From gorhamk <@t> verizon.net Fri Feb 1 06:58:21 2008 From: gorhamk <@t> verizon.net (Kathy Gorham) Date: Fri Feb 1 06:58:32 2008 Subject: [Histonet] scales Message-ID: <000b01c864d2$20749480$2f01a8c0@kathy83b707eca> Good Friday Morning everyone: I have a regulation question: Do we have to calibrate our scales in Histology? And how often? I often check it but never heard we needed a procedure for it until now. Kathy Gorham, H.T. Grande Ronde Hospital LaGrande, Or From Susan.Walzer <@t> HCAHealthcare.com Fri Feb 1 07:02:14 2008 From: Susan.Walzer <@t> HCAHealthcare.com (Walzer Susan) Date: Fri Feb 1 07:02:28 2008 Subject: [Histonet] Round lens paper In-Reply-To: <661949901A768E4F9CC16D8AF8F2838CA1C8A1@IS-E2K3.grhs.net> References: <57BE698966D5C54EAE8612E8941D768302589569@EXCHANGE3.huntingtonhospital.com> <661949901A768E4F9CC16D8AF8F2838CA1C8A1@IS-E2K3.grhs.net> Message-ID: <471953BC63077941B82C26A4338272B42F0572@ORLEV03.hca.corpad.net> We get the round papers from Obex Industries Inc., 2 Greeley Ave., Florence MA 01062 They are called Histo-Wrap and they are the BEST thing for bx's, curetting, etc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Thursday, January 31, 2008 5:36 PM To: Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Round lens paper Try tea bags: http://www.sunburstbottle.com/bags/tea-muslin Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Thursday, January 31, 2008 3:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Round lens paper Does anyone know where I can order round lens paper (not filter paper)? We place these in the little disposable funnels and filter our GI specimens through it. The specimen is then wrapped in this paper and placed in a cassette. Our current vendor is unreliable. Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From valeria.berno <@t> embl.it Fri Feb 1 07:43:19 2008 From: valeria.berno <@t> embl.it (Valeria Berno) Date: Fri Feb 1 07:43:34 2008 Subject: [Histonet] blood smear Message-ID: <1557.10.251.1.146.1201873399.squirrel@10.251.1.146> Hi, As always I will really appreciate if someone could give me some advice... I need to stain peripheral blood with anti-GFP antibody and look with immunofluorescence ("red" seeondary antibody). My question is about the protocol: Is it better blood smear, methanol fixation and antibody staining or is it better to perform staining in the tube and then cytospin? Could you refer me to a protocol? thanks in advance Valeria Berno Valeria, PhD EMBL- Mouse Biology Unit Campus A. Buzzati-Traverso Via Ramarini, 32 00015, Monterotondo Scalo (RM) Italy Tel: +39 06 90091287 Fax: +39 06 90091406 email: valeria.berno@embl.it www.embl.it From rjbuesa <@t> yahoo.com Fri Feb 1 07:43:25 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 1 07:43:39 2008 Subject: [Histonet] Formalin Exposure In-Reply-To: Message-ID: <75560.88137.qm@web61221.mail.yahoo.com> A mixture of ethanol+propanol+mineral oil that totally eliminates xylene with much better infiltration results. Ren? J. Jennifer MacDonald wrote: Just out of curiosity, what clearing agent are you using in the lab? Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Cindy DuBois" Sent by: histonet-bounces@lists.utsouthwestern.edu 01/31/2008 08:56 AM To karenadams@comcast.net, Histonet cc Subject [Histonet] Formalin Exposure I too have an employee with sometimes severe asthma. I told her to tell me when she is having a bad day / week, to let me know and I move her from the grossing area. Yes, I do get some complaints from other employees. But I explained to them I would rather have the one person healthy and able to work than have her off for several days due to her asthma. And if she had a severe attack due to formalin, I believe that would become a workman's comp issue. Cindy DuBois _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From rjbuesa <@t> yahoo.com Fri Feb 1 08:01:52 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 1 08:02:05 2008 Subject: [Histonet] scales In-Reply-To: <000b01c864d2$20749480$2f01a8c0@kathy83b707eca> Message-ID: <375155.18807.qm@web61224.mail.yahoo.com> We had a service that calibrate our scales once a year. We had two old and extremely reliable Mettler scales (mechanical) and they were certified annually. The certification is a requirement if you prepare solutions in the lab and want to be sure of their quality. Ren? J. Kathy Gorham wrote: Good Friday Morning everyone: I have a regulation question: Do we have to calibrate our scales in Histology? And how often? I often check it but never heard we needed a procedure for it until now. Kathy Gorham, H.T. Grande Ronde Hospital LaGrande, Or _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From jennifer.l.hofecker <@t> Vanderbilt.Edu Fri Feb 1 08:12:42 2008 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Fri Feb 1 08:12:55 2008 Subject: [Histonet] scales In-Reply-To: <000b01c864d2$20749480$2f01a8c0@kathy83b707eca> Message-ID: <898D946569A27444B65667A49C074052015643AA@mailbe06.mc.vanderbilt.edu> We have a set of standard weights. We "check" our balance every 6 months. I have a chart that shows exactly what the measurement is in one column (Electric, LCD Mettler balance) and the standard in the other. We mainly do this because with muscle histochemistries, those chemicals can be finicky. Although our last CAP inspector did say the protocol was more than adequate. It must be on the list somewhere, just not sure where at this point. We've never been "off" from the standard weights. I would say at that point you would call service or decipher a "standard deviation" to ensure correct measurements. Good Luck and have a great weekend. Jennifer Jennifer L. Hofecker, HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph. (615)343-0083 fax. (615)343-7089 NSH Quality Control Committee Chair -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy Gorham Sent: Friday, February 01, 2008 6:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] scales Good Friday Morning everyone: I have a regulation question: Do we have to calibrate our scales in Histology? And how often? I often check it but never heard we needed a procedure for it until now. Kathy Gorham, H.T. Grande Ronde Hospital LaGrande, Or _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Fri Feb 1 09:27:10 2008 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Feb 1 09:27:35 2008 Subject: [Histonet] scales In-Reply-To: <000b01c864d2$20749480$2f01a8c0@kathy83b707eca> References: <000b01c864d2$20749480$2f01a8c0@kathy83b707eca> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD320518E4C3@sjhaexc02.sjha.org> It is part of the Gen lab regulations for CAP. We have a company check our annually. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy Gorham Sent: Friday, February 01, 2008 7:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] scales Good Friday Morning everyone: I have a regulation question: Do we have to calibrate our scales in Histology? And how often? I often check it but never heard we needed a procedure for it until now. Kathy Gorham, H.T. Grande Ronde Hospital LaGrande, Or _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From kalschev <@t> svm.vetmed.wisc.edu Fri Feb 1 10:20:04 2008 From: kalschev <@t> svm.vetmed.wisc.edu (Vicki Kalscheur) Date: Fri Feb 1 10:21:40 2008 Subject: [Histonet] X-ray/mouse tissue Message-ID: <006c01c864ee$4dc884c0$c5d76880@vetmed.wisc.edu> Coral, 24 KVP for I minute - top shelf of faxitron. Darker? 24 KVP for 1.5 minutes - top shelf. Film: Kodak LPF 7 works well. Vicki From mpence <@t> grhs.net Fri Feb 1 10:24:42 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Feb 1 10:24:56 2008 Subject: [Histonet] Ventana Users Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C8A4@IS-E2K3.grhs.net> I am going to work up a new antibody. Ventana's Confirm p53. I need to save time. Can any of the Ventana users give me a protocol that works best for you? Thanks in advance, Mike From Teri.Hallada <@t> midmichigan.org Fri Feb 1 10:31:25 2008 From: Teri.Hallada <@t> midmichigan.org (Teri.Hallada@midmichigan.org) Date: Fri Feb 1 10:31:53 2008 Subject: [Histonet] PQRI Message-ID: <8839B08E3ED7364E8CBBD53882C984D50994CBEE@MAILSRV01.midmichigan.net> We are attempting to perform the pathology PQRI on breasts and colons and are having trouble figuring out how to do this on inpatients. Can anyone out there assist us on how they are submitting the codes on inpatients? Teresa Hallada BS, MT/CT (ASCP) Lead Cytotechnologist MidMichigan Health - Gratiot teri.hallada@midmichigan.org 989.463.1101 ext 3423 Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are herby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply. From Jerry <@t> ralambusa.com Fri Feb 1 11:22:35 2008 From: Jerry <@t> ralambusa.com (Jerry Helisek) Date: Fri Feb 1 11:22:53 2008 Subject: [Histonet] RE: Round lens Paper Message-ID: <3855F92002259948A66A8CA2D16E3A4F05A9B6@server.ralambusa.com> Laurie, we may have what you are looking for, see the two links below. see: http://www.ralamb.net/product_info.php?products_id=285 or: http://www.ralamb.net/advanced_search_result.php?keywords=whatman&x=0&y= 0 Have a great day! ------------------------------------ Raymond A. Lamb, Inc Jerry Helisek jerry@ralambusa.com 5409 Lumley Road, Unit 102 Durham, North Carolina 27703 tel: 919.957.1964 fax: 919.957.1972 mobile: 919.264.7964 Skype ID:jerryhelisek ------------------------------------ ------------------------------ Message: 10 Date: Thu, 31 Jan 2008 13:46:38 -0800 From: "Laurie Colbert" Subject: [Histonet] Round lens paper To: Message-ID: <57BE698966D5C54EAE8612E8941D768302589569@EXCHANGE3.huntingtonhospital.c om> Content-Type: text/plain; charset="us-ascii" Does anyone know where I can order round lens paper (not filter paper)? We place these in the little disposable funnels and filter our GI specimens through it. The specimen is then wrapped in this paper and placed in a cassette. Our current vendor is unreliable. Laurie Colbert From a_marcuzzi <@t> cantv.net Fri Feb 1 11:34:57 2008 From: a_marcuzzi <@t> cantv.net (a_marcuzzi@cantv.net) Date: Fri Feb 1 11:35:14 2008 Subject: [Histonet] Tissue Processor Message-ID: <381-22008251173457246@cantv.net> Dear All, Could anyone help me with a copy of a circuit diagram or service manual for a Tissue TekII Processor Model 4640B? Thanks in advance. Regards, Augusto From juditw <@t> u.washington.edu Fri Feb 1 12:37:22 2008 From: juditw <@t> u.washington.edu (Judith L. Williams) Date: Fri Feb 1 12:37:36 2008 Subject: [Histonet] BL2 safety precautions in histo Message-ID: Hi to all histonetters- what are the current standards for BL2 precautions in the la. I am particularly interested in microtomes - for paraffin embedded sectioning of prion infected tissue- do you have to decon the machine after usage? do you have a microtome designated only for that and also decon it? do you have a special room for contaminated specimens? how do you store the blocks? any help/advice greatly appreciated! Judy at U. Washington From michael.owen <@t> fda.hhs.gov Fri Feb 1 12:45:39 2008 From: michael.owen <@t> fda.hhs.gov (Owen, Michael P) Date: Fri Feb 1 12:48:18 2008 Subject: [Histonet] BL2 safety precautions in histo In-Reply-To: Message-ID: <449E51C6DA0AD840B44F57C7A6EB07BF042203CB@FMD3VS022.fda.gov> Dear Judy, The American Biological Safety Association (ABSA) and its Biosafety Mailing List are two great resources to ask your questions. American Biological Safety Association (ABSA) http://www.absa.org ABSA: E-Mail Groups http://www.absa.org/resgroups.html Sincerely, Michael Michael P. Owen, Regulatory Microbiologist U.S. FDA Pacific Regional Lab Northwest 22201 23rd Drive SE Bothell, WA 98021-4421 Phone: 425-483-4865 E-Mail: michael.owen@fda.hhs.gov From Warren_Eddings <@t> ssmhc.com Fri Feb 1 12:52:01 2008 From: Warren_Eddings <@t> ssmhc.com (Warren_Eddings@ssmhc.com) Date: Fri Feb 1 12:52:37 2008 Subject: [Histonet] Re: Histonet Digest, Vol 51, Issue 2 Message-ID: does anyone validate antibody and how do you _________________________________________________________________ Con attachments, is for may contain confidential and unauthorized review, use, disclosure or distr prohibited. If you are not the intended recipient, please cont the sender by reply email and destroy all copies of the original messag From shive003 <@t> umn.edu Fri Feb 1 12:54:25 2008 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Feb 1 12:54:40 2008 Subject: [Histonet] BL2 safety precautions in histo References: Message-ID: <010901c86503$ddac8f40$b0065486@auxs.umn.edu> Yes to the first three questions; blocks are stored in the same room as the designated microtome. We work on Chronic Wasting Disease and Scrapie, both abnormal prion protein diseases. Jan Shivers Senior Scientist Histology/IHC/EM Section Head University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003@umn.edu ----- Original Message ----- From: "Judith L. Williams" To: Sent: Friday, February 01, 2008 12:37 PM Subject: [Histonet] BL2 safety precautions in histo > Hi to all histonetters- what are the current standards for BL2 > precautions in the la. I am particularly interested in microtomes - for > paraffin embedded sectioning of prion infected tissue- > do you have to decon the machine after usage? > do you have a microtome designated only for that and also decon it? > do you have a special room for contaminated specimens? > how do you store the blocks? > > any help/advice greatly appreciated! > > Judy at U. Washington > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From hej01 <@t> health.state.ny.us Fri Feb 1 13:05:36 2008 From: hej01 <@t> health.state.ny.us (Helen E Johnson) Date: Fri Feb 1 13:21:44 2008 Subject: [Histonet] paraffin block dewaxer Message-ID: Hi Histonetters, Which paraffin block dewaxer would you recommend? Helen Johnson (hej01@health.state.ny.us) IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. From detmar <@t> mshri.on.ca Fri Feb 1 13:24:40 2008 From: detmar <@t> mshri.on.ca (Jacqui Detmar) Date: Fri Feb 1 13:24:59 2008 Subject: [Histonet] need advice on labelling primary antibody with fluorophore; also need suggestions for good NF-kB antibody against mouse tissue Message-ID: Hi all. Hope you are having a nice afternoon. I live in Toronto, so I'm trying to avoid looking out the window at all that snow coming down . Anyway, I have a couple of questions to ask those in histo-land: 1. I am looking for a kit and/or advice that will help me to label a green fluorophore (possibly fluorescein?) to a primary antibody, that also comes with it's own anti-"green fluorophore" secondary antibody (also labeled with a green fluorophore). I am doing double-labelling studies using two different primaries from the same host species. In the future I will also likely be doing mouse-on-mouse staining and hope to circumvent high non-specific staining by labeling with a fluorophore. 2. Does anybody have any experience with a good NF-kB antibody for IHC on FFPE mouse tissues? I would love to know what works for you. Thanks all, and enjoy the weekend, Jacqui Jacqui Detmar, Post-doctoral Fellow Samuel Lunenfeld Research Institute, room 876 Mount Sinai Hospital 600 University Avenue Toronto, ON, Canada M5G 1X5 phone: 416-586-4800 x2451/x2290 fax: 416-586-8588 email: detmar@mshri.on.ca From liz <@t> premierlab.com Fri Feb 1 13:36:46 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Feb 1 13:36:57 2008 Subject: [Histonet] paraffin block dewaxer In-Reply-To: References: Message-ID: We have the thermo one in the lab, but it depends upon the tech, some use it and some don't, I personally don't use it I use a knife to scrap the blocks, if there are lots a blocks I might use it, but it works well. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Helen E Johnson Sent: Friday, February 01, 2008 12:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] paraffin block dewaxer Hi Histonetters, Which paraffin block dewaxer would you recommend? Helen Johnson (hej01@health.state.ny.us) IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ploykasek <@t> phenopath.com Fri Feb 1 13:53:35 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri Feb 1 13:53:56 2008 Subject: [Histonet] Ab validation In-Reply-To: Message-ID: ------ Forwarded Message From: Patti Loykasek Date: Fri, 01 Feb 2008 11:18:26 -0800 To: Subject: Re: [Histonet] Re: Histonet Digest, Vol 51, Issue 2 I'll try to formulate a brief answer to the question of validating antibodies. If you are doing IHC both CLIA and CAP have regulations that involve validation of antibodies. These regulations cover Establishment and Verification of Method Performance Specifications. The validation process is designed to confirm the ability of the antibody to recognize the target antigen in normal and diseased tissues where it is reasonably expected to localize. And that there is not any obvious unexpected expression; in other words, to determine its specificity and sensitivity. To validate antibodies for IHC it's a multiple step process. First you would need to determine the working titer, pretreatment, detection & chromogen that you are going to use. Use your standard SOPS. Use a control that contains the antigen in question plus some negative elements. You don't want a control that is a high expressor or too low of an expresser at this point. Once you?ve determined your working parameters, you need to test the antibody on normal tissue & diseased tissue that does & does not contain the antigen. For example, if you are validating an antibody that is positive in Lung carcinomas you would want to use lung carcinomas (should be positive), & a variety of carcinomas, lymphomas, etc... That should be negative. You may find that this antibody is positive in GI carcinoma, too & you would want to document that & know what % of GI carcinomas are positive, Also, run some normal tissues & assess their positive & negative rate. W compare our findings with the published literature. You need to document all this work ( if you don?t document it, it didn?t happen). Excel or some other software is good for documenting your findings. We have a template that we use for all work ups (& a validation SOP). Hope this helps. Good luck. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > > does anyone validate antibody and how do you = do it? > _________________________________________________________________ > > > > > > > > Con= fidentiality Notice: This email message, including any > attachments, is for = the sole use of the intended recipient(s) and > may contain confidential and = privileged information. Any > unauthorized review, use, disclosure or distr= ibution is > prohibited. If you are not the intended recipient, please cont= act > the sender by reply email and destroy all copies of the original > messag= e. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------ End of Forwarded Message This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From gayle.callis <@t> bresnan.net Fri Feb 1 15:15:18 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Fri Feb 1 15:15:33 2008 Subject: [Histonet] BL2 safety precautions in histo References: <449E51C6DA0AD840B44F57C7A6EB07BF042203CB@FMD3VS022.fda.gov> Message-ID: <000a01c86517$8c456370$6601a8c0@Sunney> CDC also has guidelines, and a similar question was asked in the past few weeks Histonet. Check Histonet Archives for more on the subject. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59717 ----- Original Message ----- From: "Owen, Michael P" To: "Histonet" Sent: Friday, February 01, 2008 11:45 AM Subject: RE: [Histonet] BL2 safety precautions in histo Dear Judy, The American Biological Safety Association (ABSA) and its Biosafety Mailing List are two great resources to ask your questions. American Biological Safety Association (ABSA) http://www.absa.org ABSA: E-Mail Groups http://www.absa.org/resgroups.html Sincerely, Michael Michael P. Owen, Regulatory Microbiologist U.S. FDA Pacific Regional Lab Northwest 22201 23rd Drive SE Bothell, WA 98021-4421 Phone: 425-483-4865 E-Mail: michael.owen@fda.hhs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From judi.ford <@t> roche.com Fri Feb 1 15:17:32 2008 From: judi.ford <@t> roche.com (Ford, Judi) Date: Fri Feb 1 15:17:49 2008 Subject: [Histonet] Liver samples Message-ID: Hi everyone, One of my coworkers is looking for a rat liver sample with hepatocyte phospholipid accumulation. If anyone knows where she can find such a sample I will pass that information on to her. Thanks sooooo much! Judi Ford, BA, HTL(ASCP) Research Associate III Roche Palo Alto 3431 Hillview Ave Palo Alto, CA 94304 650+855-6122 From rosenfeldtek <@t> hotmail.com Fri Feb 1 15:34:23 2008 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Fri Feb 1 15:34:39 2008 Subject: [Histonet] Alcian Blue Quality Control Message-ID: I have used Alcian Blue 8GX from Sigma for years with pretty good results. But I have had some variations in staining lately and noticed that the powder in the new lot isn't quite as dark as the previous lot. Finally, I noticed that Sigma only guarantees the product to be have a dye content of "at least" 50%. I would like to try to assay the dye content myself, so I could make my solutions to the sane strength every time. Sigma says they don't know hw, they have no idea what the molar extinction coefficient of the compound is, and they refuse to tell me the name of the lab that does the QC. The only thing I know is that it has a major absorbance peak at 610 nm. Oh, rumor says there was an aticle about it in Histochemie back in 1972. Any ideas? Jerry Ricks Research Scientist University of Washington Department of Pathology _________________________________________________________________ Need to know the score, the latest news, or you need your Hotmail?-get your "fix". http://www.msnmobilefix.com/Default.aspx From gayle.callis <@t> bresnan.net Fri Feb 1 15:39:20 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Fri Feb 1 15:39:44 2008 Subject: [Histonet] need advice on labelling primary antibody withfluorophore; also need suggestions for good NF-kB antibody against mouse tissue References: Message-ID: <001d01c8651a$e797cee0$6601a8c0@Sunney> Molecular Probes/Invitrogen has kits to label antibodies with Alexa 488 or other Alexa fluorophores. These are excellent, brighter than FITC and rhodamines, and more resistant to fading. We do double labellling, even triple immunofluorescent staining with antibodies from the same species (all rat antiMouse) using two biotinylated antibodies with two different Alexa fluor labeled Strepavidins, and one purified primary antibody followed by a secondary fluorophore conjugate. For some cells, doing direct IFA on tissue sections doesn't work as the fluorophore quenches itself, so it will not fluoresce. The antibody will bind the problem is the fluorophore. Quenching is NOT fading or photobleaching but occurs when there are i.e. FITC molecules too close to each other. Then quenching occurs. I am not sure if this happens with Alexa dyes, but it may. We cannot see CD4 cells detected with a rat antiMouse CD4-FITC conjugate, and if we use this primary, we have to detect with an antiFITC antibody. Quenching may not occur with all antibody-fluorophore, but we have occur several CD markers. With double and/or triple IFA staining, we do special blocking to ensure there is no non specific binding of secondaries to primaries, etc. Mouse on mouse staining will be a bit different, but one should be able to work with biotinylated primaries and Strepavidin Alexa dyes plus Strepavidin/biotin blocking steps. I will be happy to discuss this with you privately, if you wish. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Jacqui Detmar" To: Sent: Friday, February 01, 2008 12:24 PM Subject: [Histonet] need advice on labelling primary antibody withfluorophore;also need suggestions for good NF-kB antibody against mouse tissue Hi all. Hope you are having a nice afternoon. I live in Toronto, so I'm trying to avoid looking out the window at all that snow coming down . Anyway, I have a couple of questions to ask those in histo-land: 1. I am looking for a kit and/or advice that will help me to label a green fluorophore (possibly fluorescein?) to a primary antibody, that also comes with it's own anti-"green fluorophore" secondary antibody (also labeled with a green fluorophore). I am doing double-labelling studies using two different primaries from the same host species. In the future I will also likely be doing mouse-on-mouse staining and hope to circumvent high non-specific staining by labeling with a fluorophore. 2. Does anybody have any experience with a good NF-kB antibody for IHC on FFPE mouse tissues? I would love to know what works for you. Thanks all, and enjoy the weekend, Jacqui Jacqui Detmar, Post-doctoral Fellow Samuel Lunenfeld Research Institute, room 876 Mount Sinai Hospital 600 University Avenue Toronto, ON, Canada M5G 1X5 phone: 416-586-4800 x2451/x2290 fax: 416-586-8588 email: detmar@mshri.on.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Fri Feb 1 16:09:33 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Fri Feb 1 16:09:48 2008 Subject: [Histonet] Murine CD4, CD8, CD3, Dendritic cell, neutrophil and macrophage monoclonal antibodies touted to work on paraffin embedded tissues References: Message-ID: <003d01c8651f$201dbaf0$6601a8c0@Sunney> Dear Histonetters always looking for these murine CD markers that work on paraffin embedded mouse issues. I was sent the following information, and you can use the links to see what this company has to offer that may make ones life easier instead of cryosections. The fixative was NOT formalin but Bouins. My researcher is very interested. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 597175 Angio-Proteomie rat anti-mouse dendritic cell marker which works in FC and IHC (Paraffin). For detailed click (anti mouse Dendritic cell marker): http://www.angioproteomie.com/Normal%20Mouse%20ab/mAP-0042%20rat%20anti%20mouse%20dendritic%20cells.pdf Now Angio-Proteomie is providing the following monoclonal antibodies for Immunology/Inflammation which can be used for IHC (Paraffin), as well as flow cytometery for mouse leukocytes: for more information click the following links anti mouse CD3 http://www.angioproteomie.com/Normal%20Mouse%20ab/mAP-0027%20rat%20anti%20mouse%20T-Cell.pdf anti mouse CD4 http://www.angioproteomie.com/Normal%20Mouse%20ab/mAP-0035%20rat%20anti%20mouse%20CD4.pdf anti mouse CD8a http://www.angioproteomie.com/Normal%20Mouse%20ab/mAP-0036%20rat%20anti%20mouse%20CD8a.pdf anti mouse Neutrophils http://www.angioproteomie.com/Normal%20Mouse%20ab/mAP-0028%20rat%20anti%20mouse%20Neutrophil.pdf anti mouse Macrophages http://www.angioproteomie.com/Normal%20Mouse%20ab/mAP-0028%20rat%20anti%20mouse%20Neutrophil.pdf Helen S Yang, PhD Angio-Proteomie 11 Park Drive, Suite 12 MA 02215 Tel: 617-549-2665 Fax: 480-247-4337 From gmartin <@t> marshallmedical.org Fri Feb 1 16:11:26 2008 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Fri Feb 1 16:11:38 2008 Subject: [Histonet] HP staining Message-ID: <6ED9D4252F278841A0593D3D788AF24C01A85F72@mailsvr.MARSHMED.local> My pathologist is asking me to find a rapid stain for helicobacter pylori that stains the mucin yellow. Thanks in advance Gary From akemiat3377 <@t> yahoo.com Fri Feb 1 16:55:46 2008 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Fri Feb 1 16:55:58 2008 Subject: [Histonet] Alcian Blue Quality Control In-Reply-To: Message-ID: <749102.84213.qm@web31308.mail.mud.yahoo.com> Hi JR R, You can go on line to the Biological Stains Commission website and check out who else supplies Alcian Blue. It won't give you the % of dye content, but will tell you the various companies that have passed their testing parameters. I for one like Anatechs' Alcian Blue dye. They have always had a very consistant dye. Hope this helps Akemi --- JR R wrote: > > I have used Alcian Blue 8GX from Sigma for years > with pretty good results. But I have had some > variations in staining lately and noticed that the > powder in the new lot isn't quite as dark as the > previous lot. Finally, I noticed that Sigma only > guarantees the product to be have a dye content of > "at least" 50%. > > > I would like to try to assay the dye content myself, > so I could make my solutions to the sane strength > every time. Sigma says they don't know hw, they > have no idea what the molar extinction coefficient > of the compound is, and they refuse to tell me the > name of the lab that does the QC. > > > The only thing I know is that it has a major > absorbance peak at 610 nm. > > Oh, rumor says there was an aticle about it in > Histochemie back in 1972. > > Any ideas? > > Jerry Ricks > Research Scientist > University of Washington > Department of Pathology > > > _________________________________________________________________ > Need to know the score, the latest news, or you need > your Hotmail?-get your "fix". > http://www.msnmobilefix.com/Default.aspx_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Akemi Allison-Tacha, BS, HT(ASCP)HTL Client Services Manager PhenoPath laboratories 551 North 34th Street, Suite 100 Seattle, WA 98103-8675 Work: (206) 374-9000 Cell: (425) 941-4287 E-Mail: akemiat3377@yahoo.com From rjbuesa <@t> yahoo.com Fri Feb 1 16:57:27 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 1 16:57:37 2008 Subject: [Histonet] Alcian Blue Quality Control In-Reply-To: Message-ID: <26431.69226.qm@web61211.mail.yahoo.com> You cannot go much with the wave length absorption, because that depends on the color (molecular struicture) and not with the contents. You would have to have a calibration curve to determine absorption coefficient versus concentration, and that is where you get again to your concentration incognita. Do you still have some amount of your original dye? If you do, weight a small amount. Weight the same amount of your actual powder and dissolve both amounts in equal and small amounts of distilled water. Then compare their extintion coefficients at the the same wave length. You will not be able to quantify either concentration but you will find out which is stronger. You could even know how much stronger one is to respect (as a ratio between both extinsion coefficients) to the other and adjust your amounts for your working solutions accordingly. In that way you can assure equally intense staining solutions always, even if you do not know the dye contents in neither. All chemical companies are very tight-lip about their "proprietary" products. Ren? J. JR R wrote: I have used Alcian Blue 8GX from Sigma for years with pretty good results. But I have had some variations in staining lately and noticed that the powder in the new lot isn't quite as dark as the previous lot. Finally, I noticed that Sigma only guarantees the product to be have a dye content of "at least" 50%. I would like to try to assay the dye content myself, so I could make my solutions to the sane strength every time. Sigma says they don't know hw, they have no idea what the molar extinction coefficient of the compound is, and they refuse to tell me the name of the lab that does the QC. The only thing I know is that it has a major absorbance peak at 610 nm. Oh, rumor says there was an aticle about it in Histochemie back in 1972. Any ideas? Jerry Ricks Research Scientist University of Washington Department of Pathology _________________________________________________________________ Need to know the score, the latest news, or you need your Hotmail?-get your "fix". http://www.msnmobilefix.com/Default.aspx_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From rjbuesa <@t> yahoo.com Fri Feb 1 16:59:02 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 1 16:59:13 2008 Subject: [Histonet] HP staining In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C01A85F72@mailsvr.MARSHMED.local> Message-ID: <282605.83696.qm@web61216.mail.yahoo.com> What is he concerned about: the bacteria or the mucin? I find it a rather bizarre request. Ren? J. "Martin, Gary" wrote: My pathologist is asking me to find a rapid stain for helicobacter pylori that stains the mucin yellow. Thanks in advance Gary _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From JWEEMS <@t> sjha.org Fri Feb 1 17:06:37 2008 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Feb 1 17:06:55 2008 Subject: [Histonet] HP staining In-Reply-To: <282605.83696.qm@web61216.mail.yahoo.com> References: <6ED9D4252F278841A0593D3D788AF24C01A85F72@mailsvr.MARSHMED.local> <282605.83696.qm@web61216.mail.yahoo.com> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD320518E4ED@sjhaexc02.sjha.org> It's Alcian yellow - toluidine blue - Here's something about it from Anatech... http://www.anatechltdusa.com/Innovators/7_InnHp.html Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From JWEEMS <@t> sjha.org Fri Feb 1 17:20:14 2008 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Feb 1 17:20:28 2008 Subject: [Histonet] HP staining In-Reply-To: <282605.83696.qm@web61216.mail.yahoo.com> References: <6ED9D4252F278841A0593D3D788AF24C01A85F72@mailsvr.MARSHMED.local> <282605.83696.qm@web61216.mail.yahoo.com> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD320518E4EE@sjhaexc02.sjha.org> Oops - pressed send too soon... This was from Dick Dapson back in 2001. I had gotten some Alcian Yellow from Sigma - stain was beautiful, but our pathologists preferred silver.. We do the Genta. To all of you who use Alcian yellow: Alcian yellow is no longer made, and probably will not be made again because the starting material for its synthesis is not available either. We have been working hard to find an alternative that is indistinguishable from Alcian yellow, and are please to report that we have some promising leads. We need another 6 months or so to conduct shelf-life studies. In the meantime, you can conserve your dye (if you make up your own solutions) by using only 0.2% instead of the usually prescribed 1.0 %. The dye simply is not very soluble in water, and a 0.2% solution (0.2 g per 100 ml) actually has about as much dissolved dye as one made with 1.0 g per 100 ml. Good luck! j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, February 01, 2008 5:59 PM To: Martin, Gary; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HP staining What is he concerned about: the bacteria or the mucin? I find it a rather bizarre request. Ren? J. "Martin, Gary" wrote: My pathologist is asking me to find a rapid stain for helicobacter pylori that stains the mucin yellow. Thanks in advance Gary _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From billions1998 <@t> hotmail.com Fri Feb 1 19:09:54 2008 From: billions1998 <@t> hotmail.com (Sinoera Tech) Date: Fri Feb 1 19:42:19 2008 Subject: [Histonet] Alcian Yellow Alcian Blue In-Reply-To: <001d01c8651a$e797cee0$6601a8c0@Sunney> References: <001d01c8651a$e797cee0$6601a8c0@Sunney> Message-ID: Dear Sirs, We manufacture Alcian Yellow and Alcian Blue. Kind Regards. - Minggeng Wang, Ph.D / President SUZHOU SINOERA CHEM CO., LTD. 125 Binhe Road Suzhou New & Hi-Tech District 215011 China Fax: +86 512 68258994 Tel: +86 512 68246939 http://www.sinoeratech.com From histo227 <@t> verizon.net Sat Feb 2 11:32:24 2008 From: histo227 <@t> verizon.net (Lynne Bell) Date: Sat Feb 2 11:26:28 2008 Subject: [Histonet] HP staining References: <6ED9D4252F278841A0593D3D788AF24C01A85F72@mailsvr.MARSHMED.local> Message-ID: <002d01c865c1$93804650$6b01a8c0@gibson> We have been using the kit from Newcomer Supply - Alcian yellow and Toluidine blue. Our pathologists love it and it stains the mucin yellow. Much easier to find the "bugs". I am at home and don't have the phone number or catalog number with me, but I'm sure you can Google it. Lynne Bell, HT (ASCP) Central VT Medical Center 130 Fisher Road Barre, VT 05641 802-371-4923 From igor.deyneko <@t> gmail.com Sat Feb 2 13:57:11 2008 From: igor.deyneko <@t> gmail.com (Igor Deyneko) Date: Sat Feb 2 13:57:22 2008 Subject: [Histonet] Indian Hedgehog Ab Message-ID: <35e16a770802021157t7d46b7f8r9252b0a4fe23ff8c@mail.gmail.com> Dear Histonetters! I was wondering if anyone has ever happened to work with anti-Human Indian Hedgehog either a Rabbit mono- or polyclonal Ab??? We purchased one from Abcam, but are not satisfied with IHC results. We get staining all over the place: smooth muscle(which is not uncommon), but also vessels, tumor cells, interstitial space, and fibrotic tissue. We get similar results in a positive control, even though according to Abcam claims that should not be happening. Since we are working with xenografts we have human tumor cells with mouse fibrotic tissue support. I tried various rodent and mouse blocks and snipers, with no success. That's why I'm wondering if anyone can advise me an Ab, in which they would be confident. Thank you very much in advance. Igor Deyneko In-Vivo Pharmacology Infinity Pharmaceuticals Cambridge, MA From carl.hobbs <@t> kcl.ac.uk Sat Feb 2 15:00:46 2008 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Sat Feb 2 15:01:07 2008 Subject: [Histonet] re: good NF-kB antibody Message-ID: <003e01c865de$aed3a470$4001a8c0@carlba65530bda> I am currently testing out a SCruz mouse anti NFkB Ab....... I tested it on a mouse transgenic over-expressor of NFKB.....nothing, NUCLEAR! However, in the trans. model there was a lot of lymphocytic infilrate that was +++ for this Ab, cytoplasmic......sure , there was a plasma cell positivity that I subsequently realised was due to MOM. NOTHING Nuclear, sigh, Sure, I used a wt mouse spleen as a control. A difficult new area for pwax sections Carlos From RSRICHMOND <@t> aol.com Sat Feb 2 19:17:17 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Sat Feb 2 19:17:30 2008 Subject: [Histonet] Re: Alcian Blue Quality Control Message-ID: I've posted variations on this comment so many times that I'm sure that many of you think I work for Anatech, but I have no connection with them at all. The Alcian dyes are textile dyes that were made in Germany by the spiritual heirs of I.G. Farbenindustrie of Hitler time renown. Germany finally concluded that there was no way to manufacture these dyes without unacceptable environmental hazards, and banned their manufacture. Alcian dyes have long since passed out of use in the textile industry, but the methods for synthesizing these dyes have to my knowledge never been published. Alcian dyes are now made in India and China, using methods that have never been disclosed. Meanwhile Dick Dapson at Anatech devised synthetic pathways that were safe for chemical workers and for the environment. His Alcian blue was the equivalent of the original dye. Anatech sells Alcian blue to a number of other suppliers. If I were responsible for purchasing, I would insist on this environmentally safe product, but when your purchasing decisions are made by high school graduates backed by bean counters, you have no such authority. The story with Alcian yellow was somewhat different. Dick Dapson stated (I hope I'm quoting correctly) that he could make Alcian yellow, but could not make a product with adequate shelf life. Anatech thereafter introduced a blue and yellow technique for staining Helicobacter, which I have not had the opportunity to work with, though everything I've heard about it has been good. In the personal opinion of this grumpy old pathologist - if I have 20 cases to sign out today, with one gastric biopsy, I'm quite happy to have a toluidine blue or Giemsa or Diff-Quik II stain, and spend a few minutes going over it with an oil immersion lens. If I have to sign out 70 cases with 10 gastric biopsies, I want an immunostain for H.p. I've lately replaced most of the old tungsten-filament light bulbs in my house with energy-efficient compact fluorescent lights (CFL's). All of these bulbs are made in China - I've checked many brands in many stores - and each of them contains about 5 mg of mercury. I wish I had some assurance that the workers who make these bulbs are protected from exposure to mercury. It's time to think about these things, folks, it's all one world, and workers everywhere deserve the same protections in their workplace. Bob Richmond Samurai Pathologist Knoxville TN From WWmn916 <@t> aol.com Sat Feb 2 19:45:55 2008 From: WWmn916 <@t> aol.com (WWmn916@aol.com) Date: Sat Feb 2 19:46:21 2008 Subject: Fwd: [Histonet] Silica water testing Message-ID: Hello everyone, I have three questions about silica testing for Type II water: 1) NCCLS guidelines state max silica content at 0.1 mg/L and that silicates may interfere with certain assays. Assay's like IPOX staining and why? IPOX staining? 2) Can water from same source be tested as Type II for microbial content and tested as Type III for silica? 3) What do others do about silica levels that are above NCCLS guidelines? Thanks in advance Deb King, HT Sacramento, CA **************Biggest Grammy Award surprises of all time on AOL Music. (http://music.aol.com/grammys/pictures/never-won-a-grammy?NCID=aolcmp003000000025 48) From kpilarc <@t> comcast.net Sun Feb 3 04:51:26 2008 From: kpilarc <@t> comcast.net (karen p) Date: Sun Feb 3 07:53:13 2008 Subject: [Histonet] mercurochrome for biopsy marking Message-ID: There is a request for mercurochrome solution for marking our GI biopsies. I'm searching the web for a recipe ffor its prep (I know it involves alcohol and that we filter it in the end, but don't know what amounts, etc). We have a bottle of the crystals. Does someone have an older book that might have the recipe in it? If yes, can you email it to me? I've been searching with little luck, and it would be much appreciated. If we can absolutely cannot find this, then we might consider alternatives-- but not yet. :) Thanks. -karen From rjbuesa <@t> yahoo.com Sun Feb 3 09:39:16 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Feb 3 09:39:28 2008 Subject: [Histonet] Re: Alcian Blue Quality Control In-Reply-To: Message-ID: <576149.58390.qm@web61224.mail.yahoo.com> Hi Samurai: I have forwarded your concerns about the Chinese workers' wellbeing to the "deputy in charge of mercury contamination prevention" in the Central Committee of the Chinese Communist Party of the People's Republic of China. No answers yet, though! Ren? J. Robert Richmond wrote: --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From rjbuesa <@t> yahoo.com Sun Feb 3 09:59:43 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Feb 3 09:59:56 2008 Subject: [Histonet] mercurochrome for biopsy marking Message-ID: <249037.80220.qm@web61215.mail.yahoo.com> Karen: I also have one of those old bottles of di-brom-oxy-mercuri-fluorescein (mercurochrome 220). Just prepare a 2% aquaeous solution and it will do (no ethanol involved). Great stuf for skin cuts and bruises! You could also add a few drops of concentrated eosin in the last absolute ethanol station in your tissue processor and your small biopsies will be stained. Since the next step will probably be xylene (or a substitute) the biopsies will not be distained and will be visible when embedding. Ren? J. karen p wrote: --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From gayle.callis <@t> bresnan.net Sun Feb 3 11:09:38 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Sun Feb 3 11:09:50 2008 Subject: [Histonet] Calculating amount of dye needed from new dye lot Re: Alcian Blue Quality control Message-ID: <005401c86687$8f0e3430$6601a8c0@Sunney> It seemed to me that having to reanalyze a dye for its content was complicated and time consuming. As I cruised through past J of Histotechnology issues, I ran across a simple formula that may help speed things up. X gram needed from new dye lot = 1 gram x % of dye in old dye lot % dye content of new dye We used to do this for the pyronin Y dye in a Methyl green pyronin method for DNA when dye lots were inconsistent and highly variable. Good luck Gayle M. Callis HTL/TH/MT(ASCP) Bozeman MT 59715 From gayle.callis <@t> bresnan.net Sun Feb 3 11:34:10 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Sun Feb 3 11:34:25 2008 Subject: [Histonet] mercurochrome for biopsy marking References: <249037.80220.qm@web61215.mail.yahoo.com> Message-ID: <006501c8668a$fc5b21d0$6601a8c0@Sunney> Histonet has addressed mecurochrome in the past, and isn't it recommended that its use be discontinued because of mercury content in the solution? If so, then Rene's (and many others) suggestion on using eosin is a safer dye for biopsies. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Rene J Buesa" To: "karen p" ; Sent: Sunday, February 03, 2008 8:59 AM Subject: Re: [Histonet] mercurochrome for biopsy marking > Karen: > I also have one of those old bottles of di-brom-oxy-mercuri-fluorescein > (mercurochrome 220). Just prepare a 2% aquaeous solution and it will do > (no ethanol involved). Great stuf for skin cuts and bruises! > You could also add a few drops of concentrated eosin in the last absolute > ethanol station in your tissue processor and your small biopsies will be > stained. Since the next step will probably be xylene (or a substitute) the > biopsies will not be distained and will be visible when embedding. > Ren? J. > > karen p wrote: > > > > > > > --------------------------------- > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it > now. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Sun Feb 3 13:10:20 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Sun Feb 3 13:10:33 2008 Subject: [Histonet] Re: mercurochrome for biopsy marking Message-ID: Indeed, we've discussed the use of Mercurochrome (merbromin) for marking small biopsy specimens several times. Merbromin contains an enormous amount of mercury, and using it will contaminate your system with mercury - same issue as B-5 fixative. The Food and Drug Administration (FDA) banned Mercurochrome as a patent medicine several years ago, and merbromin is no longer available in this form in the USA (or, I think, anywhere). You can mark specimens with eosin. One of my locum tenens clients - as I recently posted - uses the safranin solution used in the conventional Gram stain (as done on smears, not tissue sections) for marking small specimens, and I have been very much pleased with the results - much better recovery of very small specimens by the embedder. As all of our eyes age, this is really worth doing. Bob Richmond Samurai Pathologist Knoxville TN From AnthonyH <@t> chw.edu.au Sun Feb 3 16:45:44 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Feb 3 16:46:25 2008 Subject: [Histonet] mercurochrome for biopsy marking Message-ID: Has anyone noticed that mercurochrome contains MERCURY? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Histopathology The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, 4 February 2008 3:00 AM To: karen p; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] mercurochrome for biopsy marking Karen: I also have one of those old bottles of di-brom-oxy-mercuri-fluorescein (mercurochrome 220). Just prepare a 2% aquaeous solution and it will do (no ethanol involved). Great stuf for skin cuts and bruises! You could also add a few drops of concentrated eosin in the last absolute ethanol station in your tissue processor and your small biopsies will be stained. Since the next step will probably be xylene (or a substitute) the biopsies will not be distained and will be visible when embedding. Ren? J. karen p wrote: --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From tissuearray <@t> hotmail.com Sun Feb 3 20:11:00 2008 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Sun Feb 3 20:11:17 2008 Subject: [Histonet] Tissue Micro Arrays Message-ID: Hey all, I am trying to compile proven methods Array Technicians have use tissue markers to help locate the first position of an array. I have used tonsil and other control tissues for location markers. But I mostly uses died lung tissues. This is how we distinguish one group of 100 cores from another. Each group has a different color location marker. This is what our doctor's have preferred. Any one else want to share other ideas or ways they have made location markers for TMAs . Thanks a bunch, Thom Histologist/TMA Technician For Free TMA instruction go to: www.arrayworkshop.com For New TMA Instrument go to: www.arraymold.com _________________________________________________________________ Helping your favorite cause is as easy as instant messaging.?You IM, we give. http://im.live.com/Messenger/IM/Home/?source=text_hotmail_join From billions1998 <@t> hotmail.com Sun Feb 3 22:13:11 2008 From: billions1998 <@t> hotmail.com (Sinoera Tech) Date: Sun Feb 3 22:13:52 2008 Subject: [Histonet] Re: Alcian Blue Quality Control In-Reply-To: <576149.58390.qm@web61224.mail.yahoo.com> References: <576149.58390.qm@web61224.mail.yahoo.com> Message-ID: Dear Sirs, No mercury will be used in the manufacturing of Alcian Blue! If you are a chemist, this is a normal knowledge. Maybe in USA some manufactures are using mercury to manufacture this dye. It's so funny. Kind Regards. - Minggeng Wang, Ph.D / President SUZHOU SINOERA CHEM CO., LTD. 125 Binhe Road Suzhou New & Hi-Tech District 215011 China Fax: +86 512 68258994 Tel: +86 512 68246939 http://www.sinoeratech.com From: Rene J Buesa Sent: Sunday, February 03, 2008 11:39 PM To: Robert Richmond ; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Alcian Blue Quality Control Hi Samurai: I have forwarded your concerns about the Chinese workers' wellbeing to the "deputy in charge of mercury contamination prevention" in the Central Committee of the Chinese Communist Party of the People's Republic of China. No answers yet, though! Ren? J. Robert Richmond wrote: --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From moran.elish <@t> gmail.com Mon Feb 4 03:24:58 2008 From: moran.elish <@t> gmail.com (Moran Elishmereni) Date: Mon Feb 4 03:25:10 2008 Subject: [Histonet] I need a protocol for congo red/tol blue stain- mast and eos staining Message-ID: <4a722ef70802040124v769b79b5qddba8fb8125800a0@mail.gmail.com> Dear Histonetters, Can anyone give me a protocol for mast cell + eosinophil staining using both congo red and toluidine blue? I understand this is called the Tarpley method. Thanks, Moran -- Moran Elishmereni Department of Pharmacology and Experimental Therapeutics School of Pharmacy, Faculty of Medicine The Hebrew University of Jerusalem POB 12065 Jerusalem 91120, ISRAEL Tel: 972-2-675-8746 Fax: 972-2-675-8144 Email: moran.elish@gmail.com From tora.bardal <@t> bio.ntnu.no Mon Feb 4 06:38:34 2008 From: tora.bardal <@t> bio.ntnu.no (Tora Bardal) Date: Mon Feb 4 06:38:54 2008 Subject: [Histonet] automated in situ detection Message-ID: <47A7074A.6020301@bio.ntnu.no> Hello all I would appreciate your recommendation on automated systems for in situ hybridization, slides and whole mount. Tora -- ------------------------------------------------------------------------ Tora Bardal NTNU Senter for fiskeri og havbruk /NTNU Center of Fisheries and Aquaculture NTNU, 7491 Trondheim Norway From srishan <@t> mail.holyname.org Mon Feb 4 07:43:41 2008 From: srishan <@t> mail.holyname.org (srishan@mail.holyname.org) Date: Mon Feb 4 07:44:25 2008 Subject: [Histonet] regarding competency assessment for histotech Message-ID: Hi All, I am in the process of revising my competency assessment for the histotechs in the department. How is every one doing this process and how many times is it done during a year? Currently it is done by direct observation and incoperating testing the techs are doing for the HQIP program. Any other pointers? Thanks Nirmala Srishan Histology Supervisor Holy Name Hospital Teaneck NJ 07666 From hstevens3 <@t> mail.gatech.edu Mon Feb 4 08:16:38 2008 From: hstevens3 <@t> mail.gatech.edu (Hazel Stevens) Date: Mon Feb 4 08:17:06 2008 Subject: [Histonet] bone from cartilage Message-ID: <002901c86738$9843aa60$c8caff20$@gatech.edu> Hi Histonet , Wondered if anyone could help. I'm looking for a stain that distinguishes cartilage (calcified or not) from bone in decalcified growth plate sections (MMA). Does such a stain exist? Many thanks Hazel Hazel Stevens, Temporary Research Scientist, Georgia Tech From tp2 <@t> medicine.wisc.edu Mon Feb 4 08:49:26 2008 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Mon Feb 4 08:49:59 2008 Subject: [Histonet] Tissue Micro Arrays Message-ID: <47A6D196020000DF00005644@gwmail.medicine.wisc.edu> I typically use a vertical line of 3 tonsil cores to denote the upper lefthand corner of the array. Tom Pier >>> Thom Jensen 02/03/08 8:11 PM >>> Hey all, I am trying to compile proven methods Array Technicians have use tissue markers to help locate the first position of an array. I have used tonsil and other control tissues for location markers. But I mostly uses died lung tissues. This is how we distinguish one group of 100 cores from another. Each group has a different color location marker. This is what our doctor's have preferred. Any one else want to share other ideas or ways they have made location markers for TMAs . Thanks a bunch, Thom Histologist/TMA Technician For Free TMA instruction go to: www.arrayworkshop.com For New TMA Instrument go to: www.arraymold.com _________________________________________________________________ Helping your favorite cause is as easy as instant messaging. You IM, we give. http://im.live.com/Messenger/IM/Home/?source=text_hotmail_join_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tissuearray <@t> hotmail.com Mon Feb 4 09:14:26 2008 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Mon Feb 4 09:14:43 2008 Subject: [Histonet] Tissue Micro Arrays In-Reply-To: <47A6D196020000DF00005644@gwmail.medicine.wisc.edu> References: <47A6D196020000DF00005644@gwmail.medicine.wisc.edu> Message-ID: Thanks Tom, Tonsil controls seem to be the standard by most techs. Cheers, Thom> Date: Mon, 4 Feb 2008 08:49:26 -0600> From: tp2@medicine.wisc.edu> To: tissuearray@hotmail.com; histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Tissue Micro Arrays> > I typically use a vertical line of 3 tonsil cores to denote the upper lefthand corner of the array.> > Tom Pier> > >>> Thom Jensen 02/03/08 8:11 PM >>>> > Hey all,> I am trying to compile proven methods Array Technicians have use tissue markers to help locate the first position of an array. I have used tonsil and other control tissues for location markers. But I mostly uses dyed lung tissues. This is how we distinguish one group of 100 cores from another. Each group has a different color location marker. This is what our doctor's have preferred.> > Any one else want to share other ideas or ways they have made location markers for TMAs .> > Thanks a bunch,> Thom> Histologist/TMA Technician> > > > For Free TMA instruction go to: www.arrayworkshop.com> > For New TMA Instrument go to: www.arraymold.com> > > > _________________________________________________________________> Helping your favorite cause is as easy as instant messaging. You IM, we give.> http://im.live.com/Messenger/IM/Home/?source=text_hotmail_join_______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> _________________________________________________________________ Climb to the top of the charts!?Play the word scramble challenge with star power. http://club.live.com/star_shuffle.aspx?icid=starshuffle_wlmailtextlink_jan From froyer <@t> bitstream.net Mon Feb 4 09:19:18 2008 From: froyer <@t> bitstream.net (Ford Royer) Date: Mon Feb 4 09:19:40 2008 Subject: [Histonet] Surpath prep-stain Slide Processor Message-ID: <00a101c86741$500c7cf0$7701a80a@Ford> Second attempt to send... (I got a note that the original had not gone through) ______________ F.Y.I. to all interested parties... I am posting this for a pathologist who will be retiring from private practice in a few months. They have a complete SurPath PrepMate PrepStain Slide Processing system for liquid-based Pap Testing that they would like to sell. Includes many accessories. Any interested parties can contact me off-the List. Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL: 612-839-1046 Phone: 763-542-8725 Fax: 763-546-4830 eMail: froyer@bitstream.net From rjbuesa <@t> yahoo.com Mon Feb 4 09:51:20 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Feb 4 09:51:35 2008 Subject: [Histonet] regarding competency assessment for histotech In-Reply-To: Message-ID: <386476.62080.qm@web61218.mail.yahoo.com> I used to review competencies annually, mostly to "hone them", but if a new procedure or protocol phase changes, the competencies have to be updated. Ren? J. srishan@mail.holyname.org wrote: Hi All, I am in the process of revising my competency assessment for the histotechs in the department. How is every one doing this process and how many times is it done during a year? Currently it is done by direct observation and incoperating testing the techs are doing for the HQIP program. Any other pointers? Thanks Nirmala Srishan Histology Supervisor Holy Name Hospital Teaneck NJ 07666 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From rjbuesa <@t> yahoo.com Mon Feb 4 09:52:09 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Feb 4 09:52:24 2008 Subject: [Histonet] bone from cartilage In-Reply-To: <002901c86738$9843aa60$c8caff20$@gatech.edu> Message-ID: <884215.79703.qm@web61211.mail.yahoo.com> Use Verhoeff''s orcein. Ren? J. Hazel Stevens wrote: Hi Histonet , Wondered if anyone could help. I'm looking for a stain that distinguishes cartilage (calcified or not) from bone in decalcified growth plate sections (MMA). Does such a stain exist? Many thanks Hazel Hazel Stevens, Temporary Research Scientist, Georgia Tech _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From mona_diane <@t> hotmail.com Mon Feb 4 10:12:42 2008 From: mona_diane <@t> hotmail.com (Ramona Turner) Date: Mon Feb 4 10:12:56 2008 Subject: [Histonet] Amyloid Control Message-ID: I am looking for a really good amyloid control slide that I can purchase. I tried Sigma and was not impressed. Can anyone suggest an EXCELLENT control that I can purchase? Ramona Turner, HT (ASCP) Histology/Cytology Supervisor Potomac Hospital 2300 Opitz Blvd. Woodbridge, VA 22191 _________________________________________________________________ Connect and share in new ways with Windows Live. http://www.windowslive.com/share.html?ocid=TXT_TAGHM_Wave2_sharelife_012008 From MThiel <@t> lexpharma.com Mon Feb 4 10:19:22 2008 From: MThiel <@t> lexpharma.com (Thiel, Mary) Date: Mon Feb 4 10:19:33 2008 Subject: [Histonet] Mitochondria staining Message-ID: <7503733FE451D3479B6E8BBB554B1C5B0182A5A6@wdexchmb01.lexicon.lexgen.com> Does anyone out there have a good stain (besides Cain's) for mitochondria? Thanks, Mary The contents of this communication, including any attachments, may be confidential, privileged or otherwise protected from disclosure. They are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please do not read, copy, use or disclose the contents of this communication. Please notify the sender immediately and delete the communication in its entirety. From JGarfield <@t> lifecell.com Mon Feb 4 10:24:11 2008 From: JGarfield <@t> lifecell.com (Jacqueline D. Garfield) Date: Mon Feb 4 10:24:36 2008 Subject: [Histonet] Amyloid Control Message-ID: Try Mercedes Medical. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ramona Turner Sent: Monday, February 04, 2008 11:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Amyloid Control I am looking for a really good amyloid control slide that I can purchase. I tried Sigma and was not impressed. Can anyone suggest an EXCELLENT control that I can purchase? Ramona Turner, HT (ASCP) Histology/Cytology Supervisor Potomac Hospital 2300 Opitz Blvd. Woodbridge, VA 22191 _________________________________________________________________ Connect and share in new ways with Windows Live. http://www.windowslive.com/share.html?ocid=TXT_TAGHM_Wave2_sharelife_012 008_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *********************************************************************************************************************************************** This e-mail message and any attachments are confidential. Dissemination, distribution or copying of this e-mail or any attachments by anyone other than the intended recipient is prohibited. If you are not the intended recipient, please notify LifeCell Corporation immediately by replying to this e-mail, and destroy all copies of this e-mail and any attachments. Thank you! *********************************************************************************************************************************************** From LSebree <@t> uwhealth.org Mon Feb 4 07:38:03 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Mon Feb 4 10:35:26 2008 Subject: [Histonet] Yikes!!! Message-ID: Yeah so I thought, because my co-worker is off, I'd get a head start on the work this Monday morning so I came in early ....and how do I get rewarded for my dedication? CAP shows up! Wish me luck. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From tora.bardal <@t> bio.ntnu.no Mon Feb 4 04:13:43 2008 From: tora.bardal <@t> bio.ntnu.no (Tora Bardal) Date: Mon Feb 4 10:35:32 2008 Subject: [Histonet] automated in situ detection Message-ID: <47A6E557.60208@bio.ntnu.no> Hello all I would appreciate your recommendation on automated systems for in situ hybridization, slides and whole mount. Tora -- ------------------------------------------------------------------------ Tora Bardal NTNU Senter for fiskeri og havbruk /NTNU Center of Fisheries and Aquaculture NTNU, 7491 Trondheim Norway From liz <@t> premierlab.com Mon Feb 4 10:38:10 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Mon Feb 4 10:38:28 2008 Subject: [Histonet] Mitochondria staining In-Reply-To: <0F6E5BD8C8CA4963893BB9838CF8885A@PremierLab.local> References: <0F6E5BD8C8CA4963893BB9838CF8885A@PremierLab.local> Message-ID: Mary There is a human anti-mitochondrial antibody that works nicely. I have a protocol if you need one. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thiel, Mary Sent: Monday, February 04, 2008 9:30 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Mitochondria staining Does anyone out there have a good stain (besides Cain's) for mitochondria? Thanks, Mary The contents of this communication, including any attachments, may be confidential, privileged or otherwise protected from disclosure. They are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please do not read, copy, use or disclose the contents of this communication. Please notify the sender immediately and delete the communication in its entirety. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> CDC.GOV Mon Feb 4 10:48:40 2008 From: jqb7 <@t> CDC.GOV (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Mon Feb 4 10:49:03 2008 Subject: [Histonet] Yikes!!! In-Reply-To: References: Message-ID: <34BB307EFC9A65429BBB49E330675F72045E24A2@LTA3VS003.ees.hhs.gov> No good deed............... Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A. Sent: Monday, February 04, 2008 8:38 AM To: Histonet Subject: [Histonet] Yikes!!! Yeah so I thought, because my co-worker is off, I'd get a head start on the work this Monday morning so I came in early ....and how do I get rewarded for my dedication? CAP shows up! Wish me luck. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mauger <@t> email.chop.edu Mon Feb 4 11:03:00 2008 From: mauger <@t> email.chop.edu (Joanne Mauger) Date: Mon Feb 4 11:04:04 2008 Subject: [Histonet] automated in situ detection Message-ID: Tora, We are using the Bondmax from Visionbiosystems(now Leica). It works great for ISH on slides. Jo Mauger From vic <@t> vetmed.wsu.edu Mon Feb 4 11:55:01 2008 From: vic <@t> vetmed.wsu.edu (Leyva-Grado, Victor) Date: Mon Feb 4 11:55:14 2008 Subject: [Histonet] IL1 KO mouse and cytokines in neurons In-Reply-To: References: Message-ID: <34EFB08480241347BEBE1F095ABB96F420782D@cvm36.vetmed.wsu.edu> Dear Histonetters, I'm working with mice brains and virus infection. I evaluate the presence of cytokines in the olfactory bulb and found that with the antibody I'm using R&D for TNF and Millipore (Chemicon) for IL1b not only some glial cells are stained but also some neurons are stained. However, the production of cytokines in neurons is for some people controversial and I have been asked for the reviewers to characterize the antibodies I'm using including doing IHC on brain tissues of KO mice, so I have two questions: Have you ever have the experience with neurons being immunoreactive to TNF alpha or IL1 beta? I'm using DAB staining and double label with NeuN. I know that Jackson Labs have a TNF KO strain, but do you know of any Lab that has the IL1 beta KO's? I know they're not comercially available. Thanks a lot. Victor Leyva Washington State University From CIngles <@t> uwhealth.org Mon Feb 4 12:08:10 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Mon Feb 4 12:10:27 2008 Subject: [Histonet] Re: Alcian Blue Quality Control OT References: Message-ID: <08A0A863637F1349BBFD83A96B27A50A1200DB@uwhis-xchng3.uwhis.hosp.wisc.edu> In the personal opinion of this grumpy old pathologist - if I have 20 cases to sign out today, with one gastric biopsy, I'm quite happy to have a toluidine blue or Giemsa or Diff-Quik II stain, and spend a few minutes going over it with an oil immersion lens. If I have to sign out 70 cases with 10 gastric biopsies, I want an immunostain for H.p. Bob Richmond Samurai Pathologist Knoxville TN So That's what GOP means! ;) Claire From MadaryJ <@t> MedImmune.com Mon Feb 4 12:19:00 2008 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Mon Feb 4 12:19:27 2008 Subject: [Histonet] citrisolce and H&E quality, help please? Message-ID: Hey histo experts(I used to think I was one, but I gave it up), Okay I started working part time in this lab that has been empty for a couple of months. Stuff sitting in barely enough NBF. They do not have much to work with as far as getting me a bunch of new stuff right now, even expendables. So I combined the stuff in the 2 processors, and rotated a couple of alcohols, clearing, paraffins, and did a run, cut and everything was fine except I had to use a xylene sub. My problem is I used an aliquot of hematoxylin from a batch I had been using with success, and made of fresh eosin and the stains just looked average. Little eosin phloxine pick up and yes I used acetic but most did not even pick up. The only difference in what I did at this new place is the new place uses citrisolve in all the processing, depar, thru coverslipping. I found enough xylene to use for covering, but I was just not happy with the stain. And all the stain line reagents were from new containers. I opened up new everything. I have not used Hemo de since the 80's. I hate subs personally. Terpenes are a neurotoxin and although I cannot do much more damage and would like to use the 5 neurons I have left, one for processing, embedding, cutting, staining and coffee breaks. So can this citrisolve stuff be the problem? Can even processing in it and depar mean I need to use something different for my HE, times, formulas? I am frustrated to say the lease, but I just started so I have time to figure things out. In the meantime any help would be appreciated. Nick Madary, HT/HTL(ASCP)QIHC Histology Mgr, Medimmune 301.398.6113(lab), 4745(vm),9745(fax) "To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation." From angela.mcnabola <@t> ikonisys.com Mon Feb 4 12:19:51 2008 From: angela.mcnabola <@t> ikonisys.com (Angela McNabola) Date: Mon Feb 4 12:20:01 2008 Subject: [Histonet] FISH/ISH on Archival slides Message-ID: <4ECD18F12E443644B1F3C924A2039824608D2D@ikoexchange.ikonisys.com> Hi all, I'm a "former" histotech performing FISH on cytology samples (primarily cervical). I have been given archival slides, 2-7 years old, that have been Pap stained. As you can imagine, they are not necessarily optimal samples. Some are even coverslipped with the "tape" method, and it is taking days to soak it off. I have tried a few variations of destaining, but the problem is that I'm not getting any FISH signals at all. Basically we are using a 7 centromere or 11q (commercially obtained). Does anybody have any insight, thoughts, or ideas on how to get this to work? Thanks! Angela Angela McNabola, MS HT(ASCP)SLS, QIHC Research Scientist Ikonisys, Inc. 5 Science Park New Haven, CT 06511 203-776-0791 angela.mcnabola@ikonisys.com From jmahoney <@t> alegent.org Mon Feb 4 12:35:49 2008 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Mon Feb 4 12:36:18 2008 Subject: [Histonet] FISH/ISH on Archival slides In-Reply-To: <4ECD18F12E443644B1F3C924A2039824608D2D@ikoexchange.ikonisys.com> References: <4ECD18F12E443644B1F3C924A2039824608D2D@ikoexchange.ikonisys.com> Message-ID: <47A706A50200003C0002A0B9@gwia.alegent.org> Acetone removes tape coverfilm. It should only take a few minutes. Jan Mahoney Alegent Health Omaha >>> Angela McNabola 02/04/2008 12:19 PM >>> Hi all, I'm a "former" histotech performing FISH on cytology samples (primarily cervical). I have been given archival slides, 2-7 years old, that have been Pap stained. As you can imagine, they are not necessarily optimal samples. Some are even coverslipped with the "tape" method, and it is taking days to soak it off. I have tried a few variations of destaining, but the problem is that I'm not getting any FISH signals at all. Basically we are using a 7 centromere or 11q (commercially obtained). Does anybody have any insight, thoughts, or ideas on how to get this to work? Thanks! Angela Angela McNabola, MS HT(ASCP)SLS, QIHC Research Scientist Ikonisys, Inc. 5 Science Park New Haven, CT 06511 203-776-0791 angela.mcnabola@ikonisys.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jason.Burrill <@t> crl.com Mon Feb 4 12:44:04 2008 From: Jason.Burrill <@t> crl.com (Burrill, Jason) Date: Mon Feb 4 12:44:34 2008 Subject: [Histonet] Disposal of waste 10% NBF In Texas Message-ID: <1AD4E907E9B6F648AEF1B3A20A9B0E1EB270D5@shr-exch2.na01.crl.com> I am preparing my safety workshop I am giving in Dallas for the Texas Society for Histotechnology In April and I was trying to determine how Texas classifies waste 10% neutral buffered formalin, this is a question that is commonly asked in my workshop. I like to familiarize myself with the state regulations if I am providing the training in a particular state so I e-mailed the Texas Commission of Environmental Quality and am awaiting a clarified response to their first answer. Would anyone mind sharing your lab's policy for handling of waste 10% NBF? If you prefer to respond to me directly I will keep all responses confidential. In my lab we collect it as Non-hazardous waste and it is sent to a water treatment facility for processing. Thanks in advance, Jason Jason Burrill Sr. Manager, Histology and Laboratory Safety Charles River Laboratories 251 Ballardvale Street Wilmington, MA 01887 Ph: 781-222-6152 Fax: 978-988-8793 jason.burrill@crl.com **Please note new direct dial telephone number** From gayle.callis <@t> bresnan.net Mon Feb 4 12:58:01 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Mon Feb 4 12:58:13 2008 Subject: [Histonet] bone from cartilage References: <884215.79703.qm@web61211.mail.yahoo.com> Message-ID: <006b01c8675f$dd82f3c0$6601a8c0@Sunney> Haxel, Toluidine Blue should do it. Eurell JA, Sterchi DL. Microwaveable toluidine blue stain for surface staining of undecalcified bone sections, J Histotechnology, Decembetr 1994. Diane Sterchi developed this stain using pH 8 phosphate buffer and by heating the staining solution using a microwave. Just don't microwave the section while it is in the stain. It is easy to do. If you are a member of NSH and do not have the journal, you should be able to access the publication without fee. Also, a combination of this T blue and MacNeal's tetrachrome. It will be a more the stains tinctorial differences of the articular versus the calcified cartilage that you need to get used too. The stain works on both ground, surface stained thick sections or microtomed MMA sections. The Verhoeff's orcein may work but only if you totally remove the MMA from a microtomed section using a solvent, hot xylene is one that works. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Rene J Buesa" To: "Hazel Stevens" ; Sent: Monday, February 04, 2008 8:52 AM Subject: Re: [Histonet] bone from cartilage > Use Verhoeff''s orcein. > Ren? J. > > Hazel Stevens wrote: > Hi Histonet , > > > > Wondered if anyone could help. I'm looking for a stain that distinguishes > cartilage (calcified or not) from bone in decalcified growth plate > sections > (MMA). Does such a stain exist? > > > > Many thanks > > Hazel > > > > Hazel Stevens, > > Temporary Research Scientist, > > Georgia Tech > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Never miss a thing. Make Yahoo your homepage. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AGrobe2555 <@t> aol.com Mon Feb 4 13:51:21 2008 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Mon Feb 4 13:51:41 2008 Subject: [Histonet] Citrisolve & HE Message-ID: We used xylene for tissue processing/embedding and Citrisolve when doing the H&E stain. The slides are de-waxed and ultimately cover-slipped with Citrisolve. I have not seen any effect on staining intensity. Albert Albert C. Grobe, PhD Tissue Engineering Lab International Heart Institute of Montana Foundation **************Biggest Grammy Award surprises of all time on AOL Music. (http://music.aol.com/grammys/pictures/never-won-a-grammy?NCID=aolcmp003000000025 48) From rjbuesa <@t> yahoo.com Mon Feb 4 14:21:46 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Feb 4 14:21:57 2008 Subject: [Histonet] Mitochondria staining In-Reply-To: <7503733FE451D3479B6E8BBB554B1C5B0182A5A6@wdexchmb01.lexicon.lexgen.com> Message-ID: <20036.29174.qm@web61220.mail.yahoo.com> A good Mallory-Azan in a very well fixed/processed tissue in a thin section stains mitochondria superbly. Ren? J. "Thiel, Mary" wrote: Does anyone out there have a good stain (besides Cain's) for mitochondria? Thanks, Mary The contents of this communication, including any attachments, may be confidential, privileged or otherwise protected from disclosure. They are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please do not read, copy, use or disclose the contents of this communication. Please notify the sender immediately and delete the communication in its entirety. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From detmar <@t> mshri.on.ca Mon Feb 4 14:50:36 2008 From: detmar <@t> mshri.on.ca (Jacqui Detmar) Date: Mon Feb 4 14:51:00 2008 Subject: [Histonet] IL1 KO mouse and cytokines in neurons In-Reply-To: <34EFB08480241347BEBE1F095ABB96F420782D@cvm36.vetmed.wsu.edu> References: <34EFB08480241347BEBE1F095ABB96F420782D@cvm36.vetmed.wsu.edu> Message-ID: Hi Victor. IL-1alpha was knocked out by Iwakura's group in 1998 (see Horai et al., 1998). IL-1beta was knocked out by David Chaplin's group (see Shornick et al., 1996) and Lex Van der Ploeg's group (see Zheng et al., 1995). Have you tried depleting the antibody with the peptide immunogen? Is this even possible for these antibodies? Just a thought. Good luck mousing. Jacqui Detmar, Post-doctoral Fellow Samuel Lunenfeld Research Institute, room 876 Mount Sinai Hospital 600 University Avenue Toronto, ON, Canada M5G 1X5 phone: 416-586-4800 x2451/x2290 fax: 416-586-8588 email: detmar@mshri.on.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LuckG <@t> empirehealth.org Mon Feb 4 14:59:59 2008 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Mon Feb 4 15:00:09 2008 Subject: [Histonet] FW: CAP Competency Question Message-ID: <6BB8BC4519AAB844B174FC739A679BBCCF000F@IRMEXCH01.irm.inhs.org> > Subject: CAP Competency Question > > Ok everyone, here is the CAP question that I am most concerned about. > I'd like to see an example of how each of you intend to answer this > question by Friday, Feb. 15. I do not want a reply by email...I do > want actual documentation that I can put into my manual to help answer > the inspector's question. Thanks. > > GEN.55500 Phase II N/A YES NO > > Has the competency of each person to perform his/her assigned duties > been assessed? > > NOTE: The manual that describes training activities and evaluations > must be specific for each job description. Those activities requiring > judgment or interpretive skills must be included. The records must > make it possible for the inspector to determine what skills were > assessed and how those skills were measured. The competency of each > person to perform the duties assigned must be assessed following > training, and at least annually thereafter. During the first year > that an individual tests patient specimens, competency must be > assessed at least every six months. Retraining and reassessment of > employee competency must occur when problems are identified with > employee performance. Competency assessment for each individual must > include all of the following elements that are applicable to the > individual's duties: > > 1. Direct observations of routine patient test performance, > including patient preparation, if applicable, specimen handling, > processing and testing > 2. Monitoring the recording and reporting of test results > 3. Review of intermediate test results or worksheets, quality > control records, proficiency testing results, and preventive > maintenance records > 4. Direct observation of performance of instrument maintenance and > function checks > 5. Assessment of test performance through testing previously > analyzed specimens, internal blind testing samples or external > proficiency testing samples; and > 6. Evaluation of problem-solving skills > > From KLAPANO1 <@t> hfhs.org Mon Feb 4 15:02:04 2008 From: KLAPANO1 <@t> hfhs.org (Karen Lapanowski) Date: Mon Feb 4 15:02:43 2008 Subject: [Histonet] Masson Trichrome Stain on Frozen sections Message-ID: Hello, I'm looking for a protocol for using a Masson Trichrome stain kit on frozen-fixed section. Any help? Thanks, Karen ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== From koellingr <@t> comcast.net Mon Feb 4 16:39:23 2008 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Mon Feb 4 16:39:30 2008 Subject: [Histonet] IL1 KO mouse and cytokines in neurons Message-ID: <020420082239.9958.47A7941A000DA756000026E622007354469D09020704040A0105@comcast.net> Victor and Jacqui, Could "some" neurons appearing to be immunoreactive to TNF alpha or Il-1 beta simply be the fact that they are cytokines? Small molecular weight, they are meant to be released from cells and not membrane bound there or as part of the cell structure. Glial cells, by the very fact of what they are, are intimately associated with neurons. How was tissue fixed? Maybe they are dispersed or leaking to a neighboring neuron. Many targets are anchored where they are, such as your NeuN label, and that target is not going anywhere. Cytokines do. The references of 10-15 years ago were novel then. Not so now. TNK K/o as you said you can get. At Wash State, you should have a good mouse transgenics and mouse k/o husbandry facility. With some Balb/c's, they should be able to generate IL-1 alpha -/-. beta -/- or alpha/beta double knock/outs. Not that difficult for a good knock out genetics lab. If you are using the knock outs as control for the IHC staining (and this applies to any use of knock-outs as negative controls for any IHC staining) be sure you know EXACTLY what is knocked out. A mouse frizzle/frazzle knock-out does not imply the total absence of mouse frizzle/frazzel gene and protein. One particular coding exon in a multi-exon gene could leave the protein unresponsive or unusable in-vivo or shortened but enough might be left, and if the proper epitope is left, for IHC staining to occur. Knock out is not the same as complete absence. So you can stain something that is "knocked out" and that is why you need to be aware of EXACTLY what is knocked out. Many people, including yours truely, has been caught like this. Ray -------------- Original message -------------- From: "Jacqui Detmar" > Hi Victor. > > IL-1alpha was knocked out by Iwakura's group in 1998 (see Horai et al., > 1998). IL-1beta was knocked out by David Chaplin's group (see Shornick > et al., 1996) and Lex Van der Ploeg's group (see Zheng et al., 1995). > Have you tried depleting the antibody with the peptide immunogen? Is > this even possible for these antibodies? Just a thought. Good luck > mousing. > > Jacqui Detmar, Post-doctoral Fellow > Samuel Lunenfeld Research Institute, room 876 > Mount Sinai Hospital > 600 University Avenue > Toronto, ON, Canada > M5G 1X5 > > phone: 416-586-4800 x2451/x2290 > fax: 416-586-8588 > email: detmar@mshri.on.ca > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From barbaraaalbert <@t> yahoo.com Mon Feb 4 17:33:30 2008 From: barbaraaalbert <@t> yahoo.com (Barbara Albert) Date: Mon Feb 4 17:33:37 2008 Subject: [Histonet] Storage codes Message-ID: <816222.1760.qm@web63711.mail.re1.yahoo.com> Hi all, I just received some vials of Gold Chloride that I had ordered and was checking the label for stoarge requirements. It has "Storage Code White". I haven't heard of storage codes and want to know where I can go to find out what they mean. Thanks, Barbara Albert UCSF Medical Center San Francisco --------------------------------- Never miss a thing. Make Yahoo your homepage. From Luis.Chiriboga <@t> med.nyu.edu Tue Feb 5 08:08:48 2008 From: Luis.Chiriboga <@t> med.nyu.edu (Luis Chiriboga) Date: Tue Feb 5 08:09:11 2008 Subject: [Histonet] NYSHS 2008 Symposium Program Message-ID: is available on the New York State Histotechnological Society website. Please visit www.nyhisto.org Hope to see you there From SARAH.REEVES <@t> ekht.nhs.uk Tue Feb 5 07:25:00 2008 From: SARAH.REEVES <@t> ekht.nhs.uk (SARAH REEVES) Date: Tue Feb 5 08:25:56 2008 Subject: [Histonet] (no subject) Message-ID: <20080205T142533Z_51D000000000@ekht.nhs.uk> Hi, Has anyone had dealings with the Ventana Special Stainer? Just wondered what thoughts people had about it. Thanks ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of East Kent Hospitals NHS Trust. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you have received this email in error please notify the system manager at the following email address: root.postmaster@ekht.nhs.uk www.kentandmedway.nhs.uk This footnote also confirms that although this email message has been swept by MIMEsweeper for the presence of computer viruses, it is strongly recommended that you carry out your own virus scan of this message and any attachments. www.mimesweeper.com ********************************************************************** From doug <@t> ppspath.com Tue Feb 5 08:48:21 2008 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Feb 5 08:49:10 2008 Subject: SPAM-LOW: [Histonet] (no subject) In-Reply-To: <20080205T142533Z_51D000000000@ekht.nhs.uk> Message-ID: Sarah, I have used the stainer for about 10 years. Pros - It is a workhorse. It is easy to maintain and operate. Staining is consistent. Cons - Stain menu. I would like to see a wider variety of stains and more dilatability with the protocols. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of SARAH REEVES Sent: Tuesday, February 05, 2008 8:25 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] (no subject) Hi, Has anyone had dealings with the Ventana Special Stainer? Just wondered what thoughts people had about it. Thanks ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of East Kent Hospitals NHS Trust. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you have received this email in error please notify the system manager at the following email address: root.postmaster@ekht.nhs.uk www.kentandmedway.nhs.uk This footnote also confirms that although this email message has been swept by MIMEsweeper for the presence of computer viruses, it is strongly recommended that you carry out your own virus scan of this message and any attachments. www.mimesweeper.com ********************************************************************** From mpence <@t> grhs.net Tue Feb 5 09:43:26 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Feb 5 09:43:32 2008 Subject: SPAM-LOW: [Histonet] (no subject) In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C8AE@IS-E2K3.grhs.net> I second what Doug says. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, February 05, 2008 8:48 AM To: 'SARAH REEVES'; histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: [Histonet] (no subject) Sarah, I have used the stainer for about 10 years. Pros - It is a workhorse. It is easy to maintain and operate. Staining is consistent. Cons - Stain menu. I would like to see a wider variety of stains and more dilatability with the protocols. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of SARAH REEVES Sent: Tuesday, February 05, 2008 8:25 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] (no subject) Hi, Has anyone had dealings with the Ventana Special Stainer? Just wondered what thoughts people had about it. Thanks ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of East Kent Hospitals NHS Trust. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you have received this email in error please notify the system manager at the following email address: root.postmaster@ekht.nhs.uk www.kentandmedway.nhs.uk This footnote also confirms that although this email message has been swept by MIMEsweeper for the presence of computer viruses, it is strongly recommended that you carry out your own virus scan of this message and any attachments. www.mimesweeper.com ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmahoney <@t> alegent.org Tue Feb 5 10:20:49 2008 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Tue Feb 5 10:21:12 2008 Subject: [Histonet] Ventana Special Stainer In-Reply-To: <20080205T142533Z_51D000000000@ekht.nhs.uk> References: <20080205T142533Z_51D000000000@ekht.nhs.uk> Message-ID: <47A838810200003C0002A269@gwia.alegent.org> Hi Sarah, We have been using the same Nexis instrument for almost 6 years. The stainer was a demo when we bought it. We put it through the paces. The only issue has been some inconsistency with silvers but that was finally resolved to our satisfaction. I also would like to see a gram stain on the platform. It is a very user friendly instrument . I hope to see hydrating and dehydrating on the next generation. Other than that is it completely "walk away". Jan Mahoney Alegent Health Omaha NE >>> SARAH REEVES 02/05/2008 7:25 AM >>> Hi, Has anyone had dealings with the Ventana Special Stainer? Just wondered what thoughts people had about it. Thanks From olek.michalski <@t> nencki.gov.pl Tue Feb 5 11:59:33 2008 From: olek.michalski <@t> nencki.gov.pl (Olek Michalski) Date: Tue Feb 5 11:53:33 2008 Subject: [Histonet] sections falling off Message-ID: Dear Histonetters, a friend of mine just faced a massive sections falling off. She is doing Nissl staining in mouse brain (brains perfused with formalin, postfixed 3 days, and cryosectioned) on poly-L-lysined slides. She just managed to pass trough xylene and decreasing grades of alcohol to water and the sections started to detach. We were trying to reattach them but it went out that sections are not flat any more. It seems like the tissue is rehydrated unevenly, but keeping it in water for hours didn't work. Is there any way to spread these sections not damaging them at the same time? She is likely to rescue this sections even if some work is needed. Best regards Olek Michalski -- Laboratory of Neurobiology of Development and Evolution Nencki Institute of Experimental Biology ul. Pasteura 3, 02-093 Warszawa, Poland Tel. +48 22 5892268, Fax +48 22 8225342 From cforster <@t> umn.edu Tue Feb 5 12:20:37 2008 From: cforster <@t> umn.edu (Colleen Forster) Date: Tue Feb 5 12:18:40 2008 Subject: [Histonet] EpoR antibody Message-ID: <47A8A8F5.3090606@umn.edu> Hello histonetters, Is anyone doing the EpoR antibody on FFPE for IHC? If so, could you share the vendor and a starting dilution.....thanks. Colleen Forster U of MN From Beatrice.Debrosse-Serra <@t> pfizer.com Tue Feb 5 12:30:10 2008 From: Beatrice.Debrosse-Serra <@t> pfizer.com (Debrosse-Serra, Beatrice) Date: Tue Feb 5 12:30:21 2008 Subject: [Histonet] sections falling off In-Reply-To: Message-ID: <8404DFBED5207B4B8E5EEF4332CEEA5305BD4C99@lajamrexm01.amer.pfizer.com> Try Newcomers silane coated slides. They are not cheap, but the sections stay on very well. Beatrice DeBrosse-Serra Pathology Scientist Pfizer Global Research & Development CB4, 2150 10646 Science Center Drive San Diego, CA 92121 Phone# 858-622-5986 Fax# 858-678-8290 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Olek Michalski Sent: Tuesday, February 05, 2008 10:00 AM To: Histonet Subject: [Histonet] sections falling off Dear Histonetters, a friend of mine just faced a massive sections falling off. She is doing Nissl staining in mouse brain (brains perfused with formalin, postfixed 3 days, and cryosectioned) on poly-L-lysined slides. She just managed to pass trough xylene and decreasing grades of alcohol to water and the sections started to detach. We were trying to reattach them but it went out that sections are not flat any more. It seems like the tissue is rehydrated unevenly, but keeping it in water for hours didn't work. Is there any way to spread these sections not damaging them at the same time? She is likely to rescue this sections even if some work is needed. Best regards Olek Michalski -- Laboratory of Neurobiology of Development and Evolution Nencki Institute of Experimental Biology ul. Pasteura 3, 02-093 Warszawa, Poland Tel. +48 22 5892268, Fax +48 22 8225342 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Feb 5 12:30:54 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 5 12:31:03 2008 Subject: [Histonet] sections falling off In-Reply-To: Message-ID: <87465.99172.qm@web61214.mail.yahoo.com> If they were cryosectioned she did not have to go through xylene or graded alcohols to "dewax and hydrate" since there was no dehydration or wax infiltration to begin with. Cryosectioned sections just need to wash away the medium used to cryosection (OCT perhaps?) and just stain with the aqueous solutions. Later they can de dehydrated and cleared if a permanent mount is desired. Try to float the sections in a water bath and pick them up and don't try again to use xylene or alcohols. Probably she was following a procedure for paraffin embedded tissue that should have been adapted to frozen sections and it was not. Ren? J. Olek Michalski wrote: Dear Histonetters, a friend of mine just faced a massive sections falling off. She is doing Nissl staining in mouse brain (brains perfused with formalin, postfixed 3 days, and cryosectioned) on poly-L-lysined slides. She just managed to pass trough xylene and decreasing grades of alcohol to water and the sections started to detach. We were trying to reattach them but it went out that sections are not flat any more. It seems like the tissue is rehydrated unevenly, but keeping it in water for hours didn't work. Is there any way to spread these sections not damaging them at the same time? She is likely to rescue this sections even if some work is needed. Best regards Olek Michalski -- Laboratory of Neurobiology of Development and Evolution Nencki Institute of Experimental Biology ul. Pasteura 3, 02-093 Warszawa, Poland Tel. +48 22 5892268, Fax +48 22 8225342 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From RSRICHMOND <@t> aol.com Tue Feb 5 12:45:34 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Tue Feb 5 12:45:39 2008 Subject: [Histonet] Re: Alcian Blue Quality Control OT Message-ID: Claire Ingles remarks: So that's what GOP means! Not this grumpy old pathologist - I voted for Barack Obama! Bob Richmond Samurai Pathologist Knoxville TN From gmartin <@t> marshallmedical.org Tue Feb 5 13:00:57 2008 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Tue Feb 5 13:01:12 2008 Subject: [Histonet] HP staining Message-ID: <6ED9D4252F278841A0593D3D788AF24C01ACDF72@mailsvr.MARSHMED.local> To all who responded to my e-mail concerning rapid HP staining to include a yellow back ground ... thank you very much ... my question was answered. Gray From kmerriam2003 <@t> yahoo.com Tue Feb 5 13:48:08 2008 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Tue Feb 5 13:48:13 2008 Subject: [Histonet] Slide printers (ink) Message-ID: <813260.30617.qm@web50306.mail.re2.yahoo.com> Hi everyone, I was wondering what everyone's experience was with the TBS slide printer (Shurmark-plus), the one that prints with ink? I would appreciate input, good or bad. We currently have an etcher, and we hate it, so we are looking to trade it in for something that uses ink. I have used the Leica printer in the past and have had reasonably good luck with it, but I was wondering about the printer from TBS, I don't know anyone that has one. Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From rcharles <@t> state.pa.us Tue Feb 5 13:58:42 2008 From: rcharles <@t> state.pa.us (Charles, Roger) Date: Tue Feb 5 13:59:17 2008 Subject: [Histonet] Slide printers (ink) Message-ID: <12E4E17FEF6EBE4BAE95BEB3CDCB57000943BB6E@enhbgpri04.backup> Hello, Recently we bought a sakura slide writer that uses ink to print. The only problem I have found with the ink is if you put labels over the print and then try to remove the label to verify a number error, or something like that, the ink will actually come off with the label. If you can read upside down you can still verify the number. I did run this ink thru a battery of chemicals including straight formic acid for 5 minutes and the ink did not leave the slide. I would caution in buying a Sakura slide write as it has a very unfriendly Access based program and the writer does not work with charged slides. This has been verified by me and an independent engineer sent to look at this issue. I do use a TBS cassette writer and love that so if I can get my sakura sent back I will be looking at TBS's slide writer too. Good luck Roger Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Tuesday, February 05, 2008 2:48 PM To: Histonet Subject: [Histonet] Slide printers (ink) Hi everyone, I was wondering what everyone's experience was with the TBS slide printer (Shurmark-plus), the one that prints with ink? I would appreciate input, good or bad. We currently have an etcher, and we hate it, so we are looking to trade it in for something that uses ink. I have used the Leica printer in the past and have had reasonably good luck with it, but I was wondering about the printer from TBS, I don't know anyone that has one. Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Tue Feb 5 14:25:58 2008 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Feb 5 14:26:07 2008 Subject: [Histonet] Slide printers (ink) In-Reply-To: <12E4E17FEF6EBE4BAE95BEB3CDCB57000943BB6E@enhbgpri04.backup> References: <12E4E17FEF6EBE4BAE95BEB3CDCB57000943BB6E@enhbgpri04.backup> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD320518E54D@sjhaexc02.sjha.org> We have Sakura cassette and slide writers. We print charged slides daily. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles, Roger Sent: Tuesday, February 05, 2008 2:59 PM To: Kim Merriam Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Slide printers (ink) Hello, Recently we bought a sakura slide writer that uses ink to print. The only problem I have found with the ink is if you put labels over the print and then try to remove the label to verify a number error, or something like that, the ink will actually come off with the label. If you can read upside down you can still verify the number. I did run this ink thru a battery of chemicals including straight formic acid for 5 minutes and the ink did not leave the slide. I would caution in buying a Sakura slide write as it has a very unfriendly Access based program and the writer does not work with charged slides. This has been verified by me and an independent engineer sent to look at this issue. I do use a TBS cassette writer and love that so if I can get my sakura sent back I will be looking at TBS's slide writer too. Good luck Roger Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Tuesday, February 05, 2008 2:48 PM To: Histonet Subject: [Histonet] Slide printers (ink) Hi everyone, I was wondering what everyone's experience was with the TBS slide printer (Shurmark-plus), the one that prints with ink? I would appreciate input, good or bad. We currently have an etcher, and we hate it, so we are looking to trade it in for something that uses ink. I have used the Leica printer in the past and have had reasonably good luck with it, but I was wondering about the printer from TBS, I don't know anyone that has one. Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Reuel.Cornelia <@t> tsrh.org Tue Feb 5 14:34:27 2008 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Tue Feb 5 14:34:59 2008 Subject: [Histonet] Immunoperoxidase Double staining protocol Message-ID: <47A873F3020000C50002ADF9@nwcl02.tsrh.org> I was asked to do an immunoperoxidase double staining on CD61 ,Cd42, pf4 with an unknown mouse antibody that will work on platelets. Can somebody help me or any protocol available that I can refer to. I am used of IF double stianing but not Immunoperoxidase. Thank you. Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 ******************************************************************************************************************* Texas Scottish Rite Hospital for Children is one of the nation's leading pediatric centers for the treatment of orthopedic conditions, certain related neurological disorders and learning disorders, such as dyslexia. This email transmission and/or its attachments may contain confidential health information, intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing it to any other party unless required to do so by law and is required to delete/destroy the information after its stated need has been fulfilled. If you are not the intended recipient, any disclosure, copying, distribution or action taken in reliance on the contents of this email transmission is prohibited. If you have received this information in error, please notify the sender immediately and delete this information. We appreciate your efforts to protect the children's confidential information. ******************************************************************************************************************* From ploykasek <@t> phenopath.com Tue Feb 5 14:39:50 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue Feb 5 14:42:08 2008 Subject: [Histonet] Slide printers (ink) In-Reply-To: <12E4E17FEF6EBE4BAE95BEB3CDCB57000943BB6E@enhbgpri04.backup> Message-ID: Roger- We have been using a sakura slide printer for a couple of years. I do agree with your comments about the software - not user friendly at all nor is it intuitive. I have used Access database in the past without these issues. I do not know about the labels, I don't think we've had that issue. Maybe it varies with the label. I don't quite understand your comment on charged slides, as all of the slides we put thru the printer are charged and we have no issues with that. We use charged slides (Okando Plus) from BBC and Probe-On Plus slides from ThermoFisher. What brand of slides were you using & what issue did you have with the slide printer from the usage? Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Hello, > Recently we bought a sakura slide writer that uses ink to print. The > only problem I have found with the ink is if you put labels over the > print and then try to remove the label to verify a number error, or > something like that, the ink will actually come off with the label. If > you can read upside down you can still verify the number. I did run > this ink thru a battery of chemicals including straight formic acid for > 5 minutes and the ink did not leave the slide. I would caution in > buying a Sakura slide write as it has a very unfriendly Access based > program and the writer does not work with charged slides. This has been > verified by me and an independent engineer sent to look at this issue. > I do use a TBS cassette writer and love that so if I can get my sakura > sent back I will be looking at TBS's slide writer too. > Good luck > Roger > > Roger Charles > Microbiologist > Pennsylvania Veterinary Laboratory > 2305 N Cameron St > Harrisburg, PA 17110 > 717-787-8808 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim > Merriam > Sent: Tuesday, February 05, 2008 2:48 PM > To: Histonet > Subject: [Histonet] Slide printers (ink) > > Hi everyone, > > I was wondering what everyone's experience was with the TBS slide > printer (Shurmark-plus), the one that prints with ink? I would > appreciate input, good or bad. > > We currently have an etcher, and we hate it, so we are looking to > trade it in for something that uses ink. I have used the Leica printer > in the past and have had reasonably good luck with it, but I was > wondering about the printer from TBS, I don't know anyone that has one. > > Kim > > > Kim Merriam, MA, HT(ASCP) > Cambridge, MA > > --------------------------------- > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try > it now. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From immrstambo <@t> hotmail.com Tue Feb 5 15:10:37 2008 From: immrstambo <@t> hotmail.com (Christine Tambasco) Date: Tue Feb 5 15:10:44 2008 Subject: [Histonet] Storage codes In-Reply-To: <816222.1760.qm@web63711.mail.re1.yahoo.com> References: <816222.1760.qm@web63711.mail.re1.yahoo.com> Message-ID: I always kept my gold chloride in the refrigerator. Yours is in a vial though so is it a powder? That is what I used to have I kept at room temperature. Just my advice. Christine Tambasco, HT (ASCP) St. Marys Hospital Amsterdam, NY> Date: Mon, 4 Feb 2008 15:33:30 -0800> From: barbaraaalbert@yahoo.com> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] Storage codes> > Hi all,> I just received some vials of Gold Chloride that I had ordered and was checking the label for stoarge requirements. It has "Storage Code White". I haven't heard of storage codes and want to know where I can go to find out what they mean.> > Thanks,> Barbara Albert> UCSF Medical Center> San Francisco> > > ---------------------------------> Never miss a thing. Make Yahoo your homepage.> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Climb to the top of the charts!?Play the word scramble challenge with star power. http://club.live.com/star_shuffle.aspx?icid=starshuffle_wlmailtextlink_jan From pkromund <@t> gundluth.org Tue Feb 5 15:34:42 2008 From: pkromund <@t> gundluth.org (pkromund@gundluth.org) Date: Tue Feb 5 15:34:51 2008 Subject: [Histonet] Slide printers (ink) In-Reply-To: Message-ID: Do any of these slide printers affect precut control tissue on the charged slides? Pamela Patti Loykasek To Sent by: "Charles, Roger" histonet-bounces@ , Kim Merriam lists.utsouthwest ern.edu cc histonet@lists.utsouthwestern.edu Subject 02/05/2008 02:39 Re: [Histonet] Slide printers (ink) PM Roger- We have been using a sakura slide printer for a couple of years. I do agree with your comments about the software - not user friendly at all nor is it intuitive. I have used Access database in the past without these issues. I do not know about the labels, I don't think we've had that issue. Maybe it varies with the label. I don't quite understand your comment on charged slides, as all of the slides we put thru the printer are charged and we have no issues with that. We use charged slides (Okando Plus) from BBC and Probe-On Plus slides from ThermoFisher. What brand of slides were you using & what issue did you have with the slide printer from the usage? Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Hello, > Recently we bought a sakura slide writer that uses ink to print. The > only problem I have found with the ink is if you put labels over the > print and then try to remove the label to verify a number error, or > something like that, the ink will actually come off with the label. If > you can read upside down you can still verify the number. I did run > this ink thru a battery of chemicals including straight formic acid for > 5 minutes and the ink did not leave the slide. I would caution in > buying a Sakura slide write as it has a very unfriendly Access based > program and the writer does not work with charged slides. This has been > verified by me and an independent engineer sent to look at this issue. > I do use a TBS cassette writer and love that so if I can get my sakura > sent back I will be looking at TBS's slide writer too. > Good luck > Roger > > Roger Charles > Microbiologist > Pennsylvania Veterinary Laboratory > 2305 N Cameron St > Harrisburg, PA 17110 > 717-787-8808 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim > Merriam > Sent: Tuesday, February 05, 2008 2:48 PM > To: Histonet > Subject: [Histonet] Slide printers (ink) > > Hi everyone, > > I was wondering what everyone's experience was with the TBS slide > printer (Shurmark-plus), the one that prints with ink? I would > appreciate input, good or bad. > > We currently have an etcher, and we hate it, so we are looking to > trade it in for something that uses ink. I have used the Leica printer > in the past and have had reasonably good luck with it, but I was > wondering about the printer from TBS, I don't know anyone that has one. > > Kim > > > Kim Merriam, MA, HT(ASCP) > Cambridge, MA > > --------------------------------- > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try > it now. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Feb 5 15:46:41 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 5 15:46:46 2008 Subject: [Histonet] Immunoperoxidase Double staining protocol In-Reply-To: <47A873F3020000C50002ADF9@nwcl02.tsrh.org> Message-ID: <784337.61146.qm@web61225.mail.yahoo.com> Generally speaking you will have to run the whole protocol for one of the Abs and use one chromogen (of your selection). After that, since you already did HIER and blocked the internal peroxidase, you will treat the section with the second Ab, will link/detect it and use another chromogen that ideally should have a different color. The first chromogen could be regular DAB and for the second you can use also DAB but with incorporated nickel that will give a deep/blue purple color, in contrast with the brown/reddish normal color of the DAB. The thing is that you don't need any special protocol, just your regular IHC protocol for FFPE tissue, run twice with two different chromogens. Ren? J. Reuel Cornelia wrote: I was asked to do an immunoperoxidase double staining on CD61 ,Cd42, pf4 with an unknown mouse antibody that will work on platelets. Can somebody help me or any protocol available that I can refer to. I am used of IF double stianing but not Immunoperoxidase. Thank you. Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 ******************************************************************************************************************* Texas Scottish Rite Hospital for Children is one of the nation's leading pediatric centers for the treatment of orthopedic conditions, certain related neurological disorders and learning disorders, such as dyslexia. This email transmission and/or its attachments may contain confidential health information, intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing it to any other party unless required to do so by law and is required to delete/destroy the information after its stated need has been fulfilled. If you are not the intended recipient, any disclosure, copying, distribution or action taken in reliance on the contents of this email transmission is prohibited. If you have received this information in error, please notify the sender immediately and delete this information. We appreciate your efforts to protect the children's confidential information. ******************************************************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From LSebree <@t> uwhealth.org Tue Feb 5 15:47:03 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Tue Feb 5 15:47:09 2008 Subject: [Histonet] thanks for the support and good wishes Message-ID: Hi all, Well apparently our CAP inspector took the word of our supervisor on everything pertaining to our lab, reviewed some of our slides and was out the door before the weather associated with the "winter storm warning" arrived! Some times its good to live in the land of ice and snow! Thanks for all the good luck wishes! Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From NMargaryan <@t> childrensmemorial.org Tue Feb 5 16:50:57 2008 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Tue Feb 5 16:51:48 2008 Subject: [Histonet] (no subject) Message-ID: Dear histo team, I need to stain frozen & FFPE sections for Tuj-1 (AbCam, ab53234) and FFPE sectiond for Netrin-1 (AbCam, ab39370). Any kind of suggestions or even full IHC or IF protocol is appreciated. Thanks in advance, Naira From cforster <@t> umn.edu Tue Feb 5 17:36:48 2008 From: cforster <@t> umn.edu (Colleen Forster) Date: Tue Feb 5 17:34:48 2008 Subject: [Histonet] Do mice have tonsils?? Message-ID: <47A8F310.20905@umn.edu> For any of you have done mouse necropsy, do they have tonsils or just lymph nodes? I have had a request for mouse tonsils.... can't seem to find them in my searches. I would appreciate any help on this one. Thanks, Colleen Forster U of MN From ebreisch <@t> rchsd.org Tue Feb 5 17:32:31 2008 From: ebreisch <@t> rchsd.org (Breisch, Eric) Date: Tue Feb 5 17:39:40 2008 Subject: [Histonet] eosinophils on frozen section Message-ID: <43B97B4C402C2C44AAA2A8D2C86A88B31A5A78@e2k3backend1.RCHSD.org> To all interested Histonetters: We are experiencing some frustration with our ability to identify eosinophils from frozen sections. For some reason which our frozen section set up does not enable us to identify eosinophils. Eosinophil identification is not impaired when the tissue is submitted for permanents. Could some one please share the reagents used and how the frozen section staining area is set up if they have success identifying eosinophils from a frozen section? We have had many frozen sections done on fresh tissues suspicious for an eosinophilic granuloma but our techniques so far have not rendered consistent eosinophil identification. We presently are using 95% ETOH for fixing the FS slide, quick wash in distilled H2O, 1 minute in Richard Allen Hematoxylin 2, rinse in tap water, 15 seconds in bluing, rinse in water, rinse in 70% ETOH, 1 minute in Richard Allen Eosin Y, three rinses in 95% ETOH, three rinses in Xylene substitute and then coverslip. All other tissues appear just fine but the eosinophils just don't show up. Any suggestions are greatly appreciated. Eric A. Breisch, Ph.D. Clinical Anatomist Dept. of Pathology Rady Children's Hospital and Health Center Associate Clinical Professor of Anatomy Dept. of Surgery UCSD School of Medicine From CIngles <@t> uwhealth.org Tue Feb 5 18:08:15 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Tue Feb 5 18:08:20 2008 Subject: [Histonet] eosinophils on frozen section References: <43B97B4C402C2C44AAA2A8D2C86A88B31A5A78@e2k3backend1.RCHSD.org> Message-ID: <08A0A863637F1349BBFD83A96B27A50A1200DF@uwhis-xchng3.uwhis.hosp.wisc.edu> Eric: My guess is that the alcohol is lysing(blowing up) the unfixed eosinophils, therefore they are not there to stain. Fixation stabilizes the cells in order to be stained in regular paraffin sections. Unfortunately, I have done a few trials using different fixatives for fresh tissue to see if I could preserve these little suckers. Nothing really worked satisfactorally. You might try fixing in formalin for a minute or two before you continue on with your stain and see if this helps. (don't forget to wash the sections afterward) Hope this helps. Claire We are experiencing some frustration with our ability to identify eosinophils from frozen sections. For some reason which our frozen section set up does not enable us to identify eosinophils. Eosinophil identification is not impaired when the tissue is submitted for permanents. Could some one please share the reagents used and how the frozen section staining area is set up if they have success identifying eosinophils from a frozen section? We have had many frozen sections done on fresh tissues suspicious for an eosinophilic granuloma but our techniques so far have not rendered consistent eosinophil identification. We presently are using 95% ETOH for fixing the FS slide, quick wash in distilled H2O, 1 minute in Richard Allen Hematoxylin 2, rinse in tap water, 15 seconds in bluing, rinse in water, rinse in 70% ETOH, 1 minute in Richard Allen Eosin Y, three rinses in 95% ETOH, three rinses in Xylene substitute and then coverslip. All other tissues appear just fine but the eosinophils just don't show up. Any suggestions are greatly appreciated. Eric A. Breisch, Ph.D. Clinical Anatomist Dept. of Pathology Rady Children's Hospital and Health Center Associate Clinical Professor of Anatomy Dept. of Surgery UCSD School of Medicine _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Tue Feb 5 18:30:17 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Tue Feb 5 18:30:23 2008 Subject: A gentle reminder about using subject line Re: [Histonet] (no subject) References: Message-ID: <000601c86857$72c69700$6601a8c0@Sunney> For those new to or not used to the Histonet messaging, please use the subject line rather ant just hit the reply key. Replying to the daily digest also kicks back all the messages for that day to those who have already received them. The subject is important so others, including me, don't just hit the delete key and not read your valuable inquiries and comment. Thanks Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Margaryan, Naira" To: Sent: Tuesday, February 05, 2008 3:50 PM Subject: [Histonet] (no subject) Dear histo team, I need to stain frozen & FFPE sections for Tuj-1 (AbCam, ab53234) and FFPE sectiond for Netrin-1 (AbCam, ab39370). Any kind of suggestions or even full IHC or IF protocol is appreciated. Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Tue Feb 5 18:35:12 2008 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Feb 5 18:35:15 2008 Subject: [Histonet] eosinophils on frozen section In-Reply-To: <08A0A863637F1349BBFD83A96B27A50A1200DF@uwhis-xchng3.uwhis.hosp.wisc.edu> References: <43B97B4C402C2C44AAA2A8D2C86A88B31A5A78@e2k3backend1.RCHSD.org> <08A0A863637F1349BBFD83A96B27A50A1200DF@uwhis-xchng3.uwhis.hosp.wisc.edu> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD320518E56A@sjhaexc02.sjha.org> Fix in formal-alcohol (no, it is not wearing a tuxedo) 90 ml absolute alcohol - 10 ml 37% formaldehyde... Should work ok then... j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Tuesday, February 05, 2008 7:08 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] eosinophils on frozen section Eric: My guess is that the alcohol is lysing(blowing up) the unfixed eosinophils, therefore they are not there to stain. Fixation stabilizes the cells in order to be stained in regular paraffin sections. Unfortunately, I have done a few trials using different fixatives for fresh tissue to see if I could preserve these little suckers. Nothing really worked satisfactorally. You might try fixing in formalin for a minute or two before you continue on with your stain and see if this helps. (don't forget to wash the sections afterward) Hope this helps. Claire We are experiencing some frustration with our ability to identify eosinophils from frozen sections. For some reason which our frozen section set up does not enable us to identify eosinophils. Eosinophil identification is not impaired when the tissue is submitted for permanents. Could some one please share the reagents used and how the frozen section staining area is set up if they have success identifying eosinophils from a frozen section? We have had many frozen sections done on fresh tissues suspicious for an eosinophilic granuloma but our techniques so far have not rendered consistent eosinophil identification. We presently are using 95% ETOH for fixing the FS slide, quick wash in distilled H2O, 1 minute in Richard Allen Hematoxylin 2, rinse in tap water, 15 seconds in bluing, rinse in water, rinse in 70% ETOH, 1 minute in Richard Allen Eosin Y, three rinses in 95% ETOH, three rinses in Xylene substitute and then coverslip. All other tissues appear just fine but the eosinophils just don't show up. Any suggestions are greatly appreciated. Eric A. Breisch, Ph.D. Clinical Anatomist Dept. of Pathology Rady Children's Hospital and Health Center Associate Clinical Professor of Anatomy Dept. of Surgery UCSD School of Medicine _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From gayle.callis <@t> bresnan.net Tue Feb 5 18:49:57 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Tue Feb 5 18:50:01 2008 Subject: [Histonet] Do mice have tonsils?? References: <47A8F310.20905@umn.edu> Message-ID: <000e01c8685a$321bd7d0$6601a8c0@Sunney> Colleen, Rodents do NOT have tonsils, but have tissues analogous (Sp?) to tonsils, called nasal associated lymphoid tissues located in a rather difficult spot at the back of the nasal turbinates. I suggest you get into the literature and type in murine NALT as there are some excellent articles on morphology and their location. If you want to do frozen sections, you either need to remove these from this area, not easy to do, and takes a lot of practice OR you can do undecalcified bone frozen sections with the Cryojane. The NALT is located above the soft palate, just below the 3rd palatine ridge if you start counting ridges on the soft palate and starting count at the front incisors. These tiny lymphoid tissues are nestled on the roof of the mouth, just below the soft palate, between the back molars. The molars are the complicating factor for doing murine CD markers that only work on frozen sections. I will be happy to send you a powerpoint photograph of a fully decalcified mouse head, mid sagittal section, stained with H&E to show exactly where the NALT is located. I also have a cross section of the murine nasal turbinates showing NALT in that orientation, and much harder to deal with in order to find the NALT. One project had PLP perfused mouse head, immersion fixed longer and then EDTA decalcified, cryoprotected and sectioned on the cryostat to do immunoglobulin staining of turbinate epithelial cells back and into the NALT. We work with NALT a good deal, and I can honestly say that dissection/removal is not easy. However, it can be done but with very gentle, light touch using a pointed, tiny scalpel blade. When we do frozen sections of removed NALT, we can obtain approx 30 - 40 sections, serial, at 5 um and with treated animals to produce inflammed NALT, up to 50 and more serial sections. With undecalcified bone, we get far fewer due to the difficulty of sectioning. G0 into PUBMED, and look for David Pascual, Keri Cscentis on their NALT publication. I believe they put a cartoon of NALT location in that publication. Good luck, Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Colleen Forster" To: Sent: Tuesday, February 05, 2008 4:36 PM Subject: [Histonet] Do mice have tonsils?? > For any of you have done mouse necropsy, do they have tonsils or just > lymph nodes? I have had a request for mouse tonsils.... can't seem to find > them in my searches. I would appreciate any help on this one. > > Thanks, > > Colleen Forster > U of MN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Tue Feb 5 18:58:05 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Tue Feb 5 18:58:08 2008 Subject: [Histonet] eosinophils on frozen section References: <43B97B4C402C2C44AAA2A8D2C86A88B31A5A78@e2k3backend1.RCHSD.org> Message-ID: <001401c8685b$55212f90$6601a8c0@Sunney> We improved our eosinophil staining on frozen sections of murine tissue by switching to NBF immersion fixation, let it sit 10 min, then do the H&E but use eosin/phloxine (Richard Allan stain will work) but stain in eosin/phloxine for 2 minutes. For some reason, the eosin was never taken up as well after a shorter time in eosin alone, and greatly improved with the phloxine added. Sections can be cut a bit thinner too, try 4 um - it should make the granules more apparent against the background and other cells. Also, I suggest adding absolute alcohol after the 95% for better dehydration before clearing. You may have success using alcoholic formalin in order to speed up the fixation a bit. However, when we switched to NBF, we had better staining of eosinophils in frozen sections. If you have the time, you can let NBF fixation go longer, we often let sections sit for days then stain, but with your diagnostic procedure, you will want a bit more speed involved. Good luck Gayle M..Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Breisch, Eric" To: Sent: Tuesday, February 05, 2008 4:32 PM Subject: [Histonet] eosinophils on frozen section To all interested Histonetters: We are experiencing some frustration with our ability to identify eosinophils from frozen sections. For some reason which our frozen section set up does not enable us to identify eosinophils. Eosinophil identification is not impaired when the tissue is submitted for permanents. Could some one please share the reagents used and how the frozen section staining area is set up if they have success identifying eosinophils from a frozen section? We have had many frozen sections done on fresh tissues suspicious for an eosinophilic granuloma but our techniques so far have not rendered consistent eosinophil identification. We presently are using 95% ETOH for fixing the FS slide, quick wash in distilled H2O, 1 minute in Richard Allen Hematoxylin 2, rinse in tap water, 15 seconds in bluing, rinse in water, rinse in 70% ETOH, 1 minute in Richard Allen Eosin Y, three rinses in 95% ETOH, three rinses in Xylene substitute and then coverslip. All other tissues appear just fine but the eosinophils just don't show up. Any suggestions are greatly appreciated. Eric A. Breisch, Ph.D. Clinical Anatomist Dept. of Pathology Rady Children's Hospital and Health Center Associate Clinical Professor of Anatomy Dept. of Surgery UCSD School of Medicine _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jdmd77 <@t> hotmail.com Tue Feb 5 20:06:55 2008 From: jdmd77 <@t> hotmail.com (Julia Dahl) Date: Tue Feb 5 20:07:02 2008 Subject: [Histonet] eosinophils on frozen section In-Reply-To: <43B97B4C402C2C44AAA2A8D2C86A88B31A5A78@e2k3backend1.RCHSD.org> References: <43B97B4C402C2C44AAA2A8D2C86A88B31A5A78@e2k3backend1.RCHSD.org> Message-ID: Agree with Gayle that alcoholic formalin allows for speedier fixation and preservation of eosinophil granule membranes (and therefore staining, rather than degranulation). > Date: Tue, 5 Feb 2008 15:32:31 -0800 > From: ebreisch@rchsd.org > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] eosinophils on frozen section > > To all interested Histonetters: > > > > > > We are experiencing some frustration with our ability to identify > eosinophils from frozen sections. For some reason which our frozen > section set up does not enable us to identify eosinophils. Eosinophil > identification is not impaired when the tissue is submitted for > permanents. Could some one please share the reagents used and how the > frozen section staining area is set up if they have success identifying > eosinophils from a frozen section? We have had many frozen sections done > on fresh tissues suspicious for an eosinophilic granuloma but our > techniques so far have not rendered consistent eosinophil > identification. We presently are using 95% ETOH for fixing the FS slide, > quick wash in distilled H2O, 1 minute in Richard Allen Hematoxylin 2, > rinse in tap water, 15 seconds in bluing, rinse in water, rinse in 70% > ETOH, 1 minute in Richard Allen Eosin Y, three rinses in 95% ETOH, three > rinses in Xylene substitute and then coverslip. All other tissues appear > just fine but the eosinophils just don't show up. Any suggestions are > greatly appreciated. > > > > > > Eric A. Breisch, Ph.D. > > Clinical Anatomist > > Dept. of Pathology > > Rady Children's Hospital and Health Center > > Associate Clinical Professor of Anatomy > > Dept. of Surgery > > UCSD School of Medicine > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Connect and share in new ways with Windows Live. http://www.windowslive.com/share.html?ocid=TXT_TAGHM_Wave2_sharelife_012008 From kwuny <@t> email.cs.nsw.gov.au Tue Feb 5 20:06:29 2008 From: kwuny <@t> email.cs.nsw.gov.au (Young Kwun) Date: Tue Feb 5 20:07:04 2008 Subject: [Histonet] Antibody to Annexin 2 and GAPDH suppliers. Message-ID: <200802061306813.SM00912@csls2816> Dear Histonetters, I am trying to source suppliers of the antibodies to Annexin 2 and Glyceraldehyde-3-phophate dehydrogenase (GAPDH). We are interested in trying on formalin-fixed and paraffin-embedded human tissues. I would be grateful if anybody could suggest me about the information with these antibodies. Thank you in advance. Young Kwun Senior Hospital Scientist Dept. of Anatomical Pathology Concord Hospital Concord NSW 2139 Australia Tel)61-2-9767-6075 Fax)61-2-9767-8427 kwuny@email.cs.nsw.gov.au From sprice2003 <@t> gmail.com Wed Feb 6 05:31:48 2008 From: sprice2003 <@t> gmail.com (Sally Price) Date: Wed Feb 6 05:31:54 2008 Subject: [Histonet] Immunoperoxidase Double staining protocol Message-ID: Reul: An excellent summary of double-staining methods was presented in a paper published last year in HistoLogic - Here's a link to this article: http://www.sakura-americas.com/histologic/pdf/06_dec.pdf Cheers, Sally ------------------------------ Message: 9 Date: Tue, 05 Feb 2008 14:34:27 -0600 From: "Reuel Cornelia" Subject: [Histonet] Immunoperoxidase Double staining protocol To: Message-ID: <47A873F3020000C50002ADF9@nwcl02.tsrh.org> Content-Type: text/plain; charset=US-ASCII I was asked to do an immunoperoxidase double staining on CD61 ,Cd42, pf4 with an unknown mouse antibody that will work on platelets. Can somebody help me or any protocol available that I can refer to. I am used of IF double stianing but not Immunoperoxidase. Thank you. Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 From rcharles <@t> state.pa.us Wed Feb 6 07:09:29 2008 From: rcharles <@t> state.pa.us (Charles, Roger) Date: Wed Feb 6 07:10:16 2008 Subject: [Histonet] Slide printers (ink) Message-ID: <12E4E17FEF6EBE4BAE95BEB3CDCB57000C79D135@enhbgpri04.backup> I would have to say yes since the slides must be stacked on top of each other in the hopper. Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 -----Original Message----- From: pkromund@gundluth.org [mailto:pkromund@gundluth.org] Sent: Tuesday, February 05, 2008 4:35 PM To: Patti Loykasek Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu; Kim Merriam; Charles, Roger Subject: Re: [Histonet] Slide printers (ink) Do any of these slide printers affect precut control tissue on the charged slides? Pamela Patti Loykasek To Sent by: "Charles, Roger" histonet-bounces@ , Kim Merriam lists.utsouthwest ern.edu cc histonet@lists.utsouthwestern.edu Subject 02/05/2008 02:39 Re: [Histonet] Slide printers (ink) PM Roger- We have been using a sakura slide printer for a couple of years. I do agree with your comments about the software - not user friendly at all nor is it intuitive. I have used Access database in the past without these issues. I do not know about the labels, I don't think we've had that issue. Maybe it varies with the label. I don't quite understand your comment on charged slides, as all of the slides we put thru the printer are charged and we have no issues with that. We use charged slides (Okando Plus) from BBC and Probe-On Plus slides from ThermoFisher. What brand of slides were you using & what issue did you have with the slide printer from the usage? Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Hello, > Recently we bought a sakura slide writer that uses ink to print. The > only problem I have found with the ink is if you put labels over the > print and then try to remove the label to verify a number error, or > something like that, the ink will actually come off with the label. If > you can read upside down you can still verify the number. I did run > this ink thru a battery of chemicals including straight formic acid for > 5 minutes and the ink did not leave the slide. I would caution in > buying a Sakura slide write as it has a very unfriendly Access based > program and the writer does not work with charged slides. This has been > verified by me and an independent engineer sent to look at this issue. > I do use a TBS cassette writer and love that so if I can get my sakura > sent back I will be looking at TBS's slide writer too. > Good luck > Roger > > Roger Charles > Microbiologist > Pennsylvania Veterinary Laboratory > 2305 N Cameron St > Harrisburg, PA 17110 > 717-787-8808 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim > Merriam > Sent: Tuesday, February 05, 2008 2:48 PM > To: Histonet > Subject: [Histonet] Slide printers (ink) > > Hi everyone, > > I was wondering what everyone's experience was with the TBS slide > printer (Shurmark-plus), the one that prints with ink? I would > appreciate input, good or bad. > > We currently have an etcher, and we hate it, so we are looking to > trade it in for something that uses ink. I have used the Leica printer > in the past and have had reasonably good luck with it, but I was > wondering about the printer from TBS, I don't know anyone that has one. > > Kim > > > Kim Merriam, MA, HT(ASCP) > Cambridge, MA > > --------------------------------- > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try > it now. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wilson_c <@t> ricerca.com Wed Feb 6 07:17:34 2008 From: wilson_c <@t> ricerca.com (Wilson, Carol) Date: Wed Feb 6 07:17:40 2008 Subject: [Histonet] Cracking of paraffin blocks Message-ID: <9D443EB9D0270143B5AAF190CB1A58A30679622B@dogwood.ricerca.com> Hi All, Recently we have seen an increase in cracks of paraffin blocks when we take them out of the molds. Sometimes it does not affect the tissue, but there are times that the tissue cracks right along with the paraffin. We are using Surgipath infiltration on our processor and Surgipath embedding media in our embedding center. We use reusable metal base molds. Don't know if would matter or not, but we are processing animal tissue. We take temperatures daily of all wax baths.. nothing out of the ordinary. Any suggestions would be greatly appreciated. Thanks, Carol Carol Wilson Team Leader - Histology Ricerca Biosciences, LLC From rjbuesa <@t> yahoo.com Wed Feb 6 08:11:04 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 6 08:11:08 2008 Subject: [Histonet] eosinophils on frozen section In-Reply-To: <43B97B4C402C2C44AAA2A8D2C86A88B31A5A78@e2k3backend1.RCHSD.org> Message-ID: <718450.11225.qm@web61217.mail.yahoo.com> Use a counterstain made of: Eosin Y---0.8 g + phloxine---0.2g in 95% ethanol. Stain 1 minute and dip quickly in distilled water and the RUN (I mean RUNNING) through the alcohols. You can start in 100% EthOL, clear and mount. Ren? J. "Breisch, Eric" wrote: To all interested Histonetters: We are experiencing some frustration with our ability to identify eosinophils from frozen sections. For some reason which our frozen section set up does not enable us to identify eosinophils. Eosinophil identification is not impaired when the tissue is submitted for permanents. Could some one please share the reagents used and how the frozen section staining area is set up if they have success identifying eosinophils from a frozen section? We have had many frozen sections done on fresh tissues suspicious for an eosinophilic granuloma but our techniques so far have not rendered consistent eosinophil identification. We presently are using 95% ETOH for fixing the FS slide, quick wash in distilled H2O, 1 minute in Richard Allen Hematoxylin 2, rinse in tap water, 15 seconds in bluing, rinse in water, rinse in 70% ETOH, 1 minute in Richard Allen Eosin Y, three rinses in 95% ETOH, three rinses in Xylene substitute and then coverslip. All other tissues appear just fine but the eosinophils just don't show up. Any suggestions are greatly appreciated. Eric A. Breisch, Ph.D. Clinical Anatomist Dept. of Pathology Rady Children's Hospital and Health Center Associate Clinical Professor of Anatomy Dept. of Surgery UCSD School of Medicine _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From rjbuesa <@t> yahoo.com Wed Feb 6 08:19:03 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 6 08:19:11 2008 Subject: [Histonet] Cracking of paraffin blocks In-Reply-To: <9D443EB9D0270143B5AAF190CB1A58A30679622B@dogwood.ricerca.com> Message-ID: <790914.78565.qm@web61216.mail.yahoo.com> That type of cracking ussually is caused by an extremely cold "cooling plate" in the embedding center. Have that area at -5?C the most, Ren? J. "Wilson, Carol" wrote: Hi All, Recently we have seen an increase in cracks of paraffin blocks when we take them out of the molds. Sometimes it does not affect the tissue, but there are times that the tissue cracks right along with the paraffin. We are using Surgipath infiltration on our processor and Surgipath embedding media in our embedding center. We use reusable metal base molds. Don't know if would matter or not, but we are processing animal tissue. We take temperatures daily of all wax baths.. nothing out of the ordinary. Any suggestions would be greatly appreciated. Thanks, Carol Carol Wilson Team Leader - Histology Ricerca Biosciences, LLC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From anthony <@t> histotechexchange.com Wed Feb 6 08:10:30 2008 From: anthony <@t> histotechexchange.com (anthony@histotechexchange.com) Date: Wed Feb 6 08:22:17 2008 Subject: Making sure I get this subject line thing correct Re: [Histonet] (no subject) In-Reply-To: <000601c86857$72c69700$6601a8c0@Sunney> References: <000601c86857$72c69700$6601a8c0@Sunney> Message-ID: <1984.65.40.219.240.1202307030.squirrel@host7.wfdns.com> Dear Gayle: I have used the "Reply" button on my computer to send this email. Is this causing the problem? Or are you asking me to put some new info in my subject line to delineate the new path I am taking the conversation? Either way sorry for any incontinence caused in using the histonet, it is a fantastic venue for discussion. Yours truly, Anthony Williams HT (ASCP) Histotech Exchange LLC 19 Whitmore Street Lexington, VA 24450 T 1 877 464 8911 F 1 540 301 0071 anthony@histotechexchange.com www.histotechexchange.com For those new to or not used to the Histonet messaging, please use the > subject line rather ant just hit the reply key. Replying to the daily > digest also kicks back all the messages for that day to those who have > already received them. The subject is important so others, including me, > don't just hit the delete key and not read your valuable inquiries and > comment. > > Thanks > Gayle M. Callis > HTL/HT/MT(ASCP) > Bozeman MT 59715 > > > ----- Original Message ----- > From: "Margaryan, Naira" > To: > Sent: Tuesday, February 05, 2008 3:50 PM > Subject: [Histonet] (no subject) > > > Dear histo team, > > > > I need to stain frozen & FFPE sections for Tuj-1 (AbCam, ab53234) and > FFPE sectiond for Netrin-1 (AbCam, ab39370). Any kind of suggestions or > even full IHC or IF protocol is appreciated. > > > > Thanks in advance, > > Naira > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mareike <@t> hi.is Wed Feb 6 08:43:39 2008 From: mareike <@t> hi.is (Mareike Heimann) Date: Wed Feb 6 08:43:35 2008 Subject: [Histonet] Basic Fuchsin vs. Pararosaniline Message-ID: <00a601c868ce$a9fcd550$5a96d082@MrDarcy> Dear all, I would like to try using the Leder stain for mast cells. Before I buy pararosaniline, which is not available in our lab, I wanted to ask if it is possible to use Basic Fuchsin (which we do have) instead? Thanks in advance for your advice, Mareike Heimann University of Iceland From Terry.Marshall <@t> rothgen.nhs.uk Wed Feb 6 08:47:09 2008 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Wed Feb 6 08:47:04 2008 Subject: A gentle reminder about using subject line Re: [Histonet] (no subject) Message-ID: <5C0BED61F529364E86309CADEA63FEF2F3AFD0@TRFT-EX01.xRothGen.nhs.uk> Gayle, I've never known even a slight tendency to obscurity in your posts, but I'm blowed if I know what you are getting at in this case:-) Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: 06 February 2008 00:30 To: Margaryan, Naira; histonet@lists.utsouthwestern.edu Subject: A gentle reminder about using subject line Re: [Histonet] (no subject) For those new to or not used to the Histonet messaging, please use the subject line rather ant just hit the reply key. Replying to the daily digest also kicks back all the messages for that day to those who have already received them. The subject is important so others, including me, don't just hit the delete key and not read your valuable inquiries and comment. Thanks Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Margaryan, Naira" To: Sent: Tuesday, February 05, 2008 3:50 PM Subject: [Histonet] (no subject) Dear histo team, I need to stain frozen & FFPE sections for Tuj-1 (AbCam, ab53234) and FFPE sectiond for Netrin-1 (AbCam, ab39370). Any kind of suggestions or even full IHC or IF protocol is appreciated. Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ###################################################################### This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. ###################################################################### From mcauliff <@t> umdnj.edu Wed Feb 6 09:05:14 2008 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Feb 6 09:05:29 2008 Subject: [Histonet] Basic Fuchsin vs. Pararosaniline In-Reply-To: <00a601c868ce$a9fcd550$5a96d082@MrDarcy> References: <00a601c868ce$a9fcd550$5a96d082@MrDarcy> Message-ID: <47A9CCAA.5090204@umdnj.edu> Hi Mareike: It depends on how much pararosanaline is in your basic fuchsin. According to John Kiernan modern samples of BF are almost entirely pararosanaline or rosanaline. Older samples may also contain new fuchsin and/or magenta II. If the dye is certified by the Biological Stain Commission you could contact them and find out what is in your batch. If it is not certified all you can do it try it or buy a new supply. Again we see the advantage of using certified dyes. Geoff Mareike Heimann wrote: > Dear all, > > I would like to try using the Leder stain for mast cells. Before I buy pararosaniline, which is not available in our lab, I wanted to ask if it is possible to use Basic Fuchsin (which we do have) instead? > > Thanks in advance for your advice, > Mareike Heimann > University of Iceland > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From CIngles <@t> uwhealth.org Wed Feb 6 09:05:50 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Wed Feb 6 09:06:01 2008 Subject: [Histonet] OT: the weather Message-ID: <08A0A863637F1349BBFD83A96B27A50A1200E1@uwhis-xchng3.uwhis.hosp.wisc.edu> Hey guys: I don't know about you, but I'm really beginning to wonder about the weather. Anyway- everyone in the southern states, especially Arkansas, still there? I gripe about the snow this year, but geez. How bad was the damage from the tornadoes down in that area. Just thinking of everyone, and hoping all is well with everyone. Stay warm/dry/unburnt/etc. You bunch from across the pond can send us your extra show shovels and inflatable rafts. Anywhere you could put us up if it gets too bad over here? :) Claire From NMargaryan <@t> childrensmemorial.org Wed Feb 6 09:19:05 2008 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Wed Feb 6 09:19:38 2008 Subject: [Histonet] Tuj-1 and Netrin-1 Message-ID: Dear histo team, I am sorry, I forgot to mention a subject of my questions and I fortunately I have to repeat my question. I need to stain frozen & FFPE sections for Tuj-1 (AbCam, ab53234) and FFPE sectiond for Netrin-1 (AbCam, ab39370). What is the positive control tissue for these both Abs except the brain? Any kind of suggestions or even full IHC or IF protocol is appreciated. Thanks in advance, Naira From lhunt <@t> uta.edu Wed Feb 6 09:20:17 2008 From: lhunt <@t> uta.edu (Laura Hunt) Date: Wed Feb 6 09:20:27 2008 Subject: [Histonet] Staining coral tissues embedded in resin In-Reply-To: References: Message-ID: <5DDB1B77-09A7-4C49-842C-711D3344F608@uta.edu> Hi everyone, I am new to the forum. I am working on coral tissues embedded in Immunobed resin and paraffin. We would like to stain both with H&E and Azure II /Basic Fuchsin. I have never tried staining plastic with H&E. Any suggestions or comments would be great! Since corals have a calcium carbonate skeleton, the tissues were decalcified in a EDTA solution. Laura Hunt UTA On Feb 5, 2008, at 8:07 PM, histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. EpoR antibody (Colleen Forster) > 2. RE: sections falling off (Debrosse-Serra, Beatrice) > 3. Re: sections falling off (Rene J Buesa) > 4. Re: Alcian Blue Quality Control OT (Robert Richmond) > 5. HP staining (Martin, Gary) > 6. Slide printers (ink) (Kim Merriam) > 7. RE: Slide printers (ink) (Charles, Roger) > 8. RE: Slide printers (ink) (Weems, Joyce) > 9. Immunoperoxidase Double staining protocol (Reuel Cornelia) > 10. Re: Slide printers (ink) (Patti Loykasek) > 11. RE: Storage codes (Christine Tambasco) > 12. Re: Slide printers (ink) (pkromund@gundluth.org) > 13. Re: Immunoperoxidase Double staining protocol (Rene J Buesa) > 14. thanks for the support and good wishes (Sebree Linda A.) > 15. (no subject) (Margaryan, Naira) > 16. Do mice have tonsils?? (Colleen Forster) > 17. eosinophils on frozen section (Breisch, Eric) > 18. RE: eosinophils on frozen section (Ingles Claire) > 19. A gentle reminder about using subject line Re: [Histonet] (no > subject) (Gayle Callis) > 20. RE: eosinophils on frozen section (Weems, Joyce) > 21. Re: Do mice have tonsils?? (Gayle Callis) > 22. Re: eosinophils on frozen section (Gayle Callis) > 23. RE: eosinophils on frozen section (Julia Dahl) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 05 Feb 2008 12:20:37 -0600 > From: Colleen Forster > Subject: [Histonet] EpoR antibody > To: histonet@lists.utsouthwestern.edu > Message-ID: <47A8A8F5.3090606@umn.edu> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Hello histonetters, > > Is anyone doing the EpoR antibody on FFPE for IHC? If so, could you > share the vendor and a starting dilution.....thanks. > > Colleen Forster > U of MN > > > > ------------------------------ > > Message: 2 > Date: Tue, 5 Feb 2008 10:30:10 -0800 > From: "Debrosse-Serra, Beatrice" > Subject: RE: [Histonet] sections falling off > To: "Olek Michalski" , "Histonet" > > Message-ID: > > <8404DFBED5207B4B8E5EEF4332CEEA5305BD4C99@lajamrexm01.amer.pfizer.com> > Content-Type: text/plain; charset="us-ascii" > > Try Newcomers silane coated slides. They are not cheap, but the > sections > stay on very well. > > Beatrice DeBrosse-Serra > Pathology Scientist > Pfizer Global Research & Development > CB4, 2150 > 10646 Science Center Drive > San Diego, CA 92121 > Phone# 858-622-5986 > Fax# 858-678-8290 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Olek > Michalski > Sent: Tuesday, February 05, 2008 10:00 AM > To: Histonet > Subject: [Histonet] sections falling off > > > Dear Histonetters, > > a friend of mine just faced a massive sections falling off. She is > doing > > Nissl staining in mouse brain (brains perfused with formalin, > postfixed > 3 > days, and cryosectioned) on poly-L-lysined slides. She just managed to > pass trough xylene and decreasing grades of alcohol to water and the > sections started to detach. We were trying to reattach them but it > went > > out that sections are not flat any more. It seems like the tissue is > rehydrated unevenly, but keeping it in water for hours didn't work. Is > there any way to spread these sections not damaging them at the same > time? > She is likely to rescue this sections even if some work is needed. > > Best regards > Olek Michalski > -- > Laboratory of Neurobiology > of Development and Evolution > > Nencki Institute of Experimental Biology > ul. Pasteura 3, 02-093 Warszawa, Poland > Tel. +48 22 5892268, Fax +48 22 8225342 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 3 > Date: Tue, 5 Feb 2008 10:30:54 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] sections falling off > To: Olek Michalski , Histonet > > Message-ID: <87465.99172.qm@web61214.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > If they were cryosectioned she did not have to go through xylene or > graded alcohols to "dewax and hydrate" since there was no > dehydration or wax infiltration to begin with. > > Cryosectioned sections just need to wash away the medium used to > cryosection (OCT perhaps?) and just stain with the aqueous solutions. > > Later they can de dehydrated and cleared if a permanent mount is > desired. > Try to float the sections in a water bath and pick them up and > don't try again to use xylene or alcohols. > Probably she was following a procedure for paraffin embedded tissue > that should have been adapted to frozen sections and it was not. > Ren? J. > > Olek Michalski wrote: > > Dear Histonetters, > > a friend of mine just faced a massive sections falling off. She is > doing > Nissl staining in mouse brain (brains perfused with formalin, > postfixed 3 > days, and cryosectioned) on poly-L-lysined slides. She just managed to > pass trough xylene and decreasing grades of alcohol to water and the > sections started to detach. We were trying to reattach them but it > went > out that sections are not flat any more. It seems like the tissue is > rehydrated unevenly, but keeping it in water for hours didn't work. Is > there any way to spread these sections not damaging them at the same > time? > She is likely to rescue this sections even if some work is needed. > > Best regards > Olek Michalski > -- > Laboratory of Neurobiology > of Development and Evolution > > Nencki Institute of Experimental Biology > ul. Pasteura 3, 02-093 Warszawa, Poland > Tel. +48 22 5892268, Fax +48 22 8225342 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Looking for last minute shopping deals? Find them fast with Yahoo! > Search. > > ------------------------------ > > Message: 4 > Date: Tue, 5 Feb 2008 13:45:34 -0500 > From: "Robert Richmond" > Subject: [Histonet] Re: Alcian Blue Quality Control OT > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Claire Ingles remarks: So that's what GOP means! > > Not this grumpy old pathologist - I voted for Barack Obama! > > Bob Richmond > Samurai Pathologist > Knoxville TN > > > > ------------------------------ > > Message: 5 > Date: Tue, 5 Feb 2008 11:00:57 -0800 > From: "Martin, Gary" > Subject: [Histonet] HP staining > To: > Message-ID: > <6ED9D4252F278841A0593D3D788AF24C01ACDF72@mailsvr.MARSHMED.local> > Content-Type: text/plain; charset="us-ascii" > > To all who responded to my e-mail concerning rapid HP staining to > include a yellow back ground ... thank you very much ... my question > was > answered. > > Gray > > > > ------------------------------ > > Message: 6 > Date: Tue, 5 Feb 2008 11:48:08 -0800 (PST) > From: Kim Merriam > Subject: [Histonet] Slide printers (ink) > To: Histonet > Message-ID: <813260.30617.qm@web50306.mail.re2.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi everyone, > > I was wondering what everyone's experience was with the TBS slide > printer (Shurmark-plus), the one that prints with ink? I would > appreciate input, good or bad. > > We currently have an etcher, and we hate it, so we are looking to > trade it in for something that uses ink. I have used the Leica > printer in the past and have had reasonably good luck with it, but I > was wondering about the printer from TBS, I don't know anyone that > has one. > > Kim > > > Kim Merriam, MA, HT(ASCP) > Cambridge, MA > > --------------------------------- > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. > Try it now. > > ------------------------------ > > Message: 7 > Date: Tue, 5 Feb 2008 14:58:42 -0500 > From: "Charles, Roger" > Subject: RE: [Histonet] Slide printers (ink) > To: "Kim Merriam" > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > <12E4E17FEF6EBE4BAE95BEB3CDCB57000943BB6E@enhbgpri04.backup> > Content-Type: text/plain; charset="US-ASCII" > > Hello, > Recently we bought a sakura slide writer that uses ink to print. The > only problem I have found with the ink is if you put labels over the > print and then try to remove the label to verify a number error, or > something like that, the ink will actually come off with the label. > If > you can read upside down you can still verify the number. I did run > this ink thru a battery of chemicals including straight formic acid > for > 5 minutes and the ink did not leave the slide. I would caution in > buying a Sakura slide write as it has a very unfriendly Access based > program and the writer does not work with charged slides. This has > been > verified by me and an independent engineer sent to look at this issue. > I do use a TBS cassette writer and love that so if I can get my sakura > sent back I will be looking at TBS's slide writer too. > Good luck > Roger > > Roger Charles > Microbiologist > Pennsylvania Veterinary Laboratory > 2305 N Cameron St > Harrisburg, PA 17110 > 717-787-8808 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim > Merriam > Sent: Tuesday, February 05, 2008 2:48 PM > To: Histonet > Subject: [Histonet] Slide printers (ink) > > Hi everyone, > > I was wondering what everyone's experience was with the TBS slide > printer (Shurmark-plus), the one that prints with ink? I would > appreciate input, good or bad. > > We currently have an etcher, and we hate it, so we are looking to > trade it in for something that uses ink. I have used the Leica > printer > in the past and have had reasonably good luck with it, but I was > wondering about the printer from TBS, I don't know anyone that has > one. > > Kim > > > Kim Merriam, MA, HT(ASCP) > Cambridge, MA > > --------------------------------- > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. > Try > it now. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 8 > Date: Tue, 5 Feb 2008 15:25:58 -0500 > From: "Weems, Joyce" > Subject: RE: [Histonet] Slide printers (ink) > To: "Charles, Roger" , "Kim Merriam" > > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > <1CD6831EB9B26D45B0A3EAA79F7EBD320518E54D@sjhaexc02.sjha.org> > Content-Type: text/plain; charset="us-ascii" > > We have Sakura cassette and slide writers. We print charged slides > daily. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Charles, > Roger > Sent: Tuesday, February 05, 2008 2:59 PM > To: Kim Merriam > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Slide printers (ink) > > Hello, > Recently we bought a sakura slide writer that uses ink to print. The > only problem I have found with the ink is if you put labels over the > print and then try to remove the label to verify a number error, or > something like that, the ink will actually come off with the label. > If > you can read upside down you can still verify the number. I did run > this ink thru a battery of chemicals including straight formic acid > for > 5 minutes and the ink did not leave the slide. I would caution in > buying a Sakura slide write as it has a very unfriendly Access based > program and the writer does not work with charged slides. This has > been > verified by me and an independent engineer sent to look at this issue. > I do use a TBS cassette writer and love that so if I can get my sakura > sent back I will be looking at TBS's slide writer too. > Good luck > Roger > > Roger Charles > Microbiologist > Pennsylvania Veterinary Laboratory > 2305 N Cameron St > Harrisburg, PA 17110 > 717-787-8808 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim > Merriam > Sent: Tuesday, February 05, 2008 2:48 PM > To: Histonet > Subject: [Histonet] Slide printers (ink) > > Hi everyone, > > I was wondering what everyone's experience was with the TBS slide > printer (Shurmark-plus), the one that prints with ink? I would > appreciate input, good or bad. > > We currently have an etcher, and we hate it, so we are looking to > trade it in for something that uses ink. I have used the Leica > printer > in the past and have had reasonably good luck with it, but I was > wondering about the printer from TBS, I don't know anyone that has > one. > > Kim > > > Kim Merriam, MA, HT(ASCP) > Cambridge, MA > > --------------------------------- > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. > Try > it now. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice ** The information contained in this message > may be privileged and is confidential information intended for the > use of the addressee listed above. If you are neither the intended > recipient nor the employee or agent responsible for delivering this > message to the intended recipient, you are hereby notified that any > disclosure, copying, distribution or the taking of any action in > reliance on the contents of this information is strictly prohibited. > If you have received this communication in error, please notify us > immediately by replying to the message and deleting it from your > computer. Thank you. Saint Joseph's Health System, Inc. > > > > ------------------------------ > > Message: 9 > Date: Tue, 05 Feb 2008 14:34:27 -0600 > From: "Reuel Cornelia" > Subject: [Histonet] Immunoperoxidase Double staining protocol > To: > Message-ID: <47A873F3020000C50002ADF9@nwcl02.tsrh.org> > Content-Type: text/plain; charset=US-ASCII > > I was asked to do an immunoperoxidase double staining on CD61 ,Cd42, > pf4 with an unknown mouse antibody that will work on platelets. Can > somebody help me or any protocol available that I can refer to. I am > used of IF double stianing but not Immunoperoxidase. Thank you. > > Reuel Cornelia, BS MT, AMT > Cellular Pathology > Texas Scottish Rite Hospital for Children > 2222 Welborn Street > Dallas, TX 75219 > Tel: 214-559-7766 > fax: 214-559-7768 > > ******************************************************************************************************************* > Texas Scottish Rite Hospital for Children is one of the nation's > leading pediatric centers for the > treatment of orthopedic conditions, certain related neurological > disorders and learning disorders, > such as dyslexia. This email transmission and/or its attachments > may contain confidential health > information, intended only for the use of the individual or entity > named above. > > The authorized recipient of this information is prohibited from > disclosing it to any other party unless > required to do so by law and is required to delete/destroy the > information after its stated need has > been fulfilled. If you are not the intended recipient, any > disclosure, copying, distribution or action > taken in reliance on the contents of this email transmission is > prohibited. If you have received this > information in error, please notify the sender immediately and > delete this information. > > We appreciate your efforts to protect the children's confidential > information. > ******************************************************************************************************************* > > > > > > ------------------------------ > > Message: 10 > Date: Tue, 05 Feb 2008 12:39:50 -0800 > From: Patti Loykasek > Subject: Re: [Histonet] Slide printers (ink) > To: "Charles, Roger" , Kim Merriam > > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset="US-ASCII" > > Roger- We have been using a sakura slide printer for a couple of > years. I do > agree with your comments about the software - not user friendly at > all nor > is it intuitive. I have used Access database in the past without these > issues. I do not know about the labels, I don't think we've had that > issue. > Maybe it varies with the label. I don't quite understand your > comment on > charged slides, as all of the slides we put thru the printer are > charged and > we have no issues with that. We use charged slides (Okando Plus) > from BBC > and Probe-On Plus slides from ThermoFisher. What brand of slides > were you > using & what issue did you have with the slide printer from the usage? > > > Patti Loykasek BS, HTL, QIHC > PhenoPath Laboratories > Seattle, WA > > > > > > > >> Hello, >> Recently we bought a sakura slide writer that uses ink to print. The >> only problem I have found with the ink is if you put labels over the >> print and then try to remove the label to verify a number error, or >> something like that, the ink will actually come off with the >> label. If >> you can read upside down you can still verify the number. I did run >> this ink thru a battery of chemicals including straight formic acid >> for >> 5 minutes and the ink did not leave the slide. I would caution in >> buying a Sakura slide write as it has a very unfriendly Access based >> program and the writer does not work with charged slides. This has >> been >> verified by me and an independent engineer sent to look at this >> issue. >> I do use a TBS cassette writer and love that so if I can get my >> sakura >> sent back I will be looking at TBS's slide writer too. >> Good luck >> Roger >> >> Roger Charles >> Microbiologist >> Pennsylvania Veterinary Laboratory >> 2305 N Cameron St >> Harrisburg, PA 17110 >> 717-787-8808 >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim >> Merriam >> Sent: Tuesday, February 05, 2008 2:48 PM >> To: Histonet >> Subject: [Histonet] Slide printers (ink) >> >> Hi everyone, >> >> I was wondering what everyone's experience was with the TBS slide >> printer (Shurmark-plus), the one that prints with ink? I would >> appreciate input, good or bad. >> >> We currently have an etcher, and we hate it, so we are looking to >> trade it in for something that uses ink. I have used the Leica >> printer >> in the past and have had reasonably good luck with it, but I was >> wondering about the printer from TBS, I don't know anyone that has >> one. >> >> Kim >> >> >> Kim Merriam, MA, HT(ASCP) >> Cambridge, MA >> >> --------------------------------- >> Be a better friend, newshound, and know-it-all with Yahoo! Mobile. >> Try >> it now. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > This e-mail message, including any attachments, is for the sole use > of the > intended recipients and may contain privileged information. Any > unauthorized > review, use, disclosure or distribution is prohibited. If you are > not the intended > recipient, please contact the sender by e-mail and destroy all > copies of the > original message, or you may call PhenoPath Laboratories, Seattle, > WA U.S.A. > at (206) 374-9000. > > > > > ------------------------------ > > Message: 11 > Date: Tue, 5 Feb 2008 21:10:37 +0000 > From: Christine Tambasco > Subject: RE: [Histonet] Storage codes > To: Barbara Albert , histonet > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > I always kept my gold chloride in the refrigerator. Yours is in a > vial though so is it a powder? > That is what I used to have I kept at room temperature. > Just my advice. > Christine Tambasco, HT (ASCP) > St. Marys Hospital > Amsterdam, NY> Date: Mon, 4 Feb 2008 15:33:30 -0800> From: barbaraaalbert@yahoo.com > > To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] Storage > codes> > Hi all,> I just received some vials of Gold Chloride that I > had ordered and was checking the label for stoarge requirements. It > has "Storage Code White". I haven't heard of storage codes and want > to know where I can go to find out what they mean.> > Thanks,> > Barbara Albert> UCSF Medical Center> San Francisco> > > > ---------------------------------> Never miss a thing. Make Yahoo > your homepage.> _______________________________________________> > Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _________________________________________________________________ > Climb to the top of the charts! Play the word scramble challenge > with star power. > http://club.live.com/star_shuffle.aspx?icid=starshuffle_wlmailtextlink_jan > > ------------------------------ > > Message: 12 > Date: Tue, 5 Feb 2008 15:34:42 -0600 > From: pkromund@gundluth.org > Subject: Re: [Histonet] Slide printers (ink) > To: Patti Loykasek > Cc: histonet@lists.utsouthwestern.edu, > histonet-bounces@lists.utsouthwestern.edu, "Charles, Roger" > > Message-ID: > > > > Content-Type: text/plain; charset=US-ASCII > > Do any of these slide printers affect precut control tissue on the > charged > slides? > Pamela > > > > Patti Loykasek > > ath.com> To > Sent by: "Charles, Roger" > histonet-bounces@ , Kim > Merriam > lists.utsouthwest > > ern.edu cc > > histonet@lists.utsouthwestern.edu > > Subject > 02/05/2008 02:39 Re: [Histonet] Slide printers > (ink) > PM > > > > > > > > > > Roger- We have been using a sakura slide printer for a couple of > years. I > do > agree with your comments about the software - not user friendly at > all nor > is it intuitive. I have used Access database in the past without these > issues. I do not know about the labels, I don't think we've had that > issue. > Maybe it varies with the label. I don't quite understand your > comment on > charged slides, as all of the slides we put thru the printer are > charged > and > we have no issues with that. We use charged slides (Okando Plus) > from BBC > and Probe-On Plus slides from ThermoFisher. What brand of slides > were you > using & what issue did you have with the slide printer from the usage? > > > Patti Loykasek BS, HTL, QIHC > PhenoPath Laboratories > Seattle, WA > > > > > > > >> Hello, >> Recently we bought a sakura slide writer that uses ink to print. The >> only problem I have found with the ink is if you put labels over the >> print and then try to remove the label to verify a number error, or >> something like that, the ink will actually come off with the >> label. If >> you can read upside down you can still verify the number. I did run >> this ink thru a battery of chemicals including straight formic acid >> for >> 5 minutes and the ink did not leave the slide. I would caution in >> buying a Sakura slide write as it has a very unfriendly Access based >> program and the writer does not work with charged slides. This has >> been >> verified by me and an independent engineer sent to look at this >> issue. >> I do use a TBS cassette writer and love that so if I can get my >> sakura >> sent back I will be looking at TBS's slide writer too. >> Good luck >> Roger >> >> Roger Charles >> Microbiologist >> Pennsylvania Veterinary Laboratory >> 2305 N Cameron St >> Harrisburg, PA 17110 >> 717-787-8808 >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim >> Merriam >> Sent: Tuesday, February 05, 2008 2:48 PM >> To: Histonet >> Subject: [Histonet] Slide printers (ink) >> >> Hi everyone, >> >> I was wondering what everyone's experience was with the TBS slide >> printer (Shurmark-plus), the one that prints with ink? I would >> appreciate input, good or bad. >> >> We currently have an etcher, and we hate it, so we are looking to >> trade it in for something that uses ink. I have used the Leica >> printer >> in the past and have had reasonably good luck with it, but I was >> wondering about the printer from TBS, I don't know anyone that has >> one. >> >> Kim >> >> >> Kim Merriam, MA, HT(ASCP) >> Cambridge, MA >> >> --------------------------------- >> Be a better friend, newshound, and know-it-all with Yahoo! Mobile. >> Try >> it now. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > This e-mail message, including any attachments, is for the sole use > of the > intended recipients and may contain privileged information. Any > unauthorized > review, use, disclosure or distribution is prohibited. If you are > not the > intended > recipient, please contact the sender by e-mail and destroy all > copies of > the > original message, or you may call PhenoPath Laboratories, Seattle, WA > U.S.A. > at (206) 374-9000. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 13 > Date: Tue, 5 Feb 2008 13:46:41 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] Immunoperoxidase Double staining protocol > To: Reuel Cornelia , > histonet@lists.utsouthwestern.edu > Message-ID: <784337.61146.qm@web61225.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Generally speaking you will have to run the whole protocol for one > of the Abs and use one chromogen (of your selection). After that, > since you already did HIER and blocked the internal peroxidase, you > will treat the section with the second Ab, will link/detect it and > use another chromogen that ideally should have a different color. > The first chromogen could be regular DAB and for the second you can > use also DAB but with incorporated nickel that will give a deep/blue > purple color, in contrast with the brown/reddish normal color of the > DAB. > The thing is that you don't need any special protocol, just your > regular IHC protocol for FFPE tissue, run twice with two different > chromogens. > Ren? J. > > Reuel Cornelia wrote: > I was asked to do an immunoperoxidase double staining on > CD61 ,Cd42, pf4 with an unknown mouse antibody that will work on > platelets. Can somebody help me or any protocol available that I can > refer to. I am used of IF double stianing but not Immunoperoxidase. > Thank you. > > Reuel Cornelia, BS MT, AMT > Cellular Pathology > Texas Scottish Rite Hospital for Children > 2222 Welborn Street > Dallas, TX 75219 > Tel: 214-559-7766 > fax: 214-559-7768 > > ******************************************************************************************************************* > Texas Scottish Rite Hospital for Children is one of the nation's > leading pediatric centers for the > treatment of orthopedic conditions, certain related neurological > disorders and learning disorders, > such as dyslexia. This email transmission and/or its attachments may > contain confidential health > information, intended only for the use of the individual or entity > named above. > > The authorized recipient of this information is prohibited from > disclosing it to any other party unless > required to do so by law and is required to delete/destroy the > information after its stated need has > been fulfilled. If you are not the intended recipient, any > disclosure, copying, distribution or action > taken in reliance on the contents of this email transmission is > prohibited. If you have received this > information in error, please notify the sender immediately and > delete this information. > > We appreciate your efforts to protect the children's confidential > information. > ******************************************************************************************************************* > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. > Try it now. > > ------------------------------ > > Message: 14 > Date: Tue, 5 Feb 2008 15:47:03 -0600 > From: "Sebree Linda A." > Subject: [Histonet] thanks for the support and good wishes > To: "Histonet" > Message-ID: > > > > Content-Type: text/plain; charset="US-ASCII" > > Hi all, > > Well apparently our CAP inspector took the word of our supervisor on > everything pertaining to our lab, reviewed some of our slides and was > out the door before the weather associated with the "winter storm > warning" arrived! Some times its good to live in the land of ice and > snow! > > Thanks for all the good luck wishes! > > Linda Sebree, HT(ASCP) > University of Wisconsin Hospital & Clinics > IHC/ISH Laboratory > A4/204-3224 > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > FAX: (608)262-7174 > > > > ------------------------------ > > Message: 15 > Date: Tue, 5 Feb 2008 16:50:57 -0600 > From: "Margaryan, Naira" > Subject: [Histonet] (no subject) > To: > Message-ID: > > > > Content-Type: text/plain; charset="us-ascii" > > Dear histo team, > > > > I need to stain frozen & FFPE sections for Tuj-1 (AbCam, ab53234) and > FFPE sectiond for Netrin-1 (AbCam, ab39370). Any kind of suggestions > or > even full IHC or IF protocol is appreciated. > > > > Thanks in advance, > > Naira > > > > > > > > > > ------------------------------ > > Message: 16 > Date: Tue, 05 Feb 2008 17:36:48 -0600 > From: Colleen Forster > Subject: [Histonet] Do mice have tonsils?? > To: histonet@lists.utsouthwestern.edu > Message-ID: <47A8F310.20905@umn.edu> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > For any of you have done mouse necropsy, do they have tonsils or just > lymph nodes? I have had a request for mouse tonsils.... can't seem to > find them in my searches. I would appreciate any help on this one. > > Thanks, > > Colleen Forster > U of MN > > > > ------------------------------ > > Message: 17 > Date: Tue, 5 Feb 2008 15:32:31 -0800 > From: "Breisch, Eric" > Subject: [Histonet] eosinophils on frozen section > To: > Message-ID: > <43B97B4C402C2C44AAA2A8D2C86A88B31A5A78@e2k3backend1.RCHSD.org> > Content-Type: text/plain; charset="US-ASCII" > > To all interested Histonetters: > > > > > > We are experiencing some frustration with our ability to identify > eosinophils from frozen sections. For some reason which our frozen > section set up does not enable us to identify eosinophils. Eosinophil > identification is not impaired when the tissue is submitted for > permanents. Could some one please share the reagents used and how the > frozen section staining area is set up if they have success > identifying > eosinophils from a frozen section? We have had many frozen sections > done > on fresh tissues suspicious for an eosinophilic granuloma but our > techniques so far have not rendered consistent eosinophil > identification. We presently are using 95% ETOH for fixing the FS > slide, > quick wash in distilled H2O, 1 minute in Richard Allen Hematoxylin 2, > rinse in tap water, 15 seconds in bluing, rinse in water, rinse in 70% > ETOH, 1 minute in Richard Allen Eosin Y, three rinses in 95% ETOH, > three > rinses in Xylene substitute and then coverslip. All other tissues > appear > just fine but the eosinophils just don't show up. Any suggestions are > greatly appreciated. > > > > > > Eric A. Breisch, Ph.D. > > Clinical Anatomist > > Dept. of Pathology > > Rady Children's Hospital and Health Center > > Associate Clinical Professor of Anatomy > > Dept. of Surgery > > UCSD School of Medicine > > > > > > ------------------------------ > > Message: 18 > Date: Tue, 5 Feb 2008 18:08:15 -0600 > From: "Ingles Claire" > Subject: RE: [Histonet] eosinophils on frozen section > To: > Message-ID: > <08A0A863637F1349BBFD83A96B27A50A1200DF@uwhis-xchng3.uwhis.hosp.wisc.edu > > > > Content-Type: text/plain; charset="iso-8859-1" > > Eric: > My guess is that the alcohol is lysing(blowing up) the unfixed > eosinophils, therefore they are not there to stain. Fixation > stabilizes the cells in order to be stained in regular paraffin > sections. Unfortunately, I have done a few trials using different > fixatives for fresh tissue to see if I could preserve these little > suckers. Nothing really worked satisfactorally. You might try fixing > in formalin for a minute or two before you continue on with your > stain and see if this helps. (don't forget to wash the sections > afterward) Hope this helps. > Claire > > > > We are experiencing some frustration with our ability to identify > eosinophils from frozen sections. For some reason which our frozen > section set up does not enable us to identify eosinophils. Eosinophil > identification is not impaired when the tissue is submitted for > permanents. Could some one please share the reagents used and how the > frozen section staining area is set up if they have success > identifying > eosinophils from a frozen section? We have had many frozen sections > done > on fresh tissues suspicious for an eosinophilic granuloma but our > techniques so far have not rendered consistent eosinophil > identification. We presently are using 95% ETOH for fixing the FS > slide, > quick wash in distilled H2O, 1 minute in Richard Allen Hematoxylin 2, > rinse in tap water, 15 seconds in bluing, rinse in water, rinse in 70% > ETOH, 1 minute in Richard Allen Eosin Y, three rinses in 95% ETOH, > three > rinses in Xylene substitute and then coverslip. All other tissues > appear > just fine but the eosinophils just don't show up. Any suggestions are > greatly appreciated. > > > > > > Eric A. Breisch, Ph.D. > > Clinical Anatomist > > Dept. of Pathology > > Rady Children's Hospital and Health Center > > Associate Clinical Professor of Anatomy > > Dept. of Surgery > > UCSD School of Medicine > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 19 > Date: Tue, 5 Feb 2008 17:30:17 -0700 > From: "Gayle Callis" > Subject: A gentle reminder about using subject line Re: [Histonet] (no > subject) > To: "Margaryan, Naira" , > > Message-ID: <000601c86857$72c69700$6601a8c0@Sunney> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > For those new to or not used to the Histonet messaging, please use the > subject line rather ant just hit the reply key. Replying to the daily > digest also kicks back all the messages for that day to those who have > already received them. The subject is important so others, including > me, > don't just hit the delete key and not read your valuable inquiries and > comment. > > Thanks > Gayle M. Callis > HTL/HT/MT(ASCP) > Bozeman MT 59715 > > > ----- Original Message ----- > From: "Margaryan, Naira" > To: > Sent: Tuesday, February 05, 2008 3:50 PM > Subject: [Histonet] (no subject) > > > Dear histo team, > > > > I need to stain frozen & FFPE sections for Tuj-1 (AbCam, ab53234) and > FFPE sectiond for Netrin-1 (AbCam, ab39370). Any kind of suggestions > or > even full IHC or IF protocol is appreciated. > > > > Thanks in advance, > > Naira > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 20 > Date: Tue, 5 Feb 2008 19:35:12 -0500 > From: "Weems, Joyce" > Subject: RE: [Histonet] eosinophils on frozen section > To: "Ingles Claire" , > > Message-ID: > <1CD6831EB9B26D45B0A3EAA79F7EBD320518E56A@sjhaexc02.sjha.org> > Content-Type: text/plain; charset="us-ascii" > > Fix in formal-alcohol (no, it is not wearing a tuxedo) 90 ml absolute > alcohol - 10 ml 37% formaldehyde... Should work ok then... j > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles > Claire > Sent: Tuesday, February 05, 2008 7:08 PM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] eosinophils on frozen section > > Eric: > My guess is that the alcohol is lysing(blowing up) the unfixed > eosinophils, therefore they are not there to stain. Fixation > stabilizes > the cells in order to be stained in regular paraffin sections. > Unfortunately, I have done a few trials using different fixatives for > fresh tissue to see if I could preserve these little suckers. Nothing > really worked satisfactorally. You might try fixing in formalin for a > minute or two before you continue on with your stain and see if this > helps. (don't forget to wash the sections afterward) Hope this helps. > Claire > > > > We are experiencing some frustration with our ability to identify > eosinophils from frozen sections. For some reason which our frozen > section set up does not enable us to identify eosinophils. Eosinophil > identification is not impaired when the tissue is submitted for > permanents. Could some one please share the reagents used and how the > frozen section staining area is set up if they have success > identifying > eosinophils from a frozen section? We have had many frozen sections > done > on fresh tissues suspicious for an eosinophilic granuloma but our > techniques so far have not rendered consistent eosinophil > identification. We presently are using 95% ETOH for fixing the FS > slide, > quick wash in distilled H2O, 1 minute in Richard Allen Hematoxylin 2, > rinse in tap water, 15 seconds in bluing, rinse in water, rinse in 70% > ETOH, 1 minute in Richard Allen Eosin Y, three rinses in 95% ETOH, > three > rinses in Xylene substitute and then coverslip. All other tissues > appear > just fine but the eosinophils just don't show up. Any suggestions are > greatly appreciated. > > > > > > Eric A. Breisch, Ph.D. > > Clinical Anatomist > > Dept. of Pathology > > Rady Children's Hospital and Health Center > > Associate Clinical Professor of Anatomy > > Dept. of Surgery > > UCSD School of Medicine > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice ** The information contained in this message > may be privileged and is confidential information intended for the > use of the addressee listed above. If you are neither the intended > recipient nor the employee or agent responsible for delivering this > message to the intended recipient, you are hereby notified that any > disclosure, copying, distribution or the taking of any action in > reliance on the contents of this information is strictly prohibited. > If you have received this communication in error, please notify us > immediately by replying to the message and deleting it from your > computer. Thank you. Saint Joseph's Health System, Inc. > > > > ------------------------------ > > Message: 21 > Date: Tue, 5 Feb 2008 17:49:57 -0700 > From: "Gayle Callis" > Subject: Re: [Histonet] Do mice have tonsils?? > To: "Colleen Forster" , > > Message-ID: <000e01c8685a$321bd7d0$6601a8c0@Sunney> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=response > > Colleen, > > Rodents do NOT have tonsils, but have tissues analogous (Sp?) to > tonsils, > called nasal associated lymphoid tissues located in a rather > difficult spot > at the back of the nasal turbinates. I suggest you get into the > literature > and type in murine NALT as there are some excellent articles on > morphology > and their location. If you want to do frozen sections, you either > need to > remove these from this area, not easy to do, and takes a lot of > practice OR > you can do undecalcified bone frozen sections with the Cryojane. > The NALT > is located above the soft palate, just below the 3rd palatine ridge > if you > start counting ridges on the soft palate and starting count at the > front > incisors. These tiny lymphoid tissues are nestled on the roof of > the mouth, > just below the soft palate, between the back molars. The molars are > the > complicating factor for doing murine CD markers that only work on > frozen > sections. I will be happy to send you a powerpoint photograph of a > fully > decalcified mouse head, mid sagittal section, stained with H&E to show > exactly where the NALT is located. I also have a cross section of the > murine nasal turbinates showing NALT in that orientation, and much > harder to > deal with in order to find the NALT. One project had PLP perfused > mouse > head, immersion fixed longer and then EDTA decalcified, > cryoprotected and > sectioned on the cryostat to do immunoglobulin staining of turbinate > epithelial cells back and into the NALT. > > We work with NALT a good deal, and I can honestly say that > dissection/removal is not easy. However, it can be done but with very > gentle, light touch using a pointed, tiny scalpel blade. When we do > frozen > sections of removed NALT, we can obtain approx 30 - 40 sections, > serial, at > 5 um and with treated animals to produce inflammed NALT, up to 50 > and more > serial sections. With undecalcified bone, we get far fewer due to the > difficulty of sectioning. > > G0 into PUBMED, and look for David Pascual, Keri Cscentis on their > NALT > publication. I believe they put a cartoon of NALT location in that > publication. > > Good luck, > > Gayle M. Callis > HTL/HT/MT(ASCP) > Bozeman MT 59715 > > > ----- Original Message ----- > From: "Colleen Forster" > To: > Sent: Tuesday, February 05, 2008 4:36 PM > Subject: [Histonet] Do mice have tonsils?? > > >> For any of you have done mouse necropsy, do they have tonsils or just >> lymph nodes? I have had a request for mouse tonsils.... can't seem >> to find >> them in my searches. I would appreciate any help on this one. >> >> Thanks, >> >> Colleen Forster >> U of MN >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 22 > Date: Tue, 5 Feb 2008 17:58:05 -0700 > From: "Gayle Callis" > Subject: Re: [Histonet] eosinophils on frozen section > To: "Breisch, Eric" , > > Message-ID: <001401c8685b$55212f90$6601a8c0@Sunney> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > We improved our eosinophil staining on frozen sections of murine > tissue by > switching to NBF immersion fixation, let it sit 10 min, then do the > H&E but > use eosin/phloxine (Richard Allan stain will work) but stain in > eosin/phloxine for 2 minutes. For some reason, the eosin was > never taken > up as well after a shorter time in eosin alone, and greatly > improved with > the phloxine added. Sections can be cut a bit thinner too, try 4 um > - it > should make the granules more apparent against the background and > other > cells. Also, I suggest adding absolute alcohol after the 95% for > better > dehydration before clearing. > > You may have success using alcoholic formalin in order to speed up the > fixation a bit. However, when we switched to NBF, we had better > staining of > eosinophils in frozen sections. If you have the time, you can let NBF > fixation go longer, we often let sections sit for days then stain, > but with > your diagnostic procedure, you will want a bit more speed involved. > > Good luck > > Gayle M..Callis > HTL/HT/MT(ASCP) > Bozeman MT 59715 > > > > > > > ----- Original Message ----- > From: "Breisch, Eric" > To: > Sent: Tuesday, February 05, 2008 4:32 PM > Subject: [Histonet] eosinophils on frozen section > > > To all interested Histonetters: > > > > > > We are experiencing some frustration with our ability to identify > eosinophils from frozen sections. For some reason which our frozen > section set up does not enable us to identify eosinophils. Eosinophil > identification is not impaired when the tissue is submitted for > permanents. Could some one please share the reagents used and how the > frozen section staining area is set up if they have success > identifying > eosinophils from a frozen section? We have had many frozen sections > done > on fresh tissues suspicious for an eosinophilic granuloma but our > techniques so far have not rendered consistent eosinophil > identification. We presently are using 95% ETOH for fixing the FS > slide, > quick wash in distilled H2O, 1 minute in Richard Allen Hematoxylin 2, > rinse in tap water, 15 seconds in bluing, rinse in water, rinse in 70% > ETOH, 1 minute in Richard Allen Eosin Y, three rinses in 95% ETOH, > three > rinses in Xylene substitute and then coverslip. All other tissues > appear > just fine but the eosinophils just don't show up. Any suggestions are > greatly appreciated. > > > > > > Eric A. Breisch, Ph.D. > > Clinical Anatomist > > Dept. of Pathology > > Rady Children's Hospital and Health Center > > Associate Clinical Professor of Anatomy > > Dept. of Surgery > > UCSD School of Medicine > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 23 > Date: Tue, 5 Feb 2008 20:06:55 -0600 > From: Julia Dahl > Subject: RE: [Histonet] eosinophils on frozen section > To: "Breisch, Eric" , > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > Agree with Gayle that alcoholic formalin allows for speedier > fixation and preservation of eosinophil granule membranes (and > therefore staining, rather than degranulation). > >> Date: Tue, 5 Feb 2008 15:32:31 -0800 >> From: ebreisch@rchsd.org >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] eosinophils on frozen section >> >> To all interested Histonetters: >> >> >> >> >> >> We are experiencing some frustration with our ability to identify >> eosinophils from frozen sections. For some reason which our frozen >> section set up does not enable us to identify eosinophils. Eosinophil >> identification is not impaired when the tissue is submitted for >> permanents. Could some one please share the reagents used and how the >> frozen section staining area is set up if they have success >> identifying >> eosinophils from a frozen section? We have had many frozen sections >> done >> on fresh tissues suspicious for an eosinophilic granuloma but our >> techniques so far have not rendered consistent eosinophil >> identification. We presently are using 95% ETOH for fixing the FS >> slide, >> quick wash in distilled H2O, 1 minute in Richard Allen Hematoxylin 2, >> rinse in tap water, 15 seconds in bluing, rinse in water, rinse in >> 70% >> ETOH, 1 minute in Richard Allen Eosin Y, three rinses in 95% ETOH, >> three >> rinses in Xylene substitute and then coverslip. All other tissues >> appear >> just fine but the eosinophils just don't show up. Any suggestions are >> greatly appreciated. >> >> >> >> >> >> Eric A. Breisch, Ph.D. >> >> Clinical Anatomist >> >> Dept. of Pathology >> >> Rady Children's Hospital and Health Center >> >> Associate Clinical Professor of Anatomy >> >> Dept. of Surgery >> >> UCSD School of Medicine >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _________________________________________________________________ > Connect and share in new ways with Windows Live. > http://www.windowslive.com/share.html?ocid=TXT_TAGHM_Wave2_sharelife_012008 > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 51, Issue 7 > *************************************** Laura R Hunt, PhD Post-doctoral associate University of Texas at Arlington ph: 817-272-1499 fax: 817-272-2855 lhunt@uta.edu From HornHV <@t> archildrens.org Wed Feb 6 09:37:43 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Feb 6 09:38:16 2008 Subject: [Histonet] OT: the weather In-Reply-To: <08A0A863637F1349BBFD83A96B27A50A1200E1@uwhis-xchng3.uwhis.hosp.wisc.edu> References: <08A0A863637F1349BBFD83A96B27A50A1200E1@uwhis-xchng3.uwhis.hosp.wisc.edu> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82AE8@EMAIL.archildrens.org> Thanks for asking Claire. The count now is 13 people dead from the tornadoes that tore through the state late yesterday and last night. A lot of wind damage to the communities in several Arkansas counties. And many are without electric power. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Wednesday, February 06, 2008 9:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OT: the weather Hey guys: I don't know about you, but I'm really beginning to wonder about the weather. Anyway- everyone in the southern states, especially Arkansas, still there? I gripe about the snow this year, but geez. How bad was the damage from the tornadoes down in that area. Just thinking of everyone, and hoping all is well with everyone. Stay warm/dry/unburnt/etc. You bunch from across the pond can send us your extra show shovels and inflatable rafts. Anywhere you could put us up if it gets too bad over here? :) Claire _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Feb 6 09:43:06 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 6 09:43:10 2008 Subject: [Histonet] Basic Fuchsin vs. Pararosaniline In-Reply-To: <00a601c868ce$a9fcd550$5a96d082@MrDarcy> Message-ID: <437819.15757.qm@web61220.mail.yahoo.com> Basic fuchsin = anilin red = basic rubin = magenta. Is the tri-amino triphenyl methane dyes mixtures of pararosanilin (CI 42500), rosanilin (CI42510) and magenta II in varying proportions. Yes, you can use it. Ren? J. Mareike Heimann wrote: Dear all, I would like to try using the Leder stain for mast cells. Before I buy pararosaniline, which is not available in our lab, I wanted to ask if it is possible to use Basic Fuchsin (which we do have) instead? Thanks in advance for your advice, Mareike Heimann University of Iceland _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From jennifer.l.hofecker <@t> Vanderbilt.Edu Wed Feb 6 09:43:16 2008 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Wed Feb 6 09:43:25 2008 Subject: [Histonet] OT: the weather In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82AE8@EMAIL.archildrens.org> Message-ID: <898D946569A27444B65667A49C07405201564418@mailbe06.mc.vanderbilt.edu> Things were Scary here in TN, especially when a natural gas pumping station exploded! Here's a link for anyone interested: http://www.wsmv.com/weather/15225132/detail.html Thanks To everyone who checked on us (including Claire, via histonet). My family was fortunately not directly affected, but my thoughts and prayers are with the many who were. jennifer -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, February 06, 2008 9:38 AM To: Ingles Claire; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] OT: the weather Thanks for asking Claire. The count now is 13 people dead from the tornadoes that tore through the state late yesterday and last night. A lot of wind damage to the communities in several Arkansas counties. And many are without electric power. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Wednesday, February 06, 2008 9:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OT: the weather Hey guys: I don't know about you, but I'm really beginning to wonder about the weather. Anyway- everyone in the southern states, especially Arkansas, still there? I gripe about the snow this year, but geez. How bad was the damage from the tornadoes down in that area. Just thinking of everyone, and hoping all is well with everyone. Stay warm/dry/unburnt/etc. You bunch from across the pond can send us your extra show shovels and inflatable rafts. Anywhere you could put us up if it gets too bad over here? :) Claire _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Wed Feb 6 09:51:29 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Wed Feb 6 09:51:31 2008 Subject: [Histonet] Cracking of paraffin blocks References: <9D443EB9D0270143B5AAF190CB1A58A30679622B@dogwood.ricerca.com> Message-ID: <002001c868d8$2381c170$6601a8c0@Sunney> Carol, Be sure to stir your paraffin before embedding to redistribute the additives. However your cold plate may be TOO cold. We have had cracks when cooling of paraffin is too rapid and too cold. You may want to turn up the cold plate temperature a bit. I have used the Surgipath infiltration/embedding system, excellent paraffins in the past for animal tissue, and it is not the animal tissue that is causing the problem. The blocks should come out of the molds without any leverage if you are using tools to get then out. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Wilson, Carol" To: Sent: Wednesday, February 06, 2008 6:17 AM Subject: [Histonet] Cracking of paraffin blocks Hi All, Recently we have seen an increase in cracks of paraffin blocks when we take them out of the molds. Sometimes it does not affect the tissue, but there are times that the tissue cracks right along with the paraffin. We are using Surgipath infiltration on our processor and Surgipath embedding media in our embedding center. We use reusable metal base molds. Don't know if would matter or not, but we are processing animal tissue. We take temperatures daily of all wax baths.. nothing out of the ordinary. Any suggestions would be greatly appreciated. Thanks, Carol Carol Wilson Team Leader - Histology Ricerca Biosciences, LLC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Wed Feb 6 10:08:47 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Wed Feb 6 10:08:49 2008 Subject: A gentle reminder about using subject line Re: [Histonet] (no subject) References: <5C0BED61F529364E86309CADEA63FEF2F3AFD0@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <003d01c868da$8e12dfe0$6601a8c0@Sunney> Terry, Whoops, a wording problem here. Correction is "so I don't just delete the message and not read ------" It was a bit unclear by what I meant. I hope this clears up my griping email about no topics in the subject line. Gayle Callis ----- Original Message ----- From: "Marshall Terry Dr, Consultant Histopathologist" To: "Gayle Callis" ; "Margaryan, Naira" ; Sent: Wednesday, February 06, 2008 7:47 AM Subject: RE: A gentle reminder about using subject line Re: [Histonet] (no subject) Gayle, I've never known even a slight tendency to obscurity in your posts, but I'm blowed if I know what you are getting at in this case:-) Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: 06 February 2008 00:30 To: Margaryan, Naira; histonet@lists.utsouthwestern.edu Subject: A gentle reminder about using subject line Re: [Histonet] (no subject) For those new to or not used to the Histonet messaging, please use the subject line rather ant just hit the reply key. Replying to the daily digest also kicks back all the messages for that day to those who have already received them. The subject is important so others, including me, don't just hit the delete key and not read your valuable inquiries and comment. Thanks Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Margaryan, Naira" To: Sent: Tuesday, February 05, 2008 3:50 PM Subject: [Histonet] (no subject) Dear histo team, I need to stain frozen & FFPE sections for Tuj-1 (AbCam, ab53234) and FFPE sectiond for Netrin-1 (AbCam, ab39370). Any kind of suggestions or even full IHC or IF protocol is appreciated. Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ###################################################################### This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. ###################################################################### From Terry.Marshall <@t> rothgen.nhs.uk Wed Feb 6 10:12:57 2008 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Wed Feb 6 10:12:45 2008 Subject: A gentle reminder about using subject line Re: [Histonet] (no subject) Message-ID: <5C0BED61F529364E86309CADEA63FEF2F3AFD7@TRFT-EX01.xRothGen.nhs.uk> Got it:-) Terry -----Original Message----- From: Gayle Callis [mailto:gayle.callis@bresnan.net] Sent: 06 February 2008 16:09 To: Marshall Terry Dr, Consultant Histopathologist; histonet@lists.utsouthwestern.edu Subject: Re: A gentle reminder about using subject line Re: [Histonet] (no subject) Terry, Whoops, a wording problem here. Correction is "so I don't just delete the message and not read ------" It was a bit unclear by what I meant. I hope this clears up my griping email about no topics in the subject line. Gayle Callis ----- Original Message ----- From: "Marshall Terry Dr, Consultant Histopathologist" To: "Gayle Callis" ; "Margaryan, Naira" ; Sent: Wednesday, February 06, 2008 7:47 AM Subject: RE: A gentle reminder about using subject line Re: [Histonet] (no subject) Gayle, I've never known even a slight tendency to obscurity in your posts, but I'm blowed if I know what you are getting at in this case:-) Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: 06 February 2008 00:30 To: Margaryan, Naira; histonet@lists.utsouthwestern.edu Subject: A gentle reminder about using subject line Re: [Histonet] (no subject) For those new to or not used to the Histonet messaging, please use the subject line rather ant just hit the reply key. Replying to the daily digest also kicks back all the messages for that day to those who have already received them. The subject is important so others, including me, don't just hit the delete key and not read your valuable inquiries and comment. Thanks Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Margaryan, Naira" To: Sent: Tuesday, February 05, 2008 3:50 PM Subject: [Histonet] (no subject) Dear histo team, I need to stain frozen & FFPE sections for Tuj-1 (AbCam, ab53234) and FFPE sectiond for Netrin-1 (AbCam, ab39370). Any kind of suggestions or even full IHC or IF protocol is appreciated. Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ###################################################################### This email and any files transmitted with it may contain confidential and privileged information which is intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions expressed are those of the author and do not represent the views of The Rotherham NHS Foundation Trust unless otherwise explicitly stated. The information contained in this email may be subject to public disclosure under the Freedom of Information Act 2000 unless the information is legally exempt from disclosure. The confidentiality of this email and your reply cannot be guaranteed. If you are not the intended recipient please accept our apologies. Please do not disclose, copy or distribute information in this email or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform us that this message has gone astray before deleting it. Thank you for your co-operation. ###################################################################### From pruegg <@t> ihctech.net Wed Feb 6 10:26:03 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Feb 6 10:26:23 2008 Subject: [Histonet] Staining coral tissues embedded in resin In-Reply-To: <5DDB1B77-09A7-4C49-842C-711D3344F608@uta.edu> References: <5DDB1B77-09A7-4C49-842C-711D3344F608@uta.edu> Message-ID: <003601c868dc$fa6cd770$6701a8c0@Patsyoffice> Laura, For H&E's on GMA embedded tissue, you stain right thru the plastic, it is water permeable and cannot be removed, there fore it will often take a more concentrated and or longer incubation times to stain. I use Gill's III hematoxylin for GMA sections for 10 min., you can use alcoholic eosin but beware that alcohol with any water in it tends to lift the GMA section off the slide. Here is what I do: Gill's #3 10 min. Tap wash for 1 min. Dip in ammonia water to blue (10 dips) Tap wash for 5 min. I then airdry the sections before going to alcoholic eosin Alcoholic eosin (right now I am using SurgiPath) 60 dips then quickly 5 dips in 95% alcohol, then to 100% and xylene (once past the alcohol with water the sections are fine. An alternative is to use aqueous eosin, rinse with water, airdry and then dip one at a time in xylene and coverslip. For all other stains that end in water rinses I airdry GMA sections and then coverslip without going thru graded alcohols to dehydrate. Hopes this helps, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laura Hunt Sent: Wednesday, February 06, 2008 8:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Staining coral tissues embedded in resin Hi everyone, I am new to the forum. I am working on coral tissues embedded in Immunobed resin and paraffin. We would like to stain both with H&E and Azure II /Basic Fuchsin. I have never tried staining plastic with H&E. Any suggestions or comments would be great! Since corals have a calcium carbonate skeleton, the tissues were decalcified in a EDTA solution. Laura Hunt UTA On Feb 5, 2008, at 8:07 PM, histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. EpoR antibody (Colleen Forster) > 2. RE: sections falling off (Debrosse-Serra, Beatrice) > 3. Re: sections falling off (Rene J Buesa) > 4. Re: Alcian Blue Quality Control OT (Robert Richmond) > 5. HP staining (Martin, Gary) > 6. Slide printers (ink) (Kim Merriam) > 7. RE: Slide printers (ink) (Charles, Roger) > 8. RE: Slide printers (ink) (Weems, Joyce) > 9. Immunoperoxidase Double staining protocol (Reuel Cornelia) > 10. Re: Slide printers (ink) (Patti Loykasek) > 11. RE: Storage codes (Christine Tambasco) > 12. Re: Slide printers (ink) (pkromund@gundluth.org) > 13. Re: Immunoperoxidase Double staining protocol (Rene J Buesa) > 14. thanks for the support and good wishes (Sebree Linda A.) > 15. (no subject) (Margaryan, Naira) > 16. Do mice have tonsils?? (Colleen Forster) > 17. eosinophils on frozen section (Breisch, Eric) > 18. RE: eosinophils on frozen section (Ingles Claire) > 19. A gentle reminder about using subject line Re: [Histonet] (no > subject) (Gayle Callis) > 20. RE: eosinophils on frozen section (Weems, Joyce) > 21. Re: Do mice have tonsils?? (Gayle Callis) > 22. Re: eosinophils on frozen section (Gayle Callis) > 23. RE: eosinophils on frozen section (Julia Dahl) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 05 Feb 2008 12:20:37 -0600 > From: Colleen Forster > Subject: [Histonet] EpoR antibody > To: histonet@lists.utsouthwestern.edu > Message-ID: <47A8A8F5.3090606@umn.edu> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Hello histonetters, > > Is anyone doing the EpoR antibody on FFPE for IHC? If so, could you > share the vendor and a starting dilution.....thanks. > > Colleen Forster > U of MN > > > > ------------------------------ > > Message: 2 > Date: Tue, 5 Feb 2008 10:30:10 -0800 > From: "Debrosse-Serra, Beatrice" > Subject: RE: [Histonet] sections falling off > To: "Olek Michalski" , "Histonet" > > Message-ID: > > <8404DFBED5207B4B8E5EEF4332CEEA5305BD4C99@lajamrexm01.amer.pfizer.com> > Content-Type: text/plain; charset="us-ascii" > > Try Newcomers silane coated slides. They are not cheap, but the > sections > stay on very well. > > Beatrice DeBrosse-Serra > Pathology Scientist > Pfizer Global Research & Development > CB4, 2150 > 10646 Science Center Drive > San Diego, CA 92121 > Phone# 858-622-5986 > Fax# 858-678-8290 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Olek > Michalski > Sent: Tuesday, February 05, 2008 10:00 AM > To: Histonet > Subject: [Histonet] sections falling off > > > Dear Histonetters, > > a friend of mine just faced a massive sections falling off. She is > doing > > Nissl staining in mouse brain (brains perfused with formalin, > postfixed > 3 > days, and cryosectioned) on poly-L-lysined slides. She just managed to > pass trough xylene and decreasing grades of alcohol to water and the > sections started to detach. We were trying to reattach them but it > went > > out that sections are not flat any more. It seems like the tissue is > rehydrated unevenly, but keeping it in water for hours didn't work. Is > there any way to spread these sections not damaging them at the same > time? > She is likely to rescue this sections even if some work is needed. > > Best regards > Olek Michalski > -- > Laboratory of Neurobiology > of Development and Evolution > > Nencki Institute of Experimental Biology > ul. Pasteura 3, 02-093 Warszawa, Poland > Tel. +48 22 5892268, Fax +48 22 8225342 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 3 > Date: Tue, 5 Feb 2008 10:30:54 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] sections falling off > To: Olek Michalski , Histonet > > Message-ID: <87465.99172.qm@web61214.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > If they were cryosectioned she did not have to go through xylene or > graded alcohols to "dewax and hydrate" since there was no > dehydration or wax infiltration to begin with. > > Cryosectioned sections just need to wash away the medium used to > cryosection (OCT perhaps?) and just stain with the aqueous solutions. > > Later they can de dehydrated and cleared if a permanent mount is > desired. > Try to float the sections in a water bath and pick them up and > don't try again to use xylene or alcohols. > Probably she was following a procedure for paraffin embedded tissue > that should have been adapted to frozen sections and it was not. > Ren? J. > > Olek Michalski wrote: > > Dear Histonetters, > > a friend of mine just faced a massive sections falling off. She is > doing > Nissl staining in mouse brain (brains perfused with formalin, > postfixed 3 > days, and cryosectioned) on poly-L-lysined slides. She just managed to > pass trough xylene and decreasing grades of alcohol to water and the > sections started to detach. We were trying to reattach them but it > went > out that sections are not flat any more. It seems like the tissue is > rehydrated unevenly, but keeping it in water for hours didn't work. Is > there any way to spread these sections not damaging them at the same > time? > She is likely to rescue this sections even if some work is needed. > > Best regards > Olek Michalski > -- > Laboratory of Neurobiology > of Development and Evolution > > Nencki Institute of Experimental Biology > ul. Pasteura 3, 02-093 Warszawa, Poland > Tel. +48 22 5892268, Fax +48 22 8225342 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Looking for last minute shopping deals? Find them fast with Yahoo! > Search. > > ------------------------------ > > Message: 4 > Date: Tue, 5 Feb 2008 13:45:34 -0500 > From: "Robert Richmond" > Subject: [Histonet] Re: Alcian Blue Quality Control OT > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Claire Ingles remarks: So that's what GOP means! > > Not this grumpy old pathologist - I voted for Barack Obama! > > Bob Richmond > Samurai Pathologist > Knoxville TN > > > > ------------------------------ > > Message: 5 > Date: Tue, 5 Feb 2008 11:00:57 -0800 > From: "Martin, Gary" > Subject: [Histonet] HP staining > To: > Message-ID: > <6ED9D4252F278841A0593D3D788AF24C01ACDF72@mailsvr.MARSHMED.local> > Content-Type: text/plain; charset="us-ascii" > > To all who responded to my e-mail concerning rapid HP staining to > include a yellow back ground ... thank you very much ... my question > was > answered. > > Gray > > > > ------------------------------ > > Message: 6 > Date: Tue, 5 Feb 2008 11:48:08 -0800 (PST) > From: Kim Merriam > Subject: [Histonet] Slide printers (ink) > To: Histonet > Message-ID: <813260.30617.qm@web50306.mail.re2.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi everyone, > > I was wondering what everyone's experience was with the TBS slide > printer (Shurmark-plus), the one that prints with ink? I would > appreciate input, good or bad. > > We currently have an etcher, and we hate it, so we are looking to > trade it in for something that uses ink. I have used the Leica > printer in the past and have had reasonably good luck with it, but I > was wondering about the printer from TBS, I don't know anyone that > has one. > > Kim > > > Kim Merriam, MA, HT(ASCP) > Cambridge, MA > > --------------------------------- > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. > Try it now. > > ------------------------------ > > Message: 7 > Date: Tue, 5 Feb 2008 14:58:42 -0500 > From: "Charles, Roger" > Subject: RE: [Histonet] Slide printers (ink) > To: "Kim Merriam" > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > <12E4E17FEF6EBE4BAE95BEB3CDCB57000943BB6E@enhbgpri04.backup> > Content-Type: text/plain; charset="US-ASCII" > > Hello, > Recently we bought a sakura slide writer that uses ink to print. The > only problem I have found with the ink is if you put labels over the > print and then try to remove the label to verify a number error, or > something like that, the ink will actually come off with the label. > If > you can read upside down you can still verify the number. I did run > this ink thru a battery of chemicals including straight formic acid > for > 5 minutes and the ink did not leave the slide. I would caution in > buying a Sakura slide write as it has a very unfriendly Access based > program and the writer does not work with charged slides. This has > been > verified by me and an independent engineer sent to look at this issue. > I do use a TBS cassette writer and love that so if I can get my sakura > sent back I will be looking at TBS's slide writer too. > Good luck > Roger > > Roger Charles > Microbiologist > Pennsylvania Veterinary Laboratory > 2305 N Cameron St > Harrisburg, PA 17110 > 717-787-8808 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim > Merriam > Sent: Tuesday, February 05, 2008 2:48 PM > To: Histonet > Subject: [Histonet] Slide printers (ink) > > Hi everyone, > > I was wondering what everyone's experience was with the TBS slide > printer (Shurmark-plus), the one that prints with ink? I would > appreciate input, good or bad. > > We currently have an etcher, and we hate it, so we are looking to > trade it in for something that uses ink. I have used the Leica > printer > in the past and have had reasonably good luck with it, but I was > wondering about the printer from TBS, I don't know anyone that has > one. > > Kim > > > Kim Merriam, MA, HT(ASCP) > Cambridge, MA > > --------------------------------- > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. > Try > it now. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 8 > Date: Tue, 5 Feb 2008 15:25:58 -0500 > From: "Weems, Joyce" > Subject: RE: [Histonet] Slide printers (ink) > To: "Charles, Roger" , "Kim Merriam" > > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > <1CD6831EB9B26D45B0A3EAA79F7EBD320518E54D@sjhaexc02.sjha.org> > Content-Type: text/plain; charset="us-ascii" > > We have Sakura cassette and slide writers. We print charged slides > daily. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Charles, > Roger > Sent: Tuesday, February 05, 2008 2:59 PM > To: Kim Merriam > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Slide printers (ink) > > Hello, > Recently we bought a sakura slide writer that uses ink to print. The > only problem I have found with the ink is if you put labels over the > print and then try to remove the label to verify a number error, or > something like that, the ink will actually come off with the label. > If > you can read upside down you can still verify the number. I did run > this ink thru a battery of chemicals including straight formic acid > for > 5 minutes and the ink did not leave the slide. I would caution in > buying a Sakura slide write as it has a very unfriendly Access based > program and the writer does not work with charged slides. This has > been > verified by me and an independent engineer sent to look at this issue. > I do use a TBS cassette writer and love that so if I can get my sakura > sent back I will be looking at TBS's slide writer too. > Good luck > Roger > > Roger Charles > Microbiologist > Pennsylvania Veterinary Laboratory > 2305 N Cameron St > Harrisburg, PA 17110 > 717-787-8808 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim > Merriam > Sent: Tuesday, February 05, 2008 2:48 PM > To: Histonet > Subject: [Histonet] Slide printers (ink) > > Hi everyone, > > I was wondering what everyone's experience was with the TBS slide > printer (Shurmark-plus), the one that prints with ink? I would > appreciate input, good or bad. > > We currently have an etcher, and we hate it, so we are looking to > trade it in for something that uses ink. I have used the Leica > printer > in the past and have had reasonably good luck with it, but I was > wondering about the printer from TBS, I don't know anyone that has > one. > > Kim > > > Kim Merriam, MA, HT(ASCP) > Cambridge, MA > > --------------------------------- > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. > Try > it now. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice ** The information contained in this message > may be privileged and is confidential information intended for the > use of the addressee listed above. If you are neither the intended > recipient nor the employee or agent responsible for delivering this > message to the intended recipient, you are hereby notified that any > disclosure, copying, distribution or the taking of any action in > reliance on the contents of this information is strictly prohibited. > If you have received this communication in error, please notify us > immediately by replying to the message and deleting it from your > computer. Thank you. Saint Joseph's Health System, Inc. > > > > ------------------------------ > > Message: 9 > Date: Tue, 05 Feb 2008 14:34:27 -0600 > From: "Reuel Cornelia" > Subject: [Histonet] Immunoperoxidase Double staining protocol > To: > Message-ID: <47A873F3020000C50002ADF9@nwcl02.tsrh.org> > Content-Type: text/plain; charset=US-ASCII > > I was asked to do an immunoperoxidase double staining on CD61 ,Cd42, > pf4 with an unknown mouse antibody that will work on platelets. Can > somebody help me or any protocol available that I can refer to. I am > used of IF double stianing but not Immunoperoxidase. Thank you. > > Reuel Cornelia, BS MT, AMT > Cellular Pathology > Texas Scottish Rite Hospital for Children > 2222 Welborn Street > Dallas, TX 75219 > Tel: 214-559-7766 > fax: 214-559-7768 > > **************************************************************************** *************************************** > Texas Scottish Rite Hospital for Children is one of the nation's > leading pediatric centers for the > treatment of orthopedic conditions, certain related neurological > disorders and learning disorders, > such as dyslexia. This email transmission and/or its attachments > may contain confidential health > information, intended only for the use of the individual or entity > named above. > > The authorized recipient of this information is prohibited from > disclosing it to any other party unless > required to do so by law and is required to delete/destroy the > information after its stated need has > been fulfilled. If you are not the intended recipient, any > disclosure, copying, distribution or action > taken in reliance on the contents of this email transmission is > prohibited. If you have received this > information in error, please notify the sender immediately and > delete this information. > > We appreciate your efforts to protect the children's confidential > information. > **************************************************************************** *************************************** > > > > > > ------------------------------ > > Message: 10 > Date: Tue, 05 Feb 2008 12:39:50 -0800 > From: Patti Loykasek > Subject: Re: [Histonet] Slide printers (ink) > To: "Charles, Roger" , Kim Merriam > > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset="US-ASCII" > > Roger- We have been using a sakura slide printer for a couple of > years. I do > agree with your comments about the software - not user friendly at > all nor > is it intuitive. I have used Access database in the past without these > issues. I do not know about the labels, I don't think we've had that > issue. > Maybe it varies with the label. I don't quite understand your > comment on > charged slides, as all of the slides we put thru the printer are > charged and > we have no issues with that. We use charged slides (Okando Plus) > from BBC > and Probe-On Plus slides from ThermoFisher. What brand of slides > were you > using & what issue did you have with the slide printer from the usage? > > > Patti Loykasek BS, HTL, QIHC > PhenoPath Laboratories > Seattle, WA > > > > > > > >> Hello, >> Recently we bought a sakura slide writer that uses ink to print. The >> only problem I have found with the ink is if you put labels over the >> print and then try to remove the label to verify a number error, or >> something like that, the ink will actually come off with the >> label. If >> you can read upside down you can still verify the number. I did run >> this ink thru a battery of chemicals including straight formic acid >> for >> 5 minutes and the ink did not leave the slide. I would caution in >> buying a Sakura slide write as it has a very unfriendly Access based >> program and the writer does not work with charged slides. This has >> been >> verified by me and an independent engineer sent to look at this >> issue. >> I do use a TBS cassette writer and love that so if I can get my >> sakura >> sent back I will be looking at TBS's slide writer too. >> Good luck >> Roger >> >> Roger Charles >> Microbiologist >> Pennsylvania Veterinary Laboratory >> 2305 N Cameron St >> Harrisburg, PA 17110 >> 717-787-8808 >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim >> Merriam >> Sent: Tuesday, February 05, 2008 2:48 PM >> To: Histonet >> Subject: [Histonet] Slide printers (ink) >> >> Hi everyone, >> >> I was wondering what everyone's experience was with the TBS slide >> printer (Shurmark-plus), the one that prints with ink? I would >> appreciate input, good or bad. >> >> We currently have an etcher, and we hate it, so we are looking to >> trade it in for something that uses ink. I have used the Leica >> printer >> in the past and have had reasonably good luck with it, but I was >> wondering about the printer from TBS, I don't know anyone that has >> one. >> >> Kim >> >> >> Kim Merriam, MA, HT(ASCP) >> Cambridge, MA >> >> --------------------------------- >> Be a better friend, newshound, and know-it-all with Yahoo! Mobile. >> Try >> it now. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > This e-mail message, including any attachments, is for the sole use > of the > intended recipients and may contain privileged information. Any > unauthorized > review, use, disclosure or distribution is prohibited. If you are > not the intended > recipient, please contact the sender by e-mail and destroy all > copies of the > original message, or you may call PhenoPath Laboratories, Seattle, > WA U.S.A. > at (206) 374-9000. > > > > > ------------------------------ > > Message: 11 > Date: Tue, 5 Feb 2008 21:10:37 +0000 > From: Christine Tambasco > Subject: RE: [Histonet] Storage codes > To: Barbara Albert , histonet > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > I always kept my gold chloride in the refrigerator. Yours is in a > vial though so is it a powder? > That is what I used to have I kept at room temperature. > Just my advice. > Christine Tambasco, HT (ASCP) > St. Marys Hospital > Amsterdam, NY> Date: Mon, 4 Feb 2008 15:33:30 -0800> From: barbaraaalbert@yahoo.com > > To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] Storage > codes> > Hi all,> I just received some vials of Gold Chloride that I > had ordered and was checking the label for stoarge requirements. It > has "Storage Code White". I haven't heard of storage codes and want > to know where I can go to find out what they mean.> > Thanks,> > Barbara Albert> UCSF Medical Center> San Francisco> > > > ---------------------------------> Never miss a thing. Make Yahoo > your homepage.> _______________________________________________> > Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _________________________________________________________________ > Climb to the top of the charts! Play the word scramble challenge > with star power. > http://club.live.com/star_shuffle.aspx?icid=starshuffle_wlmailtextlink_jan > > ------------------------------ > > Message: 12 > Date: Tue, 5 Feb 2008 15:34:42 -0600 > From: pkromund@gundluth.org > Subject: Re: [Histonet] Slide printers (ink) > To: Patti Loykasek > Cc: histonet@lists.utsouthwestern.edu, > histonet-bounces@lists.utsouthwestern.edu, "Charles, Roger" > > Message-ID: > > > > Content-Type: text/plain; charset=US-ASCII > > Do any of these slide printers affect precut control tissue on the > charged > slides? > Pamela > > > > Patti Loykasek > > ath.com> To > Sent by: "Charles, Roger" > histonet-bounces@ , Kim > Merriam > lists.utsouthwest > > ern.edu cc > > histonet@lists.utsouthwestern.edu > > Subject > 02/05/2008 02:39 Re: [Histonet] Slide printers > (ink) > PM > > > > > > > > > > Roger- We have been using a sakura slide printer for a couple of > years. I > do > agree with your comments about the software - not user friendly at > all nor > is it intuitive. I have used Access database in the past without these > issues. I do not know about the labels, I don't think we've had that > issue. > Maybe it varies with the label. I don't quite understand your > comment on > charged slides, as all of the slides we put thru the printer are > charged > and > we have no issues with that. We use charged slides (Okando Plus) > from BBC > and Probe-On Plus slides from ThermoFisher. What brand of slides > were you > using & what issue did you have with the slide printer from the usage? > > > Patti Loykasek BS, HTL, QIHC > PhenoPath Laboratories > Seattle, WA > > > > > > > >> Hello, >> Recently we bought a sakura slide writer that uses ink to print. The >> only problem I have found with the ink is if you put labels over the >> print and then try to remove the label to verify a number error, or >> something like that, the ink will actually come off with the >> label. If >> you can read upside down you can still verify the number. I did run >> this ink thru a battery of chemicals including straight formic acid >> for >> 5 minutes and the ink did not leave the slide. I would caution in >> buying a Sakura slide write as it has a very unfriendly Access based >> program and the writer does not work with charged slides. This has >> been >> verified by me and an independent engineer sent to look at this >> issue. >> I do use a TBS cassette writer and love that so if I can get my >> sakura >> sent back I will be looking at TBS's slide writer too. >> Good luck >> Roger >> >> Roger Charles >> Microbiologist >> Pennsylvania Veterinary Laboratory >> 2305 N Cameron St >> Harrisburg, PA 17110 >> 717-787-8808 >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim >> Merriam >> Sent: Tuesday, February 05, 2008 2:48 PM >> To: Histonet >> Subject: [Histonet] Slide printers (ink) >> >> Hi everyone, >> >> I was wondering what everyone's experience was with the TBS slide >> printer (Shurmark-plus), the one that prints with ink? I would >> appreciate input, good or bad. >> >> We currently have an etcher, and we hate it, so we are looking to >> trade it in for something that uses ink. I have used the Leica >> printer >> in the past and have had reasonably good luck with it, but I was >> wondering about the printer from TBS, I don't know anyone that has >> one. >> >> Kim >> >> >> Kim Merriam, MA, HT(ASCP) >> Cambridge, MA >> >> --------------------------------- >> Be a better friend, newshound, and know-it-all with Yahoo! Mobile. >> Try >> it now. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > This e-mail message, including any attachments, is for the sole use > of the > intended recipients and may contain privileged information. Any > unauthorized > review, use, disclosure or distribution is prohibited. If you are > not the > intended > recipient, please contact the sender by e-mail and destroy all > copies of > the > original message, or you may call PhenoPath Laboratories, Seattle, WA > U.S.A. > at (206) 374-9000. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 13 > Date: Tue, 5 Feb 2008 13:46:41 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] Immunoperoxidase Double staining protocol > To: Reuel Cornelia , > histonet@lists.utsouthwestern.edu > Message-ID: <784337.61146.qm@web61225.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Generally speaking you will have to run the whole protocol for one > of the Abs and use one chromogen (of your selection). After that, > since you already did HIER and blocked the internal peroxidase, you > will treat the section with the second Ab, will link/detect it and > use another chromogen that ideally should have a different color. > The first chromogen could be regular DAB and for the second you can > use also DAB but with incorporated nickel that will give a deep/blue > purple color, in contrast with the brown/reddish normal color of the > DAB. > The thing is that you don't need any special protocol, just your > regular IHC protocol for FFPE tissue, run twice with two different > chromogens. > Ren? J. > > Reuel Cornelia wrote: > I was asked to do an immunoperoxidase double staining on > CD61 ,Cd42, pf4 with an unknown mouse antibody that will work on > platelets. Can somebody help me or any protocol available that I can > refer to. I am used of IF double stianing but not Immunoperoxidase. > Thank you. > > Reuel Cornelia, BS MT, AMT > Cellular Pathology > Texas Scottish Rite Hospital for Children > 2222 Welborn Street > Dallas, TX 75219 > Tel: 214-559-7766 > fax: 214-559-7768 > > **************************************************************************** *************************************** > Texas Scottish Rite Hospital for Children is one of the nation's > leading pediatric centers for the > treatment of orthopedic conditions, certain related neurological > disorders and learning disorders, > such as dyslexia. This email transmission and/or its attachments may > contain confidential health > information, intended only for the use of the individual or entity > named above. > > The authorized recipient of this information is prohibited from > disclosing it to any other party unless > required to do so by law and is required to delete/destroy the > information after its stated need has > been fulfilled. If you are not the intended recipient, any > disclosure, copying, distribution or action > taken in reliance on the contents of this email transmission is > prohibited. If you have received this > information in error, please notify the sender immediately and > delete this information. > > We appreciate your efforts to protect the children's confidential > information. > **************************************************************************** *************************************** > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. > Try it now. > > ------------------------------ > > Message: 14 > Date: Tue, 5 Feb 2008 15:47:03 -0600 > From: "Sebree Linda A." > Subject: [Histonet] thanks for the support and good wishes > To: "Histonet" > Message-ID: > > > > Content-Type: text/plain; charset="US-ASCII" > > Hi all, > > Well apparently our CAP inspector took the word of our supervisor on > everything pertaining to our lab, reviewed some of our slides and was > out the door before the weather associated with the "winter storm > warning" arrived! Some times its good to live in the land of ice and > snow! > > Thanks for all the good luck wishes! > > Linda Sebree, HT(ASCP) > University of Wisconsin Hospital & Clinics > IHC/ISH Laboratory > A4/204-3224 > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > FAX: (608)262-7174 > > > > ------------------------------ > > Message: 15 > Date: Tue, 5 Feb 2008 16:50:57 -0600 > From: "Margaryan, Naira" > Subject: [Histonet] (no subject) > To: > Message-ID: > > > > Content-Type: text/plain; charset="us-ascii" > > Dear histo team, > > > > I need to stain frozen & FFPE sections for Tuj-1 (AbCam, ab53234) and > FFPE sectiond for Netrin-1 (AbCam, ab39370). Any kind of suggestions > or > even full IHC or IF protocol is appreciated. > > > > Thanks in advance, > > Naira > > > > > > > > > > ------------------------------ > > Message: 16 > Date: Tue, 05 Feb 2008 17:36:48 -0600 > From: Colleen Forster > Subject: [Histonet] Do mice have tonsils?? > To: histonet@lists.utsouthwestern.edu > Message-ID: <47A8F310.20905@umn.edu> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > For any of you have done mouse necropsy, do they have tonsils or just > lymph nodes? I have had a request for mouse tonsils.... can't seem to > find them in my searches. I would appreciate any help on this one. > > Thanks, > > Colleen Forster > U of MN > > > > ------------------------------ > > Message: 17 > Date: Tue, 5 Feb 2008 15:32:31 -0800 > From: "Breisch, Eric" > Subject: [Histonet] eosinophils on frozen section > To: > Message-ID: > <43B97B4C402C2C44AAA2A8D2C86A88B31A5A78@e2k3backend1.RCHSD.org> > Content-Type: text/plain; charset="US-ASCII" > > To all interested Histonetters: > > > > > > We are experiencing some frustration with our ability to identify > eosinophils from frozen sections. For some reason which our frozen > section set up does not enable us to identify eosinophils. Eosinophil > identification is not impaired when the tissue is submitted for > permanents. Could some one please share the reagents used and how the > frozen section staining area is set up if they have success > identifying > eosinophils from a frozen section? We have had many frozen sections > done > on fresh tissues suspicious for an eosinophilic granuloma but our > techniques so far have not rendered consistent eosinophil > identification. We presently are using 95% ETOH for fixing the FS > slide, > quick wash in distilled H2O, 1 minute in Richard Allen Hematoxylin 2, > rinse in tap water, 15 seconds in bluing, rinse in water, rinse in 70% > ETOH, 1 minute in Richard Allen Eosin Y, three rinses in 95% ETOH, > three > rinses in Xylene substitute and then coverslip. All other tissues > appear > just fine but the eosinophils just don't show up. Any suggestions are > greatly appreciated. > > > > > > Eric A. Breisch, Ph.D. > > Clinical Anatomist > > Dept. of Pathology > > Rady Children's Hospital and Health Center > > Associate Clinical Professor of Anatomy > > Dept. of Surgery > > UCSD School of Medicine > > > > > > ------------------------------ > > Message: 18 > Date: Tue, 5 Feb 2008 18:08:15 -0600 > From: "Ingles Claire" > Subject: RE: [Histonet] eosinophils on frozen section > To: > Message-ID: > <08A0A863637F1349BBFD83A96B27A50A1200DF@uwhis-xchng3.uwhis.hosp.wisc.edu > > > > Content-Type: text/plain; charset="iso-8859-1" > > Eric: > My guess is that the alcohol is lysing(blowing up) the unfixed > eosinophils, therefore they are not there to stain. Fixation > stabilizes the cells in order to be stained in regular paraffin > sections. Unfortunately, I have done a few trials using different > fixatives for fresh tissue to see if I could preserve these little > suckers. Nothing really worked satisfactorally. You might try fixing > in formalin for a minute or two before you continue on with your > stain and see if this helps. (don't forget to wash the sections > afterward) Hope this helps. > Claire > > > > We are experiencing some frustration with our ability to identify > eosinophils from frozen sections. For some reason which our frozen > section set up does not enable us to identify eosinophils. Eosinophil > identification is not impaired when the tissue is submitted for > permanents. Could some one please share the reagents used and how the > frozen section staining area is set up if they have success > identifying > eosinophils from a frozen section? We have had many frozen sections > done > on fresh tissues suspicious for an eosinophilic granuloma but our > techniques so far have not rendered consistent eosinophil > identification. We presently are using 95% ETOH for fixing the FS > slide, > quick wash in distilled H2O, 1 minute in Richard Allen Hematoxylin 2, > rinse in tap water, 15 seconds in bluing, rinse in water, rinse in 70% > ETOH, 1 minute in Richard Allen Eosin Y, three rinses in 95% ETOH, > three > rinses in Xylene substitute and then coverslip. All other tissues > appear > just fine but the eosinophils just don't show up. Any suggestions are > greatly appreciated. > > > > > > Eric A. Breisch, Ph.D. > > Clinical Anatomist > > Dept. of Pathology > > Rady Children's Hospital and Health Center > > Associate Clinical Professor of Anatomy > > Dept. of Surgery > > UCSD School of Medicine > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 19 > Date: Tue, 5 Feb 2008 17:30:17 -0700 > From: "Gayle Callis" > Subject: A gentle reminder about using subject line Re: [Histonet] (no > subject) > To: "Margaryan, Naira" , > > Message-ID: <000601c86857$72c69700$6601a8c0@Sunney> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > For those new to or not used to the Histonet messaging, please use the > subject line rather ant just hit the reply key. Replying to the daily > digest also kicks back all the messages for that day to those who have > already received them. The subject is important so others, including > me, > don't just hit the delete key and not read your valuable inquiries and > comment. > > Thanks > Gayle M. Callis > HTL/HT/MT(ASCP) > Bozeman MT 59715 > > > ----- Original Message ----- > From: "Margaryan, Naira" > To: > Sent: Tuesday, February 05, 2008 3:50 PM > Subject: [Histonet] (no subject) > > > Dear histo team, > > > > I need to stain frozen & FFPE sections for Tuj-1 (AbCam, ab53234) and > FFPE sectiond for Netrin-1 (AbCam, ab39370). Any kind of suggestions > or > even full IHC or IF protocol is appreciated. > > > > Thanks in advance, > > Naira > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 20 > Date: Tue, 5 Feb 2008 19:35:12 -0500 > From: "Weems, Joyce" > Subject: RE: [Histonet] eosinophils on frozen section > To: "Ingles Claire" , > > Message-ID: > <1CD6831EB9B26D45B0A3EAA79F7EBD320518E56A@sjhaexc02.sjha.org> > Content-Type: text/plain; charset="us-ascii" > > Fix in formal-alcohol (no, it is not wearing a tuxedo) 90 ml absolute > alcohol - 10 ml 37% formaldehyde... Should work ok then... j > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles > Claire > Sent: Tuesday, February 05, 2008 7:08 PM > To: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] eosinophils on frozen section > > Eric: > My guess is that the alcohol is lysing(blowing up) the unfixed > eosinophils, therefore they are not there to stain. Fixation > stabilizes > the cells in order to be stained in regular paraffin sections. > Unfortunately, I have done a few trials using different fixatives for > fresh tissue to see if I could preserve these little suckers. Nothing > really worked satisfactorally. You might try fixing in formalin for a > minute or two before you continue on with your stain and see if this > helps. (don't forget to wash the sections afterward) Hope this helps. > Claire > > > > We are experiencing some frustration with our ability to identify > eosinophils from frozen sections. For some reason which our frozen > section set up does not enable us to identify eosinophils. Eosinophil > identification is not impaired when the tissue is submitted for > permanents. Could some one please share the reagents used and how the > frozen section staining area is set up if they have success > identifying > eosinophils from a frozen section? We have had many frozen sections > done > on fresh tissues suspicious for an eosinophilic granuloma but our > techniques so far have not rendered consistent eosinophil > identification. We presently are using 95% ETOH for fixing the FS > slide, > quick wash in distilled H2O, 1 minute in Richard Allen Hematoxylin 2, > rinse in tap water, 15 seconds in bluing, rinse in water, rinse in 70% > ETOH, 1 minute in Richard Allen Eosin Y, three rinses in 95% ETOH, > three > rinses in Xylene substitute and then coverslip. All other tissues > appear > just fine but the eosinophils just don't show up. Any suggestions are > greatly appreciated. > > > > > > Eric A. Breisch, Ph.D. > > Clinical Anatomist > > Dept. of Pathology > > Rady Children's Hospital and Health Center > > Associate Clinical Professor of Anatomy > > Dept. of Surgery > > UCSD School of Medicine > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice ** The information contained in this message > may be privileged and is confidential information intended for the > use of the addressee listed above. If you are neither the intended > recipient nor the employee or agent responsible for delivering this > message to the intended recipient, you are hereby notified that any > disclosure, copying, distribution or the taking of any action in > reliance on the contents of this information is strictly prohibited. > If you have received this communication in error, please notify us > immediately by replying to the message and deleting it from your > computer. Thank you. Saint Joseph's Health System, Inc. > > > > ------------------------------ > > Message: 21 > Date: Tue, 5 Feb 2008 17:49:57 -0700 > From: "Gayle Callis" > Subject: Re: [Histonet] Do mice have tonsils?? > To: "Colleen Forster" , > > Message-ID: <000e01c8685a$321bd7d0$6601a8c0@Sunney> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=response > > Colleen, > > Rodents do NOT have tonsils, but have tissues analogous (Sp?) to > tonsils, > called nasal associated lymphoid tissues located in a rather > difficult spot > at the back of the nasal turbinates. I suggest you get into the > literature > and type in murine NALT as there are some excellent articles on > morphology > and their location. If you want to do frozen sections, you either > need to > remove these from this area, not easy to do, and takes a lot of > practice OR > you can do undecalcified bone frozen sections with the Cryojane. > The NALT > is located above the soft palate, just below the 3rd palatine ridge > if you > start counting ridges on the soft palate and starting count at the > front > incisors. These tiny lymphoid tissues are nestled on the roof of > the mouth, > just below the soft palate, between the back molars. The molars are > the > complicating factor for doing murine CD markers that only work on > frozen > sections. I will be happy to send you a powerpoint photograph of a > fully > decalcified mouse head, mid sagittal section, stained with H&E to show > exactly where the NALT is located. I also have a cross section of the > murine nasal turbinates showing NALT in that orientation, and much > harder to > deal with in order to find the NALT. One project had PLP perfused > mouse > head, immersion fixed longer and then EDTA decalcified, > cryoprotected and > sectioned on the cryostat to do immunoglobulin staining of turbinate > epithelial cells back and into the NALT. > > We work with NALT a good deal, and I can honestly say that > dissection/removal is not easy. However, it can be done but with very > gentle, light touch using a pointed, tiny scalpel blade. When we do > frozen > sections of removed NALT, we can obtain approx 30 - 40 sections, > serial, at > 5 um and with treated animals to produce inflammed NALT, up to 50 > and more > serial sections. With undecalcified bone, we get far fewer due to the > difficulty of sectioning. > > G0 into PUBMED, and look for David Pascual, Keri Cscentis on their > NALT > publication. I believe they put a cartoon of NALT location in that > publication. > > Good luck, > > Gayle M. Callis > HTL/HT/MT(ASCP) > Bozeman MT 59715 > > > ----- Original Message ----- > From: "Colleen Forster" > To: > Sent: Tuesday, February 05, 2008 4:36 PM > Subject: [Histonet] Do mice have tonsils?? > > >> For any of you have done mouse necropsy, do they have tonsils or just >> lymph nodes? I have had a request for mouse tonsils.... can't seem >> to find >> them in my searches. I would appreciate any help on this one. >> >> Thanks, >> >> Colleen Forster >> U of MN >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 22 > Date: Tue, 5 Feb 2008 17:58:05 -0700 > From: "Gayle Callis" > Subject: Re: [Histonet] eosinophils on frozen section > To: "Breisch, Eric" , > > Message-ID: <001401c8685b$55212f90$6601a8c0@Sunney> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > We improved our eosinophil staining on frozen sections of murine > tissue by > switching to NBF immersion fixation, let it sit 10 min, then do the > H&E but > use eosin/phloxine (Richard Allan stain will work) but stain in > eosin/phloxine for 2 minutes. For some reason, the eosin was > never taken > up as well after a shorter time in eosin alone, and greatly > improved with > the phloxine added. Sections can be cut a bit thinner too, try 4 um > - it > should make the granules more apparent against the background and > other > cells. Also, I suggest adding absolute alcohol after the 95% for > better > dehydration before clearing. > > You may have success using alcoholic formalin in order to speed up the > fixation a bit. However, when we switched to NBF, we had better > staining of > eosinophils in frozen sections. If you have the time, you can let NBF > fixation go longer, we often let sections sit for days then stain, > but with > your diagnostic procedure, you will want a bit more speed involved. > > Good luck > > Gayle M..Callis > HTL/HT/MT(ASCP) > Bozeman MT 59715 > > > > > > > ----- Original Message ----- > From: "Breisch, Eric" > To: > Sent: Tuesday, February 05, 2008 4:32 PM > Subject: [Histonet] eosinophils on frozen section > > > To all interested Histonetters: > > > > > > We are experiencing some frustration with our ability to identify > eosinophils from frozen sections. For some reason which our frozen > section set up does not enable us to identify eosinophils. Eosinophil > identification is not impaired when the tissue is submitted for > permanents. Could some one please share the reagents used and how the > frozen section staining area is set up if they have success > identifying > eosinophils from a frozen section? We have had many frozen sections > done > on fresh tissues suspicious for an eosinophilic granuloma but our > techniques so far have not rendered consistent eosinophil > identification. We presently are using 95% ETOH for fixing the FS > slide, > quick wash in distilled H2O, 1 minute in Richard Allen Hematoxylin 2, > rinse in tap water, 15 seconds in bluing, rinse in water, rinse in 70% > ETOH, 1 minute in Richard Allen Eosin Y, three rinses in 95% ETOH, > three > rinses in Xylene substitute and then coverslip. All other tissues > appear > just fine but the eosinophils just don't show up. Any suggestions are > greatly appreciated. > > > > > > Eric A. Breisch, Ph.D. > > Clinical Anatomist > > Dept. of Pathology > > Rady Children's Hospital and Health Center > > Associate Clinical Professor of Anatomy > > Dept. of Surgery > > UCSD School of Medicine > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 23 > Date: Tue, 5 Feb 2008 20:06:55 -0600 > From: Julia Dahl > Subject: RE: [Histonet] eosinophils on frozen section > To: "Breisch, Eric" , > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > Agree with Gayle that alcoholic formalin allows for speedier > fixation and preservation of eosinophil granule membranes (and > therefore staining, rather than degranulation). > >> Date: Tue, 5 Feb 2008 15:32:31 -0800 >> From: ebreisch@rchsd.org >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] eosinophils on frozen section >> >> To all interested Histonetters: >> >> >> >> >> >> We are experiencing some frustration with our ability to identify >> eosinophils from frozen sections. For some reason which our frozen >> section set up does not enable us to identify eosinophils. Eosinophil >> identification is not impaired when the tissue is submitted for >> permanents. Could some one please share the reagents used and how the >> frozen section staining area is set up if they have success >> identifying >> eosinophils from a frozen section? We have had many frozen sections >> done >> on fresh tissues suspicious for an eosinophilic granuloma but our >> techniques so far have not rendered consistent eosinophil >> identification. We presently are using 95% ETOH for fixing the FS >> slide, >> quick wash in distilled H2O, 1 minute in Richard Allen Hematoxylin 2, >> rinse in tap water, 15 seconds in bluing, rinse in water, rinse in >> 70% >> ETOH, 1 minute in Richard Allen Eosin Y, three rinses in 95% ETOH, >> three >> rinses in Xylene substitute and then coverslip. All other tissues >> appear >> just fine but the eosinophils just don't show up. Any suggestions are >> greatly appreciated. >> >> >> >> >> >> Eric A. Breisch, Ph.D. >> >> Clinical Anatomist >> >> Dept. of Pathology >> >> Rady Children's Hospital and Health Center >> >> Associate Clinical Professor of Anatomy >> >> Dept. of Surgery >> >> UCSD School of Medicine >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _________________________________________________________________ > Connect and share in new ways with Windows Live. > http://www.windowslive.com/share.html?ocid=TXT_TAGHM_Wave2_sharelife_012008 > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 51, Issue 7 > *************************************** Laura R Hunt, PhD Post-doctoral associate University of Texas at Arlington ph: 817-272-1499 fax: 817-272-2855 lhunt@uta.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo <@t> f2s.com Wed Feb 6 10:26:36 2008 From: kemlo <@t> f2s.com (kemlo) Date: Wed Feb 6 10:27:30 2008 Subject: [Histonet] OT: the weather In-Reply-To: <08A0A863637F1349BBFD83A96B27A50A1200E1@uwhis-xchng3.uwhis.hosp.wisc.edu> References: <08A0A863637F1349BBFD83A96B27A50A1200E1@uwhis-xchng3.uwhis.hosp.wisc.edu> Message-ID: We across the pond are constructing an Ark; there's enough rain to float it and enough wind to give it the kinetic energy it requires. I blame...... The no smoking policy. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: 06 February 2008 15:06 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OT: the weather Hey guys: I don't know about you, but I'm really beginning to wonder about the weather. Anyway- everyone in the southern states, especially Arkansas, still there? I gripe about the snow this year, but geez. How bad was the damage from the tornadoes down in that area. Just thinking of everyone, and hoping all is well with everyone. Stay warm/dry/unburnt/etc. You bunch from across the pond can send us your extra show shovels and inflatable rafts. Anywhere you could put us up if it gets too bad over here? :) Claire _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mlgiebel <@t> vcu.edu Wed Feb 6 10:34:40 2008 From: mlgiebel <@t> vcu.edu (Mary L Giebel/FS/VCU) Date: Wed Feb 6 10:34:47 2008 Subject: [Histonet] Tissue processor info requested Message-ID: I would appreciate your thoughts and advice on tissue processors, both conventional and microwave. I have just been notified that I may be able to purchase a tissue processor...and they would like the quote by tomorrow morning. My previous research on processors is now outdated. I run a small research histology core with a low volume and a low budget. I mainly work with mouse tissue (heart and lungs). Most of the tissue that I work with is used for IHC, which is why I am somewhat hesitant to go with a microwave processor. I have found a unit that looks interesting, the Microm STP-120, but I am concerned because it uses a centrifugal cycle. That would not be a problem with the hearts, but the lungs that I work with are inflated. Has anyone had a problem with the unit? Any and all help would be greatly appreciated. Thank you. Regards, Mary Lee Giebel From Jackie.O'Connor <@t> abbott.com Wed Feb 6 10:37:43 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed Feb 6 10:38:03 2008 Subject: [Histonet] OT: the weather In-Reply-To: Message-ID: Up here in Illinois near the Wisconsin border - we're expecting 12-14 inches of snow, and most of the staff have been allowed to go home. I just read that Hawaii has had the worst rainfall in decades, 12" of rain in 24 hours in Hilo - still not enough to extinguish the volcano, tho. (Yes, I am kidding about the volcano). "kemlo" Sent by: histonet-bounces@lists.utsouthwestern.edu 02/06/2008 10:26 AM To "'Ingles Claire'" cc histonet@lists.utsouthwestern.edu Subject RE: [Histonet] OT: the weather We across the pond are constructing an Ark; there's enough rain to float it and enough wind to give it the kinetic energy it requires. I blame...... The no smoking policy. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: 06 February 2008 15:06 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OT: the weather Hey guys: I don't know about you, but I'm really beginning to wonder about the weather. Anyway- everyone in the southern states, especially Arkansas, still there? I gripe about the snow this year, but geez. How bad was the damage from the tornadoes down in that area. Just thinking of everyone, and hoping all is well with everyone. Stay warm/dry/unburnt/etc. You bunch from across the pond can send us your extra show shovels and inflatable rafts. Anywhere you could put us up if it gets too bad over here? :) Claire _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maxim_71 <@t> mail.ru Wed Feb 6 10:41:43 2008 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Wed Feb 6 10:44:02 2008 Subject: [Histonet] eosinophils on frozen section Message-ID: <373724620.20080206194143@mail.ru> Eric: Try Giemsa in any modification. pH is critical, better approximately 5.5-6. It is longer than H&E (1 h or so), but you will have in result as eosinophils as mast cells. Dehydrate through acetone, clearing and coverslip as ususal. Sincerely, Maxim Peshkov Taganrog, Russia. From mpence <@t> grhs.net Wed Feb 6 10:57:03 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Feb 6 10:57:09 2008 Subject: [Histonet] Tissue processor info requested In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A370A@IS-E2K3.grhs.net> Ask for a demo if that is the processor you like. I am demoing a Peloris from Leica at this time. Processes great. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mary L Giebel/FS/VCU Sent: Wednesday, February 06, 2008 10:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue processor info requested I would appreciate your thoughts and advice on tissue processors, both conventional and microwave. I have just been notified that I may be able to purchase a tissue processor...and they would like the quote by tomorrow morning. My previous research on processors is now outdated. I run a small research histology core with a low volume and a low budget. I mainly work with mouse tissue (heart and lungs). Most of the tissue that I work with is used for IHC, which is why I am somewhat hesitant to go with a microwave processor. I have found a unit that looks interesting, the Microm STP-120, but I am concerned because it uses a centrifugal cycle. That would not be a problem with the hearts, but the lungs that I work with are inflated. Has anyone had a problem with the unit? Any and all help would be greatly appreciated. Thank you. Regards, Mary Lee Giebel _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jvanpatten <@t> cytocoreinc.com Wed Feb 6 11:01:47 2008 From: jvanpatten <@t> cytocoreinc.com (Jennifer Van Patten) Date: Wed Feb 6 10:59:45 2008 Subject: [Histonet] OT: the weather References: Message-ID: Strange weather across the country! Even Maui and the "Big Island" got snow 1 week ago... http://starbulletin.com/2008/01/30/news/story01.html -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Wednesday, February 06, 2008 10:38 AM To: kemlo Cc: histonet@lists.utsouthwestern.edu; 'Ingles Claire' Subject: RE: [Histonet] OT: the weather Up here in Illinois near the Wisconsin border - we're expecting 12-14 inches of snow, and most of the staff have been allowed to go home. I just read that Hawaii has had the worst rainfall in decades, 12" of rain in 24 hours in Hilo - still not enough to extinguish the volcano, tho. (Yes, I am kidding about the volcano). "kemlo" Sent by: histonet-bounces@lists.utsouthwestern.edu 02/06/2008 10:26 AM To "'Ingles Claire'" cc histonet@lists.utsouthwestern.edu Subject RE: [Histonet] OT: the weather We across the pond are constructing an Ark; there's enough rain to float it and enough wind to give it the kinetic energy it requires. I blame...... The no smoking policy. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: 06 February 2008 15:06 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OT: the weather Hey guys: I don't know about you, but I'm really beginning to wonder about the weather. Anyway- everyone in the southern states, especially Arkansas, still there? I gripe about the snow this year, but geez. How bad was the damage from the tornadoes down in that area. Just thinking of everyone, and hoping all is well with everyone. Stay warm/dry/unburnt/etc. You bunch from across the pond can send us your extra show shovels and inflatable rafts. Anywhere you could put us up if it gets too bad over here? :) Claire _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From olek.michalski <@t> nencki.gov.pl Wed Feb 6 11:08:54 2008 From: olek.michalski <@t> nencki.gov.pl (Olek Michalski) Date: Wed Feb 6 11:02:52 2008 Subject: [Histonet] sections falling off In-Reply-To: <87465.99172.qm@web61214.mail.yahoo.com> References: <87465.99172.qm@web61214.mail.yahoo.com> Message-ID: Well, what can I say? It seemed a little silly to me too but I was told this step is for removing fat (myelin) which does not allow a good contrast in Nissl staining. Nevetheless most of us use chlorofom/ethanol mixture instead of xylene as a first step and I have never seen such a strange "ruffling" on sections processed this way even if sections fall off sometimes. It is common here to cut strongly fixed tissue. It is collected in PBS filled wells or on gelatin covered slides. In the latter case the sections are spread using brush and drop of buffer and air-dried (no alcohol stage between cutting and drying). Maybe it would be better to keep them in higher temperature for some time as suggested Mary? I am afraid that the problem is in too strong fixation. Any comments are wellcome. Olek Michalski 2008-02-05 19:30:54 Rene J Buesa wrote: > If they were cryosectioned she did not have to go through xylene or > graded alcohols to "dewax and hydrate" since there was no dehydration or > wax infiltration to begin with. > Cryosectioned sections just need to wash away the medium used to > cryosection (OCT perhaps?) and just stain with the aqueous solutions. > Later they can de dehydrated and cleared if a permanent mount is > desired. > Try to float the sections in a water bath and pick them up and don't > try again to use xylene or alcohols. > Probably she was following a procedure for paraffin embedded tissue > that should have been adapted to frozen sections and it was not. > Ren? J. > > Olek Michalski wrote: > Dear Histonetters, > > a friend of mine just faced a massive sections falling off. She is doing > Nissl staining in mouse brain (brains perfused with formalin, postfixed 3 > days, and cryosectioned) on poly-L-lysined slides. She just managed to > pass trough xylene and decreasing grades of alcohol to water and the > sections started to detach. We were trying to reattach them but it went > out that sections are not flat any more. It seems like the tissue is > rehydrated unevenly, but keeping it in water for hours didn't work. Is > there any way to spread these sections not damaging them at the same > time? > She is likely to rescue this sections even if some work is needed. > > Best regards > Olek Michalski From susanbachus <@t> verizon.net Wed Feb 6 11:11:19 2008 From: susanbachus <@t> verizon.net (Susan Bachus) Date: Wed Feb 6 11:17:02 2008 Subject: [Histonet] sections falling off References: <87465.99172.qm@web61214.mail.yahoo.com> Message-ID: <000301c868e3$4b10b470$2e01a8c0@RESLAPTOP> I know that poly-L-lysined slides are supposed to work for this, but I have NEVER seen sx's fall off good old-fashioned double-gelatin-subbed slides under any conditions! Might be worth a try. Susan ----- Original Message ----- From: "Olek Michalski" To: "Histonet" Sent: Wednesday, February 06, 2008 12:08 PM Subject: Re: [Histonet] sections falling off Well, what can I say? It seemed a little silly to me too but I was told this step is for removing fat (myelin) which does not allow a good contrast in Nissl staining. Nevetheless most of us use chlorofom/ethanol mixture instead of xylene as a first step and I have never seen such a strange "ruffling" on sections processed this way even if sections fall off sometimes. It is common here to cut strongly fixed tissue. It is collected in PBS filled wells or on gelatin covered slides. In the latter case the sections are spread using brush and drop of buffer and air-dried (no alcohol stage between cutting and drying). Maybe it would be better to keep them in higher temperature for some time as suggested Mary? I am afraid that the problem is in too strong fixation. Any comments are wellcome. Olek Michalski 2008-02-05 19:30:54 Rene J Buesa wrote: > If they were cryosectioned she did not have to go through xylene or > graded alcohols to "dewax and hydrate" since there was no dehydration or > wax infiltration to begin with. > Cryosectioned sections just need to wash away the medium used to > cryosection (OCT perhaps?) and just stain with the aqueous solutions. > Later they can de dehydrated and cleared if a permanent mount is > desired. > Try to float the sections in a water bath and pick them up and don't > try again to use xylene or alcohols. > Probably she was following a procedure for paraffin embedded tissue > that should have been adapted to frozen sections and it was not. > Ren? J. > > Olek Michalski wrote: > Dear Histonetters, > > a friend of mine just faced a massive sections falling off. She is doing > Nissl staining in mouse brain (brains perfused with formalin, postfixed 3 > days, and cryosectioned) on poly-L-lysined slides. She just managed to > pass trough xylene and decreasing grades of alcohol to water and the > sections started to detach. We were trying to reattach them but it went > out that sections are not flat any more. It seems like the tissue is > rehydrated unevenly, but keeping it in water for hours didn't work. Is > there any way to spread these sections not damaging them at the same > time? > She is likely to rescue this sections even if some work is needed. > > Best regards > Olek Michalski _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Wed Feb 6 11:30:31 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Feb 6 11:28:27 2008 Subject: [Histonet] Tissue processor info requested In-Reply-To: References: Message-ID: <1F937FB30BDB7C4A9F39F83FEA8D379F3E4F3D@bruexchange1.digestivespecialists.com> Mary Lee, I use to have the Microm 120. I don't know about your inflated lungs but I never had a problem on the delicate GI biopsies. Be sure you have good ventilation though because it does raise the tissue basket and move it to the next station. This leaves all of the reagents open to the air. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mary L Giebel/FS/VCU Sent: Wednesday, February 06, 2008 11:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue processor info requested I would appreciate your thoughts and advice on tissue processors, both conventional and microwave. I have just been notified that I may be able to purchase a tissue processor...and they would like the quote by tomorrow morning. My previous research on processors is now outdated. I run a small research histology core with a low volume and a low budget. I mainly work with mouse tissue (heart and lungs). Most of the tissue that I work with is used for IHC, which is why I am somewhat hesitant to go with a microwave processor. I have found a unit that looks interesting, the Microm STP-120, but I am concerned because it uses a centrifugal cycle. That would not be a problem with the hearts, but the lungs that I work with are inflated. Has anyone had a problem with the unit? Any and all help would be greatly appreciated. Thank you. Regards, Mary Lee Giebel _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Adam.T.Anthony <@t> kp.org Wed Feb 6 12:01:32 2008 From: Adam.T.Anthony <@t> kp.org (Adam.T.Anthony@kp.org) Date: Wed Feb 6 12:02:08 2008 Subject: [Histonet] Adam T Anthony/CA/KAIPERM is out of the office. Message-ID: I will be out of the office starting Wed 02/06/2008 and will not return until Thu 02/07/2008. I will be out of the office Wednesday February 6th and returning Thursday, February 7th. I will respond to your message when I return. Please contact Jonathan Nubla for assistance while I am out (415-833-6480, jonathan.nubla@kp.org). From sbreeden <@t> nmda.nmsu.edu Wed Feb 6 12:35:42 2008 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Feb 6 12:35:50 2008 Subject: [Histonet] Cutting Plant Material Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E6169@nmdamailsvr.nmda.ad.nmsu.edu> I've been asked to help a museum employee produce some slides of excavated plant material (yucca, etc.) but before I plunge in to processing and cutting, is there a source of information that would help? The material is, obviously, very desiccated and as it is fibrous, I'm really pondering how I can produce a section that would be a help. Your help would be ah... helpful! Thanks! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From juditw <@t> u.washington.edu Wed Feb 6 12:41:21 2008 From: juditw <@t> u.washington.edu (Judith L. Williams) Date: Wed Feb 6 12:41:27 2008 Subject: [Histonet] OT: the weather In-Reply-To: <898D946569A27444B65667A49C07405201564418@mailbe06.mc.vanderbilt.edu> Message-ID: hi to all affected by the tornados----- if there is anything you need or you know of what folks need please let us know!!!! I am in Washington state after Hurricane Katrina - so am particularly sensitive to natural disasters! with regards, Judy in Washington Dept. of Comparative Medicine On Wed, 6 Feb 2008, Hofecker, Jennifer L wrote: > Things were Scary here in TN, especially when a natural gas pumping > station exploded! > Here's a link for anyone interested: > http://www.wsmv.com/weather/15225132/detail.html > > Thanks To everyone who checked on us (including Claire, via histonet). > My family was fortunately not directly affected, but my thoughts and > prayers are with the many who were. > jennifer > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, > Hazel V > Sent: Wednesday, February 06, 2008 9:38 AM > To: Ingles Claire; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] OT: the weather > > > Thanks for asking Claire. The count now is 13 people dead from the > tornadoes that tore through the state late yesterday and last night. A > lot of wind damage to the communities in several Arkansas counties. > And many are without electric power. > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Histology > Arkansas Children's Hospital > 800 Marshall Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax 501.364.3912 > > visit us on the web at: www.archildrens.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles > Claire > Sent: Wednesday, February 06, 2008 9:06 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] OT: the weather > > Hey guys: > I don't know about you, but I'm really beginning to wonder about the > weather. Anyway- everyone in the southern states, especially Arkansas, > still there? I gripe about the snow this year, but geez. How bad was the > damage from the tornadoes down in that area. Just thinking of everyone, > and hoping all is well with everyone. Stay warm/dry/unburnt/etc. You > bunch from across the pond can send us your extra show shovels and > inflatable rafts. Anywhere you could put us up if it gets too bad over > here? :) Claire > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lblazek <@t> digestivespecialists.com Wed Feb 6 13:06:30 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Feb 6 13:04:31 2008 Subject: [Histonet] Cutting Plant Material In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E6169@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B017E6169@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <1F937FB30BDB7C4A9F39F83FEA8D379F3E4F41@bruexchange1.digestivespecialists.com> Desiccated yucca .... yum sounds wonderful -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, February 06, 2008 1:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cutting Plant Material I've been asked to help a museum employee produce some slides of excavated plant material (yucca, etc.) but before I plunge in to processing and cutting, is there a source of information that would help? The material is, obviously, very desiccated and as it is fibrous, I'm really pondering how I can produce a section that would be a help. Your help would be ah... helpful! Thanks! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Wed Feb 6 14:06:24 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Feb 6 14:06:39 2008 Subject: [Histonet] OT: the weather In-Reply-To: Message-ID: <000201c868fb$c33a5880$d00f7ca5@lurie.northwestern.edu> Well said Claire. I'm in Chicago so the city will see 9 or so though I could have ice skated here on the layer of sleet and slush on the sidewalks. I live in the far south burbs so not so much (this time). We're still hanging on- glad I take public transport. Kemlo- send the ark! Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Wednesday, February 06, 2008 10:38 AM To: kemlo Cc: histonet@lists.utsouthwestern.edu; 'Ingles Claire' Subject: RE: [Histonet] OT: the weather Up here in Illinois near the Wisconsin border - we're expecting 12-14 inches of snow, and most of the staff have been allowed to go home. I just read that Hawaii has had the worst rainfall in decades, 12" of rain in 24 hours in Hilo - still not enough to extinguish the volcano, tho. (Yes, I am kidding about the volcano). "kemlo" Sent by: histonet-bounces@lists.utsouthwestern.edu 02/06/2008 10:26 AM To "'Ingles Claire'" cc histonet@lists.utsouthwestern.edu Subject RE: [Histonet] OT: the weather We across the pond are constructing an Ark; there's enough rain to float it and enough wind to give it the kinetic energy it requires. I blame...... The no smoking policy. