AW: [Histonet] Sudan III

[Gudrun Lang]

We prepare our Sudan III solution in Aceton/70% ethanol 1:1 and add as much
dye until saturation. Let stand for a few days, filter before use. Allways
close tightly because of the aceton-vapor.

Gudrun Lang
 
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754
-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Sonny
Duong
Gesendet: Montag, 18. Februar 2008 18:01
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] Sudan III

Hi all!

I am attempting to perform fecal fat microscopy with Sudan 
III stain.  My protocol requires me to have 1% Sudan III 
dye, and my question is, 1% in what?  The dye is insoluble 
in water, so what should I dissolve the stain in?  Ehtanol? 
Chloroform??

Thanks,

Sonny Duong

Carla Green Lab
University of Virginia


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