[Histonet] sections falling off

[Debrosse-Serra, Beatrice]

Try Newcomers silane coated slides. They are not cheap, but the sections
stay on very well. 

Beatrice DeBrosse-Serra
Pathology Scientist 
Pfizer Global Research & Development
CB4, 2150
10646 Science Center Drive
San Diego, CA 92121
Phone# 858-622-5986
Fax# 858-678-8290



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Olek
Michalski
Sent: Tuesday, February 05, 2008 10:00 AM
To: Histonet
Subject: [Histonet] sections falling off


Dear Histonetters,

a friend of mine just faced a massive sections falling off. She is doing

Nissl staining in mouse brain (brains perfused with formalin, postfixed
3  
days, and cryosectioned) on poly-L-lysined slides. She just managed to  
pass trough xylene and decreasing grades of alcohol to water and the  
sections started to detach. We were trying to reattach them but it went

out that sections are not flat any more. It seems like the tissue is  
rehydrated unevenly, but keeping it in water for hours didn't work. Is  
there any way to spread these sections not damaging them at the same
time?  
She is likely to rescue this sections even if some work is needed.

Best regards
Olek Michalski
-- 
        Laboratory of Neurobiology
       of Development and Evolution

  Nencki Institute of Experimental Biology
  ul. Pasteura 3, 02-093 Warszawa,  Poland
  Tel. +48 22 5892268,  Fax +48 22 8225342


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