AW: [Histonet] hb crystals

[Paul Bradbury]

I have never seen the PAS reaction give positive results with formalin 
pigment. Even if it did react, how would it be seen. The pigment is 
already dark brown-black, so colouring it red would have no visible 
effect. The same problem applies to any silver methods which might work 
... it's already almost black, so making it a "darker black" would not 
be much help. The only definitive way to identify it is to use its 
birefringent properties.

Paul
Kamloops, Canada


Gudrun Lang wrote:
> If this crystals are formalin-pigment they have reducing capacitiy.
> Therefore with silverimpregnation techniques like Masson this would render
> black spots. Also the PAS stain would give positivity.
>
> Gudrun Lang
>  
> Biomed. Analytikerin
> Histolabor
> Akh Linz
> Krankenhausstr. 9
> 4020 Linz
> +43(0)732/7806-6754
> -----Ursprüngliche Nachricht-----
> Von: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Jim
> Manavis
> Gesendet: Donnerstag, 28. Februar 2008 03:41
> An: Histonet
> Betreff: [Histonet] hb crystals
>
> Dear Sir or Madam:
>
>  
>
> I am a PhD student studying intracerebral haemorrhage in the rat and have
> found numerous crystals contained within the haemorrhage, which I presume
> are haemoglobin crystals, although it would be nice to demonstrate this.
> Pearl's stain doesn't work, presumably because the iron is ferrous and bound
> tightly, and Lillie's method with ferricyanide and HCL likewise doesn't
> work, presumably because the iron is bound. Our standard DAB technique for
> immunohistochemistry doesn't seem to react either. How might I best
> determine whether or not the crystals are haemoglobin crystals?
>
>  
>
> Yours Sincerely, 
>
>  
>
> Tim Kleinig
>
>  
>
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