pathologists and three with poor vision. Ren? J. "Dawson, Glen" wrote: All, Once we all agree to fix our tissues for EXACTLY the same amount of time in the same fixative (supplied by the same vendor of course), what do we do with all of the other factors involved to reach this pipe dream of national standardization? The next step must be a requirement for all of us to work in the same hermetically sealed, mass produced "pre-fab" labs that can all provide identical water sources, temperatures, humidity, elevation/pressure, etc... We must all agree to use identical antigen retrieval methods, kits/detections, chromagens, etc... provided by the same "super vendor" with the same lot numbers. This may be feasable with all the buyouts in our industry but remains highly unlikely. Lastly, we must all agree to invite in only the same gremlins since they seem to thrive in so many laboratory environments. My point is, the new CAP HER2 guidlines are a fruitless attempt to standardize a procedure that has too many factors involved to achieve true, nation-wide standardization. The current discussion on the fixation aspect needs to be tempered by the fact that it is still only one factor in many. Does anyone else get that sick feeling that this is just the tip of the iceburg and calls for pre-fab kits and national standardization for many other tests are just around the corner? Things that make you go HMMMMMM, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Malcolm.McCallum <@t> tamut.edu Fri Jan 25 10:13:13 2008 From: Malcolm.McCallum <@t> tamut.edu (Malcolm McCallum) Date: Fri Jan 25 10:14:08 2008 Subject: [Histonet] if you could choose any microtome... References: <80ab7bc60801241759k74f2f4b1gab17aab7ad2f26f6@mail.gmail.com> <001201c85f63$25b26b30$3d02a8c0@plab.local> Message-ID: A functional rotary! I'm stuck using a sliding one right now. I hate it, but such is life! Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-223-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html VISIT HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org A New Journal Published in Partnership with Partners in Amphibian and Reptile Conservation and the World Congress of Herpetology. Spring Teaching Schedule & Office Hours: Genetics: W 6:00 to 9:40pm Herpetology: TR 10:00-11:40am Histology: MW 1:00-2:40pm Seminar: T 2:30-3:30pm Office Hours: M: 3:30-5:00pm T: 11:40-1:00pm; 3:30-5:00pm W: 4:00-6:00pm "We live in a time when lemonade is made with artificial flavoring, and furnisher polish is made with fresh lemons." -Alfred E. Neuman -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cheri Miller Sent: Fri 1/25/2008 9:01 AM To: histology@gradymem.org; 'Patty Dunlop' Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] if you could choose any microtome... Leica---they are work horses, like most histotechs!! :) Cheryl Miller Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histology@gradymem.org Sent: Friday, January 25, 2008 8:10 AM To: Patty Dunlop Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] if you could choose any microtome... I would buy a new Leica. Have been using one for years. Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org ----- Original Message ----- From: Patty Dunlop Date: Thursday, January 24, 2008 8:00 pm Subject: [Histonet] if you could choose any microtome... To: histonet@lists.utsouthwestern.edu > Hello, > > If you had the opportunity to order any microtome of your choice, > which one > would you order and why? Is there a better brand in terms of > equipment or > service? The only one I have ever used is a Microm motorized. I > did like > that one, but I have nothing to compare it to. > > Thanks, > Patty > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lldewe <@t> gmail.com Fri Jan 25 10:25:16 2008 From: lldewe <@t> gmail.com (Loralei Dewe) Date: Fri Jan 25 10:25:36 2008 Subject: [Histonet] if you could choose any microtome... In-Reply-To: References: <80ab7bc60801241759k74f2f4b1gab17aab7ad2f26f6@mail.gmail.com> <001201c85f63$25b26b30$3d02a8c0@plab.local> Message-ID: <7173d3c00801250825v54ec3e43l102670bf2d2fa516@mail.gmail.com> I love the Microm 355 motorized!! I've purchased 2 of them in my career!! Loralei Dewe On Jan 25, 2008 8:13 AM, Malcolm McCallum wrote: > A functional rotary! > I'm stuck using a sliding one right now. > I hate it, but such is life! > > Malcolm L. McCallum > Assistant Professor > Department of Biological Sciences > Texas A&M University Texarkana > 2600 Robison Rd. > Texarkana, TX 75501 > O: 1-903-223-3134 > H: 1-903-791-3843 > Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html > VISIT HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org > A New Journal Published in Partnership with Partners in Amphibian and > Reptile Conservation > and the World Congress of Herpetology. > > Spring Teaching Schedule & Office Hours: > Genetics: W 6:00 to 9:40pm > Herpetology: TR 10:00-11:40am > Histology: MW 1:00-2:40pm > Seminar: T 2:30-3:30pm > Office Hours: > M: 3:30-5:00pm > T: 11:40-1:00pm; 3:30-5:00pm > W: 4:00-6:00pm > > "We live in a time when lemonade is made with artificial flavoring, and > furnisher polish is made with fresh lemons." > -Alfred E. Neuman > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cheri Miller > Sent: Fri 1/25/2008 9:01 AM > To: histology@gradymem.org; 'Patty Dunlop' > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] if you could choose any microtome... > > Leica---they are work horses, like most histotechs!! :) > > Cheryl Miller > Histology Supervisor > Physicians Laboratory,P.C. > Omaha, Ne. > 402 738 5052 > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > histology@gradymem.org > Sent: Friday, January 25, 2008 8:10 AM > To: Patty Dunlop > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] if you could choose any microtome... > > I would buy a new Leica. Have been using one for years. > > Angie Barnett, HTL(ASCP) > Grady Memorial Hospital > Pathology Department > 405/224-2258 > histology@gradymem.org > > > ----- Original Message ----- > From: Patty Dunlop > Date: Thursday, January 24, 2008 8:00 pm > Subject: [Histonet] if you could choose any microtome... > To: histonet@lists.utsouthwestern.edu > > > Hello, > > > > If you had the opportunity to order any microtome of your choice, > > which one > > would you order and why? Is there a better brand in terms of > > equipment or > > service? The only one I have ever used is a Microm motorized. I > > did like > > that one, but I have nothing to compare it to. > > > > Thanks, > > Patty > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. > If > you are not the addressee intended / indicated or agent responsible for > delivering it to the addressee, you are hereby notified that you are in > possession of confidential and privileged information. Any dissemination, > distribution, or copying of this e-mail is strictly prohibited. If you > have > received this message in error, please notify the sender immediately and > delete this email from your system. > > > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. > If you are not the addressee intended / indicated or agent responsible for > delivering it to the addressee, you are hereby notified that you are in > possession of confidential and privileged information. Any dissemination, > distribution, or copying of this e-mail is strictly prohibited. If you have > received this message in error, please notify the sender immediately and > delete this email from your system. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From fudo <@t> ufl.edu Fri Jan 25 10:32:28 2008 From: fudo <@t> ufl.edu (FU,DONGTAO) Date: Fri Jan 25 10:32:40 2008 Subject: [Histonet] PECAM-1 antibody Message-ID: <55822746.183381201278748040.JavaMail.osg@osgjas02.cns.ufl.edu> Hi, all I have been working on PECAM-1 antibody(Cat# sc-1506, goat ant-imouse ) from Santa Cruz on mouse,human and rat tissue, I tried a lot of methods(ABC-Elite method and HRP polyer method) and a lot of different AR, but could not let it work. The antibody stained everything including endothelial cell which I am really interested in. Is anybody here has experience on this antibody? Many tahanks, Ann Dongtao Fu MD, Ph.D Lab Manager Molecular Pathology core Dept. of Pathology Lab phone: 352-273-7752 Lab FAX: 352-273-7755 Lab address: D11-50 PO Box: 100275 1600 SW Archer Road University of Flodrida Gainesville, FL 32610 From vvancleave <@t> hendrickhealth.org Fri Jan 25 10:46:11 2008 From: vvancleave <@t> hendrickhealth.org (Vancleave, Vince) Date: Fri Jan 25 10:46:16 2008 Subject: [Histonet] They want me to apply as an allied health professional Message-ID: <740F77F6594C20458BDC2A4A500D8D41501655@EMAIL.hhs.hendrickhealth.org> Hello everyone, First, I would like to introduce myself as a newly certified pathologist assistant. My name is Vince Van Cleave (Steven is my first name) and I was OJT. I have been doing gross examination since 2002. Here is the deal. Since I have become certified, our pathologists want to get me doing gross at the hospital across the street as well as our private lab. It is something that I should be able to handle no sweat. The issue that we have been dealing with for the past 4 months is getting the hospital to allow me privelages to perform gross exam over there. To do this they want me to go through the entire allied health professionals credentialing procedures. This means, CPR certification, liability insurance, privelages request forms, credentialing forms (the same ones the docs fill out), and etc. I did a two week internship at UTMB and it took me about 2 weeks to get approved for doing examinations over there. I mean, I did criminal history, volunteer app, a "yes, I had my shots" thingy, references, and that was really about it. I think this is extremely assinine for the entire hospital administration to have a meeting to approve the possiblity, wait until their next meeting b/c it wasn't important as other problems they had to deal with, wait another two months for them to approve the "category," then wait two months to have another meeting to decide they don't have all the info they need, then wait until their next meeting to approve my credentials, then wait another two months for the next meeting to have them approve my application. Somebody please tell me that this isn't normal, b/c they feel like it absolutely has to be done that way. Of course, I should keep my mouth shut about my opinions I have had about the administration over there for ten years, to keep things professional here. haha. Well, anyone that might be able to help me out in the way of providing me with your experiences, that would be great. I appreciate you all and what you do for health care. God Bless. -Vince Van Cleave, PA(ASCP), HTL(ASCP), HT(ASCP) Vince Van Cleave, PA(ASCP)CM, HTL, HT Laboratory Manager and Pathologist Assistant (325)670-6528 Clinical Pathology Associates Abilene, TX PRIVILEGED AND CONFIDENTIAL NOTICE: The information contained in this e-mail may be confidential and/or privileged. This e-mail is intended to be reviewed initially by only the individual named above. If the reader of this e-mail is not the intended recipient or a representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail, or the information contained herein, is prohibited. If you have received this e-mail in error, notify the above sender, send the message back and then delete it. Thank you for your assistance. Hendrick Health System 1900 Pine St. Abilene, Texas 79601 - 325.670.2000 79601 - 325.670.2000 From rjbuesa <@t> yahoo.com Fri Jan 25 11:07:01 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 25 11:07:13 2008 Subject: [Histonet] They want me to apply as an allied health professional In-Reply-To: <740F77F6594C20458BDC2A4A500D8D41501655@EMAIL.hhs.hendrickhealth.org> Message-ID: <568910.76448.qm@web61220.mail.yahoo.com> Everything you write is essentially a personal choice you have to make. Dealing with such an administrative body will be of absolutely no use any other opinion even if they are standard as long as they contradict theirs. I think that you have to make that choice yourself: do you want to go along with what they want and if so do it, or you do not want to go through and then don't do it and get another job. Ren? J. "Vancleave, Vince" wrote: --------------------------------- Never miss a thing. Make Yahoo your homepage. From RSRICHMOND <@t> aol.com Fri Jan 25 11:14:56 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Fri Jan 25 11:15:11 2008 Subject: [Histonet] Re: HER2 fixation time Message-ID: >From the pathologist's viewpoint, underfixation is the problem in breast tissue, starting with the H & E. I'm seeing much more satisfactory sections now that overnight fixation is mandatory. The loss of turnaround time does put us at a disadvantage with the commercial labs, who are under no such constraints. I think that pathologists need to be passing this information on to the breast surgeons, that turnaround is going to be a bit slower. This point was emphasized to me in my residency forty years ago - that patients need to be told that, while their anxiety about their test results is real, that what they need is the right diagnosis and not the fast diagnosis, and that their pathologists are working with all possible speed. A couple of years ago I did a talk on IHC, and exported some photomicrographs from our very primitive photomicroscopy application to PhotoShop, so I could improve their appearance. I was amazed that I could take a weak-positive HER2 image and make it show anything from 0 to 3+ positivity with the proverbial click of the mouse. Bob Richmond Samurai Pathologist Knoxville TN From Keith.Danielson <@t> uphs.upenn.edu Fri Jan 25 11:15:56 2008 From: Keith.Danielson <@t> uphs.upenn.edu (Danielson, Keith) Date: Fri Jan 25 11:16:50 2008 Subject: [Histonet] Re: HER2 fixation time In-Reply-To: <891355.22254.qm@web61217.mail.yahoo.com> References: <7F6B678A32B0564196138E6B3101996A013BADAB@EVS1.clinlan.local> <891355.22254.qm@web61217.mail.yahoo.com> Message-ID: <84D11048BB18234D8A56474645E8EC020829EE10@uphsmbx5.UPHS.PENNHEALTH.PRV> Hello, The link below might be of interest. The article cites an interesting study by Arber in 2002 (Arber DA. Effect of prolonged formalin fixation on the immunohistochemical reactivity of breast markers. Appl Immunohistochem Mol Morphol. 2002;10:183-186.). He demonstrated that prolonged fixation of breast tissues in formalin for 7-14 days did not significantly affect immunoreactivity of Her2. I have not been able to get the full article yet -- I would appreciate receiving a PDF of it. http://findarticles.com/p/articles/mi_qa3725/is_200710/ai_n21099762 I am currently growing Her2 expressing cells in cell culture and plan to examine the effect of formalin fixation time and paraffin embedding on immnunoreactivity by IHC. Basically, I am making some control Her2 paraffin blocks for validation purposes. Keith Danielson, PhD Department of Pathology Pennsylvania Hospital Philadelphia, PA 19107 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, January 25, 2008 10:49 AM To: Della Speranza, Vinnie; Dawson, Glen Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: HER2 fixation time Nobody is against (at least not I) a fixation standard. What I am trying to find out is the rationale for that specific standard. Why 48 and not more hours? What would be the QUALITATIVE difference of the results? When I emphazise qualitative is not because of disdain, but because of the fact that a qualitative result is less "demanding" of the way it is reached. Quantitative results NEED stringent procedures and quality control because at the end the amount to be quatified can be compromised if the protocol is not followed as prescribed. That is all, not because the quality (more or less + cells and score) is to be considered as less important to the patient. Ren? J. "Della Speranza, Vinnie" wrote: I think we are forgetting that this "qualitative result" has large therapeutic implications for the patient. If your results are different from mine, are we really doing our best for the patient? This is the whole premise behind the attempt to standardize and frankly you have to start somewhere, so why not fixation? I for one am fine with the additional headache of working out the fixation tracking if it helps just one individual. But like many of you I do hope that the fixation time limit is expanded beyond 48 hours. Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, January 24, 2008 2:37 PM To: Dawson, Glen Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: HER2 fixation time Is now "Big brother" in the histology business? All this standardization to get to a QUALITATIVE result? Where are the "standard" photomicrographs printed identically with the same standard color quality prints to compare with? I know of two color blind pathologists and three with poor vision. Ren? J. "Dawson, Glen" wrote: All, Once we all agree to fix our tissues for EXACTLY the same amount of time in the same fixative (supplied by the same vendor of course), what do we do with all of the other factors involved to reach this pipe dream of national standardization? The next step must be a requirement for all of us to work in the same hermetically sealed, mass produced "pre-fab" labs that can all provide identical water sources, temperatures, humidity, elevation/pressure, etc... We must all agree to use identical antigen retrieval methods, kits/detections, chromagens, etc... provided by the same "super vendor" with the same lot numbers. This may be feasable with all the buyouts in our industry but remains highly unlikely. Lastly, we must all agree to invite in only the same gremlins since they seem to thrive in so many laboratory environments. My point is, the new CAP HER2 guidlines are a fruitless attempt to standardize a procedure that has too many factors involved to achieve true, nation-wide standardization. The current discussion on the fixation aspect needs to be tempered by the fact that it is still only one factor in many. Does anyone else get that sick feeling that this is just the tip of the iceburg and calls for pre-fab kits and national standardization for many other tests are just around the corner? Things that make you go HMMMMMM, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From fudo <@t> ufl.edu Fri Jan 25 11:17:17 2008 From: fudo <@t> ufl.edu (FU,DONGTAO) Date: Fri Jan 25 11:17:36 2008 Subject: [Histonet] PECAM-1 antibody Message-ID: <1918536191.187131201281437791.JavaMail.osg@osgjas02.cns.ufl.edu> Hi, all I have been working on PECAM-1 antibody(Cat# sc-1506, goat ant-imouse ) from Santa Cruz on mouse,human and rat tissue, I tried a lot of methods(ABC-Elite method and HRP polyer method) and a lot of different AR, but could not let it work. The antibody stained everything including endothelial cell which I am really interested in. Is anybody here has experience on this antibody? Many tahanks, Ann Dongtao Fu MD, Ph.D Lab Manager Molecular Pathology core Dept. of Pathology Lab phone: 352-273-7752 Lab FAX: 352-273-7755 Lab address: D11-50 PO Box: 100275 1600 SW Archer Road University of Flodrida Gainesville, FL 32610 From doug <@t> ppspath.com Fri Jan 25 11:19:51 2008 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Fri Jan 25 11:20:33 2008 Subject: [Histonet] They want me to apply as an allied health professional In-Reply-To: <740F77F6594C20458BDC2A4A500D8D41501655@EMAIL.hhs.hendrickhealth.org> Message-ID: You may want to address this to the PA message board. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vancleave, Vince Sent: Friday, January 25, 2008 11:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] They want me to apply as an allied health professional Hello everyone, First, I would like to introduce myself as a newly certified pathologist assistant. My name is Vince Van Cleave (Steven is my first name) and I was OJT. I have been doing gross examination since 2002. Here is the deal. Since I have become certified, our pathologists want to get me doing gross at the hospital across the street as well as our private lab. It is something that I should be able to handle no sweat. The issue that we have been dealing with for the past 4 months is getting the hospital to allow me privelages to perform gross exam over there. To do this they want me to go through the entire allied health professionals credentialing procedures. This means, CPR certification, liability insurance, privelages request forms, credentialing forms (the same ones the docs fill out), and etc. I did a two week internship at UTMB and it took me about 2 weeks to get approved for doing examinations over there. I mean, I did criminal history, volunteer app, a "yes, I had my shots" thingy, references, and that was really about it. I think this is extremely assinine for the entire hospital administration to have a meeting to approve the possiblity, wait until their next meeting b/c it wasn't important as other problems they had to deal with, wait another two months for them to approve the "category," then wait two months to have another meeting to decide they don't have all the info they need, then wait until their next meeting to approve my credentials, then wait another two months for the next meeting to have them approve my application. Somebody please tell me that this isn't normal, b/c they feel like it absolutely has to be done that way. Of course, I should keep my mouth shut about my opinions I have had about the administration over there for ten years, to keep things professional here. haha. Well, anyone that might be able to help me out in the way of providing me with your experiences, that would be great. I appreciate you all and what you do for health care. God Bless. -Vince Van Cleave, PA(ASCP), HTL(ASCP), HT(ASCP) Vince Van Cleave, PA(ASCP)CM, HTL, HT Laboratory Manager and Pathologist Assistant (325)670-6528 Clinical Pathology Associates Abilene, TX PRIVILEGED AND CONFIDENTIAL NOTICE: The information contained in this e-mail may be confidential and/or privileged. This e-mail is intended to be reviewed initially by only the individual named above. If the reader of this e-mail is not the intended recipient or a representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail, or the information contained herein, is prohibited. If you have received this e-mail in error, notify the above sender, send the message back and then delete it. Thank you for your assistance. Hendrick Health System 1900 Pine St. Abilene, Texas 79601 - 325.670.2000 79601 - 325.670.2000 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kerry.l.crabb <@t> gsk.com Fri Jan 25 11:50:25 2008 From: kerry.l.crabb <@t> gsk.com (kerry.l.crabb@gsk.com) Date: Fri Jan 25 11:51:13 2008 Subject: [Histonet] Lab Remodel Message-ID: In our lab we have L shaped workstations and the techs like this arrangement. The microtome is in the corner of the L which is filled in some. From Dorothy.L.Webb <@t> HealthPartners.Com Fri Jan 25 12:01:29 2008 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Fri Jan 25 12:01:41 2008 Subject: [Histonet] microarrays Message-ID: <0E394B648E5284478A6CCB78E5AFDA2705635436@hpes1.HealthPartners.int> Does anyone know where tissue microarrays can be purchased, either the blocks or prepared slides? Also, did you hear the news that Ventana has truly been bought by Roche? It is official from Washington G2 reports, purchased for 3.4 billion at $89.50 a share! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From shive003 <@t> umn.edu Fri Jan 25 12:03:53 2008 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Jan 25 12:04:07 2008 Subject: [Histonet] $ rate for immunos References: Message-ID: <00e301c85f7c$a5ba90f0$b0065486@auxs.umn.edu> I charge by the slide, including all used for optimization. If the researchers supply the antibody, there is a reduction in charge per slide. Jan Shivers Senior Scientist Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu ----- Original Message ----- From: "louise renton" To: Sent: Friday, January 25, 2008 4:53 AM Subject: [Histonet] $ rate for immunos > Hi all, and a happy friday to you too! > > This has been asked me by a friend and I wasn't sure how to answer, so > i am consulting th egreat Histo oracle..... > If one is doing contract work, how do you calculate what to charge for > doing immunos? per slide, per hour? (materials supplied by researcher, > so no charge there for consumables) If there is optimization > involved?? > > Lokking forward both to hearing from y'all and to the weekend! > > -- > Louise Renton > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > "There are nights when the wolves are silent and only the moon howls". > George Carlin > No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rosenfeldtek <@t> hotmail.com Fri Jan 25 12:57:10 2008 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Fri Jan 25 12:57:24 2008 Subject: [Histonet] Chondrocyte antibody Message-ID: Someone was looking for a chnodrocyte antibody. I have used the rabbit polyclonal against human type-II collagen from Novocastra. Catalog number NCL-COLL-IIp. For formalin fixed paraffin embedded tissue, antigen retrieval with trypsin is required. Jerry Ricks Research Scientist University of Washington Department of Pathology _________________________________________________________________ Need to know the score, the latest news, or you need your Hotmail?-get your "fix". http://www.msnmobilefix.com/Default.aspx From jmahoney <@t> alegent.org Fri Jan 25 12:58:10 2008 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Fri Jan 25 12:59:03 2008 Subject: [Histonet] Lab Remodel In-Reply-To: References: Message-ID: <4799DCE20200003C000290A7@gwia.alegent.org> Any of you who know me will expect my reply to this question to be from a LEAN perspective. I'd suggest not building anything that is not movable and adaptable to change. As far as cutting and embedding stations, move them close together. Use movable tables. Look at your processes to get to the plan with the least movement of specimens and techs. I would not spend a lot of money on designing and building a work space that you may want to change when you learn about the advantages of LEAN. Jan Mahoney Alegent Health Omaha, NE >>> 01/25/2008 11:50 AM >>> In our lab we have L shaped workstations and the techs like this arrangement. The microtome is in the corner of the L which is filled in some. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jonathan.Arzt <@t> ARS.USDA.GOV Fri Jan 25 13:05:08 2008 From: Jonathan.Arzt <@t> ARS.USDA.GOV (Arzt, Jonathan) Date: Fri Jan 25 13:05:29 2008 Subject: [Histonet] Plum Island HT Job -- Last Chance Message-ID: <6B4AF69268EFAE4C80A786BA99F7A66701C5FE41@MD-MAIL-01.ARSNET.ARS.USDA.GOV> The last day to apply for this position is Jan 28, 2008. If it is not filled this time, it will not be re-advertised, and the position will be dissolved. BIODEFENSE HT/HTL POSITION AVAILABLE (PLUM ISLAND, NY/CT) A unique, full-time, permanent histotech. position is immediately available and advertised by the pathology division of the USDA, Animal Research Service on Plum Island, NY. The position will be offered at the grades of GS-7/9/11 conferring salary of $39K-$76K commensurate with experience and training. Benefits and promotion potential are excellent. Licensed HT and HTL preferred, but unlicensed candidates with appropriate experience will be considered and are encouraged to apply. The primary mission of the research group is investigation of veterinary diseases of potential threat to US agriculture interests. Currently the greatest emphases are directed towards characterizing the pathogenesis of foot-and-mouth disease and classical swine fever. The Plum Island Animal Disease Center is located off the East end of Long Island, NY. Transport to and from the island is provided free to employees aboard government-operated ferries servicing Orient, NY and Old Saybrook, CT. Rural and suburban communities are abundant near both the NY and CT sides of the ferries with access to scenic beaches and vineyards. New York City and Boston are both within 2-4hrs drive. Activities will be varied but will include conventional histotech work with paraffin-embedded and frozen tissues, immunohistochemistry, in-situ hybridization, confocal microscopy, and assistance with animal necropsies and sample collection. The position is currently advertised at: www.usajobs.com as announcement #: ARS-X8E-0001R . Open period for application is through January 28, 2008. For more information, email to: Jonathan.Arzt@ARS.USDA.GOV If applying, please email application materials directly to this address in addition to filing the application as described at USAJOBS. Note: this is an informational notice only and does not constitute an official advertisement of any position. From Warren_Eddings <@t> ssmhc.com Fri Jan 25 13:19:08 2008 From: Warren_Eddings <@t> ssmhc.com (Warren_Eddings@ssmhc.com) Date: Fri Jan 25 13:19:39 2008 Subject: [Histonet] antibody validation Message-ID: got a few responses for antibody validation b tkanks warren _________________________________________________________________ Confidentiality Notice: This email message, including any attach may contain con unauthorized review, use, discl prohibited. If you are not the intended recipien the sender by reply email and destroy all copies of the o message. From Linke_Noelle <@t> Allergan.com Fri Jan 25 13:52:00 2008 From: Linke_Noelle <@t> Allergan.com (Linke_Noelle) Date: Fri Jan 25 13:52:26 2008 Subject: [Histonet] PECAM-1 antibody In-Reply-To: <1918536191.187131201281437791.JavaMail.osg@osgjas02.cns.ufl.edu> Message-ID: <5C3DA4BE34AA0641BAA10A7C1478B60527D09A@IRMAIL132.irvine.allergan.com> Ahhhh....the infamous 'dead goat antibody' again raises it's ugly head!! Ann....back in the day that antibody worked like a dream on rat and mouse, and then their goat died and it has not worked since.... If you ever find one that does work, you will be the queen of the Histonet! DEAR VENDORS...PLEASE make us a CD31 antibody that works on rats and mice! Noelle No?lle Linke, MS, HTL(ASCP)QIHC Allergan, Inc 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5568 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of FU,DONGTAO Sent: Friday, January 25, 2008 9:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PECAM-1 antibody Hi, all I have been working on PECAM-1 antibody(Cat# sc-1506, goat ant-imouse ) from Santa Cruz on mouse,human and rat tissue, I tried a lot of methods(ABC-Elite method and HRP polyer method) and a lot of different AR, but could not let it work. The antibody stained everything including endothelial cell which I am really interested in. Is anybody here has experience on this antibody? Many tahanks, Ann Dongtao Fu MD, Ph.D Lab Manager Molecular Pathology core Dept. of Pathology Lab phone: 352-273-7752 Lab FAX: 352-273-7755 Lab address: D11-50 PO Box: 100275 1600 SW Archer Road University of Flodrida Gainesville, FL 32610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LBUSTAMANTE <@t> cvm.tamu.edu Fri Jan 25 13:57:13 2008 From: LBUSTAMANTE <@t> cvm.tamu.edu (Lin Bustamante) Date: Fri Jan 25 13:58:05 2008 Subject: [Histonet] Acid Fast Bacteria/ Ziehl Neelsen Message-ID: <4799EAB9.EB3B.00B9.0@cvm.tamu.edu> Can long storage of wet tissue (over 6 months) in 70 % Etoh diminish the reaction for positive infected tissue with Mycobacterium tuberculosis? Thank you. Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab. Supervisor Dept. of Veterinary Integrative Biosciences Texas A&M University College Station, TX 77843-4458 (979)845-3177 From dcrippen <@t> buckinstitute.org Fri Jan 25 14:36:57 2008 From: dcrippen <@t> buckinstitute.org (Danielle Crippen) Date: Fri Jan 25 14:37:12 2008 Subject: [Histonet] plastic sectioning for light microscopy-HELP!! Message-ID: Dear Histo-experts, This is a first for me as I have only done ultrathin sectioning for EM. I need 0.5-1um serial sections for light microscopy. My main problem at this point is transferring the ribbons to a glass (plus) slide. No matter how I try to attempt the transfer, the ribbon comes apart and/or sections are lost or badly wrinkled. Any and all suggestions in this area are very welcome!! Additionally...once I actually get the sections onto the slide (which feels like it might be cause for a celebration at this point!!)...what are the most recommended H&E staining methods (these samples are embedded in EPON)? Again...I am a complete novice at plastic sectioning for light microscopy...so any suggestions are welcome...even the most trivial:-) A thousand thanks in advance!! danielle From rjbuesa <@t> yahoo.com Fri Jan 25 14:41:15 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jan 25 14:41:26 2008 Subject: [Histonet] Acid Fast Bacteria/ Ziehl Neelsen In-Reply-To: <4799EAB9.EB3B.00B9.0@cvm.tamu.edu> Message-ID: <337469.9999.qm@web61219.mail.yahoo.com> Theoretically it might. Remember that serous bacteria covering could be partially dissolved by EthOL. But try it, and let everybody know how it came about. Ren? J Lin Bustamante wrote: Can long storage of wet tissue (over 6 months) in 70 % Etoh diminish the reaction for positive infected tissue with Mycobacterium tuberculosis? Thank you. Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab. Supervisor Dept. of Veterinary Integrative Biosciences Texas A&M University College Station, TX 77843-4458 (979)845-3177 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From Heather.D.Renko <@t> osfhealthcare.org Fri Jan 25 14:59:38 2008 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Fri Jan 25 15:00:01 2008 Subject: [Histonet] Re: copath Message-ID: <40026EDDE64CDA47AB382C52619ACD3C0A09DCE1@pmc-rfd-mx01.intranet.osfnet.org> I am looking for some copath users to share some information. Can you email me personally if you have time and are in a sharing mood. Thank you in advance! Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From akemiat3377 <@t> yahoo.com Fri Jan 25 15:03:08 2008 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Fri Jan 25 15:03:20 2008 Subject: [Histonet] plastic sectioning for light microscopy-HELP!! In-Reply-To: Message-ID: <678999.942.qm@web31308.mail.mud.yahoo.com> Hi Danielle, Way back in my distant past I did GMA for OHSU Surgical Path Dept. We would put a few drops of acetone in our water. The section would spin a little on the water then settle. This helped to remove wrinkles. We would then place the slide on a slide warmer to further smooth out wrinkles and adhere the section. Hope this helps. Akemi Allison-Tacha BS, HT (ASCP) HTL Client Services Manager PhenoPath Laboratories 551 N 34th St., #100 Seattle, WA 98103 (206) 374-9000 akemi@phenopath.com http://www.phenopath.com --- Danielle Crippen wrote: > Dear Histo-experts, > > > > This is a first for me as I have only done ultrathin > sectioning for EM. > > > > > I need 0.5-1um serial sections for light microscopy. > My main problem at > this point is transferring the ribbons to a glass > (plus) slide. No > matter how I try to attempt the transfer, the ribbon > comes apart and/or > sections are lost or badly wrinkled. Any and all > suggestions in this > area are very welcome!! > > > > Additionally...once I actually get the sections onto > the slide (which > feels like it might be cause for a celebration at > this point!!)...what > are the most recommended H&E staining methods (these > samples are > embedded in EPON)? > > > > Again...I am a complete novice at plastic sectioning > for light > microscopy...so any suggestions are welcome...even > the most trivial:-) > > > > A thousand thanks in advance!! > > > > > > > > danielle > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jonesly <@t> mir.wustl.edu Fri Jan 25 15:32:30 2008 From: jonesly <@t> mir.wustl.edu (Jones, Lynne) Date: Fri Jan 25 15:32:41 2008 Subject: [Histonet] O.C.T. embedding vs. freezing of unembedded In-Reply-To: References: Message-ID: Hello - I'm not an expert by any stretch of the imagination, but I've taken to lightly glazing tissue with a thin layer of OCT (or equivalent) just before snap-freezing over liquid nitrogen. We sometimes keep tissues in a -80C freezer for 12 months (or longer) before sectioning. Even wrapping the brains in Parafilm and storing in short, squat cryo-containers led to surface dehydration after a couple months. The thin coating of OCT seems to protect the tissue during prolonged storage. FWIW - I float the tissue in a weigh boat in a Dewar that is half-filled with liquid N2 and covered to keep the vapor in. We work primarily with rodent tissues, often brain. When I dropped them directly in the N2, they tended to split or even brak into fragments. I also tried embedding smaller organs like thymus in OCT (and some of the thicker embedding compounds) using small disposable plastic molds, but it prolonged the freezing time considerably. Hope this helps, Lynne Message: 21 Date: Fri, 25 Jan 2008 11:39:00 -0000 From: "Mareike Heimann" Subject: [Histonet] O.C.T. embedding vs. freezing of unembedded samples To: Message-ID: <003401c85f46$e11b20a0$5a96d082@MrDarcy> Content-Type: text/plain; charset="iso-8859-1" Hi there, I have been wondering about the advantages (or disadvantages) of embedding tissue in O.C.T. before snap-freezing in liquid nitrogen when compared to snap-freezing the "naked" tissue. I do use O.C.T. before freezing, but when recently asked why, I found no better answer than "it seems to be what most people do..." I would be most grateful for any explanations! (My tissue blocks are used for immunohistochemical stainings later on) Regards, Mareike The materials in this message are private and may contain Protected Healthcare Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return mail. From akemiat3377 <@t> yahoo.com Fri Jan 25 15:42:22 2008 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Fri Jan 25 15:42:41 2008 Subject: [Histonet] plastic sectioning for light microscopy-HELP!! (H&E staining) In-Reply-To: Message-ID: <717246.74893.qm@web31305.mail.mud.yahoo.com> Hi Again, Sorry, I forgot our method for H&E's. Generally speaking, people would use Gills hematoxylin and Eosin or eosin/phloxine. Our pathologists didn't like the look of Gills III. In 1980, after doing extensive R&D, a light when on in my brain. Our nuclear staining on our GMA PAS's were beautiful. We oxidized in 10% periodic acid for 10 minutes for PAS. So, I tried doing the same with the H&E's on GMA's. The pathologists loved the results and said it looked like a regular H&E. I gave several workshops on this subject regarding GMA H&E's and special stain,s but I can't find my hand-out material. Sorry, it was back in the 80's. Here is the procedure from the cobwebs of my brain: 1. After adhering section to slide, place GMA slide directly into 10% Periodic Acid for 10 minutes. 2. Rinse in tap water for 1-2 minutes. 3. Place in Harris Hematoxylin for 15 to 20 minutes. 4. Rinse in tap water. 5. Quickly dip in 0.5% acid alcohol. 6. Rinse in tap water. 7. Blue in freshly prepared ammonia water. 8. Rinse in tap water for 10 minutes. 9. Counterstain in Eosin/Phloxine for desired length of time. You can start with 1-2 minutes. 10. Dehydrate through graded alcohols quickly. 11. Clear and coverslip. Hope this helps. Akemi Allison-Tacha BS, HT (ASCP) HTL Client Services Manager PhenoPath Laboratories 551 N 34th St., #100 Seattle, WA 98103 (206) 374-9000 akemi@phenopath.com http://www.phenopath.com --- Danielle Crippen wrote: > Dear Histo-experts, > > > > This is a first for me as I have only done ultrathin > sectioning for EM. > > > > > I need 0.5-1um serial sections for light microscopy. > My main problem at > this point is transferring the ribbons to a glass > (plus) slide. No > matter how I try to attempt the transfer, the ribbon > comes apart and/or > sections are lost or badly wrinkled. Any and all > suggestions in this > area are very welcome!! > > > > Additionally...once I actually get the sections onto > the slide (which > feels like it might be cause for a celebration at > this point!!)...what > are the most recommended H&E staining methods (these > samples are > embedded in EPON)? > > > > Again...I am a complete novice at plastic sectioning > for light > microscopy...so any suggestions are welcome...even > the most trivial:-) > > > > A thousand thanks in advance!! > > > > > > > > danielle > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Akemi Allison-Tacha, BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing Tele: (925)788-0900 E-Mail: akemiat3377@yahoo.com From pathology.histology <@t> gmail.com Fri Jan 25 15:56:05 2008 From: pathology.histology <@t> gmail.com (path lab) Date: Fri Jan 25 15:56:18 2008 Subject: [Histonet] Artifact question Message-ID: <73841640801251356r1354fd0cmd8083b0d7d509d@mail.gmail.com> Hello fellow Histonetters, I have posted the images referenced in this question as 'artifact image1', 'artifact image2', 'artifact image3', and 'artifact image4' to histonet.org. This is believed to be an artifact resembling very early deposition of mineral. The change is unusual for mineral deposits in its location and that it appears to affect nuclei before cytoplasm. Early basophilia of subepicardial individual cardiac myocytes is also seen (not shown). Note that the von Kossa staining is more widespread than the H&E lesion would appear to be. Does everyone agree that it is an artifact and does anyone know how it happens? Thanks in advance for your replies. From JWEEMS <@t> sjha.org Fri Jan 25 16:29:13 2008 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Jan 25 16:29:24 2008 Subject: [Histonet] Cytology GMS Stains Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32048F4A7E@sjhaexc02.sjha.org> I may have asked this before, but when you have a GMS on a cytology specimen, do you charge for 2 stains - one for the ThinPrep and one for the cell block? I see it as only one specimen and can charge only one 88312. I would appreciate your input. Have a good weekend everyone! Thanks, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From amosbrooks <@t> gmail.com Fri Jan 25 19:28:37 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Fri Jan 25 19:28:47 2008 Subject: [Histonet] O.C.T. embedding vs. freezing of unembedded Message-ID: <582736990801251728udf1ddc0i2433e262f2f7942@mail.gmail.com> Mareike, When you freeze the tissue without the OCT, you will eventually need to embed the tissue in OCT to cryosection it. When you do this you are putting warm OCT on frozen tissue which then thaws the tissue out before freezing it again. This will produce ice crystals worse than not snap freezing in the first place. If you intend to use the tissue for something other than cryosectioning, it will not be harmed by the OCT, however the cryosectioning can (will) be harmed by not freezing in OCT. Also, (as an afterthought) if there happens to be any temperature fluctuation in storage, the tissue will have a little more protection in the OCT. Hope this helps, Amos Brooks Message: 21 Date: Fri, 25 Jan 2008 11:39:00 -0000 From: "Mareike Heimann" Subject: [Histonet] O.C.T. embedding vs. freezing of unembedded samples To: Message-ID: <003401c85f46$e11b20a0$5a96d082 @MrDarcy> Content-Type: text/plain; charset="iso-8859-1" Hi there, I have been wondering about the advantages (or disadvantages) of embedding tissue in O.C.T. before snap-freezing in liquid nitrogen when compared to snap-freezing the "naked" tissue. I do use O.C.T. before freezing, but when recently asked why, I found no better answer than "it seems to be what most people do..." I would be most grateful for any explanations! (My tissue blocks are used for immunohistochemical stainings later on) Regards, Mareike From jmaass <@t> frii.com Fri Jan 25 22:34:06 2008 From: jmaass <@t> frii.com (Janet Maass) Date: Fri Jan 25 22:34:18 2008 Subject: [Histonet] Lab remodel References: <328CBAE62F31C642B422970E879DFADC01A80272@pcwex01> Message-ID: <002101c85fd4$b01ff910$0200a8c0@Janet03b999f> Dan I like a motorized work bench that has a shelf that can be pulled out for the floation bath. With a motorized bench one can sit or stand to microtome and change position with just a push of the up or down button with the floation bath full of water with no problems. Also can easily be adjusted for someone short or someone tall. Janet Maass From rjbuesa <@t> yahoo.com Sat Jan 26 11:01:14 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Jan 26 11:01:29 2008 Subject: [Histonet] HER2 fixation time (follow up). Message-ID: <446694.26805.qm@web61221.mail.yahoo.com> To all those who participated in the recent and sometimes "spirited" exchange of ideas on this subject, CAP TODAY (vol.22 No.1, January 2008) has just published an article by William Check ("Lifting the quality in IHC analysis") that is really worth reading. I hope you find it as interesting as I did. Ren? J. --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From carl.hobbs <@t> kcl.ac.uk Sat Jan 26 12:22:07 2008 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Sat Jan 26 12:22:35 2008 Subject: [Histonet] Re: PECAM-1 antibody Message-ID: <002401c86048$5c47cba0$4001a8c0@carlba65530bda> Interesting. Just out of interest, why do you want to use Pecam 1? I have not been able to get same sc Ab to work on FFPW sections of mouse/rat endothelial cells. However, I understand that it is also positive for non-endothelial cells so, is this the best marker for endothelial cells? Any views on using anti CD34, Aquaporin, VWF on mouse/rat FFPW sections to detect capillaries? Can anti laminin be used instead? Carl From contact <@t> excaliburpathology.com Sat Jan 26 13:07:17 2008 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Sat Jan 26 13:07:30 2008 Subject: [Histonet] plastic sectioning forlight microscopy-HELP!! Message-ID: <120990.89254.qm@web50111.mail.re2.yahoo.com> Your description sounds like you are trying to take the sections right from the microtome to the slide. Try placing the sections on a room temperature waterbath containing weak ammonia. Put 4-5 drops of ammonium hydroxide in distilled water. Pick the sections up by dipping the slide in the water and bringing it up under the sections. This is opposite of picking ultra thin sections up on grids from the tiny waterbath on the knife on the ultra microtome. Dry the sections before staining. 