[Histonet] eosinophils on frozen section

[Julia Dahl]

Agree with Gayle that alcoholic formalin allows for speedier fixation and preservation of eosinophil granule membranes (and therefore staining, rather than degranulation).

> Date: Tue, 5 Feb 2008 15:32:31 -0800
> From: ebreisch <@t> rchsd.org
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] eosinophils on frozen section
> 
> To all interested Histonetters:
> 
>  
> 
>  
> 
> We are experiencing some frustration with our ability to identify
> eosinophils from frozen sections. For some reason which our frozen
> section set up does not enable us to identify eosinophils. Eosinophil
> identification is not impaired when the tissue is submitted for
> permanents. Could some one please share the reagents used and how the
> frozen section staining area is set up if they have success identifying
> eosinophils from a frozen section? We have had many frozen sections done
> on fresh tissues suspicious for an eosinophilic granuloma but our
> techniques so far have not rendered consistent eosinophil
> identification. We presently are using 95% ETOH for fixing the FS slide,
> quick wash in distilled H2O, 1 minute in Richard Allen Hematoxylin 2,
> rinse in tap water, 15 seconds in bluing, rinse in water, rinse in 70%
> ETOH, 1 minute in Richard Allen Eosin Y, three rinses in 95% ETOH, three
> rinses in Xylene substitute and then coverslip. All other tissues appear
> just fine but the eosinophils just don't show up. Any suggestions are
> greatly appreciated.
> 
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> 
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> 
> Eric A. Breisch, Ph.D.
> 
> Clinical Anatomist
> 
> Dept. of Pathology
> 
> Rady Children's Hospital and Health Center
> 
> Associate Clinical Professor of Anatomy
> 
> Dept. of Surgery
> 
> UCSD School of Medicine 
> 
>  
> 
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