From ROrr <@t> enh.org Fri Aug 1 07:03:26 2008 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Fri Aug 1 07:03:34 2008 Subject: [Histonet] CCAR-1 antibody Message-ID: Hello Everyone, I am searching for anyone who could send me any part of their supply of the CCAR-1 antibody, (rabbit) part number HPA007856- Sigma- Aldrich I would need enough for about 40 cases, so if you already have a "working " dilution set up, I would be grateful for that but prefer the concentrate. This antibody is supplied by Sigma-Aldrich and is backordered for a few weeks, and we have a deadline to meet, so I need it asap. Thanks for any help or suggestions! Happy Friday, Becky. Becky Orr CLA,HT(ASCP)QIHC Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 From MDiCarlo <@t> KaleidaHealth.Org Fri Aug 1 07:23:50 2008 From: MDiCarlo <@t> KaleidaHealth.Org (DiCarlo, Margaret) Date: Fri Aug 1 07:24:02 2008 Subject: [Histonet] knife sharpener lapping plate Message-ID: <1B73766A27A1554CB2729B6432E81301B2F9CA@KALEXMB04.KaleidaHealth.org> Histonetters, For those of you who still use the Shandon Autosharp 5 for knife sharpening like me, how do you know when you need to replace the lapping plates? Thanks. Peggy DiCarlo (HT) Ortho Bone Lab Buffalo General Hospital 716-859-1293 2007 Best Places to Work Finalist Visit our careers page at www.kaleidahealth.org/careers CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From akbitting <@t> geisinger.edu Fri Aug 1 07:34:06 2008 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Aug 1 07:34:20 2008 Subject: [Histonet] Skin biopsies and other small biopsy specimens are placed in a tissue processor tray at the time of gross dictation in the surgical pathology laboratory. Message-ID: <4892CA7D.2B7F.00C9.0@geisinger.edu> Skin biopsies and other small biopsy specimens are placed in a tissue processor tray at the time of gross dictation in the surgical pathology laboratory. The metal fenestrated tissue processor tray is sitting in a Tupperware container that contains formalin. If sufficient formalin is added to the Tupperware container to completely immerse the accumulating tissue cassettes, the cassettes float out of the rack. Because it is inconvenient to repeatly remove a metal lid, the level of formalin in the container is kept low enough so that the weight of the cassette keeps it from floating. The problem is that the cassettes are then not immersed in formalin and the level of formalin bathing them is sometimes very low. How do others rack cassettes at the grossing bench. Is there a tray that will hold the cassettes in place and allow them to be submerged in the formalin without the use of a removable lid? These trays would be for the V.I.P. processors. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD From rjbuesa <@t> yahoo.com Fri Aug 1 07:47:36 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 1 07:47:39 2008 Subject: [Histonet] Number of blocks per case In-Reply-To: <397958B23C3E4D43AB50099AE6313C382FEF0D@slhmailsvr.slhn.org> Message-ID: <659550.68639.qm@web65712.mail.ac4.yahoo.com> The number of blocks/case depend more on who is grossing than in the general setting. Residents use to put through more blocks, as well as in teaching institutions. Large reference labs, trying to expedite things and reduce the TAT, have less blocks/case. The number vary from 1.3 to 5.0, with an average of 2.8 blocks/case Ren? J. --- On Thu, 7/31/08, Artim, Kimberly wrote: From: Artim, Kimberly Subject: [Histonet] Number of blocks per case To: histonet@lists.utsouthwestern.edu Date: Thursday, July 31, 2008, 3:05 PM Could anyone share with me the average # of blocks per case at their institution. I would like figures from hospitals comparable to ours. We are a city hospital with approximately 25,000 routine surgical cases per year. We receive a wide range of specimens. 25% are derm, 25% are GI biopsies and the rest are complex specimens. Thank you in advance for your help. Kim Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Fri Aug 1 09:11:07 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Aug 1 09:11:16 2008 Subject: [Histonet] Skin biopsies and other small biopsy specimens areplaced in atissue processor tray at the time of gross dictation in thesurgical pathology laboratory. In-Reply-To: <4892CA7D.2B7F.00C9.0@geisinger.edu> References: <4892CA7D.2B7F.00C9.0@geisinger.edu> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82D65@EMAIL.archildrens.org> The trays for the ASP 300 tissue processor by Leica hold the cassette in place when the separator 'spring' is in place. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, August 01, 2008 7:34 AM To: histonet; Histonet-requests@lists.utsouthwestern.edu Subject: [Histonet] Skin biopsies and other small biopsy specimens areplaced in atissue processor tray at the time of gross dictation in thesurgical pathology laboratory. Skin biopsies and other small biopsy specimens are placed in a tissue processor tray at the time of gross dictation in the surgical pathology laboratory. The metal fenestrated tissue processor tray is sitting in a Tupperware container that contains formalin. If sufficient formalin is added to the Tupperware container to completely immerse the accumulating tissue cassettes, the cassettes float out of the rack. Because it is inconvenient to repeatly remove a metal lid, the level of formalin in the container is kept low enough so that the weight of the cassette keeps it from floating. The problem is that the cassettes are then not immersed in formalin and the level of formalin bathing them is sometimes very low. How do others rack cassettes at the grossing bench. Is there a tray that will hold the cassettes in place and allow them to be submerged in the formalin without the use of a removable lid? These trays would be for the V.I.P. processors. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From rfields <@t> gidocs.net Fri Aug 1 09:22:25 2008 From: rfields <@t> gidocs.net (Rosa Fields) Date: Fri Aug 1 09:22:30 2008 Subject: [Histonet] Skin biopsies and other small biopsy specimensareplaced in atissue processor tray at the time of grossdictation in thesurgical pathology laboratory. In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82D65@EMAIL.archildrens.org> References: <4892CA7D.2B7F.00C9.0@geisinger.edu> <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82D65@EMAIL.archildrens.org> Message-ID: <2F2611250DCD6549AA3D96CE8AF1F018012205BB@giexchange.gidocs.net> We use the McCormick Scientific biopsy cassettes (microflow), they are great, the design keeps them from floating.. Would be a simple fix! We buy them from statlab and VWR.. Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Friday, August 01, 2008 9:11 AM To: Angela Bitting; histonet; Histonet-requests@lists.utsouthwestern.edu Subject: RE: [Histonet] Skin biopsies and other small biopsy specimensareplaced in atissue processor tray at the time of grossdictation in thesurgical pathology laboratory. The trays for the ASP 300 tissue processor by Leica hold the cassette in place when the separator 'spring' is in place. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, August 01, 2008 7:34 AM To: histonet; Histonet-requests@lists.utsouthwestern.edu Subject: [Histonet] Skin biopsies and other small biopsy specimens areplaced in atissue processor tray at the time of gross dictation in thesurgical pathology laboratory. Skin biopsies and other small biopsy specimens are placed in a tissue processor tray at the time of gross dictation in the surgical pathology laboratory. The metal fenestrated tissue processor tray is sitting in a Tupperware container that contains formalin. If sufficient formalin is added to the Tupperware container to completely immerse the accumulating tissue cassettes, the cassettes float out of the rack. Because it is inconvenient to repeatly remove a metal lid, the level of formalin in the container is kept low enough so that the weight of the cassette keeps it from floating. The problem is that the cassettes are then not immersed in formalin and the level of formalin bathing them is sometimes very low. How do others rack cassettes at the grossing bench. Is there a tray that will hold the cassettes in place and allow them to be submerged in the formalin without the use of a removable lid? These trays would be for the V.I.P. processors. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bill501 <@t> mindspring.com Fri Aug 1 09:43:54 2008 From: bill501 <@t> mindspring.com (Bill) Date: Fri Aug 1 09:44:07 2008 Subject: [Histonet] Number of blocks per case In-Reply-To: <397958B23C3E4D43AB50099AE6313C382FEF0D@slhmailsvr.slhn.org> References: <397958B23C3E4D43AB50099AE6313C382FEF0D@slhmailsvr.slhn.org> Message-ID: At 3:05 PM -0400 7/31/08, Artim, Kimberly wrote: >Could anyone share with me the average # of blocks per case at their institution. I would like figures from hospitals comparable to ours. We are a city hospital with approximately 25,000 routine surgical cases per year. We receive a wide range of specimens. 25% are derm, 25% are GI biopsies and the rest are complex specimens. Thank you in advance for your help. Just curious... Of what possible use is such a number? BB From kalschev <@t> svm.vetmed.wisc.edu Fri Aug 1 09:56:38 2008 From: kalschev <@t> svm.vetmed.wisc.edu (Vicki Kalscheur) Date: Fri Aug 1 09:56:57 2008 Subject: [Histonet] polarization - Liz Message-ID: <002f01c8f3e6$cd0fede0$c5d76880@vetmed.wisc.edu> To my knowledge, there is one type of polarization. There is an attachment and analyzer that fits onto the transmitted light microscope. It should have a first order red plate that works well for connective tissue. Vicki From NMargaryan <@t> childrensmemorial.org Fri Aug 1 12:03:09 2008 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Fri Aug 1 12:03:47 2008 Subject: [Histonet] Rabbit IgG control Message-ID: Hi Dears, I have to order a Rabbit IgG control. What company is making best Rabbit IgG control, not universal, please. Thanks in advance, Naira Naira V. Margaryan, D.V.M., Ph.D. Research Scientist Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3363 Tel: 773-755-6340 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 From Keith.Danielson <@t> uphs.upenn.edu Fri Aug 1 12:07:21 2008 From: Keith.Danielson <@t> uphs.upenn.edu (Danielson, Keith) Date: Fri Aug 1 12:07:48 2008 Subject: [Histonet] Alginate beads containing cells Message-ID: <84D11048BB18234D8A56474645E8EC020829EF6F@uphsmbx5.UPHS.PENNHEALTH.PRV> Hello everyone, I am requesting information for a graduate student on how to fix and process alginate beads with embedded tissue culture cells for paraffin histology. The beads are fixed in 4% paraformaldehyde but seem to turn white and disintegrate during routine processing. Thanks very much, Keith Danielson, PhD Supervisor, IHC Lab Pennsylvania Hospital Philadelphia, PA 19107 Phone: 215 829-7726 or 829-3696 E-mail: keith.danielson@uphs.upenn.edu The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From amber.mckenzie <@t> gastrodocs.net Fri Aug 1 12:23:42 2008 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Fri Aug 1 12:23:46 2008 Subject: [Histonet] HT schools Message-ID: <03C921A1EAF7F541B16543F6EC6A4B3701D3AEBF@giamail2.Gia.com> Where are all the HT accredited schools and why aren't there more out there? I've seen the online classes' people can take, but that requires them to be trained in a lab, as well, for the "hands on" part. So, actually the supervisor still has to train potential HT's "on the job" before they can sit for the board exam. Right? From christopher.hayden <@t> novartis.com Fri Aug 1 12:41:00 2008 From: christopher.hayden <@t> novartis.com (christopher.hayden@novartis.com) Date: Fri Aug 1 12:41:09 2008 Subject: [Histonet] Christopher Hayden is nowhere near the lab. Message-ID: I will be out of the office starting 08/01/2008 and will not return until 08/11/2008. Good Day Everyone! I will be out of the lab from 2 August until 10 August. I'll be back at my desk on the morning of the 11th. I will have no access to corporate email during this time. If you have an EM-related matter, please contact the Lab Manager, Gregory Argentieri, at x2-8617 Otherwise, I'll get back to you on the 11th or shortly afterwards. Thanks! -CH From bakevictoria <@t> gmail.com Fri Aug 1 13:13:13 2008 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Fri Aug 1 13:13:19 2008 Subject: [Histonet] A question for BondMax users Message-ID: <4f016b690808011113k4bceed66w3a76c8bb38fa8cb@mail.gmail.com> Happy Friday everyone! In the BondMax detection system there is a "post primary" step that contains 10% BSA in a Tris buffer. I've tested the protocol excluding this step and did not have any staining. I can't seem to get the answer I'm looking for from the company, so if anyone can help me I'd really appreciate it. Thanks Vikki From bakevictoria <@t> gmail.com Fri Aug 1 13:18:43 2008 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Fri Aug 1 13:18:49 2008 Subject: [Histonet] HT schools In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B3701D3AEBF@giamail2.Gia.com> References: <03C921A1EAF7F541B16543F6EC6A4B3701D3AEBF@giamail2.Gia.com> Message-ID: <4f016b690808011118k34ecf6c5r20c4960d00b679c6@mail.gmail.com> Hi Amber, If you go to the NSH website there are schools listed there. I'm an alumni of the SUNY school at Cobleskill - and I loved it - but it was also thirty years ago. I would check that list and see if you can't make some calls or e-mails to their co-ordinators. Regards, Vikki Baker On 8/1/08, Amber McKenzie wrote: > Where are all the HT accredited schools and why aren't there more out > there? I've seen the online classes' people can take, but that requires > them to be trained in a lab, as well, for the "hands on" part. So, > actually the supervisor still has to train potential HT's "on the job" > before they can sit for the board exam. Right? > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From SLB <@t> Stowers-Institute.org Fri Aug 1 13:27:36 2008 From: SLB <@t> Stowers-Institute.org (Beckham, Sharon) Date: Fri Aug 1 13:27:48 2008 Subject: [Histonet] A question for BondMax users In-Reply-To: <4f016b690808011113k4bceed66w3a76c8bb38fa8cb@mail.gmail.com> Message-ID: Hi Vikki, I don't know if I'm much help on this because I just started testing the BondMax this week so I'm just in the learning process too. As far as I can tell the post primary is for blocking, but also works in conjunction with the polymer which is the following step. It supposedly also enhances penetration of the polymer, so they both have to be used together. Hope this helps. Sharon -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Friday, August 01, 2008 1:13 PM To: Histo Net list server Subject: [Histonet] A question for BondMax users Happy Friday everyone! In the BondMax detection system there is a "post primary" step that contains 10% BSA in a Tris buffer. I've tested the protocol excluding this step and did not have any staining. I can't seem to get the answer I'm looking for from the company, so if anyone can help me I'd really appreciate it. Thanks Vikki _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brewerd <@t> nacmem.org Fri Aug 1 13:27:28 2008 From: brewerd <@t> nacmem.org (Dana Brewer) Date: Fri Aug 1 14:29:29 2008 Subject: [Histonet] Bone Marrow Biopsy Needles Message-ID: <000601c8f404$619b66c0$0b0a10ac@work.nmh.org> Does anyone have a replacement source for J Style Needle, -Marrow Loc- Bone Marrow Biopsy Needles -- no longer manufactured by US BIOPSY -- the replacement we got is not the same "marrow loc" system our oncologist prefers. Thanks for your help From rjbuesa <@t> yahoo.com Fri Aug 1 14:42:14 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 1 14:42:19 2008 Subject: [Histonet] HT schools In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B3701D3AEBF@giamail2.Gia.com> Message-ID: <78031.12752.qm@web65716.mail.ac4.yahoo.com> Wrong! The advantage of the "on line" or "distance learning" courses is that they provide the theory on line while you are working at a given laboratory doing your training (or even as part of your daily work) so there is no "actual training" to be done by the supervisor. At this moment NAACLS has 30 HT and 3 HTL accredited programs only, with an overall capacity of about 300-325 students, and this will not be enough to take care of? all the retiring histotechs. Costs is one of the reasons why the number of HTs schools is dwindling. Ren? J. ? --- On Fri, 8/1/08, Amber McKenzie wrote: From: Amber McKenzie Subject: [Histonet] HT schools To: histonet@lists.utsouthwestern.edu Date: Friday, August 1, 2008, 1:23 PM Where are all the HT accredited schools and why aren't there more out there? I've seen the online classes' people can take, but that requires them to be trained in a lab, as well, for the "hands on" part. So, actually the supervisor still has to train potential HT's "on the job" before they can sit for the board exam. Right? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Fri Aug 1 16:23:31 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Fri Aug 1 16:23:41 2008 Subject: [Histonet] processing PFA fixed alginate beads Message-ID: <000801c8f41c$d9196180$6401a8c0@Sunney> I Googled this subject and brought up several publications using alginate beads embedded in paraffin. HOwever, the following publication, using cryotomy, had better results than using paraffin sections. Also, Yang C-C, et al. Adapted cyrosectioning method for hydrogels used in regenerative medicine. J Histotechnology 30(3):185-191, 2007. Linda Jenkins, on of the co-authors, was a huge part of this, has much experience with hydrogels. In this publication, they fabricated their own alginate beads. The reference list is extensive and informative. Good Luck Gayle M. Callis HTL/HT/MT(ASCP) -------------------------------------------------------------------------------- From gayle.callis <@t> bresnan.net Fri Aug 1 16:28:53 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Fri Aug 1 16:29:00 2008 Subject: [Histonet] Methodology for extracting nucleic acids from FFPE Tissue Message-ID: <000f01c8f41d$990a30f0$6401a8c0@Sunney> For the person needing a protocol for extracting DNA and/or RNA from breast (?) tissue, check out QuickExtract FFPE DNA and RNA Extraction Kits from Epicenter Biotechnologies, www. EpiBio.com Phone # is 800 284-8474. This information was found in the latest issue of Science ( Life ScienCe Technologies section) Gayle M. Callis HTL/HT/MT(ASCP) From mike <@t> pathview.com Fri Aug 1 17:14:56 2008 From: mike <@t> pathview.com (Michael Mihalik) Date: Fri Aug 1 17:15:43 2008 Subject: [Histonet] Dictation systems In-Reply-To: <366247.75524.qm@web59902.mail.ac4.yahoo.com> References: <366247.75524.qm@web59902.mail.ac4.yahoo.com> Message-ID: <001501c8f424$204a2830$60de7890$@com> Jon, did you ever get an answer to this question? I am not as familiar with all the various dictation systems that I would like to be, but I have used something that may help. You can buy a foot pedal that can be 'programmed' to send a hotkey combination to an external device, typically a PC. In my case, I was considering using this setup to enable and disable Dragon Naturally Speaking at the gross workstation. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jon Google Sent: Tuesday, July 29, 2008 3:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dictation systems We are in the process of upgrading our LIS. We are looking at moving our dictation system from our current Dictaphone analog system. We would like to use a digital system that can support VOIP.? The problem we are running into is trying to find one that supports a hands free control that our grossing pathologists need. ? Does anyone have recommendations on digital systems that support foot controls? ? Thanks Jon _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Sat Aug 2 07:19:36 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sat Aug 2 07:19:00 2008 Subject: [Histonet] HT schools References: <78031.12752.qm@web65716.mail.ac4.yahoo.com> Message-ID: <002c01c8f49a$07995790$e797b348@yourxhtr8hvc4p> our one community college program in San Antonio is in jeopardy of closing because it can't get the required 10 students to make a class. If this program does close, I will be willing to work with a college or university with long- distance learning. I have 5 openings in my lab. JTT ----- Original Message ----- From: "Rene J Buesa" To: ; "Amber McKenzie" Sent: Friday, August 01, 2008 2:42 PM Subject: Re: [Histonet] HT schools Wrong! The advantage of the "on line" or "distance learning" courses is that they provide the theory on line while you are working at a given laboratory doing your training (or even as part of your daily work) so there is no "actual training" to be done by the supervisor. At this moment NAACLS has 30 HT and 3 HTL accredited programs only, with an overall capacity of about 300-325 students, and this will not be enough to take care of all the retiring histotechs. Costs is one of the reasons why the number of HTs schools is dwindling. Ren? J. --- On Fri, 8/1/08, Amber McKenzie wrote: From: Amber McKenzie Subject: [Histonet] HT schools To: histonet@lists.utsouthwestern.edu Date: Friday, August 1, 2008, 1:23 PM Where are all the HT accredited schools and why aren't there more out there? I've seen the online classes' people can take, but that requires them to be trained in a lab, as well, for the "hands on" part. So, actually the supervisor still has to train potential HT's "on the job" before they can sit for the board exam. Right? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Sun Aug 3 07:42:30 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Sun Aug 3 07:42:41 2008 Subject: [Histonet] Dictation systems In-Reply-To: <001501c8f424$204a2830$60de7890$@com> References: <366247.75524.qm@web59902.mail.ac4.yahoo.com> <001501c8f424$204a2830$60de7890$@com> Message-ID: <982A0A9461F9BF438C7B19A6E425A383475B42@ITSSSXM01V6.one.ads.che.org> We are in the process of purchasing Dragon as we speak. The headsets are voice activated so I am counting on that working in the gross room, although I have included a foot pedal in the purchase request which will activate the head set. I will know more next week when it is installed. Let me know if you would like follow up and I'll keep you posted. I can't imagine yet how they will gross and edit at the same time... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Friday, August 01, 2008 6:15 PM To: ht.ascp@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dictation systems Jon, did you ever get an answer to this question? I am not as familiar with all the various dictation systems that I would like to be, but I have used something that may help. You can buy a foot pedal that can be 'programmed' to send a hotkey combination to an external device, typically a PC. In my case, I was considering using this setup to enable and disable Dragon Naturally Speaking at the gross workstation. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jon Google Sent: Tuesday, July 29, 2008 3:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dictation systems We are in the process of upgrading our LIS. We are looking at moving our dictation system from our current Dictaphone analog system. We would like to use a digital system that can support VOIP.? The problem we are running into is trying to find one that supports a hands free control that our grossing pathologists need. ? Does anyone have recommendations on digital systems that support foot controls? ? Thanks Jon _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From marktarango <@t> gmail.com Sun Aug 3 07:47:30 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Sun Aug 3 07:47:36 2008 Subject: [Histonet] Dictation systems In-Reply-To: <982A0A9461F9BF438C7B19A6E425A383475B42@ITSSSXM01V6.one.ads.che.org> References: <366247.75524.qm@web59902.mail.ac4.yahoo.com> <001501c8f424$204a2830$60de7890$@com> <982A0A9461F9BF438C7B19A6E425A383475B42@ITSSSXM01V6.one.ads.che.org> Message-ID: <5b6eb13e0808030547s4d641f05y66627e7f6e1c10ad@mail.gmail.com> You just tell it backspace... it's pretty good. You might want to spend the time training it to your voice and to recognize all the pathology words though. On 8/3/08, Weems, Joyce wrote: > > We are in the process of purchasing Dragon as we speak. The headsets are > voice activated so I am counting on that working in the gross room, although > I have included a foot pedal in the purchase request which will activate the > head set. I will know more next week when it is installed. Let me know if > you would like follow up and I'll keep you posted. > > I can't imagine yet how they will gross and edit at the same time... > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital > 5665 Peachtree Dunwoody Rd NE > Atlanta, GA 30342 > 404-851-7376 - Phone > 404-851-7831 - Fax > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik > Sent: Friday, August 01, 2008 6:15 PM > To: ht.ascp@yahoo.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Dictation systems > > Jon, did you ever get an answer to this question? > > I am not as familiar with all the various dictation systems that I would > like to be, but I have used something that may help. > > You can buy a foot pedal that can be 'programmed' to send a hotkey > combination to an external device, typically a PC. > > In my case, I was considering using this setup to enable and disable > Dragon Naturally Speaking at the gross workstation. > > Michael Mihalik > PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jon Google > Sent: Tuesday, July 29, 2008 3:29 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Dictation systems > > We are in the process of upgrading our LIS. We are looking at moving our > dictation system from our current Dictaphone analog system. We would like to > use a digital system that can support VOIP. The problem we are running into > is trying to find one that supports a hands free control that our grossing > pathologists need. > > Does anyone have recommendations on digital systems that support foot > controls? > > Thanks > Jon > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This email, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please reply to the > sender that you have received the message in > error, then delete this message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mike <@t> pathview.com Sun Aug 3 07:53:31 2008 From: mike <@t> pathview.com (Michael Mihalik) Date: Sun Aug 3 07:53:42 2008 Subject: [Histonet] Dictation systems In-Reply-To: <982A0A9461F9BF438C7B19A6E425A383475B42@ITSSSXM01V6.one.ads.che.org> References: <366247.75524.qm@web59902.mail.ac4.yahoo.com> <001501c8f424$204a2830$60de7890$@com> <982A0A9461F9BF438C7B19A6E425A383475B42@ITSSSXM01V6.one.ads.che.org> Message-ID: <015901c8f567$ef964b20$cec2e160$@com> Can you explain what you mean when you say the 'headsets are voice activated'? Also, have you ever used Dragon before? There's a difference between 'go to sleep' and 'microphone off'. Where did you get the foot pedal from and how does it work, if you don't mind my asking? I'd be curious what other people's experience with Dragon is, if you can spare the time. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: Weems, Joyce [mailto:JWeems@sjha.org] Sent: Sunday, August 03, 2008 8:42 AM To: Michael Mihalik; ht.ascp@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dictation systems We are in the process of purchasing Dragon as we speak. The headsets are voice activated so I am counting on that working in the gross room, although I have included a foot pedal in the purchase request which will activate the head set. I will know more next week when it is installed. Let me know if you would like follow up and I'll keep you posted. I can't imagine yet how they will gross and edit at the same time... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Friday, August 01, 2008 6:15 PM To: ht.ascp@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dictation systems Jon, did you ever get an answer to this question? I am not as familiar with all the various dictation systems that I would like to be, but I have used something that may help. You can buy a foot pedal that can be 'programmed' to send a hotkey combination to an external device, typically a PC. In my case, I was considering using this setup to enable and disable Dragon Naturally Speaking at the gross workstation. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jon Google Sent: Tuesday, July 29, 2008 3:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dictation systems We are in the process of upgrading our LIS. We are looking at moving our dictation system from our current Dictaphone analog system. We would like to use a digital system that can support VOIP.? The problem we are running into is trying to find one that supports a hands free control that our grossing pathologists need. ? Does anyone have recommendations on digital systems that support foot controls? ? Thanks Jon _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From JWeems <@t> sjha.org Sun Aug 3 12:22:48 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Sun Aug 3 12:22:55 2008 Subject: [Histonet] Dictation systems In-Reply-To: <5b6eb13e0808030547s4d641f05y66627e7f6e1c10ad@mail.gmail.com> References: <366247.75524.qm@web59902.mail.ac4.yahoo.com> <001501c8f424$204a2830$60de7890$@com> <982A0A9461F9BF438C7B19A6E425A383475B42@ITSSSXM01V6.one.ads.che.org> <5b6eb13e0808030547s4d641f05y66627e7f6e1c10ad@mail.gmail.com> Message-ID: <982A0A9461F9BF438C7B19A6E425A383475B56@ITSSSXM01V6.one.ads.che.org> We have training set up. Hopefully it won't take too long! ________________________________ From: Mark Tarango [mailto:marktarango@gmail.com] Sent: Sunday, August 03, 2008 8:48 AM To: Weems, Joyce Cc: Michael Mihalik; ht.ascp@yahoo.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Dictation systems You just tell it backspace... it's pretty good. You might want to spend the time training it to your voice and to recognize all the pathology words though. On 8/3/08, Weems, Joyce wrote: We are in the process of purchasing Dragon as we speak. The headsets are voice activated so I am counting on that working in the gross room, although I have included a foot pedal in the purchase request which will activate the head set. I will know more next week when it is installed. Let me know if you would like follow up and I'll keep you posted. I can't imagine yet how they will gross and edit at the same time... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Friday, August 01, 2008 6:15 PM To: ht.ascp@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dictation systems Jon, did you ever get an answer to this question? I am not as familiar with all the various dictation systems that I would like to be, but I have used something that may help. You can buy a foot pedal that can be 'programmed' to send a hotkey combination to an external device, typically a PC. In my case, I was considering using this setup to enable and disable Dragon Naturally Speaking at the gross workstation. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jon Google Sent: Tuesday, July 29, 2008 3:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dictation systems We are in the process of upgrading our LIS. We are looking at moving our dictation system from our current Dictaphone analog system. We would like to use a digital system that can support VOIP. The problem we are running into is trying to find one that supports a hands free control that our grossing pathologists need. Does anyone have recommendations on digital systems that support foot controls? Thanks Jon _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From kappeler <@t> patho.unibe.ch Mon Aug 4 01:17:18 2008 From: kappeler <@t> patho.unibe.ch (Andi Kappeler) Date: Mon Aug 4 01:17:30 2008 Subject: AW: [Histonet] A question for BondMax users In-Reply-To: <4f016b690808011113k4bceed66w3a76c8bb38fa8cb@mail.gmail.com> References: <4f016b690808011113k4bceed66w3a76c8bb38fa8cb@mail.gmail.com> Message-ID: <005d01c8f5f9$bf7002f0$17955c82@pi23> Hi Vikki about a year ago we were testing a visualization system that probably came from the 'same kitchen' as does your stuff. It also contained a 'post primary block'. When you omitted the post primary while using rabbit primaries, you had good staining, however, when you did the same while using mouse primaries you've got nothing. In the old days, we used to call this 'post primary block' a secondary antibody: the polymer in the visualization system tested was anti-rabbit only, so you had to 'turn' all your mouse primaries into 'rabbits' by adding a rabbit-a-mouse secondary antibody. But, of course, a visualization system that contains an extra-enhancing intergalactic superduperwhatsoever post primary block sounds a lot better than something that contains such a simple thing as a secondary antibody ... If you repeat our experiment and may be run a few slides with mouse primaries with and without 'post primary block' as well as with a rabbit-a-mouse secondary of your choice (it may even be biotinylated, should still work) you may be able to tell, whether your 'post primary block' has the same function as did ours. Good luck! Best regards Andi Kappeler Institute of Pathology, University of Bern, Switzerland -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Victoria Baker Gesendet: Freitag, 1. August 2008 20:13 An: Histo Net list server Betreff: [Histonet] A question for BondMax users Happy Friday everyone! In the BondMax detection system there is a "post primary" step that contains 10% BSA in a Tris buffer. I've tested the protocol excluding this step and did not have any staining. I can't seem to get the answer I'm looking for from the company, so if anyone can help me I'd really appreciate it. Thanks Vikki _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Pathrm35 <@t> comcast.net Mon Aug 4 05:21:10 2008 From: Pathrm35 <@t> comcast.net (Pathrm35@comcast.net) Date: Mon Aug 4 05:21:16 2008 Subject: [Histonet] surveillance cameras in the lab Message-ID: <080420081021.7972.4896D816000E927500001F242212020784CACC039D089B0EAF@comcast.net> I was wondering how many techs out there have cameras in their labs, either for security or to monitor employees. I went to work Sunday night and noticed that 4 cameras were installed in the lab over the weekend, with more to come. From jnocito <@t> satx.rr.com Mon Aug 4 05:46:43 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Aug 4 05:46:48 2008 Subject: [Histonet] surveillance cameras in the lab References: <080420081021.7972.4896D816000E927500001F242212020784CACC039D089B0EAF@comcast.net> Message-ID: <001a01c8f61f$631e2c40$e797b348@yourxhtr8hvc4p> has theft been a problem? If not, this is the first case of big brother in the lab that I've heard of. Pretty sad if you ask me. Oh yeah, you did ask. Stuff like this just curls my toenails. JTT ----- Original Message ----- From: To: Sent: Monday, August 04, 2008 5:21 AM Subject: [Histonet] surveillance cameras in the lab >I was wondering how many techs out there have cameras in their labs, either >for security or to monitor employees. I went to work Sunday night and >noticed that 4 cameras were installed in the lab over the weekend, with >more to come. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Pathrm35 <@t> comcast.net Mon Aug 4 05:58:20 2008 From: Pathrm35 <@t> comcast.net (Pathrm35@comcast.net) Date: Mon Aug 4 05:58:23 2008 Subject: [Histonet] surveillance cameras in the lab Message-ID: <080420081058.8160.4896E0CB000EB92800001FE02212020784CACC039D089B0EAF@comcast.net> No problems with theft or problems with employees. Maybe to monitor the workload? Can't seem to get an answer. -------------- Original message -------------- From: "Joe Nocito" > has theft been a problem? If not, this is the first case of big brother in > the lab that I've heard of. Pretty sad if you ask me. Oh yeah, you did ask. > Stuff like this just curls my toenails. > > JTT > ----- Original Message ----- > From: > To: > Sent: Monday, August 04, 2008 5:21 AM > Subject: [Histonet] surveillance cameras in the lab > > > >I was wondering how many techs out there have cameras in their labs, either > >for security or to monitor employees. I went to work Sunday night and > >noticed that 4 cameras were installed in the lab over the weekend, with > >more to come. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barry.R.Rittman <@t> uth.tmc.edu Mon Aug 4 06:05:20 2008 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Mon Aug 4 06:05:24 2008 Subject: [Histonet] surveillance cameras in the lab References: <080420081021.7972.4896D816000E927500001F242212020784CACC039D089B0EAF@comcast.net> Message-ID: In my experience this type of situation occurs when a situation has arisen and the administration does not know how to take care of it directly. If there is such a problem then the administration needs to discuss this at least with the supervisors and preferably with all the employees. In a lab, there must be two way trust for a team to work. Seems to me that this trust has just been lost. The question is also of what happens to any recordings. I can see many abuses of such a system. Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Pathrm35@comcast.net Sent: Mon 8/4/2008 5:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] surveillance cameras in the lab I was wondering how many techs out there have cameras in their labs, either for security or to monitor employees. I went to work Sunday night and noticed that 4 cameras were installed in the lab over the weekend, with more to come. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Mon Aug 4 06:08:44 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Mon Aug 4 06:09:01 2008 Subject: [Histonet] surveillance cameras in the lab In-Reply-To: <080420081058.8160.4896E0CB000EB92800001FE02212020784CACC039D089B0EAF@comcast.net> References: <080420081058.8160.4896E0CB000EB92800001FE02212020784CACC039D089B0EAF@comcast.net> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A7F23E1C@LTA3VS011.ees.hhs.gov> Many years ago I worked in a small hospital in a high-crime area. There were plans to install cameras for security reasons but I left before it was implemented. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathrm35@comcast.net Sent: Monday, August 04, 2008 6:58 AM To: Joe Nocito; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] surveillance cameras in the lab No problems with theft or problems with employees. Maybe to monitor the workload? Can't seem to get an answer. -------------- Original message -------------- From: "Joe Nocito" > has theft been a problem? If not, this is the first case of big > brother in the lab that I've heard of. Pretty sad if you ask me. Oh yeah, you did ask. > Stuff like this just curls my toenails. > > JTT > ----- Original Message ----- > From: > To: > Sent: Monday, August 04, 2008 5:21 AM > Subject: [Histonet] surveillance cameras in the lab > > > >I was wondering how many techs out there have cameras in their labs, > >either for security or to monitor employees. I went to work Sunday > >night and noticed that 4 cameras were installed in the lab over the > >weekend, with more to come. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Mon Aug 4 07:02:18 2008 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Mon Aug 4 07:02:23 2008 Subject: [Histonet] surveillance cameras in the lab Message-ID: <708584.1865.qm@web50109.mail.re2.yahoo.com> If no one will explain, I'd find a new job. Today! Histotechs are in such short supply, you can go anywhere you want. ?Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 631 N. Broadway Ave. Moore, OK 73160 405-570-6679 cell 405-759-3953 lab contact@excaliburpathology.com www.excaliburpathology.com From DDittus787 <@t> aol.com Mon Aug 4 07:16:19 2008 From: DDittus787 <@t> aol.com (DDittus787@aol.com) Date: Mon Aug 4 07:16:31 2008 Subject: [Histonet] surveillance cameras in the lab Message-ID: I work in a lab that has an irradiator for blood products, it is under camera eye and we who use it have been fingerprinted and credit checked-i was told this was an anti-terrorism move so that those of us with good credit had no reason or motive to help make the b word! Dana Dittus In a message dated 8/4/2008 6:58:51 A.M. Eastern Daylight Time, Pathrm35@comcast.net writes: No problems with theft or problems with employees. Maybe to monitor the workload? Can't seem to get an answer. -------------- Original message -------------- From: "Joe Nocito" > has theft been a problem? If not, this is the first case of big brother in > the lab that I've heard of. Pretty sad if you ask me. Oh yeah, you did ask. > Stuff like this just curls my toenails. > > JTT > ----- Original Message ----- > From: > To: > Sent: Monday, August 04, 2008 5:21 AM > Subject: [Histonet] surveillance cameras in the lab > > > >I was wondering how many techs out there have cameras in their labs, either > >for security or to monitor employees. I went to work Sunday night and > >noticed that 4 cameras were installed in the lab over the weekend, with > >more to come. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************Looking for a car that's sporty, fun and fits in your budget? Read reviews on AOL Autos. (http://autos.aol.com/cars-BMW-128-2008/expert-review?ncid=aolaut00050000000017 ) From GDawson <@t> dynacaremilwaukee.com Mon Aug 4 07:51:04 2008 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Mon Aug 4 07:51:12 2008 Subject: [Histonet] surveillance cameras in the lab In-Reply-To: <080420081058.8160.4896E0CB000EB92800001FE02212020784CACC039D089B0EAF@comcast.net> Message-ID: I would suggest that your institution take the $$ that they will flush on the surveillance equipment and on the knuckle dragger that sits in front of the screens to spy on the techs & give it to the histo-staff as a bonus. This would have a much more positive effect on workflow than the "1984" approach. Another lab should be considered as I doubt that it will stop there. Best of Luck, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Pathrm35@comcast.net Sent: Monday, August 04, 2008 5:58 AM To: Joe Nocito; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] surveillance cameras in the lab No problems with theft or problems with employees. Maybe to monitor the workload? Can't seem to get an answer. -------------- Original message -------------- From: "Joe Nocito" > has theft been a problem? If not, this is the first case of big brother in > the lab that I've heard of. Pretty sad if you ask me. Oh yeah, you did ask. > Stuff like this just curls my toenails. > > JTT > ----- Original Message ----- > From: > To: > Sent: Monday, August 04, 2008 5:21 AM > Subject: [Histonet] surveillance cameras in the lab > > > >I was wondering how many techs out there have cameras in their labs, either > >for security or to monitor employees. I went to work Sunday night and > >noticed that 4 cameras were installed in the lab over the weekend, with > >more to come. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From S.MacPherson <@t> hrsu.mrc.ac.uk Mon Aug 4 07:58:40 2008 From: S.MacPherson <@t> hrsu.mrc.ac.uk (Sheila MacPherson) Date: Mon Aug 4 07:59:16 2008 Subject: [Histonet] RE: A question for BondMax users [Scanned] In-Reply-To: References: Message-ID: <86F334797DC6524A99AD9DD8F23A8B50111A61@mailserv.hrsu.mrc.ac.uk> We have been using a Bond immunostaining system for several years now. The pre polymer is a rabbit anti mouse linker, to allow the detection of either rabbit or mouse antibodies. The polymer is anti rabbit so if you are using a mouse primary you will need the pre polymer and for a rabbit primary the pre polymer can be edited out of the protocol (can reduce background if it is a problem) For goat primaries we substitute a rabbit anti goat secondary link instead of the pre polymer. Sheila -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: 02 August 2008 18:06 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 57, Issue 2 [Scanned] Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Rabbit IgG control (Margaryan, Naira) 2. Alginate beads containing cells (Danielson, Keith) 3. HT schools (Amber McKenzie) 4. Christopher Hayden is nowhere near the lab. (christopher.hayden@novartis.com) 5. A question for BondMax users (Victoria Baker) 6. Re: HT schools (Victoria Baker) 7. RE: A question for BondMax users (Beckham, Sharon) 8. Bone Marrow Biopsy Needles (Dana Brewer) 9. Re: HT schools (Rene J Buesa) 10. processing PFA fixed alginate beads (Gayle Callis) 11. Methodology for extracting nucleic acids from FFPE Tissue (Gayle Callis) 12. RE: Dictation systems (Michael Mihalik) 13. Re: HT schools (Joe Nocito) ---------------------------------------------------------------------- Message: 1 Date: Fri, 1 Aug 2008 12:03:09 -0500 From: "Margaryan, Naira" Subject: [Histonet] Rabbit IgG control To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Dears, I have to order a Rabbit IgG control. What company is making best Rabbit IgG control, not universal, please. Thanks in advance, Naira Naira V. Margaryan, D.V.M., Ph.D. Research Scientist Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3363 Tel: 773-755-6340 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 ------------------------------ Message: 2 Date: Fri, 1 Aug 2008 13:07:21 -0400 From: "Danielson, Keith" Subject: [Histonet] Alginate beads containing cells To: Message-ID: <84D11048BB18234D8A56474645E8EC020829EF6F@uphsmbx5.UPHS.PENNHEALTH.PRV> Content-Type: text/plain; charset="US-ASCII" Hello everyone, I am requesting information for a graduate student on how to fix and process alginate beads with embedded tissue culture cells for paraffin histology. The beads are fixed in 4% paraformaldehyde but seem to turn white and disintegrate during routine processing. Thanks very much, Keith Danielson, PhD Supervisor, IHC Lab Pennsylvania Hospital Philadelphia, PA 19107 Phone: 215 829-7726 or 829-3696 E-mail: keith.danielson@uphs.upenn.edu The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ------------------------------ Message: 3 Date: Fri, 1 Aug 2008 12:23:42 -0500 From: "Amber McKenzie" Subject: [Histonet] HT schools To: Message-ID: <03C921A1EAF7F541B16543F6EC6A4B3701D3AEBF@giamail2.Gia.com> Content-Type: text/plain; charset="US-ASCII" Where are all the HT accredited schools and why aren't there more out there? I've seen the online classes' people can take, but that requires them to be trained in a lab, as well, for the "hands on" part. So, actually the supervisor still has to train potential HT's "on the job" before they can sit for the board exam. Right? ------------------------------ Message: 4 Date: Fri, 1 Aug 2008 13:41:00 -0400 From: christopher.hayden@novartis.com Subject: [Histonet] Christopher Hayden is nowhere near the lab. To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 08/01/2008 and will not return until 08/11/2008. Good Day Everyone! I will be out of the lab from 2 August until 10 August. I'll be back at my desk on the morning of the 11th. I will have no access to corporate email during this time. If you have an EM-related matter, please contact the Lab Manager, Gregory Argentieri, at x2-8617 Otherwise, I'll get back to you on the 11th or shortly afterwards. Thanks! -CH ------------------------------ Message: 5 Date: Fri, 1 Aug 2008 14:13:13 -0400 From: "Victoria Baker" Subject: [Histonet] A question for BondMax users To: "Histo Net list server" Message-ID: <4f016b690808011113k4bceed66w3a76c8bb38fa8cb@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Happy Friday everyone! In the BondMax detection system there is a "post primary" step that contains 10% BSA in a Tris buffer. I've tested the protocol excluding this step and did not have any staining. I can't seem to get the answer I'm looking for from the company, so if anyone can help me I'd really appreciate it. Thanks Vikki ------------------------------ Message: 6 Date: Fri, 1 Aug 2008 14:18:43 -0400 From: "Victoria Baker" Subject: Re: [Histonet] HT schools To: "Amber McKenzie" Cc: histonet@lists.utsouthwestern.edu Message-ID: <4f016b690808011118k34ecf6c5r20c4960d00b679c6@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi Amber, If you go to the NSH website there are schools listed there. I'm an alumni of the SUNY school at Cobleskill - and I loved it - but it was also thirty years ago. I would check that list and see if you can't make some calls or e-mails to their co-ordinators. Regards, Vikki Baker On 8/1/08, Amber McKenzie wrote: > Where are all the HT accredited schools and why aren't there more out > there? I've seen the online classes' people can take, but that requires > them to be trained in a lab, as well, for the "hands on" part. So, > actually the supervisor still has to train potential HT's "on the job" > before they can sit for the board exam. Right? > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 7 Date: Fri, 1 Aug 2008 13:27:36 -0500 From: "Beckham, Sharon" Subject: RE: [Histonet] A question for BondMax users To: 'Victoria Baker' , Histo Net list server Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Vikki, I don't know if I'm much help on this because I just started testing the BondMax this week so I'm just in the learning process too. As far as I can tell the post primary is for blocking, but also works in conjunction with the polymer which is the following step. It supposedly also enhances penetration of the polymer, so they both have to be used together. Hope this helps. Sharon -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Friday, August 01, 2008 1:13 PM To: Histo Net list server Subject: [Histonet] A question for BondMax users Happy Friday everyone! In the BondMax detection system there is a "post primary" step that contains 10% BSA in a Tris buffer. I've tested the protocol excluding this step and did not have any staining. I can't seem to get the answer I'm looking for from the company, so if anyone can help me I'd really appreciate it. Thanks Vikki _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Fri, 1 Aug 2008 13:27:28 -0500 From: "Dana Brewer" Subject: [Histonet] Bone Marrow Biopsy Needles To: Message-ID: <000601c8f404$619b66c0$0b0a10ac@work.nmh.org> Content-Type: text/plain; charset="iso-8859-1" Does anyone have a replacement source for J Style Needle, -Marrow Loc- Bone Marrow Biopsy Needles -- no longer manufactured by US BIOPSY -- the replacement we got is not the same "marrow loc" system our oncologist prefers. Thanks for your help ------------------------------ Message: 9 Date: Fri, 1 Aug 2008 12:42:14 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] HT schools To: histonet@lists.utsouthwestern.edu, Amber McKenzie Message-ID: <78031.12752.qm@web65716.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Wrong! The advantage of the "on line" or "distance learning" courses is that they provide the theory on line while you are working at a given laboratory doing your training (or even as part of your daily work) so there is no "actual training" to be done by the supervisor. At this moment NAACLS has 30 HT and 3 HTL accredited programs only, with an overall capacity of about 300-325 students, and this will not be enough to take care of? all the retiring histotechs. Costs is one of the reasons why the number of HTs schools is dwindling. Ren? J. ? --- On Fri, 8/1/08, Amber McKenzie wrote: From: Amber McKenzie Subject: [Histonet] HT schools To: histonet@lists.utsouthwestern.edu Date: Friday, August 1, 2008, 1:23 PM Where are all the HT accredited schools and why aren't there more out there? I've seen the online classes' people can take, but that requires them to be trained in a lab, as well, for the "hands on" part. So, actually the supervisor still has to train potential HT's "on the job" before they can sit for the board exam. Right? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Fri, 1 Aug 2008 15:23:31 -0600 From: "Gayle Callis" Subject: [Histonet] processing PFA fixed alginate beads To: "Histonet Message" Message-ID: <000801c8f41c$d9196180$6401a8c0@Sunney> Content-Type: text/plain; charset="iso-8859-1" I Googled this subject and brought up several publications using alginate beads embedded in paraffin. HOwever, the following publication, using cryotomy, had better results than using paraffin sections. Also, Yang C-C, et al. Adapted cyrosectioning method for hydrogels used in regenerative medicine. J Histotechnology 30(3):185-191, 2007. Linda Jenkins, on of the co-authors, was a huge part of this, has much experience with hydrogels. In this publication, they fabricated their own alginate beads. The reference list is extensive and informative. Good Luck Gayle M. Callis HTL/HT/MT(ASCP) -------------------------------------------------------------------------------- ------------------------------ Message: 11 Date: Fri, 1 Aug 2008 15:28:53 -0600 From: "Gayle Callis" Subject: [Histonet] Methodology for extracting nucleic acids from FFPE Tissue To: "Histonet Message" Message-ID: <000f01c8f41d$990a30f0$6401a8c0@Sunney> Content-Type: text/plain; charset="iso-8859-1" For the person needing a protocol for extracting DNA and/or RNA from breast (?) tissue, check out QuickExtract FFPE DNA and RNA Extraction Kits from Epicenter Biotechnologies, www. EpiBio.com Phone # is 800 284-8474. This information was found in the latest issue of Science ( Life ScienCe Technologies section) Gayle M. Callis HTL/HT/MT(ASCP) ------------------------------ Message: 12 Date: Fri, 1 Aug 2008 18:14:56 -0400 From: "Michael Mihalik" Subject: RE: [Histonet] Dictation systems To: , Message-ID: <001501c8f424$204a2830$60de7890$@com> Content-Type: text/plain; charset="iso-8859-1" Jon, did you ever get an answer to this question? I am not as familiar with all the various dictation systems that I would like to be, but I have used something that may help. You can buy a foot pedal that can be 'programmed' to send a hotkey combination to an external device, typically a PC. In my case, I was considering using this setup to enable and disable Dragon Naturally Speaking at the gross workstation. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jon Google Sent: Tuesday, July 29, 2008 3:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dictation systems We are in the process of upgrading our LIS. We are looking at moving our dictation system from our current Dictaphone analog system. We would like to use a digital system that can support VOIP.? The problem we are running into is trying to find one that supports a hands free control that our grossing pathologists need. ? Does anyone have recommendations on digital systems that support foot controls? ? Thanks Jon _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Sat, 2 Aug 2008 07:19:36 -0500 From: "Joe Nocito" Subject: Re: [Histonet] HT schools To: , , "Amber McKenzie" Message-ID: <002c01c8f49a$07995790$e797b348@yourxhtr8hvc4p> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original our one community college program in San Antonio is in jeopardy of closing because it can't get the required 10 students to make a class. If this program does close, I will be willing to work with a college or university with long- distance learning. I have 5 openings in my lab. JTT ----- Original Message ----- From: "Rene J Buesa" To: ; "Amber McKenzie" Sent: Friday, August 01, 2008 2:42 PM Subject: Re: [Histonet] HT schools Wrong! The advantage of the "on line" or "distance learning" courses is that they provide the theory on line while you are working at a given laboratory doing your training (or even as part of your daily work) so there is no "actual training" to be done by the supervisor. At this moment NAACLS has 30 HT and 3 HTL accredited programs only, with an overall capacity of about 300-325 students, and this will not be enough to take care of all the retiring histotechs. Costs is one of the reasons why the number of HTs schools is dwindling. Ren? J. --- On Fri, 8/1/08, Amber McKenzie wrote: From: Amber McKenzie Subject: [Histonet] HT schools To: histonet@lists.utsouthwestern.edu Date: Friday, August 1, 2008, 1:23 PM Where are all the HT accredited schools and why aren't there more out there? I've seen the online classes' people can take, but that requires them to be trained in a lab, as well, for the "hands on" part. So, actually the supervisor still has to train potential HT's "on the job" before they can sit for the board exam. Right? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 57, Issue 2 *************************************** From cmiller <@t> physlab.com Mon Aug 4 08:05:24 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Mon Aug 4 08:06:35 2008 Subject: [Histonet] HT schools In-Reply-To: <78031.12752.qm@web65716.mail.ac4.yahoo.com> References: <03C921A1EAF7F541B16543F6EC6A4B3701D3AEBF@giamail2.Gia.com> <78031.12752.qm@web65716.mail.ac4.yahoo.com> Message-ID: <000d01c8f632$c218c4e0$3d02a8c0@plab.local> Someone has to teach them the "hands on" part of histology. I do not leave this to my staff. I teach / give them the skills they need to perform the practical part of their profession. As their supervisor I am very much involved in their training. I am sure I am not the only one.?? Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, August 01, 2008 2:42 PM To: histonet@lists.utsouthwestern.edu; Amber McKenzie Subject: Re: [Histonet] HT schools Wrong! The advantage of the "on line" or "distance learning" courses is that they provide the theory on line while you are working at a given laboratory doing your training (or even as part of your daily work) so there is no "actual training" to be done by the supervisor. At this moment NAACLS has 30 HT and 3 HTL accredited programs only, with an overall capacity of about 300-325 students, and this will not be enough to take care of? all the retiring histotechs. Costs is one of the reasons why the number of HTs schools is dwindling. Ren? J. ? --- On Fri, 8/1/08, Amber McKenzie wrote: From: Amber McKenzie Subject: [Histonet] HT schools To: histonet@lists.utsouthwestern.edu Date: Friday, August 1, 2008, 1:23 PM Where are all the HT accredited schools and why aren't there more out there? I've seen the online classes' people can take, but that requires them to be trained in a lab, as well, for the "hands on" part. So, actually the supervisor still has to train potential HT's "on the job" before they can sit for the board exam. Right? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From cmiller <@t> physlab.com Mon Aug 4 08:07:46 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Mon Aug 4 08:08:57 2008 Subject: [Histonet] surveillance cameras in the lab In-Reply-To: <080420081021.7972.4896D816000E927500001F242212020784CACC039D089B0EAF@comcast.net> References: <080420081021.7972.4896D816000E927500001F242212020784CACC039D089B0EAF@comcast.net> Message-ID: <000e01c8f633$16b9cee0$3d02a8c0@plab.local> That is B.S....I wouldn't work in a lab that used covalence camera's for what ever reason Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathrm35@comcast.net Sent: Monday, August 04, 2008 5:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] surveillance cameras in the lab I was wondering how many techs out there have cameras in their labs, either for security or to monitor employees. I went to work Sunday night and noticed that 4 cameras were installed in the lab over the weekend, with more to come. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From rfields <@t> gidocs.net Mon Aug 4 08:09:15 2008 From: rfields <@t> gidocs.net (Rosa Fields) Date: Mon Aug 4 08:09:22 2008 Subject: [Histonet] Dictation systems In-Reply-To: <015901c8f567$ef964b20$cec2e160$@com> References: <366247.75524.qm@web59902.mail.ac4.yahoo.com><001501c8f424$204a2830$60de7890$@com><982A0A9461F9BF438C7B19A6E425A383475B42@ITSSSXM01V6.one.ads.che.org> <015901c8f567$ef964b20$cec2e160$@com> Message-ID: <2F2611250DCD6549AA3D96CE8AF1F01801220644@giexchange.gidocs.net> We use Dragon at the gross station; although we don't have a foot pedal for it. We use microphone off, or go to sleep. I am pretty impressed with it. Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Sunday, August 03, 2008 7:54 AM To: 'Weems, Joyce'; ht.ascp@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dictation systems Can you explain what you mean when you say the 'headsets are voice activated'? Also, have you ever used Dragon before? There's a difference between 'go to sleep' and 'microphone off'. Where did you get the foot pedal from and how does it work, if you don't mind my asking? I'd be curious what other people's experience with Dragon is, if you can spare the time. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: Weems, Joyce [mailto:JWeems@sjha.org] Sent: Sunday, August 03, 2008 8:42 AM To: Michael Mihalik; ht.ascp@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dictation systems We are in the process of purchasing Dragon as we speak. The headsets are voice activated so I am counting on that working in the gross room, although I have included a foot pedal in the purchase request which will activate the head set. I will know more next week when it is installed. Let me know if you would like follow up and I'll keep you posted. I can't imagine yet how they will gross and edit at the same time... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Friday, August 01, 2008 6:15 PM To: ht.ascp@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dictation systems Jon, did you ever get an answer to this question? I am not as familiar with all the various dictation systems that I would like to be, but I have used something that may help. You can buy a foot pedal that can be 'programmed' to send a hotkey combination to an external device, typically a PC. In my case, I was considering using this setup to enable and disable Dragon Naturally Speaking at the gross workstation. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jon Google Sent: Tuesday, July 29, 2008 3:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dictation systems We are in the process of upgrading our LIS. We are looking at moving our dictation system from our current Dictaphone analog system. We would like to use a digital system that can support VOIP.? The problem we are running into is trying to find one that supports a hands free control that our grossing pathologists need. ? Does anyone have recommendations on digital systems that support foot controls? ? Thanks Jon _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Mon Aug 4 08:13:26 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Mon Aug 4 08:13:31 2008 Subject: [Histonet] surveillance cameras in the lab In-Reply-To: <080420081021.7972.4896D816000E927500001F242212020784CACC039D089B0EAF@comcast.net> References: <080420081021.7972.4896D816000E927500001F242212020784CACC039D089B0EAF@comcast.net> Message-ID: That's really creepy. You should stage a musical extravaganza for whomever watches it. Science: The Musical! Featuring the Busby Berkley rolling chair dance! PPE required (heigh-o!) Emily -- An overcivilized people grow complacent and careless and leave the door open for a tribe of fanatical savages, through a mixture of luck, treachery, and the foulest inhumanity, to usurp their place for a few years. -Richard Adams, "Shardik", 1974 From SBarnes <@t> elch.org Mon Aug 4 08:14:19 2008 From: SBarnes <@t> elch.org (Sue Barnes) Date: Mon Aug 4 08:17:11 2008 Subject: [Histonet] p63 and mammaglobin as RUO Message-ID: <807C106E9A171C46B0CF253C2507971E15AC58@elchex01.elch.net> How is everyone handling the P63 and Mammaglobin billing issue. Our pathologist went to a meeting where everyone was talking about these antibodies and how useful they were. We ordered them and he has been asking for them often. Just got a disclaimer from cell marque to sign saying that we realize these are for research only and cannot be billed. Does anyone have any information to share on the use of these antibodies. The pathologist is going to stop using them, even if they are useful, if he cannot bill for them. Thanks for any information Sue Barnes East Liverpool City Hospital East Liverpool, Ohio From mpence <@t> grhs.net Mon Aug 4 08:31:09 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Aug 4 08:31:15 2008 Subject: [Histonet] surveillance cameras in the lab In-Reply-To: <080420081021.7972.4896D816000E927500001F242212020784CACC039D089B0EAF@comcast.net> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A38C0@IS-E2K3.grhs.net> One way of finding out who is "watching you" is to blackout the lenses. This should get a quick response! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathrm35@comcast.net Sent: Monday, August 04, 2008 5:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] surveillance cameras in the lab I was wondering how many techs out there have cameras in their labs, either for security or to monitor employees. I went to work Sunday night and noticed that 4 cameras were installed in the lab over the weekend, with more to come. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rfields <@t> gidocs.net Mon Aug 4 08:46:02 2008 From: rfields <@t> gidocs.net (Rosa Fields) Date: Mon Aug 4 08:46:06 2008 Subject: [Histonet] surveillance cameras in the lab In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A38C0@IS-E2K3.grhs.net> References: <080420081021.7972.4896D816000E927500001F242212020784CACC039D089B0EAF@comcast.net> <661949901A768E4F9CC16D8AF8F2838C017A38C0@IS-E2K3.grhs.net> Message-ID: <2F2611250DCD6549AA3D96CE8AF1F0180122065D@giexchange.gidocs.net> You could put a nice coat of paraffin on the lenses! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Monday, August 04, 2008 8:31 AM To: Pathrm35@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] surveillance cameras in the lab One way of finding out who is "watching you" is to blackout the lenses. This should get a quick response! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathrm35@comcast.net Sent: Monday, August 04, 2008 5:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] surveillance cameras in the lab I was wondering how many techs out there have cameras in their labs, either for security or to monitor employees. I went to work Sunday night and noticed that 4 cameras were installed in the lab over the weekend, with more to come. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 <@t> earthlink.net Mon Aug 4 08:51:57 2008 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Aug 4 08:52:00 2008 Subject: [Histonet] RELIA Histology JOB ALERT!! Message-ID: Hi Histonetters! I hope you had a great weekend and are keeping cool during these dog days of summer!! I had a few really exciting new opportunities come in since the bulletin I posted last week. All of these are full time permanent positions offering excellent compensation, benefits and relocation assistance. Here are the positions and their locations: Histo Tech, Small private lab - Dallas, TX Histo Tech - Hospital Lab - El Paso, TX Histo Tech Mid-size private lab - San Francisco Bay area MOHS Tech - Williamsburg, VA Lab Supervisor Hospital Lab - El Paso, TX Histology Supervisor Prestigious Research and Clinical Facility - Buffalo, NY I also have histo tech positions in Los Angeles, Sacramento, Seattle, Spokane, Austin, Western MD, Southwest Florida and Southern VA. I also have management positions in Los Angeles, CA and Portland, OR,. If you or anyone else you know might be interested in any of these opportunities please contact me. I can be reached at relia1@earthlink.net or toll free at 866-607-3542. Thanks-Pam Thank You! Pam M. Barker President Relia 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll Free: (866)607-3542 e-mail: relia1@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia From Tiffany.Price <@t> thomaswv.org Mon Aug 4 08:56:37 2008 From: Tiffany.Price <@t> thomaswv.org (Price, Tiffany) Date: Mon Aug 4 08:56:57 2008 Subject: [Histonet] tissue falling out of paraffin block Message-ID: <7B5F5D9A00739F43A1819403EC7CF49108AA24FC@thm-mail.thomaswv.org> Can anyone tell me what would make liver tissue fall out of a paraffin block after routine processing? We cut a routine H&E slide, and later, had to cut IHC stains that were ordered. The tissue was fine for the H&E, but the whole tissue fell out of the block when we tried to re-cut it, even after re-embedding. Thanks for any help Tiffany Confidentiality Notice: This email message including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privilege information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy ALL copies of the message. From contact <@t> excaliburpathology.com Mon Aug 4 09:02:12 2008 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Mon Aug 4 09:02:15 2008 Subject: [Histonet] surveillance cameras in the lab Message-ID: <419055.10912.qm@web50106.mail.re2.yahoo.com> I thought about blacking out the lenses too. The worst part of this whole situation is that the cameras suddenly appeared.?The tech just happened to notice them. It is totally unacceptable to me that an institution would not inform the employees they were going to install cameras. This may be CYA for the powers that be. RUN! ?Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 631 N. Broadway Ave. Moore, OK 73160 405-570-6679 cell 405-759-3953 lab contact@excaliburpathology.com www.excaliburpathology.com From bill501 <@t> mindspring.com Mon Aug 4 09:02:27 2008 From: bill501 <@t> mindspring.com (Bill) Date: Mon Aug 4 09:02:46 2008 Subject: [Histonet] surveillance cameras in the lab In-Reply-To: <080420081021.7972.4896D816000E927500001F242212020784CACC039D089B0EAF@comc ast.net> References: <080420081021.7972.4896D816000E927500001F242212020784CACC039D089B0EAF@comc ast.net> Message-ID: At 10:21 AM +0000 8/4/08, Pathrm35@comcast.net wrote: >>>I was wondering how many techs out there have cameras in their labs, either for security or to monitor employees. I went to work Sunday night and noticed that 4 cameras were installed in the lab over the weekend, with more to come.<<< I would use immersion oil on the camera lenses. -- ______________ Bill Blank, MD Heartland Lab From contact <@t> excaliburpathology.com Mon Aug 4 09:03:53 2008 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Mon Aug 4 09:03:56 2008 Subject: [Histonet] surveillance cameras in the lab Message-ID: <499718.21489.qm@web50109.mail.re2.yahoo.com> Hydrofluoric acid etches glass ;) ?Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 631 N. Broadway Ave. Moore, OK 73160 405-570-6679 cell 405-759-3953 lab contact@excaliburpathology.com www.excaliburpathology.com ----- Original Message ---- From: Rosa Fields To: Mike Pence ; Pathrm35@comcast.net; histonet@lists.utsouthwestern.edu Sent: Monday, August 4, 2008 8:46:02 AM Subject: RE: [Histonet] surveillance cameras in the lab You could put a nice coat of paraffin on the lenses! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Monday, August 04, 2008 8:31 AM To: Pathrm35@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] surveillance cameras in the lab One way of finding out who is "watching you" is to blackout the lenses. This should get a quick response! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathrm35@comcast.net Sent: Monday, August 04, 2008 5:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] surveillance cameras in the lab I was wondering how many techs out there have cameras in their labs, either for security or to monitor employees. I went to work Sunday night and noticed that 4 cameras were installed in the lab over the weekend, with more to come. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Mon Aug 4 09:17:38 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Mon Aug 4 09:17:53 2008 Subject: [Histonet] surveillance cameras in the lab In-Reply-To: <419055.10912.qm@web50106.mail.re2.yahoo.com> References: <419055.10912.qm@web50106.mail.re2.yahoo.com> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A7F23E22@LTA3VS011.ees.hhs.gov> I am not against the idea of the cameras...but I would definitely have an issue with no one being notified that they were going to be installed and why. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Monday, August 04, 2008 10:02 AM To: Histonet Subject: [Histonet] surveillance cameras in the lab I thought about blacking out the lenses too. The worst part of this whole situation is that the cameras suddenly appeared.?The tech just happened to notice them. It is totally unacceptable to me that an institution would not inform the employees they were going to install cameras. This may be CYA for the powers that be. RUN! ?Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 631 N. Broadway Ave. Moore, OK 73160 405-570-6679 cell 405-759-3953 lab contact@excaliburpathology.com www.excaliburpathology.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Weber2 <@t> va.gov Mon Aug 4 09:34:05 2008 From: Susan.Weber2 <@t> va.gov (Weber, Susan (VHACLE)) Date: Mon Aug 4 09:34:41 2008 Subject: [Histonet] surveillance cameras in the lab In-Reply-To: <1CE1847DFEA0A647B1CCDE4108EA60A7F23E22@LTA3VS011.ees.hhs.gov> References: <419055.10912.qm@web50106.mail.re2.yahoo.com> <1CE1847DFEA0A647B1CCDE4108EA60A7F23E22@LTA3VS011.ees.hhs.gov> Message-ID: <16C83872A53F4346AA9C3A18E3A3AAB903F76E15@VHAV10MSGA1.v10.med.va.gov> Was this through out the whole lab, or just Histology. The other departments may be interested in knowing they are being "observed" dare I say "watched", "spied upon", etc. It is highly unacceptable to me that there is no notification to the "workers" that big bro is hovering overhead. Also unacceptable is the fact that "they" are not giving a very good "reason" for the install. BTW- wear proper PPE so they can't tell who is "cleaning" that lens with the nice immersion oil. Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Monday, August 04, 2008 10:18 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] surveillance cameras in the lab I am not against the idea of the cameras...but I would definitely have an issue with no one being notified that they were going to be installed and why. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Monday, August 04, 2008 10:02 AM To: Histonet Subject: [Histonet] surveillance cameras in the lab I thought about blacking out the lenses too. The worst part of this whole situation is that the cameras suddenly appeared.?The tech just happened to notice them. It is totally unacceptable to me that an institution would not inform the employees they were going to install cameras. This may be CYA for the powers that be. RUN! ?Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 631 N. Broadway Ave. Moore, OK 73160 405-570-6679 cell 405-759-3953 lab contact@excaliburpathology.com www.excaliburpathology.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gmartin <@t> marshallmedical.org Mon Aug 4 09:42:47 2008 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Mon Aug 4 09:43:00 2008 Subject: [Histonet] surveillance cameras in the lab In-Reply-To: <419055.10912.qm@web50106.mail.re2.yahoo.com> References: <419055.10912.qm@web50106.mail.re2.yahoo.com> Message-ID: <6ED9D4252F278841A0593D3D788AF24C02FC5A1F@mailsvr.MARSHMED.local> The camera thing is odd! There should be some sort of written policy to accompany this process. I was a school board member for several years and the other members insisted on installing cameras in the buss'. This, as one responder mentioned, caused all kinds of unanticipated problems. The first and foremost was that all information gathered was after the fact and the images were not clear enough to assign blame. Also it was surprising who got their hand on those recordings for their viewing pleasure. Needless to say the very expensive cameras were removed after two lawsuits by parents. I do agree with one of the responders ... that this is something the administration is not handling directly. They at least owe you an explanation! Gary -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Monday, August 04, 2008 7:02 AM To: Histonet Subject: [Histonet] surveillance cameras in the lab I thought about blacking out the lenses too. The worst part of this whole situation is that the cameras suddenly appeared.?The tech just happened to notice them. It is totally unacceptable to me that an institution would not inform the employees they were going to install cameras. This may be CYA for the powers that be. RUN! ?Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 631 N. Broadway Ave. Moore, OK 73160 405-570-6679 cell 405-759-3953 lab contact@excaliburpathology.com www.excaliburpathology.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Mon Aug 4 09:45:40 2008 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Mon Aug 4 09:45:45 2008 Subject: [Histonet] tissue falling out of paraffin block In-Reply-To: <7B5F5D9A00739F43A1819403EC7CF49108AA24FC@thm-mail.thomaswv.org> Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835C45@LSRIEXCH1.lsmaster.lifespan.org> That sounds like incomplete dehydration. A small amount of water remains in the tissue. This prevents full infiltration by both the clearling agent and the paraffin. Once the block is faced off, the water and/or solvent evaporates from the tissue, causing it to shrink and pull away from the paraffin. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Price, Tiffany > Sent: Monday, August 4, 2008 9:56 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] tissue falling out of paraffin block > > Can anyone tell me what would make liver tissue fall out of a paraffin > block after routine processing? We cut a routine H&E slide, and later, > had to cut IHC stains that were ordered. The tissue was fine for the > H&E, but the whole tissue fell out of the block when we tried to re-cut > it, even after re-embedding. > > Thanks for any help > Tiffany > Confidentiality Notice: This email message including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privilege information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy ALL copies of the message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From dusko.trajkovic <@t> pfizer.com Mon Aug 4 09:49:29 2008 From: dusko.trajkovic <@t> pfizer.com (Trajkovic, Dusko) Date: Mon Aug 4 09:49:19 2008 Subject: [Histonet] PTAH staining on Carnoy's fixed tissue In-Reply-To: <86F334797DC6524A99AD9DD8F23A8B50111A61@mailserv.hrsu.mrc.ac.uk> Message-ID: <3AD0BD3142459B4E9B12CBEAFF2B89B207AC423B@lajamrexm01.amer.pfizer.com> Good Morning/Day/Evening Histonet colleagues, Does anyone have a protocol for staining PTAH on Carnoy's fixed tissue? How does the fixative impact the PTAH staining? Any information would be greatly appreciated. Thank you, Dusko Trajkovic From Sharon.Davis-Devine <@t> carle.com Mon Aug 4 09:59:33 2008 From: Sharon.Davis-Devine <@t> carle.com (Sharon.Davis-Devine) Date: Mon Aug 4 09:59:38 2008 Subject: [Histonet] p16 on Ventana Message-ID: <44780C571F28624DBB446DE55C4D733A021E0A51@EXCHANGEBE1.carle.com> Hey Histonetters! Is anyone out there in histoworld running p16 on the Ventana system? If so, can you provide us with your protocol and where you are ordering it from? We are aware that MTM is presently the only vendor for p16 but it is rather pricey and are wondering if there are any alternatives available out there. Once again thank you so much for your help. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com From kenneth.a.troutman <@t> Vanderbilt.Edu Mon Aug 4 11:03:17 2008 From: kenneth.a.troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Mon Aug 4 11:03:23 2008 Subject: [Histonet] surveillance cameras in the lab Message-ID: <37DEF9AF72994947AF693956A59B9B660127FF52@mailbe03.mc.vanderbilt.edu> I used to work in a lab that had surveillance cameras. At the time, we only had one that was over our single grossing station to monitor grossing. (There were some missing tissue accusations that needed to be nipped in the bud, so to say...) I eventually started managing the accessioning department and we installed 4 to monitor specimen receipt to see if anything was accidently thrown in the trash. It was supposed to be a quick reference tool that was designed to keep us from digging in the biohazard trash unnecessarily, however, it proved to be more useful for catching employees napping! (Yes, they were all aware that the cameras existed!) As for cameras in the lab in general, you will probably have to ask upper management (or whoever made the decision to install them) why they are there. Perhaps they may even tell you the answer. Good luck. Ashley Troutman BS, HT(ASCP)QIHC Histopathology Laboratory Department of Pathology Vanderbilt University Medical Center Nashville, TN From amber.mckenzie <@t> gastrodocs.net Mon Aug 4 11:06:26 2008 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Mon Aug 4 11:06:31 2008 Subject: [Histonet] stainless steel bucket Message-ID: <03C921A1EAF7F541B16543F6EC6A4B3701D3B09F@giamail2.Gia.com> Does anyone know where I can purchase a stainless steel basket/bucket to wash my cassette lids in? We use xylene and Liqui-Nox to wash them each day and our plastic buckets eventually end up tearing and breaking over time and I have to constantly replace them, so I'm looking for something more stable like stainless steel. From pruegg <@t> ihctech.net Mon Aug 4 11:07:15 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Aug 4 11:07:01 2008 Subject: [Histonet] p16 on Ventana In-Reply-To: <44780C571F28624DBB446DE55C4D733A021E0A51@EXCHANGEBE1.carle.com> Message-ID: Sharon, The NSH IHC Resource Group just had a discussion about p16 and some alternatives were offered. NSH members can join the ihcrg online at www.ihcrg.org Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Monday, August 04, 2008 9:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 on Ventana Hey Histonetters! Is anyone out there in histoworld running p16 on the Ventana system? If so, can you provide us with your protocol and where you are ordering it from? We are aware that MTM is presently the only vendor for p16 but it is rather pricey and are wondering if there are any alternatives available out there. Once again thank you so much for your help. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tissuearray <@t> hotmail.com Mon Aug 4 11:12:27 2008 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Mon Aug 4 11:12:32 2008 Subject: [Histonet] Tissue Microarray Location Markers Message-ID: I am trying to find out what the best way is to determine the number one spot on a TMA? We use died lung for location markers. One color punch represents a group of punches. What other ways are techs marking their TMA locations? I know there are a variety of ways for documentation of TMAs but I am interested in the TMA block and location marker techniques and materials. Thanks all, Thom HT (ASAP) TMA Technician New TMA instrument go to: arraymold.com _________________________________________________________________ Your PC, mobile phone, and online services work together like never before. http://clk.atdmt.com/MRT/go/108587394/direct/01/ From pruegg <@t> ihctech.net Mon Aug 4 11:15:57 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Aug 4 11:15:40 2008 Subject: [Histonet] surveillance cameras in the lab In-Reply-To: Message-ID: As an employer I am unaware of any regulations requiring you to inform employees that you will install cameras for security or any other reason, you would probably need to post a sign stating that there are cameras being used in the area is all? Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bill Sent: Monday, August 04, 2008 8:02 AM To: Pathrm35@comcast.net; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] surveillance cameras in the lab At 10:21 AM +0000 8/4/08, Pathrm35@comcast.net wrote: >>>I was wondering how many techs out there have cameras in their labs, either for security or to monitor employees. I went to work Sunday night and noticed that 4 cameras were installed in the lab over the weekend, with more to come.<<< I would use immersion oil on the camera lenses. -- ______________ Bill Blank, MD Heartland Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Mon Aug 4 11:20:35 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Aug 4 11:20:17 2008 Subject: [Histonet] tissue falling out of paraffin block In-Reply-To: <7B5F5D9A00739F43A1819403EC7CF49108AA24FC@thm-mail.thomaswv.org> Message-ID: I see this all the time with animal tissue, I think it is in samples that are particularly bloody after soaking in the ice water bath before cutting, it is like the paraffin does not infiltrate the tissue very well and it is separate from the surrounding paraffin in the block. Usually reembedding does the trick for me. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Price, Tiffany Sent: Monday, August 04, 2008 7:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tissue falling out of paraffin block Can anyone tell me what would make liver tissue fall out of a paraffin block after routine processing? We cut a routine H&E slide, and later, had to cut IHC stains that were ordered. The tissue was fine for the H&E, but the whole tissue fell out of the block when we tried to re-cut it, even after re-embedding. Thanks for any help Tiffany Confidentiality Notice: This email message including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privilege information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy ALL copies of the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Mon Aug 4 11:23:03 2008 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Mon Aug 4 11:25:06 2008 Subject: [Histonet] stainless steel bucket References: <03C921A1EAF7F541B16543F6EC6A4B3701D3B09F@giamail2.Gia.com> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2748@fhosxchmb006.ADVENTISTCORP.NET> Ask your surgery Department for a catalog or maybe they have some old pieces they'd be happy to let you have! If you're a Reference Lab with no Surgery, Mopec carries a nice line of Pathology Morgue Equipment. Janet Janet L. Bonner, HTL (ASCP) Pathology Laboratory Florida Hospital Winter Park janet.bonner@FLHOSP.org 407-646-7559 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Amber McKenzie Sent: Mon 8/4/2008 12:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] stainless steel bucket Does anyone know where I can purchase a stainless steel basket/bucket to wash my cassette lids in? We use xylene and Liqui-Nox to wash them each day and our plastic buckets eventually end up tearing and breaking over time and I have to constantly replace them, so I'm looking for something more stable like stainless steel. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From ruebenjcarter <@t> gmail.com Mon Aug 4 11:25:07 2008 From: ruebenjcarter <@t> gmail.com (R C) Date: Mon Aug 4 11:25:16 2008 Subject: [Histonet] Re: Rabbit IgG control Message-ID: <2a926e3f0808040925s38e922f4mda2156f97b5592d9@mail.gmail.com> Jackson Immuno Research. On Sat, Aug 2, 2008 at 10:01 AM, wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Rabbit IgG control (Margaryan, Naira) > 2. Alginate beads containing cells (Danielson, Keith) > 3. HT schools (Amber McKenzie) > 4. Christopher Hayden is nowhere near the lab. > (christopher.hayden@novartis.com) > 5. A question for BondMax users (Victoria Baker) > 6. Re: HT schools (Victoria Baker) > 7. RE: A question for BondMax users (Beckham, Sharon) > 8. Bone Marrow Biopsy Needles (Dana Brewer) > 9. Re: HT schools (Rene J Buesa) > 10. processing PFA fixed alginate beads (Gayle Callis) > 11. Methodology for extracting nucleic acids from FFPE Tissue > (Gayle Callis) > 12. RE: Dictation systems (Michael Mihalik) > 13. Re: HT schools (Joe Nocito) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 1 Aug 2008 12:03:09 -0500 > From: "Margaryan, Naira" > Subject: [Histonet] Rabbit IgG control > To: > Message-ID: > < > EE5AF506236CEE4CAFD1A281F5DCFE90021035C8@CMHEXC02EVS.childrensmemorial.org > > > > Content-Type: text/plain; charset="us-ascii" > > Hi Dears, > > > > I have to order a Rabbit IgG control. What company is making best Rabbit > IgG control, not universal, please. > > > > Thanks in advance, > > Naira > > > > Naira V. Margaryan, D.V.M., Ph.D. > > Research Scientist > > Children's Memorial Research Center > > 2300 Children's Plaza, Box 222 > > Chicago, IL 60614-3363 > > Tel: 773-755-6340 > > Fax: 773-755-6594 > > nmargaryan@childrensmemorial.org > > > > > For Express Mail: > > CMRC, Room C.473 > > 2430 N. Halsted Street > > Chicago, IL 60614-4314 > > > > > > ------------------------------ > > Message: 2 > Date: Fri, 1 Aug 2008 13:07:21 -0400 > From: "Danielson, Keith" > Subject: [Histonet] Alginate beads containing cells > To: > Message-ID: > > <84D11048BB18234D8A56474645E8EC020829EF6F@uphsmbx5.UPHS.PENNHEALTH.PRV> > > Content-Type: text/plain; charset="US-ASCII" > > Hello everyone, > > > > I am requesting information for a graduate student on how to fix and > process alginate beads with embedded tissue culture cells for paraffin > histology. The beads are fixed in 4% paraformaldehyde but seem to turn > white and disintegrate during routine processing. > > > > Thanks very much, > > > > Keith Danielson, PhD > > Supervisor, IHC Lab > > Pennsylvania Hospital > > Philadelphia, PA 19107 > > > > Phone: 215 829-7726 or 829-3696 > > E-mail: keith.danielson@uphs.upenn.edu > > > > > > > > The information contained in this e-mail message is intended only for the > personal and confidential use of the recipient(s) named above. If the reader > of this message is not the intended recipient or an agent responsible for > delivering it to the intended recipient, you are hereby notified that you > have received this document in error and that any review, dissemination, > distribution, or copying of this message is strictly prohibited. If you have > received this communication in error, please notify us immediately by > e-mail, and delete the original message. > > ------------------------------ > > Message: 3 > Date: Fri, 1 Aug 2008 12:23:42 -0500 > From: "Amber McKenzie" > Subject: [Histonet] HT schools > To: > Message-ID: > <03C921A1EAF7F541B16543F6EC6A4B3701D3AEBF@giamail2.Gia.com> > Content-Type: text/plain; charset="US-ASCII" > > Where are all the HT accredited schools and why aren't there more out > there? I've seen the online classes' people can take, but that requires > them to be trained in a lab, as well, for the "hands on" part. So, > actually the supervisor still has to train potential HT's "on the job" > before they can sit for the board exam. Right? > > > > > > > > ------------------------------ > > Message: 4 > Date: Fri, 1 Aug 2008 13:41:00 -0400 > From: christopher.hayden@novartis.com > Subject: [Histonet] Christopher Hayden is nowhere near the lab. > To: histonet@lists.utsouthwestern.edu > Message-ID: > < > OFC6859B0A.C0BE57E7-ON85257498.0061233C-85257498.0061233C@ah.novartis.com> > > Content-Type: text/plain; charset=US-ASCII > > > I will be out of the office starting 08/01/2008 and will not return until > 08/11/2008. > > Good Day Everyone! > > I will be out of the lab from 2 August until 10 August. I'll be back at my > desk on the morning of the 11th. I will have no access to corporate email > during this time. > > If you have an EM-related matter, please contact the Lab Manager, Gregory > Argentieri, at x2-8617 > > Otherwise, I'll get back to you on the 11th or shortly afterwards. > > Thanks! > -CH > > > > > ------------------------------ > > Message: 5 > Date: Fri, 1 Aug 2008 14:13:13 -0400 > From: "Victoria Baker" > Subject: [Histonet] A question for BondMax users > To: "Histo Net list server" > Message-ID: > <4f016b690808011113k4bceed66w3a76c8bb38fa8cb@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Happy Friday everyone! > > In the BondMax detection system there is a "post primary" step that > contains 10% BSA in a Tris buffer. I've tested the protocol excluding > this step and did not have any staining. > > I can't seem to get the answer I'm looking for from the company, so if > anyone can help me I'd really appreciate it. > > Thanks > > Vikki > > > > ------------------------------ > > Message: 6 > Date: Fri, 1 Aug 2008 14:18:43 -0400 > From: "Victoria Baker" > Subject: Re: [Histonet] HT schools > To: "Amber McKenzie" > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > <4f016b690808011118k34ecf6c5r20c4960d00b679c6@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Hi Amber, > > If you go to the NSH website there are schools listed there. I'm an > alumni of the SUNY school at Cobleskill - and I loved it - but it was > also thirty years ago. > > I would check that list and see if you can't make some calls or > e-mails to their co-ordinators. > > Regards, > > Vikki Baker > > > > On 8/1/08, Amber McKenzie wrote: > > Where are all the HT accredited schools and why aren't there more out > > there? I've seen the online classes' people can take, but that requires > > them to be trained in a lab, as well, for the "hands on" part. So, > > actually the supervisor still has to train potential HT's "on the job" > > before they can sit for the board exam. Right? > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 7 > Date: Fri, 1 Aug 2008 13:27:36 -0500 > From: "Beckham, Sharon" > Subject: RE: [Histonet] A question for BondMax users > To: 'Victoria Baker' , Histo Net list server > > Message-ID: > < > BD62CBAC4395B94096109020651BE2EC129EDDCE2F@exchmb-02.stowers-institute.org > > > > Content-Type: text/plain; charset="us-ascii" > > Hi Vikki, > > I don't know if I'm much help on this because I just started testing the > BondMax this week so I'm just in the learning process too. As far as I can > tell the post primary is for blocking, but also works in conjunction with > the polymer which is the following step. It supposedly also enhances > penetration of the polymer, so they both have to be used together. Hope > this helps. > > Sharon > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Baker > Sent: Friday, August 01, 2008 1:13 PM > To: Histo Net list server > Subject: [Histonet] A question for BondMax users > > > Happy Friday everyone! > > In the BondMax detection system there is a "post primary" step that > contains 10% BSA in a Tris buffer. I've tested the protocol excluding this > step and did not have any staining. > > I can't seem to get the answer I'm looking for from the company, so if > anyone can help me I'd really appreciate it. > > Thanks > > Vikki > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 8 > Date: Fri, 1 Aug 2008 13:27:28 -0500 > From: "Dana Brewer" > Subject: [Histonet] Bone Marrow Biopsy Needles > To: > Message-ID: <000601c8f404$619b66c0$0b0a10ac@work.nmh.org> > Content-Type: text/plain; charset="iso-8859-1" > > Does anyone have a replacement source for J Style Needle, -Marrow Loc- Bone > Marrow Biopsy Needles -- no longer manufactured by US BIOPSY -- the > replacement we got is not the same "marrow loc" system our oncologist > prefers. > Thanks for your help > > ------------------------------ > > Message: 9 > Date: Fri, 1 Aug 2008 12:42:14 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] HT schools > To: histonet@lists.utsouthwestern.edu, Amber McKenzie > > Message-ID: <78031.12752.qm@web65716.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Wrong! The advantage of the "on line" or "distance learning" courses is > that they provide the theory on line while you are working at a given > laboratory doing your training (or even as part of your daily work) so there > is no "actual training" to be done by the supervisor. > At this moment NAACLS has 30 HT and 3 HTL accredited programs only, with an > overall capacity of about 300-325 students, and this will not be enough to > take care of all the retiring histotechs. > Costs is one of the reasons why the number of HTs schools is dwindling. > Ren? J. > > > --- On Fri, 8/1/08, Amber McKenzie wrote: > > From: Amber McKenzie > Subject: [Histonet] HT schools > To: histonet@lists.utsouthwestern.edu > Date: Friday, August 1, 2008, 1:23 PM > > Where are all the HT accredited schools and why aren't there more out > there? I've seen the online classes' people can take, but that > requires > them to be trained in a lab, as well, for the "hands on" part. So, > actually the supervisor still has to train potential HT's "on the > job" > before they can sit for the board exam. Right? > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 10 > Date: Fri, 1 Aug 2008 15:23:31 -0600 > From: "Gayle Callis" > Subject: [Histonet] processing PFA fixed alginate beads > To: "Histonet Message" > Message-ID: <000801c8f41c$d9196180$6401a8c0@Sunney> > Content-Type: text/plain; charset="iso-8859-1" > > I Googled this subject and brought up several publications using alginate > beads embedded in paraffin. HOwever, the following publication, using > cryotomy, had better results than using paraffin sections. > > Also, Yang C-C, et al. Adapted cyrosectioning method for hydrogels used in > regenerative medicine. J Histotechnology 30(3):185-191, 2007. Linda > Jenkins, on of the co-authors, was a huge part of this, has much experience > with hydrogels. In this publication, they fabricated their own alginate > beads. The reference list is extensive and informative. > > Good Luck > > Gayle M. Callis > HTL/HT/MT(ASCP) > > > > -------------------------------------------------------------------------------- > > > > > > > ------------------------------ > > Message: 11 > Date: Fri, 1 Aug 2008 15:28:53 -0600 > From: "Gayle Callis" > Subject: [Histonet] Methodology for extracting nucleic acids from FFPE > Tissue > To: "Histonet Message" > Message-ID: <000f01c8f41d$990a30f0$6401a8c0@Sunney> > Content-Type: text/plain; charset="iso-8859-1" > > For the person needing a protocol for extracting DNA and/or RNA from breast > (?) tissue, check out QuickExtract FFPE DNA and RNA Extraction Kits from > Epicenter Biotechnologies, www. EpiBio.com > > Phone # is 800 284-8474. > > This information was found in the latest issue of Science ( Life ScienCe > Technologies section) > > Gayle M. Callis > HTL/HT/MT(ASCP) > > > > ------------------------------ > > Message: 12 > Date: Fri, 1 Aug 2008 18:14:56 -0400 > From: "Michael Mihalik" > Subject: RE: [Histonet] Dictation systems > To: , > Message-ID: <001501c8f424$204a2830$60de7890$@com> > Content-Type: text/plain; charset="iso-8859-1" > > Jon, did you ever get an answer to this question? > > I am not as familiar with all the various dictation systems that I would > like to be, but I have used something that may help. > > You can buy a foot pedal that can be 'programmed' to send a hotkey > combination to an external device, typically a PC. > > In my case, I was considering using this setup to enable and disable > Dragon > Naturally Speaking at the gross workstation. > > Michael Mihalik > PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jon Google > Sent: Tuesday, July 29, 2008 3:29 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Dictation systems > > We are in the process of upgrading our LIS. We are looking at moving our > dictation system from our current Dictaphone analog system. We would like > to > use a digital system that can support VOIP. The problem we are running > into > is trying to find one that supports a hands free control that our grossing > pathologists need. > > Does anyone have recommendations on digital systems that support foot > controls? > > Thanks > Jon > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 13 > Date: Sat, 2 Aug 2008 07:19:36 -0500 > From: "Joe Nocito" > Subject: Re: [Histonet] HT schools > To: , , "Amber > McKenzie" > Message-ID: <002c01c8f49a$07995790$e797b348@yourxhtr8hvc4p> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > our one community college program in San Antonio is in jeopardy of closing > because it can't get the required 10 students to make a class. If this > program does close, I will be willing to work with a college or university > with long- distance learning. I have 5 openings in my lab. > > JTT > ----- Original Message ----- > From: "Rene J Buesa" > To: ; "Amber McKenzie" > > Sent: Friday, August 01, 2008 2:42 PM > Subject: Re: [Histonet] HT schools > > > Wrong! The advantage of the "on line" or "distance learning" courses is > that > they provide the theory on line while you are working at a given laboratory > doing your training (or even as part of your daily work) so there is no > "actual training" to be done by the supervisor. > At this moment NAACLS has 30 HT and 3 HTL accredited programs only, with an > overall capacity of about 300-325 students, and this will not be enough to > take care of all the retiring histotechs. > Costs is one of the reasons why the number of HTs schools is dwindling. > Ren? J. > > > --- On Fri, 8/1/08, Amber McKenzie wrote: > > From: Amber McKenzie > Subject: [Histonet] HT schools > To: histonet@lists.utsouthwestern.edu > Date: Friday, August 1, 2008, 1:23 PM > > Where are all the HT accredited schools and why aren't there more out > there? I've seen the online classes' people can take, but that > requires > them to be trained in a lab, as well, for the "hands on" part. So, > actually the supervisor still has to train potential HT's "on the > job" > before they can sit for the board exam. Right? > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 57, Issue 2 > *************************************** > From pruegg <@t> ihctech.net Mon Aug 4 11:46:26 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Aug 4 11:46:12 2008 Subject: [Histonet] HT schools In-Reply-To: <000d01c8f632$c218c4e0$3d02a8c0@plab.local> Message-ID: Remember that now there is no practical portion of the HT exam, so they are not being tested on hands on experiences anyway. The most difficult problem I have with training people on the job (and I have trained many) is that now they are not prepared to take the exam because they are examined all on theory. I have some really well trained people who can do the work really well, but they have a hard time taking the computer test which they pretty much have to memorize out of books. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Monday, August 04, 2008 7:05 AM To: rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; 'Amber McKenzie' Subject: RE: [Histonet] HT schools Someone has to teach them the "hands on" part of histology. I do not leave this to my staff. I teach / give them the skills they need to perform the practical part of their profession. As their supervisor I am very much involved in their training. I am sure I am not the only one.?? Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, August 01, 2008 2:42 PM To: histonet@lists.utsouthwestern.edu; Amber McKenzie Subject: Re: [Histonet] HT schools Wrong! The advantage of the "on line" or "distance learning" courses is that they provide the theory on line while you are working at a given laboratory doing your training (or even as part of your daily work) so there is no "actual training" to be done by the supervisor. At this moment NAACLS has 30 HT and 3 HTL accredited programs only, with an overall capacity of about 300-325 students, and this will not be enough to take care of? all the retiring histotechs. Costs is one of the reasons why the number of HTs schools is dwindling. Ren? J. ? --- On Fri, 8/1/08, Amber McKenzie wrote: From: Amber McKenzie Subject: [Histonet] HT schools To: histonet@lists.utsouthwestern.edu Date: Friday, August 1, 2008, 1:23 PM Where are all the HT accredited schools and why aren't there more out there? I've seen the online classes' people can take, but that requires them to be trained in a lab, as well, for the "hands on" part. So, actually the supervisor still has to train potential HT's "on the job" before they can sit for the board exam. Right? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Mon Aug 4 11:46:26 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Aug 4 11:46:15 2008 Subject: [Histonet] surveillance cameras in the lab In-Reply-To: <000e01c8f633$16b9cee0$3d02a8c0@plab.local> Message-ID: Come on. In these times of terror concerns I am not sure I would work in a place where I did not feel secure and the use of these devices help in that matter. We built a brand new University of Colorado Health Sciences Center and there are cameras all over the place as well as lock down. If you do not have an access card you cannot get into the labs. This was a pain at first but with all the crazy's we have to worry about out there it now makes me feel better. I just read in the paper this morning about a researcher whose house was bombed by Peta types for doing animal research, and we have had all sorts of disturbances over the years with precious research animals being released, protests, etc. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Monday, August 04, 2008 7:08 AM To: Pathrm35@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] surveillance cameras in the lab That is B.S....I wouldn't work in a lab that used covalence camera's for what ever reason Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathrm35@comcast.net Sent: Monday, August 04, 2008 5:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] surveillance cameras in the lab I was wondering how many techs out there have cameras in their labs, either for security or to monitor employees. I went to work Sunday night and noticed that 4 cameras were installed in the lab over the weekend, with more to come. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From will <@t> histologytechservices.com Mon Aug 4 11:58:44 2008 From: will <@t> histologytechservices.com (William Connor) Date: Mon Aug 4 11:59:06 2008 Subject: [Histonet] QIHC Certification Message-ID: <0MKpCa-1KQ3OY3GeG-0004hL@mrelay.perfora.net> Greetings, I have just sent in my application in for the QIHC exam. Would anyone out there kindly recommend to me the best study materials to focus on? I have Carson's book and Dako's Immunohistochemical Staining Methods, and am considering the purchase of Diagnostic Immunohistochemistry by Dabbs. I do not work in a hospital setting, but in more of a research setting. Would there be anything I might be missing out on in day to day practice which might be covered on the exam? Thank you in advance for your responses. William P. Connor, HT(ASCP) Senior Histology Technician Histology Tech Services, Inc. This email and its attachments (if any) contain confidential and privileged information. Any dissemination or use of this information by a person other than the intended recipient is unauthorized and may be illegal. From amber.mckenzie <@t> gastrodocs.net Mon Aug 4 12:08:53 2008 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Mon Aug 4 12:09:03 2008 Subject: [Histonet] HT schools In-Reply-To: <20080804164610.8611613F8@mail.gastrodocs.net> Message-ID: <03C921A1EAF7F541B16543F6EC6A4B3701D3B0C3@giamail2.Gia.com> Even if the online schools teach the theory of Histology, they expect the supervisor/techs to teach the potential HT's how to perform Histology. Even though there is no practical anymore, potential HT's have to be taught the hands on part of the job with OJT. Even though I've heard many people say that there is no more OJT, really there is b/c if these future HT's don't attend an actual HT school where there are classrooms/practice labs full of teachers, then we as working HT's have to teach our future co-workers how to do our job so that we'll have more people to pick from to hire. Does any other profession handle their future employee's like this or is Histology in a category of its own? -----Original Message----- From: Patsy Ruegg [mailto:pruegg@ihctech.net] Sent: Monday, August 04, 2008 11:46 AM To: 'Cheri Miller'; rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; Amber McKenzie Subject: RE: [Histonet] HT schools Remember that now there is no practical portion of the HT exam, so they are not being tested on hands on experiences anyway. The most difficult problem I have with training people on the job (and I have trained many) is that now they are not prepared to take the exam because they are examined all on theory. I have some really well trained people who can do the work really well, but they have a hard time taking the computer test which they pretty much have to memorize out of books. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Monday, August 04, 2008 7:05 AM To: rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; 'Amber McKenzie' Subject: RE: [Histonet] HT schools Someone has to teach them the "hands on" part of histology. I do not leave this to my staff. I teach / give them the skills they need to perform the practical part of their profession. As their supervisor I am very much involved in their training. I am sure I am not the only one.?? Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, August 01, 2008 2:42 PM To: histonet@lists.utsouthwestern.edu; Amber McKenzie Subject: Re: [Histonet] HT schools Wrong! The advantage of the "on line" or "distance learning" courses is that they provide the theory on line while you are working at a given laboratory doing your training (or even as part of your daily work) so there is no "actual training" to be done by the supervisor. At this moment NAACLS has 30 HT and 3 HTL accredited programs only, with an overall capacity of about 300-325 students, and this will not be enough to take care of all the retiring histotechs. Costs is one of the reasons why the number of HTs schools is dwindling. Ren? J. --- On Fri, 8/1/08, Amber McKenzie wrote: From: Amber McKenzie Subject: [Histonet] HT schools To: histonet@lists.utsouthwestern.edu Date: Friday, August 1, 2008, 1:23 PM Where are all the HT accredited schools and why aren't there more out there? I've seen the online classes' people can take, but that requires them to be trained in a lab, as well, for the "hands on" part. So, actually the supervisor still has to train potential HT's "on the job" before they can sit for the board exam. Right? From Jackie.O'Connor <@t> abbott.com Mon Aug 4 12:13:18 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Mon Aug 4 12:13:39 2008 Subject: [Histonet] stainless steel bucket In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B3701D3B09F@giamail2.Gia.com> Message-ID: Dog supply store. I purchase stainless steel water buckets for my kennel - they even have handles on them. "Amber McKenzie" Sent by: histonet-bounces@lists.utsouthwestern.edu 08/04/2008 11:06 AM To cc Subject [Histonet] stainless steel bucket Does anyone know where I can purchase a stainless steel basket/bucket to wash my cassette lids in? We use xylene and Liqui-Nox to wash them each day and our plastic buckets eventually end up tearing and breaking over time and I have to constantly replace them, so I'm looking for something more stable like stainless steel. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carrolpb <@t> umdnj.edu Mon Aug 4 12:13:26 2008 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Mon Aug 4 12:13:57 2008 Subject: [Histonet] surveillance cameras in the lab In-Reply-To: <0K530075L5AGUNI0@umduwc01.umdnj.edu> References: <0K530075L5AGUNI0@umduwc01.umdnj.edu> Message-ID: <489738B6.2020103@umdnj.edu> > In these times of terror concerns I am not sure I would work in a place where I did not feel secure and the use of these devices > help in that matter. Wasn't it Ben Franklin who said "He who sacrifices freedom for security deserves neither"? From lhadley <@t> iupui.edu Mon Aug 4 12:14:08 2008 From: lhadley <@t> iupui.edu (Baldridge, Lee Ann) Date: Mon Aug 4 12:14:20 2008 Subject: [Histonet] surveillance cameras in the lab In-Reply-To: <200808041648.m74Gm1OH000474@kinison.uits.indiana.edu> References: <000e01c8f633$16b9cee0$3d02a8c0@plab.local> <200808041648.m74Gm1OH000474@kinison.uits.indiana.edu> Message-ID: <5E6A94F8037F4F49B738F5B6AD16952210D8F33F95@iu-mssg-mbx09.ads.iu.edu> This is my take on the situation: "They who can give up essential liberty to obtain a little temporary safety deserve neither liberty nor safety." -Ben Franklin It doesn't matter how many cameras there are, if someone wants to do something they will find a way. I came in this morning to find someone had stolen my computer speakers and one of our printers from our AutoStainers. Lee Ann Baldridge IUSM Indpls., IN. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Monday, August 04, 2008 12:46 PM To: 'Cheri Miller'; Pathrm35@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] surveillance cameras in the lab Come on. In these times of terror concerns I am not sure I would work in a place where I did not feel secure and the use of these devices help in that matter. We built a brand new University of Colorado Health Sciences Center and there are cameras all over the place as well as lock down. If you do not have an access card you cannot get into the labs. This was a pain at first but with all the crazy's we have to worry about out there it now makes me feel better. I just read in the paper this morning about a researcher whose house was bombed by Peta types for doing animal research, and we have had all sorts of disturbances over the years with precious research animals being released, protests, etc. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Monday, August 04, 2008 7:08 AM To: Pathrm35@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] surveillance cameras in the lab That is B.S....I wouldn't work in a lab that used covalence camera's for what ever reason Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathrm35@comcast.net Sent: Monday, August 04, 2008 5:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] surveillance cameras in the lab I was wondering how many techs out there have cameras in their labs, either for security or to monitor employees. I went to work Sunday night and noticed that 4 cameras were installed in the lab over the weekend, with more to come. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rosenfeldtek <@t> hotmail.com Mon Aug 4 12:14:43 2008 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Mon Aug 4 12:14:47 2008 Subject: [Histonet] RE surveillance cameras and terrorism--baloney In-Reply-To: References: <000e01c8f633$16b9cee0$3d02a8c0@plab.local> Message-ID: I work in a secure building--you need proximty cards to get in, use the elevators, etc. But cameras in the lab seem rather much. If you have a Union, I woud consider make a greivance out of it--unsafe and hostile work environment, kind of thing. Jerry Ricks Research Scientist University of Washington Department of Pathology.> From: pruegg@ihctech.net> To: cmiller@physlab.com; Pathrm35@comcast.net; histonet@lists.utsouthwestern.edu> Date: Mon, 4 Aug 2008 10:46:26 -0600> Subject: RE: [Histonet] surveillance cameras in the lab> CC: > > Come on.> In these times of terror concerns I am not sure I would work in a place> where I did not feel secure and the use of these devices help in that> matter.> We built a brand new University of Colorado Health Sciences Center and there> are cameras all over the place as well as lock down. If you do not have an> access card you cannot get into the labs. This was a pain at first but with> all the crazy's we have to worry about out there it now makes me feel> better. > I just read in the paper this morning about a researcher whose house was> bombed by Peta types for doing animal research, and we have had all sorts of> disturbances over the years with precious research animals being released,> protests, etc.> Patsy> > Patsy Ruegg, HT(ASCP)QIHC> IHCtech> 12635 Montview Blvd. #215> Aurora, CO 80045> 720-859-4060> fax 720-859-4110> pruegg@ihctech.net> www.ihctech.net> www.ihcrg.org > > > > -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller> Sent: Monday, August 04, 2008 7:08 AM> To: Pathrm35@comcast.net; histonet@lists.utsouthwestern.edu> Subject: RE: [Histonet] surveillance cameras in the lab> > That is B.S....I wouldn't work in a lab that used covalence camera's for> what ever reason> > Cheryl Miller HT (ASCP)> Histology Supervisor> Physicians Laboratory,P.C.> Omaha, Ne. > 402 738 5052> > -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of> Pathrm35@comcast.net> Sent: Monday, August 04, 2008 5:21 AM> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] surveillance cameras in the lab> > I was wondering how many techs out there have cameras in their labs, either> for security or to monitor employees. I went to work Sunday night and> noticed that 4 cameras were installed in the lab over the weekend, with more> to come.> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If> you are not the addressee intended / indicated or agent responsible for> delivering it to the addressee, you are hereby notified that you are in> possession of confidential and privileged information. Any dissemination,> distribution, or copying of this e-mail is strictly prohibited. If you have> received this message in error, please notify the sender immediately and> delete this email from your system.> > > > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If> you are not the addressee intended / indicated or agent responsible for> delivering it to the addressee, you are hereby notified that you are in> possession of confidential and privileged information. Any dissemination,> distribution, or copying of this e-mail is strictly prohibited. If you have> received this message in error, please notify the sender immediately and> delete this email from your system.> > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Get more from your digital life. Find out how. http://www.windowslive.com/default.html?ocid=TXT_TAGLM_WL_Home2_082008 From bill501 <@t> mindspring.com Mon Aug 4 12:18:56 2008 From: bill501 <@t> mindspring.com (Bill) Date: Mon Aug 4 12:19:12 2008 Subject: [Histonet] surveillance cameras in the lab In-Reply-To: <200808041246.1kq3cU5gg3Nl3oJ1@wanamaker.mail.atl.earthlink.net> References: <200808041246.1kq3cU5gg3Nl3oJ1@wanamaker.mail.atl.earthlink.net> Message-ID: At 10:46 AM -0600 8/4/08, Patsy Ruegg wrote: >In these times of terror concerns I am not sure I would work in a place >where I did not feel secure and the use of these devices help in that >matter. "He who sacrifices freedom for security deserves neither." -Benjamin Franklin BB From sharon.osborn <@t> comcast.net Mon Aug 4 12:20:11 2008 From: sharon.osborn <@t> comcast.net (sharon.osborn@comcast.net) Date: Mon Aug 4 12:20:17 2008 Subject: [Histonet] stainless steel container Message-ID: <080420081720.9819.48973A4B0002BC1F0000265B2215586394029D010D9C01D202019D0E089C@comcast.net> Amber, consider looking in a kitchen ware department for stainless steel mixing bowls or tall cannister sets with the appropriate sized containers that you want. Also, commercial kitchen/restuarant suppliers will have large stainless steel containers as well as varying sizes. If you have a Smart and Final, they sometimes have cooking utensils. sharon osborn, BS,HT(ASCP)CT LabVision ThermoFisher Scientific Fremont, CA Message: 11 Date: Mon, 4 Aug 2008 11:06:26 -0500 From: "Amber McKenzie" Subject: [Histonet] stainless steel bucket To: Message-ID: <03C921A1EAF7F541B16543F6EC6A4B3701D3B09F@giamail2.Gia.com> Content-Type: text/plain; charset="US-ASCII" Does anyone know where I can purchase a stainless steel basket/bucket to wash my cassette lids in? We use xylene and Liqui-Nox to wash them each day and our plastic buckets eventually end up tearing and breaking over time and I have to constantly replace them, so I'm looking for something more stable like stainless steel. From Susan.Weber2 <@t> va.gov Mon Aug 4 12:25:20 2008 From: Susan.Weber2 <@t> va.gov (Weber, Susan (VHACLE)) Date: Mon Aug 4 12:25:27 2008 Subject: [Histonet] surveillance cameras in the lab In-Reply-To: References: <000e01c8f633$16b9cee0$3d02a8c0@plab.local> Message-ID: <16C83872A53F4346AA9C3A18E3A3AAB903F76E16@VHAV10MSGA1.v10.med.va.gov> Yes. Security is of the highest priority for these matters, however, were the cameras in the "public" places? Entrances and exits which need to be and usually are monitored I would understand. We also have lockdown procedures and cameras in the "public" areas and key card accesses which, yes are a pain, but necessary, especially in these times. It is also quite clear that you are under video surveillance (there are signs POSTED everywhere!) Four cameras, which seem to be "monitoring" the workforce I do have a problem with. Silently installing them on a weekend without benefit of explanation, I have a problem with. Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Monday, August 04, 2008 12:46 PM To: 'Cheri Miller'; Pathrm35@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] surveillance cameras in the lab Come on. In these times of terror concerns I am not sure I would work in a place where I did not feel secure and the use of these devices help in that matter. We built a brand new University of Colorado Health Sciences Center and there are cameras all over the place as well as lock down. If you do not have an access card you cannot get into the labs. This was a pain at first but with all the crazy's we have to worry about out there it now makes me feel better. I just read in the paper this morning about a researcher whose house was bombed by Peta types for doing animal research, and we have had all sorts of disturbances over the years with precious research animals being released, protests, etc. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Monday, August 04, 2008 7:08 AM To: Pathrm35@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] surveillance cameras in the lab That is B.S....I wouldn't work in a lab that used covalence camera's for what ever reason Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathrm35@comcast.net Sent: Monday, August 04, 2008 5:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] surveillance cameras in the lab I was wondering how many techs out there have cameras in their labs, either for security or to monitor employees. I went to work Sunday night and noticed that 4 cameras were installed in the lab over the weekend, with more to come. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lchung <@t> ppmh.org Mon Aug 4 12:27:31 2008 From: lchung <@t> ppmh.org (Chung, Luong) Date: Mon Aug 4 12:27:58 2008 Subject: [Histonet] surveillance cameras in the lab In-Reply-To: <5E6A94F8037F4F49B738F5B6AD16952210D8F33F95@iu-mssg-mbx09.ads.iu.edu> Message-ID: <86691924ECCDBE4F82CCAB553424607101DC696C@exchange2.phoebe.com> You should call up one of the Television networks and make a "reality show" out of it. Just a thought. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Baldridge, Lee Ann Sent: Monday, August 04, 2008 1:14 PM To: Patsy Ruegg; 'Cheri Miller'; Pathrm35@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] surveillance cameras in the lab This is my take on the situation: "They who can give up essential liberty to obtain a little temporary safety deserve neither liberty nor safety." -Ben Franklin It doesn't matter how many cameras there are, if someone wants to do something they will find a way. I came in this morning to find someone had stolen my computer speakers and one of our printers from our AutoStainers. Lee Ann Baldridge IUSM Indpls., IN. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Monday, August 04, 2008 12:46 PM To: 'Cheri Miller'; Pathrm35@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] surveillance cameras in the lab Come on. In these times of terror concerns I am not sure I would work in a place where I did not feel secure and the use of these devices help in that matter. We built a brand new University of Colorado Health Sciences Center and there are cameras all over the place as well as lock down. If you do not have an access card you cannot get into the labs. This was a pain at first but with all the crazy's we have to worry about out there it now makes me feel better. I just read in the paper this morning about a researcher whose house was bombed by Peta types for doing animal research, and we have had all sorts of disturbances over the years with precious research animals being released, protests, etc. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Monday, August 04, 2008 7:08 AM To: Pathrm35@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] surveillance cameras in the lab That is B.S....I wouldn't work in a lab that used covalence camera's for what ever reason Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathrm35@comcast.net Sent: Monday, August 04, 2008 5:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] surveillance cameras in the lab I was wondering how many techs out there have cameras in their labs, either for security or to monitor employees. I went to work Sunday night and noticed that 4 cameras were installed in the lab over the weekend, with more to come. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Disclaimer: The HIPAA Final Privacy Rule requires covered entities to safeguard certain Protected Health Information (PHI) related to a person's healthcare. Information being faxed to you may include PHI after appropriate authorization from the patient or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain PHI in a safe and secure manner. You may not re-disclose without additional patient consent or as required by law. Unauthorized re-disclosure or failure to safeguard PHI could subject you to penalties described in federal (HIPAA) and state law. If you the reader of this message are not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, please notify us immediately and destroy the related message. From jqb7 <@t> cdc.gov Mon Aug 4 12:27:47 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Mon Aug 4 12:28:16 2008 Subject: [Histonet] surveillance cameras in the lab In-Reply-To: References: <200808041246.1kq3cU5gg3Nl3oJ1@wanamaker.mail.atl.earthlink.net> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A7F23E2A@LTA3VS011.ees.hhs.gov> I don't think Ben Franklin's quote applies here: we are talking about a place of employment not your home. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bill Sent: Monday, August 04, 2008 1:19 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] surveillance cameras in the lab At 10:46 AM -0600 8/4/08, Patsy Ruegg wrote: >In these times of terror concerns I am not sure I would work in a place >where I did not feel secure and the use of these devices help in that >matter. "He who sacrifices freedom for security deserves neither." -Benjamin Franklin BB _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carrolpb <@t> umdnj.edu Mon Aug 4 12:33:11 2008 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Mon Aug 4 12:33:45 2008 Subject: [Histonet] surveillance cameras in the lab In-Reply-To: <1CE1847DFEA0A647B1CCDE4108EA60A7F23E2A@LTA3VS011.ees.hhs.gov> References: <200808041246.1kq3cU5gg3Nl3oJ1@wanamaker.mail.atl.earthlink.net> <1CE1847DFEA0A647B1CCDE4108EA60A7F23E2A@LTA3VS011.ees.hhs.gov> Message-ID: <48973D57.7070901@umdnj.edu> > I don't think Ben Franklin's quote applies here: we are talking about a place of employment not your home. though we're quickly approaching content off-topic for this list, id like to raise the rhetorical question: civil-liberties dont apply in the workplace? i dont know about y'all, but i spend as much time at work as i do at home, by and large, and would be rather bothered if cameras appeared with no justification/explanation. just as i have nothing to hide from said cameras, they have no reason to watch me, by default. From lhadley <@t> iupui.edu Mon Aug 4 12:36:24 2008 From: lhadley <@t> iupui.edu (Baldridge, Lee Ann) Date: Mon Aug 4 12:36:31 2008 Subject: [Histonet] surveillance cameras in the lab In-Reply-To: <1CE1847DFEA0A647B1CCDE4108EA60A7F23E2A@LTA3VS011.ees.hhs.gov> References: <200808041246.1kq3cU5gg3Nl3oJ1@wanamaker.mail.atl.earthlink.net> <1CE1847DFEA0A647B1CCDE4108EA60A7F23E2A@LTA3VS011.ees.hhs.gov> Message-ID: <5E6A94F8037F4F49B738F5B6AD16952210D8F33F99@iu-mssg-mbx09.ads.iu.edu> Oh but it does. Our liberties are eroded away a little bit at a time. Where does it stop? Lee Ann Baldridge IUSM Indpls.,IN. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Monday, August 04, 2008 1:28 PM To: Bill; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] surveillance cameras in the lab I don't think Ben Franklin's quote applies here: we are talking about a place of employment not your home. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bill Sent: Monday, August 04, 2008 1:19 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] surveillance cameras in the lab At 10:46 AM -0600 8/4/08, Patsy Ruegg wrote: >In these times of terror concerns I am not sure I would work in a place >where I did not feel secure and the use of these devices help in that >matter. "He who sacrifices freedom for security deserves neither." -Benjamin Franklin BB _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Mon Aug 4 12:39:40 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Mon Aug 4 12:39:56 2008 Subject: [Histonet] surveillance cameras in the lab In-Reply-To: <48973D57.7070901@umdnj.edu> References: <200808041246.1kq3cU5gg3Nl3oJ1@wanamaker.mail.atl.earthlink.net> <1CE1847DFEA0A647B1CCDE4108EA60A7F23E2A@LTA3VS011.ees.hhs.gov> <48973D57.7070901@umdnj.edu> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A7F23E2E@LTA3VS011.ees.hhs.gov> I agree they should have been notified and all that but my point is that if I do not like the policies where I work I am free to go somewhere else. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Peter Carroll Sent: Monday, August 04, 2008 1:33 PM Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] surveillance cameras in the lab > I don't think Ben Franklin's quote applies here: we are talking about a place of employment not your home. though we're quickly approaching content off-topic for this list, id like to raise the rhetorical question: civil-liberties dont apply in the workplace? i dont know about y'all, but i spend as much time at work as i do at home, by and large, and would be rather bothered if cameras appeared with no justification/explanation. just as i have nothing to hide from said cameras, they have no reason to watch me, by default. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carrolpb <@t> umdnj.edu Mon Aug 4 12:42:05 2008 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Mon Aug 4 12:42:55 2008 Subject: [Histonet] surveillance cameras in the lab In-Reply-To: <1CE1847DFEA0A647B1CCDE4108EA60A7F23E2E@LTA3VS011.ees.hhs.gov> References: <200808041246.1kq3cU5gg3Nl3oJ1@wanamaker.mail.atl.earthlink.net> <1CE1847DFEA0A647B1CCDE4108EA60A7F23E2A@LTA3VS011.ees.hhs.gov> <48973D57.7070901@umdnj.edu> <1CE1847DFEA0A647B1CCDE4108EA60A7F23E2E@LTA3VS011.ees.hhs.gov> Message-ID: <48973F6D.1030503@umdnj.edu> > if I do not like the policies where I work I am free to go somewhere else. thats a useful option... until the point where every viable place you could work is subject to the same draconian policy, cameras and lack of explanation. From froyer <@t> bitstream.net Mon Aug 4 12:45:23 2008 From: froyer <@t> bitstream.net (Ford Royer) Date: Mon Aug 4 12:45:46 2008 Subject: [Histonet] stainless steel bucket In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B3701D3B09F@giamail2.Gia.com> References: <03C921A1EAF7F541B16543F6EC6A4B3701D3B09F@giamail2.Gia.com> Message-ID: <008101c8f659$df5aa100$7701a80a@Ford> Not sure if this is what you?re looking for, but we carry stainless steel buckets/beakers/storage containers. Link: http://stores.implex.net/minnesotamedical/index.php?main_page=index&cPath=56 _193&zenid=b780c44693573ef63f1b26b8b8bcb664 Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL:? 612-839-1046 Phone:? 763-542-8725 Fax:? 763-546-4830 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Monday, August 04, 2008 11:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] stainless steel bucket Does anyone know where I can purchase a stainless steel basket/bucket to wash my cassette lids in? We use xylene and Liqui-Nox to wash them each day and our plastic buckets eventually end up tearing and breaking over time and I have to constantly replace them, so I'm looking for something more stable like stainless steel. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From micro <@t> superlink.net Mon Aug 4 12:55:23 2008 From: micro <@t> superlink.net (Markus F. Meyenhofer) Date: Mon Aug 4 12:55:31 2008 Subject: [Histonet] surveillance cameras in the lab References: <0K530075L5AGUNI0@umduwc01.umdnj.edu> <489738B6.2020103@umdnj.edu> Message-ID: <059201c8f65b$45dce360$0f893cd1@DJ4VDH31> Well said (Ben's words) Thank you! ----- Original Message ----- From: "Peter Carroll" Cc: Sent: Monday, August 04, 2008 1:13 PM Subject: Re: [Histonet] surveillance cameras in the lab > > In these times of terror concerns I am not sure I would work in a > place where I did not feel secure and the use of these devices > > help in that matter. > > Wasn't it Ben Franklin who said "He who sacrifices freedom for security > deserves neither"? > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From liz <@t> premierlab.com Mon Aug 4 12:59:26 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Mon Aug 4 12:59:31 2008 Subject: [Histonet] Cross polarization and collagen I and III Message-ID: Hello again If anyone remembers my post late last week on cross polarization the reason why I asked that is that I have a paper of a technique that used cross polarization and picrosirius stained slides to differenciate collagen I fibers from collagen III fibers. In the paper they basically stated the the thicker collagen I fibers looked orange-red and the thinner collagen III fibers looked green. I have not heard of this before and was a bit confused, I thought that picrosirus just stained for collagen, so I initally thought that maybe cross polarization is different than routine polarization. I have since found out that they are one in the same. My question now is there anyone out there familar with using picrosirus to differenciate collagen III from collagen I and can you do that? Thanks in advance PS - I do have the paper if anyone is intersted in looking at it. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 From jwatson <@t> gnf.org Mon Aug 4 13:27:20 2008 From: jwatson <@t> gnf.org (James Watson) Date: Mon Aug 4 13:27:26 2008 Subject: [Histonet] Cross polarization and collagen I and III In-Reply-To: References: Message-ID: Liz, I have watching this thread to see if someone mentions a rotating stage. Having serviced microscopes in the past, I know that there are polarizing microscopes that are set up with a rotating polarizer below the condenser and a stationary analyzer (same as a Polarizer but called an analyzer because it is above the specimen) above the objectives. Then once you have aligned the polarizer and analyzer to get the polarized light you can rotate the stage of the microscope to see the different colors depending on the alignment of the crystal or fiber. Picrosirus red will stain other tissue elements and the conformation of what is stained is collagen is with polarization (Histological & Histochemical Methods J.A. Kiernan pg. 150). This is a good reference on this technique. Please send me a copy of this paper. Thank you James Watson HT ASCP Facilities Manager of Histology GNF Genomics Institute of the Novartis Research Foundation Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Monday, August 04, 2008 10:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cross polarization and collagen I and III Hello again If anyone remembers my post late last week on cross polarization the reason why I asked that is that I have a paper of a technique that used cross polarization and picrosirius stained slides to differenciate collagen I fibers from collagen III fibers. In the paper they basically stated the the thicker collagen I fibers looked orange-red and the thinner collagen III fibers looked green. I have not heard of this before and was a bit confused, I thought that picrosirus just stained for collagen, so I initally thought that maybe cross polarization is different than routine polarization. I have since found out that they are one in the same. My question now is there anyone out there familar with using picrosirus to differenciate collagen III from collagen I and can you do that? Thanks in advance PS - I do have the paper if anyone is intersted in looking at it. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ASelf <@t> georgetownhospitalsystem.org Mon Aug 4 13:39:45 2008 From: ASelf <@t> georgetownhospitalsystem.org (Amy Self) Date: Mon Aug 4 13:39:49 2008 Subject: [Histonet] Workload Recording Message-ID: Histonetters, Does anyone have any info and/or charts on workload recording in histology that they would be willing to share with me? Thanks in advance, Amy Georgetown Hospital System Dept. of Pathology NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From gmartin <@t> marshallmedical.org Mon Aug 4 13:43:57 2008 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Mon Aug 4 13:44:14 2008 Subject: [Histonet] Workload Recording In-Reply-To: References: Message-ID: <6ED9D4252F278841A0593D3D788AF24C02FC5E54@mailsvr.MARSHMED.local> Forget logs ... just get some cameras :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Monday, August 04, 2008 11:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Workload Recording Histonetters, Does anyone have any info and/or charts on workload recording in histology that they would be willing to share with me? Thanks in advance, Amy Georgetown Hospital System Dept. of Pathology NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mbisher <@t> Princeton.EDU Mon Aug 4 13:44:49 2008 From: mbisher <@t> Princeton.EDU (Peggy Bisher) Date: Mon Aug 4 13:44:58 2008 Subject: [Histonet] HT schools In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B3701D3B0C3@giamail2.Gia.com> Message-ID: I would say that teaching Electron Microscopy is the same. There are a few schools (two, I believe) that offer an official degree (it's an Associate Degree) in Electron Microscopy, but most of my learning has been on the job and that is how I work with my students - one on one. Here at Princeton we offer a class in electron optics, but it is just theory. My first job was at the National Institutes of Health and my boss there had a degree in theoretical physics. He gave me a few books and I taught myself, at least in the beginning. He knew the instrument, but nothing about sample prep. The Microscopy Society of America does offer a Electron Microscopist Certification for those who want to have this. But I have been doing EM since 1980, so never felt the need to be certified. Margaret E. Bisher Electron Microscopy & Histology Core Facility Manager Department of Molecular Biology Princeton University Moffett Laboratory, Room 113 Princeton, New Jersey Office: (609) 258-7026 Fax: (609) 258-8468 mbisher@princeton.edu On 8/4/08 1:08 PM, "Amber McKenzie" wrote: > Even if the online schools teach the theory of Histology, they expect the > supervisor/techs to teach the potential HT's how to perform Histology. Even > though there is no practical anymore, potential HT's have to be taught the > hands on part of the job with OJT. Even though I've heard many people say > that there is no more OJT, really there is b/c if these future HT's don't > attend an actual HT school where there are classrooms/practice labs full of > teachers, then we as working HT's have to teach our future co-workers how to > do our job so that we'll have more people to pick from to hire. Does any > other profession handle their future employee's like this or is Histology in a > category of its own? > > > > -----Original Message----- > From: Patsy Ruegg [mailto:pruegg@ihctech.net] > Sent: Monday, August 04, 2008 11:46 AM > To: 'Cheri Miller'; rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; > Amber McKenzie > Subject: RE: [Histonet] HT schools > > > > Remember that now there is no practical portion of the HT exam, so they are > > not being tested on hands on experiences anyway. The most difficult problem > > I have with training people on the job (and I have trained many) is that now > > they are not prepared to take the exam because they are examined all on > > theory. I have some really well trained people who can do the work really > > well, but they have a hard time taking the computer test which they pretty > > much have to memorize out of books. > > Patsy > > > > Patsy Ruegg, HT(ASCP)QIHC > > IHCtech > > 12635 Montview Blvd. #215 > > Aurora, CO 80045 > > 720-859-4060 > > fax 720-859-4110 > > pruegg@ihctech.net > > www.ihctech.net > > www.ihcrg.org > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller > > Sent: Monday, August 04, 2008 7:05 AM > > To: rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; 'Amber McKenzie' > > Subject: RE: [Histonet] HT schools > > > > Someone has to teach them the "hands on" part of histology. I do not leave > > this to my staff. I teach / give them the skills they need to perform the > > practical part of their profession. As their supervisor I am very much > > involved in their training. I am sure I am not the only one.?? > > > > Cheryl Miller HT (ASCP) > > Histology Supervisor > > Physicians Laboratory,P.C. > > Omaha, Ne. > > 402 738 5052 > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > > Sent: Friday, August 01, 2008 2:42 PM > > To: histonet@lists.utsouthwestern.edu; Amber McKenzie > > Subject: Re: [Histonet] HT schools > > > > Wrong! The advantage of the "on line" or "distance learning" courses is that > > they provide the theory on line while you are working at a given laboratory > > doing your training (or even as part of your daily work) so there is no > > "actual training" to be done by the supervisor. > > At this moment NAACLS has 30 HT and 3 HTL accredited programs only, with an > > overall capacity of about 300-325 students, and this will not be enough to > > take care of all the retiring histotechs. > > Costs is one of the reasons why the number of HTs schools is dwindling. > > Ren? J. > > > > > > --- On Fri, 8/1/08, Amber McKenzie wrote: > > > > From: Amber McKenzie > > Subject: [Histonet] HT schools > > To: histonet@lists.utsouthwestern.edu > > Date: Friday, August 1, 2008, 1:23 PM > > > > Where are all the HT accredited schools and why aren't there more out > > there? I've seen the online classes' people can take, but that > > requires > > them to be trained in a lab, as well, for the "hands on" part. So, > > actually the supervisor still has to train potential HT's "on the > > job" > > before they can sit for the board exam. Right? > > > > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Mon Aug 4 13:50:48 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Aug 4 13:51:01 2008 Subject: [Histonet] p16 on Ventana In-Reply-To: <44780C571F28624DBB446DE55C4D733A021E0A51@EXCHANGEBE1.carle.com> Message-ID: <000001c8f663$05e9c220$d00f7ca5@lurie.northwestern.edu> Both Labvision and novacastra (Leica vision biosystems) sell it. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Monday, August 04, 2008 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 on Ventana Hey Histonetters! Is anyone out there in histoworld running p16 on the Ventana system? If so, can you provide us with your protocol and where you are ordering it from? We are aware that MTM is presently the only vendor for p16 but it is rather pricey and are wondering if there are any alternatives available out there. Once again thank you so much for your help. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tjasper <@t> copc.net Mon Aug 4 13:57:43 2008 From: tjasper <@t> copc.net (Thomas Jasper) Date: Mon Aug 4 13:57:49 2008 Subject: [Histonet] surveillance cameras in the lab References: <080420081021.7972.4896D816000E927500001F242212020784CACC039D089B0EAF@comcast.net> Message-ID: <90354A475B420441B2A0396E5008D4965E2133@copc-sbs.COPC.local> Well said Barry, and I have to agree with the comments posted by Joe Nocito and Glen Dawson. I think this is paranoia (will destroy ya) driven behavior. Someone commented that they were working in a high crime area at one time (and then left). I took this to be a security justification for having these cameras. Cameras aimed at technicians working doesn't address that type of security in my book. These days, lab access, generally is by electronic key code and hospitals/medical centers/research facilities have fairly extensive security personnel and policies in place. Also anyone that PETA has tried to harm away from their lab is not made safer by cameras in the work place. That's a matter for local law enforcement. Don't get me wrong, I'm interested in having a safe and secure work environment. Having four cameras trained on my staff or myself doesn't make me feel safe and secure. Someone mentioned employees napping, well, why is that happening? Supervisors, managers and administrators that are worth their salt ought to be aware of these types of problems without having a camera "tattle" for them. I'm sorry, but a camera is not a substitute for effective supervision. I'm not excusing an employee here. I'm just saying this is not the answer. I do feel this is an infringement on people's privacy to an extent. Like Barry said, there's a trust issue here. You don't trust people? Maybe they shouldn't be working for you...and maybe you should never eat in a restaurant again either. I hate to say this but...I think some people get a charge out of spying on others. Why do I feel like some of those TSA people at airports enjoy rummaging through luggage? Same thing...I realize we need airport security and I don't object to it when travelling. But cameras in the work place, the line should be drawn somewhere. The money and time could be much better spent as well. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, OR 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Monday, August 04, 2008 4:05 AM To: Pathrm35@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] surveillance cameras in the lab In my experience this type of situation occurs when a situation has arisen and the administration does not know how to take care of it directly. If there is such a problem then the administration needs to discuss this at least with the supervisors and preferably with all the employees. In a lab, there must be two way trust for a team to work. Seems to me that this trust has just been lost. The question is also of what happens to any recordings. I can see many abuses of such a system. Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Pathrm35@comcast.net Sent: Mon 8/4/2008 5:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] surveillance cameras in the lab I was wondering how many techs out there have cameras in their labs, either for security or to monitor employees. I went to work Sunday night and noticed that 4 cameras were installed in the lab over the weekend, with more to come. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vgrover <@t> polysciences.com Mon Aug 4 13:56:07 2008 From: vgrover <@t> polysciences.com (Valantou Grover) Date: Mon Aug 4 13:58:14 2008 Subject: [Histonet] RE: Histonet Digest, Vol 57, Issue 5, re:p16 on ventana Message-ID: <000001c8f663$c0f9a300$5200a8c0@USWARD13ZFB71> Hello All, We used the following protocol at PH for p16 you can use the check marks in the protocol to signify selection: Paraffin- (selected) Deparaffinization- (selected) Cell Conditioning - (selected) Cell Conditioner #1- (selected) Mild CC1- (selected) Antibody- (selected) Apply 1 drop of [prep kit # designation for p16](Antibody) and incubate for {0 hr 32 min) The rest is up to you to include counter stain or not(usually hematoxylin) Ours was predilute from Cell Marque and we used a prep kit number. Good luck and have a great day. Valantou Grover, HT(ASCP), HTL, PA Biosciences Product Line Manager Polysciences, Inc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 57, Issue 5 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. surveillance cameras in the lab (Paula Pierce) 2. Re: surveillance cameras in the lab (Bill) 3. surveillance cameras in the lab (Paula Pierce) 4. RE: surveillance cameras in the lab (Bartlett, Jeanine (CDC/CCID/NCZVED)) 5. RE: surveillance cameras in the lab (Weber, Susan (VHACLE)) 6. RE: surveillance cameras in the lab (Martin, Gary) 7. RE: tissue falling out of paraffin block (Monfils, Paul) 8. PTAH staining on Carnoy's fixed tissue (Trajkovic, Dusko) 9. p16 on Ventana (Sharon.Davis-Devine) 10. surveillance cameras in the lab (Troutman, Kenneth A) 11. stainless steel bucket (Amber McKenzie) 12. RE: p16 on Ventana (Patsy Ruegg) 13. Tissue Microarray Location Markers (Thom Jensen) 14. RE: surveillance cameras in the lab (Patsy Ruegg) 15. RE: tissue falling out of paraffin block (Patsy Ruegg) 16. RE: stainless steel bucket (Bonner, Janet) 17. Re: Rabbit IgG control (R C) 18. RE: HT schools (Patsy Ruegg) 19. RE: surveillance cameras in the lab (Patsy Ruegg) 20. QIHC Certification (William Connor) ---------------------------------------------------------------------- Message: 1 Date: Mon, 4 Aug 2008 07:02:12 -0700 (PDT) From: Paula Pierce Subject: [Histonet] surveillance cameras in the lab To: Histonet Message-ID: <419055.10912.qm@web50106.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I thought about blacking out the lenses too. The worst part of this whole situation is that the cameras suddenly appeared. The tech just happened to notice them. It is totally unacceptable to me that an institution would not inform the employees they were going to install cameras. This may be CYA for the powers that be. RUN! Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 631 N. Broadway Ave. Moore, OK 73160 405-570-6679 cell 405-759-3953 lab contact@excaliburpathology.com www.excaliburpathology.com ------------------------------ Message: 2 Date: Mon, 4 Aug 2008 09:02:27 -0500 From: Bill Subject: Re: [Histonet] surveillance cameras in the lab To: Pathrm35@comcast.net, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="us-ascii" At 10:21 AM +0000 8/4/08, Pathrm35@comcast.net wrote: >>>I was wondering how many techs out there have cameras in their labs, either for security or to monitor employees. I went to work Sunday night and noticed that 4 cameras were installed in the lab over the weekend, with more to come.<<< I would use immersion oil on the camera lenses. -- ______________ Bill Blank, MD Heartland Lab ------------------------------ Message: 3 Date: Mon, 4 Aug 2008 07:03:53 -0700 (PDT) From: Paula Pierce Subject: [Histonet] surveillance cameras in the lab To: Rosa Fields , Histonet Message-ID: <499718.21489.qm@web50109.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hydrofluoric acid etches glass ;) Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 631 N. Broadway Ave. Moore, OK 73160 405-570-6679 cell 405-759-3953 lab contact@excaliburpathology.com www.excaliburpathology.com ----- Original Message ---- From: Rosa Fields To: Mike Pence ; Pathrm35@comcast.net; histonet@lists.utsouthwestern.edu Sent: Monday, August 4, 2008 8:46:02 AM Subject: RE: [Histonet] surveillance cameras in the lab You could put a nice coat of paraffin on the lenses! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Monday, August 04, 2008 8:31 AM To: Pathrm35@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] surveillance cameras in the lab One way of finding out who is "watching you" is to blackout the lenses. This should get a quick response! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathrm35@comcast.net Sent: Monday, August 04, 2008 5:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] surveillance cameras in the lab I was wondering how many techs out there have cameras in their labs, either for security or to monitor employees. I went to work Sunday night and noticed that 4 cameras were installed in the lab over the weekend, with more to come. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Mon, 4 Aug 2008 10:17:38 -0400 From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" Subject: RE: [Histonet] surveillance cameras in the lab To: "Paula Pierce" , Histonet Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A7F23E22@LTA3VS011.ees.hhs.gov> Content-Type: text/plain; charset=iso-8859-1 I am not against the idea of the cameras...but I would definitely have an issue with no one being notified that they were going to be installed and why. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Monday, August 04, 2008 10:02 AM To: Histonet Subject: [Histonet] surveillance cameras in the lab I thought about blacking out the lenses too. The worst part of this whole situation is that the cameras suddenly appeared. The tech just happened to notice them. It is totally unacceptable to me that an institution would not inform the employees they were going to install cameras. This may be CYA for the powers that be. RUN! Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 631 N. Broadway Ave. Moore, OK 73160 405-570-6679 cell 405-759-3953 lab contact@excaliburpathology.com www.excaliburpathology.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Mon, 4 Aug 2008 10:34:05 -0400 From: "Weber, Susan (VHACLE)" Subject: RE: [Histonet] surveillance cameras in the lab To: "Bartlett, Jeanine (CDC/CCID/NCZVED)" , "Paula Pierce" , "Histonet" Message-ID: <16C83872A53F4346AA9C3A18E3A3AAB903F76E15@VHAV10MSGA1.v10.med.va.gov> Content-Type: text/plain; charset="iso-8859-1" Was this through out the whole lab, or just Histology. The other departments may be interested in knowing they are being "observed" dare I say "watched", "spied upon", etc. It is highly unacceptable to me that there is no notification to the "workers" that big bro is hovering overhead. Also unacceptable is the fact that "they" are not giving a very good "reason" for the install. BTW- wear proper PPE so they can't tell who is "cleaning" that lens with the nice immersion oil. Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Monday, August 04, 2008 10:18 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] surveillance cameras in the lab I am not against the idea of the cameras...but I would definitely have an issue with no one being notified that they were going to be installed and why. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Monday, August 04, 2008 10:02 AM To: Histonet Subject: [Histonet] surveillance cameras in the lab I thought about blacking out the lenses too. The worst part of this whole situation is that the cameras suddenly appeared. The tech just happened to notice them. It is totally unacceptable to me that an institution would not inform the employees they were going to install cameras. This may be CYA for the powers that be. RUN! Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 631 N. Broadway Ave. Moore, OK 73160 405-570-6679 cell 405-759-3953 lab contact@excaliburpathology.com www.excaliburpathology.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Mon, 4 Aug 2008 07:42:47 -0700 From: "Martin, Gary" Subject: RE: [Histonet] surveillance cameras in the lab To: "Paula Pierce" , "Histonet" Message-ID: <6ED9D4252F278841A0593D3D788AF24C02FC5A1F@mailsvr.MARSHMED.local> Content-Type: text/plain; charset="iso-8859-1" The camera thing is odd! There should be some sort of written policy to accompany this process. I was a school board member for several years and the other members insisted on installing cameras in the buss'. This, as one responder mentioned, caused all kinds of unanticipated problems. The first and foremost was that all information gathered was after the fact and the images were not clear enough to assign blame. Also it was surprising who got their hand on those recordings for their viewing pleasure. Needless to say the very expensive cameras were removed after two lawsuits by parents. I do agree with one of the responders ... that this is something the administration is not handling directly. They at least owe you an explanation! Gary -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Monday, August 04, 2008 7:02 AM To: Histonet Subject: [Histonet] surveillance cameras in the lab I thought about blacking out the lenses too. The worst part of this whole situation is that the cameras suddenly appeared. The tech just happened to notice them. It is totally unacceptable to me that an institution would not inform the employees they were going to install cameras. This may be CYA for the powers that be. RUN! Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 631 N. Broadway Ave. Moore, OK 73160 405-570-6679 cell 405-759-3953 lab contact@excaliburpathology.com www.excaliburpathology.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Mon, 4 Aug 2008 10:45:40 -0400 From: "Monfils, Paul" Subject: RE: [Histonet] tissue falling out of paraffin block To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835C45@LSRIEXCH1.lsmaster.lifespan.org> Content-Type: text/plain; charset="iso-8859-1" That sounds like incomplete dehydration. A small amount of water remains in the tissue. This prevents full infiltration by both the clearling agent and the paraffin. Once the block is faced off, the water and/or solvent evaporates from the tissue, causing it to shrink and pull away from the paraffin. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Price, Tiffany > Sent: Monday, August 4, 2008 9:56 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] tissue falling out of paraffin block > > Can anyone tell me what would make liver tissue fall out of a paraffin > block after routine processing? We cut a routine H&E slide, and later, > had to cut IHC stains that were ordered. The tissue was fine for the > H&E, but the whole tissue fell out of the block when we tried to re-cut > it, even after re-embedding. > > Thanks for any help > Tiffany > Confidentiality Notice: This email message including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privilege information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy ALL copies of the message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 8 Date: Mon, 4 Aug 2008 07:49:29 -0700 From: "Trajkovic, Dusko" Subject: [Histonet] PTAH staining on Carnoy's fixed tissue To: Message-ID: <3AD0BD3142459B4E9B12CBEAFF2B89B207AC423B@lajamrexm01.amer.pfizer.com> Content-Type: text/plain; charset="us-ascii" Good Morning/Day/Evening Histonet colleagues, Does anyone have a protocol for staining PTAH on Carnoy's fixed tissue? How does the fixative impact the PTAH staining? Any information would be greatly appreciated. Thank you, Dusko Trajkovic ------------------------------ Message: 9 Date: Mon, 4 Aug 2008 09:59:33 -0500 From: "Sharon.Davis-Devine" Subject: [Histonet] p16 on Ventana To: Message-ID: <44780C571F28624DBB446DE55C4D733A021E0A51@EXCHANGEBE1.carle.com> Content-Type: text/plain; charset="us-ascii" Hey Histonetters! Is anyone out there in histoworld running p16 on the Ventana system? If so, can you provide us with your protocol and where you are ordering it from? We are aware that MTM is presently the only vendor for p16 but it is rather pricey and are wondering if there are any alternatives available out there. Once again thank you so much for your help. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com ------------------------------ Message: 10 Date: Mon, 4 Aug 2008 11:03:17 -0500 From: "Troutman, Kenneth A" Subject: [Histonet] surveillance cameras in the lab To: "Histonet" Message-ID: <37DEF9AF72994947AF693956A59B9B660127FF52@mailbe03.mc.vanderbilt.edu> Content-Type: text/plain; charset="iso-8859-1" I used to work in a lab that had surveillance cameras. At the time, we only had one that was over our single grossing station to monitor grossing. (There were some missing tissue accusations that needed to be nipped in the bud, so to say...) I eventually started managing the accessioning department and we installed 4 to monitor specimen receipt to see if anything was accidently thrown in the trash. It was supposed to be a quick reference tool that was designed to keep us from digging in the biohazard trash unnecessarily, however, it proved to be more useful for catching employees napping! (Yes, they were all aware that the cameras existed!) As for cameras in the lab in general, you will probably have to ask upper management (or whoever made the decision to install them) why they are there. Perhaps they may even tell you the answer. Good luck. Ashley Troutman BS, HT(ASCP)QIHC Histopathology Laboratory Department of Pathology Vanderbilt University Medical Center Nashville, TN ------------------------------ Message: 11 Date: Mon, 4 Aug 2008 11:06:26 -0500 From: "Amber McKenzie" Subject: [Histonet] stainless steel bucket To: Message-ID: <03C921A1EAF7F541B16543F6EC6A4B3701D3B09F@giamail2.Gia.com> Content-Type: text/plain; charset="US-ASCII" Does anyone know where I can purchase a stainless steel basket/bucket to wash my cassette lids in? We use xylene and Liqui-Nox to wash them each day and our plastic buckets eventually end up tearing and breaking over time and I have to constantly replace them, so I'm looking for something more stable like stainless steel. ------------------------------ Message: 12 Date: Mon, 4 Aug 2008 10:07:15 -0600 From: "Patsy Ruegg" Subject: RE: [Histonet] p16 on Ventana To: "'Sharon.Davis-Devine'" , Message-ID: Content-Type: text/plain; charset="us-ascii" Sharon, The NSH IHC Resource Group just had a discussion about p16 and some alternatives were offered. NSH members can join the ihcrg online at www.ihcrg.org Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Monday, August 04, 2008 9:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 on Ventana Hey Histonetters! Is anyone out there in histoworld running p16 on the Ventana system? If so, can you provide us with your protocol and where you are ordering it from? We are aware that MTM is presently the only vendor for p16 but it is rather pricey and are wondering if there are any alternatives available out there. Once again thank you so much for your help. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Mon, 4 Aug 2008 16:12:27 +0000 From: Thom Jensen Subject: [Histonet] Tissue Microarray Location Markers To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" I am trying to find out what the best way is to determine the number one spot on a TMA? We use died lung for location markers. One color punch represents a group of punches. What other ways are techs marking their TMA locations? I know there are a variety of ways for documentation of TMAs but I am interested in the TMA block and location marker techniques and materials. Thanks all, Thom HT (ASAP) TMA Technician New TMA instrument go to: arraymold.com _________________________________________________________________ Your PC, mobile phone, and online services work together like never before. http://clk.atdmt.com/MRT/go/108587394/direct/01/ ------------------------------ Message: 14 Date: Mon, 4 Aug 2008 10:15:57 -0600 From: "Patsy Ruegg" Subject: RE: [Histonet] surveillance cameras in the lab To: "'Bill'" , , Message-ID: Content-Type: text/plain; charset="us-ascii" As an employer I am unaware of any regulations requiring you to inform employees that you will install cameras for security or any other reason, you would probably need to post a sign stating that there are cameras being used in the area is all? Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bill Sent: Monday, August 04, 2008 8:02 AM To: Pathrm35@comcast.net; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] surveillance cameras in the lab At 10:21 AM +0000 8/4/08, Pathrm35@comcast.net wrote: >>>I was wondering how many techs out there have cameras in their labs, either for security or to monitor employees. I went to work Sunday night and noticed that 4 cameras were installed in the lab over the weekend, with more to come.<<< I would use immersion oil on the camera lenses. -- ______________ Bill Blank, MD Heartland Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Mon, 4 Aug 2008 10:20:35 -0600 From: "Patsy Ruegg" Subject: RE: [Histonet] tissue falling out of paraffin block To: "'Price, Tiffany'" , Message-ID: Content-Type: text/plain; charset="us-ascii" I see this all the time with animal tissue, I think it is in samples that are particularly bloody after soaking in the ice water bath before cutting, it is like the paraffin does not infiltrate the tissue very well and it is separate from the surrounding paraffin in the block. Usually reembedding does the trick for me. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Price, Tiffany Sent: Monday, August 04, 2008 7:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tissue falling out of paraffin block Can anyone tell me what would make liver tissue fall out of a paraffin block after routine processing? We cut a routine H&E slide, and later, had to cut IHC stains that were ordered. The tissue was fine for the H&E, but the whole tissue fell out of the block when we tried to re-cut it, even after re-embedding. Thanks for any help Tiffany Confidentiality Notice: This email message including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privilege information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy ALL copies of the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Mon, 4 Aug 2008 12:23:03 -0400 From: "Bonner, Janet" Subject: RE: [Histonet] stainless steel bucket To: "Amber McKenzie" , histonet@lists.utsouthwestern.edu Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2748@fhosxchmb006.ADVENTISTCORP.NET> Content-Type: text/plain; charset=iso-8859-1 Ask your surgery Department for a catalog or maybe they have some old pieces they'd be happy to let you have! If you're a Reference Lab with no Surgery, Mopec carries a nice line of Pathology Morgue Equipment. Janet Janet L. Bonner, HTL (ASCP) Pathology Laboratory Florida Hospital Winter Park janet.bonner@FLHOSP.org 407-646-7559 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Amber McKenzie Sent: Mon 8/4/2008 12:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] stainless steel bucket Does anyone know where I can purchase a stainless steel basket/bucket to wash my cassette lids in? We use xylene and Liqui-Nox to wash them each day and our plastic buckets eventually end up tearing and breaking over time and I have to constantly replace them, so I'm looking for something more stable like stainless steel. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= ------------------------------ Message: 17 Date: Mon, 4 Aug 2008 09:25:07 -0700 From: "R C" Subject: [Histonet] Re: Rabbit IgG control To: histonet@lists.utsouthwestern.edu Message-ID: <2a926e3f0808040925s38e922f4mda2156f97b5592d9@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Jackson Immuno Research. On Sat, Aug 2, 2008 at 10:01 AM, wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Rabbit IgG control (Margaryan, Naira) > 2. Alginate beads containing cells (Danielson, Keith) > 3. HT schools (Amber McKenzie) > 4. Christopher Hayden is nowhere near the lab. > (christopher.hayden@novartis.com) > 5. A question for BondMax users (Victoria Baker) > 6. Re: HT schools (Victoria Baker) > 7. RE: A question for BondMax users (Beckham, Sharon) > 8. Bone Marrow Biopsy Needles (Dana Brewer) > 9. Re: HT schools (Rene J Buesa) > 10. processing PFA fixed alginate beads (Gayle Callis) > 11. Methodology for extracting nucleic acids from FFPE Tissue > (Gayle Callis) > 12. RE: Dictation systems (Michael Mihalik) > 13. Re: HT schools (Joe Nocito) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 1 Aug 2008 12:03:09 -0500 > From: "Margaryan, Naira" > Subject: [Histonet] Rabbit IgG control > To: > Message-ID: > < > EE5AF506236CEE4CAFD1A281F5DCFE90021035C8@CMHEXC02EVS.childrensmemorial.org > > > > Content-Type: text/plain; charset="us-ascii" > > Hi Dears, > > > > I have to order a Rabbit IgG control. What company is making best Rabbit > IgG control, not universal, please. > > > > Thanks in advance, > > Naira > > > > Naira V. Margaryan, D.V.M., Ph.D. > > Research Scientist > > Children's Memorial Research Center > > 2300 Children's Plaza, Box 222 > > Chicago, IL 60614-3363 > > Tel: 773-755-6340 > > Fax: 773-755-6594 > > nmargaryan@childrensmemorial.org > > > > > For Express Mail: > > CMRC, Room C.473 > > 2430 N. Halsted Street > > Chicago, IL 60614-4314 > > > > > > ------------------------------ > > Message: 2 > Date: Fri, 1 Aug 2008 13:07:21 -0400 > From: "Danielson, Keith" > Subject: [Histonet] Alginate beads containing cells > To: > Message-ID: > > <84D11048BB18234D8A56474645E8EC020829EF6F@uphsmbx5.UPHS.PENNHEALTH.PRV> > > Content-Type: text/plain; charset="US-ASCII" > > Hello everyone, > > > > I am requesting information for a graduate student on how to fix and > process alginate beads with embedded tissue culture cells for paraffin > histology. The beads are fixed in 4% paraformaldehyde but seem to turn > white and disintegrate during routine processing. > > > > Thanks very much, > > > > Keith Danielson, PhD > > Supervisor, IHC Lab > > Pennsylvania Hospital > > Philadelphia, PA 19107 > > > > Phone: 215 829-7726 or 829-3696 > > E-mail: keith.danielson@uphs.upenn.edu > > > > > > > > The information contained in this e-mail message is intended only for the > personal and confidential use of the recipient(s) named above. If the reader > of this message is not the intended recipient or an agent responsible for > delivering it to the intended recipient, you are hereby notified that you > have received this document in error and that any review, dissemination, > distribution, or copying of this message is strictly prohibited. If you have > received this communication in error, please notify us immediately by > e-mail, and delete the original message. > > ------------------------------ > > Message: 3 > Date: Fri, 1 Aug 2008 12:23:42 -0500 > From: "Amber McKenzie" > Subject: [Histonet] HT schools > To: > Message-ID: > <03C921A1EAF7F541B16543F6EC6A4B3701D3AEBF@giamail2.Gia.com> > Content-Type: text/plain; charset="US-ASCII" > > Where are all the HT accredited schools and why aren't there more out > there? I've seen the online classes' people can take, but that requires > them to be trained in a lab, as well, for the "hands on" part. So, > actually the supervisor still has to train potential HT's "on the job" > before they can sit for the board exam. Right? > > > > > > > > ------------------------------ > > Message: 4 > Date: Fri, 1 Aug 2008 13:41:00 -0400 > From: christopher.hayden@novartis.com > Subject: [Histonet] Christopher Hayden is nowhere near the lab. > To: histonet@lists.utsouthwestern.edu > Message-ID: > < > OFC6859B0A.C0BE57E7-ON85257498.0061233C-85257498.0061233C@ah.novartis.com> > > Content-Type: text/plain; charset=US-ASCII > > > I will be out of the office starting 08/01/2008 and will not return until > 08/11/2008. > > Good Day Everyone! > > I will be out of the lab from 2 August until 10 August. I'll be back at my > desk on the morning of the 11th. I will have no access to corporate email > during this time. > > If you have an EM-related matter, please contact the Lab Manager, Gregory > Argentieri, at x2-8617 > > Otherwise, I'll get back to you on the 11th or shortly afterwards. > > Thanks! > -CH > > > > > ------------------------------ > > Message: 5 > Date: Fri, 1 Aug 2008 14:13:13 -0400 > From: "Victoria Baker" > Subject: [Histonet] A question for BondMax users > To: "Histo Net list server" > Message-ID: > <4f016b690808011113k4bceed66w3a76c8bb38fa8cb@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Happy Friday everyone! > > In the BondMax detection system there is a "post primary" step that > contains 10% BSA in a Tris buffer. I've tested the protocol excluding > this step and did not have any staining. > > I can't seem to get the answer I'm looking for from the company, so if > anyone can help me I'd really appreciate it. > > Thanks > > Vikki > > > > ------------------------------ > > Message: 6 > Date: Fri, 1 Aug 2008 14:18:43 -0400 > From: "Victoria Baker" > Subject: Re: [Histonet] HT schools > To: "Amber McKenzie" > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > <4f016b690808011118k34ecf6c5r20c4960d00b679c6@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Hi Amber, > > If you go to the NSH website there are schools listed there. I'm an > alumni of the SUNY school at Cobleskill - and I loved it - but it was > also thirty years ago. > > I would check that list and see if you can't make some calls or > e-mails to their co-ordinators. > > Regards, > > Vikki Baker > > > > On 8/1/08, Amber McKenzie wrote: > > Where are all the HT accredited schools and why aren't there more out > > there? I've seen the online classes' people can take, but that requires > > them to be trained in a lab, as well, for the "hands on" part. So, > > actually the supervisor still has to train potential HT's "on the job" > > before they can sit for the board exam. Right? > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 7 > Date: Fri, 1 Aug 2008 13:27:36 -0500 > From: "Beckham, Sharon" > Subject: RE: [Histonet] A question for BondMax users > To: 'Victoria Baker' , Histo Net list server > > Message-ID: > < > BD62CBAC4395B94096109020651BE2EC129EDDCE2F@exchmb-02.stowers-institute.org > > > > Content-Type: text/plain; charset="us-ascii" > > Hi Vikki, > > I don't know if I'm much help on this because I just started testing the > BondMax this week so I'm just in the learning process too. As far as I can > tell the post primary is for blocking, but also works in conjunction with > the polymer which is the following step. It supposedly also enhances > penetration of the polymer, so they both have to be used together. Hope > this helps. > > Sharon > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Baker > Sent: Friday, August 01, 2008 1:13 PM > To: Histo Net list server > Subject: [Histonet] A question for BondMax users > > > Happy Friday everyone! > > In the BondMax detection system there is a "post primary" step that > contains 10% BSA in a Tris buffer. I've tested the protocol excluding this > step and did not have any staining. > > I can't seem to get the answer I'm looking for from the company, so if > anyone can help me I'd really appreciate it. > > Thanks > > Vikki > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 8 > Date: Fri, 1 Aug 2008 13:27:28 -0500 > From: "Dana Brewer" > Subject: [Histonet] Bone Marrow Biopsy Needles > To: > Message-ID: <000601c8f404$619b66c0$0b0a10ac@work.nmh.org> > Content-Type: text/plain; charset="iso-8859-1" > > Does anyone have a replacement source for J Style Needle, -Marrow Loc- Bone > Marrow Biopsy Needles -- no longer manufactured by US BIOPSY -- the > replacement we got is not the same "marrow loc" system our oncologist > prefers. > Thanks for your help > > ------------------------------ > > Message: 9 > Date: Fri, 1 Aug 2008 12:42:14 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] HT schools > To: histonet@lists.utsouthwestern.edu, Amber McKenzie > > Message-ID: <78031.12752.qm@web65716.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Wrong! The advantage of the "on line" or "distance learning" courses is > that they provide the theory on line while you are working at a given > laboratory doing your training (or even as part of your daily work) so there > is no "actual training" to be done by the supervisor. > At this moment NAACLS has 30 HT and 3 HTL accredited programs only, with an > overall capacity of about 300-325 students, and this will not be enough to > take care of all the retiring histotechs. > Costs is one of the reasons why the number of HTs schools is dwindling. > Reni J. > > > --- On Fri, 8/1/08, Amber McKenzie wrote: > > From: Amber McKenzie > Subject: [Histonet] HT schools > To: histonet@lists.utsouthwestern.edu > Date: Friday, August 1, 2008, 1:23 PM > > Where are all the HT accredited schools and why aren't there more out > there? I've seen the online classes' people can take, but that > requires > them to be trained in a lab, as well, for the "hands on" part. So, > actually the supervisor still has to train potential HT's "on the > job" > before they can sit for the board exam. Right? > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 10 > Date: Fri, 1 Aug 2008 15:23:31 -0600 > From: "Gayle Callis" > Subject: [Histonet] processing PFA fixed alginate beads > To: "Histonet Message" > Message-ID: <000801c8f41c$d9196180$6401a8c0@Sunney> > Content-Type: text/plain; charset="iso-8859-1" > > I Googled this subject and brought up several publications using alginate > beads embedded in paraffin. HOwever, the following publication, using > cryotomy, had better results than using paraffin sections. > > Also, Yang C-C, et al. Adapted cyrosectioning method for hydrogels used in > regenerative medicine. J Histotechnology 30(3):185-191, 2007. Linda > Jenkins, on of the co-authors, was a huge part of this, has much experience > with hydrogels. In this publication, they fabricated their own alginate > beads. The reference list is extensive and informative. > > Good Luck > > Gayle M. Callis > HTL/HT/MT(ASCP) > > > > ---------------------------------------------------------------------------- ---- > > > > > > > ------------------------------ > > Message: 11 > Date: Fri, 1 Aug 2008 15:28:53 -0600 > From: "Gayle Callis" > Subject: [Histonet] Methodology for extracting nucleic acids from FFPE > Tissue > To: "Histonet Message" > Message-ID: <000f01c8f41d$990a30f0$6401a8c0@Sunney> > Content-Type: text/plain; charset="iso-8859-1" > > For the person needing a protocol for extracting DNA and/or RNA from breast > (?) tissue, check out QuickExtract FFPE DNA and RNA Extraction Kits from > Epicenter Biotechnologies, www. EpiBio.com > > Phone # is 800 284-8474. > > This information was found in the latest issue of Science ( Life ScienCe > Technologies section) > > Gayle M. Callis > HTL/HT/MT(ASCP) > > > > ------------------------------ > > Message: 12 > Date: Fri, 1 Aug 2008 18:14:56 -0400 > From: "Michael Mihalik" > Subject: RE: [Histonet] Dictation systems > To: , > Message-ID: <001501c8f424$204a2830$60de7890$@com> > Content-Type: text/plain; charset="iso-8859-1" > > Jon, did you ever get an answer to this question? > > I am not as familiar with all the various dictation systems that I would > like to be, but I have used something that may help. > > You can buy a foot pedal that can be 'programmed' to send a hotkey > combination to an external device, typically a PC. > > In my case, I was considering using this setup to enable and disable > Dragon > Naturally Speaking at the gross workstation. > > Michael Mihalik > PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jon Google > Sent: Tuesday, July 29, 2008 3:29 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Dictation systems > > We are in the process of upgrading our LIS. We are looking at moving our > dictation system from our current Dictaphone analog system. We would like > to > use a digital system that can support VOIP. The problem we are running > into > is trying to find one that supports a hands free control that our grossing > pathologists need. > > Does anyone have recommendations on digital systems that support foot > controls? > > Thanks > Jon > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 13 > Date: Sat, 2 Aug 2008 07:19:36 -0500 > From: "Joe Nocito" > Subject: Re: [Histonet] HT schools > To: , , "Amber > McKenzie" > Message-ID: <002c01c8f49a$07995790$e797b348@yourxhtr8hvc4p> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > our one community college program in San Antonio is in jeopardy of closing > because it can't get the required 10 students to make a class. If this > program does close, I will be willing to work with a college or university > with long- distance learning. I have 5 openings in my lab. > > JTT > ----- Original Message ----- > From: "Rene J Buesa" > To: ; "Amber McKenzie" > > Sent: Friday, August 01, 2008 2:42 PM > Subject: Re: [Histonet] HT schools > > > Wrong! The advantage of the "on line" or "distance learning" courses is > that > they provide the theory on line while you are working at a given laboratory > doing your training (or even as part of your daily work) so there is no > "actual training" to be done by the supervisor. > At this moment NAACLS has 30 HT and 3 HTL accredited programs only, with an > overall capacity of about 300-325 students, and this will not be enough to > take care of all the retiring histotechs. > Costs is one of the reasons why the number of HTs schools is dwindling. > Reni J. > > > --- On Fri, 8/1/08, Amber McKenzie wrote: > > From: Amber McKenzie > Subject: [Histonet] HT schools > To: histonet@lists.utsouthwestern.edu > Date: Friday, August 1, 2008, 1:23 PM > > Where are all the HT accredited schools and why aren't there more out > there? I've seen the online classes' people can take, but that > requires > them to be trained in a lab, as well, for the "hands on" part. So, > actually the supervisor still has to train potential HT's "on the > job" > before they can sit for the board exam. Right? > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 57, Issue 2 > *************************************** > ------------------------------ Message: 18 Date: Mon, 4 Aug 2008 10:46:26 -0600 From: "Patsy Ruegg" Subject: RE: [Histonet] HT schools To: "'Cheri Miller'" , , , "'Amber McKenzie'" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Remember that now there is no practical portion of the HT exam, so they are not being tested on hands on experiences anyway. The most difficult problem I have with training people on the job (and I have trained many) is that now they are not prepared to take the exam because they are examined all on theory. I have some really well trained people who can do the work really well, but they have a hard time taking the computer test which they pretty much have to memorize out of books. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Monday, August 04, 2008 7:05 AM To: rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; 'Amber McKenzie' Subject: RE: [Histonet] HT schools Someone has to teach them the "hands on" part of histology. I do not leave this to my staff. I teach / give them the skills they need to perform the practical part of their profession. As their supervisor I am very much involved in their training. I am sure I am not the only one.?? Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, August 01, 2008 2:42 PM To: histonet@lists.utsouthwestern.edu; Amber McKenzie Subject: Re: [Histonet] HT schools Wrong! The advantage of the "on line" or "distance learning" courses is that they provide the theory on line while you are working at a given laboratory doing your training (or even as part of your daily work) so there is no "actual training" to be done by the supervisor. At this moment NAACLS has 30 HT and 3 HTL accredited programs only, with an overall capacity of about 300-325 students, and this will not be enough to take care of all the retiring histotechs. Costs is one of the reasons why the number of HTs schools is dwindling. Reni J. --- On Fri, 8/1/08, Amber McKenzie wrote: From: Amber McKenzie Subject: [Histonet] HT schools To: histonet@lists.utsouthwestern.edu Date: Friday, August 1, 2008, 1:23 PM Where are all the HT accredited schools and why aren't there more out there? I've seen the online classes' people can take, but that requires them to be trained in a lab, as well, for the "hands on" part. So, actually the supervisor still has to train potential HT's "on the job" before they can sit for the board exam. Right? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Mon, 4 Aug 2008 10:46:26 -0600 From: "Patsy Ruegg" Subject: RE: [Histonet] surveillance cameras in the lab To: "'Cheri Miller'" , , Message-ID: Content-Type: text/plain; charset="us-ascii" Come on. In these times of terror concerns I am not sure I would work in a place where I did not feel secure and the use of these devices help in that matter. We built a brand new University of Colorado Health Sciences Center and there are cameras all over the place as well as lock down. If you do not have an access card you cannot get into the labs. This was a pain at first but with all the crazy's we have to worry about out there it now makes me feel better. I just read in the paper this morning about a researcher whose house was bombed by Peta types for doing animal research, and we have had all sorts of disturbances over the years with precious research animals being released, protests, etc. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Monday, August 04, 2008 7:08 AM To: Pathrm35@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] surveillance cameras in the lab That is B.S....I wouldn't work in a lab that used covalence camera's for what ever reason Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathrm35@comcast.net Sent: Monday, August 04, 2008 5:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] surveillance cameras in the lab I was wondering how many techs out there have cameras in their labs, either for security or to monitor employees. I went to work Sunday night and noticed that 4 cameras were installed in the lab over the weekend, with more to come. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Mon, 4 Aug 2008 12:58:44 -0400 From: "William Connor" Subject: [Histonet] QIHC Certification To: Message-ID: <0MKpCa-1KQ3OY3GeG-0004hL@mrelay.perfora.net> Content-Type: text/plain; charset="us-ascii" Greetings, I have just sent in my application in for the QIHC exam. Would anyone out there kindly recommend to me the best study materials to focus on? I have Carson's book and Dako's Immunohistochemical Staining Methods, and am considering the purchase of Diagnostic Immunohistochemistry by Dabbs. I do not work in a hospital setting, but in more of a research setting. Would there be anything I might be missing out on in day to day practice which might be covered on the exam? Thank you in advance for your responses. William P. Connor, HT(ASCP) Senior Histology Technician Histology Tech Services, Inc. This email and its attachments (if any) contain confidential and privileged information. Any dissemination or use of this information by a person other than the intended recipient is unauthorized and may be illegal. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 57, Issue 5 *************************************** From AFeatherstone <@t> KaleidaHealth.Org Mon Aug 4 14:01:03 2008 From: AFeatherstone <@t> KaleidaHealth.Org (Featherstone, Annette) Date: Mon Aug 4 14:01:18 2008 Subject: [Histonet] Washing cassette lids In-Reply-To: References: Message-ID: <16A5E67B2A1F714885DEDC2CB68DD6091D7B36@KALEXMB03.KaleidaHealth.org> We put our lids in the processor cleaning cycle. Annette -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, August 04, 2008 14:54 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 57, Issue 6 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. HT schools (Amber McKenzie) 2. Re: stainless steel bucket (Jackie M O'Connor) 3. Re: surveillance cameras in the lab (Peter Carroll) 4. RE: surveillance cameras in the lab (Baldridge, Lee Ann) 5. RE surveillance cameras and terrorism--baloney (JR R) 6. RE: surveillance cameras in the lab (Bill) 7. stainless steel container (sharon.osborn@comcast.net) 8. RE: surveillance cameras in the lab (Weber, Susan (VHACLE)) 9. RE: surveillance cameras in the lab (Chung, Luong) 10. RE: surveillance cameras in the lab (Bartlett, Jeanine (CDC/CCID/NCZVED)) 11. Re: surveillance cameras in the lab (Peter Carroll) 12. RE: surveillance cameras in the lab (Baldridge, Lee Ann) 13. RE: surveillance cameras in the lab (Bartlett, Jeanine (CDC/CCID/NCZVED)) 14. Re: surveillance cameras in the lab (Peter Carroll) 15. RE: stainless steel bucket (Ford Royer) 16. Re: surveillance cameras in the lab (Markus F. Meyenhofer) 17. Cross polarization and collagen I and III (Liz Chlipala) 18. RE: Cross polarization and collagen I and III (James Watson) 19. Workload Recording (Amy Self) 20. RE: Workload Recording (Martin, Gary) 21. Re: HT schools (Peggy Bisher) 22. RE: p16 on Ventana (Bernice Frederick) ---------------------------------------------------------------------- Message: 1 Date: Mon, 4 Aug 2008 12:08:53 -0500 From: "Amber McKenzie" Subject: [Histonet] HT schools To: Message-ID: <03C921A1EAF7F541B16543F6EC6A4B3701D3B0C3@giamail2.Gia.com> Content-Type: text/plain; charset="iso-8859-1" Even if the online schools teach the theory of Histology, they expect the supervisor/techs to teach the potential HT's how to perform Histology. Even though there is no practical anymore, potential HT's have to be taught the hands on part of the job with OJT. Even though I've heard many people say that there is no more OJT, really there is b/c if these future HT's don't attend an actual HT school where there are classrooms/practice labs full of teachers, then we as working HT's have to teach our future co-workers how to do our job so that we'll have more people to pick from to hire. Does any other profession handle their future employee's like this or is Histology in a category of its own? -----Original Message----- From: Patsy Ruegg [mailto:pruegg@ihctech.net] Sent: Monday, August 04, 2008 11:46 AM To: 'Cheri Miller'; rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; Amber McKenzie Subject: RE: [Histonet] HT schools Remember that now there is no practical portion of the HT exam, so they are not being tested on hands on experiences anyway. The most difficult problem I have with training people on the job (and I have trained many) is that now they are not prepared to take the exam because they are examined all on theory. I have some really well trained people who can do the work really well, but they have a hard time taking the computer test which they pretty much have to memorize out of books. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Monday, August 04, 2008 7:05 AM To: rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; 'Amber McKenzie' Subject: RE: [Histonet] HT schools Someone has to teach them the "hands on" part of histology. I do not leave this to my staff. I teach / give them the skills they need to perform the practical part of their profession. As their supervisor I am very much involved in their training. I am sure I am not the only one.?? Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, August 01, 2008 2:42 PM To: histonet@lists.utsouthwestern.edu; Amber McKenzie Subject: Re: [Histonet] HT schools Wrong! The advantage of the "on line" or "distance learning" courses is that they provide the theory on line while you are working at a given laboratory doing your training (or even as part of your daily work) so there is no "actual training" to be done by the supervisor. At this moment NAACLS has 30 HT and 3 HTL accredited programs only, with an overall capacity of about 300-325 students, and this will not be enough to take care of all the retiring histotechs. Costs is one of the reasons why the number of HTs schools is dwindling. Ren? J. --- On Fri, 8/1/08, Amber McKenzie wrote: From: Amber McKenzie Subject: [Histonet] HT schools To: histonet@lists.utsouthwestern.edu Date: Friday, August 1, 2008, 1:23 PM Where are all the HT accredited schools and why aren't there more out there? I've seen the online classes' people can take, but that requires them to be trained in a lab, as well, for the "hands on" part. So, actually the supervisor still has to train potential HT's "on the job" before they can sit for the board exam. Right? ------------------------------ Message: 2 Date: Mon, 4 Aug 2008 12:13:18 -0500 From: Jackie M O'Connor Subject: Re: [Histonet] stainless steel bucket To: "Amber McKenzie" Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Dog supply store. I purchase stainless steel water buckets for my kennel - they even have handles on them. "Amber McKenzie" Sent by: histonet-bounces@lists.utsouthwestern.edu 08/04/2008 11:06 AM To cc Subject [Histonet] stainless steel bucket Does anyone know where I can purchase a stainless steel basket/bucket to wash my cassette lids in? We use xylene and Liqui-Nox to wash them each day and our plastic buckets eventually end up tearing and breaking over time and I have to constantly replace them, so I'm looking for something more stable like stainless steel. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Mon, 04 Aug 2008 13:13:26 -0400 From: Peter Carroll Subject: Re: [Histonet] surveillance cameras in the lab Cc: histonet@lists.utsouthwestern.edu Message-ID: <489738B6.2020103@umdnj.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed > In these times of terror concerns I am not sure I would work in a place where I did not feel secure and the use of these devices > help in that matter. Wasn't it Ben Franklin who said "He who sacrifices freedom for security deserves neither"? ------------------------------ Message: 4 Date: Mon, 4 Aug 2008 13:14:08 -0400 From: "Baldridge, Lee Ann" Subject: RE: [Histonet] surveillance cameras in the lab To: Patsy Ruegg , "'Cheri Miller'" , "Pathrm35@comcast.net" , "histonet@lists.utsouthwestern.edu" Message-ID: <5E6A94F8037F4F49B738F5B6AD16952210D8F33F95@iu-mssg-mbx09.ads.iu.edu> Content-Type: text/plain; charset="us-ascii" This is my take on the situation: "They who can give up essential liberty to obtain a little temporary safety deserve neither liberty nor safety." -Ben Franklin It doesn't matter how many cameras there are, if someone wants to do something they will find a way. I came in this morning to find someone had stolen my computer speakers and one of our printers from our AutoStainers. Lee Ann Baldridge IUSM Indpls., IN. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Monday, August 04, 2008 12:46 PM To: 'Cheri Miller'; Pathrm35@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] surveillance cameras in the lab Come on. In these times of terror concerns I am not sure I would work in a place where I did not feel secure and the use of these devices help in that matter. We built a brand new University of Colorado Health Sciences Center and there are cameras all over the place as well as lock down. If you do not have an access card you cannot get into the labs. This was a pain at first but with all the crazy's we have to worry about out there it now makes me feel better. I just read in the paper this morning about a researcher whose house was bombed by Peta types for doing animal research, and we have had all sorts of disturbances over the years with precious research animals being released, protests, etc. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Monday, August 04, 2008 7:08 AM To: Pathrm35@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] surveillance cameras in the lab That is B.S....I wouldn't work in a lab that used covalence camera's for what ever reason Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathrm35@comcast.net Sent: Monday, August 04, 2008 5:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] surveillance cameras in the lab I was wondering how many techs out there have cameras in their labs, either for security or to monitor employees. I went to work Sunday night and noticed that 4 cameras were installed in the lab over the weekend, with more to come. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Mon, 4 Aug 2008 10:14:43 -0700 From: JR R Subject: [Histonet] RE surveillance cameras and terrorism--baloney To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" I work in a secure building--you need proximty cards to get in, use the elevators, etc. But cameras in the lab seem rather much. If you have a Union, I woud consider make a greivance out of it--unsafe and hostile work environment, kind of thing. Jerry Ricks Research Scientist University of Washington Department of Pathology.> From: pruegg@ihctech.net> To: cmiller@physlab.com; Pathrm35@comcast.net; histonet@lists.utsouthwestern.edu> Date: Mon, 4 Aug 2008 10:46:26 -0600> Subject: RE: [Histonet] surveillance cameras in the lab> CC: > > Come on.> In these times of terror concerns I am not sure I would work in a place> where I did not feel secure and the use of these devices help in that> matter.> We built a brand new University of Colorado Health Sciences Center and there> are cameras all over the place as well as lock down. If you do not have an> access card you cannot get into the labs. This was a pain at first but with> all the crazy's we have to worry about out there it now makes me feel> better. > I just read in the paper this morning about a researcher whose house was> bombed by Peta types for doing animal research, and we have had all sorts of> disturbances over the years with precious research animals being released,> protests, etc.> Patsy> > Patsy Ruegg, HT(ASCP)QIHC > IHCtech> 12635 Montview Blvd. #215> Aurora, CO 80045> 720-859-4060> fax 720-859-4110> pruegg@ihctech.net> www.ihctech.net> www.ihcrg.org > > > > -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller> Sent: Monday, August 04, 2008 7:08 AM> To: Pathrm35@comcast.net; histonet@lists.utsouthwestern.edu> Subject: RE: [Histonet] surveillance cameras in the lab> > That is B.S....I wouldn't work in a lab that used covalence camera's for> what ever reason> > Cheryl Miller HT (ASCP)> Histology Supervisor> Physicians Laboratory,P.C.> Omaha, Ne. > 402 738 5052> > -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of> Pathrm35@comcast.net> Sent: Monday, August 04, 2008 5:21 AM> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] surveillance cameras in the lab> > I was wondering how many techs ou t there have cameras in their labs, either> for security or to monitor employees. I went to work Sunday night and> noticed that 4 cameras were installed in the lab over the weekend, with more> to come.> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If> you are not the addressee intended / indicated or agent responsible for> delivering it to the addressee, you are hereby notified that you are in> possession of confidential and privileged information. Any dissemination,> distribution, or copying of this e-mail is strictly prohibited. If you have> received this message in error, please notify the sender immediately and> delete this email from your system.> > > > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If> you are not the addressee intended / indicated or agent responsible for> delivering it to the addressee, you are hereby notified that you are in> possession of confidential and privileged information. Any dissemination,> distribution, or copying of this e-mail is strictly prohibited. If you have> received this message in error, please notify the sender immediately and> delete this email from your system.> > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Get more from your digital life. Find out how. http://www.windowslive.com/default.html?ocid=TXT_TAGLM_WL_Home2_082008 ------------------------------ Message: 6 Date: Mon, 4 Aug 2008 12:18:56 -0500 From: Bill Subject: RE: [Histonet] surveillance cameras in the lab To: Message-ID: Content-Type: text/plain; charset="us-ascii" At 10:46 AM -0600 8/4/08, Patsy Ruegg wrote: >In these times of terror concerns I am not sure I would work in a place >where I did not feel secure and the use of these devices help in that >matter. "He who sacrifices freedom for security deserves neither." -Benjamin Franklin BB ------------------------------ Message: 7 Date: Mon, 04 Aug 2008 17:20:11 +0000 From: sharon.osborn@comcast.net Subject: [Histonet] stainless steel container To: histonet@lists.utsouthwestern.edu Message-ID: <080420081720.9819.48973A4B0002BC1F0000265B2215586394029D010D9C01D202019D0E089C@comcast.net> Amber, consider looking in a kitchen ware department for stainless steel mixing bowls or tall cannister sets with the appropriate sized containers that you want. Also, commercial kitchen/restuarant suppliers will have large stainless steel containers as well as varying sizes. If you have a Smart and Final, they sometimes have cooking utensils. sharon osborn, BS,HT(ASCP)CT LabVision ThermoFisher Scientific Fremont, CA Message: 11 Date: Mon, 4 Aug 2008 11:06:26 -0500 From: "Amber McKenzie" Subject: [Histonet] stainless steel bucket To: Message-ID: <03C921A1EAF7F541B16543F6EC6A4B3701D3B09F@giamail2.Gia.com> Content-Type: text/plain; charset="US-ASCII" Does anyone know where I can purchase a stainless steel basket/bucket to wash my cassette lids in? We use xylene and Liqui-Nox to wash them each day and our plastic buckets eventually end up tearing and breaking over time and I have to constantly replace them, so I'm looking for something more stable like stainless steel. ------------------------------ Message: 8 Date: Mon, 4 Aug 2008 13:25:20 -0400 From: "Weber, Susan (VHACLE)" Subject: RE: [Histonet] surveillance cameras in the lab To: "Patsy Ruegg" , "Cheri Miller" , , Message-ID: <16C83872A53F4346AA9C3A18E3A3AAB903F76E16@VHAV10MSGA1.v10.med.va.gov> Content-Type: text/plain; charset="us-ascii" Yes. Security is of the highest priority for these matters, however, were the cameras in the "public" places? Entrances and exits which need to be and usually are monitored I would understand. We also have lockdown procedures and cameras in the "public" areas and key card accesses which, yes are a pain, but necessary, especially in these times. It is also quite clear that you are under video surveillance (there are signs POSTED everywhere!) Four cameras, which seem to be "monitoring" the workforce I do have a problem with. Silently installing them on a weekend without benefit of explanation, I have a problem with. Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Monday, August 04, 2008 12:46 PM To: 'Cheri Miller'; Pathrm35@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] surveillance cameras in the lab Come on. In these times of terror concerns I am not sure I would work in a place where I did not feel secure and the use of these devices help in that matter. We built a brand new University of Colorado Health Sciences Center and there are cameras all over the place as well as lock down. If you do not have an access card you cannot get into the labs. This was a pain at first but with all the crazy's we have to worry about out there it now makes me feel better. I just read in the paper this morning about a researcher whose house was bombed by Peta types for doing animal research, and we have had all sorts of disturbances over the years with precious research animals being released, protests, etc. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Monday, August 04, 2008 7:08 AM To: Pathrm35@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] surveillance cameras in the lab That is B.S....I wouldn't work in a lab that used covalence camera's for what ever reason Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathrm35@comcast.net Sent: Monday, August 04, 2008 5:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] surveillance cameras in the lab I was wondering how many techs out there have cameras in their labs, either for security or to monitor employees. I went to work Sunday night and noticed that 4 cameras were installed in the lab over the weekend, with more to come. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 4 Aug 2008 13:27:31 -0400 From: "Chung, Luong" Subject: RE: [Histonet] surveillance cameras in the lab To: "Baldridge, Lee Ann" , "Patsy Ruegg" , "Cheri Miller" , , Message-ID: <86691924ECCDBE4F82CCAB553424607101DC696C@exchange2.phoebe.com> Content-Type: text/plain; charset="iso-8859-1" You should call up one of the Television networks and make a "reality show" out of it. Just a thought. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Baldridge, Lee Ann Sent: Monday, August 04, 2008 1:14 PM To: Patsy Ruegg; 'Cheri Miller'; Pathrm35@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] surveillance cameras in the lab This is my take on the situation: "They who can give up essential liberty to obtain a little temporary safety deserve neither liberty nor safety." -Ben Franklin It doesn't matter how many cameras there are, if someone wants to do something they will find a way. I came in this morning to find someone had stolen my computer speakers and one of our printers from our AutoStainers. Lee Ann Baldridge IUSM Indpls., IN. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Monday, August 04, 2008 12:46 PM To: 'Cheri Miller'; Pathrm35@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] surveillance cameras in the lab Come on. In these times of terror concerns I am not sure I would work in a place where I did not feel secure and the use of these devices help in that matter. We built a brand new University of Colorado Health Sciences Center and there are cameras all over the place as well as lock down. If you do not have an access card you cannot get into the labs. This was a pain at first but with all the crazy's we have to worry about out there it now makes me feel better. I just read in the paper this morning about a researcher whose house was bombed by Peta types for doing animal research, and we have had all sorts of disturbances over the years with precious research animals being released, protests, etc. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Monday, August 04, 2008 7:08 AM To: Pathrm35@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] surveillance cameras in the lab That is B.S....I wouldn't work in a lab that used covalence camera's for what ever reason Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pathrm35@comcast.net Sent: Monday, August 04, 2008 5:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] surveillance cameras in the lab I was wondering how many techs out there have cameras in their labs, either for security or to monitor employees. I went to work Sunday night and noticed that 4 cameras were installed in the lab over the weekend, with more to come. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- Disclaimer: The HIPAA Final Privacy Rule requires covered entities to safeguard certain Protected Health Information (PHI) related to a person's healthcare. Information being faxed to you may include PHI after appropriate authorization from the patient or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain PHI in a safe and secure manner. You may not re-disclose without additional patient consent or as required by law. Unauthorized re-disclosure or failure to safeguard PHI could subject you to penalties described in federal (HIPAA) and state law. If you the reader of this message are not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, please notify us immediately and destroy the related message. ------------------------------ Message: 10 Date: Mon, 4 Aug 2008 13:27:47 -0400 From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" Subject: RE: [Histonet] surveillance cameras in the lab To: Bill , histonet@lists.utsouthwestern.edu Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A7F23E2A@LTA3VS011.ees.hhs.gov> Content-Type: text/plain; charset=us-ascii I don't think Ben Franklin's quote applies here: we are talking about a place of employment not your home. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bill Sent: Monday, August 04, 2008 1:19 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] surveillance cameras in the lab At 10:46 AM -0600 8/4/08, Patsy Ruegg wrote: >In these times of terror concerns I am not sure I would work in a place >where I did not feel secure and the use of these devices help in that >matter. "He who sacrifices freedom for security deserves neither." -Benjamin Franklin BB _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Mon, 04 Aug 2008 13:33:11 -0400 From: Peter Carroll Subject: Re: [Histonet] surveillance cameras in the lab Cc: histonet@lists.utsouthwestern.edu Message-ID: <48973D57.7070901@umdnj.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed > I don't think Ben Franklin's quote applies here: we are talking about a place of employment not your home. though we're quickly approaching content off-topic for this list, id like to raise the rhetorical question: civil-liberties dont apply in the workplace? i dont know about y'all, but i spend as much time at work as i do at home, by and large, and would be rather bothered if cameras appeared with no justification/explanation. just as i have nothing to hide from said cameras, they have no reason to watch me, by default. ------------------------------ Message: 12 Date: Mon, 4 Aug 2008 13:36:24 -0400 From: "Baldridge, Lee Ann" Subject: RE: [Histonet] surveillance cameras in the lab To: "Bartlett, Jeanine (CDC/CCID/NCZVED)" , Bill , "histonet@lists.utsouthwestern.edu" Message-ID: <5E6A94F8037F4F49B738F5B6AD16952210D8F33F99@iu-mssg-mbx09.ads.iu.edu> Content-Type: text/plain; charset="us-ascii" Oh but it does. Our liberties are eroded away a little bit at a time. Where does it stop? Lee Ann Baldridge IUSM Indpls.,IN. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Monday, August 04, 2008 1:28 PM To: Bill; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] surveillance cameras in the lab I don't think Ben Franklin's quote applies here: we are talking about a place of employment not your home. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bill Sent: Monday, August 04, 2008 1:19 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] surveillance cameras in the lab At 10:46 AM -0600 8/4/08, Patsy Ruegg wrote: >In these times of terror concerns I am not sure I would work in a place >where I did not feel secure and the use of these devices help in that >matter. "He who sacrifices freedom for security deserves neither." -Benjamin Franklin BB _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Mon, 4 Aug 2008 13:39:40 -0400 From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" Subject: RE: [Histonet] surveillance cameras in the lab To: "Peter Carroll" Cc: histonet@lists.utsouthwestern.edu Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A7F23E2E@LTA3VS011.ees.hhs.gov> Content-Type: text/plain; charset=us-ascii I agree they should have been notified and all that but my point is that if I do not like the policies where I work I am free to go somewhere else. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Peter Carroll Sent: Monday, August 04, 2008 1:33 PM Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] surveillance cameras in the lab > I don't think Ben Franklin's quote applies here: we are talking about a place of employment not your home. though we're quickly approaching content off-topic for this list, id like to raise the rhetorical question: civil-liberties dont apply in the workplace? i dont know about y'all, but i spend as much time at work as i do at home, by and large, and would be rather bothered if cameras appeared with no justification/explanation. just as i have nothing to hide from said cameras, they have no reason to watch me, by default. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Mon, 04 Aug 2008 13:42:05 -0400 From: Peter Carroll Subject: Re: [Histonet] surveillance cameras in the lab Cc: histonet@lists.utsouthwestern.edu Message-ID: <48973F6D.1030503@umdnj.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed > if I do not like the policies where I work I am free to go somewhere else. thats a useful option... until the point where every viable place you could work is subject to the same draconian policy, cameras and lack of explanation. ------------------------------ Message: 15 Date: Mon, 4 Aug 2008 12:45:23 -0500 From: "Ford Royer" Subject: RE: [Histonet] stainless steel bucket To: "'Amber McKenzie'" , Message-ID: <008101c8f659$df5aa100$7701a80a@Ford> Content-Type: text/plain; charset="iso-8859-1" Not sure if this is what you're looking for, but we carry stainless steel buckets/beakers/storage containers. Link: http://stores.implex.net/minnesotamedical/index.php?main_page=index&cPath=56 _193&zenid=b780c44693573ef63f1b26b8b8bcb664 Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL:? 612-839-1046 Phone:? 763-542-8725 Fax:? 763-546-4830 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Monday, August 04, 2008 11:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] stainless steel bucket Does anyone know where I can purchase a stainless steel basket/bucket to wash my cassette lids in? We use xylene and Liqui-Nox to wash them each day and our plastic buckets eventually end up tearing and breaking over time and I have to constantly replace them, so I'm looking for something more stable like stainless steel. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Mon, 4 Aug 2008 13:55:23 -0400 From: "Markus F. Meyenhofer" Subject: Re: [Histonet] surveillance cameras in the lab To: "Peter Carroll" Cc: histonet@lists.utsouthwestern.edu Message-ID: <059201c8f65b$45dce360$0f893cd1@DJ4VDH31> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=response Well said (Ben's words) Thank you! ----- Original Message ----- From: "Peter Carroll" Cc: Sent: Monday, August 04, 2008 1:13 PM Subject: Re: [Histonet] surveillance cameras in the lab > > In these times of terror concerns I am not sure I would work in a > place where I did not feel secure and the use of these devices > > help in that matter. > > Wasn't it Ben Franklin who said "He who sacrifices freedom for security > deserves neither"? > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 17 Date: Mon, 4 Aug 2008 11:59:26 -0600 From: "Liz Chlipala" Subject: [Histonet] Cross polarization and collagen I and III To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hello again If anyone remembers my post late last week on cross polarization the reason why I asked that is that I have a paper of a technique that used cross polarization and picrosirius stained slides to differenciate collagen I fibers from collagen III fibers. In the paper they basically stated the the thicker collagen I fibers looked orange-red and the thinner collagen III fibers looked green. I have not heard of this before and was a bit confused, I thought that picrosirus just stained for collagen, so I initally thought that maybe cross polarization is different than routine polarization. I have since found out that they are one in the same. My question now is there anyone out there familar with using picrosirus to differenciate collagen III from collagen I and can you do that? Thanks in advance PS - I do have the paper if anyone is intersted in looking at it. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 ------------------------------ Message: 18 Date: Mon, 4 Aug 2008 11:27:20 -0700 From: "James Watson" Subject: RE: [Histonet] Cross polarization and collagen I and III To: "Liz Chlipala" , Message-ID: Content-Type: text/plain; charset="us-ascii" Liz, I have watching this thread to see if someone mentions a rotating stage. Having serviced microscopes in the past, I know that there are polarizing microscopes that are set up with a rotating polarizer below the condenser and a stationary analyzer (same as a Polarizer but called an analyzer because it is above the specimen) above the objectives. Then once you have aligned the polarizer and analyzer to get the polarized light you can rotate the stage of the microscope to see the different colors depending on the alignment of the crystal or fiber. Picrosirus red will stain other tissue elements and the conformation of what is stained is collagen is with polarization (Histological & Histochemical Methods J.A. Kiernan pg. 150). This is a good reference on this technique. Please send me a copy of this paper. Thank you James Watson HT ASCP Facilities Manager of Histology GNF Genomics Institute of the Novartis Research Foundation Tel 858-332-4647 Fax 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Monday, August 04, 2008 10:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cross polarization and collagen I and III Hello again If anyone remembers my post late last week on cross polarization the reason why I asked that is that I have a paper of a technique that used cross polarization and picrosirius stained slides to differenciate collagen I fibers from collagen III fibers. In the paper they basically stated the the thicker collagen I fibers looked orange-red and the thinner collagen III fibers looked green. I have not heard of this before and was a bit confused, I thought that picrosirus just stained for collagen, so I initally thought that maybe cross polarization is different than routine polarization. I have since found out that they are one in the same. My question now is there anyone out there familar with using picrosirus to differenciate collagen III from collagen I and can you do that? Thanks in advance PS - I do have the paper if anyone is intersted in looking at it. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Mon, 4 Aug 2008 14:39:45 -0400 From: "Amy Self" Subject: [Histonet] Workload Recording To: Message-ID: Content-Type: text/plain; charset="us-ascii" Histonetters, Does anyone have any info and/or charts on workload recording in histology that they would be willing to share with me? Thanks in advance, Amy Georgetown Hospital System Dept. of Pathology NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ------------------------------ Message: 20 Date: Mon, 4 Aug 2008 11:43:57 -0700 From: "Martin, Gary" Subject: RE: [Histonet] Workload Recording To: "Amy Self" , Message-ID: <6ED9D4252F278841A0593D3D788AF24C02FC5E54@mailsvr.MARSHMED.local> Content-Type: text/plain; charset="us-ascii" Forget logs ... just get some cameras :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Monday, August 04, 2008 11:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Workload Recording Histonetters, Does anyone have any info and/or charts on workload recording in histology that they would be willing to share with me? Thanks in advance, Amy Georgetown Hospital System Dept. of Pathology NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Mon, 04 Aug 2008 14:44:49 -0400 From: Peggy Bisher Subject: Re: [Histonet] HT schools To: Amber McKenzie , Message-ID: Content-Type: text/plain; charset="ISO-8859-1" I would say that teaching Electron Microscopy is the same. There are a few schools (two, I believe) that offer an official degree (it's an Associate Degree) in Electron Microscopy, but most of my learning has been on the job and that is how I work with my students - one on one. Here at Princeton we offer a class in electron optics, but it is just theory. My first job was at the National Institutes of Health and my boss there had a degree in theoretical physics. He gave me a few books and I taught myself, at least in the beginning. He knew the instrument, but nothing about sample prep. The Microscopy Society of America does offer a Electron Microscopist Certification for those who want to have this. But I have been doing EM since 1980, so never felt the need to be certified. Margaret E. Bisher Electron Microscopy & Histology Core Facility Manager Department of Molecular Biology Princeton University Moffett Laboratory, Room 113 Princeton, New Jersey Office: (609) 258-7026 Fax: (609) 258-8468 mbisher@princeton.edu On 8/4/08 1:08 PM, "Amber McKenzie" wrote: > Even if the online schools teach the theory of Histology, they expect the > supervisor/techs to teach the potential HT's how to perform Histology. Even > though there is no practical anymore, potential HT's have to be taught the > hands on part of the job with OJT. Even though I've heard many people say > that there is no more OJT, really there is b/c if these future HT's don't > attend an actual HT school where there are classrooms/practice labs full of > teachers, then we as working HT's have to teach our future co-workers how to > do our job so that we'll have more people to pick from to hire. Does any > other profession handle their future employee's like this or is Histology in a > category of its own? > > > > -----Original Message----- > From: Patsy Ruegg [mailto:pruegg@ihctech.net] > Sent: Monday, August 04, 2008 11:46 AM > To: 'Cheri Miller'; rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; > Amber McKenzie > Subject: RE: [Histonet] HT schools > > > > Remember that now there is no practical portion of the HT exam, so they are > > not being tested on hands on experiences anyway. The most difficult problem > > I have with training people on the job (and I have trained many) is that now > > they are not prepared to take the exam because they are examined all on > > theory. I have some really well trained people who can do the work really > > well, but they have a hard time taking the computer test which they pretty > > much have to memorize out of books. > > Patsy > > > > Patsy Ruegg, HT(ASCP)QIHC > > IHCtech > > 12635 Montview Blvd. #215 > > Aurora, CO 80045 > > 720-859-4060 > > fax 720-859-4110 > > pruegg@ihctech.net > > www.ihctech.net > > www.ihcrg.org > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller > > Sent: Monday, August 04, 2008 7:05 AM > > To: rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; 'Amber McKenzie' > > Subject: RE: [Histonet] HT schools > > > > Someone has to teach them the "hands on" part of histology. I do not leave > > this to my staff. I teach / give them the skills they need to perform the > > practical part of their profession. As their supervisor I am very much > > involved in their training. I am sure I am not the only one.?? > > > > Cheryl Miller HT (ASCP) > > Histology Supervisor > > Physicians Laboratory,P.C. > > Omaha, Ne. > > 402 738 5052 > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > > Sent: Friday, August 01, 2008 2:42 PM > > To: histonet@lists.utsouthwestern.edu; Amber McKenzie > > Subject: Re: [Histonet] HT schools > > > > Wrong! The advantage of the "on line" or "distance learning" courses is that > > they provide the theory on line while you are working at a given laboratory > > doing your training (or even as part of your daily work) so there is no > > "actual training" to be done by the supervisor. > > At this moment NAACLS has 30 HT and 3 HTL accredited programs only, with an > > overall capacity of about 300-325 students, and this will not be enough to > > take care of all the retiring histotechs. > > Costs is one of the reasons why the number of HTs schools is dwindling. > > Ren? J. > > > > > > --- On Fri, 8/1/08, Amber McKenzie wrote: > > > > From: Amber McKenzie > > Subject: [Histonet] HT schools > > To: histonet@lists.utsouthwestern.edu > > Date: Friday, August 1, 2008, 1:23 PM > > > > Where are all the HT accredited schools and why aren't there more out > > there? I've seen the online classes' people can take, but that > > requires > > them to be trained in a lab, as well, for the "hands on" part. So, > > actually the supervisor still has to train potential HT's "on the > > job" > > before they can sit for the board exam. Right? > > > > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 22 Date: Mon, 4 Aug 2008 13:50:48 -0500 From: "Bernice Frederick" Subject: RE: [Histonet] p16 on Ventana To: "'Sharon.Davis-Devine'" , Message-ID: <000001c8f663$05e9c220$d00f7ca5@lurie.northwestern.edu> Content-Type: text/plain; charset="us-ascii" Both Labvision and novacastra (Leica vision biosystems) sell it. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Monday, August 04, 2008 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 on Ventana Hey Histonetters! Is anyone out there in histoworld running p16 on the Ventana system? If so, can you provide us with your protocol and where you are ordering it from? We are aware that MTM is presently the only vendor for p16 but it is rather pricey and are wondering if there are any alternatives available out there. Once again thank you so much for your help. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 57, Issue 6 *************************************** 2007 Best Places to Work Finalist Visit our careers page at www.kaleidahealth.org/careers CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From jacobc <@t> mmc.org Mon Aug 4 14:13:58 2008 From: jacobc <@t> mmc.org (Christine Jacobs) Date: Mon Aug 4 14:14:14 2008 Subject: [Histonet] p16 on Ventana Message-ID: <20080804T151358Z_C07000110000@mmc.org> Yes there are other vendors out there but MTM is the only one with the legal authority to sell this as an IVD. If you are not concerned about using RUO's in your lab, the alternatives are an option. Chris Jacobs, HT(ASCP), QIHC NorDx Laboratory Scarborough, ME CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the use of the intended recipient(s) only and may contain information that is privileged, confidential, and prohibited from unauthorized disclosure under applicable law. If you are not the intended recipient of this message, any dissemination, distribution, or copying of this message is strictly prohibited. If you received this message in error, please notify the sender by reply email and destroy all copies of the original message and attachments. From mpence <@t> grhs.net Mon Aug 4 14:19:12 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Aug 4 14:19:17 2008 Subject: [Histonet] surveillance cameras in the lab In-Reply-To: <059201c8f65b$45dce360$0f893cd1@DJ4VDH31> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A38C1@IS-E2K3.grhs.net> And maybe they are not just watching on their cameras, but also monitoring this entire thread on your computer. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Markus F. Meyenhofer Sent: Monday, August 04, 2008 12:55 PM To: Peter Carroll Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] surveillance cameras in the lab Well said (Ben's words) Thank you! ----- Original Message ----- From: "Peter Carroll" Cc: Sent: Monday, August 04, 2008 1:13 PM Subject: Re: [Histonet] surveillance cameras in the lab > > In these times of terror concerns I am not sure I would work in a > place where I did not feel secure and the use of these devices > > help in that matter. > > Wasn't it Ben Franklin who said "He who sacrifices freedom for > security > deserves neither"? > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Mon Aug 4 14:32:53 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Mon Aug 4 14:32:57 2008 Subject: [Histonet] Re: stainless steel bucket Message-ID: Amber McKenzie asks: Does anyone know where I can purchase a stainless steel basket/bucket to wash my cassette lids in? We use xylene and Liqui-Nox to wash them each day.... Several months ago I spent US$20 on a 7 x 7 inch, 1-gallon stainless steel bucket with a loose-fitting lid. This item, made in India, is offered by Gardeners Supply Company (gardeners.com - I have no connection with them) to store kitchen waste in until it's time to take it out to the compost heap. It replaced a truly gross plastic container I'd had for several years. (In my kitchen not a lettuce leaf falleth but it goeth to the composter. You should see my tomatoes.) According to its online MSDS, Liqui-Nox is a heavy duty detergent, sodium dodecylbenzenesulfonate, which I wouldn't think would corrode stainless steel. Bob Richmond Samurai Pathologist Knoxville TN From rosenfeldtek <@t> hotmail.com Mon Aug 4 15:05:48 2008 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Mon Aug 4 15:05:54 2008 Subject: [Histonet] Cameras in the Lab-Possible Abuses Message-ID: Am I the only person who can think of abuses of such monitoring? Jerry Ricks _________________________________________________________________ Your PC, mobile phone, and online services work together like never before. http://clk.atdmt.com/MRT/go/108587394/direct/01/ From talulahgosh <@t> gmail.com Mon Aug 4 15:36:08 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Mon Aug 4 15:36:12 2008 Subject: [Histonet] surveillance cameras in the lab In-Reply-To: <86691924ECCDBE4F82CCAB553424607101DC696C@exchange2.phoebe.com> References: <5E6A94F8037F4F49B738F5B6AD16952210D8F33F95@iu-mssg-mbx09.ads.iu.edu> <86691924ECCDBE4F82CCAB553424607101DC696C@exchange2.phoebe.com> Message-ID: Wow, the reality show from a lab point of view. We should come up with a good name and script and sell that baby! Emily On Mon, Aug 4, 2008 at 1:27 PM, Chung, Luong wrote: > You should call up one of the Television networks and make a "reality show" > out of it. > > Just a thought. > > > > -- An overcivilized people grow complacent and careless and leave the door open for a tribe of fanatical savages, through a mixture of luck, treachery, and the foulest inhumanity, to usurp their place for a few years. -Richard Adams, "Shardik", 1974 From awatanabe <@t> tgen.org Mon Aug 4 15:37:15 2008 From: awatanabe <@t> tgen.org (Aprill Watanabe) Date: Mon Aug 4 15:37:20 2008 Subject: [Histonet] p53 staining pattern Message-ID: I have an intellectual question for you all. I have recently stained a colon tumor with p53 from Novocastra and got clear areas of positive and negative staining for no visible reason (i.e. Normal tissue vs. tumor tissue). It looks like a fixation issue but in some areas there will be no staining then a small group of cells (like an island) will stain. I?m not sure how to check if this is a biological function or if this is caused by something pre-analytical. Can anyone help? Aprill Watanabe, B.S. Research Associate Integrated Cancer Genomics Division Tissue Microarray Center (TMA) Translational Genomics Research Institute (TGen) main: 602-343-8822 Fax: 602-343-8840 awatanabe@tgen.org www.tgen.org From JPCOLEMA <@t> sentara.com Mon Aug 4 15:57:56 2008 From: JPCOLEMA <@t> sentara.com (JOHN P COLEMAN) Date: Mon Aug 4 15:58:20 2008 Subject: [Histonet] Cameras, Histo schools Message-ID: I partnered with a university and 2 other hospital systems and we were unsuccessful recruiting students for an HT program. In the 3 years the program has been running we have had a total of 4 students. The university will probably pull funding from the program and kill it next semester. We installed cameras temporarily to perform a LEAN analysis. We told the employees, and the cameras were removed at the end of the project. No one liked them however. John P.J. Coleman HT(ASCP)QIHC Clinical Specialist Anatomic Pathology Sentara Laboratory Services Cell/Voicemail(757)335-2159 pager: (757)456-6695 office:-(757)388-3295 From akbitting <@t> geisinger.edu Mon Aug 4 16:17:10 2008 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Mon Aug 4 16:17:22 2008 Subject: [Histonet] surveillance cameras in the lab In-Reply-To: <48973D57.7070901@umdnj.edu> References: <200808041246.1kq3cU5gg3Nl3oJ1@wanamaker.mail.atl.earthlink.net> <1CE1847DFEA0A647B1CCDE4108EA60A7F23E2A@LTA3VS011.ees.hhs.gov> <48973D57.7070901@umdnj.edu> Message-ID: <48973996.2B7F.00C9.0@geisinger.edu> Unfortunately in todays world, thanks to the minority of goofballs-- the majority suffers too. We have a lot to learn from the animal kingdom. The strong eat the goofballs. >>> Peter Carroll 8/4/2008 1:33 PM >>> > I don't think Ben Franklin's quote applies here: we are talking about a place of employment not your home. though we're quickly approaching content off-topic for this list, id like to raise the rhetorical question: civil-liberties dont apply in the workplace? i dont know about y'all, but i spend as much time at work as i do at home, by and large, and would be rather bothered if cameras appeared with no justification/explanation. just as i have nothing to hide from said cameras, they have no reason to watch me, by default. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. 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From pruegg <@t> ihctech.net Mon Aug 4 17:43:47 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Aug 4 17:43:32 2008 Subject: [Histonet] jcoleman IHCRG Membership Application Form Message-ID: Verify NSH membership please Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org _____ From: webmaster@neo.agsci.colostate.edu [mailto:webmaster@neo.agsci.colostate.edu] Sent: Monday, August 04, 2008 2:45 PM To: patsy.ruegg@gmail.com Subject: IHCRG Membership Application Form _____ New_Application: Yes Last_Name: Coleman First_Name: John NSH_Member: Yes Employer: Sentara Healthcare Address: 600 Gresham Drive City: Norfolk State: VA Zip: 23507 Province: Country: USA Phone: (757) 335-2159 Ext: Fax: (757) 388- 3799 Email: jpcolema@sentara.com Surgical_Pathology: Yes Hematopathology: Yes Orthopedic: Veterinary: Immunology: Plastics: Research: GU Paraffin: Yes Frozen: Yes Cytospins: Smears_Touch_Preps: Yes Plastics_Sample: Sample_Other: PAP: APAAP: ABC_HRP: ABC_AP: SA_HRP: Yes SA_AP: Yes IF: Yes IHC_Other: ISH: Yes Gels: PCR: Mol_Other: Automated_IHC: Yes Company: Biogenex IHC_Qualification: Yes Date_Taken_Exam: 1997? Like_To_Take_Exam: No Expected_Date: Can_Provide_Tissues: Yes Tissues_Provided: Pneumocystis carinii, Hepatitis C, many others Need_Tissues: No Tissue_Needed: Remote Name: 163.230.7.6 HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1; .NET CLR 1.1.4322) Date: 08/04/2008 Time: 02:44 PM Other_Tech Rapid Microwave processing From jnocito <@t> satx.rr.com Mon Aug 4 17:59:40 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Aug 4 17:59:45 2008 Subject: [Histonet] surveillance cameras in the lab References: Message-ID: <005a01c8f685$c6ca9e70$e797b348@yourxhtr8hvc4p> knuckle dragger? ----- Original Message ----- From: "Dawson, Glen" To: Sent: Monday, August 04, 2008 7:51 AM Subject: RE: [Histonet] surveillance cameras in the lab I would suggest that your institution take the $$ that they will flush on the surveillance equipment and on the knuckle dragger that sits in front of the screens to spy on the techs & give it to the histo-staff as a bonus. This would have a much more positive effect on workflow than the "1984" approach. Another lab should be considered as I doubt that it will stop there. Best of Luck, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Pathrm35@comcast.net Sent: Monday, August 04, 2008 5:58 AM To: Joe Nocito; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] surveillance cameras in the lab No problems with theft or problems with employees. Maybe to monitor the workload? Can't seem to get an answer. -------------- Original message -------------- From: "Joe Nocito" > has theft been a problem? If not, this is the first case of big brother in > the lab that I've heard of. Pretty sad if you ask me. Oh yeah, you did > ask. > Stuff like this just curls my toenails. > > JTT > ----- Original Message ----- > From: > To: > Sent: Monday, August 04, 2008 5:21 AM > Subject: [Histonet] surveillance cameras in the lab > > > >I was wondering how many techs out there have cameras in their labs, > >either > >for security or to monitor employees. I went to work Sunday night and > >noticed that 4 cameras were installed in the lab over the weekend, with > >more to come. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Mon Aug 4 18:00:33 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Aug 4 18:00:39 2008 Subject: [Histonet] surveillance cameras in the lab References: <080420081021.7972.4896D816000E927500001F242212020784CACC039D089B0EAF@comcast.net> Message-ID: <006c01c8f685$e6f33180$e797b348@yourxhtr8hvc4p> and it's not even Friday yet!!!!! ----- Original Message ----- From: "Emily Sours" To: ; Sent: Monday, August 04, 2008 8:13 AM Subject: Re: [Histonet] surveillance cameras in the lab > That's really creepy. You should stage a musical extravaganza for > whomever > watches it. > Science: The Musical! > Featuring the Busby Berkley rolling chair dance! PPE required (heigh-o!) > > Emily > -- > An overcivilized people grow complacent and careless and leave the door > open > for a tribe of fanatical savages, through a mixture of luck, treachery, > and > the foulest inhumanity, to usurp their place for a few years. > -Richard Adams, "Shardik", 1974 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bill501 <@t> mindspring.com Mon Aug 4 19:01:19 2008 From: bill501 <@t> mindspring.com (Bill) Date: Mon Aug 4 19:01:32 2008 Subject: [Histonet] surveillance cameras in the lab In-Reply-To: <1CE1847DFEA0A647B1CCDE4108EA60A7F23E2E@LTA3VS011.ees.hhs.gov> References: <200808041246.1kq3cU5gg3Nl3oJ1@wanamaker.mail.atl.earthlink.net> <1CE1847DFEA0A647B1CCDE4108EA60A7F23E2A@LTA3VS011.ees.hhs.gov> <48973D57.7070901@umdnj.edu> <1CE1847DFEA0A647B1CCDE4108EA60A7F23E2E@LTA3VS011.ees.hhs.gov> Message-ID: At 1:39 PM -0400 8/4/08, Bartlett, Jeanine (CDC/CCID/NCZVED) wrote: >I agree they should have been notified and all that but my point is that >if I do not like the policies where I work I am free to go somewhere >else. > > >Jeanine Bartlett >Infectious Diseases Pathology Branch >(404) 639-3590 >jeanine.bartlett@cdc.hhs.gov Typical civil 'servant' attitude. How many are really free to up and leave. Smacks of the 'love it or leave it' crowd. BB From JMacDonald <@t> mtsac.edu Tue Aug 5 00:15:38 2008 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Aug 5 00:15:33 2008 Subject: [Histonet] HT schools In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B3701D3B0C3@giamail2.Gia.com> Message-ID: There are two routes to become eligible for the HT (ASCP) exam. One is successful completion of a NAACLS accredited program and the second is an AS degree (60 semester hours with 12 semester hours being science classes (biology and chemistry)) and one year full time acceptable experience in a histology laboratory. The experience is to include fixation, processing, microtomy and staining. NAACLS accredited programs can be either hospital based or college based. The hospital based programs have the advantage of training their students at their facility. College based programs have on campus labs, clinical rotations, or a combination of the two. Our program combines classroom experience and clinical work experience. Our student laboratory has 6 embedding centers and 24 microtomes. Our students learn to stain by hand, both H&E and specials. They also learn to coverslip by hand. Most of our clinical sites are automated so they get the automation experience in clinical rotation. On-line histotechnology programs were designed to help the person that has been working in the histology laboratory but cannot attend a formal program due to scheduling and time constraints. These are NAACLS accredited programs so they meet the standards of any NAACLS accredited program. The didactic (book learning) portion is taught on-line and the students are given practical assignments to complete in their laboratory. These are sent to the instructor of the on-line program. There are minimum education requirements for these programs. I think that it would be safe to say that most applicants going this route have lab experience already and the practical portion does not fall under the OJT definition as we knew it. I don't think that the loss of the practical portion of the HT exam has made any difference in the caliber of the applicants that are passing the exam. To meet either route 1 or 2 the applicant will need to turn in practical slides to an instructor. Having graded slides for the ASCP I can tell you that my students are held to the same or higher standard for their practical work. As for getting students into HT programs marketing is key. There are still many people that are not aware of Histotechnology as a viable and rewarding career. Our program will accept 24 students per year. The classes filled in the first few days of registration and we have a wait list of students trying to get into the program. We market to students at the college that are interested in science careers. We send a flyer to students enrolled in anatomy, physiology, chemistry, microbiology, and medical terminology. Many are undeclared majors that know they want something in science/healthcare but are not aware of all of the options. We also attend high school career fairs and career fairs for other colleges. We are invited to the other colleges because they do not have a histo program and want to expose their students to career choices. We also have a very healthy advisory committee that promote the program to employees in the labs that are presently not eligible for certification. As our graduates infiltrate the field there is also a good "word of mouth" recommendation from the grads. We also participate in a medical careers conference each year to promote the clinical laboratory sciences, with emphasis on histotechnology. Our college offers counseling classes for students to decide on a career path. I speak in these classes and invite the classes to the histo lab when the students are working. We also send career brochures to the guidance counselors in the high schools in the college area. Making students aware is vital to getting them to enroll. At the present time there is less than one program per state, not near enough to produce the histotechs needed. I would encourage anyone to start a program if the resources are available. Peggy Wenk has given workshops on how to set up a program. Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu From jdhisto <@t> yahoo.com Tue Aug 5 04:04:13 2008 From: jdhisto <@t> yahoo.com (JD) Date: Tue Aug 5 04:04:21 2008 Subject: [Histonet] HT schools Message-ID: Hello, I totally agree with Jennifer Mcdonalds comment. Yes it is all about marketing. People, students , most individuals do not know about Histology. They barely know what the word means, they usually think that its some kind of study of history (go figure). I have meet so many students repeatedly (biology, medical technology, chemistry majors) who love the idea of a career in histotechnology. Especially after finding out the many options there are besides being in a high volume lab cutting 200-300 blocks per day if not more. If people dont know...we dont grow. Till then.. Sincerely, JDhisto From tifei <@t> foxmail.com Tue Aug 5 04:21:24 2008 From: tifei <@t> foxmail.com (tf) Date: Tue Aug 5 04:21:54 2008 Subject: [Histonet] Gelatin embedding Message-ID: <200808051721189521540@foxmail.com> Hi All I am trying to cut some tiny and transected tissues. Given the cheese formation on cryostat, I would like to use microtome using gelatin or egg yolk as the embedding medium. Just wonder anyone has the detailed protocol? thanks a lot. 2008-08-05 tf From njoydobro <@t> aol.com Tue Aug 5 04:49:59 2008 From: njoydobro <@t> aol.com (njoydobro@aol.com) Date: Tue Aug 5 04:50:14 2008 Subject: [Histonet] protein block Message-ID: <8CAC4F7C2405C65-1280-153@WEBMAIL-STG-D01.sysops.aol.com> Good Morning everyone, ???? We are currently working up a new antibody and are trying it with and without protein block.? We are getting some unexpected results and I would like to see if anyone has seen similar outcomes.? We are getting less background without the protein block and the staining appears "crisper".? Anyone experienced this? Thanks, Gene From jqb7 <@t> cdc.gov Tue Aug 5 05:31:15 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Tue Aug 5 05:31:34 2008 Subject: [Histonet] surveillance cameras in the lab In-Reply-To: References: <200808041246.1kq3cU5gg3Nl3oJ1@wanamaker.mail.atl.earthlink.net> <1CE1847DFEA0A647B1CCDE4108EA60A7F23E2A@LTA3VS011.ees.hhs.gov> <48973D57.7070901@umdnj.edu> <1CE1847DFEA0A647B1CCDE4108EA60A7F23E2E@LTA3VS011.ees.hhs.gov> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70208BEF2@LTA3VS011.ees.hhs.gov> Nothing "typical" at all. I just understand that as an employer there may be a bigger picture than just me. I have worked in hospitals and also in private business so the civil "servant" comment is unnecessary and actually pretty demeaning. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bill Sent: Monday, August 04, 2008 8:01 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] surveillance cameras in the lab At 1:39 PM -0400 8/4/08, Bartlett, Jeanine (CDC/CCID/NCZVED) wrote: >I agree they should have been notified and all that but my point is >that if I do not like the policies where I work I am free to go >somewhere else. > > >Jeanine Bartlett >Infectious Diseases Pathology Branch >(404) 639-3590 >jeanine.bartlett@cdc.hhs.gov Typical civil 'servant' attitude. How many are really free to up and leave. Smacks of the 'love it or leave it' crowd. BB _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Tue Aug 5 05:52:01 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Aug 5 05:52:10 2008 Subject: [Histonet] surveillance cameras in the lab References: <200808041246.1kq3cU5gg3Nl3oJ1@wanamaker.mail.atl.earthlink.net><1CE1847DFEA0A647B1CCDE4108EA60A7F23E2A@LTA3VS011.ees.hhs.gov><48973D57.7070901@umdnj.edu><1CE1847DFEA0A647B1CCDE4108EA60A7F23E2E@LTA3VS011.ees.hhs.gov> <1CE1847DFEA0A647B1CCDE4108EA60A70208BEF2@LTA3VS011.ees.hhs.gov> Message-ID: <003e01c8f6e9$4ae58db0$e797b348@yourxhtr8hvc4p> I agree with Jeanine. I work as a civilian in a military hospital and I don't appreciate the "typical civil servant" comment either. And people tell me I'm ignorant. If you haven't walked in our shoes, then you don't know what you're talking about. Better to keep your mouth closed and have people think you're ignorant than open it and prove it. Joe Nocito BS, PA, HT(ASCP)QIHC San Antonio, TX ----- Original Message ----- From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" To: "Bill" ; Sent: Tuesday, August 05, 2008 5:31 AM Subject: RE: [Histonet] surveillance cameras in the lab Nothing "typical" at all. I just understand that as an employer there may be a bigger picture than just me. I have worked in hospitals and also in private business so the civil "servant" comment is unnecessary and actually pretty demeaning. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bill Sent: Monday, August 04, 2008 8:01 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] surveillance cameras in the lab At 1:39 PM -0400 8/4/08, Bartlett, Jeanine (CDC/CCID/NCZVED) wrote: >I agree they should have been notified and all that but my point is >that if I do not like the policies where I work I am free to go >somewhere else. > > >Jeanine Bartlett >Infectious Diseases Pathology Branch >(404) 639-3590 >jeanine.bartlett@cdc.hhs.gov Typical civil 'servant' attitude. How many are really free to up and leave. Smacks of the 'love it or leave it' crowd. BB _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmiller <@t> physlab.com Tue Aug 5 06:44:12 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Tue Aug 5 06:43:17 2008 Subject: [Histonet] HT schools In-Reply-To: References: <000d01c8f632$c218c4e0$3d02a8c0@plab.local> Message-ID: <000a01c8f6f0$94fb72f0$3d02a8c0@plab.local> I agree with you wholeheartedly. I am having the same issues with my training techs. What I am doing now to hone their practical skills is.. I participate in the Histo Qip program . I treat it as their practicum. This way I have an independent source looking at their work. Cheri Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: Patsy Ruegg [mailto:pruegg@ihctech.net] Sent: Monday, August 04, 2008 11:46 AM To: 'Cheri Miller'; rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; 'Amber McKenzie' Subject: RE: [Histonet] HT schools Remember that now there is no practical portion of the HT exam, so they are not being tested on hands on experiences anyway. The most difficult problem I have with training people on the job (and I have trained many) is that now they are not prepared to take the exam because they are examined all on theory. I have some really well trained people who can do the work really well, but they have a hard time taking the computer test which they pretty much have to memorize out of books. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Monday, August 04, 2008 7:05 AM To: rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; 'Amber McKenzie' Subject: RE: [Histonet] HT schools Someone has to teach them the "hands on" part of histology. I do not leave this to my staff. I teach / give them the skills they need to perform the practical part of their profession. As their supervisor I am very much involved in their training. I am sure I am not the only one.?? Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, August 01, 2008 2:42 PM To: histonet@lists.utsouthwestern.edu; Amber McKenzie Subject: Re: [Histonet] HT schools Wrong! The advantage of the "on line" or "distance learning" courses is that they provide the theory on line while you are working at a given laboratory doing your training (or even as part of your daily work) so there is no "actual training" to be done by the supervisor. At this moment NAACLS has 30 HT and 3 HTL accredited programs only, with an overall capacity of about 300-325 students, and this will not be enough to take care of? all the retiring histotechs. Costs is one of the reasons why the number of HTs schools is dwindling. Ren? J. ? --- On Fri, 8/1/08, Amber McKenzie wrote: From: Amber McKenzie Subject: [Histonet] HT schools To: histonet@lists.utsouthwestern.edu Date: Friday, August 1, 2008, 1:23 PM Where are all the HT accredited schools and why aren't there more out there? I've seen the online classes' people can take, but that requires them to be trained in a lab, as well, for the "hands on" part. So, actually the supervisor still has to train potential HT's "on the job" before they can sit for the board exam. Right? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. 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From mpence <@t> grhs.net Tue Aug 5 07:03:33 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Aug 5 07:03:37 2008 Subject: [Histonet] surveillance cameras in the lab In-Reply-To: <003e01c8f6e9$4ae58db0$e797b348@yourxhtr8hvc4p> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A38C2@IS-E2K3.grhs.net> ...and off with the gloves! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Tuesday, August 05, 2008 5:52 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Bill; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] surveillance cameras in the lab I agree with Jeanine. I work as a civilian in a military hospital and I don't appreciate the "typical civil servant" comment either. And people tell me I'm ignorant. If you haven't walked in our shoes, then you don't know what you're talking about. Better to keep your mouth closed and have people think you're ignorant than open it and prove it. Joe Nocito BS, PA, HT(ASCP)QIHC San Antonio, TX ----- Original Message ----- From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" To: "Bill" ; Sent: Tuesday, August 05, 2008 5:31 AM Subject: RE: [Histonet] surveillance cameras in the lab Nothing "typical" at all. I just understand that as an employer there may be a bigger picture than just me. I have worked in hospitals and also in private business so the civil "servant" comment is unnecessary and actually pretty demeaning. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bill Sent: Monday, August 04, 2008 8:01 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] surveillance cameras in the lab At 1:39 PM -0400 8/4/08, Bartlett, Jeanine (CDC/CCID/NCZVED) wrote: >I agree they should have been notified and all that but my point is >that if I do not like the policies where I work I am free to go >somewhere else. > > >Jeanine Bartlett >Infectious Diseases Pathology Branch >(404) 639-3590 >jeanine.bartlett@cdc.hhs.gov Typical civil 'servant' attitude. How many are really free to up and leave. Smacks of the 'love it or leave it' crowd. BB _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Tue Aug 5 07:14:31 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Tue Aug 5 07:14:50 2008 Subject: [Histonet] surveillance cameras in the lab In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A38C2@IS-E2K3.grhs.net> References: <003e01c8f6e9$4ae58db0$e797b348@yourxhtr8hvc4p> <661949901A768E4F9CC16D8AF8F2838C017A38C2@IS-E2K3.grhs.net> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70208BEFB@LTA3VS011.ees.hhs.gov> LOL! Okay, I guess I am done with this topic....at least until someone else offends me. :) Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Tuesday, August 05, 2008 8:04 AM To: Joe Nocito; Bartlett, Jeanine (CDC/CCID/NCZVED); Bill; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] surveillance cameras in the lab ...and off with the gloves! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Tuesday, August 05, 2008 5:52 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Bill; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] surveillance cameras in the lab I agree with Jeanine. I work as a civilian in a military hospital and I don't appreciate the "typical civil servant" comment either. And people tell me I'm ignorant. If you haven't walked in our shoes, then you don't know what you're talking about. Better to keep your mouth closed and have people think you're ignorant than open it and prove it. Joe Nocito BS, PA, HT(ASCP)QIHC San Antonio, TX ----- Original Message ----- From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" To: "Bill" ; Sent: Tuesday, August 05, 2008 5:31 AM Subject: RE: [Histonet] surveillance cameras in the lab Nothing "typical" at all. I just understand that as an employer there may be a bigger picture than just me. I have worked in hospitals and also in private business so the civil "servant" comment is unnecessary and actually pretty demeaning. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bill Sent: Monday, August 04, 2008 8:01 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] surveillance cameras in the lab At 1:39 PM -0400 8/4/08, Bartlett, Jeanine (CDC/CCID/NCZVED) wrote: >I agree they should have been notified and all that but my point is >that if I do not like the policies where I work I am free to go >somewhere else. > > >Jeanine Bartlett >Infectious Diseases Pathology Branch >(404) 639-3590 >jeanine.bartlett@cdc.hhs.gov Typical civil 'servant' attitude. How many are really free to up and leave. Smacks of the 'love it or leave it' crowd. BB _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Tue Aug 5 07:37:30 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Aug 5 07:37:11 2008 Subject: [Histonet] jcoleman IHCRG Membership Application Form Message-ID: Excuse me, I hit the wrong button again, this was to be for NSH which starts with histo just like the histonet, everyone at histonet disregard. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Monday, August 04, 2008 4:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] jcoleman IHCRG Membership Application Form Verify NSH membership please Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net www.ihcrg.org _____ From: webmaster@neo.agsci.colostate.edu [mailto:webmaster@neo.agsci.colostate.edu] Sent: Monday, August 04, 2008 2:45 PM To: patsy.ruegg@gmail.com Subject: IHCRG Membership Application Form _____ New_Application: Yes Last_Name: Coleman First_Name: John NSH_Member: Yes Employer: Sentara Healthcare Address: 600 Gresham Drive City: Norfolk State: VA Zip: 23507 Province: Country: USA Phone: (757) 335-2159 Ext: Fax: (757) 388- 3799 Email: jpcolema@sentara.com Surgical_Pathology: Yes Hematopathology: Yes Orthopedic: Veterinary: Immunology: Plastics: Research: GU Paraffin: Yes Frozen: Yes Cytospins: Smears_Touch_Preps: Yes Plastics_Sample: Sample_Other: PAP: APAAP: ABC_HRP: ABC_AP: SA_HRP: Yes SA_AP: Yes IF: Yes IHC_Other: ISH: Yes Gels: PCR: Mol_Other: Automated_IHC: Yes Company: Biogenex IHC_Qualification: Yes Date_Taken_Exam: 1997? Like_To_Take_Exam: No Expected_Date: Can_Provide_Tissues: Yes Tissues_Provided: Pneumocystis carinii, Hepatitis C, many others Need_Tissues: No Tissue_Needed: Remote Name: 163.230.7.6 HTTP User Agent: Mozilla/4.0 (compatible; MSIE 6.0; Windows NT 5.1; SV1; .NET CLR 1.1.4322) Date: 08/04/2008 Time: 02:44 PM Other_Tech Rapid Microwave processing _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Tue Aug 5 08:02:23 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue Aug 5 08:02:28 2008 Subject: [Histonet] p16 on Ventana Message-ID: <582736990808050602gf16ee97ief45480255ac5598@mail.gmail.com> Hi, There are a number of other p16 antibodies out there. This particular one is only sold by MTM. There are other clones they are probably RUO though, so you wouldn't be able to charge for it. How bad do the Dr's want it? Amos Brooks Message: 9 Date: Mon, 4 Aug 2008 09:59:33 -0500 From: "Sharon.Davis-Devine" Subject: [Histonet] p16 on Ventana To: Message-ID: <44780C571F28624DBB446DE55C4D733A021E0A51@EXCHANGEBE1.carle.com> Content-Type: text/plain; charset="us-ascii" Hey Histonetters! Is anyone out there in histoworld running p16 on the Ventana system? If so, can you provide us with your protocol and where you are ordering it from? We are aware that MTM is presently the only vendor for p16 but it is rather pricey and are wondering if there are any alternatives available out there. Once again thank you so much for your help. From moran.elish <@t> gmail.com Tue Aug 5 08:14:55 2008 From: moran.elish <@t> gmail.com (Moran Elishmereni) Date: Tue Aug 5 08:15:01 2008 Subject: [Histonet] can anyone detail the Tarpley method for staining mast cells and eosinophils? Message-ID: <4a722ef70808050614s42bccc30p5aea8688e89d7bfb@mail.gmail.com> Dear histonetters! I am in desperate need of the *detailed protocol* of the Tarpley method for staining mast cells and eosinophils. It is published as: "A new technique for the simultaneous specific staining of eosinophils and mast cells in paraffin tissue sectins." J Histotechnology, 7(3):141-142, 1984 J Tarpley et al However I cant get the hard copy... Can anyone help? Thanks! Moran -- Moran Elishmereni Department of Pharmacology and Experimental Therapeutics School of Pharmacy, Faculty of Medicine The Hebrew University of Jerusalem POB 12065 Jerusalem 91120, ISRAEL Tel: 972-2-675-8746 Fax: 972-2-675-8144 Email: moran.elish@gmail.com From amosbrooks <@t> gmail.com Tue Aug 5 08:23:42 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue Aug 5 08:23:52 2008 Subject: [Histonet] surveillance cameras in the lab Message-ID: <582736990808050623v3dac9e92xaec4f1a67e3a8b83@mail.gmail.com> Patsy, I have to disagree with you there. I understand the need for security, but there is a certain amount of trust lost when one makes the decision to monitor employees without even informing them as to why. (Weather or not they are legally bound to do so.) You can accomplish the same thing by letting employees know that there is a problem (or a potential problem) and having them beware and observant. As a last resort when they find the problem still presists using stromger methods becomes warranted. Once you give up this personal liberty and allow someone to watch over your shoulder all the time you loose the ability to make the right choices as there are no choices to make. I would rather live in a society of people that want to do the right thing than one of people that are forced to. At that point we might as well allow those that would do us harm to make all the decisions for us and abandon any form of personal freedom we may have. Ben Franklin published in Poor Richard's Almanac the famous quote *"Those who would give up essential liberty to purchase a little temporary safety, deserve neither liberty nor safety."* It is important to temper our fears with a sense of who we are lest we devolve into a military state. Thanks, Amos Brooks Message: 19 Date: Mon, 4 Aug 2008 10:46:26 -0600 From: "Patsy Ruegg" Subject: RE: [Histonet] surveillance cameras in the lab To: "'Cheri Miller'" , , Message-ID: Content-Type: text/plain; charset="us-ascii" Come on. In these times of terror concerns I am not sure I would work in a place where I did not feel secure and the use of these devices help in that matter. We built a brand new University of Colorado Health Sciences Center and there are cameras all over the place as well as lock down. If you do not have an access card you cannot get into the labs. This was a pain at first but with all the crazy's we have to worry about out there it now makes me feel better. I just read in the paper this morning about a researcher whose house was bombed by Peta types for doing animal research, and we have had all sorts of disturbances over the years with precious research animals being released, protests, etc. Patsy From Barry.R.Rittman <@t> uth.tmc.edu Tue Aug 5 08:25:53 2008 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Tue Aug 5 08:28:07 2008 Subject: [Histonet] surveillance cameras in the lab References: <582736990808050623v3dac9e92xaec4f1a67e3a8b83@mail.gmail.com> Message-ID: As one of my friends put it "No more picking your nose,sniffing under your armpits or taking care of any itches in private" Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Amos Brooks Sent: Tue 8/5/2008 8:23 AM To: pruegg@ihctech.net; histonet@lists.utsouthwestern.edu Subject: [Histonet] surveillance cameras in the lab Patsy, I have to disagree with you there. I understand the need for security, but there is a certain amount of trust lost when one makes the decision to monitor employees without even informing them as to why. (Weather or not they are legally bound to do so.) You can accomplish the same thing by letting employees know that there is a problem (or a potential problem) and having them beware and observant. As a last resort when they find the problem still presists using stromger methods becomes warranted. Once you give up this personal liberty and allow someone to watch over your shoulder all the time you loose the ability to make the right choices as there are no choices to make. I would rather live in a society of people that want to do the right thing than one of people that are forced to. At that point we might as well allow those that would do us harm to make all the decisions for us and abandon any form of personal freedom we may have. Ben Franklin published in Poor Richard's Almanac the famous quote *"Those who would give up essential liberty to purchase a little temporary safety, deserve neither liberty nor safety."* It is important to temper our fears with a sense of who we are lest we devolve into a military state. Thanks, Amos Brooks Message: 19 Date: Mon, 4 Aug 2008 10:46:26 -0600 From: "Patsy Ruegg" Subject: RE: [Histonet] surveillance cameras in the lab To: "'Cheri Miller'" , , Message-ID: Content-Type: text/plain; charset="us-ascii" Come on. In these times of terror concerns I am not sure I would work in a place where I did not feel secure and the use of these devices help in that matter. We built a brand new University of Colorado Health Sciences Center and there are cameras all over the place as well as lock down. If you do not have an access card you cannot get into the labs. This was a pain at first but with all the crazy's we have to worry about out there it now makes me feel better. I just read in the paper this morning about a researcher whose house was bombed by Peta types for doing animal research, and we have had all sorts of disturbances over the years with precious research animals being released, protests, etc. Patsy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Tue Aug 5 08:43:10 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue Aug 5 08:43:14 2008 Subject: [Histonet] Franklin Quote Message-ID: <582736990808050643t65bb6b59tb30521792f07dfc8@mail.gmail.com> OOPS I should have read the more recent messages before posting one of my own. Sorry about the redundancy of restating the quote. It is great that so many would mention it though. Gives one a bit of heart that the lessons of our forefathers (at least here in the USA) are still well ingrained. I'm sure the same is true overseas as liberty is not unique to this country Amos From rfields <@t> gidocs.net Tue Aug 5 08:45:33 2008 From: rfields <@t> gidocs.net (Rosa Fields) Date: Tue Aug 5 08:45:39 2008 Subject: [Histonet] HT exam In-Reply-To: <48883F0002000057000026B6@carrierpigeon.SummitHealth.local> References: <48883F0002000057000026B6@carrierpigeon.SummitHealth.local> Message-ID: <2F2611250DCD6549AA3D96CE8AF1F0180122078A@giexchange.gidocs.net> Yes, she is: Provided she has a letter from an appropriately board certified medical scientist stating she has experience in fixation, microtomy, processing, and staining.. I copied from the ASCP website: Route 2: Associate degree or at least 60 semester hours (90 quarter hours) of academic credit from a regionally accredited college/university, with a combination of 12 semester hours (18 quarter hours) of biology and chemistry, AND one year full time acceptable experience in histopathology in the U.S., Canada or a CAP/The Joint Commission (JCAHO)/AABB accredited laboratory within the last ten years under the supervision of a pathologist (certified by the American Board of Pathology in Anatomic Pathology), or an appropriately board certified medical scientist. Laboratory Experience To fulfill the experience requirement for the Histotechnician examination, you must have experience, within the last ten years, in the following areas: Fixation Microtomy Processing Staining -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rebecca Barnhart Sent: Thursday, July 24, 2008 7:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT exam I have a coworker that has an associates degree in Medical Office Administration. She has been trained for Histology and has worked in the field for two and a half years. Is she eligible for to sit for the ASCP HT exam? Thank you Becky _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lchausse <@t> nmh.org Tue Aug 5 09:25:49 2008 From: Lchausse <@t> nmh.org (Chaussey, Leslie) Date: Tue Aug 5 09:26:29 2008 Subject: [Histonet] HT exam In-Reply-To: <2F2611250DCD6549AA3D96CE8AF1F0180122078A@giexchange.gidocs.net> Message-ID: I believe she would also have to have the biology and chemistry credits specified though. That may not have been part of her associates degree. Leslie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rosa Fields Sent: Tuesday, August 05, 2008 8:46 AM To: Rebecca Barnhart; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT exam Yes, she is: Provided she has a letter from an appropriately board certified medical scientist stating she has experience in fixation, microtomy, processing, and staining.. I copied from the ASCP website: Route 2: Associate degree or at least 60 semester hours (90 quarter hours) of academic credit from a regionally accredited college/university, with a combination of 12 semester hours (18 quarter hours) of biology and chemistry, AND one year full time acceptable experience in histopathology in the U.S., Canada or a CAP/The Joint Commission (JCAHO)/AABB accredited laboratory within the last ten years under the supervision of a pathologist (certified by the American Board of Pathology in Anatomic Pathology), or an appropriately board certified medical scientist. Laboratory Experience To fulfill the experience requirement for the Histotechnician examination, you must have experience, within the last ten years, in the following areas: Fixation Microtomy Processing Staining -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rebecca Barnhart Sent: Thursday, July 24, 2008 7:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT exam I have a coworker that has an associates degree in Medical Office Administration. She has been trained for Histology and has worked in the field for two and a half years. Is she eligible for to sit for the ASCP HT exam? Thank you Becky _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- This message and any included attachments are intended only for the addressee. The information contained in this message is confidential and may constitute proprietary or non-public information under international, federal, or state laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail. From rfields <@t> gidocs.net Tue Aug 5 09:50:45 2008 From: rfields <@t> gidocs.net (Rosa Fields) Date: Tue Aug 5 09:50:52 2008 Subject: [Histonet] HT exam In-Reply-To: <346E5878979BA54FB4B0BFD6AD93B9B9B04131914B@EXCHMBC1.ad.ah.local> References: <48883F0002000057000026B6@carrierpigeon.SummitHealth.local> <2F2611250DCD6549AA3D96CE8AF1F0180122078A@giexchange.gidocs.net> <346E5878979BA54FB4B0BFD6AD93B9B9B04131914B@EXCHMBC1.ad.ah.local> Message-ID: <2F2611250DCD6549AA3D96CE8AF1F0180122079F@giexchange.gidocs.net> Good Point, I forget in my science world, that some people may have a degree that didn't include science classes!! -----Original Message----- From: Mahoney,Janice A [mailto:Janice.Mahoney@alegent.org] Sent: Tuesday, August 05, 2008 9:14 AM To: Rosa Fields; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] HT exam This is a key phrase: with a combination of 12 semester hours (18 quarter hours) of biology and chemistry, Jan -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rosa Fields Sent: Tuesday, August 05, 2008 8:46 AM To: Rebecca Barnhart; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT exam Yes, she is: Provided she has a letter from an appropriately board certified medical scientist stating she has experience in fixation, microtomy, processing, and staining.. I copied from the ASCP website: Route 2: Associate degree or at least 60 semester hours (90 quarter hours) of academic credit from a regionally accredited college/university, with a combination of 12 semester hours (18 quarter hours) of biology and chemistry, AND one year full time acceptable experience in histopathology in the U.S., Canada or a CAP/The Joint Commission (JCAHO)/AABB accredited laboratory within the last ten years under the supervision of a pathologist (certified by the American Board of Pathology in Anatomic Pathology), or an appropriately board certified medical scientist. Laboratory Experience To fulfill the experience requirement for the Histotechnician examination, you must have experience, within the last ten years, in the following areas: Fixation Microtomy Processing Staining -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rebecca Barnhart Sent: Thursday, July 24, 2008 7:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT exam I have a coworker that has an associates degree in Medical Office Administration. She has been trained for Histology and has worked in the field for two and a half years. Is she eligible for to sit for the ASCP HT exam? Thank you Becky _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. From bill501 <@t> mindspring.com Tue Aug 5 09:58:46 2008 From: bill501 <@t> mindspring.com (Bill) Date: Tue Aug 5 10:01:49 2008 Subject: [Histonet] surveillance cameras in the lab In-Reply-To: <003e01c8f6e9$4ae58db0$e797b348@yourxhtr8hvc4p> References: <200808041246.1kq3cU5gg3Nl3oJ1@wanamaker.mail.atl.earthlink.net><1CE1847DFEA0A647B1CCDE4108EA60A7F23E2A@LTA3V S011.ees.hhs.gov><48973D57.7070901@umdnj.edu><1CE1847DFEA0A647B1CCDE4108EA 60A7F23E2E@LTA3VS011.ees.hhs.gov> <1CE1847DFEA0A647B1CCDE4108EA60A70208BEF2@LTA3VS011.ees.hhs.gov> <003e01c8f6e9$4ae58db0$e797b348@yourxhtr8hvc4p> Message-ID: At 5:52 AM -0500 8/5/08, Joe Nocito wrote: >>>I agree with Jeanine. I work as a civilian in a military hospital and I don't appreciate the "typical civil servant" comment either. And people tell me I'm ignorant. If you haven't walked in our shoes, then you don't know what you're talking about. <<< It was meant to be demeaning. I have walked in your shoes, decided they were too little and confining, stunk and burned them, because I felt debased. So left. BB From mpence <@t> grhs.net Tue Aug 5 10:51:37 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Aug 5 10:51:42 2008 Subject: [Histonet] HT exam In-Reply-To: <2F2611250DCD6549AA3D96CE8AF1F0180122079F@giexchange.gidocs.net> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A38C4@IS-E2K3.grhs.net> But, it states " Associate degree or". It does not say Associates degree to include biology and chemistry. Everything from the "or" is another route within a route is it not? Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rosa Fields Sent: Tuesday, August 05, 2008 9:51 AM To: Mahoney,Janice A; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT exam Good Point, I forget in my science world, that some people may have a degree that didn't include science classes!! -----Original Message----- From: Mahoney,Janice A [mailto:Janice.Mahoney@alegent.org] Sent: Tuesday, August 05, 2008 9:14 AM To: Rosa Fields; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] HT exam This is a key phrase: with a combination of 12 semester hours (18 quarter hours) of biology and chemistry, Jan -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rosa Fields Sent: Tuesday, August 05, 2008 8:46 AM To: Rebecca Barnhart; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT exam Yes, she is: Provided she has a letter from an appropriately board certified medical scientist stating she has experience in fixation, microtomy, processing, and staining.. I copied from the ASCP website: Route 2: Associate degree or at least 60 semester hours (90 quarter hours) of academic credit from a regionally accredited college/university, with a combination of 12 semester hours (18 quarter hours) of biology and chemistry, AND one year full time acceptable experience in histopathology in the U.S., Canada or a CAP/The Joint Commission (JCAHO)/AABB accredited laboratory within the last ten years under the supervision of a pathologist (certified by the American Board of Pathology in Anatomic Pathology), or an appropriately board certified medical scientist. Laboratory Experience To fulfill the experience requirement for the Histotechnician examination, you must have experience, within the last ten years, in the following areas: Fixation Microtomy Processing Staining -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rebecca Barnhart Sent: Thursday, July 24, 2008 7:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT exam I have a coworker that has an associates degree in Medical Office Administration. She has been trained for Histology and has worked in the field for two and a half years. Is she eligible for to sit for the ASCP HT exam? Thank you Becky _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Tue Aug 5 10:53:43 2008 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Tue Aug 5 10:54:12 2008 Subject: [Histonet] surveillance cameras in the lab References: <661949901A768E4F9CC16D8AF8F2838C017A38C1@IS-E2K3.grhs.net> Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2751@fhosxchmb006.ADVENTISTCORP.NET> Count on it...not to mention the phones! Janet L. Bonner, HTL (ASCP) Pathology Laboratory Florida Hospital Winter Park janet.bonner@FLHOSP.org 407-646-7559 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Mike Pence Sent: Mon 8/4/2008 3:19 PM To: Markus F. Meyenhofer; Peter Carroll Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] surveillance cameras in the lab And maybe they are not just watching on their cameras, but also monitoring this entire thread on your computer. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Markus F. Meyenhofer Sent: Monday, August 04, 2008 12:55 PM To: Peter Carroll Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] surveillance cameras in the lab Well said (Ben's words) Thank you! ----- Original Message ----- From: "Peter Carroll" Cc: Sent: Monday, August 04, 2008 1:13 PM Subject: Re: [Histonet] surveillance cameras in the lab > > In these times of terror concerns I am not sure I would work in a > place where I did not feel secure and the use of these devices > > help in that matter. > > Wasn't it Ben Franklin who said "He who sacrifices freedom for > security > deserves neither"? > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From tifei <@t> foxmail.com Tue Aug 5 10:57:37 2008 From: tifei <@t> foxmail.com (tf) Date: Tue Aug 5 10:58:14 2008 Subject: [Histonet] Re: gelatin embedding References: Message-ID: <200808052357322136796@foxmail.com> SGkNCkkgdHJpZWQgdGhpcyBpbiBhIG11Y2ggc2ltcGxlIHdheS4NCg0KQWZ0ZXIgZml4YXRpb24g YW5kIDMwJSBzdWNyb3NlLCBwdXQgdGhlIGJyYWluIGludG8gMTAlIGdlbGF0aW4uDQpUaGVuIDM3 IGRlZ3JlZSBmb3IgMSBoLCB3aXRoIDQgJ0MgZm9yIDIwIG1pbi4NClRoZW4gcHV0IHRoZSBicmFp biBiYWNrIHRvIFBGQSBmb3IgMyBoLCBmb2xsb3dlZCB3aXRoIDQ4IGggMzAlIHN1Y3Jvc2UuDQpO b3cgeW91IGNhbiBnbyBtaWNyb3RvbWUuDQoNCg0KMjAwOC0wOC0wNSANCg0KDQoNCnRmIA0KDQoN Cg0Kt6K8/sjLo7ogU2FyYWggQm95ZCANCreiy83Ksbzko7ogMjAwOC0wOC0wNSAgMjM6MjU6NDEg DQrK1bz+yMujuiB0aWZlaUBmb3htYWlsLmNvbSANCrOty82juiANCtb3zOKjuiBnZWxhdGluIGVt YmVkZGluZyANCiANCkhpISAgSSBoYXZlIGFuIGVtYmVkZGluZyBwcm90b2NvbCwgYnV0IEkgd291 bGRuoa90IHNheSBpdKGvcyB0aGUgZ3JlYXRlc3QgZm9yIG91ciBhcHBsaWNhdGlvbiwgd2hpY2gg YXJlIHNjYWZmb2xkcy4gIEl0IG1heSB3b3JrIGZvciB5b3UgdGhvdWdoLiAgVGhlIG9ubHkgaXNz dWUgSSBoYXZlIHdpdGggaXQgaXMgdGhlIGhpc3RvIHN0YWluaW5nLiAgVGhlIGdlbGF0aW4gcGlj a3MgdXAgZXZlcnl0aGluZyEgIFdpbGwgeW91IHBsZWFzZSBsZXQgbWUga25vdyBpZiB5b3UgZ2V0 IGFub3RoZXIgZ29vZCBvbmUgZnJvbSBzb21lb25lIGVsc2U/ICBJIHdvdWxkIGJlIGludGVyZXN0 ZWQgaW4gdHJ5aW5nIGFub3RoZXIgb25lLiAgVGhpcyBwcm90b2NvbCBpcyB3cml0dGVuIGZvciB1 c2Ugd2l0aCBzY2FmZm9sZHMgYnV0IHlvdSBjYW4gbW9kaWZ5IGl0IGZvciB5b3VyIHVzZS4NClNh cmFoDQo= From rfields <@t> gidocs.net Tue Aug 5 11:06:43 2008 From: rfields <@t> gidocs.net (Rosa Fields) Date: Tue Aug 5 11:06:47 2008 Subject: [Histonet] HT exam In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A38C4@IS-E2K3.grhs.net> References: <2F2611250DCD6549AA3D96CE8AF1F0180122079F@giexchange.gidocs.net> <661949901A768E4F9CC16D8AF8F2838C017A38C4@IS-E2K3.grhs.net> Message-ID: <2F2611250DCD6549AA3D96CE8AF1F018012207C2@giexchange.gidocs.net> It is probably debatable, the ASCP accepted my letter from the lab director of the Veterinary Diagnostic Lab I was employed at, but a co-worker who wished to take the exam for the specialist certification in virology was denied, the reason being that the veterinary pathologist lab director was unqualified, mind you the letter was from the same person, go figure! How could the same individual be qualified in one case and not another? Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited.? -----Original Message----- From: Mike Pence [mailto:mpence@grhs.net] Sent: Tuesday, August 05, 2008 10:52 AM To: Rosa Fields; Mahoney,Janice A; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT exam But, it states " Associate degree or". It does not say Associates degree to include biology and chemistry. Everything from the "or" is another route within a route is it not? Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rosa Fields Sent: Tuesday, August 05, 2008 9:51 AM To: Mahoney,Janice A; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT exam Good Point, I forget in my science world, that some people may have a degree that didn't include science classes!! -----Original Message----- From: Mahoney,Janice A [mailto:Janice.Mahoney@alegent.org] Sent: Tuesday, August 05, 2008 9:14 AM To: Rosa Fields; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] HT exam This is a key phrase: with a combination of 12 semester hours (18 quarter hours) of biology and chemistry, Jan -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rosa Fields Sent: Tuesday, August 05, 2008 8:46 AM To: Rebecca Barnhart; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT exam Yes, she is: Provided she has a letter from an appropriately board certified medical scientist stating she has experience in fixation, microtomy, processing, and staining.. I copied from the ASCP website: Route 2: Associate degree or at least 60 semester hours (90 quarter hours) of academic credit from a regionally accredited college/university, with a combination of 12 semester hours (18 quarter hours) of biology and chemistry, AND one year full time acceptable experience in histopathology in the U.S., Canada or a CAP/The Joint Commission (JCAHO)/AABB accredited laboratory within the last ten years under the supervision of a pathologist (certified by the American Board of Pathology in Anatomic Pathology), or an appropriately board certified medical scientist. Laboratory Experience To fulfill the experience requirement for the Histotechnician examination, you must have experience, within the last ten years, in the following areas: Fixation Microtomy Processing Staining -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rebecca Barnhart Sent: Thursday, July 24, 2008 7:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT exam I have a coworker that has an associates degree in Medical Office Administration. She has been trained for Histology and has worked in the field for two and a half years. Is she eligible for to sit for the ASCP HT exam? Thank you Becky _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Tue Aug 5 11:21:30 2008 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Aug 5 11:21:27 2008 Subject: [Histonet] HT exam In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A38C4@IS-E2K3.grhs.net> Message-ID: AS degree or 60 semester hours of academic credit, but the remainder of the statement is required for route 2. Not everyone that completes at least 60 semester hours of college credit applies for the AS degree or they may not meet the course requirements for an AS degree. The ASCP is willing to accept the credit as long as it includes the necessary science courses. "Mike Pence" Sent by: histonet-bounces@lists.utsouthwestern.edu 08/05/2008 08:54 AM To "Rosa Fields" , "Mahoney,Janice A" , cc Subject RE: [Histonet] HT exam But, it states " Associate degree or". It does not say Associates degree to include biology and chemistry. Everything from the "or" is another route within a route is it not? Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rosa Fields Sent: Tuesday, August 05, 2008 9:51 AM To: Mahoney,Janice A; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT exam Good Point, I forget in my science world, that some people may have a degree that didn't include science classes!! -----Original Message----- From: Mahoney,Janice A [mailto:Janice.Mahoney@alegent.org] Sent: Tuesday, August 05, 2008 9:14 AM To: Rosa Fields; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] HT exam This is a key phrase: with a combination of 12 semester hours (18 quarter hours) of biology and chemistry, Jan -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rosa Fields Sent: Tuesday, August 05, 2008 8:46 AM To: Rebecca Barnhart; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] HT exam Yes, she is: Provided she has a letter from an appropriately board certified medical scientist stating she has experience in fixation, microtomy, processing, and staining.. I copied from the ASCP website: Route 2: Associate degree or at least 60 semester hours (90 quarter hours) of academic credit from a regionally accredited college/university, with a combination of 12 semester hours (18 quarter hours) of biology and chemistry, AND one year full time acceptable experience in histopathology in the U.S., Canada or a CAP/The Joint Commission (JCAHO)/AABB accredited laboratory within the last ten years under the supervision of a pathologist (certified by the American Board of Pathology in Anatomic Pathology), or an appropriately board certified medical scientist. Laboratory Experience To fulfill the experience requirement for the Histotechnician examination, you must have experience, within the last ten years, in the following areas: Fixation Microtomy Processing Staining -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rebecca Barnhart Sent: Thursday, July 24, 2008 7:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HT exam I have a coworker that has an associates degree in Medical Office Administration. She has been trained for Histology and has worked in the field for two and a half years. Is she eligible for to sit for the ASCP HT exam? Thank you Becky _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tjasper <@t> copc.net Tue Aug 5 11:26:01 2008 From: tjasper <@t> copc.net (Thomas Jasper) Date: Tue Aug 5 11:26:06 2008 Subject: [Histonet] PSLIM Message-ID: <90354A475B420441B2A0396E5008D4965E2136@copc-sbs.COPC.local> Am joining the chorus here on feedback re: PSLIM. Any users out there? Haven't seen anyone respond yet. I think we're all very interested in opinions good, bad or indifferent. Thanks, Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, OR 97701 541/693-2677 tjasper@copc.net From kalschev <@t> svm.vetmed.wisc.edu Tue Aug 5 11:47:09 2008 From: kalschev <@t> svm.vetmed.wisc.edu (Vicki Kalscheur) Date: Tue Aug 5 11:47:57 2008 Subject: [Histonet] Hard Tissue Committee Meeting at NSH 2008` Message-ID: <028701c8f71a$e6d9c160$c5d76880@vetmed.wisc.edu> Dear Members and Interested Attendees: The month of August will pass quickly. This is a reminder to plan ahead and join us on Saturday, September 13th, 2008 from 12:00 pm to 12:45 pm in Pittsburgh, PA as we convene at NSH's 34th Annual Symposium. The David L. Lawrence Convention Center meeting room will be posted at the Hard Tissue Display Table. I look forward to seeing many of you. Best Regards, Vicki Kalscheur, Committee Chair From mike <@t> pathview.com Tue Aug 5 11:48:56 2008 From: mike <@t> pathview.com (Michael Mihalik) Date: Tue Aug 5 11:49:54 2008 Subject: [Histonet] PSLIM In-Reply-To: <90354A475B420441B2A0396E5008D4965E2136@copc-sbs.COPC.local> References: <90354A475B420441B2A0396E5008D4965E2136@copc-sbs.COPC.local> Message-ID: <002401c8f71b$3ebb7770$bc326650$@com> Yes, please. Any feedback would be great. I"ve done some preliminary research for use in our software product. Currently, we use: Zebra 3844 1. ~500 cost 2. uses labels and therefore there is a reoccurring cost. 3. labels must be manually applied PSLIM product 1. ~5000 cost 2. no labels, but uses frosted slides, I believe -- there's a reoccurring cost there. 3. no labels to apply. They both use thermal ribbons, print 2 D barcodes, and print 'fast'. The pslim product has an interesting feature that some labs will find critical. You can have it determine what to put on slide. The zebra product only prints what your LIS vendor tells it to print. I would appreciate any feedback. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Tuesday, August 05, 2008 12:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PSLIM Am joining the chorus here on feedback re: PSLIM. Any users out there? Haven't seen anyone respond yet. I think we're all very interested in opinions good, bad or indifferent. Thanks, Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, OR 97701 541/693-2677 tjasper@copc.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BrealK <@t> alexian.net Tue Aug 5 12:15:53 2008 From: BrealK <@t> alexian.net (Kari Breal) Date: Tue Aug 5 12:16:08 2008 Subject: [Histonet] Cost per test Message-ID: <20080805T121553Z_439500110000@alexian.net> Does anyone have a cost per slide/block formula for histology work they would be willing to share? Thanks, Kari Breal Histology Supervisor Alexian Brothers Medical Center 847-437-5500 ext. 5155 Fax 847-981-2023 brealk@alexian.net CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From marilynng <@t> earthlink.net Tue Aug 5 12:19:50 2008 From: marilynng <@t> earthlink.net (Marilyn Gamble) Date: Tue Aug 5 12:19:57 2008 Subject: [Histonet] HT Exam clarification Message-ID: <380-22008825171950968@earthlink.net> The certification criteria is listed below. Under Route 2, please note the last sentence. If the applicant is under the supervision of a BOR, board certified technician/technologist, the supervisor can sign the attestation form to validate the experience. Explanation: In the case of Histopathology, the (board certified) supervisor is recognized as an "appropriately board certified medical scientist." Criteria: Histotechnician, HT(ASCP) To be eligible for this examination category, an applicant must satisfy the requirements of at least one of the following routes: Route 1: Successful completion of a NAACLS accredited Histotechnician program within the last 5 years prior to the date of application for examination; or Route 2: Associate degree or at least 60 semester hours (90 quarter hours) of academic credit from a regionally accredited college/university, with a combination of 12 semester hours (18 quarter hours) of biology and chemistry, AND one year full time acceptable experience in histopathology in the U.S., Canada or a CAP/The Joint Commission (JCAHO)/AABB accredited laboratory within the last ten years under the supervision of a pathologist (certified by the American Board of Pathology in Anatomic Pathology), or an appropriately board certified medical scientist. Laboratory Experience To fulfill the experience requirement for the Histotechnician examination, you must have experience, within the last ten years, in the following areas: Fixation Microtomy Processing Staining Marilyn Gamble marilynng@earthlink.net From Valerie.Hannen <@t> parrishmed.com Tue Aug 5 12:29:45 2008 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Tue Aug 5 12:30:25 2008 Subject: [Histonet] knife sharpener lapping plate In-Reply-To: <1B73766A27A1554CB2729B6432E81301B2F9CA@KALEXMB04.KaleidaHealth.org> Message-ID: <5680DA93771F0C48954CC8D38425E72401AB34DF@ISMAIL.parrishmed.local> We replace oour lapping plates when they get so thin that they can not be properly "resurfaced" or honed. If they slip off of each other when they are being honed...they are too thin. Valerie Hannen, MLT(ASCP),HTL,SU(FL) Parrish Medical Center Titusville,Florida -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DiCarlo, Margaret Sent: Friday, August 01, 2008 8:24 AM To: histonet@pathology.swmed.edu Subject: [Histonet] knife sharpener lapping plate Histonetters, For those of you who still use the Shandon Autosharp 5 for knife sharpening like me, how do you know when you need to replace the lapping plates? Thanks. Peggy DiCarlo (HT) Ortho Bone Lab Buffalo General Hospital 716-859-1293 2007 Best Places to Work Finalist Visit our careers page at www.kaleidahealth.org/careers CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you From TMcNemar <@t> lmhealth.org Tue Aug 5 12:32:49 2008 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Tue Aug 5 12:32:44 2008 Subject: [Histonet] Quick dry coverslipping media Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F5A6@lmhsmail.lmhealth.org> We are being buried in slides. We are currently using Cytoseal 60 from Richard Alan as a coverslipping media but it takes a long time for it to dry. Does anyone knkow of a coverslipping media that would allow us to file our slides within a couple of days without them sticking together? Thanks! Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org From Elizabeth.A.Allen <@t> Hitchcock.ORG Tue Aug 5 12:38:37 2008 From: Elizabeth.A.Allen <@t> Hitchcock.ORG (Elizabeth A. Allen) Date: Tue Aug 5 12:39:00 2008 Subject: [Histonet] question regarding crystal violet stain...... Message-ID: <42188051@mailbox4.Hitchcock.ORG> I am trying to find a procedure for staining fresh muscle tissue for crystal violet to demonstrate amyloid. Does anyone have a protocol they could send me? Thank you. Beth From trathborne <@t> somerset-healthcare.com Tue Aug 5 12:41:56 2008 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Aug 5 12:42:05 2008 Subject: [Histonet] Quick dry coverslipping media In-Reply-To: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F5A6@lmhsmail.lmhealth.org> Message-ID: We use Richard-Allan mounting medium and file slides the next day. Catalog #4111 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Tom McNemar Sent: Tuesday, August 05, 2008 1:33 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Quick dry coverslipping media We are being buried in slides. We are currently using Cytoseal 60 from Richard Alan as a coverslipping media but it takes a long time for it to dry. Does anyone knkow of a coverslipping media that would allow us to file our slides within a couple of days without them sticking together? Thanks! Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. From zodiac29 <@t> comcast.net Tue Aug 5 12:48:05 2008 From: zodiac29 <@t> comcast.net (zodiac29@comcast.net) Date: Tue Aug 5 12:48:13 2008 Subject: [Histonet] Alcian blue/PAS Message-ID: <080520081748.21750.48989255000CB90F000054F62216554886C7CD0C0E070B0196@comcast.net> Hello All, I wanted to know what variation of the alcian blue/PAS stain that you like best. We are going to start doing this stain instead of the alcian blue that we do now. The texts that I use (Carson, Bancroft, AFIP) all have diffrent variations. I just wanted some imput on what is best. Thank you Jenny From lblazek <@t> digestivespecialists.com Tue Aug 5 12:57:58 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Aug 5 12:50:11 2008 Subject: [Histonet] RE: Quick dry coverslipping media In-Reply-To: References: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F5A6@lmhsmail.lmhealth.org> Message-ID: <5A2BD13465E061429D6455C8D6B40E390B77E35D@IBMB7Exchange.digestivespecialists.com> I use that as well and have no problem with next day filing. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, August 05, 2008 1:42 PM To: Tom McNemar; histonet@pathology.swmed.edu Subject: RE: [Histonet] Quick dry coverslipping media We use Richard-Allan mounting medium and file slides the next day. Catalog #4111 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Tom McNemar Sent: Tuesday, August 05, 2008 1:33 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Quick dry coverslipping media We are being buried in slides. We are currently using Cytoseal 60 from Richard Alan as a coverslipping media but it takes a long time for it to dry. Does anyone knkow of a coverslipping media that would allow us to file our slides within a couple of days without them sticking together? Thanks! Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. From JWeems <@t> sjha.org Tue Aug 5 12:52:25 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Aug 5 12:52:30 2008 Subject: [Histonet] Quick dry coverslipping media In-Reply-To: References: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F5A6@lmhsmail.lmhealth.org> Message-ID: <982A0A9461F9BF438C7B19A6E425A3834AFB0D@ITSSSXM01V6.one.ads.che.org> Does it work well with automatic coverslippers? Thanks, j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, August 05, 2008 1:42 PM To: Tom McNemar; histonet@pathology.swmed.edu Subject: RE: [Histonet] Quick dry coverslipping media We use Richard-Allan mounting medium and file slides the next day. Catalog #4111 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Tom McNemar Sent: Tuesday, August 05, 2008 1:33 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Quick dry coverslipping media We are being buried in slides. We are currently using Cytoseal 60 from Richard Alan as a coverslipping media but it takes a long time for it to dry. Does anyone knkow of a coverslipping media that would allow us to file our slides within a couple of days without them sticking together? Thanks! Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From lblazek <@t> digestivespecialists.com Tue Aug 5 13:10:17 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Aug 5 13:02:23 2008 Subject: [Histonet] Quick dry coverslipping media In-Reply-To: <982A0A9461F9BF438C7B19A6E425A3834AFB0D@ITSSSXM01V6.one.ads.che.org> References: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F5A6@lmhsmail.lmhealth.org> <982A0A9461F9BF438C7B19A6E425A3834AFB0D@ITSSSXM01V6.one.ads.che.org> Message-ID: <5A2BD13465E061429D6455C8D6B40E390B77E35F@IBMB7Exchange.digestivespecialists.com> I use it with my coverslipper. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Tuesday, August 05, 2008 1:52 PM To: Rathborne, Toni; Tom McNemar; histonet@pathology.swmed.edu Subject: RE: [Histonet] Quick dry coverslipping media Does it work well with automatic coverslippers? Thanks, j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, August 05, 2008 1:42 PM To: Tom McNemar; histonet@pathology.swmed.edu Subject: RE: [Histonet] Quick dry coverslipping media We use Richard-Allan mounting medium and file slides the next day. Catalog #4111 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Tom McNemar Sent: Tuesday, August 05, 2008 1:33 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Quick dry coverslipping media We are being buried in slides. We are currently using Cytoseal 60 from Richard Alan as a coverslipping media but it takes a long time for it to dry. Does anyone knkow of a coverslipping media that would allow us to file our slides within a couple of days without them sticking together? Thanks! Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From trathborne <@t> somerset-healthcare.com Tue Aug 5 13:03:09 2008 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Aug 5 13:03:15 2008 Subject: [Histonet] Quick dry coverslipping media In-Reply-To: <982A0A9461F9BF438C7B19A6E425A3834AFB0D@ITSSSXM01V6.one.ads.che.org> Message-ID: Yes. We use it on our Leica CV5030. -----Original Message----- From: Weems, Joyce [mailto:JWeems@sjha.org] Sent: Tuesday, August 05, 2008 1:52 PM To: Rathborne, Toni; Tom McNemar; histonet@pathology.swmed.edu Subject: RE: [Histonet] Quick dry coverslipping media Does it work well with automatic coverslippers? Thanks, j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, August 05, 2008 1:42 PM To: Tom McNemar; histonet@pathology.swmed.edu Subject: RE: [Histonet] Quick dry coverslipping media We use Richard-Allan mounting medium and file slides the next day. Catalog #4111 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Tom McNemar Sent: Tuesday, August 05, 2008 1:33 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Quick dry coverslipping media We are being buried in slides. We are currently using Cytoseal 60 from Richard Alan as a coverslipping media but it takes a long time for it to dry. Does anyone knkow of a coverslipping media that would allow us to file our slides within a couple of days without them sticking together? Thanks! Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. From pumla.pamla <@t> gmail.com Tue Aug 5 13:50:03 2008 From: pumla.pamla <@t> gmail.com (Pumla Pamla-Gutter) Date: Tue Aug 5 13:50:08 2008 Subject: [Histonet] Re: Histonet Digest, Vol 57, Issue 9 In-Reply-To: <4898879d.3d742c0a.68ad.ffffa86dSMTPIN_ADDED@mx.google.com> References: <4898879d.3d742c0a.68ad.ffffa86dSMTPIN_ADDED@mx.google.com> Message-ID: Dear All, Any feedback appreciated. I've always used Cresyl Violet Echt, and now I've just switched to Cresyl Violet acetate. Does anyone know if I could treat these two the same way? I've been using 5g of the cresyl in 1000ml of dH2O. Does anyone work with this Nissl body stain and if so what protocol do you use? Thank you. Pumla From rjbuesa <@t> yahoo.com Tue Aug 5 15:21:53 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Aug 5 15:21:56 2008 Subject: [Histonet] Cost per test In-Reply-To: <20080805T121553Z_439500110000@alexian.net> Message-ID: <286694.63074.qm@web65704.mail.ac4.yahoo.com> Under separate cover I am sending you an article I wrote on the subject. Ren? J. --- On Tue, 8/5/08, Kari Breal wrote: From: Kari Breal Subject: [Histonet] Cost per test To: histonet@lists.utsouthwestern.edu Date: Tuesday, August 5, 2008, 1:15 PM Does anyone have a cost per slide/block formula for histology work they would be willing to share? Thanks, Kari Breal Histology Supervisor Alexian Brothers Medical Center 847-437-5500 ext. 5155 Fax 847-981-2023 brealk@alexian.net CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> pathview.com Tue Aug 5 16:41:43 2008 From: mike <@t> pathview.com (Michael Mihalik) Date: Tue Aug 5 16:42:34 2008 Subject: [Histonet] computer system volunteers? In-Reply-To: References: <4898879d.3d742c0a.68ad.ffffa86dSMTPIN_ADDED@mx.google.com> Message-ID: <00bb01c8f744$1f138970$5d3a9c50$@com> Good evening, I'm looking for some volunteers to give us some input on billing features that you absolutely love or would love to have in your system. Basically, we're enhancing the billing aspect of our AP system and we'd like to get as many opinions as possible. Of course, we've already started with our current clients, but we'd like to expand our horizons. So, if you don't mind sharing some of the best parts, worst parts, or even fantasies, I'd appreciate it. To start with, our overall objective is to make billing as automatic as possible. Why we involve our technologists or pathologists in billing has always been beyond me. Your jobs should be to focus on performing the task as hand, and to me, at least, that's not about billing. ...though I know we have to bill to stay in existence. I appreciate whatever you can give me. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pumla Pamla-Gutter Sent: Tuesday, August 05, 2008 2:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 57, Issue 9 Dear All, Any feedback appreciated. I've always used Cresyl Violet Echt, and now I've just switched to Cresyl Violet acetate. Does anyone know if I could treat these two the same way? I've been using 5g of the cresyl in 1000ml of dH2O. Does anyone work with this Nissl body stain and if so what protocol do you use? Thank you. Pumla _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BERGERJL <@t> aol.com Tue Aug 5 19:07:50 2008 From: BERGERJL <@t> aol.com (BERGERJL@aol.com) Date: Tue Aug 5 19:08:00 2008 Subject: [Histonet] Horse bones Message-ID: We are trying to section pieces of horse femurs, fetlock and carpal joints. They have been decalcified with either HCl decal or formic acid decal. Before routine processing the bones appear to be decalcified, but at sectioning they are very hard and brittle (even after surface decal) and chip out of the paraffin block. Any suggestions would be helpful. A pathologist suggested a soap soaking solution but could not remember the name of the soap. Help and thank you, R. Berger, HT **************Looking for a car that's sporty, fun and fits in your budget? Read reviews on AOL Autos. (http://autos.aol.com/cars-BMW-128-2008/expert-review?ncid=aolaut00050000000017 ) From kwuny <@t> email.cs.nsw.gov.au Tue Aug 5 21:14:36 2008 From: kwuny <@t> email.cs.nsw.gov.au (Young Kwun) Date: Tue Aug 5 21:14:56 2008 Subject: [Histonet] Factor XIIIa antibody Message-ID: <200808061214847.SM00940@csls2816> Hello All, We've been using Factor XIII, A-subunit polyclonal antibody from Calbiochem (Cat.No.233498) on skin conditions successfully so far. However this antibody was discontinued few years ago and they are offering Factor XIII, S-subunit only. Although I haven't tried FXIII S-unit immunostaining on human skin, I tried two other monoclonal Factor XIIIa but the stains were not as good as our current one. I tried Neomaker and Novocastra's. Can anyone suggest the source of good FXIIIa please? Thank you. Young Kwun Senior Hospital Scientist Dept. of Anatomical Pathology Concord Hospital Concord NSW 2139 Australia Tel)61-2-9767-6075 Fax)61-2-9767-8427 kwuny@email.cs.nsw.gov.au From AnthonyH <@t> chw.edu.au Tue Aug 5 23:05:56 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Aug 5 23:06:03 2008 Subject: [Histonet] surveillance cameras in the lab In-Reply-To: Message-ID: If you like, I can come and clean the lenses, at a modest price (say 10c/hr) plus travelling. My microscope lenses always look worse after I clean them, so I should have the same success with your camera lenses. By the way, I live in Sydney, Australia!! But seriously, what a dumb move, to install cameras without consultation!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bill Sent: Tuesday, 5 August 2008 12:02 AM To: Pathrm35@comcast.net; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] surveillance cameras in the lab At 10:21 AM +0000 8/4/08, Pathrm35@comcast.net wrote: >>>I was wondering how many techs out there have cameras in their labs, >>>either for security or to monitor employees. I went to work Sunday >>>night and noticed that 4 cameras were installed in the lab over the >>>weekend, with more to come.<<< I would use immersion oil on the camera lenses. -- ______________ Bill Blank, MD Heartland Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Tue Aug 5 23:31:10 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Aug 5 23:31:21 2008 Subject: [Histonet] Factor XIIIa antibody In-Reply-To: <200808061214847.SM00940@csls2816> Message-ID: Hi Young, How is my old lab going? Try Biogenix Cat No: MU337-UP Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Young Kwun Sent: Wednesday, 6 August 2008 12:15 PM To: Histonet Subject: [Histonet] Factor XIIIa antibody Hello All, We've been using Factor XIII, A-subunit polyclonal antibody from Calbiochem (Cat.No.233498) on skin conditions successfully so far. However this antibody was discontinued few years ago and they are offering Factor XIII, S-subunit only. Although I haven't tried FXIII S-unit immunostaining on human skin, I tried two other monoclonal Factor XIIIa but the stains were not as good as our current one. I tried Neomaker and Novocastra's. Can anyone suggest the source of good FXIIIa please? Thank you. Young Kwun Senior Hospital Scientist Dept. of Anatomical Pathology Concord Hospital Concord NSW 2139 Australia Tel)61-2-9767-6075 Fax)61-2-9767-8427 kwuny@email.cs.nsw.gov.au _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From stevenhacker <@t> verizon.net Tue Aug 5 23:43:32 2008 From: stevenhacker <@t> verizon.net (Steven Hacker) Date: Tue Aug 5 23:43:40 2008 Subject: [Histonet] Factor XIIIa antibody Message-ID: <18418115.7124031217997812488.JavaMail.root@vms068.mailsrvcs.net> Please take a look at BioCare Medical's Factor XIIIa Ab... ===================== From: Tony Henwood Date: 2008/08/06 Wed AM 12:31:10 EDT To: Young Kwun , Histonet Subject: RE: [Histonet] Factor XIIIa antibody Hi Young, How is my old lab going? Try Biogenix Cat No: MU337-UP Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Young Kwun Sent: Wednesday, 6 August 2008 12:15 PM To: Histonet Subject: [Histonet] Factor XIIIa antibody Hello All, We've been using Factor XIII, A-subunit polyclonal antibody from Calbiochem (Cat.No.233498) on skin conditions successfully so far. However this antibody was discontinued few years ago and they are offering Factor XIII, S-subunit only. Although I haven't tried FXIII S-unit immunostaining on human skin, I tried two other monoclonal Factor XIIIa but the stains were not as good as our current one. I tried Neomaker and Novocastra's. Can anyone suggest the source of good FXIIIa please? Thank you. Young Kwun Senior Hospital Scientist Dept. of Anatomical Pathology Concord Hospital Concord NSW 2139 Australia Tel)61-2-9767-6075 Fax)61-2-9767-8427 kwuny@email.cs.nsw.gov.au _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Wed Aug 6 02:58:41 2008 From: louise.renton <@t> gmail.com (louise renton) Date: Wed Aug 6 02:58:46 2008 Subject: [Histonet] Horse bones In-Reply-To: References: Message-ID: Some things to think about.... Rinse the tissue well with running tap water after decal and before processing - this seems to make a difference (depends on the size of the bone - 2hrs to overnight) EMbed the bones so that the long axis is either at right angles or 45 degrees to the blade so that the edge of the knife doesn't have to cut through a narrow band of cortical bone along its entire length - this causes "skipping"(If this is unclear I can send you an attachment with a diagramme) Once you have faced the blocks put the face down on ice in a -20 deg freezer overnight (chest type freezer). then remove the one ata time for sectioning. I have only had limited sucess with soaking the faced blocks in fabric softener undiluted. Hopes this helps best regards On 8/6/08, BERGERJL@aol.com wrote: > > We are trying to section pieces of horse femurs, fetlock and > carpal joints. > They have been decalcified with either HCl decal or formic acid decal. > Before routine processing the bones appear to be decalcified, but at > sectioning > they are very hard and brittle (even after surface decal) and chip out of > the > paraffin block. > Any suggestions would be helpful. A pathologist suggested a soap soaking > solution but could not remember the name of the soap. > > Help and thank you, > > R. Berger, HT > > > > > **************Looking for a car that's sporty, fun and fits in your budget? > Read reviews on AOL Autos. > ( > http://autos.aol.com/cars-BMW-128-2008/expert-review?ncid=aolaut00050000000017) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From pieronelva01 <@t> bigpond.com Wed Aug 6 03:42:48 2008 From: pieronelva01 <@t> bigpond.com (Piero Nelva) Date: Wed Aug 6 03:42:51 2008 Subject: [Histonet] Horse bones References: Message-ID: <006801c8f7a0$67fd9840$d175be7c@pentium4> For tough or brittle, but not necessarily calcified tissue, I find that soaking the block on a water soaked tissue for 10-15 minutes effective for softening. It can sometimes take all day to get a section, as trimming sometimes has to be done 4-5 microns at a time!! Piero Nelva Monash Medical Centre Victoria Australia ----- Original Message ----- From: "louise renton" To: ; Sent: Wednesday, August 06, 2008 5:58 PM Subject: Re: [Histonet] Horse bones > Some things to think about.... > > Rinse the tissue well with running tap water after decal and before > processing - this seems to make a difference (depends on the size of the > bone - 2hrs to overnight) > > EMbed the bones so that the long axis is either at right angles or 45 > degrees to the blade so that the edge of the knife doesn't have to cut > through a narrow band of cortical bone along its entire length - this > causes > "skipping"(If this is unclear I can send you an attachment with a > diagramme) > > Once you have faced the blocks put the face down on ice in a -20 deg > freezer > overnight (chest type freezer). then remove the one ata time for > sectioning. > > I have only had limited sucess with soaking the faced blocks in fabric > softener undiluted. > > Hopes this helps > > best regards > > > On 8/6/08, BERGERJL@aol.com wrote: >> >> We are trying to section pieces of horse femurs, fetlock and >> carpal joints. >> They have been decalcified with either HCl decal or formic acid decal. >> Before routine processing the bones appear to be decalcified, but at >> sectioning >> they are very hard and brittle (even after surface decal) and chip out >> of >> the >> paraffin block. >> Any suggestions would be helpful. A pathologist suggested a soap >> soaking >> solution but could not remember the name of the soap. >> >> Help and thank you, >> >> R. Berger, HT >> >> >> >> >> **************Looking for a car that's sporty, fun and fits in your >> budget? >> Read reviews on AOL Autos. >> ( >> http://autos.aol.com/cars-BMW-128-2008/expert-review?ncid=aolaut00050000000017) >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > Louise Renton > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > "There are nights when the wolves are silent and only the moon howls". > George Carlin > No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > No virus found in this incoming message. > Checked by AVG - http://www.avg.com > Version: 8.0.138 / Virus Database: 270.5.12/1594 - Release Date: 8/5/2008 > 9:49 PM > > > From SDrew <@t> uwhealth.org Wed Aug 6 07:33:31 2008 From: SDrew <@t> uwhealth.org (Drew Sally A.) Date: Wed Aug 6 07:33:41 2008 Subject: [Histonet] Factor XIIIa antibody In-Reply-To: <200808061214847.SM00940@csls2816> Message-ID: <3F328377AF4E23438E78234752652CE105D52772@uwhis-xchng7.uwhis.hosp.wisc.edu> I'f you are at all interested in a polyclonal antibody, we have been happy with BioCare Medical's F13a antibody, and I would be happy to tell you more about it if you desire. Sally Ann Drew, MT (ASCP) sdrew@uwhealth.org IHC/ISH Lab DB1-223, Mail Code 3224 600 Highland Ave. Madison, WI 53792 Phone (608) 265-6596 Fax (608) 262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Young Kwun Sent: Tuesday, August 05, 2008 9:15 PM To: Histonet Subject: [Histonet] Factor XIIIa antibody Hello All, We've been using Factor XIII, A-subunit polyclonal antibody from Calbiochem (Cat.No.233498) on skin conditions successfully so far. However this antibody was discontinued few years ago and they are offering Factor XIII, S-subunit only. Although I haven't tried FXIII S-unit immunostaining on human skin, I tried two other monoclonal Factor XIIIa but the stains were not as good as our current one. I tried Neomaker and Novocastra's. Can anyone suggest the source of good FXIIIa please? Thank you. Young Kwun Senior Hospital Scientist Dept. of Anatomical Pathology Concord Hospital Concord NSW 2139 Australia Tel)61-2-9767-6075 Fax)61-2-9767-8427 kwuny@email.cs.nsw.gov.au _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jdhisto <@t> yahoo.com Wed Aug 6 07:46:44 2008 From: jdhisto <@t> yahoo.com (JD) Date: Wed Aug 6 07:46:54 2008 Subject: [Histonet] Retic stain stat Message-ID: Hello all, I am trying to figure a substitute for a 2% ferric ammonium sulfate solution used in "gomori Retic" stain. Any and all comments welcome. This is an urgent mater especially since our ordering tech is a little behind on ordering and our Dr. needs this stat. Once again, thanks to all. JDhisto From Jackie.O'Connor <@t> abbott.com Wed Aug 6 08:07:55 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed Aug 6 08:08:28 2008 Subject: [Histonet] Horse bones In-Reply-To: <006801c8f7a0$67fd9840$d175be7c@pentium4> Message-ID: Consider your processing schedule as well. Is it long enough to infiltrate large bones? "Piero Nelva" Sent by: histonet-bounces@lists.utsouthwestern.edu 08/06/2008 03:42 AM To cc Subject Re: [Histonet] Horse bones For tough or brittle, but not necessarily calcified tissue, I find that soaking the block on a water soaked tissue for 10-15 minutes effective for softening. It can sometimes take all day to get a section, as trimming sometimes has to be done 4-5 microns at a time!! Piero Nelva Monash Medical Centre Victoria Australia ----- Original Message ----- From: "louise renton" To: ; Sent: Wednesday, August 06, 2008 5:58 PM Subject: Re: [Histonet] Horse bones > Some things to think about.... > > Rinse the tissue well with running tap water after decal and before > processing - this seems to make a difference (depends on the size of the > bone - 2hrs to overnight) > > EMbed the bones so that the long axis is either at right angles or 45 > degrees to the blade so that the edge of the knife doesn't have to cut > through a narrow band of cortical bone along its entire length - this > causes > "skipping"(If this is unclear I can send you an attachment with a > diagramme) > > Once you have faced the blocks put the face down on ice in a -20 deg > freezer > overnight (chest type freezer). then remove the one ata time for > sectioning. > > I have only had limited sucess with soaking the faced blocks in fabric > softener undiluted. > > Hopes this helps > > best regards > > > On 8/6/08, BERGERJL@aol.com wrote: >> >> We are trying to section pieces of horse femurs, fetlock and >> carpal joints. >> They have been decalcified with either HCl decal or formic acid decal. >> Before routine processing the bones appear to be decalcified, but at >> sectioning >> they are very hard and brittle (even after surface decal) and chip out >> of >> the >> paraffin block. >> Any suggestions would be helpful. A pathologist suggested a soap >> soaking >> solution but could not remember the name of the soap. >> >> Help and thank you, >> >> R. Berger, HT >> >> >> >> >> **************Looking for a car that's sporty, fun and fits in your >> budget? >> Read reviews on AOL Autos. >> ( >> http://autos.aol.com/cars-BMW-128-2008/expert-review?ncid=aolaut00050000000017 ) >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > Louise Renton > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > "There are nights when the wolves are silent and only the moon howls". > George Carlin > No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > No virus found in this incoming message. > Checked by AVG - http://www.avg.com > Version: 8.0.138 / Virus Database: 270.5.12/1594 - Release Date: 8/5/2008 > 9:49 PM > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tanisha.mcknight <@t> covance.com Wed Aug 6 08:15:52 2008 From: tanisha.mcknight <@t> covance.com (McKnight, Tanisha) Date: Wed Aug 6 08:16:09 2008 Subject: [Histonet] RE: Cost per test (Kari Breal) Message-ID: <816E3C72F855F14985FC31D7C963AE6F08E0344C@indexch03.ent.covance.com> Hi Rene J Buesa: Can I get a copy of your article as well? I'd sincerely appreciate it. Tanisha McKnight, HT(ASCP) AP-Histology/Specimen Management Covance CLS, Indianapolis -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, August 06, 2008 8:36 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 57, Issue 10 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Cost per test (Kari Breal) 2. HT Exam clarification (Marilyn Gamble) 3. RE: knife sharpener lapping plate (Hannen, Valerie) 4. Quick dry coverslipping media (Tom McNemar) 5. question regarding crystal violet stain...... (Elizabeth A. Allen) 6. RE: Quick dry coverslipping media (Rathborne, Toni) 7. Alcian blue/PAS (zodiac29@comcast.net) 8. RE: Quick dry coverslipping media (Blazek, Linda) 9. RE: Quick dry coverslipping media (Weems, Joyce) 10. RE: Quick dry coverslipping media (Blazek, Linda) 11. RE: Quick dry coverslipping media (Rathborne, Toni) 12. Re: Histonet Digest, Vol 57, Issue 9 (Pumla Pamla-Gutter) 13. Re: Cost per test (Rene J Buesa) 14. computer system volunteers? (Michael Mihalik) 15. Horse bones (BERGERJL@aol.com) 16. Factor XIIIa antibody (Young Kwun) 17. RE: surveillance cameras in the lab (Tony Henwood) 18. RE: Factor XIIIa antibody (Tony Henwood) 19. Re: RE: [Histonet] Factor XIIIa antibody (Steven Hacker) 20. Re: Horse bones (louise renton) 21. Re: Horse bones (Piero Nelva) 22. RE: Factor XIIIa antibody (Drew Sally A.) ---------------------------------------------------------------------- Message: 1 Date: Tue, 5 Aug 2008 12:15:53 -0500 From: "Kari Breal" Subject: [Histonet] Cost per test To: histonet@lists.utsouthwestern.edu Message-ID: <20080805T121553Z_439500110000@alexian.net> Content-Type: text/plain; charset=utf-8 Does anyone have a cost per slide/block formula for histology work they would be willing to share? Thanks, Kari Breal Histology Supervisor Alexian Brothers Medical Center 847-437-5500 ext. 5155 Fax 847-981-2023 brealk@alexian.net CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 2 Date: Tue, 5 Aug 2008 13:19:50 -0400 From: "Marilyn Gamble" Subject: [Histonet] HT Exam clarification To: "histonet" Message-ID: <380-22008825171950968@earthlink.net> Content-Type: text/plain; charset=US-ASCII The certification criteria is listed below. Under Route 2, please note the last sentence. If the applicant is under the supervision of a BOR, board certified technician/technologist, the supervisor can sign the attestation form to validate the experience. Explanation: In the case of Histopathology, the (board certified) supervisor is recognized as an "appropriately board certified medical scientist." Criteria: Histotechnician, HT(ASCP) To be eligible for this examination category, an applicant must satisfy the requirements of at least one of the following routes: Route 1: Successful completion of a NAACLS accredited Histotechnician program within the last 5 years prior to the date of application for examination; or Route 2: Associate degree or at least 60 semester hours (90 quarter hours) of academic credit from a regionally accredited college/university, with a combination of 12 semester hours (18 quarter hours) of biology and chemistry, AND one year full time acceptable experience in histopathology in the U.S., Canada or a CAP/The Joint Commission (JCAHO)/AABB accredited laboratory within the last ten years under the supervision of a pathologist (certified by the American Board of Pathology in Anatomic Pathology), or an appropriately board certified medical scientist. Laboratory Experience To fulfill the experience requirement for the Histotechnician examination, you must have experience, within the last ten years, in the following areas: Fixation Microtomy Processing Staining Marilyn Gamble marilynng@earthlink.net ------------------------------ Message: 3 Date: Tue, 5 Aug 2008 13:29:45 -0400 From: "Hannen, Valerie" Subject: RE: [Histonet] knife sharpener lapping plate To: "DiCarlo, Margaret" , Message-ID: <5680DA93771F0C48954CC8D38425E72401AB34DF@ISMAIL.parrishmed.local> Content-Type: text/plain; charset="us-ascii" We replace oour lapping plates when they get so thin that they can not be properly "resurfaced" or honed. If they slip off of each other when they are being honed...they are too thin. Valerie Hannen, MLT(ASCP),HTL,SU(FL) Parrish Medical Center Titusville,Florida -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DiCarlo, Margaret Sent: Friday, August 01, 2008 8:24 AM To: histonet@pathology.swmed.edu Subject: [Histonet] knife sharpener lapping plate Histonetters, For those of you who still use the Shandon Autosharp 5 for knife sharpening like me, how do you know when you need to replace the lapping plates? Thanks. Peggy DiCarlo (HT) Ortho Bone Lab Buffalo General Hospital 716-859-1293 2007 Best Places to Work Finalist Visit our careers page at www.kaleidahealth.org/careers CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************************************************** ********************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you ------------------------------ Message: 4 Date: Tue, 5 Aug 2008 13:32:49 -0400 From: "Tom McNemar" Subject: [Histonet] Quick dry coverslipping media To: Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F5A6@lmhsmail.lmhealth.org> Content-Type: text/plain; charset="iso-8859-1" We are being buried in slides. We are currently using Cytoseal 60 from Richard Alan as a coverslipping media but it takes a long time for it to dry. Does anyone knkow of a coverslipping media that would allow us to file our slides within a couple of days without them sticking together? Thanks! Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ------------------------------ Message: 5 Date: 05 Aug 2008 13:38:37 -0400 From: Elizabeth.A.Allen@Hitchcock.ORG (Elizabeth A. Allen) Subject: [Histonet] question regarding crystal violet stain...... To: histonet@lists.utsouthwestern.edu Message-ID: <42188051@mailbox4.Hitchcock.ORG> Content-Type: text/plain; charset=iso-8859-1 I am trying to find a procedure for staining fresh muscle tissue for crystal violet to demonstrate amyloid. Does anyone have a protocol they could send me? Thank you. Beth ------------------------------ Message: 6 Date: Tue, 5 Aug 2008 13:41:56 -0400 From: "Rathborne, Toni" Subject: RE: [Histonet] Quick dry coverslipping media To: "Tom McNemar" , Message-ID: Content-Type: text/plain; charset="utf-8" We use Richard-Allan mounting medium and file slides the next day. Catalog #4111 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Tom McNemar Sent: Tuesday, August 05, 2008 1:33 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Quick dry coverslipping media We are being buried in slides. We are currently using Cytoseal 60 from Richard Alan as a coverslipping media but it takes a long time for it to dry. Does anyone knkow of a coverslipping media that would allow us to file our slides within a couple of days without them sticking together? Thanks! Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. ------------------------------ Message: 7 Date: Tue, 05 Aug 2008 17:48:05 +0000 From: zodiac29@comcast.net Subject: [Histonet] Alcian blue/PAS To: histonet@lists.utsouthwestern.edu Message-ID: <080520081748.21750.48989255000CB90F000054F62216554886C7CD0C0E070B0196@comca st.net> Content-Type: text/plain Hello All, I wanted to know what variation of the alcian blue/PAS stain that you like best. We are going to start doing this stain instead of the alcian blue that we do now. The texts that I use (Carson, Bancroft, AFIP) all have diffrent variations. I just wanted some imput on what is best. Thank you Jenny ------------------------------ Message: 8 Date: Tue, 5 Aug 2008 13:57:58 -0400 From: "Blazek, Linda" Subject: [Histonet] RE: Quick dry coverslipping media To: "'Rathborne, Toni'" , Tom McNemar , "histonet@pathology.swmed.edu" Message-ID: <5A2BD13465E061429D6455C8D6B40E390B77E35D@IBMB7Exchange.digestivespecialists .com> Content-Type: text/plain; charset="us-ascii" I use that as well and have no problem with next day filing. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, August 05, 2008 1:42 PM To: Tom McNemar; histonet@pathology.swmed.edu Subject: RE: [Histonet] Quick dry coverslipping media We use Richard-Allan mounting medium and file slides the next day. Catalog #4111 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Tom McNemar Sent: Tuesday, August 05, 2008 1:33 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Quick dry coverslipping media We are being buried in slides. We are currently using Cytoseal 60 from Richard Alan as a coverslipping media but it takes a long time for it to dry. Does anyone knkow of a coverslipping media that would allow us to file our slides within a couple of days without them sticking together? Thanks! Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. ------------------------------ Message: 9 Date: Tue, 5 Aug 2008 13:52:25 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] Quick dry coverslipping media To: "Rathborne, Toni" , "Tom McNemar" , Message-ID: <982A0A9461F9BF438C7B19A6E425A3834AFB0D@ITSSSXM01V6.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" Does it work well with automatic coverslippers? Thanks, j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, August 05, 2008 1:42 PM To: Tom McNemar; histonet@pathology.swmed.edu Subject: RE: [Histonet] Quick dry coverslipping media We use Richard-Allan mounting medium and file slides the next day. Catalog #4111 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Tom McNemar Sent: Tuesday, August 05, 2008 1:33 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Quick dry coverslipping media We are being buried in slides. We are currently using Cytoseal 60 from Richard Alan as a coverslipping media but it takes a long time for it to dry. Does anyone knkow of a coverslipping media that would allow us to file our slides within a couple of days without them sticking together? Thanks! Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ------------------------------ Message: 10 Date: Tue, 5 Aug 2008 14:10:17 -0400 From: "Blazek, Linda" Subject: RE: [Histonet] Quick dry coverslipping media To: "'Weems, Joyce'" , "Rathborne, Toni" , Tom McNemar , "histonet@pathology.swmed.edu" Message-ID: <5A2BD13465E061429D6455C8D6B40E390B77E35F@IBMB7Exchange.digestivespecialists .com> Content-Type: text/plain; charset="us-ascii" I use it with my coverslipper. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Tuesday, August 05, 2008 1:52 PM To: Rathborne, Toni; Tom McNemar; histonet@pathology.swmed.edu Subject: RE: [Histonet] Quick dry coverslipping media Does it work well with automatic coverslippers? Thanks, j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, August 05, 2008 1:42 PM To: Tom McNemar; histonet@pathology.swmed.edu Subject: RE: [Histonet] Quick dry coverslipping media We use Richard-Allan mounting medium and file slides the next day. Catalog #4111 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Tom McNemar Sent: Tuesday, August 05, 2008 1:33 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Quick dry coverslipping media We are being buried in slides. We are currently using Cytoseal 60 from Richard Alan as a coverslipping media but it takes a long time for it to dry. Does anyone knkow of a coverslipping media that would allow us to file our slides within a couple of days without them sticking together? Thanks! Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Tue, 5 Aug 2008 14:03:09 -0400 From: "Rathborne, Toni" Subject: RE: [Histonet] Quick dry coverslipping media To: "Weems, Joyce" , "Tom McNemar" , Message-ID: Content-Type: text/plain; charset="utf-8" Yes. We use it on our Leica CV5030. -----Original Message----- From: Weems, Joyce [mailto:JWeems@sjha.org] Sent: Tuesday, August 05, 2008 1:52 PM To: Rathborne, Toni; Tom McNemar; histonet@pathology.swmed.edu Subject: RE: [Histonet] Quick dry coverslipping media Does it work well with automatic coverslippers? Thanks, j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, August 05, 2008 1:42 PM To: Tom McNemar; histonet@pathology.swmed.edu Subject: RE: [Histonet] Quick dry coverslipping media We use Richard-Allan mounting medium and file slides the next day. Catalog #4111 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Tom McNemar Sent: Tuesday, August 05, 2008 1:33 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Quick dry coverslipping media We are being buried in slides. We are currently using Cytoseal 60 from Richard Alan as a coverslipping media but it takes a long time for it to dry. Does anyone knkow of a coverslipping media that would allow us to file our slides within a couple of days without them sticking together? Thanks! Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. ------------------------------ Message: 12 Date: Tue, 5 Aug 2008 14:50:03 -0400 From: "Pumla Pamla-Gutter" Subject: [Histonet] Re: Histonet Digest, Vol 57, Issue 9 To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Dear All, Any feedback appreciated. I've always used Cresyl Violet Echt, and now I've just switched to Cresyl Violet acetate. Does anyone know if I could treat these two the same way? I've been using 5g of the cresyl in 1000ml of dH2O. Does anyone work with this Nissl body stain and if so what protocol do you use? Thank you. Pumla ------------------------------ Message: 13 Date: Tue, 5 Aug 2008 13:21:53 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Cost per test To: histonet@lists.utsouthwestern.edu, Kari Breal Message-ID: <286694.63074.qm@web65704.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Under separate cover I am sending you an article I wrote on the subject. Rene J. --- On Tue, 8/5/08, Kari Breal wrote: From: Kari Breal Subject: [Histonet] Cost per test To: histonet@lists.utsouthwestern.edu Date: Tuesday, August 5, 2008, 1:15 PM Does anyone have a cost per slide/block formula for histology work they would be willing to share? Thanks, Kari Breal Histology Supervisor Alexian Brothers Medical Center 847-437-5500 ext. 5155 Fax 847-981-2023 brealk@alexian.net CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Tue, 5 Aug 2008 17:41:43 -0400 From: "Michael Mihalik" Subject: [Histonet] computer system volunteers? To: Message-ID: <00bb01c8f744$1f138970$5d3a9c50$@com> Content-Type: text/plain; charset="us-ascii" Good evening, I'm looking for some volunteers to give us some input on billing features that you absolutely love or would love to have in your system. Basically, we're enhancing the billing aspect of our AP system and we'd like to get as many opinions as possible. Of course, we've already started with our current clients, but we'd like to expand our horizons. So, if you don't mind sharing some of the best parts, worst parts, or even fantasies, I'd appreciate it. To start with, our overall objective is to make billing as automatic as possible. Why we involve our technologists or pathologists in billing has always been beyond me. Your jobs should be to focus on performing the task as hand, and to me, at least, that's not about billing. ...though I know we have to bill to stay in existence. I appreciate whatever you can give me. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pumla Pamla-Gutter Sent: Tuesday, August 05, 2008 2:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 57, Issue 9 Dear All, Any feedback appreciated. I've always used Cresyl Violet Echt, and now I've just switched to Cresyl Violet acetate. Does anyone know if I could treat these two the same way? I've been using 5g of the cresyl in 1000ml of dH2O. Does anyone work with this Nissl body stain and if so what protocol do you use? Thank you. Pumla _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Tue, 5 Aug 2008 20:07:50 EDT From: BERGERJL@aol.com Subject: [Histonet] Horse bones To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" We are trying to section pieces of horse femurs, fetlock and carpal joints. They have been decalcified with either HCl decal or formic acid decal. Before routine processing the bones appear to be decalcified, but at sectioning they are very hard and brittle (even after surface decal) and chip out of the paraffin block. Any suggestions would be helpful. A pathologist suggested a soap soaking solution but could not remember the name of the soap. Help and thank you, R. Berger, HT **************Looking for a car that's sporty, fun and fits in your budget? Read reviews on AOL Autos. (http://autos.aol.com/cars-BMW-128-2008/expert-review?ncid=aolaut00050000000 017 ) ------------------------------ Message: 16 Date: Wed, 6 Aug 2008 12:14:36 +1000 From: "Young Kwun" Subject: [Histonet] Factor XIIIa antibody To: "Histonet" Message-ID: <200808061214847.SM00940@csls2816> Content-Type: text/plain; charset="us-ascii" Hello All, We've been using Factor XIII, A-subunit polyclonal antibody from Calbiochem (Cat.No.233498) on skin conditions successfully so far. However this antibody was discontinued few years ago and they are offering Factor XIII, S-subunit only. Although I haven't tried FXIII S-unit immunostaining on human skin, I tried two other monoclonal Factor XIIIa but the stains were not as good as our current one. I tried Neomaker and Novocastra's. Can anyone suggest the source of good FXIIIa please? Thank you. Young Kwun Senior Hospital Scientist Dept. of Anatomical Pathology Concord Hospital Concord NSW 2139 Australia Tel)61-2-9767-6075 Fax)61-2-9767-8427 kwuny@email.cs.nsw.gov.au ------------------------------ Message: 17 Date: Wed, 6 Aug 2008 14:05:56 +1000 From: "Tony Henwood" Subject: RE: [Histonet] surveillance cameras in the lab To: "Bill" , , Message-ID: Content-Type: text/plain; charset="us-ascii" If you like, I can come and clean the lenses, at a modest price (say 10c/hr) plus travelling. My microscope lenses always look worse after I clean them, so I should have the same success with your camera lenses. By the way, I live in Sydney, Australia!! But seriously, what a dumb move, to install cameras without consultation!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bill Sent: Tuesday, 5 August 2008 12:02 AM To: Pathrm35@comcast.net; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] surveillance cameras in the lab At 10:21 AM +0000 8/4/08, Pathrm35@comcast.net wrote: >>>I was wondering how many techs out there have cameras in their labs, >>>either for security or to monitor employees. I went to work Sunday >>>night and noticed that 4 cameras were installed in the lab over the >>>weekend, with more to come.<<< I would use immersion oil on the camera lenses. -- ______________ Bill Blank, MD Heartland Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 18 Date: Wed, 6 Aug 2008 14:31:10 +1000 From: "Tony Henwood" Subject: RE: [Histonet] Factor XIIIa antibody To: "Young Kwun" , "Histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Young, How is my old lab going? Try Biogenix Cat No: MU337-UP Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Young Kwun Sent: Wednesday, 6 August 2008 12:15 PM To: Histonet Subject: [Histonet] Factor XIIIa antibody Hello All, We've been using Factor XIII, A-subunit polyclonal antibody from Calbiochem (Cat.No.233498) on skin conditions successfully so far. However this antibody was discontinued few years ago and they are offering Factor XIII, S-subunit only. Although I haven't tried FXIII S-unit immunostaining on human skin, I tried two other monoclonal Factor XIIIa but the stains were not as good as our current one. I tried Neomaker and Novocastra's. Can anyone suggest the source of good FXIIIa please? Thank you. Young Kwun Senior Hospital Scientist Dept. of Anatomical Pathology Concord Hospital Concord NSW 2139 Australia Tel)61-2-9767-6075 Fax)61-2-9767-8427 kwuny@email.cs.nsw.gov.au _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 19 Date: Tue, 05 Aug 2008 23:43:32 -0500 (CDT) From: Steven Hacker Subject: Re: RE: [Histonet] Factor XIIIa antibody To: Tony Henwood , Histonet , Young Kwun Message-ID: <18418115.7124031217997812488.JavaMail.root@vms068.mailsrvcs.net> Content-Type: text/plain; charset=UTF-8 Please take a look at BioCare Medical's Factor XIIIa Ab... ===================== From: Tony Henwood Date: 2008/08/06 Wed AM 12:31:10 EDT To: Young Kwun , Histonet Subject: RE: [Histonet] Factor XIIIa antibody Hi Young, How is my old lab going? Try Biogenix Cat No: MU337-UP Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Young Kwun Sent: Wednesday, 6 August 2008 12:15 PM To: Histonet Subject: [Histonet] Factor XIIIa antibody Hello All, We've been using Factor XIII, A-subunit polyclonal antibody from Calbiochem (Cat.No.233498) on skin conditions successfully so far. However this antibody was discontinued few years ago and they are offering Factor XIII, S-subunit only. Although I haven't tried FXIII S-unit immunostaining on human skin, I tried two other monoclonal Factor XIIIa but the stains were not as good as our current one. I tried Neomaker and Novocastra's. Can anyone suggest the source of good FXIIIa please? Thank you. Young Kwun Senior Hospital Scientist Dept. of Anatomical Pathology Concord Hospital Concord NSW 2139 Australia Tel)61-2-9767-6075 Fax)61-2-9767-8427 kwuny@email.cs.nsw.gov.au _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Wed, 6 Aug 2008 09:58:41 +0200 From: "louise renton" Subject: Re: [Histonet] Horse bones To: "BERGERJL@aol.com" , Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Some things to think about.... Rinse the tissue well with running tap water after decal and before processing - this seems to make a difference (depends on the size of the bone - 2hrs to overnight) EMbed the bones so that the long axis is either at right angles or 45 degrees to the blade so that the edge of the knife doesn't have to cut through a narrow band of cortical bone along its entire length - this causes "skipping"(If this is unclear I can send you an attachment with a diagramme) Once you have faced the blocks put the face down on ice in a -20 deg freezer overnight (chest type freezer). then remove the one ata time for sectioning. I have only had limited sucess with soaking the faced blocks in fabric softener undiluted. Hopes this helps best regards On 8/6/08, BERGERJL@aol.com wrote: > > We are trying to section pieces of horse femurs, fetlock and > carpal joints. > They have been decalcified with either HCl decal or formic acid decal. > Before routine processing the bones appear to be decalcified, but at > sectioning > they are very hard and brittle (even after surface decal) and chip out of > the > paraffin block. > Any suggestions would be helpful. A pathologist suggested a soap soaking > solution but could not remember the name of the soap. > > Help and thank you, > > R. Berger, HT > > > > > **************Looking for a car that's sporty, fun and fits in your budget? > Read reviews on AOL Autos. > ( > http://autos.aol.com/cars-BMW-128-2008/expert-review?ncid=aolaut000500000000 17) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ------------------------------ Message: 21 Date: Wed, 6 Aug 2008 18:42:48 +1000 From: "Piero Nelva" Subject: Re: [Histonet] Horse bones To: Message-ID: <006801c8f7a0$67fd9840$d175be7c@pentium4> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original For tough or brittle, but not necessarily calcified tissue, I find that soaking the block on a water soaked tissue for 10-15 minutes effective for softening. It can sometimes take all day to get a section, as trimming sometimes has to be done 4-5 microns at a time!! Piero Nelva Monash Medical Centre Victoria Australia ----- Original Message ----- From: "louise renton" To: ; Sent: Wednesday, August 06, 2008 5:58 PM Subject: Re: [Histonet] Horse bones > Some things to think about.... > > Rinse the tissue well with running tap water after decal and before > processing - this seems to make a difference (depends on the size of the > bone - 2hrs to overnight) > > EMbed the bones so that the long axis is either at right angles or 45 > degrees to the blade so that the edge of the knife doesn't have to cut > through a narrow band of cortical bone along its entire length - this > causes > "skipping"(If this is unclear I can send you an attachment with a > diagramme) > > Once you have faced the blocks put the face down on ice in a -20 deg > freezer > overnight (chest type freezer). then remove the one ata time for > sectioning. > > I have only had limited sucess with soaking the faced blocks in fabric > softener undiluted. > > Hopes this helps > > best regards > > > On 8/6/08, BERGERJL@aol.com wrote: >> >> We are trying to section pieces of horse femurs, fetlock and >> carpal joints. >> They have been decalcified with either HCl decal or formic acid decal. >> Before routine processing the bones appear to be decalcified, but at >> sectioning >> they are very hard and brittle (even after surface decal) and chip out >> of >> the >> paraffin block. >> Any suggestions would be helpful. A pathologist suggested a soap >> soaking >> solution but could not remember the name of the soap. >> >> Help and thank you, >> >> R. Berger, HT >> >> >> >> >> **************Looking for a car that's sporty, fun and fits in your >> budget? >> Read reviews on AOL Autos. >> ( >> http://autos.aol.com/cars-BMW-128-2008/expert-review?ncid=aolaut000500000000 17) >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > Louise Renton > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > "There are nights when the wolves are silent and only the moon howls". > George Carlin > No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > No virus found in this incoming message. > Checked by AVG - http://www.avg.com > Version: 8.0.138 / Virus Database: 270.5.12/1594 - Release Date: 8/5/2008 > 9:49 PM > > > ------------------------------ Message: 22 Date: Wed, 6 Aug 2008 07:33:31 -0500 From: "Drew Sally A." Subject: RE: [Histonet] Factor XIIIa antibody To: "Histonet" Message-ID: <3F328377AF4E23438E78234752652CE105D52772@uwhis-xchng7.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="US-ASCII" I'f you are at all interested in a polyclonal antibody, we have been happy with BioCare Medical's F13a antibody, and I would be happy to tell you more about it if you desire. Sally Ann Drew, MT (ASCP) sdrew@uwhealth.org IHC/ISH Lab DB1-223, Mail Code 3224 600 Highland Ave. Madison, WI 53792 Phone (608) 265-6596 Fax (608) 262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Young Kwun Sent: Tuesday, August 05, 2008 9:15 PM To: Histonet Subject: [Histonet] Factor XIIIa antibody Hello All, We've been using Factor XIII, A-subunit polyclonal antibody from Calbiochem (Cat.No.233498) on skin conditions successfully so far. However this antibody was discontinued few years ago and they are offering Factor XIII, S-subunit only. Although I haven't tried FXIII S-unit immunostaining on human skin, I tried two other monoclonal Factor XIIIa but the stains were not as good as our current one. I tried Neomaker and Novocastra's. Can anyone suggest the source of good FXIIIa please? Thank you. Young Kwun Senior Hospital Scientist Dept. of Anatomical Pathology Concord Hospital Concord NSW 2139 Australia Tel)61-2-9767-6075 Fax)61-2-9767-8427 kwuny@email.cs.nsw.gov.au _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 57, Issue 10 **************************************** ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From galinadeyneko <@t> yahoo.com Wed Aug 6 09:58:52 2008 From: galinadeyneko <@t> yahoo.com (Galina Deyneko) Date: Wed Aug 6 09:58:55 2008 Subject: [Histonet] (no subject) Message-ID: <400499.98067.qm@web33107.mail.mud.yahoo.com> Hi Subat, I like the following protocol for mouse hearts ( i received it from James Watson and modified little bit). 70 % Ethanol - optional ( we keep the tissue in 70 % some times coulple days before processing in cold room at 4C) 80% , 95%, 95%- 45 min each run, wacuum off 100%- 45 min 100% with glycerol(5% solution of glycerol)- 45 min 100% with glycerol(5% solution of glycerol)- 45 min Xylene -30 min, Xylene -45 min, Xylene - 45 min Paraffin 1, paraffin II, Paraffin III- 45 min each. Started from 100 % the wacuum is on. We use snandon processing center. I am not alone who use a processor that is why we exclude Glycerol, but I am sure that this reagent is goog for processing of the hearts. Be very patient during the cutting and check the sections in microscope. I soak the specimens in the ice before cutting and you can add fabric softener or several drops Tween 20 into the ice.I like advice to soak a few seconds the trimmed block in hot water before placed on ice. Even you can soak 5 minutes in DI water at RT, but be very careful with the first section, the tissue protrudes?after soaking.?Also we use "intermediate" water bath with DI water with RT and place the sections in this water bath first and after transfer on glass slide to hot water bath. Also you can soak with cold water the surface of the block using finger, and I like to breath on the surface of the block before each section, breathing gives some additional moisture to surface and facilitates cutting. We use two type of paraffin for processing and embedding from Surgipath. Also i can give the good reference for paraffin "Shandon precision cut" Thermo Shandon cat # B1002490. Best regards galina deyneko Novartis Cambridge MA ? ? ? From SAllen <@t> exchange.hsc.mb.ca Wed Aug 6 10:25:23 2008 From: SAllen <@t> exchange.hsc.mb.ca (Sharon Allen) Date: Wed Aug 6 10:25:27 2008 Subject: [Histonet] Epitomics ErbB2/HER2, clone SP3 Message-ID: Hi, Can anyone tell me what dilution and antigen retrieval method they are using for this product? I know the company suggests 1:100 but we usually find we end up with something different. Thanks, Debbie Watson, Health Sciences Centre, Winnipeg, Manitoba -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From CBark <@t> memorialcare.org Wed Aug 6 10:36:17 2008 From: CBark <@t> memorialcare.org (Christine Bark) Date: Wed Aug 6 10:36:42 2008 Subject: [Histonet] RE: Quick dry coverslipping media In-Reply-To: <6487443D1P81972579-01@emf1.memorialcare.org> Message-ID: We use Mounting Medium (MM) 24 from Surgipath on our automated coverslipper and for hand coverslipping. It works great. Christine Bark HT(ASCP) Lead Histotech, Pathology Saddleback Memorial Medical Center 949-452-3548 cbark@memorialcare.org -----Original Message----- We are being buried in slides. We are currently using Cytoseal 60 from Richard Alan as a coverslipping media but it takes a long time for it to dry. Does anyone knkow of a coverslipping media that would allow us to file our slides within a couple of days without them sticking together? Thanks! Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org ------------------------------ Message: 5 Date: 05 Aug 2008 13:38:37 -0400 From: Elizabeth.A.Allen@Hitchcock.ORG (Elizabeth A. Allen) Subject: [Histonet] question regarding crystal violet stain...... To: histonet@lists.utsouthwestern.edu Message-ID: <42188051@mailbox4.Hitchcock.ORG> Content-Type: text/plain; charset=iso-8859-1 I am trying to find a procedure for staining fresh muscle tissue for crystal violet to demonstrate amyloid. Does anyone have a protocol they could send me? Thank you. Beth ------------------------------ Message: 6 Date: Tue, 5 Aug 2008 13:41:56 -0400 From: "Rathborne, Toni" Subject: RE: [Histonet] Quick dry coverslipping media To: "Tom McNemar" , Message-ID: Content-Type: text/plain; charset="utf-8" We use Richard-Allan mounting medium and file slides the next day. Catalog #4111 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Tom McNemar Sent: Tuesday, August 05, 2008 1:33 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Quick dry coverslipping media We are being buried in slides. We are currently using Cytoseal 60 from Richard Alan as a coverslipping media but it takes a long time for it to dry. Does anyone knkow of a coverslipping media that would allow us to file our slides within a couple of days without them sticking together? Thanks! Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. ------------------------------ Message: 7 Date: Tue, 05 Aug 2008 17:48:05 +0000 From: zodiac29@comcast.net Subject: [Histonet] Alcian blue/PAS To: histonet@lists.utsouthwestern.edu Message-ID: <080520081748.21750.48989255000CB90F000054F62216554886C7CD0C0E070B0196@comcast.net> Content-Type: text/plain Hello All, I wanted to know what variation of the alcian blue/PAS stain that you like best. We are going to start doing this stain instead of the alcian blue that we do now. The texts that I use (Carson, Bancroft, AFIP) all have diffrent variations. I just wanted some imput on what is best. Thank you Jenny ------------------------------ Message: 8 Date: Tue, 5 Aug 2008 13:57:58 -0400 From: "Blazek, Linda" Subject: [Histonet] RE: Quick dry coverslipping media To: "'Rathborne, Toni'" , Tom McNemar , "histonet@pathology.swmed.edu" Message-ID: <5A2BD13465E061429D6455C8D6B40E390B77E35D@IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" I use that as well and have no problem with next day filing. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, August 05, 2008 1:42 PM To: Tom McNemar; histonet@pathology.swmed.edu Subject: RE: [Histonet] Quick dry coverslipping media We use Richard-Allan mounting medium and file slides the next day. Catalog #4111 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Tom McNemar Sent: Tuesday, August 05, 2008 1:33 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Quick dry coverslipping media We are being buried in slides. We are currently using Cytoseal 60 from Richard Alan as a coverslipping media but it takes a long time for it to dry. Does anyone knkow of a coverslipping media that would allow us to file our slides within a couple of days without them sticking together? Thanks! Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. ------------------------------ Message: 9 Date: Tue, 5 Aug 2008 13:52:25 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] Quick dry coverslipping media To: "Rathborne, Toni" , "Tom McNemar" , Message-ID: <982A0A9461F9BF438C7B19A6E425A3834AFB0D@ITSSSXM01V6.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" Does it work well with automatic coverslippers? Thanks, j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, August 05, 2008 1:42 PM To: Tom McNemar; histonet@pathology.swmed.edu Subject: RE: [Histonet] Quick dry coverslipping media We use Richard-Allan mounting medium and file slides the next day. Catalog #4111 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Tom McNemar Sent: Tuesday, August 05, 2008 1:33 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Quick dry coverslipping media We are being buried in slides. We are currently using Cytoseal 60 from Richard Alan as a coverslipping media but it takes a long time for it to dry. Does anyone knkow of a coverslipping media that would allow us to file our slides within a couple of days without them sticking together? Thanks! Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ------------------------------ Message: 10 Date: Tue, 5 Aug 2008 14:10:17 -0400 From: "Blazek, Linda" Subject: RE: [Histonet] Quick dry coverslipping media To: "'Weems, Joyce'" , "Rathborne, Toni" , Tom McNemar , "histonet@pathology.swmed.edu" Message-ID: <5A2BD13465E061429D6455C8D6B40E390B77E35F@IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" I use it with my coverslipper. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Tuesday, August 05, 2008 1:52 PM To: Rathborne, Toni; Tom McNemar; histonet@pathology.swmed.edu Subject: RE: [Histonet] Quick dry coverslipping media Does it work well with automatic coverslippers? Thanks, j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, August 05, 2008 1:42 PM To: Tom McNemar; histonet@pathology.swmed.edu Subject: RE: [Histonet] Quick dry coverslipping media We use Richard-Allan mounting medium and file slides the next day. Catalog #4111 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Tom McNemar Sent: Tuesday, August 05, 2008 1:33 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Quick dry coverslipping media We are being buried in slides. We are currently using Cytoseal 60 from Richard Alan as a coverslipping media but it takes a long time for it to dry. Does anyone knkow of a coverslipping media that would allow us to file our slides within a couple of days without them sticking together? Thanks! Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Tue, 5 Aug 2008 14:03:09 -0400 From: "Rathborne, Toni" Subject: RE: [Histonet] Quick dry coverslipping media To: "Weems, Joyce" , "Tom McNemar" , Message-ID: Content-Type: text/plain; charset="utf-8" Yes. We use it on our Leica CV5030. -----Original Message----- From: Weems, Joyce [mailto:JWeems@sjha.org] Sent: Tuesday, August 05, 2008 1:52 PM To: Rathborne, Toni; Tom McNemar; histonet@pathology.swmed.edu Subject: RE: [Histonet] Quick dry coverslipping media Does it work well with automatic coverslippers? Thanks, j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, August 05, 2008 1:42 PM To: Tom McNemar; histonet@pathology.swmed.edu Subject: RE: [Histonet] Quick dry coverslipping media We use Richard-Allan mounting medium and file slides the next day. Catalog #4111 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Tom McNemar Sent: Tuesday, August 05, 2008 1:33 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Quick dry coverslipping media We are being buried in slides. We are currently using Cytoseal 60 from Richard Alan as a coverslipping media but it takes a long time for it to dry. Does anyone knkow of a coverslipping media that would allow us to file our slides within a couple of days without them sticking together? Thanks! Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. ------------------------------ Message: 12 Date: Tue, 5 Aug 2008 14:50:03 -0400 From: "Pumla Pamla-Gutter" Subject: [Histonet] Re: Histonet Digest, Vol 57, Issue 9 To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Dear All, Any feedback appreciated. I've always used Cresyl Violet Echt, and now I've just switched to Cresyl Violet acetate. Does anyone know if I could treat these two the same way? I've been using 5g of the cresyl in 1000ml of dH2O. Does anyone work with this Nissl body stain and if so what protocol do you use? Thank you. Pumla ------------------------------ Message: 13 Date: Tue, 5 Aug 2008 13:21:53 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Cost per test To: histonet@lists.utsouthwestern.edu, Kari Breal Message-ID: <286694.63074.qm@web65704.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Under separate cover I am sending you an article I wrote on the subject. Ren? J. --- On Tue, 8/5/08, Kari Breal wrote: From: Kari Breal Subject: [Histonet] Cost per test To: histonet@lists.utsouthwestern.edu Date: Tuesday, August 5, 2008, 1:15 PM Does anyone have a cost per slide/block formula for histology work they would be willing to share? Thanks, Kari Breal Histology Supervisor Alexian Brothers Medical Center 847-437-5500 ext. 5155 Fax 847-981-2023 brealk@alexian.net CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Tue, 5 Aug 2008 17:41:43 -0400 From: "Michael Mihalik" Subject: [Histonet] computer system volunteers? To: Message-ID: <00bb01c8f744$1f138970$5d3a9c50$@com> Content-Type: text/plain; charset="us-ascii" Good evening, I'm looking for some volunteers to give us some input on billing features that you absolutely love or would love to have in your system. Basically, we're enhancing the billing aspect of our AP system and we'd like to get as many opinions as possible. Of course, we've already started with our current clients, but we'd like to expand our horizons. So, if you don't mind sharing some of the best parts, worst parts, or even fantasies, I'd appreciate it. To start with, our overall objective is to make billing as automatic as possible. Why we involve our technologists or pathologists in billing has always been beyond me. Your jobs should be to focus on performing the task as hand, and to me, at least, that's not about billing. ...though I know we have to bill to stay in existence. I appreciate whatever you can give me. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pumla Pamla-Gutter Sent: Tuesday, August 05, 2008 2:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 57, Issue 9 Dear All, Any feedback appreciated. I've always used Cresyl Violet Echt, and now I've just switched to Cresyl Violet acetate. Does anyone know if I could treat these two the same way? I've been using 5g of the cresyl in 1000ml of dH2O. Does anyone work with this Nissl body stain and if so what protocol do you use? Thank you. Pumla _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Tue, 5 Aug 2008 20:07:50 EDT From: BERGERJL@aol.com Subject: [Histonet] Horse bones To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" We are trying to section pieces of horse femurs, fetlock and carpal joints. They have been decalcified with either HCl decal or formic acid decal. Before routine processing the bones appear to be decalcified, but at sectioning they are very hard and brittle (even after surface decal) and chip out of the paraffin block. Any suggestions would be helpful. A pathologist suggested a soap soaking solution but could not remember the name of the soap. Help and thank you, R. Berger, HT **************Looking for a car that's sporty, fun and fits in your budget? Read reviews on AOL Autos. (http://autos.aol.com/cars-BMW-128-2008/expert-review?ncid=aolaut00050000000017 ) ------------------------------ Message: 16 Date: Wed, 6 Aug 2008 12:14:36 +1000 From: "Young Kwun" Subject: [Histonet] Factor XIIIa antibody To: "Histonet" Message-ID: <200808061214847.SM00940@csls2816> Content-Type: text/plain; charset="us-ascii" Hello All, We've been using Factor XIII, A-subunit polyclonal antibody from Calbiochem (Cat.No.233498) on skin conditions successfully so far. However this antibody was discontinued few years ago and they are offering Factor XIII, S-subunit only. Although I haven't tried FXIII S-unit immunostaining on human skin, I tried two other monoclonal Factor XIIIa but the stains were not as good as our current one. I tried Neomaker and Novocastra's. Can anyone suggest the source of good FXIIIa please? Thank you. Young Kwun Senior Hospital Scientist Dept. of Anatomical Pathology Concord Hospital Concord NSW 2139 Australia Tel)61-2-9767-6075 Fax)61-2-9767-8427 kwuny@email.cs.nsw.gov.au ------------------------------ Message: 17 Date: Wed, 6 Aug 2008 14:05:56 +1000 From: "Tony Henwood" Subject: RE: [Histonet] surveillance cameras in the lab To: "Bill" , , Message-ID: Content-Type: text/plain; charset="us-ascii" If you like, I can come and clean the lenses, at a modest price (say 10c/hr) plus travelling. My microscope lenses always look worse after I clean them, so I should have the same success with your camera lenses. By the way, I live in Sydney, Australia!! But seriously, what a dumb move, to install cameras without consultation!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bill Sent: Tuesday, 5 August 2008 12:02 AM To: Pathrm35@comcast.net; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] surveillance cameras in the lab At 10:21 AM +0000 8/4/08, Pathrm35@comcast.net wrote: >>>I was wondering how many techs out there have cameras in their labs, >>>either for security or to monitor employees. I went to work Sunday >>>night and noticed that 4 cameras were installed in the lab over the >>>weekend, with more to come.<<< I would use immersion oil on the camera lenses. -- ______________ Bill Blank, MD Heartland Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 18 Date: Wed, 6 Aug 2008 14:31:10 +1000 From: "Tony Henwood" Subject: RE: [Histonet] Factor XIIIa antibody To: "Young Kwun" , "Histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Young, How is my old lab going? Try Biogenix Cat No: MU337-UP Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Young Kwun Sent: Wednesday, 6 August 2008 12:15 PM To: Histonet Subject: [Histonet] Factor XIIIa antibody Hello All, We've been using Factor XIII, A-subunit polyclonal antibody from Calbiochem (Cat.No.233498) on skin conditions successfully so far. However this antibody was discontinued few years ago and they are offering Factor XIII, S-subunit only. Although I haven't tried FXIII S-unit immunostaining on human skin, I tried two other monoclonal Factor XIIIa but the stains were not as good as our current one. I tried Neomaker and Novocastra's. Can anyone suggest the source of good FXIIIa please? Thank you. Young Kwun Senior Hospital Scientist Dept. of Anatomical Pathology Concord Hospital Concord NSW 2139 Australia Tel)61-2-9767-6075 Fax)61-2-9767-8427 kwuny@email.cs.nsw.gov.au _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 19 Date: Tue, 05 Aug 2008 23:43:32 -0500 (CDT) From: Steven Hacker Subject: Re: RE: [Histonet] Factor XIIIa antibody To: Tony Henwood , Histonet , Young Kwun Message-ID: <18418115.7124031217997812488.JavaMail.root@vms068.mailsrvcs.net> Content-Type: text/plain; charset=UTF-8 Please take a look at BioCare Medical's Factor XIIIa Ab... ===================== From: Tony Henwood Date: 2008/08/06 Wed AM 12:31:10 EDT To: Young Kwun , Histonet Subject: RE: [Histonet] Factor XIIIa antibody Hi Young, How is my old lab going? Try Biogenix Cat No: MU337-UP Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Young Kwun Sent: Wednesday, 6 August 2008 12:15 PM To: Histonet Subject: [Histonet] Factor XIIIa antibody Hello All, We've been using Factor XIII, A-subunit polyclonal antibody from Calbiochem (Cat.No.233498) on skin conditions successfully so far. However this antibody was discontinued few years ago and they are offering Factor XIII, S-subunit only. Although I haven't tried FXIII S-unit immunostaining on human skin, I tried two other monoclonal Factor XIIIa but the stains were not as good as our current one. I tried Neomaker and Novocastra's. Can anyone suggest the source of good FXIIIa please? Thank you. Young Kwun Senior Hospital Scientist Dept. of Anatomical Pathology Concord Hospital Concord NSW 2139 Australia Tel)61-2-9767-6075 Fax)61-2-9767-8427 kwuny@email.cs.nsw.gov.au _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Wed, 6 Aug 2008 09:58:41 +0200 From: "louise renton" Subject: Re: [Histonet] Horse bones To: "BERGERJL@aol.com" , Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Some things to think about.... Rinse the tissue well with running tap water after decal and before processing - this seems to make a difference (depends on the size of the bone - 2hrs to overnight) EMbed the bones so that the long axis is either at right angles or 45 degrees to the blade so that the edge of the knife doesn't have to cut through a narrow band of cortical bone along its entire length - this causes "skipping"(If this is unclear I can send you an attachment with a diagramme) Once you have faced the blocks put the face down on ice in a -20 deg freezer overnight (chest type freezer). then remove the one ata time for sectioning. I have only had limited sucess with soaking the faced blocks in fabric softener undiluted. Hopes this helps best regards On 8/6/08, BERGERJL@aol.com wrote: > > We are trying to section pieces of horse femurs, fetlock and > carpal joints. > They have been decalcified with either HCl decal or formic acid decal. > Before routine processing the bones appear to be decalcified, but at > sectioning > they are very hard and brittle (even after surface decal) and chip out of > the > paraffin block. > Any suggestions would be helpful. A pathologist suggested a soap soaking > solution but could not remember the name of the soap. > > Help and thank you, > > R. Berger, HT > > > > > **************Looking for a car that's sporty, fun and fits in your budget? > Read reviews on AOL Autos. > ( > http://autos.aol.com/cars-BMW-128-2008/expert-review?ncid=aolaut00050000000017) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ------------------------------ Message: 21 Date: Wed, 6 Aug 2008 18:42:48 +1000 From: "Piero Nelva" Subject: Re: [Histonet] Horse bones To: Message-ID: <006801c8f7a0$67fd9840$d175be7c@pentium4> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original For tough or brittle, but not necessarily calcified tissue, I find that soaking the block on a water soaked tissue for 10-15 minutes effective for softening. It can sometimes take all day to get a section, as trimming sometimes has to be done 4-5 microns at a time!! Piero Nelva Monash Medical Centre Victoria Australia ----- Original Message ----- From: "louise renton" To: ; Sent: Wednesday, August 06, 2008 5:58 PM Subject: Re: [Histonet] Horse bones > Some things to think about.... > > Rinse the tissue well with running tap water after decal and before > processing - this seems to make a difference (depends on the size of the > bone - 2hrs to overnight) > > EMbed the bones so that the long axis is either at right angles or 45 > degrees to the blade so that the edge of the knife doesn't have to cut > through a narrow band of cortical bone along its entire length - this > causes > "skipping"(If this is unclear I can send you an attachment with a > diagramme) > > Once you have faced the blocks put the face down on ice in a -20 deg > freezer > overnight (chest type freezer). then remove the one ata time for > sectioning. > > I have only had limited sucess with soaking the faced blocks in fabric > softener undiluted. > > Hopes this helps > > best regards > > > On 8/6/08, BERGERJL@aol.com wrote: >> >> We are trying to section pieces of horse femurs, fetlock and >> carpal joints. >> They have been decalcified with either HCl decal or formic acid decal. >> Before routine processing the bones appear to be decalcified, but at >> sectioning >> they are very hard and brittle (even after surface decal) and chip out >> of >> the >> paraffin block. >> Any suggestions would be helpful. A pathologist suggested a soap >> soaking >> solution but could not remember the name of the soap. >> >> Help and thank you, >> >> R. Berger, HT >> >> >> >> >> **************Looking for a car that's sporty, fun and fits in your >> budget? >> Read reviews on AOL Autos. >> ( >> http://autos.aol.com/cars-BMW-128-2008/expert-review?ncid=aolaut00050000000017) >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > Louise Renton > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > "There are nights when the wolves are silent and only the moon howls". > George Carlin > No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > No virus found in this incoming message. > Checked by AVG - http://www.avg.com > Version: 8.0.138 / Virus Database: 270.5.12/1594 - Release Date: 8/5/2008 > 9:49 PM > > > ------------------------------ Message: 22 Date: Wed, 6 Aug 2008 07:33:31 -0500 From: "Drew Sally A." Subject: RE: [Histonet] Factor XIIIa antibody To: "Histonet" Message-ID: <3F328377AF4E23438E78234752652CE105D52772@uwhis-xchng7.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="US-ASCII" I'f you are at all interested in a polyclonal antibody, we have been happy with BioCare Medical's F13a antibody, and I would be happy to tell you more about it if you desire. Sally Ann Drew, MT (ASCP) sdrew@uwhealth.org IHC/ISH Lab DB1-223, Mail Code 3224 600 Highland Ave. Madison, WI 53792 Phone (608) 265-6596 Fax (608) 262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Young Kwun Sent: Tuesday, August 05, 2008 9:15 PM To: Histonet Subject: [Histonet] Factor XIIIa antibody Hello All, We've been using Factor XIII, A-subunit polyclonal antibody from Calbiochem (Cat.No.233498) on skin conditions successfully so far. However this antibody was discontinued few years ago and they are offering Factor XIII, S-subunit only. Although I haven't tried FXIII S-unit immunostaining on human skin, I tried two other monoclonal Factor XIIIa but the stains were not as good as our current one. I tried Neomaker and Novocastra's. Can anyone suggest the source of good FXIIIa please? Thank you. Young Kwun Senior Hospital Scientist Dept. of Anatomical Pathology Concord Hospital Concord NSW 2139 Australia Tel)61-2-9767-6075 Fax)61-2-9767-8427 kwuny@email.cs.nsw.gov.au _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 57, Issue 10 **************************************** ______________________________________________________________________________ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From gayle.callis <@t> bresnan.net Wed Aug 6 10:42:25 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Wed Aug 6 10:42:29 2008 Subject: [Histonet] Horse bones References: Message-ID: <000901c8f7db$069c8a80$6501a8c0@Sunney> How big are the pieces? It would be advisable to have longer processing schedule, rather than a routine schedule. This sounds like poor dehydration and poor paraffin infiltration. Also, if your samples are less than 3 mm thick, then you get the potato chip syndrome, where the bone is NOT supported well enough, plus trimming should be done in very thin increments (setting) with a SHARP blade. High profile blades work much better with harder bone but if the bone is perfectly and totally fixed before decalcification, tested, and processed properly, thin profile will work although not as nicely. You should do a decalcification endpoint test and there is a simple one that works quite nicely, a weight gain/weight loss. If the bone appears chalky or opaque, then the bone is either poorly decalcified, poorly processed or both. Longer infiltration in a harder paraffin i.e. Tissue Prep 2 ThermoScientific ala Fisher is excellent for bone and three changes minimum. The horse bone may be harder, depending on age of the animal, as compared to another species. Soaking only works IF the bone is well decalcified and processed. To avoid surface decalcification, endpoint test. I will be happy to send the protocol for that, but you need a balance that weighs in milligrams. Gayle M. Callis HTL/HT/MT(ASCP) ----- Original Message ----- From: To: Sent: Tuesday, August 05, 2008 6:07 PM Subject: [Histonet] Horse bones > We are trying to section pieces of horse femurs, fetlock and carpal > joints. > They have been decalcified with either HCl decal or formic acid decal. > Before routine processing the bones appear to be decalcified, but at > sectioning > they are very hard and brittle (even after surface decal) and chip out of > the > paraffin block. > Any suggestions would be helpful. A pathologist suggested a soap soaking > solution but could not remember the name of the soap. > > Help and thank you, > > R. Berger, HT > > > > > **************Looking for a car that's sporty, fun and fits in your > budget? > Read reviews on AOL Autos. > (http://autos.aol.com/cars-BMW-128-2008/expert-review?ncid=aolaut00050000000017 > ) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Wed Aug 6 10:47:14 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Wed Aug 6 10:47:19 2008 Subject: [Histonet] Re: crystal violet for amyloid Message-ID: Beth Allen is >>trying to find a procedure for staining fresh muscle tissue for crystal violet to demonstrate amyloid.<< I think there's a procedure in Lee Luna's 1968 AFIP manual. I haven't seen one of these stains in forty years. Mounting this stain is difficult - resin and glycerol jelly lose the metachromatic staining, and the stain must be mounted in sugar syrup (traditionally Karo (tm) syrup), and examined within a day of preparation. Photography is difficult. This is an obsolete amyloid stain. Congo red or one of the newer direct dyes would be preferable. Possibly the fluorescent stain Thioflavin T (probably also obsolete) would be a closer equivalent of crystal violet. Lotsa luck! Bob Richmond Samurai Pathologist Knoxville TN From Kimberly.Marshall <@t> ahss.org Wed Aug 6 11:07:32 2008 From: Kimberly.Marshall <@t> ahss.org (Marshall, Kimberly) Date: Wed Aug 6 11:07:41 2008 Subject: [Histonet] RE: horse Bones Message-ID: When softening hard stuff (bones and Hard skin from feet etc..) hair conditioner mixed with warm water will also soften tissue Kimberly ============================================================================== The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ============================================================================== From gwtiner <@t> duckworthpathology.com Wed Aug 6 11:11:08 2008 From: gwtiner <@t> duckworthpathology.com (Wayne Tiner) Date: Wed Aug 6 11:09:03 2008 Subject: [Histonet] Technical Consultant Message-ID: I am looking for recommendations concerning individuals qualified to assess the operations of a hospital-based histology laboratory operation. The interested party seeks an individual who can assess workflows and practices and who also has the experience and the technical skills to assess processing issues that impact the quality of the prepared materials. Please respond directly to my address below. Wayne Tiner Director, Reference Laboratory Services Duckworth Pathology Group, Inc. (901) 276-0288 (901) 581-0091 Mobile (901) 729-4335 Fax gwtiner@duckworthpathology.com P Save Paper - Do you really need to print this e-mail? The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. From gagnone <@t> KGH.KARI.NET Wed Aug 6 11:47:21 2008 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Wed Aug 6 11:47:28 2008 Subject: [Histonet] Her2 SP3 Message-ID: Hi Debbie, What staining platform are you using for your Her2, Ventana by any chance? Eric Gagnon MLT Histology Laboratory Kingston General Hospital, Kingston, Ontario, Canada From Maxim_71 <@t> mail.ru Wed Aug 6 12:17:28 2008 From: Maxim_71 <@t> mail.ru (Maxim_71@mail.ru) Date: Wed Aug 6 12:19:28 2008 Subject: [Histonet] Horse bones Message-ID: <1446850633.20080806211728@mail.ru> R. Berger: I was not be able to get good quality sections from bone specimens, until begann to use next things: 1- determination endpoint of decalcification 2- isopropanol as dehydratant 3- mineral oil as antemedium before parafiin infiltration. In my personal experience only these things had great improvement for quality of my bone sections. All rest things have had minimal importance for me. I must use manual processing, because have not any processor. Sincerely, Maxim Peshkov, Russia, Taganrog. -----Original message--- > Date: Tue, 5 Aug 2008 20:07:50 EDT > From: BERGERJL@aol.com > Subject: [Histonet] Horse bones > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset="US-ASCII" > > We are trying to section pieces of horse femurs, > fetlock and carpal joints. > They have been decalcified with either HCl decal or > formic acid decal. > Before routine processing the bones appear to be > decalcified, but at sectioning > they are very hard and brittle (even after surface > decal) and chip out of the > paraffin block. > Any suggestions would be helpful. A pathologist > suggested a soap soaking > solution but could not remember the name of the soap. > > Help and thank you, > > R. Berger, HT From sprice2003 <@t> gmail.com Wed Aug 6 12:24:45 2008 From: sprice2003 <@t> gmail.com (Sally Price) Date: Wed Aug 6 12:24:51 2008 Subject: [Histonet] protein block Message-ID: Gene: What you've decribed is not, in my expericence, that uncommon. It all depends on the blocking agent your using -- some protein blocks are made from casein or digested immunoglobulin (e.g Fab2 fargments), some are made with only animal serum, and others are combinations of these materials -- If the reagent you're using is made with animal serum, for example, the backgroud could be caused by the blocking agent sticking to endogenous immunoglobulins with the specimen. At least this is one possibility, and the only way to confirm/resolve this issue is to try a differnt type of protein blocker. Cheers, Sally ------------------------------ Message: 16 Date: Tue, 05 Aug 2008 05:49:59 -0400 From: njoydobro@aol.com Subject: [Histonet] protein block To: Histonet@lists.utsouthwestern.edu Good Morning everyone, ???? We are currently working up a new antibody and are trying it with and without protein block.? We are getting some unexpected results and I would like to see if anyone has seen similar outcomes.? We are getting less background without the protein block and the staining appears "crisper".? Anyone experienced this? Thanks, Gene From dawn_cowie <@t> yahoo.com Wed Aug 6 13:29:23 2008 From: dawn_cowie <@t> yahoo.com (Dawn Cowie) Date: Wed Aug 6 13:30:30 2008 Subject: [Histonet] surveillance cameras in the lab In-Reply-To: <582736990808050623v3dac9e92xaec4f1a67e3a8b83@mail.gmail.com> Message-ID: <430954.54820.qm@web45013.mail.sp1.yahoo.com> I didn't read the initial posts on this topic so I don't know why the lab has installed cameras. I will say that if it is because of problem employees, you get back to the fact of having poor management and supervision in place. ? Dawn Cowie, HT --- On Tue, 8/5/08, Amos Brooks wrote: From: Amos Brooks Subject: [Histonet] surveillance cameras in the lab To: pruegg@ihctech.net, "histonet@lists.utsouthwestern.edu" Date: Tuesday, August 5, 2008, 9:23 AM Patsy, I have to disagree with you there. I understand the need for security, but there is a certain amount of trust lost when one makes the decision to monitor employees without even informing them as to why. (Weather or not they are legally bound to do so.) You can accomplish the same thing by letting employees know that there is a problem (or a potential problem) and having them beware and observant. As a last resort when they find the problem still presists using stromger methods becomes warranted. Once you give up this personal liberty and allow someone to watch over your shoulder all the time you loose the ability to make the right choices as there are no choices to make. I would rather live in a society of people that want to do the right thing than one of people that are forced to. At that point we might as well allow those that would do us harm to make all the decisions for us and abandon any form of personal freedom we may have. Ben Franklin published in Poor Richard's Almanac the famous quote *"Those who would give up essential liberty to purchase a little temporary safety, deserve neither liberty nor safety."* It is important to temper our fears with a sense of who we are lest we devolve into a military state. Thanks, Amos Brooks Message: 19 Date: Mon, 4 Aug 2008 10:46:26 -0600 From: "Patsy Ruegg" Subject: RE: [Histonet] surveillance cameras in the lab To: "'Cheri Miller'" , , Message-ID: Content-Type: text/plain; charset="us-ascii" Come on. In these times of terror concerns I am not sure I would work in a place where I did not feel secure and the use of these devices help in that matter. We built a brand new University of Colorado Health Sciences Center and there are cameras all over the place as well as lock down. If you do not have an access card you cannot get into the labs. This was a pain at first but with all the crazy's we have to worry about out there it now makes me feel better. I just read in the paper this morning about a researcher whose house was bombed by Peta types for doing animal research, and we have had all sorts of disturbances over the years with precious research animals being released, protests, etc. Patsy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amber.mckenzie <@t> gastrodocs.net Wed Aug 6 14:28:35 2008 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Wed Aug 6 14:28:43 2008 Subject: [Histonet] NSH class Message-ID: <03C921A1EAF7F541B16543F6EC6A4B3701D3B5C8@giamail2.Gia.com> I am not able to attend the NSH convention this year, and I was looking at the Program guide...on Sunday from 12:30-4pm is a class "How to Start an accredited Histotech Program" by Peggy Wenk...anyone know how/if I could get the information from that class? Amber McKenzie, B.S., HT (ASCP) 1405 N. State St., Suite 400 Jackson, MS 39202 (ph) 601-863-0388 (fax) 601-326-3532 From walterj <@t> HanoverHospital.org Wed Aug 6 14:30:13 2008 From: walterj <@t> HanoverHospital.org (Walter, Janelle) Date: Wed Aug 6 14:30:19 2008 Subject: [Histonet] Meditech Help Message-ID: Is there anyone who I can talk to who has had experience setting up (or even using) the Meditech PTH module for Histology & Cytology? We are currently using CoPath, but have been 'sweet-talked' into changing to Meditech. Any help would be greatly appreciated. Thanks, Janelle Janelle D. Walter, BS MT(ASCP) Clinical Analyst, MIS Hanover Hospital 300 Highland Avenue Hanover, PA 17331 717-646-6899 fax:717-633-3521 walterj@hanoverhospital.org CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please respond immediately by returning this e-mail to the sender and destroying all copies of this communication including any attachments. From ccrowder <@t> vetmed.lsu.edu Wed Aug 6 16:45:16 2008 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Wed Aug 6 16:50:56 2008 Subject: [Histonet] Poster Judges Needed Message-ID: Due to "circumstances beyond our control", 2 of the judged for the NSH Poster Committee will be unable to attend the Symposium/Convention in Pittsburgh. So, as chair of the committee, I am looking for 2 volunteers to judge posters. Qualifications include NSH membership and attendance at the Pittsburgh meeting and knowledge of clinical, research and educational histology. You don't have to have worked in all areas to be familiar with work done by others. A working knowledge of publication methods is helpful. The "job" requires that you talk to the presenters Sunday between 10:30 and 12:30, review the posters and judge them in specific areas sometime between Sunday and Monday afternoon, compile your results (that doesn't take long) and meet with the other members Monday afternoon. For all this you do not get paid, stay essentially anonymous and blow you mind from reviewing outstanding work by our members. If you are interested, please contact me as soon as possible by e-mail or phone. As someone said, it's the hardest job you'll ever love. Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 From joelleweaver <@t> hotmail.com Wed Aug 6 17:44:32 2008 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Wed Aug 6 17:44:41 2008 Subject: [Histonet] HT schools In-Reply-To: References: Message-ID: Those of us who particpate and work in Histology education, do as much as we can! I know that I put in about 20-30 hours a week plus my FT histology job trying to promote histology and being a histotech. For the most part, I get ignored. But just takes MORE people. LOTS more people to particpate. JMW > From: jdhisto@yahoo.com> Date: Tue, 5 Aug 2008 04:04:13 -0500> To: Histonet@lists.utsouthwestern.edu> CC: > Subject: [Histonet] HT schools> > Hello,> I totally agree with Jennifer Mcdonalds comment. Yes it is all about marketing. People, students , most individuals do not know about Histology. They barely know what the word means, they usually think that its some kind of study of history (go figure). > > I have meet so many students repeatedly (biology, medical technology, chemistry > majors) who love the idea of a career in histotechnology. Especially after finding out the many options there are besides being in a high volume lab cutting 200-300 blocks per day if not more. > > If people dont know...we dont grow. > Till then..> > Sincerely,> JDhisto> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Your PC, mobile phone, and online services work together like never before. http://clk.atdmt.com/MRT/go/108587394/direct/01/ From BORISA <@t> trinity-health.org Thu Aug 7 08:18:32 2008 From: BORISA <@t> trinity-health.org (Anthony Boris) Date: Thu Aug 7 08:18:49 2008 Subject: [Histonet] Job Opening References: <489ABCF9.7813.00DE.0@trinity-health.org> Message-ID: <489ABDE8.7813.00DE.0@trinity-health.org> St Joseph Mercy Oakland in Pontiac, Michigan has a full time opening for a HT or HTL (ASCP). Registry eligible and new grads are encouraged to apply. It is a day shift position, Monday-Friday. Candidate must be proficient in routine histology skills. Special stain and Immunohistochemistry experience is desired. The wage scale is highly aggressive and excellent benefits are offered. St Joes even has a free concierge service that will run your errands while you are at work! Please apply online at http://sjmoweb.trinity-health.org/. or call 248-858-6231 for more info Thanks Tony Boris From mroark <@t> sfmc.net Thu Aug 7 08:27:26 2008 From: mroark <@t> sfmc.net (Matthew Roark) Date: Thu Aug 7 08:28:02 2008 Subject: [Histonet] Coverslipper Message-ID: <489AB1EE02000011000665B6@email_gateway.sfmc.net> We are currently in the market for a new coverslipper. What are you using and/or what would everyone recommend? We currently have an old Leica glass coverslipper but would be open to try something new. Thanks! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 From rjbuesa <@t> yahoo.com Thu Aug 7 08:44:18 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Aug 7 08:44:21 2008 Subject: [Histonet] Coverslipper In-Reply-To: <489AB1EE02000011000665B6@email_gateway.sfmc.net> Message-ID: <274640.66247.qm@web65710.mail.ac4.yahoo.com> Try the Sakura film, or if you?prefer glass, the Sakura glass coverslipper. Ren? J. --- On Thu, 8/7/08, Matthew Roark wrote: From: Matthew Roark Subject: [Histonet] Coverslipper To: Histonet@lists.utsouthwestern.edu Date: Thursday, August 7, 2008, 9:27 AM We are currently in the market for a new coverslipper. What are you using and/or what would everyone recommend? We currently have an old Leica glass coverslipper but would be open to try something new. Thanks! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Masterson_John <@t> Allergan.com Thu Aug 7 08:33:43 2008 From: Masterson_John <@t> Allergan.com (Masterson_John) Date: Thu Aug 7 08:45:20 2008 Subject: [Histonet] GMA 1 micron section staining In-Reply-To: <489ABDE8.7813.00DE.0@trinity-health.org> References: <489ABCF9.7813.00DE.0@trinity-health.org> <489ABDE8.7813.00DE.0@trinity-health.org> Message-ID: <0C58C4F16F0B67448318A38041CADE4B01594F26@IRMAIL133.irvine.allergan.com> Hello Histonetters, I need to stain some 1 micron sections of GMA embedded tissue for H&E. Does anyone have experience they can share? Thanks in advance. John From jqb7 <@t> cdc.gov Thu Aug 7 08:48:27 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu Aug 7 08:48:46 2008 Subject: [Histonet] Coverslipper In-Reply-To: <489AB1EE02000011000665B6@email_gateway.sfmc.net> References: <489AB1EE02000011000665B6@email_gateway.sfmc.net> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70208BF30@LTA3VS011.ees.hhs.gov> We have one of the newer Leica's and have just recently ordered one of Sakura's as well. We have not yet tried the Sakura brand. We have to have glass and have been very pleased with the Leica. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Roark Sent: Thursday, August 07, 2008 9:27 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslipper We are currently in the market for a new coverslipper. What are you using and/or what would everyone recommend? We currently have an old Leica glass coverslipper but would be open to try something new. Thanks! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Thu Aug 7 09:01:59 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Aug 7 09:02:03 2008 Subject: [Histonet] Coverslipper In-Reply-To: <489AB1EE02000011000665B6@email_gateway.sfmc.net> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A38CB@IS-E2K3.grhs.net> I love our Sakura film. Have had no problems with it in over 10 years, just general maintenance. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Roark Sent: Thursday, August 07, 2008 8:27 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslipper We are currently in the market for a new coverslipper. What are you using and/or what would everyone recommend? We currently have an old Leica glass coverslipper but would be open to try something new. Thanks! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Thu Aug 7 09:12:07 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Aug 7 09:12:28 2008 Subject: [Histonet] Coverslipper In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A38CB@IS-E2K3.grhs.net> Message-ID: We have been using the Sakura film coverslipper for over a year - slides can be put into slide racks within minutes after coverslipping. Great when you have to turn slides around or mail them out quickly. Jackie O' "Mike Pence" Sent by: histonet-bounces@lists.utsouthwestern.edu 08/07/2008 09:01 AM To "Matthew Roark" , cc Subject RE: [Histonet] Coverslipper I love our Sakura film. Have had no problems with it in over 10 years, just general maintenance. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Roark Sent: Thursday, August 07, 2008 8:27 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslipper We are currently in the market for a new coverslipper. What are you using and/or what would everyone recommend? We currently have an old Leica glass coverslipper but would be open to try something new. Thanks! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Thu Aug 7 10:12:31 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Thu Aug 7 10:12:37 2008 Subject: [Histonet] GMA 1 micron section staining References: <489ABCF9.7813.00DE.0@trinity-health.org><489ABDE8.7813.00DE.0@trinity-health.org> <0C58C4F16F0B67448318A38041CADE4B01594F26@IRMAIL133.irvine.allergan.com> Message-ID: <001d01c8f8a0$035fb980$6501a8c0@Sunney> Do not rinse but put dry sections directly to Gill 3 hematoxylin 10 minutes. Time can vary. Rinse 3 X with distilled water Go directly to bluing 1 min Scotts Tap water substitute Rinse with distilled water Air dry sections Eosin/phloxine for 2 to 5 minutes time will vary. Rinse very quickly through 2 changes of 95% ethanol only and air dry quickly. I liked to use compressed or forced air, a fan works nicely. Coverslip with permanent mounting media over dry section. Be aware that 1 um is really thin and will not look like a regular H&E on a thicker section. There just isn't much tissue in a 1 um thick section. I had pathologists complain, but when this was explained, they understood what was going on since they were used to looking at 5 um thick sections. Alcohols during dehyration will cause sections to release or bulge from slide. Xylene can create problems too so we always mounted a coverglass over a dry section. You can thin the mounting media a bit just in case it doesn't flow well. If you have a bit of funky plastic fold over of plastic, that can be scraped away with a teflon coated razor blade, to not have extra thickness in a bad place during coverslipping. We did not use Harris hematoxylin for GMA sections, and this method basically is what Polysciences put out years ago when GMA first came out, and it worked very well for us over the years. Good luck on staining Gayle M. Callis HTL/HT/MT(ASCP) ----- Original Message ----- From: "Masterson_John" To: "Anthony Boris" ; Sent: Thursday, August 07, 2008 7:33 AM Subject: [Histonet] GMA 1 micron section staining Hello Histonetters, I need to stain some 1 micron sections of GMA embedded tissue for H&E. Does anyone have experience they can share? Thanks in advance. John _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amartinez <@t> carisdx.com Thu Aug 7 11:20:01 2008 From: amartinez <@t> carisdx.com (Martinez, Angela) Date: Thu Aug 7 11:22:27 2008 Subject: [Histonet] Job Opening In Ft. Worth Texas Message-ID: <9B8A3AC772C7F64680392A7CB8FBFB0F05549E1D@s-irv-ex301.PathologyPartners.intranet> Great opportunity for Histotechnician in brand new laboratory! Gastrointestinal Associates of North Texas in Ft Worth , is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and meet CLIA-88 regulations to perform gross dissection. Prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. The position offers competitive salary, medical insurance, retirement plan, and vacation /sick leave. Interested applicants should e-mail resumes to Meredith Hale at mhale@carisdx.com . Meredith Hale HT (ASCP) Technical Director Caris Diagnostics 8400 Esters Blvd. Ste. 190 Irving, Texas 75063 From kmerriam2003 <@t> yahoo.com Thu Aug 7 11:36:02 2008 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Thu Aug 7 11:36:06 2008 Subject: [Histonet] cryostat block holders Message-ID: <611208.37592.qm@web50305.mail.re2.yahoo.com> Hi everyone, I want to order some disposable cryostat block holders (the round things that you attach your block to).? I?don't know what they are officially called, so I am having trouble searching for them.??Does anyone know what they are called and if anyone sells?plastic ones? Thanks, Kim ?Kim Merriam, MA, HT(ASCP) Cambridge, MA From maa8 <@t> cornell.edu Thu Aug 7 11:43:14 2008 From: maa8 <@t> cornell.edu (Mary Ascenzi) Date: Thu Aug 7 11:44:51 2008 Subject: [Histonet] camera for microscope Message-ID: <6.2.1.2.2.20080807123426.01e0a900@postoffice6.mail.cornell.edu> Hello All I have an old Olympus microscope and am looking for a digital camera for photomics of slides. I know that there are cameras designed specifically for microscopes, but am wondering if anyone has used a digital SLR with an adapter. What is the photo quality, ie sharpness, color fidelity ? What software do you use ? Is Photoshop adequate? thanks, Mary From cbass <@t> wfubmc.edu Thu Aug 7 12:12:50 2008 From: cbass <@t> wfubmc.edu (Caroline Bass) Date: Thu Aug 7 12:12:56 2008 Subject: [Histonet] Microglia antibody Message-ID: Hello Everyone, I?m looking for an antibody to stain microglia in rat brain sections. These will be perfused with formalin and sectioned at about 60 microns. I?ll probably start with an ABC kit, but would also like the ability to use the antibody for immunofluorescence. Are there any recommendations? Thanks, Caroline Bass From liz <@t> premierlab.com Thu Aug 7 12:24:08 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Aug 7 12:24:26 2008 Subject: [Histonet] Microglia antibody In-Reply-To: References: Message-ID: Caroline I believe you can use ED-1 or OX42 or both. Both work in FFPE tissue samples. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Caroline Bass Sent: Thursday, August 07, 2008 11:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microglia antibody Hello Everyone, I?m looking for an antibody to stain microglia in rat brain sections. These will be perfused with formalin and sectioned at about 60 microns. I?ll probably start with an ABC kit, but would also like the ability to use the antibody for immunofluorescence. Are there any recommendations? Thanks, Caroline Bass _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rchiovetti <@t> yahoo.com Thu Aug 7 12:30:06 2008 From: rchiovetti <@t> yahoo.com (Robert Chiovetti) Date: Thu Aug 7 12:30:10 2008 Subject: [Histonet] camera for microscope Message-ID: <413217.42314.qm@web58902.mail.re1.yahoo.com> Mary, For general use and simple documentation, I've had good luck with both digital SLR's and some of the "prosumer" handheld digital cameras (such as the Canon PowerShot S3 and S5). Good resoution, pretty faithful color reproduction, no significant problems. Some of these cameras have TWAIN drivers, so you can even operate the camera from within Photoshop if you choose. With the "prosumer" cameras, you'll have to play with the zoom settings to get a full field of view, but that's a minor concern. There are a couple of vendors who specialize in adapters for these cameras; you can mate the camera to a scope's trinocular (photo) port if it's so equipped, but even in the absence of a photo port, you can get an adapter for the eyepiece tube...just remove an eyepiece, and slip the camera/adapter in place. Most of these types of cameras will play digital games with the original image when you take a picture, meaning the image is interpolated...that's probably not the best situation if you plan on doing any kind of image analysis, statistics, measurement, etc. on the image. But for basic documentation, a DSLR or prosumer camera works great! My 2 cents' worth... Contact me off-list if you need more info. Bob Robert (Bob) Chiovetti, Ph.D. Southwest Precision Instruments www.swpinet.com See What's New on Our Website! Arizona's Microscopy Resource 132 North Elster Drive Tucson, AZ 85710-3212 Tel./Fax 520-546-4986 Member, Arizona Small Business Association (www.asba.com) ----- Original Message ---- From: Mary Ascenzi To: histonet@lists.utsouthwestern.edu Sent: Thursday, August 7, 2008 11:43:14 AM Subject: [Histonet] camera for microscope Hello All I have an old Olympus microscope and am looking for a digital camera for photomics of slides. I know that there are cameras designed specifically for microscopes, but am wondering if anyone has used a digital SLR with an adapter. What is the photo quality, ie sharpness, color fidelity ? What software do you use ? Is Photoshop adequate? thanks, Mary _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cbass <@t> wfubmc.edu Thu Aug 7 12:31:44 2008 From: cbass <@t> wfubmc.edu (Caroline Bass) Date: Thu Aug 7 12:31:50 2008 Subject: [Histonet] Microglia antibody In-Reply-To: <489B30E3.50203@umdnj.edu> Message-ID: Thanks for the suggestions. I've used F4/80 in the past, I think this one was from serotec, but I used it in mouse sections. Does anyone have experience with this antigen or antibody? Thanks! On 8/7/08 1:29 PM, "Geoff McAuliffe" wrote: > Hi Caroline: > > Both ED-1 and OX42 stain rat brain microglia, but different > populations. I will try to dig up a reference for you. > > Geoff > > Caroline Bass wrote: >> Hello Everyone, >> >> I?m looking for an antibody to stain microglia in rat brain sections. These >> will be perfused with formalin and sectioned at about 60 microns. I?ll >> probably start with an ABC kit, but would also like the ability to use the >> antibody for immunofluorescence. Are there any recommendations? >> >> Thanks, >> >> Caroline Bass >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > From mcauliff <@t> umdnj.edu Thu Aug 7 12:29:07 2008 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Aug 7 13:38:58 2008 Subject: [Histonet] Microglia antibody In-Reply-To: References: Message-ID: <489B30E3.50203@umdnj.edu> Hi Caroline: Both ED-1 and OX42 stain rat brain microglia, but different populations. I will try to dig up a reference for you. Geoff Caroline Bass wrote: > Hello Everyone, > > I?m looking for an antibody to stain microglia in rat brain sections. These > will be perfused with formalin and sectioned at about 60 microns. I?ll > probably start with an ABC kit, but would also like the ability to use the > antibody for immunofluorescence. Are there any recommendations? > > Thanks, > > Caroline Bass > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From Jack.Schug <@t> ridgeviewmedical.org Thu Aug 7 13:43:57 2008 From: Jack.Schug <@t> ridgeviewmedical.org (Schug, Jack) Date: Thu Aug 7 13:42:02 2008 Subject: [Histonet] PowerPath Message-ID: <8EE20F4C24C068458E01ABCE51B85CF30655B6EF@rmcexch01.ridgeview.local> PowerPath users: Does anyone have a procedure in the event that PowerPath crashes? Are you willing to share. Thanks, Jack Ridgeview Medical Center Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. From plucas <@t> biopath.org Thu Aug 7 13:51:45 2008 From: plucas <@t> biopath.org (Paula Lucas) Date: Thu Aug 7 13:51:48 2008 Subject: [Histonet] Job Opening Message-ID: <20080807185145.1CAE127F6@courageux.cnchost.com> Attn: Immediate job opening for an experienced Histotech. We are located in Fountain Valley, California. Please fax resume to: Bio-Path Medical Group c/o Paula Lucas 714-755-2984 From nsnwl <@t> neuro.hfh.edu Thu Aug 7 14:10:54 2008 From: nsnwl <@t> neuro.hfh.edu (Nancy Lemke) Date: Thu Aug 7 14:13:31 2008 Subject: [Histonet] Microglia antibody Message-ID: <7f448b407857b93194581ca8247e77f0@neuro.hfh.edu> Hi Caroline, I use Serotec CD68 (MCA341R) with wonderful results. I retrieve in citrate buffer pH 6.0 on a hotplate (boil 10 and cool 20), and dilute the antibody to 1:200, incubate for 30 minutes and use Biocare 4+ avidin-biotinHRP detection and DAB. The stain is very clean and reliable. Nancy Lemke Nancy Lemke Research Coordinator Hermelin Brain Tumor Center Henry Ford Hospital Detroit -----Original message----- From: "Liz Chlipala" liz@premierlab.com Date: Thu, 07 Aug 2008 13:25:09 -0400 To: "Caroline Bass" cbass@wfubmc.edu Subject: RE: [Histonet] Microglia antibody > Caroline > > I believe you can use ED-1 or OX42 or both. Both work in FFPE tissue > samples. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Manager > Premier Laboratory, LLC > P.O. Box 18592 > Boulder, CO 80308 > phone (303) 682-3949 > fax (303) 682-9060 > liz@premierlab.com > www.premierlab.com > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive > Unit E > Longmont, CO 80504 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Caroline > Bass > Sent: Thursday, August 07, 2008 11:13 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Microglia antibody > > Hello Everyone, > > I¹m looking for an antibody to stain microglia in rat brain sections. > These will be perfused with formalin and sectioned at about 60 microns. > I¹ll probably start with an ABC kit, but would also like the ability > to use the antibody for immunofluorescence. Are there any > recommendations? > > Thanks, > > Caroline Bass > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ============================================================================== CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by reply email, delete the email from your computer system and shred any paper copies. Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. See our Privacy Policy and Henry Ford My Health at www.henryford.com for more detailed information. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== From liz <@t> premierlab.com Thu Aug 7 14:32:53 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Aug 7 14:33:03 2008 Subject: [Histonet] Microglia antibody In-Reply-To: References: <489B30E3.50203@umdnj.edu> Message-ID: F4/80 is used in mouse tissue, it does not work in rat tissue. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Caroline Bass Sent: Thursday, August 07, 2008 11:32 AM To: Geoff McAuliffe Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Microglia antibody Thanks for the suggestions. I've used F4/80 in the past, I think this one was from serotec, but I used it in mouse sections. Does anyone have experience with this antigen or antibody? Thanks! On 8/7/08 1:29 PM, "Geoff McAuliffe" wrote: > Hi Caroline: > > Both ED-1 and OX42 stain rat brain microglia, but different > populations. I will try to dig up a reference for you. > > Geoff > > Caroline Bass wrote: >> Hello Everyone, >> >> I?m looking for an antibody to stain microglia in rat brain sections. >> These will be perfused with formalin and sectioned at about 60 >> microns. I?ll probably start with an ABC kit, but would also like >> the ability to use the antibody for immunofluorescence. Are there any recommendations? >> >> Thanks, >> >> Caroline Bass >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Thu Aug 7 14:37:26 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Thu Aug 7 14:37:29 2008 Subject: [Histonet] Microglia antibody References: Message-ID: <001801c8f8c5$055e6fe0$6501a8c0@Sunney> It is specific antibody for mouse and not rat. People have used it with FFPE tissue. This has been put on Histonet several times, including the retrieval you need to use. We only use it with cryostat sections, our special acetone alcohol fixation. For fluoescence work, we only use cryostat sections with this fixative to avoid aldehyde induced autofluorescence. If one uses it for IFA, then it would be wise to remove free aldehydes to reduce the autofluorescence problem. Gayle M. Callis HTL/HT/MT(ASCP) ----- Original Message ----- From: "Caroline Bass" To: "Geoff McAuliffe" Cc: Sent: Thursday, August 07, 2008 11:31 AM Subject: Re: [Histonet] Microglia antibody Thanks for the suggestions. I've used F4/80 in the past, I think this one was from serotec, but I used it in mouse sections. Does anyone have experience with this antigen or antibody? Thanks! On 8/7/08 1:29 PM, "Geoff McAuliffe" wrote: > Hi Caroline: > > Both ED-1 and OX42 stain rat brain microglia, but different > populations. I will try to dig up a reference for you. > > Geoff > > Caroline Bass wrote: >> Hello Everyone, >> >> I?m looking for an antibody to stain microglia in rat brain sections. >> These >> will be perfused with formalin and sectioned at about 60 microns. I?ll >> probably start with an ABC kit, but would also like the ability to use >> the >> antibody for immunofluorescence. Are there any recommendations? >> >> Thanks, >> >> Caroline Bass >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Aug 7 15:26:31 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Aug 7 15:26:35 2008 Subject: [Histonet] cryostat block holders In-Reply-To: <611208.37592.qm@web50305.mail.re2.yahoo.com> Message-ID: <586052.27945.qm@web65714.mail.ac4.yahoo.com> Chucks. Ren? J. --- On Thu, 8/7/08, Kim Merriam wrote: From: Kim Merriam Subject: [Histonet] cryostat block holders To: "Histonet" Date: Thursday, August 7, 2008, 12:36 PM Hi everyone, I want to order some disposable cryostat block holders (the round things that you attach your block to).? I?don't know what they are officially called, so I am having trouble searching for them.??Does anyone know what they are called and if anyone sells?plastic ones? Thanks, Kim ?Kim Merriam, MA, HT(ASCP) Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Pemberton <@t> bmcjax.com Thu Aug 7 16:29:32 2008 From: Susan.Pemberton <@t> bmcjax.com (Pemberton, Susan) Date: Thu Aug 7 16:29:39 2008 Subject: [Histonet] RE: Coverslipper In-Reply-To: <200808071702.m77H2IdA024723@bhagent1.bmcjax.com> Message-ID: <4E59E1E7B5A80F448F47424C8A2FDEDB02295CA4@BHEXCHVS02.BH.LOCAL> We have a Leica stainer/coverslipper - it's two years old. We like it, but we are having trouble with bubbles and can't seem to adjust them out. Any suggestions? Susan Pemberton MS,MT(ASCP)SM Laboratory Administrative Director Baptist Health 800 Prudential Drive Jacksonville, FL 32207 904.202.2016 Fax:904.202.2795 susan.pemberton@bmcjax.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, August 07, 2008 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 57, Issue 13 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Horse bones (Maxim_71@mail.ru) 2. RE: protein block (Sally Price) 3. Re: surveillance cameras in the lab (Dawn Cowie) 4. NSH class (Amber McKenzie) 5. Meditech Help (Walter, Janelle) 6. Poster Judges Needed (Cheryl Crowder) 7. RE: HT schools (joelle weaver) 8. Job Opening (Anthony Boris) 9. Coverslipper (Matthew Roark) 10. Re: Coverslipper (Rene J Buesa) 11. GMA 1 micron section staining (Masterson_John) 12. RE: Coverslipper (Bartlett, Jeanine (CDC/CCID/NCZVED)) 13. RE: Coverslipper (Mike Pence) 14. RE: Coverslipper (Jackie M O'Connor) 15. Re: GMA 1 micron section staining (Gayle Callis) 16. Job Opening In Ft. Worth Texas (Martinez, Angela) 17. cryostat block holders (Kim Merriam) 18. camera for microscope (Mary Ascenzi) ---------------------------------------------------------------------- Message: 1 Date: Wed, 6 Aug 2008 21:17:28 +0400 From: Maxim_71@mail.ru Subject: Re: [Histonet] Horse bones To: BERGERJL@aol.com Cc: histonet@lists.utsouthwestern.edu Message-ID: <1446850633.20080806211728@mail.ru> Content-Type: text/plain; charset=us-ascii R. Berger: I was not be able to get good quality sections from bone specimens, until begann to use next things: 1- determination endpoint of decalcification 2- isopropanol as dehydratant 3- mineral oil as antemedium before parafiin infiltration. In my personal experience only these things had great improvement for quality of my bone sections. All rest things have had minimal importance for me. I must use manual processing, because have not any processor. Sincerely, Maxim Peshkov, Russia, Taganrog. -----Original message--- > Date: Tue, 5 Aug 2008 20:07:50 EDT > From: BERGERJL@aol.com > Subject: [Histonet] Horse bones > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset="US-ASCII" > > We are trying to section pieces of horse femurs, > fetlock and carpal joints. > They have been decalcified with either HCl decal or > formic acid decal. > Before routine processing the bones appear to be > decalcified, but at sectioning > they are very hard and brittle (even after surface > decal) and chip out of the > paraffin block. > Any suggestions would be helpful. A pathologist > suggested a soap soaking > solution but could not remember the name of the soap. > > Help and thank you, > > R. Berger, HT ------------------------------ Message: 2 Date: Wed, 6 Aug 2008 13:24:45 -0400 From: "Sally Price" Subject: RE: [Histonet] protein block To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Gene: What you've decribed is not, in my expericence, that uncommon. It all depends on the blocking agent your using -- some protein blocks are made from casein or digested immunoglobulin (e.g Fab2 fargments), some are made with only animal serum, and others are combinations of these materials -- If the reagent you're using is made with animal serum, for example, the backgroud could be caused by the blocking agent sticking to endogenous immunoglobulins with the specimen. At least this is one possibility, and the only way to confirm/resolve this issue is to try a differnt type of protein blocker. Cheers, Sally ------------------------------ Message: 16 Date: Tue, 05 Aug 2008 05:49:59 -0400 From: njoydobro@aol.com Subject: [Histonet] protein block To: Histonet@lists.utsouthwestern.edu Good Morning everyone, ???? We are currently working up a new antibody and are trying it with and without protein block.? We are getting some unexpected results and I would like to see if anyone has seen similar outcomes.? We are getting less background without the protein block and the staining appears "crisper".? Anyone experienced this? Thanks, Gene ------------------------------ Message: 3 Date: Wed, 6 Aug 2008 11:29:23 -0700 (PDT) From: Dawn Cowie Subject: Re: [Histonet] surveillance cameras in the lab To: pruegg@ihctech.net, "histonet@lists.utsouthwestern.edu" , Amos Brooks Message-ID: <430954.54820.qm@web45013.mail.sp1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I didn't read the initial posts on this topic so I don't know why the lab has installed cameras. I will say that if it is because of problem employees, you get back to the fact of having poor management and supervision in place. ? Dawn Cowie, HT --- On Tue, 8/5/08, Amos Brooks wrote: From: Amos Brooks Subject: [Histonet] surveillance cameras in the lab To: pruegg@ihctech.net, "histonet@lists.utsouthwestern.edu" Date: Tuesday, August 5, 2008, 9:23 AM Patsy, I have to disagree with you there. I understand the need for security, but there is a certain amount of trust lost when one makes the decision to monitor employees without even informing them as to why. (Weather or not they are legally bound to do so.) You can accomplish the same thing by letting employees know that there is a problem (or a potential problem) and having them beware and observant. As a last resort when they find the problem still presists using stromger methods becomes warranted. Once you give up this personal liberty and allow someone to watch over your shoulder all the time you loose the ability to make the right choices as there are no choices to make. I would rather live in a society of people that want to do the right thing than one of people that are forced to. At that point we might as well allow those that would do us harm to make all the decisions for us and abandon any form of personal freedom we may have. Ben Franklin published in Poor Richard's Almanac the famous quote *"Those who would give up essential liberty to purchase a little temporary safety, deserve neither liberty nor safety."* It is important to temper our fears with a sense of who we are lest we devolve into a military state. Thanks, Amos Brooks Message: 19 Date: Mon, 4 Aug 2008 10:46:26 -0600 From: "Patsy Ruegg" Subject: RE: [Histonet] surveillance cameras in the lab To: "'Cheri Miller'" , , Message-ID: Content-Type: text/plain; charset="us-ascii" Come on. In these times of terror concerns I am not sure I would work in a place where I did not feel secure and the use of these devices help in that matter. We built a brand new University of Colorado Health Sciences Center and there are cameras all over the place as well as lock down. If you do not have an access card you cannot get into the labs. This was a pain at first but with all the crazy's we have to worry about out there it now makes me feel better. I just read in the paper this morning about a researcher whose house was bombed by Peta types for doing animal research, and we have had all sorts of disturbances over the years with precious research animals being released, protests, etc. Patsy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Wed, 6 Aug 2008 14:28:35 -0500 From: "Amber McKenzie" Subject: [Histonet] NSH class To: Message-ID: <03C921A1EAF7F541B16543F6EC6A4B3701D3B5C8@giamail2.Gia.com> Content-Type: text/plain; charset="US-ASCII" I am not able to attend the NSH convention this year, and I was looking at the Program guide...on Sunday from 12:30-4pm is a class "How to Start an accredited Histotech Program" by Peggy Wenk...anyone know how/if I could get the information from that class? Amber McKenzie, B.S., HT (ASCP) 1405 N. State St., Suite 400 Jackson, MS 39202 (ph) 601-863-0388 (fax) 601-326-3532 ------------------------------ Message: 5 Date: Wed, 6 Aug 2008 15:30:13 -0400 From: "Walter, Janelle" Subject: [Histonet] Meditech Help To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Is there anyone who I can talk to who has had experience setting up (or even using) the Meditech PTH module for Histology & Cytology? We are currently using CoPath, but have been 'sweet-talked' into changing to Meditech. Any help would be greatly appreciated. Thanks, Janelle Janelle D. Walter, BS MT(ASCP) Clinical Analyst, MIS Hanover Hospital 300 Highland Avenue Hanover, PA 17331 717-646-6899 fax:717-633-3521 walterj@hanoverhospital.org CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please respond immediately by returning this e-mail to the sender and destroying all copies of this communication including any attachments. ------------------------------ Message: 6 Date: Wed, 06 Aug 2008 16:45:16 -0500 From: "Cheryl Crowder" Subject: [Histonet] Poster Judges Needed To: "Histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" Due to "circumstances beyond our control", 2 of the judged for the NSH Poster Committee will be unable to attend the Symposium/Convention in Pittsburgh. So, as chair of the committee, I am looking for 2 volunteers to judge posters. Qualifications include NSH membership and attendance at the Pittsburgh meeting and knowledge of clinical, research and educational histology. You don't have to have worked in all areas to be familiar with work done by others. A working knowledge of publication methods is helpful. The "job" requires that you talk to the presenters Sunday between 10:30 and 12:30, review the posters and judge them in specific areas sometime between Sunday and Monday afternoon, compile your results (that doesn't take long) and meet with the other members Monday afternoon. For all this you do not get paid, stay essentially anonymous and blow you mind from reviewing outstanding work by our members. If you are interested, please contact me as soon as possible by e-mail or phone. As someone said, it's the hardest job you'll ever love. Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 ------------------------------ Message: 7 Date: Wed, 6 Aug 2008 22:44:32 +0000 From: joelle weaver Subject: RE: [Histonet] HT schools To: , JD Message-ID: Content-Type: text/plain; charset="iso-8859-1" Those of us who particpate and work in Histology education, do as much as we can! I know that I put in about 20-30 hours a week plus my FT histology job trying to promote histology and being a histotech. For the most part, I get ignored. But just takes MORE people. LOTS more people to particpate. JMW > From: jdhisto@yahoo.com> Date: Tue, 5 Aug 2008 04:04:13 -0500> To: Histonet@lists.utsouthwestern.edu> CC: > Subject: [Histonet] HT schools> > Hello,> I totally agree with Jennifer Mcdonalds comment. Yes it is all about marketing. People, students , most individuals do not know about Histology. They barely know what the word means, they usually think that its some kind of study of history (go figure). > > I have meet so many students repeatedly (biology, medical technology, chemistry > majors) who love the idea of a career in histotechnology. Especially after finding out the many options there are besides being in a high volume lab cutting 200-300 blocks per day if not more. > > If people dont know...we dont grow. > Till then..> > Sincerely,> JDhisto> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Your PC, mobile phone, and online services work together like never before. http://clk.atdmt.com/MRT/go/108587394/direct/01/ ------------------------------ Message: 8 Date: Thu, 07 Aug 2008 09:18:32 -0400 From: "Anthony Boris" Subject: [Histonet] Job Opening To: Message-ID: <489ABDE8.7813.00DE.0@trinity-health.org> Content-Type: text/plain; charset=US-ASCII St Joseph Mercy Oakland in Pontiac, Michigan has a full time opening for a HT or HTL (ASCP). Registry eligible and new grads are encouraged to apply. It is a day shift position, Monday-Friday. Candidate must be proficient in routine histology skills. Special stain and Immunohistochemistry experience is desired. The wage scale is highly aggressive and excellent benefits are offered. St Joes even has a free concierge service that will run your errands while you are at work! Please apply online at http://sjmoweb.trinity-health.org/. or call 248-858-6231 for more info Thanks Tony Boris ------------------------------ Message: 9 Date: Thu, 07 Aug 2008 08:27:26 -0500 From: "Matthew Roark" Subject: [Histonet] Coverslipper To: Message-ID: <489AB1EE02000011000665B6@email_gateway.sfmc.net> Content-Type: text/plain; charset=US-ASCII We are currently in the market for a new coverslipper. What are you using and/or what would everyone recommend? We currently have an old Leica glass coverslipper but would be open to try something new. Thanks! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 ------------------------------ Message: 10 Date: Thu, 7 Aug 2008 06:44:18 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Coverslipper To: Histonet@lists.utsouthwestern.edu, Matthew Roark Message-ID: <274640.66247.qm@web65710.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Try the Sakura film, or if you?prefer glass, the Sakura glass coverslipper. Ren? J. --- On Thu, 8/7/08, Matthew Roark wrote: From: Matthew Roark Subject: [Histonet] Coverslipper To: Histonet@lists.utsouthwestern.edu Date: Thursday, August 7, 2008, 9:27 AM We are currently in the market for a new coverslipper. What are you using and/or what would everyone recommend? We currently have an old Leica glass coverslipper but would be open to try something new. Thanks! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Thu, 7 Aug 2008 06:33:43 -0700 From: "Masterson_John" Subject: [Histonet] GMA 1 micron section staining To: "Anthony Boris" , Message-ID: <0C58C4F16F0B67448318A38041CADE4B01594F26@IRMAIL133.irvine.allergan.com> Content-Type: text/plain; charset="us-ascii" Hello Histonetters, I need to stain some 1 micron sections of GMA embedded tissue for H&E. Does anyone have experience they can share? Thanks in advance. John ------------------------------ Message: 12 Date: Thu, 7 Aug 2008 09:48:27 -0400 From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" Subject: RE: [Histonet] Coverslipper To: "Matthew Roark" , Histonet@lists.utsouthwestern.edu Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70208BF30@LTA3VS011.ees.hhs.gov> Content-Type: text/plain; charset=us-ascii We have one of the newer Leica's and have just recently ordered one of Sakura's as well. We have not yet tried the Sakura brand. We have to have glass and have been very pleased with the Leica. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Roark Sent: Thursday, August 07, 2008 9:27 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslipper We are currently in the market for a new coverslipper. What are you using and/or what would everyone recommend? We currently have an old Leica glass coverslipper but would be open to try something new. Thanks! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Thu, 7 Aug 2008 09:01:59 -0500 From: "Mike Pence" Subject: RE: [Histonet] Coverslipper To: "Matthew Roark" , Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A38CB@IS-E2K3.grhs.net> Content-Type: text/plain; charset="us-ascii" I love our Sakura film. Have had no problems with it in over 10 years, just general maintenance. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Roark Sent: Thursday, August 07, 2008 8:27 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslipper We are currently in the market for a new coverslipper. What are you using and/or what would everyone recommend? We currently have an old Leica glass coverslipper but would be open to try something new. Thanks! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Thu, 7 Aug 2008 09:12:07 -0500 From: Jackie M O'Connor Subject: RE: [Histonet] Coverslipper To: "Mike Pence" Cc: Histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" We have been using the Sakura film coverslipper for over a year - slides can be put into slide racks within minutes after coverslipping. Great when you have to turn slides around or mail them out quickly. Jackie O' "Mike Pence" Sent by: histonet-bounces@lists.utsouthwestern.edu 08/07/2008 09:01 AM To "Matthew Roark" , cc Subject RE: [Histonet] Coverslipper I love our Sakura film. Have had no problems with it in over 10 years, just general maintenance. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Roark Sent: Thursday, August 07, 2008 8:27 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslipper We are currently in the market for a new coverslipper. What are you using and/or what would everyone recommend? We currently have an old Leica glass coverslipper but would be open to try something new. Thanks! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Thu, 7 Aug 2008 09:12:31 -0600 From: "Gayle Callis" Subject: Re: [Histonet] GMA 1 micron section staining To: "Masterson_John" , "Anthony Boris" , Message-ID: <001d01c8f8a0$035fb980$6501a8c0@Sunney> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Do not rinse but put dry sections directly to Gill 3 hematoxylin 10 minutes. Time can vary. Rinse 3 X with distilled water Go directly to bluing 1 min Scotts Tap water substitute Rinse with distilled water Air dry sections Eosin/phloxine for 2 to 5 minutes time will vary. Rinse very quickly through 2 changes of 95% ethanol only and air dry quickly. I liked to use compressed or forced air, a fan works nicely. Coverslip with permanent mounting media over dry section. Be aware that 1 um is really thin and will not look like a regular H&E on a thicker section. There just isn't much tissue in a 1 um thick section. I had pathologists complain, but when this was explained, they understood what was going on since they were used to looking at 5 um thick sections. Alcohols during dehyration will cause sections to release or bulge from slide. Xylene can create problems too so we always mounted a coverglass over a dry section. You can thin the mounting media a bit just in case it doesn't flow well. If you have a bit of funky plastic fold over of plastic, that can be scraped away with a teflon coated razor blade, to not have extra thickness in a bad place during coverslipping. We did not use Harris hematoxylin for GMA sections, and this method basically is what Polysciences put out years ago when GMA first came out, and it worked very well for us over the years. Good luck on staining Gayle M. Callis HTL/HT/MT(ASCP) ----- Original Message ----- From: "Masterson_John" To: "Anthony Boris" ; Sent: Thursday, August 07, 2008 7:33 AM Subject: [Histonet] GMA 1 micron section staining Hello Histonetters, I need to stain some 1 micron sections of GMA embedded tissue for H&E. Does anyone have experience they can share? Thanks in advance. John _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Thu, 7 Aug 2008 11:20:01 -0500 From: "Martinez, Angela" Subject: [Histonet] Job Opening In Ft. Worth Texas To: Cc: "Hale, Meredith" Message-ID: <9B8A3AC772C7F64680392A7CB8FBFB0F05549E1D@s-irv-ex301.PathologyPartners.intranet> Content-Type: text/plain; charset="us-ascii" Great opportunity for Histotechnician in brand new laboratory! Gastrointestinal Associates of North Texas in Ft Worth , is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and meet CLIA-88 regulations to perform gross dissection. Prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. The position offers competitive salary, medical insurance, retirement plan, and vacation /sick leave. Interested applicants should e-mail resumes to Meredith Hale at mhale@carisdx.com . Meredith Hale HT (ASCP) Technical Director Caris Diagnostics 8400 Esters Blvd. Ste. 190 Irving, Texas 75063 ------------------------------ Message: 17 Date: Thu, 7 Aug 2008 09:36:02 -0700 (PDT) From: Kim Merriam Subject: [Histonet] cryostat block holders To: Histonet Message-ID: <611208.37592.qm@web50305.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi everyone, I want to order some disposable cryostat block holders (the round things that you attach your block to).? I?don't know what they are officially called, so I am having trouble searching for them.??Does anyone know what they are called and if anyone sells?plastic ones? Thanks, Kim ?Kim Merriam, MA, HT(ASCP) Cambridge, MA ------------------------------ Message: 18 Date: Thu, 07 Aug 2008 12:43:14 -0400 From: Mary Ascenzi Subject: [Histonet] camera for microscope To: histonet@lists.utsouthwestern.edu Message-ID: <6.2.1.2.2.20080807123426.01e0a900@postoffice6.mail.cornell.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Hello All I have an old Olympus microscope and am looking for a digital camera for photomics of slides. I know that there are cameras designed specifically for microscopes, but am wondering if anyone has used a digital SLR with an adapter. What is the photo quality, ie sharpness, color fidelity ? What software do you use ? Is Photoshop adequate? thanks, Mary ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 57, Issue 13 **************************************** ---------------------------------------------------------------------- NOTICE: This message is confidential, intended for the named recipient(s) and may contain information that is (i) proprietary to the sender, and/or,(ii) privileged, confidential and/or otherwise exempt from disclosure under applicable Florida and federal law, including, but not limited to, privacy standards imposed pursuant to the federal Health insurance Portability and Accountability Act of 1996 ("HIPAA"). Receipt by anyone other than the named recipient(s) is not a waiver of any applicable privilege. Thank you in advance for your compliance with this notice. From trathborne <@t> somerset-healthcare.com Thu Aug 7 16:54:38 2008 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Aug 7 16:54:46 2008 Subject: [Histonet] RE: Coverslipper In-Reply-To: <4E59E1E7B5A80F448F47424C8A2FDEDB02295CA4@BHEXCHVS02.BH.LOCAL> Message-ID: We have the same. Contact your Leica service dept. We have a great service rep who has a list of what mounting mediums work with which clearants, and what setting the dispenser should be set at. Another thing to watch, is when you replace the bottle with the mounting medium, that may have bubbles in it. To avoid that, we refill ours as soon as we change to another bottle. This way, the newly filled bottle will have a few days to let the bubbles in it rise to the surface. I hope this helps. Toni Rathborne Pathology Supervisor Somerset Medical Center 110 Rehill Ave. Somerville,NJ 08876 908-595-2367 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Pemberton, Susan Sent: Thursday, August 07, 2008 5:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Coverslipper We have a Leica stainer/coverslipper - it's two years old. We like it, but we are having trouble with bubbles and can't seem to adjust them out. Any suggestions? Susan Pemberton MS,MT(ASCP)SM Laboratory Administrative Director Baptist Health 800 Prudential Drive Jacksonville, FL 32207 904.202.2016 Fax:904.202.2795 susan.pemberton@bmcjax.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, August 07, 2008 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 57, Issue 13 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Horse bones (Maxim_71@mail.ru) 2. RE: protein block (Sally Price) 3. Re: surveillance cameras in the lab (Dawn Cowie) 4. NSH class (Amber McKenzie) 5. Meditech Help (Walter, Janelle) 6. Poster Judges Needed (Cheryl Crowder) 7. RE: HT schools (joelle weaver) 8. Job Opening (Anthony Boris) 9. Coverslipper (Matthew Roark) 10. Re: Coverslipper (Rene J Buesa) 11. GMA 1 micron section staining (Masterson_John) 12. RE: Coverslipper (Bartlett, Jeanine (CDC/CCID/NCZVED)) 13. RE: Coverslipper (Mike Pence) 14. RE: Coverslipper (Jackie M O'Connor) 15. Re: GMA 1 micron section staining (Gayle Callis) 16. Job Opening In Ft. Worth Texas (Martinez, Angela) 17. cryostat block holders (Kim Merriam) 18. camera for microscope (Mary Ascenzi) ---------------------------------------------------------------------- Message: 1 Date: Wed, 6 Aug 2008 21:17:28 +0400 From: Maxim_71@mail.ru Subject: Re: [Histonet] Horse bones To: BERGERJL@aol.com Cc: histonet@lists.utsouthwestern.edu Message-ID: <1446850633.20080806211728@mail.ru> Content-Type: text/plain; charset=us-ascii R. Berger: I was not be able to get good quality sections from bone specimens, until begann to use next things: 1- determination endpoint of decalcification 2- isopropanol as dehydratant 3- mineral oil as antemedium before parafiin infiltration. In my personal experience only these things had great improvement for quality of my bone sections. All rest things have had minimal importance for me. I must use manual processing, because have not any processor. Sincerely, Maxim Peshkov, Russia, Taganrog. -----Original message--- > Date: Tue, 5 Aug 2008 20:07:50 EDT > From: BERGERJL@aol.com > Subject: [Histonet] Horse bones > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset="US-ASCII" > > We are trying to section pieces of horse femurs, > fetlock and carpal joints. > They have been decalcified with either HCl decal or > formic acid decal. > Before routine processing the bones appear to be > decalcified, but at sectioning > they are very hard and brittle (even after surface > decal) and chip out of the > paraffin block. > Any suggestions would be helpful. A pathologist > suggested a soap soaking > solution but could not remember the name of the soap. > > Help and thank you, > > R. Berger, HT ------------------------------ Message: 2 Date: Wed, 6 Aug 2008 13:24:45 -0400 From: "Sally Price" Subject: RE: [Histonet] protein block To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Gene: What you've decribed is not, in my expericence, that uncommon. It all depends on the blocking agent your using -- some protein blocks are made from casein or digested immunoglobulin (e.g Fab2 fargments), some are made with only animal serum, and others are combinations of these materials -- If the reagent you're using is made with animal serum, for example, the backgroud could be caused by the blocking agent sticking to endogenous immunoglobulins with the specimen. At least this is one possibility, and the only way to confirm/resolve this issue is to try a differnt type of protein blocker. Cheers, Sally ------------------------------ Message: 16 Date: Tue, 05 Aug 2008 05:49:59 -0400 From: njoydobro@aol.com Subject: [Histonet] protein block To: Histonet@lists.utsouthwestern.edu Good Morning everyone, ???? We are currently working up a new antibody and are trying it with and without protein block.? We are getting some unexpected results and I would like to see if anyone has seen similar outcomes.? We are getting less background without the protein block and the staining appears "crisper".? Anyone experienced this? Thanks, Gene ------------------------------ Message: 3 Date: Wed, 6 Aug 2008 11:29:23 -0700 (PDT) From: Dawn Cowie Subject: Re: [Histonet] surveillance cameras in the lab To: pruegg@ihctech.net, "histonet@lists.utsouthwestern.edu" , Amos Brooks Message-ID: <430954.54820.qm@web45013.mail.sp1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I didn't read the initial posts on this topic so I don't know why the lab has installed cameras. I will say that if it is because of problem employees, you get back to the fact of having poor management and supervision in place. ? Dawn Cowie, HT --- On Tue, 8/5/08, Amos Brooks wrote: From: Amos Brooks Subject: [Histonet] surveillance cameras in the lab To: pruegg@ihctech.net, "histonet@lists.utsouthwestern.edu" Date: Tuesday, August 5, 2008, 9:23 AM Patsy, I have to disagree with you there. I understand the need for security, but there is a certain amount of trust lost when one makes the decision to monitor employees without even informing them as to why. (Weather or not they are legally bound to do so.) You can accomplish the same thing by letting employees know that there is a problem (or a potential problem) and having them beware and observant. As a last resort when they find the problem still presists using stromger methods becomes warranted. Once you give up this personal liberty and allow someone to watch over your shoulder all the time you loose the ability to make the right choices as there are no choices to make. I would rather live in a society of people that want to do the right thing than one of people that are forced to. At that point we might as well allow those that would do us harm to make all the decisions for us and abandon any form of personal freedom we may have. Ben Franklin published in Poor Richard's Almanac the famous quote *"Those who would give up essential liberty to purchase a little temporary safety, deserve neither liberty nor safety."* It is important to temper our fears with a sense of who we are lest we devolve into a military state. Thanks, Amos Brooks Message: 19 Date: Mon, 4 Aug 2008 10:46:26 -0600 From: "Patsy Ruegg" Subject: RE: [Histonet] surveillance cameras in the lab To: "'Cheri Miller'" , , Message-ID: Content-Type: text/plain; charset="us-ascii" Come on. In these times of terror concerns I am not sure I would work in a place where I did not feel secure and the use of these devices help in that matter. We built a brand new University of Colorado Health Sciences Center and there are cameras all over the place as well as lock down. If you do not have an access card you cannot get into the labs. This was a pain at first but with all the crazy's we have to worry about out there it now makes me feel better. I just read in the paper this morning about a researcher whose house was bombed by Peta types for doing animal research, and we have had all sorts of disturbances over the years with precious research animals being released, protests, etc. Patsy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Wed, 6 Aug 2008 14:28:35 -0500 From: "Amber McKenzie" Subject: [Histonet] NSH class To: Message-ID: <03C921A1EAF7F541B16543F6EC6A4B3701D3B5C8@giamail2.Gia.com> Content-Type: text/plain; charset="US-ASCII" I am not able to attend the NSH convention this year, and I was looking at the Program guide...on Sunday from 12:30-4pm is a class "How to Start an accredited Histotech Program" by Peggy Wenk...anyone know how/if I could get the information from that class? Amber McKenzie, B.S., HT (ASCP) 1405 N. State St., Suite 400 Jackson, MS 39202 (ph) 601-863-0388 (fax) 601-326-3532 ------------------------------ Message: 5 Date: Wed, 6 Aug 2008 15:30:13 -0400 From: "Walter, Janelle" Subject: [Histonet] Meditech Help To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Is there anyone who I can talk to who has had experience setting up (or even using) the Meditech PTH module for Histology & Cytology? We are currently using CoPath, but have been 'sweet-talked' into changing to Meditech. Any help would be greatly appreciated. Thanks, Janelle Janelle D. Walter, BS MT(ASCP) Clinical Analyst, MIS Hanover Hospital 300 Highland Avenue Hanover, PA 17331 717-646-6899 fax:717-633-3521 walterj@hanoverhospital.org CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please respond immediately by returning this e-mail to the sender and destroying all copies of this communication including any attachments. ------------------------------ Message: 6 Date: Wed, 06 Aug 2008 16:45:16 -0500 From: "Cheryl Crowder" Subject: [Histonet] Poster Judges Needed To: "Histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" Due to "circumstances beyond our control", 2 of the judged for the NSH Poster Committee will be unable to attend the Symposium/Convention in Pittsburgh. So, as chair of the committee, I am looking for 2 volunteers to judge posters. Qualifications include NSH membership and attendance at the Pittsburgh meeting and knowledge of clinical, research and educational histology. You don't have to have worked in all areas to be familiar with work done by others. A working knowledge of publication methods is helpful. The "job" requires that you talk to the presenters Sunday between 10:30 and 12:30, review the posters and judge them in specific areas sometime between Sunday and Monday afternoon, compile your results (that doesn't take long) and meet with the other members Monday afternoon. For all this you do not get paid, stay essentially anonymous and blow you mind from reviewing outstanding work by our members. If you are interested, please contact me as soon as possible by e-mail or phone. As someone said, it's the hardest job you'll ever love. Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 ------------------------------ Message: 7 Date: Wed, 6 Aug 2008 22:44:32 +0000 From: joelle weaver Subject: RE: [Histonet] HT schools To: , JD Message-ID: Content-Type: text/plain; charset="iso-8859-1" Those of us who particpate and work in Histology education, do as much as we can! I know that I put in about 20-30 hours a week plus my FT histology job trying to promote histology and being a histotech. For the most part, I get ignored. But just takes MORE people. LOTS more people to particpate. JMW > From: jdhisto@yahoo.com> Date: Tue, 5 Aug 2008 04:04:13 -0500> To: Histonet@lists.utsouthwestern.edu> CC: > Subject: [Histonet] HT schools> > Hello,> I totally agree with Jennifer Mcdonalds comment. Yes it is all about marketing. People, students , most individuals do not know about Histology. They barely know what the word means, they usually think that its some kind of study of history (go figure). > > I have meet so many students repeatedly (biology, medical technology, chemistry > majors) who love the idea of a career in histotechnology. Especially after finding out the many options there are besides being in a high volume lab cutting 200-300 blocks per day if not more. > > If people dont know...we dont grow. > Till then..> > Sincerely,> JDhisto> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Your PC, mobile phone, and online services work together like never before. http://clk.atdmt.com/MRT/go/108587394/direct/01/ ------------------------------ Message: 8 Date: Thu, 07 Aug 2008 09:18:32 -0400 From: "Anthony Boris" Subject: [Histonet] Job Opening To: Message-ID: <489ABDE8.7813.00DE.0@trinity-health.org> Content-Type: text/plain; charset=US-ASCII St Joseph Mercy Oakland in Pontiac, Michigan has a full time opening for a HT or HTL (ASCP). Registry eligible and new grads are encouraged to apply. It is a day shift position, Monday-Friday. Candidate must be proficient in routine histology skills. Special stain and Immunohistochemistry experience is desired. The wage scale is highly aggressive and excellent benefits are offered. St Joes even has a free concierge service that will run your errands while you are at work! Please apply online at http://sjmoweb.trinity-health.org/. or call 248-858-6231 for more info Thanks Tony Boris ------------------------------ Message: 9 Date: Thu, 07 Aug 2008 08:27:26 -0500 From: "Matthew Roark" Subject: [Histonet] Coverslipper To: Message-ID: <489AB1EE02000011000665B6@email_gateway.sfmc.net> Content-Type: text/plain; charset=US-ASCII We are currently in the market for a new coverslipper. What are you using and/or what would everyone recommend? We currently have an old Leica glass coverslipper but would be open to try something new. Thanks! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 ------------------------------ Message: 10 Date: Thu, 7 Aug 2008 06:44:18 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Coverslipper To: Histonet@lists.utsouthwestern.edu, Matthew Roark Message-ID: <274640.66247.qm@web65710.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Try the Sakura film, or if you?prefer glass, the Sakura glass coverslipper. Ren? J. --- On Thu, 8/7/08, Matthew Roark wrote: From: Matthew Roark Subject: [Histonet] Coverslipper To: Histonet@lists.utsouthwestern.edu Date: Thursday, August 7, 2008, 9:27 AM We are currently in the market for a new coverslipper. What are you using and/or what would everyone recommend? We currently have an old Leica glass coverslipper but would be open to try something new. Thanks! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Thu, 7 Aug 2008 06:33:43 -0700 From: "Masterson_John" Subject: [Histonet] GMA 1 micron section staining To: "Anthony Boris" , Message-ID: <0C58C4F16F0B67448318A38041CADE4B01594F26@IRMAIL133.irvine.allergan.com> Content-Type: text/plain; charset="us-ascii" Hello Histonetters, I need to stain some 1 micron sections of GMA embedded tissue for H&E. Does anyone have experience they can share? Thanks in advance. John ------------------------------ Message: 12 Date: Thu, 7 Aug 2008 09:48:27 -0400 From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" Subject: RE: [Histonet] Coverslipper To: "Matthew Roark" , Histonet@lists.utsouthwestern.edu Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70208BF30@LTA3VS011.ees.hhs.gov> Content-Type: text/plain; charset=us-ascii We have one of the newer Leica's and have just recently ordered one of Sakura's as well. We have not yet tried the Sakura brand. We have to have glass and have been very pleased with the Leica. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Roark Sent: Thursday, August 07, 2008 9:27 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslipper We are currently in the market for a new coverslipper. What are you using and/or what would everyone recommend? We currently have an old Leica glass coverslipper but would be open to try something new. Thanks! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Thu, 7 Aug 2008 09:01:59 -0500 From: "Mike Pence" Subject: RE: [Histonet] Coverslipper To: "Matthew Roark" , Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A38CB@IS-E2K3.grhs.net> Content-Type: text/plain; charset="us-ascii" I love our Sakura film. Have had no problems with it in over 10 years, just general maintenance. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Roark Sent: Thursday, August 07, 2008 8:27 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslipper We are currently in the market for a new coverslipper. What are you using and/or what would everyone recommend? We currently have an old Leica glass coverslipper but would be open to try something new. Thanks! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Thu, 7 Aug 2008 09:12:07 -0500 From: Jackie M O'Connor Subject: RE: [Histonet] Coverslipper To: "Mike Pence" Cc: Histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" We have been using the Sakura film coverslipper for over a year - slides can be put into slide racks within minutes after coverslipping. Great when you have to turn slides around or mail them out quickly. Jackie O' "Mike Pence" Sent by: histonet-bounces@lists.utsouthwestern.edu 08/07/2008 09:01 AM To "Matthew Roark" , cc Subject RE: [Histonet] Coverslipper I love our Sakura film. Have had no problems with it in over 10 years, just general maintenance. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Roark Sent: Thursday, August 07, 2008 8:27 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslipper We are currently in the market for a new coverslipper. What are you using and/or what would everyone recommend? We currently have an old Leica glass coverslipper but would be open to try something new. Thanks! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Thu, 7 Aug 2008 09:12:31 -0600 From: "Gayle Callis" Subject: Re: [Histonet] GMA 1 micron section staining To: "Masterson_John" , "Anthony Boris" , Message-ID: <001d01c8f8a0$035fb980$6501a8c0@Sunney> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Do not rinse but put dry sections directly to Gill 3 hematoxylin 10 minutes. Time can vary. Rinse 3 X with distilled water Go directly to bluing 1 min Scotts Tap water substitute Rinse with distilled water Air dry sections Eosin/phloxine for 2 to 5 minutes time will vary. Rinse very quickly through 2 changes of 95% ethanol only and air dry quickly. I liked to use compressed or forced air, a fan works nicely. Coverslip with permanent mounting media over dry section. Be aware that 1 um is really thin and will not look like a regular H&E on a thicker section. There just isn't much tissue in a 1 um thick section. I had pathologists complain, but when this was explained, they understood what was going on since they were used to looking at 5 um thick sections. Alcohols during dehyration will cause sections to release or bulge from slide. Xylene can create problems too so we always mounted a coverglass over a dry section. You can thin the mounting media a bit just in case it doesn't flow well. If you have a bit of funky plastic fold over of plastic, that can be scraped away with a teflon coated razor blade, to not have extra thickness in a bad place during coverslipping. We did not use Harris hematoxylin for GMA sections, and this method basically is what Polysciences put out years ago when GMA first came out, and it worked very well for us over the years. Good luck on staining Gayle M. Callis HTL/HT/MT(ASCP) ----- Original Message ----- From: "Masterson_John" To: "Anthony Boris" ; Sent: Thursday, August 07, 2008 7:33 AM Subject: [Histonet] GMA 1 micron section staining Hello Histonetters, I need to stain some 1 micron sections of GMA embedded tissue for H&E. Does anyone have experience they can share? Thanks in advance. John _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Thu, 7 Aug 2008 11:20:01 -0500 From: "Martinez, Angela" Subject: [Histonet] Job Opening In Ft. Worth Texas To: Cc: "Hale, Meredith" Message-ID: <9B8A3AC772C7F64680392A7CB8FBFB0F05549E1D@s-irv-ex301.PathologyPartners.intranet> Content-Type: text/plain; charset="us-ascii" Great opportunity for Histotechnician in brand new laboratory! Gastrointestinal Associates of North Texas in Ft Worth , is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and meet CLIA-88 regulations to perform gross dissection. Prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. The position offers competitive salary, medical insurance, retirement plan, and vacation /sick leave. Interested applicants should e-mail resumes to Meredith Hale at mhale@carisdx.com . Meredith Hale HT (ASCP) Technical Director Caris Diagnostics 8400 Esters Blvd. Ste. 190 Irving, Texas 75063 ------------------------------ Message: 17 Date: Thu, 7 Aug 2008 09:36:02 -0700 (PDT) From: Kim Merriam Subject: [Histonet] cryostat block holders To: Histonet Message-ID: <611208.37592.qm@web50305.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi everyone, I want to order some disposable cryostat block holders (the round things that you attach your block to).? I?don't know what they are officially called, so I am having trouble searching for them.??Does anyone know what they are called and if anyone sells?plastic ones? Thanks, Kim ?Kim Merriam, MA, HT(ASCP) Cambridge, MA ------------------------------ Message: 18 Date: Thu, 07 Aug 2008 12:43:14 -0400 From: Mary Ascenzi Subject: [Histonet] camera for microscope To: histonet@lists.utsouthwestern.edu Message-ID: <6.2.1.2.2.20080807123426.01e0a900@postoffice6.mail.cornell.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Hello All I have an old Olympus microscope and am looking for a digital camera for photomics of slides. I know that there are cameras designed specifically for microscopes, but am wondering if anyone has used a digital SLR with an adapter. What is the photo quality, ie sharpness, color fidelity ? What software do you use ? Is Photoshop adequate? thanks, Mary ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 57, Issue 13 **************************************** ---------------------------------------------------------------------- NOTICE: This message is confidential, intended for the named recipient(s) and may contain information that is (i) proprietary to the sender, and/or,(ii) privileged, confidential and/or otherwise exempt from disclosure under applicable Florida and federal law, including, but not limited to, privacy standards imposed pursuant to the federal Health insurance Portability and Accountability Act of 1996 ("HIPAA"). Receipt by anyone other than the named recipient(s) is not a waiver of any applicable privilege. Thank you in advance for your compliance with this notice. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Somerset Medical Center is proud to receive the Somerset County Business Partnership's 2006 Quality of Life Award. From jseaton <@t> wlgore.com Thu Aug 7 18:02:56 2008 From: jseaton <@t> wlgore.com (Janella Seaton) Date: Thu Aug 7 18:03:08 2008 Subject: [Histonet] Janella Seaton/WLGORE is out of the office. Message-ID: I will be out of the office starting 08/07/2008 and will not return until 08/12/2008. I will respond to your message when I return. From joelleweaver <@t> hotmail.com Thu Aug 7 18:26:42 2008 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Thu Aug 7 18:26:54 2008 Subject: [Histonet] cryostat block holders In-Reply-To: <586052.27945.qm@web65714.mail.ac4.yahoo.com> References: <611208.37592.qm@web50305.mail.re2.yahoo.com> <586052.27945.qm@web65714.mail.ac4.yahoo.com> Message-ID: Most people call them "chucks"- but I have seen them called "object holders" in vendor catalogs... > Date: Thu, 7 Aug 2008 13:26:31 -0700> From: rjbuesa@yahoo.com> To: histonet@lists.utsouthwestern.edu; kmerriam2003@yahoo.com> Subject: Re: [Histonet] cryostat block holders> CC: > > Chucks.> Ren? J.> > --- On Thu, 8/7/08, Kim Merriam wrote:> > From: Kim Merriam > Subject: [Histonet] cryostat block holders> To: "Histonet" > Date: Thursday, August 7, 2008, 12:36 PM> > Hi everyone,> I want to order some disposable cryostat block holders (the round things that> you attach your block to). I don't know what they are officially called,> so I am having trouble searching for them. Does anyone know what they are> called and if anyone sells plastic ones?> Thanks,> Kim> Kim Merriam, MA, HT(ASCP)> Cambridge, MA> > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Reveal your inner athlete and share it with friends on Windows Live. http://revealyourinnerathlete.windowslive.com?locale=en-us&ocid=TXT_TAGLM_WLYIA_whichathlete_us From ccrowder <@t> vetmed.lsu.edu Thu Aug 7 19:23:30 2008 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Thu Aug 7 19:27:44 2008 Subject: [Histonet] Thanks to the Histonet Message-ID: I want to thank all of you for answering my help for judges of the Posters. I have gotten many qualified techs willing to give of their time and energy for this committee. The Histonet has come through again. You're the greatest. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 From gayle.callis <@t> bresnan.net Thu Aug 7 22:03:00 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Thu Aug 7 22:03:08 2008 Subject: [Histonet] cryostat block holders References: <611208.37592.qm@web50305.mail.re2.yahoo.com><586052.27945.qm@web65714.mail.ac4.yahoo.com> Message-ID: <000a01c8f903$44ea6810$6501a8c0@Sunney> Dear Kim et al, I spent a good part of today looking for disposable "chucks", specimen object disks aka discs, etc, etc! All I found were these items under many different names , always metal, in different sizes and designs for specific for some cryostats. So much for industry standards, but I do still refer to them as chucks, long recycled from paraffin to cryostat use. I would be very interested if there is a company that makes disposable cryostat disks too. So far no show for the disposable items. We have found all metal disks without the O ring to be best for disinfection as the O ring is eventually eaten by chemicals. The ones I really like are the rectangular design with waffle weave, with a stem that fits into my beloved cryostats, and perfect for larger frozen blocks. Good luck Gayle M Callis HTL/HT/MT(ASCP) ----- Original Message ----- From: "joelle weaver" To: Sent: Thursday, August 07, 2008 5:26 PM Subject: RE: [Histonet] cryostat block holders Most people call them "chucks"- but I have seen them called "object holders" in vendor catalogs... > Date: Thu, 7 Aug 2008 13:26:31 -0700> From: rjbuesa@yahoo.com> To: > histonet@lists.utsouthwestern.edu; kmerriam2003@yahoo.com> Subject: Re: > [Histonet] cryostat block holders> CC: > > Chucks.> Ren? J.> > --- On Thu, > 8/7/08, Kim Merriam wrote:> > From: Kim Merriam > > Subject: [Histonet] cryostat block holders> To: > "Histonet" > Date: Thursday, August 7, > 2008, 12:36 PM> > Hi everyone,> I want to order some disposable cryostat > block holders (the round things that> you attach your block to). I don't > know what they are officially called,> so I am having trouble searching > for them. Does anyone know what they are> called and if anyone sells > plastic ones?> Thanks,> Kim> Kim Merriam, MA, HT(ASCP)> Cambridge, MA> > > > > _______________________________________________> Histonet mailing > list> Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > > _______________________________________________> Histonet mailing list> > Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Reveal your inner athlete and share it with friends on Windows Live. http://revealyourinnerathlete.windowslive.com?locale=en-us&ocid=TXT_TAGLM_WLYIA_whichathlete_us_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Fri Aug 8 05:29:19 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri Aug 8 05:34:05 2008 Subject: [Histonet] RE: Coverslipper In-Reply-To: <4E59E1E7B5A80F448F47424C8A2FDEDB02295CA4@BHEXCHVS02.BH.LOCAL> References: <200808071702.m77H2IdA024723@bhagent1.bmcjax.com> <4E59E1E7B5A80F448F47424C8A2FDEDB02295CA4@BHEXCHVS02.BH.LOCAL> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70208BF3D@LTA3VS011.ees.hhs.gov> We had them occasionally and were frequently adjusting volume, etc. We have the xylene bath and just realized we should not be using toluene-based mountant with it like we were. We switched to Cytoseal XL, which required a smaller dispensing nozzle, and that has taken care of it. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pemberton, Susan Sent: Thursday, August 07, 2008 5:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Coverslipper We have a Leica stainer/coverslipper - it's two years old. We like it, but we are having trouble with bubbles and can't seem to adjust them out. Any suggestions? Susan Pemberton MS,MT(ASCP)SM Laboratory Administrative Director Baptist Health 800 Prudential Drive Jacksonville, FL 32207 904.202.2016 Fax:904.202.2795 susan.pemberton@bmcjax.