[Histonet] quenching of Fluorescent signal

[Sally Price]

Ann:
I've had exceptionally good results with Biocare's anti-mouse and
anti-rabbit Dylight-488 followed by DAP, and the signal lasts for weeks.
Biocare also offers a product called FluoroCare 'anti-fade' medium
Good Luck!
-- 
Sally
------------------------------

Message: 17
Date: Tue, 19 Feb 2008 18:53:56 -0500 (EST)
From: "FU,DONGTAO" <fudo <@t> ufl.edu>
Subject: [Histonet] quenching of Fluorescent signal
To: Histonet <@t> lists.utsouthwestern.edu

Hi, all
I am doing fluorescent staining on fresh frozen human spleen
using CD markers, like CD4, CD8, etc these days. The secondary
antibody I used is AF 488, the mount media was DAPI(hard set) from
vector. I can get very nice staining, however, I find these
signals only can be kept for a couple of days, then start to
quench deeply, although I put my stained slides in 4C. Does anyone
have experience on how to keep fluorecent signal last longer? And
usually how long do you still get your IF signal after mounting?

Thank you,
Ann Dongtao Fu MD, Ph.D
Lab Manager
Dept. of Pathology
Lab phone: 352-273-7752
Lab FAX: 352-273-7755
Lab address: D11-50
PO Box: 100275
1600 SW Archer Road
University of Flodrida
Gainesville, FL 32610