[Histonet] FISH/ISH on Archival slides

[Angela McNabola]

Hi all,

 

I'm a "former" histotech performing FISH on cytology samples (primarily
cervical).  I have been given archival slides, 2-7 years old, that have
been Pap stained.  As you can imagine, they are not necessarily optimal
samples.  Some are even coverslipped with the "tape" method, and it is
taking days to soak it off.

 

I have tried a few variations of destaining, but the problem is that I'm
not getting any FISH signals at all.  Basically we are using a 7
centromere or 11q (commercially obtained).  Does anybody have any
insight, thoughts, or ideas on how to get this to work?  

 

Thanks!

 

Angela

 

 

Angela McNabola, MS HT(ASCP)SLS, QIHC

Research Scientist

Ikonisys, Inc.

5 Science Park

New Haven, CT 06511

203-776-0791

angela.mcnabola <@t> ikonisys.com