[Histonet] Histology Grade Solvents

[Smith, Allen]

The "Histology grade" label on solvents is not at all demeaning; it just notes that that bottle of solvent is fit for use in histology.  Histology grade xylene is a mixture of 3 different xylenes and ethylbenzene: it clears tissue beautifully, but the extra absorption peaks would be a real pain in a solvent for spectroscopy, GC, or HPLC. Ethanol denatured with kerosene is a good solvent for organic reactions because kerosene is not very reactive, but its lack of miscibility with water is disastrous in a histology lab.

Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University School of Graduate Medical Sciences
    Podiatric Medicine and Surgery
Miami Shores, Florida  33161


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, February 08, 2008 11:35 AM
To: Mark Elliott; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Histology Grade Solvents

Some manufacturers qualify a solvent that is NOT chemically pure (not good for chemical quantitative analytical purposes) BUT "pure enough" to be used in histology, specially for processing or staining.
  I have always taken exception to this "histology grade" issue because it is somewhat demeaning, as if what we do do not deserve a good quality reagent grade chemical. Those are the standard to prepare our stock staining solutions that should be as pure as possible, especially when silver staining is involved.
  So, "histology grade" reagent is one that we can use, but that a chemist "should not".
  René J.



Mark Elliott <MElliott <@t> mrl.ubc.ca> wrote:
  Good Friday Morning to everyone.-YEAHHH!! It's been one of those weeks:(

I was asked by someone about this and not sure what the answer is.

"I have a quick question for you regarding histology grade solvents. Many
companies use this as a grade but is there an official specification for
this grade? If so, could you direct me to the document."

Any ideas??

Thanks

Mark


***CONFIDENTIALITY NOTICE***
This electronic message is intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system.

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



---------------------------------
Looking for last minute shopping deals?  Find them fast with Yahoo! Search.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet