[Histonet] Ethanol preserved tissues
Laura Hunt
lhunt <@t> uta.edu
Wed Feb 13 10:50:08 CST 2008
Hi Histo folks,
So I have a friend you is interested in doing some histology on snake
embryos. Unfortunately, the supply of this species of snake embryos is
low and the only ones currently available have been museum specimens.
Thus, they have been stored in ethanol for awhile. Are these hopeless
or is there anyway to carry out some histology on them?
Just wondering,
Laura Hunt
On Feb 12, 2008, at 12:00 PM, histonet-
request <@t> lists.utsouthwestern.edu wrote:
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> Today's Topics:
>
> 1. RE: prostate biopsy problem (Bell, Lynne)
> 2. re/prostate quesitons (Joyce Cline)
> 3. Re: re/prostate quesitons (Matt Bancroft)
> 4. Tennessee Society for Histotechnology - Call for Vendors
> (Hofecker, Jennifer L)
> 5. re/prostate questions (Joyce Cline)
> 6. $$$ Histology Position $$$ (April Sachau)
> 7. Phoenix AZ histotechs (Pathrm35 <@t> comcast.net)
> 8. RE: Histonet Digest, mouse pup euthanasia with CO2 (Jones, Lynne)
> 9. new prostate problem! (Nick and Amanda)
> 10. RELIA Histology Job Opportunity Alert 2/12/08 (Pam Barker)
> 11. Formalin recycler (Sharon.Davis-Devine)
> 12. RE: Formalin recycler (Laurie Colbert)
> 13. Opening for a Core Lab Manager (Henry, Charlene)
> 14. Re: new prostate problem! (pkromund <@t> gundluth.org)
> 15. Histology Core Lab Manager Position (Henry, Charlene)
> 16. Histology Core Lab Manager Position (Henry, Charlene)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Mon, 11 Feb 2008 13:17:17 -0500
> From: "Bell, Lynne" <Lynne.Bell <@t> hitchcock.org>
> Subject: RE: [Histonet] prostate biopsy problem
> To: "Joyce Cline" <jcline <@t> wchsys.org>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <ED658CF94165704EBE82F8D0D00A3F363E1954 <@t> CVMCNT1.CVMC.ORG>
>
> We had the same problem a few years ago. Do you use sponges in your
> cassette? We started using wet sponges instead of dry ones. We have
> not had a problem since.
>
> Lynne Bell, HT (ASCP)
> Central VT Hospital
> P. O. Box 547
> Barre, VT 05641
> 802-371-4923
>
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Mon, 11 Feb 2008 13:52:16 -0500
> From: "Joyce Cline" <jcline <@t> wchsys.org>
> Subject: [Histonet] re/prostate quesitons
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <000701c86cdf$392aeb40$1d2a14ac <@t> wchsys.org>
> Content-Type: text/plain; charset="us-ascii"
>
> 1. We do not have the same problem with our breast core biopsies.
> 2. We may have washing on block A and B but not on C,D or F and
> washing on E, H, etc.
> 3. The biopsies have no drying artifact on the edges.
> 4. We use a low temp wax, Blue Ribbon paraffin for processing and
> embedding. Our processor is set at 57.
> 5. The tissue sections fall off during H&E staining.
> 6. We do not use sponges, we use screened cassettes.
> 7. The Stay on is used alone, not with the charged slides.
>
> Our pathologist that is our prostate specialist thinks each one of
> us is
> doing something different when we cut. We have all discussed this
> matter
> and can find no
> differences when we cut.
>
>
> ***** CONFIDENTIALITY NOTICE *****
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> for
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>
>
> ------------------------------
>
> Message: 3
> Date: Mon, 11 Feb 2008 11:17:43 -0800 (PST)
> From: Matt Bancroft <mohs76009 <@t> yahoo.com>
> Subject: Re: [Histonet] re/prostate quesitons
> To: Joyce Cline <jcline <@t> wchsys.org>, histonet <@t> lists.utsouthwestern.edu
> Message-ID: <446182.81960.qm <@t> web63409.mail.re1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> The issue that I have found is even when one uses + charged slides,
> you still need to make sure that all the water is gone from under
> the tissue section. When I cut needle biopsies (kidney, prostate,
> or breast) after I get the section on the slide I take my forceps
> tare a little slit in the corner of my sections and use a kim wipe
> to absorb the water. I have not had a problem with sections falling
> off since I started doing this. I also use HISTOBOND slides from
> StatLab medical products. These slides are very sticky (sometimes to
> much).
