[Histonet] RE: Suggestions for RCC and Caldesmon antibodies
Liz Chlipala
liz <@t> premierlab.com
Mon Feb 18 10:39:00 CST 2008
Yudi
We get our caldesmon from:
Caldesmon Santa Cruz sc-52991 SPM108
We have only used it in canine tissue, but it works well.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
Ship to Address:
Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bolig,
Yuriko Durst
Sent: Monday, February 18, 2008 9:01 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Suggestions for RCC and Caldesmon antibodies
Can anyone suggest which antibodies to use for RCC and Caldesmon? We
have tried Dako's RCC and want to try another company. The Caldesmon is
new for us, so any suggestion is greatly appreciated.
Thank you for your time!
Yudi Bolig
-----Original Message-----
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histonet-request <@t> lists.utsouthwestern.edu
Sent: Sunday, February 17, 2008 1:01 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 51, Issue 26
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Today's Topics:
1. Iron control (Cheryl Crowder)
2. Help with FISH on urine samples... (Jennifer Easterling)
3. Re: Help with FISH on urine samples... (Rene J Buesa)
4. RE: GMS and Bouin's fixation (Lee & Peggy Wenk)
5. AW: [Histonet] Help with FISH on urine samples... (Gudrun Lang)
6. Scabies (Rathborne, Toni)
----------------------------------------------------------------------
Message: 1
Date: Sat, 16 Feb 2008 12:40:02 -0600
From: "Cheryl Crowder" <ccrowder <@t> vetmed.lsu.edu>
Subject: [Histonet] Iron control
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <WorldClient-F200802161240.AA40020787 <@t> vetmed.lsu.edu>
Content-Type: text/plain; charset="us-ascii"
If your looking for a gorgeous iron positive control try a rabbit liver.
They have free iron and stain beautifully with all iron stains.
Cheryl
Cheryl Crowder, BA, HTL(ASCP)
Chief Technologist
Anatomic Pathology
Department of Pathobiological Sciences
School of Veterinary Medicine
Louisiana State University
Skip Bertman Drive
Baton Rouge, LA 70803
225-578-9734
FAX: 225-578-9720
------------------------------
Message: 2
Date: Sat, 16 Feb 2008 11:46:40 -0800 (PST)
From: Jennifer Easterling <yellowstar237 <@t> yahoo.com>
Subject: [Histonet] Help with FISH on urine samples...
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <561398.92942.qm <@t> web39814.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hello everyone, I am new to this list and I have found it very helpful.
Hopefully, someone can help me with my problem...There is a lab in my
area wanting to start FISH on urine samples for bladder cancer. They are
in the very beginning stages and have no policy or procedure manual in
place. If anyone has any information on either of these two things, any
help would be greatly appreciated.
Thanks in advance,
Jennifer
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Message: 3
Date: Sat, 16 Feb 2008 13:00:04 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Help with FISH on urine samples...
To: Jennifer Easterling <yellowstar237 <@t> yahoo.com>,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <96706.87445.qm <@t> web61211.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Jennifer:
Try to contact a cytotechnologist in your area and s/he will tell you
how to prepare cytospins from urine. With the cytospins you can prepare
slides to treat with FISH for the cancer probes you want to use.
Ren? J.
Jennifer Easterling <yellowstar237 <@t> yahoo.com> wrote:
Hello everyone, I am new to this list and I have found it very
helpful. Hopefully, someone can help me with my problem...There is a lab
in my area wanting to start FISH on urine samples for bladder cancer.
They are in the very beginning stages and have no policy or procedure
manual in place. If anyone has any information on either of these two
things, any help would be greatly appreciated.
Thanks in advance,
Jennifer
---------------------------------
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it now.
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Message: 4
Date: Sat, 16 Feb 2008 18:43:27 -0500
From: "Lee & Peggy Wenk" <lpwenk <@t> sbcglobal.net>
Subject: RE: [Histonet] GMS and Bouin's fixation
To: "'Paul Bradbury'" <histology.bc <@t> shaw.ca>, <alineumann <@t> aol.com>,
"'HistoNet Server'" <histonet <@t> pathology.swmed.edu>
Message-ID: <000701c870f5$baa5b6e0$0202a8c0 <@t> HPPav2>
Content-Type: text/plain; charset="us-ascii"
The reasoning sounds right to me.
I might suggest using 0.5% periodic acid (such as in the PAS stain) for
5-10 minutes as the oxidizer for the GMS stain, instead of the chromic
acid.
Since the picric acid has started the carbohydrate oxidation, and the
chromic acid is then overoxidizing the remaining carbohydrates, then
using a mild oxidizer such as periodic acid might do the trick. Usually,
periodic acid is NOT used in the GMS stain, as not enough background is
overoxidized.
