[Histonet] vimentin
Melissa Mazan
melissa.mazan <@t> tufts.edu
Wed Feb 13 11:03:50 CST 2008
We've used bd biosciences mouse monoclonal 550513 - 1:100 on FFPE lung
tissue overnight at 4C with Alexafluor secondary for 30 min at 37C.
We've also used the abcam chicken vimentin - works pretty well but huge
background on westerns unless you use nitrocellulose. Melissa
histonet-request <@t> lists.utsouthwestern.edu wrote:
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> Today's Topics:
>
> 1. Re: Used equipment (Renko, Heather D.)
> 2. ? vimentin IHC ab from ABR bioreagents (Kathy Cormier)
> 3. H & E stainers (Hineline, Paula)
> 4. Region III Meeting Program (Shirley Powell)
> 5. Re: H & E stainers (Rene J Buesa)
> 6. Biopsy bags (Martin, Erin)
> 7. RE: Biopsy bags (Horn, Hazel V)
> 8. Coverslipping with the Dako Autostainer (Sate Hamza)
> 9. RE: Biopsy bags (Mike Pence)
> 10. little things in histology (WWmn916 <@t> aol.com)
> 11. a reference for Streptavidin-biotin detecting system (K M)
> 12. RE: Xylene Resistant Floors (Kim Merriam)
> 13. Re: Coverslipping with the Dako Autostainer (Rene J Buesa)
> 14. H&E stainer (Blazek, Linda)
> 15. Re: little things in histology (Rene J Buesa)
> 16. Re: a reference for Streptavidin-biotin detecting system
> (Rene J Buesa)
> 17. RE: Xylene Resistant Floors (Rene J Buesa)
> 18. Re: H&E stainer (Cynthia Robinson)
> 19. Double-staining with same secondary antibody? (Min-Han Tan)
> 20. In-service on Ht exam (Cheri Miller)
> 21. Ethanol preserved tissues (Laura Hunt)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Tue, 12 Feb 2008 12:04:20 -0600
> From: "Renko, Heather D." <Heather.D.Renko <@t> osfhealthcare.org>
> Subject: [Histonet] Re: Used equipment
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <40026EDDE64CDA47AB382C52619ACD3C0A09DD2B <@t> pmc-rfd-mx01.intranet.osfnet.org>
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> Content-Type: text/plain; charset=iso-8859-1
>
> I have a Microm 760X H&E stainer that we are replacing. If you know of anyone that might want it or even for parts email me directly.
>
> Heather D. Renko, Histology Coordinator
> OSF Saint Anthony Medical Center
> 5666 East State Street
> Rockford, Illinois 61108
> Direct: 815-395-5410
> Heather.D.Renko <@t> osfhealthcare.org
>
>
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> ------------------------------
>
> Message: 2
> Date: Tue, 12 Feb 2008 14:21:41 -0500
> From: "Kathy Cormier" <cormier <@t> MIT.EDU>
> Subject: [Histonet] ? vimentin IHC ab from ABR bioreagents
> To: <histonet <@t> pathology.swmed.edu>
> Message-ID: <000a01c86dac$7f5fb3c0$92003712 <@t> mit.edu>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hi All,
>
> Has anyone tried the vimetin mouse mono ab from ABR bioreagents? I need to find a vimentin that will work on FFPE mouse tissue, and I do not want to spend $300 if the ab does not work well.... Any other ab that anyone knows works well? (yes, I checked the archives!)
>
> Thanks!
>
> Kathy
> DCM MIT
>
> ------------------------------
>
> Message: 3
> Date: Tue, 12 Feb 2008 14:02:29 -0600
> From: "Hineline, Paula" <phineline <@t> grandpath.com>
> Subject: [Histonet] H & E stainers
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <ABD957D5CDBB014F96C0502AA85EBFAD022890D3 <@t> cpsrv09.cunninghampathology.com>
>
> Content-Type: text/plain; charset="us-ascii"
>
>
> Hello,
> We are researching histology H&E stainers for our lab. We have gotten
> quotes on the Leica ST 5020 multistainer and the Leica ST 4040 linear
> stainer. Recommendations would be appreciated.
