[Histonet] PTAH on frozen sections

[Rene J Buesa]

Just make absolutely sure that your sections are not going to fall. You could try to coat the slides with Mayer's albumin or with Elmer's glue. Let the slides with the coat dry in an oven overnight before using them.
  Put you FS on the coated slides and air dry them. Fix them afterwards in 10% NBF for 3-5 minutes and wash in water to eliminate the OCT and stain them afterwards with the PTAH. Do NOT use any microwave shortcut, but stain the sections in the PTAH overnight.
  Perhaps they will survive. Your problem will be with the sections adherence.
  René J.

"Molinari, Betsy" <BMolinari <@t> heart.thi.tmc.edu> wrote:
  Happy Friday!
Is there any change I should make in my PTAH protocol when staining
frozen sections?
Thanks!
Betsy

Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave
MC1-283
Houston,TX 77030-2607
832-355-6524
832-355-6812

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




       
---------------------------------
Never miss a thing.   Make Yahoo your homepage.