The PASM/Jones stain used to demonstrate basement membranes in kidney
biopsies is the same silver methenamine solution as in the GMS, but
periodic acid is used instead of chromic acid. Our kidney biopsies are
fixed in DB, which is an alcoholic-Bouins, which does contain picric
acid. But the PASM/Jones stain continues to demonstrate basement
membrane. If a GMS is done on the kidney biopsy, the basement membrane
is not demonstrated.
So I think using 0.5% periodic acid for 5-10 minutes, rinsing in d.
water, and then going into the GMS methenamine silver solution should
work.
Peggy A. Wenk, HTL(ASC)SLS
William Beaumont Hospital
Royal Oak, MI 48073
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paul
Bradbury
Sent: Saturday, February 16, 2008 2:53 AM
To: alineumann <@t> aol.com; HistoNet Server
Subject: [Histonet] GMS and Bouin's fixation
Hi Alice,
Logically, there may be a conflict between fixing tissues in Bouin's and
successful demonstration of fungi using a methemamine silver technique.
This is my reasoning:
Bouin has long been the recommended fixative for glycogen and
proteoglygans which are best demonstrated by the PAS reaction. In the
PAS, carbohydrate groups are oxidised by periodic acid to form
aldehydes, which in turn react with Schiff reagent to produce the
magenta colour. Periodic acid, as a 1% solution at room temperature,
will oxidize these groups only as far as the aldehyde stage.
The methenamine silver is essentially a modification of that same
concept, but using chromic acid and an unstable silver solution in place
of the periodic acid and Schiff reagent. In the methenamine silver
technique, the carbohydrates in the capsule surrounding the fungal
elements are oxidized to form aldehydes which reduce the silver solution
to produce visible deposits of silver. Prolonged treatment with chromic
acid will over-oxidize the carbohydrates to carboxyl groups which are
non-reactive with the silver solution.
There may well be a reaction between the picric acid in Bouin's fixative
and carbohydrates. Picric acid is a potent oxidizer and may begin the
oxidation of the carbohydrate groups in the fungal capsule. When the
sections are further oxidized by chromic acid, the fungal walls become
over-oxidized and form non-reactive carboxyl groups.
It may be worth trying a much shorter chromic acid treatment on Bouin's
fixed tissues to see if this will leave the carbohydrates in the fungi
at the aldehyde stage.
Most methenamine silver techniques suggest a 60 minutes treatment in 5%
chromic acid at room temperature. In the case of Bouin-fixed tissues, I
would suggest running a trial using a range of chromic acid times from
10-40 minutes to see if the fungi are still reactive with one of the
shorter oxidation times.
I would be very interested to know if this solves your problem.
Paul Bradbury
Kamloops, BC
Canada
alineumann <@t> aol.com wrote:
> Hello all, has anyone ever experienced Bouin's fixed tissue?preventing
> the methenamine from staining fungus?? Thank you,
>
>
> Alice Neumann MD
> Western Wyoming Pathology
> Jackson, WY 83001
>
> Cell: 307-413-4042
> Work: 307-733-6418
> Home: 307-734-4410
> Fax: 307-734-0885
>
> ______________________________________________________________________
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------------------------------
Message: 5
Date: Sun, 17 Feb 2008 12:18:03 +0100
From: "Gudrun Lang" <gu.lang <@t> gmx.at>
Subject: AW: [Histonet] Help with FISH on urine samples...
To: "'Jennifer Easterling'" <yellowstar237 <@t> yahoo.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <001e01c87156$c3c06070$eeeea8c0 <@t> dielangs.at>
Content-Type: text/plain; charset="iso-8859-1"
I think, this site could help you.
http://www.urovysion.com/proceduraloverview_358.aspx
Gudrun Lang
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754
-----Urspr?ngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von
Jennifer Easterling
Gesendet: Samstag, 16. Februar 2008 20:47
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] Help with FISH on urine samples...
Hello everyone, I am new to this list and I have found it very helpful.
Hopefully, someone can help me with my problem...There is a lab in my
area wanting to start FISH on urine samples for bladder cancer. They are
in the very beginning stages and have no policy or procedure manual in
place. If anyone has any information on either of these two things, any
help would be greatly appreciated.
Thanks in advance,
Jennifer
---------------------------------
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it now.
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------------------------------
Message: 6
Date: Sun, 17 Feb 2008 12:24:28 -0500
From: "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com>
Subject: [Histonet] Scabies
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E78340C766A5284D999F5F5891DDF89007D16D79 <@t> smcmail.somerset-healthcare.co
m>
Content-Type: text/plain; charset="utf-8"
Hi all!
I have a question about the proper way to process/prepare skin scrapings
for scabies. We have had a few cases in the last year, and want to be
sure that they are being submitted properly. Also, does anyone know
where we might purchase a positive slide/picture to identify the eggs.
With something that occurs so infrequently, the pathologist's would like
to have a reference so that they know what they are looking for.
Thanks,
Toni
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