[Histonet] M'Fadyean Staining of Bacillus anthracis

[Owen, Michael P]

Dear List Members,

Do any of you still perform M'Fadyean (polychrome methylene blue)
staining to visualize the poly-D-glutamic capsule of Bacillus anthracis?
Did you encounter problems with the procedure? 

I am very interested in your experiences with the method including
limitations encountered.

You can contact me off the list to give responses. Thanks in advance.


Michael P. Owen, Regulatory Microbiologist
U.S. FDA Pacific Regional Lab Northwest
22201 23rd Drive SE  Bothell, WA 98021-4421
Phone: 425-483-4865     E-Mail: michael.owen <@t> fda.hhs.gov