0.1% Toluidine blue is a good fast stain to see if you are where you want to be in the tissue before proceeding with other stains. Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-759-3953 contact@excaliburpathology.com www.excaliburpathology.com From liz <@t> premierlab.com Sat Jan 26 13:17:07 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Sat Jan 26 13:17:18 2008 Subject: [Histonet] Re: PECAM-1 antibody In-Reply-To: References: Message-ID: Carl I happen to have the lot number of the santa cruz antibody that works in paraffin. Its quite a nice antibody and whats also nice is that is cross reacts across multiple species, in my hands it stains human, mouse, rat, rabbit, canine, and porcine tissues, basically everything I have tried it on. I don't know what I'm going to do when I run out of it, I use it at a concentration of 1:800 so it will last me for awhile yet. We also will use CD34 and Factor VII to stain vessels, but I suspect that a good portion of my clients like to use the CD-31 or PECAM-1 because of its use in the literature. Serotec has a nice CD34 that works in murine FFPE tissues and we also use the dako CD34 on human tissue samples, but I'm not aware of an antibody to CD34 that cross reacts with rat. We have factor VIII from both abcam and dako, they are nice antibodies that cross react across multiple species too. I think I read somewhere that very new cappilaries do not stain with factor VIII and thats why people prefer the CD-31 also. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carl Hobbs Sent: Saturday, January 26, 2008 11:31 AM To: Histonet Subject: [Histonet] Re: PECAM-1 antibody Interesting. Just out of interest, why do you want to use Pecam 1? I have not been able to get same sc Ab to work on FFPW sections of mouse/rat endothelial cells. However, I understand that it is also positive for non-endothelial cells so, is this the best marker for endothelial cells? Any views on using anti CD34, Aquaporin, VWF on mouse/rat FFPW sections to detect capillaries? Can anti laminin be used instead? Carl _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LuckG <@t> empirehealth.org Sat Jan 26 13:31:03 2008 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Sat Jan 26 13:31:17 2008 Subject: [Histonet] Leitz Microtome (mod.#1512) Message-ID: <6BB8BC4519AAB844B174FC739A679BBCCEFFDA@IRMEXCH01.irm.inhs.org> Hello All, Am looking for a model 1512 Leitz microtome, that be sitting around unused/in storage, that somebody may in interested in parting with. Thanks, Greg Greg Luck, B.S., HT(ASCP) Anatomic Pathology Supervisor Deaconess Med Cntr 800 W. 5th Ave Spokane, WA 99204 Offc 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmc.org From koellingr <@t> comcast.net Sat Jan 26 13:53:21 2008 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Sat Jan 26 13:53:36 2008 Subject: [Histonet] Re: PECAM-1 antibody Message-ID: <012620081953.1168.479B8FB1000AC1580000049022070215539D09020704040A0105@comcast.net> I agree completely with Carl, in asking the question about using CD31 which is a question that must always be asked for any project as far as just following conventional wisdom. Different needs and different data and according to your model might require different reagents. CD31 is kind of a default gold standard for endothelial cells but not perfect. And a more difficult antibody for mice/rats. Have used BDPharmingen rat-anti mouse CD31 on FFPE mouse tissue, WITH TYRAMIDE AMPLIFICATION, and it worked quite well. Detailed this several years back on HistoNet. Biotinylated rat Abs works on rats with same caveat of amplification need. Never did like that Santa Cruz CD31, even when popular back then, for specific and general reasons. Aquaporins in endothelial cells but also myocytes, basal layers of some epithelium, etc. Laminins not sure what that would do? or how helpful; is an extracellular matrix in basement membrane and other places. CD34 (mucosialin) might be great choic e for capillary endothelial cells but is in other places (hematopoietic stem cells). vWF might be great choice, works in FFPE nicely, but not ALL endothelial cells synthesize vWF. I've used VE-cadherin (vascular endothelial-cadherin) in FFPE mice for endothelial cells. In short there are some good choices out there for FFPE mouse/rat endothelium and while it might be a bit more tricky and problematic than easier and more focused targets with easier reagents, better than hoping for the reincarnation of that dumb goat that has been diefied, but whose anti-CD31 antibody containing serum I was always cautious and wary of in the first place. Ray Koelling PhenoPath Labs Seattle, WA -------------- Original message -------------- From: "Carl Hobbs" > Interesting. > Just out of interest, why do you want to use Pecam 1? > I have not been able to get same sc Ab to work on FFPW sections of > mouse/rat endothelial cells. > However, I understand that it is also positive for non-endothelial cells so, > is this the best marker for endothelial cells? > Any views on using anti CD34, Aquaporin, VWF on mouse/rat FFPW sections to > detect capillaries? > Can anti laminin be used instead? > Carl > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JGREWE <@t> OhioHealth.com Sat Jan 26 15:01:43 2008 From: JGREWE <@t> OhioHealth.com (JGREWE@OhioHealth.com) Date: Sat Jan 26 15:01:58 2008 Subject: [Histonet] Jacquelyn Grewe/Staff/OhioHealth is out of the office . Message-ID: I will be out of the office starting 01/25/2008 and will not return until 02/11/2008. I will return Tuesday November 13 at 11 PM and will respond to your message when I return. Thanks, Jackie From mickie25 <@t> netzero.net Sat Jan 26 16:01:44 2008 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Sat Jan 26 16:02:13 2008 Subject: [Histonet] Need information on Mohs billing In-Reply-To: <006001c859f6$65fceac0$6701a8c0@FSROGER> References: <006001c859f6$65fceac0$6701a8c0@FSROGER> Message-ID: Hello Cheryl, I work regularly with dermatologists to develop their Mohs practice and would be happy to consult directly if you find this useful. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 Web: www.mohshistotemp.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Friday, January 18, 2008 9:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need information on Mohs billing Hello all-- A Dermatologist friend is considering adding services to his already-working clinic. He is researching the feasability including the training to do Mohs--I've already pointed him to the Moh's college site and he's still interested. Now we need to figure out the numbers... Has anyone worked up a situation like this recently? Could anyone share with me the billing codes for Mohs--both technical and professional. Is there a cap on number of specimens? How does this work for the Derm Doc performing the procedure? Diagnosing the slides? Any input/education is welcome. Thank you! Cheryl Kerry, HT(ASCP) Full Staff 281.852.9457 Personal email: tkngflght@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From max_histo_00 <@t> yahoo.it Sat Jan 26 18:25:15 2008 From: max_histo_00 <@t> yahoo.it (Massimo) Date: Sat Jan 26 18:25:30 2008 Subject: [Histonet] Embryo epithelial tissue Message-ID: <603719.68921.qm@web23305.mail.ird.yahoo.com> Hello all, I have posted a photo called ?Epithelial_tissue_00.jpg? to "pictures@histonet.org" It is a picture coming from a prepared slide of a transversal section of a mouse embryo. Taken by my microscope and a Nikon Coolpix 4800 digital camera. I think it is about 20 days old. Stained with H&E. Enlargement 300x. I am learning , by myself, something about Histology. I wonder if anyone could help me by telling what the cells into the red circle are. Where can I find more information on such kind of tissues? Histology handbooks for instance. Any help would be greatly appreciated. Thanks in advance, Massimo --------------------------------- --------------------------------- L'email della prossima generazione? Puoi averla con la nuova Yahoo! Mail From carl.hobbs <@t> kcl.ac.uk Sun Jan 27 12:14:45 2008 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Sun Jan 27 12:15:34 2008 Subject: [Histonet] Cannabinoid receptor Abs Message-ID: <001f01c86110$7f76eb10$4001a8c0@carlba65530bda> Anyone out there use Abs against CB1/2 on frozen/pwax tissues/cells? I would be most grateful for your views. Thanks. Carlos From jstaruk <@t> masshistology.com Sun Jan 27 18:04:56 2008 From: jstaruk <@t> masshistology.com (jstaruk) Date: Sun Jan 27 18:05:17 2008 Subject: [Histonet] Kristensen's Sodium Formate/Formic acid recipe In-Reply-To: <012620081953.1168.479B8FB1000AC1580000049022070215539D09020704040A0105@comcast.net> Message-ID: <0902019EEC4E470892B0FFC176FD6845@JimPC> Does anyone have the recipe and procedure for Kristensen's Sodium Formate/Formic acid for demineralization of teeth? Thank you Jim _____________________ Jim Staruk Mass Histology Service www.masshistology.com From Barry.R.Rittman <@t> uth.tmc.edu Sun Jan 27 20:28:58 2008 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Sun Jan 27 20:29:12 2008 Subject: [Histonet] Kristensen's Sodium Formate/Formic acid recipe References: <0902019EEC4E470892B0FFC176FD6845@JimPC> Message-ID: 340 gms dried sodium formate 1700 ml. w/v 90% formic acid Distilled water to 10 liters. Fix as usual Rinse in running tap water after formalin fixation. Demineralize using agitation such as electronic stir bar. (Difficult to over deminerlize with this formula). Usually takes 3 to 5 times longer than 5% nitric acid. Wash well in running tap water . Sorry don't have a reference as I am at home but have used this for several years and prefer this to all other demineralization methods except EDTA. Worked well with many IHC techniques. Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of jstaruk Sent: Sun 1/27/2008 6:04 PM To: 'Histonet' Subject: [Histonet] Kristensen's Sodium Formate/Formic acid recipe Does anyone have the recipe and procedure for Kristensen's Sodium Formate/Formic acid for demineralization of teeth? Thank you Jim _____________________ Jim Staruk Mass Histology Service www.masshistology.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pdunlop720 <@t> gmail.com Sun Jan 27 20:32:39 2008 From: pdunlop720 <@t> gmail.com (Patty Dunlop) Date: Sun Jan 27 20:32:50 2008 Subject: [Histonet] advice about microtomes please! Message-ID: <80ab7bc60801271832w4113190l143854a9badf532d@mail.gmail.com> Hello again, I appreciate all responses about microtome of choice. I would like to ask for guidance on my specific situation. In my facility, I will be sectioning approximately 25-30 blocks/day of GI (upper and lower) tissue (mostly biopsies and some polyps) and possibly prostate. I have never used a manual microtome and do not know how repetitive it can be. In terms of ergonomics with worries of repetitive motion and carpel tunnel, should I try to convince my boss to get a motorized microtome, or will a manual suffice? I know that motorized microtomes are probably twice as expensive, and although I would feel more comfortable using the same one that I am used to, maybe it would be "going overboard" to get the motorized in my situation. Advice please! Patty From tkngflght <@t> yahoo.com Sun Jan 27 21:20:51 2008 From: tkngflght <@t> yahoo.com (Cheryl) Date: Sun Jan 27 21:21:12 2008 Subject: Motorized vs not RE: [Histonet] advice about microtomes please! In-Reply-To: <80ab7bc60801271832w4113190l143854a9badf532d@mail.gmail.com> References: <80ab7bc60801271832w4113190l143854a9badf532d@mail.gmail.com> Message-ID: <00da01c8615c$c9a728c0$6701a8c0@FSROGER> Hi Patti-- While I agree if you can afford one, get a motorized, how much is that carpal surgery and the associated problems vs. the price of a microtome? It isn't the end of the world if you don't get it, though. I've been a tech for 25 years and haven't had this resource available at most of my jobs. In cutting, I can rock n roll over 300 biopsy blocks with levels in a day (yes, good sections but only cutting--not getting up to do anything else). I haven't worked 100% on a histo bench this whole time but in the days of cutting like this every day--it was my SHOULDERS that got tired, not my wrists. If you're prone to carpal and a histotech--you're probably going to get it eventually. But you can slow it down and minimize the risk. (That department is called risk 'management', not risk elimination!!) The bigger issue is sitting high enough & close enough that you aren't holding your shoulders up with your neck muscles and that you alternate activities, stretch, take breaks...etc. The best microtome for your body might be the worst for someone else. Try to get a demo model and TRY IT. Pay attention to any aches or pains because although your body will adjust and get used to the situation--that doesn't mean the damage has stopped occuring. If it doesn't fit you--try the next model! PREVENTION is ALWAYS a better investment than banking on a reasonable cure. Taking care of your body is a big part. Make sure you take enough B vitamins (soft tissue support) drink enough water to support your own system (muscles and connective tissue) and any other supplements a good qualified nutrition/health food consultant might suggest for you. It is amazing how many of us don't drink water because we have to leave the lab to do so! Your state deparment of labor will have an ergonomics division and can send someone out to evalutate your setup to be sure you are maximizing the reduction of risk for repetitive motion and seat position for good health. One of my labs sent me through ergo training (Washington State) and I still use the manuals for setting up workstations in my office and when I travel and work in temp situations. I type 6-8 hours a day at a computer and can be on the phone over 40 hrs a week--same issues--and I'm still going strong (knock on wood!) Best wishes in your search! Cheryl Cheryl R. Kerry, HT(ASCP), BA Full Staff Inc. Staffing the lab - One GREAT tech at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone admin@fullstaff.org www.fullstaff.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patty Dunlop Sent: Sunday, January 27, 2008 8:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] advice about microtomes please! Hello again, I appreciate all responses about microtome of choice. I would like to ask for guidance on my specific situation. In my facility, I will be sectioning approximately 25-30 blocks/day of GI (upper and lower) tissue (mostly biopsies and some polyps) and possibly prostate. I have never used a manual microtome and do not know how repetitive it can be. In terms of ergonomics with worries of repetitive motion and carpel tunnel, should I try to convince my boss to get a motorized microtome, or will a manual suffice? I know that motorized microtomes are probably twice as expensive, and although I would feel more comfortable using the same one that I am used to, maybe it would be "going overboard" to get the motorized in my situation. Advice please! Patty _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.m.vanderloos <@t> amc.uva.nl Mon Jan 28 02:13:47 2008 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Mon Jan 28 02:14:07 2008 Subject: [Histonet] Re: HER2 fixation time Message-ID: <29bafb295d7c.295d7c29bafb@amc.uva.nl> Hi, During my workshop in Denver 'how to make a new antibody work for IHC?' I showed the audience a picture taken from the paper of Shi, Liu and Taylor in J Histochem Cytochem 55:105-109, 2007. In this paper there is the result shown of a fixation experiment from 6 hs to 30 days. According to the images presented there is no loss of Her2 within that time frame. Cheers, Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Fri, 25 Jan 2008 12:15:56 -0500 From: "Danielson, Keith" Subject: RE: [Histonet] Re: HER2 fixation time To: "Rene J Buesa" , "Della Speranza, Vinnie" , "Dawson, Glen" Cc: histonet@lists.utsouthwestern.edu Hello, The link below might be of interest. The article cites an interesting study by Arber in 2002 (Arber DA. Effect of prolonged formalin fixation on the immunohistochemical reactivity of breast markers. Appl Immunohistochem Mol Morphol. 2002;10:183-186.). He demonstrated that prolonged fixation of breast tissues in formalin for 7-14 days did not significantly affect immunoreactivity of Her2. I have not been able to get the full article yet -- I would appreciate receiving a PDF of it. http://findarticles.com/p/articles/mi_qa3725/is_200710/ai_n21099762 I am currently growing Her2 expressing cells in cell culture and plan to examine the effect of formalin fixation time and paraffin embedding on immnunoreactivity by IHC. Basically, I am making some control Her2 paraffin blocks for validation purposes. Keith Danielson, PhD Department of Pathology Pennsylvania Hospital Philadelphia, PA 19107 From louise.renton <@t> gmail.com Mon Jan 28 02:49:15 2008 From: louise.renton <@t> gmail.com (louise renton) Date: Mon Jan 28 02:49:26 2008 Subject: [Histonet] thanks Message-ID: Thanks to all who responded to my question re charging for immunos, much appreciated. haev a good week everyboby! -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From Luis.Chiriboga <@t> med.nyu.edu Mon Jan 28 06:59:15 2008 From: Luis.Chiriboga <@t> med.nyu.edu (Luis Chiriboga) Date: Mon Jan 28 06:59:50 2008 Subject: [Histonet] FW: Lab science gets a new face on Facebook! Message-ID: -----Original Message----- From: owner-mls-l@ms3.hunter.cuny.edu [mailto:owner-mls-l@ms3.hunter.cuny.edu]On Behalf Of Regina Linder Sent: Friday, January 25, 2008 3:25 PM To: mls-l@hunter.cuny.edu Subject: Fwd: Lab science gets a new face on Facebook! Hello Everyone: As the semester begins, check out this terrific site. "Labs are Vital" is on Facebook, with info on careers in all kinds of labs, scholarship contests, etc. You can download terrific posters to raise consciousness in your hospitals and your kid's schools. All the best, Dr. L If you are unable to see the email clearly, please follow the link below: [ please click here] Labs Are Vital officially joined the Facebook generation in December. In an effort to draw future professionals to clinical lab science, Labs Are Vital has launched a new recruitment campaign on Facebook with information on educational and career opportunities, and a $2,500 scholarship contest. An announcement letter about the Facebook campaign and scholarship opportunities was also mailed to over 10,000 high school counselors and science department heads. Contest entrants may submit a video, advertisement or T-shirt design that encourages others to consider careers in laboratory medicine. Semifinalist submissions will be posted online, where Facebook members will vote on those that most creatively illustrate why clinical lab science is cutting edge and how laboratorians make a difference. Abbott is joined in this effort by ASCLS, APHL and Blood Systems, Inc. Equipment donation call for entries! Labs Are Vital invites all accredited CLS/CLTS programs to apply for our latest round of equipment donations. So far, 43 programs have been selected to receive state-of-the-art diagnostic equipment, supplies and service through the Labs Are Vital $1 million donation program. You'll find winner profiles, and have the opportunity to apply for the next round of donations. But don't wait-applications are due March 31, 2008! For application, click here. The polls have closed and the results are in! Thanks so much to the many of you who participated in our recent Labs Are Vital survey. The response rate was gratifying. While we are still in the process of analyzing the data, the top-line response lets us know that the majority of you have been instrumental in passing the word about Labs Are Vital, which has resulted in many new members. And your suggestions about what you would like us to add will only help improve Labs Are Vital and make it even better in 2008. We'll be holding our drawing for 100 Labs Are Vital lab coats in mid-February, and will announce the winners shortly thereafter. We'll also be sharing more of the survey results with you-the lab professionals who keep health care moving forward. Send a copy of this e-mail to a colleague Unsubscribe If you are unable to see this email clearly, please click here Download Labs Are Vital posters Our Labs Are Vital posters not only celebrate the remarkable contributions of lab professionals, they've also proven to be quite popular with laboratorians and the general public alike. To download one or more of this popular series, click here. Powered by NetDyaLog _________________________________________________________________________ Please address your reply directly to mls-l@hunter.cuny.edu. For help, send a message to majordomo@hunter.cuny.edu with the word "help" in the body of your message. To unsubscribe, send a message to majordomo@hunter.cuny.edu with following command in the body of your message: unsubscribe mls-l your_email_address _________________________________________________________________________ From maria.geraci-erck <@t> spcorp.com Mon Jan 28 07:02:39 2008 From: maria.geraci-erck <@t> spcorp.com (Geraci-Erck, Maria) Date: Mon Jan 28 07:02:49 2008 Subject: [Histonet] Epstein Barr Virus Ab and Control Tissue Message-ID: Dear Histonetters, I need to label monkey tissue for Epstein Barr Virus. Does anyone out there have experience with this procedure? I would like to know if there is a particular Ab that would identify an active infection. There are a number of Ab's out there and I was wondering if there is a favorite that gives consistent results. I am also in need of positive control tissue. I have e-mailed Pantomics and unfortunately, they did not have a block. I found a company that sells slides, but in my opinion, they are pricey. Any guidance would be appreciated. Maria Maria Geraci-Erck Schering-Plough Research Institute Special Techniques Laboratory 556 Morris Avenue, Bldg. S-12 Summit, New Jersey 07901-1002 Phone: (908) 473-4284 Fax: (908) 473-4420 maria.geraci-erck@spcorp.com ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From ryan <@t> upei.ca Mon Jan 28 11:25:31 2008 From: ryan <@t> upei.ca (Dr. Catherine L. Ryan) Date: Mon Jan 28 11:28:28 2008 Subject: [Histonet] Camera Lucida Message-ID: <479DD7C1.12905.349CDCCF@localhost> Hello everyone: I am interested in purchasing a camera lucida attachment for my scope.Does anyone have one they are no longer using and would be interested in selling? Cathy Ryan Dr. Catherine L. Ryan Department of Psychology University of Prince Edward Island Charlottetown, P.E.I., Canada, C1A 4P3 Ph# (902)566-0323/ FAX#(902)628-4359 email: RYAN@UPEI.CA From rjbuesa <@t> yahoo.com Mon Jan 28 11:36:54 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jan 28 11:37:06 2008 Subject: [Histonet] Camera Lucida In-Reply-To: <479DD7C1.12905.349CDCCF@localhost> Message-ID: <27813.7341.qm@web61224.mail.yahoo.com> I have seen one or two in e-Bay Ren? J. "Dr. Catherine L. Ryan" wrote: Hello everyone: I am interested in purchasing a camera lucida attachment for my scope.Does anyone have one