[Histonet] PAS + IF

[Amos Brooks]

Vinnie,
     That's the first thing I told her. She knows it, but still wants to do
it anyway. I think she plans to take images of the PAS after taking IF
images so that the architecture is the same. There may be some image
layering involved as well.

Thanks,
Amos

On Feb 15, 2008 10:23 AM, Della Speranza, Vinnie <dellav <@t> musc.edu> wrote:

> Amos, do you or the researcher know that basic fuchsin or its constituent
> dyes (rosanilin, pararosaniline) will not fluoresce with the incident
> excitation wavelength that will be used for the fluorescent tag? Aside from
> the issues you raised this could make for interpretation difficulties
>
> Vinnie Della Speranza
>
> Manager for Anatomic Pathology Services
>
> 165 Ashley Avenue  Suite 309
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> Charleston, South Carolina 29425
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>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amos Brooks
> Sent: Thursday, February 14, 2008 9:36 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] PAS + IF
>
> Hi,
>     There is a researcher here that is interested in doing a PAS and an
> immunofluorescent label on the same slide. She is using formalin fixed
> paraffin embedded tissue and the antibody requires heat induced epitope
> retrieval. So obviously the PAS will need to come first as the reagents
> involved would innevitably destroy the fluorescent label. The question is:
> will the retrieval affect the PAS reaction? My initial guess was that the
> PAS is a fairly strong bond (spill schiffs on your hand ... see what I
> mean!) so it should survive, but that is just a guess. Has anyone tried
> it?
>
> Thanks,
> Amos Brooks
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