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: 06 February 2008 15:06 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OT: the weather Hey guys: I don't know about you, but I'm really beginning to wonder about the weather. Anyway- everyone in the southern states, especially Arkansas, still there? I gripe about the snow this year, but geez. How bad was the damage from the tornadoes down in that area. Just thinking of everyone, and hoping all is well with everyone. Stay warm/dry/unburnt/etc. You bunch from across the pond can send us your extra show shovels and inflatable rafts. Anywhere you could put us up if it gets too bad over here? :) Claire _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Feb 6 14:21:15 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 6 14:21:19 2008 Subject: [Histonet] Cutting Plant Material In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E6169@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <529676.12079.qm@web61224.mail.yahoo.com> Yuca, as well as other tubers, contain a lot of starch and they are a source of problems. First fix in FAA (Formil Acetic Acid), prepare THIN slices and dehydrate with EthOL. You can try clearing it with xylene, but when I did this procedure (56 years ago) I cleared with aniline oil followed by a short immesion in benzene. What you have to use is a paraffin of as high melting point as possible (63-65?C). Bolles Lee have some procedures, as well as Gary's. Ren? J. "Breeden, Sara" wrote: I've been asked to help a museum employee produce some slides of excavated plant material (yucca, etc.) but before I plunge in to processing and cutting, is there a source of information that would help? The material is, obviously, very desiccated and as it is fibrous, I'm really pondering how I can produce a section that would be a help. Your help would be ah... helpful! Thanks! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From JGREWE <@t> OhioHealth.com Wed Feb 6 15:02:02 2008 From: JGREWE <@t> OhioHealth.com (JGREWE@OhioHealth.com) Date: Wed Feb 6 15:02:15 2008 Subject: [Histonet] Jacquelyn Grewe/Staff/OhioHealth is out of the office . Message-ID: I will be out of the office starting Fri 01/25/2008 and will not return until Mon 02/11/2008. From rjbuesa <@t> yahoo.com Wed Feb 6 15:18:35 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 6 15:18:39 2008 Subject: [Histonet] Tissue processor info requested In-Reply-To: Message-ID: <183846.61503.qm@web61213.mail.yahoo.com> Try to buy the small (100 cassettes) model of VIP by Sakura. Very flexible programs and outstanding long term reliability, what you can call a "real workhorse". Ren? J. Mary L Giebel/FS/VCU wrote: I would appreciate your thoughts and advice on tissue processors, both conventional and microwave. I have just been notified that I may be able to purchase a tissue processor...and they would like the quote by tomorrow morning. My previous research on processors is now outdated. I run a small research histology core with a low volume and a low budget. I mainly work with mouse tissue (heart and lungs). Most of the tissue that I work with is used for IHC, which is why I am somewhat hesitant to go with a microwave processor. I have found a unit that looks interesting, the Microm STP-120, but I am concerned because it uses a centrifugal cycle. That would not be a problem with the hearts, but the lungs that I work with are inflated. Has anyone had a problem with the unit? Any and all help would be greatly appreciated. Thank you. Regards, Mary Lee Giebel _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From szhang101 <@t> hotmail.com Wed Feb 6 15:32:01 2008 From: szhang101 <@t> hotmail.com (Shengwen Zhang) Date: Wed Feb 6 15:32:31 2008 Subject: [Histonet] Postdoc job at Stanford University - Sleep and metabolism in mice Message-ID: Hi All, This might be a stretch. But we really need to get a postdoc quickly and I would try here. Please send the information to your friends and colleagues. Thanks! Shengwen Zhang, Ph.D. Center for Narcolepsy Stanford University School of Medicine Department of Psychiatry and Behavioral Sciences 701B Welch Road, #117 Palo Alto, CA 94304-5742 Phone: 650-723-5870 Fax: 650-725-4913 ================================================================= Description Postdoctoral position in the area of sleep, synaptic plasticity and metabolism. We are seeking a motivated scientist to join the team working on sleep, hypocretin and neurosciences. For further information about the Center for Narcolepsy, please visit http://med.stanford.edu/school/Psychiatry/narcolepsy. Responsibilities * Mouse and rat handling, husbandry, breeding and genotyping of transgenics (genomic DNA preparation and PCR) * In vivo studies including surgery (transmitter and EEG/EMG implantation), drug administration, sample harvesting, documentation, data acquisition, analysis and interpretation * Molecular, biochemical, histological and immunological assays Qualifications * PhD degree in biology,neuroscience or related field * Good animal handling skills and data management skills required * Previous surgical experiences highly desirable * Experienced with instrumentation (mechanical and electronic) * Sophisticated with Microsoft Excel, statistical and graphic software * Independence in the conception and design of experiments advantageous * Ability to learn new skills quickly * Responsible and reliable From kerry.l.crabb <@t> gsk.com Wed Feb 6 16:28:58 2008 From: kerry.l.crabb <@t> gsk.com (kerry.l.crabb@gsk.com) Date: Wed Feb 6 16:29:42 2008 Subject: [Histonet] Xylene Resistant Floors Message-ID: I've had a inquiry from another lab that is planning some remodeling. They're asking if there are any xylene resistant flooring that can be put down. Other can sealed concrete does anything exist? Kerry Crabb From BMolinari <@t> heart.thi.tmc.edu Thu Feb 7 05:45:34 2008 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Thu Feb 7 05:45:35 2008 Subject: [Histonet] OT: the weather In-Reply-To: References: Message-ID: Wow! Looks more like Colorado or Wyoming! Betsy Molinari HT(ASCP) Cardiovascular Pathology Texas Heart Institute 6770 Bertner Ave. MC 1-283 Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Van Patten Sent: Wednesday, February 06, 2008 11:02 AM To: Jackie M O'Connor; kemlo Cc: histonet@lists.utsouthwestern.edu; Ingles Claire Subject: RE: [Histonet] OT: the weather Strange weather across the country! Even Maui and the "Big Island" got snow 1 week ago... http://starbulletin.com/2008/01/30/news/story01.html -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Wednesday, February 06, 2008 10:38 AM To: kemlo Cc: histonet@lists.utsouthwestern.edu; 'Ingles Claire' Subject: RE: [Histonet] OT: the weather Up here in Illinois near the Wisconsin border - we're expecting 12-14 inches of snow, and most of the staff have been allowed to go home. I just read that Hawaii has had the worst rainfall in decades, 12" of rain in 24 hours in Hilo - still not enough to extinguish the volcano, tho. (Yes, I am kidding about the volcano). "kemlo" Sent by: histonet-bounces@lists.utsouthwestern.edu 02/06/2008 10:26 AM To "'Ingles Claire'" cc histonet@lists.utsouthwestern.edu Subject RE: [Histonet] OT: the weather We across the pond are constructing an Ark; there's enough rain to float it and enough wind to give it the kinetic energy it requires. I blame...... The no smoking policy. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: 06 February 2008 15:06 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OT: the weather Hey guys: I don't know about you, but I'm really beginning to wonder about the weather. Anyway- everyone in the southern states, especially Arkansas, still there? I gripe about the snow this year, but geez. How bad was the damage from the tornadoes down in that area. Just thinking of everyone, and hoping all is well with everyone. Stay warm/dry/unburnt/etc. You bunch from across the pond can send us your extra show shovels and inflatable rafts. Anywhere you could put us up if it gets too bad over here? :) Claire _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Thu Feb 7 08:07:51 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Feb 7 08:07:58 2008 Subject: [Histonet] Xylene Resistant Floors In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A370D@IS-E2K3.grhs.net> Why would you be worried about Xylene resistant floors - unless you are spelling a lot of xylene on the floor often? Then I would be worried about my xylene exposure and my ventilation system. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kerry.l.crabb@gsk.com Sent: Wednesday, February 06, 2008 4:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Resistant Floors I've had a inquiry from another lab that is planning some remodeling. They're asking if there are any xylene resistant flooring that can be put down. Other can sealed concrete does anything exist? Kerry Crabb _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ROrr <@t> enh.org Thu Feb 7 08:34:51 2008 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Thu Feb 7 08:34:59 2008 Subject: [Histonet] PTH on clinical cases Message-ID: Hi Everyone, A clinician has asked that we run PTH on one of his patients. It's ordered so rarely, I don't even keep it in stock. I'd appreciate any recommendations for the antibody clone that is more commonly used in a clinical setting. Thanks for all of your help. Becky Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 From MMargiotta <@t> bmhmc.org Thu Feb 7 09:51:35 2008 From: MMargiotta <@t> bmhmc.org (Margiotta, Michele) Date: Thu Feb 7 09:51:43 2008 Subject: [Histonet] cracks in paraffin Message-ID: <922CE5B88F398948B4076A9A4340E7AF036AF62B@bmh_exchange.bmhmc.org> Hi All, I also have a similar ? about cracks in paraffin blocks. We are demoing a new embedding center and have been getting cracks in our blocks. Unfortunately, the cold plate temp can't be adjusted and is supposedly set at -5C. Any other ideas how to prevent this from happening? Thanks! Michele Margiotta BMHMC Histology Supervisor 631-654-7192 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. From gvdobbin <@t> ihis.org Thu Feb 7 10:02:51 2008 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Thu Feb 7 10:03:15 2008 Subject: [Histonet] cracks in paraffin Message-ID: If you are demo'ing the instrument, get the company rep involved! The last thing they want is for you to be having problems with the equipment they are hoping to sell you! Cheers! Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 There is some merit in doing the right thing rather badly, but absolutely none in doing the wrong thing excellently! >>> "Margiotta, Michele" 2/7/2008 11:51 AM >>> Hi All, I also have a similar ? about cracks in paraffin blocks. We are demoing a new embedding center and have been getting cracks in our blocks. Unfortunately, the cold plate temp can't be adjusted and is supposedly set at -5C. Any other ideas how to prevent this from happening? Thanks! Michele Margiotta BMHMC Histology Supervisor 631-654-7192 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ayant pour objet une personne ou un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer compl?tement de votre syst?me informatique. From jqb7 <@t> CDC.GOV Thu Feb 7 10:08:37 2008 From: jqb7 <@t> CDC.GOV (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu Feb 7 10:12:05 2008 Subject: [Histonet] cracks in paraffin In-Reply-To: <922CE5B88F398948B4076A9A4340E7AF036AF62B@bmh_exchange.bmhmc.org> References: <922CE5B88F398948B4076A9A4340E7AF036AF62B@bmh_exchange.bmhmc.org> Message-ID: <34BB307EFC9A65429BBB49E330675F72045E24F8@LTA3VS003.ees.hhs.gov> What kind of embedding center doesn't allow for a range in cooling temps? Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margiotta, Michele Sent: Thursday, February 07, 2008 10:52 AM To: histonet@pathology.swmed.edu Subject: [Histonet] cracks in paraffin Hi All, I also have a similar ? about cracks in paraffin blocks. We are demoing a new embedding center and have been getting cracks in our blocks. Unfortunately, the cold plate temp can't be adjusted and is supposedly set at -5C. Any other ideas how to prevent this from happening? Thanks! Michele Margiotta BMHMC Histology Supervisor 631-654-7192 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Feb 7 10:18:02 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 7 10:18:12 2008 Subject: [Histonet] cracks in paraffin In-Reply-To: <922CE5B88F398948B4076A9A4340E7AF036AF62B@bmh_exchange.bmhmc.org> Message-ID: <926212.84586.qm@web61218.mail.yahoo.com> If you have an embedding center demo that cannot control the cold plate temperature, get another manufacturer. It would not be wise to buy something that even from the start cannot do what you need and is causing problems. Contact another rep with this problem and get another demo from another maker. This is a problem you can eliminate early. Ren? J. "Margiotta, Michele" wrote: Hi All, I also have a similar ? about cracks in paraffin blocks. We are demoing a new embedding center and have been getting cracks in our blocks. Unfortunately, the cold plate temp can't be adjusted and is supposedly set at -5C. Any other ideas how to prevent this from happening? Thanks! Michele Margiotta BMHMC Histology Supervisor 631-654-7192 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From POWELL_SA <@t> Mercer.edu Thu Feb 7 11:29:55 2008 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Thu Feb 7 11:33:59 2008 Subject: [Histonet] Region III/GSH Symposium Message-ID: <01MR0JI3A9SG001ZDF@Macon2.Mercer.edu> The Georgia Society for Histotechnology proudly hosts the NSH Region III Meeting. It will be held April 3-5 2008 at the Westin Peachtree Plaza in Atlanta, GA Please make your Reservations Now by calling 404-659-1400. Deadline for reservations is March 3rd so please do not wait. Room rate: $129 single, double, triple, quad plus taxes. See the tentative program below. The final printed programs will be mailed next week and will also be posted on the GSH web site next week. Got to www.histosearch.com/gsh for the final program and registration form. Thanks, Thursday Evening: Vendor Reception Friday: April 4, 2008 Dr. John McCormick - Seventeenth, Eighteenth, & Nineteenth Century Studies of Nature w/ Microscope Technique Jim Sanzo, Nikon - Confocal Microscopy Dr. Sherif Zaki, CDC - Infectious Disease Jonathan Jones, GaDermpath - Ethics in Histology Connie Wavrin, Atlanta VA Med Centers - Microwave Processing Joe Myers, Biocare - Workshop #1 Antigen Retrieval and Double Immunostaining Debra Flynn and Mike Reichenbach, Ventana -Workshop #2 CSI IHC Faith Gorousingh, Atlanta VA Med Centers - Workshop #3 Special Stains Saturday: April 5, 2008 Dr. Josh Lane, Lane Dermatology - Mohs Histology from a Pathologist Viewpoint Shirley Powell, Mercer - Macro Sectioning Russell Long, Nikon, - The New and Improved Microscope Speaker TBA, Leica - Xylene free Processing Connie Wavrin, Atlanta VA Med Centers - Workshop # 4 Grossing Techniques Speaker TBA, Leica - Workshop #5 Immunofluorescence From detmar <@t> mshri.on.ca Thu Feb 7 11:49:48 2008 From: detmar <@t> mshri.on.ca (Jacqui Detmar) Date: Thu Feb 7 11:50:07 2008 Subject: [Histonet] IL1 KO mouse and cytokines in neurons In-Reply-To: <020420082239.9958.47A7941A000DA756000026E622007354469D09020704040A0105@comcast.net> References: <020420082239.9958.47A7941A000DA756000026E622007354469D09020704040A0105@comcast.net> Message-ID: Hey there. Yup, I agree that using KO mice as controls can be a problem, although I work with about a dozen different KOs and have had no problems, so I think it's a matter of knowing *what exactly* has been knocked out, plus keeping your fingers crossed and praying to the right gods . Anyway, if you're worried about cytokine signaling to adjacent cells (which was great thinking, Ray!), perhaps another approach is to use either caspase-1 KO (mice available commercially) or caspase-11 KO mice. These enzymes process pro-IL-1alpha and beta to their active, secreted forms. Thus, the pro forms should be retained within the primary cell types synthesizing the cytokines. Both caspase-11 and caspase-1 KO mice have been reported to exhibit very low levels of secreted, processed IL-1beta and IL-1alpha. I have not yet seen anyone produce double caspase-1/caspase-11 KOs. Jacqui Detmar, Post-doctoral Fellow Samuel Lunenfeld Research Institute, room 876 Mount Sinai Hospital 600 University Avenue Toronto, ON, Canada M5G 1X5 phone: 416-586-4800 x2451/x2290 fax: 416-586-8588 email: detmar@mshri.on.ca ________________________________ From: koellingr@comcast.net [mailto:koellingr@comcast.net] Sent: Monday, February 04, 2008 5:39 PM To: Jacqui Detmar; Leyva-Grado, Victor; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IL1 KO mouse and cytokines in neurons Victor and Jacqui, Could "some" neurons appearing to be immunoreactive to TNF alpha or Il-1 beta simply be the fact that they are cytokines? Small molecular weight, they are meant to be released from cells and not membrane bound there or as part of the cell structure. Glial cells, by the very fact of what they are, are intimately associated with neurons. How was tissue fixed? Maybe they are dispersed or leaking to a neighboring neuron. Many targets are anchored where they are, such as your NeuN label, and that target is not going anywhere. Cytokines do. The references of 10-15 years ago were novel then. Not so now. TNK K/o as you said you can get. At Wash State, you should have a good mouse transgenics and mouse k/o husbandry facility. With some Balb/c's, they should be able to generate IL-1 alpha -/-. beta -/- or alpha/beta double knock/outs. Not that difficult for a good knock out genetics lab. If you are using the&n bsp;knock outs as control for the IHC staining (and this applies to any use of knock-outs as negative controls for any IHC staining) be sure you know EXACTLY what is knocked out. A mouse frizzle/frazzle knock-out does not imply the total absence of mouse frizzle/frazzel gene and protein. One particular coding exon in a multi-exon gene could leave the protein unresponsive or unusable in-vivo or shortened but enough might be left, and if the proper epitope is left, for IHC staining to occur. Knock out is not the same as complete absence. So you can stain something that is "knocked out" and that is why you need to be aware of EXACTLY what is knocked out. Many people, including yours truely, has been caught like this. Ray From sotlak <@t> yahoo.gr Thu Feb 7 12:42:52 2008 From: sotlak <@t> yahoo.gr (sotiris lakis) Date: Thu Feb 7 12:42:58 2008 Subject: [Histonet] (no subject) Message-ID: <39159.79814.qm@web23012.mail.ird.yahoo.com> hello everyone, I am interested in performing immunohistochemistry for electron microscopy. I have a really general idea about it and I lack specific information about the protocol. Is their any site on the net where I could start from? Can commercially availlable antibodies for IHC be used for immunogold too? thanks Sotiris Lakis Thessaloniki, Hellas --------------------------------- ?????????????? Yahoo! ?????????? ?? ?????????? ???? ???? (spam); ?? Yahoo! Mail ???????? ??? ???????? ?????? ????????? ???? ??? ??????????? ????????? http://login.yahoo.com/config/mail?.intl=gr From koellingr <@t> comcast.net Thu Feb 7 12:47:06 2008 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Thu Feb 7 12:47:14 2008 Subject: [Histonet] IL1 KO mouse and cytokines in neurons Message-ID: <020720081847.11317.47AB5229000C8F2A00002C3522007637049D09020704040A0105@comcast.net> Jacqui's suggestion seems super. Localizing something that is not being (or rarely) secreted seems logically to have less associated problems than localizing something that by its very nature is mobile and you are trying to discriminate it from a cell right next to it. Congratulations on your KO/IHC luck. Wish I had had some of that luck in a former life. Shows the need for collaboration and understanding between IHC types and molecular research types. "This is a -/- knockout to (x)" is insufficient communication to be assured that (x) won't be staining and costing weeks of frustration and excess work and needless work. Ray Koelling Phenopath Labs Seattle, WA -------------- Original message ---------------------- From: "Jacqui Detmar" > Hey there. Yup, I agree that using KO mice as controls can be a > problem, although I work with about a dozen different KOs and have had > no problems, so I think it's a matter of knowing *what exactly* has been > knocked out, plus keeping your fingers crossed and praying to the right > gods . Anyway, if you're worried about cytokine signaling to > adjacent cells (which was great thinking, Ray!), perhaps another > approach is to use either caspase-1 KO (mice available commercially) or > caspase-11 KO mice. These enzymes process pro-IL-1alpha and beta to > their active, secreted forms. Thus, the pro forms should be retained > within the primary cell types synthesizing the cytokines. Both > caspase-11 and caspase-1 KO mice have been reported to exhibit very low > levels of secreted, processed IL-1beta and IL-1alpha. I have not yet > seen anyone produce double caspase-1/caspase-11 KOs. > > > > Jacqui Detmar, Post-doctoral Fellow > > Samuel Lunenfeld Research Institute, room 876 > > Mount Sinai Hospital > > 600 University Avenue > > Toronto, ON, Canada > > M5G 1X5 > > > > phone: 416-586-4800 x2451/x2290 > > fax: 416-586-8588 > > email: detmar@mshri.on.ca > > > > ________________________________ > > From: koellingr@comcast.net [mailto:koellingr@comcast.net] > Sent: Monday, February 04, 2008 5:39 PM > To: Jacqui Detmar; Leyva-Grado, Victor; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] IL1 KO mouse and cytokines in neurons > > > > Victor and Jacqui, > > > > Could "some" neurons appearing to be immunoreactive to TNF alpha or Il-1 > beta simply be the fact that they are cytokines? Small molecular > weight, they are meant to be released from cells and not membrane bound > there or as part of the cell structure. Glial cells, by the very fact > of what they are, are intimately associated with neurons. How was > tissue fixed? Maybe they are dispersed or leaking to a neighboring > neuron. Many targets are anchored where they are, such as your NeuN > label, and that target is not going anywhere. Cytokines do. The > references of 10-15 years ago were novel then. Not so now. TNK K/o as > you said you can get. At Wash State, you should have a good mouse > transgenics and mouse k/o husbandry facility. With some Balb/c's, they > should be able to generate IL-1 alpha -/-. beta -/- or alpha/beta double > knock/outs. Not that difficult for a good knock out genetics lab. If > you are using the&n bsp;knock outs as control for the IHC staining (and > this applies to any use of knock-outs as negative controls for any IHC > staining) be sure you know EXACTLY what is knocked out. A mouse > frizzle/frazzle knock-out does not imply the total absence of mouse > frizzle/frazzel gene and protein. One particular coding exon in a > multi-exon gene could leave the protein unresponsive or unusable in-vivo > or shortened but enough might be left, and if the proper epitope is > left, for IHC staining to occur. Knock out is not the same as complete > absence. So you can stain something that is "knocked out" and that is > why you need to be aware of EXACTLY what is knocked out. Many people, > including yours truely, has been caught like this. > > Ray > > > > > > From carl.hobbs <@t> kcl.ac.uk Thu Feb 7 13:00:58 2008 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Thu Feb 7 13:01:26 2008 Subject: [Histonet] Tissue separating from the wax when floating out Message-ID: <000301c869bb$c6dd4590$4001a8c0@carlba65530bda> A colleague has brought his pwax blocks of brain tissue from his lab and is using my pwax section cutting system to cut his blocks: after floating them out on the waterbath ( fresh distilled water, maintained at 40C- yes, we've tried lower temps and the sections either just don't flatten out or still separate, after a longer period), they separate away from the wax, initially along the line of the meninges separating the two areas of brain, in coronal section. I have looked at his blocks and they seem well-processed - no fracturing/white areas, a good way to tell is to look at the cut blocks a week after sectioning to see if the face of the tissue has shrunk down into the block, no they haven't. I have cut some myself: same problem. I took one of these blocks and re-immersed it in fresh molten wax for 2hrs ( I tried simple paraffin wax and then Paraplast Plus)....no improvement. The blocks serial-section beautifully. He has asked his lab if they have this problem and they do not. He has not seen it before, either, when cutting in his own lab. This happens to me also, with the occassional block. Has anyone come across this problem? Why does it occur? ( my processing details: 2hrs in each of 30, 70, 95, 100, 100% IMS, IMS:Xylene 1:1, xylene, xylene, wax, wax...then 1hr in embedding station wax bath. On a Leica processing machine, with agitation. Embedding is swiftly carried out, so minimal cooling of wax. Be most grateful for any reasoning. Best wishes carl From vic <@t> vetmed.wsu.edu Thu Feb 7 13:17:13 2008 From: vic <@t> vetmed.wsu.edu (Leyva-Grado, Victor) Date: Thu Feb 7 13:17:23 2008 Subject: [Histonet] IL1 KO mouse and cytokines in neurons In-Reply-To: <020720081847.11317.47AB5229000C8F2A00002C3522007637049D09020704040A0105@comcast.net> References: <020720081847.11317.47AB5229000C8F2A00002C3522007637049D09020704040A0105@comcast.net> Message-ID: <34EFB08480241347BEBE1F095ABB96F420783E@cvm36.vetmed.wsu.edu> Dears Jacqui and Ray, I just want to thank you for all your thoughtful insights and the great ideas regarding my quest within the IHC world. I will let you know what happened and hopefully you can read my published results later (rather sooner than later). I just have to agree with Ray regarding the need for collaboration between these 2 areas that seem (or we make them look like) to be very different. Muchas gracias, Victor Leyva -----Original Message----- From: koellingr@comcast.net [mailto:koellingr@comcast.net] Sent: Thursday, February 07, 2008 10:47 AM To: Jacqui Detmar; Leyva-Grado, Victor; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IL1 KO mouse and cytokines in neurons Jacqui's suggestion seems super. Localizing something that is not being (or rarely) secreted seems logically to have less associated problems than localizing something that by its very nature is mobile and you are trying to discriminate it from a cell right next to it. Congratulations on your KO/IHC luck. Wish I had had some of that luck in a former life. Shows the need for collaboration and understanding between IHC types and molecular research types. "This is a -/- knockout to (x)" is insufficient communication to be assured that (x) won't be staining and costing weeks of frustration and excess work and needless work. Ray Koelling Phenopath Labs Seattle, WA -------------- Original message ---------------------- From: "Jacqui Detmar" > Hey there. Yup, I agree that using KO mice as controls can be a > problem, although I work with about a dozen different KOs and have had > no problems, so I think it's a matter of knowing *what exactly* has > been knocked out, plus keeping your fingers crossed and praying to the > right gods . Anyway, if you're worried about cytokine signaling > to adjacent cells (which was great thinking, Ray!), perhaps another > approach is to use either caspase-1 KO (mice available commercially) > or > caspase-11 KO mice. These enzymes process pro-IL-1alpha and beta to > their active, secreted forms. Thus, the pro forms should be retained > within the primary cell types synthesizing the cytokines. Both > caspase-11 and caspase-1 KO mice have been reported to exhibit very > low levels of secreted, processed IL-1beta and IL-1alpha. I have not > yet seen anyone produce double caspase-1/caspase-11 KOs. > > > > Jacqui Detmar, Post-doctoral Fellow > > Samuel Lunenfeld Research Institute, room 876 > > Mount Sinai Hospital > > 600 University Avenue > > Toronto, ON, Canada > > M5G 1X5 > > > > phone: 416-586-4800 x2451/x2290 > > fax: 416-586-8588 > > email: detmar@mshri.on.ca > > > > ________________________________ > > From: koellingr@comcast.net [mailto:koellingr@comcast.net] > Sent: Monday, February 04, 2008 5:39 PM > To: Jacqui Detmar; Leyva-Grado, Victor; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] IL1 KO mouse and cytokines in neurons > > > > Victor and Jacqui, > > > > Could "some" neurons appearing to be immunoreactive to TNF alpha or > Il-1 beta simply be the fact that they are cytokines? Small molecular > weight, they are meant to be released from cells and not membrane > bound there or as part of the cell structure. Glial cells, by the > very fact of what they are, are intimately associated with neurons. > How was tissue fixed? Maybe they are dispersed or leaking to a > neighboring neuron. Many targets are anchored where they are, such as > your NeuN label, and that target is not going anywhere. Cytokines do. > The references of 10-15 years ago were novel then. Not so now. TNK > K/o as you said you can get. At Wash State, you should have a good > mouse transgenics and mouse k/o husbandry facility. With some > Balb/c's, they should be able to generate IL-1 alpha -/-. beta -/- or > alpha/beta double knock/outs. Not that difficult for a good knock out > genetics lab. If you are using the&n bsp;knock outs as control for > the IHC staining (and this applies to any use of knock-outs as > negative controls for any IHC > staining) be sure you know EXACTLY what is knocked out. A mouse > frizzle/frazzle knock-out does not imply the total absence of mouse > frizzle/frazzel gene and protein. One particular coding exon in a > multi-exon gene could leave the protein unresponsive or unusable > in-vivo or shortened but enough might be left, and if the proper > epitope is left, for IHC staining to occur. Knock out is not the same > as complete absence. So you can stain something that is "knocked out" > and that is why you need to be aware of EXACTLY what is knocked out. > Many people, including yours truely, has been caught like this. > > Ray > > > > > > From rjbuesa <@t> yahoo.com Thu Feb 7 13:35:57 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 7 13:36:06 2008 Subject: [Histonet] Tissue separating from the wax when floating out In-Reply-To: <000301c869bb$c6dd4590$4001a8c0@carlba65530bda> Message-ID: <359488.10380.qm@web61218.mail.yahoo.com> You have pretty much covered all the possible problems, so it is difficult to have an idea about the causes of the problem. If I understood you well those blocks cut OK in your friend's labs, but not in yours? Then what is that you do differently while sectioning that is not done in your friend's lab? That would be a start. Regardless of wht you think this seems to be an infiltration problem or a poor adherence between the paraffin used in the last bath and the one used in the embedding center. Even if you put the blocks in melted paraffn x2 hours, how was the last paraffin in the tissue processor?. Do you have to take serial sections? Can you just take one section at a time leaving them less time in the water bath? Again, this is typical behaviour when the tissue is not well infiltrated. Try a cold water bath and add liquid detergent to it (0.20-0.25 mL) to allow the sections to expand even at low temperature, perhaps you will be able to prevent the paraffin to separate from the tissue. Ren? J. Carl Hobbs wrote: A colleague has brought his pwax blocks of brain tissue from his lab and is using my pwax section cutting system to cut his blocks: after floating them out on the waterbath ( fresh distilled water, maintained at 40C- yes, we've tried lower temps and the sections either just don't flatten out or still separate, after a longer period), they separate away from the wax, initially along the line of the meninges separating the two areas of brain, in coronal section. I have looked at his blocks and they seem well-processed - no fracturing/white areas, a good way to tell is to look at the cut blocks a week after sectioning to see if the face of the tissue has shrunk down into the block, no they haven't. I have cut some myself: same problem. I took one of these blocks and re-immersed it in fresh molten wax for 2hrs ( I tried simple paraffin wax and then Paraplast Plus)....no improvement. The blocks serial-section beautifully. He has asked his lab if they have this problem and they do not. He has not seen it before, either, when cutting in his own lab. This happens to me also, with the occassional block. Has anyone come across this problem? Why does it occur? ( my processing details: 2hrs in each of 30, 70, 95, 100, 100% IMS, IMS:Xylene 1:1, xylene, xylene, wax, wax...then 1hr in embedding station wax bath. On a Leica processing machine, with agitation. Embedding is swiftly carried out, so minimal cooling of wax. Be most grateful for any reasoning. Best wishes carl _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From gayle.callis <@t> bresnan.net Thu Feb 7 13:47:24 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Thu Feb 7 13:47:30 2008 Subject: [Histonet] cracks in paraffin References: <922CE5B88F398948B4076A9A4340E7AF036AF62B@bmh_exchange.bmhmc.org> Message-ID: <002a01c869c2$42e401f0$6501a8c0@Sunney> I suggest demo'ing one that has temperature adjustment. We have a Sakura Finetek embedding center that allows temperature adjustment for the cold module block cooling, and all heated areas. Gayle M. Callis HT/HTL/MT(ASCP) Bozeman MT 59715 ----- Original Message ----- From: "Margiotta, Michele" To: Sent: Thursday, February 07, 2008 8:51 AM Subject: [Histonet] cracks in paraffin Hi All, I also have a similar ? about cracks in paraffin blocks. We are demoing a new embedding center and have been getting cracks in our blocks. Unfortunately, the cold plate temp can't be adjusted and is supposedly set at -5C. Any other ideas how to prevent this from happening? Thanks! Michele Margiotta BMHMC Histology Supervisor 631-654-7192 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Shirley_PHUA <@t> hsa.gov.sg Thu Feb 7 14:02:50 2008 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Thu Feb 7 14:03:13 2008 Subject: [Histonet] Shirley Phua is out-of-office ... Message-ID: I will be out of the office from 08-02-2008 to 15-02-2008. I'll be overseas 08-16 January 2008. Pathologists: I will process your requests when I return. If urgent, please forward your email to Henry_Kyaw@hsa.gov.sg From Sharon.Genest <@t> saskatoonhealthregion.ca Thu Feb 7 13:38:24 2008 From: Sharon.Genest <@t> saskatoonhealthregion.ca (Genest, Sharon SktnHR) Date: Thu Feb 7 15:38:27 2008 Subject: [Histonet] Gomori Aldehyde Fuchsin and Control Blocks Message-ID: <1C152FCB03A4F84197818DEE847F0D4A038F3D16@stampy.sktnhr.ca> I am having difficulty obtaining paraldehyde for this method does anyone have suggestions for and alternate method that does not use this? I already have Movat and Verhoeff but some Pathologists prefer the GAF. Does anyone have a supplier with a Canadian distributor that I can purchase control blocks for amoebae, spirochetes, micobacterium leprae? Sharon Genest MLT Acting Technologist III Histology Saskatoon Health Region Phone: (306)655-8197 Email: sharon.genest@saskatoonhealthregion.ca From jnocito <@t> satx.rr.com Thu Feb 7 20:04:24 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Feb 7 20:04:11 2008 Subject: [Histonet] OT: the weather References: Message-ID: <001201c869f6$ed9eae90$0302a8c0@yourxhtr8hvc4p> so much for global warning ----- Original Message ----- From: "Molinari, Betsy" To: Sent: Thursday, February 07, 2008 5:45 AM Subject: RE: [Histonet] OT: the weather Wow! Looks more like Colorado or Wyoming! Betsy Molinari HT(ASCP) Cardiovascular Pathology Texas Heart Institute 6770 Bertner Ave. MC 1-283 Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Van Patten Sent: Wednesday, February 06, 2008 11:02 AM To: Jackie M O'Connor; kemlo Cc: histonet@lists.utsouthwestern.edu; Ingles Claire Subject: RE: [Histonet] OT: the weather Strange weather across the country! Even Maui and the "Big Island" got snow 1 week ago... http://starbulletin.com/2008/01/30/news/story01.html -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Wednesday, February 06, 2008 10:38 AM To: kemlo Cc: histonet@lists.utsouthwestern.edu; 'Ingles Claire' Subject: RE: [Histonet] OT: the weather Up here in Illinois near the Wisconsin border - we're expecting 12-14 inches of snow, and most of the staff have been allowed to go home. I just read that Hawaii has had the worst rainfall in decades, 12" of rain in 24 hours in Hilo - still not enough to extinguish the volcano, tho. (Yes, I am kidding about the volcano). "kemlo" Sent by: histonet-bounces@lists.utsouthwestern.edu 02/06/2008 10:26 AM To "'Ingles Claire'" cc histonet@lists.utsouthwestern.edu Subject RE: [Histonet] OT: the weather We across the pond are constructing an Ark; there's enough rain to float it and enough wind to give it the kinetic energy it requires. I blame...... The no smoking policy. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: 06 February 2008 15:06 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OT: the weather Hey guys: I don't know about you, but I'm really beginning to wonder about the weather. Anyway- everyone in the southern states, especially Arkansas, still there? I gripe about the snow this year, but geez. How bad was the damage from the tornadoes down in that area. Just thinking of everyone, and hoping all is well with everyone. Stay warm/dry/unburnt/etc. You bunch from across the pond can send us your extra show shovels and inflatable rafts. Anywhere you could put us up if it gets too bad over here? :) Claire _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From drkwolfe <@t> telus.net Thu Feb 7 23:25:08 2008 From: drkwolfe <@t> telus.net (Joseph Kapler) Date: Thu Feb 7 23:25:17 2008 Subject: [Histonet] OT: the weather In-Reply-To: <001201c869f6$ed9eae90$0302a8c0@yourxhtr8hvc4p> References: <001201c869f6$ed9eae90$0302a8c0@yourxhtr8hvc4p> Message-ID: <000301c86a12$f8698680$e93c9380$@net> Alberta Canada, we had a week of -30 to -40 C, with wind chills taking the temp down to the -50's and -60's. Definitely time to reconsider moving back home to someplace warm ... Early retirement... then nothing but sun and sand... Our prayers go out to those that were affected by the tornados down south, and to our Eastern Canada neighbours who have been hit by one of the nastier storms this decade. Winter Sucks! ... Bring on the Hot Sunny Days again ... please... Joe -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, February 07, 2008 7:04 PM To: Molinari, Betsy; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] OT: the weather so much for global warning ----- Original Message ----- From: "Molinari, Betsy" To: Sent: Thursday, February 07, 2008 5:45 AM Subject: RE: [Histonet] OT: the weather Wow! Looks more like Colorado or Wyoming! Betsy Molinari HT(ASCP) Cardiovascular Pathology Texas Heart Institute 6770 Bertner Ave. MC 1-283 Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Van Patten Sent: Wednesday, February 06, 2008 11:02 AM To: Jackie M O'Connor; kemlo Cc: histonet@lists.utsouthwestern.edu; Ingles Claire Subject: RE: [Histonet] OT: the weather Strange weather across the country! Even Maui and the "Big Island" got snow 1 week ago... http://starbulletin.com/2008/01/30/news/story01.html -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Wednesday, February 06, 2008 10:38 AM To: kemlo Cc: histonet@lists.utsouthwestern.edu; 'Ingles Claire' Subject: RE: [Histonet] OT: the weather Up here in Illinois near the Wisconsin border - we're expecting 12-14 inches of snow, and most of the staff have been allowed to go home. I just read that Hawaii has had the worst rainfall in decades, 12" of rain in 24 hours in Hilo - still not enough to extinguish the volcano, tho. (Yes, I am kidding about the volcano). "kemlo" Sent by: histonet-bounces@lists.utsouthwestern.edu 02/06/2008 10:26 AM To "'Ingles Claire'" cc histonet@lists.utsouthwestern.edu Subject RE: [Histonet] OT: the weather We across the pond are constructing an Ark; there's enough rain to float it and enough wind to give it the kinetic energy it requires. I blame...... The no smoking policy. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: 06 February 2008 15:06 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OT: the weather Hey guys: I don't know about you, but I'm really beginning to wonder about the weather. Anyway- everyone in the southern states, especially Arkansas, still there? I gripe about the snow this year, but geez. How bad was the damage from the tornadoes down in that area. Just thinking of everyone, and hoping all is well with everyone. Stay warm/dry/unburnt/etc. You bunch from across the pond can send us your extra show shovels and inflatable rafts. Anywhere you could put us up if it gets too bad over here? :) Claire _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rydomsal <@t> yahoo.com Fri Feb 8 02:58:20 2008 From: rydomsal <@t> yahoo.com (Ryan Dominique Salazar) Date: Fri Feb 8 02:58:25 2008 Subject: [Histonet] brain tissue sections Message-ID: <822343.33604.qm@web52309.mail.re2.yahoo.com> Hi, Please help me with my brain tissue sections, I'm having difficulties in staining them because they disintegrate during H&E staining, resulting into folded and incomplete sections into the slide. I processed the tissues 2 days after fixation using Leica ASP300S (all new reagents). I have no problem in cutting 5 u thickness during microtomy. I used adhesive pre-treated slides and Milli-Q water during orientation and fishing out in the floatation bath. I use flattening table as hot plate and heat the freshly cut slides at 62C, for not less than 10 minutes. During staining, I use 3 changes of xylene and abs. ethanol followed by 80% then 70% ethanol, all of them for 3 minutes. I used milli-Q water for hydration and washing during staining. For differentiation and bluing, I use 1% acid alcohol and ammonia water. Can you also provide me some techniques and difficulties for Perl's prussian blue and amyloid staining. Thank you very much for your help and your website which changes the lives of ordinary histologist like me. More power and God bless! --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From JWEEMS <@t> sjha.org Fri Feb 8 05:13:10 2008 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Feb 8 05:14:47 2008 Subject: [Histonet] brain tissue sections References: <822343.33604.qm@web52309.mail.re2.yahoo.com> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3204726C28@sjhaexc02.sjha.org> I would air dry the slides overnight before heating them at all. Then dry at 60C for 30 min and stain as usual. Somehow the air drying makes a difference. Good luck! j Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Ryan Dominique Salazar Sent: Fri 2/8/2008 3:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] brain tissue sections Hi, Please help me with my brain tissue sections, I'm having difficulties in staining them because they disintegrate during H&E staining, resulting into folded and incomplete sections into the slide. I processed the tissues 2 days after fixation using Leica ASP300S (all new reagents). I have no problem in cutting 5 u thickness during microtomy. I used adhesive pre-treated slides and Milli-Q water during orientation and fishing out in the floatation bath. I use flattening table as hot plate and heat the freshly cut slides at 62C, for not less than 10 minutes. During staining, I use 3 changes of xylene and abs. ethanol followed by 80% then 70% ethanol, all of them for 3 minutes. I used milli-Q water for hydration and washing during staining. For differentiation and bluing, I use 1% acid alcohol and ammonia water. Can you also provide me some techniques and difficulties for Perl's prussian blue and amyloid staining. Thank you very much for your help and your website which changes the lives of ordinary histologist like me. More power and God bless! --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From jennifer.l.hofecker <@t> Vanderbilt.Edu Fri Feb 8 06:59:37 2008 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Fri Feb 8 06:59:40 2008 Subject: [Histonet] brain tissue sections In-Reply-To: <822343.33604.qm@web52309.mail.re2.yahoo.com> Message-ID: <898D946569A27444B65667A49C07405201564473@mailbe06.mc.vanderbilt.edu> Hello. Are you working with human brain sections? What size? The two days fixation, is that whole brains or dissected sections? I agree with the recommendation by Joyce that you should air dry the slides (make sure no water underneath sections) BEFORE placing on the hot plate. It will not only help section adherence, but will help to prevent nuclear bubbling from the water "cooking" under the section. It is entirely possible that you have a fixation problem. I've found when we rush fixation on autopsy brain sections, they may cut with no difficulty. Then as we stain (frequently in the bluing stage) they float off the adhesive slides right before my eyes! All of this may be contributory to animal work as well, but I know I've experienced it with human tissue, first hand. As for your requests for staining techniques, I'd be glad to forward our amyloid methods separately. We typically do not to Iron stains here in Neuropathology, but there are many good protocols out there for Perl's. Sorry to ask so many questions in response to yours. Feel free to contact me if I can be of further help. Have a great rest of the week. Jennifer Jennifer L. Hofecker, HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph. (615)343-0083 fax. (615)343-7089 NSH Quality Control Committee Chair -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ryan Dominique Salazar Sent: Friday, February 08, 2008 2:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] brain tissue sections Hi, Please help me with my brain tissue sections, I'm having difficulties in staining them because they disintegrate during H&E staining, resulting into folded and incomplete sections into the slide. I processed the tissues 2 days after fixation using Leica ASP300S (all new reagents). I have no problem in cutting 5 u thickness during microtomy. I used adhesive pre-treated slides and Milli-Q water during orientation and fishing out in the floatation bath. I use flattening table as hot plate and heat the freshly cut slides at 62C, for not less than 10 minutes. During staining, I use 3 changes of xylene and abs. ethanol followed by 80% then 70% ethanol, all of them for 3 minutes. I used milli-Q water for hydration and washing during staining. For differentiation and bluing, I use 1% acid alcohol and ammonia water. Can you also provide me some techniques and difficulties for Perl's prussian blue and amyloid staining. Thank you very much for your help and your website which changes the lives of ordinary histologist like me. More power and God bless! --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Feb 8 08:19:24 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 8 08:19:35 2008 Subject: [Histonet] brain tissue sections In-Reply-To: <822343.33604.qm@web52309.mail.re2.yahoo.com> Message-ID: <94735.96932.qm@web61218.mail.yahoo.com> Ryan: Brain and CNS tissues in general are very tricky to process because all the lipid contents of the white matter and the fact that it is distributed sometimes unevenly through the tissue and that tissues from different areas have different rations of white/gray matter. What you are describing is caused by poor infiltration and that has nothing to do with the instrument you use, or the "freshness" of your reagents, but with the protocol and the time the CNS tissues are left in each step. You have to have a protocol that assures the correct infiltration of the white matter to assure that it will not separate either when sectioning or staining (as is happening to you). Once you get to a blook poorly or unevenly infiltrated it is irrelevant what you try to do with the section itself. The tissue has already been poorly infiltrated and that is essentially irreversible, not matter how many anecdotal experiences to the contrary may lead you to believe otherwise. You have to develop a protocol suitable for infiltrating white matter, and this will also be suitable for the gary matter areas, with more cells and much less lipids. If at all possible discard your blocks and find a good protocol to work with. At the end will be more straight forward and will serve you for years to come and will avoid all the frustrations you are going through now. Ren? J. Ryan Dominique Salazar wrote: --------------------------------- Never miss a thing. Make Yahoo your homepage. From b-frederick <@t> northwestern.edu Fri Feb 8 08:24:02 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Feb 8 08:24:16 2008 Subject: [Histonet] OT: the weather In-Reply-To: <001201c869f6$ed9eae90$0302a8c0@yourxhtr8hvc4p> Message-ID: <000101c86a5e$43c08660$d00f7ca5@lurie.northwestern.edu> You realize we'll be told that we got all this snow because of global warming? Just because we haven't had a real Chicago winter in a while people are freaking out. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, February 07, 2008 8:04 PM To: Molinari, Betsy; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] OT: the weather so much for global warning ----- Original Message ----- From: "Molinari, Betsy" To: Sent: Thursday, February 07, 2008 5:45 AM Subject: RE: [Histonet] OT: the weather Wow! Looks more like Colorado or Wyoming! Betsy Molinari HT(ASCP) Cardiovascular Pathology Texas Heart Institute 6770 Bertner Ave. MC 1-283 Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Van Patten Sent: Wednesday, February 06, 2008 11:02 AM To: Jackie M O'Connor; kemlo Cc: histonet@lists.utsouthwestern.edu; Ingles Claire Subject: RE: [Histonet] OT: the weather Strange weather across the country! Even Maui and the "Big Island" got snow 1 week ago... http://starbulletin.com/2008/01/30/news/story01.html -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Wednesday, February 06, 2008 10:38 AM To: kemlo Cc: histonet@lists.utsouthwestern.edu; 'Ingles Claire' Subject: RE: [Histonet] OT: the weather Up here in Illinois near the Wisconsin border - we're expecting 12-14 inches of snow, and most of the staff have been allowed to go home. I just read that Hawaii has had the worst rainfall in decades, 12" of rain in 24 hours in Hilo - still not enough to extinguish the volcano, tho. (Yes, I am kidding about the volcano). "kemlo" Sent by: histonet-bounces@lists.utsouthwestern.edu 02/06/2008 10:26 AM To "'Ingles Claire'" cc histonet@lists.utsouthwestern.edu Subject RE: [Histonet] OT: the weather We across the pond are constructing an Ark; there's enough rain to float it and enough wind to give it the kinetic energy it requires. I blame...... The no smoking policy. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: 06 February 2008 15:06 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OT: the weather Hey guys: I don't know about you, but I'm really beginning to wonder about the weather. Anyway- everyone in the southern states, especially Arkansas, still there? I gripe about the snow this year, but geez. How bad was the damage from the tornadoes down in that area. Just thinking of everyone, and hoping all is well with everyone. Stay warm/dry/unburnt/etc. You bunch from across the pond can send us your extra show shovels and inflatable rafts. Anywhere you could put us up if it gets too bad over here? :) Claire _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Fri Feb 8 08:36:14 2008 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Fri Feb 8 08:36:17 2008 Subject: [Histonet] Xylene Resistant Floors In-Reply-To: Message-ID: <273699.48573.qm@web50307.mail.re2.yahoo.com> Epoxy floors will do the trick, they are pretty expensive though. They need to be "poured" and allowed to cure for a certain amount of time before they can be used. They are resistant to just about anything. Kim kerry.l.crabb@gsk.com wrote: I've had a inquiry from another lab that is planning some remodeling. They're asking if there are any xylene resistant flooring that can be put down. Other can sealed concrete does anything exist? Kerry Crabb _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Kim Merriam, MA, HT(ASCP) Cambridge, MA --------------------------------- Never miss a thing. Make Yahoo your homepage. From rjr6 <@t> psu.edu Fri Feb 8 08:58:03 2008 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Fri Feb 8 08:58:53 2008 Subject: [Histonet] Crystal mount Message-ID: We used to buy our crystal mount from Biomedia in an approximately 200 ml bottle but they no longer have it. Does any have the names of other suppliers of crystal mount especially that have it in large (ie 200ml) bottles? Thanks Roberta Horner HT/HTL Penn State University Animal Diagnostic Lab From cmmathis1 <@t> bellsouth.net Fri Feb 8 09:08:47 2008 From: cmmathis1 <@t> bellsouth.net (cmmathis1@bellsouth.net) Date: Fri Feb 8 09:08:52 2008 Subject: [Histonet] crystal mount Message-ID: <020820081508.13887.47AC707F0001C4BC0000363F22230704929B0A02D2089B9A019C04040A0DBFCE9C07089B0E03030C@att.net> I recently ordered Crystal Mount from Sigma. Product # C 0612 From MElliott <@t> mrl.ubc.ca Fri Feb 8 09:48:08 2008 From: MElliott <@t> mrl.ubc.ca (Mark Elliott) Date: Fri Feb 8 09:48:55 2008 Subject: [Histonet] Histology Grade Solvents In-Reply-To: <35379A74.751@mail.mrl.ubc.ca> References: <35379A74.751@mail.mrl.ubc.ca> Message-ID: <47AC0938.11C6.00D6.0@mrl.ubc.ca> Good Friday Morning to everyone.-YEAHHH!! It's been one of those weeks:( I was asked by someone about this and not sure what the answer is. "I have a quick question for you regarding histology grade solvents. Many companies use this as a grade but is there an official specification for this grade? If so, could you direct me to the document." Any ideas?? Thanks Mark ***CONFIDENTIALITY NOTICE*** This electronic message is intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From jennifer.harvey <@t> Vanderbilt.Edu Fri Feb 8 09:52:30 2008 From: jennifer.harvey <@t> Vanderbilt.Edu (Harvey, Jennifer Lynn) Date: Fri Feb 8 09:52:34 2008 Subject: [Histonet] Crystal mount In-Reply-To: References: Message-ID: Roberta, Have you been able to get someone to answer at Biomedia? Both my Fisher rep. and I haven't been able to get anyone to answer the phones or email for over a month. I ended up finding another source for what I needed. Is crystal mount manufactured by Biomeda? Jennifer Harvey, HT(ASCP) QIHC Vanderbilt Vision Research Center RM 8105 MCE North Tower 1215 21st Ave. South Nashville, TN 37232-8808 Phone: 615-936-1486 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Friday, February 08, 2008 8:58 AM To: HistoNet Subject: [Histonet] Crystal mount We used to buy our crystal mount from Biomedia in an approximately 200 ml bottle but they no longer have it. Does any have the names of other suppliers of crystal mount especially that have it in large (ie 200ml) bottles? Thanks Roberta Horner HT/HTL Penn State University Animal Diagnostic Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wfubmc.edu Fri Feb 8 10:23:36 2008 From: mward <@t> wfubmc.edu (Martha Ward) Date: Fri Feb 8 10:23:56 2008 Subject: [Histonet] Crystal mount In-Reply-To: References: Message-ID: <61135F0455D33347B5AAE209B903A30420C22C30@EXCHVS2.medctr.ad.wfubmc.edu> I also have not been able to contact Biomeda for over a month. I have replaced the items I was buying from them but it is very odd that they seem to have disappeared! Anybody know what is going on with them? I'm just curious. Martha Ward Wake Forest University Baptist Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Harvey, Jennifer Lynn Sent: Friday, February 08, 2008 10:53 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Crystal mount Roberta, Have you been able to get someone to answer at Biomedia? Both my Fisher rep. and I haven't been able to get anyone to answer the phones or email for over a month. I ended up finding another source for what I needed. Is crystal mount manufactured by Biomeda? Jennifer Harvey, HT(ASCP) QIHC Vanderbilt Vision Research Center RM 8105 MCE North Tower 1215 21st Ave. South Nashville, TN 37232-8808 Phone: 615-936-1486 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Friday, February 08, 2008 8:58 AM To: HistoNet Subject: [Histonet] Crystal mount We used to buy our crystal mount from Biomedia in an approximately 200 ml bottle but they no longer have it. Does any have the names of other suppliers of crystal mount especially that have it in large (ie 200ml) bottles? Thanks Roberta Horner HT/HTL Penn State University Animal Diagnostic Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Fri Feb 8 10:27:47 2008 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Feb 8 10:27:53 2008 Subject: [Histonet] Gomori Aldehyde Fuchsin and Control Blocks In-Reply-To: <1C152FCB03A4F84197818DEE847F0D4A038F3D16@stampy.sktnhr.ca> References: <1C152FCB03A4F84197818DEE847F0D4A038F3D16@stampy.sktnhr.ca> Message-ID: <47AC8303.60309@umdnj.edu> Hi Sharron: You can use acetaldehyde instead of paraldehyde but you must use three times as much (one paraldehyde dissociates to 3 acetaldehydes in the stain). See "Staining properties of aldehyde fuchsin analogs" by Buehner et al. J. Histochem. Cytochem 27(3);782-787, 1979. If you can get your pathologist to write an Rx for parladehyde get in the little ampoules so you always have fresh. Using acetaldehyde is easier! And make sure your "basic fucsin" is really pararosanaline. Geoff Genest, Sharon SktnHR wrote: > I am having difficulty obtaining paraldehyde for this method does anyone > have suggestions for and alternate method that does not use this? I > already have Movat and Verhoeff but some Pathologists prefer the GAF. > > Does anyone have a supplier with a Canadian distributor that I can > purchase control blocks for amoebae, spirochetes, micobacterium leprae? > > Sharon Genest MLT > Acting Technologist III > Histology Saskatoon Health Region > Phone: (306)655-8197 > Email: sharon.genest@saskatoonhealthregion.ca > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From rjbuesa <@t> yahoo.com Fri Feb 8 10:35:07 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 8 10:35:12 2008 Subject: [Histonet] Histology Grade Solvents In-Reply-To: <47AC0938.11C6.00D6.0@mrl.ubc.ca> Message-ID: <644846.92245.qm@web61212.mail.yahoo.com> Some manufacturers qualify a solvent that is NOT chemically pure (not good for chemical quantitative analytical purposes) BUT "pure enough" to be used in histology, specially for processing or staining. I have always taken exception to this "histology grade" issue because it is somewhat demeaning, as if what we do do not deserve a good quality reagent grade chemical. Those are the standard to prepare our stock staining solutions that should be as pure as possible, especially when silver staining is involved. So, "histology grade" reagent is one that we can use, but that a chemist "should not". Ren? J. Mark Elliott wrote: Good Friday Morning to everyone.-YEAHHH!! It's been one of those weeks:( I was asked by someone about this and not sure what the answer is. "I have a quick question for you regarding histology grade solvents. Many companies use this as a grade but is there an official specification for this grade? If so, could you direct me to the document." Any ideas?? Thanks Mark ***CONFIDENTIALITY NOTICE*** This electronic message is intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From asmith <@t> mail.barry.edu Fri Feb 8 10:56:48 2008 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Feb 8 10:57:01 2008 Subject: [Histonet] Histology Grade Solvents In-Reply-To: <644846.92245.qm@web61212.mail.yahoo.com> Message-ID: The "Histology grade" label on solvents is not at all demeaning; it just notes that that bottle of solvent is fit for use in histology. Histology grade xylene is a mixture of 3 different xylenes and ethylbenzene: it clears tissue beautifully, but the extra absorption peaks would be a real pain in a solvent for spectroscopy, GC, or HPLC. Ethanol denatured with kerosene is a good solvent for organic reactions because kerosene is not very reactive, but its lack of miscibility with water is disastrous in a histology lab. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, February 08, 2008 11:35 AM To: Mark Elliott; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Histology Grade Solvents Some manufacturers qualify a solvent that is NOT chemically pure (not good for chemical quantitative analytical purposes) BUT "pure enough" to be used in histology, specially for processing or staining. I have always taken exception to this "histology grade" issue because it is somewhat demeaning, as if what we do do not deserve a good quality reagent grade chemical. Those are the standard to prepare our stock staining solutions that should be as pure as possible, especially when silver staining is involved. So, "histology grade" reagent is one that we can use, but that a chemist "should not". Ren? J. Mark Elliott wrote: Good Friday Morning to everyone.-YEAHHH!! It's been one of those weeks:( I was asked by someone about this and not sure what the answer is. "I have a quick question for you regarding histology grade solvents. Many companies use this as a grade but is there an official specification for this grade? If so, could you direct me to the document." Any ideas?? Thanks Mark ***CONFIDENTIALITY NOTICE*** This electronic message is intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From melissa.mazan <@t> tufts.edu Fri Feb 8 12:00:06 2008 From: melissa.mazan <@t> tufts.edu (Melissa Mazan) Date: Fri Feb 8 12:00:11 2008 Subject: [Histonet] macrophage staining In-Reply-To: <200802081636.m18Ga20k014818@mail-proofpoint-2a.usg.tufts.edu> References: <200802081636.m18Ga20k014818@mail-proofpoint-2a.usg.tufts.edu> Message-ID: <47AC98A6.30606@tufts.edu> Hi all, I'm trying to do fluorescent immunostaining of macrophages on FFPE mouse tissues - I have tried the BD monoclonal Mac-3 (raised in rat) - it works well with the Vector ABC system, but cannot get it to work well with fluorescence. Tried a simple primary, 594 conjugated secondary approach - no signal. Then tried a biotinylated secondary followed with streptavidin - get a messy, non-uniform signal. Does anyone have an antibody and protocol for fluorescent labeling of macrophages on formalin fixed paraffin embedded tissue? Many thanks - Melissa -- Melissa R. Mazan, DVM, Diplomate ACVIM Associate Professor and Director of Sports Medicine Tufts Cummings School of Veterinary Medicine 200 Westboro Road North Grafton, MA 01536 508-887-4589 From RSRICHMOND <@t> aol.com Fri Feb 8 12:09:59 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Fri Feb 8 12:10:04 2008 Subject: [Histonet] Re: Histology Grade Solvents Message-ID: In chemistry, it's a good practice never to buy more purity than you need. Look at common chemicals in the Sigma catalog - there can be dozens of grades of a chemical for different purposes. But Mark Elliott's original question hasn't been answered - "I have a quick question for you regarding histology grade solvents. Many companies use this as a grade but is there an official specification for this grade? If so, could you direct me to the document." Bob Richmond Samurai Pathologist Knoxville TN From cormier <@t> MIT.EDU Fri Feb 8 12:23:07 2008 From: cormier <@t> MIT.EDU (Kathy Cormier) Date: Fri Feb 8 12:23:14 2008 Subject: [Histonet] macrophage staining References: <200802081636.m18Ga20k014818@mail-proofpoint-2a.usg.tufts.edu> <47AC98A6.30606@tufts.edu> Message-ID: <001001c86a7f$a74113f0$92003712@mit.edu> Hi Melissa, We use Caltag # mf48105 rat ab on mouse FFPE tissue w/ great sucess. (primary at 1:100, secondary at 1:100, strepav fitc at 1:100) (HIER ph6 first though) Kathy Cormier Div Comp Med MIT ----- Original Message ----- From: "Melissa Mazan" To: Sent: Friday, February 08, 2008 1:00 PM Subject: [Histonet] macrophage staining > Hi all, > I'm trying to do fluorescent immunostaining of macrophages on FFPE mouse > tissues - I have tried the BD monoclonal Mac-3 (raised in rat) - it works > well with the Vector ABC system, but cannot get it to work well with > fluorescence. Tried a simple primary, 594 conjugated secondary approach - > no signal. Then tried a biotinylated secondary followed with > streptavidin - get a messy, non-uniform signal. Does anyone have an > antibody and protocol for fluorescent labeling of macrophages on formalin > fixed paraffin embedded tissue? Many thanks - Melissa > > > -- > Melissa R. Mazan, DVM, Diplomate ACVIM > Associate Professor and Director of Sports Medicine > Tufts Cummings School of Veterinary Medicine > 200 Westboro Road > North Grafton, MA 01536 > 508-887-4589 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sqd3f <@t> cms.mail.virginia.edu Fri Feb 8 13:24:20 2008 From: sqd3f <@t> cms.mail.virginia.edu (Sonny Duong) Date: Fri Feb 8 13:24:25 2008 Subject: [Histonet] Fecal Fat Message-ID: <108BFDFEE6837B92C4BEFF96@[192.168.2.102]> Hi all, Does anyone have a good protocol for fecal fat microscopy with Oil Red O or Sudan III? Thanks! Sonny From carl.hobbs <@t> kcl.ac.uk Fri Feb 8 13:26:50 2008 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Fri Feb 8 13:27:31 2008 Subject: [Histonet] Re: Histology Grade Solvents Message-ID: <001101c86a88$8dc39200$4001a8c0@carlba65530bda> I agree with Allen. Xylene is a good example, that I understand: "histological grade" is low in sulphur. Many years ago, I found that when I used xylene that was not low in sulphur, my H&Es after a week or so, showed great nuclear staining but...the eosin had "disappeared"! ( I cleared sections in this xylene and mounted in DPX that had been thinned in same xylene) "Fit for the purpose"......I always liked that phrase ;-) I love being a Histologist but, in context, my only regret is that I am not also a Bio/Chemist! Horobin, Pearce, Lake, Kiernan...where are your Successors? Rene, it's Friday I noticed ;-) Carl PS: Rene, many thanks for your tips re my wax/tissue separation problem. Equal thanks to Gayle. Sure, not solved but, that's my job to go figure it out, using helpful hints and tips from Histonet! Thanks. From bernardgerard <@t> yahoo.com Fri Feb 8 13:43:27 2008 From: bernardgerard <@t> yahoo.com (Bernard Martin) Date: Fri Feb 8 13:43:30 2008 Subject: [Histonet] trichrome and immunohistochemistry Message-ID: <502225.58962.qm@web37901.mail.mud.yahoo.com> Has anyone ever counterstained IHC with trichrome, or heard of it being done? Is this even possible? Thanks! ____________________________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ From bernardgerard <@t> yahoo.com Fri Feb 8 13:45:44 2008 From: bernardgerard <@t> yahoo.com (Bernard Martin) Date: Fri Feb 8 13:45:47 2008 Subject: [Histonet] IHC unclear at 40x Message-ID: <694666.71542.qm@web37902.mail.mud.yahoo.com> My IHC looks great at 5x, 10x, and 20x, but when I go up to 40x, it is a bit blurry. The objective is clean, as is the slide itself. I deparaffinate in clean xylene. Anyone run into this problem, and if so, how did you resolve it? Thanks! ____________________________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ From liz <@t> premierlab.com Fri Feb 8 13:50:01 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Feb 8 13:50:14 2008 Subject: [Histonet] trichrome and immunohistochemistry In-Reply-To: <1038731D80184E949B38323D11C5F9BC@PremierLab.local> References: <1038731D80184E949B38323D11C5F9BC@PremierLab.local> Message-ID: As long as you use DAB as the chromogen you should be fine, have not done the trichrome, but have ran other special stains on top of IHC such as iron, AFB, etc. I'm not sure how it will look, you might need to modify the trichrome a bit. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernard Martin Sent: Friday, February 08, 2008 12:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] trichrome and immunohistochemistry Has anyone ever counterstained IHC with trichrome, or heard of it being done? Is this even possible? Thanks! ________________________________________________________________________ ____________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Fri Feb 8 14:02:23 2008 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Feb 8 14:02:28 2008 Subject: [Histonet] microtome repair Message-ID: We have a Shandon Finesse 315 microtome in need of repairs. We are located in Southern California. It is difficult to find anyone to repair these microtomes, other than Thermo. My hesitancy with Thermo is the guesstimate that I was given. $200 per hour travel time, mileage, labor and parts. The estimate is for $1500 to $2000. This is way beyond the budget of a community college histo program, particularly since this is the second microtome to have a similar problem. The microtomes are a little over two years old with minimal usage. Doe anyone know of a reasonable company that can repair these microtomes? Thank you, Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu From anh2006 <@t> med.cornell.edu Fri Feb 8 14:12:13 2008 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Fri Feb 8 14:12:36 2008 Subject: [Histonet] IHC unclear at 40x In-Reply-To: <694666.71542.qm@web37902.mail.mud.yahoo.com> References: <694666.71542.qm@web37902.mail.mud.yahoo.com> Message-ID: 99/100 that's from a blurry objective. 40X commonly are swiped through oil or worse yet, through wet mounting media (heavens forbid). So I suggest that you reclean your objective, even though you think it's clean. At 11:45 AM -0800 2/8/08, Bernard Martin wrote: >My IHC looks great at 5x, 10x, and 20x, but when I go >up to 40x, it is a bit blurry. The objective is >clean, as is the slide itself. I deparaffinate in >clean xylene. Anyone run into this problem, and if >so, how did you resolve it? Thanks! > > -- From katherine-walters <@t> uiowa.edu Fri Feb 8 14:31:07 2008 From: katherine-walters <@t> uiowa.edu (Walters, Katherine S) Date: Fri Feb 8 14:31:13 2008 Subject: [Histonet] trichrome and immunohistochemistry In-Reply-To: Message-ID: I have done this with DAB and Masson's trichrome. The DAB turns purplish, but once you get used to the color change, it works pretty well. I left out the hematoxylin, so I guess it's a bichrome. Kathy Walters Katherine Walters Histology Director Central Microscopy Research Facility University of Iowa 85 Eckstein Medical Research Building Iowa City, Iowa 52242-1101 phone: (319) 335-8142 fax: (319) 384-4469 katherine-walters@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Friday, February 08, 2008 1:50 PM To: Bernard Martin; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] trichrome and immunohistochemistry As long as you use DAB as the chromogen you should be fine, have not done the trichrome, but have ran other special stains on top of IHC such as iron, AFB, etc. I'm not sure how it will look, you might need to modify the trichrome a bit. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernard Martin Sent: Friday, February 08, 2008 12:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] trichrome and immunohistochemistry Has anyone ever counterstained IHC with trichrome, or heard of it being done? Is this even possible? Thanks! ________________________________________________________________________ ____________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Feb 8 14:46:19 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 8 14:46:24 2008 Subject: [Histonet] IHC unclear at 40x In-Reply-To: <694666.71542.qm@web37902.mail.mud.yahoo.com> Message-ID: <17970.91494.qm@web61225.mail.yahoo.com> Make sure that when you finish and dehydrate to coverslip, that dehydration is complete before clearing the section with xylene. Ren? J. Bernard Martin wrote: My IHC looks great at 5x, 10x, and 20x, but when I go up to 40x, it is a bit blurry. The objective is clean, as is the slide itself. I deparaffinate in clean xylene. Anyone run into this problem, and if so, how did you resolve it? Thanks! ____________________________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From pmarcum <@t> vet.upenn.edu Fri Feb 8 14:52:34 2008 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Fri Feb 8 14:52:39 2008 Subject: [Histonet] Mouse pups and processing Message-ID: <6.2.5.6.2.20080208154217.01c8a9b8@vet.upenn.edu> I have been struggling with this for a while and need some help. We currently have a project with 0 day mouse pups that are allowed to be born normally and then sacrificed with CO2. We had several groups earlier that were sacrificed a different way and they processed and sectioned beautifully. These don't seem to fix well, dehydrate, clear or infiltrate worth a darn. Since this is my first time using CO2 for sacrifice I need to find out if it causes a problem or if I am just losing it. I have not ever had this problem before and even re-processing does not help. I know they are not infiltrating as they are floating in paraffin at the end if I remove them from the processor to a vat of paraffin. They will not sink. The pups are 1.2 to 1.3 grams each. If you can suggest a better way to sacrifice them please let me know. Killing the mother and perfusing her is not an option as these are not our mice. They are being given as favor so I am limited to some extent. This was an overnight process with slightly altered alcohols to 45 minutes each, 1 xylene at 30 minutes and 2 Shandon Xylene Substitues at 10 hour each to 4 paraffins at 45 X2 and 1 hour X2. Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From derek.papalegis <@t> tufts.edu Fri Feb 8 15:06:40 2008 From: derek.papalegis <@t> tufts.edu (Derek Papalegis) Date: Fri Feb 8 15:06:44 2008 Subject: [Histonet] Mouse pups and processing In-Reply-To: <6.2.5.6.2.20080208154217.01c8a9b8@vet.upenn.edu> References: <6.2.5.6.2.20080208154217.01c8a9b8@vet.upenn.edu> Message-ID: <47ACC460.3070408@tufts.edu> Pamela Marcum wrote: > > > I have been struggling with this for a while and need some help. We > currently have a project with 0 day mouse pups that are allowed to be > born normally and then sacrificed with CO2. We had several groups > earlier that were sacrificed a different way and they processed and > sectioned beautifully. > > These don't seem to fix well, dehydrate, clear or infiltrate worth a > darn. Since this is my first time using CO2 for sacrifice I need to > find out if it causes a problem or if I am just losing it. I have not > ever had this problem before and even re-processing does not help. I > know they are not infiltrating as they are floating in paraffin at the > end if I remove them from the processor to a vat of paraffin. They > will not sink. The pups are 1.2 to 1.3 grams each. > > If you can suggest a better way to sacrifice them please let me know. > Killing the mother and perfusing her is not an option as these are not > our mice. They are being given as favor so I am limited to some extent. > > This was an overnight process with slightly altered alcohols to 45 > minutes each, 1 xylene at 30 minutes and 2 Shandon Xylene Substitues > at 10 hour each to 4 paraffins at 45 X2 and 1 hour X2. > > Best Regards, > > Pamela A Marcum > Manager, Histology Special Procedures > University of Pennsylvania > School of Veterinary Medicine > R.S. Reynolds Jr. CORL > New Bolton Center > 382 West Street Road > Kennett Square, PA 19348 > > Phone - 610-925-6278 > Fax - 610-925-8120 > E-mail - pmarcum@vet.upenn.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet Hi Pamela, I would recommend using decapitation as your euthanasia method instead of CO2. Use Bouin's to fix instead of formalin. I usually decapitate and then slightly cut the abdomen with a razor blade to help the fixative penetrate. I have left pups in Bouin's up to 48 hours with no adverse affects.How you proceed really depends on what sections you want. Most investigators I have encountered initially want longitudinal sections cut but they soon realize that this does not yield any useful information from the slide. Longitudinal sections "look pretty" on the slide but they are practically useless to demonstrate all the organs. The best way to make sections of pups is to take 5-7 cross sections after it is completely fixed. I have a diagram of a pup with lines through it showing me where to take my sections from. This ensures consistency from pup to pup. I just process the pups on my normal processing schedule and have had great results from this. Feel free to email me if you have any questions about this or would like me to send you the pup diagram of where to take sections from. Good luck Derek -- Derek Papalegis HT (ASCP) Histotechnician Division of Laboratory Animal Medicine Tufts University 136 Harrison Avenue Boston, MA 02111 phone: 617 636-2971 fax: 617 636-8354 From anh2006 <@t> med.cornell.edu Fri Feb 8 15:08:35 2008 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Fri Feb 8 15:08:47 2008 Subject: [Histonet] IHC unclear at 40x In-Reply-To: <17970.91494.qm@web61225.mail.yahoo.com> References: <17970.91494.qm@web61225.mail.yahoo.com> Message-ID: One more thought, are your slides fully dry? I find difficulties in focussing sometimes with slides which are still wet. It's better to allow time to dry, safer for your scope as well. > > >Bernard Martin wrote: > My IHC looks great at 5x, 10x, and 20x, but when I go >up to 40x, it is a bit blurry. The objective is >clean, as is the slide itself. I deparaffinate in >clean xylene. Anyone run into this problem, and if >so, how did you resolve it? Thanks! > -- From jfish <@t> gladstone.ucsf.edu Fri Feb 8 15:21:52 2008 From: jfish <@t> gladstone.ucsf.edu (Jo Dee Fish) Date: Fri Feb 8 15:22:00 2008 Subject: [Histonet] IHC unclear at 40x In-Reply-To: <17970.91494.qm@web61225.mail.yahoo.com> References: <694666.71542.qm@web37902.mail.mud.yahoo.com> <17970.91494.qm@web61225.mail.yahoo.com> Message-ID: <000b01c86a98$9f908140$2e0d010a@JFISH> Bernard, Don't forget to do Koehler illumination procedure to your microscope as well. If you need instructions, try here: http://www.rawlight.com/Koehler_illumination.pdf Take care, Jo Dee ~~Jo Dee Fish~~ Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, February 08, 2008 12:46 PM To: Bernard Martin; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC unclear at 40x Make sure that when you finish and dehydrate to coverslip, that dehydration is complete before clearing the section with xylene. Ren? J. Bernard Martin wrote: My IHC looks great at 5x, 10x, and 20x, but when I go up to 40x, it is a bit blurry. The objective is clean, as is the slide itself. I deparaffinate in clean xylene. Anyone run into this problem, and if so, how did you resolve it? Thanks! ____________________________________________________________________________ ________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Fri Feb 8 15:39:40 2008 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Feb 8 15:39:39 2008 Subject: [Histonet] microtome repair In-Reply-To: Message-ID: Oops, I meant a Shandon Finesse 325. Jennifer MacDonald Sent by: histonet-bounces@lists.utsouthwestern.edu 02/08/2008 12:02 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] microtome repair We have a Shandon Finesse 315 microtome in need of repairs. We are located in Southern California. It is difficult to find anyone to repair these microtomes, other than Thermo. My hesitancy with Thermo is the guesstimate that I was given. $200 per hour travel time, mileage, labor and parts. The estimate is for $1500 to $2000. This is way beyond the budget of a community college histo program, particularly since this is the second microtome to have a similar problem. The microtomes are a little over two years old with minimal usage. Doe anyone know of a reasonable company that can repair these microtomes? Thank you, Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asachau <@t> titanmed.com Fri Feb 8 16:21:40 2008 From: asachau <@t> titanmed.com (April Sachau) Date: Fri Feb 8 16:24:33 2008 Subject: [Histonet] Great Histology Position Available!!! In-Reply-To: Message-ID: <7E3ACD48BA6E26408F3188FBF08693F7010D4806@titansbs1.corp.titanmed.com> Hello Histonetters! I have a position available in the wonderful Midwest! This is a temporary (3 month) assignment and it has great compensation package! ASCP certification is required! Please contact me if you are interested or would like more details! April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com From koellingr <@t> comcast.net Fri Feb 8 17:13:41 2008 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Fri Feb 8 17:13:45 2008 Subject: [Histonet] Mouse pups and processing Message-ID: <020820082313.19671.47ACE224000CC19A00004CD722070206539D09020704040A0105@comcast.net> Pam, I think Dereks information regarding decapitation is very good and I don't have much to say about fixation and processing in addition to his but I would say sit with your IACUC committee immediately before you do any more euthanasia or accept anything from someone who hasn't gone through IACUC. Euthanasia procedures for such pups are completely different than for adults because of their resistance to hypoxia and the CO2 euthanasia. Every committee follows (or should follow) rigid standards for the welfare of animals being sacraficed and can differ in things such as length of time, cycles in CO2, paper in the chamber, secondary euthanasia techniques, who can do this and who can't. I've seen pups come out of CO2 chambers for extended times and still be alive and start kicking due to their built in CO2 resistence. I think it is our moral duty to see that animals suffer as absolutely little as is possible and that is why IACUC committee's exist and their regulations rigid. Ray -------------- Original message -------------- From: Derek Papalegis > Pamela Marcum wrote: > > > > > > I have been struggling with this for a while and need some help. We > > currently have a project with 0 day mouse pups that are allowed to be > > born normally and then sacrificed with CO2. We had several groups > > earlier that were sacrificed a different way and they processed and > > sectioned beautifully. > > > > These don't seem to fix well, dehydrate, clear or infiltrate worth a > > darn. Since this is my first time using CO2 for sacrifice I need to > > find out if it causes a problem or if I am just losing it. I have not > > ever had this problem before and even re-processing does not help. I > > know they are not infiltrating as they are floating in paraffin at the > > end if I remove them from the processor to a vat of paraffin. They > > will not sink. The pups are 1.2 to 1.3 grams each. > > > > If you can suggest a better way to sacrifice them please let me know. > > Killing the mother and perfusing her is not an option as these are not > > our mice. They are being given as favor so I am limited to some extent. > > > > This was an overnight process with slightly altered alcohols to 45 > > minutes each, 1 xylene at 30 minutes and 2 Shandon Xylene Substitues > > at 10 hour each to 4 paraffins at 45 X2 and 1 hour X2. > > > > Best Regards, > > > > Pamela A Marcum > > Manager, Histology Special Procedures > > University of Pennsylvania > > School of Veterinary Medicine > > R.S. Reynolds Jr. CORL > > New Bolton Center > > 382 West Street Road > > Kennett Square, PA 19348 > > > > Phone - 610-925-6278 > > Fax - 610-925-8120 > > E-mail - pmarcum@vet.upenn.edu > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Hi Pamela, > I would recommend using decapitation as your euthanasia method instead > of CO2. Use Bouin's to fix instead of formalin. I usually decapitate and > then slightly cut the abdomen with a razor blade to help the fixative > penetrate. I have left pups in Bouin's up to 48 hours with no adverse > affects.How you proceed really depends on what sections you want. Most > investigators I have encountered initially want longitudinal sections > cut but they soon realize that this does not yield any useful > information from the slide. Longitudinal sections "look pretty" on the > slide but they are practically useless to demonstrate all the organs. > The best way to make sections of pups is to take 5-7 cross sections > after it is completely fixed. I have a diagram of a pup with lines > through it showing me where to take my sections from. This ensures > consistency from pup to pup. I just process the pups on my normal > processing schedule and have had great results from this. > > Feel free to email me if you have any questions about this or would like > me to send you the pup diagram of where to take sections from. > > Good luck > > Derek > > -- > Derek Papalegis HT (ASCP) > Histotechnician > Division of Laboratory Animal Medicine > Tufts University > 136 Harrison Avenue > Boston, MA 02111 > phone: 617 636-2971 > fax: 617 636-8354 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr <@t> comcast.net Fri Feb 8 17:20:25 2008 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Fri Feb 8 17:20:31 2008 Subject: [Histonet] IHC unclear at 40x Message-ID: <020820082320.29004.47ACE3B90002BAA00000714C22070206539D09020704040A0105@comcast.net> Bernard, All the suggestions I saw, oil, dehydration, dry, Koehler illumination are great. Is another possibility that you have a correction collar (like for coverslip thickness) on the barrel of your 40x? Some don't but many do. It is easy to be clear at 40x and purposely move the correction collar to become blurry. If you don't know its there, it just might be where it has been placed and has been all along and needs to be adjusted by simply slightly revolving into focus Ray Koelling PhenoPath Labs Seattle, WA -------------- Original message -------------- From: "Jo Dee Fish" > Bernard, > Don't forget to do Koehler illumination procedure to your microscope as > well. If you need instructions, try here: > http://www.rawlight.com/Koehler_illumination.pdf > Take care, > Jo Dee > > > ~~Jo Dee Fish~~ > Research Technologist III > Gladstone Institute of Cardiovascular Disease > > Telephone: (415) 734-2567 > Fax: (415) 355-0824 > E-mail: jfish@gladstone.ucsf.edu > > Mailing address: > The J. David Gladstone Institutes > 1650 Owens Street > San Francisco, CA 94158 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Friday, February 08, 2008 12:46 PM > To: Bernard Martin; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] IHC unclear at 40x > > Make sure that when you finish and dehydrate to coverslip, that dehydration > is complete before clearing the section with xylene. > RenêÂJ. > > Bernard Martin wrote: > My IHC looks great at 5x, 10x, and 20x, but when I go up to 40x, it is a > bit blurry. The objective is clean, as is the slide itself. I deparaffinate > in clean xylene. Anyone run into this problem, and if so, how did you > resolve it? Thanks! > > > ____________________________________________________________________________ > ________ > Be a better friend, newshound, and > know-it-all with Yahoo! Mobile. Try it now. > http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Looking for last minute shopping deals? Find them fast with Yahoo! Search. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Fri Feb 8 17:34:51 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Feb 8 17:34:57 2008 Subject: [Histonet] OT: the weather References: <000101c86a5e$43c08660$d00f7ca5@lurie.northwestern.edu> Message-ID: <004901c86aab$342a5300$0302a8c0@yourxhtr8hvc4p> It's Friday and I haven't been flamed in a while. Weather records have been around for what? 100 years or so? How old is our planet, millions of years? So you think that 100 years of data can be spread across millions of years? Here's a new thought. What if this is just a natural occasion between ice ages? JTT ----- Original Message ----- From: "Bernice Frederick" To: "'Joe Nocito'" ; "'Molinari, Betsy'" ; Sent: Friday, February 08, 2008 8:24 AM Subject: RE: [Histonet] OT: the weather You realize we'll be told that we got all this snow because of global warming? Just because we haven't had a real Chicago winter in a while people are freaking out. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, February 07, 2008 8:04 PM To: Molinari, Betsy; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] OT: the weather so much for global warning ----- Original Message ----- From: "Molinari, Betsy" To: Sent: Thursday, February 07, 2008 5:45 AM Subject: RE: [Histonet] OT: the weather Wow! Looks more like Colorado or Wyoming! Betsy Molinari HT(ASCP) Cardiovascular Pathology Texas Heart Institute 6770 Bertner Ave. MC 1-283 Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Van Patten Sent: Wednesday, February 06, 2008 11:02 AM To: Jackie M O'Connor; kemlo Cc: histonet@lists.utsouthwestern.edu; Ingles Claire Subject: RE: [Histonet] OT: the weather Strange weather across the country! Even Maui and the "Big Island" got snow 1 week ago... http://starbulletin.com/2008/01/30/news/story01.html -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Wednesday, February 06, 2008 10:38 AM To: kemlo Cc: histonet@lists.utsouthwestern.edu; 'Ingles Claire' Subject: RE: [Histonet] OT: the weather Up here in Illinois near the Wisconsin border - we're expecting 12-14 inches of snow, and most of the staff have been allowed to go home. I just read that Hawaii has had the worst rainfall in decades, 12" of rain in 24 hours in Hilo - still not enough to extinguish the volcano, tho. (Yes, I am kidding about the volcano). "kemlo" Sent by: histonet-bounces@lists.utsouthwestern.edu 02/06/2008 10:26 AM To "'Ingles Claire'" cc histonet@lists.utsouthwestern.edu Subject RE: [Histonet] OT: the weather We across the pond are constructing an Ark; there's enough rain to float it and enough wind to give it the kinetic energy it requires. I blame...... The no smoking policy. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: 06 February 2008 15:06 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OT: the weather Hey guys: I don't know about you, but I'm really beginning to wonder about the weather. Anyway- everyone in the southern states, especially Arkansas, still there? I gripe about the snow this year, but geez. How bad was the damage from the tornadoes down in that area. Just thinking of everyone, and hoping all is well with everyone. Stay warm/dry/unburnt/etc. You bunch from across the pond can send us your extra show shovels and inflatable rafts. Anywhere you could put us up if it gets too bad over here? :) Claire _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sat Feb 9 09:36:08 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Feb 9 09:36:12 2008 Subject: [Histonet] trichrome and immunohistochemistry In-Reply-To: <502225.58962.qm@web37901.mail.mud.yahoo.com> Message-ID: <505856.50192.qm@web61211.mail.yahoo.com> It can be done but it also depends on the epitope locaiotn. If it is nuclear you cannot use the iron hematoxylin, and if it is cytoplasmic you have to go with a very light cytoplasmic staining step. The only thing I wonder is why would you want to do that? IHC "standard" is running the procedure itself up to the DAB and ending with a very light hematoxylin (any regressive for 30 seconds with just blueing). Ren? J. Bernard Martin wrote: Has anyone ever counterstained IHC with trichrome, or heard of it being done? Is this even possible? Thanks! ____________________________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From pruegg <@t> ihctech.net Sat Feb 9 11:02:15 2008 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Sat Feb 9 10:55:58 2008 Subject: [Histonet] OT: the weather In-Reply-To: <004901c86aab$342a5300$0302a8c0@yourxhtr8hvc4p> Message-ID: <200802091655.m19Gtkoo091582@pro12.abac.com> We don't complain about snow in the mountains in Colorado that is where our water comes from, but this year in west central CO they have the most snow they have ever seen, the place they carry the snow off too out of town is full, they have the snow piled 8 feet high between the road lanes and now they just got another 2 feet dumped on them, I am glad I live on the plains at the base of the mountains this year because we only have a dusting of snow now. Patsy in Colorado Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, February 08, 2008 4:35 PM To: Bernice Frederick; 'Molinari, Betsy'; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] OT: the weather It's Friday and I haven't been flamed in a while. Weather records have been around for what? 100 years or so? How old is our planet, millions of years? So you think that 100 years of data can be spread across millions of years? Here's a new thought. What if this is just a natural occasion between ice ages? JTT ----- Original Message ----- From: "Bernice Frederick" To: "'Joe Nocito'" ; "'Molinari, Betsy'" ; Sent: Friday, February 08, 2008 8:24 AM Subject: RE: [Histonet] OT: the weather You realize we'll be told that we got all this snow because of global warming? Just because we haven't had a real Chicago winter in a while people are freaking out. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, February 07, 2008 8:04 PM To: Molinari, Betsy; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] OT: the weather so much for global warning ----- Original Message ----- From: "Molinari, Betsy" To: Sent: Thursday, February 07, 2008 5:45 AM Subject: RE: [Histonet] OT: the weather Wow! Looks more like Colorado or Wyoming! Betsy Molinari HT(ASCP) Cardiovascular Pathology Texas Heart Institute 6770 Bertner Ave. MC 1-283 Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Van Patten Sent: Wednesday, February 06, 2008 11:02 AM To: Jackie M O'Connor; kemlo Cc: histonet@lists.utsouthwestern.edu; Ingles Claire Subject: RE: [Histonet] OT: the weather Strange weather across the country! Even Maui and the "Big Island" got snow 1 week ago... http://starbulletin.com/2008/01/30/news/story01.html -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Wednesday, February 06, 2008 10:38 AM To: kemlo Cc: histonet@lists.utsouthwestern.edu; 'Ingles Claire' Subject: RE: [Histonet] OT: the weather Up here in Illinois near the Wisconsin border - we're expecting 12-14 inches of snow, and most of the staff have been allowed to go home. I just read that Hawaii has had the worst rainfall in decades, 12" of rain in 24 hours in Hilo - still not enough to extinguish the volcano, tho. (Yes, I am kidding about the volcano). "kemlo" Sent by: histonet-bounces@lists.utsouthwestern.edu 02/06/2008 10:26 AM To "'Ingles Claire'" cc histonet@lists.utsouthwestern.edu Subject RE: [Histonet] OT: the weather We across the pond are constructing an Ark; there's enough rain to float it and enough wind to give it the kinetic energy it requires. I blame...... The no smoking policy. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: 06 February 2008 15:06 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OT: the weather Hey guys: I don't know about you, but I'm really beginning to wonder about the weather. Anyway- everyone in the southern states, especially Arkansas, still there? I gripe about the snow this year, but geez. How bad was the damage from the tornadoes down in that area. Just thinking of everyone, and hoping all is well with everyone. Stay warm/dry/unburnt/etc. You bunch from across the pond can send us your extra show shovels and inflatable rafts. Anywhere you could put us up if it gets too bad over here? :) Claire _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Feb 9 11:11:57 2008 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Sat Feb 9 11:05:51 2008 Subject: [Histonet] Mouse pups and processing In-Reply-To: <020820082313.19671.47ACE224000CC19A00004CD722070206539D09020704040A0105@comcast.net> Message-ID: <200802091705.m19H5Skv096214@pro12.abac.com> I agree decapitation is the way to go with pups, that way you can get them fixed quickly. We even dissect the heads longitudinally in the middle between the eyes down the middle of the nose because we are interested in sinus development. After fixation in NBF for 24 hrs. I process these on a regular 1 hour per reagent tissue processing schedule, they cut and look really good. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of koellingr@comcast.net Sent: Friday, February 08, 2008 4:14 PM To: Derek Papalegis; Pamela Marcum Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Mouse pups and processing Pam, I think Dereks information regarding decapitation is very good and I don't have much to say about fixation and processing in addition to his but I would say sit with your IACUC committee immediately before you do any more euthanasia or accept anything from someone who hasn't gone through IACUC. Euthanasia procedures for such pups are completely different than for adults because of their resistance to hypoxia and the CO2 euthanasia. Every committee follows (or should follow) rigid standards for the welfare of animals being sacraficed and can differ in things such as length of time, cycles in CO2, paper in the chamber, secondary euthanasia techniques, who can do this and who can't. I've seen pups come out of CO2 chambers for extended times and still be alive and start kicking due to their built in CO2 resistence. I think it is our moral duty to see that animals suffer as absolutely little as is possible and that is why IACUC committee's exist and their regulations rigid. Ray -------------- Original message -------------- From: Derek Papalegis > Pamela Marcum wrote: > > > > > > I have been struggling with this for a while and need some help. We > > currently have a project with 0 day mouse pups that are allowed to be > > born normally and then sacrificed with CO2. We had several groups > > earlier that were sacrificed a different way and they processed and > > sectioned beautifully. > > > > These don't seem to fix well, dehydrate, clear or infiltrate worth a > > darn. Since this is my first time using CO2 for sacrifice I need to > > find out if it causes a problem or if I am just losing it. I have not > > ever had this problem before and even re-processing does not help. I > > know they are not infiltrating as they are floating in paraffin at the > > end if I remove them from the processor to a vat of paraffin. They > > will not sink. The pups are 1.2 to 1.3 grams each. > > > > If you can suggest a better way to sacrifice them please let me know. > > Killing the mother and perfusing her is not an option as these are not > > our mice. They are being given as favor so I am limited to some extent. > > > > This was an overnight process with slightly altered alcohols to 45 > > minutes each, 1 xylene at 30 minutes and 2 Shandon Xylene Substitues > > at 10 hour each to 4 paraffins at 45 X2 and 1 hour X2. > > > > Best Regards, > > > > Pamela A Marcum > > Manager, Histology Special Procedures > > University of Pennsylvania > > School of Veterinary Medicine > > R.S. Reynolds Jr. CORL > > New Bolton Center > > 382 West Street Road > > Kennett Square, PA 19348 > > > > Phone - 610-925-6278 > > Fax - 610-925-8120 > > E-mail - pmarcum@vet.upenn.edu > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Hi Pamela, > I would recommend using decapitation as your euthanasia method instead > of CO2. Use Bouin's to fix instead of formalin. I usually decapitate and > then slightly cut the abdomen with a razor blade to help the fixative > penetrate. I have left pups in Bouin's up to 48 hours with no adverse > affects.How you proceed really depends on what sections you want. Most > investigators I have encountered initially want longitudinal sections > cut but they soon realize that this does not yield any useful > information from the slide. Longitudinal sections "look pretty" on the > slide but they are practically useless to demonstrate all the organs. > The best way to make sections of pups is to take 5-7 cross sections > after it is completely fixed. I have a diagram of a pup with lines > through it showing me where to take my sections from. This ensures > consistency from pup to pup. I just process the pups on my normal > processing schedule and have had great results from this. > > Feel free to email me if you have any questions about this or would like > me to send you the pup diagram of where to take sections from. > > Good luck > > Derek > > -- > Derek Papalegis HT (ASCP) > Histotechnician > Division of Laboratory Animal Medicine > Tufts University > 136 Harrison Avenue > Boston, MA 02111 > phone: 617 636-2971 > fax: 617 636-8354 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jflinn <@t> gmu.edu Sat Feb 9 11:56:15 2008 From: jflinn <@t> gmu.edu (Jane M Flinn) Date: Sat Feb 9 11:56:27 2008 Subject: [Histonet] Mouse pups and processing In-Reply-To: <200802091705.m19H5Skv096214@pro12.abac.com> References: <020820082313.19671.47ACE224000CC19A00004CD722070206539D09020704040A0105@comcast.net> <200802091705.m19H5Skv096214@pro12.abac.com> Message-ID: I would agree that one needs to check with the IUCUC group. They were unhappy with our using CO2 and recomended ether. We now etherise them and then decapitate them. jane "Life is short - make haste to be kind" Dr. Jane Flinn Director, Biopsychology Program George Mason University, 3F5 4400 University Dr. Fairfax, VA 22030 Phone: 703-993-4107 Fax: 703-993-1359 ----- Original Message ----- From: patsy ruegg Date: Saturday, February 9, 2008 12:11 pm Subject: RE: [Histonet] Mouse pups and processing > I agree decapitation is the way to go with pups, that way you can > get them > fixed quickly. We even dissect the heads longitudinally in the middle > between the eyes down the middle of the nose because we are > interested in > sinus development. After fixation in NBF for 24 hrs. I process > these on a > regular 1 hour per reagent tissue processing schedule, they cut > and look > really good. > Patsy > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. #216 > Aurora, CO 80010 > 720-859-4060 > fax 720-859-4110 > pruegg@ihctech.net > www.ihctech.net > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > koellingr@comcast.net > Sent: Friday, February 08, 2008 4:14 PM > To: Derek Papalegis; Pamela Marcum > Cc: Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Mouse pups and processing > > Pam, > I think Dereks information regarding decapitation is very good and > I don't > have much to say about fixation and processing in addition to his > but I > would say sit with your IACUC committee immediately before you do > any more > euthanasia or accept anything from someone who hasn't gone through > IACUC.Euthanasia procedures for such pups are completely different > than for adults > because of their resistance to hypoxia and the CO2 euthanasia. Every > committee follows (or should follow) rigid standards for the > welfare of > animals being sacraficed and can differ in things such as length > of time, > cycles in CO2, paper in the chamber, secondary euthanasia > techniques, who > can do this and who can't. I've seen pups come out of CO2 > chambers for > extended times and still be alive and start kicking due to their > built in > CO2 resistence. I think it is our moral duty to see that animals > suffer as > absolutely little as is possible and that is why IACUC committee's > exist and > their regulations rigid. > Ray > > -------------- Original message -------------- > From: Derek Papalegis > > > Pamela Marcum wrote: > > > > > > > > > I have been struggling with this for a while and need some > help. We > > > currently have a project with 0 day mouse pups that are > allowed to be > > > born normally and then sacrificed with CO2. We had several > groups > > > earlier that were sacrificed a different way and they > processed and > > > sectioned beautifully. > > > > > > These don't seem to fix well, dehydrate, clear or infiltrate > worth a > > > darn. Since this is my first time using CO2 for sacrifice I > need to > > > find out if it causes a problem or if I am just losing it. I > have not > > > ever had this problem before and even re-processing does not > help. I > > > know they are not infiltrating as they are floating in > paraffin at the > > > end if I remove them from the processor to a vat of paraffin. > They > > > will not sink. The pups are 1.2 to 1.3 grams each. > > > > > > If you can suggest a better way to sacrifice them please let > me know. > > > Killing the mother and perfusing her is not an option as these > are not > > > our mice. They are being given as favor so I am limited to > some extent. > > > > > > This was an overnight process with slightly altered alcohols > to 45 > > > minutes each, 1 xylene at 30 minutes and 2 Shandon Xylene > Substitues > > > at 10 hour each to 4 paraffins at 45 X2 and 1 hour X2. > > > > > > Best Regards, > > > > > > Pamela A Marcum > > > Manager, Histology Special Procedures > > > University of Pennsylvania > > > School of Veterinary Medicine > > > R.S. Reynolds Jr. CORL > > > New Bolton Center > > > 382 West Street Road > > > Kennett Square, PA 19348 > > > > > > Phone - 610-925-6278 > > > Fax - 610-925-8120 > > > E-mail - pmarcum@vet.upenn.edu > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Hi Pamela, > > I would recommend using decapitation as your euthanasia method > instead > > of CO2. Use Bouin's to fix instead of formalin. I usually > decapitate and > > then slightly cut the abdomen with a razor blade to help the > fixative > > penetrate. I have left pups in Bouin's up to 48 hours with no > adverse > > affects.How you proceed really depends on what sections you > want. Most > > investigators I have encountered initially want longitudinal > sections > > cut but they soon realize that this does not yield any useful > > information from the slide. Longitudinal sections "look pretty" > on the > > slide but they are practically useless to demonstrate all the > organs. > > The best way to make sections of pups is to take 5-7 cross > sections > > after it is completely fixed. I have a diagram of a pup with > lines > > through it showing me where to take my sections from. This > ensures > > consistency from pup to pup. I just process the pups on my > normal > > processing schedule and have had great results from this. > > > > Feel free to email me if you have any questions about this or > would like > > me to send you the pup diagram of where to take sections from. > > > > Good luck > > > > Derek > > > > -- > > Derek Papalegis HT (ASCP) > > Histotechnician > > Division of Laboratory Animal Medicine > > Tufts University > > 136 Harrison Avenue > > Boston, MA 02111 > > phone: 617 636-2971 > > fax: 617 636-8354 > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From amosbrooks <@t> gmail.com Sat Feb 9 22:16:06 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sat Feb 9 22:16:09 2008 Subject: [Histonet] Mouse pups and processing Message-ID: <582736990802092016r5b9a9091wc1bfebdbaaae20ca@mail.gmail.com> Pam, We were having a similar problem with one of our researcher's P0 mice. I don't know how they sacrificed them, but they too dod not process at all. They were squishy! I reccommended they either bisect or at least make a good midline incision prior to fixation. It still isn't perfect (is it ever?), but it seems to be a bit better. This allows better solution penetration. Give it a whirl and let us know how it goes. Amos Message: 13 Date: Fri, 08 Feb 2008 15:52:34 -0500 From: Pamela Marcum Subject: [Histonet] Mouse pups and processing To: Histonet@lists.utsouthwestern.edu Message-ID: <6.2.5.6.2.20080208154217.01c8a9b8@vet.upenn.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed I have been struggling with this for a while and need some help. We currently have a project with 0 day mouse pups that are allowed to be born normally and then sacrificed with CO2. We had several groups earlier that were sacrificed a different way and they processed and sectioned beautifully. These don't seem to fix well, dehydrate, clear or infiltrate worth a darn. Since this is my first time using CO2 for sacrifice I need to find out if it causes a problem or if I am just losing it. I have not ever had this problem before and even re-processing does not help. I know they are not infiltrating as they are floating in paraffin at the end if I remove them from the processor to a vat of paraffin. They will not sink. The pups are 1.2 to 1.3 grams each. If you can suggest a better way to sacrifice them please let me know. Killing the mother and perfusing her is not an option as these are not our mice. They are being given as favor so I am limited to some extent. This was an overnight process with slightly altered alcohols to 45 minutes each, 1 xylene at 30 minutes and 2 Shandon Xylene Substitues at 10 hour each to 4 paraffins at 45 X2 and 1 hour X2. Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From pmarcum <@t> vet.upenn.edu Sun Feb 10 07:57:55 2008 From: pmarcum <@t> vet.upenn.edu (pmarcum@vet.upenn.edu) Date: Sun Feb 10 07:58:01 2008 Subject: [Histonet] Re: Mouse pups and processing In-Reply-To: <582736990802092016r5b9a9091wc1bfebdbaaae20ca@mail.gmail.com> References: <582736990802092016r5b9a9091wc1bfebdbaaae20ca@mail.gmail.com> Message-ID: <1202651875.47af02e3486c9@imp.vet.upenn.edu> Thank you Amos and I can appreciate your problem. Your discription of sqishy is prefect. I have also referred to the outer skin as lacy in appearance when attempting to section it. The other point I would make is the brain is mush with no form as if it is autolysized totally. I have tried splitting the abdomen and it does not help or is so minimal it doesn't help much. I have tried changing the processor and always process other tissues at the same time to show the PI it is not the reagents and paraffin. We have processed other pups before this over the last year and know they were not killed with CO2. They work great and section great (I still split the abdomen). I am at a loss and finally turned to all of you on HistoNet help. You are great and even though we may not have the answer yet we will I am sure of it. I know this is an ongoing problem for researchers with pups. Maybe with this CO2 thing being a popular way for sacrificing animals we have hit on something that happens in pups and not adults. I had not really ever worried about it until now as I did my own sacrifice and it was fine. I wish they weren't so small I would attempt prefusion with a 2cc syringe and blunt needle as a last resort. I want to THANK everyone who has and will take the time to answer and help me with this issue. At least I can show the PI our lab is not the only one with the problem and we are all attempting to find a way out of it. I think from all I have seen we should be more aware of how the pups are sacrificed and note any differences between the way our PI's sacrifice them. Maybe all of the material submitted to the Histology area for research on pups and other animals should be recorded and we might see differences they don't in method. Recommendations from IACOCC do not take our area of histology into consideration as they are controlling the most humane and painless method of sacrificing animals over all. Perhaps one of us can get a paper out of this or a talk in the future that helps everyone. Who knows I even checked the phase of the moon this last time and it did not help. Thanks again and still looking. If I solve this you will all be teh first to know. Pam Quoting Amos Brooks : > Pam, > We were having a similar problem with one of our researcher's P0 mice. > I don't know how they sacrificed them, but they too dod not process at all. > They were squishy! > I reccommended they either bisect or at least make a good midline > incision prior to fixation. It still isn't perfect (is it ever?), but it > seems to be a bit better. This allows better solution penetration. Give it a > whirl and let us know how it goes. > > Amos > > Message: 13 > Date: Fri, 08 Feb 2008 15:52:34 -0500 > From: Pamela Marcum > Subject: [Histonet] Mouse pups and processing > To: Histonet@lists.utsouthwestern.edu > Message-ID: <6.2.5.6.2.20080208154217.01c8a9b8@vet.upenn.edu> > Content-Type: text/plain; charset="us-ascii"; format=flowed > > > > I have been struggling with this for a while and need some help. We > currently have a project with 0 day mouse pups that are allowed to be > born normally and then sacrificed with CO2. We had several groups > earlier that were sacrificed a different way and they processed and > sectioned beautifully. > > These don't seem to fix well, dehydrate, clear or infiltrate worth a > darn. Since this is my first time using CO2 for sacrifice I need to > find out if it causes a problem or if I am just losing it. I have > not ever had this problem before and even re-processing does not > help. I know they are not infiltrating as they are floating in > paraffin at the end if I remove them from the processor to a vat of > paraffin. They will not sink. The pups are 1.2 to 1.3 grams each. > > If you can suggest a better way to sacrifice them please let me > know. Killing the mother and perfusing her is not an option as these > are not our mice. They are being given as favor so I am limited to > some extent. > > This was an overnight process with slightly altered alcohols to 45 > minutes each, 1 xylene at 30 minutes and 2 Shandon Xylene Substitues > at 10 hour each to 4 paraffins at 45 X2 and 1 hour X2. > > Best Regards, > > Pamela A Marcum > Manager, Histology Special Procedures > University of Pennsylvania > School of Veterinary Medicine > R.S. Reynolds Jr. CORL > New Bolton Center > 382 West Street Road > Kennett Square, PA 19348 > > Phone - 610-925-6278 > Fax - 610-925-8120 > E-mail - pmarcum@vet.upenn.edu > From gvdobbin <@t> ihis.org Mon Feb 11 06:54:11 2008 From: gvdobbin <@t> ihis.org (Greg Dobbin) Date: Mon Feb 11 06:54:43 2008 Subject: Fwd: [Histonet] Re: Mouse pups and processing Message-ID: Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 There is some merit in doing the right thing rather badly, but absolutely none in doing the wrong thing excellently! >>> Greg Dobbin 2/11/2008 8:53 AM >>> Hi Pam, I participated on a project about 10 years ago where the rat pups were anesthetized using an overdose of halothane followed by exanguinationn (carotid and/or renal arteries). Here are the 2 relevant references: Comp Med. 2001 Apr;51(2):134-7. Histol Histopathol. 2002 Oct;17(4):1067-76. Good luck. Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 There is some merit in doing the right thing rather badly, but absolutely none in doing the wrong thing excellently! >>> 2/10/2008 9:57 AM >>> Thank you Amos and I can appreciate your problem. Your discription of sqishy is prefect. I have also referred to the outer skin as lacy in appearance when attempting to section it. The other point I would make is the brain is mush with no form as if it is autolysized totally. I have tried splitting the abdomen and it does not help or is so minimal it doesn't help much. I have tried changing the processor and always process other tissues at the same time to show the PI it is not the reagents and paraffin. We have processed other pups before this over the last year and know they were not killed with CO2. They work great and section great (I still split the abdomen). I am at a loss and finally turned to all of you on HistoNet help. You are great and even though we may not have the answer yet we will I am sure of it. I know this is an ongoing problem for researchers with pups. Maybe with this CO2 thing being a popular way for sacrificing animals we have hit on something that happens in pups and not adults. I had not really ever worried about it until now as I did my own sacrifice and it was fine. I wish they weren't so small I would attempt prefusion with a 2cc syringe and blunt needle as a last resort. I want to THANK everyone who has and will take the time to answer and help me with this issue. At least I can show the PI our lab is not the only one with the problem and we are all attempting to find a way out of it. I think from all I have seen we should be more aware of how the pups are sacrificed and note any differences between the way our PI's sacrifice them. Maybe all of the material submitted to the Histology area for research on pups and other animals should be recorded and we might see differences they don't in method. Recommendations from IACOCC do not take our area of histology into consideration as they are controlling the most humane and painless method of sacrificing animals over all. Perhaps one of us can get a paper out of this or a talk in the future that helps everyone. Who knows I even checked the phase of the moon this last time and it did not help. Thanks again and still looking. If I solve this you will all be teh first to know. Pam Quoting Amos Brooks : > Pam, > We were having a similar problem with one of our researcher's P0 mice. > I don't know how they sacrificed them, but they too dod not process at all. > They were squishy! > I reccommended they either bisect or at least make a good midline > incision prior to fixation. It still isn't perfect (is it ever?), but it > seems to be a bit better. This allows better solution penetration. Give it a > whirl and let us know how it goes. > > Amos > > Message: 13 > Date: Fri, 08 Feb 2008 15:52:34 -0500 > From: Pamela Marcum > Subject: [Histonet] Mouse pups and processing > To: Histonet@lists.utsouthwestern.edu > Message-ID: <6.2.5.6.2.20080208154217.01c8a9b8@vet.upenn.edu> > Content-Type: text/plain; charset="us-ascii"; format=flowed > > > > I have been struggling with this for a while and need some help. We > currently have a project with 0 day mouse pups that are allowed to be > born normally and then sacrificed with CO2. We had several groups > earlier that were sacrificed a different way and they processed and > sectioned beautifully. > > These don't seem to fix well, dehydrate, clear or infiltrate worth a > darn. Since this is my first time using CO2 for sacrifice I need to > find out if it causes a problem or if I am just losing it. I have > not ever had this problem before and even re-processing does not > help. I know they are not infiltrating as they are floating in > paraffin at the end if I remove them from the processor to a vat of > paraffin. They will not sink. The pups are 1.2 to 1.3 grams each. > > If you can suggest a better way to sacrifice them please let me > know. Killing the mother and perfusing her is not an option as these > are not our mice. They are being given as favor so I am limited to > some extent. > > This was an overnight process with slightly altered alcohols to 45 > minutes each, 1 xylene at 30 minutes and 2 Shandon Xylene Substitues > at 10 hour each to 4 paraffins at 45 X2 and 1 hour X2. > > Best Regards, > > Pamela A Marcum > Manager, Histology Special Procedures > University of Pennsylvania > School of Veterinary Medicine > R.S. Reynolds Jr. CORL > New Bolton Center > 382 West Street Road > Kennett Square, PA 19348 > > Phone - 610-925-6278 > Fax - 610-925-8120 > E-mail - pmarcum@vet.upenn.edu > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. D?claration de confidentialit? Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ayant pour objet une personne ou un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer compl?tement de votre syst?me informatique. From karenadams <@t> comcast.net Mon Feb 11 08:17:37 2008 From: karenadams <@t> comcast.net (karenadams@comcast.net) Date: Mon Feb 11 08:17:47 2008 Subject: [Histonet] Verhoeff/ Trichome procedure Message-ID: <021120081417.12064.47B05901000102CF00002F2022070029539C030E0B0E020A9D0E05@comcast.net> Does anyone have a procedure for Verhoeff's-Masson Trichrome they would be willing to share? Thank you in advance :) -- Karen Adams Supervisor Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 From failm <@t> musc.edu Mon Feb 11 08:58:51 2008 From: failm <@t> musc.edu (Fail, Mildred M.) Date: Mon Feb 11 08:59:31 2008 Subject: [Histonet] Verhoeff/ Trichome procedure In-Reply-To: <021120081417.12064.47B05901000102CF00002F2022070029539C030E0B0E020A9D0E05@comcast.net> References: <021120081417.12064.47B05901000102CF00002F2022070029539C030E0B0E020A9D0E05@comcast.net> Message-ID: ACTION DATE INITIALS MODIFIED MASSON?S PURPOSE: To identify elastic in addition to connective tissue (collagen and muscle). PRINCIPLES: Masson's Modified is the combination of Masson's Trichrome and Verhoeff's Van Gieson (VVG) stains. The stain procedure is the same as Masson's Trichrome with the addition of a VVG procedure to stain elastic fibers. After the elastic fibers are stained, we continue on with the routine Masson's Trichrome stain. FIXATIVES: 10% neutral buffered formalin, B-3, B-5, Zenker?s, or Bouin?s. EQUIPMENT: Waterbath, Coplin jars, pH meter, Erlenmeyer flasks, graduated cylinders, filter paper. TECHNIQUE: Cut paraffin sections at 2 to 5?. QUALITY CONTROL: A section of small intestine, appendix, or colon should be used as a positive control. PRECAUTIONS: Follow laboratory safety precautions: Labcoat, gloves, glasses, etc. Gloves are necessary to avoid absorption of dyes into skin. All acids are corrosive. Avoid inhaling dye powders as synthetic dyes may cause neoplasms. Exercise caution when hamdling hot olutions. Picric acid is unstable explosive if in the dry state, keep wet. Formalin is poison, may cause cancer. Fumes are hazardous, it is harmful if inhaled or absorbed through skin. Ferric chloride, phosphomolybdic acid and acetic acid are corrosive Dispose of hazardous waste in appropriate receptacles. SOLUTIONS: SATURATED PICRIC ACID Available from Polyscientific BOUIN'S FLUID Saturated Picric Acid 75.0 ml Formalin, Full Strength 25.0 ml Glacial Acetic Acid 5.0 ml 5% ALCOHOLIC HEMATOXYLIN 100% Ethanol 100.0 ml Hematoxylin 5.0 gm 10% FERRIC CHLORIDE Distilled Water 200.0 ml Ferric Chloride 20.0 gm VERHOEFF'S IODINE Distilled Water 100.0 ml Iodine 2.0 gm Potassium Iodate 4.0 gm VERHOEFF'S ELASTIC TISSUE STAIN 5% Alcoholic Hematoxylin 22.0 ml 10% Ferric Chloride 8.0 ml Verhoeff's Iodine 8.0 ml Prepare fresh and use the same day. 1% BIEBRICH SCARLET Distilled Water 100.0 ml Biebrich Scarlet 1.0 gm 1% ACID FUCHSIN Distilled Water 100.0 ml Acid Fuchsin 1.0 gm BIEBRICH SCARLET-ACID FUCHSIN SOLUTION 1% Biebrich Scarlet 45.0 ml 1% Acid Fuchsin 5.0 ml Glacial Acetic Acid 0.5 ml Mix and filter to prevent red staining artifact. PHOSPHOMOLYBDIC-PHOSPHOTUNGSTIC ACID SOLUTION Distilled Water 100.0 ml Phosphomolybdic Acid 2.5 gm Phosphotungstic Acid 2.5 gm ANILINE BLUE SOLUTION Distilled Water 100.0 ml Aniline Blue 2.5 gm Glacial Acetic Acid 2.0 ml 1% GLACIAL ACETIC ACID Distilled water 99.0 ml Glacial Acetic Acid 1.0 ml STAINING PROCEDURE: Preheat the Bouin?s in a 60? water bath for at least 10 minutes 1. Hydrate slides to distilled water. 2. Mordant slides in preheated Bouin's fluid in a 60? water bath for 5 minutes 3. Wash in water until all yellow is removed. 4. Verhoeff's Elastic Tissue Stain for 20 minutes. 5. Rinse well in water. 6. Differentiate in diluted 2% Ferric Chloride. Elastic fibers should be black, nuclei black, and other tissue elements dark gray. 7. Rinse well in distilled water. 8. 95% Alcohol for 1 minute. 9. Rinse in distilled water. 10. Biebrich Scarlet-Acid Fuchsin Solution for 2 minutes. 11. Rinse in distilled water. 12. Phosphomolybdic-Phosphotungstic Acid for 20 minutes. USE FRESH. DO NOT RINSE! 13. Aniline Blue for 10 minutes. Rinse in distilled water. 14. 1% Glacial Acetic Acid for 3 minutes to blue. 15. Rinse in distilled water. 16. Dehydrate, clear, and mount. RESULTS: Nuclei and Elastic Fibers - Blue-Black to Black Keratin and Muscle Fibers - Red Collagen - Blue REFERENCES: Sheehan, D. and Hrapchak, B., Theory and Practice of Histotechnology, 2nd Edition. St. Louis: The C.V. Mosby Company, 1980. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of karenadams@comcast.net [karenadams@comcast.net] Sent: Monday, February 11, 2008 9:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Verhoeff/ Trichome procedure Does anyone have a procedure for Verhoeff's-Masson Trichrome they would be willing to share? Thank you in advance :) -- Karen Adams Supervisor Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 FAX (865) 691-1623 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TillRenee <@t> uams.edu Mon Feb 11 08:59:18 2008 From: TillRenee <@t> uams.edu (Till, Renee) Date: Mon Feb 11 08:59:57 2008 Subject: [Histonet] Iron staining Message-ID: <11F927674DEBDC43B960809A7403C5D2075A8F05@MAILPED.ad.uams.edu> Okay, a few questions/concerns about iron staining. I am trying to help a tech who has never done histology before, do the Prussian blue and Turnbull stains for iron. These should detect the ferrous or ferric iron in most any tissues that contain it correct? The PI wants to see what, if anything shows up in some rat jejunum sections. She's only run one test run with liver as a control. This is our first go a iron stains, so we weren't sure about what tissue to use as a control. I was going to have her try spleen since neither the liver or the gi tissues worked. Of course we are assuming that the stain worked as it should anyway. So for someone starting fresh, what tissue would you suggest as a possible control? We don't have any tissue that we know contains hematochromatosis or hemosiderosis like my books say. Another tech took it upon himself to do some research and found a paper that has now made them think that these stains only work on tissue with conditions like these. Am I correct in thinking that this is not true, only that these have more iron for the stain to detect? Renee' Till, HT (ASCP) Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72202 Lab (501)364-8504 Office Fax (501)364-3161 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From CIngles <@t> uwhealth.org Mon Feb 11 11:10:17 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Mon Feb 11 11:13:48 2008 Subject: [Histonet] OT: the weather References: <000101c86a5e$43c08660$d00f7ca5@lurie.northwestern.edu> <004901c86aab$342a5300$0302a8c0@yourxhtr8hvc4p> Message-ID: <08A0A863637F1349BBFD83A96B27A50A1200E5@uwhis-xchng3.uwhis.hosp.wisc.edu> Hey Joe: A wize mom once told me that if we didn't have weather, there would be nothing to gripe about. (I'm sure we could find something else, but who knows.) p.s. Ya ever hear of ice cores and tree rings? :) It's Friday and I haven't been flamed in a while. Weather records have been around for what? 100 years or so? How old is our planet, millions of years? So you think that 100 years of data can be spread across millions of years? Here's a new thought. What if this is just a natural occasion between ice ages? JTT From jcline <@t> wchsys.org Mon Feb 11 11:35:21 2008 From: jcline <@t> wchsys.org (Joyce Cline) Date: Mon Feb 11 11:35:34 2008 Subject: [Histonet] prostate biopsy problem Message-ID: <000c01c86cd4$7b41b730$1d2a14ac@wchsys.org> We are having problems with our prostate biopsies washing off the slide. We have used Superfrost + slides, stay on in the water bath, drying until the tissue was opaque and extra heating time in the dryer. We have run out of ideas. We are thinking of going to H&E hand staining just for the prostates. ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From Lynne.Bell <@t> hitchcock.org Mon Feb 11 12:17:17 2008 From: Lynne.Bell <@t> hitchcock.org (Bell, Lynne) Date: Mon Feb 11 12:17:23 2008 Subject: [Histonet] prostate biopsy problem In-Reply-To: <000c01c86cd4$7b41b730$1d2a14ac@wchsys.org> Message-ID: We had the same problem a few years ago. Do you use sponges in your cassette? We started using wet sponges instead of dry ones. We have not had a problem since. Lynne Bell, HT (ASCP) Central VT Hospital P. O. Box 547 Barre, VT 05641 802-371-4923 From jcline <@t> wchsys.org Mon Feb 11 12:52:16 2008 From: jcline <@t> wchsys.org (Joyce Cline) Date: Mon Feb 11 12:52:22 2008 Subject: [Histonet] re/prostate quesitons Message-ID: <000701c86cdf$392aeb40$1d2a14ac@wchsys.org> 1. We do not have the same problem with our breast core biopsies. 2. We may have washing on block A and B but not on C,D or F and washing on E, H, etc. 3. The biopsies have no drying artifact on the edges. 4. We use a low temp wax, Blue Ribbon paraffin for processing and embedding. Our processor is set at 57. 5. The tissue sections fall off during H&E staining. 6. We do not use sponges, we use screened cassettes. 7. The Stay on is used alone, not with the charged slides. Our pathologist that is our prostate specialist thinks each one of us is doing something different when we cut. We have all discussed this matter and can find no differences when we cut. ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From mohs76009 <@t> yahoo.com Mon Feb 11 13:17:43 2008 From: mohs76009 <@t> yahoo.com (Matt Bancroft) Date: Mon Feb 11 13:17:48 2008 Subject: [Histonet] re/prostate quesitons In-Reply-To: <000701c86cdf$392aeb40$1d2a14ac@wchsys.org> Message-ID: <446182.81960.qm@web63409.mail.re1.yahoo.com> The issue that I have found is even when one uses + charged slides, you still need to make sure that all the water is gone from under the tissue section. When I cut needle biopsies (kidney, prostate, or breast) after I get the section on the slide I take my forceps tare a little slit in the corner of my sections and use a kim wipe to absorb the water. I have not had a problem with sections falling off since I started doing this. I also use HISTOBOND slides from StatLab medical products. These slides are very sticky (sometimes to much). Matt Bancroft HT(ASCP) Laboratory Manager Joyce Cline wrote: 1. We do not have the same problem with our breast core biopsies. 2. We may have washing on block A and B but not on C,D or F and washing on E, H, etc. 3. The biopsies have no drying artifact on the edges. 4. We use a low temp wax, Blue Ribbon paraffin for processing and embedding. Our processor is set at 57. 5. The tissue sections fall off during H&E staining. 6. We do not use sponges, we use screened cassettes. 7. The Stay on is used alone, not with the charged slides. Our pathologist that is our prostate specialist thinks each one of us is doing something different when we cut. We have all discussed this matter and can find no differences when we cut. ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From jennifer.l.hofecker <@t> Vanderbilt.Edu Mon Feb 11 13:25:00 2008 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Mon Feb 11 13:25:07 2008 Subject: [Histonet] Tennessee Society for Histotechnology - Call for Vendors Message-ID: <898D946569A27444B65667A49C0740520156449C@mailbe06.mc.vanderbilt.edu> Happy Monday to all. I just wanted to take a moment and post a call for vendors. The TSH meeting is June 19-21 in Townsend, TN. Townsend is located in the scenic Smoky Mountain area. The meeting will be held at the valley view lodge. If you are interested in exhibiting at our meeting, please contact me for complete information. The deadline to have your company listed in the program as a sponsor is March 1. The official programs should be mailed by April 1 and I will post contact info again at that time for anyone interested in attending the meeting who may not receive a program. Thanks and have a great week! Jennifer L. Hofecker, HT(ASCP) Vanderbilt University Medical Center Division of Neuropathology Nashville, TN ph. (615)343-0083 fax. (615)343-7089 NSH Quality Control Committee Chair Tennessee Society for Histotechnology Secretary 2008 TSH Meeting Exhibit Coordinator From jcline <@t> wchsys.org Mon Feb 11 13:31:41 2008 From: jcline <@t> wchsys.org (Joyce Cline) Date: Mon Feb 11 13:31:46 2008 Subject: [Histonet] re/prostate questions Message-ID: <000701c86ce4$ba8c4f80$1d2a14ac@wchsys.org> Our slides are dried vertically on plastic slide racks. I do not use Xylene, I use Formula 83. ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From asachau <@t> titanmed.com Mon Feb 11 14:06:16 2008 From: asachau <@t> titanmed.com (April Sachau) Date: Mon Feb 11 14:09:06 2008 Subject: [Histonet] $$$ Histology Position $$$ In-Reply-To: <898D946569A27444B65667A49C0740520156449C@mailbe06.mc.vanderbilt.edu> Message-ID: <7E3ACD48BA6E26408F3188FBF08693F7010D4C56@titansbs1.corp.titanmed.com> Hello Histonetters! I have an awesome position available in the Midwest. Great compensation package!!! It is a dayshift position for a minimum of 13 weeks. Possible permanent placement! Please let me know if you are interested or would like additional information! April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com From Pathrm35 <@t> comcast.net Mon Feb 11 16:31:07 2008 From: Pathrm35 <@t> comcast.net (Pathrm35@comcast.net) Date: Mon Feb 11 16:31:15 2008 Subject: [Histonet] Phoenix AZ histotechs Message-ID: <021120082231.3815.47B0CCAA000F06C100000EE72215551724CACC039D089B0EAF@comcast.net> Could a histotech from the Phoenix, AZ area please contact me? Thanks, Ron Martin From jonesly <@t> mir.wustl.edu Mon Feb 11 17:03:12 2008 From: jonesly <@t> mir.wustl.edu (Jones, Lynne) Date: Mon Feb 11 17:03:43 2008 Subject: [Histonet] RE: Histonet Digest, mouse pup euthanasia with CO2 In-Reply-To: <078368b7-9ead-4bb7-b00a-e4cd2501b722> References: <078368b7-9ead-4bb7-b00a-e4cd2501b722> Message-ID: Hello to the list - I have little histology experience, but I've dealt with complications arising from both anesthesia and euthanasia in lab animals. Standard protocols don't work for every model. Neonatal rodents are very resistant to hypoxia (death can take up to 50 minutes, depending on age and strain). Our institutional policy is that neonatal rats and mice must remain inside the CO2 chamber for a minimum of 20 minutes after the gas flow is turned off if CO2 is the sole agent used for euthanasia. Many Institutional Animal Care and Use Committees suggest consideration of alternative methods for neonates, or that CO2 anesthesia be followed by a physical method. FWIW, the paper below describes some of the biological effects associated with different euthanasia techniques. http://www.aclam.org/print/report_rodent_euth.pdf Based on the description of the brains, my suspicion is that the pups sat too long post-mortem before being fixed, or (going way out on a limb) that acidosis might have created problems if the formalin wasn't buffered. Lynne Jones -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, February 11, 2008 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 51, Issue 17 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Fwd: [Histonet] Re: Mouse pups and processing (Greg Dobbin) 2. Verhoeff/ Trichome procedure (karenadams@comcast.net) 3. RE: Verhoeff/ Trichome procedure (Fail, Mildred M.) 4. Iron staining (Till, Renee) 5. RE: OT: the weather (Ingles Claire) 6. prostate biopsy problem (Joyce Cline) ---------------------------------------------------------------------- Message: 1 Date: Mon, 11 Feb 2008 08:54:11 -0400 From: "Greg Dobbin" Subject: Fwd: [Histonet] Re: Mouse pups and processing To: Message-ID: Content-Type: text/plain; charset=US-ASCII Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 There is some merit in doing the right thing rather badly, but absolutely none in doing the wrong thing excellently! >>> Greg Dobbin 2/11/2008 8:53 AM >>> Hi Pam, I participated on a project about 10 years ago where the rat pups were anesthetized using an overdose of halothane followed by exanguinationn (carotid and/or renal arteries). Here are the 2 relevant references: Comp Med. 2001 Apr;51(2):134-7. Histol Histopathol. 2002 Oct;17(4):1067-76. Good luck. Greg Greg Dobbin, R.T. Chief Technologist, Histology Lab Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 There is some merit in doing the right thing rather badly, but absolutely none in doing the wrong thing excellently! >>> 2/10/2008 9:57 AM >>> Thank you Amos and I can appreciate your problem. Your discription of sqishy is prefect. I have also referred to the outer skin as lacy in appearance when attempting to section it. The other point I would make is the brain is mush with no form as if it is autolysized totally. I have tried splitting the abdomen and it does not help or is so minimal it doesn't help much. I have tried changing the processor and always process other tissues at the same time to show the PI it is not the reagents and paraffin. We have processed other pups before this over the last year and know they were not killed with CO2. They work great and section great (I still split the abdomen). I am at a loss and finally turned to all of you on HistoNet help. You are great and even though we may not have the answer yet we will I am sure of it. I know this is an ongoing problem for researchers with pups. Maybe with this CO2 thing being a popular way for sacrificing animals we have hit on something that happens in pups and not adults. I had not really ever worried about it until now as I did my own sacrifice and it was fine. I wish they weren't so small I would attempt prefusion with a 2cc syringe and blunt needle as a last resort. I want to THANK everyone who has and will take the time to answer and help me with this issue. At least I can show the PI our lab is not the only one with the problem and we are all attempting to find a way out of it. I think from all I have seen we should be more aware of how the pups are sacrificed and note any differences between the way our PI's sacrifice them. Maybe all of the material submitted to the Histology area for research on pups and other animals should be recorded and we might see differences they don't in method. Recommendations from IACOCC do not take our area of histology into consideration as they are controlling the most humane and painless method of sacrificing animals over all. Perhaps one of us can get a paper out of this or a talk in the future that helps everyone. Who knows I even checked the phase of the moon this last time and it did not help. Thanks again and still looking. If I solve this you will all be teh first to know. Pam Quoting Amos Brooks : > Pam, > We were having a similar problem with one of our researcher's P0 mice. > I don't know how they sacrificed them, but they too dod not process at all. > They were squishy! > I reccommended they either bisect or at least make a good midline > incision prior to fixation. It still isn't perfect (is it ever?), but it > seems to be a bit better. This allows better solution penetration. Give it a > whirl and let us know how it goes. > > Amos > > Message: 13 > Date: Fri, 08 Feb 2008 15:52:34 -0500 > From: Pamela Marcum > Subject: [Histonet] Mouse pups and processing > To: Histonet@lists.utsouthwestern.edu > Message-ID: <6.2.5.6.2.20080208154217.01c8a9b8@vet.upenn.edu> > Content-Type: text/plain; charset="us-ascii"; format=flowed > > > > I have been struggling with this for a while and need some help. We > currently have a project with 0 day mouse pups that are allowed to be > born normally and then sacrificed with CO2. We had several groups > earlier that were sacrificed a different way and they processed and > sectioned beautifully. > > These don't seem to fix well, dehydrate, clear or infiltrate worth a > darn. Since this is my first time using CO2 for sacrifice I need to > find out if it causes a problem or if I am just losing it. I have > not ever had this problem before and even re-processing does not > help. I know they are not infiltrating as they are floating in > paraffin at the end if I remove them from the processor to a vat of > paraffin. They will not sink. The pups are 1.2 to 1.3 grams each. > > If you can suggest a better way to sacrifice them please let me > know. Killing the mother and perfusing her is not an option as these > are not our mice. They are being given as favor so I am limited to > some extent. > > This was an overnight process with slightly altered alcohols to 45 > minutes each, 1 xylene at 30 minutes and 2 Shandon Xylene Substitues > at 10 hour each to 4 paraffins at 45 X2 and 1 hour X2. > > Best Regards, > > Pamela A Marcum > Manager, Histology Special Procedures > University of Pennsylvania > School of Veterinary Medicine > R.S. Reynolds Jr. CORL > New Bolton Center > 382 West Street Road > Kennett Square, PA 19348 > > Phone - 610-925-6278 > Fax - 610-925-8120 > E-mail - pmarcum@vet.upenn.edu The materials in this message are private and may contain Protected Healthcare Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return mail. From nickandmanda <@t> paradise.net.nz Tue Feb 12 01:30:24 2008 From: nickandmanda <@t> paradise.net.nz (Nick and Amanda) Date: Tue Feb 12 01:30:33 2008 Subject: [Histonet] new prostate problem! Message-ID: <000d01c86d49$21db4ec0$c8084979@bow1> Hi! We have recently come up against a staining issue where our prostate cores have patches which are not staining. Our Pathologist tells us this is common in a particular High grade diagnosis so this is a problem when it is happening in all prostate cases.. It does not seem to be affecting other tissue staining (perhaps the odd one! no examples could be provided!! Could it possibly be drying out of the cores prior to being placed in formalin?? Any Ideas welcome Just not sure! Wellington, NZ From relia1 <@t> earthlink.net Tue Feb 12 07:14:57 2008 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Feb 12 07:15:02 2008 Subject: [Histonet] RELIA Histology Job Opportunity Alert 2/12/08 Message-ID: Hi Histonetters!! Just wanted to do a quick post on some of the jobs I am currently working on. These clients are all looking for ASCP certified or eligible people interested in full time dayshift permanent positions. They offer excellent compensation, benefits and relocation assistance if needed. If you or someone you know is interested please contact me and please feel free to pass this information along to others as well Here is a list of the locations where I have some great opportunities: Texas - Multiple locations management and tech positions South Carolina - tech position Washington - management and tech positions Montana - tech position Pennsylvania - multiple locations tech positions New Hampshire - tech position California - tech positions - ASCP certification is required Virginia - tech position Remember it never hurts to look. You can reach me at 866-60-RELIA (866-607-3542) or at relia1@earthlink.net Have a Happy Valentines Day!!! Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll Free(866)-607-3542 Fax: (407)678-2788 E-mail: relia1@earthlink.net www.myspace.com/pamatrelia From Sharon.Davis-Devine <@t> carle.com Tue Feb 12 08:11:49 2008 From: Sharon.Davis-Devine <@t> carle.com (Sharon.Davis-Devine) Date: Tue Feb 12 08:11:56 2008 Subject: [Histonet] Formalin recycler Message-ID: <44780C571F28624DBB446DE55C4D733A021E08BE@EXCHANGEBE1.carle.com> Presently we have two CBG recycling machines; one recycles alcohol and the other formalin. We seem to always be backed up on formalin because it takes so long to run a cycle. Is there some other system out there that offers a faster turn around time? Any suggestions would be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com From laurie.colbert <@t> huntingtonhospital.com Tue Feb 12 09:27:26 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Tue Feb 12 09:27:35 2008 Subject: [Histonet] Formalin recycler Message-ID: <57BE698966D5C54EAE8612E8941D76830267DD39@EXCHANGE3.huntingtonhospital.com> Creative Waste Solutions has a formalin recycler that is very fast and easy to use. We love it! We use our CBG recycler for alcohol and xylene and the Creative Waste recycler for the formalin. They can be reached at (888) 795-8300. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Tuesday, February 12, 2008 6:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin recycler Presently we have two CBG recycling machines; one recycles alcohol and the other formalin. We seem to always be backed up on formalin because it takes so long to run a cycle. Is there some other system out there that offers a faster turn around time? Any suggestions would be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charlene.Henry <@t> STJUDE.ORG Tue Feb 12 09:59:45 2008 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Tue Feb 12 09:59:50 2008 Subject: [Histonet] Opening for a Core Lab Manager Message-ID: <03E1F5968F60C5448635D49D38B283ED03F507BF@SJMEMXMBS11.stjude.sjcrh.local> We currently have an opening for a Histology Core Lab Manager in our new Histology Core Lab that is due to be completed within the next couple of months. If you are interested, you can send me an email or visit www.stjue.org. I'll have to say that St Jude is one of the greatest places I have ever worked and would recommend this position to anyone looking for a research lab to manage. Thanks, Charlene Henry HT (ASCP), QIHC Anatomic Pathology Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 From pkromund <@t> gundluth.org Tue Feb 12 10:29:55 2008 From: pkromund <@t> gundluth.org (pkromund@gundluth.org) Date: Tue Feb 12 10:30:06 2008 Subject: [Histonet] new prostate problem! In-Reply-To: <000d01c86d49$21db4ec0$c8084979@bow1> Message-ID: Yes, drying prior to being placed in formalin does have adverse affects on staining. Nick and Amanda To Sent by: histonet@lists.utsouthwestern.edu histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] new prostate problem! 02/12/2008 01:30 AM Hi! We have recently come up against a staining issue where our prostate cores have patches which are not staining. Our Pathologist tells us this is common in a particular High grade diagnosis so this is a problem when it is happening in all prostate cases.. It does not seem to be affecting other tissue staining (perhaps the odd one! no examples could be provided!! Could it possibly be drying out of the cores prior to being placed in formalin?? Any Ideas welcome Just not sure! Wellington, NZ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charlene.Henry <@t> STJUDE.ORG Tue Feb 12 10:48:00 2008 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Tue Feb 12 11:01:01 2008 Subject: [Histonet] Histology Core Lab Manager Position Message-ID: <03E1F5968F60C5448635D49D38B283ED03F507C0@SJMEMXMBS11.stjude.sjcrh.local> In my earlier email, I left out part of the email address and it is not even Monday. The correct link is www.stjude.org; sorry for the confusion. Thanks, Charlene Henry HT (ASCP), QIHC Anatomic Pathology Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 From Charlene.Henry <@t> STJUDE.ORG Tue Feb 12 10:59:16 2008 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Tue Feb 12 11:01:11 2008 Subject: [Histonet] Histology Core Lab Manager Position Message-ID: <03E1F5968F60C5448635D49D38B283ED03F507C1@SJMEMXMBS11.stjude.sjcrh.local> If interested in this position go to www.stjude.org and under "Jobs" the position # is 17078 and the title of this position is "Laboratory Manager Veterinary Histology Core". Thanks, Charlene Henry HT (ASCP), QIHC Anatomic Pathology Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 From Heather.D.Renko <@t> osfhealthcare.org Tue Feb 12 12:04:20 2008 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Tue Feb 12 12:04:39 2008 Subject: [Histonet] Re: Used equipment Message-ID: <40026EDDE64CDA47AB382C52619ACD3C0A09DD2B@pmc-rfd-mx01.intranet.osfnet.org> I have a Microm 760X H&E stainer that we are replacing. If you know of anyone that might want it or even for parts email me directly. Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From cormier <@t> MIT.EDU Tue Feb 12 13:21:41 2008 From: cormier <@t> MIT.EDU (Kathy Cormier) Date: Tue Feb 12 13:21:48 2008 Subject: [Histonet] ? vimentin IHC ab from ABR bioreagents Message-ID: <000a01c86dac$7f5fb3c0$92003712@mit.edu> Hi All, Has anyone tried the vimetin mouse mono ab from ABR bioreagents? I need to find a vimentin that will work on FFPE mouse tissue, and I do not want to spend $300 if the ab does not work well.... Any other ab that anyone knows works well? (yes, I checked the archives!) Thanks! Kathy DCM MIT From phineline <@t> grandpath.com Tue Feb 12 14:02:29 2008 From: phineline <@t> grandpath.com (Hineline, Paula) Date: Tue Feb 12 14:01:01 2008 Subject: [Histonet] H & E stainers Message-ID: Hello, We are researching histology H&E stainers for our lab. We have gotten quotes on the Leica ST 5020 multistainer and the Leica ST 4040 linear stainer. Recommendations would be appreciated. Thanks Paula Hineline, HT ASCP Pathology Laboratory phineline@grandpath.com From POWELL_SA <@t> Mercer.edu Tue Feb 12 14:17:13 2008 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Tue Feb 12 14:21:20 2008 Subject: [Histonet] Region III Meeting Program Message-ID: <01MR7OTAYQ76002EXU@Macon2.Mercer.edu> Hi Guys, The final program for the 2008 Region III Symposium/Convention hosted by GSH is up on the website at www.histosearch.com/gsh in a .pdf and .doc format, as well as vendor registration forms. Print out you program and mail it in soon. The hotel reservation deadline is March 3rd, so make them soon. Thanks, Shirley Powell, GSH Secretary/Registrar From rjbuesa <@t> yahoo.com Tue Feb 12 14:41:32 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 12 14:41:36 2008 Subject: [Histonet] H & E stainers In-Reply-To: Message-ID: <477774.54198.qm@web61222.mail.yahoo.com> Get quotes (and ask for a demo) of the Sakura H&E stainer. Ren? J. "Hineline, Paula" wrote: Hello, We are researching histology H&E stainers for our lab. We have gotten quotes on the Leica ST 5020 multistainer and the Leica ST 4040 linear stainer. Recommendations would be appreciated. Thanks Paula Hineline, HT ASCP Pathology Laboratory phineline@grandpath.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From Erin.Martin <@t> ucsf.edu Tue Feb 12 15:23:51 2008 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Tue Feb 12 15:24:05 2008 Subject: [Histonet] Biopsy bags Message-ID: Hi everyone. Does anyone have an inexpensive source for the nylon biosy bags? The price quote we got from Thermo Shandon was about $2/bag - yikes!!! - for the small size. I hope that there is something cheaper out there... Thanks in advance! Erin Martin From HornHV <@t> archildrens.org Tue Feb 12 15:31:02 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Feb 12 15:31:54 2008 Subject: [Histonet] Biopsy bags In-Reply-To: References: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82B12@EMAIL.archildrens.org> Hi Erin, I found the old fashioned paper tea bags at Sunburst Bottle Company of Sacremento for only 5 cents each. The phone number is 916-929-3604. The cat# is tb 1 Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Erin Sent: Tuesday, February 12, 2008 3:24 PM To: histonet Subject: [Histonet] Biopsy bags Hi everyone. Does anyone have an inexpensive source for the nylon biosy bags? The price quote we got from Thermo Shandon was about $2/bag - yikes!!! - for the small size. I hope that there is something cheaper out there... Thanks in advance! Erin Martin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dermpathsy <@t> gmail.com Tue Feb 12 15:41:11 2008 From: dermpathsy <@t> gmail.com (Sate Hamza) Date: Tue Feb 12 15:41:29 2008 Subject: [Histonet] Coverslipping with the Dako Autostainer Message-ID: <8854ff80802121341i1204884ar376df31d12843ad4@mail.gmail.com> Colleagues, We use the DakoCytomation Autostainer and we are wondering what coverslipping instruments are being used to cover the slides with Dako slide labels .. Thank you .. Sate -- Sate Hamza, MD, FRCPC Dermatopathologist Winnipeg, Canada From mpence <@t> grhs.net Tue Feb 12 15:42:00 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Feb 12 15:42:07 2008 Subject: [Histonet] Biopsy bags In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3715@IS-E2K3.grhs.net> http://www.sunburstbottle.com/bags/tea-muslin -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Erin Sent: Tuesday, February 12, 2008 3:24 PM To: histonet Subject: [Histonet] Biopsy bags Hi everyone. Does anyone have an inexpensive source for the nylon biosy bags? The price quote we got from Thermo Shandon was about $2/bag - yikes!!! - for the small size. I hope that there is something cheaper out there... Thanks in advance! Erin Martin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From WWmn916 <@t> aol.com Tue Feb 12 22:42:24 2008 From: WWmn916 <@t> aol.com (WWmn916@aol.com) Date: Tue Feb 12 22:42:30 2008 Subject: [Histonet] little things in histology Message-ID: hello everyone two little detail questions 1) could soaking prefaced paraffin blocks a little too long in ammonia water effect the h+e staining of that slide causing a muddy h+e? 2)could leaving slides in 65-70 degree oven (about 30-45 minutes or longer) cause heat artifact and muddy the h+e stain? thanks deb king **************The year's hottest artists on the red carpet at the Grammy Awards. Go to AOL Music. (http://music.aol.com/grammys?NCID=aolcmp00300000002565) From ombadda3 <@t> gmail.com Wed Feb 13 06:17:55 2008 From: ombadda3 <@t> gmail.com (K M) Date: Wed Feb 13 06:18:01 2008 Subject: [Histonet] a reference for Streptavidin-biotin detecting system Message-ID: Dear Histonetters I am writting a paper in related to IHC. I will use streptavidin-biotin antigen detecting method. I have to put a reference for that method. Anyone tell me a title of a textbook , pages, publisher of that textbook in which this method mentioned. Thanks in advance Khalaf B. M.B.B.S Egypty From kmerriam2003 <@t> yahoo.com Wed Feb 13 06:39:39 2008 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Wed Feb 13 06:39:43 2008 Subject: [Histonet] Xylene Resistant Floors In-Reply-To: <16C83872A53F4346AA9C3A18E3A3AAB902276CE5@VHAV10MSGA1.v10.med.va.gov> Message-ID: <371446.12685.qm@web50305.mail.re2.yahoo.com> Hi Susan, The floors won't tear if you scrape them, the epoxy is a very hard polymer; it would be very difficult to damage them (and since the floors are "poured", they are seamless!). I come from the biotech/pharma industry and these types of floors are very common in animal rooms and laboratories in our industry. I have a friend that used to install them (he now works in Biotech!), nasty work, but the floors are great! Good luck. Kim "Weber, Susan (VHACLE)" wrote: Are you able to "scrape" paraffin off of them as well? Or does it tend to "gouge" when scrapping or moving equipment? I would be very interested in hearing as we have to present special concerns to the design committee this week. Thanks! Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Friday, February 08, 2008 9:36 AM To: kerry.l.crabb@gsk.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Xylene Resistant Floors Epoxy floors will do the trick, they are pretty expensive though. They need to be "poured" and allowed to cure for a certain amount of time before they can be used. They are resistant to just about anything. Kim kerry.l.crabb@gsk.com wrote: I've had a inquiry from another lab that is planning some remodeling. They're asking if there are any xylene resistant flooring that can be put down. Other can sealed concrete does anything exist? Kerry Crabb _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Kim Merriam, MA, HT(ASCP) Cambridge, MA --------------------------------- Never miss a thing. Make Yahoo your homepage. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Kim Merriam, MA, HT(ASCP) Cambridge, MA --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From rjbuesa <@t> yahoo.com Wed Feb 13 08:58:22 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 13 08:58:31 2008 Subject: [Histonet] Coverslipping with the Dako Autostainer In-Reply-To: <8854ff80802121341i1204884ar376df31d12843ad4@mail.gmail.com> Message-ID: <294454.91632.qm@web61225.mail.yahoo.com> I always used the film coverslipper by Sakura, perfectly compatible (size and material) with the labels. Ren? J. Sate Hamza wrote: Colleagues, We use the DakoCytomation Autostainer and we are wondering what coverslipping instruments are being used to cover the slides with Dako slide labels .. Thank you .. Sate -- Sate Hamza, MD, FRCPC Dermatopathologist Winnipeg, Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From lblazek <@t> digestivespecialists.com Wed Feb 13 09:02:11 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Feb 13 08:59:56 2008 Subject: [Histonet] H&E stainer Message-ID: <1F937FB30BDB7C4A9F39F83FEA8D379F9F521C@bruexchange1.digestivespecialists.com> Hello all, Does anyone have any experience good or bad with the Shandon Varistain Gemini? Thanks, Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com From rjbuesa <@t> yahoo.com Wed Feb 13 09:02:59 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 13 09:03:04 2008 Subject: [Histonet] little things in histology In-Reply-To: Message-ID: <466878.15435.qm@web61212.mail.yahoo.com> Amonia water has been described as affecting the H&E staining. Ammonia water on the faced off block is less helpful than a gentle soaking in arm water, or rubbing the surface with a gauze impregnated in mineral oil. Heating the slides don't UNLESS they are not completely drained (absolutely no water between the section and the slide) before heating them. Ren? J. WWmn916@aol.com wrote: hello everyone two little detail questions 1) could soaking prefaced paraffin blocks a little too long in ammonia water effect the h+e staining of that slide causing a muddy h+e? 2)could leaving slides in 65-70 degree oven (about 30-45 minutes or longer) cause heat artifact and muddy the h+e stain? thanks deb king **************The year's hottest artists on the red carpet at the Grammy Awards. Go to AOL Music. (http://music.aol.com/grammys?NCID=aolcmp00300000002565) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From rjbuesa <@t> yahoo.com Wed Feb 13 09:05:01 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 13 09:05:12 2008 Subject: [Histonet] a reference for Streptavidin-biotin detecting system In-Reply-To: Message-ID: <274288.72363.qm@web61221.mail.yahoo.com> Check the DAKO published "Handbook on Immunochemical staining methods" edited by Thomas Boenisch (2001) Ren? J. K M wrote: Dear Histonetters I am writting a paper in related to IHC. I will use streptavidin-biotin antigen detecting method. I have to put a reference for that method. Anyone tell me a title of a textbook , pages, publisher of that textbook in which this method mentioned. Thanks in advance Khalaf B. M.B.B.S Egypty _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From rjbuesa <@t> yahoo.com Wed Feb 13 09:07:40 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 13 09:07:50 2008 Subject: [Histonet] Xylene Resistant Floors In-Reply-To: <371446.12685.qm@web50305.mail.re2.yahoo.com> Message-ID: <893560.6425.qm@web61225.mail.yahoo.com> I would review your procedures protocol because no xylene on the floor should be an issue in any histology lab. It could be an accident, and you should not waste money in a more expensive floor, just because an accident may occur. Ren? J. Kim Merriam wrote: Hi Susan, The floors won't tear if you scrape them, the epoxy is a very hard polymer; it would be very difficult to damage them (and since the floors are "poured", they are seamless!). I come from the biotech/pharma industry and these types of floors are very common in animal rooms and laboratories in our industry. I have a friend that used to install them (he now works in Biotech!), nasty work, but the floors are great! Good luck. Kim "Weber, Susan (VHACLE)" wrote: Are you able to "scrape" paraffin off of them as well? Or does it tend to "gouge" when scrapping or moving equipment? I would be very interested in hearing as we have to present special concerns to the design committee this week. Thanks! Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Friday, February 08, 2008 9:36 AM To: kerry.l.crabb@gsk.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Xylene Resistant Floors Epoxy floors will do the trick, they are pretty expensive though. They need to be "poured" and allowed to cure for a certain amount of time before they can be used. They are resistant to just about anything. Kim kerry.l.crabb@gsk.com wrote: I've had a inquiry from another lab that is planning some remodeling. They're asking if there are any xylene resistant flooring that can be put down. Other can sealed concrete does anything exist? Kerry Crabb _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Kim Merriam, MA, HT(ASCP) Cambridge, MA --------------------------------- Never miss a thing. Make Yahoo your homepage. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Kim Merriam, MA, HT(ASCP) Cambridge, MA --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From Robinsoc <@t> mercyhealth.com Wed Feb 13 09:14:51 2008 From: Robinsoc <@t> mercyhealth.com (Cynthia Robinson) Date: Wed Feb 13 09:15:21 2008 Subject: [Histonet] H&E stainer In-Reply-To: <1F937FB30BDB7C4A9F39F83FEA8D379F9F521C@bruexchange1.digestivespecialists.com> References: <1F937FB30BDB7C4A9F39F83FEA8D379F9F521C@bruexchange1.digestivespecialists.com> Message-ID: <47B2B50B.59BC.00AF.0@mercyhealth.com> We have a Gemini and have used it for about 5 years. I like the stainer a lot, probably because it was our first automated stainer however, it was down from late July until November because we couldn't get parts. It tends to have issues with mold in the water drainage system. We pour chlorox down the water rinse areas once per week to control the problem. Also, the operating system is difficult to program onsite. I have had to do it on a Sat AM and it took me about 2 hours because the back up disc failed. If they have a new operating system and have resolved issues regarding replacement parts I would recommend it otherwise we have a Sakura linear stainer for paps that I also like. Cindi Robinson, HT(ASCP) Mercy Medical Center-Sioux City Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 712-279-2768 >>> "Blazek, Linda" 2/13/2008 9:02 AM >>> Hello all, Does anyone have any experience good or bad with the Shandon Varistain Gemini? Thanks, Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From minhan.tan <@t> gmail.com Wed Feb 13 09:21:54 2008 From: minhan.tan <@t> gmail.com (Min-Han Tan) Date: Wed Feb 13 09:22:03 2008 Subject: [Histonet] Double-staining with same secondary antibody? Message-ID: <920cc4a70802130721k3207c80aodf72a786fd6b1242@mail.gmail.com> Dear colleagues, I am planning a double stain of 2 antibodies - an endothelial marker and a smooth muscle antigen on human tissue, and have some questions about possibilities of using the same secondary antibody. Primary antibody for endothelial marker = goat species; tissue requires steam antigen retrieval. Primary antibody for smooth muscle antigen = mouse species; tissue does not require antigen retrieval. These bind to different areas of large blood vessels - one to endothelia, the other to smooth muscle. Currently, I only have immediate access to a biotinylated horse pan-specific marker (anti-goat, anti-mouse), and I have separately optimized the 2 markers using this same secondary antibody. Questions: 1. I wonder if it would be possible to pursue a double stain in a serial (not concurrent fashion), with DAB-Nickel for the first staining to "shelter", followed by NovaRed, using the same secondary antibody. 2. Is any elution step in between required, if I use the same secondary antibody for the 2nd sequential antibody? Shouldn't the 1st primary antibody be washed off, and in the worst case scenario, even if it isn't, will the 2nd red stain be obvious? :) Am pending purchase of other biotinylated secondaries. Or should I just purchase the DakoEnvision double staining kits? Chris van der Loos' posts at http://www.histosearch.com/histonet/Nov04A/HistonetRE.Elutionreagent.htmlwere very instructive. All advice would be appreciated! Thanks. Min-Han Tan From cmiller <@t> physlab.com Wed Feb 13 10:08:45 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Wed Feb 13 10:08:14 2008 Subject: [Histonet] In-service on Ht exam Message-ID: <001301c86e5a$b5f31d60$3d02a8c0@plab.local> Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 _____ From: Cheri Miller [mailto:cmiller@physlab.com] Sent: Wednesday, February 13, 2008 8:47 AM To: histonet-bounces@lists.utsouthwestern.edu Subject: In-service on Ht exam Is anyone aware of a prep class on taking the HT exam?? When I was studying for the exam there was a class given by a gentleman from CAP. The class I took was offered at the Tri State Histology Convention (IL, IA and Wis) It was held in Debuque, IA. It was so helpful and I have a few students that I would like to send to a similar class/in-service. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From lhunt <@t> uta.edu Wed Feb 13 10:50:08 2008 From: lhunt <@t> uta.edu (Laura Hunt) Date: Wed Feb 13 10:50:21 2008 Subject: [Histonet] Ethanol preserved tissues In-Reply-To: References: Message-ID: Hi Histo folks, So I have a friend you is interested in doing some histology on snake embryos. Unfortunately, the supply of this species of snake embryos is low and the only ones currently available have been museum specimens. Thus, they have been stored in ethanol for awhile. Are these hopeless or is there anyway to carry out some histology on them? Just wondering, Laura Hunt On Feb 12, 2008, at 12:00 PM, histonet- request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: prostate biopsy problem (Bell, Lynne) > 2. re/prostate quesitons (Joyce Cline) > 3. Re: re/prostate quesitons (Matt Bancroft) > 4. Tennessee Society for Histotechnology - Call for Vendors > (Hofecker, Jennifer L) > 5. re/prostate questions (Joyce Cline) > 6. $$$ Histology Position $$$ (April Sachau) > 7. Phoenix AZ histotechs (Pathrm35@comcast.net) > 8. RE: Histonet Digest, mouse pup euthanasia with CO2 (Jones, Lynne) > 9. new prostate problem! (Nick and Amanda) > 10. RELIA Histology Job Opportunity Alert 2/12/08 (Pam Barker) > 11. Formalin recycler (Sharon.Davis-Devine) > 12. RE: Formalin recycler (Laurie Colbert) > 13. Opening for a Core Lab Manager (Henry, Charlene) > 14. Re: new prostate problem! (pkromund@gundluth.org) > 15. Histology Core Lab Manager Position (Henry, Charlene) > 16. Histology Core Lab Manager Position (Henry, Charlene) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 11 Feb 2008 13:17:17 -0500 > From: "Bell, Lynne" > Subject: RE: [Histonet] prostate biopsy problem > To: "Joyce Cline" , > > Message-ID: > > We had the same problem a few years ago. Do you use sponges in your > cassette? We started using wet sponges instead of dry ones. We have > not had a problem since. > > Lynne Bell, HT (ASCP) > Central VT Hospital > P. O. Box 547 > Barre, VT 05641 > 802-371-4923 > > > > > > ------------------------------ > > Message: 2 > Date: Mon, 11 Feb 2008 13:52:16 -0500 > From: "Joyce Cline" > Subject: [Histonet] re/prostate quesitons > To: > Message-ID: <000701c86cdf$392aeb40$1d2a14ac@wchsys.org> > Content-Type: text/plain; charset="us-ascii" > > 1. We do not have the same problem with our breast core biopsies. > 2. We may have washing on block A and B but not on C,D or F and > washing on E, H, etc. > 3. The biopsies have no drying artifact on the edges. > 4. We use a low temp wax, Blue Ribbon paraffin for processing and > embedding. Our processor is set at 57. > 5. The tissue sections fall off during H&E staining. > 6. We do not use sponges, we use screened cassettes. > 7. The Stay on is used alone, not with the charged slides. > > Our pathologist that is our prostate specialist thinks each one of > us is > doing something different when we cut. We have all discussed this > matter > and can find no > differences when we cut. > > > ***** CONFIDENTIALITY NOTICE ***** > This message contains confidential information and is intended only > for > the individual named. If you are not the named addressee you should > not > disseminate, distribute or copy this e-mail. Please notify the sender > immediately by e-mail if you have received this e-mail by mistake and > delete this e-mail from your system. > > > > ------------------------------ > > Message: 3 > Date: Mon, 11 Feb 2008 11:17:43 -0800 (PST) > From: Matt Bancroft > Subject: Re: [Histonet] re/prostate quesitons > To: Joyce Cline , histonet@lists.utsouthwestern.edu > Message-ID: <446182.81960.qm@web63409.mail.re1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > The issue that I have found is even when one uses + charged slides, > you still need to make sure that all the water is gone from under > the tissue section. When I cut needle biopsies (kidney, prostate, > or breast) after I get the section on the slide I take my forceps > tare a little slit in the corner of my sections and use a kim wipe > to absorb the water. I have not had a problem with sections falling > off since I started doing this. I also use HISTOBOND slides from > StatLab medical products. These slides are very sticky (sometimes to > much). > > Matt Bancroft HT(ASCP) > Laboratory Manager > > > Joyce Cline wrote: > 1. We do not have the same problem with our breast core biopsies. > 2. We may have washing on block A and B but not on C,D or F and > washing on E, H, etc. > 3. The biopsies have no drying artifact on the edges. > 4. We use a low temp wax, Blue Ribbon paraffin for processing and > embedding. Our processor is set at 57. > 5. The tissue sections fall off during H&E staining. > 6. We do not use sponges, we use screened cassettes. > 7. The Stay on is used alone, not with the charged slides. > > Our pathologist that is our prostate specialist thinks each one of > us is > doing something different when we cut. We have all discussed this > matter > and can find no > differences when we cut. > > > ***** CONFIDENTIALITY NOTICE ***** > This message contains confidential information and is intended only > for > the individual named. If you are not the named addressee you should > not > disseminate, distribute or copy this e-mail. Please notify the sender > immediately by e-mail if you have received this e-mail by mistake and > delete this e-mail from your system. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Looking for last minute shopping deals? Find them fast with Yahoo! > Search. > > ------------------------------ > > Message: 4 > Date: Mon, 11 Feb 2008 13:25:00 -0600 > From: "Hofecker, Jennifer L" > Subject: [Histonet] Tennessee Society for Histotechnology - Call for > Vendors > To: "histonet" > Message-ID: > <898D946569A27444B65667A49C0740520156449C@mailbe06.mc.vanderbilt.edu> > Content-Type: text/plain; charset="us-ascii" > > Happy Monday to all. I just wanted to take a moment and post a call > for > vendors. The TSH meeting is June 19-21 in Townsend, TN. Townsend is > located in the scenic Smoky Mountain area. The meeting will be held at > the valley view lodge. If you are interested in exhibiting at our > meeting, please contact me for complete information. The deadline to > have your company listed in the program as a sponsor is March 1. > > The official programs should be mailed by April 1 and I will post > contact info again at that time for anyone interested in attending the > meeting who may not receive a program. > > Thanks and have a great week! > > > Jennifer L. Hofecker, HT(ASCP) > Vanderbilt University Medical Center > Division of Neuropathology > Nashville, TN > ph. (615)343-0083 > fax. (615)343-7089 > NSH Quality Control Committee Chair > Tennessee Society for Histotechnology Secretary > 2008 TSH Meeting Exhibit Coordinator > > > > > ------------------------------ > > Message: 5 > Date: Mon, 11 Feb 2008 14:31:41 -0500 > From: "Joyce Cline" > Subject: [Histonet] re/prostate questions > To: > Message-ID: <000701c86ce4$ba8c4f80$1d2a14ac@wchsys.org> > Content-Type: text/plain; charset="us-ascii" > > Our slides are dried vertically on plastic slide racks. I do not use > Xylene, I use Formula 83. > > > ***** CONFIDENTIALITY NOTICE ***** > This message contains confidential information and is intended only > for > the individual named. If you are not the named addressee you should > not > disseminate, distribute or copy this e-mail. Please notify the sender > immediately by e-mail if you have received this e-mail by mistake and > delete this e-mail from your system. > > > > ------------------------------ > > Message: 6 > Date: Mon, 11 Feb 2008 14:06:16 -0600 > From: "April Sachau" > Subject: [Histonet] $$$ Histology Position $$$ > To: "Hofecker, Jennifer L" , > "histonet" > Message-ID: > > <7E3ACD48BA6E26408F3188FBF08693F7010D4C56@titansbs1.corp.titanmed.com> > Content-Type: text/plain; charset="us-ascii" > > Hello Histonetters! > > I have an awesome position available in the Midwest. Great > compensation > package!!! It is a dayshift position for a minimum of 13 weeks. > Possible permanent placement! Please let me know if you are > interested > or would like additional information! > > > April Sachau > Titan Medical Group > Staff Supervisor > Phone (866) 332-9600 Ext. 1023 > Fax (402) 332-5181 > asachau@titanmed.com > > see us on the web at www.titanmed.com > > > > ------------------------------ > > Message: 7 > Date: Mon, 11 Feb 2008 22:31:07 +0000 > From: Pathrm35@comcast.net > Subject: [Histonet] Phoenix AZ histotechs > To: histonet@lists.utsouthwestern.edu > Message-ID: > <021120082231.3815.47B0CCAA000F06C100000EE72215551724CACC039D089B0EAF@comcast.net > > > > Content-Type: text/plain > > Could a histotech from the Phoenix, AZ area please contact me? > Thanks, > Ron Martin > > ------------------------------ > > Message: 8 > Date: Mon, 11 Feb 2008 17:03:12 -0600 > From: "Jones, Lynne" > Subject: [Histonet] RE: Histonet Digest, mouse pup euthanasia with CO2 > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > > > > Content-Type: text/plain; charset="us-ascii" > > Hello to the list - > I have little histology experience, but I've dealt with > complications arising from both anesthesia and euthanasia in lab > animals. Standard protocols don't work for every model. > > Neonatal rodents are very resistant to hypoxia (death can take up to > 50 minutes, depending on age and strain). Our institutional policy > is that neonatal rats and mice must remain inside the CO2 chamber > for a minimum of 20 minutes after the gas flow is turned off if CO2 > is the sole agent used for euthanasia. Many Institutional Animal > Care and Use Committees suggest consideration of alternative methods > for neonates, or that CO2 anesthesia be followed by a physical method. > > FWIW, the paper below describes some of the biological effects > associated with different euthanasia techniques. > http://www.aclam.org/print/report_rodent_euth.pdf > > Based on the description of the brains, my suspicion is that the > pups sat too long post-mortem before being fixed, or (going way out > on a limb) that acidosis might have created problems if the formalin > wasn't buffered. > > Lynne Jones > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu > ] On Behalf Of histonet-request@lists.utsouthwestern.edu > Sent: Monday, February 11, 2008 12:07 PM > To: histonet@lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 51, Issue 17 > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Fwd: [Histonet] Re: Mouse pups and processing (Greg Dobbin) > 2. Verhoeff/ Trichome procedure (karenadams@comcast.net) > 3. RE: Verhoeff/ Trichome procedure (Fail, Mildred M.) > 4. Iron staining (Till, Renee) > 5. RE: OT: the weather (Ingles Claire) > 6. prostate biopsy problem (Joyce Cline) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 11 Feb 2008 08:54:11 -0400 > From: "Greg Dobbin" > Subject: Fwd: [Histonet] Re: Mouse pups and processing > To: > Message-ID: > Content-Type: text/plain; charset=US-ASCII > > > > Greg Dobbin, R.T. > Chief Technologist, Histology Lab > Dept. of Laboratory Medicine, > Queen Elizabeth Hospital, > P.O. Box 6600 > Charlottetown, PE C1A 8T5 > Phone: (902) 894-2337 > Fax: (902) 894-2385 > > There is some merit in doing the right thing rather badly, > but absolutely none in doing the wrong thing excellently! > >>>> Greg Dobbin 2/11/2008 8:53 AM >>> > Hi Pam, > I participated on a project about 10 years ago where the rat pups were > anesthetized using an overdose of halothane followed by exanguinationn > (carotid and/or renal arteries). Here are the 2 relevant references: > Comp Med. 2001 Apr;51(2):134-7. > Histol Histopathol. 2002 Oct;17(4):1067-76. > Good luck. > Greg > > > Greg Dobbin, R.T. > Chief Technologist, Histology Lab > Dept. of Laboratory Medicine, > Queen Elizabeth Hospital, > P.O. Box 6600 > Charlottetown, PE C1A 8T5 > Phone: (902) 894-2337 > Fax: (902) 894-2385 > > There is some merit in doing the right thing rather badly, > but absolutely none in doing the wrong thing excellently! > >>>> 2/10/2008 9:57 AM >>> > Thank you Amos and I can appreciate your problem. Your discription of > sqishy > is > prefect. I have also referred to the outer skin as lacy in appearance > when > attempting to section it. The other point I would make is the brain > is > mush > with no form as if it is autolysized totally. > > I have tried splitting the abdomen and it does not help or is so > minimal it > doesn't help much. I have tried changing the processor and always > process > other tissues at the same time to show the PI it is not the reagents > and > paraffin. We have processed other pups before this over the last year > and know > they were not killed with CO2. They work great and section great (I > still > split > the abdomen). > > I am at a loss and finally turned to all of you on HistoNet help. You > are > great > and even though we may not have the answer yet we will I am sure of > it. > I know > this is an ongoing problem for researchers with pups. Maybe with this > CO2 > thing being a popular way for sacrificing animals we have hit on > something that > happens in pups and not adults. I had not really ever worried about > it > until > now as I did my own sacrifice and it was fine. I wish they weren't so > small I > would attempt prefusion with a 2cc syringe and blunt needle as a last > resort. > > > I want to THANK everyone who has and will take the time to answer and > help me > with this issue. At least I can show the PI our lab is not the only > one with > the problem and we are all attempting to find a way out of it. I > think > from > all I have seen we should be more aware of how the pups are sacrificed > and note > any differences between the way our PI's sacrifice them. Maybe all of > the > material submitted to the Histology area for research on pups and > other > animals > should be recorded and we might see differences they don't in method. > Recommendations from IACOCC do not take our area of histology into > consideration as they are controlling the most humane and painless > method of > sacrificing animals over all. Perhaps one of us can get a paper out > of > this or > a talk in the future that helps everyone. > > Who knows I even checked the phase of the moon this last time and it > did not > help. > > Thanks again and still looking. If I solve this you will all be teh > first to > know. > > Pam > > Quoting Amos Brooks : > >> Pam, >> We were having a similar problem with one of our researcher's P0 > mice. >> I don't know how they sacrificed them, but they too dod not process > at all. >> They were squishy! >> I reccommended they either bisect or at least make a good > midline >> incision prior to fixation. It still isn't perfect (is it ever?), but > it >> seems to be a bit better. This allows better solution penetration. > Give it a >> whirl and let us know how it goes. >> >> Amos >> >> Message: 13 >> Date: Fri, 08 Feb 2008 15:52:34 -0500 >> From: Pamela Marcum >> Subject: [Histonet] Mouse pups and processing >> To: Histonet@lists.utsouthwestern.edu >> Message-ID: <6.2.5.6.2.20080208154217.01c8a9b8@vet.upenn.edu> >> Content-Type: text/plain; charset="us-ascii"; format=flowed >> >> >> >> I have been struggling with this for a while and need some help. We >> currently have a project with 0 day mouse pups that are allowed to > be >> born normally and then sacrificed with CO2. We had several groups >> earlier that were sacrificed a different way and they processed and >> sectioned beautifully. >> >> These don't seem to fix well, dehydrate, clear or infiltrate worth a >> darn. Since this is my first time using CO2 for sacrifice I need to >> find out if it causes a problem or if I am just losing it. I have >> not ever had this problem before and even re-processing does not >> help. I know they are not infiltrating as they are floating in >> paraffin at the end if I remove them from the processor to a vat of >> paraffin. They will not sink. The pups are 1.2 to 1.3 grams each. >> >> If you can suggest a better way to sacrifice them please let me >> know. Killing the mother and perfusing her is not an option as > these >> are not our mice. They are being given as favor so I am limited to >> some extent. >> >> This was an overnight process with slightly altered alcohols to 45 >> minutes each, 1 xylene at 30 minutes and 2 Shandon Xylene Substitues >> at 10 hour each to 4 paraffins at 45 X2 and 1 hour X2. >> >> Best Regards, >> >> Pamela A Marcum >> Manager, Histology Special Procedures >> University of Pennsylvania >> School of Veterinary Medicine >> R.S. Reynolds Jr. CORL >> New Bolton Center >> 382 West Street Road >> Kennett Square, PA 19348 >> >> Phone - 610-925-6278 >> Fax - 610-925-8120 >> E-mail - pmarcum@vet.upenn.edu > > > The materials in this message are private and may contain Protected > Healthcare Information. If you are not the intended recipient, be > advised that any unauthorized use, disclosure, copying or the taking > of any action in reliance on the contents of this information is > strictly prohibited. If you have received this email in error, > please immediately notify the sender via telephone or return mail. > > > > ------------------------------ > > Message: 9 > Date: Tue, 12 Feb 2008 20:30:24 +1300 > From: Nick and Amanda > Subject: [Histonet] new prostate problem! > To: histonet@lists.utsouthwestern.edu > Message-ID: <000d01c86d49$21db4ec0$c8084979@bow1> > Content-Type: text/plain; charset="iso-8859-1" > > Hi! > > We have recently come up against a staining issue where our prostate > cores have patches which are not staining. Our Pathologist tells us > this is common in a particular High grade diagnosis so this is a > problem when it is happening in all prostate cases.. It does not > seem to be affecting other tissue staining (perhaps the odd one! no > examples could be provided!! Could it possibly be drying out of the > cores prior to being placed in formalin?? > > Any Ideas welcome > > Just not sure! > Wellington, NZ > > ------------------------------ > > Message: 10 > Date: Tue, 12 Feb 2008 08:14:57 -0500 > From: "Pam Barker" > Subject: [Histonet] RELIA Histology Job Opportunity Alert 2/12/08 > To: "'Histonet'" > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Hi Histonetters!! > Just wanted to do a quick post on some of the jobs I am currently > working on. These clients are all looking for ASCP certified or > eligible people interested in full time dayshift permanent positions. > They offer excellent compensation, benefits and relocation > assistance if > needed. > If you or someone you know is interested please contact me and please > feel free to pass this information along to others as well > Here is a list of the locations where I have some great opportunities: > Texas - Multiple locations management and tech positions > South Carolina - tech position > Washington - management and tech positions > Montana - tech position > Pennsylvania - multiple locations tech positions > New Hampshire - tech position > California - tech positions - ASCP certification is required > Virginia - tech position > > Remember it never hurts to look. You can reach me at 866-60-RELIA > (866-607-3542) or at relia1@earthlink.net > > Have a Happy Valentines Day!!! > > Thanks-Pam > > > Thank You! > > > Pam Barker > President > RELIA > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > Toll Free(866)-607-3542 > Fax: (407)678-2788 > E-mail: relia1@earthlink.net > > www.myspace.com/pamatrelia > > > > ------------------------------ > > Message: 11 > Date: Tue, 12 Feb 2008 08:11:49 -0600 > From: "Sharon.Davis-Devine" > Subject: [Histonet] Formalin recycler > To: > Message-ID: > <44780C571F28624DBB446DE55C4D733A021E08BE@EXCHANGEBE1.carle.com> > Content-Type: text/plain; charset="us-ascii" > > Presently we have two CBG recycling machines; one recycles alcohol and > the other formalin. We seem to always be backed up on formalin > because > it takes so long to run a cycle. Is there some other system out there > that offers a faster turn around time? Any suggestions would be > greatly > appreciated. Thanks. > > > > Sharon Davis-Devine, CT (ASCP) > > Cytology Supervisor > > Carle Clinic > > 602 West University > > Urbana, Illinois 61801 > > Phone: 217-383-3572 > > Email: sharon.davis-devine@carle.com > > > > > > ------------------------------ > > Message: 12 > Date: Tue, 12 Feb 2008 07:27:26 -0800 > From: "Laurie Colbert" > Subject: RE: [Histonet] Formalin recycler > To: "Sharon.Davis-Devine" , > > Message-ID: > <57BE698966D5C54EAE8612E8941D76830267DD39@EXCHANGE3.huntingtonhospital.com > > > > Content-Type: text/plain; charset="us-ascii" > > Creative Waste Solutions has a formalin recycler that is very fast and > easy to use. We love it! We use our CBG recycler for alcohol and > xylene and the Creative Waste recycler for the formalin. They can be > reached at (888) 795-8300. > > Laurie Colbert > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Sharon.Davis-Devine > Sent: Tuesday, February 12, 2008 6:12 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Formalin recycler > > Presently we have two CBG recycling machines; one recycles alcohol and > the other formalin. We seem to always be backed up on formalin > because > it takes so long to run a cycle. Is there some other system out there > that offers a faster turn around time? Any suggestions would be > greatly > appreciated. Thanks. > > > > Sharon Davis-Devine, CT (ASCP) > > Cytology Supervisor > > Carle Clinic > > 602 West University > > Urbana, Illinois 61801 > > Phone: 217-383-3572 > > Email: sharon.davis-devine@carle.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 13 > Date: Tue, 12 Feb 2008 09:59:45 -0600 > From: "Henry, Charlene" > Subject: [Histonet] Opening for a Core Lab Manager > To: "Histonet@lists.utsouthwestern.edu" > > Message-ID: > <03E1F5968F60C5448635D49D38B283ED03F507BF@SJMEMXMBS11.stjude.sjcrh.local > > > > Content-Type: text/plain; charset="us-ascii" > > We currently have an opening for a Histology Core Lab Manager in our > new Histology Core Lab that is due to be completed within the next > couple of months. If you are interested, you can send me an email or > visit www.stjue.org. I'll have to say that St > Jude is one of the greatest places I have ever worked and would > recommend this position to anyone looking for a research lab to > manage. > Thanks, > > Charlene Henry HT (ASCP), QIHC > Anatomic Pathology Section Head > Department of Pathology > St. Jude Children's Research Hospital > 901-495-3191 > fax 901-495-3100 > > > > ------------------------------ > > Message: 14 > Date: Tue, 12 Feb 2008 10:29:55 -0600 > From: pkromund@gundluth.org > Subject: Re: [Histonet] new prostate problem! > To: Nick and Amanda > Cc: histonet@lists.utsouthwestern.edu, > histonet-bounces@lists.utsouthwestern.edu > Message-ID: > > > > Content-Type: text/plain; charset=US-ASCII > > Yes, drying prior to being placed in formalin does have adverse > affects on > staining. > > > > Nick and Amanda > > adise.net.nz> To > Sent by: > histonet@lists.utsouthwestern.edu > histonet- > bounces@ cc > lists.utsouthwest > ern.edu > Subject > [Histonet] new prostate problem! > > 02/12/2008 01:30 > AM > > > > > > > > Hi! > > We have recently come up against a staining issue where our prostate > cores > have patches which are not staining. Our Pathologist tells us this is > common in a particular High grade diagnosis so this is a problem > when it is > happening in all prostate cases.. It does not seem to be affecting > other > tissue staining (perhaps the odd one! no examples could be > provided!! Could > it possibly be drying out of the cores prior to being placed in > formalin?? > > Any Ideas welcome > > Just not sure! > Wellington, NZ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 15 > Date: Tue, 12 Feb 2008 10:48:00 -0600 > From: "Henry, Charlene" > Subject: [Histonet] Histology Core Lab Manager Position > To: "Histonet@lists.utsouthwestern.edu" > > Message-ID: > <03E1F5968F60C5448635D49D38B283ED03F507C0@SJMEMXMBS11.stjude.sjcrh.local > > > > Content-Type: text/plain; charset="us-ascii" > > In my earlier email, I left out part of the email address and it is > not even Monday. The correct link is www.stjude.org >; sorry for the confusion. > Thanks, > > Charlene Henry HT (ASCP), QIHC > Anatomic Pathology Section Head > Department of Pathology > St. Jude Children's Research Hospital > 901-495-3191 > fax 901-495-3100 > > > > ------------------------------ > > Message: 16 > Date: Tue, 12 Feb 2008 10:59:16 -0600 > From: "Henry, Charlene" > Subject: [Histonet] Histology Core Lab Manager Position > To: "Histonet@lists.utsouthwestern.edu" > > Message-ID: > <03E1F5968F60C5448635D49D38B283ED03F507C1@SJMEMXMBS11.stjude.sjcrh.local > > > > Content-Type: text/plain; charset="us-ascii" > > If interested in this position go to www.stjude.org > and under "Jobs" the position # is 17078 and the title of this > position is "Laboratory Manager Veterinary Histology Core". > Thanks, > > Charlene Henry HT (ASCP), QIHC > Anatomic Pathology Section Head > Department of Pathology > St. Jude Children's Research Hospital > 901-495-3191 > fax 901-495-3100 > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 51, Issue 18 > **************************************** Laura R Hunt, PhD Post-doctoral associate University of Texas at Arlington ph: 817-272-1499 fax: 817-272-2855 lhunt@uta.edu From melissa.mazan <@t> tufts.edu Wed Feb 13 11:03:50 2008 From: melissa.mazan <@t> tufts.edu (Melissa Mazan) Date: Wed Feb 13 11:04:00 2008 Subject: [Histonet] vimentin In-Reply-To: <200802131658.m1DGwEYo013962@mail-proofpoint-6a.usg.tufts.edu> References: <200802131658.m1DGwEYo013962@mail-proofpoint-6a.usg.tufts.edu> Message-ID: <47B322F6.4000703@tufts.edu> We've used bd biosciences mouse monoclonal 550513 - 1:100 on FFPE lung tissue overnight at 4C with Alexafluor secondary for 30 min at 37C. We've also used the abcam chicken vimentin - works pretty well but huge background on westerns unless you use nitrocellulose. Melissa histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Used equipment (Renko, Heather D.) > 2. ? vimentin IHC ab from ABR bioreagents (Kathy Cormier) > 3. H & E stainers (Hineline, Paula) > 4. Region III Meeting Program (Shirley Powell) > 5. Re: H & E stainers (Rene J Buesa) > 6. Biopsy bags (Martin, Erin) > 7. RE: Biopsy bags (Horn, Hazel V) > 8. Coverslipping with the Dako Autostainer (Sate Hamza) > 9. RE: Biopsy bags (Mike Pence) > 10. little things in histology (WWmn916@aol.com) > 11. a reference for Streptavidin-biotin detecting system (K M) > 12. RE: Xylene Resistant Floors (Kim Merriam) > 13. Re: Coverslipping with the Dako Autostainer (Rene J Buesa) > 14. H&E stainer (Blazek, Linda) > 15. Re: little things in histology (Rene J Buesa) > 16. Re: a reference for Streptavidin-biotin detecting system > (Rene J Buesa) > 17. RE: Xylene Resistant Floors (Rene J Buesa) > 18. Re: H&E stainer (Cynthia Robinson) > 19. Double-staining with same secondary antibody? (Min-Han Tan) > 20. In-service on Ht exam (Cheri Miller) > 21. Ethanol preserved tissues (Laura Hunt) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 12 Feb 2008 12:04:20 -0600 > From: "Renko, Heather D." > Subject: [Histonet] Re: Used equipment > To: histonet@lists.utsouthwestern.edu > Message-ID: > <40026EDDE64CDA47AB382C52619ACD3C0A09DD2B@pmc-rfd-mx01.intranet.osfnet.org> > > Content-Type: text/plain; charset=iso-8859-1 > > I have a Microm 760X H&E stainer that we are replacing. If you know of anyone that might want it or even for parts email me directly. > > Heather D. Renko, Histology Coordinator > OSF Saint Anthony Medical Center > 5666 East State Street > Rockford, Illinois 61108 > Direct: 815-395-5410 > Heather.D.Renko@osfhealthcare.org > > > ============================================================================== > The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. > ============================================================================== > > > ------------------------------ > > Message: 2 > Date: Tue, 12 Feb 2008 14:21:41 -0500 > From: "Kathy Cormier" > Subject: [Histonet] ? vimentin IHC ab from ABR bioreagents > To: > Message-ID: <000a01c86dac$7f5fb3c0$92003712@mit.edu> > Content-Type: text/plain; charset="iso-8859-1" > > Hi All, > > Has anyone tried the vimetin mouse mono ab from ABR bioreagents? I need to find a vimentin that will work on FFPE mouse tissue, and I do not want to spend $300 if the ab does not work well.... Any other ab that anyone knows works well? (yes, I checked the archives!) > > Thanks! > > Kathy > DCM MIT > > ------------------------------ > > Message: 3 > Date: Tue, 12 Feb 2008 14:02:29 -0600 > From: "Hineline, Paula" > Subject: [Histonet] H & E stainers > To: > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > > Hello, > We are researching histology H&E stainers for our lab. We have gotten > quotes on the Leica ST 5020 multistainer and the Leica ST 4040 linear > stainer. Recommendations would be appreciated. > Thanks > Paula Hineline, HT ASCP > Pathology Laboratory > phineline@grandpath.com > > > > > ------------------------------ > > Message: 4 > Date: Tue, 12 Feb 2008 15:17:13 -0500 > From: Shirley Powell > Subject: [Histonet] Region III Meeting Program > To: histonet@lists.utsouthwestern.edu > Message-ID: <01MR7OTAYQ76002EXU@Macon2.Mercer.edu> > Content-Type: text/plain; charset=us-ascii > > Hi Guys, > > The final program for the 2008 Region III Symposium/Convention hosted by GSH > is up on the website at www.histosearch.com/gsh in a .pdf and .doc format, > as well as vendor registration forms. Print out you program and mail it in > soon. The hotel reservation deadline is March 3rd, so make them soon. > > Thanks, > Shirley Powell, GSH Secretary/Registrar > > > > > ------------------------------ > > Message: 5 > Date: Tue, 12 Feb 2008 12:41:32 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] H & E stainers > To: "Hineline, Paula" , > histonet@lists.utsouthwestern.edu > Message-ID: <477774.54198.qm@web61222.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Get quotes (and ask for a demo) of the Sakura H&E stainer. > Ren? J. > > "Hineline, Paula" wrote: > > Hello, > We are researching histology H&E stainers for our lab. We have gotten > quotes on the Leica ST 5020 multistainer and the Leica ST 4040 linear > stainer. Recommendations would be appreciated. > Thanks > Paula Hineline, HT ASCP > Pathology Laboratory > phineline@grandpath.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Looking for last minute shopping deals? Find them fast with Yahoo! Search. > > ------------------------------ > > Message: 6 > Date: Tue, 12 Feb 2008 13:23:51 -0800 > From: "Martin, Erin" > Subject: [Histonet] Biopsy bags > To: "histonet" > Message-ID: > > Content-Type: text/plain; charset=iso-8859-1 > > Hi everyone. > > Does anyone have an inexpensive source for the nylon biosy bags? The price quote we got from Thermo Shandon was about $2/bag - yikes!!! - for the small size. I hope that there is something cheaper out there... > > Thanks in advance! > > Erin Martin > > > > > ------------------------------ > > Message: 7 > Date: Tue, 12 Feb 2008 15:31:02 -0600 > From: "Horn, Hazel V" > Subject: RE: [Histonet] Biopsy bags > To: "Martin, Erin" , "histonet" > > Message-ID: > <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82B12@EMAIL.archildrens.org> > Content-Type: text/plain; charset="us-ascii" > > Hi Erin, > I found the old fashioned paper tea bags at Sunburst Bottle Company of > Sacremento for only 5 cents each. The phone number is 916-929-3604. > The cat# is tb 1 > > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Histology > Arkansas Children's Hospital > 800 Marshall Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax 501.364.3912 > > visit us on the web at: www.archildrens.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, > Erin > Sent: Tuesday, February 12, 2008 3:24 PM > To: histonet > Subject: [Histonet] Biopsy bags > > Hi everyone. > > Does anyone have an inexpensive source for the nylon biosy bags? The > price quote we got from Thermo Shandon was about $2/bag - yikes!!! - for > the small size. I hope that there is something cheaper out there... > > Thanks in advance! > > Erin Martin > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 8 > Date: Tue, 12 Feb 2008 15:41:11 -0600 > From: "Sate Hamza" > Subject: [Histonet] Coverslipping with the Dako Autostainer > To: histonet@lists.utsouthwestern.edu > Message-ID: > <8854ff80802121341i1204884ar376df31d12843ad4@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Colleagues, > > We use the DakoCytomation Autostainer and we are wondering what > coverslipping instruments are being used to cover the slides with Dako slide > labels .. > > Thank you .. > > Sate > > -- Melissa R. Mazan, DVM, Diplomate ACVIM Associate Professor and Director of Sports Medicine Tufts Cummings School of Veterinary Medicine 200 Westboro Road North Grafton, MA 01536 508-887-4589 From ian.montgomery <@t> bio.gla.ac.uk Wed Feb 13 11:05:45 2008 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Wed Feb 13 11:05:54 2008 Subject: [Histonet] (no subject) Message-ID: <005301c86e62$ac685c30$6424d182@IBLS.GLA.AC.UK> When the select few are sitting in the nuclear bunkers the dominant species on the planet will be nematodes. I've just started a project for my Zoologist colleagues studying these wee devils and I'm convinced they are the mega-organism. Formaldehyde, not a problem, couple of weeks later, give them a rinse and away they swim. Osmium tetroxide, "are they trying to annoy me with this slightly noxious compound." Managing to fix them is hard enough but processing for sectioning, a nightmare. Does anyone have experience processing these beasts? Hints and tips would be very welcome. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, G12 8QQ. From Ronald.Houston <@t> nationwidechildrens.org Wed Feb 13 11:21:01 2008 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Wed Feb 13 11:21:31 2008 Subject: [Histonet] (no subject) In-Reply-To: <005301c86e62$ac685c30$6424d182@IBLS.GLA.AC.UK> Message-ID: <979FF5962E234F45B06CF0DB7C1AABB21546ED5F@chi2k3ms01.columbuschildrens.net> Ian, Try this http://microscopy.tamu.edu/lab-protocols/light-microscopy-protocols.html Or maybe a good malt would work? On second thoughts, you'd probably be better keeping the malt for yourself. Good luck, aw ra' best and Slainthe Ronnie Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 Ronald.Houston@NationwideChildrens.org Columbus Children's Hospital is now Nationwide Children's Hospital www.NationwideChildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian Montgomery Sent: Wednesday, February 13, 2008 12:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) When the select few are sitting in the nuclear bunkers the dominant species on the planet will be nematodes. I've just started a project for my Zoologist colleagues studying these wee devils and I'm convinced they are the mega-organism. Formaldehyde, not a problem, couple of weeks later, give them a rinse and away they swim. Osmium tetroxide, "are they trying to annoy me with this slightly noxious compound." Managing to fix them is hard enough but processing for sectioning, a nightmare. Does anyone have experience processing these beasts? Hints and tips would be very welcome. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, G12 8QQ. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From oshel1pe <@t> cmich.edu Wed Feb 13 11:32:43 2008 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Wed Feb 13 11:33:17 2008 Subject: [Histonet] (no subject) In-Reply-To: <005301c86e62$ac685c30$6424d182@IBLS.GLA.AC.UK> References: <005301c86e62$ac685c30$6424d182@IBLS.GLA.AC.UK> Message-ID: Ian, A couple of useful web sites: http://www.wormatlas.org/ and Dave Hall's lab at Albert Einstein Coll. of Medicine: http://wormem.aecom.yu.edu/ Jay Campbell at U Wisconsin has done a bunch of C. elegans EM. jmcampbe@wisc.edu Mind, most of the people I know are either doing EM or confocal on whole-mount or squish-mount worms. Not light microscopic sections, so I'm not sure how useful these contacts will be. The big trick for fixation & processing at the EM level is high-pressure freezing followed by freeze-substitution for embedding. For "regular" light histology & sectioning, I'd suggest lopping off the head & tail & maybe bisecting the body, then embed in JB-4 or similar resin and cut with a glass knife. Paraffin embedding and steel knives won't cut it. Phil > When the select few are sitting in the nuclear bunkers the >dominant species on the planet will be nematodes. I've just started a >project for my Zoologist colleagues studying these wee devils and I'm >convinced they are the mega-organism. Formaldehyde, not a problem, couple of >weeks later, give them a rinse and away they swim. Osmium tetroxide, "are >they trying to annoy me with this slightly noxious compound." Managing to >fix them is hard enough but processing for sectioning, a nightmare. Does >anyone have experience processing these beasts? Hints and tips would be very >welcome. > >Ian. > >Dr. Ian Montgomery, >Histotechnology, >I.B.L.S. Support Unit, >Thomson Building, >University of Glasgow, >G12 8QQ. -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576 From Dorothy.L.Webb <@t> HealthPartners.Com Wed Feb 13 11:41:28 2008 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Wed Feb 13 11:41:35 2008 Subject: [Histonet] Posting position Message-ID: <0E394B648E5284478A6CCB78E5AFDA270563548B@hpes1.HealthPartners.int> Regions Hospital Department of Pathology - Laboratory Information Services has an exciting new open position, please see the attached document to learn more! Inquires can be addressed to www.regionshospital.com under career opportunities. <> Dorothy Webb, HT (ASCP) Histology Technical Supervisor Regions Hospital, Pathology Department 640 Jackson Street, Saint Paul, MN 55101-2595 Phone: 651-254-2962 Fax: 651-254-2741 Regions Hospital is part of the HealthPartners family of care ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From lesley.bechtold <@t> jax.org Wed Feb 13 11:43:52 2008 From: lesley.bechtold <@t> jax.org (Lesley Bechtold) Date: Wed Feb 13 11:44:58 2008 Subject: [Histonet] Histology Benchmarking Survey Message-ID: <20080213124352372.00000002468@spikey> To the Histology Listserver Users, Scientific Services at The Jackson Laboratory is conducting benchmarking surveys in an effort to develop an understanding of the best current practices offered by peer institutions. The goal is to gather quality data that can be used to assist us and all survey participants with operational assessment and planning. Survey participants will receive compiled results which will be de-identified before distribution. The link is below and must be completed by February 29th. Thank you in advance for your participation! Histology http://www.surveymonkey.com/s.aspx?sm=Tw8280iqIdlQCGyRRLkhyw_3d_3d Lesley S. Bechtold Senior Manager, Histopathology & Microscopy Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 ________________________________ From pmarcum <@t> vet.upenn.edu Wed Feb 13 12:00:28 2008 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Wed Feb 13 12:01:02 2008 Subject: [Histonet] (no subject) In-Reply-To: <005301c86e62$ac685c30$6424d182@IBLS.GLA.AC.UK> References: <005301c86e62$ac685c30$6424d182@IBLS.GLA.AC.UK> Message-ID: <6.2.5.6.2.20080213125738.01c80188@vet.upenn.edu> Here Ithought it would be the cock roach or one of the subspecies of it. I thought osmium tetroxide would kill anything. My technician tells me the only way they get rid of them in animals that had become infected was to worm them. I guess the worm medication was the answer. Good Luck. Pam Marcum At 12:05 PM 2/13/2008, Ian Montgomery wrote: > When the select few are sitting in the nuclear bunkers the >dominant species on the planet will be nematodes. I've just started a >project for my Zoologist colleagues studying these wee devils and I'm >convinced they are the mega-organism. Formaldehyde, not a problem, couple of >weeks later, give them a rinse and away they swim. Osmium tetroxide, "are >they trying to annoy me with this slightly noxious compound." Managing to >fix them is hard enough but processing for sectioning, a nightmare. Does >anyone have experience processing these beasts? Hints and tips would be very >welcome. > >Ian. > > > > > >Dr. Ian Montgomery, > >Histotechnology, > >I.B.L.S. Support Unit, > >Thomson Building, > >University of Glasgow, > >G12 8QQ. > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From rjbuesa <@t> yahoo.com Wed Feb 13 12:03:02 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 13 12:03:10 2008 Subject: [Histonet] Double-staining with same secondary antibody? In-Reply-To: <920cc4a70802130721k3207c80aodf72a786fd6b1242@mail.gmail.com> Message-ID: <115807.9268.qm@web61212.mail.yahoo.com> I think you will have no problems since your secondary can react with goat and mouse produced antibodies. The fact that the 1st requires HIER and the secondary does not is no problem either, the antigenic sites will be available for both. Using DAB-Nickel will give you the two colors you will need to distinguish both complexes (se 2ry Ab with conventional DAB). You should try it as described without buying any other reagents firts. Ren? J. Min-Han Tan wrote: --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From bdelescavage <@t> cellnetix.com Wed Feb 13 12:04:59 2008 From: bdelescavage <@t> cellnetix.com (Beth Delescavage) Date: Wed Feb 13 12:09:57 2008 Subject: [Histonet] Question about Detla CAL Message-ID: Hi All~ We received a sample of Delta CAL (gentle and fast acting decalcifier), has anyone used this? Do you like it? Does it work better than regular decal? Thanks for all your insight. ~Beth Beth Delescavage, BS, HTL (ASCP) QIHC CellNetix Laboratories Histotechnologist DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From rjbuesa <@t> yahoo.com Wed Feb 13 12:14:25 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 13 12:14:29 2008 Subject: [Histonet] Ethanol preserved tissues In-Reply-To: Message-ID: <959095.1118.qm@web61222.mail.yahoo.com> Those embryos where probably immersed in ethanol directly and probably their internal structure is not the best now. Your friend should determine the ethanol concentration they are now because, not matter how long they have been in ethanol, the embryos have to be completely dehydrate before processing and if the ethanol is 70% (as many museums use to) there is still some dehydraiton to do. Find the ethanol conc., take the embryos out, wash them well in clean ethanol of the same concentration they are now, and complete the dehydration up to 100% ethanol. I would not clear them in xylene because after so long in ethanol xylene will make them excessively brittle. I would try to clear the embryos in cedar wood oil until they are completely clear (transparent) and go to the bottom of the clearing container. After the dehydration, when your colleague places the embryos in the cedar wood oil, they will NOT sink, and you cannot permit them to hydrate again, so you cover the embryos (floating in the surface) with a piece of filter paper, and add 100% ethanol on top. This will prevent the embryos to hydrate. Once they sink (after clearing) place them in clean cedar wood oil for a few hours. Later "wash" them quickly with xylene and place them in the oven in melted paraffin (no more that 56?C) until infiltrate.Embed and section. Good luck! Ren? J. Laura Hunt wrote: --------------------------------- Never miss a thing. Make Yahoo your homepage. From rjbuesa <@t> yahoo.com Wed Feb 13 12:22:20 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 13 12:22:24 2008 Subject: [Histonet] (no subject) In-Reply-To: <005301c86e62$ac685c30$6424d182@IBLS.GLA.AC.UK> Message-ID: <359195.82702.qm@web61213.mail.yahoo.com> Ian: Fix your beasts in a mixture of 100 mL of absolute ethanol + 75 mL of chloroform + 25 mL of acetic acid and phenol to complete to 250 mL total. This fixative (described by Hetherington in 1922) will fix AND dehydrate your beats. Then immerse them in methyl salicylate and from there to paraffin wax. Ren? J. Ian Montgomery wrote: When the select few are sitting in the nuclear bunkers the dominant species on the planet will be nematodes. I've just started a project for my Zoologist colleagues studying these wee devils and I'm convinced they are the mega-organism. Formaldehyde, not a problem, couple of weeks later, give them a rinse and away they swim. Osmium tetroxide, "are they trying to annoy me with this slightly noxious compound." Managing to fix them is hard enough but processing for sectioning, a nightmare. Does anyone have experience processing these beasts? Hints and tips would be very welcome. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, G12 8QQ. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From PMonfils <@t> Lifespan.org Wed Feb 13 12:25:40 2008 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Feb 13 12:25:52 2008 Subject: [Histonet] Ethanol preserved tissues In-Reply-To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E273D20@LSRIEXCH1.lsmaster.lifespan.org> Though museums typically store their wet specimens in alcohol, some specimens may have been formalin fixed before storage in alcohol. If this is the case, the material may actually be better quality than if it had been stored for years in formalin. Is there any record of this? If the initial fixation and the long term storage were both in alcohol, then the cellular detail won't be as good as in material pre-fixed with formalin, but the basic tissue structure will still be there, and depending on what she is looking for she might get decent results. From lesley.bechtold <@t> jax.org Wed Feb 13 12:58:42 2008 From: lesley.bechtold <@t> jax.org (Lesley Bechtold) Date: Wed Feb 13 12:59:24 2008 Subject: [Histonet] Histology position in Bar Harbor, Maine! Message-ID: <20080213135842148.00000002468@spikey> Hi Everyone, We have an opening in beautiful Bar Harbor, Maine. The information is: There is a fulltime position in the Histology Service of The Jackson Laboratory as a Histotechnologist II/III. Responsibilities include conduct of standard histological protocols including embedding, sectioning and staining as well as routine laboratory maintenance and administrative tasks. Minimum qualifications include Associate's degree in a biological science and HT(ASCP) certification plus 3-5 years of experience in histology OR a Bachelor's degree in a biological field and a minimum 3 years of experience in histology. Experience in murine histology and specialized techniques such as serial sectioning, immunohistochemistry, plastic embedding and plastic sectioning is helpful. The incumbent will have the opportunity to further their skills and knowledge. Required computer skills include email, internet, word processing, spreadsheets and familiarity with databases. Effective written and verbal communication skills are essential. Successful applicants will demonstrate good interpersonal skills and must have the ability and willingness to function effectively in a team environment. The Jackson Laboratory is one of the world's foremost centers for mammalian genetics research. Located in Bar Harbor, Maine, the lab is adjacent to Acadia National Park. Mountains, ocean, forests, lakes, and trails are all within walking distance. If you are looking for a more natural environment, this could be the opportunity you've been searching for. Interested individuals should apply on-line on the internet at www.jax.org, click on Careers in the green bar at the top of the page, then on Search Job Listings in the right-hand column. Refer to job requisition #1127. Please submit cover letter and resume as one document. Lesley S. Bechtold Senior Manager, Histopathology & Microscopy Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 From Farnana <@t> nehealth.com Wed Feb 13 13:00:03 2008 From: Farnana <@t> nehealth.com (Amy Farnan) Date: Wed Feb 13 13:00:16 2008 Subject: [Histonet] OPEN FULL TIME HISTOLOGY POSITION Message-ID: <47B2F7E4.26ED.00D9.0@nehealth.com> Hi all - I have a full time histotechnician position available in Springfield, Mass. This is a small histology lab within a urology office. We are willing to hire a fulltime or 2 or 3 part timers. Responsibilities include microwave processing, embedding, cutting and staining prostate biopsies. Pay is very competitive. For more information please contact: Amy Farnan, HT(ASCP) Twin Crest Pathology Consultant a.farnan@yahoo.com 518-527-7073 Disclaimer: The information in this message is confidential. If you are not the intended recipient, do not disclose, copy, or distribute this message, and please immediately contact the sender. From laurie.colbert <@t> huntingtonhospital.com Wed Feb 13 13:19:17 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed Feb 13 13:19:27 2008 Subject: [Histonet] Cassette Storage Message-ID: <57BE698966D5C54EAE8612E8941D76830267DF89@EXCHANGE3.huntingtonhospital.com> Does anyone know if the "Tissue Tek Embedding System" cassette holders still exist? They are the plastic containers (boxes) that have a tilt-out door in the front. We use them in the grossing room for storage and easy access to cassettes at the grossing station. I have seen similar products from other companies, but the dimensions are never quite right. The ones I use are 8 ? " wide, 8 ? " high, and 7" deep. From ksecrest <@t> hsc.wvu.edu Wed Feb 13 14:12:39 2008 From: ksecrest <@t> hsc.wvu.edu (Kimberly Secrest) Date: Wed Feb 13 14:13:40 2008 Subject: [Histonet] IK1/2 antibody Message-ID: <47B308F2.C068.0078.0@hsc.wvu.edu> Hello, Has anyone had any experience working with the rabbit polyclonal IK1/2 antibody? The researcher would like to use it on formalin fixed paraffin embedded mouse tissue. Any feedback would be greatly appreciated. Thank you Kim Kimberly Secrest, HTL, QIHC Tissue Bank and Translational Research Lab Administrator West Virginia University 304-293-7628 From trinimaican2501 <@t> yahoo.com Wed Feb 13 14:23:53 2008 From: trinimaican2501 <@t> yahoo.com (I-sanna Gibbons) Date: Wed Feb 13 14:23:58 2008 Subject: [Histonet] snail - shell and all Message-ID: <357917.76648.qm@web50311.mail.re2.yahoo.com> Hi all, The necropsy technician is having a bit of trouble processing an entire snail. Decalcifying the shell seems to be very difficult. Has anyone ever done this before and has a complete processing schedule he can follow? Any advice is welcomed! Thanks I-sanna Gibbons DVM ____________________________________________________________________________________ Never miss a thing. Make Yahoo your home page. http://www.yahoo.com/r/hs From rjbuesa <@t> yahoo.com Wed Feb 13 14:31:40 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 13 14:31:44 2008 Subject: [Histonet] Cassette Storage In-Reply-To: <57BE698966D5C54EAE8612E8941D76830267DF89@EXCHANGE3.huntingtonhospital.com> Message-ID: <847369.34096.qm@web61224.mail.yahoo.com> Try Sakura FineTek. They bought Tissue Tek many years ago and probebly still make these storage units. Ren? J. Laurie Colbert wrote: Does anyone know if the "Tissue Tek Embedding System" cassette holders still exist? They are the plastic containers (boxes) that have a tilt-out door in the front. We use them in the grossing room for storage and easy access to cassettes at the grossing station. I have seen similar products from other companies, but the dimensions are never quite right. The ones I use are 8 ? " wide, 8 ? " high, and 7" deep. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From alonso.martinezcanabal <@t> utoronto.ca Wed Feb 13 14:33:00 2008 From: alonso.martinezcanabal <@t> utoronto.ca (Alonso Martinez-Canabal) Date: Wed Feb 13 14:33:58 2008 Subject: [Histonet] Vibratome Slicing In-Reply-To: <357917.76648.qm@web50311.mail.re2.yahoo.com> References: <357917.76648.qm@web50311.mail.re2.yahoo.com> Message-ID: <4DCEFDFCE5BB43E08DC15B1CB8C63C15@Astrocito> Dear Everyone, Hi, I have been slicing Mouse brains (fixed with PFA) in vibratome, embedded in agarose. I am not keeping the sections in free floating, I am mounting immediately. But After I let the sections dry and start to process them for IHC, some weird bubbles appear in the sections, does someone knows what could be happening? Thank You very much, Alonso From kellerc2 <@t> uthscsa.edu Wed Feb 13 14:51:16 2008 From: kellerc2 <@t> uthscsa.edu (Keller, Charles) Date: Wed Feb 13 14:51:25 2008 Subject: [Histonet] snail - shell and all In-Reply-To: <357917.76648.qm@web50311.mail.re2.yahoo.com> References: <357917.76648.qm@web50311.mail.re2.yahoo.com> Message-ID: I'm happy to do microCT-based virtual histology for your colleague at no cost. It doesn't require sectioning, but you can still section it later. Sincerely, Charles Charles Keller, MD Assistant Professor, Department of Cellular & Structural Biology Adjunct Assistant Professor, Department of Pediatrics Director, Small Animal Imaging Facility Greehey Children's Cancer Research Institute The University of Texas Health Science Center 8403 Floyd Curl Drive, Mail Code 7784 San Antonio, TX 78229-3900 210-562-9062 [office] 210-562-9014 [fax] http://gccri.uthscsa.edu or www.sarcomalab.org kellerc2@uthscsa.edu Postdoctoral Opportunities and Undergraduate & Summer Internships: http://ccri.uthscsa.edu/keller CCRI and UTHSCSA wish to promote open communication while protecting confidential and/or privileged information. If you have received this message in error, please inform the sender and delete all copies. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of I-sanna Gibbons Sent: Wednesday, February 13, 2008 2:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] snail - shell and all Hi all, The necropsy technician is having a bit of trouble processing an entire snail. Decalcifying the shell seems to be very difficult. Has anyone ever done this before and has a complete processing schedule he can follow? Any advice is welcomed! Thanks I-sanna Gibbons DVM ________________________________________________________________________ ____________ Never miss a thing. Make Yahoo your home page. http://www.yahoo.com/r/hs _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From juditw <@t> u.washington.edu Wed Feb 13 15:05:33 2008 From: juditw <@t> u.washington.edu (Judith L. Williams) Date: Wed Feb 13 15:05:45 2008 Subject: [Histonet] (no subject) In-Reply-To: Message-ID: Hi to Ian- Great advice and links from Phil- having worked on the second most abundant form of meiofauna- the copepods- and done light level, EM and confocal on them - fixation is a problem for sure. there are some elegant morphology studies from the 70;s and again - most are EM. good luck - try looking up meiofauna morphology on google or nematode morphology for the basics. best! Judy at U. of Washington On Wed, 13 Feb 2008, Philip Oshel wrote: > Ian, > > A couple of useful web sites: > http://www.wormatlas.org/ > and Dave Hall's lab at Albert Einstein Coll. of Medicine: > http://wormem.aecom.yu.edu/ > Jay Campbell at U Wisconsin has done a bunch of C. elegans EM. > jmcampbe@wisc.edu > Mind, most of the people I know are either doing EM or confocal on whole-mount > or squish-mount worms. Not light microscopic sections, so I'm not sure how > useful these contacts will be. > The big trick for fixation & processing at the EM level is high-pressure > freezing followed by freeze-substitution for embedding. > For "regular" light histology & sectioning, I'd suggest lopping off the head & > tail & maybe bisecting the body, then embed in JB-4 or similar resin and cut > with a glass knife. > > Paraffin embedding and steel knives won't cut it. > > Phil > > >> When the select few are sitting in the nuclear bunkers the >> dominant species on the planet will be nematodes. I've just started a >> project for my Zoologist colleagues studying these wee devils and I'm >> convinced they are the mega-organism. Formaldehyde, not a problem, couple of >> weeks later, give them a rinse and away they swim. Osmium tetroxide, "are >> they trying to annoy me with this slightly noxious compound." Managing to >> fix them is hard enough but processing for sectioning, a nightmare. Does >> anyone have experience processing these beasts? Hints and tips would be very >> welcome. >> >> Ian. >> >> Dr. Ian Montgomery, >> Histotechnology, >> I.B.L.S. Support Unit, >> Thomson Building, >> University of Glasgow, >> G12 8QQ. > -- > Philip Oshel > Microscopy Facility Supervisor > Biology Department > 024C Brooks Hall > Central Michigan University > Mt. Pleasant, MI 48859 > (989) 774-3576 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Lia.Caldwell <@t> TriadHospitals.com Wed Feb 13 18:24:13 2008 From: Lia.Caldwell <@t> TriadHospitals.com (Caldwell, Lia) Date: Wed Feb 13 18:24:33 2008 Subject: [Histonet] PAS w/wout Diastase liver controls Message-ID: Hey all, Just wondering if I could pick your brains about the inconsistency of the PAS with and without Diastase method on liver controls. We cut several unstained slides on our liver patient panels, and if they happen to have a positive reaction, we will keep the unstained slides to use as future controls. We also have a liver control that was once very good for this method. We have noticed that, after time, the PAS reaction weakens on the PAS w/out D on both controls. Our results are not consistent regardless if you use saliva or Diastase of Malt & buffer. Sometimes the patient's stain is better than the control that was beautiful weeks before. Can this be attributed to the continual breakdown of glycogen in the liver even after processing? Does anyone have solid research to indicate tissue/substance degeneration long after processing? Any information would help. Thank you! ~Lia Lia M. Caldwell HT (ASCP) Histology Supervisor Oro Valley Pathology Dept. phone: (520) 901-3914 www.Lia.Caldwell@TriadHospitals.com From akemiat3377 <@t> yahoo.com Wed Feb 13 19:28:13 2008 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Wed Feb 13 19:28:17 2008 Subject: [Histonet] PAS w/wout Diastase liver controls In-Reply-To: Message-ID: <806658.30308.qm@web31307.mail.mud.yahoo.com> Hi Lia, I also had similar experiences years ago using liver w/ & w/o for PAS. Have you ever tried using a Cervix (including both endocervix and ectocervix) as a control? It is also considered to be an excellent control. Akemi --- "Caldwell, Lia" wrote: > Hey all, > Just wondering if I could pick your brains about the > inconsistency of the PAS with and without Diastase > method on liver controls. We cut several unstained > slides on our liver patient panels, and if they > happen to have a positive reaction, we will keep the > unstained slides to use as future controls. We also > have a liver control that was once very good for > this method. We have noticed that, after time, the > PAS reaction weakens on the PAS w/out D on both > controls. Our results are not consistent regardless > if you use saliva or Diastase of Malt & buffer. > Sometimes the patient's stain is better than the > control that was beautiful weeks before. Can this > be attributed to the continual breakdown of glycogen > in the liver even after processing? Does anyone > have solid research to indicate tissue/substance > degeneration long after processing? Any information > would help. Thank you! > ~Lia > > Lia M. Caldwell HT (ASCP) > Histology Supervisor > Oro Valley Pathology Dept. > phone: (520) 901-3914 > www.Lia.Caldwell@TriadHospitals.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Akemi Allison-Tacha, BS, HT(ASCP)HTL Client Services Manager PhenoPath laboratories 551 North 34th Street, Suite 100 Seattle, WA 98103-8675 Work: (206) 374-9000 Cell: (425) 941-4287 E-Mail: akemiat3377@yahoo.com From darkmaya <@t> gmail.com Wed Feb 13 20:03:15 2008 From: darkmaya <@t> gmail.com (Leigh Propper) Date: Wed Feb 13 20:03:25 2008 Subject: [Histonet] First Post and a Question Message-ID: Hi everyone I just joined the histonet not too long ago. I finished my Histology program last autumn and I am sitting my ASCP HTL in a little over a week. I am very nervous! I have studied and studied and I still feel like I am missing something. Has anyone on here taken the HTL recently since it has been changed, and can share their experience with the types of questions asked? I've just been told "It's scary and hard!"! Thanks for any input. What a wonderful resource this place is! Its so nice reading all of the information, one never knows when it may come in handy. Thanks, Leigh From rydomsal <@t> yahoo.com Wed Feb 13 20:54:07 2008 From: rydomsal <@t> yahoo.com (Ryan Dominique Salazar) Date: Wed Feb 13 20:54:11 2008 Subject: [Histonet] Perl's Prussian blue Iron stain Message-ID: <188464.28830.qm@web52306.mail.re2.yahoo.com> Hi everyone, Please help me on Iron staining. These are my questions: -do I need to stain quickly after heating in the hot plate? -very pale nuclear fast red (Sigma) stain. -Is this OK for my working solution? Hydrochloric Acid-Potassium Ferrocyanide Solution 2% hydrochloric acid -------------------------------------------- 20.0 ml 1% potassium ferrocyanide ------------------------------------- 20.0 ml Prepare just before use and discard after use. -my positive control is liver, but it seems the stain is pale, do I need to change my procedure? 3 changes of xylene (3 min each) 3 changes of abs. ETOH (3 min @) 70% ETOH 80% ETOH HCl-K Ferrocyanide Working solution (30 min) 5 changes of distilled water Nuclear fast red (3 min) 3 changes of distilled water 70% ETOH 3 dips 80% ETOH 3 dips 3 changes of Abs. ETOH (3 dips @) 3 changes of xylene (3 dips each) Mount with entellan -Please provide for me any procedures/techniques Thanks fo your big help. Your advice regarding the brain tissue section works well. --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From ccrowder <@t> vetmed.lsu.edu Wed Feb 13 22:19:51 2008 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Wed Feb 13 22:21:59 2008 Subject: [Histonet] Contract Lab needed Message-ID: Hi - We have a researcher who has about 700 cassettes (mice) which need processing and cutting for H & E. Does anyone know of a contract lab that can do this work? Thanking you in advance for the info, Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 From rjbuesa <@t> yahoo.com Thu Feb 14 07:42:36 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 14 07:42:40 2008 Subject: [Histonet] snail - shell and all In-Reply-To: <357917.76648.qm@web50311.mail.re2.yahoo.com> Message-ID: <918335.23971.qm@web61224.mail.yahoo.com> That will be extremely difficult because, even when the calcium is eliminated, tehre is a tough matrix that has to be dealt with. Why would you like (need) to do that? Ren? J. I-sanna Gibbons wrote: Hi all, The necropsy technician is having a bit of trouble processing an entire snail. Decalcifying the shell seems to be very difficult. Has anyone ever done this before and has a complete processing schedule he can follow? Any advice is welcomed! Thanks I-sanna Gibbons DVM ____________________________________________________________________________________ Never miss a thing. Make Yahoo your home page. http://www.yahoo.com/r/hs _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From rjbuesa <@t> yahoo.com Thu Feb 14 07:45:41 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 14 07:45:45 2008 Subject: [Histonet] PAS w/wout Diastase liver controls In-Reply-To: Message-ID: <907677.54663.qm@web61213.mail.yahoo.com> Your assumption is correct. The solution is to not cut/store an excessive amount of control slides. Just keep one week supply and you will be better off (blame the air oxygen contents). Ren? J. "Caldwell, Lia" wrote: --------------------------------- Never miss a thing. Make Yahoo your homepage. From histology.bc <@t> shaw.ca Thu Feb 14 09:01:19 2008 From: histology.bc <@t> shaw.ca (Paul Bradbury) Date: Thu Feb 14 08:57:57 2008 Subject: [Histonet] Perl's Prussian blue Iron stain In-Reply-To: <188464.28830.qm@web52306.mail.re2.yahoo.com> References: <188464.28830.qm@web52306.mail.re2.yahoo.com> Message-ID: <47B457BF.2000201@shaw.ca> Hi Ryan, A couple of comments regarding iron "staining". First, the demonstration of iron using the Perls' procedure is not a staining technique. It is a histochemical reaction between the ferric ions and potassium ferro cyanide, the visible end product is ferric ferro cyanide (also known as Prussian blue). This end product is permanent and will not fade under normal conditions. Iron in any form in blocks and sections is remarkably stable. You can store sections for years, or you can stain them immediately after drying following cutting. Nuclear fast red is an inherently pale nuclear stain. It is popular as a counterstain in the Perls' procedure because it is so unlikely to mask any very fine iron reactions. Some of the other red nuclear stain, such as neutral red or saffranin, are more potent and will produce much more non-nuclear coloration unless they are washed throughly after staining. If your control is liver, you may anticipate pale reactions all round. Liver generally, unless it is from a case involving an iron-storage disease, has very little demonstrable iron. Also, the nuclei of hepatocytes stain very pale as their chromatin is very diffuse. Lastly, regarding your staining procedure. I would suggest the following: Xylene - 2 changes - 3 minutes each 100% Alcohol - 3 changes - 1 minute each 95% alcohol - 1 change - 1 minute Distilled water - 2 changes - 1 minutes each (to remove any contaminant iron from other sources) Ferrocyanide/HCl working solution - 20 minutes Distilled water - 2 changes - 1 minute each Continue with the other steps that you currently use, they are just fine. Paul Bradbury, Kamloops, Canada Ryan Dominique Salazar wrote: > Hi everyone, > > Please help me on Iron staining. These are my questions: > > -do I need to stain quickly after heating in the hot plate? > -very pale nuclear fast red (Sigma) stain. > -Is this OK for my working solution? > Hydrochloric Acid-Potassium Ferrocyanide Solution > 2% hydrochloric acid -------------------------------------------- 20.0 ml > 1% potassium ferrocyanide ------------------------------------- 20.0 ml > > Prepare just before use and discard after use. > -my positive control is liver, but it seems the stain is pale, do I need to change my procedure? > 3 changes of xylene (3 min each) > 3 changes of abs. ETOH (3 min @) > 70% ETOH > 80% ETOH > HCl-K Ferrocyanide Working solution (30 min) > 5 changes of distilled water > Nuclear fast red (3 min) > 3 changes of distilled water > 70% ETOH 3 dips > 80% ETOH 3 dips > 3 changes of Abs. ETOH (3 dips @) > 3 changes of xylene (3 dips each) > Mount with entellan > > -Please provide for me any procedures/techniques > > Thanks fo your big help. Your advice regarding the brain tissue section works well. > > > > > > --------------------------------- > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From Maxim_71 <@t> mail.ru Thu Feb 14 09:37:32 2008 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Thu Feb 14 09:39:23 2008 Subject: [Histonet] a reference for Streptavidin-biotin detecting system Message-ID: <179832857.20080214183732@mail.ru> Dako "Handbook on Immunohistochemical staining methods" (4 ed) contains detail info about all detections system, which manufactured DakoCytomation. This book is available on website Dako. Maxim Peshkov. K M wrote: Dear Histonetters I am writting a paper in related to IHC. I will use streptavidin-biotin antigen detecting method. I have to put a reference for that method. Anyone tell me a title of a textbook , pages, publisher of that textbook in which this method mentioned. Thanks in advance Khalaf B. M.B.B.S Egypty From PMonfils <@t> Lifespan.org Thu Feb 14 09:56:31 2008 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Feb 14 09:56:37 2008 Subject: [Histonet] snail - shell and all In-Reply-To: <357917.76648.qm@web50311.mail.re2.yahoo.com> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273D21@LSRIEXCH1.lsmaster.lifespan.org> You can't decalcify a snail shell. You can decalcify a bone because there is a fibrous matrix which is infiltrated with calcium salts. But a snail shell has no such matrix. It is almost 100% calcium salts. If you decalcify it, you simply dissolve it. There would be nothing left at all. From Beatrice.Debrosse-Serra <@t> pfizer.com Thu Feb 14 10:16:29 2008 From: Beatrice.Debrosse-Serra <@t> pfizer.com (Debrosse-Serra, Beatrice) Date: Thu Feb 14 10:16:46 2008 Subject: [Histonet] Question about Detla CAL In-Reply-To: Message-ID: <8404DFBED5207B4B8E5EEF4332CEEA5305DE9206@lajamrexm01.amer.pfizer.com> I like this Decal a lot. They have two products, DeltaCal, which works just as well than anything else I have used and the DeltaCal lite, which takes longer to decalcify, but it is much gentler. Besides, the Customer Service from Delta Medical is wonderful! Beatrice DeBrosse-Serra Pathology Scientist Pfizer Global Research & Development CB4, 2150 10646 Science Center Drive San Diego, CA 92121 Phone# 858-622-5986 Fax# 858-678-8290 From ccrowder <@t> vetmed.lsu.edu Thu Feb 14 10:34:37 2008 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Thu Feb 14 10:39:32 2008 Subject: [Histonet] Contract lab Message-ID: I want to thank all of you who responded so quickly to my need for a contract lab. I have been overwhelmed. Don't know what I'd do without you. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 From nancy.troiano <@t> yale.edu Thu Feb 14 10:57:12 2008 From: nancy.troiano <@t> yale.edu (Nancy W. Troiano) Date: Thu Feb 14 10:57:22 2008 Subject: [Histonet] Snail shell Message-ID: <5.2.1.1.2.20080214115626.00c50348@email.med.yale.edu> Why not embed the snail in MMA (methylmethacrylate) and cut it without decalcifying? My guess is that you would get some beautiful histology with this method. From histology.bc <@t> shaw.ca Thu Feb 14 11:02:38 2008 From: histology.bc <@t> shaw.ca (Paul Bradbury) Date: Thu Feb 14 10:59:18 2008 Subject: [Histonet] Perl's Prussian blue Iron stain In-Reply-To: <653649.21671.qm@web61221.mail.yahoo.com> References: <653649.21671.qm@web61221.mail.yahoo.com> Message-ID: <47B4742E.4020505@shaw.ca> Hi Ren?, You make a valid point, however, although all staining reactions are histochemical reactions, NOT all histochemical reactions are staining reactions. Some of this argument may seem to be too concerned with semantics, but in order to have a full understanding of how tissue demonstration techniques work, a precise knowledge of the reactions involved is essential. A "staining reaction" involves bonding (in one form another) between the auxochromic groups of the dye molecule and the available reactive groups of the tissue. The demonstration of iron, by Perls' method, or Turnbull's method, is purely a chemical reaction between reactive compounds. It is an inorganic chemistry reaction, and the tissue itself plays no role in the formation of the final visible product. Exactly the same reaction may be seen by combining a ferric iron solution with potassium ferrocyanide in a glass tube. Many people routinely talk about "staining" a tissue component. However, several commonly used histological procedures are not true "staining" reactions. The ferric and ferrous iron "stains", Colloidal iron for sulphated mucins, von Kossa for calcium (actually phosphates, carbonates), the silver "stains" used for reticulin, fungi, axons, dendrites, etc, oil red O for neutral lipids, periodic acid Schiff (PAS) reaction, enzyme demonstration techniques, and immunohistochemical techniques are all histochemical techniques, but none of them are staining techniques. Paul Kamloops, Canada 'Rene J Buesa wrote: > Dear Paul: > Aren't ALL staining techniques histochemical reactions as well? Many > have poorly understood mechanisms but ALL HC stains are HC reactions. > Ren? J. > > */Paul Bradbury /* wrote: > > > > > > ------------------------------------------------------------------------ > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try > it now. > From liz <@t> premierlab.com Thu Feb 14 11:31:53 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Feb 14 11:31:56 2008 Subject: [Histonet] vitronectin in porcine tissues Message-ID: Hello everyone and happy valentines day. Is anyone out there aware of a antibody to vitronectin that works in porcine tissue samples. I've done a bit a searching and I can't seem to find any documentation with regards to porcine. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 From erika.harrell <@t> teamstaffrx.com Thu Feb 14 11:47:39 2008 From: erika.harrell <@t> teamstaffrx.com (Erika Harrell) Date: Thu Feb 14 11:46:57 2008 Subject: [Histonet] Histo position in Rhode Island Message-ID: <1137600.1203011259569.JavaMail.cfservice@webserver18> Histo Community, We have an opening for a Temporary Histotech in Rhode Island. Level 1 Trauma center, Mon-Fri, no weekends. Helpful to have experience with Dako Stainer. Experience in a Medium to Large size hospital, trauma center preferred. Rhode Island State licensure required, as well as ASCP certification. If you're interested please contact Lindsay Zilai at 877.523.9897 ext 5485 or email her directly at lindsay.zilai@teamstaffrx.com Erika Harrell | Recruiter - Permanent Placement | TeamStaff Rx, Inc. Tel: 1.877.523.9897 ext 5475 | Fax: 1.866.365.6566 | www.teamstaffrx.com America?s Winning Healthcare Staffing Solution This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential or exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that you are strictly prohibited from printing, storing, disseminating, distributing or copying this communication. If you have received this communication in error, please notify the sender immediately by replying to the message and deleting it from your computer. Thank you. From kbradshaw <@t> lcpath.com Thu Feb 14 11:57:13 2008 From: kbradshaw <@t> lcpath.com (Kari Bradshaw) Date: Thu Feb 14 11:57:19 2008 Subject: [Histonet] Microwave processor users Message-ID: <6b37a5fe5709a64bb77219b88113cf06@mail2.lcpath.com> Hi everyone, I am looking for contacts who can share basic operating instructions and protocols with me. We obtained a used H2800 Energy Beam Microwave Processor and need some help to get it up and running. Please contact me directly. Thanks, Kari Bradshaw Anatomic Pathology Manager Lower Columbia Pathologists 1217 14th Ave Longview, WA 98632 360.425.5620 kbradshaw@lcpath.com From Clifton.Kari <@t> mayo.edu Thu Feb 14 12:01:29 2008 From: Clifton.Kari <@t> mayo.edu (Clifton, Kari B., Ph.D.) Date: Thu Feb 14 12:01:39 2008 Subject: [Histonet] zinc formalin Message-ID: We will be doing some IHC to detect BrdU in rate bone and some soft tissues. Based on the recommendations from folks on the list, and from reading through the archives, I have ordered the concentrated zinc formalin from Anatech. A question to clarify-- do you recommend using a 10% solution, as standard with regular NBF? That is, dilute the 5X concentrate to 1X, then dilute some to 10%? Thanks for your help, Kari From esther.peters <@t> verizon.net Thu Feb 14 12:02:25 2008 From: esther.peters <@t> verizon.net (Esther Peters) Date: Thu Feb 14 12:03:42 2008 Subject: [Histonet] snail - shell and all In-Reply-To: <918335.23971.qm@web61224.mail.yahoo.com> References: <918335.23971.qm@web61224.mail.yahoo.com> Message-ID: <47B48231.6000301@verizon.net> Rene is correct, there should be a proteinaceous matrix left. Some people decalcify the fixed snail in the shell, because removing the animal is also very difficult! But you should be able to trim the animal after the shell is decalcified, and if it is difficult to section, use Nair or another softening procedure on the block. What kind of decal are you using? For how long? How big (what species) is the snail? Esther Peters, Ph.D. George Mason University Rene J Buesa wrote: > That will be extremely difficult because, even when the calcium is eliminated, tehre is a tough matrix that has to be dealt with. Why would you like (need) to do that? > Ren? J. > > I-sanna Gibbons wrote: Hi all, > The necropsy technician is having a bit of trouble processing an entire snail. Decalcifying the shell seems to be very difficult. Has anyone ever done this before and has a complete processing schedule he can follow? > > Any advice is welcomed! > > Thanks > I-sanna Gibbons DVM > > > ____________________________________________________________________________________ > Never miss a thing. Make Yahoo your home page. > http://www.yahoo.com/r/hs > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Janet.Bonner <@t> FLHOSP.ORG Thu Feb 14 12:16:35 2008 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Thu Feb 14 12:17:50 2008 Subject: [Histonet] nematodes References: <005301c86e62$ac685c30$6424d182@IBLS.GLA.AC.UK> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2502@fhosxchmb006.ADVENTISTCORP.NET> Some good, full-strength Ethanol may slow them down a bit!!!! (It does me) ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Ian Montgomery Sent: Wed 2/13/2008 12:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) When the select few are sitting in the nuclear bunkers the dominant species on the planet will be nematodes. I've just started a project for my Zoologist colleagues studying these wee devils and I'm convinced they are the mega-organism. Formaldehyde, not a problem, couple of weeks later, give them a rinse and away they swim. Osmium tetroxide, "are they trying to annoy me with this slightly noxious compound." Managing to fix them is hard enough but processing for sectioning, a nightmare. Does anyone have experience processing these beasts? Hints and tips would be very welcome. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, G12 8QQ. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From maa8 <@t> cornell.edu Thu Feb 14 12:40:29 2008 From: maa8 <@t> cornell.edu (Mary Ascenzi) Date: Thu Feb 14 12:38:51 2008 Subject: [Histonet] Lac Z gene and GFP Message-ID: <5.2.1.1.2.20080214131220.00ab4fb0@postoffice6.mail.cornell.edu> Hello all We are going to inject a plasmid along with a reporter gene into muscle. The plasmid that is transfected and its expression is detected after a few days by identifying the protein coded by the reporter gene. One of the reporter genes that will be used is the Lac Z gene (beta-galactosidase) and it is identified using X-gal (5-bromo-4-chloro-3-indoyl-beta-D-galactopyranosdie). The other is Green Fluorescent Protein (GFP) which is identified by fluorescence microscopy. What we need are methods to detect Lac Z and GFP in muscle tissue after the transfections. Which kits have worked for you? Thanks in advance, Mary Ascenzi From llewllew <@t> shaw.ca Thu Feb 14 12:45:53 2008 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Thu Feb 14 12:46:21 2008 Subject: [Histonet] Hawaii Message-ID: <002d01c86f39$d3cff760$4e184246@yourlk4rlmsu> If there is a histotech who lives in Hawaii and who would consider doing me a favour, all legal and above board, could you please contact me off list. It may involve some photography. Thanks, Bryan Llewellyn From mtitford <@t> aol.com Thu Feb 14 12:52:22 2008 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Thu Feb 14 12:52:39 2008 Subject: [Histonet] Cassette dispensor. Message-ID: <8CA3D51DE0A2131-F68-CB9@WEBMAIL-DF12.sysops.aol.com> Laurie Colbert askes about the old "Tissue Tek Embedding System" cassette dispensors. We purchased some of those?years ago when they first came out. I saw them at the NSH meeting in Little Rock in 1986 (I think)?and bought them right after. When I went to purchase some more about 1990, they were already "done gone" (No longer available). I think most people now just open the little window on the box the cassettes come in, and use them that way. Not so pretty, and not so easy to keep clean! Michael Titford Pathology USA Mobile AL USA ________________________________________________________________________ More new features than ever. Check out the new AOL Mail ! - http://webmail.aol.com From MElliott <@t> mrl.ubc.ca Thu Feb 14 13:08:39 2008 From: MElliott <@t> mrl.ubc.ca (Mark Elliott) Date: Thu Feb 14 13:09:32 2008 Subject: [Histonet] HOPE Solution In-Reply-To: References: Message-ID: <47B42137020000D60002C32F@mail.mrl.ubc.ca> Has anyone ever worked with this? HOPE (Hepes-Glutamic acid buffer mediated Organic solvent Protection Effect) solution Any recipes? Thanks Mark ***CONFIDENTIALITY NOTICE*** This electronic message is intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From JWEEMS <@t> sjha.org Thu Feb 14 13:23:39 2008 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Thu Feb 14 13:23:46 2008 Subject: [Histonet] Cassette dispensor. In-Reply-To: <8CA3D51DE0A2131-F68-CB9@WEBMAIL-DF12.sysops.aol.com> References: <8CA3D51DE0A2131-F68-CB9@WEBMAIL-DF12.sysops.aol.com> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD320518E676@sjhaexc02.sjha.org> Look at MarketLab... I believe they have something like this. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mtitford@aol.com Sent: Thursday, February 14, 2008 1:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette dispensor. Laurie Colbert askes about the old "Tissue Tek Embedding System" cassette dispensors. We purchased some of those?years ago when they first came out. I saw them at the NSH meeting in Little Rock in 1986 (I think)?and bought them right after. When I went to purchase some more about 1990, they were already "done gone" (No longer available). I think most people now just open the little window on the box the cassettes come in, and use them that way. Not so pretty, and not so easy to keep clean! Michael Titford Pathology USA Mobile AL USA ________________________________________________________________________ More new features than ever. Check out the new AOL Mail ! - http://webmail.aol.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From bakevictoria <@t> gmail.com Thu Feb 14 18:45:08 2008 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Thu Feb 14 18:45:13 2008 Subject: [Histonet] little things in histology In-Reply-To: References: Message-ID: <4f016b690802141645s1cbc9c0fw97cd26efb283b4e9@mail.gmail.com> Debbie, I've been using Amonia water for a long time, but with caution, it isn't necessary for all tissues and the strength of the solution along with the length of time could affect the H&E staining. A general solution strength is between .25 to 1.25% and for no longer than five minutes (depending on the tissue type) should be done. All slides should be drained of any excess water (when using plus slides or coated slides - scoring of the paraffin around the tissue will help release any trapped water) before heating. Excess water under the section will result in tissue loss and staining issues. Vikki On 2/12/08, WWmn916@aol.com wrote: > hello everyone > > two little detail questions > 1) could soaking prefaced paraffin blocks a little too long in ammonia water > effect the h+e staining of that slide causing a muddy h+e? > 2)could leaving slides in 65-70 degree oven (about 30-45 minutes or longer) > cause heat artifact and muddy the h+e stain? > > thanks > deb king > > > > **************The year's hottest artists on the red carpet at the Grammy > Awards. Go to AOL Music. > (http://music.aol.com/grammys?NCID=aolcmp00300000002565) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tgoodpas <@t> fhcrc.org Thu Feb 14 19:30:40 2008 From: tgoodpas <@t> fhcrc.org (Goodpaster, Tracy A) Date: Thu Feb 14 19:30:45 2008 Subject: [Histonet] Contract Lab needed In-Reply-To: References: Message-ID: Hi Cheryl, We routinely process, embed, section and stain different species. We are a resource lab at Fred Hutchinson Cancer Research Center and provide services for researchers at this institution, as well as other organizations in our local area and across the country. The majority of our work is with mouse and human tissue but we have also optimized protocols for other species. We have six full-time ASCP certified techs from a wide background and we offer a very high quality of work. If we can be of any help, please don't hesitate to contact me. Thank you, Tracy Goodpaster -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Crowder Sent: Wednesday, February 13, 2008 8:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Contract Lab needed Hi - We have a researcher who has about 700 cassettes (mice) which need processing and cutting for H & E. Does anyone know of a contract lab that can do this work? Thanking you in advance for the info, Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tgoodpas <@t> fhcrc.org Thu Feb 14 19:47:55 2008 From: tgoodpas <@t> fhcrc.org (Goodpaster, Tracy A) Date: Thu Feb 14 19:47:58 2008 Subject: [Histonet] Contract lab In-Reply-To: References: Message-ID: Hi Cheryl, We routinely process, embed, section and stain different species. We are a resource lab at Fred Hutchinson Cancer Research Center and provide services for researchers at this institution, as well as other organizations in our local area and across the country. The majority of our work is with mouse and human tissue but we have also optimized protocols for other species. We have six full-time ASCP certified techs from a wide background and we offer a very high quality of work. If we can be of any help, please don't hesitate to contact me. Thank you, Tracy Goodpaster Tracy Goodpaster, HTL (ASCP) QIHC Fred Hutchinson Cancer Research Center PO Box 19024 / M5 A803 Seattle, WA 98109-1024 (206) 667-6118 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Crowder Sent: Thursday, February 14, 2008 8:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Contract lab I want to thank all of you who responded so quickly to my need for a contract lab. I have been overwhelmed. Don't know what I'd do without you. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Thu Feb 14 20:36:22 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Thu Feb 14 20:36:26 2008 Subject: [Histonet] PAS + IF Message-ID: <582736990802141836u25754898n733d083325354a31@mail.gmail.com> Hi, There is a researcher here that is interested in doing a PAS and an immunofluorescent label on the same slide. She is using formalin fixed paraffin embedded tissue and the antibody requires heat induced epitope retrieval. So obviously the PAS will need to come first as the reagents involved would innevitably destroy the fluorescent label. The question is: will the retrieval affect the PAS reaction? My initial guess was that the PAS is a fairly strong bond (spill schiffs on your hand ... see what I mean!) so it should survive, but that is just a guess. Has anyone tried it? Thanks, Amos Brooks From amosbrooks <@t> gmail.com Thu Feb 14 20:42:35 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Thu Feb 14 20:42:39 2008 Subject: [Histonet] Lobster Processing on VIP 2000 Message-ID: <582736990802141842o131e51edha8ff329fb7193541@mail.gmail.com> Hi again, Question #2: There is a local college that has just gotten a VIP 2000 and is having some trouble using it. I would like to help, but I've not used this particular model in many years. Does anyone happen to have a manual (or even a scan of manual) that they could send me to brush up my addled memory. Bonus question: This college is working on lobster tissue. Does anyone have any suggestions for a processing schedule, or tips on how best to deal with these yummy bugs? Thanks ... again, Amos Brooks From WWmn916 <@t> aol.com Thu Feb 14 22:31:14 2008 From: WWmn916 <@t> aol.com (WWmn916@aol.com) Date: Thu Feb 14 22:31:29 2008 Subject: [Histonet] Food and drink? Message-ID: Dare I ask this question, Does anyone use closed topped and safety mugs at their microtome station when cutting slides...etc.? Would cutting paraffin blocks constitute Blood Borne Pathogen concerns? I fully understand not eating while paraffin is flying all over the place......but closed containers with coffee for those of us who work sleep deprived hours? Someone save us! Anonymous **************The year's hottest artists on the red carpet at the Grammy Awards. Go to AOL Music. (http://music.aol.com/grammys?NCID=aolcmp00300000002565) From caneves <@t> ufv.br Fri Feb 15 05:44:41 2008 From: caneves <@t> ufv.br (Clovis A Neves) Date: Fri Feb 15 05:44:54 2008 Subject: [Histonet] Labelling Macrophage whitout antibody Message-ID: <000001c86fc8$26f74220$74e5c660$@br> Somebody knows some technique to identify macrophage in Historesin (Leica) embedded tissue? Thanks in advance Clovis Neves Brazil From kmerriam2003 <@t> yahoo.com Fri Feb 15 06:21:28 2008 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Fri Feb 15 06:21:32 2008 Subject: [Histonet] Fwd: [IHCRG] Re: zebrafish Message-ID: <273862.10590.qm@web50310.mail.re2.yahoo.com> Note: forwarded message attached. Kim Merriam, MA, HT(ASCP) Cambridge, MA --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From rjbuesa <@t> yahoo.com Fri Feb 15 07:13:04 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 15 07:13:08 2008 Subject: [Histonet] Lobster Processing on VIP 2000 In-Reply-To: <582736990802141842o131e51edha8ff329fb7193541@mail.gmail.com> Message-ID: <554252.95983.qm@web61223.mail.yahoo.com> If for lobster you are referring to the North clawed ones (Homarus spp.) they can be processed with a standard protocol, but if you are referring to the the spiny lobsters (Panulirus spp.) you will have to be careful with dehydration, since their tissues, specially the gonads, dehydrate very quickly. You should use a very gentle and graded dehydration, and as short as possible. Ren? J. Amos Brooks wrote: Bonus question: This college is working on lobster tissue. Does anyone have any suggestions for a processing schedule, or tips on how best to deal with these yummy bugs? Thanks ... again, Amos Brooks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From rjbuesa <@t> yahoo.com Fri Feb 15 07:18:09 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 15 07:18:14 2008 Subject: [Histonet] Food and drink? In-Reply-To: Message-ID: <375365.78946.qm@web61222.mail.yahoo.com> For your information: smoking, drinking and eating, applying cosmetics, or even using contact lens are ABSOLUTELY prohibited in a medical histology laboratory setting. Should I imply by your question that you do not work in a medical lab. setting? Some universities and animal labs have been able to get away with ignoring those regulations up to now, but not any medical lab. that even have to have designated lounge areas. Ren? J. WWmn916@aol.com wrote: Dare I ask this question, Does anyone use closed topped and safety mugs at their microtome station when cutting slides...etc.? Would cutting paraffin blocks constitute Blood Borne Pathogen concerns? I fully understand not eating while paraffin is flying all over the place......but closed containers with coffee for those of us who work sleep deprived hours? Someone save us! Anonymous **************The year's hottest artists on the red carpet at the Grammy Awards. Go to AOL Music. (http://music.aol.com/grammys?NCID=aolcmp00300000002565) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From GauchV <@t> mail.amc.edu Fri Feb 15 07:36:37 2008 From: GauchV <@t> mail.amc.edu (Vicki Gauch) Date: Fri Feb 15 07:37:45 2008 Subject: [Histonet] Food and drink? Message-ID: Dear Anonymous, We are not allowed to have any food or drink in the laboratory- even if it is covered. Our inspectors have looked for this in the past so we are pretty strict about that here. Vicki AMCH Albany, NY >>> 2/14/2008 11:31 PM >>> Dare I ask this question, Does anyone use closed topped and safety mugs at their microtome station when cutting slides...etc.? Would cutting paraffin blocks constitute Blood Borne Pathogen concerns? I fully understand not eating while paraffin is flying all over the place......but closed containers with coffee for those of us who work sleep deprived hours? Someone save us! Anonymous **************The year's hottest artists on the red carpet at the Grammy Awards. Go to AOL Music. (http://music.aol.com/grammys?NCID=aolcmp00300000002565) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. From Terry.Marshall <@t> rothgen.nhs.uk Fri Feb 15 07:51:40 2008 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Feb 15 07:51:26 2008 Subject: [Histonet] Food and drink? Message-ID: <5C0BED61F529364E86309CADEA63FEF20163F2EE@TRFT-EX01.xRothGen.nhs.uk> Using contact lenses forbidden!!! You can't be serious. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 15 February 2008 13:18 To: WWmn916@aol.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Food and drink? For your information: smoking, drinking and eating, applying cosmetics, or even using contact lens are ABSOLUTELY prohibited in a medical histology laboratory setting. Should I imply by your question that you do not work in a medical lab. setting? Some universities and animal labs have been able to get away with ignoring those regulations up to now, but not any medical lab. that even have to have designated lounge areas. Ren? J. WWmn916@aol.com wrote: Dare I ask this question, Does anyone use closed topped and safety mugs at their microtome station when cutting slides...etc.? Would cutting paraffin blocks constitute Blood Borne Pathogen concerns? I fully understand not eating while paraffin is flying all over the place......but closed containers with coffee for those of us who work sleep deprived hours? Someone save us! Anonymous **************The year's hottest artists on the red carpet at the Grammy Awards. Go to AOL Music. (http://music.aol.com/grammys?NCID=aolcmp00300000002565) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Fri Feb 15 07:58:17 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Feb 15 07:58:20 2008 Subject: [Histonet] Lac Z gene and GFP In-Reply-To: <5.2.1.1.2.20080214131220.00ab4fb0@postoffice6.mail.cornell.edu> References: <5.2.1.1.2.20080214131220.00ab4fb0@postoffice6.mail.cornell.edu> Message-ID: For GFP, we use an antibody from Invitrogen--they have goat, mouse, and rabbit, at least. Our secondary is Cy2 from Jackson Immuno. You could try to dissect the muscle to see the GFP without an antibody, but it usually bleaches due to exposure to light. Emiliy -- fortune smiles on the brave and spits on the coward --aguirre, wrath of god From vazquezr <@t> ohsu.edu Fri Feb 15 08:25:52 2008 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Feb 15 08:26:24 2008 Subject: [Histonet] Food and drink? Message-ID: Rene, There goes my mid-morning martini...promise to put a petri dish on the top of it....:D It's Friday!!! Robyn >>> "Rene J Buesa" 2/15/2008 5:18 AM >>> For your information: smoking, drinking and eating, applying cosmetics, or even using contact lens are ABSOLUTELY prohibited in a medical histology laboratory setting. Should I imply by your question that you do not work in a medical lab. setting? Some universities and animal labs have been able to get away with ignoring those regulations up to now, but not any medical lab. that even have to have designated lounge areas. Ren? J. WWmn916@aol.com wrote: Dare I ask this question, Does anyone use closed topped and safety mugs at their microtome station when cutting slides...etc.? Would cutting paraffin blocks constitute Blood Borne Pathogen concerns? I fully understand not eating while paraffin is flying all over the place......but closed containers with coffee for those of us who work sleep deprived hours? Someone save us! Anonymous **************The year's hottest artists on the red carpet at the Grammy Awards. Go to AOL Music. (http://music.aol.com/grammys?NCID=aolcmp00300000002565) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bakevictoria <@t> gmail.com Fri Feb 15 08:43:28 2008 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Fri Feb 15 08:43:33 2008 Subject: [Histonet] Food and drink? In-Reply-To: References: Message-ID: <4f016b690802150643o52374455p92516d7aea6be727@mail.gmail.com> Here I go - in for a penny; in for a pound! I'm going to be honest and say that I have worked in labs (recently) where - yes the staff will keep covered coffee/tea/water bottles by their cutting areas. Supervisors do NOT condone this practice and if seen the tech risks a write up or other disciplinary action. As a supervisor I used to let the staff keep their coffee/tea in my office so that they could slip in and grab a sip. This worked until one day a surgeon came into the gross room with a cup of coffee and when asked to take it out of the room essentially told me to go to hell and how to get there. After that my staff had a difficult time with the "rule" as they called it. There is a reason for these rules and you need to think about why it's there. It may seem like over-kill, but would you want to risk it? As to Terry's response to the contact lenses I can personally attest to the 'no contact lens' rule. Back in the early 80's (when I was young and dumb) I was a wearer of soft contact lenses, one day the lenses turned cloudy and dried up on my eyes. It was a very scary event. Lenses need moisture and in the lab all the hydrocarbons (xylene, formaldehyde etc) take moisture out of the air causing the lenses to just dry out. I can't even wear contacts anymore as a result. Vikki On 2/14/08, WWmn916@aol.com wrote: > Dare I ask this question, > Does anyone use closed topped and safety mugs at their microtome station when > cutting slides...etc.? Would cutting paraffin blocks constitute Blood Borne > Pathogen concerns? I fully understand not eating while paraffin is flying all > over the place......but closed containers with coffee for those of us who > work sleep deprived hours? Someone save us! > > Anonymous > > > > **************The year's hottest artists on the red carpet at the Grammy > Awards. Go to AOL Music. > (http://music.aol.com/grammys?NCID=aolcmp00300000002565) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mpence <@t> grhs.net Fri Feb 15 08:43:49 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Feb 15 08:43:54 2008 Subject: [Histonet] PAS w/wout Diastase liver controls In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A371A@IS-E2K3.grhs.net> You cannot precut controls of liver for most special and IHC stains for more than a week at a time without affecting the stain quality. I learned this the hard way! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Caldwell, Lia Sent: Wednesday, February 13, 2008 6:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAS w/wout Diastase liver controls Hey all, Just wondering if I could pick your brains about the inconsistency of the PAS with and without Diastase method on liver controls. We cut several unstained slides on our liver patient panels, and if they happen to have a positive reaction, we will keep the unstained slides to use as future controls. We also have a liver control that was once very good for this method. We have noticed that, after time, the PAS reaction weakens on the PAS w/out D on both controls. Our results are not consistent regardless if you use saliva or Diastase of Malt & buffer. Sometimes the patient's stain is better than the control that was beautiful weeks before. Can this be attributed to the continual breakdown of glycogen in the liver even after processing? Does anyone have solid research to indicate tissue/substance degeneration long after processing? Any information would help. Thank you! ~Lia Lia M. Caldwell HT (ASCP) Histology Supervisor Oro Valley Pathology Dept. phone: (520) 901-3914 www.Lia.Caldwell@TriadHospitals.