they are no longer using and would be interested in selling? Cathy Ryan Dr. Catherine L. Ryan Department of Psychology University of Prince Edward Island Charlottetown, P.E.I., Canada, C1A 4P3 Ph# (902)566-0323/ FAX#(902)628-4359 email: RYAN@UPEI.CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From innvx <@t> sbcglobal.net Mon Jan 28 12:12:31 2008 From: innvx <@t> sbcglobal.net (INNOVEX BIOSCIENCES) Date: Mon Jan 28 12:12:42 2008 Subject: [Histonet] Animal Tissue IHC Staining Workshop Message-ID: <342772.47862.qm@web82013.mail.mud.yahoo.com> Animal Tissue IHC Staining wet Workshop will be conducted in Chicago on Feburary 22, 23, in Chicago. For detailed information please contact Innovex at innvx@sbcglobal.net. From Kathy.Bucknell <@t> leica-microsystems.com Mon Jan 28 12:13:25 2008 From: Kathy.Bucknell <@t> leica-microsystems.com (Kathy.Bucknell@leica-microsystems.com) Date: Mon Jan 28 12:13:53 2008 Subject: [Histonet] Re: Histonet Digest, Vol 50, Issue 39 Message-ID: -------------------------- Sent from my BlackBerry Wireless Handheld ----- Original Message ----- From: histonet-request Sent: 01/28/2008 06:01 PM GMT To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 50, Issue 39 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Cannabinoid receptor Abs (Carl Hobbs) 2. Kristensen's Sodium Formate/Formic acid recipe (jstaruk) 3. RE: Kristensen's Sodium Formate/Formic acid recipe (Rittman, Barry R) 4. advice about microtomes please! (Patty Dunlop) 5. Motorized vs not RE: [Histonet] advice about microtomes please! (Cheryl) 6. Re: HER2 fixation time (C.M. van der Loos) 7. thanks (louise renton) 8. FW: Lab science gets a new face on Facebook! (Luis Chiriboga) 9. Epstein Barr Virus Ab and Control Tissue (Geraci-Erck, Maria) 10. Camera Lucida (Dr. Catherine L. Ryan) 11. Re: Camera Lucida (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Sun, 27 Jan 2008 18:14:45 -0000 From: "Carl Hobbs" Subject: [Histonet] Cannabinoid receptor Abs To: "Histonet" Message-ID: <001f01c86110$7f76eb10$4001a8c0@carlba65530bda> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Anyone out there use Abs against CB1/2 on frozen/pwax tissues/cells? I would be most grateful for your views. Thanks. Carlos ------------------------------ Message: 2 Date: Sun, 27 Jan 2008 19:04:56 -0500 From: "jstaruk" Subject: [Histonet] Kristensen's Sodium Formate/Formic acid recipe To: "'Histonet'" Message-ID: <0902019EEC4E470892B0FFC176FD6845@JimPC> Content-Type: text/plain; charset="US-ASCII" Does anyone have the recipe and procedure for Kristensen's Sodium Formate/Formic acid for demineralization of teeth? Thank you Jim _____________________ Jim Staruk Mass Histology Service www.masshistology.com ------------------------------ Message: 3 Date: Sun, 27 Jan 2008 20:28:58 -0600 From: "Rittman, Barry R" Subject: RE: [Histonet] Kristensen's Sodium Formate/Formic acid recipe To: "jstaruk" , "Histonet" Message-ID: Content-Type: text/plain; charset="iso-8859-1" 340 gms dried sodium formate 1700 ml. w/v 90% formic acid Distilled water to 10 liters. Fix as usual Rinse in running tap water after formalin fixation. Demineralize using agitation such as electronic stir bar. (Difficult to over deminerlize with this formula). Usually takes 3 to 5 times longer than 5% nitric acid. Wash well in running tap water . Sorry don't have a reference as I am at home but have used this for several years and prefer this to all other demineralization methods except EDTA. Worked well with many IHC techniques. Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of jstaruk Sent: Sun 1/27/2008 6:04 PM To: 'Histonet' Subject: [Histonet] Kristensen's Sodium Formate/Formic acid recipe Does anyone have the recipe and procedure for Kristensen's Sodium Formate/Formic acid for demineralization of teeth? Thank you Jim _____________________ Jim Staruk Mass Histology Service www.masshistology.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Sun, 27 Jan 2008 18:32:39 -0800 From: "Patty Dunlop" Subject: [Histonet] advice about microtomes please! To: histonet@lists.utsouthwestern.edu Message-ID: <80ab7bc60801271832w4113190l143854a9badf532d@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hello again, I appreciate all responses about microtome of choice. I would like to ask for guidance on my specific situation. In my facility, I will be sectioning approximately 25-30 blocks/day of GI (upper and lower) tissue (mostly biopsies and some polyps) and possibly prostate. I have never used a manual microtome and do not know how repetitive it can be. In terms of ergonomics with worries of repetitive motion and carpel tunnel, should I try to convince my boss to get a motorized microtome, or will a manual suffice? I know that motorized microtomes are probably twice as expensive, and although I would feel more comfortable using the same one that I am used to, maybe it would be "going overboard" to get the motorized in my situation. Advice please! Patty ------------------------------ Message: 5 Date: Sun, 27 Jan 2008 21:20:51 -0600 From: "Cheryl" Subject: Motorized vs not RE: [Histonet] advice about microtomes please! To: "'Patty Dunlop'" , Message-ID: <00da01c8615c$c9a728c0$6701a8c0@FSROGER> Content-Type: text/plain; charset="us-ascii" Hi Patti-- While I agree if you can afford one, get a motorized, how much is that carpal surgery and the associated problems vs. the price of a microtome? It isn't the end of the world if you don't get it, though. I've been a tech for 25 years and haven't had this resource available at most of my jobs. In cutting, I can rock n roll over 300 biopsy blocks with levels in a day (yes, good sections but only cutting--not getting up to do anything else). I haven't worked 100% on a histo bench this whole time but in the days of cutting like this every day--it was my SHOULDERS that got tired, not my wrists. If you're prone to carpal and a histotech--you're probably going to get it eventually. But you can slow it down and minimize the risk. (That department is called risk 'management', not risk elimination!!) The bigger issue is sitting high enough & close enough that you aren't holding your shoulders up with your neck muscles and that you alternate activities, stretch, take breaks...etc. The best microtome for your body might be the worst for someone else. Try to get a demo model and TRY IT. Pay attention to any aches or pains because although your body will adjust and get used to the situation--that doesn't mean the damage has stopped occuring. If it doesn't fit you--try the next model! PREVENTION is ALWAYS a better investment than banking on a reasonable cure. Taking care of your body is a big part. Make sure you take enough B vitamins (soft tissue support) drink enough water to support your own system (muscles and connective tissue) and any other supplements a good qualified nutrition/health food consultant might suggest for you. It is amazing how many of us don't drink water because we have to leave the lab to do so! Your state deparment of labor will have an ergonomics division and can send someone out to evalutate your setup to be sure you are maximizing the reduction of risk for repetitive motion and seat position for good health. One of my labs sent me through ergo training (Washington State) and I still use the manuals for setting up workstations in my office and when I travel and work in temp situations. I type 6-8 hours a day at a computer and can be on the phone over 40 hrs a week--same issues--and I'm still going strong (knock on wood!) Best wishes in your search! Cheryl Cheryl R. Kerry, HT(ASCP), BA Full Staff Inc. Staffing the lab - One GREAT tech at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone admin@fullstaff.org www.fullstaff.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patty Dunlop Sent: Sunday, January 27, 2008 8:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] advice about microtomes please! Hello again, I appreciate all responses about microtome of choice. I would like to ask for guidance on my specific situation. In my facility, I will be sectioning approximately 25-30 blocks/day of GI (upper and lower) tissue (mostly biopsies and some polyps) and possibly prostate. I have never used a manual microtome and do not know how repetitive it can be. In terms of ergonomics with worries of repetitive motion and carpel tunnel, should I try to convince my boss to get a motorized microtome, or will a manual suffice? I know that motorized microtomes are probably twice as expensive, and although I would feel more comfortable using the same one that I am used to, maybe it would be "going overboard" to get the motorized in my situation. Advice please! Patty _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Mon, 28 Jan 2008 09:13:47 +0100 From: "C.M. van der Loos" Subject: [Histonet] Re: HER2 fixation time To: histonet@lists.utsouthwestern.edu Cc: Keith.Danielson@uphs.upenn.edu Message-ID: <29bafb295d7c.295d7c29bafb@amc.uva.nl> Content-Type: text/plain; charset=us-ascii Hi, During my workshop in Denver 'how to make a new antibody work for IHC?' I showed the audience a picture taken from the paper of Shi, Liu and Taylor in J Histochem Cytochem 55:105-109, 2007. In this paper there is the result shown of a fixation experiment from 6 hs to 30 days. According to the images presented there is no loss of Her2 within that time frame. Cheers, Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Fri, 25 Jan 2008 12:15:56 -0500 From: "Danielson, Keith" Subject: RE: [Histonet] Re: HER2 fixation time To: "Rene J Buesa" , "Della Speranza, Vinnie" , "Dawson, Glen" Cc: histonet@lists.utsouthwestern.edu Hello, The link below might be of interest. The article cites an interesting study by Arber in 2002 (Arber DA. Effect of prolonged formalin fixation on the immunohistochemical reactivity of breast markers. Appl Immunohistochem Mol Morphol. 2002;10:183-186.). He demonstrated that prolonged fixation of breast tissues in formalin for 7-14 days did not significantly affect immunoreactivity of Her2. I have not been able to get the full article yet -- I would appreciate receiving a PDF of it. http://findarticles.com/p/articles/mi_qa3725/is_200710/ai_n21099762 I am currently growing Her2 expressing cells in cell culture and plan to examine the effect of formalin fixation time and paraffin embedding on immnunoreactivity by IHC. Basically, I am making some control Her2 paraffin blocks for validation purposes. Keith Danielson, PhD Department of Pathology Pennsylvania Hospital Philadelphia, PA 19107 ------------------------------ Message: 7 Date: Mon, 28 Jan 2008 10:49:15 +0200 From: "louise renton" Subject: [Histonet] thanks To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Thanks to all who responded to my question re charging for immunos, much appreciated. haev a good week everyboby! -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ------------------------------ Message: 8 Date: Mon, 28 Jan 2008 07:59:15 -0500 From: "Luis Chiriboga" Subject: [Histonet] FW: Lab science gets a new face on Facebook! To: "IHCRG" , "HISTONET" Message-ID: Content-Type: text/plain; charset=us-ascii -----Original Message----- From: owner-mls-l@ms3.hunter.cuny.edu [mailto:owner-mls-l@ms3.hunter.cuny.edu]On Behalf Of Regina Linder Sent: Friday, January 25, 2008 3:25 PM To: mls-l@hunter.cuny.edu Subject: Fwd: Lab science gets a new face on Facebook! Hello Everyone: As the semester begins, check out this terrific site. "Labs are Vital" is on Facebook, with info on careers in all kinds of labs, scholarship contests, etc. You can download terrific posters to raise consciousness in your hospitals and your kid's schools. All the best, Dr. L If you are unable to see the email clearly, please follow the link below: [ please click here] Labs Are Vital officially joined the Facebook generation in December. In an effort to draw future professionals to clinical lab science, Labs Are Vital has launched a new recruitment campaign on Facebook with information on educational and career opportunities, and a $2,500 scholarship contest. An announcement letter about the Facebook campaign and scholarship opportunities was also mailed to over 10,000 high school counselors and science department heads. Contest entrants may submit a video, advertisement or T-shirt design that encourages others to consider careers in laboratory medicine. Semifinalist submissions will be posted online, where Facebook members will vote on those that most creatively illustrate why clinical lab science is cutting edge and how laboratorians make a difference. Abbott is joined in this effort by ASCLS, APHL and Blood Systems, Inc. Equipment donation call for entries! Labs Are Vital invites all accredited CLS/CLTS programs to apply for our latest round of equipment donations. So far, 43 programs have been selected to receive state-of-the-art diagnostic equipment, supplies and service through the Labs Are Vital $1 million donation program. You'll find winner profiles, and have the opportunity to apply for the next round of donations. But don't wait-applications are due March 31, 2008! For application, click here. The polls have closed and the results are in! Thanks so much to the many of you who participated in our recent Labs Are Vital survey. The response rate was gratifying. While we are still in the process of analyzing the data, the top-line response lets us know that the majority of you have been instrumental in passing the word about Labs Are Vital, which has resulted in many new members. And your suggestions about what you would like us to add will only help improve Labs Are Vital and make it even better in 2008. We'll be holding our drawing for 100 Labs Are Vital lab coats in mid-February, and will announce the winners shortly thereafter. We'll also be sharing more of the survey results with you-the lab professionals who keep health care moving forward. Send a copy of this e-mail to a colleague Unsubscribe If you are unable to see this email clearly, please click here Download Labs Are Vital posters Our Labs Are Vital posters not only celebrate the remarkable contributions of lab professionals, they've also proven to be quite popular with laboratorians and the general public alike. To download one or more of this popular series, click here. Powered by NetDyaLog _________________________________________________________________________ Please address your reply directly to mls-l@hunter.cuny.edu. For help, send a message to majordomo@hunter.cuny.edu with the word "help" in the body of your message. To unsubscribe, send a message to majordomo@hunter.cuny.edu with following command in the body of your message: unsubscribe mls-l your_email_address _________________________________________________________________________ ------------------------------ Message: 9 Date: Mon, 28 Jan 2008 08:02:39 -0500 From: "Geraci-Erck, Maria" Subject: [Histonet] Epstein Barr Virus Ab and Control Tissue To: Message-ID: Content-Type: text/plain; charset="us-ascii" Dear Histonetters, I need to label monkey tissue for Epstein Barr Virus. Does anyone out there have experience with this procedure? I would like to know if there is a particular Ab that would identify an active infection. There are a number of Ab's out there and I was wondering if there is a favorite that gives consistent results. I am also in need of positive control tissue. I have e-mailed Pantomics and unfortunately, they did not have a block. I found a company that sells slides, but in my opinion, they are pricey. Any guidance would be appreciated. Maria Maria Geraci-Erck Schering-Plough Research Institute Special Techniques Laboratory 556 Morris Avenue, Bldg. S-12 Summit, New Jersey 07901-1002 Phone: (908) 473-4284 Fax: (908) 473-4420 maria.geraci-erck@spcorp.com ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. ------------------------------ Message: 10 Date: Mon, 28 Jan 2008 13:25:31 -0400 From: "Dr. Catherine L. Ryan" Subject: [Histonet] Camera Lucida To: histonet@lists.utsouthwestern.edu Message-ID: <479DD7C1.12905.349CDCCF@localhost> Content-Type: text/plain; charset=US-ASCII Hello everyone: I am interested in purchasing a camera lucida attachment for my scope.Does anyone have one they are no longer using and would be interested in selling? Cathy Ryan Dr. Catherine L. Ryan Department of Psychology University of Prince Edward Island Charlottetown, P.E.I., Canada, C1A 4P3 Ph# (902)566-0323/ FAX#(902)628-4359 email: RYAN@UPEI.CA ------------------------------ Message: 11 Date: Mon, 28 Jan 2008 09:36:54 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Camera Lucida To: ryan@upei.ca, histonet@lists.utsouthwestern.edu Message-ID: <27813.7341.qm@web61224.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I have seen one or two in e-Bay Ren? J. "Dr. Catherine L. Ryan" wrote: Hello everyone: I am interested in purchasing a camera lucida attachment for my scope.Does anyone have one they are no longer using and would be interested in selling? Cathy Ryan Dr. Catherine L. Ryan Department of Psychology University of Prince Edward Island Charlottetown, P.E.I., Canada, C1A 4P3 Ph# (902)566-0323/ FAX#(902)628-4359 email: RYAN@UPEI.CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 50, Issue 39 **************************************** ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From liz <@t> premierlab.com Mon Jan 28 12:31:18 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Mon Jan 28 12:31:31 2008 Subject: [Histonet] need help with in-situ hybridization buffer Message-ID: Hello All We need to make up a hybridization buffer for in-situ on paraffin sections, we are using a genedetect probe, I was wondering if there are any commercially available buffers or a simpler one that the one in the Gene Detect protocol. Any advice would be appreciated. Thanks Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 From nyilmaz <@t> mersin.edu.tr Mon Jan 28 13:10:57 2008 From: nyilmaz <@t> mersin.edu.tr (Nejat) Date: Mon Jan 28 13:12:29 2008 Subject: [Histonet] Rat corpus callosum formation Message-ID: <004301c861e1$a2b42530$0401a8c0@NEJAT1> Hi, we have recently started to work on corpus callosum formation in the developing mamalian forebrain. First we performed a pilot study to see the callosol axon projections on E 14 and E 17 mouse foetus brain regarding the reference studies. We could see the callosal axons on the coronal sections as shown in the references . But we decided to run the study with rat foetuses instead of mice because of the lack of the facilities. However we could not find any spesific timing information in the literature about CC formation in rats . We wonder when CC spesificly begins and ends to form during the rat gestation and also where we suppose to see the growing callosal axons on E 14, 15 and 17 on the coronal histological sections in rats. I would greatly appreciate if you could inform me about these issues . Thank you in advance for any information you can suggest me. Yours Sincerely Dr. Necat Yilmaz Mersin Universitesi Tip Fakultesi Histoloji ve Embriyoloji Anabilim Dali From mward <@t> wfubmc.edu Mon Jan 28 13:27:13 2008 From: mward <@t> wfubmc.edu (Martha Ward) Date: Mon Jan 28 13:27:25 2008 Subject: [Histonet] Her2 fixation issue Message-ID: <61135F0455D33347B5AAE209B903A30420789F1F@EXCHVS2.medctr.ad.wfubmc.edu> As is everyone, we are working out the issues of fixation times for samples requiring Her2. How is everyone handling the issue as far as reporting results? Are you making a comment that the fixation was over or under the recommended times and then making a recommendation whether to reflex to FISH? I look forward to hearing how everyone is handling these situations. Thanks, Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center Medical Center Blvd. Winston-Salem, NC 27157 336-716-2756 mward@wfubmc.edu From rjbuesa <@t> yahoo.com Mon Jan 28 14:52:09 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jan 28 14:52:22 2008 Subject: [Histonet] Her2 fixation issue In-Reply-To: <61135F0455D33347B5AAE209B903A30420789F1F@EXCHVS2.medctr.ad.wfubmc.edu> Message-ID: <300815.6569.qm@web61220.mail.yahoo.com> As a rule we used to report IF the CAP protocol was followed or if there were some deviations and which they were. Ren? J. Martha Ward wrote: As is everyone, we are working out the issues of fixation times for samples requiring Her2. How is everyone handling the issue as far as reporting results? Are you making a comment that the fixation was over or under the recommended times and then making a recommendation whether to reflex to FISH? I look forward to hearing how everyone is handling these situations. Thanks, Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center Medical Center Blvd. Winston-Salem, NC 27157 336-716-2756 mward@wfubmc.