[Histonet] Double-staining with same secondary antibody?

[Rene J Buesa]

I think you will have no problems since your secondary can react with goat and mouse produced antibodies. The fact that the 1st requires HIER and the secondary does not is no problem either, the antigenic sites will be available for both.
  Using DAB-Nickel will give you the two colors you will need to distinguish both complexes (se 2ry Ab with conventional DAB).
  You should try it as described without buying any other reagents firts.
  René J.

Min-Han Tan <minhan.tan <@t> gmail.com> wrote:
  
 



       
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