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> heart.thi.tmc.edu Fri Feb 15 08:48:12 2008 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Feb 15 08:48:15 2008 Subject: [Histonet] PTAH on frozen sections Message-ID: Happy Friday! Is there any change I should make in my PTAH protocol when staining frozen sections? Thanks! Betsy Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 From rjbuesa <@t> yahoo.com Fri Feb 15 08:50:16 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 15 08:50:21 2008 Subject: [Histonet] Food and drink? In-Reply-To: <5C0BED61F529364E86309CADEA63FEF20163F2EE@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <50049.67753.qm@web61223.mail.yahoo.com> Tell that to those writing the regulations. The rationale being that fumes could be trapped between the cornea and the contact lens causing irritations and potentially damaging the cornea. Ren? J. "Marshall Terry Dr, Consultant Histopathologist" wrote: Using contact lenses forbidden!!! You can't be serious. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 15 February 2008 13:18 To: WWmn916@aol.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Food and drink? For your information: smoking, drinking and eating, applying cosmetics, or even using contact lens are ABSOLUTELY prohibited in a medical histology laboratory setting. Should I imply by your question that you do not work in a medical lab. setting? Some universities and animal labs have been able to get away with ignoring those regulations up to now, but not any medical lab. that even have to have designated lounge areas. Ren? J. WWmn916@aol.com wrote: Dare I ask this question, Does anyone use closed topped and safety mugs at their microtome station when cutting slides...etc.? Would cutting paraffin blocks constitute Blood Borne Pathogen concerns? I fully understand not eating while paraffin is flying all over the place......but closed containers with coffee for those of us who work sleep deprived hours? Someone save us! Anonymous **************The year's hottest artists on the red carpet at the Grammy Awards. Go to AOL Music. (http://music.aol.com/grammys?NCID=aolcmp00300000002565) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From rjbuesa <@t> yahoo.com Fri Feb 15 08:57:11 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 15 08:57:16 2008 Subject: [Histonet] Food and drink? In-Reply-To: Message-ID: <720802.42886.qm@web61221.mail.yahoo.com> Robyn: Was it "shaken" or "stirred"? Mind the olive though! Ren? J. Robyn Vazquez wrote: Rene, There goes my mid-morning martini...promise to put a petri dish on the top of it....:D It's Friday!!! Robyn >>> "Rene J Buesa" 2/15/2008 5:18 AM >>> For your information: smoking, drinking and eating, applying cosmetics, or even using contact lens are ABSOLUTELY prohibited in a medical histology laboratory setting. Should I imply by your question that you do not work in a medical lab. setting? Some universities and animal labs have been able to get away with ignoring those regulations up to now, but not any medical lab. that even have to have designated lounge areas. Ren? J. WWmn916@aol.com wrote: Dare I ask this question, Does anyone use closed topped and safety mugs at their microtome station when cutting slides...etc.? Would cutting paraffin blocks constitute Blood Borne Pathogen concerns? I fully understand not eating while paraffin is flying all over the place......but closed containers with coffee for those of us who work sleep deprived hours? Someone save us! Anonymous **************The year's hottest artists on the red carpet at the Grammy Awards. Go to AOL Music. (http://music.aol.com/grammys?NCID=aolcmp00300000002565) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From rjbuesa <@t> yahoo.com Fri Feb 15 09:03:11 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 15 09:03:14 2008 Subject: [Histonet] Food and drink? In-Reply-To: <4f016b690802150643o52374455p92516d7aea6be727@mail.gmail.com> Message-ID: <339633.57257.qm@web61224.mail.yahoo.com> Victoria: Your annecdote with the surgeon, reminds me a similar case. The surgeon in my case, as almost all of them, used "to confer with God every other Thursday". I spoke with the hospital director, the surgeon got a written reprimand, never ever broke the rule again, neither any of the members of my staff. Ren? J. Victoria Baker wrote: --------------------------------- Never miss a thing. Make Yahoo your homepage. From jqb7 <@t> CDC.GOV Fri Feb 15 09:07:49 2008 From: jqb7 <@t> CDC.GOV (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri Feb 15 09:12:49 2008 Subject: [Histonet] Food and drink? In-Reply-To: <50049.67753.qm@web61223.mail.yahoo.com> References: <5C0BED61F529364E86309CADEA63FEF20163F2EE@TRFT-EX01.xRothGen.nhs.uk> <50049.67753.qm@web61223.mail.yahoo.com> Message-ID: <34BB307EFC9A65429BBB49E330675F72045E256D@LTA3VS003.ees.hhs.gov> Also, I have seen people remove and reinsert their contacts while in the lab. Definitely not a good idea. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, February 15, 2008 9:50 AM To: Marshall Terry Dr, Consultant Histopathologist; WWmn916@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Food and drink? Tell that to those writing the regulations. The rationale being that fumes could be trapped between the cornea and the contact lens causing irritations and potentially damaging the cornea. Ren? J. "Marshall Terry Dr, Consultant Histopathologist" wrote: Using contact lenses forbidden!!! You can't be serious. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 15 February 2008 13:18 To: WWmn916@aol.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Food and drink? For your information: smoking, drinking and eating, applying cosmetics, or even using contact lens are ABSOLUTELY prohibited in a medical histology laboratory setting. Should I imply by your question that you do not work in a medical lab. setting? Some universities and animal labs have been able to get away with ignoring those regulations up to now, but not any medical lab. that even have to have designated lounge areas. Ren? J. WWmn916@aol.com wrote: Dare I ask this question, Does anyone use closed topped and safety mugs at their microtome station when cutting slides...etc.? Would cutting paraffin blocks constitute Blood Borne Pathogen concerns? I fully understand not eating while paraffin is flying all over the place......but closed containers with coffee for those of us who work sleep deprived hours? Someone save us! Anonymous **************The year's hottest artists on the red carpet at the Grammy Awards. Go to AOL Music. (http://music.aol.com/grammys?NCID=aolcmp00300000002565) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dellav <@t> musc.edu Fri Feb 15 09:23:10 2008 From: dellav <@t> musc.edu (Della Speranza, Vinnie) Date: Fri Feb 15 09:22:37 2008 Subject: [Histonet] PAS + IF In-Reply-To: <582736990802141836u25754898n733d083325354a31@mail.gmail.com> References: <582736990802141836u25754898n733d083325354a31@mail.gmail.com> Message-ID: Amos, do you or the researcher know that basic fuchsin or its constituent dyes (rosanilin, pararosaniline) will not fluoresce with the incident excitation wavelength that will be used for the fluorescent tag? Aside from the issues you raised this could make for interpretation difficulties Vinnie Della Speranza Manager for Anatomic Pathology Services 165 Ashley Avenue Suite 309 Charleston, South Carolina 29425 Tel: (843) 792-6353 Fax: (843) 792-8974 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Thursday, February 14, 2008 9:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAS + IF Hi, There is a researcher here that is interested in doing a PAS and an immunofluorescent label on the same slide. She is using formalin fixed paraffin embedded tissue and the antibody requires heat induced epitope retrieval. So obviously the PAS will need to come first as the reagents involved would innevitably destroy the fluorescent label. The question is: will the retrieval affect the PAS reaction? My initial guess was that the PAS is a fairly strong bond (spill schiffs on your hand ... see what I mean!) so it should survive, but that is just a guess. Has anyone tried it? Thanks, Amos Brooks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From barbara.verstraeten <@t> ugent.be Fri Feb 15 09:22:38 2008 From: barbara.verstraeten <@t> ugent.be (Barbara Verstraeten) Date: Fri Feb 15 09:22:44 2008 Subject: [Histonet] Technovit 9100 problems Message-ID: <20080215162238.c1out4lxq80kwkoo@webmail.ugent.be> Dear all, My name is Barbara Verstraeten and I'm a first yeat phD student at the university of Ghent, Belgium. I work on very small zebrafish (40 hours post fertilisation 'till 6 days post fertilisation). I want to do immunostaining on them so I embedded them in technovit 9100. Everything worked fine but now I have to cut them and put them on slides. My questions: * What sort of knife do I use? * I tried to cut them at 3 ?m, but they roll up or fall apart. Hints? * I put my sections on super frost slides. Here we coat them with poly-L-lysine. Does anyone have other suggestions on coating slides so that de sections will stick very good? Thank you very much! Barbara Verstraeten, phD Lab Ann Huysseune Ghent, Belgium mail:barbara.verstraeten@ugent.be From funderwood <@t> mcohio.org Fri Feb 15 09:28:18 2008 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Fri Feb 15 09:31:31 2008 Subject: [Histonet] Food and drink? In-Reply-To: <720802.42886.qm@web61221.mail.yahoo.com> References: <720802.42886.qm@web61221.mail.yahoo.com> Message-ID: <47B56A64.BE64.0034.0@mcohio.org> Is a tissue flotation bath/crock pot a bad idea? >>> Rene J Buesa 2/15/2008 9:57 AM >>> Robyn: Was it "shaken" or "stirred"? Mind the olive though! Ren? J. Robyn Vazquez wrote: Rene, There goes my mid-morning martini...promise to put a petri dish on the top of it....:D It's Friday!!! Robyn >>> "Rene J Buesa" 2/15/2008 5:18 AM >>> For your information: smoking, drinking and eating, applying cosmetics, or even using contact lens are ABSOLUTELY prohibited in a medical histology laboratory setting. Should I imply by your question that you do not work in a medical lab. setting? Some universities and animal labs have been able to get away with ignoring those regulations up to now, but not any medical lab. that even have to have designated lounge areas. Ren? J. WWmn916@aol.com wrote: Dare I ask this question, Does anyone use closed topped and safety mugs at their microtome station when cutting slides...etc.? Would cutting paraffin blocks constitute Blood Borne Pathogen concerns? I fully understand not eating while paraffin is flying all over the place......but closed containers with coffee for those of us who work sleep deprived hours? Someone save us! Anonymous **************The year's hottest artists on the red carpet at the Grammy Awards. Go to AOL Music. (http://music.aol.com/grammys?NCID=aolcmp00300000002565) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bakevictoria <@t> gmail.com Fri Feb 15 09:34:15 2008 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Fri Feb 15 09:34:23 2008 Subject: [Histonet] Food and drink? In-Reply-To: <47B56A64.BE64.0034.0@mcohio.org> References: <720802.42886.qm@web61221.mail.yahoo.com> <47B56A64.BE64.0034.0@mcohio.org> Message-ID: <4f016b690802150734p41afa147l55ed77e05eb224ba@mail.gmail.com> boiled pancreas or brisket? I'm laughing too hard! On 2/15/08, Fred Underwood wrote: > Is a tissue flotation bath/crock pot a bad idea? > > >>> Rene J Buesa 2/15/2008 9:57 AM >>> > Robyn: > Was it "shaken" or "stirred"? Mind the olive though! > Ren? J. > > Robyn Vazquez wrote: Rene, > There goes my mid-morning martini...promise to put a petri dish on the top of it....:D It's Friday!!! > > Robyn > > > >>> "Rene J Buesa" 2/15/2008 5:18 AM >>> > > For your information: smoking, drinking and eating, applying cosmetics, or even using contact lens are ABSOLUTELY prohibited in a medical histology laboratory setting. > Should I imply by your question that you do not work in a medical lab. setting? Some universities and animal labs have been able to get away with ignoring those regulations up to now, but not any medical lab. that even have to have designated lounge areas. > Ren? J. > > > WWmn916@aol.com wrote: > Dare I ask this question, > Does anyone use closed topped and safety mugs at their microtome station when > cutting slides...etc.? Would cutting paraffin blocks constitute Blood Borne > Pathogen concerns? I fully understand not eating while paraffin is flying all > over the place......but closed containers with coffee for those of us who > work sleep deprived hours? Someone save us! > > Anonymous > > > > **************The year's hottest artists on the red carpet at the Grammy > Awards. Go to AOL Music. > (http://music.aol.com/grammys?NCID=aolcmp00300000002565) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From TMcNemar <@t> lmhealth.org Fri Feb 15 09:43:31 2008 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Feb 15 09:43:20 2008 Subject: [Histonet] Food and drink? In-Reply-To: <5C0BED61F529364E86309CADEA63FEF20163F2EE@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F537@lmhsmail.lmhealth.org> We have the same rules as those below. As far as contacts, you can wear them, just not put them in or take them out at the bench. Don't know why you would, but that's what the rule says. I (probably like many), remember the days when it was perfectly acceptable to eat, drink, and smoke at the microtome. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Marshall Terry Dr, Consultant Histopathologist Sent: Friday, February 15, 2008 8:52 AM To: Rene J Buesa; WWmn916@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Food and drink? Using contact lenses forbidden!!! You can't be serious. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 15 February 2008 13:18 To: WWmn916@aol.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Food and drink? For your information: smoking, drinking and eating, applying cosmetics, or even using contact lens are ABSOLUTELY prohibited in a medical histology laboratory setting. Should I imply by your question that you do not work in a medical lab. setting? Some universities and animal labs have been able to get away with ignoring those regulations up to now, but not any medical lab. that even have to have designated lounge areas. Ren? J. WWmn916@aol.com wrote: Dare I ask this question, Does anyone use closed topped and safety mugs at their microtome station when cutting slides...etc.? Would cutting paraffin blocks constitute Blood Borne Pathogen concerns? I fully understand not eating while paraffin is flying all over the place......but closed containers with coffee for those of us who work sleep deprived hours? Someone save us! Anonymous **************The year's hottest artists on the red carpet at the Grammy Awards. Go to AOL Music. (http://music.aol.com/grammys?NCID=aolcmp00300000002565) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Fri Feb 15 09:47:12 2008 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Feb 15 09:48:09 2008 Subject: [Histonet] Food and drink? References: <720802.42886.qm@web61221.mail.yahoo.com> <47B56A64.BE64.0034.0@mcohio.org> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2508@fhosxchmb006.ADVENTISTCORP.NET> I've got a great meat-slicer!!!!! It even uses disposable blades...... ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Fred Underwood Sent: Fri 2/15/2008 10:28 AM To: WWmn916@aol.com; histonet@lists.utsouthwestern.edu; Robyn Vazquez; Rene J Buesa Subject: Re: [Histonet] Food and drink? Is a tissue flotation bath/crock pot a bad idea? >>> Rene J Buesa 2/15/2008 9:57 AM >>> Robyn: Was it "shaken" or "stirred"? Mind the olive though! Ren? J. Robyn Vazquez wrote: Rene, There goes my mid-morning martini...promise to put a petri dish on the top of it....:D It's Friday!!! Robyn >>> "Rene J Buesa" 2/15/2008 5:18 AM >>> For your information: smoking, drinking and eating, applying cosmetics, or even using contact lens are ABSOLUTELY prohibited in a medical histology laboratory setting. Should I imply by your question that you do not work in a medical lab. setting? Some universities and animal labs have been able to get away with ignoring those regulations up to now, but not any medical lab. that even have to have designated lounge areas. Ren? J. WWmn916@aol.com wrote: Dare I ask this question, Does anyone use closed topped and safety mugs at their microtome station when cutting slides...etc.? Would cutting paraffin blocks constitute Blood Borne Pathogen concerns? I fully understand not eating while paraffin is flying all over the place......but closed containers with coffee for those of us who work sleep deprived hours? Someone save us! Anonymous **************The year's hottest artists on the red carpet at the Grammy Awards. Go to AOL Music. (http://music.aol.com/grammys?NCID=aolcmp00300000002565) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From rjbuesa <@t> yahoo.com Fri Feb 15 09:57:32 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 15 09:57:35 2008 Subject: [Histonet] Technovit 9100 problems In-Reply-To: <20080215162238.c1out4lxq80kwkoo@webmail.ugent.be> Message-ID: <404296.86441.qm@web61221.mail.yahoo.com> Barbara: You are describing a problem that is common when small subjects (and in your case also with high water contents) have been dehydrated too quickly and probably "cleared" too long resulting in a brittle subject difficult to section and to be kept on the slides. Remedies after the fact are abundant and usually useless. I advise you to check your processing protocol and optimize it to your study subjects. I am sure that you have plenty available to make some tests and get to an adequate protocol. Once you do that, you will be fine and will not need advises like the one you are seeking now. Ren? J. Barbara Verstraeten wrote: --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From b-frederick <@t> northwestern.edu Fri Feb 15 10:14:10 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Feb 15 10:15:23 2008 Subject: [Histonet] Food and drink? In-Reply-To: <4f016b690802150734p41afa147l55ed77e05eb224ba@mail.gmail.com> Message-ID: <000401c86fed$d04bf3f0$d00f7ca5@lurie.northwestern.edu> Personally, I have one of the Fisher tissue prep waterbaths and it would make a lovely lasagna pan.......... do we want meat or salmon? Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Friday, February 15, 2008 9:34 AM To: Fred Underwood Cc: histonet@lists.utsouthwestern.edu; WWmn916@aol.com Subject: Re: [Histonet] Food and drink? boiled pancreas or brisket? I'm laughing too hard! On 2/15/08, Fred Underwood wrote: > Is a tissue flotation bath/crock pot a bad idea? > > >>> Rene J Buesa 2/15/2008 9:57 AM >>> > Robyn: > Was it "shaken" or "stirred"? Mind the olive though! > Ren? J. > > Robyn Vazquez wrote: Rene, > There goes my mid-morning martini...promise to put a petri dish on the top of it....:D It's Friday!!! > > Robyn > > > >>> "Rene J Buesa" 2/15/2008 5:18 AM >>> > > For your information: smoking, drinking and eating, applying cosmetics, or even using contact lens are ABSOLUTELY prohibited in a medical histology laboratory setting. > Should I imply by your question that you do not work in a medical lab. setting? Some universities and animal labs have been able to get away with ignoring those regulations up to now, but not any medical lab. that even have to have designated lounge areas. > Ren? J. > > > WWmn916@aol.com wrote: > Dare I ask this question, > Does anyone use closed topped and safety mugs at their microtome station when > cutting slides...etc.? Would cutting paraffin blocks constitute Blood Borne > Pathogen concerns? I fully understand not eating while paraffin is flying all > over the place......but closed containers with coffee for those of us who > work sleep deprived hours? Someone save us! > > Anonymous > > > > **************The year's hottest artists on the red carpet at the Grammy > Awards. Go to AOL Music. > (http://music.aol.com/grammys?NCID=aolcmp00300000002565) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Fri Feb 15 10:27:48 2008 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Feb 15 10:27:32 2008 Subject: [Histonet] Food and drink? Message-ID: <5C0BED61F529364E86309CADEA63FEF20163F2F1@TRFT-EX01.xRothGen.nhs.uk> Well, spoke too soon in my last post! That strikes me as overkill. We don't have that rule in the UK (AFAIK) It's also interesting inasmuch as when I wore contacts, up until a couple of years ago when I got dry eyes, I had no problem with peeling/cutting onions. Now they irritate my eyes and make them water like hell. (Odd that dry eyes water isn't it?) This suggests that they actually protect the eyes from fumes. Anyone else had this experience? Have a good weekend all. Terry ________________________________ From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: 15 February 2008 14:50 To: Marshall Terry Dr, Consultant Histopathologist; WWmn916@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Food and drink? Tell that to those writing the regulations. The rationale being that fumes could be trapped between the cornea and the contact lens causing irritations and potentially damaging the cornea. Ren? J. "Marshall Terry Dr, Consultant Histopathologist" wrote: Using contact lenses forbidden!!! You can't be serious. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 15 February 2008 13:18 To: WWmn916@aol.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Food and drink? For your information: smoking, drinking and eating, applying cosmetics, or even using contact lens are ABSOLUTELY prohibited in a medical histology laboratory setting. Should I imply by your question that you do not work in a medical lab. setting? Some universities and animal labs have been able to get away with ignoring those regulations up to now, but not any medical lab. that even have to have designated lounge areas. Ren? J. WWmn916@aol.com wrote: Dare I ask this question, Does anyone use closed topped and safety mugs at their microtome station when cutting slides...etc.? Would cutting paraffin blocks constitute Blood Borne Pathogen concerns? I fully understand not eating while paraffin is flying all over the place......but closed containers with coffee for those of us who work sleep deprived hours? Someone save us! Anonymous **************The year's hottest artists on the red carpet at the Grammy Awards. Go to AOL Music. (http://music.aol.com/grammys?NCID=aolcmp00300000002565) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Never miss a thing. Make Yahoo your homepage. From rjbuesa <@t> yahoo.com Fri Feb 15 10:52:39 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 15 10:52:42 2008 Subject: [Histonet] PTAH on frozen sections In-Reply-To: Message-ID: <192972.38560.qm@web61223.mail.yahoo.com> Just make absolutely sure that your sections are not going to fall. You could try to coat the slides with Mayer's albumin or with Elmer's glue. Let the slides with the coat dry in an oven overnight before using them. Put you FS on the coated slides and air dry them. Fix them afterwards in 10% NBF for 3-5 minutes and wash in water to eliminate the OCT and stain them afterwards with the PTAH. Do NOT use any microwave shortcut, but stain the sections in the PTAH overnight. Perhaps they will survive. Your problem will be with the sections adherence. Ren? J. "Molinari, Betsy" wrote: Happy Friday! Is there any change I should make in my PTAH protocol when staining frozen sections? Thanks! Betsy Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From lchung <@t> ppmh.org Fri Feb 15 10:53:08 2008 From: lchung <@t> ppmh.org (Chung, Luong) Date: Fri Feb 15 10:55:44 2008 Subject: [Histonet] Food and drink? In-Reply-To: <47B56A64.BE64.0034.0@mcohio.org> Message-ID: <86691924ECCDBE4F82CCAB55342460710195B4@exchange2.phoebe.com> In cytology we have a blender to process sputum and it will make a mean frozen margarita. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Fred Underwood Sent: Friday, February 15, 2008 10:28 AM To: WWmn916@aol.com; histonet@lists.utsouthwestern.edu; Robyn Vazquez; Rene J Buesa Subject: Re: [Histonet] Food and drink? Is a tissue flotation bath/crock pot a bad idea? >>> Rene J Buesa 2/15/2008 9:57 AM >>> Robyn: Was it "shaken" or "stirred"? Mind the olive though! Ren? J. Robyn Vazquez wrote: Rene, There goes my mid-morning martini...promise to put a petri dish on the top of it....:D It's Friday!!! Robyn >>> "Rene J Buesa" 2/15/2008 5:18 AM >>> For your information: smoking, drinking and eating, applying cosmetics, or even using contact lens are ABSOLUTELY prohibited in a medical histology laboratory setting. Should I imply by your question that you do not work in a medical lab. setting? Some universities and animal labs have been able to get away with ignoring those regulations up to now, but not any medical lab. that even have to have designated lounge areas. Ren? J. WWmn916@aol.com wrote: Dare I ask this question, Does anyone use closed topped and safety mugs at their microtome station when cutting slides...etc.? Would cutting paraffin blocks constitute Blood Borne Pathogen concerns? I fully understand not eating while paraffin is flying all over the place......but closed containers with coffee for those of us who work sleep deprived hours? Someone save us! Anonymous **************The year's hottest artists on the red carpet at the Grammy Awards. Go to AOL Music. (http://music.aol.com/grammys?NCID=aolcmp00300000002565) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Disclaimer: The HIPAA Final Privacy Rule requires covered entities to safeguard certain Protected Health Information (PHI) related to a person's healthcare. Information being faxed to you may include PHI after appropriate authorization from the patient or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain PHI in a safe and secure manner. You may not re-disclose without additional patient consent or as required by law. Unauthorized re-disclosure or failure to safeguard PHI could subject you to penalties described in federal (HIPAA) and state law. If you the reader of this message are not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, please notify us immediately and destroy the related message. From maa8 <@t> cornell.edu Fri Feb 15 11:47:32 2008 From: maa8 <@t> cornell.edu (Mary A. Ascenzi) Date: Fri Feb 15 11:48:52 2008 Subject: [Histonet] Food and drink? In-Reply-To: References: Message-ID: Nope, can't do it. I've seen some research labs that have small shelves outside the lab door where people park their covered coffee cups so they can run out for a quick gulp. Kind of reminds me of rats pressing on levers for food pellet rewards. Mary >Dare I ask this question, >Does anyone use closed topped and safety mugs at their microtome station when >cutting slides...etc.? Would cutting paraffin blocks constitute Blood Borne >Pathogen concerns? I fully understand not eating while paraffin is flying all >over the place......but closed containers with coffee for those of us who >work sleep deprived hours? Someone save us! > >Anonymous > > > >**************The year's hottest artists on the red carpet at the Grammy >Awards. Go to AOL Music. >(http://music.aol.com/grammys?NCID=aolcmp00300000002565) >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Fri Feb 15 11:56:18 2008 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Feb 15 11:56:38 2008 Subject: FW: [Histonet] Food and drink? References: <000401c86fed$d04bf3f0$d00f7ca5@lurie.northwestern.edu> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F250B@fhosxchmb006.ADVENTISTCORP.NET> And this was not an arbitrary rule.....several techs at a University in the USA contracted Hepatitis from drinking their coffee which became contaminated by particulate aerosol from the specimen being worked on... and three of them died. -True story. And the beginning of of this regulation. > >>> "Rene J Buesa" 2/15/2008 5:18 AM >>> > > For your information: smoking, drinking and eating, applying cosmetics, or even using contact lens are ABSOLUTELY prohibited in a medical histology laboratory setting. > Should I imply by your question that you do not work in a medical lab. setting? Some universities and animal labs have been able to get away with ignoring those regulations up to now, but not any medical lab. that even have to have designated lounge areas. > Ren? J. > > > WWmn916@aol.com wrote: > Dare I ask this question, > Does anyone use closed topped and safety mugs at their microtome station when > cutting slides...etc.? Would cutting paraffin blocks constitute Blood Borne > Pathogen concerns? I fully understand not eating while paraffin is flying all > over the place......but closed containers with coffee for those of us who > work sleep deprived hours? Someone save us! > > Anonymous > > > > **************The year's hottest artists on the red carpet at the Grammy > Awards. Go to AOL Music. > (http://music.aol.com/grammys?NCID=aolcmp00300000002565) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From juditw <@t> u.washington.edu Fri Feb 15 12:05:20 2008 From: juditw <@t> u.washington.edu (Judith L. Williams) Date: Fri Feb 15 12:05:24 2008 Subject: [Histonet] Food and drink? In-Reply-To: <4f016b690802150734p41afa147l55ed77e05eb224ba@mail.gmail.com> Message-ID: GOTTA LOVE THE FRIDAY - ITS TIME TO GET THE HEY OUT OF HERE HUMOR.......rock on humourous histotechs! Judy On Fri, 15 Feb 2008, Victoria Baker wrote: > boiled pancreas or brisket? I'm laughing too hard! > > On 2/15/08, Fred Underwood wrote: >> Is a tissue flotation bath/crock pot a bad idea? >> >>>>> Rene J Buesa 2/15/2008 9:57 AM >>> >> Robyn: >> Was it "shaken" or "stirred"? Mind the olive though! >> Ren? J. >> >> Robyn Vazquez wrote: Rene, >> There goes my mid-morning martini...promise to put a petri dish on the top of it....:D It's Friday!!! >> >> Robyn >> >> >>>>> "Rene J Buesa" 2/15/2008 5:18 AM >>> >> >> For your information: smoking, drinking and eating, applying cosmetics, or even using contact lens are ABSOLUTELY prohibited in a medical histology laboratory setting. >> Should I imply by your question that you do not work in a medical lab. setting? Some universities and animal labs have been able to get away with ignoring those regulations up to now, but not any medical lab. that even have to have designated lounge areas. >> Ren? J. >> >> >> WWmn916@aol.com wrote: >> Dare I ask this question, >> Does anyone use closed topped and safety mugs at their microtome station when >> cutting slides...etc.? Would cutting paraffin blocks constitute Blood Borne >> Pathogen concerns? I fully understand not eating while paraffin is flying all >> over the place......but closed containers with coffee for those of us who >> work sleep deprived hours? Someone save us! >> >> Anonymous >> >> >> >> **************The year's hottest artists on the red carpet at the Grammy >> Awards. Go to AOL Music. >> (http://music.aol.com/grammys?NCID=aolcmp00300000002565) >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> --------------------------------- >> Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> >> --------------------------------- >> Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From CIngles <@t> uwhealth.org Fri Feb 15 12:08:21 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Fri Feb 15 12:11:33 2008 Subject: [Histonet] Food and drink? References: <339633.57257.qm@web61224.mail.yahoo.com> Message-ID: <08A0A863637F1349BBFD83A96B27A50A1200EC@uwhis-xchng3.uwhis.hosp.wisc.edu> Yea, I was getting nowhere with my Doc walking through with his coffee, snacks, etc.(those of you who know me, know how fiesty I can get.) I finally had to get my supervisor to talk to him. That would have been great for a CLIA/JCAHO inspection. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Fri 2/15/2008 9:03 AM To: Victoria Baker; WWmn916@aol.com Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Food and drink? Victoria: Your annecdote with the surgeon, reminds me a similar case. The surgeon in my case, as almost all of them, used "to confer with God every other Thursday". I spoke with the hospital director, the surgeon got a written reprimand, never ever broke the rule again, neither any of the members of my staff. Ren? J. Victoria Baker wrote: --------------------------------- Never miss a thing. Make Yahoo your homepage. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From integrated.histo <@t> gmail.com Fri Feb 15 12:18:53 2008 From: integrated.histo <@t> gmail.com (Cindy DuBois) Date: Fri Feb 15 12:19:00 2008 Subject: [Histonet] Coffee Cup Message-ID: <5d9104a30802151018q477bb6f5u3005362ce51c5cd4@mail.gmail.com> We have closed top coffee cups while embedding, but not cutting. P.S. Don't tell the safety compliance officer Anonymous #2 From micro <@t> superlink.net Fri Feb 15 12:29:36 2008 From: micro <@t> superlink.net (Markus F. Meyenhofer) Date: Fri Feb 15 12:30:22 2008 Subject: [Histonet] Food and drink? References: <339633.57257.qm@web61224.mail.yahoo.com> <08A0A863637F1349BBFD83A96B27A50A1200EC@uwhis-xchng3.uwhis.hosp.wisc.edu> Message-ID: <015e01c87000$bf36a700$8c893cd1@DJ4VDH31> The next GREAT Idea for the "lab police": Hazmat suits for histotechs!!! ----- Original Message ----- From: "Ingles Claire" Cc: Sent: Friday, February 15, 2008 1:08 PM Subject: RE: [Histonet] Food and drink? Yea, I was getting nowhere with my Doc walking through with his coffee, snacks, etc.(those of you who know me, know how fiesty I can get.) I finally had to get my supervisor to talk to him. That would have been great for a CLIA/JCAHO inspection. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Fri 2/15/2008 9:03 AM To: Victoria Baker; WWmn916@aol.com Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Food and drink? Victoria: Your annecdote with the surgeon, reminds me a similar case. The surgeon in my case, as almost all of them, used "to confer with God every other Thursday". I spoke with the hospital director, the surgeon got a written reprimand, never ever broke the rule again, neither any of the members of my staff. Ren? J. Victoria Baker wrote: --------------------------------- Never miss a thing. Make Yahoo your homepage. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynne.Bell <@t> hitchcock.org Fri Feb 15 12:37:01 2008 From: Lynne.Bell <@t> hitchcock.org (Bell, Lynne) Date: Fri Feb 15 12:37:08 2008 Subject: [Histonet] Food and drink? In-Reply-To: <5C0BED61F529364E86309CADEA63FEF20163F2F1@TRFT-EX01.xRothGen.nhs.uk> Message-ID: I wear contact lenses in the lab, but I also wear safety glasses all the time. I have been wearing lenses in histology for over thirty years and have never had a problem. And yes, Terry, when I cut onions while wearing contacts, my eyes do not water. I believe they do protect your eyes!! Lynne From oshel1pe <@t> cmich.edu Fri Feb 15 12:39:38 2008 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Fri Feb 15 12:39:49 2008 Subject: [Histonet] Food and drink? Message-ID: I had to remodel an unused darkroom into a break room to keep students and faculty from carrying food & drink into the lab. Worked, though. But ... with all the coffee fanatics on the list, we need a new brand: HistoBrew "Coffee so thick you can cut it." Phil -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576 From timothy.macatee <@t> med.nyu.edu Fri Feb 15 12:45:46 2008 From: timothy.macatee <@t> med.nyu.edu (Timothy Macatee) Date: Fri Feb 15 12:48:28 2008 Subject: [Histonet] Food and drink? In-Reply-To: Message-ID: If you want your lunch meat cut thin, where else are you going to go? Tim On 2/15/08 1:05 PM, "Judith L. Williams" wrote: > GOTTA LOVE THE FRIDAY - ITS TIME TO GET THE HEY OUT OF HERE HUMOR.......rock > on humourous histotechs! > Judy > > > On Fri, 15 Feb 2008, Victoria Baker wrote: > >> boiled pancreas or brisket? I'm laughing too hard! >> >> On 2/15/08, Fred Underwood wrote: >>> Is a tissue flotation bath/crock pot a bad idea? >>> >>>>>> Rene J Buesa 2/15/2008 9:57 AM >>> >>> Robyn: >>> Was it "shaken" or "stirred"? Mind the olive though! >>> Ren? J. >>> >>> Robyn Vazquez wrote: Rene, >>> There goes my mid-morning martini...promise to put a petri dish on the top >>> of it....:D It's Friday!!! >>> >>> Robyn >>> >>> >>>>>> "Rene J Buesa" 2/15/2008 5:18 AM >>> >>> >>> For your information: smoking, drinking and eating, applying cosmetics, or >>> even using contact lens are ABSOLUTELY prohibited in a medical histology >>> laboratory setting. >>> Should I imply by your question that you do not work in a medical lab. >>> setting? Some universities and animal labs have been able to get away with >>> ignoring those regulations up to now, but not any medical lab. that even >>> have to have designated lounge areas. >>> Ren? J. >>> >>> >>> WWmn916@aol.com wrote: >>> Dare I ask this question, >>> Does anyone use closed topped and safety mugs at their microtome station >>> when >>> cutting slides...etc.? Would cutting paraffin blocks constitute Blood Borne >>> Pathogen concerns? I fully understand not eating while paraffin is flying >>> all >>> over the place......but closed containers with coffee for those of us who >>> work sleep deprived hours? Someone save us! >>> >>> Anonymous >>> >>> >>> >>> **************The year's hottest artists on the red carpet at the Grammy >>> Awards. Go to AOL Music. >>> (http://music.aol.com/grammys?NCID=aolcmp00300000002565) >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >>> >>> --------------------------------- >>> Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it >>> now. >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >>> >>> >>> >>> --------------------------------- >>> Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it >>> now. >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Tim Macatee Research Histology Core New York University School of Medicine 550 First Ave. Department of Pathology. Medical Science Building - Room 504 New York, N.Y. 10016 (212) 263-3888 From trinimaican2501 <@t> yahoo.com Fri Feb 15 13:16:18 2008 From: trinimaican2501 <@t> yahoo.com (I-sanna Gibbons) Date: Fri Feb 15 13:16:21 2008 Subject: [Histonet] snail: shell and all Message-ID: <560994.70068.qm@web50309.mail.re2.yahoo.com> Hi all! Thanks so much for all the advice. I sent the information to the technician. I'll let you know what he ends up trying. Thanks again I-sanna Gibbons DVM ____________________________________________________________________________________ Looking for last minute shopping deals? Find them fast with Yahoo! Search. http://tools.search.yahoo.com/newsearch/category.php?category=shopping From alonso.martinezcanabal <@t> utoronto.ca Fri Feb 15 13:24:32 2008 From: alonso.martinezcanabal <@t> utoronto.ca (Alonso Martinez-Canabal) Date: Fri Feb 15 13:26:26 2008 Subject: [Histonet] Caspase-3-problem In-Reply-To: References: <5C0BED61F529364E86309CADEA63FEF20163F2F1@TRFT-EX01.xRothGen.nhs.uk> Message-ID: <60F7DC6E18EE4530BF33A4FFDFD1648F@Astrocito> Hi everyone, I have been trying to obtain a nice caspase-3-cleaved immunohistochemistry or immunofluorescence with frozen or vibratome sliced brains (particularly hippocampus). I have been using the cell signalling antibody, without a lot of success. I tried antigen retrieval with heating, and combined heating with proteinase K digestion, with not good results. Could you suggest me something? I also would like to know of any control tissue, I know that in normal young mouse brain should be some positive cells in the dentate gyrus, but do you kno any other structure that could be usable? Like skin, or liver, or anything like that? Thank you very much for your valuable help Alonso -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bell, Lynne Sent: February-15-08 1:37 PM To: Marshall Terry Dr, Consultant Histopathologist; Rene J Buesa; WWmn916@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Food and drink? I wear contact lenses in the lab, but I also wear safety glasses all the time. I have been wearing lenses in histology for over thirty years and have never had a problem. And yes, Terry, when I cut onions while wearing contacts, my eyes do not water. I believe they do protect your eyes!! Lynne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mwich <@t> 7thwavelabs.com Fri Feb 15 13:27:31 2008 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Fri Feb 15 13:27:38 2008 Subject: [Histonet] IHC in GMA media Message-ID: <2264717ADC396742A0FF0AAB674F9A0D486BAD@7THWAVE-SERVER.7thwave.local> Can anyone direct me to a website that contains info about IHC staining on plastic embedded sections? This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From JWEEMS <@t> sjha.org Fri Feb 15 13:37:16 2008 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Feb 15 13:37:26 2008 Subject: [Histonet] Food and drink? In-Reply-To: References: Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD320518E6A1@sjhaexc02.sjha.org> Only if we want to loose our license and our jobs.... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WWmn916@aol.com Sent: Thursday, February 14, 2008 11:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Food and drink? Dare I ask this question, Does anyone use closed topped and safety mugs at their microtome station when cutting slides...etc.? Would cutting paraffin blocks constitute Blood Borne Pathogen concerns? I fully understand not eating while paraffin is flying all over the place......but closed containers with coffee for those of us who work sleep deprived hours? Someone save us! Anonymous **************The year's hottest artists on the red carpet at the Grammy Awards. Go to AOL Music. (http://music.aol.com/grammys?NCID=aolcmp00300000002565) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From bill501 <@t> mindspring.com Fri Feb 15 13:51:15 2008 From: bill501 <@t> mindspring.com (Bill) Date: Fri Feb 15 13:51:22 2008 Subject: FW: [Histonet] Food and drink? In-Reply-To: <5F31F38C96781A4FBE3196EBC22D47807F250B@fhosxchmb006.ADVENTISTCORP.NET> References: <000401c86fed$d04bf3f0$d00f7ca5@lurie.northwestern.edu> <5F31F38C96781A4FBE3196EBC22D47807F250B@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: Sounds like a bourbon legend to me. Any evidence to back up this claim? Several and 3 dying-- hmmmm. More likely they were cleaning their 'tomes with carbon tet while swilling lab alcohol. BB At 12:56 PM -0500 2/15/08, Bonner, Janet wrote: >And this was not an arbitrary rule.....several techs at a University in the USA contracted Hepatitis from drinking their coffee which became contaminated by particulate aerosol from the specimen being worked on... and three of them died. -True story. And the beginning of of this regulation. From Janet.Bonner <@t> FLHOSP.ORG Fri Feb 15 14:13:25 2008 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Feb 15 14:14:35 2008 Subject: FW: [Histonet] Food and drink? References: <000401c86fed$d04bf3f0$d00f7ca5@lurie.northwestern.edu><5F31F38C96781A4FBE3196EBC22D47807F250B@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2510@fhosxchmb006.ADVENTISTCORP.NET> It was in the Chemistry Lab and it happened the week I was hired in the Histology Department. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bill Sent: Fri 2/15/2008 2:51 PM To: histonet@lists.utsouthwestern.edu Subject: Re: FW: [Histonet] Food and drink? Sounds like a bourbon legend to me. Any evidence to back up this claim? Several and 3 dying-- hmmmm. More likely they were cleaning their 'tomes with carbon tet while swilling lab alcohol. BB At 12:56 PM -0500 2/15/08, Bonner, Janet wrote: >And this was not an arbitrary rule.....several techs at a University in the USA contracted Hepatitis from drinking their coffee which became contaminated by particulate aerosol from the specimen being worked on... and three of them died. -True story. And the beginning of of this regulation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From akelly <@t> roseliassociates.com Fri Feb 15 16:11:21 2008 From: akelly <@t> roseliassociates.com (Dr. Andrea Kelly) Date: Fri Feb 15 16:12:08 2008 Subject: [Histonet] Invitation from Dr. Helen Moore, OBBR, NCI to attendFree BRN Sypmposium in March Message-ID: <47B60E09.8060003@roseliassociates.com> The National Cancer Institute (NCI) Office of Biorepositories and Biospecimen Research (OBBR) and the National Institutes of Health Office of Rare Diseases announce the Biospecimen Research Network (BRN) Symposium: *"/Advancing Cancer Research Through Biospecimen Science"/* *March 13-14, 2008* Renaissance M Street Hotel 1143 New Hampshire Avenue NW Washington, D.C. The primary goal of the symposium is to address the significant impact of pre-analytical biospecimen variability on cancer research and molecular medicine. The symposium will feature expert presentations and interactive discussions on topics in biospecimen science including: ? /HER2/neu/: Lessons Learned ? Access to Existing Knowledge in Biospecimen Science ? Research Advances in Biospecimen Science ? Assessing and Qualifying Biospecimen Quality ? Patient's Perspective on Biospecimen Quality ? Incorporating Biospecimen Science Into Research and Clinical Practice Key presenters to address the significant impact of biospecimen variability in cancer research and recent advances in biospecimen science include: ? Patrick O. Brown, M.D., Ph.D., Stanford University School of Medicine ? Carolyn C. Compton, M.D., Ph.D., OBBR, NCI ? Angelo deMarzo, M.D., Ph.D., Johns Hopkins University ? Theo deVos, Ph.D., Epigenomics, Inc. ? Steve Gutman, M.D., M.B.A., Food and Drug Administration ? Elizabeth Hammond, M.D., FCAP, University of Utah/LDS Hospital Daniel Hayes, M.D., University of Michigan ? Scott Jewell, Ph.D., Ohio State University ? Paula Kim, Translating Research Across Communities ? Lance Liotta, M.D, Ph.D., George Mason University ? Chris Logothetis, M.D., M.D. Anderson Cancer Center ? Scott D. Patterson, Ph.D., Amgen Inc. ? David Ransohoff, M.D., University of North Carolina at Chapel Hill ? Gerry Thomas, Ph.D. Imperial College London, United Kingdom ? Paul Waring, Ph.D., Genentech, Inc. ? and others This interactive symposium is free and open to the public and is expected to be of particular value to research investigators, clinicians, government representatives, industry representatives, and patient advocates. *For more information, to register, and to submit an abstract, please visit www.brnsymposium.com .* * * We apologize if you receive more than one copy of this notice. Please share this notice with any interested colleagues. We look forward to your participation. If you have any questions about the meeting or registration, please contact Marlene Goldman at mgoldman@md.capconcorp.com or 301-468-6004 ext. 416. Sincerely, Helen M. Moore, Ph.D. Biospecimen Research Network Program Manager Office of Biorepositories and Biospecimen Research Office of the Director National Cancer Institute 31 Center Drive, 31/10A03 Bethesda, MD 20892-2590 Phone: (301) 496-1550 Fax: (301) 480-4814 E-mail: moorehe@mail.nih.gov http://biospecimens.cancer.gov -- Posted by Andrea Kelly, Ph.D. Rose Li & Associates Contractor to NCI, OBBR From alineumann <@t> aol.com Fri Feb 15 19:02:22 2008 From: alineumann <@t> aol.com (alineumann@aol.com) Date: Fri Feb 15 19:02:33 2008 Subject: [Histonet] GMS and Bouin's fixation Message-ID: <8CA3E4EB86D7409-A58-3F46@webmail-ne10.sysops.aol.com> Hello all, has anyone ever experienced Bouin's fixed tissue?preventing the methenamine from staining fungus?? Thank you, Alice Neumann MD Western Wyoming Pathology Jackson, WY 83001 Cell: 307-413-4042 Work: 307-733-6418 Home: 307-734-4410 Fax: 307-734-0885 ________________________________________________________________________ More new features than ever. Check out the new AOL Mail ! - http://webmail.aol.com From rydomsal <@t> yahoo.com Fri Feb 15 19:52:53 2008 From: rydomsal <@t> yahoo.com (Ryan Dominique Salazar) Date: Fri Feb 15 19:53:00 2008 Subject: [Histonet] positive control tissues for Perl's and amyloid Message-ID: <748581.20440.qm@web52305.mail.re2.yahoo.com> Hi to all, Can anyone of you suggest any positive control tissues for Perl's for Ferric Iron and as well as amyloid stain? I've tried to use the liver for Perl's and skin( with muscle) for amyloid. Is there any much better than any of these tissues? My specimen are non-human primates particularly macaques. Thanks! Ryan Dominique Salazar, RMT, AMT Maccine Pte Ltd, Singapore --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From tgoodpas <@t> fhcrc.org Fri Feb 15 20:20:25 2008 From: tgoodpas <@t> fhcrc.org (Goodpaster, Tracy A) Date: Fri Feb 15 20:24:49 2008 Subject: [Histonet] Caspase-3-problem References: <5C0BED61F529364E86309CADEA63FEF20163F2F1@TRFT-EX01.xRothGen.nhs.uk> <60F7DC6E18EE4530BF33A4FFDFD1648F@Astrocito> Message-ID: Hi Alonso, We use mouse ovary/uterus for positive control for Cleaved Caspase-3. We have used either high pH retrieval buffer or Trilogy from Cell Marque for antigen retrieval (solution preheated to over 95 deg) in a steamer for 15 minutes with a cool down in the buffer for another 15 minutes. Then use a biotinylated secondary and ABC amplification. I hope this is helpful to you! Good luck! Tracy Goodpaster BA, HTL (ASCP) Experimental Histopathology, Shared Resources Fred Hutchinson Cancer Research Center ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Alonso Martinez-Canabal Sent: Fri 2/15/2008 11:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Caspase-3-problem Hi everyone, I have been trying to obtain a nice caspase-3-cleaved immunohistochemistry or immunofluorescence with frozen or vibratome sliced brains (particularly hippocampus). I have been using the cell signalling antibody, without a lot of success. I tried antigen retrieval with heating, and combined heating with proteinase K digestion, with not good results. Could you suggest me something? I also would like to know of any control tissue, I know that in normal young mouse brain should be some positive cells in the dentate gyrus, but do you kno any other structure that could be usable? Like skin, or liver, or anything like that? Thank you very much for your valuable help Alonso -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bell, Lynne Sent: February-15-08 1:37 PM To: Marshall Terry Dr, Consultant Histopathologist; Rene J Buesa; WWmn916@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Food and drink? I wear contact lenses in the lab, but I also wear safety glasses all the time. I have been wearing lenses in histology for over thirty years and have never had a problem. And yes, Terry, when I cut onions while wearing contacts, my eyes do not water. I believe they do protect your eyes!! Lynne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histology.bc <@t> shaw.ca Sat Feb 16 01:52:54 2008 From: histology.bc <@t> shaw.ca (Paul Bradbury) Date: Sat Feb 16 01:50:22 2008 Subject: [Histonet] GMS and Bouin's fixation In-Reply-To: <8CA3E4EB86D7409-A58-3F46@webmail-ne10.sysops.aol.com> References: <8CA3E4EB86D7409-A58-3F46@webmail-ne10.sysops.aol.com> Message-ID: <47B69656.6070705@shaw.ca> Hi Alice, Logically, there may be a conflict between fixing tissues in Bouin's and successful demonstration of fungi using a methemamine silver technique. This is my reasoning: Bouin has long been the recommended fixative for glycogen and proteoglygans which are best demonstrated by the PAS reaction. In the PAS, carbohydrate groups are oxidised by periodic acid to form aldehydes, which in turn react with Schiff reagent to produce the magenta colour. Periodic acid, as a 1% solution at room temperature, will oxidize these groups only as far as the aldehyde stage. The methenamine silver is essentially a modification of that same concept, but using chromic acid and an unstable silver solution in place of the periodic acid and Schiff reagent. In the methenamine silver technique, the carbohydrates in the capsule surrounding the fungal elements are oxidized to form aldehydes which reduce the silver solution to produce visible deposits of silver. Prolonged treatment with chromic acid will over-oxidize the carbohydrates to carboxyl groups which are non-reactive with the silver solution. There may well be a reaction between the picric acid in Bouin's fixative and carbohydrates. Picric acid is a potent oxidizer and may begin the oxidation of the carbohydrate groups in the fungal capsule. When the sections are further oxidized by chromic acid, the fungal walls become over-oxidized and form non-reactive carboxyl groups. It may be worth trying a much shorter chromic acid treatment on Bouin's fixed tissues to see if this will leave the carbohydrates in the fungi at the aldehyde stage. Most methenamine silver techniques suggest a 60 minutes treatment in 5% chromic acid at room temperature. In the case of Bouin-fixed tissues, I would suggest running a trial using a range of chromic acid times from 10-40 minutes to see if the fungi are still reactive with one of the shorter oxidation times. I would be very interested to know if this solves your problem. Paul Bradbury Kamloops, BC Canada alineumann@aol.com wrote: > Hello all, has anyone ever experienced Bouin's fixed tissue?preventing the methenamine from staining fungus?? Thank you, > > > Alice Neumann MD > Western Wyoming Pathology > Jackson, WY 83001 > > Cell: 307-413-4042 > Work: 307-733-6418 > Home: 307-734-4410 > Fax: 307-734-0885 > > ________________________________________________________________________ > More new features than ever. Check out the new AOL Mail ! - http://webmail.aol.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From gu.lang <@t> gmx.at Sat Feb 16 05:22:26 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Feb 16 05:22:35 2008 Subject: AW: [Histonet] positive control tissues for Perl's and amyloid In-Reply-To: <748581.20440.qm@web52305.mail.re2.yahoo.com> Message-ID: <000301c8708e$361b39c0$eeeea8c0@dielangs.at> We take spleen as positive control for prussian blue. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Ryan Dominique Salazar Gesendet: Samstag, 16. Februar 2008 02:53 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] positive control tissues for Perl's and amyloid Hi to all, Can anyone of you suggest any positive control tissues for Perl's for Ferric Iron and as well as amyloid stain? I've tried to use the liver for Perl's and skin( with muscle) for amyloid. Is there any much better than any of these tissues? My specimen are non-human primates particularly macaques. Thanks! Ryan Dominique Salazar, RMT, AMT Maccine Pte Ltd, Singapore --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Sat Feb 16 06:59:08 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sat Feb 16 06:59:12 2008 Subject: [Histonet] PAS + IF In-Reply-To: References: <582736990802141836u25754898n733d083325354a31@mail.gmail.com> Message-ID: <582736990802160459m388de996j8eacc732b7435f9f@mail.gmail.com> Vinnie, That's the first thing I told her. She knows it, but still wants to do it anyway. I think she plans to take images of the PAS after taking IF images so that the architecture is the same. There may be some image layering involved as well. Thanks, Amos On Feb 15, 2008 10:23 AM, Della Speranza, Vinnie wrote: > Amos, do you or the researcher know that basic fuchsin or its constituent > dyes (rosanilin, pararosaniline) will not fluoresce with the incident > excitation wavelength that will be used for the fluorescent tag? Aside from > the issues you raised this could make for interpretation difficulties > > Vinnie Della Speranza > > Manager for Anatomic Pathology Services > > 165 Ashley Avenue Suite 309 > > Charleston, South Carolina 29425 > > Tel: (843) 792-6353 > > Fax: (843) 792-8974 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks > Sent: Thursday, February 14, 2008 9:36 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] PAS + IF > > Hi, > There is a researcher here that is interested in doing a PAS and an > immunofluorescent label on the same slide. She is using formalin fixed > paraffin embedded tissue and the antibody requires heat induced epitope > retrieval. So obviously the PAS will need to come first as the reagents > involved would innevitably destroy the fluorescent label. The question is: > will the retrieval affect the PAS reaction? My initial guess was that the > PAS is a fairly strong bond (spill schiffs on your hand ... see what I > mean!) so it should survive, but that is just a guess. Has anyone tried > it? > > Thanks, > Amos Brooks > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jnocito <@t> satx.rr.com Sat Feb 16 08:09:40 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sat Feb 16 08:09:43 2008 Subject: [Histonet] Food and drink? References: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F537@lmhsmail.lmhealth.org> Message-ID: <005301c870a5$9258ff80$0302a8c0@yourxhtr8hvc4p> I used to chew tobacco at my microtome. Some of the girls around me didn't like me spitting in their trashcans JTT ----- Original Message ----- From: "Tom McNemar" To: Sent: Friday, February 15, 2008 9:43 AM Subject: RE: [Histonet] Food and drink? We have the same rules as those below. As far as contacts, you can wear them, just not put them in or take them out at the bench. Don't know why you would, but that's what the rule says. I (probably like many), remember the days when it was perfectly acceptable to eat, drink, and smoke at the microtome. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Marshall Terry Dr, Consultant Histopathologist Sent: Friday, February 15, 2008 8:52 AM To: Rene J Buesa; WWmn916@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Food and drink? Using contact lenses forbidden!!! You can't be serious. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 15 February 2008 13:18 To: WWmn916@aol.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Food and drink? For your information: smoking, drinking and eating, applying cosmetics, or even using contact lens are ABSOLUTELY prohibited in a medical histology laboratory setting. Should I imply by your question that you do not work in a medical lab. setting? Some universities and animal labs have been able to get away with ignoring those regulations up to now, but not any medical lab. that even have to have designated lounge areas. Ren? J. WWmn916@aol.com wrote: Dare I ask this question, Does anyone use closed topped and safety mugs at their microtome station when cutting slides...etc.? Would cutting paraffin blocks constitute Blood Borne Pathogen concerns? I fully understand not eating while paraffin is flying all over the place......but closed containers with coffee for those of us who work sleep deprived hours? Someone save us! Anonymous **************The year's hottest artists on the red carpet at the Grammy Awards. Go to AOL Music. (http://music.aol.com/grammys?NCID=aolcmp00300000002565) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ccrowder <@t> vetmed.lsu.edu Sat Feb 16 12:40:02 2008 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Sat Feb 16 12:43:55 2008 Subject: [Histonet] Iron control Message-ID: If your looking for a gorgeous iron positive control try a rabbit liver. They have free iron and stain beautifully with all iron stains. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 From yellowstar237 <@t> yahoo.com Sat Feb 16 13:46:40 2008 From: yellowstar237 <@t> yahoo.com (Jennifer Easterling) Date: Sat Feb 16 13:46:43 2008 Subject: [Histonet] Help with FISH on urine samples... Message-ID: <561398.92942.qm@web39814.mail.mud.yahoo.com> Hello everyone, I am new to this list and I have found it very helpful. Hopefully, someone can help me with my problem...There is a lab in my area wanting to start FISH on urine samples for bladder cancer. They are in the very beginning stages and have no policy or procedure manual in place. If anyone has any information on either of these two things, any help would be greatly appreciated. Thanks in advance, Jennifer --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From rjbuesa <@t> yahoo.com Sat Feb 16 15:00:04 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Feb 16 15:00:12 2008 Subject: [Histonet] Help with FISH on urine samples... In-Reply-To: <561398.92942.qm@web39814.mail.mud.yahoo.com> Message-ID: <96706.87445.qm@web61211.mail.yahoo.com> Jennifer: Try to contact a cytotechnologist in your area and s/he will tell you how to prepare cytospins from urine. With the cytospins you can prepare slides to treat with FISH for the cancer probes you want to use. Ren? J. Jennifer Easterling wrote: Hello everyone, I am new to this list and I have found it very helpful. Hopefully, someone can help me with my problem...There is a lab in my area wanting to start FISH on urine samples for bladder cancer. They are in the very beginning stages and have no policy or procedure manual in place. If anyone has any information on either of these two things, any help would be greatly appreciated. Thanks in advance, Jennifer --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From lpwenk <@t> sbcglobal.net Sat Feb 16 17:43:27 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sat Feb 16 17:43:37 2008 Subject: [Histonet] GMS and Bouin's fixation In-Reply-To: <47B69656.6070705@shaw.ca> Message-ID: <000701c870f5$baa5b6e0$0202a8c0@HPPav2> The reasoning sounds right to me. I might suggest using 0.5% periodic acid (such as in the PAS stain) for 5-10 minutes as the oxidizer for the GMS stain, instead of the chromic acid. Since the picric acid has started the carbohydrate oxidation, and the chromic acid is then overoxidizing the remaining carbohydrates, then using a mild oxidizer such as periodic acid might do the trick. Usually, periodic acid is NOT used in the GMS stain, as not enough background is overoxidized. The PASM/Jones stain used to demonstrate basement membranes in kidney biopsies is the same silver methenamine solution as in the GMS, but periodic acid is used instead of chromic acid. Our kidney biopsies are fixed in DB, which is an alcoholic-Bouins, which does contain picric acid. But the PASM/Jones stain continues to demonstrate basement membrane. If a GMS is done on the kidney biopsy, the basement membrane is not demonstrated. So I think using 0.5% periodic acid for 5-10 minutes, rinsing in d. water, and then going into the GMS methenamine silver solution should work. Peggy A. Wenk, HTL(ASC)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paul Bradbury Sent: Saturday, February 16, 2008 2:53 AM To: alineumann@aol.com; HistoNet Server Subject: [Histonet] GMS and Bouin's fixation Hi Alice, Logically, there may be a conflict between fixing tissues in Bouin's and successful demonstration of fungi using a methemamine silver technique. This is my reasoning: Bouin has long been the recommended fixative for glycogen and proteoglygans which are best demonstrated by the PAS reaction. In the PAS, carbohydrate groups are oxidised by periodic acid to form aldehydes, which in turn react with Schiff reagent to produce the magenta colour. Periodic acid, as a 1% solution at room temperature, will oxidize these groups only as far as the aldehyde stage. The methenamine silver is essentially a modification of that same concept, but using chromic acid and an unstable silver solution in place of the periodic acid and Schiff reagent. In the methenamine silver technique, the carbohydrates in the capsule surrounding the fungal elements are oxidized to form aldehydes which reduce the silver solution to produce visible deposits of silver. Prolonged treatment with chromic acid will over-oxidize the carbohydrates to carboxyl groups which are non-reactive with the silver solution. There may well be a reaction between the picric acid in Bouin's fixative and carbohydrates. Picric acid is a potent oxidizer and may begin the oxidation of the carbohydrate groups in the fungal capsule. When the sections are further oxidized by chromic acid, the fungal walls become over-oxidized and form non-reactive carboxyl groups. It may be worth trying a much shorter chromic acid treatment on Bouin's fixed tissues to see if this will leave the carbohydrates in the fungi at the aldehyde stage. Most methenamine silver techniques suggest a 60 minutes treatment in 5% chromic acid at room temperature. In the case of Bouin-fixed tissues, I would suggest running a trial using a range of chromic acid times from 10-40 minutes to see if the fungi are still reactive with one of the shorter oxidation times. I would be very interested to know if this solves your problem. Paul Bradbury Kamloops, BC Canada alineumann@aol.com wrote: > Hello all, has anyone ever experienced Bouin's fixed tissue?preventing > the methenamine from staining fungus?? Thank you, > > > Alice Neumann MD > Western Wyoming Pathology > Jackson, WY 83001 > > Cell: 307-413-4042 > Work: 307-733-6418 > Home: 307-734-4410 > Fax: 307-734-0885 > > ______________________________________________________________________ > __ More new features than ever. Check out the new AOL Mail ! - > http://webmail.aol.com _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Sun Feb 17 05:18:03 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sun Feb 17 05:19:09 2008 Subject: AW: [Histonet] Help with FISH on urine samples... In-Reply-To: <561398.92942.qm@web39814.mail.mud.yahoo.com> Message-ID: <001e01c87156$c3c06070$eeeea8c0@dielangs.at> I think, this site could help you. http://www.urovysion.com/proceduraloverview_358.aspx Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Jennifer Easterling Gesendet: Samstag, 16. Februar 2008 20:47 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Help with FISH on urine samples... Hello everyone, I am new to this list and I have found it very helpful. Hopefully, someone can help me with my problem...There is a lab in my area wanting to start FISH on urine samples for bladder cancer. They are in the very beginning stages and have no policy or procedure manual in place. If anyone has any information on either of these two things, any help would be greatly appreciated. Thanks in advance, Jennifer --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Sun Feb 17 11:24:28 2008 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Sun Feb 17 11:27:43 2008 Subject: [Histonet] Scabies Message-ID: Hi all! I have a question about the proper way to process/prepare skin scrapings for scabies. We have had a few cases in the last year, and want to be sure that they are being submitted properly. Also, does anyone know where we might purchase a positive slide/picture to identify the eggs. With something that occurs so infrequently, the pathologist's would like to have a reference so that they know what they are looking for. Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. From pdunlop720 <@t> gmail.com Sun Feb 17 13:10:59 2008 From: pdunlop720 <@t> gmail.com (Patty Dunlop) Date: Sun Feb 17 13:11:03 2008 Subject: [Histonet] HTL exam - Lab Management component Message-ID: <80ab7bc60802171110j4adb92d1i562c6739c1cb8379@mail.gmail.com> Hello, I am currently studying for the ASCP HTL exam. I want to purchase one of the recommended readings about lab management, but am unsure which one to purchase, if any. Please give feedback on either one of the following. Also, does anyone know what percentage of this information will be on the actual exam? Do I even need to purchase a book for this component? 1) Varnadoe, L.A. (1996). Medical Laboratory Management and Supervision: Operations, Review and Study Guide. 2) Wallace, M.A. & Klosinski, D.D. (1998). Clinical Laboratory Science Education & Management. Thanks, Patty From trathborne <@t> somerset-healthcare.com Sun Feb 17 13:43:00 2008 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Sun Feb 17 13:43:08 2008 Subject: [Histonet] Scabies Message-ID: > Hi all! > I have a question about the proper way to process/prepare skin scrapings for scabies. We have had a few cases in the last year, and want to be sure that they are being submitted properly. Also, does anyone know where we might purchase a positive slide/picture to identify the eggs. With something that occurs so infrequently, the pathologist's would like to have a reference so that they know what they are looking for. > > Thanks, > Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. From kdwyer3322 <@t> aol.com Sun Feb 17 13:51:05 2008 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Sun Feb 17 13:51:11 2008 Subject: [Histonet] Texas Society For Histotechnology State Meeting April 18-20, 2008 Message-ID: <8CA3FB590D76C25-119C-2664@FWM-D19.sysops.aol.com> To All, The Texas Society for Histotechnology will be hosting our annual Symposium/Convention April 18-20, 2008 at the Hilton DFW Lakes in Grapevine, Texas.? For more information and program?log onto txsh.org or contact Kathy Dwyer at kdwyer3322@aol.com or samuel.jones2@med.va.gov to receive a copy of the program.? There will be golf tournament on Thursday April 17, 2008 at 1:00pm prior to the start of the meeting.? For more information or to register to play contact: mhale@carisdx.com .? Golfing is open to all.? We hope to see you in Texas at the meeting.? ________________________________________________________________________ More new features than ever. Check out the new AOL Mail ! - http://webmail.aol.com From jmjohnson34 <@t> hotmail.com Sun Feb 17 16:05:39 2008 From: jmjohnson34 <@t> hotmail.com (Jennifer Johnson) Date: Sun Feb 17 16:05:49 2008 Subject: [Histonet] Static Cling Message-ID: Please offer any suggestions that you have for controlling static cling. My co-worker is cursed with the worst case of static I have ever seen. We have a static-free chair mat, we run two humidifiers, she lotions her hands very well, uses dryer sheets in with her scrubs, dips her blocks in a dishwasing liquid/water solution before cutting (to break the surface tension) and still the sections stick to her hands. She used a whole can of "static guard" last week, to no avail. Please HELP, she is threatening to lock the door and cut in a thong and wet T-shirt!!!!!!!!!! _________________________________________________________________ Climb to the top of the charts!?Play the word scramble challenge with star power. http://club.live.com/star_shuffle.aspx?icid=starshuffle_wlmailtextlink_jan From rjbuesa <@t> yahoo.com Sun Feb 17 16:21:37 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Feb 17 16:21:41 2008 Subject: [Histonet] Static Cling In-Reply-To: Message-ID: <678150.16558.qm@web61211.mail.yahoo.com> Could be the material (synthetic) her clothing or uniforms are made of. You could also place some dryer sheets (like Bounce) behind the disposable blades holder and in contact with it. Ren? J. Jennifer Johnson wrote: Please offer any suggestions that you have for controlling static cling. My co-worker is cursed with the worst case of static I have ever seen. We have a static-free chair mat, we run two humidifiers, she lotions her hands very well, uses dryer sheets in with her scrubs, dips her blocks in a dishwasing liquid/water solution before cutting (to break the surface tension) and still the sections stick to her hands. She used a whole can of "static guard" last week, to no avail. Please HELP, she is threatening to lock the door and cut in a thong and wet T-shirt!!!!!!!!!! _________________________________________________________________ Climb to the top of the charts! Play the word scramble challenge with star power. http://club.live.com/star_shuffle.aspx?icid=starshuffle_wlmailtextlink_jan_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From AnthonyH <@t> chw.edu.au Sun Feb 17 16:23:52 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Feb 17 16:24:34 2008 Subject: [Histonet] Food and drink? Faecal fats and the milkshake maker Message-ID: While on the topic of food and drink and using cooking utensils in the lab how about this: A Milkshake blender was used for many years to homogenise faecal fat samples. Since everyone hated the test, it was discarded a few years back. The blender was cleaned (we think) and placed in the storeroom where all old equipment goes to die. A few years later our trainees were quite vociferous and excited in the tea room. To my shock (and queasy stomach) they were making everyone milkshakes using the same blender!! My dilemma - what do I say or do (running away and seeking a career change was quite attractive at that time)? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Saturday, 16 February 2008 3:28 AM To: Rene J Buesa; WWmn916@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Food and drink? Well, spoke too soon in my last post! That strikes me as overkill. We don't have that rule in the UK (AFAIK) It's also interesting inasmuch as when I wore contacts, up until a couple of years ago when I got dry eyes, I had no problem with peeling/cutting onions. Now they irritate my eyes and make them water like hell. (Odd that dry eyes water isn't it?) This suggests that they actually protect the eyes from fumes. Anyone else had this experience? Have a good weekend all. Terry ________________________________ From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: 15 February 2008 14:50 To: Marshall Terry Dr, Consultant Histopathologist; WWmn916@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Food and drink? Tell that to those writing the regulations. The rationale being that fumes could be trapped between the cornea and the contact lens causing irritations and potentially damaging the cornea. Ren? J. "Marshall Terry Dr, Consultant Histopathologist" wrote: Using contact lenses forbidden!!! You can't be serious. Terry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: 15 February 2008 13:18 To: WWmn916@aol.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Food and drink? For your information: smoking, drinking and eating, applying cosmetics, or even using contact lens are ABSOLUTELY prohibited in a medical histology laboratory setting. Should I imply by your question that you do not work in a medical lab. setting? Some universities and animal labs have been able to get away with ignoring those regulations up to now, but not any medical lab. that even have to have designated lounge areas. Ren? J. WWmn916@aol.com wrote: Dare I ask this question, Does anyone use closed topped and safety mugs at their microtome station when cutting slides...etc.? Would cutting paraffin blocks constitute Blood Borne Pathogen concerns? I fully understand not eating while paraffin is flying all over the place......but closed containers with coffee for those of us who work sleep deprived hours? Someone save us! Anonymous **************The year's hottest artists on the red carpet at the Grammy Awards. Go to AOL Music. (http://music.aol.com/grammys?NCID=aolcmp00300000002565) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Never miss a thing. Make Yahoo your homepage. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Sun Feb 17 16:25:54 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Feb 17 16:26:32 2008 Subject: [Histonet] Food and drink? Message-ID: Do they remove their gloves, wash their hands and remove other protective gear before sipping their coffee? Might seem to defeating the purpose doesn't it? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mary A. Ascenzi Sent: Saturday, 16 February 2008 4:48 AM To: histonet@lists.utsouthwestern.edu; WWmn916@aol.com Subject: Re: [Histonet] Food and drink? Nope, can't do it. I've seen some research labs that have small shelves outside the lab door where people park their covered coffee cups so they can run out for a quick gulp. Kind of reminds me of rats pressing on levers for food pellet rewards. Mary >Dare I ask this question, >Does anyone use closed topped and safety mugs at their microtome >station when cutting slides...etc.? Would cutting paraffin blocks >constitute Blood Borne Pathogen concerns? I fully understand not >eating while paraffin is flying all over the place......but closed >containers with coffee for those of us who work sleep deprived hours? >Someone save us! > >Anonymous > > > >**************The year's hottest artists on the red carpet at the Grammy >Awards. Go to AOL Music. >(http://music.aol.com/grammys?NCID=aolcmp00300000002565) >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Sun Feb 17 16:28:32 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Feb 17 16:29:08 2008 Subject: [Histonet] Coffee Cup Message-ID: Cindy who is "Anonymous #2"? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy DuBois Sent: Saturday, 16 February 2008 5:19 AM To: WWmn916@aol.com; Histonet Subject: [Histonet] Coffee Cup We have closed top coffee cups while embedding, but not cutting. P.S. Don't tell the safety compliance officer Anonymous #2 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Sun Feb 17 16:29:07 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Feb 17 16:29:42 2008 Subject: [Histonet] Food and drink? Message-ID: Why? To protect us from the pathologists? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Markus F. Meyenhofer Sent: Saturday, 16 February 2008 5:30 AM To: Ingles Claire Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Food and drink? The next GREAT Idea for the "lab police": Hazmat suits for histotechs!!! ----- Original Message ----- From: "Ingles Claire" Cc: Sent: Friday, February 15, 2008 1:08 PM Subject: RE: [Histonet] Food and drink? Yea, I was getting nowhere with my Doc walking through with his coffee, snacks, etc.(those of you who know me, know how fiesty I can get.) I finally had to get my supervisor to talk to him. That would have been great for a CLIA/JCAHO inspection. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Fri 2/15/2008 9:03 AM To: Victoria Baker; WWmn916@aol.com Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Food and drink? Victoria: Your annecdote with the surgeon, reminds me a similar case. The surgeon in my case, as almost all of them, used "to confer with God every other Thursday". I spoke with the hospital director, the surgeon got a written reprimand, never ever broke the rule again, neither any of the members of my staff. Ren? J. Victoria Baker wrote: --------------------------------- Never miss a thing. Make Yahoo your homepage. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From max_histo_00 <@t> yahoo.it Mon Feb 18 05:23:36 2008 From: max_histo_00 <@t> yahoo.it (Massimo) Date: Mon Feb 18 05:23:46 2008 Subject: [Histonet] =?iso-8859-1?q?95=B0_alcohol_substitute?= Message-ID: <660210.75551.qm@web23311.mail.ird.yahoo.com> Dear listers, sometime it happens, with some staining procedures, for instance Van Gieson, that would be necessary to wash or to differentiate many times in 95? alcohol. I wonder if would be possible to perform the same operations with denatured alcohol (methylated spirit), definitely more cheaper than the first one. What do you think about? Thank you very much beforehand. Best Regards, Massimo --------------------------------- --------------------------------- L'email della prossima generazione? Puoi averla con la nuova Yahoo! Mail From JWEEMS <@t> sjha.org Mon Feb 18 08:13:28 2008 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Mon Feb 18 08:13:34 2008 Subject: [Histonet] Static Cling In-Reply-To: References: Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD320518E6B5@sjhaexc02.sjha.org> Do you have a faucet of something near by that you can run a ground wire to? You can use thin wire attached to the microtome and twist it around the faucet or air or gas connect. I have no idea where I heard this - may be farm logic (I grew up a farmer), but I used to do it and it worked! And I just read in a household hint column that attaching a safety pin to a seam would eliminate static cling in clothing. Good luck. There's nothing much more aggrevating than that problem! j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Johnson Sent: Sunday, February 17, 2008 5:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Static Cling Please offer any suggestions that you have for controlling static cling. My co-worker is cursed with the worst case of static I have ever seen. We have a static-free chair mat, we run two humidifiers, she lotions her hands very well, uses dryer sheets in with her scrubs, dips her blocks in a dishwasing liquid/water solution before cutting (to break the surface tension) and still the sections stick to her hands. She used a whole can of "static guard" last week, to no avail. Please HELP, she is threatening to lock the door and cut in a thong and wet T-shirt!!!!!!!!!! _________________________________________________________________ Climb to the top of the charts!?Play the word scramble challenge with star power. http://club.live.com/star_shuffle.aspx?icid=starshuffle_wlmailtextlink_jan_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From boligy <@t> musc.edu Mon Feb 18 09:47:08 2008 From: boligy <@t> musc.edu (Bolig, Yuriko Durst) Date: Mon Feb 18 09:46:37 2008 Subject: [Histonet] RE: Suggestions for RCC and Caldesmon antibodies In-Reply-To: <5teknr$8d3jtj@ironp1.musc.edu> References: <5teknr$8d3jtj@ironp1.musc.edu> Message-ID: <2D49A40FD22C514DA86E7F25BF83B555B903F859AA@EVS4.clinlan.local> Can anyone suggest which antibodies to use for RCC and Caldesmon? We have tried Dako's RCC and want to try another company. The Caldesmon is new for us, so any suggestion is greatly appreciated. Thank you for your time! Yudi Bolig -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Sunday, February 17, 2008 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 51, Issue 26 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Iron control (Cheryl Crowder) 2. Help with FISH on urine samples... (Jennifer Easterling) 3. Re: Help with FISH on urine samples... (Rene J Buesa) 4. RE: GMS and Bouin's fixation (Lee & Peggy Wenk) 5. AW: [Histonet] Help with FISH on urine samples... (Gudrun Lang) 6. Scabies (Rathborne, Toni) ---------------------------------------------------------------------- Message: 1 Date: Sat, 16 Feb 2008 12:40:02 -0600 From: "Cheryl Crowder" Subject: [Histonet] Iron control To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" If your looking for a gorgeous iron positive control try a rabbit liver. They have free iron and stain beautifully with all iron stains. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 ------------------------------ Message: 2 Date: Sat, 16 Feb 2008 11:46:40 -0800 (PST) From: Jennifer Easterling Subject: [Histonet] Help with FISH on urine samples... To: histonet@lists.utsouthwestern.edu Message-ID: <561398.92942.qm@web39814.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello everyone, I am new to this list and I have found it very helpful. Hopefully, someone can help me with my problem...There is a lab in my area wanting to start FISH on urine samples for bladder cancer. They are in the very beginning stages and have no policy or procedure manual in place. If anyone has any information on either of these two things, any help would be greatly appreciated. Thanks in advance, Jennifer --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. ------------------------------ Message: 3 Date: Sat, 16 Feb 2008 13:00:04 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Help with FISH on urine samples... To: Jennifer Easterling , histonet@lists.utsouthwestern.edu Message-ID: <96706.87445.qm@web61211.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Jennifer: Try to contact a cytotechnologist in your area and s/he will tell you how to prepare cytospins from urine. With the cytospins you can prepare slides to treat with FISH for the cancer probes you want to use. Ren? J. Jennifer Easterling wrote: Hello everyone, I am new to this list and I have found it very helpful. Hopefully, someone can help me with my problem...There is a lab in my area wanting to start FISH on urine samples for bladder cancer. They are in the very beginning stages and have no policy or procedure manual in place. If anyone has any information on either of these two things, any help would be greatly appreciated. Thanks in advance, Jennifer --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. ------------------------------ Message: 4 Date: Sat, 16 Feb 2008 18:43:27 -0500 From: "Lee & Peggy Wenk" Subject: RE: [Histonet] GMS and Bouin's fixation To: "'Paul Bradbury'" , , "'HistoNet Server'" Message-ID: <000701c870f5$baa5b6e0$0202a8c0@HPPav2> Content-Type: text/plain; charset="us-ascii" The reasoning sounds right to me. I might suggest using 0.5% periodic acid (such as in the PAS stain) for 5-10 minutes as the oxidizer for the GMS stain, instead of the chromic acid. Since the picric acid has started the carbohydrate oxidation, and the chromic acid is then overoxidizing the remaining carbohydrates, then using a mild oxidizer such as periodic acid might do the trick. Usually, periodic acid is NOT used in the GMS stain, as not enough background is overoxidized. The PASM/Jones stain used to demonstrate basement membranes in kidney biopsies is the same silver methenamine solution as in the GMS, but periodic acid is used instead of chromic acid. Our kidney biopsies are fixed in DB, which is an alcoholic-Bouins, which does contain picric acid. But the PASM/Jones stain continues to demonstrate basement membrane. If a GMS is done on the kidney biopsy, the basement membrane is not demonstrated. So I think using 0.5% periodic acid for 5-10 minutes, rinsing in d. water, and then going into the GMS methenamine silver solution should work. Peggy A. Wenk, HTL(ASC)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paul Bradbury Sent: Saturday, February 16, 2008 2:53 AM To: alineumann@aol.com; HistoNet Server Subject: [Histonet] GMS and Bouin's fixation Hi Alice, Logically, there may be a conflict between fixing tissues in Bouin's and successful demonstration of fungi using a methemamine silver technique. This is my reasoning: Bouin has long been the recommended fixative for glycogen and proteoglygans which are best demonstrated by the PAS reaction. In the PAS, carbohydrate groups are oxidised by periodic acid to form aldehydes, which in turn react with Schiff reagent to produce the magenta colour. Periodic acid, as a 1% solution at room temperature, will oxidize these groups only as far as the aldehyde stage. The methenamine silver is essentially a modification of that same concept, but using chromic acid and an unstable silver solution in place of the periodic acid and Schiff reagent. In the methenamine silver technique, the carbohydrates in the capsule surrounding the fungal elements are oxidized to form aldehydes which reduce the silver solution to produce visible deposits of silver. Prolonged treatment with chromic acid will over-oxidize the carbohydrates to carboxyl groups which are non-reactive with the silver solution. There may well be a reaction between the picric acid in Bouin's fixative and carbohydrates. Picric acid is a potent oxidizer and may begin the oxidation of the carbohydrate groups in the fungal capsule. When the sections are further oxidized by chromic acid, the fungal walls become over-oxidized and form non-reactive carboxyl groups. It may be worth trying a much shorter chromic acid treatment on Bouin's fixed tissues to see if this will leave the carbohydrates in the fungi at the aldehyde stage. Most methenamine silver techniques suggest a 60 minutes treatment in 5% chromic acid at room temperature. In the case of Bouin-fixed tissues, I would suggest running a trial using a range of chromic acid times from 10-40 minutes to see if the fungi are still reactive with one of the shorter oxidation times. I would be very interested to know if this solves your problem. Paul Bradbury Kamloops, BC Canada alineumann@aol.com wrote: > Hello all, has anyone ever experienced Bouin's fixed tissue?preventing > the methenamine from staining fungus?? Thank you, > > > Alice Neumann MD > Western Wyoming Pathology > Jackson, WY 83001 > > Cell: 307-413-4042 > Work: 307-733-6418 > Home: 307-734-4410 > Fax: 307-734-0885 > > ______________________________________________________________________ > __ More new features than ever. Check out the new AOL Mail ! - > http://webmail.aol.com _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Sun, 17 Feb 2008 12:18:03 +0100 From: "Gudrun Lang" Subject: AW: [Histonet] Help with FISH on urine samples... To: "'Jennifer Easterling'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <001e01c87156$c3c06070$eeeea8c0@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" I think, this site could help you. http://www.urovysion.com/proceduraloverview_358.aspx Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Jennifer Easterling Gesendet: Samstag, 16. Februar 2008 20:47 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Help with FISH on urine samples... Hello everyone, I am new to this list and I have found it very helpful. Hopefully, someone can help me with my problem...There is a lab in my area wanting to start FISH on urine samples for bladder cancer. They are in the very beginning stages and have no policy or procedure manual in place. If anyone has any information on either of these two things, any help would be greatly appreciated. Thanks in advance, Jennifer --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Sun, 17 Feb 2008 12:24:28 -0500 From: "Rathborne, Toni" Subject: [Histonet] Scabies To: Message-ID: Content-Type: text/plain; charset="utf-8" Hi all! I have a question about the proper way to process/prepare skin scrapings for scabies. We have had a few cases in the last year, and want to be sure that they are being submitted properly. Also, does anyone know where we might purchase a positive slide/picture to identify the eggs. With something that occurs so infrequently, the pathologist's would like to have a reference so that they know what they are looking for. Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 51, Issue 26 **************************************** From liz <@t> premierlab.com Mon Feb 18 10:39:00 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Mon Feb 18 10:39:05 2008 Subject: [Histonet] RE: Suggestions for RCC and Caldesmon antibodies In-Reply-To: <0961DF356D8F4CB28EF9E65804021120@PremierLab.local> References: <5teknr$8d3jtj@ironp1.musc.edu> <0961DF356D8F4CB28EF9E65804021120@PremierLab.local> Message-ID: Yudi We get our caldesmon from: Caldesmon Santa Cruz sc-52991 SPM108 We have only used it in canine tissue, but it works well. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bolig, Yuriko Durst Sent: Monday, February 18, 2008 9:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Suggestions for RCC and Caldesmon antibodies Can anyone suggest which antibodies to use for RCC and Caldesmon? We have tried Dako's RCC and want to try another company. The Caldesmon is new for us, so any suggestion is greatly appreciated. Thank you for your time! Yudi Bolig -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Sunday, February 17, 2008 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 51, Issue 26 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Iron control (Cheryl Crowder) 2. Help with FISH on urine samples... (Jennifer Easterling) 3. Re: Help with FISH on urine samples... (Rene J Buesa) 4. RE: GMS and Bouin's fixation (Lee & Peggy Wenk) 5. AW: [Histonet] Help with FISH on urine samples... (Gudrun Lang) 6. Scabies (Rathborne, Toni) ---------------------------------------------------------------------- Message: 1 Date: Sat, 16 Feb 2008 12:40:02 -0600 From: "Cheryl Crowder" Subject: [Histonet] Iron control To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" If your looking for a gorgeous iron positive control try a rabbit liver. They have free iron and stain beautifully with all iron stains. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 ------------------------------ Message: 2 Date: Sat, 16 Feb 2008 11:46:40 -0800 (PST) From: Jennifer Easterling Subject: [Histonet] Help with FISH on urine samples... To: histonet@lists.utsouthwestern.edu Message-ID: <561398.92942.qm@web39814.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello everyone, I am new to this list and I have found it very helpful. Hopefully, someone can help me with my problem...There is a lab in my area wanting to start FISH on urine samples for bladder cancer. They are in the very beginning stages and have no policy or procedure manual in place. If anyone has any information on either of these two things, any help would be greatly appreciated. Thanks in advance, Jennifer --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. ------------------------------ Message: 3 Date: Sat, 16 Feb 2008 13:00:04 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Help with FISH on urine samples... To: Jennifer Easterling , histonet@lists.utsouthwestern.edu Message-ID: <96706.87445.qm@web61211.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Jennifer: Try to contact a cytotechnologist in your area and s/he will tell you how to prepare cytospins from urine. With the cytospins you can prepare slides to treat with FISH for the cancer probes you want to use. Ren? J. Jennifer Easterling wrote: Hello everyone, I am new to this list and I have found it very helpful. Hopefully, someone can help me with my problem...There is a lab in my area wanting to start FISH on urine samples for bladder cancer. They are in the very beginning stages and have no policy or procedure manual in place. If anyone has any information on either of these two things, any help would be greatly appreciated. Thanks in advance, Jennifer --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. ------------------------------ Message: 4 Date: Sat, 16 Feb 2008 18:43:27 -0500 From: "Lee & Peggy Wenk" Subject: RE: [Histonet] GMS and Bouin's fixation To: "'Paul Bradbury'" , , "'HistoNet Server'" Message-ID: <000701c870f5$baa5b6e0$0202a8c0@HPPav2> Content-Type: text/plain; charset="us-ascii" The reasoning sounds right to me. I might suggest using 0.5% periodic acid (such as in the PAS stain) for 5-10 minutes as the oxidizer for the GMS stain, instead of the chromic acid. Since the picric acid has started the carbohydrate oxidation, and the chromic acid is then overoxidizing the remaining carbohydrates, then using a mild oxidizer such as periodic acid might do the trick. Usually, periodic acid is NOT used in the GMS stain, as not enough background is overoxidized. The PASM/Jones stain used to demonstrate basement membranes in kidney biopsies is the same silver methenamine solution as in the GMS, but periodic acid is used instead of chromic acid. Our kidney biopsies are fixed in DB, which is an alcoholic-Bouins, which does contain picric acid. But the PASM/Jones stain continues to demonstrate basement membrane. If a GMS is done on the kidney biopsy, the basement membrane is not demonstrated. So I think using 0.5% periodic acid for 5-10 minutes, rinsing in d. water, and then going into the GMS methenamine silver solution should work. Peggy A. Wenk, HTL(ASC)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paul Bradbury Sent: Saturday, February 16, 2008 2:53 AM To: alineumann@aol.com; HistoNet Server Subject: [Histonet] GMS and Bouin's fixation Hi Alice, Logically, there may be a conflict between fixing tissues in Bouin's and successful demonstration of fungi using a methemamine silver technique. This is my reasoning: Bouin has long been the recommended fixative for glycogen and proteoglygans which are best demonstrated by the PAS reaction. In the PAS, carbohydrate groups are oxidised by periodic acid to form aldehydes, which in turn react with Schiff reagent to produce the magenta colour. Periodic acid, as a 1% solution at room temperature, will oxidize these groups only as far as the aldehyde stage. The methenamine silver is essentially a modification of that same concept, but using chromic acid and an unstable silver solution in place of the periodic acid and Schiff reagent. In the methenamine silver technique, the carbohydrates in the capsule surrounding the fungal elements are oxidized to form aldehydes which reduce the silver solution to produce visible deposits of silver. Prolonged treatment with chromic acid will over-oxidize the carbohydrates to carboxyl groups which are non-reactive with the silver solution. There may well be a reaction between the picric acid in Bouin's fixative and carbohydrates. Picric acid is a potent oxidizer and may begin the oxidation of the carbohydrate groups in the fungal capsule. When the sections are further oxidized by chromic acid, the fungal walls become over-oxidized and form non-reactive carboxyl groups. It may be worth trying a much shorter chromic acid treatment on Bouin's fixed tissues to see if this will leave the carbohydrates in the fungi at the aldehyde stage. Most methenamine silver techniques suggest a 60 minutes treatment in 5% chromic acid at room temperature. In the case of Bouin-fixed tissues, I would suggest running a trial using a range of chromic acid times from 10-40 minutes to see if the fungi are still reactive with one of the shorter oxidation times. I would be very interested to know if this solves your problem. Paul Bradbury Kamloops, BC Canada alineumann@aol.com wrote: > Hello all, has anyone ever experienced Bouin's fixed tissue?preventing > the methenamine from staining fungus?? Thank you, > > > Alice Neumann MD > Western Wyoming Pathology > Jackson, WY 83001 > > Cell: 307-413-4042 > Work: 307-733-6418 > Home: 307-734-4410 > Fax: 307-734-0885 > > ______________________________________________________________________ > __ More new features than ever. Check out the new AOL Mail ! - > http://webmail.aol.com _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Sun, 17 Feb 2008 12:18:03 +0100 From: "Gudrun Lang" Subject: AW: [Histonet] Help with FISH on urine samples... To: "'Jennifer Easterling'" Cc: histonet@lists.utsouthwestern.edu Message-ID: <001e01c87156$c3c06070$eeeea8c0@dielangs.at> Content-Type: text/plain; charset="iso-8859-1" I think, this site could help you. http://www.urovysion.com/proceduraloverview_358.aspx Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Jennifer Easterling Gesendet: Samstag, 16. Februar 2008 20:47 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Help with FISH on urine samples... Hello everyone, I am new to this list and I have found it very helpful. Hopefully, someone can help me with my problem...There is a lab in my area wanting to start FISH on urine samples for bladder cancer. They are in the very beginning stages and have no policy or procedure manual in place. If anyone has any information on either of these two things, any help would be greatly appreciated. Thanks in advance, Jennifer --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Sun, 17 Feb 2008 12:24:28 -0500 From: "Rathborne, Toni" Subject: [Histonet] Scabies To: Message-ID: Content-Type: text/plain; charset="utf-8" Hi all! I have a question about the proper way to process/prepare skin scrapings for scabies. We have had a few cases in the last year, and want to be sure that they are being submitted properly. Also, does anyone know where we might purchase a positive slide/picture to identify the eggs. With something that occurs so infrequently, the pathologist's would like to have a reference so that they know what they are looking for. Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 51, Issue 26 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sqd3f <@t> cms.mail.virginia.edu Mon Feb 18 11:01:09 2008 From: sqd3f <@t> cms.mail.virginia.edu (Sonny Duong) Date: Mon Feb 18 11:01:14 2008 Subject: [Histonet] Sudan III Message-ID: Hi all! I am attempting to perform fecal fat microscopy with Sudan III stain. My protocol requires me to have 1% Sudan III dye, and my question is, 1% in what? The dye is insoluble in water, so what should I dissolve the stain in? Ehtanol? Chloroform?? Thanks, Sonny Duong Carla Green Lab University of Virginia From rjbuesa <@t> yahoo.com Mon Feb 18 11:22:08 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Feb 18 11:22:11 2008 Subject: [Histonet] Sudan III In-Reply-To: Message-ID: <407728.91069.qm@web61214.mail.yahoo.com> Sonny: Sudan III is prepared in 70% ethanol, but its solubility is a bout 0.2 g/100 mL so you will end with an oversaturated solution = with undissolved dye in the bottom of the flask. Ren? J. Sonny Duong wrote: Hi all! I am attempting to perform fecal fat microscopy with Sudan III stain. My protocol requires me to have 1% Sudan III dye, and my question is, 1% in what? The dye is insoluble in water, so what should I dissolve the stain in? Ehtanol? Chloroform?? Thanks, Sonny Duong Carla Green Lab University of Virginia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From doug <@t> ppspath.com Mon Feb 18 11:22:40 2008 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Mon Feb 18 11:23:29 2008 Subject: [Histonet] Pregnancy Message-ID: No not me.. but. What are the regulations/guidelines for working in a histology lab while one is pregnant? Is this an individual choice or a doctor choice? Are there any liabilities if something happens? Thanks. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. From liz <@t> premierlab.com Mon Feb 18 11:45:25 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Mon Feb 18 11:45:28 2008 Subject: [Histonet] Pregnancy In-Reply-To: <7C263DCC094F4AF0A0ECA6CC40460C9D@PremierLab.local> References: <7C263DCC094F4AF0A0ECA6CC40460C9D@PremierLab.local> Message-ID: Douglas If I had a tech that was pregnant I would keep them out of the lab. One of our local pharmaceutical companies had a tech that was pregnant and they were told they could not work in the lab. I'm not sure if there are any regs associated with this, but for me personally and as a business owner I would just prefer to keep them out of the lab. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Monday, February 18, 2008 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pregnancy No not me.. but. What are the regulations/guidelines for working in a histology lab while one is pregnant? Is this an individual choice or a doctor choice? Are there any liabilities if something happens? Thanks. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Mon Feb 18 12:00:44 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Mon Feb 18 12:00:58 2008 Subject: [Histonet] Static Cling In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD320518E6B5@sjhaexc02.sjha.org> References: <1CD6831EB9B26D45B0A3EAA79F7EBD320518E6B5@sjhaexc02.sjha.org> Message-ID: <5b6eb13e0802181000q64b09e7ele4fff502971ac9b0@mail.gmail.com> Have you thought of getting a humidifier? Mark Tarango On Feb 18, 2008 6:13 AM, Weems, Joyce wrote: > Do you have a faucet of something near by that you can run a ground wire > to? You can use thin wire attached to the microtome and twist it around the > faucet or air or gas connect. I have no idea where I heard this - may be > farm logic (I grew up a farmer), but I used to do it and it worked! And I > just read in a household hint column that attaching a safety pin to a seam > would eliminate static cling in clothing. > > Good luck. There's nothing much more aggrevating than that problem! j > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Johnson > Sent: Sunday, February 17, 2008 5:06 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Static Cling > > > Please offer any suggestions that you have for controlling static cling. > My co-worker is cursed with the worst case of static I have ever seen. We > have a static-free chair mat, we run two humidifiers, she lotions her hands > very well, uses dryer sheets in with her scrubs, dips her blocks in a > dishwasing liquid/water solution before cutting (to break the surface > tension) and still the sections stick to her hands. She used a whole can of > "static guard" last week, to no avail. Please HELP, she is threatening to > lock the door and cut in a thong and wet T-shirt!!!!!!!!!! > _________________________________________________________________ > Climb to the top of the charts! Play the word scramble challenge with star > power. > > http://club.live.com/star_shuffle.aspx?icid=starshuffle_wlmailtextlink_jan_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice ** The information contained in this message may be > privileged and is confidential information intended for the use of the > addressee listed above. If you are neither the intended recipient nor the > employee or agent responsible for delivering this message to the intended > recipient, you are hereby notified that any disclosure, copying, > distribution or the taking of any action in reliance on the contents of this > information is strictly prohibited. If you have received this communication > in error, please notify us immediately by replying to the message and > deleting it from your computer. Thank you. Saint Joseph's Health System, > Inc. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From tanisha.mcknight <@t> covance.com Mon Feb 18 12:15:14 2008 From: tanisha.mcknight <@t> covance.com (McKnight, Tanisha) Date: Mon Feb 18 12:15:30 2008 Subject: [Histonet] Cost comparison of Microwave Tissue Processing and Standard VIP P rocessing Message-ID: <816E3C72F855F14985FC31D7C963AE6F049EA863@indexch03.ent.covance.com> Hello all: I am putting together a proposal, and I was wondering if anyone has done a cost comparison of Microwave Tissue Processing VS. Standard VIP? Also, If anyone has any experience or recommendations about specific microwaves or protocols, that would be great too. Thanks, Tanisha, HT (ASCP) ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From GDawson <@t> dynacaremilwaukee.com Mon Feb 18 13:04:10 2008 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Mon Feb 18 13:04:20 2008 Subject: [Histonet] Pregnancy In-Reply-To: Message-ID: All, Wouldn't keeping a pregnant worker out of the lab be a form of discrimination? I've worked many different jobs (employed by Manpower temp services in college) and I can honestly say that my current lab would easily be the "healthiest" environment that I've worked in so far. Perhaps if ventilation is poor and chemical spills are commonplace in a lab, it's not the place for a pregnant woman. But then again, if the lab is too dangerous for a pregnant person, maybe it's too dangerous for all of us. Sort of reminds me of those "Baby On Board" signs for your car that encourage drivers to run into another car since they don't have a baby riding along. Just a Thought, Glen Dawson IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Liz Chlipala Sent: Monday, February 18, 2008 11:45 AM To: Douglas D Deltour; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Pregnancy Douglas If I had a tech that was pregnant I would keep them out of the lab. One of our local pharmaceutical companies had a tech that was pregnant and they were told they could not work in the lab. I'm not sure if there are any regs associated with this, but for me personally and as a business owner I would just prefer to keep them out of the lab. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Monday, February 18, 2008 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pregnancy No not me.. but. What are the regulations/guidelines for working in a histology lab while one is pregnant? Is this an individual choice or a doctor choice? Are there any liabilities if something happens? Thanks. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lee.hemo_de_2000 <@t> yahoo.com Mon Feb 18 13:42:59 2008 From: lee.hemo_de_2000 <@t> yahoo.com (Lee Sloan) Date: Mon Feb 18 14:03:42 2008 Subject: [Histonet] Pregnancy Message-ID: <746327.48581.qm@web45916.mail.sp1.yahoo.com> Howdy Folks from the great state of Texas: In a previous life, I was Human Resources/Environmental Safety manager. Thank goodness, I got smart and got out of that business. If this situation were to occur in my facility, I would make sure that all of the ducks are in a row. First I would get a note listing any workplace restrictions for the expectant mother Second...I would make sure that the treating doctor was able to take a look at the job hazard analysis of that particular workstation. This JHA should list any known airborne contaminants or any other detailed hazardous conditions along with appropriate documented readings...I would also consult any MSDS's for the chemicals that are used in that workarea...I would bundle all of that up and deliver it to the doctor and make sure that the treating doctor is able to review it and sign off on it... However to be on the humane side of things...if your facility can afford it, I would move the expectant mother outta that laboratory situation into another similiar position with the same pay and possibly even better working hours and better working conditions for the duration of the pregnancy. ***If you moved the new mother into say a front office position or another non laboratory position, that is STILL in the same physical building...to cover all the bases, I would still invest in a neutral 3rd party air borne samplings to ensure that she isn't in contact with any bad stuff....this shows good intent on your part*** Douglas D Deltour wrote: No not me.. but. What are the regulations/guidelines for working in a histology lab while one is pregnant? Is this an individual choice or a doctor choice? Are there any liabilities if something happens? Thanks. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Thanks for your business, Lee W. Sloan, Sales Manager Scientific Safety Solvents Keller, Texas 76248 USA ph 1-817-379-7328 email: lee.hemo_de_2000@yahoo.com fax: 1-817-431-5611 Hemo-De; An organic product that is a safer alternative to Xylene --------------------------------- Never miss a thing. Make Yahoo your homepage. From rjbuesa <@t> yahoo.com Mon Feb 18 14:30:07 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Feb 18 14:30:12 2008 Subject: [Histonet] Pregnancy Message-ID: <586957.38203.qm@web61216.mail.yahoo.com> Douglas: All chemicals used in the lab have TWA values LOWER for pregnant women and infants, and that is the reason why pregnant women should not work in the lab environment, regardles it may have TWA levels for adults within the limits. Ren? J. Douglas D Deltour wrote: No not me.. but. What are the regulations/guidelines for working in a histology lab while one is pregnant? Is this an individual choice or a doctor choice? Are there any liabilities if something happens? Thanks. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From andrea.stritmatter <@t> mdsinc.com Mon Feb 18 14:46:18 2008 From: andrea.stritmatter <@t> mdsinc.com (Stritmatter, Andrea) Date: Mon Feb 18 14:46:28 2008 Subject: [Histonet] Pregnancy In-Reply-To: <586957.38203.qm@web61216.mail.yahoo.com> References: <586957.38203.qm@web61216.mail.yahoo.com> Message-ID: <42D66A529F6965498107BC4A6EF63FC7445383@CATOM-MDMAPUWDE.mds.mdsinc.com> As a new mother of a 7 month old son and a manager of a histology lab, you have to work with the pregnant individual on this. It is discrimination (at least in Washington state) if forbid them to work in the lab. I chose not to take x-rays or handle DAB during my pregnancy. Otherwise I worked normally in the lab. I ended up with pregnancy complications and couldn't stand all day so I did a lot of paperwork catch up too. Ultimately, it's up to the manager and the employee to work together to find duties you all agree on. Your HR department should be able to assist in this too. Andrea Stritmatter, HTL(ASCP) MDSPS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, February 18, 2008 12:30 PM To: Douglas D Deltour; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Pregnancy Importance: Low Douglas: All chemicals used in the lab have TWA values LOWER for pregnant women and infants, and that is the reason why pregnant women should not work in the lab environment, regardles it may have TWA levels for adults within the limits. Ren? J. Douglas D Deltour wrote: No not me.. but. What are the regulations/guidelines for working in a histology lab while one is pregnant? Is this an individual choice or a doctor choice? Are there any liabilities if something happens? Thanks. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Mon Feb 18 15:19:50 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Feb 18 15:19:57 2008 Subject: [Histonet] Pregnancy References: <746327.48581.qm@web45916.mail.sp1.yahoo.com> Message-ID: <006c01c87273$ff658d30$0302a8c0@yourxhtr8hvc4p> we just had a tech give birth a couple of months ago to a 91/2 lb, 19" healthy boy. She was moved out of the lab into an admin area. We set up a microtome and waterbath for her. She used to come in the lab to pick up her blocks and deliver her cut slides and that was it. After cutting, she was given paper work to do. Towards the end, she could only work 1/2 days. She was so frustrated because her belly was so big and her arms so short that she had a hard time using the microtome. But, all in all, everything worked out fine. JTT ----- Original Message ----- From: "Lee Sloan" To: Sent: Monday, February 18, 2008 1:42 PM Subject: Re: [Histonet] Pregnancy > Howdy Folks from the great state of Texas: > > In a previous life, I was Human Resources/Environmental Safety manager. > Thank goodness, I got smart and got out of that business. > > If this situation were to occur in my facility, I would make sure that > all of the ducks are in a row. First I would get a note listing any > workplace restrictions for the expectant mother Second...I would make > sure that the treating doctor was able to take a look at the job hazard > analysis of that particular workstation. This JHA should list any known > airborne contaminants or any other detailed hazardous conditions along > with appropriate documented readings...I would also consult any MSDS's for > the chemicals that are used in that workarea...I would bundle all of that > up and deliver it to the doctor and make sure that the treating doctor is > able to review it and sign off on it... > > However to be on the humane side of things...if your facility can afford > it, I would move the expectant mother outta that laboratory situation into > another similiar position with the same pay and possibly even better > working hours and better working conditions for the duration of the > pregnancy. > > ***If you moved the new mother into say a front office position or > another non laboratory position, that is STILL in the same physical > building...to cover all the bases, I would still invest in a neutral 3rd > party air borne samplings to ensure that she isn't in contact with any bad > stuff....this shows good intent on your part*** > > Douglas D Deltour wrote: > No not me.. but. > > > > What are the regulations/guidelines for working in a histology lab while > one > is pregnant? Is this an individual choice or a doctor choice? Are there > any > liabilities if something happens? Thanks. > > > > > > Douglas D. Deltour HT(ASCP) > > Histology Manager > > Professional Pathology Services, PC > > One Science Court > > Suite 200 > > Columbia, SC 29203 > > Office (803)252-1913 > > Fax (803)254-3262 > > Doug@ppspath.com > > ***************************************************** > > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of the individual or entity to > which it is addressed and may contain information that is privileged, > confidential and exempt from disclosure under applicable law. If the > reader > of this message is not the intended recipient, you are hereby notified > that > any dissemination, distribution, or copying of this communication is > strictly prohibited by law. If you have received this communication in > error, please notify me immediately. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > Thanks for your business, > > Lee W. Sloan, Sales Manager > Scientific Safety Solvents > Keller, Texas 76248 USA > > ph 1-817-379-7328 > email: lee.hemo_de_2000@yahoo.com > fax: 1-817-431-5611 > > Hemo-De; An organic product that is a safer alternative to Xylene > > > --------------------------------- > Never miss a thing. Make Yahoo your homepage. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DivaPrincess4444 <@t> aol.com Mon Feb 18 16:28:02 2008 From: DivaPrincess4444 <@t> aol.com (DivaPrincess4444@aol.com) Date: Mon Feb 18 16:28:08 2008 Subject: [Histonet] Histotechnologist Message-ID: I'm currently taking a Vertebrate Histology class at a university this semester, and I was wondering what kind of job opportunities there are for histotechnologists, what duties they would perform on a job, where they might work, and what an average salary might be. Celeste **************Ideas to please picky eaters. Watch video on AOL Living. (http://living.aol.com/video/how-to-please-your-picky-eater/rachel-campos-duffy/ 2050827?NCID=aolcmp00300000002598) From CrochiereSteve <@t> aol.com Mon Feb 18 17:43:44 2008 From: CrochiereSteve <@t> aol.com (CrochiereSteve@aol.com) Date: Mon Feb 18 17:43:58 2008 Subject: [Histonet] Now what? Message-ID: I recently began keeping track of workload units in my lab in an attempt to boost the productivity of the staff. In the past I or another tech would do approximately one half of the workload, while the other three techs would pick up the remainder between them. Since I started keeping track of who did what, the underperformers workload has increased and mine has dropped to half of what it had previously been. Sounds great, right? Well, the plan has backfired a little when the three low performers ganged up on my best tech and accused her of monopolizing the work so that they couldn't keep up. I would have expected the opposite, where they would push the lowest ranked tech into pulling their weight. This is not used in any evaluations or merit based bonus. It was just a social experiment on my part. Now my question is: What do I do to smooth out the ruffled feathers of all involved, but keep the work flowing? If I need to dole out equal portions to everyone, production will be delayed and quality will go down. If I allow those who can do more to do so, morale will suffer. Any suggestions? Steven M. Crochiere, HT(ASCP) Histology Supervisor LifePath Partners @ Mercy Medical Center Springfield, MA 01104 **************Ideas to please picky eaters. Watch video on AOL Living. (http://living.aol.com/video/how-to-please-your-picky-eater/rachel-campos-duffy/ 2050827?NCID=aolcmp00300000002598) From FMonson <@t> wcupa.edu Mon Feb 18 23:09:12 2008 From: FMonson <@t> wcupa.edu (Monson, Frederick ) Date: Mon Feb 18 23:09:09 2008 Subject: [Histonet] Histotechnologist References: Message-ID: <641CEFFC7E5B6C42AB59539653FD082301570A96@wcu-ex-emp2.PASSHE.LCL> Celeste, Please excuse me, but you should be taking the histology course to improve your observational capabilities as well as your technique in a new area of biology. If you are using a microscope to study tissues, then you are given a great oportunity to increase your confidence in your powers of observation. The rules of identification are designed for ease of learning, not inflexible memorization. For example, what anatomic/histologic feature of the esophagus would permit you to identify the region of the esophagus from which a section had been taken (upper or lower)? How would you describe a longitudinal section of bronchus in which you saw cardiac muscle fibers? Give, in one word, the histologic/anatomic explanation for the literal collapse of a lung via a pneumothorax - from any cause, including a perforated chest wall. Why does the lung collapse? Hint: the use of the word 'collapse' is misleading. [ http://www.medicinenet.com/pneumothorax/article.htm ) How would you characterize an epithelium which included both stratified squamous and islands of stratified cuboidal - even when such a thing was not described in the book? If you were shown a diagram of the junctions between cardiac muscle cells and asked to predict a function for the horizontal and the vertical(transverse) components, what would you say? Why is tooth enamel not renewable? Do collagen fibers in bone run precisely parallel to the long axis of the shaft? How are the protein components in Haversian systems comparable to the cellulose fibers in the rings of a branch or root of a tree? [This is NOT an easy one, it is MOST interesting. The possible explanations for the arrangements are even more interesting - if you can think of them.] What is birefringence? In ground bone preparations, why are the spaces once inhabited by osteocytes black in bright field viewing? [NOTE: There is an easy answer to this question, but the explanation for the phenomenon is a little more difficult.] What are the similarities of the confocal scanning laser microscope and the scanning electron microscope? [This is a trap! The answer is more than the obvious.] In what vertebrate can one see bile canaliculi with the standard student light microscope? [Unfair! Is this in the book? Will it be on the test?] How can a giraffe breathe while he is swallowing? [Where does he get these questions??] Which of the following are intimately involved in human reproduction? a. replication b. translation c. transcription d. osculation e. none of the above f. all of the above I love this stuff! Fred Monson Frederick C. Monson, PhD Technical Director, MIRTC West Chester University West Chester, PA, 19383 610-738-0437 http://lexspiac.wcupa.edu ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of DivaPrincess4444@aol.com Sent: Mon 2/18/2008 5:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histotechnologist I'm currently taking a Vertebrate Histology class at a university this semester, and I was wondering what kind of job opportunities there are for histotechnologists, what duties they would perform on a job, where they might work, and what an average salary might be. Celeste **************Ideas to please picky eaters. Watch video on AOL Living. (http://living.aol.com/video/how-to-please-your-picky-eater/rachel-campos-duffy/ 2050827?NCID=aolcmp00300000002598) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shin.fukagawa <@t> gmail.com Tue Feb 19 06:18:13 2008 From: shin.fukagawa <@t> gmail.com (Shin Fukagawa) Date: Tue Feb 19 06:18:17 2008 Subject: [Histonet] Buccal Mucosa Smear Method using Cresyl ECHT Violet Stain Message-ID: <1cb173ee0802190418n181e508alf04d39584537596f@mail.gmail.com> Good day. Can you pls help me out. This is for my Histopathology subject.... May I ask for the procedure on how the buccal mucosa smear is collected and the method on how it is made(If there are other techniques can you just tell me the reference method). May I also ask about Cresyl ECHT stain's advantages and disadvantages and the procedure(Best if possible or reference) and the colors that it will produce and lastly can you pls. state your references... Thank You Very Much -Shin- From rjbuesa <@t> yahoo.com Tue Feb 19 07:54:34 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 19 07:54:39 2008 Subject: [Histonet] Now what? In-Reply-To: Message-ID: <128142.74393.qm@web61211.mail.yahoo.com> Divide the workload equally amongst all and keep track of finishing time and quality. The"excess time" (time left after everything is finished by all) use it for training in special procedures and tasks arounf the lab. You will have shortened the TAT for the pathologists and hopefully everybody will be happy. And you will have also increased productivity since the tasks will be completed in less time. Ren? J. CrochiereSteve@aol.com wrote: I recently began keeping track of workload units in my lab in an attempt to boost the productivity of the staff. In the past I or another tech would do approximately one half of the workload, while the other three techs would pick up the remainder between them. Since I started keeping track of who did what, the underperformers workload has increased and mine has dropped to half of what it had previously been. Sounds great, right? Well, the plan has backfired a little when the three low performers ganged up on my best tech and accused her of monopolizing the work so that they couldn't keep up. I would have expected the opposite, where they would push the lowest ranked tech into pulling their weight. This is not used in any evaluations or merit based bonus. It was just a social experiment on my part. Now my question is: What do I do to smooth out the ruffled feathers of all involved, but keep the work flowing? If I need to dole out equal portions to everyone, production will be delayed and quality will go down. If I allow those who can do more to do so, morale will suffer. Any suggestions? Steven M. Crochiere, HT(ASCP) Histology Supervisor LifePath Partners @ Mercy Medical Center Springfield, MA 01104 **************Ideas to please picky eaters. Watch video on AOL Living. (http://living.aol.com/video/how-to-please-your-picky-eater/rachel-campos-duffy/ 2050827?NCID=aolcmp00300000002598) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From Janet.Bonner <@t> FLHOSP.ORG Tue Feb 19 08:33:37 2008 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Tue Feb 19 08:34:16 2008 Subject: [Histonet] Static Cling References: Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F251A@fhosxchmb006.ADVENTISTCORP.NET> Perhaps if she started taking some antioxidants?? ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jennifer Johnson Sent: Sun 2/17/2008 5:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Static Cling Please offer any suggestions that you have for controlling static cling. My co-worker is cursed with the worst case of static I have ever seen. We have a static-free chair mat, we run two humidifiers, she lotions her hands very well, uses dryer sheets in with her scrubs, dips her blocks in a dishwasing liquid/water solution before cutting (to break the surface tension) and still the sections stick to her hands. She used a whole can of "static guard" last week, to no avail. Please HELP, she is threatening to lock the door and cut in a thong and wet T-shirt!!!!!!!!!! _________________________________________________________________ Climb to the top of the charts! Play the word scramble challenge with star power. http://club.live.com/star_shuffle.aspx?icid=starshuffle_wlmailtextlink_jan_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From Janet.Bonner <@t> FLHOSP.ORG Tue Feb 19 08:47:35 2008 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Tue Feb 19 08:49:02 2008 Subject: [Histonet] Static Cling References: Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F251D@fhosxchmb006.ADVENTISTCORP.NET> Actually, I wonder if the mat is the culprit? Could it be insulating her from the ion-release through the floor? ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jennifer Johnson Sent: Sun 2/17/2008 5:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Static Cling Please offer any suggestions that you have for controlling static cling. My co-worker is cursed with the worst case of static I have ever seen. We have a static-free chair mat, we run two humidifiers, she lotions her hands very well, uses dryer sheets in with her scrubs, dips her blocks in a dishwasing liquid/water solution before cutting (to break the surface tension) and still the sections stick to her hands. She used a whole can of "static guard" last week, to no avail. Please HELP, she is threatening to lock the door and cut in a thong and wet T-shirt!!!!!!!!!! _________________________________________________________________ Climb to the top of the charts! Play the word scramble challenge with star power. http://club.live.com/star_shuffle.aspx?icid=starshuffle_wlmailtextlink_jan_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From vazquezr <@t> ohsu.edu Tue Feb 19 09:12:30 2008 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Tue Feb 19 09:12:55 2008 Subject: [Histonet] Pregnancy Message-ID: I wish this precautions were taken with me when I worked in Portland, OR I had a problem pregnancy and still required to change the Tissue Tek chemicals, because "it wasn't fair". I lost my baby at 5 ? months. And was devastated, but still had to be back to work the next day, as not to put anyone out as to pick up my load. Plus there is more, but won't go into it. Needless to say, my manager NEVER stepped in to help me. Some managers need to be more proactive with their pregnant employees, regardless if is discriminating, use common sense. >>> "Stritmatter, Andrea" 2/18/2008 12:46 PM >>> As a new mother of a 7 month old son and a manager of a histology lab, you have to work with the pregnant individual on this. It is discrimination (at least in Washington state) if forbid them to work in the lab. I chose not to take x-rays or handle DAB during my pregnancy. Otherwise I worked normally in the lab. I ended up with pregnancy complications and couldn't stand all day so I did a lot of paperwork catch up too. Ultimately, it's up to the manager and the employee to work together to find duties you all agree on. Your HR department should be able to assist in this too. Andrea Stritmatter, HTL(ASCP) MDSPS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, February 18, 2008 12:30 PM To: Douglas D Deltour; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Pregnancy Importance: Low Douglas: All chemicals used in the lab have TWA values LOWER for pregnant women and infants, and that is the reason why pregnant women should not work in the lab environment, regardles it may have TWA levels for adults within the limits. Ren? J. Douglas D Deltour wrote: No not me.. but. What are the regulations/guidelines for working in a histology lab while one is pregnant? Is this an individual choice or a doctor choice? Are there any liabilities if something happens? Thanks. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Feb 19 09:42:23 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 19 09:42:28 2008 Subject: [Histonet] Pregnancy In-Reply-To: Message-ID: <725892.7527.qm@web61224.mail.yahoo.com> I never paid attention to the "discriminating" claim because if the pregnant woman is assigned tasks away from dangerous chemicals WITHOUT affecting her salary, I don't see any discrimination. Granted that thre allways be some "colleague" that could complaint because of some extra work load caused by that change in tasks, but in any event that is something that will last 9 months only and life is too sacred, too precious, to put is at risk for any sort of petty arguments. That is how I see it and how I always did it Ren? J. Robyn Vazquez wrote: I wish this precautions were taken with me when I worked in Portland, OR I had a problem pregnancy and still required to change the Tissue Tek chemicals, because "it wasn't fair". I lost my baby at 5 ? months. And was devastated, but still had to be back to work the next day, as not to put anyone out as to pick up my load. Plus there is more, but won't go into it. Needless to say, my manager NEVER stepped in to help me. Some managers need to be more proactive with their pregnant employees, regardless if is discriminating, use common sense. >>> "Stritmatter, Andrea" 2/18/2008 12:46 PM >>> As a new mother of a 7 month old son and a manager of a histology lab, you have to work with the pregnant individual on this. It is discrimination (at least in Washington state) if forbid them to work in the lab. I chose not to take x-rays or handle DAB during my pregnancy. Otherwise I worked normally in the lab. I ended up with pregnancy complications and couldn't stand all day so I did a lot of paperwork catch up too. Ultimately, it's up to the manager and the employee to work together to find duties you all agree on. Your HR department should be able to assist in this too. Andrea Stritmatter, HTL(ASCP) MDSPS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, February 18, 2008 12:30 PM To: Douglas D Deltour; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Pregnancy Importance: Low Douglas: All chemicals used in the lab have TWA values LOWER for pregnant women and infants, and that is the reason why pregnant women should not work in the lab environment, regardles it may have TWA levels for adults within the limits. Ren? J. Douglas D Deltour wrote: No not me.. but. What are the regulations/guidelines for working in a histology lab while one is pregnant? Is this an individual choice or a doctor choice? Are there any liabilities if something happens? Thanks. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From b-frederick <@t> northwestern.edu Tue Feb 19 09:51:37 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue Feb 19 09:51:56 2008 Subject: [Histonet] Pregnancy In-Reply-To: Message-ID: <006f01c8730f$52f17a40$d00f7ca5@lurie.northwestern.edu> Not having kids,I can still sympathize as I have seen many a pregnant resident. They could fortunately rearrange their schedules to be off anatomic path rotation at the time. I tend to avoid the processors when I have bronchitis as xylene drops my voice into the baritone range and make sure everyone knows it. For you pregnant women, stay away fro chemlawn too. If it can cause lymphoma in dogs as well as miscarriages (this is not firsthand but from working in the gross room and seeing patient history)I'd have loved to have called the person,but that would have been my job. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robyn Vazquez Sent: Tuesday, February 19, 2008 9:13 AM To: histonet@lists.utsouthwestern.edu; andrea.stritmatter@mdsinc.com; doug@ppspath.com; rjbuesa@yahoo.com Subject: RE: [Histonet] Pregnancy I wish this precautions were taken with me when I worked in Portland, OR I had a problem pregnancy and still required to change the Tissue Tek chemicals, because "it wasn't fair". I lost my baby at 5 ? months. And was devastated, but still had to be back to work the next day, as not to put anyone out as to pick up my load. Plus there is more, but won't go into it. Needless to say, my manager NEVER stepped in to help me. Some managers need to be more proactive with their pregnant employees, regardless if is discriminating, use common sense. >>> "Stritmatter, Andrea" 2/18/2008 12:46 PM >>> As a new mother of a 7 month old son and a manager of a histology lab, you have to work with the pregnant individual on this. It is discrimination (at least in Washington state) if forbid them to work in the lab. I chose not to take x-rays or handle DAB during my pregnancy. Otherwise I worked normally in the lab. I ended up with pregnancy complications and couldn't stand all day so I did a lot of paperwork catch up too. Ultimately, it's up to the manager and the employee to work together to find duties you all agree on. Your HR department should be able to assist in this too. Andrea Stritmatter, HTL(ASCP) MDSPS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, February 18, 2008 12:30 PM To: Douglas D Deltour; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Pregnancy Importance: Low Douglas: All chemicals used in the lab have TWA values LOWER for pregnant women and infants, and that is the reason why pregnant women should not work in the lab environment, regardles it may have TWA levels for adults within the limits. Ren? J. Douglas D Deltour wrote: No not me.. but. What are the regulations/guidelines for working in a histology lab while one is pregnant? Is this an individual choice or a doctor choice? Are there any liabilities if something happens? Thanks. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Tue Feb 19 10:03:59 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Feb 19 10:04:07 2008 Subject: AW: [Histonet] Sudan III In-Reply-To: Message-ID: <003701c87311$0a4bd7c0$eeeea8c0@dielangs.at> We prepare our Sudan III solution in Aceton/70% ethanol 1:1 and add as much dye until saturation. Let stand for a few days, filter before use. Allways close tightly because of the aceton-vapor. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Sonny Duong Gesendet: Montag, 18. Februar 2008 18:01 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Sudan III Hi all! I am attempting to perform fecal fat microscopy with Sudan III stain. My protocol requires me to have 1% Sudan III dye, and my question is, 1% in what? The dye is insoluble in water, so what should I dissolve the stain in? Ehtanol? Chloroform?? Thanks, Sonny Duong Carla Green Lab University of Virginia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CBark <@t> memorialcare.org Tue Feb 19 10:05:33 2008 From: CBark <@t> memorialcare.org (Christine Bark) Date: Tue Feb 19 10:06:09 2008 Subject: [Histonet] (no subject) Message-ID: Good Morning, I was hoping I could get some help tracking down some information. I need to find a published article about workload time studies in histology, especially pertaining to embedding and cutting. I thought that CAP published something along these lines but I couldn't find anything on their website. If anyone can point me in the right direction, it would be much appreciated. Thank you in advance for all your help. Christine Bark HT(ASCP) CM Senior Histotech, Pathology Saddleback Memorial Medical Center 949-452-3548 cbark@memorialcare.org ______________________________________________________________________________ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From PMcArdle <@t> ebsciences.com Tue Feb 19 10:06:57 2008 From: PMcArdle <@t> ebsciences.com (Phil McArdle) Date: Tue Feb 19 10:07:04 2008 Subject: [Histonet] re: re: pregnancy Message-ID: <47BAFEA1.6080500@ebsciences.com> Hi - Though I'm no attorney, employment law is pretty clear on this issue: as an employer, you must make all employees aware of hazards and make recommendations, but you cannot discriminate. For example, an auto-parts manufacturer prohibited pregnant workers from working with lead, re-assigning them (at same pay, etc.). An employee filed a discrimination suit, and won. Bottom line - the employer, in what appears to be a good-faith effort to do the responsible thing, was penalized. So my take on this issue is, in addition to making employees aware of hazards, you can make accommodations at the request of the employee, but you cannot force them to change duties without danger of a lawsuit. Phil McArdle -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com I skate to where the puck is going to be, not to where it's been. - Wayne Gretsky You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway. So please do the right thing and make this e-mail go away. Thank you. From gu.lang <@t> gmx.at Tue Feb 19 10:21:44 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Feb 19 10:21:52 2008 Subject: AW: [Histonet] Pregnancy In-Reply-To: <20080218172857.17151gmx1@mx081.gmx.net> Message-ID: <003801c87313$84f43970$eeeea8c0@dielangs.at> There are a few points, that a pregnant woman has to avoid. For example in the DNA-probes for FISH the teratogen Formamid is a part of it. Also the stuff, that is probably cancerogen is not a safe inviroment. I think of xylen, benzol, DAB and also formaldehyd. In our lab pregnant histotechs are not allowed to get in touch with fresh specimen. -- no grossing, no frozen cuts. I think the danger of a hepatitis or anti-viral-therapy is to big to take the risk. I have the opinion, that making the workplace save for a pregnant colleage has nothing to do with discrimination! I am sure there is allways a place, where someone can put a microtome and a waterbath, without any danger. Regardless of any legal regulations it is a matter of personal integrity. If anybody wants to take the risk for her baby, it's her thing - but the manager has to give the possibility of choice. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Douglas D Deltour Gesendet: Montag, 18. Februar 2008 18:23 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Pregnancy No not me.. but. What are the regulations/guidelines for working in a histology lab while one is pregnant? Is this an individual choice or a doctor choice? Are there any liabilities if something happens? Thanks. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcline <@t> wchsys.org Tue Feb 19 10:26:11 2008 From: jcline <@t> wchsys.org (Joyce Cline) Date: Tue Feb 19 10:26:21 2008 Subject: [Histonet] Disposal of paraffin blocks Message-ID: <002601c87314$23f24800$1d2a14ac@wchsys.org> How are clinical labs disposing of their paraffin blocks? Regular trash, incinerated trash or some medical waste company? We have several years to get rid of and our boiler room won't let me send them down to be incinerated. ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From JEllin <@t> yumaregional.org Tue Feb 19 10:28:59 2008 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Tue Feb 19 10:29:24 2008 Subject: [Histonet] Lean Histology In-Reply-To: <003801c87313$84f43970$eeeea8c0@dielangs.at> Message-ID: <29BE166A2CF48D459853F8EC57CD37E8011D875B@EXCHANGECLUSTER.yumaregional.local> Was wondering if anyone can share with me experiences were your lab has gone through the LEAN process,,, shots coming, pit fall,, road blocks, or even improvements. Currently our organization is under going a culture change, as we are seen through out health care and we are being asked (TOLD) to embrance theses concepts,, been through lean trainging for the clinical lab, but really see histology as a different animal. So wanting to get some adive on this portion of the lab. Jesus Ellin HT/PA ASCP Yuma Regional Medical Center This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. From phass <@t> ethz.ch Tue Feb 19 10:32:18 2008 From: phass <@t> ethz.ch (Hass Philipp) Date: Tue Feb 19 10:32:26 2008 Subject: [Histonet] (no subject) Message-ID: Hello, We are doing some investigations on glue-lines in wood. Our standard procedure so far is to boil the wood in water to soften it, produce microtome slices and stain them. However the boiling process might affect the condition of the adhesive and the wood itself. To avoid this effect, we want to survey little wooden cubes (untreated), where the investigated surface has only been smoothed with the microtome. To get a better contrast between the wood and the adhesive, we tried various staining methods (safranine, iodide-iodide-potassium, toluidine-blue), that have been mentioned in the literature. Anyway, the tests results were not satisfying. Not every part of the adhesive was dyed by the staining agent to the same degree making a determination of the adhesive penetration very difficult. My question now is: Has anybody some experience with a dry sample preparation of wood or glue lines respectively? And has anybody some experience with the staining of wood or adhesive? The wood is beech; the adhesives are PUR, UF and PVAC. Thanks, Philipp Philipp Ha? Institute for Building Materials (Wood Physics) ETH Zurich, HIF E 23.2 Schafmattstrasse 6 CH-8093 Zurich Tel: (0041)-(0)44-632 32 50 Fax: (0041)-(0)44/632 11 74 phass@ethz.ch From arsenn <@t> hsh.org Tue Feb 19 10:34:11 2008 From: arsenn <@t> hsh.org (Senn, Amy R) Date: Tue Feb 19 10:34:16 2008 Subject: [Histonet] Calretinin Message-ID: Hi Histoland.... We are having some problems with our Calret stains. Our patient tissue keeps floating off & we are not sure what we're doing wrong (if anything!) We do use charged slides. We float the tissue out in a separate water bath with pure DI water. We allow the slides to stand upright for 30 minutes to air dry, and then put them in a 62 degree oven for another 30 minutes. We are careful not to over-handle the slides at anytime, and we make sure our hands are clean and lotion free. Sometimes, for weeks and weeks, the tissue is right where we put it when it comes off the stainer. Other times, for weeks and weeks, it's gone. And it's only the patient--the control is where's it's supposed to be. Is there something we're missing? Something else we can try?? Thanks so much for all your help!! Amy S. Histotech, Histology Laboratory Holy Spirit Hospital 503 N. 21st Street Camp Hill, PA 17011 (717) 763-2124 Cell: (724) 494-2237 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You From Janet.Bonner <@t> FLHOSP.ORG Tue Feb 19 10:36:25 2008 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Tue Feb 19 10:37:44 2008 Subject: [Histonet] Disposal of paraffin blocks References: <002601c87314$23f24800$1d2a14ac@wchsys.org> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2520@fhosxchmb006.ADVENTISTCORP.NET> We bag them in red biohazard bags and engage a company that specializes in Biohazard waste to dispose of them. Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Joyce Cline Sent: Tue 2/19/2008 11:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Disposal of paraffin blocks How are clinical labs disposing of their paraffin blocks? Regular trash, incinerated trash or some medical waste company? We have several years to get rid of and our boiler room won't let me send them down to be incinerated. ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From oshel1pe <@t> cmich.edu Tue Feb 19 10:42:39 2008 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Tue Feb 19 10:43:09 2008 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: Philipp, This is a good question for the microscopy mailing list: http://www.msa.microscopy.org/MicroscopyListserver/HomePage.html There are people there who work on wood and adhesives -- probably someone who does both. Phil >Hello, > >We are doing some investigations on glue-lines >in wood. Our standard procedure so far is to >boil the wood in water to soften it, produce >microtome slices and stain them. However the >boiling process might affect the condition of >the adhesive and the wood itself. To avoid this >effect, we want to survey little wooden cubes >(untreated), where the investigated surface has >only been smoothed with the microtome. To get a >better contrast between the wood and the >adhesive, we tried various staining methods >(safranine, iodide-iodide-potassium, >toluidine-blue), that have been mentioned in the >literature. Anyway, the tests results were not >satisfying. Not every part of the adhesive was >dyed by the staining agent to the same degree >making a determination of the adhesive >penetration very difficult. > >My question now is: Has anybody some experience >with a dry sample preparation of wood or glue >lines respectively? And has anybody some >experience with the staining of wood or adhesive? > >The wood is beech; the adhesives are PUR, UF and PVAC. > >Thanks, > >Philipp > >Philipp Ha? >Institute for Building Materials (Wood Physics) >ETH Zurich, HIF E 23.2 >Schafmattstrasse 6 >CH-8093 Zurich >Tel: (0041)-(0)44-632 32 50 >Fax: (0041)-(0)44/632 11 74 >phass@ethz.ch >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576 From laurie.colbert <@t> huntingtonhospital.com Tue Feb 19 10:55:30 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Tue Feb 19 10:55:38 2008 Subject: [Histonet] Now what? Message-ID: <57BE698966D5C54EAE8612E8941D7683027B0BAE@EXCHANGE3.huntingtonhospital.com> Oh, Steve, I know exactly how you feel! I have been going through the same thing, and I don't have an answer for you. I also don't know how to get across to everyone that not everyone works at the same pace (and this is not an excuse for the under-achievers to continue under-achieving), and that they just need to do whatever they see that needs doing! I would appreciate hearing any responses that you receive. Laurie Colbert -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of CrochiereSteve@aol.com Sent: Monday, February 18, 2008 3:44 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Now what? I recently began keeping track of workload units in my lab in an attempt to boost the productivity of the staff. In the past I or another tech would do approximately one half of the workload, while the other three techs would pick up the remainder between them. Since I started keeping track of who did what, the underperformers workload has increased and mine has dropped to half of what it had previously been. Sounds great, right? Well, the plan has backfired a little when the three low performers ganged up on my best tech and accused her of monopolizing the work so that they couldn't keep up. I would have expected the opposite, where they would push the lowest ranked tech into pulling their weight. This is not used in any evaluations or merit based bonus. It was just a social experiment on my part. Now my question is: What do I do to smooth out the ruffled feathers of all involved, but keep the work flowing? If I need to dole out equal portions to everyone, production will be delayed and quality will go down. If I allow those who can do more to do so, morale will suffer. Any suggestions? Steven M. Crochiere, HT(ASCP) Histology Supervisor LifePath Partners @ Mercy Medical Center Springfield, MA 01104 **************Ideas to please picky eaters. Watch video on AOL Living. (http://living.aol.com/video/how-to-please-your-picky-eater/rachel-campo s-duffy/ 2050827?NCID=aolcmp00300000002598) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Feb 19 10:57:00 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 19 10:57:05 2008 Subject: [Histonet] (no subject) In-Reply-To: Message-ID: <451418.56102.qm@web61213.mail.yahoo.com> Christine: Under separate cover I am sending an article I wrote on the subject. Ren? J. Christine Bark wrote: Good Morning, I was hoping I could get some help tracking down some information. I need to find a published article about workload time studies in histology, especially pertaining to embedding and cutting. I thought that CAP published something along these lines but I couldn't find anything on their website. If anyone can point me in the right direction, it would be much appreciated. Thank you in advance for all your help. Christine Bark HT(ASCP) CM Senior Histotech, Pathology Saddleback Memorial Medical Center 949-452-3548 cbark@memorialcare.org ______________________________________________________________________________ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From laurie.colbert <@t> huntingtonhospital.com Tue Feb 19 10:58:52 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Tue Feb 19 10:58:56 2008 Subject: [Histonet] Now what? Message-ID: <57BE698966D5C54EAE8612E8941D7683027B0BB1@EXCHANGE3.huntingtonhospital.com> How would you handle this if everyone comes in at different times? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, February 19, 2008 5:55 AM To: CrochiereSteve@aol.com; histonet@pathology.swmed.edu Subject: Re: [Histonet] Now what? Divide the workload equally amongst all and keep track of finishing time and quality. The"excess time" (time left after everything is finished by all) use it for training in special procedures and tasks arounf the lab. You will have shortened the TAT for the pathologists and hopefully everybody will be happy. And you will have also increased productivity since the tasks will be completed in less time. Ren? J. CrochiereSteve@aol.com wrote: I recently began keeping track of workload units in my lab in an attempt to boost the productivity of the staff. In the past I or another tech would do approximately one half of the workload, while the other three techs would pick up the remainder between them. Since I started keeping track of who did what, the underperformers workload has increased and mine has dropped to half of what it had previously been. Sounds great, right? Well, the plan has backfired a little when the three low performers ganged up on my best tech and accused her of monopolizing the work so that they couldn't keep up. I would have expected the opposite, where they would push the lowest ranked tech into pulling their weight. This is not used in any evaluations or merit based bonus. It was just a social experiment on my part. Now my question is: What do I do to smooth out the ruffled feathers of all involved, but keep the work flowing? If I need to dole out equal portions to everyone, production will be delayed and quality will go down. If I allow those who can do more to do so, morale will suffer. Any suggestions? Steven M. Crochiere, HT(ASCP) Histology Supervisor LifePath Partners @ Mercy Medical Center Springfield, MA 01104 **************Ideas to please picky eaters. Watch video on AOL Living. (http://living.aol.com/video/how-to-please-your-picky-eater/rachel-campos-duffy/ 2050827?NCID=aolcmp00300000002598) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Feb 19 11:02:06 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 19 11:02:13 2008 Subject: [Histonet] Now what? In-Reply-To: <57BE698966D5C54EAE8612E8941D7683027B0BB1@EXCHANGE3.huntingtonhospital.com> Message-ID: <620353.24445.qm@web61216.mail.yahoo.com> Those arriving first will take care of the rushes, the others cases will be shared and if you have autopsy blocks those will be for those arriving later. The thing is that everybody gets their equal share of work. Ren? J. Laurie Colbert wrote: How would you handle this if everyone comes in at different times? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, February 19, 2008 5:55 AM To: CrochiereSteve@aol.com; histonet@pathology.swmed.edu Subject: Re: [Histonet] Now what? Divide the workload equally amongst all and keep track of finishing time and quality. The"excess time" (time left after everything is finished by all) use it for training in special procedures and tasks arounf the lab. You will have shortened the TAT for the pathologists and hopefully everybody will be happy. And you will have also increased productivity since the tasks will be completed in less time. Ren? J. CrochiereSteve@aol.com wrote: I recently began keeping track of workload units in my lab in an attempt to boost the productivity of the staff. In the past I or another tech would do approximately one half of the workload, while the other three techs would pick up the remainder between them. Since I started keeping track of who did what, the underperformers workload has increased and mine has dropped to half of what it had previously been. Sounds great, right? Well, the plan has backfired a little when the three low performers ganged up on my best tech and accused her of monopolizing the work so that they couldn't keep up. I would have expected the opposite, where they would push the lowest ranked tech into pulling their weight. This is not used in any evaluations or merit based bonus. It was just a social experiment on my part. Now my question is: What do I do to smooth out the ruffled feathers of all involved, but keep the work flowing? If I need to dole out equal portions to everyone, production will be delayed and quality will go down. If I allow those who can do more to do so, morale will suffer. Any suggestions? Steven M. Crochiere, HT(ASCP) Histology Supervisor LifePath Partners @ Mercy Medical Center Springfield, MA 01104 **************Ideas to please picky eaters. Watch video on AOL Living. (http://living.aol.com/video/how-to-please-your-picky-eater/rachel-campos-duffy/ 2050827?NCID=aolcmp00300000002598) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From rjbuesa <@t> yahoo.com Tue Feb 19 11:02:44 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 19 11:02:48 2008 Subject: [Histonet] Disposal of paraffin blocks In-Reply-To: <002601c87314$23f24800$1d2a14ac@wchsys.org> Message-ID: <891593.28777.qm@web61214.mail.yahoo.com> I always used the incinerated trash option. Ren? J. Joyce Cline wrote: How are clinical labs disposing of their paraffin blocks? Regular trash, incinerated trash or some medical waste company? We have several years to get rid of and our boiler room won't let me send them down to be incinerated. ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From histology <@t> gradymem.org Tue Feb 19 11:06:01 2008 From: histology <@t> gradymem.org (histology@gradymem.org) Date: Tue Feb 19 11:06:14 2008 Subject: [Histonet] Disposal of paraffin blocks In-Reply-To: <002601c87314$23f24800$1d2a14ac@wchsys.org> References: <002601c87314$23f24800$1d2a14ac@wchsys.org> Message-ID: We used to trash our paraffin blocks but now we have to dispose of them as infectious waste. Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org ----- Original Message ----- From: Joyce Cline Date: Tuesday, February 19, 2008 10:35 am Subject: [Histonet] Disposal of paraffin blocks To: histonet@lists.utsouthwestern.edu > How are clinical labs disposing of their paraffin blocks? Regular > trash,incinerated trash or some medical waste company? We have > several years > to get rid of and our boiler room won't let me send them down to be > incinerated. > > > ***** CONFIDENTIALITY NOTICE ***** > This message contains confidential information and is intended > only for > the individual named. If you are not the named addressee you > should not > disseminate, distribute or copy this e-mail. Please notify the sender > immediately by e-mail if you have received this e-mail by mistake and > delete this e-mail from your system. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Rcartun <@t> harthosp.org Tue Feb 19 11:22:14 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Feb 19 11:22:28 2008 Subject: [Histonet] t(12;15) Test Message-ID: <47BAC9F6020000770000ACED@gwmail4.harthosp.org> Is anyone doing consultative testing for the t(12;15) translocation associated with Congenital Infantile Fibrosarcoma? Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From jmahoney <@t> alegent.org Tue Feb 19 11:23:59 2008 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Tue Feb 19 11:24:21 2008 Subject: [Histonet] Lean Histology In-Reply-To: <29BE166A2CF48D459853F8EC57CD37E8011D875B@EXCHANGECLUSTER.yumaregional.local> References: <003801c87313$84f43970$eeeea8c0@dielangs.at> <29BE166A2CF48D459853F8EC57CD37E8011D875B@EXCHANGECLUSTER.yumaregional.local> Message-ID: <47BABC4F0200003C0002BED2@gwia.alegent.org> Jesus, We have implemented LEAN in my laboratory and have seen remarkable gains. I will be speaking at the New Mexico regional National Meeting in June about our experience. I hope you can attend. We cut TAT, reduced space, reduced cost and continue to make improvements. AP is different but the principles can be applied anywhere. Jan Mahoney Alegent Health Omaha, NE Janice Mahoney Histology/Cytology Coordinator Alegent Health Laboratory 4955 F Street Omaha, NE 68117 (402)717-2889 >>> Jesus Ellin 02/19/2008 10:28 AM >>> Was wondering if anyone can share with me experiences were your lab has gone through the LEAN process,,, shots coming, pit fall,, road blocks, or even improvements. Currently our organization is under going a culture change, as we are seen through out health care and we are being asked (TOLD) to embrance theses concepts,, been through lean trainging for the clinical lab, but really see histology as a different animal. So wanting to get some adive on this portion of the lab. Jesus Ellin HT/PA ASCP Yuma Regional Medical Center From laurie <@t> conxis.com Tue Feb 19 11:43:23 2008 From: laurie <@t> conxis.com (Laurie Popp) Date: Tue Feb 19 11:43:36 2008 Subject: [Histonet] Re: Pregnancy Message-ID: <47BB153B.6010001@conxis.com> Hi all... Happy Tuesday Pregnancy seems to be in the water in our lab, I have a happy ( although not so happy today due to teething) 6 month old, our QC person just delivered on Valentine's day, our histology instructor delivered the week after new year's, and we have 3 more pregnant currently within our lab. We also have been in the process of remodeling our space and moving into new areas. All of our techs do everything within the lab from specials all the way through to grossing and right now because of some design flaws the pregnant techs are restricted from grossing due to ventilation issues with the new downdraft tables in gross cutting. When I was pregnant I let my physician know that I was working in histology and what chemicals I was exposed to. I tried to limit my exposure to formalin, B5 ( we use this on our bone marrows), and some of the different staining solutions ( zenker's and so on) and when I did have to work in those areas I made sure to follow universal precautions and wore appropriate PPE. One of the docs that I was working with has a sub specialty in chemical exposures during pregnancy and told me that this would be acceptable as I was a student during my pregnancy so I had to rotate through all areas of the lab prior to completing my course. ( I should also add I was high risk but it would behoove anyone to communicate workplace information with their OB/GYN when they first find out they are expecting). I spent a lot of time cutting and embedding :-) Laurie Popp HT ( ASCP) Mayo Clinic From settembr <@t> umdnj.edu Tue Feb 19 11:49:29 2008 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Tue Feb 19 11:50:10 2008 Subject: [Histonet] Calretinin Message-ID: Are you putting the patient section on the same slide as the positive control? If you are, and you have precut, preheated positive control slides waiting to be used, there's a posibility that the patient section may be placed on top of some melted paraffin coming off the pos. cont., thus not giving the pt. case much to hang on to. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> "Senn, Amy R" 02/19/08 11:34 AM >>> Hi Histoland.... We are having some problems with our Calret stains. Our patient tissue keeps floating off & we are not sure what we're doing wrong (if anything!) We do use charged slides. We float the tissue out in a separate water bath with pure DI water. We allow the slides to stand upright for 30 minutes to air dry, and then put them in a 62 degree oven for another 30 minutes. We are careful not to over-handle the slides at anytime, and we make sure our hands are clean and lotion free. Sometimes, for weeks and weeks, the tissue is right where we put it when it comes off the stainer. Other times, for weeks and weeks, it's gone. And it's only the patient--the control is where's it's supposed to be. Is there something we're missing? Something else we can try?? Thanks so much for all your help!! Amy S. Histotech, Histology Laboratory Holy Spirit Hospital 503 N. 21st Street Camp Hill, PA 17011 (717) 763-2124 Cell: (724) 494-2237 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mthomas <@t> littonlab.com Tue Feb 19 11:50:33 2008 From: mthomas <@t> littonlab.com (Marla Thomas) Date: Tue Feb 19 11:50:50 2008 Subject: [Histonet] re: pregnancy Message-ID: <004601c8731f$ed0e7ff0$9d35a8c0@LittonPath.local> In the state of Missouri it is also considered discrimination if the employer refuses to allow an employee to perform tasks in their job description because they are pregnant. Having said that, we council the employee on chemicals that are hazardous, and we have a great group of techs who will go out of their way to insure that all of the jobs get done without the pregnant tech having to put her or her unborn child at risk. We also monitor air flow and quality, formalin and xylene exposure to ensure all of our employees work in an environment that is as safe as it can be (I am sure all of your labs do this also). Any employee who is concerned about exposure can access the results from our testing ( we test all of the high risk tasks). To wrap this up if the tech want to continue their tasks we cannot tell them no, but if there are tasks they are uncomfortable doing, someone else will do them. Marla Thomas, HT(ASCP) HIPAA/Compliance/Safety Officer/IT Manager Litton Pathology Associates, PC 700 NW Hunter Drive Blue Springs, MO 64015 816-229-6449, fax 816-874-4400 This e-mail, including attachments, may include confidential and/or proprietary information, and may be used only by the person or entity to which it is addressed. If the reader of this e-mail is not the intended recipient or his/her authorized agent, the reader is hereby notified that any dissemination, distribution or copying of this e-mail is prohibited. If you have received this e-mail in error, please notify the sender by replying to this message and delete this e-mail immediately. From tkngflght <@t> yahoo.com Tue Feb 19 12:16:11 2008 From: tkngflght <@t> yahoo.com (Cheryl) Date: Tue Feb 19 12:16:39 2008 Subject: Balance and common sense RE: [Histonet] re: re: pregnancy In-Reply-To: <47BAFEA1.6080500@ebsciences.com> References: <47BAFEA1.6080500@ebsciences.com> Message-ID: <007b01c87323$82697ca0$6601a8c0@FSROGER> BUT---if the pregnant woman enlists the help of her care physician and he makes recommendations for duty alterations because of a health condition And pregnancy IS a condition--it's not a normal state of being (FMLA-governed, too). I understand and have been on both sides of this issue. My daughter was born over a month early because of effects of working in a lab and I finished the CAP inspection from my hospital bed with my kid in an incubator--and yes I still think of that facility as one of the best I ever worked. We had several uneventful pregnancies while I was a part of that lab and several other labs, too. NO ONE wants to cause harm because they are afraid of a lawsuit. C'mon guys, were talking about a baby. Work with the mom, work with her physician, educate everyone, use your risk management department and employee health department!!!! If your facility is of any size and especially if there is a nursing staff, this is not a new situation to your facility. If you offer her more safety equipment--offer it to all. Please let's not lose sight of the fact that labs are run and operate on the efforts of people who have families and lives outside of work. Take care of your people, stay inside the rules but do so creatively and use your resources--mom, baby, lab and all those who work there will finish this experience healthy, happy and gainfully producing good patient care. Cheryl Cheryl R. Kerry, HT(ASCP), BA Full Staff Inc. Staffing the lab - One GREAT tech at a time. 281.852.9457 office 281.883.7704 cell 800.756.3309 fax and alternate phone admin@fullstaff.org www.fullstaff.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Phil McArdle Sent: Tuesday, February 19, 2008 10:07 AM To: Histonet Subject: [Histonet] re: re: pregnancy Hi - Though I'm no attorney, employment law is pretty clear on this issue: as an employer, you must make all employees aware of hazards and make recommendations, but you cannot discriminate. For example, an auto-parts manufacturer prohibited pregnant workers from working with lead, re-assigning them (at same pay, etc.). An employee filed a discrimination suit, and won. Bottom line - the employer, in what appears to be a good-faith effort to do the responsible thing, was penalized. So my take on this issue is, in addition to making employees aware of hazards, you can make accommodations at the request of the employee, but you cannot force them to change duties without danger of a lawsuit. Phil McArdle -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Mobile: 860.597.6796 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com I skate to where the puck is going to be, not to where it's been. - Wayne Gretsky You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, however, there's not a lot I can do about it, and it was probably my mistake anyway. So please do the right thing and make this e-mail go away. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pkarlisch <@t> hmc.psu.edu Tue Feb 19 13:07:14 2008 From: pkarlisch <@t> hmc.psu.edu (Patricia Karlisch) Date: Tue Feb 19 13:07:32 2008 Subject: [Histonet] Grocott Stain - quick method Message-ID: <47BAE293.07B7.008C.0@hmc.psu.edu> Histonetters, Does anyone have a quick method to do the Grocott stain. Thank you, Pat Karlisch pkarlisch@psu.edu Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. From Lori.Disher <@t> HCAhealthcare.com Tue Feb 19 13:17:47 2008 From: Lori.Disher <@t> HCAhealthcare.com (Disher Lori) Date: Tue Feb 19 13:18:11 2008 Subject: [Histonet] Slide storage Message-ID: <095327C7CDBDF64B9E9728A54799091E33A5D8@ORLEV03.hca.corpad.net> Is ok to store H & E, Pap, immuno, special stained slides in a 55 degree (F) room with no more than 50% humidity? We have had problems with slides fading in the past. From lhunt <@t> uta.edu Tue Feb 19 13:34:53 2008 From: lhunt <@t> uta.edu (Laura Hunt) Date: Tue Feb 19 13:34:57 2008 Subject: [Histonet] ionic liquids as preservation mediums In-Reply-To: References: Message-ID: <1B50538E-725B-4623-BF79-66FF29B4657A@uta.edu> Hello Histonetters: I have another unusual question. I have a friend in chemistry who has made a bunch of ionic liquids and is looking for potential applications. For example, as a preservation medium for tissues, instead of formalin. Anybody know if this is worth pursuing? If so, would anyone be interested in a collaboration with him? Properties: 1. Made out of tartaric acid 2. Non-toxic 3. Cheaply made on a benchtop Thanks, LH From dcrippen <@t> buckinstitute.org Tue Feb 19 13:40:30 2008 From: dcrippen <@t> buckinstitute.org (Danielle Crippen) Date: Tue Feb 19 13:40:45 2008 Subject: [Histonet] PFA degradation Message-ID: Hi Histo-experts, When I first came to my department 5 years ago, it was common practice to make up 8% PFA (from prills) and store it in the fridge until it needed to be diluted and used. But, recently, the concern has been raised about degradation... My question is...how do others commonly deal with PFA?? Do you make it fresh each time you use it?? If so, what is the favorite protocol?? And, just out of curiosity, has anyone ever done an analysis of stored PFA to see how fast it breaks down...or how long it can safely be stored and used?? Many thanks in advance!! Cheers, Danielle From rjbuesa <@t> yahoo.com Tue Feb 19 13:54:48 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 19 13:54:57 2008 Subject: [Histonet] Lean Histology In-Reply-To: <29BE166A2CF48D459853F8EC57CD37E8011D875B@EXCHANGECLUSTER.yumaregional.local> Message-ID: <56289.96877.qm@web61211.mail.yahoo.com> As you very well said "histology is a different animal". It is not that "common sense" changes can be done under the "lean umbrella" like higher efficiency at every step, but the real "unitary or quasi unitary" workflow is out of the question. The ones that have approached closest to the implementation of Lean in histology is Sakura with their Xpress and it has been done will small batches (of 40 cassettes the most) but batching cannot be eliminated. All in all Lean isn't suitable for processes that require a high degree of craftmanship, as histology does (remember the step called secioning?). Artisanship takes time, and any creative process trades inefficiency for creative results, and that is the realm of histology. Sakura has an automated embedding center and Kurado company has developed an instrument (the Kurado AS200) to section, but only specimens the are standard and for multiple sections, like controls. We are a long way from Lean, we are closer to Six Sigma and all its controls and QA steps. Ren? J. Jesus Ellin wrote: Was wondering if anyone can share with me experiences were your lab has gone through the LEAN process,,, shots coming, pit fall,, road blocks, or even improvements. Currently our organization is under going a culture change, as we are seen through out health care and we are being asked (TOLD) to embrance theses concepts,, been through lean trainging for the clinical lab, but really see histology as a different animal. So wanting to get some adive on this portion of the lab. Jesus Ellin HT/PA ASCP Yuma Regional Medical Center This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From rjbuesa <@t> yahoo.com Tue Feb 19 13:56:44 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 19 13:56:48 2008 Subject: [Histonet] Calretinin In-Reply-To: Message-ID: <426259.75395.qm@web61218.mail.yahoo.com> Your problem is not with calretinin but with a poorly infiltrated tissue sectioned for the test. If you can process another piece of the tissue, or add a coat of Elmer's glue on a slide, let dry and use it to pick up new sections of your case. Ren? J. "Senn, Amy R" wrote: Hi Histoland.... We are having some problems with our Calret stains. Our patient tissue keeps floating off & we are not sure what we're doing wrong (if anything!) We do use charged slides. We float the tissue out in a separate water bath with pure DI water. We allow the slides to stand upright for 30 minutes to air dry, and then put them in a 62 degree oven for another 30 minutes. We are careful not to over-handle the slides at anytime, and we make sure our hands are clean and lotion free. Sometimes, for weeks and weeks, the tissue is right where we put it when it comes off the stainer. Other times, for weeks and weeks, it's gone. And it's only the patient--the control is where's it's supposed to be. Is there something we're missing? Something else we can try?? Thanks so much for all your help!! Amy S. Histotech, Histology Laboratory Holy Spirit Hospital 503 N. 21st Street Camp Hill, PA 17011 (717) 763-2124 Cell: (724) 494-2237 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From ASelf <@t> georgetownhospitalsystem.org Tue Feb 19 14:00:47 2008 From: ASelf <@t> georgetownhospitalsystem.org (Amy Self) Date: Tue Feb 19 14:00:53 2008 Subject: [Histonet] Specimen Rejection - QA Message-ID: Hello Histonetters, I was wandering if anyone had any info or some kind of guidelines to help me get started on a QA study especially for specimen rejection. Thanks in advance, Amy Amy Self Georgetown Hospital Systems NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From rjbuesa <@t> yahoo.com Tue Feb 19 14:01:30 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 19 14:01:35 2008 Subject: [Histonet] Slide storage In-Reply-To: <095327C7CDBDF64B9E9728A54799091E33A5D8@ORLEV03.hca.corpad.net> Message-ID: <802518.30817.qm@web61216.mail.yahoo.com> It is very unlikely that either the humidity or a 55?F will cause the fading. That is ussually associated with improperly treated slides, including sections bued in ammonia water and later poorly wahed, or a change in the mounting medium (too acid). Those could be your "culprits" but not temp. or humidity. Ren? J. Disher Lori wrote: Is ok to store H & E, Pap, immuno, special stained slides in a 55 degree (F) room with no more than 50% humidity? We have had problems with slides fading in the past. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From rjbuesa <@t> yahoo.com Tue Feb 19 14:28:50 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 19 14:28:53 2008 Subject: [Histonet] Specimen Rejection - QA In-Reply-To: Message-ID: <189679.66960.qm@web61224.mail.yahoo.com> Before you can even start thinking about specimen rejection you have to define your standards and when the specimens do not comply with your standards, then you can start rejecting them. Ren? J. Amy Self wrote: Hello Histonetters, I was wandering if anyone had any info or some kind of guidelines to help me get started on a QA study especially for specimen rejection. Thanks in advance, Amy Amy Self Georgetown Hospital Systems NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From hlukey <@t> msn.com Tue Feb 19 14:37:13 2008 From: hlukey <@t> msn.com (Hugh Luk) Date: Tue Feb 19 14:37:20 2008 Subject: [Histonet] Disposal of paraffin blocks In-Reply-To: <002601c87314$23f24800$1d2a14ac@wchsys.org> References: <002601c87314$23f24800$1d2a14ac@wchsys.org> Message-ID: Joyce, We checked with our state DOH, and they advised us to burn or "Landfill" the tissue blocks. I clogged one of the hospital boiler room furnaces with wax. My name was "Mudd" for quite a while! The DOH then noticed that every hospital was in the middle of densely populated areas; and shut every single furnace down. As a community, we then made a "Discard Tissue Repository" thru the local university-research group. Our local arrangements are: the research group de-identifies the old blocks and the research group buys new cabinets for the participating hospitals. Yes, this is harder than it sounds, and there is a NCI/SEER grant involved, but as a resource tool, it is outstanding. Hugh Luk Cancer Research Center of Hawaii (but also a hospital employee as a 2nd job) > From: jcline@wchsys.org> To: histonet@lists.utsouthwestern.edu> Date: Tue, 19 Feb 2008 11:26:11 -0500> Subject: [Histonet] Disposal of paraffin blocks> > How are clinical labs disposing of their paraffin blocks? Regular trash,> incinerated trash or some medical waste company? We have several years> to get rid of and our boiler room won't let me send them down to be> incinerated.> > > ***** CONFIDENTIALITY NOTICE *****> This message contains confidential information and is intended only for> the individual named. If you are not the named addressee you should not> disseminate, distribute or copy this e-mail. Please notify the sender> immediately by e-mail if you have received this e-mail by mistake and> delete this e-mail from your system.> > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Helping your favorite cause is as easy as instant messaging.?You IM, we give. http://im.live.com/Messenger/IM/Home/?source=text_hotmail_join From AnthonyH <@t> chw.edu.au Tue Feb 19 14:50:19 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Feb 19 14:51:04 2008 Subject: [Histonet] ionic liquids as preservation mediums Message-ID: There are some articles on the use of ionic liquids as a replacement for formalin eg Pernaka et al (2005) Acta Histochemica 107(2):149-156 In summary: Pernaka et al (2005) from Poland have advocated the use of ionic fluids as replacements for formalin. Ionic liquids (ILs) are a novel class of compounds, and are called solvents of the 21st century. They are molten organic salts, typically comprised of bulky cations and weakly coordinated anions. Some of the better studied ILs contain a heterocyclic cation based on a substituted imidazole or pyridine. They have advantageous chemical and physical properties, such as a negligible vapour pressure, low melting temperature (<100 ?C), a broad liquid range, thermal, chemical and electrochemical stability, favourable solvation behaviour (many organic, organometallic and inorganic compounds can be dissolved in ILs) and have a large electrochemical window. They are also highly polar yet non-coordinating, non-flammable, easy to handle in a variety of standard experimental procedures and they are recyclable. This non-volatile nature means that ILs have been recognized as 'green' solvent alternatives to volatile organic solvents, and it decreases the risk of exposure and loss of solvent by evaporation. Careful selection of cation-anion pairs allows control of several chemical and physical properties of ILs. Moreover, it is possible to fine-tune their miscibility with water by changing the characteristics of side chains in the cation or the type of anion. In recent years, the number of possible cation and anion combinations has increased significantly. Pernaka et al (2005) have categorized ILs, into room temperature ionic liquids (RTILs), protic ILs, task-specific ionic liquids (TSILs) and chiral ILs (Pernaka et al 2005). They found that 1-methyl-3-octyloxymethylimidazolium tetrafluoroborate (IL-[(C8H17OCH2)MIM][BF4]) exhibited tissue fixation properties similar to those of formalin and can be applied in both histological and immunohistochemical techniques. Tissue material fixed with [(C8H17OCH2)MIM][BF4] manifested a more intense staining than those fixed with formalin. With respect to the pattern, distribution and intensity of immunohistochemical staining, it was comparable in tissue material fixed with formalin or [(C8H17OCH2)MIM][BF4]. The time of fixation in formalin or IL had no effect on morphology. Their study confirmed the suitability of 1-methyl-3-octyloxymethylimidazolium tetrafluoroborate as fixative in histopathological procedures, eliminating the necessity of using formalin. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laura Hunt Sent: Wednesday, 20 February 2008 6:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ionic liquids as preservation mediums Hello Histonetters: I have another unusual question. I have a friend in chemistry who has made a bunch of ionic liquids and is looking for potential applications. For example, as a preservation medium for tissues, instead of formalin. Anybody know if this is worth pursuing? If so, would anyone be interested in a collaboration with him? Properties: 1. Made out of tartaric acid 2. Non-toxic 3. Cheaply made on a benchtop Thanks, LH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From tanisha.mcknight <@t> covance.com Tue Feb 19 15:22:18 2008 From: tanisha.mcknight <@t> covance.com (McKnight, Tanisha) Date: Tue Feb 19 15:22:24 2008 Subject: [Histonet] Histology Staining Poster Atlas Message-ID: <816E3C72F855F14985FC31D7C963AE6F049EB295@indexch03.ent.covance.com> Hello All: I am looking for a large color poster that has different examples of stained slides on them. I am hoping this will help some of the people I work with to identify common histology stains. Does anyone have something like this or know where to get one? Tanisha N. McKnight, HT (ASCP) Covance CLS Indianapolis Specimen Management, Anatomic Pathology (317) 271-1200 ext. 7252 ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From histology.bc <@t> shaw.ca Tue Feb 19 15:33:48 2008 From: histology.bc <@t> shaw.ca (Paul Bradbury) Date: Tue Feb 19 15:30:18 2008 Subject: [Histonet] Grocott Stain - quick method In-Reply-To: <47BAE293.07B7.008C.0@hmc.psu.edu> References: <47BAE293.07B7.008C.0@hmc.psu.edu> Message-ID: <47BB4B3C.1020909@shaw.ca> Hi Patricia, A modification that I developed a number of years ago permits the Grocott Silver method to be completed in less than 45 minutes. When I devised this method, I was teaching Histology in Vancouver and the allotted lab periods were only two hours in length, so a number of methods had to be "tweaked" to allow them to be completed by even the slowest students within that time period. This method has actually been adopted by several clinical labs as it is so much quicker, but without sacrificing the specificity. 1. Bring sections to water as usual. 2. Oxidize in 10% chromic acid for 10 minutes (this step is the biggest time saver) (While this is happening, make up the Grocott silver working solution in a very clean glass Coplin jar)) 3. Wash thoroughly in running water 4. Treat with 1% sodium metabisulphite for 1 minute (While this is happening, place the Coplin jar in a 56 degree water bath) 5. Wash well in several changes of distilled water 6. Place the section(s) in the warm silver solution. Use plastic forceps as metal ones will cause the silver to precipitate. 7. Watch the sections carefully, after a couple of minutes they will begin to turn golden-brown. 8. Place the sections in a Coplin jar of distilled water. Examine the control section microscopically to gauge the intensity of the silver impregnation. Ideally, fungi (or protozoa, Pneumocystis, etc) will appear black on a golden background. If the fungi appear too pale, rinse the sections again in distilled water and place them back in the silver solution for a few more seconds. Re-examine the sections, etc. 9. When the fungi appear correctly impregnated, wash thoroughly in distilled water. 10. Treat with gold chloride (whatever concentration you have on hand) 11. Wash. 12. Treat with 1% sodium thiosulphate 13. Wash 14. Dehydrate, Clear and mount Do not be tempted to pre-heat the silver solution to soon. If you do this, or if the Coplin jar is not scrupulously clean, the silver solution will precipitate to form a silver mirror on the glass. Paul Bradbury Kamloops, Canada Patricia Karlisch wrote: > Histonetters, > Does anyone have a quick method to do the Grocott stain. > Thank you, > Pat Karlisch pkarlisch@psu.edu > > Pat Karlisch > Supervisor, Histology, Pathology and Laboratory Medicine > Penn State Milton S. Hershey Medical Center > Mail Code H179 > Hershey, PA 17033 > Phone (717) 531-6072 > Fax: (717) 531- 7741 > email: pkarlisch@psu.edu > > *****E-Mail Confidentiality Notice***** > This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From Tony_Reilly <@t> health.qld.gov.au Tue Feb 19 17:05:11 2008 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Tue Feb 19 17:05:36 2008 Subject: [Histonet] Calretinin In-Reply-To: References: Message-ID: <47BBED46.471C.0039.0@health.qld.gov.au> Hi Amy You are taking all of the right precautions to keep the sections on the slide. If it is an intermittent problem it may be related to the type of tissue being stained. As Calretinin is used for the diagnosis of mesothelioma you may find that it is the cases when pleura or pleural plaques are being stained that are floating off. Pleura by it's nature of being predominantly connective tissue and having little protein content is notorious for floating during IHC. The only suggestions I could make to improve your capture rate are to make sure your sections are absolutely flat on the waterbath to ensure maximum attachment to the slide as pleura like other tougher tissue like cartilage can tend to cut thicker than the wax envelope in the block creating a wave like appearance to the tissue. Secondly try drying your sections overnight at 37C. This will slow down your TAT but beats restaining. Thirdly, if you get really desperate investigate other methods of HIER as this is the step most likely to cause loss of tissue from the slides. There have been a number of papers written on using lower temperatures for longer periods ie 70C for a couple of hours instead of 100C for 20-30 minutes. regards Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:0732402930 tony_reilly@health.qld.gov.au ( mailto:073240tony_reilly@health.qld.gov.au ) >>> "Senn, Amy R" 20/02/2008 2:34 am >>> Hi Histoland.... We are having some problems with our Calret stains. Our patient tissue keeps floating off & we are not sure what we're doing wrong (if anything!) We do use charged slides. We float the tissue out in a separate water bath with pure DI water. We allow the slides to stand upright for 30 minutes to air dry, and then put them in a 62 degree oven for another 30 minutes. We are careful not to over-handle the slides at anytime, and we make sure our hands are clean and lotion free. Sometimes, for weeks and weeks, the tissue is right where we put it when it comes off the stainer. Other times, for weeks and weeks, it's gone. And it's only the patient--the control is where's it's supposed to be. Is there something we're missing? Something else we can try?? Thanks so much for all your help!! Amy S. Histotech, Histology Laboratory Holy Spirit Hospital 503 N. 21st Street Camp Hill, PA 17011 (717) 763-2124 Cell: (724) 494-2237 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/ received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. **************************************************************** From Tony_Reilly <@t> health.qld.gov.au Tue Feb 19 17:20:44 2008 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Tue Feb 19 17:26:51 2008 Subject: [Histonet] Grocott Stain - quick method In-Reply-To: <47BAE293.07B7.008C.0@hmc.psu.edu> References: <47BAE293.07B7.008C.0@hmc.psu.edu> Message-ID: <47BBF0EC.471C.0039.0@health.qld.gov.au> Hi Patricia This is a method that takes approximately 30 minutes that has been used successfully for 10-15 years for urgent biopsies for lung transplant patients. You will need to determine you own times for the microwaving of the silver solution. The important thing is to heat and mix in stages to prevent uneven temperatures within your solution which will result in uneven staining across your section. 1 REAGENTS ANDEQUIPMENT4.1 Reagents 1. 10% Chromic Acid (AQ) Chromium Trioxide - 5g. Distilled water - 50ml. 2. 5% AQ Silver Nitrate Silver Nitrate - 5g. Distilled water - 100ml. 3. 3% AQ Methenamine (Hexamine) Hexamine - 3g. Distilled water - 100ml. 4. 5% AQ Borax (Photographic Grade) Sodium Tetraborate - 5g. Distilled water - 100ml. 5. Stock Methanamine ? Silver nitrate solution 5% Silver Nitrate AQ - 5ml. 3% Methenamine AQ - 100ml. 6. Working Methenamine Silver Nitrate Solution:? Borax 5% AQ - 2ml. Distilled water - 25ml. Mix and add:? Methenamine?Silver Stock Solution - 25ml. 7. 1% Sodium Metabisulphite AQ Sodium Metabisulphite - 1g. Distilled water - 100ml. 8. 0.1% AQ Gold Chloride Gold chloride - 1g. Distilled water - 1000ml. 9. 2% Light Green (Stock) Light green - 2g. Distilled water - 100ml. Acetic Acid - 1ml. 10. Light Green Working Solution Light green stock - 1 part. Distilled water - 19 parts 11. 2% Sodium Thiosulphate Sodium Thiosulphate - 10g Distilled Water - 500ml. 4.2 Equipment Panasonic 1000W Microwave Model NN-5782WF 2 PROCEDURE 1. Bring sections to water 2. Place sections on staining rack and cover with, or place in a coplin jar containing a freshly prepared 10% chromic acid solution for 10minutes. This solution may be stored for one week in the refrigerator. 3. Washthoroughly in running tap water in container in sink 1 minute 4. Rinse in 1% Sodium Metabisulphite 30 seconds 5. Wash thoroughly in tap water for a minimum of 2 minutes. 6. Place slides on rack and rinse well in distilled water before placing in silver solution. 7. Place coplin jar in centre of microwave. Microwave on full power for 20 seconds. Mix solution well with disposable pipette to ensure even distribution of heat. Microwave again on full power for 15 seconds. Remove slides from microwave and mix thoroughly with disposable pipette. Check slides microscopically for end point which should take 3-5 minutes. At the end point fungi should be dark brown to black in colour while background remains golden brown. 8. Carefully tip off solution in coplin jar and replace with distilled water 9. Place slides on rack and give two more changes of distilled water 10. Tone in 0.1% Gold Chloride 2 minutes 11. Rinse in distilled water and flood with 2% aq. sodium thiosulphate ("hypo") 2 minutes (fixing) 12. Rinse in distilled water 13. Counterstain with freshly prepared light green working solution for 20seconds. 14. Washslides briefly in tap water. 14. Dehydrate, clear and mount 3 RESULTS Fungi - Black Background - Green 4 NOTES 1. This method is not specific for fungi but rarely fails to demonstrate any fungi present. Reticulin fibres and threads of fibrin will be blackened by this method if overstaining occurs, and must not be confused with fungi. regards Tony Reilly Chief Scientist Anatomical Pathology Pathology Queensland Princess Alexandra Hospital Ipswich Rd, Woolloongabba Q 4102 Australia Ph: 07 32402412 Fax:0732402930 tony_reilly@health.qld.gov.au ( mailto:073240tony_reilly@health.qld.gov.au ) >>> "Patricia Karlisch" 20/02/2008 5:07 am >>> Histonetters, Does anyone have a quick method to do the Grocott stain. Thank you, Pat Karlisch pkarlisch@psu.edu Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/ received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. **************************************************************** From fudo <@t> ufl.edu Tue Feb 19 17:53:56 2008 From: fudo <@t> ufl.edu (FU,DONGTAO) Date: Tue Feb 19 17:54:01 2008 Subject: [Histonet] quenching of Fluorescent signal Message-ID: <1198258712.219031203465236549.JavaMail.osg@osgjas03.cns.ufl.edu> Hi, all I am doing fluorescent staining on fresh frozen human spleen using CD markers, like CD4, CD8, etc these days. The secondary antibody I used is AF 488, the mount media was DAPI(hard set) from vector. I can get very nice staining, however, I find these signals only can be kept for a couple of days, then start to quench deeply, although I put my stained slides in 4C. Does anyone have experience on how to keep fluorecent signal last longer? And usually how long do you still get your IF signal after mounting? Thank you, Ann Dongtao Fu MD, Ph.D Lab Manager Dept. of Pathology Lab phone: 352-273-7752 Lab FAX: 352-273-7755 Lab address: D11-50 PO Box: 100275 1600 SW Archer Road University of Flodrida Gainesville, FL 32610 From jnocito <@t> satx.rr.com Tue Feb 19 19:36:53 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Feb 19 19:36:56 2008 Subject: [Histonet] Pregnancy References: Message-ID: <005c01c87361$12a982a0$0302a8c0@yourxhtr8hvc4p> this is a case of promoting people to a higher level of incompetence. It fries my cookies that people get promoted to positions that they have no bother getting promoted to. I'm sorry you had to go through that and sorry you had a rectum for a supervisor. I may not be the best manager, but I do what's right by my people. JTT ----- Original Message ----- From: "Robyn Vazquez" To: ; ; ; Sent: Tuesday, February 19, 2008 9:12 AM Subject: RE: [Histonet] Pregnancy I wish this precautions were taken with me when I worked in Portland, OR I had a problem pregnancy and still required to change the Tissue Tek chemicals, because "it wasn't fair". I lost my baby at 5 ? months. And was devastated, but still had to be back to work the next day, as not to put anyone out as to pick up my load. Plus there is more, but won't go into it. Needless to say, my manager NEVER stepped in to help me. Some managers need to be more proactive with their pregnant employees, regardless if is discriminating, use common sense. >>> "Stritmatter, Andrea" 2/18/2008 12:46 PM >>> >>> As a new mother of a 7 month old son and a manager of a histology lab, you have to work with the pregnant individual on this. It is discrimination (at least in Washington state) if forbid them to work in the lab. I chose not to take x-rays or handle DAB during my pregnancy. Otherwise I worked normally in the lab. I ended up with pregnancy complications and couldn't stand all day so I did a lot of paperwork catch up too. Ultimately, it's up to the manager and the employee to work together to find duties you all agree on. Your HR department should be able to assist in this too. Andrea Stritmatter, HTL(ASCP) MDSPS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, February 18, 2008 12:30 PM To: Douglas D Deltour; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Pregnancy Importance: Low Douglas: All chemicals used in the lab have TWA values LOWER for pregnant women and infants, and that is the reason why pregnant women should not work in the lab environment, regardles it may have TWA levels for adults within the limits. Ren? J. Douglas D Deltour wrote: No not me.. but. What are the regulations/guidelines for working in a histology lab while one is pregnant? Is this an individual choice or a doctor choice? Are there any liabilities if something happens? Thanks. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Tue Feb 19 19:39:20 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Feb 19 19:39:22 2008 Subject: [Histonet] Disposal of paraffin blocks References: <002601c87314$23f24800$1d2a14ac@wchsys.org> Message-ID: <007801c87361$6a649de0$0302a8c0@yourxhtr8hvc4p> We send ours to a medical waste company to be burned. JTT ----- Original Message ----- From: "Joyce Cline" To: Sent: Tuesday, February 19, 2008 10:26 AM Subject: [Histonet] Disposal of paraffin blocks > How are clinical labs disposing of their paraffin blocks? Regular trash, > incinerated trash or some medical waste company? We have several years > to get rid of and our boiler room won't let me send them down to be > incinerated. > > > ***** CONFIDENTIALITY NOTICE ***** > This message contains confidential information and is intended only for > the individual named. If you are not the named addressee you should not > disseminate, distribute or copy this e-mail. Please notify the sender > immediately by e-mail if you have received this e-mail by mistake and > delete this e-mail from your system. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Tue Feb 19 23:01:51 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Feb 19 23:01:57 2008 Subject: [Histonet] PFA degradation In-Reply-To: References: Message-ID: > > My question is...how do others commonly deal with PFA?? Do you make it > fresh each time you use it?? If so, what is the favorite protocol?? > And, just out of curiosity, has anyone ever done an analysis of stored > PFA to see how fast it breaks down...or how long it can safely be stored > and used?? We used to make 4% para in PBS, then freeze aliquots until we needed it. It always did the job well for embryonic chick tissue. Now we only use 4% para 24 hours after it was made according to Tom Jessell's protocol. (We switched to his because we received antibodies from him and wanted to make sure we followed his protocol completely.) I would love to know if someone has analyzed the degradation of para. My guess has always been that if it's not precipitated nor has something growing it (that would signal contamination that boggles my mind), it's good. I've also found that people in the lab like to blame para for the poor quality of their sections (quality of tissue and of staining). That isn't the problem; someone just didn't fix long enough or can't section well, etc. our old protocol: Boil 400 mL water with stir bar. Add 20 g paraformaldehyde, stir for around 5 min. Clear with 1N NaOH (I always use 12 drops which makes the pH perfect) pH to 7.2-7.5. Store at 4C or freeze into aliquots. Jessell protocol: http://sklad.cumc.columbia.edu/jessell/resources/protocols.php Emily -- fortune smiles on the brave and spits on the coward --aguirre, wrath of god From talulahgosh <@t> gmail.com Tue Feb 19 23:41:44 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Tue Feb 19 23:41:50 2008 Subject: [Histonet] quenching of Fluorescent signal In-Reply-To: <1198258712.219031203465236549.JavaMail.osg@osgjas03.cns.ufl.edu> References: <1198258712.219031203465236549.JavaMail.osg@osgjas03.cns.ufl.edu> Message-ID: Our Cy2/Cy3/Cy5 fluorescence lasts a few months at least--we mount with Fluoromount G from Biomedia. Vector usually has high quality products so I'd assume the problem isn't from their mounting media. Did someone bleach the signal by looking at it too long under a microscope? Maybe using Cy2 or Cy3 (or whatever 488 is) as a secondary would help also, since our labeling is always robust. You could always call Vector and ask if someone else has had the same problem. Or look on their website, they have protocols and troubleshooting. Emily -- fortune smiles on the brave and spits on the coward --aguirre, wrath of god From lpwenk <@t> sbcglobal.net Wed Feb 20 05:44:40 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed Feb 20 05:44:54 2008 Subject: [Histonet] Specimen Rejection - QA In-Reply-To: Message-ID: <000501c873b5$faa43740$0202a8c0@HPPav2> Thought this might be a start. Identification errors involving clinical laboratories: A College of American Pathologists Q-Probes Study of Patients and Specimen Identification Errors at 120 Institutions College of American Pathologists; Valenstein PN, Raab SS, Walsh MK Arch Pathol Lab Med. 2006 Aug;130(8):1106-13. Breaks it down into identification errors: - Primary specimen label error - Initial registration/order entry error - Other clerical error - Other reason for error - Aliquot/block/slide label error - Result entry error So as Rene said, you need to narrow your project down (just accessioning errors. Or just specimen container label errors. Or just slide label errors.). Than write all the ways there could be errors, as well as ways to identify WHO is doing the error. Such as for accessioning errors - what information was wrong - patient's name? spelling error? Wrong name? Hospital ID # - how was that wrong? Lab number - how was that wrong? Type of tissue - how was that wrong? Etc. And which person accessioned in the information? Peggy A. Wenk,HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, February 19, 2008 3:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Specimen Rejection - QA Hello Histonetters, I was wandering if anyone had any info or some kind of guidelines to help me get started on a QA study especially for specimen rejection. Thanks in advance, Amy Amy Self Georgetown Hospital Systems NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Erin.Martin <@t> ucsf.edu Wed Feb 20 07:33:56 2008 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Wed Feb 20 07:34:08 2008 Subject: [Histonet] Pregnancy Message-ID: When I was pregnant last year, I talked to my nurse midwife about the chemicals and she referred me to a California pregnancy safety hotline. I was doing gross and histo at the time. They went over all of the chemicals with me and basically agreed with everything that Laurie said - reduce exposure as much as possible and work in well ventilated areas. None of what I was working with is teratogenic (birth defect causing) but I was advised to be careful anyway. Compromising my own health wouldn't be good for the baby, obviously. Embedding and cutting are good options if you want to be extra careful. I'm sure that no-one would want to ask a nervous mom-to-be to do anything that might be unhealthy for her or her baby. It's not about legal accomodation, it's just the right thing to do. Your HR office probably has some sort of guidelines to help you if you are not sure. From sbreckenridge <@t> caperegional.com Wed Feb 20 08:46:56 2008 From: sbreckenridge <@t> caperegional.com (Breckenridge, Sue) Date: Wed Feb 20 08:47:04 2008 Subject: [Histonet] What is your experience with Histo-Clear II Message-ID: <4D95C24EC0E4C84787A6919F1165B25E015B20B5@btmhems01.BTHOSP.INT> I was hoping that any users (past or present) of the clearing agent Histo-Clear II would be willing to give me feedback on how they liked it. (For example, did it process tissues on par with other xylene substitutes; Is there no adverse effect on Immunohistochemistry staining; Can it be used for glass coverslipping & if so do the slides dry in a reasonable amount of time). Thank-you for taking the time to respond! Sue Breckenridge, Histology Supervisor Cape Regional Medical Center Cape May Court House, NJ From tanisha.mcknight <@t> covance.com Wed Feb 20 09:08:54 2008 From: tanisha.mcknight <@t> covance.com (McKnight, Tanisha) Date: Wed Feb 20 09:09:06 2008 Subject: [Histonet] Formalin and Xylene Free Histology - Any Info about Glyo Fixx Message-ID: <816E3C72F855F14985FC31D7C963AE6F04AEDF74@indexch03.ent.covance.com> Hello Again: I am looking into Microwave processing and using Glyo Fixx as the fixative. I am starting a histology lab, pretty much from scratch, but I have very little experience in using Formalin and Xylene alternatives. The question I have concerning the Glyo Fixx is whether or not I will need to use the same safety precautions we use when grossing/handling specimens fixed in formalin. Does it still need to be grossed under a hood? Are the ventilation requirements different. If anyone here is using this, can you tell me how you handle this. Tanisha N. McKnight, HT (ASCP) ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From doug <@t> ppspath.com Wed Feb 20 09:30:35 2008 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Wed Feb 20 09:31:28 2008 Subject: [Histonet] What is your experience with Histo-Clear II In-Reply-To: <4D95C24EC0E4C84787A6919F1165B25E015B20B5@btmhems01.BTHOSP.INT> Message-ID: <1008597632-78010902@pathology.swmed.edu> Sue, I have been trying the xylene substitutes also. I have tried about ten different brands and types of clearing reagent. First off, I can tell you that none of them are superior to xylene. After that realization I can tell you that the clearing agent that I chose was Citra-Clear from StatLab. It seems to clear better and faster than the other alternative clearing reagents (even Histo-Clear). If you are not fond of the citrus odor then the runner-up is Formula 83 from CBG Biotech. After months of testing, these were the top two alternative clearing reagents IMO. They are two different types clearing reagents though. Citra-Clear is citrus based (D-Limonene) and Formula 83 is a naphthenic hydrocarbon blend. Naphtha is refined petroleum fractions. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breckenridge, Sue Sent: Wednesday, February 20, 2008 9:47 AM To: histonet@pathology.swmed.edu Subject: [Histonet] What is your experience with Histo-Clear II I was hoping that any users (past or present) of the clearing agent Histo-Clear II would be willing to give me feedback on how they liked it. (For example, did it process tissues on par with other xylene substitutes; Is there no adverse effect on Immunohistochemistry staining; Can it be used for glass coverslipping & if so do the slides dry in a reasonable amount of time). Thank-you for taking the time to respond! Sue Breckenridge, Histology Supervisor Cape Regional Medical Center Cape May Court House, NJ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Wed Feb 20 09:37:57 2008 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Wed Feb 20 09:38:50 2008 Subject: SPAM-LOW: [Histonet] Formalin and Xylene Free Histology - Any Info about Glyo Fixx In-Reply-To: <816E3C72F855F14985FC31D7C963AE6F04AEDF74@indexch03.ent.covance.com> Message-ID: Tanisha, My question is why would you want to use a Formalin alternative? I hope you are talking about a research lab and are not concerned with validation of an alternative fixative. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McKnight, Tanisha Sent: Wednesday, February 20, 2008 10:09 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Formalin and Xylene Free Histology - Any Info about Glyo Fixx Hello Again: I am looking into Microwave processing and using Glyo Fixx as the fixative. I am starting a histology lab, pretty much from scratch, but I have very little experience in using Formalin and Xylene alternatives. The question I have concerning the Glyo Fixx is whether or not I will need to use the same safety precautions we use when grossing/handling specimens fixed in formalin. Does it still need to be grossed under a hood? Are the ventilation requirements different. If anyone here is using this, can you tell me how you handle this. Tanisha N. McKnight, HT (ASCP) ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From rjbuesa <@t> yahoo.com Wed Feb 20 09:41:44 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 20 09:41:48 2008 Subject: [Histonet] What is your experience with Histo-Clear II In-Reply-To: <4D95C24EC0E4C84787A6919F1165B25E015B20B5@btmhems01.BTHOSP.INT> Message-ID: <841403.32979.qm@web61219.mail.yahoo.com> Several postings on Histonet about Histoclear can be summarized as follows: 1- hardens brain, liver and spleen 2- not very good for coverslipping 3- poor dewaxing agent 4- not compatible with xylene/toluene based mounting media 5- reported as causing skin problems and headaches 6- fades hematoxylin. For more information consult Histonet archives under "Histoclear" Ren? J. "Breckenridge, Sue" wrote: --------------------------------- Never miss a thing. Make Yahoo your homepage. From NSEARCY <@t> swmail.sw.org Wed Feb 20 11:13:43 2008 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Wed Feb 20 11:13:53 2008 Subject: [Histonet] Dako Info Message-ID: Posting for Lead Immunohistochemistry Technologist: Anyone using Dako Envision & Detection system heard about it being discontinued? What substitutions are you considering? From ploykasek <@t> phenopath.com Wed Feb 20 11:27:48 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Wed Feb 20 11:28:03 2008 Subject: [Histonet] Dako Info In-Reply-To: Message-ID: I think I recall hearing that the Envision, not Envision+, was being discontinued, but I am not 100% positive. Patti Loykasek > Posting for Lead Immunohistochemistry Technologist: > > Anyone using Dako Envision & Detection system heard about it being > discontinued? What substitutions are you considering? > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From ploykasek <@t> phenopath.com Wed Feb 20 11:38:35 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Wed Feb 20 11:38:43 2008 Subject: [Histonet] IHC Detection systems Message-ID: Hi all. We are currently doing some preliminary evaluation of IHC small polymer detection systems. We currently use Envision+ detection. I was hoping others who have experience with the newer detections would be willing to share some insights - both positive and negative. I would be willing to provide feedback as we evaluate. Thank you. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From settembr <@t> umdnj.edu Wed Feb 20 11:40:34 2008 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Wed Feb 20 11:41:45 2008 Subject: [Histonet] Dako Info Message-ID: Patti is correct. I just spoke to Dako Tech who said that the Envision + will not be. They already discont. their "standard Envision" Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Patti Loykasek 02/20/08 12:27 PM >>> I think I recall hearing that the Envision, not Envision+, was being discontinued, but I am not 100% positive. Patti Loykasek > Posting for Lead Immunohistochemistry Technologist: > > Anyone using Dako Envision & Detection system heard about it being > discontinued? What substitutions are you considering? > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From daliaelrouby <@t> hotmail.com Wed Feb 20 11:47:44 2008 From: daliaelrouby <@t> hotmail.com (daliaelrouby@hotmail.com) Date: Wed Feb 20 11:47:53 2008 Subject: [Histonet] Join dalia el-rouby on Yahoo! Messenger! Message-ID: <1008589448-78503137@pathology.swmed.edu> dalia el-rouby wants to talk with you using Yahoo! Messenger: Accept the invitation by clicking this link: http://invite.msg.yahoo.com/invite?op=accept&intl=us&sig=.TpgZX71RpMOVEbAQPzxDfxbG1jwwymGHyezPV.qOYthzoAUSN8pqyDXA1wH3F7J6xkXI3v62dzSX3mf2zh7IcKmCy5NHNDKTGHVOsVRN62VgcEmnF8NaEsFssOA8BhI4qD3SLPCFjwikz_sAgwwkkJWlw-- With Yahoo! Messenger, you get: Free worldwide PC-to-PC calls.* All you need are speakers and a microphone (or a headset). If no one's there, leave a voicemail! IM Windows Live™ Messenger friends too. Add your Windows Live friends to your Yahoo! contact list. See when they're online and IM them anytime. Stealth settings keep you in control. Now you can get in touch on your time, by controlling who sees when you're online. So what are you waiting for? It's free. Get Yahoo! Messenger and start connecting how you want, when you want. * Emergency 911 calling services not available on Yahoo! Messenger. Please inform others who use your Yahoo! Messenger they must dial 911 through traditional phone lines or cell carriers. By using Yahoo! Messenger you agree to not use PC-to-PC calling in countries where prohibited. The above features apply to the Windows version of Yahoo! Messenger. From JWEEMS <@t> sjha.org Wed Feb 20 11:49:33 2008 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Feb 20 11:49:39 2008 Subject: [Histonet] Dako Info In-Reply-To: References: Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD320518E70E@sjhaexc02.sjha.org> Correct. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Wednesday, February 20, 2008 12:28 PM To: Nita Searcy; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Dako Info I think I recall hearing that the Envision, not Envision+, was being discontinued, but I am not 100% positive. Patti Loykasek > Posting for Lead Immunohistochemistry Technologist: > > Anyone using Dako Envision & Detection system heard about it being > discontinued? What substitutions are you considering? > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From lcates <@t> synecor.com Wed Feb 20 11:57:25 2008 From: lcates <@t> synecor.com (Lynne Cates) Date: Wed Feb 20 11:57:42 2008 Subject: [Histonet] Adhesive tape & MMA Message-ID: Does anyone have any experience using scotch tape staining, adherence to slide on MMA sections? Thanks in advance. Lynne Cates, HT (ASCP) Supervisor Histopathology Services SyneCor, LLC 3908 Patriot Drive Suite 170 Durham, NC 27703 Tel (919) 541-9977 X 119 Fax (919) 541-9975 From laurie.colbert <@t> huntingtonhospital.com Wed Feb 20 12:15:40 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed Feb 20 12:15:47 2008 Subject: [Histonet] HELP!! With tissue processing Message-ID: <57BE698966D5C54EAE8612E8941D7683027B0D93@EXCHANGE3.huntingtonhospital.com> I'm hoping someone can give me some advice on fixing our biopsy specimens that were "fried" last night during processing. Someone put a 100% alcohol where the last xylene (before the paraffin) should have been. The problem was discovered during embedding. At this point, someone switched out the cleaning alcohol bottle with xylene, and put the tissue through two changes of xylene on the clean cycle. I think this is what fried the tissues. The tissue is very dry and unreadable, and needless to say, the pathologists are livid! I would appreciate any help you can give. Laurie Colbert From PMonfils <@t> Lifespan.org Wed Feb 20 12:29:51 2008 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Feb 20 12:29:57 2008 Subject: [Histonet] HELP!! With tissue processing In-Reply-To: <57BE698966D5C54EAE8612E8941D7683027B0D93@EXCHANGE3.huntingtonhospital.com> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273D24@LSRIEXCH1.lsmaster.lifespan.org> If the problem was absolute alcohol between the xylenes and the paraffin, the tissue should be rerun through fresh xylenes to remove both the poorly infiltrated paraffin and any remaining alcohol. Then through fresh xylenes to reinfiltrate the tissue properly. I can't guarantee there won't be any negative effects, but this is about all you can do at this point, and hopefully the tissues will section a lot better after this treatment. If you have vacuum available on your processor, use it on all stations during this rerun. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Laurie Colbert > Sent: Wednesday, February 20, 2008 1:15 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] HELP!! With tissue processing > > I'm hoping someone can give me some advice on fixing our biopsy > specimens that were "fried" last night during processing. > > > > Someone put a 100% alcohol where the last xylene (before the paraffin) > should have been. The problem was discovered during embedding. At this > point, someone switched out the cleaning alcohol bottle with xylene, and > put the tissue through two changes of xylene on the clean cycle. I > think this is what fried the tissues. The tissue is very dry and > unreadable, and needless to say, the pathologists are livid! I would > appreciate any help you can give. > > > > Laurie Colbert > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From trathborne <@t> somerset-healthcare.com Wed Feb 20 13:16:14 2008 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Wed Feb 20 13:34:49 2008 Subject: [Histonet] Scabies Message-ID: Hi all! I have a question about the proper way to process/prepare skin scrapings for scabies. We have had a few cases in the last year, and want to be sure that they are being submitted properly. Also, does anyone know where we might purchase a positive slide/picture to identify the eggs. With something that occurs so infrequently, the pathologist's would like to have a reference so that they know what they are looking for. Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. From rcharles <@t> state.pa.us Wed Feb 20 14:26:05 2008 From: rcharles <@t> state.pa.us (Charles, Roger) Date: Wed Feb 20 14:26:52 2008 Subject: [Histonet] Scabies Message-ID: <12E4E17FEF6EBE4BAE95BEB3CDCB57000C79D155@enhbgpri04.backup> There is good information and illustrations on human scabies on MedlinePlus. Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, February 20, 2008 2:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Scabies Hi all! I have a question about the proper way to process/prepare skin scrapings for scabies. We have had a few cases in the last year, and want to be sure that they are being submitted properly. Also, does anyone know where we might purchase a positive slide/picture to identify the eggs. With something that occurs so infrequently, the pathologist's would like to have a reference so that they know what they are looking for. Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. From oshel1pe <@t> cmich.edu Wed Feb 20 14:38:08 2008 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Wed Feb 20 14:38:17 2008 Subject: [Histonet] text recommendation Message-ID: Listers, We're thinking of changing our Light Microscopy course text, and I thought I'd check with the histo gang. What we're looking for is a text that covers both the construction, optics (with some theory) and use of the light microscope, with DIC, Hoffman, fluorescence, etc. *and* microtechnique, preferably animal. Fixation, dehydration, embedding, sectioning, etc. I've got excellent examples of books that cover either the microscope *or* microtechnique, but not both. And, those are expensive. We're also hoping find something reasonably priced, not $80 to over $100, as most texts seem to be these days. (If necessary, we'll go with 2 books, one on the microscope and optics, and one on microtechnique, but again, price counts. 2 book, each one costing $60 or more is right out.) Any ideas? Thanks. Phil -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576 From mcauliff <@t> umdnj.edu Wed Feb 20 15:37:39 2008 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Feb 20 15:37:42 2008 Subject: [Histonet] text recommendation In-Reply-To: References: Message-ID: <47BC9DA3.7040103@umdnj.edu> Hi Phil: I have the prefect text for you. "The Complete Microscopist" by Oshel et al. Seriously, I doubt if the book you want exists. More than a few people have started out with a syllabus and ultimately turned it into a textbook. Geoff Philip Oshel wrote: > Listers, > > We're thinking of changing our Light Microscopy course text, and I > thought I'd check with the histo gang. > What we're looking for is a text that covers both the construction, > optics (with some theory) and use of the light microscope, with DIC, > Hoffman, fluorescence, etc. *and* microtechnique, preferably animal. > Fixation, dehydration, embedding, sectioning, etc. > I've got excellent examples of books that cover either the microscope > *or* microtechnique, but not both. > And, those are expensive. > We're also hoping find something reasonably priced, not $80 to over > $100, as most texts seem to be these days. > (If necessary, we'll go with 2 books, one on the microscope and > optics, and one on microtechnique, but again, price counts. 2 book, > each one costing $60 or more is right out.) > Any ideas? > Thanks. > > Phil -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From ploykasek <@t> phenopath.com Wed Feb 20 17:03:00 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Wed Feb 20 17:03:11 2008 Subject: [Histonet] Microtomes Message-ID: Thanks to all who have replied to my earlier questions about small polymer IHC detections. Now I have another question. I am working on next year's budget and looking at a semi-automatic microtome. I would appreciate any feedback - good & bad- on these type of microtomes. As always, thanks for the help. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From sprice2003 <@t> gmail.com Wed Feb 20 17:49:17 2008 From: sprice2003 <@t> gmail.com (Sally Price) Date: Wed Feb 20 17:49:26 2008 Subject: [Histonet] quenching of Fluorescent signal Message-ID: Ann: I've had exceptionally good results with Biocare's anti-mouse and anti-rabbit Dylight-488 followed by DAP, and the signal lasts for weeks. Biocare also offers a product called FluoroCare 'anti-fade' medium Good Luck! -- Sally ------------------------------ Message: 17 Date: Tue, 19 Feb 2008 18:53:56 -0500 (EST) From: "FU,DONGTAO" Subject: [Histonet] quenching of Fluorescent signal To: Histonet@lists.utsouthwestern.edu Hi, all I am doing fluorescent staining on fresh frozen human spleen using CD markers, like CD4, CD8, etc these days. The secondary antibody I used is AF 488, the mount media was DAPI(hard set) from vector. I can get very nice staining, however, I find these signals only can be kept for a couple of days, then start to quench deeply, although I put my stained slides in 4C. Does anyone have experience on how to keep fluorecent signal last longer? And usually how long do you still get your IF signal after mounting? Thank you, Ann Dongtao Fu MD, Ph.D Lab Manager Dept. of Pathology Lab phone: 352-273-7752 Lab FAX: 352-273-7755 Lab address: D11-50 PO Box: 100275 1600 SW Archer Road University of Flodrida Gainesville, FL 32610 From sprice2003 <@t> gmail.com Wed Feb 20 17:55:11 2008 From: sprice2003 <@t> gmail.com (Sally Price) Date: Wed Feb 20 17:55:16 2008 Subject: [Histonet] Dako Info Message-ID: Nita: I've heard that envision-plus and LSAB2 will be discontinued, and that Dako wants to encourage folks to use a new 2-part polymer system called FLEX -- which is supposed to cost a ton more than EP/EP-DualLink. Anybody have confirmation of this? -- Sally ------------------------------ Message: 8 Date: Wed, 20 Feb 2008 11:13:43 -0600 From: "Nita Searcy" Subject: [Histonet] Dako Info To: histonet@lists.utsouthwestern.edu Posting for Lead Immunohistochemistry Technologist: Anyone using Dako Envision & Detection system heard about it being discontinued? What substitutions are you considering? From Melissa.Gonzalez <@t> cellgenesys.com Wed Feb 20 19:15:18 2008 From: Melissa.Gonzalez <@t> cellgenesys.com (Melissa Gonzalez) Date: Wed Feb 20 19:15:25 2008 Subject: [Histonet] Molecular Probes Zenon labeling kits Message-ID: <2884B897182A1D438C7BA24B9A8F94A22674BA@hqsvr01mail.cgi.com> Has anyone used the labeling kits (Zenon) from Invitrogen to directly label their abs? How well do they work? They show fabulous examples online of cell staining, but I was wondering how practical they were for tissue staining and epifluorescence detection. Thanks, Melissa Melissa A. Gonz?lez Edick R&D, Cell Genesys Inc. 500 Forbes Blvd South San Francisco, CA 94080 p(650) 266-3168 f (650) 266-3080 From amosbrooks <@t> gmail.com Wed Feb 20 19:22:48 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Feb 20 19:22:52 2008 Subject: [Histonet] Formalin and Xylene Free Histology - Any Info Message-ID: <582736990802201722n1fde9a33hc7ad102ff3d34f36@mail.gmail.com> My standard formalin alternative rant was sent privately ... if anyone is interested see the archives. No sense reposting my redundant disagreement with this concept! Amos Brooks Message: 4 Date: Wed, 20 Feb 2008 10:08:54 -0500 From: "McKnight, Tanisha" Subject: [Histonet] Formalin and Xylene Free Histology - Any Info about Glyo Fixx To: histonet@lists.utsouthwestern.edu Message-ID: <816E3C72F855F14985FC31D7C963AE6F04AEDF74@indexch03.ent.covance.com> Content-Type: text/plain; charset="us-ascii" Hello Again: I am looking into Microwave processing and using Glyo Fixx as the fixative. I am starting a histology lab, pretty much from scratch, but I have very little experience in using Formalin and Xylene alternatives. The question I have concerning the Glyo Fixx is whether or not I will need to use the same safety precautions we use when grossing/handling specimens fixed in formalin. Does it still need to be grossed under a hood? Are the ventilation requirements different. If anyone here is using this, can you tell me how you handle this. Tanisha N. McKnight, HT (ASCP) From mohs76009 <@t> yahoo.com Wed Feb 20 20:25:15 2008 From: mohs76009 <@t> yahoo.com (Matt Bancroft) Date: Wed Feb 20 20:25:20 2008 Subject: [Histonet] Scabies In-Reply-To: Message-ID: <815001.9685.qm@web63405.mail.re1.yahoo.com> I have tried to e-mail you a photo of scabies but it comes back, do you have a different e-mail address "Rathborne, Toni" wrote: Hi all! I have a question about the proper way to process/prepare skin scrapings for scabies. We have had a few cases in the last year, and want to be sure that they are being submitted properly. Also, does anyone know where we might purchase a positive slide/picture to identify the eggs. With something that occurs so infrequently, the pathologist's would like to have a reference so that they know what they are looking for. Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From R.vandenBerg <@t> sakura.eu Wed Feb 20 20:54:03 2008 From: R.vandenBerg <@t> sakura.eu (Ralf van den Berg) Date: Wed Feb 20 20:54:18 2008 Subject: [Histonet] RE: Histonet Digest, Vol 51, Issue 31 Message-ID: -----Oorspronkelijk bericht----- Van: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Namens histonet-request@lists.utsouthwestern.edu Verzonden: woensdag 20 februari 2008 19:35 Aan: histonet@lists.utsouthwestern.edu Onderwerp: Histonet Digest, Vol 51, Issue 31 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Specimen Rejection - QA (Lee & Peggy Wenk) 2. Pregnancy (Martin, Erin) 3. What is your experience with Histo-Clear II (Breckenridge, Sue) 4. Formalin and Xylene Free Histology - Any Info about Glyo Fixx (McKnight, Tanisha) 5. RE: What is your experience with Histo-Clear II (Douglas D Deltour) 6. RE: SPAM-LOW: [Histonet] Formalin and Xylene Free Histology - Any Info about Glyo Fixx (Douglas D Deltour) 7. Re: What is your experience with Histo-Clear II (Rene J Buesa) 8. Dako Info (Nita Searcy) 9. Re: Dako Info (Patti Loykasek) 10. IHC Detection systems (Patti Loykasek) 11. Re: Dako Info (Dana Settembre) 12. Join dalia el-rouby on Yahoo! Messenger! (daliaelrouby@hotmail.com) 13. RE: Dako Info (Weems, Joyce) 14. Adhesive tape & MMA (Lynne Cates) ---------------------------------------------------------------------- Message: 1 Date: Wed, 20 Feb 2008 06:44:40 -0500 From: "Lee & Peggy Wenk" Subject: RE: [Histonet] Specimen Rejection - QA To: "'Amy Self'" , Message-ID: <000501c873b5$faa43740$0202a8c0@HPPav2> Content-Type: text/plain; charset="us-ascii" Thought this might be a start. Identification errors involving clinical laboratories: A College of American Pathologists Q-Probes Study of Patients and Specimen Identification Errors at 120 Institutions College of American Pathologists; Valenstein PN, Raab SS, Walsh MK Arch Pathol Lab Med. 2006 Aug;130(8):1106-13. Breaks it down into identification errors: - Primary specimen label error - Initial registration/order entry error - Other clerical error - Other reason for error - Aliquot/block/slide label error - Result entry error So as Rene said, you need to narrow your project down (just accessioning errors. Or just specimen container label errors. Or just slide label errors.). Than write all the ways there could be errors, as well as ways to identify WHO is doing the error. Such as for accessioning errors - what information was wrong - patient's name? spelling error? Wrong name? Hospital ID # - how was that wrong? Lab number - how was that wrong? Type of tissue - how was that wrong? Etc. And which person accessioned in the information? Peggy A. Wenk,HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, February 19, 2008 3:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Specimen Rejection - QA Hello Histonetters, I was wandering if anyone had any info or some kind of guidelines to help me get started on a QA study especially for specimen rejection. Thanks in advance, Amy Amy Self Georgetown Hospital Systems NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 20 Feb 2008 05:33:56 -0800 From: "Martin, Erin" Subject: [Histonet] Pregnancy To: "histonet" Message-ID: Content-Type: text/plain; charset=iso-8859-1 When I was pregnant last year, I talked to my nurse midwife about the chemicals and she referred me to a California pregnancy safety hotline. I was doing gross and histo at the time. They went over all of the chemicals with me and basically agreed with everything that Laurie said - reduce exposure as much as possible and work in well ventilated areas. None of what I was working with is teratogenic (birth defect causing) but I was advised to be careful anyway. Compromising my own health wouldn't be good for the baby, obviously. Embedding and cutting are good options if you want to be extra careful. I'm sure that no-one would want to ask a nervous mom-to-be to do anything that might be unhealthy for her or her baby. It's not about legal accomodation, it's just the right thing to do. Your HR office probably has some sort of guidelines to help you if you are not sure. ------------------------------ Message: 3 Date: Wed, 20 Feb 2008 09:46:56 -0500 From: "Breckenridge, Sue" Subject: [Histonet] What is your experience with Histo-Clear II To: Message-ID: <4D95C24EC0E4C84787A6919F1165B25E015B20B5@btmhems01.BTHOSP.INT> Content-Type: text/plain; charset="us-ascii" I was hoping that any users (past or present) of the clearing agent Histo-Clear II would be willing to give me feedback on how they liked it. (For example, did it process tissues on par with other xylene substitutes; Is there no adverse effect on Immunohistochemistry staining; Can it be used for glass coverslipping & if so do the slides dry in a reasonable amount of time). Thank-you for taking the time to respond! Sue Breckenridge, Histology Supervisor Cape Regional Medical Center Cape May Court House, NJ ------------------------------ Message: 4 Date: Wed, 20 Feb 2008 10:08:54 -0500 From: "McKnight, Tanisha" Subject: [Histonet] Formalin and Xylene Free Histology - Any Info about Glyo Fixx To: histonet@lists.utsouthwestern.edu Message-ID: <816E3C72F855F14985FC31D7C963AE6F04AEDF74@indexch03.ent.covance.com> Content-Type: text/plain; charset="us-ascii" Hello Again: I am looking into Microwave processing and using Glyo Fixx as the fixative. I am starting a histology lab, pretty much from scratch, but I have very little experience in using Formalin and Xylene alternatives. The question I have concerning the Glyo Fixx is whether or not I will need to use the same safety precautions we use when grossing/handling specimens fixed in formalin. Does it still need to be grossed under a hood? Are the ventilation requirements different. If anyone here is using this, can you tell me how you handle this. Tanisha N. McKnight, HT (ASCP) ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. ------------------------------ Message: 5 Date: Wed, 20 Feb 2008 10:30:35 -0500 From: "Douglas D Deltour" Subject: RE: [Histonet] What is your experience with Histo-Clear II To: "'Breckenridge, Sue'" , Message-ID: <1008597632-78010902@pathology.swmed.edu> Content-Type: text/plain; charset="us-ascii" Sue, I have been trying the xylene substitutes also. I have tried about ten different brands and types of clearing reagent. First off, I can tell you that none of them are superior to xylene. After that realization I can tell you that the clearing agent that I chose was Citra-Clear from StatLab. It seems to clear better and faster than the other alternative clearing reagents (even Histo-Clear). If you are not fond of the citrus odor then the runner-up is Formula 83 from CBG Biotech. After months of testing, these were the top two alternative clearing reagents IMO. They are two different types clearing reagents though. Citra-Clear is citrus based (D-Limonene) and Formula 83 is a naphthenic hydrocarbon blend. Naphtha is refined petroleum fractions. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breckenridge, Sue Sent: Wednesday, February 20, 2008 9:47 AM To: histonet@pathology.swmed.edu Subject: [Histonet] What is your experience with Histo-Clear II I was hoping that any users (past or present) of the clearing agent Histo-Clear II would be willing to give me feedback on how they liked it. (For example, did it process tissues on par with other xylene substitutes; Is there no adverse effect on Immunohistochemistry staining; Can it be used for glass coverslipping & if so do the slides dry in a reasonable amount of time). Thank-you for taking the time to respond! Sue Breckenridge, Histology Supervisor Cape Regional Medical Center Cape May Court House, NJ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Wed, 20 Feb 2008 10:37:57 -0500 From: "Douglas D Deltour" Subject: RE: SPAM-LOW: [Histonet] Formalin and Xylene Free Histology - Any Info about Glyo Fixx To: "'McKnight, Tanisha'" , Message-ID: Content-Type: text/plain; charset="us-ascii" Tanisha, My question is why would you want to use a Formalin alternative? I hope you are talking about a research lab and are not concerned with validation of an alternative fixative. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McKnight, Tanisha Sent: Wednesday, February 20, 2008 10:09 AM To: histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: [Histonet] Formalin and Xylene Free Histology - Any Info about Glyo Fixx Hello Again: I am looking into Microwave processing and using Glyo Fixx as the fixative. I am starting a histology lab, pretty much from scratch, but I have very little experience in using Formalin and Xylene alternatives. The question I have concerning the Glyo Fixx is whether or not I will need to use the same safety precautions we use when grossing/handling specimens fixed in formalin. Does it still need to be grossed under a hood? Are the ventilation requirements different. If anyone here is using this, can you tell me how you handle this. Tanisha N. McKnight, HT (ASCP) ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. ------------------------------ Message: 7 Date: Wed, 20 Feb 2008 07:41:44 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] What is your experience with Histo-Clear II To: "Breckenridge, Sue" , histonet@pathology.swmed.edu Message-ID: <841403.32979.qm@web61219.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Several postings on Histonet about Histoclear can be summarized as follows: 1- hardens brain, liver and spleen 2- not very good for coverslipping 3- poor dewaxing agent 4- not compatible with xylene/toluene based mounting media 5- reported as causing skin problems and headaches 6- fades hematoxylin. For more information consult Histonet archives under "Histoclear" Ren? J. "Breckenridge, Sue" wrote: --------------------------------- Never miss a thing. Make Yahoo your homepage. ------------------------------ Message: 8 Date: Wed, 20 Feb 2008 11:13:43 -0600 From: "Nita Searcy" Subject: [Histonet] Dako Info To: Message-ID: Content-Type: text/plain; charset=US-ASCII Posting for Lead Immunohistochemistry Technologist: Anyone using Dako Envision & Detection system heard about it being discontinued? What substitutions are you considering? ------------------------------ Message: 9 Date: Wed, 20 Feb 2008 09:27:48 -0800 From: Patti Loykasek Subject: Re: [Histonet] Dako Info To: Nita Searcy , Message-ID: Content-Type: text/plain; charset="US-ASCII" I think I recall hearing that the Envision, not Envision+, was being discontinued, but I am not 100% positive. Patti Loykasek > Posting for Lead Immunohistochemistry Technologist: > > Anyone using Dako Envision & Detection system heard about it being > discontinued? What substitutions are you considering? > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ------------------------------ Message: 10 Date: Wed, 20 Feb 2008 09:38:35 -0800 From: Patti Loykasek Subject: [Histonet] IHC Detection systems To: histonet Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi all. We are currently doing some preliminary evaluation of IHC small polymer detection systems. We currently use Envision+ detection. I was hoping others who have experience with the newer detections would be willing to share some insights - both positive and negative. I would be willing to provide feedback as we evaluate. Thank you. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ------------------------------ Message: 11 Date: Wed, 20 Feb 2008 12:40:34 -0500 From: Dana Settembre Subject: Re: [Histonet] Dako Info To: histonet@lists.utsouthwestern.edu, Patti Loykasek , Nita Searcy Message-ID: Content-Type: text/plain; charset=US-ASCII Patti is correct. I just spoke to Dako Tech who said that the Envision + will not be. They already discont. their "standard Envision" Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Patti Loykasek 02/20/08 12:27 PM >>> I think I recall hearing that the Envision, not Envision+, was being discontinued, but I am not 100% positive. Patti Loykasek > Posting for Lead Immunohistochemistry Technologist: > > Anyone using Dako Envision & Detection system heard about it being > discontinued? What substitutions are you considering? > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: 20 Feb 2008 09:47:44 -0800 From: daliaelrouby@hotmail.com Subject: [Histonet] Join dalia el-rouby on Yahoo! Messenger! To: histonet@pathology.swmed.edu Message-ID: <1008589448-78503137@pathology.swmed.edu> Content-Type: text/plain; charset=en_US.ISO-8859-1 dalia el-rouby wants to talk with you using Yahoo! Messenger: Accept the invitation by clicking this link: http://invite.msg.yahoo.com/invite?op=accept&intl=us&sig=.TpgZX71RpMOVEbAQPzxDfxbG1jwwymGHyezPV.qOYthzoAUSN8pqyDXA1wH3F7J6xkXI3v62dzSX3mf2zh7IcKmCy5NHNDKTGHVOsVRN62VgcEmnF8NaEsFssOA8BhI4qD3SLPCFjwikz_sAgwwkkJWlw-- With Yahoo! Messenger, you get: Free worldwide PC-to-PC calls.* All you need are speakers and a microphone (or a headset). If no one's there, leave a voicemail! IM Windows Live™ Messenger friends too. Add your Windows Live friends to your Yahoo! contact list. See when they're online and IM them anytime. Stealth settings keep you in control. Now you can get in touch on your time, by controlling who sees when you're online. So what are you waiting for? It's free. Get Yahoo! Messenger and start connecting how you want, when you want. * Emergency 911 calling services not available on Yahoo! Messenger. Please inform others who use your Yahoo! Messenger they must dial 911 through traditional phone lines or cell carriers. By using Yahoo! Messenger you agree to not use PC-to-PC calling in countries where prohibited. The above features apply to the Windows version of Yahoo! Messenger. ------------------------------ Message: 13 Date: Wed, 20 Feb 2008 12:49:33 -0500 From: "Weems, Joyce" Subject: RE: [Histonet] Dako Info To: "Patti Loykasek" , "Nita Searcy" , Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD320518E70E@sjhaexc02.sjha.org> Content-Type: text/plain; charset="us-ascii" Correct. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Wednesday, February 20, 2008 12:28 PM To: Nita Searcy; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Dako Info I think I recall hearing that the Envision, not Envision+, was being discontinued, but I am not 100% positive. Patti Loykasek > Posting for Lead Immunohistochemistry Technologist: > > Anyone using Dako Envision & Detection system heard about it being > discontinued? What substitutions are you considering? > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. ------------------------------ Message: 14 Date: Wed, 20 Feb 2008 12:57:25 -0500 From: "Lynne Cates" Subject: [Histonet] Adhesive tape & MMA To: Message-ID: Content-Type: text/plain; charset="us-ascii" Does anyone have any experience using scotch tape staining, adherence to slide on MMA sections? Thanks in advance. Lynne Cates, HT (ASCP) Supervisor Histopathology Services SyneCor, LLC 3908 Patriot Drive Suite 170 Durham, NC 27703 Tel (919) 541-9977 X 119 Fax (919) 541-9975 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 51, Issue 31 **************************************** Confidentiality Note: This information and the attached file(s) are considered trade secret, confidential and/or property by Sakura Finetek Europe B.V. Any use, disclosure of reproduction of these documents by anyone other than the addressee is prohibited. If this E-mail has been received by anyone other than the addressee, please call +31-71-58.98.300 immediately to arrange for the document(s) to be returned. Sakura can never be legally bound by the acceptance of any supposed offer in an electronic message. Please reply or send your e-mail to: sakura@sakura.eu Sakura Finetek Europe B.V. P.O. Box 40, 2380 AA Hoge Rijndijk 48a 2382 AT Zoeterwoude The Netherlands Tel. +31 (0) 71 589 83 00 Fax +31 (0) 71 589 84 88 KvK/Chamber of Commerce Leiden 28065449 From tkngflght <@t> yahoo.com Wed Feb 20 17:50:54 2008 From: tkngflght <@t> yahoo.com (Cheryl) Date: Thu Feb 21 03:30:47 2008 Subject: [Histonet] Blair or other used equipment resources? In-Reply-To: <47BC9DA3.7040103@umdnj.edu> References: <47BC9DA3.7040103@umdnj.edu> Message-ID: <01a201c8741b$7371dc80$6601a8c0@FSROGER> Hi--could someone help with a phone number for used/refurbished equipment companies? There is a fellow (Mr. Royer?) who posts sometimes? Help? Thank you! Cheryl Full Staff Inc. From max_histo_00 <@t> yahoo.it Thu Feb 21 03:50:07 2008 From: max_histo_00 <@t> yahoo.it (Massimo) Date: Thu Feb 21 03:50:19 2008 Subject: [Histonet] Thanks ... Message-ID: <844878.62735.qm@web23310.mail.ird.yahoo.com> Thanks for all the replies to my questions! Massimo --------------------------------- --------------------------------- L'email della prossima generazione? Puoi averla con la nuova Yahoo! Mail From immrstambo <@t> hotmail.com Thu Feb 21 06:05:13 2008 From: immrstambo <@t> hotmail.com (Christine Tambasco) Date: Thu Feb 21 06:05:18 2008 Subject: [Histonet] Microtomes In-Reply-To: References: Message-ID: Patti- We have the Leica RM2155 and RM2255. They both work very well and you can either hand crank, use a foot pedal like a sewing machine or a countertop touch pad. We have gotten very used to the foot pedal and we love them! You can cut 3 microns with no problems. Most all tissue (except really hard bone) cuts like butter with beautiful, even ribbons. I havent tried any others though. They hooked me in right from the demo and we have had them about 7-8 years. Good luck! Christine Tambasco HT (ASCP) St. Mary's Hospital Amsteram, New York> Date: Wed, 20 Feb 2008 15:03:00 -0800> From: ploykasek@phenopath.com> To: histonet@pathology.swmed.edu> CC: > Subject: [Histonet] Microtomes> > Thanks to all who have replied to my earlier questions about small polymer> IHC detections. Now I have another question. I am working on next year's> budget and looking at a semi-automatic microtome. I would appreciate any> feedback - good & bad- on these type of microtomes. As always, thanks for> the help. > > > Patti Loykasek BS, HTL, QIHC> PhenoPath Laboratories> Seattle, WA> > > > > This e-mail message, including any attachments, is for the sole use of the > intended recipients and may contain privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not the intended > recipient, please contact the sender by e-mail and destroy all copies of the > original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. > at (206) 374-9000.> > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Helping your favorite cause is as easy as instant messaging.?You IM, we give. http://im.live.com/Messenger/IM/Home/?source=text_hotmail_join From doug <@t> ppspath.com Thu Feb 21 06:32:32 2008 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Thu Feb 21 06:33:27 2008 Subject: SPAM-LOW: [Histonet] Blair or other used equipment resources? In-Reply-To: <01a201c8741b$7371dc80$6601a8c0@FSROGER> Message-ID: <1008521918-82564838@pathology.swmed.edu> Southeast Pathology Instrument (843) 588-2559 Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Wednesday, February 20, 2008 6:51 PM To: Histonet@Pathology.swmed.edu Cc: Histonet@Pathology.swmed.edu Subject: SPAM-LOW: [Histonet] Blair or other used equipment resources? Hi--could someone help with a phone number for used/refurbished equipment companies? There is a fellow (Mr. Royer?) who posts sometimes? Help? Thank you! Cheryl Full Staff Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From micro <@t> superlink.net Thu Feb 21 06:33:22 2008 From: micro <@t> superlink.net (Markus F. Meyenhofer) Date: Thu Feb 21 06:33:49 2008 Subject: [Histonet] Blair or other used equipment resources? References: <47BC9DA3.7040103@umdnj.edu> <01a201c8741b$7371dc80$6601a8c0@FSROGER> Message-ID: <055e01c87485$f4732b80$6e893cd1@DJ4VDH31> Microscopy Laboratories P.O.Box 338 61 West Street Red Bank, NJ 07701 732 747 6228 fax 732 758 9142 Pre-owned EM-, Histology- and Lab Equipment. ----- Original Message ----- From: "Cheryl" To: Cc: Sent: Wednesday, February 20, 2008 6:50 PM Subject: [Histonet] Blair or other used equipment resources? > > Hi--could someone help with a phone number for used/refurbished equipment > companies? There is a fellow (Mr. Royer?) who posts sometimes? Help? > > Thank you! > > Cheryl > Full Staff Inc. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From micro <@t> superlink.net Thu Feb 21 06:33:22 2008 From: micro <@t> superlink.net (Markus F. Meyenhofer) Date: Thu Feb 21 06:33:52 2008 Subject: [Histonet] Blair or other used equipment resources? References: <47BC9DA3.7040103@umdnj.edu> <01a201c8741b$7371dc80$6601a8c0@FSROGER> Message-ID: <055e01c87485$f4732b80$6e893cd1@DJ4VDH31> Microscopy Laboratories P.O.Box 338 61 West Street Red Bank, NJ 07701 732 747 6228 fax 732 758 9142 Pre-owned EM-, Histology- and Lab Equipment. ----- Original Message ----- From: "Cheryl" To: Cc: Sent: Wednesday, February 20, 2008 6:50 PM Subject: [Histonet] Blair or other used equipment resources? > > Hi--could someone help with a phone number for used/refurbished equipment > companies? There is a fellow (Mr. Royer?) who posts sometimes? Help? > > Thank you! > > Cheryl > Full Staff Inc. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From nancy.troiano <@t> yale.edu Thu Feb 21 06:33:50 2008 From: nancy.troiano <@t> yale.edu (Nancy W. Troiano) Date: Thu Feb 21 06:34:05 2008 Subject: [Histonet] MMA and tape Message-ID: <5.2.1.1.2.20080221073052.020d8d78@email.med.yale.edu> I regularly use clear packing tape with MMA on my more difficult sections (e.g. mouse mandibles with teeth). Just cut to size of block face leaving tab of tape on bottom, rub tape gently on block face to adhere tape to block, moisten knife with 70% ethanol and pull tape very gently upward while microtome is cutting downward. Use chrome alum gel coated slides and put in 37 deg oven for two nights (or 60 deg oven for one nite if not doing enzyme stains). Soak slides in toluene to remove tape, deplastify and stain. Hope this helps. From tkngflght <@t> yahoo.com Thu Feb 21 07:52:45 2008 From: tkngflght <@t> yahoo.com (Cheryl) Date: Thu Feb 21 07:52:56 2008 Subject: [Histonet] Help with rodent tissue Message-ID: <276107.88664.qm@web50907.mail.re2.yahoo.com> Hi all~ I would appreciate some suggestions on this problem: I'm having trouble with rodent research tissue. It arrives already decaled, processed (yes, it is overprocessed). I'm willing to try anything at this point. We've tried a number of things and rather than listing the things we've tried (in the interest of gaining the benefit of all the histotnet's wealth of knowledge from you guys!!) ---I'm open to any and all suggestions!!! THANK YOU! Cheryl Full Staff Inc. Staffing the lab -- One GREAT tech at a time. 281.852.9457 office 281.883.7704 800.756.3309 fax From GauchV <@t> mail.amc.edu Thu Feb 21 08:10:58 2008 From: GauchV <@t> mail.amc.edu (Vicki Gauch) Date: Thu Feb 21 08:11:42 2008 Subject: [Histonet] HistoBath Message-ID: Hi everyone, Our isopentane bath (Histo Bath) that we use to freeze our frozen section blocks has finally stopped working and cannot be repaired. We tried to order a replacement unit only to find out that they no longer make it !!! The company also did not have another option. Does anyone know where we can get something like this? Any help would be greatly appreciated.. Thanks, Vicki Gauch AMCH Albany, NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. From froyer <@t> bitstream.net Thu Feb 21 08:25:45 2008 From: froyer <@t> bitstream.net (Ford Royer) Date: Thu Feb 21 08:26:13 2008 Subject: [Histonet] Blair or other used equipment resources? In-Reply-To: <01a201c8741b$7371dc80$6601a8c0@FSROGER> References: <47BC9DA3.7040103@umdnj.edu> <01a201c8741b$7371dc80$6601a8c0@FSROGER> Message-ID: <002e01c87495$a5788fb0$7701a80a@Ford> Hi Cheryl, My contact information is listed below. How may I help you? ~ Ford Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL:? 612-839-1046 Phone:? 763-542-8725 Fax:? 763-546-4830 eMail:? froyer@bitstream.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Wednesday, February 20, 2008 5:51 PM To: Histonet@Pathology.swmed.edu Cc: Histonet@Pathology.swmed.edu Subject: [Histonet] Blair or other used equipment resources? Hi--could someone help with a phone number for used/refurbished equipment companies? There is a fellow (Mr. Royer?) who posts sometimes? Help? Thank you! Cheryl Full Staff Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JSCHUMA1 <@t> Fairview.org Thu Feb 21 08:27:25 2008 From: JSCHUMA1 <@t> Fairview.org (Schumacher, Jennifer J) Date: Thu Feb 21 08:27:43 2008 Subject: [Histonet] Blair or other used equipment resources? In-Reply-To: <01a201c8741b$7371dc80$6601a8c0@FSROGER> Message-ID: Ford Royer Histology/Pathology Sales Minnesota Medical Specialists Phone 763-542-8725 Toll free 888-790-9686 Fax 763-546-4830 Web: www.minnesotamedical.com Email: clinicallab@minnesotamedical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Wednesday, February 20, 2008 5:51 PM To: Histonet@Pathology.swmed.edu Cc: Histonet@Pathology.swmed.edu Subject: [Histonet] Blair or other used equipment resources? Hi--could someone help with a phone number for used/refurbished equipment companies? There is a fellow (Mr. Royer?) who posts sometimes? Help? Thank you! Cheryl Full Staff Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rydomsal <@t> yahoo.com Thu Feb 21 08:37:44 2008 From: rydomsal <@t> yahoo.com (Ryan Dominique Salazar) Date: Thu Feb 21 08:37:47 2008 Subject: [Histonet] saturated picric acid Message-ID: <103137.56813.qm@web52309.mail.re2.yahoo.com> Hi histopeeps, Please provide a procedure on how to saturate picric acid using the raw chemical picric acid. We just happen to have this raw picric acid in our stock and we'll try to use it for bouin's solution, though we procure saturated picric acid when we prepare the fixative. Thank you very much for any info. Ryan Dominique T. Salazar, RMT, AMT Maccine Pte Ltd, Singapore --------------------------------- Never miss a thing. Make Yahoo your homepage. From rjbuesa <@t> yahoo.com Thu Feb 21 08:42:52 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 21 08:42:59 2008 Subject: [Histonet] Help with rodent tissue In-Reply-To: <276107.88664.qm@web50907.mail.re2.yahoo.com> Message-ID: <857196.49527.qm@web61213.mail.yahoo.com> Cheryl: Rodent tissues are inherently lower in fat and with less water in their fat tissue and this is a "dangerous" combination when processing because, as you point out, the tissues you received are overprocessed so you will have ptoblems sectioning, extending the sections and holding them to your slides. Try ALL the following steps: 1- prepare a solution of 0.2% (v/v) of fabric softener and prepare a frozen block with it. This frozen 0.2% fabric softerner will be used to cold the blocks. Face them off and place them face down over the frozen softener covered with paper towels in a way that the blocks are going to be in contact with the softener for at least 30 minutes before trying to section. 2- increase the temperature of your water bath at 50-52?C, close to the paraffin melting point WITHOUT reaching it. 3- add to your water bath 0.25 mL of liquid detergent (but NOT dish washer). This step, and the previous one are designed to lower the water surface tension and facilitate the sections expansion. 4- add 1 mL of Elmer's (white) glue to the water bath. The water will be slightly whitish but this weak glue solution will help to hold the sections to the slides. When you get to the area in the block that you want to section, cool it with a ice cube wrapped in a gauze and take the sections SLOWLY. This combinaiton should help you, but all the steps together. Good luck with your rodents' blocks! Ren? J. Cheryl wrote: --------------------------------- Never miss a thing. Make Yahoo your homepage. From b-frederick <@t> northwestern.edu Thu Feb 21 08:48:08 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Feb 21 08:48:25 2008 Subject: [Histonet] Dako Info In-Reply-To: Message-ID: <000e01c87498$c8e6d080$d00f7ca5@lurie.northwestern.edu> Why doesn't someone ask their local Dako rep? We have the flex kit and it does work (10 minutes in primary!)The sample kit was HUGE and will last a while. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sally Price Sent: Wednesday, February 20, 2008 5:55 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako Info Nita: I've heard that envision-plus and LSAB2 will be discontinued, and that Dako wants to encourage folks to use a new 2-part polymer system called FLEX -- which is supposed to cost a ton more than EP/EP-DualLink. Anybody have confirmation of this? -- Sally ------------------------------ Message: 8 Date: Wed, 20 Feb 2008 11:13:43 -0600 From: "Nita Searcy" Subject: [Histonet] Dako Info To: histonet@lists.utsouthwestern.edu Posting for Lead Immunohistochemistry Technologist: Anyone using Dako Envision & Detection system heard about it being discontinued? What substitutions are you considering? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> u.arizona.edu Thu Feb 21 09:03:32 2008 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Thu Feb 21 09:11:04 2008 Subject: [Histonet] Help with rodent tissue In-Reply-To: <857196.49527.qm@web61213.mail.yahoo.com> References: <276107.88664.qm@web50907.mail.re2.yahoo.com> <857196.49527.qm@web61213.mail.yahoo.com> Message-ID: <6.2.3.4.1.20080221080247.01ef1cf8@algranth.inbox.email.arizona.edu> Cheryl, I do a lot of rodent tissue here and even though I process it myself occasionally there are some that are pretty dried out and need soaking. A few months ago there was a discussion on histonet about putting glycerin in the water that you soak your faced off blocks in. I tried this and it works wonderfully. We don't measure out the glycerin although I think if you go back in the histonet archives you will find the actual "recipe". We just put a small amount of glycerin into a 500 ml bottle and fill it up with DI water - shake and soak. And I have noticed that my fingertips are now softer too! Andi Grantham At 07:42 AM 2/21/2008, Rene J Buesa wrote: >Cheryl: > Rodent tissues are inherently lower in fat > and with less water in their fat tissue and > this is a "dangerous" combination when > processing because, as you point out, the > tissues you received are overprocessed so you > will have ptoblems sectioning, extending the > sections and holding them to your slides. > Try ALL the following steps: > 1- prepare a solution of 0.2% (v/v) of fabric > softener and prepare a frozen block with it. > This frozen 0.2% fabric softerner will be used > to cold the blocks. Face them off and place > them face down over the frozen softener covered > with paper towels in a way that the blocks are > going to be in contact with the softener for at > least 30 minutes before trying to section. > 2- increase the temperature of your water > bath at 50-52?C, close to the paraffin melting point WITHOUT reaching it. > 3- add to your water bath 0.25 mL of liquid > detergent (but NOT dish washer). This step, and > the previous one are designed to lower the > water surface tension and facilitate the sections expansion. > 4- add 1 mL of Elmer's (white) glue to the > water bath. The water will be slightly whitish > but this weak glue solution will help to hold the sections to the slides. > > When you get to the area in the block that > you want to section, cool it with a ice cube > wrapped in a gauze and take the sections SLOWLY. > > This combinaiton should help you, but all the steps together. > Good luck with your rodents' blocks! >Ren? J. > >Cheryl wrote: > > > > > > >--------------------------------- >Never miss a thing. Make Yahoo your homepage. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From mickie25 <@t> netzero.net Thu Feb 21 09:23:49 2008 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Thu Feb 21 09:25:01 2008 Subject: [Histonet] Blair or other used equipment resources? In-Reply-To: <01a201c8741b$7371dc80$6601a8c0@FSROGER> References: <47BC9DA3.7040103@umdnj.edu> <01a201c8741b$7371dc80$6601a8c0@FSROGER> Message-ID: Belair Instrument Company, Springfield, NJ 800-783-9424 Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Wednesday, February 20, 2008 3:51 PM To: Histonet@Pathology.swmed.edu Cc: Histonet@Pathology.swmed.edu Subject: [Histonet] Blair or other used equipment resources? Hi--could someone help with a phone number for used/refurbished equipment companies? There is a fellow (Mr. Royer?) who posts sometimes? Help? Thank you! Cheryl Full Staff Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Feb 21 09:34:38 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 21 09:34:52 2008 Subject: [Histonet] saturated picric acid In-Reply-To: <103137.56813.qm@web52309.mail.re2.yahoo.com> Message-ID: <436669.60945.qm@web61216.mail.yahoo.com> Ryan: Picric acid solubility is 0.7% (g/mL of distilled water) so any amount above that will make an oversaturated solution. Most recipes recommend 1.2 to 1.4 g/100 mL of DW You should have always some amount of undissolved picric acid in the bottom of your stock (oversaturated) solution and use the supernatant to assure a saturated solution always. Additionally you should NEVER have dried picric acid on your shelf because it can explote. ALWAYS add water to your "pure" picric acid to avoid ignition or explosion to shock. Ren? J. Ryan Dominique Salazar wrote: Hi histopeeps, Please provide a procedure on how to saturate picric acid using the raw chemical picric acid. We just happen to have this raw picric acid in our stock and we'll try to use it for bouin's solution, though we procure saturated picric acid when we prepare the fixative. Thank you very much for any info. Ryan Dominique T. Salazar, RMT, AMT Maccine Pte Ltd, Singapore --------------------------------- Never miss a thing. Make Yahoo your homepage. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From jennifer.bull <@t> northwestpathology.com Thu Feb 21 09:45:39 2008 From: jennifer.bull <@t> northwestpathology.com (Bull, Jennifer L.) Date: Thu Feb 21 09:46:25 2008 Subject: [Histonet] Dako Autostainer Plus vs. Thermo Fisher Autostainer Message-ID: <719B46988560834BBE7D7F72EEB7C10A01853405@HINET1.hinet.org> I'm looking for some feedback on the Dako Autostainer Plus vs. the Thermo Fisher (LabVision) Autostainer. I know they are basically the same instrument with a few upgrades that Dako added, but does anyone have any feedback as far as usability, maintenance, software etc.... Thanks in Advance! Jennifer Bull jennifer.bull@northwestpathology.com From jennifer.bull <@t> northwestpathology.com Thu Feb 21 09:56:29 2008 From: jennifer.bull <@t> northwestpathology.com (Bull, Jennifer L.) Date: Thu Feb 21 09:57:27 2008 Subject: [Histonet] RE: Dako Info Message-ID: <719B46988560834BBE7D7F72EEB7C10A01853406@HINET1.hinet.org> I have confirmed with our local Rep that Dako is no longer going to carry the Envision. If you are a current customer with a contract with Dako, they will continue to supply this detection, but they are moving to the new system and Flex Plus which is more expensive. They are selling it as a package with all your ancillaries based on $8 to $8.50/slide. (Almost as costly as Ventana now...) As far as substitutes go, BioCare sells a Mach 4 detection that is a dual polymer 2-step system that applies a mouse probe and follows with a rabbit polymer. It is supposed to me more sensitive than even the Flex. We will be trying it in the coming week. Hope this helps! Jennifer Bull jennifer.bull@northwestpathology.com From ploykasek <@t> phenopath.com Thu Feb 21 10:18:46 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Thu Feb 21 10:18:58 2008 Subject: FW: [Histonet] Dako Info In-Reply-To: <8B07D141BCDE434285DC12B3290E3FB301CCCDC7@exbackca.caus.dako.net> Message-ID: Perhaps this will help clear up some of the confusion. Patti Loykasek ------ Forwarded Message From: "DAKOTechServ" Date: Wed, 20 Feb 2008 10:55:15 -0800 To: "Patti Loykasek" Subject: RE: [Histonet] Dako Info Hi Patti: To clear up any questions about Dako EnVision products: The EnVision kit (standard sensitivity) has been discontinued. The EnVision+ kits are still available and are not being discontinued. If you would like to post this information, feel free. With regards, Karen Nicolaisen Atwood MT(ASCP) CLS Dako Technical Support -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Wednesday, February 20, 2008 9:28 AM To: Nita Searcy; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Dako Info I think I recall hearing that the Envision, not Envision+, was being discontinued, but I am not 100% positive. Patti Loykasek > Posting for Lead Immunohistochemistry Technologist: > > Anyone using Dako Envision & Detection system heard about it being > discontinued? What substitutions are you considering? > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------ End of Forwarded Message This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From asmith <@t> mail.barry.edu Thu Feb 21 10:23:18 2008 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Thu Feb 21 10:23:27 2008 Subject: [Histonet] saturated picric acid In-Reply-To: <103137.56813.qm@web52309.mail.re2.yahoo.com> Message-ID: I make saturated aqueous picric acid by adding 1.5 g wet picric acid to 100 ml distilled water and stirring overnight. This is an excess of ~0.1 g. Note that picric acid is shipped wet and absolutely must stay wet. Dry picric acid is very, very dangerous! Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ryan Dominique Salazar Sent: Thursday, February 21, 2008 9:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] saturated picric acid Hi histopeeps, Please provide a procedure on how to saturate picric acid using the raw chemical picric acid. We just happen to have this raw picric acid in our stock and we'll try to use it for bouin's solution, though we procure saturated picric acid when we prepare the fixative. Thank you very much for any info. Ryan Dominique T. Salazar, RMT, AMT Maccine Pte Ltd, Singapore --------------------------------- Never miss a thing. Make Yahoo your homepage. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Thu Feb 21 10:52:41 2008 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Feb 21 10:53:05 2008 Subject: [Histonet] Buffer for ISH Message-ID: <47BD6609020000770000AE0F@gwmail4.harthosp.org> I am posting this question for a friend that works in veterinary medicine: "is there a commercially available hybridization buffer for ISH that can be purchased separately (or as part of a kit)?" Thanks, Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From liz <@t> premierlab.com Thu Feb 21 11:27:44 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Feb 21 11:27:56 2008 Subject: [Histonet] Buffer for ISH In-Reply-To: <4270C9D4F89B4C8EB2835A4D1DC3D85D@PremierLab.local> References: <4270C9D4F89B4C8EB2835A4D1DC3D85D@PremierLab.local> Message-ID: Richard I was looking for one too, but I could not locate one. We prepared our own. We buy alot of our probes from Genedetect and they were telling me that in the future they may provide an hybidization buffer. I use theirs, it takes about 2 days to make because of the high concentration of dextran sulfate. Teri Johnson sent me one that looked a bit simpler. I have a SOP for the one I use if you are interested. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, February 21, 2008 10:00 AM To: Histonet Subject: [Histonet] Buffer for ISH I am posting this question for a friend that works in veterinary medicine: "is there a commercially available hybridization buffer for ISH that can be purchased separately (or as part of a kit)?" Thanks, Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Thu Feb 21 12:31:23 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Thu Feb 21 12:31:29 2008 Subject: [Histonet] Re: HistoBath Message-ID: Vicki Gauch asks: >>Our isopentane bath (Histo Bath) that we use to freeze our frozen section blocks has finally stopped working and cannot be repaired. We tried to order a replacement unit only to find out that they no longer make it !!! The company also did not have another option. Does anyone know where we can get something like this?<< Molotov cocktail, you mean! We used acetone in ours. The fire and explosion hazard of this device is unacceptable. I found a suitable fluorocarbon substitute, but hissy-fits were thrown when I suggested the change. We actually replaced the unit about two years ago - I won't say where I was then.. It took several months to get a new one Made in China. I never succeeded in finding another user of the thing, and I don't wonder why. If anybody's interested in the fluorocarbon substitute for acetone or isopentane, I can look it up. Meanwhile, your pathologists can freeze tissue like everybody else does. Bob Richmond Samurai Pathologist Knoxville TN From SCOTT.TURNER <@t> SPCORP.COM Thu Feb 21 13:22:57 2008 From: SCOTT.TURNER <@t> SPCORP.COM (Turner, Scott) Date: Thu Feb 21 13:23:07 2008 Subject: [Histonet] Molecular Probes Zenon labeling kits In-Reply-To: <2884B897182A1D438C7BA24B9A8F94A22674BA@hqsvr01mail.cgi.com> Message-ID: <9A919A5D70313A4D9C56A025710874080313D690@kenmsg40.us.schp.com> I have used these kits quite extensively and found them to be very good, especially for multiplexed assays with two (or more) antibodies from the same species. I have found that incubating the labeled Fab fragment with the primary antibody for longer than 5 min (as recommended in the Molecular Probes protocol) generally produces better results (I typically incubate for 30 min before adding the blocking Ig). It may also be necessary to increase the molar ratio from 1:3 to 1:6 for some low affinity antibodies to amplify the weak signal. It is my general impression from having used a lot of their products that Molecular Probes makes high quality reagents that work quite well. Scott Turner Schering-Plough Biopharma 901 California Ave Palo Alto, CA 94304 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Melissa Gonzalez Sent: Wednesday, February 20, 2008 05:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Molecular Probes Zenon labeling kits Has anyone used the labeling kits (Zenon) from Invitrogen to directly label their abs? How well do they work? They show fabulous examples online of cell staining, but I was wondering how practical they were for tissue staining and epifluorescence detection. Thanks, Melissa Melissa A. Gonz?lez Edick R&D, Cell Genesys Inc. 500 Forbes Blvd South San Francisco, CA 94080 p(650) 266-3168 f (650) 266-3080 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From Heather.D.Renko <@t> osfhealthcare.org Thu Feb 21 13:47:31 2008 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Thu Feb 21 13:47:42 2008 Subject: [Histonet] RE: picric acid Message-ID: <40026EDDE64CDA47AB382C52619ACD3C0A09DD76@pmc-rfd-mx01.intranet.osfnet.org> As Frieda tells: The solubility of picric acid is 1.23g/100mL of water. Some picric acid should remain undissolved. Hope this helps and Friday is just around the corner! Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From Allison_Scott <@t> hchd.tmc.edu Thu Feb 21 14:04:53 2008 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Thu Feb 21 14:07:00 2008 Subject: [Histonet] PI Reports/Productivity Message-ID: <1872B4A455B7974391609AD8034C79FC8BD3F9@LBEXCH01.hchd.local> Hello to everyone. I am interested in what PI reports are being done in histology besides frozen section TAT and specimen discrepancy. Does anyone measure productivity. Our director mentioned it in a supervisors meeting. How is it done. Any help would be appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas From Sharon.Davis-Devine <@t> carle.com Thu Feb 21 14:14:47 2008 From: Sharon.Davis-Devine <@t> carle.com (Sharon.Davis-Devine) Date: Thu Feb 21 14:14:53 2008 Subject: [Histonet] CAP guidelines Message-ID: <44780C571F28624DBB446DE55C4D733A021E08D9@EXCHANGEBE1.carle.com> Ok, Histonetters, got another burning question for you. I have heard that there is a new CAP guideline that states that there needs to be two patient identifiers on the tissue block. We have a block printer and we all know that there is very limited space on the block for much info, so if we need to have two patient identifiers things will get very tight. For those of you who have block printers, how are you handling this? All info and suggestions are greatly appreciated. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com From CIngles <@t> uwhealth.org Thu Feb 21 14:22:43 2008 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Thu Feb 21 14:22:49 2008 Subject: [Histonet] HistoBath References: Message-ID: <08A0A863637F1349BBFD83A96B27A50A1200EF@uwhis-xchng3.uwhis.hosp.wisc.edu> Vicki: I work in a Mohs clinic (frozen skins) we love our liquid nitrogen guns. We have a large tank to store it, and fill the guns every morning. I like them alot better than the isopentane bath we used to use. Particularly when processing fatty specimens. We freeze our tissue in the cryostat initially, then use the cryogun when there are areas that need to be colder to section. The advantage is that you can get very specific in the area you want to freeze, so the OCT, etc. won't get so cold it shatters, like wise with the non-fat elements of the tissue section. Liquid Nitrogen is also alot safer than isopentane in the lab. If you are interested in these, let me know, I'll get you more detailed info. Claire ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Vicki Gauch Sent: Thu 2/21/2008 8:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HistoBath Hi everyone, Our isopentane bath (Histo Bath) that we use to freeze our frozen section blocks has finally stopped working and cannot be repaired. We tried to order a replacement unit only to find out that they no longer make it !!! The company also did not have another option. Does anyone know where we can get something like this? Any help would be greatly appreciated.. Thanks, Vicki Gauch AMCH Albany, NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Thu Feb 21 14:42:11 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Feb 21 14:43:06 2008 Subject: [Histonet] CAP guidelines In-Reply-To: <44780C571F28624DBB446DE55C4D733A021E08D9@EXCHANGEBE1.carle.com> References: <44780C571F28624DBB446DE55C4D733A021E08D9@EXCHANGEBE1.carle.com> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82B37@EMAIL.archildrens.org> I don't see this in the guidelines...does someone have a checklist number for what Sharon is asking below? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Thursday, February 21, 2008 2:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP guidelines Ok, Histonetters, got another burning question for you. I have heard that there is a new CAP guideline that states that there needs to be two patient identifiers on the tissue block. We have a block printer and we all know that there is very limited space on the block for much info, so if we need to have two patient identifiers things will get very tight. For those of you who have block printers, how are you handling this? All info and suggestions are greatly appreciated. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Thu Feb 21 14:47:58 2008 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Thu Feb 21 14:49:14 2008 Subject: [Histonet] CAP guidelines In-Reply-To: <44780C571F28624DBB446DE55C4D733A021E08D9@EXCHANGEBE1.carle.com> Message-ID: This is from the Sep 2007 checklist. ANP.21100 Phase II N/A YES NO Are blocks identified adequately? NOTE: Each block of tissue must be identified by the entire accession number assigned to the case and by any descriptive letter(s)/number(s) added by the prosector during the dissection. If additional blocks are prepared later, all lists and logs must reflect these additions. Identification number and letter(s)/numbers(s) must be affixed to all blocks in a manner that remains legible. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Thursday, February 21, 2008 3:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP guidelines Ok, Histonetters, got another burning question for you. I have heard that there is a new CAP guideline that states that there needs to be two patient identifiers on the tissue block. We have a block printer and we all know that there is very limited space on the block for much info, so if we need to have two patient identifiers things will get very tight. For those of you who have block printers, how are you handling this? All info and suggestions are greatly appreciated. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hhernandez <@t> pathreflab.com Thu Feb 21 15:00:38 2008 From: hhernandez <@t> pathreflab.com (Hector Hernandez) Date: Thu Feb 21 14:58:26 2008 Subject: [Histonet] CAP guidelines Message-ID: I think everyone in the Histo world already does this. Example S1000-07 A1, A2, etc. No need to get excited about this question. The two identifiers are the surgical# and any sub letter or number. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Thursday, February 21, 2008 2:48 PM To: 'Sharon.Davis-Devine'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP guidelines This is from the Sep 2007 checklist. ANP.21100 Phase II N/A YES NO Are blocks identified adequately? NOTE: Each block of tissue must be identified by the entire accession number assigned to the case and by any descriptive letter(s)/number(s) added by the prosector during the dissection. If additional blocks are prepared later, all lists and logs must reflect these additions. Identification number and letter(s)/numbers(s) must be affixed to all blocks in a manner that remains legible. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Thursday, February 21, 2008 3:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP guidelines Ok, Histonetters, got another burning question for you. I have heard that there is a new CAP guideline that states that there needs to be two patient identifiers on the tissue block. We have a block printer and we all know that there is very limited space on the block for much info, so if we need to have two patient identifiers things will get very tight. For those of you who have block printers, how are you handling this? All info and suggestions are greatly appreciated. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Thu Feb 21 15:06:03 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Feb 21 15:07:07 2008 Subject: [Histonet] Buffer for ISH Message-ID: Try Sigma: Fluka Cat No 53754 (2x Hybridisation Solution) Contains: 10x SSC 10x Denhardt's Solution 200ug/ml Salmon Testes DNA pH 6.9 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, 22 February 2008 3:53 AM To: Histonet Subject: [Histonet] Buffer for ISH I am posting this question for a friend that works in veterinary medicine: "is there a commercially available hybridization buffer for ISH that can be purchased separately (or as part of a kit)?" Thanks, Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Thu Feb 21 15:08:59 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Feb 21 15:09:55 2008 Subject: [Histonet] CAP guidelines Message-ID: Wouldn't two identifiers be eg Accession Number and patient surname (or MRN)? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hector Hernandez Sent: Friday, 22 February 2008 8:01 AM To: 'Douglas D Deltour'; 'Sharon.Davis-Devine'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP guidelines I think everyone in the Histo world already does this. Example S1000-07 A1, A2, etc. No need to get excited about this question. The two identifiers are the surgical# and any sub letter or number. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Thursday, February 21, 2008 2:48 PM To: 'Sharon.Davis-Devine'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP guidelines This is from the Sep 2007 checklist. ANP.21100 Phase II N/A YES NO Are blocks identified adequately? NOTE: Each block of tissue must be identified by the entire accession number assigned to the case and by any descriptive letter(s)/number(s) added by the prosector during the dissection. If additional blocks are prepared later, all lists and logs must reflect these additions. Identification number and letter(s)/numbers(s) must be affixed to all blocks in a manner that remains legible. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Thursday, February 21, 2008 3:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP guidelines Ok, Histonetters, got another burning question for you. I have heard that there is a new CAP guideline that states that there needs to be two patient identifiers on the tissue block. We have a block printer and we all know that there is very limited space on the block for much info, so if we need to have two patient identifiers things will get very tight. For those of you who have block printers, how are you handling this? All info and suggestions are greatly appreciated. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From doug <@t> ppspath.com Thu Feb 21 15:20:35 2008 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Thu Feb 21 15:21:40 2008 Subject: SPAM-LOW: RE: [Histonet] CAP guidelines Message-ID: I disagree with that interpretation Hector. I do not consider the block number a patient identifier. Still I consider Sharon's original question hearsay until I see it on a CAP checklist. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hector Hernandez Sent: Thursday, February 21, 2008 4:01 PM To: 'Douglas D Deltour'; 'Sharon.Davis-Devine'; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: RE: [Histonet] CAP guidelines I think everyone in the Histo world already does this. Example S1000-07 A1, A2, etc. No need to get excited about this question. The two identifiers are the surgical# and any sub letter or number. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Thursday, February 21, 2008 2:48 PM To: 'Sharon.Davis-Devine'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP guidelines This is from the Sep 2007 checklist. ANP.21100 Phase II N/A YES NO Are blocks identified adequately? NOTE: Each block of tissue must be identified by the entire accession number assigned to the case and by any descriptive letter(s)/number(s) added by the prosector during the dissection. If additional blocks are prepared later, all lists and logs must reflect these additions. Identification number and letter(s)/numbers(s) must be affixed to all blocks in a manner that remains legible. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Thursday, February 21, 2008 3:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP guidelines Ok, Histonetters, got another burning question for you. I have heard that there is a new CAP guideline that states that there needs to be two patient identifiers on the tissue block. We have a block printer and we all know that there is very limited space on the block for much info, so if we need to have two patient identifiers things will get very tight. For those of you who have block printers, how are you handling this? All info and suggestions are greatly appreciated. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Thu Feb 21 15:30:28 2008 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Thu Feb 21 15:31:20 2008 Subject: [Histonet] CAP guidelines In-Reply-To: Message-ID: If this ever does come to be down the road it would most likely be the accession number and a 2D barcode. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Thursday, February 21, 2008 4:09 PM To: Hector Hernandez; Douglas D Deltour; Sharon.Davis-Devine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP guidelines Wouldn't two identifiers be eg Accession Number and patient surname (or MRN)? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hector Hernandez Sent: Friday, 22 February 2008 8:01 AM To: 'Douglas D Deltour'; 'Sharon.Davis-Devine'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP guidelines I think everyone in the Histo world already does this. Example S1000-07 A1, A2, etc. No need to get excited about this question. The two identifiers are the surgical# and any sub letter or number. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Thursday, February 21, 2008 2:48 PM To: 'Sharon.Davis-Devine'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP guidelines This is from the Sep 2007 checklist. ANP.21100 Phase II N/A YES NO Are blocks identified adequately? NOTE: Each block of tissue must be identified by the entire accession number assigned to the case and by any descriptive letter(s)/number(s) added by the prosector during the dissection. If additional blocks are prepared later, all lists and logs must reflect these additions. Identification number and letter(s)/numbers(s) must be affixed to all blocks in a manner that remains legible. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Thursday, February 21, 2008 3:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP guidelines Ok, Histonetters, got another burning question for you. I have heard that there is a new CAP guideline that states that there needs to be two patient identifiers on the tissue block. We have a block printer and we all know that there is very limited space on the block for much info, so if we need to have two patient identifiers things will get very tight. For those of you who have block printers, how are you handling this? All info and suggestions are greatly appreciated. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Thu Feb 21 15:30:32 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Feb 21 15:31:37 2008 Subject: SPAM-LOW: RE: [Histonet] CAP guidelines In-Reply-To: <200802212122.m1LLMWc0001666@securemail3.archildrens.org> References: <200802212122.m1LLMWc0001666@securemail3.archildrens.org> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82B39@EMAIL.archildrens.org> I disagree as well. I don't see it in the guidelines published September 07. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Thursday, February 21, 2008 3:21 PM To: 'Hector Hernandez'; 'Sharon.Davis-Devine'; histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: RE: [Histonet] CAP guidelines I disagree with that interpretation Hector. I do not consider the block number a patient identifier. Still I consider Sharon's original question hearsay until I see it on a CAP checklist. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hector Hernandez Sent: Thursday, February 21, 2008 4:01 PM To: 'Douglas D Deltour'; 'Sharon.Davis-Devine'; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: RE: [Histonet] CAP guidelines I think everyone in the Histo world already does this. Example S1000-07 A1, A2, etc. No need to get excited about this question. The two identifiers are the surgical# and any sub letter or number. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Thursday, February 21, 2008 2:48 PM To: 'Sharon.Davis-Devine'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP guidelines This is from the Sep 2007 checklist. ANP.21100 Phase II N/A YES NO Are blocks identified adequately? NOTE: Each block of tissue must be identified by the entire accession number assigned to the case and by any descriptive letter(s)/number(s) added by the prosector during the dissection. If additional blocks are prepared later, all lists and logs must reflect these additions. Identification number and letter(s)/numbers(s) must be affixed to all blocks in a manner that remains legible. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Thursday, February 21, 2008 3:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP guidelines Ok, Histonetters, got another burning question for you. I have heard that there is a new CAP guideline that states that there needs to be two patient identifiers on the tissue block. We have a block printer and we all know that there is very limited space on the block for much info, so if we need to have two patient identifiers things will get very tight. For those of you who have block printers, how are you handling this? All info and suggestions are greatly appreciated. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From collette2 <@t> mail.llnl.gov Thu Feb 21 15:53:57 2008 From: collette2 <@t> mail.llnl.gov (Nicole Collette) Date: Thu Feb 21 15:46:43 2008 Subject: [Histonet] LacZ and decalcification Message-ID: >Hello, All, > >There seems to be a lot of mystery surrounding this issue, I was >hoping someone might have a definitive answer. I have done lots and >lots of lacZ stains in mice- embryos, adults, all kinds of tissues. >I have followed published protocols that should never have been >published, and have recovered somehow to turn out some great lacZ >stains. > >I have never had samples that survived automatic tissue processing >for paraffin, they end up completely unusable with artefactual lacZ >stain. I have been told that I can process as usual for paraffin >embedding (for tissues that don't need decalcification) as long as I >hand-process the tissue to limit the heat on the tissues. Is this >true? Or was I just doing the automatic processing all wrong?? > >I have done frozen sections, post-stain, which turn out fine, but >only up to E17.5 mouse embryos. I have done stain on slides after >sectioning on a few sections of adult bone, undecalcified, using the >CryoJane system, also with the stain working, but it has its >limitations, in that I'd like thicker sections to take better >photos, but they don't stick to the slides very well, since only >very thin works, even with the extra adhesive slides for hard >tissue. Thus, I am back at the decalcification issue. > >Can I decalcify with EDTA before stain? How long for adult mouse >bones, and what percentage of EDTA? I assume the pH is the most >important part of this issue, but I am worried about prolonged >decalcification with the limited fixation allowed for LacZ staining >to avoid osteoclast staining artefacts, and/or overfixation to >survive the long EDTA process (Will it kill the stain? or ruin the >tissue?). I would think the best way would be to stain the bones as >whole-mount tissues, then decalcify (how long and at what >percentage?) after post-fixation, at which point I have fewer >worries. Should I be concerned about stain penetration through >intact bone during stain as whole-mount (dissected free of adherent >tissue, of course). > >Any advice or wisdom would be most helpful. >Thanks so much! >Nicole From STapper <@t> smdc.org Thu Feb 21 16:10:16 2008 From: STapper <@t> smdc.org (Tapper, Sheila J.) Date: Thu Feb 21 16:10:34 2008 Subject: [Histonet] CAP guidelines In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82B39@EMAIL.archildrens.org> Message-ID: I understand the two patient identifiers to be a JCAHO initiative. We are required to use two patient identifiers in the phlebotomy area when collecting blood. The patient is asked their name and birth date. In the Anatomic Pathology area, we are asking that accessioners look not only at the patient name, but also confirm a second identifier, such as their birth date before they accession the case into the LIS. In the case of similar names - it can and has made a difference. Reading the CAP question - I would have to agree with Hector's interpretation. The CAP question is only asking if the block is clearly identified with the accession number and any sub identifiers that tie the block to a specific specimen, in a legible manner that is capable of withstanding the chemicals it will be subjected to. I have worked at labs that would submit 12 blocks of colon with only the accession number on the block, and nothing to identify the individual cassettes. When the doc wanted deepers on a block - he would submit the slide, and the tech would match the slide to the block - very inefficient. That has changed immensely over time - I am showing my age - but I am sure there are still labs that operate that way. Sheila Tapper HT(ASCP) Anatomic Pathology Supervisor SMDC Clinical Laboratory 407 East First Street Duluth, MN 55804 Telephone: 218-786-5472 Fax: 218-786-2369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, February 21, 2008 3:31 PM To: Douglas D Deltour; Hector Hernandez; Sharon.Davis-Devine; histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: RE: [Histonet] CAP guidelines I disagree as well. I don't see it in the guidelines published September 07. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Thursday, February 21, 2008 3:21 PM To: 'Hector Hernandez'; 'Sharon.Davis-Devine'; histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: RE: [Histonet] CAP guidelines I disagree with that interpretation Hector. I do not consider the block number a patient identifier. Still I consider Sharon's original question hearsay until I see it on a CAP checklist. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hector Hernandez Sent: Thursday, February 21, 2008 4:01 PM To: 'Douglas D Deltour'; 'Sharon.Davis-Devine'; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: RE: [Histonet] CAP guidelines I think everyone in the Histo world already does this. Example S1000-07 A1, A2, etc. No need to get excited about this question. The two identifiers are the surgical# and any sub letter or number. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Thursday, February 21, 2008 2:48 PM To: 'Sharon.Davis-Devine'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP guidelines This is from the Sep 2007 checklist. ANP.21100 Phase II N/A YES NO Are blocks identified adequately? NOTE: Each block of tissue must be identified by the entire accession number assigned to the case and by any descriptive letter(s)/number(s) added by the prosector during the dissection. If additional blocks are prepared later, all lists and logs must reflect these additions. Identification number and letter(s)/numbers(s) must be affixed to all blocks in a manner that remains legible. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Thursday, February 21, 2008 3:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP guidelines Ok, Histonetters, got another burning question for you. I have heard that there is a new CAP guideline that states that there needs to be two patient identifiers on the tissue block. We have a block printer and we all know that there is very limited space on the block for much info, so if we need to have two patient identifiers things will get very tight. For those of you who have block printers, how are you handling this? All info and suggestions are greatly appreciated. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From hhernandez <@t> pathreflab.com Thu Feb 21 16:17:37 2008 From: hhernandez <@t> pathreflab.com (Hector Hernandez) Date: Thu Feb 21 16:15:18 2008 Subject: SPAM-LOW: RE: [Histonet] CAP guidelines In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82B39@EMAIL.archildrens.org> Message-ID: Let me put it another way. As Douglas stated on CAP question ANP.21100 Phase II N/A YES NO Are blocks identified adequately? NOTE: Each block of tissue must be identified by the entire accession number assigned to the case and by any descriptive letter(s)/number(s) added by the prosector during the dissection. If additional blocks are prepared later, all lists and logs must reflect these additions. Identification number and letter(s)/numbers(s) must be affixed to all blocks in a manner that remains legible. It doesn't state anything about two identifiers. -----Original Message----- From: Horn, Hazel V [mailto:HornHV@archildrens.org] Sent: Thursday, February 21, 2008 3:31 PM To: Douglas D Deltour; Hector Hernandez; Sharon.Davis-Devine; histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: RE: [Histonet] CAP guidelines I disagree as well. I don't see it in the guidelines published September 07. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Thursday, February 21, 2008 3:21 PM To: 'Hector Hernandez'; 'Sharon.Davis-Devine'; histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: RE: [Histonet] CAP guidelines I disagree with that interpretation Hector. I do not consider the block number a patient identifier. Still I consider Sharon's original question hearsay until I see it on a CAP checklist. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hector Hernandez Sent: Thursday, February 21, 2008 4:01 PM To: 'Douglas D Deltour'; 'Sharon.Davis-Devine'; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: RE: [Histonet] CAP guidelines I think everyone in the Histo world already does this. Example S1000-07 A1, A2, etc. No need to get excited about this question. The two identifiers are the surgical# and any sub letter or number. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Thursday, February 21, 2008 2:48 PM To: 'Sharon.Davis-Devine'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP guidelines This is from the Sep 2007 checklist. ANP.21100 Phase II N/A YES NO Are blocks identified adequately? NOTE: Each block of tissue must be identified by the entire accession number assigned to the case and by any descriptive letter(s)/number(s) added by the prosector during the dissection. If additional blocks are prepared later, all lists and logs must reflect these additions. Identification number and letter(s)/numbers(s) must be affixed to all blocks in a manner that remains legible. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Thursday, February 21, 2008 3:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP guidelines Ok, Histonetters, got another burning question for you. I have heard that there is a new CAP guideline that states that there needs to be two patient identifiers on the tissue block. We have a block printer and we all know that there is very limited space on the block for much info, so if we need to have two patient identifiers things will get very tight. For those of you who have block printers, how are you handling this? All info and suggestions are greatly appreciated. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmahoney <@t> alegent.org Thu Feb 21 16:29:18 2008 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Thu Feb 21 16:32:12 2008 Subject: SPAM-LOW: RE: [Histonet] CAP guidelines In-Reply-To: <20080221212540.D758074806D@mail51-dub.bigfish.com> References: <20080221212540.D758074806D@mail51-dub.bigfish.com> Message-ID: <47BDA6DE0200003C0002C45E@gwia.alegent.org> You can go to the CAP website and pull up the AP checklist. You will see; ANP.21100 Phase II Are blocks identified adequately? NOTE: Each block of tissue must be identified by the entire accession number assigned to the case and by any descriptive letter(s) number(s) added by the prosector during dissection. If additional blocks are prepared later, all lists and los must reflect these additions. Identification number and letter(s) number(s) must be affixed to all blocks in a manner that remains legible. Jan Mahoney Alegent Health Omaha, NE From zodiac29 <@t> comcast.net Thu Feb 21 17:06:57 2008 From: zodiac29 <@t> comcast.net (zodiac29@comcast.net) Date: Thu Feb 21 17:07:04 2008 Subject: [Histonet] unstaining H&E slides Message-ID: <022120082306.29722.47BE041100050B360000741A2212020784C7CD0C0E070B0196@comcast.net> Does anyone have a procedure, with a reference, for unstaining H&E slides? I have seached the internet and looked through my textbooks and have not found a written procedure. I have even consulted the procedure manuel at a local hospital, and they did not have a written procedure. I know that you have to back the slides through the stain rack, omitting the H&E and Eosin, but I'm not sure of the recommended timing in each reagent. Thanks in advance your help From rjbuesa <@t> yahoo.com Thu Feb 21 17:29:38 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 21 17:29:46 2008 Subject: [Histonet] PI Reports/Productivity In-Reply-To: <1872B4A455B7974391609AD8034C79FC8BD3F9@LBEXCH01.hchd.local> Message-ID: <86304.50574.qm@web61213.mail.yahoo.com> Allison: I have been measuring productivity for years and is a managerial tool of utmost importance and relevance not only to calculate supplies but to use in personnel evaluations and crosstraining, as well as to analyze the lab complement nneds. Should be extended to all activities within the histo lab. If you are inetersted in the subject I can send you articles I have written on the subject that you could use to gather your data. Ren? J. "Scott, Allison D" wrote: Hello to everyone. I am interested in what PI reports are being done in histology besides frozen section TAT and specimen discrepancy. Does anyone measure productivity. Our director mentioned it in a supervisors meeting. How is it done. Any help would be appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From susanbachus <@t> verizon.net Thu Feb 21 17:43:31 2008 From: susanbachus <@t> verizon.net (Susan Bachus) Date: Thu Feb 21 17:43:41 2008 Subject: [Histonet] Buffer for ISH References: <4270C9D4F89B4C8EB2835A4D1DC3D85D@PremierLab.local> Message-ID: <001601c874e3$9193a630$2e01a8c0@RESLAPTOP> It only takes a few minutes to make some hybridization buffer up IF you make some 50% dextran sulfate in advance (which does take a LONG time to get into solution!) and keep that in the refrigerator. I've continued to use a large batch of dextran sulfate for years. If you warm the dextran sulfate up for an hour or so in advance (I put it in my hybridization incubator) it really doesn't take long to add the other ingredients for a fresh batch of hybridization buffer for each experiment. The hybridization buffer can be kept for a while (months) in the freezer but ingredients will slowly begin to precipitate out. We've consistently had good results by coasting on the same batch of dextran sulfate as long as it lasts but making the buffer fresh for each assay. Susan ----- Original Message ----- From: "Liz Chlipala" To: "Richard Cartun" ; "Histonet" Sent: Thursday, February 21, 2008 12:27 PM Subject: RE: [Histonet] Buffer for ISH Richard I was looking for one too, but I could not locate one. We prepared our own. We buy alot of our probes from Genedetect and they were telling me that in the future they may provide an hybidization buffer. I use theirs, it takes about 2 days to make because of the high concentration of dextran sulfate. Teri Johnson sent me one that looked a bit simpler. I have a SOP for the one I use if you are interested. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, February 21, 2008 10:00 AM To: Histonet Subject: [Histonet] Buffer for ISH I am posting this question for a friend that works in veterinary medicine: "is there a commercially available hybridization buffer for ISH that can be purchased separately (or as part of a kit)?" Thanks, Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> Stowers-Institute.org Thu Feb 21 17:48:07 2008 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Thu Feb 21 17:48:24 2008 Subject: [Histonet] Re: LacZ and decalcification Message-ID: Nicole, wow! What a well informed question, well done! I don't know everything there is to know about B-gal and x-gal staining. I can only give you advice based on my experience. You may well have more of it than me. Having said all that, we've tried to stay close to the original x-gal protocol we saw from Lobe etal. using Z/EG mice. Their fixative was 0.2% glutaraldehyde in PBS buffer with EGTA and MgCl added. Larger samples were fixed in 0.2% glutaraldehyde with 2% PFA in the above buffer. All were fixed on ice, with shaking. Fixation was minimal, less than 4 hours for the largest samples. We have never tried to do x-gal staining on paraffin embedded samples. We only do them on cryosections and whole mounts. If we do whole mounts, we post-fix overnight (or longer depending on the sample), and do paraffin sections (8 microns thick) to demonstrate the activity. You have every right to be worried about bone samples, decalcification, and fixation. We have approached these samples in two ways and have had success with both. One technique is from a journal article in J Bone and Mineral Research, Vol. 20, Num. 7, 2005 (Hens, etal). Here is their protocol (copy/pasted verbatim from the article), which we followed except we used 10% EDTA. The results were good, and the decal only took 3 days (we checked it with faxitron). Your mileage may vary depending on the age, strain, and genetic background of your mice. >>Adult bones were fixed in 2% paraformaldehyde and 0.02% glutaraldehyde in PBS for 1 h at room temperature and washed twice in PBS. Bones were first decalcified in 4% EDTA for 17 days and then washed in PBS for 3 h before being incubated in 0.1% 4-chloro-5-bromo-3-indolyl -D-galactopyranoside (X-gal), 2 mM MgCl2, 5 mM EGTA, 0.02% Nonidet P-40, 5 mM K3Fe(CN)6, and 5 mM K4Fe(CN)6?3 H2O at 30?C overnight. Bones were subsequently washed once with PBS and then postfixed in 4% paraformaldehyde at 4?C overnight. Individual bones were rinsed in 70% ethanol, embedded in paraffin wax, sectioned, and counterstained with eosin.<< You'll notice the gluaraldehyde concentration is very low. I can't imagine it has that much effect since it takes so long to penetrate tissues. They do cut their bones in half to allow better penetration. You will also notice they fix at room temperature (my heroes!). The next technique involves whole mount staining first, and then post-fixation and decal in formic acid (Immunocal). When we first tried using EDTA post-staining, all the blue stain dissolved. We found that if we use formic acid instead, it preserves the x-gal staining. Again, the bones are opened (in the middle if interested in the ends of the bone, or in one end of the bone if interested in the middle) and fixed for a couple hours, then rinsed, then stained overnight with x-gal reagent. Post-fixed, decalcified, then paraffin processed, sectioned and counterstained with Nuclear Fast Red. I hope this helps! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From PMonfils <@t> Lifespan.org Thu Feb 21 18:17:37 2008 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Feb 21 18:17:44 2008 Subject: [Histonet] unstaining H&E slides In-Reply-To: <022120082306.29722.47BE041100050B360000741A2212020784C7CD0C0E070B0196@comcast.net> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273D26@LSRIEXCH1.lsmaster.lifespan.org> I don't have a reference, but I can share my technique. First you have to get the coverslips off, which could take anywhere from a half hour to a couple of days in xylene, depending on how long the slides have been coverslipped. Once the coverslips are off, a half hour in fresh xylene to make sure all traces of old dried mounting medium are removed. Then a couple of rinses in absolute ethanol to remove the xylene. 1 minute in each is plenty, even less if you agitate the slide rack constantly. A similar rinse in 95% alcohol. Then into tap water. Running tap water will remove the eosin within 5 minutes or so, unless you happen to live in an area where the tap water is neutral or slightly acidic. In most areas tap water is slightly basic. A couple of drops of ammonium hydroxide in a staining dish of tap water will remove the eosin much faster, less than 30 seconds. Then I use acid alcohol (1% HCl in 70% ethanol) to remove the hematoxylin. This can take up to 15 minutes or so to get all the stain out, though most of it will be removed within 5 minutes. Also, you can double the HCl concentration to speed things up. Then rinse well in tap water, followed by distilled water if your staining technique calls for it, and restain with your method of choice. From daliaelrouby <@t> hotmail.com Thu Feb 21 23:47:53 2008 From: daliaelrouby <@t> hotmail.com (Dalia El Rouby) Date: Thu Feb 21 23:47:58 2008 Subject: [Histonet] (no subject) Message-ID: Dear all: It seems that someone got access to my e-mail and used by name to send message. I apologize for that and inform you that my account on yahoo: daliaelrouby@yahoo.com is no longer valid as someone else uses it and changed my password _________________________________________________________________ Express yourself instantly with MSN Messenger! Download today it's FREE! http://messenger.msn.click-url.com/go/onm00200471ave/direct/01/ From abright <@t> brightinstruments.com Fri Feb 22 05:25:45 2008 From: abright <@t> brightinstruments.com (Alan Bright) Date: Fri Feb 22 05:56:17 2008 Subject: [Histonet] HistoBath References: Message-ID: Dear Vicki, We manufacture the Clini-RF Rapid Freezer for the purpose you require, also it will operate down to -80 degs C, which is lower than the Histobath. There are options available for this model with respect to temperature control and timers, we have also for special applications manufacture models to go down below -100 degs C. I hope this is of interest. Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype: dazzle0 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vicki Gauch Sent: 21 February 2008 14:11 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HistoBath Hi everyone, Our isopentane bath (Histo Bath) that we use to freeze our frozen section blocks has finally stopped working and cannot be repaired. We tried to order a replacement unit only to find out that they no longer make it !!! The company also did not have another option. Does anyone know where we can get something like this? Any help would be greatly appreciated.. Thanks, Vicki Gauch AMCH Albany, NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NSEARCY <@t> swmail.sw.org Fri Feb 22 06:21:51 2008 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Fri Feb 22 06:22:13 2008 Subject: [Histonet] Two Identifiers Message-ID: The statement is from the Lab General question .40490- see the "note" attached to the interpretation in which it specifically states"Personnel must confirm the patient's identity by checking at least two identifiers before collecting a specimen." I interpret that to be "out of my circle of influence" as far as biopsies, fluids, etc- unless I am collecting, then I verify identity. Also JCAHO Provision of Care -PC.5.10 as well as CLSI document H3 calls for two identifiers- "when administering medications or blood products; taking blood samples and other samples for clinical testing, or providing any other treatments or procedures." Courtesy of my compliance officer. But, as you know, inspectors can interpret however they choose and you have a right to respond. From Joanne.Malinowski <@t> crl.com Fri Feb 22 06:46:41 2008 From: Joanne.Malinowski <@t> crl.com (Malinowski, Joanne O,) Date: Fri Feb 22 06:47:03 2008 Subject: [Histonet] Polycut Microtome Message-ID: <3E94391DD24ECE418DCBA5EF74BA299453C658@shr-exch1.na01.crl.com> Hello Plastics People, Can anyone direct me to a used Polycut Leica SM2500? Most likely for parts, but open for options for the right price! Thank you in advance for your help once again, Joanne Joanne Malinowski,HT ASCP Plastics Lab Manager, Medical Devices Division Charles River Laboratories Pathology Associates 15 Worman's Mill Court, Suite I Frederick, Maryland 21701 Phone 301-624-2034 Fax 301-663-8994 Email: joanne.malinowski@us.crl.com From rjbuesa <@t> yahoo.com Fri Feb 22 07:09:56 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 22 07:10:02 2008 Subject: [Histonet] unstaining H&E slides In-Reply-To: <022120082306.29722.47BE041100050B360000741A2212020784C7CD0C0E070B0196@comcast.net> Message-ID: <412800.74506.qm@web61212.mail.yahoo.com> It is very simple: just hydrate your sections and place them in 0.1% hydrochloric acid aq.sol. until they are completely destained. Wash them in a mild alkaline solution (to neutralize the effects of the acid), wash thoroughly in water and dist. water and restain them with any new procedure. Ren? J. zodiac29@comcast.net wrote: Does anyone have a procedure, with a reference, for unstaining H&E slides? I have seached the internet and looked through my textbooks and have not found a written procedure. I have even consulted the procedure manuel at a local hospital, and they did not have a written procedure. I know that you have to back the slides through the stain rack, omitting the H&E and Eosin, but I'm not sure of the recommended timing in each reagent. Thanks in advance your help _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From christiegowan <@t> msn.com Fri Feb 22 07:17:04 2008 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Fri Feb 22 07:17:15 2008 Subject: [Histonet] PI Reports/Productivity Message-ID: Hello everyone, Allison Scott wrote: I am interested in what PI reports are being done in >histology besides frozen section TAT and specimen discrepancy. Does >anyone measure productivity. Our director mentioned it in a supervisors >meeting. How is it done. >Any help would be appreciated Allison, We measure TAT for Stat Biopsies (out by 6:30) and routine TAT (out by 8:00 and out by 10:00). We have an application through our hospital software that allows me to do this daily. Our process is such that we validate each case in the computer as it is turned out to the Pathologist. At the end of the month I submit a report that shows how many blocks were embedded and stained H&E and what percentage met the turnaround times. I also measure number of special stains and turnaround times but I do this manually. Our hospital uses Cerner Millennium software. Hope this gives you some idea and if you need further info, I would be happy to discuss it with you. Thanks, Christie Gowan Histology Supervisor UAB Medical Center Hospital Birmingham, AL From b-frederick <@t> northwestern.edu Fri Feb 22 07:50:14 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Feb 22 07:50:30 2008 Subject: [Histonet] CAP guidelines In-Reply-To: <44780C571F28624DBB446DE55C4D733A021E08D9@EXCHANGEBE1.carle.com> Message-ID: <000001c87559$dcacf240$d00f7ca5@lurie.northwestern.edu> I have not seen the guideline, but we have a block etcher that holds 3 lines (max)of print. We get all sorts of info on there. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Thursday, February 21, 2008 2:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP guidelines Ok, Histonetters, got another burning question for you. I have heard that there is a new CAP guideline that states that there needs to be two patient identifiers on the tissue block. We have a block printer and we all know that there is very limited space on the block for much info, so if we need to have two patient identifiers things will get very tight. For those of you who have block printers, how are you handling this? All info and suggestions are greatly appreciated. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From arsenn <@t> hsh.org Fri Feb 22 09:13:04 2008 From: arsenn <@t> hsh.org (Senn, Amy R) Date: Fri Feb 22 09:13:09 2008 Subject: [Histonet] (no subject) Message-ID: Hi all... This may be a silly question & I could probably find the answer myself by searching the Internet or our own archives-but I'm allowed only so much time on the computer at work & for the next 2 weeks, I'm on another 'position' so I won't be near the computer at all...So please accept my apologies... We are looking for a 'cheat sheet' for special and immuno stains. We need the name of the stain, the best control tissue, what we're staining (looking) for, and the colors we will see if the stain is done properly. I've searched for this online, but I find SOOO much information packed into tables that it will take me hours to delete what I don't need & enter the information I DO need. I just thought if someone had something like this handy, I could have a copy....if not, I'll just go to the library this weekend and begin making a chart myself from the sticky notes we have all over the lab LOL Also, thank you all who responded to my Calret. question! I received a lot of suggestions, and we're trying them all! Have a great Friday everyone! Amy Histotech, Histology Laboratory Holy Spirit Hospital 503 N. 21st Street Camp Hill, PA 17011 (717) 763-2124 Cell: (724) 494-2237 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You From Sharon.Davis-Devine <@t> carle.com Fri Feb 22 09:35:21 2008 From: Sharon.Davis-Devine <@t> carle.com (Sharon.Davis-Devine) Date: Fri Feb 22 09:36:03 2008 Subject: [Histonet] Two patient identifier question Message-ID: <44780C571F28624DBB446DE55C4D733A021E08E5@EXCHANGEBE1.carle.com> Dear Histonetters, regarding my previous question about the requirement of two patient identifiers on tissue blocks, my information was incorrect. This comes from JACHO not from CAP. Sorry, it always helps to have the correct info before posting a question. So now that JACHO requires this, those of you that have to follow these regulations, how are you handling it? Thanks a bunch. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com From failm <@t> musc.edu Fri Feb 22 09:41:21 2008 From: failm <@t> musc.edu (Fail, Mildred M.) Date: Fri Feb 22 09:40:49 2008 Subject: [Histonet] (chest sheet) Message-ID: Amy , Each special stain should have the results lised for the procedure. As for a "cheat Sheet", you need one specific for your lab. If you have request forms you can do it quickly, by printing beside each stain which control you need, effective but messy and not very professional. I worked with a database to list all our Abs. lot nos, titration dates, dilutions, controls etc. A simple query for bench use pulled up ABs. lot, controls, dilutions,and pretreatment. Designed at my home computer than transfered and maintained at work. The lab I worked in had over 100 Abs. No way did I have the time at work to commit to the initial document. It does take time but is well worth the effort. Rena Fail ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R [arsenn@hsh.org] Sent: Friday, February 22, 2008 10:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi all... This may be a silly question & I could probably find the answer myself by searching the Internet or our own archives-but I'm allowed only so much time on the computer at work & for the next 2 weeks, I'm on another 'position' so I won't be near the computer at all...So please accept my apologies... We are looking for a 'cheat sheet' for special and immuno stains. We need the name of the stain, the best control tissue, what we're staining (looking) for, and the colors we will see if the stain is done properly. I've searched for this online, but I find SOOO much information packed into tables that it will take me hours to delete what I don't need & enter the information I DO need. I just thought if someone had something like this handy, I could have a copy....if not, I'll just go to the library this weekend and begin making a chart myself from the sticky notes we have all over the lab LOL Also, thank you all who responded to my Calret. question! I received a lot of suggestions, and we're trying them all! Have a great Friday everyone! Amy Histotech, Histology Laboratory Holy Spirit Hospital 503 N. 21st Street Camp Hill, PA 17011 (717) 763-2124 Cell: (724) 494-2237 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From failm <@t> musc.edu Fri Feb 22 09:43:44 2008 From: failm <@t> musc.edu (Fail, Mildred M.) Date: Fri Feb 22 09:45:03 2008 Subject: [Histonet] (should read cheat) Message-ID: Right now my former boss is LOL because I always forget to spell check ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fail, Mildred M. [failm@musc.edu] Sent: Friday, February 22, 2008 10:41 AM To: Senn, Amy R; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (chest sheet) Amy , Each special stain should have the results lised for the procedure. As for a "cheat Sheet", you need one specific for your lab. If you have request forms you can do it quickly, by printing beside each stain which control you need, effective but messy and not very professional. I worked with a database to list all our Abs. lot nos, titration dates, dilutions, controls etc. A simple query for bench use pulled up ABs. lot, controls, dilutions,and pretreatment. Designed at my home computer than transfered and maintained at work. The lab I worked in had over 100 Abs. No way did I have the time at work to commit to the initial document. It does take time but is well worth the effort. Rena Fail ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R [arsenn@hsh.org] Sent: Friday, February 22, 2008 10:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi all... This may be a silly question & I could probably find the answer myself by searching the Internet or our own archives-but I'm allowed only so much time on the computer at work & for the next 2 weeks, I'm on another 'position' so I won't be near the computer at all...So please accept my apologies... We are looking for a 'cheat sheet' for special and immuno stains. We need the name of the stain, the best control tissue, what we're staining (looking) for, and the colors we will see if the stain is done properly. I've searched for this online, but I find SOOO much information packed into tables that it will take me hours to delete what I don't need & enter the information I DO need. I just thought if someone had something like this handy, I could have a copy....if not, I'll just go to the library this weekend and begin making a chart myself from the sticky notes we have all over the lab LOL Also, thank you all who responded to my Calret. question! I received a lot of suggestions, and we're trying them all! Have a great Friday everyone! Amy Histotech, Histology Laboratory Holy Spirit Hospital 503 N. 21st Street Camp Hill, PA 17011 (717) 763-2124 Cell: (724) 494-2237 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Cytch7 <@t> cox.net Fri Feb 22 10:40:33 2008 From: Cytch7 <@t> cox.net (Valerie Biendara) Date: Fri Feb 22 10:40:39 2008 Subject: [Histonet] Job Opening, Cytotechnologist Message-ID: <000001c87571$a598d4f0$f80a010a@nwapath.com> NWA Pathology Assoc, Fayetteville, Arkansas, has an opening for a full-time Cytotechnologist. We do 47,000 Gyn, most of which are ThinPrepR, and 1,500 Non-gyn. We utilize the Cytyc Imager. We would like some one who has done some Molecular Pathology or is interested in learning it. The laboratory building is brand new and the cytology office is spacious. Please contact: Valerie Biendara SCT(ASCP)IAC Cytology Supervisor NWA Pathology Assoc. Cytch7@cox.net 479-442-0144 X 124 479-442-4557 (Fax) From ccpath <@t> gmail.com Fri Feb 22 10:47:28 2008 From: ccpath <@t> gmail.com (j k) Date: Fri Feb 22 10:47:37 2008 Subject: [Histonet] Job Opening, Cytotechnologist In-Reply-To: <000001c87571$a598d4f0$f80a010a@nwapath.com> References: <000001c87571$a598d4f0$f80a010a@nwapath.com> Message-ID: Looking for a nonagency (preferably) cytotech for a one week locum in April. Beautiful coastal carolina. jim keller, md ccpath@gmail.com From pkromund <@t> gundluth.org Fri Feb 22 11:33:34 2008 From: pkromund <@t> gundluth.org (pkromund@gundluth.org) Date: Fri Feb 22 11:33:44 2008 Subject: Fw: [Histonet] Dako Info Message-ID: Hello All, I contacted my DAKO rep to inquire about the recent DAKO histonet questions. Here is his response below: ----- Forwarded by Pamela K Romundstad/CytHist/LAX/GUNDLUTH on 02/22/2008 11:31 AM ----- "Bill Domansky" To 02/21/2008 03:48 cc PM Subject RE: [Histonet] Dako Info Pam, What you have read on Histonet is completely untrue. Dako is NOT planning any discontinuation on any of the EnVision detection. This is a rumor. If I could ask that you post this information onto Histonet and also note that if anyone is concerned about this they should contact their Dako Account Manager as you have. I would appreciate your help. Thanks and I'll see you next week. -----Original Message----- From: pkromund@gundluth.org Sent: Thursday, February 21, 2008 3:26 PM To: Bill.Domansky@dako.com Subject: Fw: [Histonet] Dako Info Hi Bill, Do you know anything about this? Pamela Romundstad Gundersen Lutheran 1910 South Ave No. LaCrosse, WI 54601 608-775-3139 ----- Forwarded by Pamela K Romundstad/CytHist/LAX/GUNDLUTH on 02/21/2008 01:57 PM ----- "Nita Searcy" To Sent by: histonet-bounces@ cc lists.utsouthwest ern.edu Subject [Histonet] Dako Info 02/20/2008 11:13 AM Posting for Lead Immunohistochemistry Technologist: Anyone using Dako Envision & Detection system heard about it being discontinued? What substitutions are you considering? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> Stowers-Institute.org Fri Feb 22 16:32:08 2008 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Fri Feb 22 16:32:28 2008 Subject: [Histonet] Formalin (from Paraformaldehyde) stability Message-ID: I had a question today from a researcher asking about the stability of a formalin solution made from Paraformaldehyde. She indicated that on one of her samples, freshly prepared fixative worked well, while one day old fixative (same batch) did not fix properly. She assures me all pre-fixative steps were the same. The samples were fixed for 2 hours. I have no details about her experiments or what she was testing (I suspect mRNA targets). I realize that without the addition of methanol, the solution is not as stable would would re-polymerize with time. I'm wondering if the fixative started re-polymerizing and that made the difference, but one day? Is it possible for one day to make the difference between a positive and negative result? How stable/instable is methylene glycol? I'm also thinking that by adding methanol (or using purchased 100% formalin (37% formaldehyde)) would be useful for mRNA targets, since aldehydes tend to fix nucleic acids poorly. Why do all my most difficult questions arise on Friday afternoon? Thanks for any insight you can give me. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From rydomsal <@t> yahoo.com Sat Feb 23 03:59:39 2008 From: rydomsal <@t> yahoo.com (Ryan Dominique Salazar) Date: Sat Feb 23 03:59:43 2008 Subject: [Histonet] saturated picric acid Message-ID: <545365.88376.qm@web52304.mail.re2.yahoo.com> Sorry I forgot to tell that the raw picric acid I'm telling is damped inside the bottle. Thanks, for the info. Whew, fire in the hole! --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From gu.lang <@t> gmx.at Sat Feb 23 05:17:54 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Feb 23 05:18:02 2008 Subject: AW: [Histonet] Formalin (from Paraformaldehyde) stability In-Reply-To: Message-ID: <000001c8760d$bd09fd20$eeeea8c0@dielangs.at> I think, the kind of her test would be of interest. If she dissolves the mRNA out of the tissue a fixation shouldn't be "too good". As a result a weak fixative works better for this test. On the other side, when she does in situ hybridization on the tissue it has to be a "good" fixation. In John Kiernans book stands regarding Fomal-saline, that is has to be made a day before use to let depolymerization to take place. Then it can be kept for several months. Regarding NBF made with paraformaldehyde it says, that it can be kept also for several months. My assumption is, that the older fixative makes its job too good for her purposes and that could result in mRNA degradation. And in a publication I read was mentioned, that with methods, where the AAA-tail of the RNA is used to isolate it, the adhesion of methylol-groups on the Adenin (via formaldehyd) can disturb it. Maybe my suggestion can help. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Johnson, Teri Gesendet: Freitag, 22. Februar 2008 23:32 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Formalin (from Paraformaldehyde) stability I had a question today from a researcher asking about the stability of a formalin solution made from Paraformaldehyde. She indicated that on one of her samples, freshly prepared fixative worked well, while one day old fixative (same batch) did not fix properly. She assures me all pre-fixative steps were the same. The samples were fixed for 2 hours. I have no details about her experiments or what she was testing (I suspect mRNA targets). I realize that without the addition of methanol, the solution is not as stable would would re-polymerize with time. I'm wondering if the fixative started re-polymerizing and that made the difference, but one day? Is it possible for one day to make the difference between a positive and negative result? How stable/instable is methylene glycol? I'm also thinking that by adding methanol (or using purchased 100% formalin (37% formaldehyde)) would be useful for mRNA targets, since aldehydes tend to fix nucleic acids poorly. Why do all my most difficult questions arise on Friday afternoon? Thanks for any insight you can give me. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sat Feb 23 11:25:53 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Feb 23 11:25:57 2008 Subject: [Histonet] Formalin (from Paraformaldehyde) stability In-Reply-To: Message-ID: <98189.18110.qm@web61221.mail.yahoo.com> Once the paraformaldehyde solution is prepared, it should be kep in refrigeration (at about 5?C). Ren? J. "Johnson, Teri" wrote: I had a question today from a researcher asking about the stability of a formalin solution made from Paraformaldehyde. She indicated that on one of her samples, freshly prepared fixative worked well, while one day old fixative (same batch) did not fix properly. She assures me all pre-fixative steps were the same. The samples were fixed for 2 hours. I have no details about her experiments or what she was testing (I suspect mRNA targets). I realize that without the addition of methanol, the solution is not as stable would would re-polymerize with time. I'm wondering if the fixative started re-polymerizing and that made the difference, but one day? Is it possible for one day to make the difference between a positive and negative result? How stable/instable is methylene glycol? I'm also thinking that by adding methanol (or using purchased 100% formalin (37% formaldehyde)) would be useful for mRNA targets, since aldehydes tend to fix nucleic acids poorly. Why do all my most difficult questions arise on Friday afternoon? Thanks for any insight you can give me. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From WWmn916 <@t> aol.com Sun Feb 24 20:09:49 2008 From: WWmn916 <@t> aol.com (WWmn916@aol.com) Date: Sun Feb 24 20:10:06 2008 Subject: [Histonet] Ammonia water and H+E stain Message-ID: I recently asked this on the histonet, and would like to get some more info on it. Could soaking faced paraffin blocks in ammonia water too long negatively affect the H+E stain? I Could ammonia change the acid/base charges or pH of different tissue components that could negatively effect the charges necessary for heme staining, or eosin? Thanks for letting me "rerun" this question **************Ideas to please picky eaters. Watch video on AOL Living. (http://living.aol.com/video/how-to-please-your-picky-eater/rachel-campos-duffy/ 2050827?NCID=aolcmp00300000002598) From Kemlo.Rogerson <@t> waht.swest.nhs.uk Mon Feb 25 02:10:06 2008 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Feb 25 02:10:10 2008 Subject: [Histonet] Ammonia water and H+E stain Message-ID: <86ADE4EB583CE64799A9924684A0FBBF03CAE19B@wahtntex2.waht.swest.nhs.uk> "I recently asked this on the histonet, and would like to get some more info on it. Could soaking faced paraffin blocks in ammonia water too long negatively affect the H+E stain? I Could ammonia change the acid/base charges or pH of different tissue components that could negatively effect the charges necessary for heme staining, or eosin? Thanks for letting me "rerun" this question" If you leave something in anything too long then something is likely to alter, but why do it? I suppose that opens the wound again of you Americans poorly fixing and processing stuff so that you resort to cutting frozen sections at room temperature!!! Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Don't be afraid to take a big step when one is indicated. You can't cross a chasm in two small jumps. --Buckminster Fuller This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From marktarango <@t> gmail.com Mon Feb 25 03:59:05 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Mon Feb 25 03:59:11 2008 Subject: [Histonet] Ammonia water and H+E stain In-Reply-To: References: Message-ID: <5b6eb13e0802250159r4eacf9d2m4076ae563277fc17@mail.gmail.com> Yes, both get screwed up if they're in ammonia too long. You might notice the eosin looking weak before anything else. Can you adjust the processing times so they don't come out so dry (then you might not need to use ammonia water so often). I remember when I worked for Impath, they made a rule that you had to ask a lead tech before you could use any ammonia water. At some point someone will forget a block there and ruin the specimen. I don't think it's always appropriate to use ammonia water either. For something that is overprocessed/dry or bloody, its a great trick. You shouldn't have to use it too often though. Mark T. On Sun, Feb 24, 2008 at 6:09 PM, wrote: > I recently asked this on the histonet, and would like to get some more > info > on it. > Could soaking faced paraffin blocks in ammonia water too long negatively > affect the H+E stain? I > > Could ammonia change the acid/base charges or pH of different tissue > components that could negatively effect the charges necessary for heme > staining, or > eosin? > > Thanks for letting me "rerun" this question > > > > > > **************Ideas to please picky eaters. Watch video on AOL Living. > ( > http://living.aol.com/video/how-to-please-your-picky-eater/rachel-campos-duffy/ > 2050827?NCID=aolcmp00300000002598) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From PattiReiser <@t> pmchealthsystem.org Mon Feb 25 06:08:17 2008 From: PattiReiser <@t> pmchealthsystem.org (PattiReiser@pmchealthsystem.org) Date: Mon Feb 25 06:08:38 2008 Subject: [Histonet] MPO-PAS Stain Message-ID: Does anyone have a procedure for a used on bone marrow smears? Pat Pocono Medical Cent East Stroudsburg, PA From mpence <@t> grhs.net Mon Feb 25 08:10:15 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Feb 25 08:10:29 2008 Subject: [Histonet] New pan-cytokeratin monoclonal antibody Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A372F@IS-E2K3.grhs.net> Hi All, Has anyone heard of or using the new OSCAR pan-cytokeratin monoclonal antibody from Signet Labs. I hear it does not have the same problems as the conventional pan-keratin stains. If you are using it, what system are you using it on? Thanks, Mike From ploykasek <@t> phenopath.com Mon Feb 25 11:00:12 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Mon Feb 25 11:00:24 2008 Subject: [Histonet] New pan-cytokeratin monoclonal antibody In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A372F@IS-E2K3.grhs.net> Message-ID: HI Mike. Our lab director, Dr. Allen Gown is the developer of OSCAR. What problems have you had with the other pan-keratins? I'll try to answer your questions. Of course, I think OSCAR is a very nice pan-keratin. It is what we use for our pan-keratin. Our unannounced CAP inspection just started. So, I'm off to that. I love Mondays. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Hi All, > Has anyone heard of or using the new OSCAR pan-cytokeratin monoclonal > antibody from Signet Labs. I hear it does not have the same problems as > the conventional pan-keratin stains. If you are using it, what system > are you using it on? > > Thanks, > Mike > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From slappycraw <@t> yahoo.com Mon Feb 25 11:06:54 2008 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Mon Feb 25 11:07:00 2008 Subject: [Histonet] Mast cell Tryptase and Chymase Message-ID: <487335.18678.qm@web53603.mail.re2.yahoo.com> I would like to know if anyone out there in research land has had any success detecting mast cells with tryptase or chymase antibodies on mouse tissue? Thanks in advance. Larry A. Woody Seattle, Wa. --------------------------------- Never miss a thing. Make Yahoo your homepage. From dmccaig <@t> ckha.on.ca Mon Feb 25 13:53:14 2008 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Mon Feb 25 13:53:24 2008 Subject: [Histonet] RED COUNTERSTAINS Message-ID: In the past we have had great red counterstains when using Nuclear Fast Red and Neutral Red. Recently it does not matter how fresh, concentration or times of these two stain, they appear pale and washed out. Any ideas what could be causing this or how it could be remedied. diana From settembr <@t> umdnj.edu Mon Feb 25 13:58:36 2008 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Mon Feb 25 13:59:18 2008 Subject: [Histonet] Ret Message-ID: Looking for any information on someone who may have Ret working on FFPE human tissue. I understand it is tough to get to work. Does anyone have any info? Vendor? Dilution? Thank you, Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA From akemiat3377 <@t> yahoo.com Mon Feb 25 15:28:23 2008 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Mon Feb 25 15:28:32 2008 Subject: [Histonet] Unannounced CAP Inspection Message-ID: <250173.91186.qm@web31314.mail.mud.yahoo.com> Oh Joy, It's Monday and we are having our CAP inspection! Great way to start the week! Akemi Allison-Tacha, BS, HT(ASCP)HTL Client Services Manager PhenoPath laboratories 551 North 34th Street, Suite 100 Seattle, WA 98103-8675 Work: (206) 374-9000 Cell: (425) 941-4287 E-Mail: akemiat3377@yahoo.com From LSebree <@t> uwhealth.org Mon Feb 25 15:32:17 2008 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Mon Feb 25 15:32:25 2008 Subject: [Histonet] Unannounced CAP Inspection In-Reply-To: <250173.91186.qm@web31314.mail.mud.yahoo.com> Message-ID: We made it through a few weeks ago; you will too! Have courage. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Akemi Allison-Tacha Sent: Monday, February 25, 2008 3:28 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Unannounced CAP Inspection Oh Joy, It's Monday and we are having our CAP inspection! Great way to start the week! Akemi Allison-Tacha, BS, HT(ASCP)HTL Client Services Manager PhenoPath laboratories 551 North 34th Street, Suite 100 Seattle, WA 98103-8675 Work: (206) 374-9000 Cell: (425) 941-4287 E-Mail: akemiat3377@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Mon Feb 25 15:40:48 2008 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Feb 25 15:40:56 2008 Subject: [Histonet] hard tissue committee Message-ID: I have a request from one of my graduates for help from someone from the hard tissue committee. She has a questions regarding DNA on a temporal bone biopsy. Can anybody help out? I can forward your information to her so that she can ask specific questions. Thank you, Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu From p_bourne_14526 <@t> yahoo.com Mon Feb 25 15:59:59 2008 From: p_bourne_14526 <@t> yahoo.com (Patricia Bourne) Date: Mon Feb 25 16:00:04 2008 Subject: SPAM-LOW: RE: [Histonet] CAP guidelines Message-ID: <190184.16329.qm@web51707.mail.re2.yahoo.com> OK..here a a question for the group. In the lab where I worked we had included the patient's name and surgical number, block number etc. Then one day a sales rep came in and stated that having the patients name of the slide was a HIPPA violation. So the director immediately stopped using the patient name. The patient name was used for QA monitoring (making sure the right case was ordered etc.) and was used for sorting at sign-out. Never saw this in writing and have asked several other Pathologist about this issue and they all laughed and said "maybe we should take the name off the report". So I ask this group....has anyone ever heard of this in there travels? Thanks ----- Original Message ---- From: Hector Hernandez To: "Horn, Hazel V" ; Douglas D Deltour ; Sharon.Davis-Devine ; histonet@lists.utsouthwestern.edu Sent: Thursday, February 21, 2008 2:17:37 PM Subject: RE: SPAM-LOW: RE: [Histonet] CAP guidelines Let me put it another way. As Douglas stated on CAP question ANP.21100 Phase II N/A YES NO Are blocks identified adequately? NOTE: Each block of tissue must be identified by the entire accession number assigned to the case and by any descriptive letter(s)/number(s) added by the prosector during the dissection. If additional blocks are prepared later, all lists and logs must reflect these additions. Identification number and letter(s)/numbers(s) must be affixed to all blocks in a manner that remains legible. It doesn't state anything about two identifiers. -----Original Message----- From: Horn, Hazel V [mailto:HornHV@archildrens.org] Sent: Thursday, February 21, 2008 3:31 PM To: Douglas D Deltour; Hector Hernandez; Sharon.Davis-Devine; histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: RE: [Histonet] CAP guidelines I disagree as well. I don't see it in the guidelines published September 07. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Thursday, February 21, 2008 3:21 PM To: 'Hector Hernandez'; 'Sharon.Davis-Devine'; histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: RE: [Histonet] CAP guidelines I disagree with that interpretation Hector. I do not consider the block number a patient identifier. Still I consider Sharon's original question hearsay until I see it on a CAP checklist. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hector Hernandez Sent: Thursday, February 21, 2008 4:01 PM To: 'Douglas D Deltour'; 'Sharon.Davis-Devine'; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: RE: [Histonet] CAP guidelines I think everyone in the Histo world already does this. Example S1000-07 A1, A2, etc. No need to get excited about this question. The two identifiers are the surgical# and any sub letter or number. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Thursday, February 21, 2008 2:48 PM To: 'Sharon.Davis-Devine'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CAP guidelines This is from the Sep 2007 checklist. ANP.21100 Phase II N/A YES NO Are blocks identified adequately? NOTE: Each block of tissue must be identified by the entire accession number assigned to the case and by any descriptive letter(s)/number(s) added by the prosector during the dissection. If additional blocks are prepared later, all lists and logs must reflect these additions. Identification number and letter(s)/numbers(s) must be affixed to all blocks in a manner that remains legible. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Thursday, February 21, 2008 3:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP guidelines Ok, Histonetters, got another burning question for you. I have heard that there is a new CAP guideline that states that there needs to be two patient identifiers on the tissue block. We have a block printer and we all know that there is very limited space on the block for much info, so if we need to have two patient identifiers things will get very tight. For those of you who have block printers, how are you handling this? All info and suggestions are greatly appreciated. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ From lhunt <@t> uta.edu Mon Feb 25 16:13:43 2008 From: lhunt <@t> uta.edu (Laura Hunt) Date: Mon Feb 25 16:13:52 2008 Subject: [Histonet] filtering H&E In-Reply-To: References: Message-ID: <9A5D3C83-4D19-4EDB-83AD-A459846C2D24@uta.edu> What are some suggested means to filter H&E prior to use. I am going to try the SurgiPath reagents but I am also making H&E from scratch. Do I just pour the stains thru a whatman filter lined funnel? Thanks! LH From victor <@t> pathology.washington.edu Mon Feb 25 16:59:27 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Mon Feb 25 16:59:43 2008 Subject: SPAM-LOW: RE: [Histonet] CAP guidelines In-Reply-To: <190184.16329.qm@web51707.mail.re2.yahoo.com> References: <190184.16329.qm@web51707.mail.re2.yahoo.com> Message-ID: <47C3484F.5050802@pathology.washington.edu> It is not a HIPAA violation in the normal course of your day to day workflow. It is a violation only if the slides got into unauthorized hands. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Patricia Bourne wrote: > OK..here a a question for the group. In the lab where I worked we had included the patient's name and surgical number, block number etc. Then one day a sales rep came in and stated that having the patients name of the slide was a HIPPA violation. So the director immediately stopped using the patient name. The patient name was used for QA monitoring (making sure the right case was ordered etc.) and was used for sorting at sign-out. Never saw this in writing and have asked several other Pathologist about this issue and they all laughed and said "maybe we should take the name off the report". So I ask this group....has anyone ever heard of this in there travels? > > Thanks > > > ----- Original Message ---- > From: Hector Hernandez > To: "Horn, Hazel V" ; Douglas D Deltour ; Sharon.Davis-Devine ; histonet@lists.utsouthwestern.edu > Sent: Thursday, February 21, 2008 2:17:37 PM > Subject: RE: SPAM-LOW: RE: [Histonet] CAP guidelines > > Let me put it another way. As Douglas stated on CAP question ANP.21100 > Phase II N/A YES NO > > Are blocks identified adequately? > > NOTE: Each block of tissue must be identified by the entire accession > number assigned to the case and by any descriptive letter(s)/number(s) > added > by the prosector during the dissection. If additional blocks are > prepared > later, all lists and logs must reflect these additions. Identification > number and letter(s)/numbers(s) must be affixed to all blocks in a > manner > that remains legible. > > It doesn't state anything about two identifiers. > > -----Original Message----- > From: Horn, Hazel V [mailto:HornHV@archildrens.org] > Sent: Thursday, February 21, 2008 3:31 PM > To: Douglas D Deltour; Hector Hernandez; Sharon.Davis-Devine; > histonet@lists.utsouthwestern.edu > Subject: RE: SPAM-LOW: RE: [Histonet] CAP guidelines > > I disagree as well. I don't see it in the guidelines published > September 07. > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Histology > Arkansas Children's Hospital > 800 Marshall Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax 501.364.3912 > > visit us on the web at: www.archildrens.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas > D Deltour > Sent: Thursday, February 21, 2008 3:21 PM > To: 'Hector Hernandez'; 'Sharon.Davis-Devine'; > histonet@lists.utsouthwestern.edu > Subject: RE: SPAM-LOW: RE: [Histonet] CAP guidelines > > I disagree with that interpretation Hector. I do not consider the block > number a patient identifier. Still I consider Sharon's original question > hearsay until I see it on a CAP checklist. > > Douglas D. Deltour HT(ASCP) > Histology Manager > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > Office (803)252-1913 > Fax (803)254-3262 > Doug@ppspath.com > ***************************************************** > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of the individual or entity to > which it is addressed and may contain information that is privileged, > confidential and exempt from disclosure under applicable law. If the > reader > of this message is not the intended recipient, you are hereby notified > that > any dissemination, distribution, or copying of this communication is > strictly prohibited by law. If you have received this communication in > error, please notify me immediately. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hector > Hernandez > Sent: Thursday, February 21, 2008 4:01 PM > To: 'Douglas D Deltour'; 'Sharon.Davis-Devine'; > histonet@lists.utsouthwestern.edu > Subject: SPAM-LOW: RE: [Histonet] CAP guidelines > > I think everyone in the Histo world already does this. Example S1000-07 > A1, > A2, etc. No need to get excited about this question. The two > identifiers > are the surgical# and any sub letter or number. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas > D > Deltour > Sent: Thursday, February 21, 2008 2:48 PM > To: 'Sharon.Davis-Devine'; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] CAP guidelines > > This is from the Sep 2007 checklist. > > ANP.21100 Phase II N/A YES NO > > Are blocks identified adequately? > > NOTE: Each block of tissue must be identified by the entire accession > number assigned to the case and by any descriptive letter(s)/number(s) > added > by the prosector during the dissection. If additional blocks are > prepared > later, all lists and logs must reflect these additions. Identification > number and letter(s)/numbers(s) must be affixed to all blocks in a > manner > that remains legible. > > Douglas D. Deltour HT(ASCP) > Histology Manager > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > Office (803)252-1913 > Fax (803)254-3262 > Doug@ppspath.com > ***************************************************** > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of the individual or entity to > which it is addressed and may contain information that is privileged, > confidential and exempt from disclosure under applicable law. If the > reader > of this message is not the intended recipient, you are hereby notified > that > any dissemination, distribution, or copying of this communication is > strictly prohibited by law. If you have received this communication in > error, please notify me immediately. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Sharon.Davis-Devine > Sent: Thursday, February 21, 2008 3:15 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] CAP guidelines > > Ok, Histonetters, got another burning question for you. I have heard > that there is a new CAP guideline that states that there needs to be two > patient identifiers on the tissue block. We have a block printer and we > all know that there is very limited space on the block for much info, so > if we need to have two patient identifiers things will get very tight. > For those of you who have block printers, how are you handling this? > All info and suggestions are greatly appreciated. > > > > Sharon Davis-Devine, CT (ASCP) > > Cytology Supervisor > > Carle Clinic > > 602 West University > > Urbana, Illinois 61801 > > Phone: 217-383-3572 > > Email: sharon.davis-devine@carle.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ____________________________________________________________________________________ > Be a better friend, newshound, and > know-it-all with Yahoo! Mobile. Try it now. http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JMacDonald <@t> mtsac.edu Mon Feb 25 17:48:50 2008 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Feb 25 18:57:44 2008 Subject: [Histonet] (no subject) In-Reply-To: Message-ID: There is an article in Sakura's Histologic that address IHC controls. Also check IHC manufacturer's catalogs or websites. Some have great charts. http://www.sakura-americas.com/histologic/pdf/98_may.pdf Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu "Senn, Amy R" Sent by: histonet-bounces@lists.utsouthwestern.edu 02/22/2008 07:13 AM To cc Subject [Histonet] (no subject) Hi all... This may be a silly question & I could probably find the answer myself by searching the Internet or our own archives-but I'm allowed only so much time on the computer at work & for the next 2 weeks, I'm on another 'position' so I won't be near the computer at all...So please accept my apologies... We are looking for a 'cheat sheet' for special and immuno stains. We need the name of the stain, the best control tissue, what we're staining (looking) for, and the colors we will see if the stain is done properly. I've searched for this online, but I find SOOO much information packed into tables that it will take me hours to delete what I don't need & enter the information I DO need. I just thought if someone had something like this handy, I could have a copy....if not, I'll just go to the library this weekend and begin making a chart myself from the sticky notes we have all over the lab LOL Also, thank you all who responded to my Calret. question! I received a lot of suggestions, and we're trying them all! Have a great Friday everyone! Amy Histotech, Histology Laboratory Holy Spirit Hospital 503 N. 21st Street Camp Hill, PA 17011 (717) 763-2124 Cell: (724) 494-2237 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Mon Feb 25 16:50:43 2008 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Feb 25 18:58:05 2008 Subject: [Histonet] microtome repair Message-ID: I would like to thank everyone for the responses that I received regarding the repair of our Shandon Finesse 325. A service rep from Thermo-Fisher came out and repaired the microtome. It was a minor adjustment needed. While the service rep was here she was kind enough to inspect our other two of that model to make sure that they are operating properly. A special Thank you to the staff at Thermo-Fisher. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu From marktarango <@t> gmail.com Tue Feb 26 04:40:44 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Tue Feb 26 04:40:52 2008 Subject: [Histonet] MPO-PAS Stain In-Reply-To: References: Message-ID: <5b6eb13e0802260240x2ccd07d3te1ba44232f7d1e6a@mail.gmail.com> I think Sigma's kit has a protocol in their product insert that I remember reading. On Mon, Feb 25, 2008 at 4:08 AM, wrote: > > Does anyone have a procedure for a combination MPO-PAS stain to be > used on bone marrow smears? Thanks. > > > Pat ti Reiser MTHT, QIHC (ASCP) > > Pocono Medical Cent er > > East Stroudsburg, PA > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From marktarango <@t> gmail.com Tue Feb 26 05:16:25 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Tue Feb 26 05:16:37 2008 Subject: [Histonet] RED COUNTERSTAINS In-Reply-To: References: Message-ID: <5b6eb13e0802260316gfaa4e57l2c2342d59c095474@mail.gmail.com> I like using Richard-Allen's 7211 hematoxylin for counterstains like in the iron stain. I do a minute w/ hematoxylin, rinse, and then a minute in their clarifier II. If you use distilled water the hematoxylin stays red and looks great. If you have any lying around, it can't hurt to try. On Mon, Feb 25, 2008 at 11:53 AM, Diana McCaig wrote: > In the past we have had great red counterstains when using Nuclear Fast > Red and Neutral Red. > > Recently it does not matter how fresh, concentration or times of these > two stain, they appear > pale and washed out. > > Any ideas what could be causing this or how it could be remedied. > > diana > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tanisha.mcknight <@t> covance.com Tue Feb 26 07:52:58 2008 From: tanisha.mcknight <@t> covance.com (McKnight, Tanisha) Date: Tue Feb 26 07:53:08 2008 Subject: [Histonet] HELP! Overheated Water Bath Message-ID: <816E3C72F855F14985FC31D7C963AE6F04BFED42@indexch03.ent.covance.com> Hello All: Quick question. The overheat indicator on my water bath keeps coming on and won't heat up even though it has been disconnected from a power source for some time. Is there a way to reset this mechanism or should I call someone to come look at it. Tanisha N. McKnight ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From Janet.Bonner <@t> FLHOSP.ORG Tue Feb 26 07:56:57 2008 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Tue Feb 26 08:00:18 2008 Subject: [Histonet] HELP! Overheated Water Bath References: <816E3C72F855F14985FC31D7C963AE6F04BFED42@indexch03.ent.covance.com> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F254D@fhosxchmb006.ADVENTISTCORP.NET> This sounds like an electrical problem as well as a motherboard problem. What do you mean "disconnected from a power source"....unplugged? ...and it's still got an indicator coming on? Toss it - it's possessed!!! ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of McKnight, Tanisha Sent: Tue 2/26/2008 8:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HELP! Overheated Water Bath Hello All: Quick question. The overheat indicator on my water bath keeps coming on and won't heat up even though it has been disconnected from a power source for some time. Is there a way to reset this mechanism or should I call someone to come look at it. Tanisha N. McKnight ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From rjbuesa <@t> yahoo.com Tue Feb 26 08:06:05 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 26 08:06:13 2008 Subject: [Histonet] HELP! Overheated Water Bath In-Reply-To: <816E3C72F855F14985FC31D7C963AE6F04BFED42@indexch03.ent.covance.com> Message-ID: <546468.89867.qm@web61225.mail.yahoo.com> I am confused: 1- is the indicator comming up but the water does not heat?, or 2- is the indicator comming up and the water heats up? In any event those water heaters are very simple, there is just a thermostat and some connections, there is nothing to "reset". Call somebody to look at it. Ren? J. "McKnight, Tanisha" wrote: Hello All: Quick question. The overheat indicator on my water bath keeps coming on and won't heat up even though it has been disconnected from a power source for some time. Is there a way to reset this mechanism or should I call someone to come look at it. Tanisha N. McKnight ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From beverly <@t> histologytechservices.com Tue Feb 26 10:00:55 2008 From: beverly <@t> histologytechservices.com (Beverly Robinson) Date: Tue Feb 26 09:53:42 2008 Subject: [Histonet] Job opportunity - Gainesville, FL Message-ID: <0MKpCa-1JU27c2nad-0007LD@mrelay.perfora.net> Senior Histotechnician or Histotechnologist - with HT or HTL (ASCP), Florida licensed. Must have excellent microtomy skills and a good working knowledge of IHC. Histology Tech Services is a small "Histology Only" GLP and CLIA certified lab where you will work and be cross trained on all phases of Biotech, Donor, Human and Animal Diagnostic and Research preparations of tissue. Applicant must be mature and have good organization skills with attention to detail. We are production oriented but take the time to produce excellent quality slides for our clients. Full time Days, 5 days a week, no weekends. Will consider part time. Competitive wages and Benefits. Please Fax Resume to Beverly @ 352-338-0027 or email to: Beverly@histologytechservices.com Beverly Robinson HT (ASCP) Laboratory Manager Histology Tech Services, Inc Gainesville, FL Phone: 352-338-0045 FAX: 352-338-0027 This email and its attachments (if any) contain confidential and privileged information. Any dissemination or use of this information by a person other than the intended recipient is unauthorized and may be illegal. From integrated.histo <@t> gmail.com Tue Feb 26 10:12:24 2008 From: integrated.histo <@t> gmail.com (Cindy DuBois) Date: Tue Feb 26 10:12:33 2008 Subject: [Histonet] Night differential Message-ID: <5d9104a30802260812m401c9869ge50ebec893545fbe@mail.gmail.com> Are there any techs who get a night differential? If so, how much? We currently come to work @ 3:30 am. We are now being asked to come in at 1:30. I would like to ask for a night differential, but need to know how much to ask for. Thanks, Cindy From rjbuesa <@t> yahoo.com Tue Feb 26 11:06:08 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 26 11:06:14 2008 Subject: [Histonet] Night differential In-Reply-To: <5d9104a30802260812m401c9869ge50ebec893545fbe@mail.gmail.com> Message-ID: <544555.65566.qm@web61218.mail.yahoo.com> The only way you do not get a night differential is if you do not work more than 40h/week including the night shift, and even so, many labs pay a differential that goes from 8PM to 6AM and that is usually from $0.25 to $0.50/hour Ren? J. Cindy DuBois wrote: Are there any techs who get a night differential? If so, how much? We currently come to work @ 3:30 am. We are now being asked to come in at 1:30. I would like to ask for a night differential, but need to know how much to ask for. Thanks, Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. From doug <@t> ppspath.com Tue Feb 26 11:38:23 2008 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Feb 26 11:39:27 2008 Subject: SPAM-LOW: Re: [Histonet] Night differential In-Reply-To: <544555.65566.qm@web61218.mail.yahoo.com> Message-ID: Labs that are asking their techs to work these hours and that are not paying a differential are not staying competitive. It is hard enough to find techs that will work but to find ones that willing to work these hours is very difficult. A differential is not too much to ask. IMO .25 to .50 is too low. That would not motivate me to want to change my hours to 0130. The majority of our work is completed during those hours also and we pay $1 hr for dif. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, February 26, 2008 12:06 PM To: Cindy DuBois; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: Re: [Histonet] Night differential The only way you do not get a night differential is if you do not work more than 40h/week including the night shift, and even so, many labs pay a differential that goes from 8PM to 6AM and that is usually from $0.25 to $0.50/hour Ren? J. Cindy DuBois wrote: Are there any techs who get a night differential? If so, how much? We currently come to work @ 3:30 am. We are now being asked to come in at 1:30. I would like to ask for a night differential, but need to know how much to ask for. Thanks, Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Tue Feb 26 11:56:21 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Feb 26 11:53:39 2008 Subject: [Histonet] Night differential In-Reply-To: References: <544555.65566.qm@web61218.mail.yahoo.com> Message-ID: <1F937FB30BDB7C4A9F39F83FEA8D379F9F5273@bruexchange1.digestivespecialists.com> To put shift differential in perspective, 25 years ago I was getting .50 and hour shift differential. I think that is should have gone up by now. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, February 26, 2008 12:38 PM To: 'Rene J Buesa'; 'Cindy DuBois'; histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: Re: [Histonet] Night differential Labs that are asking their techs to work these hours and that are not paying a differential are not staying competitive. It is hard enough to find techs that will work but to find ones that willing to work these hours is very difficult. A differential is not too much to ask. IMO .25 to .50 is too low. That would not motivate me to want to change my hours to 0130. The majority of our work is completed during those hours also and we pay $1 hr for dif. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, February 26, 2008 12:06 PM To: Cindy DuBois; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: Re: [Histonet] Night differential The only way you do not get a night differential is if you do not work more than 40h/week including the night shift, and even so, many labs pay a differential that goes from 8PM to 6AM and that is usually from $0.25 to $0.50/hour Ren? J. Cindy DuBois wrote: Are there any techs who get a night differential? If so, how much? We currently come to work @ 3:30 am. We are now being asked to come in at 1:30. I would like to ask for a night differential, but need to know how much to ask for. Thanks, Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Crystalmo <@t> fmchealth.org Tue Feb 26 11:57:42 2008 From: Crystalmo <@t> fmchealth.org (Crystal Morris) Date: Tue Feb 26 11:57:50 2008 Subject: [Histonet] Items for Sale Message-ID: I have a few items for sale if anyone is interested please email me and I can get you all the details. Here is the list: Dako Automated Immunostainer w/ some unopened antibodies (not expired yet), purchased new in 2004 Biocare Medical Decloaking Chamber, age unknown Fisher Scientific Isotemp 102, age unknown Thanks Crystal Morris M.T.(ASCP) Anatomical Supervisor Fairfield Medical Center Laboratory 401 N. Ewing St. Lancaster, Ohio 43130 (740) 687-8807 "Confidentiality Notice: This e-mail message, including any attachments is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review; use; disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message." From DStillings <@t> unipathllc.com Tue Feb 26 11:57:55 2008 From: DStillings <@t> unipathllc.com (Donella Stillings) Date: Tue Feb 26 11:58:07 2008 Subject: SPAM-LOW: Re: [Histonet] Night differential In-Reply-To: References: <544555.65566.qm@web61218.mail.yahoo.com> Message-ID: <8785CEF5DCC41A4F8014179C435935D602D276@exchange.unipathllc.corp> In our lab we get $4.00/hr but 1/2 of your shift must be worked before 7:30am -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, February 26, 2008 10:38 AM To: 'Rene J Buesa'; 'Cindy DuBois'; histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: Re: [Histonet] Night differential Labs that are asking their techs to work these hours and that are not paying a differential are not staying competitive. It is hard enough to find techs that will work but to find ones that willing to work these hours is very difficult. A differential is not too much to ask. IMO .25 to .50 is too low. That would not motivate me to want to change my hours to 0130. The majority of our work is completed during those hours also and we pay $1 hr for dif. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, February 26, 2008 12:06 PM To: Cindy DuBois; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: Re: [Histonet] Night differential The only way you do not get a night differential is if you do not work more than 40h/week including the night shift, and even so, many labs pay a differential that goes from 8PM to 6AM and that is usually from $0.25 to $0.50/hour Ren? J. Cindy DuBois wrote: Are there any techs who get a night differential? If so, how much? We currently come to work @ 3:30 am. We are now being asked to come in at 1:30. I would like to ask for a night differential, but need to know how much to ask for. Thanks, Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Tue Feb 26 12:04:45 2008 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Feb 26 12:06:13 2008 Subject: SPAM-LOW: Re: [Histonet] Night differential In-Reply-To: <1F937FB30BDB7C4A9F39F83FEA8D379F9F5273@bruexchange1.digestivespecialists.com> Message-ID: That was probably the price of gas per gallon back then too. :) Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Tuesday, February 26, 2008 12:56 PM To: Cindy DuBois; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: Re: [Histonet] Night differential To put shift differential in perspective, 25 years ago I was getting .50 and hour shift differential. I think that is should have gone up by now. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, February 26, 2008 12:38 PM To: 'Rene J Buesa'; 'Cindy DuBois'; histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: Re: [Histonet] Night differential Labs that are asking their techs to work these hours and that are not paying a differential are not staying competitive. It is hard enough to find techs that will work but to find ones that willing to work these hours is very difficult. A differential is not too much to ask. IMO .25 to .50 is too low. That would not motivate me to want to change my hours to 0130. The majority of our work is completed during those hours also and we pay $1 hr for dif. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, February 26, 2008 12:06 PM To: Cindy DuBois; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: Re: [Histonet] Night differential The only way you do not get a night differential is if you do not work more than 40h/week including the night shift, and even so, many labs pay a differential that goes from 8PM to 6AM and that is usually from $0.25 to $0.50/hour Ren? J. Cindy DuBois wrote: Are there any techs who get a night differential? If so, how much? We currently come to work @ 3:30 am. We are now being asked to come in at 1:30. I would like to ask for a night differential, but need to know how much to ask for. Thanks, Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug <@t> ppspath.com Tue Feb 26 12:14:33 2008 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Feb 26 12:15:30 2008 Subject: SPAM-LOW: Re: [Histonet] Night differential In-Reply-To: <8785CEF5DCC41A4F8014179C435935D602D276@exchange.unipathllc.corp> Message-ID: That is a pretty generous shift dif. Well done. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Donella Stillings Sent: Tuesday, February 26, 2008 12:58 PM To: Douglas D Deltour; Rene J Buesa; Cindy DuBois; histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: Re: [Histonet] Night differential In our lab we get $4.00/hr but 1/2 of your shift must be worked before 7:30am -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D Deltour Sent: Tuesday, February 26, 2008 10:38 AM To: 'Rene J Buesa'; 'Cindy DuBois'; histonet@lists.utsouthwestern.edu Subject: RE: SPAM-LOW: Re: [Histonet] Night differential Labs that are asking their techs to work these hours and that are not paying a differential are not staying competitive. It is hard enough to find techs that will work but to find ones that willing to work these hours is very difficult. A differential is not too much to ask. IMO .25 to .50 is too low. That would not motivate me to want to change my hours to 0130. The majority of our work is completed during those hours also and we pay $1 hr for dif. Douglas D. Deltour HT(ASCP) Histology Manager Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 Office (803)252-1913 Fax (803)254-3262 Doug@ppspath.com ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, February 26, 2008 12:06 PM To: Cindy DuBois; histonet@lists.utsouthwestern.edu Subject: SPAM-LOW: Re: [Histonet] Night differential The only way you do not get a night differential is if you do not work more than 40h/week including the night shift, and even so, many labs pay a differential that goes from 8PM to 6AM and that is usually from $0.25 to $0.50/hour Ren? J. Cindy DuBois wrote: Are there any techs who get a night differential? If so, how much? We currently come to work @ 3:30 am. We are now being asked to come in at 1:30. I would like to ask for a night differential, but need to know how much to ask for. Thanks, Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Looking for last minute shopping deals? Find them fast with Yahoo! Search. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Tue Feb 26 12:22:41 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Feb 26 12:20:10 2008 Subject: [Histonet] Items for Sale In-Reply-To: References: Message-ID: <1F937FB30BDB7C4A9F39F83FEA8D379F9F5274@bruexchange1.digestivespecialists.com> Speaking of items for sale! I have 2 Microm DS/50 slide stainers that are less than two years old for sale. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Crystal Morris Sent: Tuesday, February 26, 2008 12:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Items for Sale I have a few items for sale if anyone is interested please email me and I can get you all the details. Here is the list: Dako Automated Immunostainer w/ some unopened antibodies (not expired yet), purchased new in 2004 Biocare Medical Decloaking Chamber, age unknown Fisher Scientific Isotemp 102, age unknown Thanks Crystal Morris M.T.(ASCP) Anatomical Supervisor Fairfield Medical Center Laboratory 401 N. Ewing St. Lancaster, Ohio 43130 (740) 687-8807 "Confidentiality Notice: This e-mail message, including any attachments is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review; use; disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message." From bob.nienhuis <@t> gmail.com Tue Feb 26 12:32:18 2008 From: bob.nienhuis <@t> gmail.com (Bob Nienhuis) Date: Tue Feb 26 12:32:27 2008 Subject: [Histonet] Osmium and Golgi Message-ID: <45109da50802261032n388ba2fdqde30463df9d3abd7@mail.gmail.com> Anyone know if the use of osmium tetroxide is necessary in rapid Golgi staining? I know that it is nasty stuff, and would like to avoid using it if possible Doing rapid Golgi staining in formail-fixed tissue, and will be observing with light micropscope. Bob Nienhuis UCLA / VA Medical Center Neurobiology Research I From ROrr <@t> enh.org Tue Feb 26 12:37:04 2008 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Tue Feb 26 12:37:08 2008 Subject: [Histonet] any workshops for HT exam? Message-ID: HI everyone Does anyone know if any of the state meetings in the Midwest or Michigan will be having a workshop that helps prep for the HT/HTL exam? Thanks Beckt Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 From RSRICHMOND <@t> aol.com Tue Feb 26 13:25:38 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Tue Feb 26 13:25:45 2008 Subject: [Histonet] Re: RED COUNTERSTAINS Message-ID: Diana McCaig asks about the nuclear counterstains nuclear fast red and neutral red. Does anyone on this list have any experience with Anatech's "Brazilliant", their trade name for alum brazilin, closely related to alum hematoxylin, but red instead of purple? This looks to me like a very logical red nuclear stain, and I'd certainly like to see it in action if it were possible for me to obtain it (remember that hospital pathology services are not usually permitted to order from small companies like Anatech and the Davidson marking ink people). As everybody on this list ought to know, hematoxylin is a dye extracted from the logwood tree (Haematoxylum campechianum), with an aluminum mordant. (There is no satisfactory synthetic substitute.) Brazilin is structurally very similar, but with an alum mordant it is red rather than purple. Brazilin is extracted from the closely related brazil woods, Caesalpinia echinata or C. sappan. One would expect this red dye to have the same staining specificity as hematoxylin, and it should not wash out in aqueous mounting media. (I have no connection with Anatech.) Bob Richmond Samurai Pathologist Knoxville TN From ebreisch <@t> rchsd.org Tue Feb 26 13:27:23 2008 From: ebreisch <@t> rchsd.org (Breisch, Eric) Date: Tue Feb 26 13:27:33 2008 Subject: [Histonet] pathologist's assistant opening in San Diego Message-ID: <43B97B4C402C2C44AAA2A8D2C86A88B31A5A83@e2k3backend1.RCHSD.org> Hi Histonetters, This is just a heads up that Rady Children's Hospital and Health Center has an opening for a Pathologist's Assistant position in the Department of Pathology. The position is full time with full benefits. Hours are flexible but generally from 0830 to 1700 hours. Experience in histology is a decided advantage. We have a moderate case load at approximately 13-14,000 specimens per year and approximately 30 necropsies per year. We have four pathologists that are extremely easy to work with. Southern California has a rather pleasant climate and San Diego is conveniently located near many recreational opportunities. For interested candidates please send a CV to the address listed below or contact me at ebreisch@rchsd.org (858-966-5944) and I will be happy to provide more detailed information. Thank you. Eric Send to : Rady Children's Hospital and Health Center Department of Pathology/Division of Histology MC 5007 3020 Children's Way San Diego, CA 92123 Eric A. Breisch, Ph.D. Clinical Anatomist Dept. of Pathology Rady Children's Hospital and Health Center Associate Clinical Professor of Anatomy Dept. of Surgery UCSD School of Medicine From akbitting <@t> geisinger.edu Tue Feb 26 14:35:34 2008 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Tue Feb 26 14:35:57 2008 Subject: [Histonet] qc testing detection Message-ID: <47C431C6.2B7F.00C9.0@geisinger.edu> Hello Histonetters, What methods are people using to validate new lots of antibody and detection before they are put into use? Do you incorporate them into your normal work flow, or do you do QC runs? What batteries of tissue do you use? Just curious what others are doing. Thanks all! Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From asachau <@t> titanmed.com Tue Feb 26 14:44:06 2008 From: asachau <@t> titanmed.com (April Sachau) Date: Tue Feb 26 14:47:02 2008 Subject: [Histonet] Histology position In-Reply-To: <47C431C6.2B7F.00C9.0@geisinger.edu> Message-ID: <7E3ACD48BA6E26408F3188FBF08693F701188429@titansbs1.corp.titanmed.com> Hello Histonetters! I am looking for an ASCP certified HT or HTL for a traveling, or temporary assignment I have available (mid March start date - approximately 13 + week assignment). This position is located in the Midwest, pay package as follows: - Fully furnished housing provided or $1200.00 tax-free monthly housing allowance - Car allowance ($150.00 weekly) or Rental Car provided - Flight to and from assignment, or mileage paid (.365 cents to and from assignment) provided - $210.00 tax-free weekly per diem provided Anyone seriously interested, please reply. Thanks! April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com From rjbuesa <@t> yahoo.com Tue Feb 26 14:50:49 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Feb 26 14:50:58 2008 Subject: [Histonet] qc testing detection In-Reply-To: <47C431C6.2B7F.00C9.0@geisinger.edu> Message-ID: <400.22154.qm@web61223.mail.yahoo.com> At least try the new lot with the usual (+) control with the usual protocol and if everything turns as expected, document that test. IF it does not react as expected, then you will have to "start all over again" with the required checker board tests, etc. Ren? J. Angela Bitting wrote: Hello Histonetters, What methods are people using to validate new lots of antibody and detection before they are put into use? Do you incorporate them into your normal work flow, or do you do QC runs? What batteries of tissue do you use? Just curious what others are doing. Thanks all! Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From asachau <@t> titanmed.com Tue Feb 26 14:50:49 2008 From: asachau <@t> titanmed.com (April Sachau) Date: Tue Feb 26 14:53:43 2008 Subject: [Histonet] Histology position In-Reply-To: <7E3ACD48BA6E26408F3188FBF08693F701188429@titansbs1.corp.titanmed.com> Message-ID: <7E3ACD48BA6E26408F3188FBF08693F701188440@titansbs1.corp.titanmed.com> Hourly pay rate for position is $24.00/hour for the dayshift and $25.00 for night shift. April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of April Sachau Sent: Tuesday, February 26, 2008 2:44 PM To: Angela Bitting; histonet Subject: [Histonet] Histology position Hello Histonetters! I am looking for an ASCP certified HT or HTL for a traveling, or temporary assignment I have available (mid March start date - approximately 13 + week assignment). This position is located in the Midwest, pay package as follows: - Fully furnished housing provided or $1200.00 tax-free monthly housing allowance - Car allowance ($150.00 weekly) or Rental Car provided - Flight to and from assignment, or mileage paid (.365 cents to and from assignment) provided - $210.00 tax-free weekly per diem provided Anyone seriously interested, please reply. Thanks! April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From oshel1pe <@t> cmich.edu Tue Feb 26 14:59:01 2008 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Tue Feb 26 14:59:15 2008 Subject: [Histonet] book & web site recommendations Message-ID: Listers, I got a few answers from my request for class text recommendations. Several people also mentioned the plethora of websites with basic light microscopy information useful for introductory classes, so I've included a few of my bookmarks. It was nice to find the AFIP Methods manual still available, although Kiernan's text isn't. (Arg!) Foster's book "Optimizing the Light Microscope ..." is also out of print, although she still has a few copies left (there may be a new edition sometime, possibly). A major criterion was expense. There are lots of books available for $80 to $100 and more, a few of which cover both the basics of light microscopy and microscopes *and* microtechnique, but there are very few of these in the light microscopy realm. Unlike the EM world. Phil Books: Douglas Murphy; Fundamentals of Light Microscopy and Electronic Imaging put out by Wiley. Basic Methods in Microscopy: Protocols And Concepts from Cells, a Laboratory Manual (Paperback) David L. Spector (Editor), Robert D. Goldman (Editor) Introduction to Light Microscopy (Microscopy Handbooks) (Paperback) by Mrs H Bradbury Royal Microscopy Society Contrast Techniques in Light Microscopy, by Bradbury & Bracegirdle 1996 Royal Microscopy Society Plus, several of the other books in the RMS series Microscope series by Mortimer Abramowitz http://www.olympusamerica.com/seg_section/seg_emporiumbooks.asp "Laboratory Methods in Histotechnology" E.B. Prophet et al. AFIP LABORATORY METH <=also known as FS07 $35.00 + $5.00 shp Prepayment is required on all orders. Fax your prepaid order to 1-301-578-1693. If you would rather pay by check please mail your order to: AMERICAN REGISTRY OF PATHOLOGY PUBLICATIONS DEPT. PO BOX 8188 SILVER SPRING, MD 20907 We accept VISA, Master Card and American Express. publications@arppress.org Web sites: Nikon Microscopy U http://www.microscopyu.com/sitemap.html Olympus Microscopy Resource http://www.olympusmicro.com/ Molecular Expressions http://micro.magnet.fsu.edu/index.html (Molecular Expressions) Molecular Probes Fluorescence http://probes.invitrogen.com/resources/education/ -- Philip Oshel Microscopy Facility Supervisor Biology Department 024C Brooks Hall Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576 From rgrow <@t> bmnet.com Tue Feb 26 15:01:47 2008 From: rgrow <@t> bmnet.com (rgrow@bmnet.com) Date: Tue Feb 26 15:01:53 2008 Subject: [Histonet] Renee Grow is out of the office. Message-ID: I will be out of the office starting 02/26/2008 and will not return until 03/07/2008. I will respond to your message when I return. ________________________________ This communication may contain protected health information (PHI) that is legally protected from inappropriate disclosure by the Privacy Standards of the Health Insurance Portability and Accountability Act (HIPAA) and relevant Tennessee Laws. If you are not the intended recipient, please note that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this message in error, you should notify the sender immediately by telephone or by return e-mail and delete this message from your computer. Direct questions to the Blount Memorial Hospital Privacy Officer at 865-977-4675. From Heather.D.Renko <@t> osfhealthcare.org Tue Feb 26 15:14:22 2008 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Tue Feb 26 15:15:09 2008 Subject: [Histonet] Re; two patient identifiers Message-ID: <40026EDDE64CDA47AB382C52619ACD3C0A09DD89@pmc-rfd-mx01.intranet.osfnet.org> This is a Joint Commission Goal: 2008 National Patient Safety Goals Laboratory Services Program Goal 1 Improve the accuracy of patient identification. 1A Use at least two patient identifiers when providing care, treatment or services http://www.jointcommission.org/PatientSafety/NationalPatientSafetyGoals/08_lab_npsgs.htm Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From asmith <@t> mail.barry.edu Tue Feb 26 15:17:40 2008 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Tue Feb 26 15:17:54 2008 Subject: [Histonet] Re: RED COUNTERSTAINS In-Reply-To: Message-ID: I have not used Anatech's Brazilliant, but I have made alum brazillin ("brazalum") from brazillin and ammonium alum. Because the stain is not as dark as hematoxylin, I like it for thick sections. It is colorfast in organic solvents and holds up well in Permount: slides that I stained with brazalum and naphthol green 40 years ago are still as good as the day I made them. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert Richmond Sent: Tuesday, February 26, 2008 2:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: RED COUNTERSTAINS Diana McCaig asks about the nuclear counterstains nuclear fast red and neutral red. Does anyone on this list have any experience with Anatech's "Brazilliant", their trade name for alum brazilin, closely related to alum hematoxylin, but red instead of purple? This looks to me like a very logical red nuclear stain, and I'd certainly like to see it in action if it were possible for me to obtain it (remember that hospital pathology services are not usually permitted to order from small companies like Anatech and the Davidson marking ink people). As everybody on this list ought to know, hematoxylin is a dye extracted from the logwood tree (Haematoxylum campechianum), with an aluminum mordant. (There is no satisfactory synthetic substitute.) Brazilin is structurally very similar, but with an alum mordant it is red rather than purple. Brazilin is extracted from the closely related brazil woods, Caesalpinia echinata or C. sappan. One would expect this red dye to have the same staining specificity as hematoxylin, and it should not wash out in aqueous mounting media. (I have no connection with Anatech.) Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Tue Feb 26 15:30:14 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Feb 26 15:30:19 2008 Subject: [Histonet] Histology position In-Reply-To: <7E3ACD48BA6E26408F3188FBF08693F701188429@titansbs1.corp.titanmed.com> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A3739@IS-E2K3.grhs.net> Federal milage is now $.505 per mile. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of April Sachau Sent: Tuesday, February 26, 2008 2:44 PM To: Angela Bitting; histonet Subject: [Histonet] Histology position Hello Histonetters! I am looking for an ASCP certified HT or HTL for a traveling, or temporary assignment I have available (mid March start date - approximately 13 + week assignment). This position is located in the Midwest, pay package as follows: - Fully furnished housing provided or $1200.00 tax-free monthly housing allowance - Car allowance ($150.00 weekly) or Rental Car provided - Flight to and from assignment, or mileage paid (.365 cents to and from assignment) provided - $210.00 tax-free weekly per diem provided Anyone seriously interested, please reply. Thanks! April Sachau Titan Medical Group Staff Supervisor Phone (866) 332-9600 Ext. 1023 Fax (402) 332-5181 asachau@titanmed.com see us on the web at www.titanmed.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> Mercer.edu Tue Feb 26 15:47:55 2008 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Tue Feb 26 15:52:10 2008 Subject: [Histonet] Region III Hosted by Georgia Society for Histotechnology Message-ID: <01MRRC2REH9Y001BO3@Macon2.Mercer.edu> Hi Guys, We sent out over 1000 programs for the Region III meeting to be held in Atlanta Georgia April 3-5, 2008 at the Westin Peachtree Plaza Hotel. But there may be some members that we missed and feel it necessary to post a reminder for all who wish to attend our meeting. The deadline for the hotel reservations for the discounted rate is March 3rd. That rate is $129 single, double, triple or quad. The phone number to the Westin is 1-404-659-1400 and please state that you are attending the GSH/Region III meeting. The revised program can be downloaded from our website at www.histosearch.com/gsh. Click on the symposium link to get a PDF file of the program. Vendors have a link to the registration form to exhibit at the meeting on the same page. If you have questions Chris Coley, GSH Exhibit Liaison, has contact information is on that form If anyone has any questions please feel to contact me at this email address. Come on down to Georgia and experience some warmer weather. Shirley Powell GSH Secretary/Registrar From nicholasprosenbaum <@t> yahoo.com Tue Feb 26 16:25:46 2008 From: nicholasprosenbaum <@t> yahoo.com (Nicholas Rosenbaum) Date: Tue Feb 26 16:25:55 2008 Subject: [Histonet] Night differential Message-ID: <834073.71274.qm@web32608.mail.mud.yahoo.com> We get $3.50/hour for shift differential. If you come in before 4:30am you get the shift differential for your entire shift. If you come in at 4:30am you get the differential for half of your shift. Any later than 4:30 and you just get your hourly wage. Nicholas P. Rosenbaum HT, ASCP University of Virginia Medical Center Charlottesville, VA ____________________________________________________________________________________ Never miss a thing. Make Yahoo your home page. http://www.yahoo.com/r/hs From ohenry <@t> dfw.net Tue Feb 26 16:31:01 2008 From: ohenry <@t> dfw.net (Susan Owens) Date: Tue Feb 26 16:29:10 2008 Subject: [Histonet] Re: Night differential Message-ID: <009e01c878c7$44c20490$ebf763d8@your4f1261a8e5> Cindy...... At our hospital, Histo currently gets the same night differential as the main clinical lab (not always the case.). The work day is divided into three (3) pay shifts (1st, 2nd and 3rd)...The hours you ask about are third shift and get the highest shift dollars...I currently start work at 3:00am and get a $ 2.50 per hour shift differential But only for the hours worked in the third shift..For us, the 1st shift starts at 6:00am so my shift diff is paid to me for the hours 3:00am to 6:00am(or 3 hours ea day). If I come in early (or late) then my 3rd shift hours are increased or decreased accordingly.. Susan Date: Tue, 26 Feb 2008 08:12:24 -0800 From: "Cindy DuBois" Subject: [Histonet] Night differential Are there any techs who get a night differential? If so, how much? We currently come to work @ 3:30 am. We are now being asked to come in at 1:30. I would like to ask for a night differential, but need to know how much to ask for. Thanks, Cindy From Pathrm35 <@t> comcast.net Tue Feb 26 18:06:51 2008 From: Pathrm35 <@t> comcast.net (Pathrm35@comcast.net) Date: Tue Feb 26 18:06:56 2008 Subject: SPAM-LOW: Re: [Histonet] Night differential Message-ID: <022720080006.28493.47C4A99B0003CD8A00006F4D2215575474CACC039D089B0EAF@comcast.net> We get a 25% diff for working the overnight shift. (8:30pm-5am) -------------- Original message -------------- From: "Douglas D Deltour" > That is a pretty generous shift dif. Well done. > > Douglas D. Deltour HT(ASCP) > Histology Manager > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > Office (803)252-1913 > Fax (803)254-3262 > Doug@ppspath.com > ***************************************************** > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of the individual or entity to > which it is addressed and may contain information that is privileged, > confidential and exempt from disclosure under applicable law. If the reader > of this message is not the intended recipient, you are hereby notified that > any dissemination, distribution, or copying of this communication is > strictly prohibited by law. If you have received this communication in > error, please notify me immediately. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Donella > Stillings > Sent: Tuesday, February 26, 2008 12:58 PM > To: Douglas D Deltour; Rene J Buesa; Cindy DuBois; > histonet@lists.utsouthwestern.edu > Subject: RE: SPAM-LOW: Re: [Histonet] Night differential > > In our lab we get $4.00/hr but 1/2 of your shift must be worked before > 7:30am > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas D > Deltour > Sent: Tuesday, February 26, 2008 10:38 AM > To: 'Rene J Buesa'; 'Cindy DuBois'; histonet@lists.utsouthwestern.edu > Subject: RE: SPAM-LOW: Re: [Histonet] Night differential > > Labs that are asking their techs to work these hours and that are not paying > a differential are not staying competitive. It is hard enough to find techs > that will work but to find ones that willing to work these hours is very > difficult. A differential is not too much to ask. IMO .25 to .50 is too low. > That would not motivate me to want to change my hours to 0130. The majority > of our work is completed during those hours also and we pay $1 hr for dif. > > Douglas D. Deltour HT(ASCP) > Histology Manager > Professional Pathology Services, PC > One Science Court > Suite 200 > Columbia, SC 29203 > Office (803)252-1913 > Fax (803)254-3262 > Doug@ppspath.com > ***************************************************** > PROFESSIONAL PATHOLOGY SERVICES, PC > NOTICE OF CONFIDENTIALITY > This message is intended only for the use of the individual or entity to > which it is addressed and may contain information that is privileged, > confidential and exempt from disclosure under applicable law. If the reader > of this message is not the intended recipient, you are hereby notified that > any dissemination, distribution, or copying of this communication is > strictly prohibited by law. If you have received this communication in > error, please notify me immediately. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Tuesday, February 26, 2008 12:06 PM > To: Cindy DuBois; histonet@lists.utsouthwestern.edu > Subject: SPAM-LOW: Re: [Histonet] Night differential > > The only way you do not get a night differential is if you do not work more > than 40h/week including the night shift, and even so, many labs pay a > differential that goes from 8PM to 6AM and that is usually from $0.25 to > $0.50/hour > René J. > > Cindy DuBois wrote: > Are there any techs who get a night differential? If so, how much? > We currently come to work @ 3:30 am. We are now being asked to come in at > 1:30. I would like to ask for a night differential, but need to know how > much to ask for. > > Thanks, > Cindy > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Looking for last minute shopping deals? Find them fast with Yahoo! Search. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thisisann <@t> aol.com Tue Feb 26 18:39:20 2008 From: thisisann <@t> aol.com (thisisann@aol.com) Date: Tue Feb 26 18:39:30 2008 Subject: [Histonet] Histology Supervisor Position/Bryn Mawr, Pa Message-ID: <8CA46F0533B0910-1A80-C0E@mblk-d41.sysops.aol.com> List, There is currently a Histology Supervisor position open at a prestigious Urology Group located in Bryn Mawr, Pa.? This person should possess the skills to set-up, maintain and successful build upon a state-of-the-art Histology laboratory.? Knowledge of technics required to gross, process and microtome small fine needle biopsies as well as IHC/Ventana experience required. Qualified applicants, please fax your resume to:? Histology Manager, 908-272-1478. ________________________________________________________________________ More new features than ever. Check out the new AOL Mail ! - http://webmail.aol.com From WWmn916 <@t> aol.com Tue Feb 26 22:23:17 2008 From: WWmn916 <@t> aol.com (WWmn916@aol.com) Date: Tue Feb 26 22:23:36 2008 Subject: [Histonet] All about water Message-ID: Greetings histo friends, Has anyone jumped through the new CAP water hoops? CAP indicates Clinical and Laboratory Standards Institute (instead of NCCLS guidelines for Type I, II, III water) The CLSI guidelines "Prepartion and Testing of Reagent Water in the Clinical Laboratory" gives a few sample pages on line, but I'm left wondering if Type II water will translate easily under CLSI. My current procedure references our lab water as Type II and NCCLS guidelines. It's looking like I need to rewrite a few things. Is this correct? Are labs required to have on hand the CLSI guideline ($120.00 !!!) Is NCCLS outdated and no longer required to have on hand? Thanks everyone Deb King, HT(ASCP) Sacramento, CA **************Ideas to please picky eaters. Watch video on AOL Living. (http://living.aol.com/video/how-to-please-your-picky-eater/rachel-campos-duffy/ 2050827?NCID=aolcmp00300000002598) From lcheah <@t> agric.wa.gov.au Wed Feb 27 02:01:43 2008 From: lcheah <@t> agric.wa.gov.au (Cheah, Lit Chien) Date: Wed Feb 27 02:09:39 2008 Subject: [Histonet] Fixing crab blood smear Message-ID: Dear All, I have been ask is there a batter way to fix crab blood smear before the slides travel 800 kilometers for us to do Giemsa Stain, appart from the normal air dry then fix in methanol and shipment. Big Thanks in advance from the largest State in Australia! Mr. Lit Chien CHEAH Histopathology Supervisor BSC (Medical Science) Animal Health Laboratories 3 Baron-Hay Court South Perth, 6155 WA. This e-mail and files transmitted with it are privileged and confidential information intended for the use of the addressee. The confidentiality and/or privilege in this e-mail is not waived, lost or destroyed if it has been transmitted to you in error. If you received this e-mail in error you must (a) not disseminate, copy or take any action in reliance on it; (b) please notify the Department of Agriculture and Food, WA immediately by return e-mail to the sender; (c) please delete the original e-mail. This email has been successfully scanned by McAfee Anti-Virus software. Department of Agriculture and Food WA From benoit.delatour <@t> u-psud.fr Wed Feb 27 04:10:09 2008 From: benoit.delatour <@t> u-psud.fr (=?ISO-8859-1?Q?Beno=EEt_Delatour?=) Date: Wed Feb 27 03:58:38 2008 Subject: [Histonet] Myelin stain with Gold chloride Message-ID: <47C53701.5070204@u-psud.fr> Dear histoneters, We are currently trying to use the gold chloride (GC) method (Schmued, Journal of Histochemistry and Cytochemistry 38, 717, May, 1990) to provide quantitative assessment of myelination / demyelination in mouse brain tissue. From the literature it appears that optical densities of GC or myelin basic protein immunostained material both allow good evaluation of myelin contents in fiber tracks. Our concern is the following : we have setup two GC protocols, method 1 that gives an overall low but significant staining (good labeling of major fiber pathways but radiating myelinated fibers in the cortex are only weakly stained), and method 2 that provides very strong myelin staining including fibers in the isocortex and the hippocampus. We are wondering which protocol is the most suitable for quantitative analysis of myelin. In our experiments, we found with method 1 differences in the myelination of corpus callosum between our experimental groups. However using method 2, it seems that these differences are strongly attenuated - we think this might be due to some background overstaining or saturation effect that could obliterate subtle differences in myelination between animals. I thank you very much in advance for all your answers and comments, B. Delatour -- Laboratoire de Neurobiologie de l'Apprentissage, de la M?moire & de la Communication, NAMC, CNRS UMR 8620, B?t 446 Universit? Paris-Sud 91405 Orsay Cedex, FRANCE Tel 33 (0)1 69 15 49 88 Fax 33 (0)1 69 15 77 26 Email benoit.delatour@u-psud.fr Web http://www.namc.u-psud.fr If you think research is expensive, try disease. From kmerriam2003 <@t> yahoo.com Wed Feb 27 06:42:25 2008 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Wed Feb 27 06:42:28 2008 Subject: [Histonet] FISH cytology fixatives Message-ID: <668890.34731.qm@web50312.mail.re2.yahoo.com> Hi Everyone, I am working up some FISH staining protocols for cytology (cell culture) slides (eventually to be OCT and FFPE tissues, but I need to walk before I can run). Most of the journals that I have read recommend using a fixative containing methanol and acetic acid (3:1). The DAKO probe I am using calls for the use of 3.7% fomaldehyde. I am wondering why acetone (or acetone/ethanol) is not the routine fixative for such a procedure. Can someone let me know why to choose one fixative over another and what the merits of methanol/glacial acetic acid would be? Thanks in advance, Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA --------------------------------- Never miss a thing. Make Yahoo your homepage. From godsgalnow <@t> aol.com Wed Feb 27 07:24:11 2008 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Wed Feb 27 07:24:36 2008 Subject: [Histonet] FISH cytology fixatives In-Reply-To: <668890.34731.qm@web50312.mail.re2.yahoo.com> References: <668890.34731.qm@web50312.mail.re2.yahoo.com> Message-ID: <8CA475B2C9387AD-804-4E7@FWM-M08.sysops.aol.com> In Cytological preparation, one would use an acetic acid in the fixative to lyse the RBC's, which could ultimaetly interfere with the diagnosis.? Journal articles are great to help you research and give you a starting point for what it is you want to do, that is where I started.? But when you start doing the procedure, or playing with until you get it to work, you always need to start with the suggestions of the manufacturer of the probe.? That is how they validated it and it is known to work under those conditions.? At that point you can play with fixatives, if you need to. A lot of FISH testing requires post fixation anyway.? For, instance, the tissue comes in formalin (or whatever), but after you de-wax the slides you have to begin the pre-treatment, an most of the time there is a step in there that involves placing the slides in formalin (or whatever). Roxanne -----Original Message----- From: Kim Merriam To: Histonet Sent: Wed, 27 Feb 2008 7:42 am Subject: [Histonet] FISH cytology fixatives Hi Everyone, I am working up some FISH staining protocols for cytology (cell culture) slides (eventually to be OCT and FFPE tissues, but I need to walk before I can run). Most of the journals that I have read recommend using a fixative containing methanol and acetic acid (3:1). The DAKO probe I am using calls for the use of 3.7% fomaldehyde. I am wondering why acetone (or acetone/ethanol) is not the routine fixative for such a procedure. Can someone let me know why to choose one fixative over another and what the merits of methanol/glacial acetic acid would be? Thanks in advance, Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA --------------------------------- Never miss a thing. Make Yahoo your homepage. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Feb 27 07:37:27 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Feb 27 07:37:35 2008 Subject: [Histonet] Fixing crab blood smear In-Reply-To: Message-ID: <927292.99610.qm@web61223.mail.yahoo.com> Your procedure is standard, even for crab or spiny lobster for that matter (even when it really is hemolymph). They should be OK Do you know Dr. Phillips (working with spiny lobsters? I have lost contact with him since many years ago). Ren? J. Buesa "Cheah, Lit Chien" wrote: Dear All, I have been ask is there a batter way to fix crab blood smear before the slides travel 800 kilometers for us to do Giemsa Stain, appart from the normal air dry then fix in methanol and shipment. Big Thanks in advance from the largest State in Australia! Mr. Lit Chien CHEAH Histopathology Supervisor BSC (Medical Science) Animal Health Laboratories 3 Baron-Hay Court South Perth, 6155 WA. This e-mail and files transmitted with it are privileged and confidential information intended for the use of the addressee. The confidentiality and/or privilege in this e-mail is not waived, lost or destroyed if it has been transmitted to you in error. If you received this e-mail in error you must (a) not disseminate, copy or take any action in reliance on it; (b) please notify the Department of Agriculture and Food, WA immediately by return e-mail to the sender; (c) please delete the original e-mail. This email has been successfully scanned by McAfee Anti-Virus software. Department of Agriculture and Food WA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From cmiller <@t> physlab.com Wed Feb 27 08:22:25 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Wed Feb 27 08:21:10 2008 Subject: [Histonet] Night differential In-Reply-To: <5d9104a30802260812m401c9869ge50ebec893545fbe@mail.gmail.com> References: <5d9104a30802260812m401c9869ge50ebec893545fbe@mail.gmail.com> Message-ID: <001501c8794c$2cd6f720$3d02a8c0@plab.local> Where I used to work it was 15% of your hourly wage for any hours worked from 1100pm to 6am Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy DuBois Sent: Tuesday, February 26, 2008 10:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Night differential Are there any techs who get a night differential? If so, how much? We currently come to work @ 3:30 am. We are now being asked to come in at 1:30. I would like to ask for a night differential, but need to know how much to ask for. Thanks, Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From Simoskevitz <@t> Osteotech.com Wed Feb 27 09:19:52 2008 From: Simoskevitz <@t> Osteotech.com (Simoskevitz@Osteotech.com) Date: Wed Feb 27 09:23:02 2008 Subject: [Histonet] goldners trichrome Message-ID: Barbara, I was asked to do a Goldners Trichrome in Methymethacylate embedded bone . Do you have a good protocol for this or do you know where I could find one. Thank you for any help you can give me. Ricki Simoskevitz Osteotech Inc. Eatontown, NJ 07724 (732) 542-2800 X6328 From histosprv06 <@t> aol.com Wed Feb 27 09:29:35 2008 From: histosprv06 <@t> aol.com (histosprv06@aol.com) Date: Wed Feb 27 09:29:51 2008 Subject: [Histonet] Lyme Disease IHC/PCR Message-ID: <8CA476CB1044DEB-80C-AE3@webmail-da16.sysops.aol.com> Good Morning everyone, no better place to come for advice than the histonet! Does anyone know of a facility where?we can send a processed skin block for Borrelia Burgdorferi via IHC and PCR as well as Erlichiosis via PCR? We are in Florida and are not having much luck finding?a place?that offers this testing.? We would greatly appreciate any knowledge you can provide. Thanks in advance. ? Kari Zajic, HT From rmweber113 <@t> comcast.net Wed Feb 27 10:36:07 2008 From: rmweber113 <@t> comcast.net (rmweber113@comcast.net) Date: Wed Feb 27 10:38:00 2008 Subject: [Histonet] Histology Supervisor Position NY Message-ID: <022720081636.3418.47C591770005560100000D5A2215575114CCCECE9D0A0D0A99039D@comcast.net> I have a position for a Histology Supervisor available at an established GI group located in New City, NY, Rockland County. Candidate should have the knowledge to set up and maintain a state of the art facility. Knowledge of grossing, processing and routine histology of gastric biopsies required. $30.00/hr plus benefits. Qualified candidates can email me at rmweber113@comcast.net or call me at 732 814-1170 $500.00 for a successful referals committed for at least 90 days. Marilynn Weber H.T. (ASCP) QIHC Twincrest From GAshton <@t> picr.man.ac.uk Wed Feb 27 10:39:18 2008 From: GAshton <@t> picr.man.ac.uk (Garry Ashton) Date: Wed Feb 27 10:39:29 2008 Subject: [Histonet] reference point slides. Message-ID: Hi everyone, Has anybody used or had any experience of microscope slides with some type of reference point on them? Thanks in advance. Garry PICR UK -------------------------------------------------------- This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the University of Manchester. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From GAshton <@t> picr.man.ac.uk Wed Feb 27 10:39:18 2008 From: GAshton <@t> picr.man.ac.uk (Garry Ashton) Date: Wed Feb 27 10:39:33 2008 Subject: [Histonet] reference point slides. Message-ID: Hi everyone, Has anybody used or had any experience of microscope slides with some type of reference point on them? Thanks in advance. Garry PICR UK -------------------------------------------------------- This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the University of Manchester. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From Erin.Martin <@t> ucsf.edu Wed Feb 27 11:06:53 2008 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Wed Feb 27 11:07:06 2008 Subject: [Histonet] Dull eosin? Message-ID: Hi everyone, I am having a problem with my eosin in the H&E. My pathologists say that it is dull and the RBCs are too red rather than orange. Interestingly, when I take out the hematoxylin (Richard Allen Harris), acid acohol (Richard Allen Clarifier) and bluing (Richard Allen Bluing) the eosin is nice and bright. Our tap water for the rinses is pH 6.8, and we use a Sakura Prisma stainer. Any ideas? Thank in advance! Erin Martin From mpence <@t> grhs.net Wed Feb 27 11:13:35 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Feb 27 11:13:45 2008 Subject: [Histonet] Dull eosin? In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A373E@IS-E2K3.grhs.net> What eosin are you using? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Erin Sent: Wednesday, February 27, 2008 11:07 AM To: histonet Subject: [Histonet] Dull eosin? Hi everyone, I am having a problem with my eosin in the H&E. My pathologists say that it is dull and the RBCs are too red rather than orange. Interestingly, when I take out the hematoxylin (Richard Allen Harris), acid acohol (Richard Allen Clarifier) and bluing (Richard Allen Bluing) the eosin is nice and bright. Our tap water for the rinses is pH 6.8, and we use a Sakura Prisma stainer. Any ideas? Thank in advance! Erin Martin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> CDC.GOV Wed Feb 27 11:22:28 2008 From: jqb7 <@t> CDC.GOV (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Wed Feb 27 11:23:42 2008 Subject: [Histonet] Dull eosin? In-Reply-To: References: Message-ID: <34BB307EFC9A65429BBB49E330675F72045E25FA@LTA3VS003.ees.hhs.gov> I assume you are using the Richard Allan eosin Y alcoholic? Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Erin Sent: Wednesday, February 27, 2008 12:07 PM To: histonet Subject: [Histonet] Dull eosin? Hi everyone, I am having a problem with my eosin in the H&E. My pathologists say that it is dull and the RBCs are too red rather than orange. Interestingly, when I take out the hematoxylin (Richard Allen Harris), acid acohol (Richard Allen Clarifier) and bluing (Richard Allen Bluing) the eosin is nice and bright. Our tap water for the rinses is pH 6.8, and we use a Sakura Prisma stainer. Any ideas? Thank in advance! Erin Martin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> Mercer.edu Wed Feb 27 11:25:09 2008 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Wed Feb 27 11:29:31 2008 Subject: [Histonet] FW: Region III Hosted by Georgia Society for Histotechnology Message-ID: <01MRSH6CO2WQ001E0L@Macon2.Mercer.edu> I sent this out yesterday, but I never received it myself so this is just to make sure you guys get it. -----Original Message----- From: Shirley Powell [mailto:powell_sa@mercer.edu] Sent: Tuesday, February 26, 2008 4:48 PM To: 'histonet@lists.utsouthwestern.edu' Subject: Region III Hosted by Georgia Society for Histotechnology Hi Guys, We sent out over 1000 programs for the Region III meeting to be held in Atlanta Georgia April 3-5, 2008 at the Westin Peachtree Plaza Hotel. But there may be some members that we missed and feel it necessary to post a reminder for all who wish to attend our meeting. The deadline for the hotel reservations for the discounted rate is March 3rd. That rate is $129 single, double, triple or quad. The phone number to the Westin is 1-404-659-1400 and please state that you are attending the GSH/Region III meeting. The revised program can be downloaded from our website at www.histosearch.com/gsh. Click on the symposium link to get a PDF file of the program. Vendors have a link to the registration form to exhibit at the meeting on the same page. If you have questions Chris Coley, GSH Exhibit Liaison, has contact information is on that form If anyone has any questions please feel to contact me at this email address. Come on down to Georgia and experience some warmer weather. Shirley Powell GSH Secretary/Registrar From marktarango <@t> gmail.com Wed Feb 27 12:16:03 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Wed Feb 27 12:16:12 2008 Subject: [Histonet] Dull eosin? In-Reply-To: References: Message-ID: <5b6eb13e0802271016n5c1ac43bh9d09125b8bf02690@mail.gmail.com> Make sure you rinse well after the bluing with distilled water (you don't want to bring the pH up on the eosin from carry over of hard tap water or bluing reagent). Go into alcohol before eosin and don't do a water rinse after eosin. Make sure your first alcohol after eosin is 95% and fresh. On Wed, Feb 27, 2008 at 9:06 AM, Martin, Erin wrote: > Hi everyone, > > I am having a problem with my eosin in the H&E. My pathologists say that > it is dull and the RBCs are too red rather than orange. Interestingly, when > I take out the hematoxylin (Richard Allen Harris), acid acohol (Richard > Allen Clarifier) and bluing (Richard Allen Bluing) the eosin is nice and > bright. Our tap water for the rinses is pH 6.8, and we use a Sakura Prisma > stainer. Any ideas? > > Thank in advance! > > Erin Martin > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ASelf <@t> georgetownhospitalsystem.org Wed Feb 27 13:58:54 2008 From: ASelf <@t> georgetownhospitalsystem.org (Amy Self) Date: Wed Feb 27 13:59:03 2008 Subject: [Histonet] Premiere Microscope Slides Message-ID: Histonetters, Has anyone tried the Premiere Microscope Slides - charged - from Cardinal yet? If so how did you like them? Any feedback would be appreciated. Thanks, amy Amy Self Georgetown Hospital System NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From AnthonyH <@t> chw.edu.au Wed Feb 27 16:03:02 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Feb 27 16:03:52 2008 Subject: [Histonet] FISH cytology fixatives Message-ID: If you are looking for DNA translocations, 95% ethanol works well. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Wednesday, 27 February 2008 11:42 PM To: Histonet Subject: [Histonet] FISH cytology fixatives Hi Everyone, I am working up some FISH staining protocols for cytology (cell culture) slides (eventually to be OCT and FFPE tissues, but I need to walk before I can run). Most of the journals that I have read recommend using a fixative containing methanol and acetic acid (3:1). The DAKO probe I am using calls for the use of 3.7% fomaldehyde. I am wondering why acetone (or acetone/ethanol) is not the routine fixative for such a procedure. Can someone let me know why to choose one fixative over another and what the merits of methanol/glacial acetic acid would be? Thanks in advance, Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA --------------------------------- Never miss a thing. Make Yahoo your homepage. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From jnocito <@t> satx.rr.com Wed Feb 27 19:15:03 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Feb 27 19:15:11 2008 Subject: [Histonet] Dull eosin? References: Message-ID: <003a01c879a7$591e33b0$0302a8c0@yourxhtr8hvc4p> Erin, have you tried adding a couple of mls of glacial acetic acid to the Eosin container? JTT ----- Original Message ----- From: "Martin, Erin" To: "histonet" Sent: Wednesday, February 27, 2008 11:06 AM Subject: [Histonet] Dull eosin? Hi everyone, I am having a problem with my eosin in the H&E. My pathologists say that it is dull and the RBCs are too red rather than orange. Interestingly, when I take out the hematoxylin (Richard Allen Harris), acid acohol (Richard Allen Clarifier) and bluing (Richard Allen Bluing) the eosin is nice and bright. Our tap water for the rinses is pH 6.8, and we use a Sakura Prisma stainer. Any ideas? Thank in advance! Erin Martin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tjasper <@t> copc.net Wed Feb 27 19:41:08 2008 From: tjasper <@t> copc.net (Thomas Jasper) Date: Wed Feb 27 19:41:17 2008 Subject: [Histonet] New pan-cytokeratin monoclonal antibody References: <661949901A768E4F9CC16D8AF8F2838C017A372F@IS-E2K3.grhs.net> Message-ID: <90354A475B420441B2A0396E5008D4965E205E@copc-sbs.COPC.local> Hey Mike, We use it here and our docs are fond of it. We run it on the Ventana Benchmark XT, using UltraView detection. We're cool with it. Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Monday, February 25, 2008 6:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New pan-cytokeratin monoclonal antibody Hi All, Has anyone heard of or using the new OSCAR pan-cytokeratin monoclonal antibody from Signet Labs. I hear it does not have the same problems as the conventional pan-keratin stains. If you are using it, what system are you using it on? Thanks, Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jim.manavis <@t> imvs.sa.gov.au Wed Feb 27 20:41:10 2008 From: jim.manavis <@t> imvs.sa.gov.au (Jim Manavis) Date: Wed Feb 27 20:41:25 2008 Subject: [Histonet] hb crystals Message-ID: <002201c879b3$60615ec0$636c140a@itp36533> Dear Sir or Madam: I am a PhD student studying intracerebral haemorrhage in the rat and have found numerous crystals contained within the haemorrhage, which I presume are haemoglobin crystals, although it would be nice to demonstrate this. Pearl's stain doesn't work, presumably because the iron is ferrous and bound tightly, and Lillie's method with ferricyanide and HCL likewise doesn't work, presumably because the iron is bound. Our standard DAB technique for immunohistochemistry doesn't seem to react either. How might I best determine whether or not the crystals are haemoglobin crystals? Yours Sincerely, Tim Kleinig From histology.bc <@t> shaw.ca Thu Feb 28 00:25:02 2008 From: histology.bc <@t> shaw.ca (Paul Bradbury) Date: Thu Feb 28 00:21:09 2008 Subject: [Histonet] hb crystals In-Reply-To: <002201c879b3$60615ec0$636c140a@itp36533> References: <002201c879b3$60615ec0$636c140a@itp36533> Message-ID: <47C653BE.1050300@shaw.ca> Hi Jim, You have not described the appearance of the "crystals" contained within the area of the hemorrhage. But, I will go out on a limb here and guess that they are very dark (brown-black) and "spikey" with sharp points. If this is the case, what you are looking at is so called "formalin pigment". not hemoglobin pigment. This is an artefactual pigment produced by non-buffered (or inadequately buffered) formalin interacting with the hemoglobin in red cells. It is frequently seen in areas of hemorrhage or within any other red cell masses. You can confirm its identity by examining it using crossed polarizers; formalin pigment (acid formol hematin) will rotate the plane of polarized light and will appear bright white on a black background. It does not react with any other demonstration technique. It can be removed by soaking the dewaxed sections in saturated alcoholic picric acid for 30-60 minutes. If it is formalin pigment, your next step is to avoid it in future specimens. This can be easily done by using only buffered formalin solutions, pH 7.2 (either commercially prepared or following the directions found in any standard histology text). Prolonged exposure to formalin fixatives will increase the tendency to produce the pigment, even when the solutions are buffered. So, keep the fixation time to no more than 2-3 days. With rat brain (pretty small) this should be adequate time for thorough fixation. Paul Bradbury Kamloops, Canada Jim Manavis wrote: > Dear Sir or Madam: > > > > I am a PhD student studying intracerebral haemorrhage in the rat and have > found numerous crystals contained within the haemorrhage, which I presume > are haemoglobin crystals, although it would be nice to demonstrate this. > Pearl's stain doesn't work, presumably because the iron is ferrous and bound > tightly, and Lillie's method with ferricyanide and HCL likewise doesn't > work, presumably because the iron is bound. Our standard DAB technique for > immunohistochemistry doesn't seem to react either. How might I best > determine whether or not the crystals are haemoglobin crystals? > > > > Yours Sincerely, > > > > Tim Kleinig > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From marktarango <@t> gmail.com Thu Feb 28 00:26:07 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Feb 28 00:26:13 2008 Subject: [Histonet] hb crystals In-Reply-To: <002201c879b3$60615ec0$636c140a@itp36533> References: <002201c879b3$60615ec0$636c140a@itp36533> Message-ID: <5b6eb13e0802272226o2b54e65dg44a9b4e35a0d22ff@mail.gmail.com> Are you hitting them on the head? On Wed, Feb 27, 2008 at 6:41 PM, Jim Manavis wrote: > Dear Sir or Madam: > > > > I am a PhD student studying intracerebral haemorrhage in the rat and have > found numerous crystals contained within the haemorrhage, which I presume > are haemoglobin crystals, although it would be nice to demonstrate this. > Pearl's stain doesn't work, presumably because the iron is ferrous and > bound > tightly, and Lillie's method with ferricyanide and HCL likewise doesn't > work, presumably because the iron is bound. Our standard DAB technique for > immunohistochemistry doesn't seem to react either. How might I best > determine whether or not the crystals are haemoglobin crystals? > > > > Yours Sincerely, > > > > Tim Kleinig > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gu.lang <@t> gmx.at Thu Feb 28 01:19:56 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Feb 28 01:20:07 2008 Subject: AW: [Histonet] hb crystals In-Reply-To: <002201c879b3$60615ec0$636c140a@itp36533> Message-ID: <000001c879da$53287190$eeeea8c0@dielangs.at> If this crystals are formalin-pigment they have reducing capacitiy. Therefore with silverimpregnation techniques like Masson this would render black spots. Also the PAS stain would give positivity. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Jim Manavis Gesendet: Donnerstag, 28. Februar 2008 03:41 An: Histonet Betreff: [Histonet] hb crystals Dear Sir or Madam: I am a PhD student studying intracerebral haemorrhage in the rat and have found numerous crystals contained within the haemorrhage, which I presume are haemoglobin crystals, although it would be nice to demonstrate this. Pearl's stain doesn't work, presumably because the iron is ferrous and bound tightly, and Lillie's method with ferricyanide and HCL likewise doesn't work, presumably because the iron is bound. Our standard DAB technique for immunohistochemistry doesn't seem to react either. How might I best determine whether or not the crystals are haemoglobin crystals? Yours Sincerely, Tim Kleinig _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Andrew.Prior <@t> Smith-Nephew.com Thu Feb 28 02:22:36 2008 From: Andrew.Prior <@t> Smith-Nephew.com (Prior, Andrew) Date: Thu Feb 28 02:22:57 2008 Subject: [Histonet] RE: goldners trichrome In-Reply-To: <20080227180510.A02052B3C94E@spam.smith-nephew.com> References: <20080227180510.A02052B3C94E@spam.smith-nephew.com> Message-ID: <6C18ADDF244BF8439412C063019CFFEC04FEFF4C@EHS021.wound.san> Ricki, We've used this protocol in the past with some success on 15?m ground MMA sections. It was adapted from a Nottingham University method. As usual, you may need to tweak the times to suit your sections. Good Luck. Andrew Prior Histologist Smith &Nephew Research Centre York Science Park Heslington York YO10 5DF UK Staining Protocol: Solutions: * Ponceau/Acid Fuchsin Stock Solution: 1.5 g - Ponceau 2R. 0.5 g Acid Fuchsin. 2 ml Acetic Acid (glacial). 98 ml Distilled Water. * Azophloxine Stock Solution: 0.5 g Acid Red 1. 0.6 ml Acetic Acid (glacial). 99.4ml Distilled Water. * Ponceau Working Solution: 12ml Ponceau/Acid Fuchsin Stock Solution. 8ml Azophloxine Stock Solution. 80 ml 0.2% Acetic Acid. * Orange G Solution: 1 g Orange G. 1.5g Phosphomolybdic Acid. 250 ml Distilled Water. * Light Green Solution: 1 g Light Green (Yellowish). 1 ml Acetic Acid (glacial). 500 ml Distilled Water. Method: 1) Slides in distilled water for 15 minutes. 2) Stain in Weigert's Haematoxylin for 30 minutes. 3) Wash in running tap water for 2 minutes. 4) Differentiate in 0.5% acid alcohol (30 seconds). 5) Wash in water for 20 minutes. 6) Stain in Ponceau Working Solution for 5 minutes. 7) Rinse in 1% Acetic Acid for 15 seconds. 8) Stain in Orange G Solution for 20 minutes. 9) Rinse in 1% Acetic Acid for 10 seconds. 10) Stain in Light Green Solution for 20 minutes. 11) Rinse in water. 12) Blot dry. Expected Results: Nuclei = blue/black. Mineralised bone = green. Muscle = green. Osteoid = red. Collagen = red. >>>>>>>>>>>>>>>>>>>> -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: 27 February 2008 18:05 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 51, Issue 40 Message: 5 Date: Wed, 27 Feb 2008 10:19:52 -0500 From: Simoskevitz@Osteotech.com Subject: [Histonet] goldners trichrome To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Barbara, I was asked to do a Goldners Trichrome in Methymethacylate embedded bone . Do you have a good protocol for this or do you know where I could find one. Thank you for any help you can give me. Ricki Simoskevitz Osteotech Inc. Eatontown, NJ 07724 (732) 542-2800 X6328 **************** Confidentiality. This electronic transmission is strictly confidential to Smith & Nephew and intended solely for the addressee. It may contain information which is covered by legal, professional or other privilege. If you are not the intended addressee, or someone authorised by the intended addressee to receive transmissions on behalf of the addressee, you must not retain, disclose in any form, copy or take any action in reliance on this transmission. If you have received this transmission in error, please notify the sender as soon as possible and destroy this message. Smith & Nephew UK Limited Registered in England and Wales No.4421171 with registered office at 15 Adam Street, London WC2N 6LA From GenieJacobs <@t> texashealth.org Thu Feb 28 08:18:00 2008 From: GenieJacobs <@t> texashealth.org (Jacobs, Genie) Date: Thu Feb 28 08:18:14 2008 Subject: [Histonet] RE: Histonet Digest, Vol 51, Issue 40 In-Reply-To: References: Message-ID: <52CFD52DA9BBF340A87E1E58EF1DAF82108829@phdex02.txhealth.org> Kappa/lambda CISH geniejacobs) We are starting up Kappa lambda cish and would like any info on protocol and vendors Does anyone have any experience using the Histosonda probe made by CENBIMO? thanks -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, February 27, 2008 12:03 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 51, Issue 40 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. FISH cytology fixatives (Kim Merriam) 2. Re: FISH cytology fixatives (godsgalnow@aol.com) 3. Re: Fixing crab blood smear (Rene J Buesa) 4. RE: Night differential (Cheri Miller) 5. goldners trichrome (Simoskevitz@Osteotech.com) 6. Lyme Disease IHC/PCR (histosprv06@aol.com) 7. Histology Supervisor Position NY (rmweber113@comcast.net) 8. reference point slides. (Garry Ashton) 9. reference point slides. (Garry Ashton) 10. Dull eosin? (Martin, Erin) 11. RE: Dull eosin? (Mike Pence) 12. RE: Dull eosin? (Bartlett, Jeanine (CDC/CCID/NCZVED)) 13. FW: Region III Hosted by Georgia Society for Histotechnology (Shirley Powell) ---------------------------------------------------------------------- Message: 1 Date: Wed, 27 Feb 2008 04:42:25 -0800 (PST) From: Kim Merriam Subject: [Histonet] FISH cytology fixatives To: Histonet Message-ID: <668890.34731.qm@web50312.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Everyone, I am working up some FISH staining protocols for cytology (cell culture) slides (eventually to be OCT and FFPE tissues, but I need to walk before I can run). Most of the journals that I have read recommend using a fixative containing methanol and acetic acid (3:1). The DAKO probe I am using calls for the use of 3.7% fomaldehyde. I am wondering why acetone (or acetone/ethanol) is not the routine fixative for such a procedure. Can someone let me know why to choose one fixative over another and what the merits of methanol/glacial acetic acid would be? Thanks in advance, Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA --------------------------------- Never miss a thing. Make Yahoo your homepage. ------------------------------ Message: 2 Date: Wed, 27 Feb 2008 08:24:11 -0500 From: godsgalnow@aol.com Subject: Re: [Histonet] FISH cytology fixatives To: kmerriam2003@yahoo.com, histonet@lists.utsouthwestern.edu Message-ID: <8CA475B2C9387AD-804-4E7@FWM-M08.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" In Cytological preparation, one would use an acetic acid in the fixative to lyse the RBC's, which could ultimaetly interfere with the diagnosis.? Journal articles are great to help you research and give you a starting point for what it is you want to do, that is where I started.? But when you start doing the procedure, or playing with until you get it to work, you always need to start with the suggestions of the manufacturer of the probe.? That is how they validated it and it is known to work under those conditions.? At that point you can play with fixatives, if you need to. A lot of FISH testing requires post fixation anyway.? For, instance, the tissue comes in formalin (or whatever), but after you de-wax the slides you have to begin the pre-treatment, an most of the time there is a step in there that involves placing the slides in formalin (or whatever). Roxanne -----Original Message----- From: Kim Merriam To: Histonet Sent: Wed, 27 Feb 2008 7:42 am Subject: [Histonet] FISH cytology fixatives Hi Everyone, I am working up some FISH staining protocols for cytology (cell culture) slides (eventually to be OCT and FFPE tissues, but I need to walk before I can run). Most of the journals that I have read recommend using a fixative containing methanol and acetic acid (3:1). The DAKO probe I am using calls for the use of 3.7% fomaldehyde. I am wondering why acetone (or acetone/ethanol) is not the routine fixative for such a procedure. Can someone let me know why to choose one fixative over another and what the merits of methanol/glacial acetic acid would be? Thanks in advance, Kim Kim Merriam, MA, HT(ASCP) Cambridge, MA --------------------------------- Never miss a thing. Make Yahoo your homepage. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Wed, 27 Feb 2008 05:37:27 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Fixing crab blood smear To: "Cheah, Lit Chien" , histonet@lists.utsouthwestern.edu Message-ID: <927292.99610.qm@web61223.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Your procedure is standard, even for crab or spiny lobster for that matter (even when it really is hemolymph). They should be OK Do you know Dr. Phillips (working with spiny lobsters? I have lost contact with him since many years ago). Ren? J. Buesa "Cheah, Lit Chien" wrote: Dear All, I have been ask is there a batter way to fix crab blood smear before the slides travel 800 kilometers for us to do Giemsa Stain, appart from the normal air dry then fix in methanol and shipment. Big Thanks in advance from the largest State in Australia! Mr. Lit Chien CHEAH Histopathology Supervisor BSC (Medical Science) Animal Health Laboratories 3 Baron-Hay Court South Perth, 6155 WA. This e-mail and files transmitted with it are privileged and confidential information intended for the use of the addressee. The confidentiality and/or privilege in this e-mail is not waived, lost or destroyed if it has been transmitted to you in error. If you received this e-mail in error you must (a) not disseminate, copy or take any action in reliance on it; (b) please notify the Department of Agriculture and Food, WA immediately by return e-mail to the sender; (c) please delete the original e-mail. This email has been successfully scanned by McAfee Anti-Virus software. Department of Agriculture and Food WA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. ------------------------------ Message: 4 Date: Wed, 27 Feb 2008 08:22:25 -0600 From: "Cheri Miller" Subject: RE: [Histonet] Night differential To: "'Cindy DuBois'" , Message-ID: <001501c8794c$2cd6f720$3d02a8c0@plab.local> Content-Type: text/plain; charset="us-ascii" Where I used to work it was 15% of your hourly wage for any hours worked from 1100pm to 6am Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy DuBois Sent: Tuesday, February 26, 2008 10:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Night differential Are there any techs who get a night differential? If so, how much? We currently come to work @ 3:30 am. We are now being asked to come in at 1:30. I would like to ask for a night differential, but need to know how much to ask for. Thanks, Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ------------------------------ Message: 5 Date: Wed, 27 Feb 2008 10:19:52 -0500 From: Simoskevitz@Osteotech.com Subject: [Histonet] goldners trichrome To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Barbara, I was asked to do a Goldners Trichrome in Methymethacylate embedded bone . Do you have a good protocol for this or do you know where I could find one. Thank you for any help you can give me. Ricki Simoskevitz Osteotech Inc. Eatontown, NJ 07724 (732) 542-2800 X6328 ------------------------------ Message: 6 Date: Wed, 27 Feb 2008 10:29:35 -0500 From: histosprv06@aol.com Subject: [Histonet] Lyme Disease IHC/PCR To: histonet@lists.utsouthwestern.edu Message-ID: <8CA476CB1044DEB-80C-AE3@webmail-da16.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Good Morning everyone, no better place to come for advice than the histonet! Does anyone know of a facility where?we can send a processed skin block for Borrelia Burgdorferi via IHC and PCR as well as Erlichiosis via PCR? We are in Florida and are not having much luck finding?a place?that offers this testing.? We would greatly appreciate any knowledge you can provide. Thanks in advance. ? Kari Zajic, HT ------------------------------ Message: 7 Date: Wed, 27 Feb 2008 16:36:07 +0000 From: rmweber113@comcast.net Subject: [Histonet] Histology Supervisor Position NY To: histonet@lists.utsouthwestern.edu Message-ID: <022720081636.3418.47C591770005560100000D5A2215575114CCCECE9D0A0D0A99039D@comcast.net> Content-Type: text/plain I have a position for a Histology Supervisor available at an established GI group located in New City, NY, Rockland County. Candidate should have the knowledge to set up and maintain a state of the art facility. Knowledge of grossing, processing and routine histology of gastric biopsies required. $30.00/hr plus benefits. Qualified candidates can email me at rmweber113@comcast.net or call me at 732 814-1170 $500.00 for a successful referals committed for at least 90 days. Marilynn Weber H.T. (ASCP) QIHC Twincrest ------------------------------ Message: 8 Date: Wed, 27 Feb 2008 16:39:18 -0000 From: "Garry Ashton" Subject: [Histonet] reference point slides. To: , "histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi everyone, Has anybody used or had any experience of microscope slides with some type of reference point on them? Thanks in advance. Garry PICR UK -------------------------------------------------------- This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the University of Manchester. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. ------------------------------ Message: 9 Date: Wed, 27 Feb 2008 16:39:18 -0000 From: "Garry Ashton" Subject: [Histonet] reference point slides. To: , "histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi everyone, Has anybody used or had any experience of microscope slides with some type of reference point on them? Thanks in advance. Garry PICR UK -------------------------------------------------------- This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the University of Manchester. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. ------------------------------ Message: 10 Date: Wed, 27 Feb 2008 09:06:53 -0800 From: "Martin, Erin" Subject: [Histonet] Dull eosin? To: "histonet" Message-ID: Content-Type: text/plain; charset=iso-8859-1 Hi everyone, I am having a problem with my eosin in the H&E. My pathologists say that it is dull and the RBCs are too red rather than orange. Interestingly, when I take out the hematoxylin (Richard Allen Harris), acid acohol (Richard Allen Clarifier) and bluing (Richard Allen Bluing) the eosin is nice and bright. Our tap water for the rinses is pH 6.8, and we use a Sakura Prisma stainer. Any ideas? Thank in advance! Erin Martin ------------------------------ Message: 11 Date: Wed, 27 Feb 2008 11:13:35 -0600 From: "Mike Pence" Subject: RE: [Histonet] Dull eosin? To: "Martin, Erin" , "histonet" Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A373E@IS-E2K3.grhs.net> Content-Type: text/plain; charset="us-ascii" What eosin are you using? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Erin Sent: Wednesday, February 27, 2008 11:07 AM To: histonet Subject: [Histonet] Dull eosin? Hi everyone, I am having a problem with my eosin in the H&E. My pathologists say that it is dull and the RBCs are too red rather than orange. Interestingly, when I take out the hematoxylin (Richard Allen Harris), acid acohol (Richard Allen Clarifier) and bluing (Richard Allen Bluing) the eosin is nice and bright. Our tap water for the rinses is pH 6.8, and we use a Sakura Prisma stainer. Any ideas? Thank in advance! Erin Martin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Wed, 27 Feb 2008 12:22:28 -0500 From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" Subject: RE: [Histonet] Dull eosin? To: "Martin, Erin" , histonet Message-ID: <34BB307EFC9A65429BBB49E330675F72045E25FA@LTA3VS003.ees.hhs.gov> Content-Type: text/plain; charset=us-ascii I assume you are using the Richard Allan eosin Y alcoholic? Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin, Erin Sent: Wednesday, February 27, 2008 12:07 PM To: histonet Subject: [Histonet] Dull eosin? Hi everyone, I am having a problem with my eosin in the H&E. My pathologists say that it is dull and the RBCs are too red rather than orange. Interestingly, when I take out the hematoxylin (Richard Allen Harris), acid acohol (Richard Allen Clarifier) and bluing (Richard Allen Bluing) the eosin is nice and bright. Our tap water for the rinses is pH 6.8, and we use a Sakura Prisma stainer. Any ideas? Thank in advance! Erin Martin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Wed, 27 Feb 2008 12:25:09 -0500 From: Shirley Powell Subject: [Histonet] FW: Region III Hosted by Georgia Society for Histotechnology To: histonet@lists.utsouthwestern.edu Message-ID: <01MRSH6CO2WQ001E0L@Macon2.Mercer.edu> Content-Type: text/plain; charset=us-ascii I sent this out yesterday, but I never received it myself so this is just to make sure you guys get it. -----Original Message----- From: Shirley Powell [mailto:powell_sa@mercer.edu] Sent: Tuesday, February 26, 2008 4:48 PM To: 'histonet@lists.utsouthwestern.edu' Subject: Region III Hosted by Georgia Society for Histotechnology Hi Guys, We sent out over 1000 programs for the Region III meeting to be held in Atlanta Georgia April 3-5, 2008 at the Westin Peachtree Plaza Hotel. But there may be some members that we missed and feel it necessary to post a reminder for all who wish to attend our meeting. The deadline for the hotel reservations for the discounted rate is March 3rd. That rate is $129 single, double, triple or quad. The phone number to the Westin is 1-404-659-1400 and please state that you are attending the GSH/Region III meeting. The revised program can be downloaded from our website at www.histosearch.com/gsh. Click on the symposium link to get a PDF file of the program. Vendors have a link to the registration form to exhibit at the meeting on the same page. If you have questions Chris Coley, GSH Exhibit Liaison, has contact information is on that form If anyone has any questions please feel to contact me at this email address. Come on down to Georgia and experience some warmer weather. Shirley Powell GSH Secretary/Registrar ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 51, Issue 40 **************************************** The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From akbitting <@t> geisinger.edu Thu Feb 28 08:20:45 2008 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Thu Feb 28 08:27:53 2008 Subject: [Histonet] automated Elastic Staining Message-ID: <47C67CED.2B7F.00C9.0@geisinger.edu> I'm wondering if anyone is successfully using Ventanas automated Elastic Stain ? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From mona_diane <@t> hotmail.com Thu Feb 28 08:36:21 2008 From: mona_diane <@t> hotmail.com (Ramona Turner) Date: Thu Feb 28 08:36:26 2008 Subject: [Histonet] Try a different Eosin In-Reply-To: References: Message-ID: I was having alot of trouble with the Richard Allan eosin Y and the hematoxylin. They were not consistent anymore for some reason. I tried everything! I changed to Surgipath. They have a great new line of stains to replace the RA stains. My docs have not complained once since I switched. Also, there is no filtering required. Ramona Turner, HT Potomac Hospital Woodbridge, VA 22191 _________________________________________________________________ Shed those extra pounds with MSN and The Biggest Loser! http://biggestloser.msn.com/ From NHeath <@t> Lifespan.org Thu Feb 28 09:11:46 2008 From: NHeath <@t> Lifespan.org (Heath, Nancy L.) Date: Thu Feb 28 09:11:57 2008 Subject: [Histonet] Cytochrome Oxidase HELP Message-ID: <130E8991F210424096EFC6F42EA33B240224F2A1@LSCOEXCH1.lsmaster.lifespan.org> Hi All, Looking for any and all Cytochrome Oxidase/Cox procedures and jpegs (which would be really helpful) of muscle slides after staining with the COX procedure. My Neuro docs are driving me bananas by insisting that the procedure we use (which is a new procedure) is not "up to snuff"!? I have looked at the online neuromuscular site and my finished stained slides look like what is on that site!! HELP ME PLEASE!!! Ready to make cocktails with my ethyl alcohol!! Nancy From cad <@t> Stowers-Institute.org Thu Feb 28 09:27:24 2008 From: cad <@t> Stowers-Institute.org (Dickey, Coral) Date: Thu Feb 28 09:27:40 2008 Subject: [Histonet] Zebrafish histology Message-ID: Hello out there, I am doing double skeletal staining on zebrafish and we run into a problem with visualization of th skeleton. The scales take up the alizarin red stain and they are incredibly persistent and don't come off the fish very well even after weeks in KOH/Glycerin solution. They make it difficult to see the inside of the fish. The rest of the fish is quite delicate after this length of time however, posing a challenge to remove the scales without breaking the fish to pieces! The first time I performed this stain, I used a plastic pipette to debride the scales. This was incredibly tedious and time consuming. Does anyone have experience with this or even have a good idea? Any suggestions appreciated! Thanks in advance, Coral Dickey Histology Specialist I Stowers Institute for Medical Research 1000 E. 50th Street Kansas City, Missouri 64110 Phone: 816-926-4305 e-mail: cad@stowers-institute.org From arsenn <@t> hsh.org Thu Feb 28 09:32:31 2008 From: arsenn <@t> hsh.org (Senn, Amy R) Date: Thu Feb 28 09:32:39 2008 Subject: [Histonet] Strange Coplin Jars Message-ID: Hi all, We are looking for plastic coplin jars. I think the manufacturer was Parkway.... They are not the 'standard' coplin jars we all know and love; they are about ? that size, with a red insert that holds slides. This insert is removable...we like these because if we have to put the jars under our faucet, they stay put, where the taller one slide all over the sink. I've searched for Parkway and all sorts of coplin jars online, but can't find anything even similar. Maybe they aren't manufacturing these anymore? Thanks for any information you can give me! Have a great week!!!! Amy Histotech, Histology Laboratory Holy Spirit Hospital 503 N. 21st Street Camp Hill, PA 17011 (717) 763-2124 Cell: (724) 494-2237 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You From gu.lang <@t> gmx.at Thu Feb 28 09:48:10 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Feb 28 09:48:19 2008 Subject: AW: [Histonet] automated Elastic Staining In-Reply-To: <47C67CED.2B7F.00C9.0@geisinger.edu> Message-ID: <001501c87a21$52680f80$eeeea8c0@dielangs.at> We used this staining kit, but not successfully. Now we do the stain per hand again. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Angela Bitting Gesendet: Donnerstag, 28. Februar 2008 15:21 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] automated Elastic Staining I'm wondering if anyone is successfully using Ventanas automated Elastic Stain ? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From rjbuesa <@t> yahoo.com Thu Feb 28 10:20:36 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 28 10:20:40 2008 Subject: [Histonet] Zebrafish histology In-Reply-To: Message-ID: <237110.90914.qm@web61216.mail.yahoo.com> If you want to able to see the internal organs and the skeleton, why don't completely make the fish transparent? To do that dehydrate in ethanol of increasing strengths, slowly and after the fish are dehydrated, clear them with cedar wood oil. It will take some time but you will end with fish completely cleared. Ren? J. "Dickey, Coral" wrote: Hello out there, I am doing double skeletal staining on zebrafish and we run into a problem with visualization of th skeleton. The scales take up the alizarin red stain and they are incredibly persistent and don't come off the fish very well even after weeks in KOH/Glycerin solution. They make it difficult to see the inside of the fish. The rest of the fish is quite delicate after this length of time however, posing a challenge to remove the scales without breaking the fish to pieces! The first time I performed this stain, I used a plastic pipette to debride the scales. This was incredibly tedious and time consuming. Does anyone have experience with this or even have a good idea? Any suggestions appreciated! Thanks in advance, Coral Dickey Histology Specialist I Stowers Institute for Medical Research 1000 E. 50th Street Kansas City, Missouri 64110 Phone: 816-926-4305 e-mail: cad@stowers-institute.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From PMonfils <@t> Lifespan.org Thu Feb 28 10:36:27 2008 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Feb 28 10:36:33 2008 Subject: [Histonet] Oil of Cajeput?? Message-ID: <4EBFF65383B74D49995298C4976D1D5E273D28@LSRIEXCH1.lsmaster.lifespan.org> I have a request to do a cresyl echt violet stain for nerve cell differentiation. The protocol calls for oil of cajeput. Has anyone used this? Know where to get it? Or, has anyone done this technique and know of a suitable substitute for this oil? Thanks. From ploykasek <@t> phenopath.com Thu Feb 28 10:44:52 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Thu Feb 28 10:45:11 2008 Subject: [Histonet] Oil of Cajeput?? In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E273D28@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: This is also known as tea tree oil. It is readily available at most natural health/organic stores (& we have a plethora of those stores in the Northwest!). I'm sure you could substitute something else, but I am not familiar with this stain. Patti Loykasek > I have a request to do a cresyl echt violet stain for nerve cell > differentiation. The protocol calls for oil of cajeput. Has anyone used > this? Know where to get it? Or, has anyone done this technique and know of a > suitable substitute for this oil? Thanks. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From bhewlett <@t> cogeco.ca Thu Feb 28 10:52:39 2008 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Thu Feb 28 10:53:17 2008 Subject: [Histonet] Oil of Cajeput?? References: <4EBFF65383B74D49995298C4976D1D5E273D28@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <000301c87a2a$59ae4f30$6500a8c0@mainbox> Yes, many years ago! It's extracted from the leaves and twigs of the Cajeput tree (Melaleuca quinquenervia) and sometimes used as an insect repellent. You could substitute the similar tea tree oil (Melaleuca leucadendron). Try your local health food store or Dentist(used for dry sockets). Bryan ----- Original Message ----- From: "Monfils, Paul" To: Sent: Thursday, February 28, 2008 11:36 AM Subject: [Histonet] Oil of Cajeput?? I have a request to do a cresyl echt violet stain for nerve cell differentiation. The protocol calls for oil of cajeput. Has anyone used this? Know where to get it? Or, has anyone done this technique and know of a suitable substitute for this oil? Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Flynn.Kevin <@t> epamail.epa.gov Thu Feb 28 11:00:45 2008 From: Flynn.Kevin <@t> epamail.epa.gov (Flynn.Kevin@epamail.epa.gov) Date: Thu Feb 28 11:01:46 2008 Subject: [Histonet] Zebrafish histology In-Reply-To: <237110.90914.qm@web61216.mail.yahoo.com> Message-ID: Another technique that we've used to clear fish (in our case medaka) is to clear into immersion oil but instead of oil with a refractice index of 1.515 (typical immersion oil) we found much better results using oil with a refractive index of 1.56. You can get the oil from Cargille. To clear, pass the fish from fixation (aqueous) to methanol then to propylene oxide then to immersion oil. I've never tried it with the skeletal stains. I can give you a more detailed protocol if interested. Kevin Flynn US EPA Mid-Continent Ecological Division 6201 Congdon Blvd Duluth MN 55804 218.529.5120 Rene J Buesa To Sent by: "Dickey, Coral" histonet-bounces , @lists.utsouthwe "histonet@lists.utsouthwestern.ed stern.edu u" 02/28/2008 10:20 cc AM Subject Re: [Histonet] Zebrafish histology If you want to able to see the internal organs and the skeleton, why don't completely make the fish transparent? To do that dehydrate in ethanol of increasing strengths, slowly and after the fish are dehydrated, clear them with cedar wood oil. It will take some time but you will end with fish completely cleared. Ren? J. "Dickey, Coral" wrote: Hello out there, I am doing double skeletal staining on zebrafish and we run into a problem with visualization of th skeleton. The scales take up the alizarin red stain and they are incredibly persistent and don't come off the fish very well even after weeks in KOH/Glycerin solution. They make it difficult to see the inside of the fish. The rest of the fish is quite delicate after this length of time however, posing a challenge to remove the scales without breaking the fish to pieces! The first time I performed this stain, I used a plastic pipette to debride the scales. This was incredibly tedious and time consuming. Does anyone have experience with this or even have a good idea? Any suggestions appreciated! Thanks in advance, Coral Dickey Histology Specialist I Stowers Institute for Medical Research 1000 E. 50th Street Kansas City, Missouri 64110 Phone: 816-926-4305 e-mail: cad@stowers-institute.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ian.montgomery <@t> bio.gla.ac.uk Thu Feb 28 11:01:17 2008 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Thu Feb 28 11:08:43 2008 Subject: FW: [Histonet] Oil of Cajeput?? Message-ID: <009501c87a2b$88743a40$6424d182@IBLS.GLA.AC.UK> You're taking me back to the 1960's with this one. If you were near Glasgow I would give you a bottle. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, G12 8QQ. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: 28 February 2008 16:36 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Oil of Cajeput?? I have a request to do a cresyl echt violet stain for nerve cell differentiation. The protocol calls for oil of cajeput. Has anyone used this? Know where to get it? Or, has anyone done this technique and know of a suitable substitute for this oil? Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kellerc2 <@t> uthscsa.edu Thu Feb 28 11:11:23 2008 From: kellerc2 <@t> uthscsa.edu (Keller, Charles) Date: Thu Feb 28 11:11:31 2008 Subject: [Histonet] Zebrafish histology In-Reply-To: References: <237110.90914.qm@web61216.mail.yahoo.com> Message-ID: microCT-based virtual histology works pretty well for this purpose, too. Resolution is limited to 1 um. See this image at http://www.numirabio.com/gallery/fullsize/zebrafish1.PNG as well as others at http://www.numirabio.com/gallery.html . My disclaimer is that I co-founded the company that does this imaging. I'd be happy to do it in my academic lab, though, at no cost. Charles Charles Keller, MD Assistant Professor, Department of Cellular & Structural Biology Adjunct Assistant Professor, Department of Pediatrics Director, Small Animal Imaging Facility Greehey Children's Cancer Research Institute The University of Texas Health Science Center 8403 Floyd Curl Drive, Mail Code 7784 San Antonio, TX 78229-3900 210-562-9062 [office] 210-562-9014 [fax] http://gccri.uthscsa.edu or www.sarcomalab.org kellerc2@uthscsa.edu Postdoctoral Opportunities and Undergraduate & Summer Internships: http://ccri.uthscsa.edu/keller -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Flynn.Kevin@epamail.epa.gov Sent: Thursday, February 28, 2008 11:01 AM To: Dickey, Coral Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Zebrafish histology Another technique that we've used to clear fish (in our case medaka) is to clear into immersion oil but instead of oil with a refractice index of 1.515 (typical immersion oil) we found much better results using oil with a refractive index of 1.56. You can get the oil from Cargille. To clear, pass the fish from fixation (aqueous) to methanol then to propylene oxide then to immersion oil. I've never tried it with the skeletal stains. I can give you a more detailed protocol if interested. Kevin Flynn US EPA Mid-Continent Ecological Division 6201 Congdon Blvd Duluth MN 55804 218.529.5120 Rene J Buesa To Sent by: "Dickey, Coral" histonet-bounces , @lists.utsouthwe "histonet@lists.utsouthwestern.ed stern.edu u" 02/28/2008 10:20 cc AM Subject Re: [Histonet] Zebrafish histology If you want to able to see the internal organs and the skeleton, why don't completely make the fish transparent? To do that dehydrate in ethanol of increasing strengths, slowly and after the fish are dehydrated, clear them with cedar wood oil. It will take some time but you will end with fish completely cleared. Ren? J. "Dickey, Coral" wrote: Hello out there, I am doing double skeletal staining on zebrafish and we run into a problem with visualization of th skeleton. The scales take up the alizarin red stain and they are incredibly persistent and don't come off the fish very well even after weeks in KOH/Glycerin solution. They make it difficult to see the inside of the fish. The rest of the fish is quite delicate after this length of time however, posing a challenge to remove the scales without breaking the fish to pieces! The first time I performed this stain, I used a plastic pipette to debride the scales. This was incredibly tedious and time consuming. Does anyone have experience with this or even have a good idea? Any suggestions appreciated! Thanks in advance, Coral Dickey Histology Specialist I Stowers Institute for Medical Research 1000 E. 50th Street Kansas City, Missouri 64110 Phone: 816-926-4305 e-mail: cad@stowers-institute.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mwich <@t> 7thwavelabs.com Thu Feb 28 11:17:31 2008 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Thu Feb 28 11:17:39 2008 Subject: [Histonet] Safranin O in GMA Message-ID: <2264717ADC396742A0FF0AAB674F9A0D486BBB@7THWAVE-SERVER.7thwave.local> Does anyone know if special stains can be done on GMA-embedded bone sections? More specifically, does anyone have a protocol for (or any insight into) doing the Safranin O stain on this? Thanks in advance for any advice. ~Michele This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From llewllew <@t> shaw.ca Thu Feb 28 11:29:59 2008 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Thu Feb 28 11:30:09 2008 Subject: [Histonet] Oil of Cajeput?? References: <4EBFF65383B74D49995298C4976D1D5E273D28@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <001201c87a2f$8b202390$bd144246@yourlk4rlmsu> I used to love the smell of cajaput oil when I was a junior tech in England. You can stain nerve cells, actually Nissl, without it. 1. Overstain with the cresyl fast violet. 2. Without any differentiation, dehydrate thoroughly and clear in xylene. 3. Leave it there at least half an hour, possibly overnight. 4. Bring it back to absolute ethanol. 5. Differentiate with absolute ethanol containing a few drops of acetic acid per 100 mL until stained as you want. 6. Wash well with absolute ethanol to remove all traces of acetic acid. 7. Clear with xylene again, and mount. It is commonly observed that the initial dehydration and clearing improves the later differentiation, but I don't know why. Bryan Llewellyn ----- Original Message ----- From: "Monfils, Paul" To: Sent: Thursday, February 28, 2008 8:36 AM Subject: [Histonet] Oil of Cajeput?? I have a request to do a cresyl echt violet stain for nerve cell differentiation. The protocol calls for oil of cajeput. Has anyone used this? Know where to get it? Or, has anyone done this technique and know of a suitable substitute for this oil? Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dencrowl <@t> MIT.EDU Thu Feb 28 11:39:22 2008 From: dencrowl <@t> MIT.EDU (Denise Crowley) Date: Thu Feb 28 11:39:30 2008 Subject: [Histonet] Premiere microscope slides Message-ID: <1A12C5A5-84A2-4CE9-BD7C-E417082BDB79@mit.edu> Message: 2 Date: Wed, 27 Feb 2008 14:58:54 -0500 From: "Amy Self" Subject: [Histonet] Premiere Microscope Slides To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Histonetters, Has anyone tried the Premiere Microscope Slides - charged - from Cardinal yet? If so how did you like them? Any feedback would be appreciated. Thanks, amy Amy Self Georgetown Hospital System Hi Amy, We have been using the Premiere slides, both charged and uncharged, for about 6 months now. We purchase them from Mercedes Medical. The difference in price between these and our old slides is amazing (just about 1/3 the cost). The only complaint that I have with them is that they are about 1/16th of an inch short of 3 inches. This becomes an issue because we cut sections and dry them on edge in metal racks. So one end of the slide is held in place, but the other doesn't quite meet the metal designed to hold it in place. We adjusted to this by squeezing the sides of the rack, making the sides of the rack bow in a bit. I don't know how much longer we will be using them, however. We have just purchased a new slide writer and it is recommended to use clipped corner slides with this instrument. The Premiere slides have a small clipped corner, but I am not sure if we will have to go to a more expensive slide to get the best performance from the slide writer. Hope this helps to answer your questions. Denise Crowley Facility Manager Histology Center for Cancer Research Massachusetts Institute of Technology 40 Ames St. E17-230 Cambridge MA 02139 617-258-8183 dencrowl@mit.edu From JWEEMS <@t> sjha.org Thu Feb 28 11:42:54 2008 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Thu Feb 28 11:43:00 2008 Subject: [Histonet] Premiere microscope slides In-Reply-To: <1A12C5A5-84A2-4CE9-BD7C-E417082BDB79@mit.edu> References: <1A12C5A5-84A2-4CE9-BD7C-E417082BDB79@mit.edu> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD320518E7FA@sjhaexc02.sjha.org> We use these also - in our Sakura slide writer...j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Denise Crowley Sent: Thursday, February 28, 2008 12:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Premiere microscope slides Message: 2 Date: Wed, 27 Feb 2008 14:58:54 -0500 From: "Amy Self" Subject: [Histonet] Premiere Microscope Slides To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Histonetters, Has anyone tried the Premiere Microscope Slides - charged - from Cardinal yet? If so how did you like them? Any feedback would be appreciated. Thanks, amy Amy Self Georgetown Hospital System Hi Amy, We have been using the Premiere slides, both charged and uncharged, for about 6 months now. We purchase them from Mercedes Medical. The difference in price between these and our old slides is amazing (just about 1/3 the cost). The only complaint that I have with them is that they are about 1/16th of an inch short of 3 inches. This becomes an issue because we cut sections and dry them on edge in metal racks. So one end of the slide is held in place, but the other doesn't quite meet the metal designed to hold it in place. We adjusted to this by squeezing the sides of the rack, making the sides of the rack bow in a bit. I don't know how much longer we will be using them, however. We have just purchased a new slide writer and it is recommended to use clipped corner slides with this instrument. The Premiere slides have a small clipped corner, but I am not sure if we will have to go to a more expensive slide to get the best performance from the slide writer. Hope this helps to answer your questions. Denise Crowley Facility Manager Histology Center for Cancer Research Massachusetts Institute of Technology 40 Ames St. E17-230 Cambridge MA 02139 617-258-8183 dencrowl@mit.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From cmiller <@t> physlab.com Thu Feb 28 11:55:12 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Thu Feb 28 11:55:27 2008 Subject: [Histonet] FW: under processed tissue Message-ID: <000001c87a33$10a775b0$3d02a8c0@plab.local> Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 _____ From: Cheri Miller [mailto:cmiller@physlab.com] Sent: Thursday, February 28, 2008 11:31 AM To: histonet-bounces@lists.utsouthwestern.edu Subject: FW: under processed tissue Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 _____ From: Cheri Miller [mailto:cmiller@physlab.com] Sent: Thursday, February 28, 2008 11:01 AM Cc: 'mfokol@sakuraus.com' Subject: under processed tissue Hi, I hope someone out there can help me. I am having issues with under-processed tissue. A few weeks ago we had power outages and our VIP 2000 battery backup didn't work. We have since fixed this problem and everything on the VIP is working as before. My problem is I had to set up my processing programs again.( the programs were set prior to my employment) I suspect my times are not the same in each station as before and are causing the subtle and not so subtle changes I am seeing. Every thing is cutting well except the more fatty tissues (breasts, colons, lipomas etc.) I have listed my routine program below. I forgot to mention that we put our fatty tissues in lilies fix (formaldehyde, alcohol and acetic acid) For 3-5 hours prior to processing. Station Fluid Time 1 Formalin 10% 60 mins 2 Formalin 10% 60 mins 3 70% Reagent alcohol 45 mins 4 95% Reagent alcohol 50 mins 5 95% Reagent alcohol 50 mins 6 100% Reagent alcohol 50 mins 7 100% Reagent alcohol 50 mins 8 100 % Reagent alcohol 50 mins 9 Xylene 45 mins 10 Xylene 45 mins 11 Empty/ paraffin bath 0 12 Paraffin 30 mins 13 Paraffin 30 mins 14 Paraffin 45 mins 15 Clean xylene 16 Clean alcohol Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From lgruman <@t> mcw.edu Thu Feb 28 12:16:12 2008 From: lgruman <@t> mcw.edu (Gruman, Lynn) Date: Thu Feb 28 12:15:52 2008 Subject: [Histonet] Whole mounting of rats Message-ID: Is there anyone in histoland that performs whole mounting of rats? We have an investigator who is interested in performing this technique. If you could contact me so I can give you the PI's information I would greatly appreciated it. Sincerely, Lynn Gruman, HT(ASCP) Program Coordinator II Histology and Imaging Core Medical College of Wisconsin 8701 Watertown Plank Road CRI/TBRC Building 4th Floor/ C4470 Milwaukee, WI 53226 Phone: 414-955-8624 Fax: 414-955-6411 Email: lgruman@mcw.edu From rjbuesa <@t> yahoo.com Thu Feb 28 12:37:23 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Feb 28 12:37:37 2008 Subject: [Histonet] FW: under processed tissue In-Reply-To: <000001c87a33$10a775b0$3d02a8c0@plab.local> Message-ID: <252083.52274.qm@web61222.mail.yahoo.com> Cheri: Reduce the alcohols times and increase the xylene times. Fat tissue contains less water than other tissues so they don't need prolongues dehydraitons but need more time in the antemedium (xylene) to aliminate the fat and allow the paraffin to infiltrate. Ren? J. Cheri Miller wrote: --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From lhunt <@t> uta.edu Thu Feb 28 12:39:30 2008 From: lhunt <@t> uta.edu (Laura Hunt) Date: Thu Feb 28 12:39:38 2008 Subject: [Histonet] Azure II/Basic Fuschin question In-Reply-To: References: Message-ID: <0674161B-CA56-4788-9D0C-7AA79BA7FA79@uta.edu> Hi, I have yet another question for you histo experts! I I have made Azure II and Basic Fuschin from some pretty old powders that were given to me. So far, I have tested them out on one slide with poor results. Do these need to be filtered? Also, can anyone recommend a recipe for Azure II and Basic Fuschin? I will compare to mine. Thanks!!! LH From lpwenk <@t> sbcglobal.net Thu Feb 28 12:54:43 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Feb 28 12:54:51 2008 Subject: [Histonet] Oil of Cajeput?? In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E273D28@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <002401c87a3b$61ad36e0$0202a8c0@HPPav2> Here's our procedure for Cresyl echt violet (which, by the way, as a dye - no longer exists. Use cresyl violet acetate): REAGENTS: CRESYL ECHT VIOLET STAINING SOLUTION 0.5 g Cresyl echt violet (Cresyl Violet Acetate) 0.18 g Sodium acetate (CH3COONaC3H2O) 500 mL Distilled water Acetic acid, concentrated (CH3COOH) 1.5 mL (approximately - see instructions) Dissolve cresyl echt violet and sodium acetate in distilled water. Slowly add acetic acid, drop by drop, to solution. Should have a pH of 3.5. If solution pH is below 3.5, add more sodium acetate. If solution pH is above 3.5, add more acetic acid. Filter. Let stand overnight before using. Store at room temperature. Stable for months. May be reused until weak. PROCEDURE - Cresyl Echt Violet: 1. Deparaffinize and hydrate slides through graded alcohol to distilled water. 2. Place sections in cresyl echt violet solution 1-2 hours (up to overnight) at room temperature. 3. Differentiate in two changes of 95% ethanol until nuclei and Nissl granules remain violet and the background is nearly colorless. Check differentiation with the microscope. 1 to 2 drops of acetic acid many be added to the first alcohol to speed up differentiation. 4. Dehydrate through absolute ethanol and clear in xylene. 5. Coverslip with a synthetic mounting media. RESULTS: Nissl granules - violet Nuclei - violet Bacteria, fungus - blue to purple Cartilage, mast cell granules - blue to purple Background - colorless to a very very pale violet-gray PROCEDURAL NOTES: 1. Use the microscope to differentiate. Differentiations may need to be repeated several times. 2. May be used as a counterstain after the Luxol fast blue procedure. a. AFTER LFB: Start after the LFB has been differentiated and is still in d. water, but before it is run up through alcohols into xylene. b. CEV STAIN: Since slides are in d. water after LFB, slides can go immediately into CEV staining solution. 3. To demonstrate only Nissl substance, continue to differentiate until the nuclei are colorless. Definitely add the acetic acid to the differentiating alcohols. 4. Cresyl echt violet dye powder is no longer available. (German dye discontinued in the 1940's.) Substitute cresyl violet acetate (no Color Index number). Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Thursday, February 28, 2008 11:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Oil of Cajeput?? I have a request to do a cresyl echt violet stain for nerve cell differentiation. The protocol calls for oil of cajeput. Has anyone used this? Know where to get it? Or, has anyone done this technique and know of a suitable substitute for this oil? Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Thu Feb 28 13:06:01 2008 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Feb 28 13:06:09 2008 Subject: [Histonet] Oil of Cajeput Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E61B7@nmdamailsvr.nmda.ad.nmsu.edu> I think King Arthur used the last bottle pulling Excalibur from the stone... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From angela.mcnabola <@t> ikonisys.com Thu Feb 28 13:17:01 2008 From: angela.mcnabola <@t> ikonisys.com (Angela McNabola) Date: Thu Feb 28 13:17:08 2008 Subject: [Histonet] Destaining PAP slides In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E61B7@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <4ECD18F12E443644B1F3C924A20398246B9A03@ikoexchange.ikonisys.com> Good Afternoon, Does anyone out there have a good protocol for destaining PAP slides? I'm really trying to remove not only the color, but the fluorescence caused by the stain itself. Thanks Angela Angela McNabola, MS HT(ASCP)SLS, QIHC Research Scientist Ikonisys, Inc. 5 Science Park New Haven, CT 06511 203-776-0791 angela.mcnabola@ikonisys.com From lpwenk <@t> sbcglobal.net Thu Feb 28 13:39:32 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Feb 28 13:39:39 2008 Subject: [Histonet] Destaining PAP slides In-Reply-To: <4ECD18F12E443644B1F3C924A20398246B9A03@ikoexchange.ikonisys.com> Message-ID: <002801c87a41$a4adb6d0$0202a8c0@HPPav2> I would guess a lot of the autofluorescence would be due to the eosin. Are you seening a greenish color? Hematoxylin would actually be blocking the nuclei autofluorescence. So the cytoplasm would be green glowing, with black holes for nuclei. Try decolorizing like an H&E. Remove the coverslip. Remove the mounting media with 3 changes xylene or substitute, about 5 minutes each. 2 changes absolute alcohol, 1 minute each 2 changes 95% alcohol, 1 minute each 1 change 70% alcohol, 1 minute Rinse in running water, 1 minute - (running down through alcohol and going into water should get rid a lot of the light green and some of the eosin) Place in 1% acetic acid (or if you use a regressive H&E, use the acid rinse after the hematoxylin). This should remove the hematoxylin. Dip up and down for 5-10 seconds, up to 1 minute - check with a microscope. Rinse in water 1 minute. Place in the bluing agent used in the H&E (ammonia water, lithium carbonate, etc.). 5-60 seconds. Check with microcope. This should remove the eosin, and probably the orange G. Rinse in water 1 minute. Should be set to go. If your formulation has Bismarck brown, I don't know how to remove it, simply because I never understood how or if it was working in the Pap stain. It's at the wrong pH - it's in a acidic solution, but it needs a basic pH to bind to tissue. But, since you are going into - water (which removes dyes soluble in water) - alcohol (which removes dyes soluble in alcohol) - dilute acid (which removes basic/cationic dyes) - dilute base (which removes acidic/anionic dyes) I think we have removed just about every variation of dye there is found in a Pap stain. Hope that helps. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela McNabola Sent: Thursday, February 28, 2008 2:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Destaining PAP slides Good Afternoon, Does anyone out there have a good protocol for destaining PAP slides? I'm really trying to remove not only the color, but the fluorescence caused by the stain itself. Thanks Angela Angela McNabola, MS HT(ASCP)SLS, QIHC Research Scientist Ikonisys, Inc. 5 Science Park New Haven, CT 06511 203-776-0791 angela.mcnabola@ikonisys.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tim.morken <@t> thermofisher.com Thu Feb 28 14:10:13 2008 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Thu Feb 28 14:10:49 2008 Subject: [Histonet] Dorothy Ip of Ohio? Message-ID: <6BFF6D137DF6BC43B33891BA96E83B190134E05D@PGHCR-EXMB-VS-1.na.fshrnet.com> I am trying to get a hold of Dorothy Ip of Ohio. Any help will be appreciated. Tim Morken Technical Support Manager Lab Vision Products Anatomical Pathology ThermoFisher Scientific 47777 Warm Springs Blvd. Fremont, CA 94539 USA tel: 01-510-991-2840 fax: 01 510-991-2826 Tim.Morken@thermofisher.com web: www.labvision.com www.thermofisher.com The World Leader in Serving Science From scoop <@t> mail.nih.gov Thu Feb 28 14:52:01 2008 From: scoop <@t> mail.nih.gov (scoop@mail.nih.gov) Date: Thu Feb 28 14:52:49 2008 Subject: [Histonet] Oil of Cajeput In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E61B7@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B017E61B7@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: I think it's the same as tea tree oil - you can get it at a health food store. I think it's used as a mosquito repellent and for skin or something. >I think King Arthur used the last bottle pulling Excalibur from the >stone... > > > >Sally Breeden, HT(ASCP) > >NM Dept. of Agriculture > >Veterinary Diagnostic Services > >PO Box 4700 > >Albuquerque, NM 87106 > >505-841-2576 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lnlj <@t> novonordisk.com Thu Feb 28 15:32:58 2008 From: lnlj <@t> novonordisk.com (LNLJ (Lene Lyngsie Jensen)) Date: Thu Feb 28 15:33:08 2008 Subject: [Histonet] Please unsubmit - thank you :) In-Reply-To: Message-ID: From azdudley <@t> hotmail.com Thu Feb 28 15:37:53 2008 From: azdudley <@t> hotmail.com (anita dudley) Date: Thu Feb 28 15:38:01 2008 Subject: [Histonet] qc testing detection In-Reply-To: <400.22154.qm@web61223.mail.yahoo.com> References: <47C431C6.2B7F.00C9.0@geisinger.edu> <400.22154.qm@web61223.mail.yahoo.com> Message-ID: is anyone using ruo's in their labs, other than research. and what do you put on the report if you are using them? thanks anita [Histonet] qc testing detection> CC: > > At least try the new lot with the usual (+) control with the usual protocol and if everything turns as expected, document that test.> IF it does not react as expected, then you will have to "start all over again" with the required checker board tests, etc.> Ren? J.> > Angela Bitting wrote:> Hello Histonetters,> > What methods are people using to validate new lots of antibody and detection before they are put into use? Do you incorporate them into your normal work flow, or do you do QC runs? What batteries of tissue do you use? Just curious what others are doing.> > Thanks all!> > Angela Bitting, HT(ASCP)> Technical Specialist, Histology> Geisinger Medical Center > 100 N Academy Ave. MC 23-00> Danville, PA 17822> phone 570-214-9634> fax 570-271-5916 > > > > IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD> VERSION:2.1> X-GWTYPE:USER> FN:Bitting, Angela> TEL;WORK:570-271-6844> ORG:;Histology> EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu> N:Bitting;Angela> END:VCARD> > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > ---------------------------------> Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now.> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Helping your favorite cause is as easy as instant messaging.?You IM, we give. http://im.live.com/Messenger/IM/Home/?source=text_hotmail_join From talulahgosh <@t> gmail.com Thu Feb 28 15:48:54 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Feb 28 15:49:00 2008 Subject: [Histonet] Oil of Cajeput In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E61B7@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B017E61B7@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: Sweet! I'm adapting this phrase whenever my boss wants to do something archaic. On Thu, Feb 28, 2008 at 2:06 PM, Breeden, Sara wrote: > I think King Arthur used the last bottle pulling Excalibur from the > stone... > > Emily -- fortune smiles on the brave and spits on the coward --aguirre, wrath of god From talulahgosh <@t> gmail.com Thu Feb 28 16:09:11 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Thu Feb 28 16:09:23 2008 Subject: [Histonet] cryostat question Message-ID: Any opinions on Leica's newest model, CM1950? I can't figure out how one could keep a vacuum in a cryostat that's being used. Emily -- fortune smiles on the brave and spits on the coward --aguirre, wrath of god From histology.bc <@t> shaw.ca Thu Feb 28 18:27:26 2008 From: histology.bc <@t> shaw.ca (Paul Bradbury) Date: Thu Feb 28 18:23:26 2008 Subject: AW: [Histonet] hb crystals In-Reply-To: <000001c879da$53287190$eeeea8c0@dielangs.at> References: <000001c879da$53287190$eeeea8c0@dielangs.at> Message-ID: <47C7516E.5070705@shaw.ca> I have never seen the PAS reaction give positive results with formalin pigment. Even if it did react, how would it be seen. The pigment is already dark brown-black, so colouring it red would have no visible effect. The same problem applies to any silver methods which might work ... it's already almost black, so making it a "darker black" would not be much help. The only definitive way to identify it is to use its birefringent properties. Paul Kamloops, Canada Gudrun Lang wrote: > If this crystals are formalin-pigment they have reducing capacitiy. > Therefore with silverimpregnation techniques like Masson this would render > black spots. Also the PAS stain would give positivity. > > Gudrun Lang > > Biomed. Analytikerin > Histolabor > Akh Linz > Krankenhausstr. 9 > 4020 Linz > +43(0)732/7806-6754 > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Jim > Manavis > Gesendet: Donnerstag, 28. Februar 2008 03:41 > An: Histonet > Betreff: [Histonet] hb crystals > > Dear Sir or Madam: > > > > I am a PhD student studying intracerebral haemorrhage in the rat and have > found numerous crystals contained within the haemorrhage, which I presume > are haemoglobin crystals, although it would be nice to demonstrate this. > Pearl's stain doesn't work, presumably because the iron is ferrous and bound > tightly, and Lillie's method with ferricyanide and HCL likewise doesn't > work, presumably because the iron is bound. Our standard DAB technique for > immunohistochemistry doesn't seem to react either. How might I best > determine whether or not the crystals are haemoglobin crystals? > > > > Yours Sincerely, > > > > Tim Kleinig > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From jhabecke <@t> fhcrc.org Thu Feb 28 18:27:54 2008 From: jhabecke <@t> fhcrc.org (Randolph-Habecker, Julie) Date: Thu Feb 28 18:28:03 2008 Subject: [Histonet] CCR5 antibody for FFPE tissue Message-ID: <040346FA7309BD439C327F97D4C4D69B01AEE466@ISIS.fhcrc.org> Folks, I am looking for an antibody to detect CCR5 in formalin-fixed paraffin-embedded human tissue. Does anyone have a recommendation of an antibody that works great? Thanks so much!! Julie Julie Randolph-Habecker, Ph.D. Staff Scientist - Director Experimental Histopathology Shared Resource Fred Hutchinson Cancer Research Center 1100 Fairview Ave, N. M5-A803 Seattle WA 98109-1024 206-667-6119 jhabecke@fhcrc.org From rchiovetti <@t> yahoo.com Thu Feb 28 19:08:52 2008 From: rchiovetti <@t> yahoo.com (Robert Chiovetti) Date: Thu Feb 28 19:08:56 2008 Subject: [Histonet] cryostat question Message-ID: <232692.83727.qm@web58913.mail.re1.yahoo.com> Emily, It's probably a small vacuum inlet that opens and closes under the antiroll plate, and it's likely adjustable so you can have a small amount of vacuum flowing while you're cutting to flatten out the sections under the antiroll plate. This has been an optional feature on the Microm/Richard-Allan/Thermo/Fisher/Whoevertheyaretoday cryostats for a couple of years. I don't know about the new Leica, but the Microm/etc. vacuum systems tend to clog up when they're used for waste removal (using the vacuum to remove all of the section debris that would normally collect in and around the microtome). Sooner or later the frozen sections reach an area in the vacuum tube where it's room temp, the OCT thaws, gets sticky and you have to go fishing with a long piece of wire to dig out the OCT gunk. The answer to this is of course to keep the waste reservoir and the in-line filter in the cold area of the cryostat so the OCT doesn't thaw. Maybe Leica has done this? If so, it would be a great improvement! My $2 worth... Bob Robert (Bob) Chiovetti, Ph.D. Southwest Precision Instruments www.swpinet.com Arizona's Microscopy Resource 132 North Elster Drive Tucson, AZ 85710-3212 Tel./Fax 520-546-4986 Member, Arizona Small Business Association (www.asba.com) ----- Original Message ---- From: Emily Sours To: histonet@lists.utsouthwestern.edu Sent: Thursday, February 28, 2008 3:09:11 PM Subject: [Histonet] cryostat question Any opinions on Leica's newest model, CM1950? I can't figure out how one could keep a vacuum in a cryostat that's being used. Emily -- fortune smiles on the brave and spits on the coward --aguirre, wrath of god _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Looking for last minute shopping deals? Find them fast with Yahoo! Search. http://tools.search.yahoo.com/newsearch/category.php?category=shopping From RSRICHMOND <@t> aol.com Thu Feb 28 20:21:07 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Thu Feb 28 20:21:14 2008 Subject: [Histonet] Re: Oil of Cajeput?? Message-ID: Oil of cajeput (the Oxford English Dictionary pronounces it CADGE-uh-putt - from a Malay word meaning 'white wood') is an oil distilled from any of several species of Melaleuca, so called tea-trees because the oil smells like green tea, to some people anyway. Tea-tree oil, a terpenoid like limonene, apparently has some antiseptic properties, and is widely used in cosmetics - I've got some tea tree oil shampoo in the bathroom, in fact. Hardly endangered, the Australian tea tree is the Tree that Ate Florida, where it's apparently taking over 50 acres a day. Clearly it's our environmental duty to clear in tea-tree oil! Histologists against the Tea Tree! I want a bumper sticker. Bob Richmond Samurai Pathologist Knoxville TN From BMolinari <@t> heart.thi.tmc.edu Fri Feb 29 05:40:49 2008 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Feb 29 05:40:47 2008 Subject: [Histonet] Oil of Cajeput In-Reply-To: References: <4D14F0FC9316DD41972D5F03C070908B017E61B7@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: Susan, Your King Arthur comment made me laugh this morning. Thanks for a laugh to start my Friday! Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston, TX 77030 832-355-6524 832-355-6812(fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of scoop@mail.nih.gov Sent: Thursday, February 28, 2008 2:52 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Oil of Cajeput I think it's the same as tea tree oil - you can get it at a health food store. I think it's used as a mosquito repellent and for skin or something. >I think King Arthur used the last bottle pulling Excalibur from the >stone... > > > >Sally Breeden, HT(ASCP) > >NM Dept. of Agriculture > >Veterinary Diagnostic Services > >PO Box 4700 > >Albuquerque, NM 87106 > >505-841-2576 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmjohnson34 <@t> hotmail.com Fri Feb 29 09:15:00 2008 From: jmjohnson34 <@t> hotmail.com (Jennifer Johnson) Date: Fri Feb 29 09:15:11 2008 Subject: [Histonet] Premiere microscope slides Message-ID: Amy, I sampled a case and hated them. My sales rep said they had been very well received and I was the only one complaining about them but my top gripes were: 1) They were too short and I couldn't fit as many sections on a slide. 2) The bottom corners are angled (or clipped) and when I put the slide on a staining tray, all of the reagent streamed off the corner. This also happened with mounting medium when I coverslipped them. 3) When I put them in a staining rack, it scraped the tissue off because they were shorter. 4) The frosted edge turns pink when stained with H & E which my Pathologist hated. 5) You cannot easily "float off a section" in the water bath if you are not happy with it. It sticks like glue. I had ten reasons but the others must not have been so aweful because I can't remember them. ;0 I would suggest getting a sample and seeing what you think. Jennifer _________________________________________________________________ Connect and share in new ways with Windows Live. http://www.windowslive.com/share.html?ocid=TXT_TAGHM_Wave2_sharelife_012008 From gu.lang <@t> gmx.at Fri Feb 29 09:46:08 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Feb 29 09:46:22 2008 Subject: AW: AW: [Histonet] hb crystals In-Reply-To: <47C7516E.5070705@shaw.ca> Message-ID: <000f01c87aea$340778e0$eeeea8c0@dielangs.at> I have to admit, that I have never seen it personally. But in my histobooks, there is allways the hint, that that could happen with tissue that was fixed in acid formalin (as staining-artefacts). Perhaps with PAS it forms a purple colour around the pigment? Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: Paul Bradbury [mailto:histology.bc@shaw.ca] Gesendet: Freitag, 29. Februar 2008 01:27 An: gu.lang@gmx.at; HistoNet Server Betreff: Re: AW: [Histonet] hb crystals I have never seen the PAS reaction give positive results with formalin pigment. Even if it did react, how would it be seen. The pigment is already dark brown-black, so colouring it red would have no visible effect. The same problem applies to any silver methods which might work ... it's already almost black, so making it a "darker black" would not be much help. The only definitive way to identify it is to use its birefringent properties. Paul Kamloops, Canada Gudrun Lang wrote: > If this crystals are formalin-pigment they have reducing capacitiy. > Therefore with silverimpregnation techniques like Masson this would render > black spots. Also the PAS stain would give positivity. > > Gudrun Lang > > Biomed. Analytikerin > Histolabor > Akh Linz > Krankenhausstr. 9 > 4020 Linz > +43(0)732/7806-6754 > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Jim > Manavis > Gesendet: Donnerstag, 28. Februar 2008 03:41 > An: Histonet > Betreff: [Histonet] hb crystals > > Dear Sir or Madam: > > > > I am a PhD student studying intracerebral haemorrhage in the rat and have > found numerous crystals contained within the haemorrhage, which I presume > are haemoglobin crystals, although it would be nice to demonstrate this. > Pearl's stain doesn't work, presumably because the iron is ferrous and bound > tightly, and Lillie's method with ferricyanide and HCL likewise doesn't > work, presumably because the iron is bound. Our standard DAB technique for > immunohistochemistry doesn't seem to react either. How might I best > determine whether or not the crystals are haemoglobin crystals? > > > > Yours Sincerely, > > > > Tim Kleinig > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From wilson_c <@t> ricerca.com Fri Feb 29 09:50:08 2008 From: wilson_c <@t> ricerca.com (Wilson, Carol) Date: Fri Feb 29 09:50:16 2008 Subject: [Histonet] Modified Davidson's Fixative Source Message-ID: <9D443EB9D0270143B5AAF190CB1A58A30679629A@dogwood.ricerca.com> Hi All, Anyone our there purchasing Modified Davidson's Fixative, and if so who is your vendor? Thanks in Advance, Carol Carol Wilson Team Leader - Histology Ricerca Biosciences, LLC From kenneth.metzger <@t> aruplab.com Fri Feb 29 09:59:38 2008 From: kenneth.metzger <@t> aruplab.com (Metzger, Kenneth) Date: Fri Feb 29 09:56:56 2008 Subject: [Histonet] Bone Marrow Issues Message-ID: We are seeing an artifact that causes loss of nuclear detail, particularly in erythroid cells, giving an appearance of being blown up with no nuclear detail. It is worse in decaled cores. This appears when we fix the cores and clot in formalin. We were using Z-5 and did not have this issue. My Pathologist wants to stick to formalin. None of our other tissue has this issue. Has anyone had this happen? Any help would be appreciated. Ken Ken Metzger HTL(ASCP) Histology Supervisor ARUP Laboratories 500 Chipeta way Salt Lake City, UT 84108 801.583.2787 ext 3101 - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From mcauliff <@t> umdnj.edu Fri Feb 29 09:25:13 2008 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Feb 29 09:57:18 2008 Subject: [Histonet] Re: RED COUNTERSTAINS In-Reply-To: References: Message-ID: <47C823D9.5040500@umdnj.edu> Hi Bob: I bought some Brazilliant recently and mixed it according to the directions provided. The results were dissipointing, red-orange nuclei, not very intense. The tissue was rabbit kidney fixed in formalin-alcohol-acetic. An alum hematoxylin on the same tissue looks fine. Geoff Robert Richmond wrote: > Diana McCaig asks about the nuclear counterstains nuclear fast red and > neutral red. > > Does anyone on this list have any experience with Anatech's > "Brazilliant", their trade name for alum brazilin, closely related to > alum hematoxylin, but red instead of purple? This looks to me like a > very logical red nuclear stain, and I'd certainly like to see it in > action if it were possible for me to obtain it (remember that hospital > pathology services are not usually permitted to order from small > companies like Anatech and the Davidson marking ink people). > > As everybody on this list ought to know, hematoxylin is a dye > extracted from the logwood tree (Haematoxylum campechianum), with an > aluminum mordant. (There is no satisfactory synthetic substitute.) > Brazilin is structurally very similar, but with an alum mordant it is > red rather than purple. Brazilin is extracted from the closely related > brazil woods, Caesalpinia echinata or C. sappan. > > One would expect this red dye to have the same staining specificity as > hematoxylin, and it should not wash out in aqueous mounting media. > > (I have no connection with Anatech.) > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From marktarango <@t> gmail.com Fri Feb 29 10:00:57 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Feb 29 10:01:02 2008 Subject: [Histonet] Bone Marrow Issues In-Reply-To: References: Message-ID: <5b6eb13e0802290800t617901eck765d1156ad642a0@mail.gmail.com> How long are they in formalin before going into decal? What kind of decal are you doing? On Fri, Feb 29, 2008 at 7:59 AM, Metzger, Kenneth < kenneth.metzger@aruplab.com> wrote: > We are seeing an artifact that causes loss of nuclear detail, particularly > in erythroid cells, giving an appearance of being blown up with no nuclear > detail. It is worse in decaled cores. This appears when we fix the cores and > clot in formalin. We were using Z-5 and did not have this issue. My > Pathologist wants to stick to formalin. None of our other tissue has this > issue. Has anyone had this happen? Any help would be appreciated. > > Ken > > > Ken Metzger HTL(ASCP) > Histology Supervisor > ARUP Laboratories > 500 Chipeta way > Salt Lake City, UT 84108 > 801.583.2787 ext 3101 > > > - ------------------------------------------------------------------ > The information transmitted by this e-mail and any included > attachments are from ARUP Laboratories and are intended only for the > recipient. The information contained in this message is confidential > and may constitute inside or non-public information under > international, federal, or state securities laws, or protected health > information and is intended only for the use of the recipient. > Unauthorized forwarding, printing, copying, distributing, or use of > such information is strictly prohibited and may be unlawful. If you > are not the intended recipient, please promptly delete this e-mail > and notify the sender of the delivery error or you may call ARUP > Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 > (800) 522-2787 ext. 2100 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From trathborne <@t> somerset-healthcare.com Fri Feb 29 10:01:18 2008 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Fri Feb 29 10:01:24 2008 Subject: [Histonet] Bone Marrow Issues In-Reply-To: Message-ID: We fix ours in zinc-formalin and do not have this problem. Toni -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Metzger, Kenneth Sent: Friday, February 29, 2008 11:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bone Marrow Issues We are seeing an artifact that causes loss of nuclear detail, particularly in erythroid cells, giving an appearance of being blown up with no nuclear detail. It is worse in decaled cores. This appears when we fix the cores and clot in formalin. We were using Z-5 and did not have this issue. My Pathologist wants to stick to formalin. None of our other tissue has this issue. Has anyone had this happen? Any help would be appreciated. Ken Ken Metzger HTL(ASCP) Histology Supervisor ARUP Laboratories 500 Chipeta way Salt Lake City, UT 84108 801.583.2787 ext 3101 - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. From fudo <@t> ufl.edu Fri Feb 29 10:03:54 2008 From: fudo <@t> ufl.edu (FU,DONGTAO) Date: Fri Feb 29 10:04:03 2008 Subject: [Histonet] frozen slides storage Message-ID: <1366601184.432261204301034872.JavaMail.osg@osgjas03.cns.ufl.edu> Hi, all I have a question about how to organize frozen slides in freezer? We have a lot of slide boxes inside the -80c freezer. It is very hard to find which one is which one when we start to do IHC staining several weeks later. We want to organize it and put all the information in the computer system. Right now I found the companies only sell racks for organizing blocks, not for slides(We usually use 100 slides box for one submitter). Does anyone have any experience on it? How do you organize your unstained slides? Thank you, Ann Dongtao Fu MD, Ph.D Lab Manager Dept. of Pathology Lab phone: 352-273-7752 Lab FAX: 352-273-7755 Lab address: D11-50 PO Box: 100275 1600 SW Archer Road University of Flodrida Gainesville, FL 32610 From rjbuesa <@t> yahoo.com Fri Feb 29 10:08:57 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 29 10:09:05 2008 Subject: [Histonet] Bone Marrow Issues In-Reply-To: Message-ID: <985244.25417.qm@web65706.mail.ac4.yahoo.com> Kenneth: BM sections, that are ussually sectioned thinner than others a per pathologists' request. are more prone to have this artifact ("bubble" or "empty" nuclei) cause when you DRY the sections in the oven that have NOT been totally drained. If there is some amount of water underneath the section when it goes into the dying oven, the nuclei contents gets that appearance. This artifact has nothing to do with the fixative, is a problem cause by incompletely dained sections. Ren? J. "Metzger, Kenneth" wrote: We are seeing an artifact that causes loss of nuclear detail, particularly in erythroid cells, giving an appearance of being blown up with no nuclear detail. It is worse in decaled cores. This appears when we fix the cores and clot in formalin. We were using Z-5 and did not have this issue. My Pathologist wants to stick to formalin. None of our other tissue has this issue. Has anyone had this happen? Any help would be appreciated. Ken Ken Metzger HTL(ASCP) Histology Supervisor ARUP Laboratories 500 Chipeta way Salt Lake City, UT 84108 801.583.2787 ext 3101 - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From rjbuesa <@t> yahoo.com Fri Feb 29 10:26:58 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 29 10:27:09 2008 Subject: [Histonet] frozen slides storage In-Reply-To: <1366601184.432261204301034872.JavaMail.osg@osgjas03.cns.ufl.edu> Message-ID: <27667.8281.qm@web65709.mail.ac4.yahoo.com> I used plastic boxes for 25 slides each. Ren? J. "FU,DONGTAO" wrote: Hi, all I have a question about how to organize frozen slides in freezer? We have a lot of slide boxes inside the -80c freezer. It is very hard to find which one is which one when we start to do IHC staining several weeks later. We want to organize it and put all the information in the computer system. Right now I found the companies only sell racks for organizing blocks, not for slides(We usually use 100 slides box for one submitter). Does anyone have any experience on it? How do you organize your unstained slides? Thank you, Ann Dongtao Fu MD, Ph.D Lab Manager Dept. of Pathology Lab phone: 352-273-7752 Lab FAX: 352-273-7755 Lab address: D11-50 PO Box: 100275 1600 SW Archer Road University of Flodrida Gainesville, FL 32610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. From rjbuesa <@t> yahoo.com Fri Feb 29 10:27:28 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Feb 29 10:27:37 2008 Subject: [Histonet] frozen slides storage In-Reply-To: <1366601184.432261204301034872.JavaMail.osg@osgjas03.cns.ufl.edu> Message-ID: <802058.54303.qm@web65714.mail.ac4.yahoo.com> I used plastic boxes for 25 slides each. Ren? J. "FU,DONGTAO" wrote: --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. From SHargrove <@t> urhcs.org Fri Feb 29 10:49:11 2008 From: SHargrove <@t> urhcs.org (SHargrove@urhcs.org) Date: Fri Feb 29 10:49:45 2008 Subject: [Histonet] Susie Hargrove is out of the office. Message-ID: I will be out of the office starting 02/29/2008 and will not return until 03/05/2008. I will respond to your message when I return. If immediate assistance is needed please call 3198. From michael.owen <@t> fda.hhs.gov Fri Feb 29 10:50:41 2008 From: michael.owen <@t> fda.hhs.gov (Owen, Michael P) Date: Fri Feb 29 10:50:50 2008 Subject: [Histonet] M'Fadyean Staining of Bacillus anthracis Message-ID: <449E51C6DA0AD840B44F57C7A6EB07BF0422042E@FMD3VS022.fda.gov> Dear List Members, Do any of you still perform M'Fadyean (polychrome methylene blue) staining to visualize the poly-D-glutamic capsule of Bacillus anthracis? Did you encounter problems with the procedure? I am very interested in your experiences with the method including limitations encountered. You can contact me off the list to give responses. Thanks in advance. Michael P. Owen, Regulatory Microbiologist U.S. FDA Pacific Regional Lab Northwest 22201 23rd Drive SE Bothell, WA 98021-4421 Phone: 425-483-4865 E-Mail: michael.owen@fda.hhs.gov From sbreeden <@t> nmda.nmsu.edu Fri Feb 29 10:54:21 2008 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Feb 29 10:54:25 2008 Subject: [Histonet] Histologists for Cajeput Oil! Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E61BB@nmdamailsvr.nmda.ad.nmsu.edu> "Histologists for Cajeput Oil"! There's your bumper sticker. And if that doesn't completely befuddle 95% of the population by using two odd words in one bumper sticker, I don't know what will! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From Renwen.Zhang <@t> stryker.com Fri Feb 29 11:01:06 2008 From: Renwen.Zhang <@t> stryker.com (Zhang, Renwen) Date: Fri Feb 29 11:01:13 2008 Subject: [Histonet] Sectioning of MMA embedded block Message-ID: We have some MMA embedded tissue/material blocks. I am looking for a lab which can make micro sections from the block. The material in the block is as hard as bone. From detmar <@t> mshri.on.ca Fri Feb 29 11:38:44 2008 From: detmar <@t> mshri.on.ca (Jacqui Detmar) Date: Fri Feb 29 11:39:16 2008 Subject: [Histonet] Re: RED COUNTERSTAINS In-Reply-To: <47C823D9.5040500@umdnj.edu> References: , <47C823D9.5040500@umdnj.edu> Message-ID: <38F7D55F-A6C0-44AD-9DA1-9A2685448BFC@mimectl> Hi all. I also bought some brazilin powder (from Anatech) and mixed it according to directions, and the initial results were disappointing. I then prepared an alum brazilin solution (recommended by someone on Histonet, I think, but I'm at a conference right now and don't have access to my e-files! ), which is pretty much the same way you would make alum hematoxylin, except you substitute brazilin for hematoxylin. I remember having to use slight heating to get some of the powder into solution, and even then, not all of the brazilin went into solution (it falls to the bottom...I guess you could filter it, but I've never bothered). I find that I get the best results if I stain my slides for 20-30 minutes in the alum brazilin, followed by tap-water washes and a final "blue-ing" in Scott's blueing solution for 30 seconds. The result is a nice, raspberry-coloured stain. The recipe for the brazilin solution is in the sheet given by the manufacturer. I love this stain and I no longer use nuclear fast red. The only time I've had trouble with it is with the von Kossa stain. You *cannot* use brazilin after von Kossa...the stain goes away for some reason...you need to use methyl green or nuclear fast red. I think that's about it! If you have any questions, please feel free to ask! Jacqui Jacqui Detmar, Post-doctoral Fellow Samuel Lunenfeld Research Institute, room 876 Mount Sinai Hospital 600 University Avenue, Toronto, ON, Canada M5G 1X5 Tel: 416-586-4800 x2451/x2290 Fax: 416-586-8588 email: detmar@mshri.on.ca From: Geoff McAuliffe Sent: Fri 2/29/2008 10:25 AM To: Robert Richmond Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: RED COUNTERSTAINS Hi Bob: I bought some Brazilliant recently and mixed it according to the directions provided. The results were dissipointing, red-orange nuclei, not very intense. The tissue was rabbit kidney fixed in formalin-alcohol-acetic. An alum hematoxylin on the same tissue looks fine. Geoff Robert Richmond wrote: > Diana McCaig asks about the nuclear counterstains nuclear fast red and > neutral red. > > Does anyone on this list have any experience with Anatech's > "Brazilliant", their trade name for alum brazilin, closely related to > alum hematoxylin, but red instead of purple? This looks to me like a > very logical red nuclear stain, and I'd certainly like to see it in > action if it were possible for me to obtain it (remember that hospital > pathology services are not usually permitted to order from small > companies like Anatech and the Davidson marking ink people). > > As everybody on this list ought to know, hematoxylin is a dye > extracted from the logwood tree (Haematoxylum campechianum), with an > aluminum mordant. (There is no satisfactory synthetic substitute.) > Brazilin is structurally very similar, but with an alum mordant it is > red rather than purple. Brazilin is extracted from the closely related > brazil woods, Caesalpinia echinata or C. sappan. > > One would expect this red dye to have the same staining specificity as > hematoxylin, and it should not wash out in aqueous mounting media. > > (I have no connection with Anatech.) > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rmweber113 <@t> comcast.net Fri Feb 29 11:41:52 2008 From: rmweber113 <@t> comcast.net (rmweber113@comcast.net) Date: Fri Feb 29 11:42:05 2008 Subject: FW: [Histonet] Histology Supervisor Position NY Message-ID: <022920081741.23695.47C843E000002D0500005C8F2215586394CCCECE9D0A0D0A99039D@comcast.net> -------------- Forwarded Message: -------------- From: rmweber113@comcast.net To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology Supervisor Position NY Date: Wed, 27 Feb 2008 16:38:58 +0000 I have a position for a Histology Supervisor available at an established GI group located in New City, NY, Rockland County. Candidate should have the knowledge to set up and maintain a state of the art facility. Knowledge of grossing, processing and routine histology of gastric biopsies required. $30.00/hr plus benefits. Qualified candidates can email me at rmweber113@comcast.net or call me at 732 814-1170 $500.00 for a successful referals committed for at least 90 days. Marilynn Weber H.T. (ASCP) QIHC Twincrest _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmiller <@t> physlab.com Fri Feb 29 11:59:48 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Fri Feb 29 11:59:12 2008 Subject: [Histonet] rapid freeze containers Message-ID: <000001c87afc$df974840$3d02a8c0@plab.local> How is everyone disposing of their rapid freeze aerosol cans when empty? It is considered hazardous waste here in Omaha, not bio hazard but hazardous in the terms that the can is under pressure. We can purchase a can puncturer for 1000.00 but that seems steep. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From Ronald.Houston <@t> nationwidechildrens.org Fri Feb 29 12:12:22 2008 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri Feb 29 12:13:13 2008 Subject: [Histonet] Re: RED COUNTERSTAINS In-Reply-To: <38F7D55F-A6C0-44AD-9DA1-9A2685448BFC@mimectl> Message-ID: <979FF5962E234F45B06CF0DB7C1AABB215A6F237@chi2k3ms01.columbuschildrens.net> Can't think why you would want to wait 20-30 minutes for a nuclear counterstain Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 Ronald.Houston@NationwideChildrens.org Columbus Children's Hospital is now Nationwide Children's Hospital www.NationwideChildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jacqui Detmar Sent: Friday, February 29, 2008 12:39 PM To: Geoff McAuliffe; Robert Richmond Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: RED COUNTERSTAINS Hi all. I also bought some brazilin powder (from Anatech) and mixed it according to directions, and the initial results were disappointing. I then prepared an alum brazilin solution (recommended by someone on Histonet, I think, but I'm at a conference right now and don't have access to my e-files! ), which is pretty much the same way you would make alum hematoxylin, except you substitute brazilin for hematoxylin. I remember having to use slight heating to get some of the powder into solution, and even then, not all of the brazilin went into solution (it falls to the bottom...I guess you could filter it, but I've never bothered). I find that I get the best results if I stain my slides for 20-30 minutes in the alum brazilin, followed by tap-water washes and a final "blue-ing" in Scott's blueing solution for 30 seconds. The result is a nice, raspberry-coloured stain. The recipe for the brazilin solution is in the sheet given by the manufacturer. I love this stain and I no longer use nuclear fast red. The only time I've had trouble with it is with the von Kossa stain. You *cannot* use brazilin after von Kossa...the stain goes away for some reason...you need to use methyl green or nuclear fast red. I think that's about it! If you have any questions, please feel free to ask! Jacqui Jacqui Detmar, Post-doctoral Fellow Samuel Lunenfeld Research Institute, room 876 Mount Sinai Hospital 600 University Avenue, Toronto, ON, Canada M5G 1X5 Tel: 416-586-4800 x2451/x2290 Fax: 416-586-8588 email: detmar@mshri.on.ca From: Geoff McAuliffe Sent: Fri 2/29/2008 10:25 AM To: Robert Richmond Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: RED COUNTERSTAINS Hi Bob: I bought some Brazilliant recently and mixed it according to the directions provided. The results were dissipointing, red-orange nuclei, not very intense. The tissue was rabbit kidney fixed in formalin-alcohol-acetic. An alum hematoxylin on the same tissue looks fine. Geoff Robert Richmond wrote: > Diana McCaig asks about the nuclear counterstains nuclear fast red and > neutral red. > > Does anyone on this list have any experience with Anatech's > "Brazilliant", their trade name for alum brazilin, closely related to > alum hematoxylin, but red instead of purple? This looks to me like a > very logical red nuclear stain, and I'd certainly like to see it in > action if it were possible for me to obtain it (remember that hospital > pathology services are not usually permitted to order from small > companies like Anatech and the Davidson marking ink people). > > As everybody on this list ought to know, hematoxylin is a dye > extracted from the logwood tree (Haematoxylum campechianum), with an > aluminum mordant. (There is no satisfactory synthetic substitute.) > Brazilin is structurally very similar, but with an alum mordant it is > red rather than purple. Brazilin is extracted from the closely related > brazil woods, Caesalpinia echinata or C. sappan. > > One would expect this red dye to have the same staining specificity as > hematoxylin, and it should not wash out in aqueous mounting media. > > (I have no connection with Anatech.) > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. From PMonfils <@t> Lifespan.org Fri Feb 29 12:39:19 2008 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Feb 29 12:39:28 2008 Subject: [Histonet] rapid freeze containers In-Reply-To: <000001c87afc$df974840$3d02a8c0@plab.local> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273D29@LSRIEXCH1.lsmaster.lifespan.org> Does this regulation apply only to institutions? What do you do with household aerosol cans when empty? From JMacDonald <@t> mtsac.edu Fri Feb 29 12:45:35 2008 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Feb 29 12:45:38 2008 Subject: AW: [Histonet] hb crystals In-Reply-To: <47C7516E.5070705@shaw.ca> Message-ID: Formalin pigment can be confirmed by removing it. You can use alcoholic picric acid or alkaline alcohol. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu Paul Bradbury Sent by: histonet-bounces@lists.utsouthwestern.edu 02/28/2008 04:27 PM To gu.lang@gmx.at, HistoNet Server cc Subject Re: AW: [Histonet] hb crystals I have never seen the PAS reaction give positive results with formalin pigment. Even if it did react, how would it be seen. The pigment is already dark brown-black, so colouring it red would have no visible effect. The same problem applies to any silver methods which might work ... it's already almost black, so making it a "darker black" would not be much help. The only definitive way to identify it is to use its birefringent properties. Paul Kamloops, Canada Gudrun Lang wrote: > If this crystals are formalin-pigment they have reducing capacitiy. > Therefore with silverimpregnation techniques like Masson this would render > black spots. Also the PAS stain would give positivity. > > Gudrun Lang > > Biomed. Analytikerin > Histolabor > Akh Linz > Krankenhausstr. 9 > 4020 Linz > +43(0)732/7806-6754 > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Jim > Manavis > Gesendet: Donnerstag, 28. Februar 2008 03:41 > An: Histonet > Betreff: [Histonet] hb crystals > > Dear Sir or Madam: > > > > I am a PhD student studying intracerebral haemorrhage in the rat and have > found numerous crystals contained within the haemorrhage, which I presume > are haemoglobin crystals, although it would be nice to demonstrate this. > Pearl's stain doesn't work, presumably because the iron is ferrous and bound > tightly, and Lillie's method with ferricyanide and HCL likewise doesn't > work, presumably because the iron is bound. Our standard DAB technique for > immunohistochemistry doesn't seem to react either. How might I best > determine whether or not the crystals are haemoglobin crystals? > > > > Yours Sincerely, > > > > Tim Kleinig > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Fri Feb 29 12:57:21 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Feb 29 12:54:37 2008 Subject: [Histonet] Histologists for Cajeput Oil! In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E61BB@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B017E61BB@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <1F937FB30BDB7C4A9F39F83FEA8D379F9F528F@bruexchange1.digestivespecialists.com> Good idea Sally! You print them and I know a lot of us will display them. L Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, February 29, 2008 11:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histologists for Cajeput Oil! "Histologists for Cajeput Oil"! There's your bumper sticker. And if that doesn't completely befuddle 95% of the population by using two odd words in one bumper sticker, I don't know what will! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> yahoo.com Fri Feb 29 13:54:32 2008 From: akemiat3377 <@t> yahoo.com (Akemi Allison-Tacha) Date: Fri Feb 29 13:54:39 2008 Subject: [Histonet] CAP on Monday Message-ID: <682804.4723.qm@web31306.mail.mud.yahoo.com> Hi All, It's Friday, sunny and life is beautiful! As for the CAP inspection on Monday, the inspectors found virtually no deficiencies, and had the most laudatory of comments about the laboratory and its personnel. My department had absolutly no deficiencies. Overall, it was an wonderful confirmation of the level of excellence we have attained and maintained here at PhenoPath, and a testament to the efforts of each and every one of our staff that helped ensure this. Regards, Akemi Akemi Allison-Tacha, BS, HT(ASCP)HTL Client Services Manager PhenoPath laboratories 551 North 34th Street, Suite 100 Seattle, WA 98103-8675 Work: (206) 374-9000 Cell: (425) 941-4287 E-Mail: akemiat3377@yahoo.com From gentras <@t> vetmed.auburn.edu Fri Feb 29 14:12:51 2008 From: gentras <@t> vetmed.auburn.edu (Atoska Gentry) Date: Fri Feb 29 14:13:01 2008 Subject: [Histonet] cryosections Message-ID: <47C86743.5060505@vetmed.auburn.edu> hello, does anyone know the cause/remedy for excessive air bubbles & wrinkles in frozen cat brain sections that have been fixed in 4% PFA and cryoprotected? Thanks, Atoska -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu From SCOTT.TURNER <@t> SPCORP.COM Fri Feb 29 15:00:19 2008 From: SCOTT.TURNER <@t> SPCORP.COM (Turner, Scott) Date: Fri Feb 29 15:00:30 2008 Subject: [Histonet] frozen slides storage In-Reply-To: <1366601184.432261204301034872.JavaMail.osg@osgjas03.cns.ufl.edu> Message-ID: <9A919A5D70313A4D9C56A025710874080313D69C@kenmsg40.us.schp.com> Research Products International makes freezer racks that hold the black 25 slide boxes, as well as 100 slide boxes. We use the 25 slide box racks and they are quite good for organizing slides in the freezer. Check them out: http://www.rpicorp.com/products/prod_info.html?products_front_id=1159&ca tid=5&cat_id=77 Scott Turner Schering-Plough Biopharma 901 California Ave Palo Alto, CA 94304 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of FU,DONGTAO Sent: Friday, February 29, 2008 08:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] frozen slides storage Hi, all I have a question about how to organize frozen slides in freezer? We have a lot of slide boxes inside the -80c freezer. It is very hard to find which one is which one when we start to do IHC staining several weeks later. We want to organize it and put all the information in the computer system. Right now I found the companies only sell racks for organizing blocks, not for slides(We usually use 100 slides box for one submitter). Does anyone have any experience on it? How do you organize your unstained slides? Thank you, Ann Dongtao Fu MD, Ph.D Lab Manager Dept. of Pathology Lab phone: 352-273-7752 Lab FAX: 352-273-7755 Lab address: D11-50 PO Box: 100275 1600 SW Archer Road University of Flodrida Gainesville, FL 32610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From RSRICHMOND <@t> aol.com Fri Feb 29 15:02:37 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Fri Feb 29 15:02:48 2008 Subject: [Histonet] Re: RED COUNTERSTAINS Message-ID: Jacqui Detmar and Geoff McAuliffe both report difficulty in preparing Anatech's Brazilliant brazilin-alum from their dry preparation. Maybe it would be better to try the liquid Brazilliant preparation initially. Wish I could get my hands on it. In none of the three pathology services I'm working on at the moment is the pathology service allowed to order from any alternative vendor, nor does a histotech ever look at a slide. Thinking, time to retire. Bob Richmond Samurai Pathologist Knoxville TN From Carmen.Wynn <@t> us.astellas.com Fri Feb 29 15:28:14 2008 From: Carmen.Wynn <@t> us.astellas.com (Wynn, Carmen) Date: Fri Feb 29 15:29:08 2008 Subject: [Histonet] frozen slides storage In-Reply-To: <20080229180843.9628D918086@mail185-dub.bigfish.com> References: <20080229180843.9628D918086@mail185-dub.bigfish.com> Message-ID: Hello Ann, I have experienced that problem as well. I work in a research lab that focuses on mouse and rat tissue so I obtained colored micro slide boxes that I label on both the face and hinge side. I store my boxes with the hinge side facing the door so that I can maximize space. In my case I used green color frost plus microscope slides and green boxes for mouse tissue and red color frost microscope plus slides and red boxes for rat tissue. It works out nice for me because when the frost builds up in the freezer at least you can narrow down the tissue type and area just by looking for colors. I keep mouse tissue on one shelf and rat on another. The tissue that I am currently working with is always kept in the front so that I can reach it quickly without setting off the freezer alarm and letting to much heat in. It worked so well for me that I store all my paraffin samples the same way. I have seen at least 2 other colors of boxes available....Blue and Yellow. The boxes and slides are available thru Fisher or VWR. However, the cheapest micro slide box vendor that I have used is Lab Storage Systems (I paid $6.45/box). I also have gotten great deals on Color Frost and Color frost Plus slides from Lab Source (in IL). I hope this helps otherwise you should in vest in a good set of really thick mittens for those cold hands. Carmen Wynn, M.S., Senior Scientist Astellas Research Institute of America, LLC. (ARIA) Illinois Science and Technology Park 8045 Lamon Ave Skokie, IL 60077 Direct: 847-933-7419 Main: 847-933-7400 Fax: 847-933-7401 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, February 29, 2008 12:09 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 51, Issue 44 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Bone Marrow Issues (Rene J Buesa) 2. Re: frozen slides storage (Rene J Buesa) 3. Re: frozen slides storage (Rene J Buesa) 4. Susie Hargrove is out of the office. (SHargrove@urhcs.org) 5. M'Fadyean Staining of Bacillus anthracis (Owen, Michael P) 6. Histologists for Cajeput Oil! (Breeden, Sara) 7. Sectioning of MMA embedded block (Zhang, Renwen) 8. RE: Re: RED COUNTERSTAINS (Jacqui Detmar) 9. FW: [Histonet] Histology Supervisor Position NY (rmweber113@comcast.net) 10. rapid freeze containers (Cheri Miller) ---------------------------------------------------------------------- Message: 1 Date: Fri, 29 Feb 2008 08:08:57 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Bone Marrow Issues To: "Metzger, Kenneth" , histonet@lists.utsouthwestern.edu Message-ID: <985244.25417.qm@web65706.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Kenneth: BM sections, that are ussually sectioned thinner than others a per pathologists' request. are more prone to have this artifact ("bubble" or "empty" nuclei) cause when you DRY the sections in the oven that have NOT been totally drained. If there is some amount of water underneath the section when it goes into the dying oven, the nuclei contents gets that appearance. This artifact has nothing to do with the fixative, is a problem cause by incompletely dained sections. Ren J. "Metzger, Kenneth" wrote: We are seeing an artifact that causes loss of nuclear detail, particularly in erythroid cells, giving an appearance of being blown up with no nuclear detail. It is worse in decaled cores. This appears when we fix the cores and clot in formalin. We were using Z-5 and did not have this issue. My Pathologist wants to stick to formalin. None of our other tissue has this issue. Has anyone had this happen? Any help would be appreciated. Ken Ken Metzger HTL(ASCP) Histology Supervisor ARUP Laboratories 500 Chipeta way Salt Lake City, UT 84108 801.583.2787 ext 3101 - ------------------------------------------------------------------ The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. ------------------------------ Message: 2 Date: Fri, 29 Feb 2008 08:26:58 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] frozen slides storage To: "FU,DONGTAO" , histonet@lists.utsouthwestern.edu Message-ID: <27667.8281.qm@web65709.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I used plastic boxes for 25 slides each. Ren J. "FU,DONGTAO" wrote: Hi, all I have a question about how to organize frozen slides in freezer? We have a lot of slide boxes inside the -80c freezer. It is very hard to find which one is which one when we start to do IHC staining several weeks later. We want to organize it and put all the information in the computer system. Right now I found the companies only sell racks for organizing blocks, not for slides(We usually use 100 slides box for one submitter). Does anyone have any experience on it? How do you organize your unstained slides? Thank you, Ann Dongtao Fu MD, Ph.D Lab Manager Dept. of Pathology Lab phone: 352-273-7752 Lab FAX: 352-273-7755 Lab address: D11-50 PO Box: 100275 1600 SW Archer Road University of Flodrida Gainesville, FL 32610 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Never miss a thing. Make Yahoo your homepage. ------------------------------ Message: 3 Date: Fri, 29 Feb 2008 08:27:28 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] frozen slides storage To: "FU,DONGTAO" , histonet@lists.utsouthwestern.edu Message-ID: <802058.54303.qm@web65714.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I used plastic boxes for 25 slides each. Ren J. "FU,DONGTAO" wrote: --------------------------------- Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now. ------------------------------ Message: 4 Date: Fri, 29 Feb 2008 10:49:11 -0600 From: SHargrove@urhcs.org Subject: [Histonet] Susie Hargrove is out of the office. To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 02/29/2008 and will not return until 03/05/2008. I will respond to your message when I return. If immediate assistance is needed please call 3198. ------------------------------ Message: 5 Date: Fri, 29 Feb 2008 11:50:41 -0500 From: "Owen, Michael P" Subject: [Histonet] M'Fadyean Staining of Bacillus anthracis To: "Histonet" Message-ID: <449E51C6DA0AD840B44F57C7A6EB07BF0422042E@FMD3VS022.fda.gov> Content-Type: text/plain; charset="us-ascii" Dear List Members, Do any of you still perform M'Fadyean (polychrome methylene blue) staining to visualize the poly-D-glutamic capsule of Bacillus anthracis? Did you encounter problems with the procedure? I am very interested in your experiences with the method including limitations encountered. You can contact me off the list to give responses. Thanks in advance. Michael P. Owen, Regulatory Microbiologist U.S. FDA Pacific Regional Lab Northwest 22201 23rd Drive SE Bothell, WA 98021-4421 Phone: 425-483-4865 E-Mail: michael.owen@fda.hhs.gov ------------------------------ Message: 6 Date: Fri, 29 Feb 2008 09:54:21 -0700 From: "Breeden, Sara" Subject: [Histonet] Histologists for Cajeput Oil! To: Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E61BB@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" "Histologists for Cajeput Oil"! There's your bumper sticker. And if that doesn't completely befuddle 95% of the population by using two odd words in one bumper sticker, I don't know what will! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ------------------------------ Message: 7 Date: Fri, 29 Feb 2008 12:01:06 -0500 From: "Zhang, Renwen" Subject: [Histonet] Sectioning of MMA embedded block To: Message-ID: Content-Type: text/plain; charset="us-ascii" We have some MMA embedded tissue/material blocks. I am looking for a lab which can make micro sections from the block. The material in the block is as hard as bone. ------------------------------ Message: 8 Date: Fri, 29 Feb 2008 12:38:44 -0500 From: Jacqui Detmar Subject: RE: [Histonet] Re: RED COUNTERSTAINS To: Geoff McAuliffe , Robert Richmond Cc: histonet@lists.utsouthwestern.edu Message-ID: <38F7D55F-A6C0-44AD-9DA1-9A2685448BFC@mimectl> Content-Type: text/plain; charset="iso-8859-1"; format=flowed Hi all. I also bought some brazilin powder (from Anatech) and mixed it according to directions, and the initial results were disappointing. I then prepared an alum brazilin solution (recommended by someone on Histonet, I think, but I'm at a conference right now and don't have access to my e-files! ), which is pretty much the same way you would make alum hematoxylin, except you substitute brazilin for hematoxylin. I remember having to use slight heating to get some of the powder into solution, and even then, not all of the brazilin went into solution (it falls to the bottom...I guess you could filter it, but I've never bothered). I find that I get the best results if I stain my slides for 20-30 minutes in the alum brazilin, followed by tap-water washes and a final "blue-ing" in Scott's blueing solution for 30 seconds. The result is a nice, raspberry-coloured stain. The recipe for the brazilin solution is in the sheet given by the manufacturer. I love this stain and I no longer use nuclear fast red. The only time I've had trouble with it is with the von Kossa stain. You *cannot* use brazilin after von Kossa...the stain goes away for some reason...you need to use methyl green or nuclear fast red. I think that's about it! If you have any questions, please feel free to ask! Jacqui Jacqui Detmar, Post-doctoral Fellow Samuel Lunenfeld Research Institute, room 876 Mount Sinai Hospital 600 University Avenue, Toronto, ON, Canada M5G 1X5 Tel: 416-586-4800 x2451/x2290 Fax: 416-586-8588 email: detmar@mshri.on.ca From: Geoff McAuliffe Sent: Fri 2/29/2008 10:25 AM To: Robert Richmond Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: RED COUNTERSTAINS Hi Bob: I bought some Brazilliant recently and mixed it according to the directions provided. The results were dissipointing, red-orange nuclei, not very intense. The tissue was rabbit kidney fixed in formalin-alcohol-acetic. An alum hematoxylin on the same tissue looks fine. Geoff Robert Richmond wrote: > Diana McCaig asks about the nuclear counterstains nuclear fast red and > neutral red. > > Does anyone on this list have any experience with Anatech's > "Brazilliant", their trade name for alum brazilin, closely related to > alum hematoxylin, but red instead of purple? This looks to me like a > very logical red nuclear stain, and I'd certainly like to see it in > action if it were possible for me to obtain it (remember that hospital > pathology services are not usually permitted to order from small > companies like Anatech and the Davidson marking ink people). > > As everybody on this list ought to know, hematoxylin is a dye > extracted from the logwood tree (Haematoxylum campechianum), with an > aluminum mordant. (There is no satisfactory synthetic substitute.) > Brazilin is structurally very similar, but with an alum mordant it is > red rather than purple. Brazilin is extracted from the closely related > brazil woods, Caesalpinia echinata or C. sappan. > > One would expect this red dye to have the same staining specificity as > hematoxylin, and it should not wash out in aqueous mounting media. > > (I have no connection with Anatech.) > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Fri, 29 Feb 2008 17:41:52 +0000 From: rmweber113@comcast.net Subject: FW: [Histonet] Histology Supervisor Position NY To: histonet@lists.utsouthwestern.edu Message-ID: <022920081741.23695.47C843E000002D0500005C8F2215586394CCCECE9D0A0D0A9903 9D@comcast.net> Content-Type: text/plain -------------- Forwarded Message: -------------- From: rmweber113@comcast.net To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology Supervisor Position NY Date: Wed, 27 Feb 2008 16:38:58 +0000 I have a position for a Histology Supervisor available at an established GI group located in New City, NY, Rockland County. Candidate should have the knowledge to set up and maintain a state of the art facility. Knowledge of grossing, processing and routine histology of gastric biopsies required. $30.00/hr plus benefits. Qualified candidates can email me at rmweber113@comcast.net or call me at 732 814-1170 $500.00 for a successful referals committed for at least 90 days. Marilynn Weber H.T. (ASCP) QIHC Twincrest _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Fri, 29 Feb 2008 11:59:48 -0600 From: "Cheri Miller" Subject: [Histonet] rapid freeze containers To: Message-ID: <000001c87afc$df974840$3d02a8c0@plab.local> Content-Type: text/plain; charset="us-ascii" How is everyone disposing of their rapid freeze aerosol cans when empty? It is considered hazardous waste here in Omaha, not bio hazard but hazardous in the terms that the can is under pressure. We can purchase a can puncturer for 1000.00 but that seems steep. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 51, Issue 44 **************************************** From cad <@t> Stowers-Institute.org Fri Feb 29 16:52:06 2008 From: cad <@t> Stowers-Institute.org (Dickey, Coral) Date: Fri Feb 29 16:52:31 2008 Subject: [Histonet] Zebrafish histology Message-ID: Thanks to all who replied to my inquiry about scales. I am still looking for a way to avoid mechanical debridement of the scales to allow best visualization of a whole mount stained skeleton. Have a great weekend, Coral Dickey Histology Specialist I Stowers Institute for Medical Research 1000 E. 50th Street Kansas City, Missouri 64110 Phone: 816-926-4305 e-mail: cad@stowers-institute.org From detmar <@t> mshri.on.ca Fri Feb 29 18:01:33 2008 From: detmar <@t> mshri.on.ca (Jacqui Detmar) Date: Fri Feb 29 18:06:47 2008 Subject: [Histonet] Re: RED COUNTERSTAINS References: <979FF5962E234F45B06CF0DB7C1AABB215A6F237@chi2k3ms01.columbuschildrens.net> Message-ID: Actually, I tend to run about 5 experiments at the same time so if I have to leave something for 30 minutes, I usually welcome the reprieve . Also, I was just getting such inconsistent results with nuclear fast red and it was irritating me . Having worked previously in Biotech and working currently in academia, I've discovered that what you won't tolerate in one, you will in the other and vice versa. Jacqui Jacqui Detmar, Post-doctoral Fellow Samuel Lunenfeld Research Institute, room 876 Mount Sinai Hospital 600 University Avenue, Toronto, ON, Canada M5G 1X5 Tel: 416-586-4800 x2451/x2290 Fax: 416-586-8588 email: detmar@mshri.on.ca ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Houston, Ronald Sent: Fri 2/29/2008 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: RED COUNTERSTAINS Can't think why you would want to wait 20-30 minutes for a nuclear counterstain Ronnie Houston, MS, HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital 700 Children's Drive Columbus, OH 43205 (614) 722 5465 Ronald.Houston@NationwideChildrens.org Columbus Children's Hospital is now Nationwide Children's Hospital www.NationwideChildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jacqui Detmar Sent: Friday, February 29, 2008 12:39 PM To: Geoff McAuliffe; Robert Richmond Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: RED COUNTERSTAINS Hi all. I also bought some brazilin powder (from Anatech) and mixed it according to directions, and the initial results were disappointing. I then prepared an alum brazilin solution (recommended by someone on Histonet, I think, but I'm at a conference right now and don't have access to my e-files! ), which is pretty much the same way you would make alum hematoxylin, except you substitute brazilin for hematoxylin. I remember having to use slight heating to get some of the powder into solution, and even then, not all of the brazilin went into solution (it falls to the bottom...I guess you could filter it, but I've never bothered). I find that I get the best results if I stain my slides for 20-30 minutes in the alum brazilin, followed by tap-water washes and a final "blue-ing" in Scott's blueing solution for 30 seconds. The result is a nice, raspberry-coloured stain. The recipe for the brazilin solution is in the sheet given by the manufacturer. I love this stain and I no longer use nuclear fast red. The only time I've had trouble with it is with the von Kossa stain. You *cannot* use brazilin after von Kossa...the stain goes away for some reason...you need to use methyl green or nuclear fast red. I think that's about it! If you have any questions, please feel free to ask! Jacqui Jacqui Detmar, Post-doctoral Fellow Samuel Lunenfeld Research Institute, room 876 Mount Sinai Hospital 600 University Avenue, Toronto, ON, Canada M5G 1X5 Tel: 416-586-4800 x2451/x2290 Fax: 416-586-8588 email: detmar@mshri.on.ca From: Geoff McAuliffe Sent: Fri 2/29/2008 10:25 AM To: Robert Richmond Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: RED COUNTERSTAINS Hi Bob: I bought some Brazilliant recently and mixed it according to the directions provided. The results were dissipointing, red-orange nuclei, not very intense. The tissue was rabbit kidney fixed in formalin-alcohol-acetic. An alum hematoxylin on the same tissue looks fine. Geoff Robert Richmond wrote: > Diana McCaig asks about the nuclear counterstains nuclear fast red and > neutral red. > > Does anyone on this list have any experience with Anatech's > "Brazilliant", their trade name for alum brazilin, closely related to > alum hematoxylin, but red instead of purple? This looks to me like a > very logical red nuclear stain, and I'd certainly like to see it in > action if it were possible for me to obtain it (remember that hospital > pathology services are not usually permitted to order from small > companies like Anatech and the Davidson marking ink people). > > As everybody on this list ought to know, hematoxylin is a dye > extracted from the logwood tree (Haematoxylum campechianum), with an > aluminum mordant. (There is no satisfactory synthetic substitute.) > Brazilin is structurally very similar, but with an alum mordant it is > red rather than purple. Brazilin is extracted from the closely related > brazil woods, Caesalpinia echinata or C. sappan. > > One would expect this red dye to have the same staining specificity as > hematoxylin, and it should not wash out in aqueous mounting media. > > (I have no connection with Anatech.) > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. 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