>
> Matt Bancroft HT(ASCP)
> Laboratory Manager
>
>
> Joyce Cline <jcline <@t> wchsys.org> wrote:
> 1. We do not have the same problem with our breast core biopsies.
> 2. We may have washing on block A and B but not on C,D or F and
> washing on E, H, etc.
> 3. The biopsies have no drying artifact on the edges.
> 4. We use a low temp wax, Blue Ribbon paraffin for processing and
> embedding. Our processor is set at 57.
> 5. The tissue sections fall off during H&E staining.
> 6. We do not use sponges, we use screened cassettes.
> 7. The Stay on is used alone, not with the charged slides.
>
> Our pathologist that is our prostate specialist thinks each one of
> us is
> doing something different when we cut. We have all discussed this
> matter
> and can find no
> differences when we cut.
>
>
> ***** CONFIDENTIALITY NOTICE *****
> This message contains confidential information and is intended only
> for
> the individual named. If you are not the named addressee you should
> not
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> ---------------------------------
> Looking for last minute shopping deals? Find them fast with Yahoo!
> Search.
>
> ------------------------------
>
> Message: 4
> Date: Mon, 11 Feb 2008 13:25:00 -0600
> From: "Hofecker, Jennifer L" <jennifer.l.hofecker <@t> Vanderbilt.Edu>
> Subject: [Histonet] Tennessee Society for Histotechnology - Call for
> Vendors
> To: "histonet" <histonet <@t> lists.utsouthwestern.edu >
> Message-ID:
> <898D946569A27444B65667A49C0740520156449C <@t> mailbe06.mc.vanderbilt.edu>
> Content-Type: text/plain; charset="us-ascii"
>
> Happy Monday to all. I just wanted to take a moment and post a call
> for
> vendors. The TSH meeting is June 19-21 in Townsend, TN. Townsend is
> located in the scenic Smoky Mountain area. The meeting will be held at
> the valley view lodge. If you are interested in exhibiting at our
> meeting, please contact me for complete information. The deadline to
> have your company listed in the program as a sponsor is March 1.
>
> The official programs should be mailed by April 1 and I will post
> contact info again at that time for anyone interested in attending the
> meeting who may not receive a program.
>
> Thanks and have a great week!
>
>
> Jennifer L. Hofecker, HT(ASCP)
> Vanderbilt University Medical Center
> Division of Neuropathology
> Nashville, TN
> ph. (615)343-0083
> fax. (615)343-7089
> NSH Quality Control Committee Chair
> Tennessee Society for Histotechnology Secretary
> 2008 TSH Meeting Exhibit Coordinator
>
>
>
>
> ------------------------------
>
> Message: 5
> Date: Mon, 11 Feb 2008 14:31:41 -0500
> From: "Joyce Cline" <jcline <@t> wchsys.org>
> Subject: [Histonet] re/prostate questions
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <000701c86ce4$ba8c4f80$1d2a14ac <@t> wchsys.org>
> Content-Type: text/plain; charset="us-ascii"
>
> Our slides are dried vertically on plastic slide racks. I do not use
> Xylene, I use Formula 83.
>
>
> ***** CONFIDENTIALITY NOTICE *****
> This message contains confidential information and is intended only
> for
> the individual named. If you are not the named addressee you should
> not
> disseminate, distribute or copy this e-mail. Please notify the sender
> immediately by e-mail if you have received this e-mail by mistake and
> delete this e-mail from your system.
>
>
>
> ------------------------------
>
> Message: 6
> Date: Mon, 11 Feb 2008 14:06:16 -0600
> From: "April Sachau" <asachau <@t> titanmed.com>
> Subject: [Histonet] $$$ Histology Position $$$
> To: "Hofecker, Jennifer L" <jennifer.l.hofecker <@t> Vanderbilt.Edu>,
> "histonet" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>
> <7E3ACD48BA6E26408F3188FBF08693F7010D4C56 <@t> titansbs1.corp.titanmed.com>
> Content-Type: text/plain; charset="us-ascii"
>
> Hello Histonetters!
>
> I have an awesome position available in the Midwest. Great
> compensation
> package!!! It is a dayshift position for a minimum of 13 weeks.
> Possible permanent placement! Please let me know if you are
> interested
> or would like additional information!
>
>
> April Sachau
> Titan Medical Group
> Staff Supervisor
> Phone (866) 332-9600 Ext. 1023
> Fax (402) 332-5181
> asachau <@t> titanmed.com
>
> see us on the web at www.titanmed.com
>
>
>
> ------------------------------
>
> Message: 7
> Date: Mon, 11 Feb 2008 22:31:07 +0000
> From: Pathrm35 <@t> comcast.net
> Subject: [Histonet] Phoenix AZ histotechs
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <021120082231.3815.47B0CCAA000F06C100000EE72215551724CACC039D089B0EAF <@t> comcast.net
> >
>
> Content-Type: text/plain
>
> Could a histotech from the Phoenix, AZ area please contact me?
> Thanks,
> Ron Martin
>
> ------------------------------
>
> Message: 8
> Date: Mon, 11 Feb 2008 17:03:12 -0600
> From: "Jones, Lynne" <jonesly <@t> mir.wustl.edu>
> Subject: [Histonet] RE: Histonet Digest, mouse pup euthanasia with CO2
> To: "'histonet <@t> lists.utsouthwestern.edu'"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <D0674FCAA0917E439E7F2B65BEF2ED61014688E9D423 <@t> RAD-VMSRVEXV1.rad.wustl.edu
> >
>
> Content-Type: text/plain; charset="us-ascii"
>
> Hello to the list -
> I have little histology experience, but I've dealt with
> complications arising from both anesthesia and euthanasia in lab
> animals. Standard protocols don't work for every model.
>
> Neonatal rodents are very resistant to hypoxia (death can take up to
> 50 minutes, depending on age and strain). Our institutional policy
> is that neonatal rats and mice must remain inside the CO2 chamber
> for a minimum of 20 minutes after the gas flow is turned off if CO2
> is the sole agent used for euthanasia. Many Institutional Animal
> Care and Use Committees suggest consideration of alternative methods
> for neonates, or that CO2 anesthesia be followed by a physical method.
>
> FWIW, the paper below describes some of the biological effects
> associated with different euthanasia techniques.
> http://www.aclam.org/print/report_rodent_euth.pdf
>
> Based on the description of the brains, my suspicion is that the
> pups sat too long post-mortem before being fixed, or (going way out
> on a limb) that acidosis might have created problems if the formalin
> wasn't buffered.
>
> Lynne Jones
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu
> ] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
> Sent: Monday, February 11, 2008 12:07 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: Histonet Digest, Vol 51, Issue 17
>
> Send Histonet mailing list submissions to
> histonet <@t> lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
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> or, via email, send a message with subject or body 'help' to
> histonet-request <@t> lists.utsouthwestern.edu
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> histonet-owner <@t> lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
> 1. Fwd: [Histonet] Re: Mouse pups and processing (Greg Dobbin)
> 2. Verhoeff/ Trichome procedure (karenadams <@t> comcast.net)
> 3. RE: Verhoeff/ Trichome procedure (Fail, Mildred M.)
> 4. Iron staining (Till, Renee)
> 5. RE: OT: the weather (Ingles Claire)
> 6. prostate biopsy problem (Joyce Cline)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Mon, 11 Feb 2008 08:54:11 -0400
> From: "Greg Dobbin" <gvdobbin <@t> ihis.org>
> Subject: Fwd: [Histonet] Re: Mouse pups and processing
> To: <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <s7b00d3f.011 <@t> ihis.org>
> Content-Type: text/plain; charset=US-ASCII
>
>
>
> Greg Dobbin, R.T.
