[Histonet] bone from cartilage

[Gayle Callis]

Haxel,

Toluidine Blue should do it.   Eurell JA, Sterchi DL. Microwaveable 
toluidine blue stain for surface staining of undecalcified bone sections, J 
Histotechnology, Decembetr 1994. Diane Sterchi developed this stain using pH 
8 phosphate buffer and by heating the staining solution using a microwave. 
Just don't microwave the section while it is in the stain.   It is easy to 
do.   If you are a member of NSH and do not have the journal, you should be 
able to access the publication without fee.  Also, a combination of this T 
blue and MacNeal's tetrachrome.  It will be a more the stains tinctorial 
differences of the articular versus the calcified cartilage that you need to 
get used too. The stain works on both ground, surface stained thick sections 
or microtomed MMA sections.

The Verhoeff's orcein may work but only if you totally remove the MMA from a 
microtomed section using a solvent, hot xylene is one that works.

Gayle M. Callis
HTL/HT/MT(ASCP)
Bozeman MT 59715

----- Original Message ----- 
From: "Rene J Buesa" <rjbuesa <@t> yahoo.com>
To: "Hazel Stevens" <hstevens3 <@t> mail.gatech.edu>; 
<histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, February 04, 2008 8:52 AM
Subject: Re: [Histonet] bone from cartilage


> Use Verhoeff''s orcein.
>  René J.
>
> Hazel Stevens <hstevens3 <@t> mail.gatech.edu> wrote:
>  Hi Histonet ,
>
>
>
> Wondered if anyone could help. I'm looking for a stain that distinguishes
> cartilage (calcified or not) from bone in decalcified growth plate 
> sections
> (MMA). Does such a stain exist?
>
>
>
> Many thanks
>
> Hazel
>
>
>
> Hazel Stevens,
>
> Temporary Research Scientist,
>
> Georgia Tech
>
>
>
>
>
>
>
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