[Histonet] RED COUNTERSTAINS

Mark Tarango marktarango <@t> gmail.com
Tue Feb 26 05:16:25 CST 2008


I like using Richard-Allen's 7211 hematoxylin for counterstains like in the
iron stain.  I do a minute w/ hematoxylin, rinse, and then a minute in their
clarifier II.  If you use distilled water the hematoxylin stays red and
looks great.  If you have any lying around, it can't hurt to try.



On Mon, Feb 25, 2008 at 11:53 AM, Diana McCaig <dmccaig <@t> ckha.on.ca> wrote:

> In the past we have had great red counterstains when using Nuclear Fast
> Red and Neutral Red.
>
> Recently it does not matter how fresh,  concentration  or times of these
> two stain, they appear
> pale and washed out.
>
> Any ideas what could be causing this or how it could be remedied.
>
> diana
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