> Chief Technologist, Histology Lab
> Dept. of Laboratory Medicine,
> Queen Elizabeth Hospital,
> P.O. Box 6600
> Charlottetown, PE C1A 8T5
> Phone: (902) 894-2337
> Fax: (902) 894-2385
>
> There is some merit in doing the right thing rather badly,
> but absolutely none in doing the wrong thing excellently!
>
>>>> Greg Dobbin 2/11/2008 8:53 AM >>>
> Hi Pam,
> I participated on a project about 10 years ago where the rat pups were
> anesthetized using an overdose of halothane followed by exanguinationn
> (carotid and/or renal arteries). Here are the 2 relevant references:
> Comp Med. 2001 Apr;51(2):134-7.
> Histol Histopathol. 2002 Oct;17(4):1067-76.
> Good luck.
> Greg
>
>
> Greg Dobbin, R.T.
> Chief Technologist, Histology Lab
> Dept. of Laboratory Medicine,
> Queen Elizabeth Hospital,
> P.O. Box 6600
> Charlottetown, PE C1A 8T5
> Phone: (902) 894-2337
> Fax: (902) 894-2385
>
> There is some merit in doing the right thing rather badly,
> but absolutely none in doing the wrong thing excellently!
>
>>>> <pmarcum <@t> vet.upenn.edu> 2/10/2008 9:57 AM >>>
> Thank you Amos and I can appreciate your problem. Your discription of
> sqishy
> is
> prefect. I have also referred to the outer skin as lacy in appearance
> when
> attempting to section it. The other point I would make is the brain
> is
> mush
> with no form as if it is autolysized totally.
>
> I have tried splitting the abdomen and it does not help or is so
> minimal it
> doesn't help much. I have tried changing the processor and always
> process
> other tissues at the same time to show the PI it is not the reagents
> and
> paraffin. We have processed other pups before this over the last year
> and know
> they were not killed with CO2. They work great and section great (I
> still
> split
> the abdomen).
>
> I am at a loss and finally turned to all of you on HistoNet help. You
> are
> great
> and even though we may not have the answer yet we will I am sure of
> it.
> I know
> this is an ongoing problem for researchers with pups. Maybe with this
> CO2
> thing being a popular way for sacrificing animals we have hit on
> something that
> happens in pups and not adults. I had not really ever worried about
> it
> until
> now as I did my own sacrifice and it was fine. I wish they weren't so
> small I
> would attempt prefusion with a 2cc syringe and blunt needle as a last
> resort.
>
>
> I want to THANK everyone who has and will take the time to answer and
> help me
> with this issue. At least I can show the PI our lab is not the only
> one with
> the problem and we are all attempting to find a way out of it. I
> think
> from
> all I have seen we should be more aware of how the pups are sacrificed
> and note
> any differences between the way our PI's sacrifice them. Maybe all of
> the
> material submitted to the Histology area for research on pups and
> other
> animals
> should be recorded and we might see differences they don't in method.
> Recommendations from IACOCC do not take our area of histology into
> consideration as they are controlling the most humane and painless
> method of
> sacrificing animals over all. Perhaps one of us can get a paper out
> of
> this or
> a talk in the future that helps everyone.
>
> Who knows I even checked the phase of the moon this last time and it
> did not
> help.
>
> Thanks again and still looking. If I solve this you will all be teh
> first to
> know.
>
> Pam
>
> Quoting Amos Brooks <amosbrooks <@t> gmail.com>:
>
>> Pam,
>> We were having a similar problem with one of our researcher's P0
> mice.
>> I don't know how they sacrificed them, but they too dod not process
> at all.
>> They were squishy!
>> I reccommended they either bisect or at least make a good
> midline
>> incision prior to fixation. It still isn't perfect (is it ever?), but
> it
>> seems to be a bit better. This allows better solution penetration.
> Give it a
>> whirl and let us know how it goes.
>>
>> Amos
>>
>> Message: 13
>> Date: Fri, 08 Feb 2008 15:52:34 -0500
>> From: Pamela Marcum <pmarcum <@t> vet.upenn.edu>
>> Subject: [Histonet] Mouse pups and processing
>> To: Histonet <@t> lists.utsouthwestern.edu
>> Message-ID: <6.2.5.6.2.20080208154217.01c8a9b8 <@t> vet.upenn.edu>
>> Content-Type: text/plain; charset="us-ascii"; format=flowed
>>
>>
>>
>> I have been struggling with this for a while and need some help. We
>> currently have a project with 0 day mouse pups that are allowed to
> be
>> born normally and then sacrificed with CO2. We had several groups
>> earlier that were sacrificed a different way and they processed and
>> sectioned beautifully.
>>
>> These don't seem to fix well, dehydrate, clear or infiltrate worth a
>> darn. Since this is my first time using CO2 for sacrifice I need to
>> find out if it causes a problem or if I am just losing it. I have
>> not ever had this problem before and even re-processing does not
>> help. I know they are not infiltrating as they are floating in
>> paraffin at the end if I remove them from the processor to a vat of
>> paraffin. They will not sink. The pups are 1.2 to 1.3 grams each.
>>
>> If you can suggest a better way to sacrifice them please let me
>> know. Killing the mother and perfusing her is not an option as
> these
>> are not our mice. They are being given as favor so I am limited to
>> some extent.
>>
>> This was an overnight process with slightly altered alcohols to 45
>> minutes each, 1 xylene at 30 minutes and 2 Shandon Xylene Substitues
>> at 10 hour each to 4 paraffins at 45 X2 and 1 hour X2.
>>
>> Best Regards,
>>
>> Pamela A Marcum
>> Manager, Histology Special Procedures
>> University of Pennsylvania
>> School of Veterinary Medicine
>> R.S. Reynolds Jr. CORL
>> New Bolton Center
>> 382 West Street Road
>> Kennett Square, PA 19348
>>
>> Phone - 610-925-6278
>> Fax - 610-925-8120
>> E-mail - pmarcum <@t> vet.upenn.edu
>
>
> The materials in this message are private and may contain Protected
> Healthcare Information. If you are not the intended recipient, be
> advised that any unauthorized use, disclosure, copying or the taking
> of any action in reliance on the contents of this information is
> strictly prohibited. If you have received this email in error,
> please immediately notify the sender via telephone or return mail.
>
>
>
> ------------------------------
>
> Message: 9
> Date: Tue, 12 Feb 2008 20:30:24 +1300
> From: Nick and Amanda <nickandmanda <@t> paradise.net.nz>
> Subject: [Histonet] new prostate problem!
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <000d01c86d49$21db4ec0$c8084979 <@t> bow1>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hi!
>
> We have recently come up against a staining issue where our prostate
> cores have patches which are not staining. Our Pathologist tells us
> this is common in a particular High grade diagnosis so this is a
> problem when it is happening in all prostate cases.. It does not
> seem to be affecting other tissue staining (perhaps the odd one! no
> examples could be provided!! Could it possibly be drying out of the
> cores prior to being placed in formalin??
>
> Any Ideas welcome
>
> Just not sure!
> Wellington, NZ
>
> ------------------------------
>
> Message: 10
> Date: Tue, 12 Feb 2008 08:14:57 -0500
> From: "Pam Barker" <relia1 <@t> earthlink.net>
> Subject: [Histonet] RELIA Histology Job Opportunity Alert 2/12/08
> To: "'Histonet'" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <E1JOuyP-0004w6-4b <@t> elasmtp-banded.atl.sa.earthlink.net>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hi Histonetters!!
> Just wanted to do a quick post on some of the jobs I am currently
> working on. These clients are all looking for ASCP certified or
> eligible people interested in full time dayshift permanent positions.
> They offer excellent compensation, benefits and relocation
> assistance if
> needed.
> If you or someone you know is interested please contact me and please
> feel free to pass this information along to others as well
> Here is a list of the locations where I have some great opportunities:
> Texas - Multiple locations management and tech positions
> South Carolina - tech position
> Washington - management and tech positions
> Montana - tech position
> Pennsylvania - multiple locations tech positions
> New Hampshire - tech position
> California - tech positions - ASCP certification is required
> Virginia - tech position
>
> Remember it never hurts to look. You can reach me at 866-60-RELIA
> (866-607-3542) or at relia1 <@t> earthlink.net
>
> Have a Happy Valentines Day!!!
