[Histonet] FW: under processed tissue

[Cheri Miller]

Cheryl Miller HT (ASCP)

Histology Supervisor

Physicians Laboratory,P.C.

Omaha, Ne. 

402 738 5052

  _____  

From: Cheri Miller [mailto:cmiller <@t> physlab.com] 
Sent: Thursday, February 28, 2008 11:31 AM
To: histonet-bounces <@t> lists.utsouthwestern.edu
Subject: FW: under processed tissue

 

 

 

Cheryl Miller HT (ASCP)

Histology Supervisor

Physicians Laboratory,P.C.

Omaha, Ne. 

402 738 5052

  _____  

From: Cheri Miller [mailto:cmiller <@t> physlab.com] 
Sent: Thursday, February 28, 2008 11:01 AM
Cc: 'mfokol <@t> sakuraus.com'
Subject: under processed tissue

 

Hi, I hope someone out there can help me. I am having issues with
under-processed tissue. A few weeks ago we had power outages and our VIP
2000 battery backup didn't work. We have since fixed this problem and
everything on the VIP is working as before. My problem is I had to set up my
processing programs again.( the programs were set prior to my employment) I
suspect my times are not the same in each station  as before and are causing
the subtle and not so subtle changes I am seeing. Every thing is cutting
well except the more fatty tissues (breasts, colons, lipomas etc.) I have
listed my routine program below.  I forgot to mention that we put our fatty
tissues in lilies fix (formaldehyde, alcohol and acetic acid)

For 3-5 hours prior to processing. 

 


Station

             Fluid

Time


1

Formalin 10%

60 mins


2

Formalin  10%

60 mins


3

70% Reagent alcohol

45 mins


4

95% Reagent alcohol

50 mins


5

95% Reagent alcohol

50 mins


6

100% Reagent alcohol

50 mins


7

100% Reagent alcohol

50 mins


8

100 % Reagent alcohol

50 mins


9

Xylene

45 mins


10

Xylene

45 mins


11

Empty/ paraffin bath

0


12

Paraffin

30 mins


13

Paraffin

30 mins


14

Paraffin

45 mins


15

Clean xylene

 


16

Clean alcohol

 

 

 

Cheryl Miller HT (ASCP)

Histology Supervisor

Physicians Laboratory,P.C.

Omaha, Ne. 

402 738 5052

 



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