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From John.McGinley <@t> ColoState.EDU Mon Jan 28 15:46:54 2008 From: John.McGinley <@t> ColoState.EDU (McGinley,John) Date: Mon Jan 28 15:47:07 2008 Subject: [Histonet] Cleaved caspase 3 Message-ID: Hi, Our lab recently purchased cleaved caspase 3 antibody (rabbit polyclonal) from Biocare for marking apoptosis, but the staining intensity is somewhat weak and does not appear to be marking the more classic examples of apoptotic cells that are typically identified via H&E. We tried both LSAB and Dako Envision on FFPE rat mammary tumors, but the staining patterns were similar. We've been using citrate buffer pH 6.0 for HIER, but we were wondering if anyone out there had better experience with other buffers like EDTA or maybe using enzyme pre-treatment in addition or instead of using HIER. Is this the best primary antibody of choice? Would the Cell Signaling antibody perform better on rat tissue? Any input would be greatly appreciated. Thanks in advance. Regards, John McGinley Cancer Prevention Laboratory Colorado State University From slappycraw <@t> yahoo.com Mon Jan 28 17:07:20 2008 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Mon Jan 28 17:07:34 2008 Subject: [Histonet] Cleaved caspase 3 Message-ID: <28887.50852.qm@web53604.mail.re2.yahoo.com> We use the Cell Signaling Ab. with Citrate ph 6.0 and Dako Envision at 1:50 or 1.3ug/ml and it has been pretty good for the most part. Larry A. Woody Seattle, Wa. ----- Original Message ---- From: "McGinley,John" To: "histonet@lists.utsouthwestern.edu" Sent: Monday, January 28, 2008 1:46:54 PM Subject: [Histonet] Cleaved caspase 3 Hi, Our lab recently purchased cleaved caspase 3 antibody (rabbit polyclonal) from Biocare for marking apoptosis, but the staining intensity is somewhat weak and does not appear to be marking the more classic examples of apoptotic cells that are typically identified via H&E. We tried both LSAB and Dako Envision on FFPE rat mammary tumors, but the staining patterns were similar. We've been using citrate buffer pH 6.0 for HIER, but we were wondering if anyone out there had better experience with other buffers like EDTA or maybe using enzyme pre-treatment in addition or instead of using HIER. Is this the best primary antibody of choice? Would the Cell Signaling antibody perform better on rat tissue? Any input would be greatly appreciated. Thanks in advance. Regards, John McGinley Cancer Prevention Laboratory Colorado State University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Never miss a thing. Make Yahoo your home page. http://www.yahoo.com/r/hs From jnocito <@t> satx.rr.com Mon Jan 28 19:00:41 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Jan 28 19:00:39 2008 Subject: [Histonet] PhD in CLS Message-ID: <002f01c86212$5effb990$0302a8c0@yourxhtr8hvc4p> I know that this topic might not affect histology know, but I'm sure it will later. I was just reading the survey results in Advance for Laboratory Medicine and many MTs are for it, surprising as it is. I know that there are many of you with doctorate degrees and I'm wondering how you think this affected your employment, salary and positions. Right now I'm struggling with myself to get a graduate degree. The lazy side of me is saying what will it get me? I'm as far as I can go in my current position. There just isn't any higher positions for a PA working for the military. The rational side says education is never wasted. Just a thought. Wow, I know this is pretty deep for me. See, I bet some of you thought I didn't have a serious side. JTT From akemiat3377 <@t> yahoo.com Mon Jan 28 20:27:43 2008 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Mon Jan 28 20:27:55 2008 Subject: [Histonet] PhD in CLS Message-ID: <320559.34037.qm@web31308.mail.mud.yahoo.com> Hi Joe, Your right, education is never wasted. I remember when Donna Simmons turned 50, she went for her Ph.D. I remember Donna when she worked as an HT in WA State in the early 80's. She later served as a NSH Region 8 director and as a member of our editorial staff. It took her a few years because she was working full time at Hedco Research Institute in LA, while working towards her doctorate. She finally obtained her goal. The last time I spoke with her last year, she was happy with her decision. Then there is David Tacha, who was a HT in research and clinical in the middle 70's. He later got his HTL in the early 80's. He didn't think he would ever need his HTL if he was going to stay in primate research. Pauline Keegan, his mentor, talked him into taking the HTL exam. He never regreted taking his HTL exam. When he moved into Biotech, he required a Ph.D to establish his credibility for Biocare as the head of IHC R&D. He was in his 50's when he got his Ph.D. I'm sure he never has regreted all the hard work obtaining his goal. Akemi Akemi Allison-Tacha BS, HT (ASCP) HTL Client Services Manager PhenoPath Laboratories 551 N 34th St., #100 Seattle, WA 98103 (206) 374-9000 akemi@phenopath.com http://www.phenopath.com --- Joe Nocito wrote: I know that this topic might not affect histology know, but I'm sure it will later. I was just reading the survey results in Advance for Laboratory Medicine and many MTs are for it, surprising as it is. I know that there are many of you with doctorate degrees and I'm wondering how you think this affected your employment, salary and positions. Right now I'm struggling with myself to get a graduate degree. The lazy side of me is saying what will it get me? I'm as far as I can go in my current position. There just isn't any higher positions for a PA working for the military. The rational side says education is never wasted. Just a thought. Wow, I know this is pretty deep for me. See, I bet some of you thought I didn't have a serious side. JTT Akemi Allison-Tacha, BS, HT(ASCP)HTL President Phoenix Lab Consulting & Staffing Tele: (925)788-0900 E-Mail: akemiat3377@yahoo.com From info <@t> hphisto.com Mon Jan 28 22:09:41 2008 From: info <@t> hphisto.com (info@hphisto.com) Date: Mon Jan 28 22:09:56 2008 Subject: [Histonet] Short term wok needed Message-ID: <9017968.760201201579781300.JavaMail.servlet@perfora> Hey histonetters! I am making myself available for immediate short term work. 2-6 weeks. 30 years experience clinical and immunohistochemistry. Anywhere in continental US, with preference of Des Moines area or Prescott/Prescott Valley areas. CV available on request.?Please contact via e-mail at? info@hphisto.com. Bill O'Donnell HT (ASCP) QIHC High Performance Histology Services LLC www.hphisto.com From igor.deyneko <@t> gmail.com Mon Jan 28 23:26:31 2008 From: igor.deyneko <@t> gmail.com (Igor Deyneko) Date: Mon Jan 28 23:26:42 2008 Subject: [Histonet] Aqueous Mounting Medium in In-Situ Message-ID: <35e16a770801282126r36626035i598f8402f8805f7@mail.gmail.com> Dear Histonetters! I was wondering if anyone can help me with this question. I will be trying some In-Situ Hybridization and the protocol suggest covering the slides using thick Glycergel. i gave it a try today and get a lot of bubbles and overall it's hard to coverslip. I was wondering if anyone has ever used Aqueous Mounting Media to coverslip In-Situ slides? I used it for fluorescence and then sealed off the corners with clear nail polish. Thank you for any suggestions. Igor Deyneko Infinity Pharmaceuticals Cambridge, MA From louise.renton <@t> gmail.com Tue Jan 29 01:44:38 2008 From: louise.renton <@t> gmail.com (louise renton) Date: Tue Jan 29 01:44:49 2008 Subject: [Histonet] killing & resin embedding scorpions (ot) Message-ID: hi all, this is a slightly off topic query. What is the best way to euthanize a scorpion, and if t is to be embedded i resin, should I open it up for processing? (No sections to be cut) -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue Jan 29 02:12:08 2008 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Jan 29 02:12:23 2008 Subject: [Histonet] PhD in CLS Message-ID: <86ADE4EB583CE64799A9924684A0FBBF03CAE080@wahtntex2.waht.swest.nhs.uk> Don't think anyone ever was turned down for a job because they had a PhD but many have who haven't. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Don't be afraid to take a big step when one is indicated. You can't cross a chasm in two small jumps. --Buckminster Fuller This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From carl.hobbs <@t> kcl.ac.uk Tue Jan 29 03:02:52 2008 From: carl.hobbs <@t> kcl.ac.uk (carl hobbs) Date: Tue Jan 29 03:03:39 2008 Subject: [Histonet] Re: PECAM-1 antibody Message-ID: <002301c86255$baa42180$112b5c9f@kclfrgvhctwh15> Many thanks for your comments/advice. I would like to use two Abs and it seems that anti CD34 and anti CD31 should be the best pair. Carl Hobbs Histology Manager Wolfson Centre for Age-Related Diseases Wolfson Wing Hodgkin Building Guys Campus Kings College London London SE1 1UL 020 78486810 London From nefff <@t> staff.uni-marburg.de Tue Jan 29 08:56:16 2008 From: nefff <@t> staff.uni-marburg.de (Dr. med. Frauke Neff) Date: Tue Jan 29 08:56:28 2008 Subject: [Histonet] negative control Message-ID: <1201618576.479f3e9020c51@webmail.med.uni-marburg.de> Hello! I've got a limited amount of slides and want to stain a negative control that is already stained as a negative control with the MOM-Kit from Vectastain using DAB as chromogen. This slide displayes no background. Now I want to use this slide as a negative control with the ABC-Kit from Vectastain and I expect lots of background staining. Do you think this is possible or do you think the aready biotinylated secondary AB of the MOM kit interferes with the anti-human antibody of the ABC kit. I would be happy about any kind of information regarding my problem. Kind regards, F. ---------------------------------------------------------------- This message was sent using IMP, the Internet Messaging Program. From Joyce.Rush <@t> sjmcmn.org Tue Jan 29 10:15:13 2008 From: Joyce.Rush <@t> sjmcmn.org (Rush, Joyce) Date: Tue Jan 29 10:15:57 2008 Subject: [Histonet] CMS PQRI Message-ID: <2337362E8548BE4C85EE5DD5D526B0850122C105@sjw3smail2.SJMCMN.ORG> I've been a bit out of touch with the list and hope that this hasn't been brought up and discussed before. Have any of you begun to participate in the CMS PQRI plan? If so, I'd be interested in knowing how you set it up. Thanks, Joyce Joyce A. Rush, BS, MT(ASCP) Director, Laboratory Services St. Joseph Medical Center 523 N. Third Street Brainerd, MN 56401 Phone: 218-828-7505 FAX: 218-828-7510 This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From gayle.callis <@t> bresnan.net Tue Jan 29 10:24:50 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Tue Jan 29 10:25:36 2008 Subject: [Histonet] PhD in CLS References: <86ADE4EB583CE64799A9924684A0FBBF03CAE080@wahtntex2.waht.swest.nhs.uk> Message-ID: <000d01c86293$7abf4890$6501a8c0@DHXTS541> Interesting, but I do know of a gentleman who had a PhD in plant pathology. He wanted to have a simple outside laborer/day job in arborcare to fill in time while waiting for his wife to finish up her medical degree. When he applied to several tree cutting/maintenance services, the business owners apparently were threatened by his PhD degree, said he was overqualified, and did not hire him. After that, he left his PhD information off job applications, was hired, and later formed his own highly successful profitable arborcare business. Maybe in some ways, he had sweet revenge as competition to "threatened" scaredy cats. Gayle M. Callis HT/HTL/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Kemlo Rogerson" To: "Akemi Allison-Tacha" ; Sent: Tuesday, January 29, 2008 1:12 AM Subject: RE: [Histonet] PhD in CLS Don't think anyone ever was turned down for a job because they had a PhD but many have who haven't. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; From gayle.callis <@t> bresnan.net Tue Jan 29 10:33:27 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Tue Jan 29 10:33:33 2008 Subject: [Histonet] Aqueous Mounting Medium in In-Situ References: <35e16a770801282126r36626035i598f8402f8805f7@mail.gmail.com> Message-ID: <001b01c86294$ad5a5780$6501a8c0@DHXTS541> Igor, Since you are doing fluorescent ISH, I suggest using a hard set aqueous mounting media that helps prevent fading of the fluorophore. Molecular Probes Prolong Gold (excellent quality) is our preference and you can seal with nail polish. We have had the best luck with this particular mounting media even though there are others on the market, maybe you can Google to find more. We have experienced fading of fluorophores with some of the other medias. Also go to IHCWorld website and check out the discussion on autofluorescence/fluorescence - mounting medias are discussed there too. Gayle M. Callis HT/HTL/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Igor Deyneko" To: Sent: Monday, January 28, 2008 10:26 PM Subject: [Histonet] Aqueous Mounting Medium in In-Situ > Dear Histonetters! > I was wondering if anyone can help me with this question. I will be trying > some In-Situ Hybridization and the protocol suggest covering the slides > using thick Glycergel. i gave it a try today and get a lot of bubbles and > overall it's hard to coverslip. I was wondering if anyone has ever used > Aqueous Mounting Media to coverslip In-Situ slides? I used it for > fluorescence and then sealed off the corners with clear nail polish. > Thank you for any suggestions. > Igor Deyneko > Infinity Pharmaceuticals > Cambridge, MA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From octavio109 <@t> hotmail.com Tue Jan 29 11:11:31 2008 From: octavio109 <@t> hotmail.com (Corinthia D. Emanuel) Date: Tue Jan 29 11:11:44 2008 Subject: [Histonet] Full-time employment ATLANTA with sign-on bonus; Histology Lab Manager Message-ID: EMPLOYMENT OPPORTUNITY in ATLANTA, GA SIGNING BONUS OFFERED ASCP-certified Histologist with lab supervision experience ASCP Certified Histologist with 5+ years of supervisor experience, under the supervision of a pathologist or medical scientist, required for a physician-owned pathology laboratory in Atlanta, GA. You must have previous experience in all aspects of specimen processing, such as cutting, grossing, embedding, special stains and immunochemistry Taking charge, you will be responsible for running all aspects of this fast-paced and challenging Histology laboratory. In this management position, you will be expected to develop the workforce skill level, organize work rotations for two shifts, plan capital purchases, maintain consumables stocks, develop QA, QC and EQA policies and procedures, comply with all waste disposal & recordkeeping regulations. In addition, you will develop and maintain all policies and procedures and prepare for all inspections. Some weekend hours may be necessary depending on workflow and staffing needs. This position requires above average communication skills, strong interpersonal skills, along with the character, work ethics and skills needed to lead by example. You must be able to connect and learn from your work force as well as ensure a disciplined workforce. Your computer skills will include Microsoft Word, Excel and experience with laboratory management systems (LMS) will be an advantage. If you are committed to the patient, can keep calm in times of stress and enjoy problem solving and delivering high-level service, we are looking for you. Salary is based on experience. Benefits are available. Please fax a letter of introduction with salary requirements and resume to 770-381-6451, attention C. Emanuel. Or, you may email to ndpsearch@gmail.com. _________________________________________________________________ Shed those extra pounds with MSN and The Biggest Loser! http://biggestloser.msn.com/ From rfields <@t> gidocs.net Tue Jan 29 13:45:28 2008 From: rfields <@t> gidocs.net (Rosa Fields) Date: Tue Jan 29 13:45:48 2008 Subject: [Histonet] glass coverslipper? Message-ID: <2F2611250DCD6549AA3D96CE8AF1F018DAD593@giexchange.gidocs.net> Wondering what experience anyone has had with "used" or refurb. glass coverslippers? I am trying to justify 17 thousand dollar difference, looking at the Leica CV5030. Thanks! Rosa Fields, HT (ASCP) Histology Supervisor Gastroenterology Specialties P.C. 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net From victoria.spoon <@t> bassett.org Tue Jan 29 14:38:31 2008 From: victoria.spoon <@t> bassett.org (Spoon, Victoria) Date: Tue Jan 29 14:41:27 2008 Subject: [Histonet] Senior histotechnician position Message-ID: <415700FC732DE14491A3E39367834F77012458B7@ex3.bassett.org> A Senior histotechnician position is available at Bassett healthcare in Cooperstown, NY. Hours are Monday through Friday 7am to 3:30pm Responsibilities: * Provides prompt, courteous, and accurate laboratory test results to providers and internal departments * Oversees technical staff members * Oversees and coordinates preparation of samples for examination and performance of testing following established procedures * Correlates knowledge and understanding of theory and technical elements of testing to solve problems and answers questions effectively and efficiently Requirements: * Associate's degree * Must be qualified as a Histotechnician in accordance to New York State Department of Health regulations * Two years of previous experience in histology * Licensure as a Clinical Laboratory Technician by NYS SED required. Bassett Healthcare, a magnet designated network of four hospitals and 20+ health centers, offers the finest in medical care to the residents of rural, Central New York. We provide primary and specialty care services to rural communities spanning eight counties. Bassett has evolved from a community hospital to a nationally renowned academic referral center, which employs almost 3,000 in its research and teaching system, and is affiliated with Columbia University. Bassett Healthcare is located in Cooperstown New York, a picturesque lakeside village that offers year round cultural and recreational opportunities and excellent schools. Cooperstown is a great place to raise your family, make friends, and enjoy a superb quality of life! Contact Melanie Craig at (607)547-3120 or apply on line at http://www.bassett.org/careercenter/index.cfm NOTICE OF CONFIDENTIALITY This electronic message, including attachments, is for the sole use of the named recipient and may contain confidential or privileged information protected by New York State, and Federal regulations. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. If you are not the intended recipient or have received this communication in error please contact the sender or email.security@bassett.org and destroy all copies of the original message. Thank you. From contact <@t> excaliburpathology.com Tue Jan 29 14:56:21 2008 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Tue Jan 29 14:56:35 2008 Subject: [Histonet] glass coverslipper Message-ID: <719308.20886.qm@web50111.mail.re2.yahoo.com> I bought my Hacker glass coverslipper on eBay for $400. It works like a charm and even came with the slide racks. Now if someone would just make one for the big 50x75mm slides! Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-759-3953 contact@excaliburpathology.com www.excaliburpathology.com From Nikki_Prpic <@t> bd.com Tue Jan 29 15:02:21 2008 From: Nikki_Prpic <@t> bd.com (Nikki_Prpic@bd.com) Date: Tue Jan 29 15:03:05 2008 Subject: [Histonet] Nikki Prpic is out of the office. Message-ID: I will be out of the office starting 01/29/2008 and will not return until 01/31/2008. I will have limited access to email during this time. I will respond to your email upon my return. If you require immediate assistance, please call me at 919-451-7518. ----------------------------------------- ******************************************************************* IMPORTANT MESSAGE FOR RECIPIENTS IN THE U.S.A.: This message may constitute an advertisement of a BD group's products or services or a solicitation of interest in them. If this is such a message and you would like to opt out of receiving future advertisements or solicitations from this BD group, please forward this e-mail to optoutbygroup@bd.com. ******************************************************************* This message (which includes any attachments) is intended only for the designated recipient(s). It may contain confidential or proprietary information and may be subject to the attorney-client privilege or other confidentiality protections. If you are not a designated recipient, you may not review, use, copy or distribute this message. If you received this in error, please notify the sender by reply e-mail and delete this message. Thank you. ******************************************************************* Corporate Headquarters Mailing Address: BD (Becton, Dickinson and Company) 1 Becton Drive Franklin Lakes, NJ 07417 U.S.A. ******************************************************************* From LINDA.MARGRAF <@t> childrens.com Tue Jan 29 15:36:57 2008 From: LINDA.MARGRAF <@t> childrens.com (LINDA MARGRAF) Date: Tue Jan 29 15:37:27 2008 Subject: [Histonet] Job in Virginia References: <479F0208020000DA0001DCE6@CNET3.CHILDRENS.COM> <479F4819020000DA0001DDBE@CNET3.CHILDRENS.COM> Message-ID: <479F4819020000DA0001DDBE@CNET3.CHILDRENS.COM> Here's a job one of the Histonet members was having trouble posting.... LAB SECTION SPECIALIST Req. #: 08-38409 Facility: Inova Fair Oaks Hospital Location: Fairfax, VA US Department: Histology Work Schedule: Full Time, M-F Shift: Day Job Level: Education: Category: Laboratory Position Summary: Directs the daily technical operations of the section, maintaining quality standards and procedures in order to meet CAP, JCAHO, AABB, CLIA and other regulatory agency requirements. Designs employee schedules and assignments to ensure the proper and timely completion of work, the health and safety of employees and the overall efficient operation of the section. Participates in routine testing procedures, required maintenance and repair, instrument calibration and result verification. Requirements: Recent consecutive experience in specialty to include the demonstrated abilities in teaching and/or human resource development and to communicate and problem solve. NAACLS program completed or experience as required. Medical Lab Tech (American Society of Clinical Pathologist - ASCP) or equivalent. Familiar with computer and computerized equipment, organization and team skills required. Education Requirements: BS in Science. https://inova.hrdpt.com/cgi-bin/a/highlightjob.cgi?jobid=99998615 Above is the link. Click on it, then click on the button "Apply for this position." Interested applicants could also contact the laboratory supervisor, Mary Munchak, via email at Mary.Munchak@inova.org if they want more information before entering the HR application link. Thanks From vetdrrkc <@t> gmail.com Tue Jan 29 16:28:50 2008 From: vetdrrkc <@t> gmail.com (Ratan K) Date: Tue Jan 29 16:29:07 2008 Subject: [Histonet] regarding quantum dots protocol Message-ID: Hi Histonetters, I tried to label BrdU and estrogen receptor antigen with qdots labeled secondary antibody, but failed to get satisfactory result. I am interested to know that is anyone using qdots protocol if so, please help me in sending protocol. thanks ratan -- http://connexions.rediff.com/connexions/Main.php?do=profile&id=0&qid=1782800 From danand24 <@t> gmail.com Wed Jan 30 07:31:49 2008 From: danand24 <@t> gmail.com (Anand D) Date: Wed Jan 30 07:32:01 2008 Subject: [Histonet] Old tissue for doublecortin staining Message-ID: <30281590801300531o49b87b86x23a651922373d967@mail.gmail.com> Dear Friends, I need your help for the following situation. In my new lab, they have mouse brain tissues stored in PBS (4 degree c) for the past one year. Now my task is to do doublecortin immunostaining in these tissues. I tried several time (without antigen retrieval method) but I could not able to get any staining. However, when I tried with GFAP I could able to see some staining. Can anybody help suggest any methods by which I can get doublecortin staining in these old tissues? I appreciate your help in advance, Thank you Anandh From sheila_adey <@t> hotmail.com Wed Jan 30 07:53:14 2008 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Wed Jan 30 07:53:26 2008 Subject: [Histonet] Cytology prep question Message-ID: Hi netters, Just wondering how other hospitals are setting up their pleural fluids and bronchs. Do you spin down your specimen and then use the same tube for your cell block and slides or do you spin down equal amounts separately and use one for the slides and one for the block? Any input is greatly appreciated.Sheila Adey HT MLTPort Huron HospitalMichigan _________________________________________________________________ From portera <@t> msu.edu Wed Jan 30 08:50:07 2008 From: portera <@t> msu.edu (Amy Porter) Date: Wed Jan 30 08:56:25 2008 Subject: [Histonet] Source for slides of vaginosis wet preps Message-ID: <000801c8634f$67da8700$8e7a0923@histolab> If anyone knows of a source that wet preps for bacterial vaginosis can be purchased, I would greatly appreciate that information. Our nursing college now needs to have review of these slides added to their curriculum for nursing students and is in search of somewhere to purchase these. Their deadline is February 7th. Thanks in advance - Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 355-6475 ext 1480 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu From Valerie.Hannen <@t> parrishmed.com Wed Jan 30 09:34:10 2008 