>
> Thanks-Pam
>
>
> Thank You!
>
>
> Pam Barker
> President
> RELIA
> Specialists in Allied Healthcare Recruiting
> 5703 Red Bug Lake Road #330
> Winter Springs, FL 32708-4969
> Phone: (407)657-2027
> Cell: (407)353-5070
> Toll Free(866)-607-3542
> Fax: (407)678-2788
> E-mail: relia1 <@t> earthlink.net
> <http://home.earthlink.net/~relia1>
> www.myspace.com/pamatrelia
>
>
>
> ------------------------------
>
> Message: 11
> Date: Tue, 12 Feb 2008 08:11:49 -0600
> From: "Sharon.Davis-Devine" <Sharon.Davis-Devine <@t> carle.com>
> Subject: [Histonet] Formalin recycler
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <44780C571F28624DBB446DE55C4D733A021E08BE <@t> EXCHANGEBE1.carle.com>
> Content-Type: text/plain; charset="us-ascii"
>
> Presently we have two CBG recycling machines; one recycles alcohol and
> the other formalin. We seem to always be backed up on formalin
> because
> it takes so long to run a cycle. Is there some other system out there
> that offers a faster turn around time? Any suggestions would be
> greatly
> appreciated. Thanks.
>
>
>
> Sharon Davis-Devine, CT (ASCP)
>
> Cytology Supervisor
>
> Carle Clinic
>
> 602 West University
>
> Urbana, Illinois 61801
>
> Phone: 217-383-3572
>
> Email: sharon.davis-devine <@t> carle.com
>
>
>
>
>
> ------------------------------
>
> Message: 12
> Date: Tue, 12 Feb 2008 07:27:26 -0800
> From: "Laurie Colbert" <laurie.colbert <@t> huntingtonhospital.com>
> Subject: RE: [Histonet] Formalin recycler
> To: "Sharon.Davis-Devine" <Sharon.Davis-Devine <@t> carle.com>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <57BE698966D5C54EAE8612E8941D76830267DD39 <@t> EXCHANGE3.huntingtonhospital.com
> >
>
> Content-Type: text/plain; charset="us-ascii"
>
> Creative Waste Solutions has a formalin recycler that is very fast and
> easy to use. We love it! We use our CBG recycler for alcohol and
> xylene and the Creative Waste recycler for the formalin. They can be
> reached at (888) 795-8300.
>
> Laurie Colbert
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> Sharon.Davis-Devine
> Sent: Tuesday, February 12, 2008 6:12 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Formalin recycler
>
> Presently we have two CBG recycling machines; one recycles alcohol and
> the other formalin. We seem to always be backed up on formalin
> because
> it takes so long to run a cycle. Is there some other system out there
> that offers a faster turn around time? Any suggestions would be
> greatly
> appreciated. Thanks.
>
>
>
> Sharon Davis-Devine, CT (ASCP)
>
> Cytology Supervisor
>
> Carle Clinic
>
> 602 West University
>
> Urbana, Illinois 61801
>
> Phone: 217-383-3572
>
> Email: sharon.davis-devine <@t> carle.com
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 13
> Date: Tue, 12 Feb 2008 09:59:45 -0600
> From: "Henry, Charlene" <Charlene.Henry <@t> STJUDE.ORG>
> Subject: [Histonet] Opening for a Core Lab Manager
> To: "Histonet <@t> lists.utsouthwestern.edu"
> <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <03E1F5968F60C5448635D49D38B283ED03F507BF <@t> SJMEMXMBS11.stjude.sjcrh.local
> >
>
> Content-Type: text/plain; charset="us-ascii"
>
> We currently have an opening for a Histology Core Lab Manager in our
> new Histology Core Lab that is due to be completed within the next
> couple of months. If you are interested, you can send me an email or
> visit www.stjue.org<http://www.stjue.org>. I'll have to say that St
> Jude is one of the greatest places I have ever worked and would
> recommend this position to anyone looking for a research lab to
> manage.
> Thanks,
>
> Charlene Henry HT (ASCP), QIHC
> Anatomic Pathology Section Head
> Department of Pathology
> St. Jude Children's Research Hospital
> 901-495-3191
> fax 901-495-3100
>
>
>
> ------------------------------
>
> Message: 14
> Date: Tue, 12 Feb 2008 10:29:55 -0600
> From: pkromund <@t> gundluth.org
> Subject: Re: [Histonet] new prostate problem!
> To: Nick and Amanda <nickandmanda <@t> paradise.net.nz>
> Cc: histonet <@t> lists.utsouthwestern.edu,
> histonet-bounces <@t> lists.utsouthwestern.edu
> Message-ID:
> <OFE379128D.0E878F9F-ON862573ED.005A8CA6-862573ED.005AA141 <@t> gundluth.org
> >
>
> Content-Type: text/plain; charset=US-ASCII
>
> Yes, drying prior to being placed in formalin does have adverse
> affects on
> staining.
>
>
>
> Nick and Amanda
> <nickandmanda <@t> par
>
> adise.net.nz> To
> Sent by:
> histonet <@t> lists.utsouthwestern.edu
> histonet-
> bounces@ cc
> lists.utsouthwest
> ern.edu
> Subject
> [Histonet] new prostate problem!
>
> 02/12/2008 01:30
> AM
>
>
>
>
>
>
>
> Hi!
>
> We have recently come up against a staining issue where our prostate
> cores
> have patches which are not staining. Our Pathologist tells us this is
> common in a particular High grade diagnosis so this is a problem
> when it is
> happening in all prostate cases.. It does not seem to be affecting
> other
> tissue staining (perhaps the odd one! no examples could be
> provided!! Could
> it possibly be drying out of the cores prior to being placed in
> formalin??
>
> Any Ideas welcome
>
> Just not sure!
> Wellington, NZ
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 15
> Date: Tue, 12 Feb 2008 10:48:00 -0600
> From: "Henry, Charlene" <Charlene.Henry <@t> STJUDE.ORG>
> Subject: [Histonet] Histology Core Lab Manager Position
> To: "Histonet <@t> lists.utsouthwestern.edu"
> <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <03E1F5968F60C5448635D49D38B283ED03F507C0 <@t> SJMEMXMBS11.stjude.sjcrh.local
> >
>
> Content-Type: text/plain; charset="us-ascii"
>
> In my earlier email, I left out part of the email address and it is
> not even Monday. The correct link is www.stjude.org<http://www.stjude.org
> >; sorry for the confusion.
> Thanks,
>
> Charlene Henry HT (ASCP), QIHC
> Anatomic Pathology Section Head
> Department of Pathology
> St. Jude Children's Research Hospital
> 901-495-3191
> fax 901-495-3100
>
>
>
> ------------------------------
>
> Message: 16
> Date: Tue, 12 Feb 2008 10:59:16 -0600
> From: "Henry, Charlene" <Charlene.Henry <@t> STJUDE.ORG>
> Subject: [Histonet] Histology Core Lab Manager Position
> To: "Histonet <@t> lists.utsouthwestern.edu"
> <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <03E1F5968F60C5448635D49D38B283ED03F507C1 <@t> SJMEMXMBS11.stjude.sjcrh.local
> >
>
> Content-Type: text/plain; charset="us-ascii"
>
> If interested in this position go to www.stjude.org<http://www.stjude.org
> > and under "Jobs" the position # is 17078 and the title of this
> position is "Laboratory Manager Veterinary Histology Core".
> Thanks,
>
> Charlene Henry HT (ASCP), QIHC
> Anatomic Pathology Section Head
> Department of Pathology
> St. Jude Children's Research Hospital
> 901-495-3191
> fax 901-495-3100
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 51, Issue 18
> ****************************************
Laura R Hunt, PhD
Post-doctoral associate
University of Texas at Arlington
ph: 817-272-1499
fax: 817-272-2855
lhunt <@t> uta.edu
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