[Histonet] RE: Histonet Digest, Vol 51, Issue 31

[Ralf van den Berg]

-----Oorspronkelijk bericht-----
Van: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Namens histonet-request <@t> lists.utsouthwestern.edu
Verzonden: woensdag 20 februari 2008 19:35
Aan: histonet <@t> lists.utsouthwestern.edu
Onderwerp: Histonet Digest, Vol 51, Issue 31

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Today's Topics:

   1. RE: Specimen Rejection - QA (Lee & Peggy Wenk)
   2. Pregnancy (Martin, Erin)
   3. What is your experience with Histo-Clear II (Breckenridge, Sue)
   4. Formalin and Xylene Free Histology - Any Info about Glyo	Fixx
      (McKnight, Tanisha)
   5. RE: What is your experience with Histo-Clear II
      (Douglas D Deltour)
   6. RE: SPAM-LOW: [Histonet] Formalin and Xylene Free Histology -
      Any	Info about Glyo Fixx (Douglas D Deltour)
   7. Re: What is your experience with Histo-Clear II (Rene J Buesa)
   8. Dako Info (Nita Searcy)
   9. Re: Dako Info (Patti Loykasek)
  10. IHC Detection systems (Patti Loykasek)
  11. Re: Dako Info (Dana Settembre)
  12. Join dalia el-rouby on Yahoo! Messenger!
      (daliaelrouby <@t> hotmail.com)
  13. RE: Dako Info (Weems, Joyce)
  14. Adhesive tape & MMA (Lynne Cates)


----------------------------------------------------------------------

Message: 1
Date: Wed, 20 Feb 2008 06:44:40 -0500
From: "Lee & Peggy Wenk" <lpwenk <@t> sbcglobal.net>
Subject: RE: [Histonet] Specimen Rejection - QA
To: "'Amy Self'" <ASelf <@t> georgetownhospitalsystem.org>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000501c873b5$faa43740$0202a8c0 <@t> HPPav2>
Content-Type: text/plain;	charset="us-ascii"

Thought this might be a start.

Identification errors involving clinical laboratories: A College of American
Pathologists Q-Probes Study of Patients and Specimen Identification Errors
at 120 Institutions

College of American Pathologists; Valenstein PN, Raab SS, Walsh MK

Arch Pathol Lab Med. 2006 Aug;130(8):1106-13. 

Breaks it down into identification errors:
- Primary specimen label error
- Initial registration/order entry error
- Other clerical error
- Other reason for error
- Aliquot/block/slide label error
- Result entry error

So as Rene said, you need to narrow your project down (just accessioning
errors. Or just specimen container label errors. Or just slide label
errors.). Than write all the ways there could be errors, as well as ways to
identify WHO is doing the error. Such as for accessioning errors - what
information was wrong - patient's name? spelling error? Wrong name? Hospital
ID # - how was that wrong? Lab number - how was that wrong? Type of tissue -
how was that wrong? Etc. And which person accessioned in the information?

Peggy A. Wenk,HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amy Self
Sent: Tuesday, February 19, 2008 3:01 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Specimen Rejection - QA

 

Hello Histonetters,

 

 

I was wandering if anyone had any info or some kind of guidelines to help me
get started on a QA study especially for specimen rejection.
Thanks in advance,  Amy

 

 

 

Amy Self

Georgetown Hospital Systems

 

 



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------------------------------

Message: 2
Date: Wed, 20 Feb 2008 05:33:56 -0800
From: "Martin, Erin" <Erin.Martin <@t> ucsf.edu>
Subject: [Histonet] Pregnancy
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<DA617A88F598DE4888173E76E7E0783D9E286C <@t> EXVS07.net.ucsf.edu>
Content-Type: text/plain; charset=iso-8859-1

When I was pregnant last year, I talked to my nurse midwife about the chemicals and she referred me to a California pregnancy safety hotline.  I was doing gross and histo at the time.  They went over all of the chemicals with me and basically agreed with everything that Laurie said - reduce exposure as much as possible and work in well ventilated areas.  None of what I was working with is teratogenic (birth defect causing) but I was advised to be careful anyway.  Compromising my own health wouldn't be good for the baby, obviously.
 
Embedding and cutting are good options if you want to be extra careful.  I'm sure that no-one would want to ask a nervous mom-to-be to do anything that might be unhealthy for her or her baby.  It's not about legal accomodation, it's just the right thing to do.  Your HR office probably has some sort of guidelines to help you if you are not sure.  