> Thanks
> Paula Hineline, HT ASCP
> Pathology Laboratory
> phineline <@t> grandpath.com
>
>
>
>
> ------------------------------
>
> Message: 4
> Date: Tue, 12 Feb 2008 15:17:13 -0500
> From: Shirley Powell <POWELL_SA <@t> Mercer.edu>
> Subject: [Histonet] Region III Meeting Program
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <01MR7OTAYQ76002EXU <@t> Macon2.Mercer.edu>
> Content-Type: text/plain; charset=us-ascii
>
> Hi Guys,
>
> The final program for the 2008 Region III Symposium/Convention hosted by GSH
> is up on the website at www.histosearch.com/gsh in a .pdf and .doc format,
> as well as vendor registration forms. Print out you program and mail it in
> soon. The hotel reservation deadline is March 3rd, so make them soon.
>
> Thanks,
> Shirley Powell, GSH Secretary/Registrar
>
>
>
>
> ------------------------------
>
> Message: 5
> Date: Tue, 12 Feb 2008 12:41:32 -0800 (PST)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] H & E stainers
> To: "Hineline, Paula" <phineline <@t> grandpath.com>,
> histonet <@t> lists.utsouthwestern.edu
> Message-ID: <477774.54198.qm <@t> web61222.mail.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Get quotes (and ask for a demo) of the Sakura H&E stainer.
> René J.
>
> "Hineline, Paula" <phineline <@t> grandpath.com> wrote:
>
> Hello,
> We are researching histology H&E stainers for our lab. We have gotten
> quotes on the Leica ST 5020 multistainer and the Leica ST 4040 linear
> stainer. Recommendations would be appreciated.
> Thanks
> Paula Hineline, HT ASCP
> Pathology Laboratory
> phineline <@t> grandpath.com
>
>
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> ------------------------------
>
> Message: 6
> Date: Tue, 12 Feb 2008 13:23:51 -0800
> From: "Martin, Erin" <Erin.Martin <@t> ucsf.edu>
> Subject: [Histonet] Biopsy bags
> To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <DA617A88F598DE4888173E76E7E0783D9E285F <@t> EXVS07.net.ucsf.edu>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hi everyone.
>
> Does anyone have an inexpensive source for the nylon biosy bags? The price quote we got from Thermo Shandon was about $2/bag - yikes!!! - for the small size. I hope that there is something cheaper out there...
>
> Thanks in advance!
>
> Erin Martin
>
>
>
>
> ------------------------------
>
> Message: 7
> Date: Tue, 12 Feb 2008 15:31:02 -0600
> From: "Horn, Hazel V" <HornHV <@t> archildrens.org>
> Subject: RE: [Histonet] Biopsy bags
> To: "Martin, Erin" <Erin.Martin <@t> ucsf.edu>, "histonet"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82B12 <@t> EMAIL.archildrens.org>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi Erin,
> I found the old fashioned paper tea bags at Sunburst Bottle Company of
> Sacremento for only 5 cents each. The phone number is 916-929-3604.
> The cat# is tb 1
>
>
> Hazel Horn
> Hazel Horn, HT/HTL (ASCP)
> Supervisor of Histology
> Arkansas Children's Hospital
> 800 Marshall Slot 820
> Little Rock, AR 72202
>
> phone 501.364.4240
> fax 501.364.3912
>
> visit us on the web at: www.archildrens.org
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Martin,
> Erin
> Sent: Tuesday, February 12, 2008 3:24 PM
> To: histonet
> Subject: [Histonet] Biopsy bags
>
> Hi everyone.
>
> Does anyone have an inexpensive source for the nylon biosy bags? The
> price quote we got from Thermo Shandon was about $2/bag - yikes!!! - for
> the small size. I hope that there is something cheaper out there...
>
> Thanks in advance!
>
> Erin Martin
>
>
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> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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>
>
> ------------------------------
>
> Message: 8
> Date: Tue, 12 Feb 2008 15:41:11 -0600
> From: "Sate Hamza" <dermpathsy <@t> gmail.com>
> Subject: [Histonet] Coverslipping with the Dako Autostainer
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <8854ff80802121341i1204884ar376df31d12843ad4 <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Colleagues,
>
> We use the DakoCytomation Autostainer and we are wondering what
> coverslipping instruments are being used to cover the slides with Dako slide
> labels ..
>
> Thank you ..
>
> Sate
>
>
--
Melissa R. Mazan, DVM, Diplomate ACVIM
Associate Professor and Director of Sports Medicine
Tufts Cummings School of Veterinary Medicine
200 Westboro Road
North Grafton, MA 01536
508-887-4589
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