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Wed Jan 30 09:34:24 2008 Subject: [Histonet] Recyclers Message-ID: <5680DA93771F0C48954CC8D38425E72401AB34C3@ISMAIL.parrishmed.local> Hi folks, I was wondering if anyone else uses the Chemical Recycler from CBG Biotech? We use it for Alcohol, Xylenes and Zinc Formalin. The Alcohol and Xylenes come out great, however our Zinc Formalin comes out suboptimal. It does not fix as well or fast as the product that we purchase new. We sent a sample to CBG to be analysed, and their reply was to say that it was more pure than the purchased product. Does "more pure" mean as good as or better? We realize that the recycling takes out the Zinc, so we do replace it with a Zinc concentrate supplied by the company. We have (Histology) conducted our own "experiments" using tissues placed in the recycled product with the Zinc concentrate added and tissues placed in the recycled product with out the Zinc Concentrate. No real difference noted. Any insight that can be shared would be greatly appreciated. Valerie Hannen, MLT(ASCP),HTL,SU(FL) Histotechnologist Parrish Medical Center Titusville,Florida (321)268-6333 Ext.7506 ************************************************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately notify us by telephone at 321-268-6167 and return the original message to us at the listed email address. Thank you From bdornier <@t> gmail.com Wed Jan 30 09:48:40 2008 From: bdornier <@t> gmail.com (Brandon Dornier) Date: Wed Jan 30 09:48:55 2008 Subject: [Histonet] In Situ Hybridization Blue Haze Problems Message-ID: <8556a07e0801300748g458bc254wbe3d90e553cec3b8@mail.gmail.com> I have an In Situ Hybridization question for you guys and gals. I'm currently performing In Situ HPV and EBV tests using an automated machine called the XT by Ventana. The tissue is parrifin embedded, cut at 4 microns and place on charged slides. I am beginning to notice some background staining that varies in intensity. When it occurs on our QC slide, it can be strong enough to fail a run. The background resembles a "blue haze" that can cover the tissue making interpretation difficult. At other times the blue haze surrounds the tissue rather than covering it which makes me believe that there could be some sort of hydrophobic or other chemical rxn taking place to shield the tissue. I'm not certain if these two problems are related, but both occur completely random and always vary in intensity. If I can find the culprit of this problem it would lead to cleaner slides and more conclusive resulting. If anybody can help it would be much appreciated. Thanks From bdornier <@t> gmail.com Wed Jan 30 10:13:29 2008 From: bdornier <@t> gmail.com (Brandon Dornier) Date: Wed Jan 30 10:13:42 2008 Subject: [Histonet] In Situ Hybridization Blue Background problem Message-ID: <8556a07e0801300813o67fe5e3cnbb6e598124bebf00@mail.gmail.com> I have an In Situ Hybridization question for you guys and gals. I'm currently performing In Situ HPV and EBV tests using an automated machine called the XT by Ventana. The tissue is parrifin embedded, cut at 4 microns and place on charged slides. NBT/ BCIP is used as our chromogen. I am beginning to notice some background staining that varies in intensity. When it occurs on our QC slide, it can be strong enough to fail a run. The background resembles a "blue haze" that can cover the tissue making interpretation difficult. At other times the blue haze surrounds the tissue rather than covering it which makes me believe that there could be some sort of hydrophobic or other chemical rxn taking place to shield the tissue. I'm not certain if these two problems are related, but both occur completely random and always vary in intensity. If I can find the culprit of this problem it would lead to cleaner slides and more conclusive resulting. If anybody can help it would be much appreciated. Thanks P.S. Ventana has been notified of the problem and engineers have been sent multiple times to try to figure it out. From talulahgosh <@t> gmail.com Wed Jan 30 10:31:51 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Wed Jan 30 10:32:04 2008 Subject: [Histonet] In Situ Hybridization Blue Haze Problems In-Reply-To: <8556a07e0801300748g458bc254wbe3d90e553cec3b8@mail.gmail.com> References: <8556a07e0801300748g458bc254wbe3d90e553cec3b8@mail.gmail.com> Message-ID: When we've had that problem, it was due to expired or precipitated NBT and/or BCIP. Emily -- fortune smiles on the brave and spits on the coward --aguirre, wrath of god From SDrew <@t> uwhealth.org Wed Jan 30 11:13:20 2008 From: SDrew <@t> uwhealth.org (Drew Sally A.) Date: Wed Jan 30 11:14:19 2008 Subject: [Histonet] Antibody query Message-ID: <3F328377AF4E23438E78234752652CE105D52618@uwhis-xchng7.uwhis.hosp.wisc.edu> We are wondering what vendors people are using for the following antibodies; MLH-1, MSH-2, MSH-6 and PMS-2. Anyone running these on the Ventana immunostainers? Thank you for any and all input! Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 From tanisha.mcknight <@t> covance.com Wed Jan 30 11:58:53 2008 From: tanisha.mcknight <@t> covance.com (McKnight, Tanisha) Date: Wed Jan 30 11:59:06 2008 Subject: [Histonet] Slide Trays for Refrigeration of Paraffin Dipped Slides Message-ID: <816E3C72F855F14985FC31D7C963AE6F0427F237@indexch03.ent.covance.com> Hello All: I was wondering if anyone can recommend a slide storage system that works well (safe, maximum use of space) in refrigerators. I will be storing a large amount of paraffin dipped slides in refrigerators and I am looking for something that slides in and out easily, but safely. I also want something that will give me the best use of my space. Thanks in advance for any suggestions. Tanisha Neely, HT (ASCP) ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From tanisha.mcknight <@t> covance.com Wed Jan 30 12:05:53 2008 From: tanisha.mcknight <@t> covance.com (McKnight, Tanisha) Date: Wed Jan 30 12:06:05 2008 Subject: [Histonet] Slide Trays for Refrigeration of Paraffin Dipped Slides Message-ID: <816E3C72F855F14985FC31D7C963AE6F0427F248@indexch03.ent.covance.com> Hello All: I was wondering if anyone can recommend a slide storage system that works well (safe, maximum use of space) in refrigerators. I will be storing a large amount of paraffin dipped slides in refrigerators and I am looking for something that slides in and out easily, but safely. I also want something that will give me the best use of my space. Thanks in advance for any suggestions. Tanisha Neely, HT (ASCP) ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From Jerry <@t> ralambusa.com Wed Jan 30 12:35:09 2008 From: Jerry <@t> ralambusa.com (Jerry Helisek) Date: Wed Jan 30 12:36:38 2008 Subject: [Histonet] RE: Slide Trays for Refrigeration of Paraffin Dipped Slides Message-ID: <3855F92002259948A66A8CA2D16E3A4F05A974@server.ralambusa.com> How many slides are you storing? Would the slides be touching or seperated? Does Horizonal or vertical matter? We may be able to help, depending on your reply Thanks. Sincerely, ------------------------------------------ Jerry Helisek Raymond A. Lamb, Inc jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------------ ------------------------------ Message: 15 Date: Wed, 30 Jan 2008 12:58:53 -0500 From: "McKnight, Tanisha" Subject: [Histonet] Slide Trays for Refrigeration of Paraffin Dipped Slides To: histonet@lists.utsouthwestern.edu Message-ID: <816E3C72F855F14985FC31D7C963AE6F0427F237@indexch03.ent.covance.com> Content-Type: text/plain Hello All: I was wondering if anyone can recommend a slide storage system that works well (safe, maximum use of space) in refrigerators. I will be storing a large amount of paraffin dipped slides in refrigerators and I am looking for something that slides in and out easily, but safely. I also want something that will give me the best use of my space. Thanks in advance for any suggestions. Tanisha Neely, HT (ASCP) ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 50, Issue 41 **************************************** From info <@t> hphisto.com Wed Jan 30 12:48:06 2008 From: info <@t> hphisto.com (info@hphisto.com) Date: Wed Jan 30 12:48:21 2008 Subject: [Histonet] Temp work needed Message-ID: <11379112.1068651201718884208.JavaMail.servlet@perfora> Mother ill. Would like to find short-term employment in or within commute of Prescott/Prescott Valley, AZ. 30 yrs experience. - Bill O'Donnell HT (ASCP) QIHC High Performance Histology Services LLC www.hphisto.com From cmiller <@t> physlab.com Wed Jan 30 12:48:55 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Wed Jan 30 12:48:38 2008 Subject: [Histonet] FW: Fungas,GMS.Aspergillus Message-ID: <000001c86370$c42eaf60$3d02a8c0@plab.local> Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 _____ From: Cheri Miller [mailto:cmiller@physlab.com] Sent: Wednesday, January 30, 2008 10:58 AM To: histonet-bounces@lists.utsouthwestern.edu Subject: Fungas,GMS.Aspergillus I am in desperate need of control tissue or slides. My supplier is backordered. A long time ago I remember reading that you can grow your own so to speak. Does anyone have any info on this? And was it successful?? Our Microbiology department is game for an experiment, Thanks, Cheri Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From jonesly <@t> mir.wustl.edu Wed Jan 30 12:53:13 2008 From: jonesly <@t> mir.wustl.edu (Jones, Lynne) Date: Wed Jan 30 12:53:25 2008 Subject: [Histonet] Help finding a rat Fas antibody similar to Jo2 for mouse In-Reply-To: <22798b1d-463c-4fcc-97e4-eb3a1fceac69> References: <22798b1d-463c-4fcc-97e4-eb3a1fceac69> Message-ID: Hello - We are hoping to locate an antibody for use in rats similar to the Jo2 hamster anti-mouse Fas antibody. I checked my usual online databases and can find anti-Fas antibodies produced in rats, but none that claim to react with rats. Is anyone aware of such a product? Based on a published papers, Jo2 is specifically non-reactive with rats, as are several other monoclonal and polyclonal Abs. (The PI did an exhaustive search about 5 years ago, but I'm hoping there may be something more recent.) This would be used for both intratracheal instillation into the rat lung, and possibly for IHC on rat tissues following different models for cell death/injury. I would greatly appreciate any advice or assistance from the collective wisdom of HistoNet. Thanks, Lynne Jones Lab Manager Radiological Sciences Washington University School of Medicine Saint Louis, Missouri The materials in this message are private and may contain Protected Healthcare Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return mail. From tanisha.mcknight <@t> covance.com Wed Jan 30 13:45:08 2008 From: tanisha.mcknight <@t> covance.com (McKnight, Tanisha) Date: Wed Jan 30 13:45:23 2008 Subject: [Histonet] RE: Refrigerated slide storage Message-ID: <816E3C72F855F14985FC31D7C963AE6F0427F317@indexch03.ent.covance.com> There are two large refrigerators dedicated to this only. In our original set up we were looking at a capacity of about 24,000 slides per refrigerator. We would like to stay at or exceed this capacity. Vertical or horizontal doesn't matter. What matters most is maximizing capacity, while keeping these slides SUPER SAFE. I am told these are very precious to our client. _____ From: Jerry Helisek [mailto:Jerry@ralambusa.com] Sent: Wednesday, January 30, 2008 1:34 PM To: McKnight, Tanisha Subject: Refrigerated slide storage How many slides are you storing? Would the slides be touching or seperated? Does Horizonal or vertical matter? We may be able to help, depending on your reply Thanks. ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek VP North America jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From funderwood <@t> mcohio.org Wed Jan 30 14:54:51 2008 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Wed Jan 30 14:57:59 2008 Subject: [Histonet] Acid Fast Bacteria/ Ziehl Neelsen In-Reply-To: <337469.9999.qm@web61219.mail.yahoo.com> References: <4799EAB9.EB3B.00B9.0@cvm.tamu.edu> <337469.9999.qm@web61219.mail.yahoo.com> Message-ID: <47A09EE9.BE64.0034.0@mcohio.org> I know that storing in 10% NBF will. I have a bottle full of positive lung that lit up when stained with AFB. After a year or so in formalin, I get no staining what so ever. >>> Rene J Buesa 1/25/2008 3:41 PM >>> Theoretically it might. Remember that serous bacteria covering could be partially dissolved by EthOL. But try it, and let everybody know how it came about. Ren? J Lin Bustamante wrote: Can long storage of wet tissue (over 6 months) in 70 % Etoh diminish the reaction for positive infected tissue with Mycobacterium tuberculosis? Thank you. Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab. Supervisor Dept. of Veterinary Integrative Biosciences Texas A&M University College Station, TX 77843-4458 (979)845-3177 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Wed Jan 30 15:33:08 2008 From: tkngflght <@t> yahoo.com (Cheryl) Date: Wed Jan 30 15:33:28 2008 Subject: [Histonet] A really cool position in Washington State-- In-Reply-To: References: <4FE7FB862E90E448AE32388E759220E50861A5@pbrcas31.pbrc.edu> Message-ID: <008301c86387$b5685eb0$6701a8c0@FSROGER> Hello everyone!! We have a lot of open positions right now and our "Help for Histotechs" mailer regarding continuing education and a few other things went out around Christmas--they're still hitting the mail so please let me know what you think of it! If you haven't filled it out yet, please do... If you want to receive a copy--please reply to this email. We're looking for good candidates for a truly wonderful opportunity. It's a day job, weekdays, with routine histo and lots of interesting other projects through the week. It's in Washington State and we can tell you more if you're at all interested. We're zero-pressure: it's your life-your choice. It's our job to help you find a job where you are happy!! Looking forward to any and all inquiries~ Cheryl Cheryl R. Kerry, HT(ASCP), BA Full Staff Inc. Staffing the lab - One GREAT tech at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone admin@fullstaff.org www.fullstaff.org From rfields <@t> gidocs.net Wed Jan 30 16:42:31 2008 From: rfields <@t> gidocs.net (Rosa Fields) Date: Wed Jan 30 16:42:42 2008 Subject: [Histonet] Thanks! Message-ID: <2F2611250DCD6549AA3D96CE8AF1F018DAD59A@giexchange.gidocs.net> Thanks for all the replies to my coverslipping question! Rosa Fields, HT (ASCP) Histology Supervisor Gastroenterology Specialties P.C. 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net From sccrshlly <@t> yahoo.com Wed Jan 30 19:13:01 2008 From: sccrshlly <@t> yahoo.com (Shelly Coker) Date: Wed Jan 30 19:13:13 2008 Subject: [Histonet] New book for my library??? Message-ID: <715969.77537.qm@web90308.mail.mud.yahoo.com> Hello all, I am considering adding a new text to my library: Theory and Practice of Histological Techniques by John D. Bancroft and Marilyn Gamble. Does anyone own this book? And if so, what do you think of it? The biggest reason I am hesitating is that the book is close to $200 and if it really isn't useful, I would rather not spend the money! Thanks, Shelly --------------------------------- Never miss a thing. Make Yahoo your homepage. From san.htin <@t> yahoo.com Wed Jan 30 19:26:23 2008 From: san.htin <@t> yahoo.com (San Tin) Date: Wed Jan 30 19:26:34 2008 Subject: [Histonet] ISH double staining Message-ID: <958077.15969.qm@web55812.mail.re3.yahoo.com> Hi all, Any one out there with experience of ISH double staining with two different probes? Do you think it is possible? San ____________________________________________________________________________________ Never miss a thing. Make Yahoo your home page. http://www.yahoo.com/r/hs From anthony <@t> histotechexchange.com Wed Jan 30 20:52:55 2008 From: anthony <@t> histotechexchange.com (anthony@histotechexchange.com) Date: Wed Jan 30 21:03:42 2008 Subject: [Histonet] Histotech Manager, By state Requirements. In-Reply-To: <479F4819020000DA0001DDBE@CNET3.CHILDRENS.COM> References: <479F0208020000DA0001DCE6@CNET3.CHILDRENS.COM> <479F4819020000DA0001DDBE@CNET3.CHILDRENS.COM> Message-ID: <1525.65.40.219.240.1201747975.squirrel@host7.wfdns.com> Dear All: I am trying to make a comprehensive list of all of the state requirements for Histotech Lab Managers and for Histotechs, as with NY State. I will be making my research freely available on the internet AND will give it to anyone who asks. Yours truly, Anthony Williams HT (ASCP) Histotech Exchange LLC 19 Whitmore Street Lexington, VA 24450 T 1 877 464 8911 F 1 540 301 0071 anthony@histotechexchange.com www.histotechexchange.com From histology.bc <@t> shaw.ca Wed Jan 30 23:36:03 2008 From: histology.bc <@t> shaw.ca (Paul Bradbury) Date: Wed Jan 30 23:33:52 2008 Subject: [Histonet] New book for my library??? In-Reply-To: <715969.77537.qm@web90308.mail.mud.yahoo.com> References: <715969.77537.qm@web90308.mail.mud.yahoo.com> Message-ID: <47A15E43.3030201@shaw.ca> Hi Shelly, I have been one of the contributing authors to this text since the first edition came out in the late 70's. It was originally put together by John Bancroft and Alan Stevens, plus a host of other histologists when we all still lived in England. Many of the original chapters and their content were discussed in the pubs in and around Nottingham and required many pints of beer before the final drafts were produced. In my opinion, and I may be somewhat biased, it is an excellent text and, as the title suggests, effectively balances the theoretical aspects with sound, proven practical procedures, unlike other texts which either are "recipe manuals" or pure theory. The authors are all well respected histologists or pathologists with vast amounts of experience in their fields. Fortunately, as one of the authors, I get free copies, but I must admit $200 is a lot of money for a book. If your lab can buy it on your behalf, then without a doubt, get it. If it is your own hard-earned cash, I think I would still be very tempted to get it. I don't think you will regret your purchase. Paul Bradbury Kamloops, Canada Shelly Coker wrote: > Hello all, > > I am considering adding a new text to my library: Theory and Practice of Histological Techniques by John D. Bancroft and Marilyn Gamble. Does anyone own this book? And if so, what do you think of it? The biggest reason I am hesitating is that the book is close to $200 and if it really isn't useful, I would rather not spend the money! > > Thanks, > Shelly > > > --------------------------------- > Never miss a thing. Make Yahoo your homepage. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From gu.lang <@t> gmx.at Thu Jan 31 02:37:29 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Jan 31 02:37:45 2008 Subject: AW: [Histonet] New book for my library??? In-Reply-To: <715969.77537.qm@web90308.mail.mud.yahoo.com> Message-ID: <002f01c863e4$8493ef40$eeeea8c0@dielangs.at> I also recommend the book, because it is of both theoretical and practical value. To the editors I have also the wish, that there would be a paperback-edition for the small moneybag. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Shelly Coker Gesendet: Donnerstag, 31. J?nner 2008 02:13 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] New book for my library??? Hello all, I am considering adding a new text to my library: Theory and Practice of Histological Techniques by John D. Bancroft and Marilyn Gamble. Does anyone own this book? And if so, what do you think of it? The biggest reason I am hesitating is that the book is close to $200 and if it really isn't useful, I would rather not spend the money! Thanks, Shelly --------------------------------- Never miss a thing. Make Yahoo your homepage. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Thu Jan 31 03:01:09 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Jan 31 03:01:24 2008 Subject: AW: [Histonet] In Situ Hybridization Blue Background problem In-Reply-To: <8556a07e0801300813o67fe5e3cnbb6e598124bebf00@mail.gmail.com> Message-ID: <003601c863e7$d260bed0$eeeea8c0@dielangs.at> Just a thought. Do you dry your slides in an oven before staining? We have noticed some strange effect with charged slides, when we did FISH on cytopreps using charged slides. There was an enormous flourescent background, when we let them just airdry. This background was diminished, when we put them into the 60?C oven for half an hour. So I think, that the adhesive can be "destroyed" with heat, and therefore decreases the chance for catching detection-reagens. It can also be a matter of insufficient washing with the vortex-stations. When we had problems with patchy staining, the solutions was finally the exchange of the complete slide-tableau to one with more vortex-stations. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Brandon Dornier Gesendet: Mittwoch, 30. J?nner 2008 17:13 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] In Situ Hybridization Blue Background problem I have an In Situ Hybridization question for you guys and gals. I'm currently performing In Situ HPV and EBV tests using an automated machine called the XT by Ventana. The tissue is parrifin embedded, cut at 4 microns and place on charged slides. NBT/ BCIP is used as our chromogen. I am beginning to notice some background staining that varies in intensity. When it occurs on our QC slide, it can be strong enough to fail a run. The background resembles a "blue haze" that can cover the tissue making interpretation difficult. At other times the blue haze surrounds the tissue rather than covering it which makes me believe that there could be some sort of hydrophobic or other chemical rxn taking place to shield the tissue. I'm not certain if these two problems are related, but both occur completely random and always vary in intensity. If I can find the culprit of this problem it would lead to cleaner slides and more conclusive resulting. If anybody can help it would be much appreciated. Thanks P.S. Ventana has been notified of the problem and engineers have been sent multiple times to try to figure it out. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kappeler <@t> patho.unibe.ch Thu Jan 31 06:53:29 2008 From: kappeler <@t> patho.unibe.ch (Andi Kappeler) Date: Thu Jan 31 06:53:58 2008 Subject: AW: [Histonet] Antibody query In-Reply-To: <3F328377AF4E23438E78234752652CE105D52618@uwhis-xchng7.uwhis.hosp.wisc.edu> References: <3F328377AF4E23438E78234752652CE105D52618@uwhis-xchng7.uwhis.hosp.wisc.edu> Message-ID: <002d01c86408$473d6120$17955c82@pi23> Hi Sally MLH-1, clone G168-15, BD Pharmingen, CatNo 550838; HIER-Citrate pH 6.0 MSH-2, clone FE11, Calbiochem (used to be Oncogene Research), CatNo NA27; HIER-Urea pH 9.5 MSH-6, clone 44, BD Transduction, CatNo 610919; HIER-Citrate pH 6.0 PMS-2, clone A16-4, BD Pharmingen, CatNo 556415; HIER-Citrate pH 6.0 No experience with Ventana (and therefore no information on dilutions). Hope this helps. Good luck with testing! Andi Kappeler Institute of Pathology, University of Bern, Switzerland -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Drew Sally A. Gesendet: Mittwoch, 30. Januar 2008 18:13 An: Histonet Betreff: [Histonet] Antibody query We are wondering what vendors people are using for the following antibodies; MLH-1, MSH-2, MSH-6 and PMS-2. Anyone running these on the Ventana immunostainers? Thank you for any and all input! Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Thu Jan 31 06:58:04 2008 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Thu Jan 31 06:58:44 2008 Subject: [Histonet] New book for my library??? In-Reply-To: <715969.77537.qm@web90308.mail.mud.yahoo.com> Message-ID: <979FF5962E234F45B06CF0DB7C1AABB214FB0BEB@chi2k3ms01.columbuschildrens.net> Without doubt, this is the most comprehensive textbook on Histological technology in print. Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 Ronald.Houston@NationwideChildrens.org Columbus Children's Hospital is now Nationwide Children's Hospital www.NationwideChildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shelly Coker Sent: Wednesday, January 30, 2008 8:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New book for my library??? Hello all, I am considering adding a new text to my library: Theory and Practice of Histological Techniques by John D. Bancroft and Marilyn Gamble. Does anyone own this book? And if so, what do you think of it? The biggest reason I am hesitating is that the book is close to $200 and if it really isn't useful, I would rather not spend the money! Thanks, Shelly --------------------------------- Never miss a thing. Make Yahoo your homepage. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From relia1 <@t> earthlink.net Thu Jan 31 07:07:43 2008 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Jan 31 07:08:07 2008 Subject: [Histonet] RELIA Job Alert 01/31/08 New Opportunities for you or someone you know! Message-ID: Hello Histonetters! HOT HOT HOT!!! New opportunities for you or someone you know. I have several new positions that I want to tell you about. These are all full time day shift positions with companies who are responsive to your needs and appreciate your skills and talents. Take a look at the positions listed below and if you or someone you know might be interested in any of them please let me know. My clients offer excellent salaries, benefits and relocation assistance. Here are my newest positions: Research Histology Supervisor ? Seattle Immunohistochemistry Supervisor ? Dallas Research Histology Tech ? Seattle Histo Tech ? Corpus Christi Histo Tech ? South Carolina Histo Tech ? New Hampshire IHC Tech ? Waco Histo Tech ? Austin I also have positions in Los Angeles, Florida, Montana and Ohio. If you or someone you know is interested in any of these opportunities please let me know. Also please feel free to forward this e-mail to your friends and colleagues as well I can be reached toll free at 866-60-RELIA (866-607-3542) or relia1@earthlink.net Thanks-Pam Thank You! Pam M. Barker President Relia 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll Free: (866)607-3542 e-mail: relia1@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia From PMcArdle <@t> ebsciences.com Thu Jan 31 07:20:36 2008 From: PMcArdle <@t> ebsciences.com (Phil McArdle) Date: Thu Jan 31 07:20:50 2008 Subject: [Histonet] Microwave Container Use Message-ID: <47A1CB24.2000206@ebsciences.com> Hello all - This is a question from a vendor; I'm not trying to sell anything, so please wait before you flame! :-) We're looking for input regarding what container(s) (brand, type, size, etc.) people are using in microwaves in a laboratory context, good/bad experiences, leaks/failures with particular reagents over time, etc. I know how hectic things can be in the lab, so any information would be appreciated. This has more to do with "advancing the art" and recommendations/contraindications, than sales or product development. Responses can be public or private, as far as I'm concerned. Best regards, Phil McArdle -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com I skate to where the puck is going to be, not to where it's been. - Wayne Gretsky You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway. So please do the right thing and make this e-mail go away. Thank you. From fudo <@t> ufl.edu Thu Jan 31 07:37:40 2008 From: fudo <@t> ufl.edu (FU,DONGTAO) Date: Thu Jan 31 07:37:55 2008 Subject: [Histonet] fluorescent staining on xenograft tissues Message-ID: <1910323983.115741201786660025.JavaMail.osg@osgjas03.cns.ufl.edu> Hi,all I will start fluorescent staining on xenograft tissue(human cells inject into Scid mice) using Ms monoclonal antibody against human origin(like CD antibodies). So there raise a problem how to reduce background on xenografts when you use mouse monoclonal antibody? Does anyone have any experience or any protocol I can share? Thank you, Ann Dongtao Fu MD, Ph.D Lab Manager Dept. of Pathology Lab phone: 352-273-7752 Lab FAX: 352-273-7755 Lab address: D11-50 PO Box: 100275 1600 SW Archer Road University of Flodrida Gainesville, FL 32610 From fudo <@t> ufl.edu Thu Jan 31 07:37:48 2008 From: fudo <@t> ufl.edu (FU,DONGTAO) Date: Thu Jan 31 07:38:03 2008 Subject: [Histonet] fluorescent staining on xenograft tissues Message-ID: <1493285572.115751201786668740.JavaMail.osg@osgjas03.cns.ufl.edu> Hi,all I will start fluorescent staining on xenograft tissue(human cells inject into Scid mice) using Ms monoclonal antibody against human origin(like CD antibodies). So there raise a problem how to reduce background on xenografts when you use mouse monoclonal antibody? Does anyone have any experience or any protocol I can share? Thank you, Ann Dongtao Fu MD, Ph.D Lab Manager Dept. of Pathology Lab phone: 352-273-7752 Lab FAX: 352-273-7755 Lab address: D11-50 PO Box: 100275 1600 SW Archer Road University of Flodrida Gainesville, FL 32610 From ian.montgomery <@t> bio.gla.ac.uk Thu Jan 31 07:48:39 2008 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Thu Jan 31 07:48:53 2008 Subject: [Histonet] Histology text. Message-ID: <001701c8640f$fc386320$6424d182@IBLS.GLA.AC.UK> If you can find a copy, have a look at John Kiernan's book, Theory and Practice: Histological & Histochemical Methods. For me this is a definitive text. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, G12 8QQ. From karenadams <@t> comcast.net Thu Jan 31 07:58:08 2008 From: karenadams <@t> comcast.net (karenadams@comcast.net) Date: Thu Jan 31 07:58:21 2008 Subject: [Histonet] OSHA query... Message-ID: <013120081358.28926.47A1D3F000012A8C000070FE22070229339C030E0B0E020A9D0E05@comcast.net> ....I have not researched this topic yet....just throwing this out....we have a situation where our formalin PEL are within exposure limitations for 8hr and STEL in our grossing area...however, we have an employee who suffers from severe asthma. About 4 times/yr he develops a pneumonia and misses work for 6-8 days or so. My question is should we be providing him additional protection even though we are within established PEL's? -- Karen Adams Supervisor Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 From asmith <@t> mail.barry.edu Thu Jan 31 08:03:50 2008 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Thu Jan 31 08:05:11 2008 Subject: [Histonet] Histology text. In-Reply-To: <001701c8640f$fc386320$6424d182@IBLS.GLA.AC.UK> Message-ID: Kiernan's book is excellent, but it is out of print. Used copies are going for about the same price as a new copy of Bancroft and Gamble's book. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian Montgomery Sent: Thursday, January 31, 2008 8:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology text. If you can find a copy, have a look at John Kiernan's book, Theory and Practice: Histological & Histochemical Methods. For me this is a definitive text. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, G12 8QQ. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Jan 31 08:18:06 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 31 08:18:25 2008 Subject: [Histonet] ISH double staining In-Reply-To: <958077.15969.qm@web55812.mail.re3.yahoo.com> Message-ID: <645500.74421.qm@web61214.mail.yahoo.com> Yes, it is. You will have to use two different colored chromogens to be able to distinguish each Ag-Ab reaction. Ren? J. San Tin wrote: Hi all, Any one out there with experience of ISH double staining with two different probes? Do you think it is possible? San ____________________________________________________________________________________ Never miss a thing. Make Yahoo your home page. http://www.yahoo.com/r/hs _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From rjbuesa <@t> yahoo.com Thu Jan 31 08:19:56 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 31 08:20:07 2008 Subject: [Histonet] Microwave Container Use In-Reply-To: <47A1CB24.2000206@ebsciences.com> Message-ID: <31521.93048.qm@web61216.mail.yahoo.com> I always used ANY plastic sturdy containers I could fing at stores like Kmart, Target of Walmart. Ren? J. Phil McArdle wrote: Hello all - This is a question from a vendor; I'm not trying to sell anything, so please wait before you flame! :-) We're looking for input regarding what container(s) (brand, type, size, etc.) people are using in microwaves in a laboratory context, good/bad experiences, leaks/failures with particular reagents over time, etc. I know how hectic things can be in the lab, so any information would be appreciated. This has more to do with "advancing the art" and recommendations/contraindications, than sales or product development. Responses can be public or private, as far as I'm concerned. Best regards, Phil McArdle -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com I skate to where the puck is going to be, not to where it's been. - Wayne Gretsky You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway. So please do the right thing and make this e-mail go away. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From rjbuesa <@t> yahoo.com Thu Jan 31 08:27:45 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 31 08:28:00 2008 Subject: [Histonet] OSHA query... In-Reply-To: <013120081358.28926.47A1D3F000012A8C000070FE22070229339C030E0B0E020A9D0E05@comcast.net> Message-ID: <40165.16649.qm@web61212.mail.yahoo.com> IF his asthma is caused by YOUR formalin levels, you most certainly have to provide him additional protection and I would even change him from duties in that area. You have to remember that formalin limits are general guidelines but that there can be individuals who have lower thresholds and your employee could one of those. You should not subject him to levels he cannot tolerate, for whatever reason it may be. Ren? J. karenadams@comcast.net wrote: ....I have not researched this topic yet....just throwing this out....we have a situation where our formalin PEL are within exposure limitations for 8hr and STEL in our grossing area...however, we have an employee who suffers from severe asthma. About 4 times/yr he develops a pneumonia and misses work for 6-8 days or so. My question is should we be providing him additional protection even though we are within established PEL's? -- Karen Adams Supervisor Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From jmahoney <@t> alegent.org Thu Jan 31 08:36:35 2008 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Thu Jan 31 08:39:35 2008 Subject: [Histonet] Article in Advance In-Reply-To: <645500.74421.qm@web61214.mail.yahoo.com> References: <958077.15969.qm@web55812.mail.re3.yahoo.com> <645500.74421.qm@web61214.mail.yahoo.com> Message-ID: <47A188930200003C0002997B@gwia.alegent.org> Hello Rene, Great article in ADVANCE! The info provided is enlightening and helpful. It is nice to see more press on the wonderful field of Histotechnology. Jan Mahoney ALegent Helath Omaha, NE From Jason.Burrill <@t> crl.com Thu Jan 31 09:01:57 2008 From: Jason.Burrill <@t> crl.com (Burrill, Jason) Date: Thu Jan 31 09:02:22 2008 Subject: [Histonet] OSHA query Message-ID: <1AD4E907E9B6F648AEF1B3A20A9B0E1EAE1125@shr-exch2.na01.crl.com> Karen, Although you may be within the acceptable limits for OSHA's established PEL and STEL for formaldehyde but there is also an OSHA "Action Level" of .5 ppm. Do you know whether or not you are below this action level? Either way, Ren? brings up a good point that these are guidelines and not all individuals are created equal. OSHA bases these "acceptable" permissible exposure limits on an exposed worker being a "healthy male adult weighing 150 lbs". Based on this employee's medical predisposition of asthma you should make every effort to remove them from a situation where they would exposed to any hazardous substance that would potentially make them ill because of their medical problem. Also since this person has a medical illness that affects their breathing additional personal protective equipment (e.g. respirator) will most likely not work as the medical professional evaluating their medical questionnaire for respirator clearance would clear them based on their medical history. As a standard practice you should always use administrative (e.g. procedural modifications) and engineering controls (e.g. ventilated workspaces) to minimize any employee occupational exposure to a hazardous material before implementing personal protective equipment. Finally I would review the situation with your chemical hygiene officer and/or EHS department to help come up with a solution and avoid any non-compliance or possible legal ramifications of not providing a safe workplace. I hope this helps. Jason Jason Burrill Sr. Manager, Histology and Laboratory Safety Charles River Laboratories 251 Ballardvale Street Wilmington, MA 01887 Ph: 781-222-6152 Fax: 978-988-8793 jason.burrill@crl.com **Please note new direct dial telephone number** From jmjohnson34 <@t> hotmail.com Thu Jan 31 10:07:28 2008 From: jmjohnson34 <@t> hotmail.com (Jennifer Johnson) Date: Thu Jan 31 10:07:42 2008 Subject: [Histonet] Surpath prep-stain Slide Processor Message-ID: We do about 1,000 liquid paps/ year and need to find a used or refurbished machine to do the BP surepath pap smears. Does anyone have any suggestions or equipment for sale. Thanks, Jennifer P.S. We are not opposed to finding a reference lab if the price is right. _________________________________________________________________ Connect and share in new ways with Windows Live. http://www.windowslive.com/share.html?ocid=TXT_TAGHM_Wave2_sharelife_012008 From Dorothy.L.Webb <@t> HealthPartners.Com Thu Jan 31 10:20:47 2008 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Thu Jan 31 10:21:05 2008 Subject: [Histonet] Microwave bowls Message-ID: <0E394B648E5284478A6CCB78E5AFDA270563544A@hpes1.HealthPartners.int> We purchased pyrex bowls, the same as was originally sent with our microwave processor. It has been shown that several of the plastic containers can break down after repeated microwave use and we use our processor for multiple daily runs!! They were not expensive at Walmart, etc.! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From integrated.histo <@t> gmail.com Thu Jan 31 10:56:13 2008 From: integrated.histo <@t> gmail.com (Cindy DuBois) Date: Thu Jan 31 10:56:25 2008 Subject: [Histonet] Formalin Exposure Message-ID: <5d9104a30801310856y186f1b5ax6c51dbf6e3adc2e0@mail.gmail.com> I too have an employee with sometimes severe asthma. I told her to tell me when she is having a bad day / week, to let me know and I move her from the grossing area. Yes, I do get some complaints from other employees. But I explained to them I would rather have the one person healthy and able to work than have her off for several days due to her asthma. And if she had a severe attack due to formalin, I believe that would become a workman's comp issue. Cindy DuBois From mmmmt <@t> hotmail.com Thu Jan 31 11:00:04 2008 From: mmmmt <@t> hotmail.com (M T) Date: Thu Jan 31 11:00:19 2008 Subject: [Histonet] zn-formalin Message-ID: Hello Colleagues, Can any body tell me how can I prepare buffered ZN-formalin? Have a nice day Mike _________________________________________________________________ Windows Live Fotogalerie: So einfach organisieren Sie Ihre Fotos! http://get.live.com/photogallery/overview From peter.craven <@t> haht.scot.nhs.uk Thu Jan 31 11:27:45 2008 From: peter.craven <@t> haht.scot.nhs.uk (PETER CRAVEN) Date: Thu Jan 31 11:28:17 2008 Subject: [Histonet] unsubscribe References: <200801311428.BKC13820@rlmh03.swi.contact.secure-ops.net> Message-ID: <70B1BBF61EF50C488725E84760EC7BFF020CA2E3@NHSHRMDC02.NHSH.SCOT.NHS.UK> ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of histonet-request@lists.utsouthwestern.edu Sent: Thu 31/01/2008 14:28 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 50, Issue 42 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Please consider the environment before printing this e-mail ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager. This footnote also confirms that this email message has been swept by MIMEsweeper for the presence of computer viruses. www.mimesweeper.com ********************************************************************** Please consider the environment before printing this e-mail From LuckG <@t> empirehealth.org Thu Jan 31 11:53:54 2008 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Thu Jan 31 11:54:07 2008 Subject: [Histonet] P16 Ab. Message-ID: <6BB8BC4519AAB844B174FC739A679BBCCEFFF5@IRMEXCH01.irm.inhs.org> Hello All, I know this was knocked around a few weeks ago but I need to order what is now the only available option (i.e. in kit form) P16 antibody. Could anybody supply me with the only apparent vendor and cat# (if possible) for this kit. Thanks, Greg Greg Luck, B.S., HT(ASCP) Anatomic Pathology Supervisor Deaconess Med Cntr 800 W. 5th Ave Spokane, WA 99204 Offc 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmc.org From gayle.callis <@t> bresnan.net Thu Jan 31 11:56:31 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Thu Jan 31 11:56:46 2008 Subject: [Histonet] zn-formalin References: Message-ID: <000a01c86432$9c597570$6501a8c0@DHXTS541> We preferred to buy a concentrated Zinc formalin from Anatech. The reason is that is never precipitated, was buffered, with excellent fixation. Other companies will have it also. There are recipes in Histo Logic on the Sakura Finetek website. Some techs have complained about in house preparations precipitating, but the Anatech never did this for us. Good luck Gayle M. Callis HT/HTL/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "M T" To: Sent: Thursday, January 31, 2008 10:00 AM Subject: [Histonet] zn-formalin Hello Colleagues, Can any body tell me how can I prepare buffered ZN-formalin? Have a nice day Mike _________________________________________________________________ Windows Live Fotogalerie: So einfach organisieren Sie Ihre Fotos! http://get.live.com/photogallery/overview _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Thu Jan 31 12:03:14 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Thu Jan 31 12:03:26 2008 Subject: [Histonet] fluorescent staining on xenograft tissues References: <1910323983.115741201786660025.JavaMail.osg@osgjas03.cns.ufl.edu> Message-ID: <003801c86433$8c8cda50$6501a8c0@DHXTS541> You may be able to use the DAKO ARK kit for this. There is an excellent publication in Journal of Histochemistry and Cytochemistry by Dr. Chris van der Loos, on the use od ARK with monoclonals on human tissues. Hopefully, Chris is looking in and can comment on this. Gayle M. Callis HT/HTL/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "FU,DONGTAO" To: Sent: Thursday, January 31, 2008 6:37 AM Subject: [Histonet] fluorescent staining on xenograft tissues > Hi,all > > I will start fluorescent staining on xenograft tissue(human cells inject > into Scid mice) using Ms monoclonal antibody against human origin(like CD > antibodies). So there raise a problem how to reduce background on > xenografts when you use mouse monoclonal antibody? Does anyone have any > experience or any protocol I can share? > > Thank you, > > Ann Dongtao Fu MD, Ph.D > Lab Manager > Dept. of Pathology > Lab phone: 352-273-7752 > Lab FAX: 352-273-7755 > Lab address: D11-50 > PO Box: 100275 > 1600 SW Archer Road > University of Flodrida > Gainesville, FL 32610 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Thu Jan 31 12:07:06 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Jan 31 12:07:21 2008 Subject: [Histonet] P16 Ab. In-Reply-To: <6BB8BC4519AAB844B174FC739A679BBCCEFFF5@IRMEXCH01.irm.inhs.org> References: <6BB8BC4519AAB844B174FC739A679BBCCEFFF5@IRMEXCH01.irm.inhs.org> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82AD3@EMAIL.archildrens.org> MTM Laboratories, Inc 134 Flanders Road, Suite 325 Westborough, MA 01581 1.866.686.5227 Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Luck, Greg D. Sent: Thursday, January 31, 2008 11:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] P16 Ab. Hello All, I know this was knocked around a few weeks ago but I need to order what is now the only available option (i.e. in kit form) P16 antibody. Could anybody supply me with the only apparent vendor and cat# (if possible) for this kit. Thanks, Greg Greg Luck, B.S., HT(ASCP) Anatomic Pathology Supervisor Deaconess Med Cntr 800 W. 5th Ave Spokane, WA 99204 Offc 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Thu Jan 31 12:20:46 2008 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Thu Jan 31 12:21:30 2008 Subject: [Histonet] CD103 Message-ID: <979FF5962E234F45B06CF0DB7C1AABB214FB134E@chi2k3ms01.columbuschildrens.net> Anyone aware of an antibody for CD103 that works on paraffin sections? Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 Ronald.Houston@NationwideChildrens.org Columbus Children's Hospital is now Nationwide Children's Hospital www.NationwideChildrens.org ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From Dorothy.L.Webb <@t> HealthPartners.Com Thu Jan 31 12:27:08 2008 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Thu Jan 31 12:27:21 2008 Subject: [Histonet] Cap today Message-ID: <0E394B648E5284478A6CCB78E5AFDA270563544D@hpes1.HealthPartners.int> Did everyone read the 2 articles in the January 2008 issue of "CAP TODAY" concerning IHC analysis and the standards of the future and the article on estrogen receptor testing? They are excellent articles and the one on estrogen testing is excellent to bring the point home to those who do the grossing in of optimal size of tissue submitted!! I made copies and highlighted for my PA's!! Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From CIngles <@t> uwhealth.org Thu Jan 31 12:43:08 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Thu Jan 31 12:44:56 2008 Subject: [Histonet] Article in Advance References: <958077.15969.qm@web55812.mail.re3.yahoo.com><645500.74421.qm@web61214.mail.yahoo.com> <47A188930200003C0002997B@gwia.alegent.org> Message-ID: <08A0A863637F1349BBFD83A96B27A50A1200D7@uwhis-xchng3.uwhis.hosp.wisc.edu> I second that! Even magazines like Advance seem to me to short-shift the histotechs on relevant articles. Maybe I need to start writing some... Thanks for the info Rene. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Janice Mahoney Sent: Thu 1/31/2008 8:36 AM To: histonet@lists.utsouthwestern.edu; Rene J Buesa; San Tin Subject: [Histonet] Article in Advance Hello Rene, Great article in ADVANCE! The info provided is enlightening and helpful. It is nice to see more press on the wonderful field of Histotechnology. Jan Mahoney ALegent Helath Omaha, NE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cad <@t> Stowers-Institute.org Thu Jan 31 14:24:40 2008 From: cad <@t> Stowers-Institute.org (Dickey, Coral) Date: Thu Jan 31 14:25:11 2008 Subject: [Histonet] Xray contrast Message-ID: Hello in Histoland, I am trying to track the decalcification of some 42 day old mouse humeri and femurs by xray. They have been in 10% EDTA for 36hrs and I just took a film on KODAK BioMax MS film on a Faxitron xray machine. The bones were exposed for 30 sec at 30kvp. I would like to increase the contrast on the picture. Does anyone know how to do this. Thanks in advance. Coral Dickey Histology Specialist I Stowers Institute for Medical Research 1000 E. 50th Street Kansas City, Missouri 64110 Phone: 816-926-4305 e-mail: cad@stowers-institute.org From kellerc2 <@t> uthscsa.edu Thu Jan 31 14:45:32 2008 From: kellerc2 <@t> uthscsa.edu (Keller, Charles) Date: Thu Jan 31 14:46:54 2008 Subject: [Histonet] Xray contrast In-Reply-To: References: Message-ID: Dear Coral, you may be decalcifying the specimen so that it will be hard to take a good picture. Changing the buffer to PBS will improve the contrast above background. If it isn't an issue with sectioning, you can transition it to 100% ethanol for much better contrast. Averaging several digital exposures will also increase signal:noise. I can also do a nice overnight scan with our microCT (http://ccri.uthscsa.edu/MicroCT.html) at no charge if you wish. Sincerely, Charles Charles Keller, MD Assistant Professor, Department of Cellular & Structural Biology Adjunct Assistant Professor, Department of Pediatrics Director, Small Animal Imaging Facility Greehey Children's Cancer Research Institute The University of Texas Health Science Center 8403 Floyd Curl Drive, Mail Code 7784 San Antonio, TX 78229-3900 210-562-9062 [office] 210-562-9014 [fax] http://gccri.uthscsa.edu or www.sarcomalab.org kellerc2@uthscsa.edu Postdoctoral Opportunities and Undergraduate & Summer Internships: http://ccri.uthscsa.edu/keller CCRI and UTHSCSA wish to promote open communication while protecting confidential and/or privileged information. If you have received this message in error, please inform the sender and delete all copies. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dickey, Coral Sent: Thursday, January 31, 2008 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xray contrast Hello in Histoland, I am trying to track the decalcification of some 42 day old mouse humeri and femurs by xray. They have been in 10% EDTA for 36hrs and I just took a film on KODAK BioMax MS film on a Faxitron xray machine. The bones were exposed for 30 sec at 30kvp. I would like to increase the contrast on the picture. Does anyone know how to do this. Thanks in advance. Coral Dickey Histology Specialist I Stowers Institute for Medical Research 1000 E. 50th Street Kansas City, Missouri 64110 Phone: 816-926-4305 e-mail: cad@stowers-institute.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Thu Jan 31 15:25:35 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Thu Jan 31 15:25:55 2008 Subject: [Histonet] Xray contrast References: Message-ID: <000701c8644f$d113aa20$6501a8c0@DHXTS541> Coral, You need to calibrate your FAXITRON with a normal mouse femur and tibia for the film you are using. We used Kodak XOMAT TL, self enclosed film which may not be available now. Linda Jenkins liked Kodak MIN R 2000 mammography film. It is a good idea to have a dried bone (even from a 42 day old mouse) sitting around for posterity sake and further calibration when needed. When we did xray endpoint checks, we only exposed these very tiny bones for 10 seconds or less, with bone on lower shelf. Sometimes no more than an on/off working of the exposure. Mouse bones, and especially young mice may not have as much calcification or thin areas of calcification, so that your "burn" in the bone too much i.e. overexpose the bone. We did our radiography manually. With 30 sec at 30 kvp, you are probably over-exposing the bone, and not able to see any tiny calcifications remaining in the bone. You can also use less kvp for these tiny bones and inversely, use much higher kvp for whole sheep knees (with longer times too). Be sure to examine your developed radiographs in a darkened room, with a magnifying glass to look for any tiny calcifications in the bone near endpoint. Also, if you have a mini CT scanner available, that is an excellent device for doing endpoint checks. Gayle M. Callis HT/HTL/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Dickey, Coral" To: Sent: Thursday, January 31, 2008 1:24 PM Subject: [Histonet] Xray contrast Hello in Histoland, I am trying to track the decalcification of some 42 day old mouse humeri and femurs by xray. They have been in 10% EDTA for 36hrs and I just took a film on KODAK BioMax MS film on a Faxitron xray machine. The bones were exposed for 30 sec at 30kvp. I would like to increase the contrast on the picture. Does anyone know how to do this. Thanks in advance. Coral Dickey Histology Specialist I Stowers Institute for Medical Research 1000 E. 50th Street Kansas City, Missouri 64110 Phone: 816-926-4305 e-mail: cad@stowers-institute.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Thu Jan 31 15:46:20 2008 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Jan 31 15:46:23 2008 Subject: [Histonet] Formalin Exposure In-Reply-To: <5d9104a30801310856y186f1b5ax6c51dbf6e3adc2e0@mail.gmail.com> Message-ID: Just out of curiosity, what clearing agent are you using in the lab? Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Cindy DuBois" Sent by: histonet-bounces@lists.utsouthwestern.edu 01/31/2008 08:56 AM To karenadams@comcast.net, Histonet cc Subject [Histonet] Formalin Exposure I too have an employee with sometimes severe asthma. I told her to tell me when she is having a bad day / week, to let me know and I move her from the grossing area. Yes, I do get some complaints from other employees. But I explained to them I would rather have the one person healthy and able to work than have her off for several days due to her asthma. And if she had a severe attack due to formalin, I believe that would become a workman's comp issue. Cindy DuBois _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Thu Jan 31 15:46:38 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Thu Jan 31 15:46:49 2008 Subject: [Histonet] Round lens paper Message-ID: <57BE698966D5C54EAE8612E8941D768302589569@EXCHANGE3.huntingtonhospital.com> Does anyone know where I can order round lens paper (not filter paper)? We place these in the little disposable funnels and filter our GI specimens through it. The specimen is then wrapped in this paper and placed in a cassette. Our current vendor is unreliable. Laurie Colbert From gayle.callis <@t> bresnan.net Thu Jan 31 15:57:21 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Thu Jan 31 15:57:35 2008 Subject: [Histonet] Xray contrast References: Message-ID: <001301c86454$4142dab0$6501a8c0@DHXTS541> Dear Charles, Where were you and your micro CT scanner when I was doing all this stuff!!! We decalcified most murine bones with formic acid, rinsed with water before radiographic (FAXITRON) checks. We still had to cut back on kvp and time with our non digital, old but beloved FAXITRON. The XOMAT film was high contrast if I remember correctly. EDTA + 100% alcohol will also form preciptates in the bone that you may not want to have for any future sectioning either. Also, Coral did not indicate that her FAXITRON was a digital machine? Gayle M. Callis HT/HTL/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Keller, Charles" To: "Dickey, Coral" ; Cc: "Prajapati, Suresh" Sent: Thursday, January 31, 2008 1:45 PM Subject: RE: [Histonet] Xray contrast Dear Coral, you may be decalcifying the specimen so that it will be hard to take a good picture. Changing the buffer to PBS will improve the contrast above background. If it isn't an issue with sectioning, you can transition it to 100% ethanol for much better contrast. Averaging several digital exposures will also increase signal:noise. I can also do a nice overnight scan with our microCT (http://ccri.uthscsa.edu/MicroCT.html) at no charge if you wish. Sincerely, Charles Charles Keller, MD Assistant Professor, Department of Cellular & Structural Biology Adjunct Assistant Professor, Department of Pediatrics Director, Small Animal Imaging Facility Greehey Children's Cancer Research Institute The University of Texas Health Science Center 8403 Floyd Curl Drive, Mail Code 7784 San Antonio, TX 78229-3900 210-562-9062 [office] 210-562-9014 [fax] http://gccri.uthscsa.edu or www.sarcomalab.org kellerc2@uthscsa.edu Postdoctoral Opportunities and Undergraduate & Summer Internships: http://ccri.uthscsa.edu/keller CCRI and UTHSCSA wish to promote open communication while protecting confidential and/or privileged information. If you have received this message in error, please inform the sender and delete all copies. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dickey, Coral Sent: Thursday, January 31, 2008 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xray contrast Hello in Histoland, I am trying to track the decalcification of some 42 day old mouse humeri and femurs by xray. They have been in 10% EDTA for 36hrs and I just took a film on KODAK BioMax MS film on a Faxitron xray machine. The bones were exposed for 30 sec at 30kvp. I would like to increase the contrast on the picture. Does anyone know how to do this. Thanks in advance. Coral Dickey Histology Specialist I Stowers Institute for Medical Research 1000 E. 50th Street Kansas City, Missouri 64110 Phone: 816-926-4305 e-mail: cad@stowers-institute.org From mpence <@t> grhs.net Thu Jan 31 16:36:23 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Jan 31 16:36:35 2008 Subject: [Histonet] Round lens paper In-Reply-To: <57BE698966D5C54EAE8612E8941D768302589569@EXCHANGE3.huntingtonhospital.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C8A1@IS-E2K3.grhs.net> Try tea bags: http://www.sunburstbottle.com/bags/tea-muslin Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, January 31, 2008 3:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Round lens paper Does anyone know where I can order round lens paper (not filter paper)? We place these in the little disposable funnels and filter our GI specimens through it. The specimen is then wrapped in this paper and placed in a cassette. Our current vendor is unreliable. Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rgarhart <@t> system1.net Thu Jan 31 16:41:16 2008 From: rgarhart <@t> system1.net (Robert Garhart) Date: Thu Jan 31 16:40:57 2008 Subject: [Histonet] Histology Job Opportunities In-Reply-To: <000201c77d29$5ef145f0$800aa8c0@domain.local> References: <000201c77d29$5ef145f0$800aa8c0@domain.local> Message-ID: Histonetters: There are some great opportunities for you waiting out there regardless of your experience level. We are working positions at the bench, supervisor and even AP manager level. Definite Positions: 1. Bench histotech Position in South Georgia. With a private and growing group. Prefer at least a few years of experience. 2. AP Manager in Oh. Looking for a HT supervisor or manager who is looking for a great career move. Must be able to manage relationships between 3 entities being the lab, the pathologists and the hospital. Great growth potential from this position as well. Potential Opportunities for interested parties: 1. Histology Supervisor in the greater Atlanta area. Must have supervisory or management experience. 2. Bench Histotech position potential in the northeast and southwest with rapidly growing independent lab. Please call Robert with any interest or pass along to those who might be interested. Robert Garhart Executive Recruiter System 1 Search 678-342-9029 Office rgarhart@system1.net Website: www.system1.net Please Join my Network on Linked In http://www.linkedin.com/in/robertgarhart From d.non <@t> verizon.net Thu Jan 31 17:01:25 2008 From: d.non <@t> verizon.net (DNon) Date: Thu Jan 31 17:04:07 2008 Subject: [Histonet] Re:Mucin containing cells too blue References: <0JV300B3KZH4QB55@vms169131.mailsrvcs.net> Message-ID: <007401c8645d$352dcfb0$1f0aa8c0@ocmedlab.loc> I had this very same issue with Surgipath Hematoxylin a few months ago. Jumped through hoops to try to fix the problem. Found a very effective and fast solution after getting excellent responses on Histonet: I changed brands of hematoxylin. Kept Surgipath reagents for the rest of our H&E staining, but switched to a rebranded Hematoxylin from a local source ( Belair Instruments in New Jersey...dont know who the actual manufacturer of the Hematoxylin is, but it works great). It seems more than a few other people have had the exact same issue with Surgipath hematoxlyin. Alot of people had switched to various different brands and fixed the problem. Its a shame as we used the Surgipath brand hematoxylin for 12 years without issue until this happened. D.Non Ocean County Medical Lab Brick, New Jersey > Date: Tue, 22 Jan 2008 16:24:05 -0400 > From: "Greg Dobbin" > Subject: [Histonet] Mucin containing cells too blue > To: > Message-ID: > Content-Type: text/plain; charset=US-ASCII > > Hi Folks, > I have been using the Surgipath Selectech reagents (H&E) for almost 3 > months now and I have not had a single complaint about staining...until > now. Suddenly the mucin containing cells are too blue. My first > thought was to make fresh Define (the Surgipath differentiator). This > did not fix the problem. Next I replaced the hematoxylin with fresh > (not supposed to have to change before 2500 slides, but changed at 1500 > slides to see if that would correct the problem). It has not. > > I was sure that fresh hematoxylin (and thererfore correct pH) would > rectify the problem. What are the other usual suspects here? I should > also mention our H&E's are done on the Leica Autostainer. > I look forward to receiving your collective insight! > Greg > > > > Greg Dobbin, R.T. > Chief Technologist, Histology Lab > Dept. of Laboratory Medicine, > Queen Elizabeth Hospital, > P.O. Box 6600 > Charlottetown, PE C1A 8T5 > Phone: (902) 894-2337 > Fax: (902) 894-2385 > From cad <@t> Stowers-Institute.org Thu Jan 31 17:06:05 2008 From: cad <@t> Stowers-Institute.org (Dickey, Coral) Date: Thu Jan 31 17:06:35 2008 Subject: [Histonet] Xray contrast Message-ID: Thanks for all your quick replies! Coral Dickey Histology Specialist I Stowers Institute for Medical Research 1000 E. 50th Street Kansas City, Missouri 64110 Phone: 816-926-4305 e-mail: cad@stowers-institute.org From sccrshlly <@t> yahoo.com Thu Jan 31 17:06:43 2008 From: sccrshlly <@t> yahoo.com (Shelly Coker) Date: Thu Jan 31 17:06:54 2008 Subject: [Histonet] Re: Slide Trays for Refrigeration of Paraffin Dipped Slides Message-ID: <163942.69175.qm@web90307.mail.mud.yahoo.com> Cardinal has metal slide trays that stack on top of one another. We use them in our oven, but they would also work in the refrigerator. If you are interested, let me know and I can get you the item number. Shelly --------------------------------- Never miss a thing. Make Yahoo your homepage. From cad <@t> Stowers-Institute.org Thu Jan 31 17:07:18 2008 From: cad <@t> Stowers-Institute.org (Dickey, Coral) Date: Thu Jan 31 17:07:45 2008 Subject: [Histonet] Xray contrast In-Reply-To: <001301c86454$4142dab0$6501a8c0@DHXTS541> Message-ID: The Faxitron is not digital. -----Original Message----- From: Gayle Callis [mailto:gayle.callis@bresnan.net] Sent: Thursday, January 31, 2008 3:57 PM To: Keller, Charles; Dickey, Coral; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Xray contrast Dear Charles, Where were you and your micro CT scanner when I was doing all this stuff!!! We decalcified most murine bones with formic acid, rinsed with water before radiographic (FAXITRON) checks. We still had to cut back on kvp and time with our non digital, old but beloved FAXITRON. The XOMAT film was high contrast if I remember correctly. EDTA + 100% alcohol will also form preciptates in the bone that you may not want to have for any future sectioning either. Also, Coral did not indicate that her FAXITRON was a digital machine? Gayle M. Callis HT/HTL/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Keller, Charles" To: "Dickey, Coral" ; Cc: "Prajapati, Suresh" Sent: Thursday, January 31, 2008 1:45 PM Subject: RE: [Histonet] Xray contrast Dear Coral, you may be decalcifying the specimen so that it will be hard to take a good picture. Changing the buffer to PBS will improve the contrast above background. If it isn't an issue with sectioning, you can transition it to 100% ethanol for much better contrast. Averaging several digital exposures will also increase signal:noise. I can also do a nice overnight scan with our microCT (http://ccri.uthscsa.edu/MicroCT.html) at no charge if you wish. Sincerely, Charles Charles Keller, MD Assistant Professor, Department of Cellular & Structural Biology Adjunct Assistant Professor, Department of Pediatrics Director, Small Animal Imaging Facility Greehey Children's Cancer Research Institute The University of Texas Health Science Center 8403 Floyd Curl Drive, Mail Code 7784 San Antonio, TX 78229-3900 210-562-9062 [office] 210-562-9014 [fax] http://gccri.uthscsa.edu or www.sarcomalab.org kellerc2@uthscsa.edu Postdoctoral Opportunities and Undergraduate & Summer Internships: http://ccri.uthscsa.edu/keller CCRI and UTHSCSA wish to promote open communication while protecting confidential and/or privileged information. If you have received this message in error, please inform the sender and delete all copies. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dickey, Coral Sent: Thursday, January 31, 2008 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xray contrast Hello in Histoland, I am trying to track the decalcification of some 42 day old mouse humeri and femurs by xray. They have been in 10% EDTA for 36hrs and I just took a film on KODAK BioMax MS film on a Faxitron xray machine. The bones were exposed for 30 sec at 30kvp. I would like to increase the contrast on the picture. Does anyone know how to do this. Thanks in advance. Coral Dickey Histology Specialist I Stowers Institute for Medical Research 1000 E. 50th Street Kansas City, Missouri 64110 Phone: 816-926-4305 e-mail: cad@stowers-institute.org From CIngles <@t> uwhealth.org Thu Jan 31 18:02:31 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Thu Jan 31 18:02:44 2008 Subject: [Histonet] Re:Mucin containing cells too blue References: <0JV300B3KZH4QB55@vms169131.mailsrvcs.net> <007401c8645d$352dcfb0$1f0aa8c0@ocmedlab.loc> Message-ID: <08A0A863637F1349BBFD83A96B27A50A1200D9@uwhis-xchng3.uwhis.hosp.wisc.edu> Speaking of Surgipath Hematoxylin... Has anyone heard of their new Hematoxylin MX (Selectech line) specifically for frozens that is currently in "Beta" testing? (my co-worker has said the 'Beta testing' part is just to get you get the rep to show you the product then to buy other stuff. i.e. bait and switch) is supposed to be darker than regular heme. How reproducible is it? i.e. 'idiot proof'. Is there a strength continuity between batches. How well does it age?We have a linear stainer with fixed times for each well, but also want to encorporate it into hand staining frozens and maybe even cut down on paraffin staining times. Thanks guys Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of DNon Sent: Thu 1/31/2008 5:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re:Mucin containing cells too blue I had this very same issue with Surgipath Hematoxylin a few months ago. Jumped through hoops to try to fix the problem. Found a very effective and fast solution after getting excellent responses on Histonet: I changed brands of hematoxylin. Kept Surgipath reagents for the rest of our H&E staining, but switched to a rebranded Hematoxylin from a local source ( Belair Instruments in New Jersey...dont know who the actual manufacturer of the Hematoxylin is, but it works great). It seems more than a few other people have had the exact same issue with Surgipath hematoxlyin. Alot of people had switched to various different brands and fixed the problem. Its a shame as we used the Surgipath brand hematoxylin for 12 years without issue until this happened. D.Non Ocean County Medical Lab Brick, New Jersey > Date: Tue, 22 Jan 2008 16:24:05 -0400 > From: "Greg Dobbin" > Subject: [Histonet] Mucin containing cells too blue > To: > Message-ID: > Content-Type: text/plain; charset=US-ASCII > > Hi Folks, > I have been using the Surgipath Selectech reagents (H&E) for almost 3 > months now and I have not had a single complaint about staining...until > now. Suddenly the mucin containing cells are too blue. My first > thought was to make fresh Define (the Surgipath differentiator). This > did not fix the problem. Next I replaced the hematoxylin with fresh > (not supposed to have to change before 2500 slides, but changed at 1500 > slides to see if that would correct the problem). It has not. > > I was sure that fresh hematoxylin (and thererfore correct pH) would > rectify the problem. What are the other usual suspects here? I should > also mention our H&E's are done on the Leica Autostainer. > I look forward to receiving your collective insight! > Greg > > > > Greg Dobbin, R.T. > Chief Technologist, Histology Lab > Dept. of Laboratory Medicine, > Queen Elizabeth Hospital, > P.O. Box 6600 > Charlottetown, PE C1A 8T5 > Phone: (902) 894-2337 > Fax: (902) 894-2385 > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu Jan 31 18:35:25 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Jan 31 18:35:37 2008 Subject: [Histonet] CD10 Antibody for mouse tissues - paraffin Message-ID: Hello everyone Is anyone aware of a CD10 antibody that works in murine tissue samples, paraffin would be great but frozen will also work. I searched quite a bit and did see a few antibodies that would work in paraffin and also cross react with mouse, but I could not locate any publications or images, any help would be appreciated. I just hate to purchase an antibody just based upon the information in the specification sheet. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 From WWmn916 <@t> aol.com Thu Jan 31 19:46:52 2008 From: WWmn916 <@t> aol.com (WWmn916@aol.com) Date: Thu Jan 31 19:47:08 2008 Subject: [Histonet] Silica water testing Message-ID: Hello everyone, I have three questions about silica testing for Type II water: 1) NCCLS guidelines state max silica content at 0.1 mg/L and that silicates may interfere with certain assays. What assays specifically and briefly why? IPOX staining? 2) Can water from same source be tested as Type II for microbial content and tested as Type III for silica? 3) What do others do about silica levels that are above NCCLS guidelines? Thanks in advance Deb King, HT Sacramento, CA **************Start the year off right. 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