------------------------------

Message: 3
Date: Wed, 20 Feb 2008 09:46:56 -0500
From: "Breckenridge, Sue" <sbreckenridge <@t> caperegional.com>
Subject: [Histonet] What is your experience with Histo-Clear II
To: <histonet <@t> pathology.swmed.edu>
Message-ID:
	<4D95C24EC0E4C84787A6919F1165B25E015B20B5 <@t> btmhems01.BTHOSP.INT>
Content-Type: text/plain;	charset="us-ascii"

I was hoping that any users (past or present) of the clearing agent
Histo-Clear II would be willing to give me feedback on how they liked
it. (For example, did it process tissues on par with other xylene
substitutes; Is there no adverse effect on Immunohistochemistry
staining; Can it be used for glass coverslipping & if so do the slides
dry in a reasonable amount of time). Thank-you for taking the time to
respond!

Sue Breckenridge, Histology Supervisor

Cape Regional Medical Center

Cape May Court House, NJ



------------------------------

Message: 4
Date: Wed, 20 Feb 2008 10:08:54 -0500
From: "McKnight, Tanisha" <tanisha.mcknight <@t> covance.com>
Subject: [Histonet] Formalin and Xylene Free Histology - Any Info
	about Glyo	Fixx
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<816E3C72F855F14985FC31D7C963AE6F04AEDF74 <@t> indexch03.ent.covance.com>
Content-Type: text/plain; charset="us-ascii"

Hello Again:
 
I am looking into Microwave processing and using Glyo Fixx as the fixative.
I am starting a histology lab, pretty much from scratch, but I have very
little experience in using Formalin and Xylene alternatives. The question I
have concerning the Glyo Fixx is whether or not I will need to use the same
safety precautions we use when grossing/handling specimens fixed in
formalin. Does it still need to be grossed under a hood? Are the ventilation
requirements different. 
 
If anyone here is using this, can you tell me how you handle this. 
 
 
Tanisha N. McKnight, HT (ASCP)




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------------------------------

Message: 5
Date: Wed, 20 Feb 2008 10:30:35 -0500
From: "Douglas D Deltour" <doug <@t> ppspath.com>
Subject: RE: [Histonet] What is your experience with Histo-Clear II
To: "'Breckenridge, Sue'" <sbreckenridge <@t> caperegional.com>,
	<histonet <@t> pathology.swmed.edu>
Message-ID: <1008597632-78010902 <@t> pathology.swmed.edu>
Content-Type: text/plain;	charset="us-ascii"

Sue,

I have been trying the xylene substitutes also. I have tried about ten
different brands and types of clearing reagent. First off, I can tell you
that none of them are superior to xylene. After that realization I can tell
you that the clearing agent that I chose was Citra-Clear from StatLab. It
seems to clear better and faster than the other alternative clearing
reagents (even Histo-Clear). If you are not fond of the citrus odor then the
runner-up is Formula 83 from CBG Biotech. After months of testing, these
were the top two alternative clearing reagents IMO. They are two different
types clearing reagents though. Citra-Clear is citrus based (D-Limonene) and
Formula 83 is a naphthenic hydrocarbon blend. Naphtha is refined petroleum
fractions.  

Douglas D. Deltour HT(ASCP)
Histology Manager
Professional Pathology Services, PC
One Science Court
Suite 200
Columbia, SC 29203
Office (803)252-1913
Fax (803)254-3262
Doug <@t> ppspath.com 
*****************************************************
PROFESSIONAL PATHOLOGY SERVICES, PC
NOTICE OF CONFIDENTIALITY
This message is intended only for the use of the individual or entity to
which it is addressed and may contain information that is privileged,
confidential and exempt from disclosure under applicable law. If the reader
of this message is not the intended recipient, you are hereby notified that
any dissemination, distribution, or copying of this communication is
strictly prohibited by law. If you have received this communication in
error, please notify me immediately.
 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Breckenridge, Sue
Sent: Wednesday, February 20, 2008 9:47 AM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] What is your experience with Histo-Clear II

I was hoping that any users (past or present) of the clearing agent
Histo-Clear II would be willing to give me feedback on how they liked
it. (For example, did it process tissues on par with other xylene
substitutes; Is there no adverse effect on Immunohistochemistry
staining; Can it be used for glass coverslipping & if so do the slides
dry in a reasonable amount of time). Thank-you for taking the time to
respond!

