[Histonet] quenching of Fluorescent signal

[FU,DONGTAO]

Hi, all

  I am doing fluorescent staining on fresh frozen human spleen 
using CD markers, like CD4, CD8, etc these days. The secondary 
antibody I used is AF 488, the mount media was DAPI(hard set) from 
vector. I can get very nice staining, however, I find these 
signals only can be kept for a couple of days, then start to 
quench deeply, although I put my stained slides in 4C. Does anyone 
have experience on how to keep fluorecent signal last longer? And 
usually how long do you still get your IF signal after mounting?

  Thank you,

Ann Dongtao Fu MD, Ph.D
Lab Manager
Dept. of Pathology
Lab phone: 352-273-7752
Lab FAX: 352-273-7755
Lab address: D11-50
PO Box: 100275
1600 SW Archer Road
University of Flodrida
Gainesville, FL 32610