Sue Breckenridge, Histology Supervisor

Cape Regional Medical Center

Cape May Court House, NJ

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet






------------------------------

Message: 6
Date: Wed, 20 Feb 2008 10:37:57 -0500
From: "Douglas D Deltour" <doug <@t> ppspath.com>
Subject: RE: SPAM-LOW: [Histonet] Formalin and Xylene Free Histology -
	Any	Info about Glyo Fixx
To: "'McKnight, Tanisha'" <tanisha.mcknight <@t> covance.com>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<mailman.0.1203530401.4785.histonet <@t> lists.utsouthwestern.edu>
Content-Type: text/plain;	charset="us-ascii"

Tanisha,
My question is why would you want to use a Formalin alternative? I hope you
are talking about a research lab and are not concerned with validation of an
alternative fixative.  

Douglas D. Deltour HT(ASCP)
Histology Manager
Professional Pathology Services, PC
One Science Court
Suite 200
Columbia, SC 29203
Office (803)252-1913
Fax (803)254-3262
Doug <@t> ppspath.com 
*****************************************************
PROFESSIONAL PATHOLOGY SERVICES, PC
NOTICE OF CONFIDENTIALITY
This message is intended only for the use of the individual or entity to
which it is addressed and may contain information that is privileged,
confidential and exempt from disclosure under applicable law. If the reader
of this message is not the intended recipient, you are hereby notified that
any dissemination, distribution, or copying of this communication is
strictly prohibited by law. If you have received this communication in
error, please notify me immediately.
 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of McKnight,
Tanisha
Sent: Wednesday, February 20, 2008 10:09 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: SPAM-LOW: [Histonet] Formalin and Xylene Free Histology - Any Info
about Glyo Fixx

Hello Again:
 
I am looking into Microwave processing and using Glyo Fixx as the fixative.
I am starting a histology lab, pretty much from scratch, but I have very
little experience in using Formalin and Xylene alternatives. The question I
have concerning the Glyo Fixx is whether or not I will need to use the same
safety precautions we use when grossing/handling specimens fixed in
formalin. Does it still need to be grossed under a hood? Are the ventilation
requirements different. 
 
If anyone here is using this, can you tell me how you handle this. 
 
 
Tanisha N. McKnight, HT (ASCP)




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------------------------------

Message: 7
Date: Wed, 20 Feb 2008 07:41:44 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] What is your experience with Histo-Clear II
To: "Breckenridge, Sue" <sbreckenridge <@t> caperegional.com>,
	histonet <@t> pathology.swmed.edu
Message-ID: <841403.32979.qm <@t> web61219.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Several postings on Histonet about Histoclear can be summarized as follows:
  1- hardens brain, liver and spleen
  2- not very good for coverslipping
  3- poor dewaxing agent
  4- not compatible with xylene/toluene based mounting media
  5- reported as causing skin problems and headaches
  6- fades hematoxylin.
  For more information consult Histonet archives under "Histoclear"
René J.

"Breckenridge, Sue" <sbreckenridge <@t> caperegional.com> wrote:
  
 



       
---------------------------------
Never miss a thing.   Make Yahoo your homepage.

------------------------------

Message: 8
Date: Wed, 20 Feb 2008 11:13:43 -0600
From: "Nita Searcy" <NSEARCY <@t> swmail.sw.org>
Subject: [Histonet] Dako Info
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s7bc0b6d.095 <@t> swmail.sw.org>
Content-Type: text/plain; charset=US-ASCII

Posting for Lead Immunohistochemistry Technologist:

Anyone using Dako Envision & Detection system heard about it being
discontinued? What substitutions are you considering?





------------------------------

Message: 9
Date: Wed, 20 Feb 2008 09:27:48 -0800
From: Patti Loykasek <ploykasek <@t> phenopath.com>
Subject: Re: [Histonet] Dako Info
To: Nita Searcy <NSEARCY <@t> swmail.sw.org>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <C3E1A314.12F8E%ploykasek <@t> phenopath.com>
Content-Type: text/plain;	charset="US-ASCII"

I think I recall hearing that the Envision, not Envision+, was being
discontinued, but I am not 100% positive.

Patti Loykasek



> Posting for Lead Immunohistochemistry Technologist:
> 
> Anyone using Dako Envision & Detection system heard about it being
> discontinued? What substitutions are you considering?
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 



This e-mail message, including any attachments, is for the sole use of the 
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recipient, please contact the sender by e-mail and destroy all copies of the 
original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. 
at (206) 374-9000.




------------------------------

Message: 10
Date: Wed, 20 Feb 2008 09:38:35 -0800
From: Patti Loykasek <ploykasek <@t> phenopath.com>
Subject: [Histonet] IHC Detection systems
To: histonet <histonet <@t> pathology.swmed.edu>
Message-ID: <C3E1A59B.12F92%ploykasek <@t> phenopath.com>
Content-Type: text/plain;	charset="US-ASCII"

Hi all. We are currently doing some preliminary evaluation of IHC  small
polymer detection systems. We currently use Envision+ detection. I was
hoping others who have experience with the newer detections would be willing
to share some insights - both positive and negative. I would be willing to
provide feedback as we evaluate. Thank you.



Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA




This e-mail message, including any attachments, is for the sole use of the 
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recipient, please contact the sender by e-mail and destroy all copies of the 
original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. 
at (206) 374-9000.




------------------------------

Message: 11
Date: Wed, 20 Feb 2008 12:40:34 -0500
From: Dana Settembre <settembr <@t> umdnj.edu>
Subject: Re: [Histonet] Dako Info
To: histonet <@t> lists.utsouthwestern.edu,	Patti Loykasek
	<ploykasek <@t> phenopath.com>,	Nita Searcy <NSEARCY <@t> swmail.sw.org>
Message-ID: <s7bc1fc9.069 <@t> smtpnpc.umdnj.edu>
Content-Type: text/plain; charset=US-ASCII

Patti is correct.  I just spoke to Dako Tech who said that the Envision
+ will not be.
They already discont. their "standard Envision"


Dana Settembre, HT ASCP
Immunohistochemistry Lab
UMDNJ - University Hospital
Newark, NJ    USA


>>> Patti Loykasek <ploykasek <@t> phenopath.com> 02/20/08 12:27 PM >>>
I think I recall hearing that the Envision, not Envision+, was being
discontinued, but I am not 100% positive.

Patti Loykasek



> Posting for Lead Immunohistochemistry Technologist:
> 
> Anyone using Dako Envision & Detection system heard about it being
> discontinued? What substitutions are you considering?
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 
> 



This e-mail message, including any attachments, is for the sole use of
the 
intended recipients and may contain privileged information. Any
unauthorized 
review, use, disclosure or distribution is prohibited. If you are not
the intended 
recipient, please contact the sender by e-mail and destroy all copies
of the 
original message, or you may call PhenoPath Laboratories, Seattle, WA
U.S.A. 
at (206) 374-9000.


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------------------------------

Message: 12
Date: 20 Feb 2008 09:47:44 -0800
From: daliaelrouby <@t> hotmail.com
Subject: [Histonet] Join dalia el-rouby on Yahoo! Messenger!
To: histonet <@t> pathology.swmed.edu
Message-ID: <1008589448-78503137 <@t> pathology.swmed.edu>
Content-Type: text/plain; charset=en_US.ISO-8859-1


dalia el-rouby wants to talk with you using Yahoo! Messenger:





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------------------------------

Message: 13
Date: Wed, 20 Feb 2008 12:49:33 -0500
From: "Weems, Joyce" <JWEEMS <@t> sjha.org>
Subject: RE: [Histonet] Dako Info
To: "Patti Loykasek" <ploykasek <@t> phenopath.com>,	"Nita Searcy"
	<NSEARCY <@t> swmail.sw.org>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<1CD6831EB9B26D45B0A3EAA79F7EBD320518E70E <@t> sjhaexc02.sjha.org>
Content-Type: text/plain;	charset="us-ascii"

Correct. 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patti
Loykasek
Sent: Wednesday, February 20, 2008 12:28 PM
To: Nita Searcy; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Dako Info

I think I recall hearing that the Envision, not Envision+, was being
discontinued, but I am not 100% positive.

Patti Loykasek



> Posting for Lead Immunohistochemistry Technologist:
> 
> Anyone using Dako Envision & Detection system heard about it being 
> discontinued? What substitutions are you considering?
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 



This e-mail message, including any attachments, is for the sole use of
the intended recipients and may contain privileged information. Any
unauthorized review, use, disclosure or distribution is prohibited. If
you are not the intended recipient, please contact the sender by e-mail
and destroy all copies of the original message, or you may call
PhenoPath Laboratories, Seattle, WA U.S.A. 
at (206) 374-9000.


_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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------------------------------

Message: 14
Date: Wed, 20 Feb 2008 12:57:25 -0500
From: "Lynne Cates" <lcates <@t> synecor.com>
Subject: [Histonet] Adhesive tape & MMA
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<A0364DF9F7B03A499C5BE6D3FB343E60913AA9 <@t> synecor-main.CORP.synecor.local>
	
Content-Type: text/plain; charset="us-ascii"

Does anyone have any experience using scotch tape staining, adherence to
slide on MMA sections? 

Thanks in advance.

 

Lynne Cates, HT (ASCP)

Supervisor Histopathology Services

SyneCor, LLC

3908 Patriot Drive

Suite 170

Durham, NC 27703

Tel (919) 541-9977 X 119

Fax (919) 541-9975

 


------------------------------

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