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, August 07, 2008 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 57, Issue 13 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Horse bones (Maxim_71@mail.ru) 2. RE: protein block (Sally Price) 3. Re: surveillance cameras in the lab (Dawn Cowie) 4. NSH class (Amber McKenzie) 5. Meditech Help (Walter, Janelle) 6. Poster Judges Needed (Cheryl Crowder) 7. RE: HT schools (joelle weaver) 8. Job Opening (Anthony Boris) 9. Coverslipper (Matthew Roark) 10. Re: Coverslipper (Rene J Buesa) 11. GMA 1 micron section staining (Masterson_John) 12. RE: Coverslipper (Bartlett, Jeanine (CDC/CCID/NCZVED)) 13. RE: Coverslipper (Mike Pence) 14. RE: Coverslipper (Jackie M O'Connor) 15. Re: GMA 1 micron section staining (Gayle Callis) 16. Job Opening In Ft. Worth Texas (Martinez, Angela) 17. cryostat block holders (Kim Merriam) 18. camera for microscope (Mary Ascenzi) ---------------------------------------------------------------------- Message: 1 Date: Wed, 6 Aug 2008 21:17:28 +0400 From: Maxim_71@mail.ru Subject: Re: [Histonet] Horse bones To: BERGERJL@aol.com Cc: histonet@lists.utsouthwestern.edu Message-ID: <1446850633.20080806211728@mail.ru> Content-Type: text/plain; charset=us-ascii R. Berger: I was not be able to get good quality sections from bone specimens, until begann to use next things: 1- determination endpoint of decalcification 2- isopropanol as dehydratant 3- mineral oil as antemedium before parafiin infiltration. In my personal experience only these things had great improvement for quality of my bone sections. All rest things have had minimal importance for me. I must use manual processing, because have not any processor. Sincerely, Maxim Peshkov, Russia, Taganrog. -----Original message--- > Date: Tue, 5 Aug 2008 20:07:50 EDT > From: BERGERJL@aol.com > Subject: [Histonet] Horse bones > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset="US-ASCII" > > We are trying to section pieces of horse femurs, fetlock and carpal > joints. > They have been decalcified with either HCl decal or formic acid > decal. > Before routine processing the bones appear to be decalcified, but at > sectioning they are very hard and brittle (even after surface > decal) and chip out of the > paraffin block. > Any suggestions would be helpful. A pathologist suggested a soap > soaking solution but could not remember the name of the soap. > > Help and thank you, > > R. Berger, HT ------------------------------ Message: 2 Date: Wed, 6 Aug 2008 13:24:45 -0400 From: "Sally Price" Subject: RE: [Histonet] protein block To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Gene: What you've decribed is not, in my expericence, that uncommon. It all depends on the blocking agent your using -- some protein blocks are made from casein or digested immunoglobulin (e.g Fab2 fargments), some are made with only animal serum, and others are combinations of these materials -- If the reagent you're using is made with animal serum, for example, the backgroud could be caused by the blocking agent sticking to endogenous immunoglobulins with the specimen. At least this is one possibility, and the only way to confirm/resolve this issue is to try a differnt type of protein blocker. Cheers, Sally ------------------------------ Message: 16 Date: Tue, 05 Aug 2008 05:49:59 -0400 From: njoydobro@aol.com Subject: [Histonet] protein block To: Histonet@lists.utsouthwestern.edu Good Morning everyone, ???? We are currently working up a new antibody and are trying it with and without protein block.? We are getting some unexpected results and I would like to see if anyone has seen similar outcomes.? We are getting less background without the protein block and the staining appears "crisper".? Anyone experienced this? Thanks, Gene ------------------------------ Message: 3 Date: Wed, 6 Aug 2008 11:29:23 -0700 (PDT) From: Dawn Cowie Subject: Re: [Histonet] surveillance cameras in the lab To: pruegg@ihctech.net, "histonet@lists.utsouthwestern.edu" , Amos Brooks Message-ID: <430954.54820.qm@web45013.mail.sp1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I didn't read the initial posts on this topic so I don't know why the lab has installed cameras. I will say that if it is because of problem employees, you get back to the fact of having poor management and supervision in place. ? Dawn Cowie, HT --- On Tue, 8/5/08, Amos Brooks wrote: From: Amos Brooks Subject: [Histonet] surveillance cameras in the lab To: pruegg@ihctech.net, "histonet@lists.utsouthwestern.edu" Date: Tuesday, August 5, 2008, 9:23 AM Patsy, I have to disagree with you there. I understand the need for security, but there is a certain amount of trust lost when one makes the decision to monitor employees without even informing them as to why. (Weather or not they are legally bound to do so.) You can accomplish the same thing by letting employees know that there is a problem (or a potential problem) and having them beware and observant. As a last resort when they find the problem still presists using stromger methods becomes warranted. Once you give up this personal liberty and allow someone to watch over your shoulder all the time you loose the ability to make the right choices as there are no choices to make. I would rather live in a society of people that want to do the right thing than one of people that are forced to. At that point we might as well allow those that would do us harm to make all the decisions for us and abandon any form of personal freedom we may have. Ben Franklin published in Poor Richard's Almanac the famous quote *"Those who would give up essential liberty to purchase a little temporary safety, deserve neither liberty nor safety."* It is important to temper our fears with a sense of who we are lest we devolve into a military state. Thanks, Amos Brooks Message: 19 Date: Mon, 4 Aug 2008 10:46:26 -0600 From: "Patsy Ruegg" Subject: RE: [Histonet] surveillance cameras in the lab To: "'Cheri Miller'" , , Message-ID: Content-Type: text/plain; charset="us-ascii" Come on. In these times of terror concerns I am not sure I would work in a place where I did not feel secure and the use of these devices help in that matter. We built a brand new University of Colorado Health Sciences Center and there are cameras all over the place as well as lock down. If you do not have an access card you cannot get into the labs. This was a pain at first but with all the crazy's we have to worry about out there it now makes me feel better. I just read in the paper this morning about a researcher whose house was bombed by Peta types for doing animal research, and we have had all sorts of disturbances over the years with precious research animals being released, protests, etc. Patsy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Wed, 6 Aug 2008 14:28:35 -0500 From: "Amber McKenzie" Subject: [Histonet] NSH class To: Message-ID: <03C921A1EAF7F541B16543F6EC6A4B3701D3B5C8@giamail2.Gia.com> Content-Type: text/plain; charset="US-ASCII" I am not able to attend the NSH convention this year, and I was looking at the Program guide...on Sunday from 12:30-4pm is a class "How to Start an accredited Histotech Program" by Peggy Wenk...anyone know how/if I could get the information from that class? Amber McKenzie, B.S., HT (ASCP) 1405 N. State St., Suite 400 Jackson, MS 39202 (ph) 601-863-0388 (fax) 601-326-3532 ------------------------------ Message: 5 Date: Wed, 6 Aug 2008 15:30:13 -0400 From: "Walter, Janelle" Subject: [Histonet] Meditech Help To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Is there anyone who I can talk to who has had experience setting up (or even using) the Meditech PTH module for Histology & Cytology? We are currently using CoPath, but have been 'sweet-talked' into changing to Meditech. Any help would be greatly appreciated. Thanks, Janelle Janelle D. Walter, BS MT(ASCP) Clinical Analyst, MIS Hanover Hospital 300 Highland Avenue Hanover, PA 17331 717-646-6899 fax:717-633-3521 walterj@hanoverhospital.org CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please respond immediately by returning this e-mail to the sender and destroying all copies of this communication including any attachments. ------------------------------ Message: 6 Date: Wed, 06 Aug 2008 16:45:16 -0500 From: "Cheryl Crowder" Subject: [Histonet] Poster Judges Needed To: "Histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" Due to "circumstances beyond our control", 2 of the judged for the NSH Poster Committee will be unable to attend the Symposium/Convention in Pittsburgh. So, as chair of the committee, I am looking for 2 volunteers to judge posters. Qualifications include NSH membership and attendance at the Pittsburgh meeting and knowledge of clinical, research and educational histology. You don't have to have worked in all areas to be familiar with work done by others. A working knowledge of publication methods is helpful. The "job" requires that you talk to the presenters Sunday between 10:30 and 12:30, review the posters and judge them in specific areas sometime between Sunday and Monday afternoon, compile your results (that doesn't take long) and meet with the other members Monday afternoon. For all this you do not get paid, stay essentially anonymous and blow you mind from reviewing outstanding work by our members. If you are interested, please contact me as soon as possible by e-mail or phone. As someone said, it's the hardest job you'll ever love. Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 ------------------------------ Message: 7 Date: Wed, 6 Aug 2008 22:44:32 +0000 From: joelle weaver Subject: RE: [Histonet] HT schools To: , JD Message-ID: Content-Type: text/plain; charset="iso-8859-1" Those of us who particpate and work in Histology education, do as much as we can! I know that I put in about 20-30 hours a week plus my FT histology job trying to promote histology and being a histotech. For the most part, I get ignored. But just takes MORE people. LOTS more people to particpate. JMW > From: jdhisto@yahoo.com> Date: Tue, 5 Aug 2008 04:04:13 -0500> To: Histonet@lists.utsouthwestern.edu> CC: > Subject: [Histonet] HT schools> > Hello,> I totally agree with Jennifer Mcdonalds comment. Yes it is all about marketing. People, students , most individuals do not know about Histology. They barely know what the word means, they usually think that its some kind of study of history (go figure). > > I have meet so many students repeatedly (biology, medical technology, chemistry > majors) who love the idea of a career in histotechnology. Especially after finding out the many options there are besides being in a high volume lab cutting 200-300 blocks per day if not more. > > If people dont know...we dont grow. > Till then..> > Sincerely,> JDhisto> _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Your PC, mobile phone, and online services work together like never before. http://clk.atdmt.com/MRT/go/108587394/direct/01/ ------------------------------ Message: 8 Date: Thu, 07 Aug 2008 09:18:32 -0400 From: "Anthony Boris" Subject: [Histonet] Job Opening To: Message-ID: <489ABDE8.7813.00DE.0@trinity-health.org> Content-Type: text/plain; charset=US-ASCII St Joseph Mercy Oakland in Pontiac, Michigan has a full time opening for a HT or HTL (ASCP). Registry eligible and new grads are encouraged to apply. It is a day shift position, Monday-Friday. Candidate must be proficient in routine histology skills. Special stain and Immunohistochemistry experience is desired. The wage scale is highly aggressive and excellent benefits are offered. St Joes even has a free concierge service that will run your errands while you are at work! Please apply online at http://sjmoweb.trinity-health.org/. or call 248-858-6231 for more info Thanks Tony Boris ------------------------------ Message: 9 Date: Thu, 07 Aug 2008 08:27:26 -0500 From: "Matthew Roark" Subject: [Histonet] Coverslipper To: Message-ID: <489AB1EE02000011000665B6@email_gateway.sfmc.net> Content-Type: text/plain; charset=US-ASCII We are currently in the market for a new coverslipper. What are you using and/or what would everyone recommend? We currently have an old Leica glass coverslipper but would be open to try something new. Thanks! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 ------------------------------ Message: 10 Date: Thu, 7 Aug 2008 06:44:18 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Coverslipper To: Histonet@lists.utsouthwestern.edu, Matthew Roark Message-ID: <274640.66247.qm@web65710.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Try the Sakura film, or if you?prefer glass, the Sakura glass coverslipper. Ren? J. --- On Thu, 8/7/08, Matthew Roark wrote: From: Matthew Roark Subject: [Histonet] Coverslipper To: Histonet@lists.utsouthwestern.edu Date: Thursday, August 7, 2008, 9:27 AM We are currently in the market for a new coverslipper. What are you using and/or what would everyone recommend? We currently have an old Leica glass coverslipper but would be open to try something new. Thanks! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Thu, 7 Aug 2008 06:33:43 -0700 From: "Masterson_John" Subject: [Histonet] GMA 1 micron section staining To: "Anthony Boris" , Message-ID: <0C58C4F16F0B67448318A38041CADE4B01594F26@IRMAIL133.irvine.allergan.com> Content-Type: text/plain; charset="us-ascii" Hello Histonetters, I need to stain some 1 micron sections of GMA embedded tissue for H&E. Does anyone have experience they can share? Thanks in advance. John ------------------------------ Message: 12 Date: Thu, 7 Aug 2008 09:48:27 -0400 From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" Subject: RE: [Histonet] Coverslipper To: "Matthew Roark" , Histonet@lists.utsouthwestern.edu Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70208BF30@LTA3VS011.ees.hhs.gov> Content-Type: text/plain; charset=us-ascii We have one of the newer Leica's and have just recently ordered one of Sakura's as well. We have not yet tried the Sakura brand. We have to have glass and have been very pleased with the Leica. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Roark Sent: Thursday, August 07, 2008 9:27 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslipper We are currently in the market for a new coverslipper. What are you using and/or what would everyone recommend? We currently have an old Leica glass coverslipper but would be open to try something new. Thanks! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Thu, 7 Aug 2008 09:01:59 -0500 From: "Mike Pence" Subject: RE: [Histonet] Coverslipper To: "Matthew Roark" , Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A38CB@IS-E2K3.grhs.net> Content-Type: text/plain; charset="us-ascii" I love our Sakura film. Have had no problems with it in over 10 years, just general maintenance. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Roark Sent: Thursday, August 07, 2008 8:27 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslipper We are currently in the market for a new coverslipper. What are you using and/or what would everyone recommend? We currently have an old Leica glass coverslipper but would be open to try something new. Thanks! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Thu, 7 Aug 2008 09:12:07 -0500 From: Jackie M O'Connor Subject: RE: [Histonet] Coverslipper To: "Mike Pence" Cc: Histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" We have been using the Sakura film coverslipper for over a year - slides can be put into slide racks within minutes after coverslipping. Great when you have to turn slides around or mail them out quickly. Jackie O' "Mike Pence" Sent by: histonet-bounces@lists.utsouthwestern.edu 08/07/2008 09:01 AM To "Matthew Roark" , cc Subject RE: [Histonet] Coverslipper I love our Sakura film. Have had no problems with it in over 10 years, just general maintenance. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Roark Sent: Thursday, August 07, 2008 8:27 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslipper We are currently in the market for a new coverslipper. What are you using and/or what would everyone recommend? We currently have an old Leica glass coverslipper but would be open to try something new. Thanks! Matthew Roark Histology Specialist -B.S,HT(ASCP)CM Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Thu, 7 Aug 2008 09:12:31 -0600 From: "Gayle Callis" Subject: Re: [Histonet] GMA 1 micron section staining To: "Masterson_John" , "Anthony Boris" , Message-ID: <001d01c8f8a0$035fb980$6501a8c0@Sunney> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Do not rinse but put dry sections directly to Gill 3 hematoxylin 10 minutes. Time can vary. Rinse 3 X with distilled water Go directly to bluing 1 min Scotts Tap water substitute Rinse with distilled water Air dry sections Eosin/phloxine for 2 to 5 minutes time will vary. Rinse very quickly through 2 changes of 95% ethanol only and air dry quickly. I liked to use compressed or forced air, a fan works nicely. Coverslip with permanent mounting media over dry section. Be aware that 1 um is really thin and will not look like a regular H&E on a thicker section. There just isn't much tissue in a 1 um thick section. I had pathologists complain, but when this was explained, they understood what was going on since they were used to looking at 5 um thick sections. Alcohols during dehyration will cause sections to release or bulge from slide. Xylene can create problems too so we always mounted a coverglass over a dry section. You can thin the mounting media a bit just in case it doesn't flow well. If you have a bit of funky plastic fold over of plastic, that can be scraped away with a teflon coated razor blade, to not have extra thickness in a bad place during coverslipping. We did not use Harris hematoxylin for GMA sections, and this method basically is what Polysciences put out years ago when GMA first came out, and it worked very well for us over the years. Good luck on staining Gayle M. Callis HTL/HT/MT(ASCP) ----- Original Message ----- From: "Masterson_John" To: "Anthony Boris" ; Sent: Thursday, August 07, 2008 7:33 AM Subject: [Histonet] GMA 1 micron section staining Hello Histonetters, I need to stain some 1 micron sections of GMA embedded tissue for H&E. Does anyone have experience they can share? Thanks in advance. John _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Thu, 7 Aug 2008 11:20:01 -0500 From: "Martinez, Angela" Subject: [Histonet] Job Opening In Ft. Worth Texas To: Cc: "Hale, Meredith" Message-ID: <9B8A3AC772C7F64680392A7CB8FBFB0F05549E1D@s-irv-ex301.PathologyPartners.intranet> Content-Type: text/plain; charset="us-ascii" Great opportunity for Histotechnician in brand new laboratory! Gastrointestinal Associates of North Texas in Ft Worth , is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and meet CLIA-88 regulations to perform gross dissection. Prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. The position offers competitive salary, medical insurance, retirement plan, and vacation /sick leave. Interested applicants should e-mail resumes to Meredith Hale at mhale@carisdx.com . Meredith Hale HT (ASCP) Technical Director Caris Diagnostics 8400 Esters Blvd. Ste. 190 Irving, Texas 75063 ------------------------------ Message: 17 Date: Thu, 7 Aug 2008 09:36:02 -0700 (PDT) From: Kim Merriam Subject: [Histonet] cryostat block holders To: Histonet Message-ID: <611208.37592.qm@web50305.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi everyone, I want to order some disposable cryostat block holders (the round things that you attach your block to).? I?don't know what they are officially called, so I am having trouble searching for them.??Does anyone know what they are called and if anyone sells?plastic ones? Thanks, Kim ?Kim Merriam, MA, HT(ASCP) Cambridge, MA ------------------------------ Message: 18 Date: Thu, 07 Aug 2008 12:43:14 -0400 From: Mary Ascenzi Subject: [Histonet] camera for microscope To: histonet@lists.utsouthwestern.edu Message-ID: <6.2.1.2.2.20080807123426.01e0a900@postoffice6.mail.cornell.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Hello All I have an old Olympus microscope and am looking for a digital camera for photomics of slides. I know that there are cameras designed specifically for microscopes, but am wondering if anyone has used a digital SLR with an adapter. What is the photo quality, ie sharpness, color fidelity ? What software do you use ? Is Photoshop adequate? thanks, Mary ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 57, Issue 13 **************************************** ---------------------------------------------------------------------- NOTICE: This message is confidential, intended for the named recipient(s) and may contain information that is (i) proprietary to the sender, and/or,(ii) privileged, confidential and/or otherwise exempt from disclosure under applicable Florida and federal law, including, but not limited to, privacy standards imposed pursuant to the federal Health insurance Portability and Accountability Act of 1996 ("HIPAA"). Receipt by anyone other than the named recipient(s) is not a waiver of any applicable privilege. Thank you in advance for your compliance with this notice. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Fri Aug 8 06:33:42 2008 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Fri Aug 8 06:33:48 2008 Subject: [Histonet] cryostat block holders In-Reply-To: <000a01c8f903$44ea6810$6501a8c0@Sunney> References: <611208.37592.qm@web50305.mail.re2.yahoo.com><586052.27945.qm@web65714.mail.ac4.yahoo.com> <000a01c8f903$44ea6810$6501a8c0@Sunney> Message-ID: Those are my favorite type as well. I have never seen any disposable types. Regards-Joelle> From: gayle.callis@bresnan.net> To: joelleweaver@hotmail.com; histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] cryostat block holders> Date: Thu, 7 Aug 2008 21:03:00 -0600> > Dear Kim et al,> > I spent a good part of today looking for disposable "chucks", specimen > object disks aka discs, etc, etc! All I found were these items under many > different names , always metal, in different sizes and designs for specific > for some cryostats. So much for industry standards, but I do still refer to > them as chucks, long recycled from paraffin to cryostat use.> > I would be very interested if there is a company that makes disposable > cryostat disks too. So far no show for the disposable items. We have found > all metal disks without the O ring to be best for disinfection as the O ring > is eventually eaten by chemicals. The ones I really like are the > rectangular design with waffle weave, with a stem that fits into my beloved > cryostats, and perfect for larger frozen blocks.> > Good luck> > Gayle M Callis> HTL/HT/MT(ASCP)> > ----- Original Message ----- > From: "joelle weaver" > To: > Sent: Thursday, August 07, 2008 5:26 PM> Subject: RE: [Histonet] cryostat block holders> > > > Most people call them "chucks"- but I have seen them called "object holders" > in vendor catalogs...> > Date: Thu, 7 Aug 2008 13:26:31 -0700> From: rjbuesa@yahoo.com> To: > > histonet@lists.utsouthwestern.edu; kmerriam2003@yahoo.com> Subject: Re: > > [Histonet] cryostat block holders> CC: > > Chucks.> Ren? J.> > --- On Thu, > > 8/7/08, Kim Merriam wrote:> > From: Kim Merriam > > > Subject: [Histonet] cryostat block holders> To: > > "Histonet" > Date: Thursday, August 7, > > 2008, 12:36 PM> > Hi everyone,> I want to order some disposable cryostat > > block holders (the round things that> you attach your block to). I don't > > know what they are officially called,> so I am having trouble searching > > for them. Does anyone know what they are> called and if anyone sells > > plastic ones?> Thanks,> Kim> Kim Merriam, MA, HT(ASCP)> Cambridge, MA> > > > > > _______________________________________________> Histonet mailing > > list> Histonet@lists.utsouthwestern.edu> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > > > _______________________________________________> Histonet mailing list> > > Histonet@lists.utsouthwestern.edu> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> _________________________________________________________________> Reveal your inner athlete and share it with friends on Windows Live.> http://revealyourinnerathlete.windowslive.com?locale=en-us&ocid=TXT_TAGLM_WLYIA_whichathlete_us_______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _________________________________________________________________ Your PC, mobile phone, and online services work together like never before. http://clk.atdmt.com/MRT/go/108587394/direct/01/ From petepath <@t> yahoo.com Fri Aug 8 06:53:34 2008 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Fri Aug 8 06:53:38 2008 Subject: [Histonet] cryostat block holders Message-ID: <678934.87678.qm@web45111.mail.sp1.yahoo.com> Hi Kim, ? We call those holders "chucks" or tissue stage. To my knowledge there are no plastic or disposable chucks used in cryostats.?Each cryostat has their?own design?that fit ?their particular chuck holder. I do?offer?very high quality stainless steel chucks??which ?fit most Leica and Microm cryostats.??If you?have been away from cryotomy for a while, my?web site contains a tutorial on frozen section technique which you may ?find helpful. I also offer an embedding system for frozen section which will allow you ?to precisely embed tissues for clinical and research methods. http://pathologyinnovations.com/index.html ? Let me know if? can help you in any way. ? Sincerely, ? Stephen? Stephen Peters M.D. Vice Chairman?of Pathology Hackensack University Medical Center 201 996 4836 ? Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com From jcbrinker4 <@t> charter.net Fri Aug 8 07:40:55 2008 From: jcbrinker4 <@t> charter.net (Jean Brinker) Date: Fri Aug 8 07:41:05 2008 Subject: [Histonet] New information system Message-ID: <000c01c8f954$00432a70$6401a8c0@JCBlaptop> Hi, Our Urology lab is searcing for a new information system to accommodate approximately 1400 surgical and 1800 cytology cases annually with the addition of FISH in the coming year. Some of the systems we have researched include Novopath, Pathologix, Intellipath (Netsoft), APEasy, and Webpath. I am looking for feedback from users of any of these systems. I would be particularly interested to hear from anyone using web based information systems. Also, we have found that the pricing information between systems to be complicated. Any advice here? I appreciated any and all feedback! Thank you, Jean From rshooki_99 <@t> yahoo.com Fri Aug 8 07:50:11 2008 From: rshooki_99 <@t> yahoo.com (richard shook) Date: Fri Aug 8 07:50:14 2008 Subject: [Histonet] uneven ihc staining Message-ID: <959783.10535.qm@web36107.mail.mud.yahoo.com> Im looking for help we are having uneven staining on some (not all) ihc slides we have looked into several different possoble reasons with no luck ,this happens a couple times every week ao so any suggestion would be great From lblazek <@t> digestivespecialists.com Fri Aug 8 07:56:44 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Aug 8 07:57:25 2008 Subject: [Histonet] New information system In-Reply-To: <000c01c8f954$00432a70$6401a8c0@JCBlaptop> References: <000c01c8f954$00432a70$6401a8c0@JCBlaptop> Message-ID: <5A2BD13465E061429D6455C8D6B40E390B77E373@IBMB7Exchange.digestivespecialists.com> I have been using the Pathlogix system for over two years and have found it and support to be excellent. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jean Brinker Sent: Friday, August 08, 2008 8:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New information system Hi, Our Urology lab is searcing for a new information system to accommodate approximately 1400 surgical and 1800 cytology cases annually with the addition of FISH in the coming year. Some of the systems we have researched include Novopath, Pathologix, Intellipath (Netsoft), APEasy, and Webpath. I am looking for feedback from users of any of these systems. I would be particularly interested to hear from anyone using web based information systems. Also, we have found that the pricing information between systems to be complicated. Any advice here? I appreciated any and all feedback! Thank you, Jean _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rfields <@t> gidocs.net Fri Aug 8 08:25:48 2008 From: rfields <@t> gidocs.net (Rosa Fields) Date: Fri Aug 8 08:25:56 2008 Subject: [Histonet] New information system In-Reply-To: <000c01c8f954$00432a70$6401a8c0@JCBlaptop> References: <000c01c8f954$00432a70$6401a8c0@JCBlaptop> Message-ID: <2F2611250DCD6549AA3D96CE8AF1F01801220A36@giexchange.gidocs.net> We use APEasy, and I really like it, the company has been great to work with, it is easily adaptable to how you wish to use it; you can contact me off list if you have any specific questions about the program. Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfields@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above.? If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited.? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jean Brinker Sent: Friday, August 08, 2008 7:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New information system Hi, Our Urology lab is searcing for a new information system to accommodate approximately 1400 surgical and 1800 cytology cases annually with the addition of FISH in the coming year. Some of the systems we have researched include Novopath, Pathologix, Intellipath (Netsoft), APEasy, and Webpath. I am looking for feedback from users of any of these systems. I would be particularly interested to hear from anyone using web based information systems. Also, we have found that the pricing information between systems to be complicated. Any advice here? I appreciated any and all feedback! Thank you, Jean _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mthomas <@t> littonlab.com Fri Aug 8 08:32:09 2008 From: mthomas <@t> littonlab.com (Marla Thomas) Date: Fri Aug 8 08:32:23 2008 Subject: [Histonet] New information system In-Reply-To: <000c01c8f954$00432a70$6401a8c0@JCBlaptop> References: <000c01c8f954$00432a70$6401a8c0@JCBlaptop> Message-ID: <000901c8f95b$28bec430$9d35a8c0@LittonPath.local> We use APEasy. We love it. They will customize the system to your needs. Their support is great, we have had the system for 10 years, you can't go wrong with them. Marla Thomas, HT(ASCP) HIPAA/Compliance/IT Manager Litton Pathology Associates, PC 700 NW Hunter Drive Blue Springs, MO 64015 816-229-6449, fax 816-874-4400 This e-mail, including attachments, may include confidential and/or proprietary information, and may be used only by the person or entity to which it is addressed. If the reader of this e-mail is not the intended recipient or his/her authorized agent, the reader is hereby notified that any dissemination, distribution or copying of this e-mail is prohibited. If you have received this e-mail in error, please notify the sender by replying to this message and delete this e-mail immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jean Brinker Sent: Friday, August 08, 2008 7:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New information system Hi, Our Urology lab is searcing for a new information system to accommodate approximately 1400 surgical and 1800 cytology cases annually with the addition of FISH in the coming year. Some of the systems we have researched include Novopath, Pathologix, Intellipath (Netsoft), APEasy, and Webpath. I am looking for feedback from users of any of these systems. I would be particularly interested to hear from anyone using web based information systems. Also, we have found that the pricing information between systems to be complicated. Any advice here? I appreciated any and all feedback! Thank you, Jean _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Fri Aug 8 08:32:10 2008 From: shive003 <@t> umn.edu (Jan Shivers) Date: Fri Aug 8 08:33:19 2008 Subject: [Histonet] uneven ihc staining References: <959783.10535.qm@web36107.mail.mud.yahoo.com> Message-ID: Assuming that you've checked to make sure that the tissues are completely covered with reagents during the entire staining process, here's the first things that pop into my head as possible answers: 1) tissue partially drying out on the trimming table 2) tissue not completely submerged in formalin after being trimmed in 3) tissue not completely fixed all the way through 4) tissue not completely deparaffinized 5) tissue undergoing faulty antigen retrieval step Jan Shivers ----- Original Message ----- From: "richard shook" To: Sent: Friday, August 08, 2008 7:50 AM Subject: [Histonet] uneven ihc staining > Im looking for help we are having uneven staining on some (not all) ihc > slides we have looked into several different possoble reasons with no luck > ,this happens a couple times every week ao so any suggestion would be > great > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Fri Aug 8 09:29:32 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 8 09:29:35 2008 Subject: [Histonet] uneven ihc staining In-Reply-To: <959783.10535.qm@web36107.mail.mud.yahoo.com> Message-ID: <337475.90470.qm@web65714.mail.ac4.yahoo.com> Most times this effect is caused by uneven dewaxing of the slides.You should have a very strict protocol for exchanging the dewaxing reagents. Ren? J. --- On Fri, 8/8/08, richard shook wrote: From: richard shook Subject: [Histonet] uneven ihc staining To: histonet@lists.utsouthwestern.edu Date: Friday, August 8, 2008, 8:50 AM Im looking for help we are having uneven staining on some (not all) ihc slides we have looked into several different possoble reasons with no luck ,this happens a couple times every week ao so any suggestion would be great _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jessica.Vacca <@t> HCAhealthcare.com Fri Aug 8 09:46:59 2008 From: Jessica.Vacca <@t> HCAhealthcare.com (Vacca Jessica) Date: Fri Aug 8 09:47:10 2008 Subject: [Histonet] converting to a new path system Message-ID: <41E16A15CE78374EA45B57E0F94339B8042F13A4@ORLEV01.hca.corpad.net> I have a hospital that is going from COPATH to Meditech. How have others in the past converted the patients hx to Meditech? Does the Path department keep COPATH up and running to access hx or do you just rely on Med records to keep file of the information? Going back from 1992. Med records only keeps 2 yrs in-house. Thanks in advance, Jessica From mike <@t> pathview.com Fri Aug 8 10:44:48 2008 From: mike <@t> pathview.com (Michael Mihalik) Date: Fri Aug 8 10:46:04 2008 Subject: [Histonet] converting to a new path system In-Reply-To: <41E16A15CE78374EA45B57E0F94339B8042F13A4@ORLEV01.hca.corpad.net> References: <41E16A15CE78374EA45B57E0F94339B8042F13A4@ORLEV01.hca.corpad.net> Message-ID: <006f01c8f96d$c3d66ba0$4b8342e0$@com> Isn't that part of the installation? Meditech 'should' convert your patient history from Copath. Your life is going to be very cumbersome if the history is not moved to your new system. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vacca Jessica Sent: Friday, August 08, 2008 10:47 AM To: histonet Subject: [Histonet] converting to a new path system I have a hospital that is going from COPATH to Meditech. How have others in the past converted the patients hx to Meditech? Does the Path department keep COPATH up and running to access hx or do you just rely on Med records to keep file of the information? Going back from 1992. Med records only keeps 2 yrs in-house. Thanks in advance, Jessica From Lynne.Bell <@t> hitchcock.org Fri Aug 8 11:00:54 2008 From: Lynne.Bell <@t> hitchcock.org (Bell, Lynne) Date: Fri Aug 8 11:01:01 2008 Subject: [Histonet] converting to a new path system In-Reply-To: <006f01c8f96d$c3d66ba0$4b8342e0$@com> Message-ID: We use Meditech in our hospital. Because our previous pathology system was designed by one of our pathologists, the Meditech conversion didn't happen smoothly. We still have access to the old pathology and cytology reports in another system. It is certainly a pain in the butt. I believe you can purchase the "conversion" package when you purchase Meditech. I would think that your current CoPath could be converted to Meditech. I would certainly ask the question of your IS department. Lynne Bell From tifei <@t> foxmail.com Fri Aug 8 11:21:38 2008 From: tifei <@t> foxmail.com (tf) Date: Fri Aug 8 11:21:56 2008 Subject: [Histonet] uneven ihc staining References: <959783.10535.qm@web36107.mail.mud.yahoo.com> Message-ID: <200808090021331791893@foxmail.com> bG9vayBhdCB1ciBtaWNyb3RvbWUgb3IgY3J5b3N0YXQNCnRyeSBIRSBzdGFpbmluZyBvciBvdGhl ciBzdGFpbmdzIHRvIHNlZSBpZiB0aGUgdGhpY2tuZXNzIG9mIHlvdXIgc2VjdGlvbiBpcyB1bmV2 ZW4NCg0KDQoyMDA4LTA4LTA5IA0KDQoNCg0KdGYgDQoNCg0KDQq3orz+yMujuiByaWNoYXJkIHNo b29rIA0Kt6LLzcqxvOSjuiAyMDA4LTA4LTA4ICAyMDo1MzoyMyANCsrVvP7Iy6O6IGhpc3RvbmV0 QGxpc3RzLnV0c291dGh3ZXN0ZXJuLmVkdSANCrOty82juiANCtb3zOKjuiBbSGlzdG9uZXRdIHVu ZXZlbiBpaGMgc3RhaW5pbmcgDQogDQpJbSBsb29raW5nIGZvciBoZWxwIHdlIGFyZSBoYXZpbmcg dW5ldmVuIHN0YWluaW5nIG9uIHNvbWUgKG5vdCBhbGwpIGloYyBzbGlkZXMgd2UgaGF2ZSBsb29r ZWQgaW50byBzZXZlcmFsIGRpZmZlcmVudCBwb3Nzb2JsZSByZWFzb25zIHdpdGggbm8gbHVjayAs dGhpcyBoYXBwZW5zIGEgY291cGxlIHRpbWVzIGV2ZXJ5IHdlZWsgYW8gc28gYW55IHN1Z2dlc3Rp b24gd291bGQgYmUgZ3JlYXQgDQogICAgICANCl9fX19fX19fX19fX19fX19fX19fX19fX19fX19f X19fX19fX19fX19fX19fX19fDQpIaXN0b25ldCBtYWlsaW5nIGxpc3QNCkhpc3RvbmV0QGxpc3Rz LnV0c291dGh3ZXN0ZXJuLmVkdQ0K From mike <@t> pathview.com Fri Aug 8 11:22:58 2008 From: mike <@t> pathview.com (Michael Mihalik) Date: Fri Aug 8 11:24:32 2008 Subject: [Histonet] converting to a new path system In-Reply-To: References: <006f01c8f96d$c3d66ba0$4b8342e0$@com> Message-ID: <009301c8f973$21f552a0$65dff7e0$@com> I"d even go so far to say that history conversion is one of the single most important aspects of procuring a new system. If you don't have history EASILY accessible on the new system, you either: 1. have to go to the old system for EVERY case and print the history for your pathologists to read, or 2. sometimes the history is converted, but it's 'separate' from the new system history. In that scenario you have to look 'elsewhere' in the new system for the patient history. Again, this is another real pain that you feel for every case. Not to toot our own horn so much, but that's why our history conversion always makes the converted case look just like you entered it on the new system. As a side benefit, when you go 'live', all the cases in progress are converted so that you can continue with them in the new system on day 1. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bell, Lynne Sent: Friday, August 08, 2008 12:01 PM To: Vacca Jessica; histonet Subject: RE: [Histonet] converting to a new path system We use Meditech in our hospital. Because our previous pathology system was designed by one of our pathologists, the Meditech conversion didn't happen smoothly. We still have access to the old pathology and cytology reports in another system. It is certainly a pain in the butt. I believe you can purchase the "conversion" package when you purchase Meditech. I would think that your current CoPath could be converted to Meditech. I would certainly ask the question of your IS department. Lynne Bell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Fri Aug 8 12:07:29 2008 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Aug 8 12:07:15 2008 Subject: [Histonet] Meditech Help In-Reply-To: Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F5A9@lmhsmail.lmhealth.org> So... not many Meditech users? We have used the PTH module since 1987, how can I help? Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Walter, Janelle Sent: Wednesday, August 06, 2008 3:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Meditech Help Is there anyone who I can talk to who has had experience setting up (or even using) the Meditech PTH module for Histology & Cytology? We are currently using CoPath, but have been 'sweet-talked' into changing to Meditech. Any help would be greatly appreciated. Thanks, Janelle Janelle D. Walter, BS MT(ASCP) Clinical Analyst, MIS Hanover Hospital 300 Highland Avenue Hanover, PA 17331 717-646-6899 fax:717-633-3521 walterj@hanoverhospital.org CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please respond immediately by returning this e-mail to the sender and destroying all copies of this communication including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMitchell <@t> uwhealth.org Fri Aug 8 12:18:56 2008 From: JMitchell <@t> uwhealth.org (Mitchell Jean A.) Date: Fri Aug 8 12:19:03 2008 Subject: [Histonet] Aqueous Mounting Media Message-ID: <713A63AAF4280941A2EF88D66838F9A002613165@uwhis-xchng4.uwhis.hosp.wisc.edu> Happy Friday!! I am looking for a replacement for Biomeda M01 Gel/Mount - aqueous mounting media (apparently this product is no longer available). My usage is for Oil Red O coverslipping and not fluorescence work. Would highly prefer a product that can be stored at room temp - and not required to be refrigerated. Any suggestions? Jean Mitchell, BS, HT (ASCP) University of Wisconsin Hospital & Clinics Neuromuscular Laboratory Manager 600 Highland Avenue Madison, WI 53792-5132 From katherine-walters <@t> uiowa.edu Fri Aug 8 12:24:03 2008 From: katherine-walters <@t> uiowa.edu (Walters, Katherine S) Date: Fri Aug 8 12:24:13 2008 Subject: [Histonet] Aqueous Mounting Media In-Reply-To: <713A63AAF4280941A2EF88D66838F9A002613165@uwhis-xchng4.uwhis.hosp.wisc.edu> Message-ID: I like Dako's Faramount. Kathy Walters Katherine Walters Histology Director Central Microscopy Research Facility University of Iowa 85 Eckstein Medical Research Building Iowa City, Iowa 52242-1101 phone: (319) 335-8142 fax: (319) 384-4469 katherine-walters@uiowa.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell Jean A. Sent: Friday, August 08, 2008 12:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Aqueous Mounting Media Happy Friday!! I am looking for a replacement for Biomeda M01 Gel/Mount - aqueous mounting media (apparently this product is no longer available). My usage is for Oil Red O coverslipping and not fluorescence work. Would highly prefer a product that can be stored at room temp - and not required to be refrigerated. Any suggestions? Jean Mitchell, BS, HT (ASCP) University of Wisconsin Hospital & Clinics Neuromuscular Laboratory Manager 600 Highland Avenue Madison, WI 53792-5132 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tanisha.mcknight <@t> covance.com Fri Aug 8 12:26:11 2008 From: tanisha.mcknight <@t> covance.com (McKnight, Tanisha) Date: Fri Aug 8 12:26:32 2008 Subject: [Histonet] Alternatives to Weigert's Iron Hematoxylin for Mucin and Trichrom e Staining Message-ID: <816E3C72F855F14985FC31D7C963AE6F08EC53FB@indexch03.ent.covance.com> Hello All: Can anyone suggest an alternative to Weigert's Iron Hematoxylin for nuclei staining with Mucin and Trichrome protocols? I have some standard hematoxylin (7211) that I will be using for my routine staining. However, with the shortage, Weigerts is on backorder until October. Tanisha McKnight, HT(ASCP) AP-Histology/Specimen Management Covance CLS, Indianapolis ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From talulahgosh <@t> gmail.com Fri Aug 8 12:28:11 2008 From: talulahgosh <@t> gmail.com (Emily Sours) Date: Fri Aug 8 12:28:16 2008 Subject: [Histonet] Aqueous Mounting Media In-Reply-To: <713A63AAF4280941A2EF88D66838F9A002613165@uwhis-xchng4.uwhis.hosp.wisc.edu> References: <713A63AAF4280941A2EF88D66838F9A002613165@uwhis-xchng4.uwhis.hosp.wisc.edu> Message-ID: Electron Microscopy Sciences bought the name and now makes Gel/Mount, cat #17985-10 Emily -- An overcivilized people grow complacent and careless and leave the door open for a tribe of fanatical savages, through a mixture of luck, treachery, and the foulest inhumanity, to usurp their place for a few years. -Richard Adams, "Shardik", 1974 From b-frederick <@t> northwestern.edu Fri Aug 8 13:48:24 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Aug 8 13:48:37 2008 Subject: [Histonet] uneven ihc staining In-Reply-To: <337475.90470.qm@web65714.mail.ac4.yahoo.com> Message-ID: <000001c8f987$59b2a440$d00f7ca5@lurie.northwestern.edu> Could also be a sensor issue on an autostainer or if the unit has been moved recently and not properly levelled. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, August 08, 2008 9:30 AM To: histonet@lists.utsouthwestern.edu; richard shook Subject: Re: [Histonet] uneven ihc staining Most times this effect is caused by uneven dewaxing of the slides.You should have a very strict protocol for exchanging the dewaxing reagents. Ren? J. --- On Fri, 8/8/08, richard shook wrote: From: richard shook Subject: [Histonet] uneven ihc staining To: histonet@lists.utsouthwestern.edu Date: Friday, August 8, 2008, 8:50 AM Im looking for help we are having uneven staining on some (not all) ihc slides we have looked into several different possoble reasons with no luck ,this happens a couple times every week ao so any suggestion would be great _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcbrinker4 <@t> charter.net Fri Aug 8 13:53:15 2008 From: jcbrinker4 <@t> charter.net (Jean Brinker) Date: Fri Aug 8 13:53:27 2008 Subject: [Histonet] Web based information systems Message-ID: <00bf01c8f988$03f55f60$6401a8c0@JCBlaptop> I would like to hear from someone using a web based information system. We are interested in the benefits and challenges unique to these systems. Thank you, Jean From Valerie.Hannen <@t> parrishmed.com Fri Aug 8 13:54:21 2008 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Fri Aug 8 13:55:01 2008 Subject: [Histonet] Meditech Help In-Reply-To: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F5A9@lmhsmail.lmhealth.org> Message-ID: <5680DA93771F0C48954CC8D38425E72401AB34E1@ISMAIL.parrishmed.local> We have used Meditech for many years as well. If I can be of any additional help, let me know. Valerie Hannen Parrish Medical Center Titusville,Florida -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Friday, August 08, 2008 1:07 PM To: Walter, Janelle; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Meditech Help So... not many Meditech users? We have used the PTH module since 1987, how can I help? Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Walter, Janelle Sent: Wednesday, August 06, 2008 3:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Meditech Help Is there anyone who I can talk to who has had experience setting up (or even using) the Meditech PTH module for Histology & Cytology? We are currently using CoPath, but have been 'sweet-talked' into changing to Meditech. Any help would be greatly appreciated. Thanks, Janelle Janelle D. Walter, BS MT(ASCP) Clinical Analyst, MIS Hanover Hospital 300 Highland Avenue Hanover, PA 17331 717-646-6899 fax:717-633-3521 walterj@hanoverhospital.org CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please respond immediately by returning this e-mail to the sender and destroying all copies of this communication including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************************************** "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you From rjbuesa <@t> yahoo.com Fri Aug 8 14:14:58 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 8 14:15:02 2008 Subject: [Histonet] Alternatives to Weigert's Iron Hematoxylin for Mucin and Trichrom e Staining In-Reply-To: <816E3C72F855F14985FC31D7C963AE6F08EC53FB@indexch03.ent.covance.com> Message-ID: <923452.75873.qm@web65710.mail.ac4.yahoo.com> Heidenhain's iron hematoxylin. Ren? J. --- On Fri, 8/8/08, McKnight, Tanisha wrote: From: McKnight, Tanisha Subject: [Histonet] Alternatives to Weigert's Iron Hematoxylin for Mucin and Trichrom e Staining To: histonet@lists.utsouthwestern.edu Date: Friday, August 8, 2008, 1:26 PM Hello All: Can anyone suggest an alternative to Weigert's Iron Hematoxylin for nuclei staining with Mucin and Trichrome protocols? I have some standard hematoxylin (7211) that I will be using for my routine staining. However, with the shortage, Weigerts is on backorder until October. Tanisha McKnight, HT(ASCP) AP-Histology/Specimen Management Covance CLS, Indianapolis ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sheila_adey <@t> hotmail.com Fri Aug 8 15:08:01 2008 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Fri Aug 8 15:08:05 2008 Subject: [Histonet] uneven ihc staining In-Reply-To: <000001c8f987$59b2a440$d00f7ca5@lurie.northwestern.edu> References: <337475.90470.qm@web65714.mail.ac4.yahoo.com> <000001c8f987$59b2a440$d00f7ca5@lurie.northwestern.edu> Message-ID: We had similar issues happening irregularly. We are now discarding the distilled H2O and TRIS buffer that the slides pre soak in before going on the instrument and after dewaxing. We haven't had any complaints since we started this. Sheila Adey HT MLTPort Huron HospitalMichigan> From: b-frederick@northwestern.edu> To: rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; rshooki_99@yahoo.com> Date: Fri, 8 Aug 2008 13:48:24 -0500> Subject: RE: [Histonet] uneven ihc staining> CC: > > Could also be a sensor issue on an autostainer or if the unit has been moved> recently and not properly levelled.> Bernice> > > Bernice Frederick HTL (ASCP)> Northwestern University> Pathology Core Facility> ECOGPCO-RL > 710 N Fairbanks Court> Olson 8-421> Chicago,IL 60611> 312-503-3723> > > -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa> Sent: Friday, August 08, 2008 9:30 AM> To: histonet@lists.utsouthwestern.edu; richard shook> Subject: Re: [Histonet] uneven ihc staining> > Most times this effect is caused by uneven dewaxing of the slides.You should> have a very strict protocol for exchanging the dewaxing reagents.> Ren? J.> > --- On Fri, 8/8/08, richard shook wrote:> > From: richard shook > Subject: [Histonet] uneven ihc staining> To: histonet@lists.utsouthwestern.edu> Date: Friday, August 8, 2008, 8:50 AM> > Im looking for help we are having uneven staining on some (not all) ihc> slides> we have looked into several different possoble reasons with no luck ,this> happens a couple times every week ao so any suggestion would be great > > > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Find hidden words, unscramble celebrity names, or try the ultimate crossword puzzle with Live Search Games. Play now! http://g.msn.ca/ca55/212 From lpwenk <@t> sbcglobal.net Fri Aug 8 20:11:00 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Aug 8 20:11:26 2008 Subject: [Histonet] Alternatives to Weigert's Iron Hematoxylin for Mucin andTrichrom e Staining In-Reply-To: <816E3C72F855F14985FC31D7C963AE6F08EC53FB@indexch03.ent.covance.com> Message-ID: <002201c8f9bc$c9a63520$0202a8c0@HPPav2> One of the problems with traditional Weigert Hematoxylin is that the ferric chloride (mordant) will also overoxidize the hematoxylin in 1-3 days, so it has to be made up every couple of days. This wastes our precious hematoxylin. Back in 1997, Histonet ran a Ferrous Hematoxylin, made with sodium iodate as the oxidizer, and ferrous sulfate and aluminum chloride as the mordants (and powdered hematoxylin, of course). Make it up, allow to oxidize 3 days at room temperature. Then store in the refrig. Reuse over and over until weak. One of my students tested this out, and used it every day, and it finally started to go weak about 3 months later. Make it on Friday, ready for use on Monday. This would be a good was to save on amount of hematoxylin. (Sorry Jim Elsam (who originally posted it) and Pete Jackson (whom Jim credits with giving him the procedure), but in our student lab, we call it Eva's hematoxylin, after our student who tested it out.) FERROUS HEMATOXYLIN (Procedure written by Beaumont Hospital, Royal Oak, MI) PURPOSE: This stain demonstrates nuclei. When used with Trichrome stains, it withstands decolorization of the nuclear staining longer than Weigert Hematoxylin. PRINCIPLE: The mordants are ferrous sulfate and aluminum chloride. The oxidizer is sodium iodate. Since this is an iron hematoxylin, the nuclei are black. However, since ferrous sulfate is a poor oxidizer, sodium iodate is used as an oxidizer. The sodium iodate slowly oxidizes the ferrous hematoxylin to ferrous hematein, so it must sit for about 3 days before use. However, it does not rapidly over-oxidize to ferrous oxy-hematein, so the solution will continue to stain nuclei black for about 2 months or longer. Hydrochloric acid is used to control the pH. FIXATION: Any well fixed tissue. TECHNIQUE: Cut paraffin sections at 5 um. CONTROL: Use the control for which the special stain is needed. QUALITY CONTROL: 1. Allow the solution to ripen for 3 days before use. 2. Store in the refrigerator, to slow down the rate of over-oxidation. CAUTION: Follow standard safety procedures when preparing stains. HEMATOXYLIN is incompatible with oxidizers and alkalies. Store separate from these. ALUMINUM CHLORIDE, HEXAHYDRATE is an irritant. Moisture sensitive, as hydrochloric acid could be created with the addition of water. FERROUS SULFATE, HEPTAHYDRATE is an irritant. Store dry chemical in refrigerator. Ingestion of high amounts can cause GI disturbances, liver damage, and even death. SODIUM IODATE is an oxidizer. Store away from other material. HYDROCHLORIC ACID is an acid. Add slowly, drop by drop, to solution. May cause severe skin and eye burns. REAGENTS: SOLUTION A - Ferrous Hematoxylin Hematoxylin (CI 75290) 0.5 g 95% Alcohol, reagent 50.0 mL Dissolve together. Store at room temperature. Stable for several months. SOLUTION B - Ferrous Hematoxylin Aluminum chloride, hexahydrate (AlCl3.6H2O) 5.0 g Ferrous sulfate, heptahydrate (FeSO4.7H2O) 5.0 g Distilled water 50.0 mL Dissolve together. Store at room temperature. Stable for 2 months. SOLUTION C - Ferrous Hematoxylin Sodium iodate (NaIO3) 0.9 g Distilled water 10.0 mL Dissolve together. Store at room temperature. Stable for several months. WORKING SOLUTION - FERROUS HEMATOXYLIN Solution A 25.0 mL Solution B 25.0 mL Mix together. Then add: Hydrochloric acid, concentrated (HCl) 0.5 mL Mix together. Then add: Solution C 0.5 mL Stir together. Allow to ripen for at room temperature a minimum of three days before use. Store in refrigerator (3o C.). May be reused until weak. Stable for 2-3 months, if used every day. May last longer if used less often. If use this stain for more than one coplin jar at a time, double the amount. PROCEDURE: 1. Use in place of Weigert Hematoxylin in any staining solution, for same amount of time. RESULTS: Nuclei black PROCEDURAL NOTES: 1. May be used cold. Usually do not need to extend time. 2. Allow to oxidize (ripen) for a minimum of three (3) days before using. Unoxidized (new) Ferrous Hematoxylin will not stain nuclei. 3. Store in refrigerator after it has ripened (Note #2), to extend life of stain. 4. Over-oxidized (old) Ferrous Hematoxylin shows pale gray-black to no staining. Throw out solution. REFERENCES: * Jim Elsam email to Histonet, October 6, 1997. He credits Pete Jackson at Leeds, England. Stain investigated by Eva Odish, HTL(ASCP), when she was a student in the HTL program at Beaumont Hospital, Royal Oak, MI in 2000. * Source of procedure Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McKnight, Tanisha Sent: Friday, August 08, 2008 1:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Alternatives to Weigert's Iron Hematoxylin for Mucin andTrichrom e Staining Importance: High Hello All: Can anyone suggest an alternative to Weigert's Iron Hematoxylin for nuclei staining with Mucin and Trichrome protocols? I have some standard hematoxylin (7211) that I will be using for my routine staining. However, with the shortage, Weigerts is on backorder until October. Tanisha McKnight, HT(ASCP) AP-Histology/Specimen Management Covance CLS, Indianapolis ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango <@t> gmail.com Fri Aug 8 20:18:48 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Aug 8 20:18:54 2008 Subject: [Histonet] Alternatives to Weigert's Iron Hematoxylin for Mucin and Trichrom e Staining In-Reply-To: <816E3C72F855F14985FC31D7C963AE6F08EC53FB@indexch03.ent.covance.com> References: <816E3C72F855F14985FC31D7C963AE6F08EC53FB@indexch03.ent.covance.com> Message-ID: <5b6eb13e0808081818q4b2c7278i43c7805f9aad3a8e@mail.gmail.com> Hi Tanisha, remember that 7211 hematoxylin is red (but turns blue when blued). You are using Weigert's Iron Hematoxylin because it's grey/black in color and can be seen when there is a lot of color in a stain. When looking for a substitute like this, your best bet will be to stick to the same color. Mark On 8/8/08, McKnight, Tanisha wrote: > > Hello All: > > Can anyone suggest an alternative to Weigert's Iron Hematoxylin for nuclei > staining with Mucin and Trichrome protocols? I have some standard > hematoxylin (7211) that I will be using for my routine staining. However, > with the shortage, Weigerts is on backorder until October. > > > Tanisha McKnight, HT(ASCP) > AP-Histology/Specimen Management > Covance CLS, Indianapolis > > > ----------------------------------------------------- > Confidentiality Notice: This e-mail transmission > may contain confidential or legally privileged > information that is intended only for the individual > or entity named in the e-mail address. If you are not > the intended recipient, you are hereby notified that > any disclosure, copying, distribution, or reliance > upon the contents of this e-mail is strictly prohibited. > > If you have received this e-mail transmission in error, > please reply to the sender, so that we can arrange > for proper delivery, and then please delete the message > from your inbox. Thank you. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tifei <@t> foxmail.com Sat Aug 9 03:15:01 2008 From: tifei <@t> foxmail.com (tf) Date: Sat Aug 9 03:15:20 2008 Subject: [Histonet] HRP reaction on WGA-HRP Message-ID: <200808091614559761336@foxmail.com> Hi All: I am working on tracing with HRP & WGA-HRP I used TMB reaction to visualize the HRP/WGA-HRP. However the staining is a bit uneven, and the blue staining can not be washed down with 0.01 Ace buffer. Do anyone have other good protocols for HRP visualization? or with IHC, anti-WGA, anti-HRP reactions? My detailed protocol as below: 1. wash sections in pure water, 6 times * 5 min 2. A solution + B solution mixture, 20-25 'C, 20 min incubate with sections 3. every 100 ml mixture solution, add 30 ul 30% H2O2, mix well and put the sections back for another 20-25 min 5. wash with 0.01 M Ace buffer , 0-4 'C, 6 times * 5 min 6. mount on to gelatin-coated slides. 2008-08-09 tf From lmayhew5 <@t> cogeco.ca Sat Aug 9 08:52:17 2008 From: lmayhew5 <@t> cogeco.ca (Dave & Lee Mayhew) Date: Sat Aug 9 08:52:19 2008 Subject: [Histonet] Meditech Help Message-ID: <005201c8fa27$22d98b50$e013c143@user4ab93baede> Hi Janelle, We use Meditech and about ten years ago I was on the Implementation Team at my former workplace where I set up the Pathology dictionaries. I'd be happy to help, although I am in Canada, so there will be differences. My best advice would be to have a Histotech involved in setting up the dictionaries....Meditech can be very flexible, you can design it to get a lot of what you want. Lee Mayhew MLT St. Josephs Hospital Hamilton ON Canada 905-522-1155 ex 33276 From dnannenga <@t> incytepathology.com Sat Aug 9 10:01:13 2008 From: dnannenga <@t> incytepathology.com (Debra D. Nannenga) Date: Sat Aug 9 10:01:17 2008 Subject: [Histonet] uneven staining Message-ID: <706224670091FE47997AEF88EFADE7CA09CE05@EXCHANGE-SRV.PAI.E-PATHOLOGY.COM> I am wondering what system you may be using for your staining and also your method for antigen retrieval? We had some similar issues in our lab and we were able to resolve most of them. Debbie Nannenga, HTL,QIHC InCyte Pathology 13103 E. Mansfield Spokane Valley, WA 99216 From dnannenga <@t> incytepathology.com Sat Aug 9 10:06:54 2008 From: dnannenga <@t> incytepathology.com (Debra D. Nannenga) Date: Sat Aug 9 10:06:58 2008 Subject: [Histonet] flitering methods Message-ID: <706224670091FE47997AEF88EFADE7CA09CE06@EXCHANGE-SRV.PAI.E-PATHOLOGY.COM> Hello Histonetters, I was wondering what everyone was using for filtering and processing ECC's, EMC's and the like. We have always used lens papers cut to an appropriate size. We are now having difficulty getting them to even filter, the liquid and tissue just sit on the paper and the liquid does not drain away. I am wondering if this is a new and improved lens paper and that is why it is not working now. Any suggestioons you are willing to share would be appreciated. Debbie Nanennga, HTL,QIHC InCyte Pathology 13103 E. Mansfield Spokane Valley, WA 99216 From joelleweaver <@t> hotmail.com Sat Aug 9 19:49:11 2008 From: joelleweaver <@t> hotmail.com (joelle weaver) Date: Sat Aug 9 19:49:16 2008 Subject: [Histonet] HT schools In-Reply-To: <002c01c8f49a$07995790$e797b348@yourxhtr8hvc4p> References: <78031.12752.qm@web65716.mail.ac4.yahoo.com> <002c01c8f49a$07995790$e797b348@yourxhtr8hvc4p> Message-ID: Take a look at http://www.cscc.edu - see the histology program- under "health careers". This is a distance program with on-line theory, clinical placment possibility (just about anywhere), provisions for working non-certified tech to ASCP certification (via non-traditional credit/competencies) , and also contains a practical component. No techs will graduate who cannot embed or cut. We have more applicants than we could place this time in clinical sites. Thanks> From: jnocito@satx.rr.com> To: rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; amber.mckenzie@gastrodocs.net> Date: Sat, 2 Aug 2008 07:19:36 -0500> Subject: Re: [Histonet] HT schools> CC: > > our one community college program in San Antonio is in jeopardy of closing > because it can't get the required 10 students to make a class. If this > program does close, I will be willing to work with a college or university > with long- distance learning. I have 5 openings in my lab.> > JTT> ----- Original Message ----- > From: "Rene J Buesa" > To: ; "Amber McKenzie" > > Sent: Friday, August 01, 2008 2:42 PM> Subject: Re: [Histonet] HT schools> > > Wrong! The advantage of the "on line" or "distance learning" courses is that > they provide the theory on line while you are working at a given laboratory > doing your training (or even as part of your daily work) so there is no > "actual training" to be done by the supervisor.> At this moment NAACLS has 30 HT and 3 HTL accredited programs only, with an > overall capacity of about 300-325 students, and this will not be enough to > take care of all the retiring histotechs.> Costs is one of the reasons why the number of HTs schools is dwindling.> Ren? J.> > > --- On Fri, 8/1/08, Amber McKenzie wrote:> > From: Amber McKenzie > Subject: [Histonet] HT schools> To: histonet@lists.utsouthwestern.edu> Date: Friday, August 1, 2008, 1:23 PM> > Where are all the HT accredited schools and why aren't there more out> there? I've seen the online classes' people can take, but that> requires> them to be trained in a lab, as well, for the "hands on" part. So,> actually the supervisor still has to train potential HT's "on the> job"> before they can sit for the board exam. Right?> > > > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Your PC, mobile phone, and online services work together like never before. http://clk.atdmt.com/MRT/go/108587394/direct/01/ From renafail <@t> bellsouth.net Sat Aug 9 21:02:27 2008 From: renafail <@t> bellsouth.net (Rena Fail) Date: Sat Aug 9 21:02:45 2008 Subject: [Histonet] Alternatives to Weigert's Iron Hematoxylin for Mucin andTrichrom e Staining In-Reply-To: <816E3C72F855F14985FC31D7C963AE6F08EC53FB@indexch03.ent.covance.com> Message-ID: <001701c8fa8d$24f221c0$0301a8c0@RENAD4YK9B8ABE> You can make your own iron hematoxylins, recipes for Weigert's are in most staining manuals Rena Fail -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McKnight, Tanisha Sent: Friday, August 08, 2008 1:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Alternatives to Weigert's Iron Hematoxylin for Mucin andTrichrom e Staining Importance: High Hello All: Can anyone suggest an alternative to Weigert's Iron Hematoxylin for nuclei staining with Mucin and Trichrome protocols? I have some standard hematoxylin (7211) that I will be using for my routine staining. However, with the shortage, Weigerts is on backorder until October. Tanisha McKnight, HT(ASCP) AP-Histology/Specimen Management Covance CLS, Indianapolis ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lldewe <@t> gmail.com Sun Aug 10 11:45:46 2008 From: lldewe <@t> gmail.com (Loralei Dewe) Date: Sun Aug 10 11:45:53 2008 Subject: [Histonet] Upcoming Webinar Message-ID: <7173d3c00808100945o7e86398eg4d9a2b11169b4bd1@mail.gmail.com> You are invited to attend a live, interactive, web-based tutorial: *==============================================================* Counting, Sorting and Categorizing Objects in Images Presenter Name: Loralei Dewe, Applications Specialist, Media Cybernetics, a MAG Company *==============================================================* Details are below. Connection lines are limited, so reserve yours now. There is no charge to participate in this on-line seminar. *When:* Aug 13, 2008 Wednesday at 10:30 AM Pacific Time *Pre-register (required) at:* http://www.magworldwide.com/education/webinar07150801.php** *Details:* *=====================* Counting objects in images is a task that is routinely performed by scientists in a variety of disciplines. Researchers in material sciences and biology often share the goal of measuring the distribution of particles, cells, and other objects in images based on size, shape, clumpiness, roundness, smoothness, density, color, and other features. Identification and classification of objects in images that contain mixed populations into subgroups based on differences in morphological and optical properties can be tedious, time-consuming, and subject to error unless assisted by image processing tools that are designed for the task. Attendees of this free web-based seminar will learn how to achieve maximum efficiency, accuracy and reproducibility in their analysis by applying a sequence of processing steps to their images and will see how to leverage common feature-extraction and measurement tools to categorize, sort, count, and graph their data. Bring your questions to this live, interactive web-based seminar. Subjects include: - Enhancing images for accurate processing - Performing thresholding operations to separate objects-of-interest from background - Utilizing spatial and morphological filters to differentiate populations of objects - Capturing and presenting your data - Archiving and databasing of images Provided free of charge, this webinar is sponsored by MAG, the Microimaging Applications Group. MAG is a group of imaging companies who work together to provide an unparalleled range of microimaging solutions to science and industry. This seminar requires that attendees use a Java-enabled browser with a high bandwidth connection. Audio is via telephone. Long distance toll charges may apply. There is no charge to participate in this on-line seminar. From Shirley_PHUA <@t> hsa.gov.sg Sun Aug 10 13:08:14 2008 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Sun Aug 10 13:08:31 2008 Subject: [Histonet] Shirley Phua is out-of-office ... Message-ID: I will be out of the office from 11-08-2008 to 13-08-2008. I'll be out-of-singaproe 09-13 August 2008. Pathologists: I will process your requests when I return. If urgent, please forward your email to Henry_Kyaw@hsa.gov.sg From will <@t> histologytechservices.com Sun Aug 10 15:36:25 2008 From: will <@t> histologytechservices.com (William Connor) Date: Sun Aug 10 15:37:14 2008 Subject: [Histonet] uneven ihc staining In-Reply-To: <000001c8f987$59b2a440$d00f7ca5@lurie.northwestern.edu> Message-ID: <0MKpCa-1KSHei3eK0-0005ZD@mrelay.perfora.net> If on an autostainer, it could also be the 'probe', which is what tech support for our autostainer keeps saying...though not likely the case on ours. We are in FL and have noticed for the past couple of years inconsistent staining on DUPLICATE slides has been a problem especially when the temp rises and the humidity increases around June. We are currently in the process of observing this possibility and trying to regulate... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Friday, August 08, 2008 2:48 PM To: rjbuesa@yahoo.com; histonet@lists.utsouthwestern.edu; 'richard shook' Subject: RE: [Histonet] uneven ihc staining Could also be a sensor issue on an autostainer or if the unit has been moved recently and not properly levelled. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, August 08, 2008 9:30 AM To: histonet@lists.utsouthwestern.edu; richard shook Subject: Re: [Histonet] uneven ihc staining Most times this effect is caused by uneven dewaxing of the slides.You should have a very strict protocol for exchanging the dewaxing reagents. Ren? J. --- On Fri, 8/8/08, richard shook wrote: From: richard shook Subject: [Histonet] uneven ihc staining To: histonet@lists.utsouthwestern.edu Date: Friday, August 8, 2008, 8:50 AM Im looking for help we are having uneven staining on some (not all) ihc slides we have looked into several different possoble reasons with no luck ,this happens a couple times every week ao so any suggestion would be great _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Sun Aug 10 21:42:07 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Aug 10 21:42:20 2008 Subject: [Histonet] Alternatives to Weigert's Iron Hematoxylin for Mucin andTrichrom e Staining In-Reply-To: <816E3C72F855F14985FC31D7C963AE6F08EC53FB@indexch03.ent.covance.com> Message-ID: You could try the celestine blue-haematoxylin sequence: This method uses a Modified Harris's haematoxylin but any alum haematoxylin (routinely used in your H&E staining) should work. Document Procedure: The Celestine Blue - Alum Haematoxylin Procedure Principle: Celestine blue resists acid decolourisation and the ferric salt included acts as an additional mordant for the Haematoxylin to provide a reasonably acid resistant nuclear stain. Fixation: 10% buffered formalin. Microtomy: paraffin sections at 5?m. Reagents: 1. Celestine Blue Solution: 0.5% Celestine Blue (CI 51050) in 5% iron alum (ferric ammonium sulphate). 2. Harris's Haematoxylin Solution. Procedure: 1. Deparaffinise and take sections to water. 2. Celestine blue solution, 5 minutes. 3. Rinse in tap water. 4. Harris's Haematoxylin solution, 5 minutes. 5. Wash in water. 6. Differentiate in acid alcohol, as necessary. 7. Wash in water. 8. Blue in blueing solution. 9. Wash in water. 10. Rinse in distilled water. 11. Proceed with required staining technique. Reference: Bancroft, J.D. and Stevens, A. (ed): Theory and Practice of Histological Techniques, first ed. Churchill Livingstone, Edinburgh, 1977, p 88. Henwood, T., Llewellyn, B., Montgomery, I., Nader, A., Rittman, B.R., (2007) "Celestine blue and hemalum for staining nuclei" Biotechnic & Histochem 82(3):167-8. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McKnight, Tanisha Sent: Saturday, 9 August 2008 3:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Alternatives to Weigert's Iron Hematoxylin for Mucin andTrichrom e Staining Importance: High Hello All: Can anyone suggest an alternative to Weigert's Iron Hematoxylin for nuclei staining with Mucin and Trichrome protocols? I have some standard hematoxylin (7211) that I will be using for my routine staining. However, with the shortage, Weigerts is on backorder until October. Tanisha McKnight, HT(ASCP) AP-Histology/Specimen Management Covance CLS, Indianapolis ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From smel0001 <@t> um.edu.mt Mon Aug 11 03:51:03 2008 From: smel0001 <@t> um.edu.mt (STEPHANIE MELI) Date: Mon Aug 11 03:51:28 2008 Subject: [Histonet] Mineral oil in rapid microwave tissue processing Message-ID: <1134.195.158.118.216.1218444663.squirrel@secure.um.edu.mt> Hi to all, I am a student doing my thesis on rapid tissue processing so as to eliminate xylene and reduce processing time. I found some studies which make use of mineral oil as one of the reagents in microwave tissue processing protocols. Can anyone provide me with information regarding its exact use and its effect on the tissue. Thanks a billion. Steph. From XQZhai <@t> uclan.ac.uk Mon Aug 11 04:32:31 2008 From: XQZhai <@t> uclan.ac.uk (Xiao Qun Zhai) Date: Mon Aug 11 04:33:02 2008 Subject: [Histonet] stop subscription Message-ID: <48A01540.4794.0097.0@uclan.ac.uk> Dear Editor, I want to stop my subscription to this net. Thanks very much. Sunny Zhai UK From cmiller <@t> physlab.com Mon Aug 11 05:57:15 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Mon Aug 11 05:58:10 2008 Subject: [Histonet] flitering methods In-Reply-To: <706224670091FE47997AEF88EFADE7CA09CE06@EXCHANGE-SRV.PAI.E-PATHOLOGY.COM> References: <706224670091FE47997AEF88EFADE7CA09CE06@EXCHANGE-SRV.PAI.E-PATHOLOGY.COM> Message-ID: <002601c8fba1$04586ef0$3d02a8c0@plab.local> We filter ours thru a wet blue sponge, place another sponge on top and process as usual. After processing we place the sponges in paraffin and place on the cold plate for a few seconds(using a tamper), pull the sponge off slowly and melt the contents again (pulling out what is trapped in the sponge). This process will capture any tissue present. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debra D. Nannenga Sent: Saturday, August 09, 2008 10:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] flitering methods Hello Histonetters, I was wondering what everyone was using for filtering and processing ECC's, EMC's and the like. We have always used lens papers cut to an appropriate size. We are now having difficulty getting them to even filter, the liquid and tissue just sit on the paper and the liquid does not drain away. I am wondering if this is a new and improved lens paper and that is why it is not working now. Any suggestioons you are willing to share would be appreciated. Debbie Nanennga, HTL,QIHC InCyte Pathology 13103 E. Mansfield Spokane Valley, WA 99216 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From ree3 <@t> leicester.ac.uk Mon Aug 11 06:17:04 2008 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Mon Aug 11 06:18:33 2008 Subject: [Histonet] RE: NOS In-Reply-To: <7722595275A4DD4FA225B92CDBF174A17438DEDB9D@EXC-MBX3.cfs.le.ac.uk> References: <7722595275A4DD4FA225B92CDBF174A17438DEDB9D@EXC-MBX3.cfs.le.ac.uk> Message-ID: <7722595275A4DD4FA225B92CDBF174A17438DEDC04@EXC-MBX3.cfs.le.ac.uk> Anyone experience of immunocytochemistry with the eNOS, iNOS and bNOS antibodies available from Abcam or anywhere else; to be used on cryostat sections of mouse heart. Many thanks Richard Edwards Leicester University...U.K.. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lldewe <@t> gmail.com Mon Aug 11 08:25:34 2008 From: lldewe <@t> gmail.com (Loralei Dewe) Date: Mon Aug 11 08:25:38 2008 Subject: [Histonet] Upcoming webinar, sorry!! Message-ID: <7173d3c00808110625m2e84fbc0weec19bbe62e69e44@mail.gmail.com> Here is the corrected link! My apologies... You are invited to attend a live, interactive, web-based tutorial: *==============================================================* Counting, Sorting and Categorizing Objects in Images Presenter Name: Loralei Dewe, Applications Specialist, Media Cybernetics, a MAG Company *==============================================================* Details are below. Connection lines are limited, so reserve yours now. There is no charge to participate in this on-line seminar. *When:* Aug 13, 2008 Wednesday at 10:30 AM Pacific Time *Pre-register (required) at:* http://www.magworldwide.com/education/webinar07150801.php *Details:* *=====================* Counting objects in images is a task that is routinely performed by scientists in a variety of disciplines. Researchers in material sciences and biology often share the goal of measuring the distribution of particles, cells, and other objects in images based on size, shape, clumpiness, roundness, smoothness, density, color, and other features. Identification and classification of objects in images that contain mixed populations into subgroups based on differences in morphological and optical properties can be tedious, time-consuming, and subject to error unless assisted by image processing tools that are designed for the task. Attendees of this free web-based seminar will learn how to achieve maximum efficiency, accuracy and reproducibility in their analysis by applying a sequence of processing steps to their images and will see how to leverage common feature-extraction and measurement tools to categorize, sort, count, and graph their data. Bring your questions to this live, interactive web-based seminar. Subjects include: - Enhancing images for accurate processing - Performing thresholding operations to separate objects-of-interest from background - Utilizing spatial and morphological filters to differentiate populations of objects - Capturing and presenting your data - Archiving and databasing of images Provided free of charge, this webinar is sponsored by MAG, the Microimaging Applications Group. MAG is a group of imaging companies who work together to provide an unparalleled range of microimaging solutions to science and industry. This seminar requires that attendees use a Java-enabled browser with a high bandwidth connection. Audio is via telephone. Long distance toll charges may apply. There is no charge to participate in this on-line seminar. From rjbuesa <@t> yahoo.com Mon Aug 11 08:31:08 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Aug 11 08:31:12 2008 Subject: [Histonet] Mineral oil in rapid microwave tissue processing In-Reply-To: <1134.195.158.118.216.1218444663.squirrel@secure.um.edu.mt> Message-ID: <881336.1947.qm@web65716.mail.ac4.yahoo.com> Under separate cover I am sending an article I wrote on the subject. Ren? J. --- On Mon, 8/11/08, STEPHANIE MELI wrote: From: STEPHANIE MELI Subject: [Histonet] Mineral oil in rapid microwave tissue processing To: histonet@lists.utsouthwestern.edu Date: Monday, August 11, 2008, 4:51 AM Hi to all, I am a student doing my thesis on rapid tissue processing so as to eliminate xylene and reduce processing time. I found some studies which make use of mineral oil as one of the reagents in microwave tissue processing protocols. Can anyone provide me with information regarding its exact use and its effect on the tissue. Thanks a billion. Steph. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tanisha.mcknight <@t> covance.com Mon Aug 11 08:33:52 2008 From: tanisha.mcknight <@t> covance.com (McKnight, Tanisha) Date: Mon Aug 11 08:34:08 2008 Subject: [Histonet] Dry Storage for Paraffin Blocks and Slides...Recommendations? Message-ID: <816E3C72F855F14985FC31D7C963AE6F08EC59E3@indexch03.ent.covance.com> Good Morning Histonetters: Is there anyone currently using a Desiccator (via Nitrogen) for the dry storage of unstained slides? If so, what kind of system are you currently using and how is it working out for you? Feedback is greatly appreciated. Tanisha McKnight, HT(ASCP) AP-Histology/Specimen Management ----------------------------------------------------- Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From gentras <@t> vetmed.auburn.edu Mon Aug 11 09:20:40 2008 From: gentras <@t> vetmed.auburn.edu (Atoska Gentry) Date: Mon Aug 11 09:20:48 2008 Subject: [Histonet] cryostat block holders In-Reply-To: <611208.37592.qm@web50305.mail.re2.yahoo.com> References: <611208.37592.qm@web50305.mail.re2.yahoo.com> Message-ID: <48A04AB8.3080706@vetmed.auburn.edu> Morning Kim, I believe what you're looking for are the 5ml polystyrene disposable microbeakers. I use Fisher Scientific's # 02-544-30. Best wishes, Atoska Kim Merriam wrote: > Hi everyone, > I want to order some disposable cryostat block holders (the round things that you attach your block to). I don't know what they are officially called, so I am having trouble searching for them. Does anyone know what they are called and if anyone sells plastic ones? > Thanks, > Kim > Kim Merriam, MA, HT(ASCP) > Cambridge, MA > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From WesterM <@t> MedImmune.com Mon Aug 11 09:57:32 2008 From: WesterM <@t> MedImmune.com (Wester, Martha) Date: Mon Aug 11 09:57:48 2008 Subject: [Histonet] research Histotech opportunity Message-ID: <3351384536449948AB336EAE3D7C7420E121DB@MD1EV002.medimmune.com> MedImmune, Inc. has an opportunity for a Pathology Associate in the Research Department at the Gaithersburg facility. The candidate will primarily be responsible for participating in a controlled, GLP environment, performing immunohistochemistry assays and tasks specific to the GxP status. Familiarity with all aspects of the histology lab is desired, especially in immunohistochemistry and cryotomy. The successful candidate will need to be able to adhere to the CFR guidelines, be highly motivated and independent. Bachelor's degree in biological or related science or equivalent experience required. Previous pathology technical experience in academia, pharmaceutical or service industry is desired. Essential skills include good interpersonal skills, word processing abilities, and data management experience. Candidates need possess excellent attention to detail, and an ability to work equally well independently or as part of a project team. Please apply on line at: http://www.medimmune.appone.com/Menu.asp?ClientID=921&B_ID=33&SearchText =pathology+associate&KeyType=all&CatID=0&LocationID=49197&ReqNumber=&x=4 7&y=17 or forward your information to: Martha Wester at westerm@medimmune.com To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From cmiller <@t> physlab.com Mon Aug 11 10:01:33 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Mon Aug 11 10:01:37 2008 Subject: [Histonet] FW: unstained slides Message-ID: <000501c8fbc3$24f687b0$3d02a8c0@plab.local> Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 _____ From: Cheri Miller [mailto:cmiller@physlab.com] Sent: Monday, August 11, 2008 9:17 AM To: histonet-bounces@lists.utsouthwestern.edu Subject: unstained slides How long is everyone keeping their unstained slides (the extra cuts saved for special studies). We hold extra's mostly for our needle biopsies and we file them with the case slides, we are going thru slide cabinets like crazy and I have space issues as it is. Cheri Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From mpence <@t> grhs.net Mon Aug 11 10:06:14 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Mon Aug 11 10:06:19 2008 Subject: [Histonet] FW: unstained slides In-Reply-To: <000501c8fbc3$24f687b0$3d02a8c0@plab.local> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A38CF@IS-E2K3.grhs.net> We save our unstains for 1 month after the case is signed out and then get rid of them because of space issues as well. I can think of maybe twice in 25 plus years I wish I had keep them past 1 month. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Monday, August 11, 2008 10:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: unstained slides Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 _____ From: Cheri Miller [mailto:cmiller@physlab.com] Sent: Monday, August 11, 2008 9:17 AM To: histonet-bounces@lists.utsouthwestern.edu Subject: unstained slides How long is everyone keeping their unstained slides (the extra cuts saved for special studies). We hold extra's mostly for our needle biopsies and we file them with the case slides, we are going thru slide cabinets like crazy and I have space issues as it is. Cheri Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmiller <@t> physlab.com Mon Aug 11 10:19:47 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Mon Aug 11 10:19:49 2008 Subject: [Histonet] Phoenix blue Message-ID: <000601c8fbc5$b1304980$3d02a8c0@plab.local> Anyone using the hematoxilyn substitute; Phoenix blue by Thermo Scientific?? How long are you staining?? Do you still differentiate and blue like regular hematoxilyn? Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From gentras <@t> vetmed.auburn.edu Mon Aug 11 11:09:01 2008 From: gentras <@t> vetmed.auburn.edu (Atoska Gentry) Date: Mon Aug 11 11:09:10 2008 Subject: [Histonet] cryostat block holders In-Reply-To: <001b01c8fbc3$38d8dcb0$6501a8c0@Sunney> References: <611208.37592.qm@web50305.mail.re2.yahoo.com> <48A04AB8.3080706@vetmed.auburn.edu> <001b01c8fbc3$38d8dcb0$6501a8c0@Sunney> Message-ID: <48A0641D.4030402@vetmed.auburn.edu> oops!!! my bad. that number was discontinued the replacement catalog number is: 08-732-119. Hope this works for you. I could not get it to pull up on their website but when I entered it as a rapid order item it came up. Best wishes, Atoska p.s. fisher scientific denotes they're experiencing network problems but online orders can still be placed, perhaps that's the reason. Gayle Callis wrote: > Atoska, > > I tried to bring these up via Thermo Scientific website (a nightmare!) > and found nothing. Are there applications they are used for or some > other such thing? That number didn't work either. > > Ho Hum > > Gayle Callis > > > ----- Original Message ----- From: "Atoska Gentry" > > To: "Kim Merriam" ; "Histonet" > > Sent: Monday, August 11, 2008 8:20 AM > Subject: Re: [Histonet] cryostat block holders > > >> Morning Kim, I believe what you're looking for are the 5ml >> polystyrene disposable microbeakers. I use Fisher Scientific's # >> 02-544-30. Best wishes, Atoska >> >> Kim Merriam wrote: >>> Hi everyone, >>> I want to order some disposable cryostat block holders (the round >>> things that you attach your block to). I don't know what they are >>> officially called, so I am having trouble searching for them. Does >>> anyone know what they are called and if anyone sells plastic ones? >>> Thanks, >>> Kim >>> Kim Merriam, MA, HT(ASCP) >>> Cambridge, MA >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bonnie.Whitaker <@t> osumc.edu Mon Aug 11 11:31:11 2008 From: Bonnie.Whitaker <@t> osumc.edu (Whitaker, Bonnie) Date: Mon Aug 11 11:31:25 2008 Subject: [Histonet] Career ladder/path Message-ID: <3CE20ED86C4A114EBDF3BCE8DEFD8F602625C9@msxc06.OSUMC.EDU> Hi Everyone, I'm trying to come up with a structured career ladder in histology and immunohistochemistry with increasing levels of responsibility and the appropriate increasing levels of compensation. Does anyone have a good model that they would like to share with me? Thanks, Bonnie Whitaker Clinical Histology Manager Ohio State University Medical Center N308B Doan Hall 410 W. 10th Ave. Columbus, OH 43210 Bonnie.Whitaker@osumc.edu phone 614.293.5048 fax 614.293.7273 pager 614.346.5013 From rjbuesa <@t> yahoo.com Mon Aug 11 11:35:21 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Aug 11 11:35:24 2008 Subject: [Histonet] FW: unstained slides In-Reply-To: <000501c8fbc3$24f687b0$3d02a8c0@plab.local> Message-ID: <429965.58522.qm@web65705.mail.ac4.yahoo.com> That depends mostly on the nature of your lab. If you are a regular AP lab then you could dispose of the slides only after the final diagnosis was done. If you are a research institution with residents, you should ask the head of the department to make a decision on the issue after explaining him the space issue. Ren? J. --- On Mon, 8/11/08, Cheri Miller wrote: ? From cbass <@t> wfubmc.edu Mon Aug 11 11:36:20 2008 From: cbass <@t> wfubmc.edu (Caroline Bass) Date: Mon Aug 11 11:36:27 2008 Subject: [Histonet] Sodium nitrite in fixative during perfusion Message-ID: Hello Everyone, Someone recently suggested that I use a 1% sodium nitrite/0.1M phosphate buffer solution during perfusion. I would like to obtain the brain for immunohistochemistry studies. Are there any opinions on whether the sodium nitrite is a good reagent to use for these end points? How does the Na nitrite work, is it a vasodilator? Thanks, Caroline Bass From michelle.steinkrauss <@t> novartis.com Mon Aug 11 12:23:33 2008 From: michelle.steinkrauss <@t> novartis.com (michelle.steinkrauss@novartis.com) Date: Mon Aug 11 12:23:41 2008 Subject: [Histonet] Michelle Steinkrauss is out of the office. Message-ID: I will be out of the office starting 08/11/2008 and will not return until 08/13/2008. If you require immediate assistance for study-related matters, please contact Denise Bland-Piontek at x44146. For clinical pathology assistance, please contact Rhonda Osborne at x 47099. From Beth.Fye <@t> HCAhealthcare.com Mon Aug 11 14:20:02 2008 From: Beth.Fye <@t> HCAhealthcare.com (Fye Beth) Date: Mon Aug 11 14:20:08 2008 Subject: [Histonet] Hospital Pathology - Picking up Specimens Message-ID: <634870CD949E454381124308D16AB619019C7045@NASEV05.hca.corpad.net> I'm curious to how other hospital pathology departments get their OR specimens to Pathology. Do you: 1. Get them delivered to you by OR personnel? 2. Have a lab assistant pick them up? 3. Have histotechs pick them up? 4. Other? Are they carried in Coolers, Biohazard bags, Carts or other method? Do you still use paper requisitions or use some kind of electronic ordering and if you use electronic, what AP system do you have? Thanks in advance for any information you can share! Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 From hyters <@t> onid.orst.edu Mon Aug 11 15:04:02 2008 From: hyters <@t> onid.orst.edu (hyters@onid.orst.edu) Date: Mon Aug 11 15:04:06 2008 Subject: [Histonet] Melanin bleaching and IHC-P Message-ID: <20080811130402.zlf2kqqlnkgskk0s@webmail.oregonstate.edu> Hello, I am attempting to perform IHC-P on heavily pigmented melanocytic growths using flourescent antibodies. I have tried using 10% H2O2 both overnite at room temperature and over two hours at 60 degrees. The bleaching is decent but tissue integrity suffers greatly. I have not tried the potassium permanganate/oxalic acid method yet (although at this point I will have to try it) but I had a question about the necessity of bleaching as I am not using chromagen-based antibodies. The bleaching does not remove the pigment (correct?) so it is not a question of primary antibody accessibility but of color differentiation right? Since I am using flouresence microscopy is bleaching the growths a necessary step? Thanks for your time, Stephen From marktarango <@t> gmail.com Mon Aug 11 15:11:44 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Mon Aug 11 15:11:50 2008 Subject: [Histonet] Melanin bleaching and IHC-P In-Reply-To: <20080811130402.zlf2kqqlnkgskk0s@webmail.oregonstate.edu> References: <20080811130402.zlf2kqqlnkgskk0s@webmail.oregonstate.edu> Message-ID: <5b6eb13e0808111311r2a65e986v310a42e8c6a8a9a9@mail.gmail.com> Hi Stephan, Yep, you don't have to bleach it because this is immunofloresece. People bleach because melanin pigment can look a lot like the chromogen DAB. I think it's better to just do it in red, if it were light microscopy though... Mark On 8/11/08, hyters@onid.orst.edu wrote: > > Hello, > > I am attempting to perform IHC-P on heavily pigmented melanocytic growths > using flourescent antibodies. I have tried using 10% H2O2 both overnite at > room temperature and over two hours at 60 degrees. The bleaching is decent > but tissue integrity suffers greatly. > > I have not tried the potassium permanganate/oxalic acid method yet > (although at this point I will have to try it) but I had a question about > the necessity of bleaching as I am not using chromagen-based antibodies. > The bleaching does not remove the pigment (correct?) so it is not a > question of primary antibody accessibility but of color differentiation > right? Since I am using flouresence microscopy is bleaching the growths a > necessary step? > > Thanks for your time, > Stephen > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Mon Aug 11 15:37:52 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Aug 11 15:37:56 2008 Subject: [Histonet] Hospital Pathology - Picking up Specimens In-Reply-To: <634870CD949E454381124308D16AB619019C7045@NASEV05.hca.corpad.net> Message-ID: <122959.57859.qm@web65705.mail.ac4.yahoo.com> #s 1 and 2 Ren? J. --- On Mon, 8/11/08, Fye Beth wrote: From: Fye Beth Subject: [Histonet] Hospital Pathology - Picking up Specimens To: histonet@lists.utsouthwestern.edu Date: Monday, August 11, 2008, 3:20 PM I'm curious to how other hospital pathology departments get their OR specimens to Pathology. Do you: 1. Get them delivered to you by OR personnel? 2. Have a lab assistant pick them up? 3. Have histotechs pick them up? 4. Other? Are they carried in Coolers, Biohazard bags, Carts or other method? Do you still use paper requisitions or use some kind of electronic ordering and if you use electronic, what AP system do you have? Thanks in advance for any information you can share! Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amber.mckenzie <@t> gastrodocs.net Mon Aug 11 15:48:08 2008 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Mon Aug 11 15:48:12 2008 Subject: [Histonet] Hospital Pathology - Picking up Specimens In-Reply-To: <122959.57859.qm@web65705.mail.ac4.yahoo.com> Message-ID: <03C921A1EAF7F541B16543F6EC6A4B3701D8E2F3@giamail2.Gia.com> Beth, We have a lab assistant pick up our specimens in a cooler type bag that we bought from Wal-Mart labeled with biohazard stickers. We still use paper requisitions. Amber -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, August 11, 2008 3:38 PM To: histonet@lists.utsouthwestern.edu; Fye Beth Subject: Re: [Histonet] Hospital Pathology - Picking up Specimens #s 1 and 2 Ren? J. --- On Mon, 8/11/08, Fye Beth wrote: From: Fye Beth Subject: [Histonet] Hospital Pathology - Picking up Specimens To: histonet@lists.utsouthwestern.edu Date: Monday, August 11, 2008, 3:20 PM I'm curious to how other hospital pathology departments get their OR specimens to Pathology. Do you: 1. Get them delivered to you by OR personnel? 2. Have a lab assistant pick them up? 3. Have histotechs pick them up? 4. Other? Are they carried in Coolers, Biohazard bags, Carts or other method? Do you still use paper requisitions or use some kind of electronic ordering and if you use electronic, what AP system do you have? Thanks in advance for any information you can share! Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hlee <@t> medsci.usyd.edu.au Mon Aug 11 18:49:33 2008 From: hlee <@t> medsci.usyd.edu.au (Hyunchul Lee) Date: Mon Aug 11 18:49:39 2008 Subject: [Histonet] Treating Golgi-Kopsch sections with photographic developers Message-ID: <495381.82487.qm@web31404.mail.mud.yahoo.com> Hi, I'm trying to stabilize Golgi-Kopsch sections with photographic developers, but the sections turn very black almost instantly after being immersed in developer. The sections become opaque and upon fixing in Kodak rapid fixer, will gradually become clearer over the course of hours (rather than minutes). Anyone else have any other ideas on how to make this work, or what my problem is? Thanks Hyunchul Lee PhD student Systems Neuroscience Laboratory N121 Anderson Stuart Bldg. (F13) The University of Sydney, NSW, 2006 AUSTRALIA Email: hlee@medsci.usyd.edu.au From brucea <@t> unimelb.edu.au Mon Aug 11 23:57:52 2008 From: brucea <@t> unimelb.edu.au (Bruce Abaloz) Date: Mon Aug 11 23:58:03 2008 Subject: [Histonet] filtering methods Message-ID: Hi Debra, We use 'Biopsy pads' which are a blue sponge material (also come in green but we find blue shows the tiniest bits best) that will hold the tiniest of tissue Bx's (you can also 'colour' your specimen with Eosin so it can be seen easier). Be SURE to source a sponge/pad, that has a very fine sponge as some we trialled were quite 'porous'/allowed tiny bits to move into the sponge. Place tissue on bottom layer then cover with another pad & close your cassette. Dip into 70% ETOH before you drop into formalin/fixative as sponges are 'hydrophobic' until they have been wetted with ETOH. Process as usual, remove from paraffin, open cassette & 'voila' - there is the Bx.- safe'n'sound! -- BRUCE ABALOZ Histology Facility Manager The University of Melbourne Department of Zoology Victoria. AUSTRALIA 3010 Ph: (03) 83446282 Fax:(03) 83447909 Email: brucea@unimelb.edu.au Please BCC my email address in ALL group emails for privacy and spam issues. Thank you. Nobody Can Make You Feel Inferior Without YOUR Permission - Eleanor Roosevelt WHETHER YOU THINK YOU CAN-OR WHETHER YOU THINK YOU CAN'T - YOU'RE RIGHT. P Please consider the environment before printing this email note. This email and any attachments may contain personal information or information that is otherwise privileged, confidential or otherwise protected from disclosure/subject of copyright. Any use, disclosure or copying of any part of it is prohibited. The University does not warrant that this email or any attachments are free from viruses or defects. Please check any attachments for viruses and defects before opening them. If this email is received in error please delete it and notify us by return email. The University does not necessarily share or endorse the views expressed in this email. From sheila_adey <@t> hotmail.com Tue Aug 12 05:14:47 2008 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Tue Aug 12 05:14:52 2008 Subject: [Histonet] Had our CAP inspection yesterday Message-ID: Hi all, The unexpected show up was yesterday for us. We were for sure surprised to see them show up on a Monday. Couldn't have picked a busier day of the week? Anyway, I just wanted to share some of their "interpretations" of the checklist. Storing your blocks and slides at room temp? You need to prove it is room temp and monitored. We will now have thermometers in these locations. One of our paths removed all the sign off areas from the individual policies and had a sign off list at the front of the book. They didn't like that either. Glad to have it over with. Sheila Adey HT MLTPort Huron HospitalMichigan _________________________________________________________________ From sheila_adey <@t> hotmail.com Tue Aug 12 05:16:27 2008 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Tue Aug 12 05:16:30 2008 Subject: [Histonet] Hospital Pathology - Picking up Specimens In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B3701D8E2F3@giamail2.Gia.com> References: <122959.57859.qm@web65705.mail.ac4.yahoo.com> <03C921A1EAF7F541B16543F6EC6A4B3701D8E2F3@giamail2.Gia.com> Message-ID: Just this year we started having the OR aids drop them off. They could do it after hours. Now we don't know where we found the time to run all over the hospital picking up specimens.Sheila Adey HT MLTPort Huron HospitalMichigan> Date: Mon, 11 Aug 2008 15:48:08 -0500> From: amber.mckenzie@gastrodocs.net> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] Hospital Pathology - Picking up Specimens> > Beth,> > We have a lab assistant pick up our specimens in a cooler type bag that we bought from Wal-Mart labeled with biohazard stickers. We still use paper requisitions.> > Amber> > -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa> Sent: Monday, August 11, 2008 3:38 PM> To: histonet@lists.utsouthwestern.edu; Fye Beth> Subject: Re: [Histonet] Hospital Pathology - Picking up Specimens> > #s 1 and 2> Ren? J.> > --- On Mon, 8/11/08, Fye Beth wrote:> > From: Fye Beth > Subject: [Histonet] Hospital Pathology - Picking up Specimens> To: histonet@lists.utsouthwestern.edu> Date: Monday, August 11, 2008, 3:20 PM> > I'm curious to how other hospital pathology departments get their OR> specimens to Pathology.> Do you:> 1. Get them delivered to you by OR personnel?> 2. Have a lab assistant pick them up?> 3. Have histotechs pick them up?> 4. Other?> > Are they carried in Coolers, Biohazard bags, Carts or other method?> > Do you still use paper requisitions or use some kind of electronic> ordering and if you use electronic, what AP system do you have?> > Thanks in advance for any information you can share!> > > Beth A. Fye, CT (ASCP)> Pathology Technical Manager> HCA Richmond Hospital Laboratories> office: (804)228-6564> > > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ If you like crossword puzzles, then you'll love Flexicon, a game which combines four overlapping crossword puzzles into one! http://g.msn.ca/ca55/208 From GauchV <@t> mail.amc.edu Tue Aug 12 07:33:26 2008 From: GauchV <@t> mail.amc.edu (Vicki Gauch) Date: Tue Aug 12 07:34:12 2008 Subject: [Histonet] Hospital Pathology - Picking up Specimens Message-ID: We have our frozen section room in the OR so the OR staff delivers all OR specimens to the frozen section room where it is triaged...involved cases are grossed in the FS room , smaller cases are picked up by our Lab Info Techs on scheduled runs throughout the day. We use carts which we have to cover when transporting through the hospital. We still use paper requisitions but the hospital is looking into electronic ordering now. Hope that helps, Vicki AMCH Albany, NY >>> "Fye Beth" 8/11/2008 3:20 PM >>> I'm curious to how other hospital pathology departments get their OR specimens to Pathology. Do you: 1. Get them delivered to you by OR personnel? 2. Have a lab assistant pick them up? 3. Have histotechs pick them up? 4. Other? Are they carried in Coolers, Biohazard bags, Carts or other method? Do you still use paper requisitions or use some kind of electronic ordering and if you use electronic, what AP system do you have? Thanks in advance for any information you can share! Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. From HornHV <@t> archildrens.org Tue Aug 12 07:48:32 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Aug 12 07:50:03 2008 Subject: [Histonet] Hospital Pathology - Picking up Specimens In-Reply-To: <634870CD949E454381124308D16AB619019C7045@NASEV05.hca.corpad.net> References: <634870CD949E454381124308D16AB619019C7045@NASEV05.hca.corpad.net> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82D7C@EMAIL.archildrens.org> Our OR delivers the specimens to our lab. They are in plastic bags and arrive with a paper requisition. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fye Beth Sent: Monday, August 11, 2008 2:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hospital Pathology - Picking up Specimens I'm curious to how other hospital pathology departments get their OR specimens to Pathology. Do you: 1. Get them delivered to you by OR personnel? 2. Have a lab assistant pick them up? 3. Have histotechs pick them up? 4. Other? Are they carried in Coolers, Biohazard bags, Carts or other method? Do you still use paper requisitions or use some kind of electronic ordering and if you use electronic, what AP system do you have? Thanks in advance for any information you can share! Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From mpence <@t> grhs.net Tue Aug 12 08:04:44 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Aug 12 08:04:49 2008 Subject: [Histonet] Had our CAP inspection yesterday In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A38D0@IS-E2K3.grhs.net> What is room temp.? Isn't room temp. the temperature of a given room? Room temp. in a furnace room is not room temp. in a "cold" room. Is it Friday yet? Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sheila adey Sent: Tuesday, August 12, 2008 5:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Had our CAP inspection yesterday Hi all, The unexpected show up was yesterday for us. We were for sure surprised to see them show up on a Monday. Couldn't have picked a busier day of the week? Anyway, I just wanted to share some of their "interpretations" of the checklist. Storing your blocks and slides at room temp? You need to prove it is room temp and monitored. We will now have thermometers in these locations. One of our paths removed all the sign off areas from the individual policies and had a sign off list at the front of the book. They didn't like that either. Glad to have it over with. Sheila Adey HT MLTPort Huron HospitalMichigan _________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mwich <@t> 7thwavelabs.com Tue Aug 12 08:36:41 2008 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Tue Aug 12 08:36:48 2008 Subject: [Histonet] equipment preferences Message-ID: <62A8156F8071C8439080D626DF8C33A602E471@wave-mail.7thwave.local> We are thinking about purchasing a new slide stainer (H&E) and embedding center. Any suggestions/recommendations/vendor preferences would be greatly appreciated. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From minhan.tan <@t> gmail.com Tue Aug 12 09:09:14 2008 From: minhan.tan <@t> gmail.com (Min-Han Tan) Date: Tue Aug 12 09:23:30 2008 Subject: [Histonet] ERCC1 In-Reply-To: <466878.15435.qm@web61212.mail.yahoo.com> References: <466878.15435.qm@web61212.mail.yahoo.com> Message-ID: Hello all ye experts, May I find out which anti-ERCC1 antibody (as well as which clone) are you using in your clinical laboratory at the moment? Thank you. I am looking around for a good antibody for paraffin- embedded tissue. Regards, Min-Han Tan From trathborne <@t> somerset-healthcare.com Tue Aug 12 09:33:19 2008 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Tue Aug 12 09:33:29 2008 Subject: [Histonet] equipment preferences In-Reply-To: <62A8156F8071C8439080D626DF8C33A602E471@wave-mail.7thwave.local> Message-ID: We love our Leica ST5020. It can be used with or without coverslipper CV5030. Toni Rathborne Pathology Supervisor Somerset Medical Center 110 Rehill Ave. Somerville,NJ 08876 908-595-2367 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Michele Wich Sent: Tuesday, August 12, 2008 9:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] equipment preferences We are thinking about purchasing a new slide stainer (H&E) and embedding center. Any suggestions/recommendations/vendor preferences would be greatly appreciated. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From SohrabB1 <@t> ah.org Tue Aug 12 10:33:19 2008 From: SohrabB1 <@t> ah.org (Behnaz Sohrab) Date: Tue Aug 12 10:33:57 2008 Subject: [Histonet] Job opening Message-ID: <48A14ACE.4347.0054.0@ah.org> Hi Dear Hitotologists We have histo-tech position open at White Memorial Med. ctr. in Los Angeles area, Monday - Friday 4 Am - 12.30 PM. If you know of any tech contact me @ 323-268-5000 EXT.11711#. Thanks,Behnaz Sohrab From Janice.Fightmaster <@t> HCAhealthcare.com Tue Aug 12 10:36:01 2008 From: Janice.Fightmaster <@t> HCAhealthcare.com (Fightmaster Janice) Date: Tue Aug 12 10:36:06 2008 Subject: [Histonet] Hematoxylin shortage Message-ID: What is the latest on the hematoxylin shortage? We have not received any stain we have ordered for the last few months. I am down to 3 small bottles of Gill I. Has anyone tried any of the synthetics, and if so, which ones? How are they working for you? Any and all help or suggestions would be appreciated. I am having difficulty even getting a sample of a synthetic! Thanks so much. Janice Fightmaster CT(ASCP)HT Oak Hill Hospital 11375 Cortez Blvd. Brooksville, FL 34613 (352)597-6363 Ext. 3636 From rjbuesa <@t> yahoo.com Tue Aug 12 10:37:40 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Aug 12 10:37:47 2008 Subject: [Histonet] equipment preferences In-Reply-To: <62A8156F8071C8439080D626DF8C33A602E471@wave-mail.7thwave.local> Message-ID: <522571.36695.qm@web65710.mail.ac4.yahoo.com> Buy Sakura. Ren? J. --- On Tue, 8/12/08, Michele Wich wrote: From: Michele Wich Subject: [Histonet] equipment preferences To: histonet@lists.utsouthwestern.edu Date: Tuesday, August 12, 2008, 9:36 AM We are thinking about purchasing a new slide stainer (H&E) and embedding center. Any suggestions/recommendations/vendor preferences would be greatly appreciated. This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From margaret <@t> imebinc.com Tue Aug 12 11:32:49 2008 From: margaret <@t> imebinc.com (Margaret O'Donnell) Date: Tue Aug 12 11:29:29 2008 Subject: [Histonet] Hematoxylin shortage In-Reply-To: Message-ID: <20080812092718.939F3AB3@dm02.mta.everyone.net> I'm with IMEB INC, we specialize in pathology equipment and lab supplies. We have plenty of hematoxylin! See this link to our website: http://www.imebinc.com/Item/HEMATOX.htm You may order on-line, or call our toll free number: 800-543-8496, or fax orders to: 760-761-0859. Margaret O'Donnell Sales Rep IMEB, INC. 800-543-8496 760-761-0859 fax "The Pathology Specialists" margaret@imebinc.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fightmaster Janice Sent: Tuesday, August 12, 2008 8:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hematoxylin shortage What is the latest on the hematoxylin shortage? We have not received any stain we have ordered for the last few months. I am down to 3 small bottles of Gill I. Has anyone tried any of the synthetics, and if so, which ones? How are they working for you? Any and all help or suggestions would be appreciated. I am having difficulty even getting a sample of a synthetic! Thanks so much. Janice Fightmaster CT(ASCP)HT Oak Hill Hospital 11375 Cortez Blvd. Brooksville, FL 34613 (352)597-6363 Ext. 3636 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From treese <@t> sleh.com Tue Aug 12 13:14:34 2008 From: treese <@t> sleh.com (Reese, Tommy G.) Date: Tue Aug 12 13:14:41 2008 Subject: [Histonet] Aqueous mounting medium Message-ID: <023DBFA505E67A4689A2CEEC40AE3F7D8E4CA09B85@SLEHEXCH02.sleh.com> Several people have inquired over the last few weeks/months about substitutes for Biomeda mounting medium. Our lab has used Diagnostic Biosystems CC/Mount and Fluoromount/Plus and have had excellent results. +++++CONFIDENTIALITY NOTICE+++++ The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. From Kimberly.Marshall <@t> ahss.org Tue Aug 12 13:43:13 2008 From: Kimberly.Marshall <@t> ahss.org (Marshall, Kimberly) Date: Tue Aug 12 13:43:22 2008 Subject: [Histonet] RE:Hospital Pathology-Picking up specimen Message-ID: Howdy All We take a bucket and pick our specimens up. In our policy it is the O.R. nurses that are supposed to get them to us. But if we did that we would get everything at 3 P.M. in the afternoon, so we get them, just easier than waiting. Kimberly ============================================================================== The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ============================================================================== From mike <@t> pathview.com Tue Aug 12 13:57:45 2008 From: mike <@t> pathview.com (Michael Mihalik) Date: Tue Aug 12 13:59:22 2008 Subject: [Histonet] Hospital Pathology - Picking up Specimens In-Reply-To: References: Message-ID: <03a701c8fcad$6a355140$3e9ff3c0$@com> Just a note of caution for those of you looking at electronic ordering. No matter whose system you employ, you'll want to have the capability to edit the orders that come down from the floor. We're big proponents of electronic orders, heck electronic everything, but surgical orders from the floor have a high degree of error associated with them. Because of that, you'll want to be able to edit those orders as they are entered into your AP system. The good news with electronic ordering is that patient demographic and billing information is generally correct AND you have the potential to have a better idea of exactly how many specimen to expect for a given case. Obviously, this information leads to the reduction of a lot of errors and wasted departmental time. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vicki Gauch Sent: Tuesday, August 12, 2008 8:33 AM To: Fye Beth; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Hospital Pathology - Picking up Specimens We have our frozen section room in the OR so the OR staff delivers all OR specimens to the frozen section room where it is triaged...involved cases are grossed in the FS room , smaller cases are picked up by our Lab Info Techs on scheduled runs throughout the day. We use carts which we have to cover when transporting through the hospital. We still use paper requisitions but the hospital is looking into electronic ordering now. Hope that helps, Vicki AMCH Albany, NY >>> "Fye Beth" 8/11/2008 3:20 PM >>> I'm curious to how other hospital pathology departments get their OR specimens to Pathology. Do you: 1. Get them delivered to you by OR personnel? 2. Have a lab assistant pick them up? 3. Have histotechs pick them up? 4. Other? Are they carried in Coolers, Biohazard bags, Carts or other method? Do you still use paper requisitions or use some kind of electronic ordering and if you use electronic, what AP system do you have? Thanks in advance for any information you can share! Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LRaff <@t> uropartners.com Tue Aug 12 13:59:48 2008 From: LRaff <@t> uropartners.com (Lester Raff MD) Date: Tue Aug 12 13:59:54 2008 Subject: [Histonet] RE:Hospital Pathology-Picking up specimen In-Reply-To: Message-ID: A word of caution. Make sure you have a means of documenting what specimens are in the OR specimen holding area, who placed them there, who picked them up and at what time. Otherwise, the lab could be held responsible if a specimen "disappears". Yes, I have seen it happen..... Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.486.0080 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall, Kimberly Sent: Tuesday, August 12, 2008 1:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE:Hospital Pathology-Picking up specimen Howdy All We take a bucket and pick our specimens up. In our policy it is the O.R. nurses that are supposed to get them to us. But if we did that we would get everything at 3 P.M. in the afternoon, so we get them, just easier than waiting. Kimberly ======================================================================== ====== The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cbass <@t> wfubmc.edu Tue Aug 12 14:18:25 2008 From: cbass <@t> wfubmc.edu (Caroline Bass) Date: Tue Aug 12 14:18:33 2008 Subject: [Histonet] Is iba-1 a good marker for microglia in rats? Message-ID: Hey guys, I asked a few days ago for antigens to distinguish microglia via IHC in rats. I had numerous suggestions, but discovered that I have an antibody for Iba-1 in the lab. Any recommendations to how this might work in the rat and if it?s a good antigen for microglia? Thanks, Caroline Bass From HornHV <@t> archildrens.org Tue Aug 12 14:36:40 2008 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Aug 12 14:36:59 2008 Subject: [Histonet] RE:Hospital Pathology-Picking up specimen In-Reply-To: References: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C6317D82D85@EMAIL.archildrens.org> Yes we do that...The OR has a log that they record the specimens on and when they bring them to us we sign off on them... Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lester Raff MD Sent: Tuesday, August 12, 2008 2:00 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE:Hospital Pathology-Picking up specimen A word of caution. Make sure you have a means of documenting what specimens are in the OR specimen holding area, who placed them there, who picked them up and at what time. Otherwise, the lab could be held responsible if a specimen "disappears". Yes, I have seen it happen..... Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.486.0080 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall, Kimberly Sent: Tuesday, August 12, 2008 1:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE:Hospital Pathology-Picking up specimen Howdy All We take a bucket and pick our specimens up. In our policy it is the O.R. nurses that are supposed to get them to us. But if we did that we would get everything at 3 P.M. in the afternoon, so we get them, just easier than waiting. Kimberly ======================================================================== ====== The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From jblaine <@t> bio-search.com Tue Aug 12 15:55:09 2008 From: jblaine <@t> bio-search.com (Jason Blaine) Date: Tue Aug 12 15:55:15 2008 Subject: [Histonet] Histotech Position @ NIH / NEI - Career Opportunity (Bethesda, MD) In-Reply-To: <038801c8fcb4$2eb62160$046fa8c0@Team.teamplace.com> References: <038801c8fcb4$2eb62160$046fa8c0@Team.teamplace.com> Message-ID: <000301c8fcbd$b5ceda30$046fa8c0@Team.teamplace.com> Position Overview: The Histotechnician position is in the Core Histology Lab within the National Eye Institute (NEI) at NIH. Because all of the cases are research based this a low volume laboratory with an emphasis on high quality. They are willing to train on working with the eye but need someone with basic Histology skills. Details: Days: Monday - Friday (no holidays) Hours: 8:30AM - 5:00PM (flexible within an hour either direction) Location: NIH Main Campus (Bethesda, MD 20892) Status: Fulltime, benefited (insurance, sick leave, vacation leave, holiday pay, etc.) contractor Compensation: competitive Requirements: * Routine histology functions (i.e. embedding, cutting, special stains and frozen sections). * Basic proficiency in IHC (manual) is preferred. * Previous experience with the eye is highly desired but not required. * HT or HTL eligibility/certification a plus. Qualified candidate please send resume/CV to jblaine@bio-search.com Thanks - Jason Blaine Director Tel: 301-565-3908 ext. 301 Cell: 301-219-3379 Fax: 301-565-3915 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ BioSearch a division of Team Placement Service, Inc. From mpence <@t> grhs.net Tue Aug 12 16:40:44 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Aug 12 16:40:50 2008 Subject: [Histonet] RE:Hospital Pathology-Picking up specimen In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A38D1@IS-E2K3.grhs.net> Then why have a policy? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall, Kimberly Sent: Tuesday, August 12, 2008 1:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE:Hospital Pathology-Picking up specimen Howdy All We take a bucket and pick our specimens up. In our policy it is the O.R. nurses that are supposed to get them to us. But if we did that we would get everything at 3 P.M. in the afternoon, so we get them, just easier than waiting. Kimberly ======================================================================== ====== The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Tue Aug 12 17:00:39 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Aug 12 17:00:52 2008 Subject: [Histonet] ERCC1 In-Reply-To: Message-ID: Here is source and working conditions for ERCC1: Clone: 8F1 Isotype: IgG2b DESCRIPTION: Monoclonal antibody to Recombinant full length human ERCC1 protein. STORAGE CONDITIONS: 4-8oC undiluted SUPPLIER: Neomarkers MS-671-P0 PROCEDURE: Methodology: HRP-POLYMER Working Dilution: Bond: 1/400 (ER1) Special Conditions: Citrate Heat induced epitope retrieval required CONTROL TISSUE: Tonsil Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Min-Han Tan Sent: Wednesday, 13 August 2008 12:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ERCC1 Hello all ye experts, May I find out which anti-ERCC1 antibody (as well as which clone) are you using in your clinical laboratory at the moment? Thank you. I am looking around for a good antibody for paraffin- embedded tissue. Regards, Min-Han Tan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From alzcarlos <@t> hotmail.com Tue Aug 12 15:21:18 2008 From: alzcarlos <@t> hotmail.com (Carlos Augusto) Date: Tue Aug 12 19:03:27 2008 Subject: [Histonet] Muscle striations on paraffin - Sarcomeres Message-ID: Dear histonetters Here at the lab a university doctor have asked to do some muscle histology of scallop and oyster species. She asked if the striations could be looked in a Masson?s Trichrome stain. She intends to measure the sarcomeres (the spaces between two z-lines) in the mollusc?s muscles, but I have had a lot of problems when the sections are stained. 1- In other species the striations are clearly visible (rabit, fish and chicken slides from the student?s histology couse were stained at the same time), but not in the molluscs. 2- Cells (muscle fibers) are very thin and even in 4 micrometer sections the fibers one over the others. So the microscopy is very "disrty" to look at. 3- We changed the stain for the Millingan?s Trichrome but only in one or two slides could be seen some signs of striation. The tissues are fixed in 10% Neutral Buffered Formalin, washed, dehydrated in ethylic alcohols, cleared on hemo-de (xylol substitute-limonenes), infiltrated and embbeded with paraplast. Sections were done on a rotary microtome with disposable blades. Any other way to look at the sarcomeres on paraffin processed tissues are very VERY appreciated. Is there one way to know if there is a stain to differentiate sriated from smooth muscle cells? Thanks in advance. Carlos Aguilar Cruz Laboratorio de Histolog?a - Unidad Pichilingue Universidad Aut?noma de Baja California Sur. Carretera al Sur KM 5.5 CP230080 La Paz, BCS, M?xico From AnthonyH <@t> chw.edu.au Tue Aug 12 19:13:20 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Aug 12 19:13:31 2008 Subject: [Histonet] Muscle striations on paraffin - Sarcomeres In-Reply-To: Message-ID: Have you tried a PTAH stain? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carlos Augusto Sent: Wednesday, 13 August 2008 6:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Muscle striations on paraffin - Sarcomeres Dear histonetters Here at the lab a university doctor have asked to do some muscle histology of scallop and oyster species. She asked if the striations could be looked in a Masson?s Trichrome stain. She intends to measure the sarcomeres (the spaces between two z-lines) in the mollusc?s muscles, but I have had a lot of problems when the sections are stained. 1- In other species the striations are clearly visible (rabit, fish and chicken slides from the student?s histology couse were stained at the same time), but not in the molluscs. 2- Cells (muscle fibers) are very thin and even in 4 micrometer sections the fibers one over the others. So the microscopy is very "disrty" to look at. 3- We changed the stain for the Millingan?s Trichrome but only in one or two slides could be seen some signs of striation. The tissues are fixed in 10% Neutral Buffered Formalin, washed, dehydrated in ethylic alcohols, cleared on hemo-de (xylol substitute-limonenes), infiltrated and embbeded with paraplast. Sections were done on a rotary microtome with disposable blades. Any other way to look at the sarcomeres on paraffin processed tissues are very VERY appreciated. Is there one way to know if there is a stain to differentiate sriated from smooth muscle cells? Thanks in advance. Carlos Aguilar Cruz Laboratorio de Histolog?a - Unidad Pichilingue Universidad Aut?noma de Baja California Sur. Carretera al Sur KM 5.5 CP230080 La Paz, BCS, M?xico ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From minhan.tan <@t> gmail.com Tue Aug 12 20:02:02 2008 From: minhan.tan <@t> gmail.com (Min-Han Tan) Date: Tue Aug 12 20:02:07 2008 Subject: [Histonet] ERCC1 In-Reply-To: References: Message-ID: <920cc4a70808121802j2316bf25m7efb34a90dda5412@mail.gmail.com> Thank you all for your information. It has been very helpful! Regards, Min-Han Tan National Cancer Centre Singapore On Wed, Aug 13, 2008 at 6:00 AM, Tony Henwood wrote: > Here is source and working conditions for ERCC1: > Clone: 8F1 > Isotype: IgG2b > > > DESCRIPTION: Monoclonal antibody to Recombinant full length human > ERCC1 protein. > > STORAGE CONDITIONS: 4-8oC undiluted > > SUPPLIER: Neomarkers MS-671-P0 > PROCEDURE: > > Methodology: HRP-POLYMER Working Dilution: Bond: > 1/400 (ER1) > Special Conditions: Citrate Heat induced epitope retrieval required > > CONTROL TISSUE: Tonsil > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Min-Han > Tan > Sent: Wednesday, 13 August 2008 12:09 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] ERCC1 > > > Hello all ye experts, > > May I find out which anti-ERCC1 antibody (as well as which clone) are > you using in your clinical laboratory at the moment? > > Thank you. I am looking around for a good antibody for paraffin- > embedded tissue. > > Regards, > Min-Han Tan > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead > > This note also confirms that this email message has been > virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************** > > From cmiller <@t> physlab.com Wed Aug 13 07:56:18 2008 From: cmiller <@t> physlab.com (Cheri Miller) Date: Wed Aug 13 07:56:29 2008 Subject: [Histonet] Aspira CyberPATH Message-ID: <000501c8fd43$faf167a0$3d02a8c0@plab.local> Is anyone in Histoland using Aspira -CyberPATH for their Anatomic computer system?? If so could you please email me with your thoughts, suggestions or advice? Also I am curious about the billing part. We have several Questions regarding CPT codes etc. Thanks Cheri Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From Tiffany.Price <@t> thomaswv.org Wed Aug 13 08:51:09 2008 From: Tiffany.Price <@t> thomaswv.org (Price, Tiffany) Date: Wed Aug 13 08:51:22 2008 Subject: [Histonet] re-staining an IHC slide for H&E Message-ID: <7B5F5D9A00739F43A1819403EC7CF49108AA2527@thm-mail.thomaswv.org> I have been asked to take a negative control slide and re-stain it for H&E. Does anyone have a protocol for this? Thanks Confidentiality Notice: This email message including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privilege information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy ALL copies of the message. From rjbuesa <@t> yahoo.com Wed Aug 13 10:26:58 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Aug 13 10:27:01 2008 Subject: [Histonet] re-staining an IHC slide for H&E In-Reply-To: <7B5F5D9A00739F43A1819403EC7CF49108AA2527@thm-mail.thomaswv.org> Message-ID: <306662.25795.qm@web65712.mail.ac4.yahoo.com> Just take the coverslip off, hydrate and stain as usual. Ren? J. --- On Wed, 8/13/08, Price, Tiffany wrote: From: Price, Tiffany Subject: [Histonet] re-staining an IHC slide for H&E To: histonet@lists.utsouthwestern.edu Date: Wednesday, August 13, 2008, 9:51 AM I have been asked to take a negative control slide and re-stain it for H&E. Does anyone have a protocol for this? Thanks Confidentiality Notice: This email message including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privilege information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy ALL copies of the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Wed Aug 13 10:53:23 2008 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Wed Aug 13 10:53:38 2008 Subject: [Histonet] re-staining an IHC slide for H&E In-Reply-To: <306662.25795.qm@web65712.mail.ac4.yahoo.com> Message-ID: Tiffany, I would suggest doubling the Hematoxilyn time if the negative went through an enzyme retrieval as this would inhibit the basophillic staining for your H&E. Otherwise it is actually just as easy as Rene states below. Good Luck, Glen Dawson -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Wednesday, August 13, 2008 10:27 AM To: histonet@lists.utsouthwestern.edu; Price, Tiffany Subject: Re: [Histonet] re-staining an IHC slide for H&E Just take the coverslip off, hydrate and stain as usual. Ren? J. --- On Wed, 8/13/08, Price, Tiffany wrote: From: Price, Tiffany Subject: [Histonet] re-staining an IHC slide for H&E To: histonet@lists.utsouthwestern.edu Date: Wednesday, August 13, 2008, 9:51 AM I have been asked to take a negative control slide and re-stain it for H&E. Does anyone have a protocol for this? Thanks Confidentiality Notice: This email message including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privilege information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy ALL copies of the message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From o.m.gallagher <@t> sheffield.ac.uk Wed Aug 13 11:01:28 2008 From: o.m.gallagher <@t> sheffield.ac.uk (Orla Gallagher) Date: Wed Aug 13 11:01:35 2008 Subject: [Histonet] MacNeal's tetrachrome Message-ID: <48A31368.31184.D49294@localhost> Dear Histonetters, Our lab would like to try staining some undecalcified LR White- embedded bone sections with MacNeal's tetrachrome stain. I've found a recipe on the Histonet archive which mentions making up the stain from scratch but doesn't say how long to stain for or how to treat the sections after staining. The recipe also mentions etching in formic acid before staining. Has anyone got some advice as I haven't tried any etching techniques before? Many thanks, Orla ****************************** Ms. Orla Gallagher Academic Unit of Bone Biology D Floor (DU20) Medical School Henry Wellcome Laboratories for Medical Research University of Sheffield Beech Hill Road Sheffield S10 2RX www.sheffield.ac.uk E-mail:o.m.gallagher@sheffield.ac.uk Tel: 0114 271 3783(lab) 0114 271 3337(office) Fax: 0114 271 1711 From ree3 <@t> leicester.ac.uk Wed Aug 13 11:09:13 2008 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Wed Aug 13 11:09:19 2008 Subject: [Histonet] Chicken secondarys Message-ID: <7722595275A4DD4FA225B92CDBF174A17438DEDC0E@EXC-MBX3.cfs.le.ac.uk> Best source of peroxidase labelled second antibodies to chicken primaries(IgGY), and this is not a yoking matter, all white!!. Many thanks Richard Edwards University of Leicester From kbowden <@t> ucsd.edu Wed Aug 13 11:21:04 2008 From: kbowden <@t> ucsd.edu (kbowden) Date: Wed Aug 13 11:21:51 2008 Subject: [Histonet] MacNeal's tetrachrome In-Reply-To: <48A31368.31184.D49294@localhost> References: <48A31368.31184.D49294@localhost> Message-ID: <48A309F0.7090003@ucsd.edu> 5% MC NEAL'S STAIN from the lab of Dr. Robert Schenk Bern Switzerland Milled/Surface Stain 1. Agitate in 50% EtOH 10 to 15 min. 2. Distilled water wash 3 min. 3. Agitate in 0.1% Formic acid 5 to 10 min. 4. Distilled water wash 3 min. 5. Distilled water wash 3 min. 6. Distilled water wash 3 min. 7. 5% Mc Neal's (Filtered) 5 min. 8. Distilled water wash 3 min. 9. Distilled water wash 3 min. 10. Distilled water wash 3 min. 11. 70% EtOH 1 dip 12. 0.1% Aqueous Basic Fuchsin (Filtered) 15 to 30 sec 13. Distilled water wash 3 min. 14. Distilled water wash 3 min. 15. Distilled water wash 3 min. 16. Air dry 5% Mc Neal's Stain Mod. Mc Neal's Tetra chrome (KEEP IN DARK) 5 ml. 0.1% Toluidine Blue aqueous 2.5 ml. Distilled Water 42.5 ml. Stir on Stir plate, filter afterwards 0.1% Basic Fuchsin Basic Fuchsin 0.5 gm Deionized water 500.0 ml 0.1% Formic Acid Formic Acid 1.1 ml Deionized water 1000.0 ml 10% Mod. Mc Neal's Tetrachrome (STOCK) Methylene Blue Chloride (Merck 6045-color 52015) 1.0 grams Azure A Eosinate (Chroma 1A 328) 1.6 grams Methyl Violet, Bernthsim (Chrome 1A 098) 0.2 gram Methyl Alcohol 500 ml Glycerin 500 ml Heat at 50?C for 1 day, then 37?C for 3 days in oven (capped). Filter and store in dark bottle in dark. Results: bone - reddish/purple cells/nuclei - blue Cartilage - blue/purple ligamenti, connective tissue - pinkish/red -- Karen Bowden Staff Research Associate II University of CA, San Diego Department of Orthopedics 9500 Gilman Dr. 0630 La Jolla, CA 92093-0630 858-534-4655 voice 858-534-5304 fax CONFIDENTIALITY NOTICE: THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND DELETE THE MATERIAL FROM ANY COMPUTER. Orla Gallagher wrote: > Dear Histonetters, > > Our lab would like to try staining some undecalcified LR White- > embedded bone sections with MacNeal's tetrachrome stain. I've > found a recipe on the Histonet archive which mentions making up > the stain from scratch but doesn't say how long to stain for or how > to treat the sections after staining. The recipe also mentions > etching in formic acid before staining. Has anyone got some advice > as I haven't tried any etching techniques before? > > Many thanks, > Orla > ****************************** > > Ms. Orla Gallagher > Academic Unit of Bone Biology > D Floor (DU20) Medical School > Henry Wellcome Laboratories for Medical Research > University of Sheffield > Beech Hill Road > Sheffield S10 2RX > www.sheffield.ac.uk > > E-mail:o.m.gallagher@sheffield.ac.uk > Tel: 0114 271 3783(lab) > 0114 271 3337(office) > Fax: 0114 271 1711 > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Margaret.Perry <@t> sdstate.edu Wed Aug 13 11:23:24 2008 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Wed Aug 13 11:23:31 2008 Subject: [Histonet] species control blocks Message-ID: I have a question for the people who do animal diagnostic work. How long do you keep paraffin species control blocks for such tests as chromogranin a, CD3, CD79a, S100a, cytokeratin etc. Have you noticed a decline in antigenicity after a period of time? How long is it before you notice a decline? Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 From gayle.callis <@t> bresnan.net Wed Aug 13 11:32:29 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Wed Aug 13 11:32:35 2008 Subject: [Histonet] MacNeal's tetrachrome References: <48A31368.31184.D49294@localhost> Message-ID: <000a01c8fd62$2def29d0$6601a8c0@Sunney> I will forward the method to you. Do NOT purchase this stain as a commercial preparation. It works best at an inhouse preparation. You will need to try the stain on the bone, as timing can vary with age of patient be it human or animal, also species, and bone type. HOever if you are doing LR white, just how thick are the sections? We did this stain on thick, slab sections as a surface stain. You may not need to etch the bone for a really thin section (?) Etching is done by immersing the bone into 1% formic acid (made from from stock 88%), for 30 sec to 1 min. Using a sonicator inproves etching, but the section must be rinsed well after etching with running tap water, then dried followed by staining. If the section is super thin, then timing can be reduced plus concentration of the formic acid to 0.5%. I will forward to you after this message. Gayle M. Callis HTL/HT/MT(ASCP) Bozeman MT ----- Original Message ----- From: "Orla Gallagher" To: Sent: Wednesday, August 13, 2008 10:01 AM Subject: [Histonet] MacNeal's tetrachrome > Dear Histonetters, > > Our lab would like to try staining some undecalcified LR White- > embedded bone sections with MacNeal's tetrachrome stain. I've > found a recipe on the Histonet archive which mentions making up > the stain from scratch but doesn't say how long to stain for or how > to treat the sections after staining. The recipe also mentions > etching in formic acid before staining. Has anyone got some advice > as I haven't tried any etching techniques before? > > Many thanks, > Orla > ****************************** > > Ms. Orla Gallagher > Academic Unit of Bone Biology > D Floor (DU20) Medical School > Henry Wellcome Laboratories for Medical Research > University of Sheffield > Beech Hill Road > Sheffield S10 2RX > www.sheffield.ac.uk > > E-mail:o.m.gallagher@sheffield.ac.uk > Tel: 0114 271 3783(lab) > 0114 271 3337(office) > Fax: 0114 271 1711 > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Aug 13 11:49:11 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Aug 13 11:49:15 2008 Subject: [Histonet] species control blocks In-Reply-To: Message-ID: <579807.3055.qm@web65704.mail.ac4.yahoo.com> During prolonged storage the epitope oxidizes and weaken. The weakening rate vary with the epitope, some are quite resistant and others weaken in 1 or 2 weeks. The rule of thumb is to cut enough controls that will last one week. It is better to cut both case and control of those weakening fast, that to lose all the reagents using one control already oxidized. Ren? J. --- On Wed, 8/13/08, Perry, Margaret wrote: From: Perry, Margaret Subject: [Histonet] species control blocks To: histonet@lists.utsouthwestern.edu Date: Wednesday, August 13, 2008, 12:23 PM I have a question for the people who do animal diagnostic work. How long do you keep paraffin species control blocks for such tests as chromogranin a, CD3, CD79a, S100a, cytokeratin etc. Have you noticed a decline in antigenicity after a period of time? How long is it before you notice a decline? Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lilbullrider00 <@t> yahoo.com Wed Aug 13 12:02:41 2008 From: lilbullrider00 <@t> yahoo.com (brent hart) Date: Wed Aug 13 12:02:46 2008 Subject: [Histonet] cork disection boards Message-ID: <559705.10930.qm@web53409.mail.re2.yahoo.com> what do you all out there use to pin small specimens such as mucosal resection for shipping to a lab, or to send to the grossing room. From Jacqueline.Farnsworth <@t> cls.ab.ca Wed Aug 13 12:04:32 2008 From: Jacqueline.Farnsworth <@t> cls.ab.ca (Jacqueline.Farnsworth@cls.ab.ca) Date: Wed Aug 13 12:04:46 2008 Subject: [Histonet] cork disection boards In-Reply-To: <559705.10930.qm@web53409.mail.re2.yahoo.com> Message-ID: <1E832A8226461B4B83A586FF052EBB371385C2@Mail3.calgary.com> We use small (homemade)wax boards. (We re-use old wax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of brent hart Sent: August 13, 2008 11:03 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] cork disection boards what do you all out there use to pin small specimens such as mucosal resection for shipping to a lab, or to send to the grossing room. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. From SwainFrancesL <@t> uams.edu Wed Aug 13 12:22:01 2008 From: SwainFrancesL <@t> uams.edu (Swain, Frances L) Date: Wed Aug 13 12:22:18 2008 Subject: [Histonet] eGFP Protein Message-ID: One of my PI's is wanting to identifiy eGFP in not only frozen sections but also paraffin sections. These will be mouse aortas is that possible? Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Jacqueline.Farnsworth <@t> cls.ab.ca Wed Aug 13 12:23:43 2008 From: Jacqueline.Farnsworth <@t> cls.ab.ca (Jacqueline.Farnsworth@cls.ab.ca) Date: Wed Aug 13 12:23:46 2008 Subject: [Histonet] slide labels? Message-ID: <1E832A8226461B4B83A586FF052EBB371385C5@Mail3.calgary.com> Hi all. We are currently exploring automatic slide labelling system (barcodes) for the first time! Does anyone have any label stock that they would recommend? Solvent proof/stain proof, etc.? We currently use Sakura tape coverslipper and the labels we've tried so far are slightly too long and interfere with the long size of the coverslips. Thank you in advance! Jacquie Farnsworth Anatomic Pathology Calgary Laboratory Services CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. From kevinclayton1979 <@t> gmail.com Wed Aug 13 13:10:36 2008 From: kevinclayton1979 <@t> gmail.com (Kevin Clayton) Date: Wed Aug 13 13:10:41 2008 Subject: [Histonet] Users of Prolong Gold Antifade from Invitrogen Message-ID: <94c6d26c0808131110j7bfbc72cm565cd3f02b8ea3a8@mail.gmail.com> Hey all Anyone out there know how dry your samples (sections/cells) should be before mounting using Prolong Gold? I have noticed (rarely) that sometimes all fluorescent signal - including DAPI - is much greater in some specimens than others from the same slide and otherwise treated the exact same. The product guidelines says to "remove any excess liquid from the specimen" but how much excess is excess? I generally try to remove liquid drops and letting visible liquid evaporate - without actually drying them - but a balance is difficult to achieve and it is impossible to get all samples from an experiment to the same level of "dryness". (And, man, do things dry quickly here in Spain compared to Ireland!) There's generally no problems with my samples but the concern is that the fluorescent signal/background could be affected, which would be a big problem when comparing signal strength within experiments. If someone knows that would be great (and it would save me the obvious and fairly simple test I should really have done ages ago!). Thanks, Kevin From gayle.callis <@t> bresnan.net Wed Aug 13 13:19:57 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Wed Aug 13 13:20:07 2008 Subject: [Histonet] eGFP Protein References: Message-ID: <000b01c8fd71$31815370$6601a8c0@Sunney> Frances, Yes, possible What are you planning to do with the tissues being frozen-- either prefixing and cyroprotecting or cutting fresh frozen tissues? How the tissue is handled initially will often determine seeing the eGFP. For frozen sections here, including undecalcified bone frozens, if we fix a fresh FS with a solvent, we detect our GFP via immunofluorescent staining. AntiGFP (either Goat or Rabbit - Rockland) and coming back with a secondary labelled with FITC or Alexa 488. We have found our solvent fixation ruins the GFP, a well known problem. Ig you are going to use IFA staining for FFPE tissues, Teri Johnson prefers rabbit antiGFP instead of goat antiGFP - she says is has given her less background staining with the FFPE. Since we do only frozen sections, we like the goat, antiGFP, but either with work. We buy our FITC conjugated secondaries from Jackson, usually made in Donkey, adsorbed to species being stained and also F(ab')2 frag of IgG whenever possible. With formalin fixed tissues, it may better to detect the eGFP with antibody, and come back with a secondary labelled with either FITC or Alexa 488. We always use a green fluorophore so it matches the color of eGFP. We have cut unfixed frozen sections, let them air dry and NOT FIX but mount the dry section with Molecular Probes prolong gold with DAPI although we have used it without DAPI too. These need to be looked asap, usually the same day for us. We do NOT like PFA or NBF fixed tissues for frozen sections due to the aldehyde induced autofluorescence as this can mess up seeing a weak GFP signel. There are ways to help reduce autofluorescence chemically. Go to IHCworld website, and click on fluroescence and autofluorescence. There is a link to a group in Toronto that is a superb discussion of autofluorescence and how to get rid of it. For FFPE tissue, we have use the glycine method to remove free aldhydes. It is simple and works well for our minimally fixed NBF tissue, no more than 8 hours, very thin pieces of tissue to guarantee good fixation. Good Luck Gayle M. Callis HTL/HT/MT(ASCP) ----- Original Message ----- From: "Swain, Frances L" To: Sent: Wednesday, August 13, 2008 11:22 AM Subject: [Histonet] eGFP Protein One of my PI's is wanting to identifiy eGFP in not only frozen sections but also paraffin sections. These will be mouse aortas is that possible? Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mickie25 <@t> netzero.net Wed Aug 13 13:22:02 2008 From: mickie25 <@t> netzero.net (Mickie Johnson) Date: Wed Aug 13 13:22:11 2008 Subject: [Histonet] cork disection boards In-Reply-To: <559705.10930.qm@web53409.mail.re2.yahoo.com> References: <559705.10930.qm@web53409.mail.re2.yahoo.com> Message-ID: Sheet Styrofoam is nice. You can pin out the specimen and then turn it over in a rubber maid container of formalin. We have done this for years with colons, stomachs, esophagus, etc. Mickie Mickie Johnson, B.S., HTL(ASCP) Mohs Histology Consulting Services, LLC & Mohs Lab Staffing 2507 S. Manito Blvd. Spokane, WA 99203 509-954-7134 FAX 509-624-3926 Web: www.mohshistogyconsulting.com & www.mohslabstaffing.com Email: mickie25@netzero.net DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of brent hart Sent: Wednesday, August 13, 2008 10:03 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] cork disection boards what do you all out there use to pin small specimens such as mucosal resection for shipping to a lab, or to send to the grossing room. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Internal Virus Database is out of date. Checked by AVG - http://www.avg.com Version: 8.0.134 / Virus Database: 270.4.5/1533 - Release Date: 7/3/2008 7:19 PM From b-frederick <@t> northwestern.edu Wed Aug 13 13:22:23 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Aug 13 13:22:30 2008 Subject: [Histonet] cork disection boards In-Reply-To: <559705.10930.qm@web53409.mail.re2.yahoo.com> Message-ID: <000001c8fd71$8818af30$d00f7ca5@lurie.northwestern.edu> I've seen people use Styrofoam as well as small cork boards. Jacqueline- we used to reuse old wax too- poured it into a cafeteria tray. For small "boards" we'd por it into the lis of the boxes slides ccome in. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of brent hart Sent: Wednesday, August 13, 2008 12:03 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] cork disection boards what do you all out there use to pin small specimens such as mucosal resection for shipping to a lab, or to send to the grossing room. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Wed Aug 13 13:23:42 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Aug 13 13:23:54 2008 Subject: [Histonet] cork disection boards In-Reply-To: <559705.10930.qm@web53409.mail.re2.yahoo.com> Message-ID: <000101c8fd71$ba2b70c0$d00f7ca5@lurie.northwestern.edu> That was "lid" not "lis" sorry. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of brent hart Sent: Wednesday, August 13, 2008 12:03 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] cork disection boards what do you all out there use to pin small specimens such as mucosal resection for shipping to a lab, or to send to the grossing room. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rcharles <@t> state.pa.us Wed Aug 13 13:53:40 2008 From: rcharles <@t> state.pa.us (Charles, Roger) Date: Wed Aug 13 13:53:47 2008 Subject: [Histonet] chlamydia psittacii control tissue Message-ID: <1B2F365C5F88A946B7D602E64ACD9CDD18F59A9B@ENHBGMBX04.PA.LCL> Hello all, I'm looking for control tissue of ovine, caprine, or avian origin for Chlamydia IHC testing. Willing to pay shipping and/or trade for other controls. thanks Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 From gayle.callis <@t> bresnan.net Wed Aug 13 13:55:57 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Wed Aug 13 13:56:03 2008 Subject: [Histonet] Users of Prolong Gold Antifade from Invitrogen References: <94c6d26c0808131110j7bfbc72cm565cd3f02b8ea3a8@mail.gmail.com> Message-ID: <002001c8fd76$38bae5c0$6601a8c0@Sunney> We never let out sections dry out before applying Prolong Gold antifade mounting media. Blot the excess liquid from around the section and apply the drop directly over the section. If too much PBS is removed, we have problems with bubble formation. We are very generous with the drops, even as expensive as this media is. We interpret removing excess liquid the same as blotting excess buffer from around a section during a staining protocol. I suggest you contact their tech services (Molecular Probes site of Invtirogen) and ask them this same question. They are prompt and very helpful. We have used Prolong Gold with DAPI on DSred label of fresh tissue (unfixed) frozen section, air dried for 30 minutes, then coverslipped. We did examine the section the same day and had success but this is with DSred, very similar in structure to GFP and NOT a fluorophore e.g. Alexa dyes, FITC, TRITC etc. etc. We had to do this since the DSred was compromised by loss for fluorescence due to either acetone or our favored acetone/alcohol fixation and autofluorescent issues with aldehyde fixatives. The latter is a problem with GFP too. Montana, USA has low humidity too, particularly in the winter, very dry. Gayle M. Callis HTL/HT/MT(ASCP) ----- Original Message ----- From: "Kevin Clayton" To: Sent: Wednesday, August 13, 2008 12:10 PM Subject: [Histonet] Users of Prolong Gold Antifade from Invitrogen > Hey all > > Anyone out there know how dry your samples (sections/cells) should be > before > mounting using Prolong Gold? I have noticed (rarely) that sometimes all > fluorescent signal - including DAPI - is much greater in some specimens > than > others from the same slide and otherwise treated the exact same. The > product > guidelines says to "remove any excess liquid from the specimen" but how > much > excess is excess? I generally try to remove liquid drops and letting > visible > liquid evaporate - without actually drying them - but a balance is > difficult > to achieve and it is impossible to get all samples from an experiment to > the > same level of "dryness". (And, man, do things dry quickly here in Spain > compared to Ireland!) There's generally no problems with my samples but > the > concern is that the fluorescent signal/background could be affected, which > would be a big problem when comparing signal strength within experiments. > > If someone knows that would be great (and it would save me the obvious and > fairly simple test I should really have done ages ago!). > > Thanks, > > Kevin > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sheila_adey <@t> hotmail.com Wed Aug 13 14:19:01 2008 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Wed Aug 13 14:19:05 2008 Subject: [Histonet] cork disection boards In-Reply-To: <000101c8fd71$ba2b70c0$d00f7ca5@lurie.northwestern.edu> References: <559705.10930.qm@web53409.mail.re2.yahoo.com> <000101c8fd71$ba2b70c0$d00f7ca5@lurie.northwestern.edu> Message-ID: Our dr.s pin them out on a piece of paraffin. We make them by pouring paraffin onto the lids of the containers (use the lid as a mold).Sheila Adey HT MLTPort Huron HospitalMichigan> From: b-frederick@northwestern.edu> To: lilbullrider00@yahoo.com; Histonet@lists.utsouthwestern.edu> Date: Wed, 13 Aug 2008 13:23:42 -0500> Subject: RE: [Histonet] cork disection boards> CC: > > That was "lid" not "lis" sorry.> > > Bernice Frederick HTL (ASCP)> Northwestern University> Pathology Core Facility> ECOGPCO-RL > 710 N Fairbanks Court> Olson 8-421> Chicago,IL 60611> 312-503-3723> > > -----Original Message-----> From: histonet-bounces@lists.utsouthwestern.edu> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of brent hart> Sent: Wednesday, August 13, 2008 12:03 PM> To: Histonet@lists.utsouthwestern.edu> Subject: [Histonet] cork disection boards> > what do you all out there use to pin small specimens such as mucosal> resection for shipping to a lab, or to send to the grossing room. > > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ From petepath <@t> yahoo.com Wed Aug 13 13:52:57 2008 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Wed Aug 13 14:19:42 2008 Subject: [Histonet] Hospital Pathology - Picking up Specimens Message-ID: <930696.46319.qm@web45111.mail.sp1.yahoo.com> We used to have the various departments ( OR, endoscopy, L & D, outpatient ORs) ?drop off specimens in our lab. Not infrequently specimens would get lost on their travels. After a lot of? campaigning I convinced?our administration?to give me the staff to create our own currier service. After assessing the various sites for best times to pick up at each location to maximize our return, we set up a schedual of pick ups which would ?best keep our PAs busy with a constant flow of work. We make sure our curriers scrutinize the requisitions and only take those that are complete. Those that need clinical info or other things are left at the site with instructions on what is incomplete. This system is a vast improvement over waiting for drop offs. The PAs are always busy and we rarely have anything lost or missing. For staffing,?our curriers double ?as accessioning clerks making them knowlegable about all aspects of the process. If you can pull this off I recommend it. Stephen Peters M.D. ? From Susan.Weber2 <@t> va.gov Wed Aug 13 14:31:16 2008 From: Susan.Weber2 <@t> va.gov (Weber, Susan (VHACLE)) Date: Wed Aug 13 14:31:26 2008 Subject: [Histonet] slide labels? In-Reply-To: <1E832A8226461B4B83A586FF052EBB371385C5@Mail3.calgary.com> References: <1E832A8226461B4B83A586FF052EBB371385C5@Mail3.calgary.com> Message-ID: <16C83872A53F4346AA9C3A18E3A3AAB903F76E1F@VHAV10MSGA1.v10.med.va.gov> We are using the Sakura coverslipper also and found that the labels for the Ventana equipment also interfere with the tape. Solution: change the setting to 50mm instead of 55mm which we routinely coverslip at. We are using the Leica slide labeler (which also has the ability to print barcodes as well) this prevents us from having to affix a separate label to the slide, but Time-Med has a Xylene/Alcohol proof label which I have previously used with a Sato Printer. Susan M Weber HT(ASCP) Histology Supervisor Louis Stokes Cleveland VA Medical Center 10701 East Blvd Cleveland, Ohio 44106 (216) 791-3800 X6154 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jacqueline.Farnsworth@cls.ab.ca Sent: Wednesday, August 13, 2008 1:24 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] slide labels? Hi all. We are currently exploring automatic slide labelling system (barcodes) for the first time! Does anyone have any label stock that they would recommend? Solvent proof/stain proof, etc.? We currently use Sakura tape coverslipper and the labels we've tried so far are slightly too long and interfere with the long size of the coverslips. Thank you in advance! Jacquie Farnsworth Anatomic Pathology Calgary Laboratory Services CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Wed Aug 13 14:32:43 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed Aug 13 14:32:48 2008 Subject: [Histonet] PSLIM Message-ID: <57BE698966D5C54EAE8612E8941D7683038CCD32@EXCHANGE3.huntingtonhospital.com> I know this product has been discussed recently, but could I get some feedback on the PSLIM slide writer? (I could not get into the archives) Laurie Colbert From petepath <@t> yahoo.com Wed Aug 13 13:52:57 2008 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Wed Aug 13 14:34:09 2008 Subject: [Histonet] Hospital Pathology - Picking up Specimens Message-ID: <930696.46319.qm@web45111.mail.sp1.yahoo.com> We used to have the various departments ( OR, endoscopy, L & D, outpatient ORs) ?drop off specimens in our lab. Not infrequently specimens would get lost on their travels. After a lot of? campaigning I convinced?our administration?to give me the staff to create our own currier service. After assessing the various sites for best times to pick up at each location to maximize our return, we set up a schedual of pick ups which would ?best keep our PAs busy with a constant flow of work. We make sure our curriers scrutinize the requisitions and only take those that are complete. Those that need clinical info or other things are left at the site with instructions on what is incomplete. This system is a vast improvement over waiting for drop offs. The PAs are always busy and we rarely have anything lost or missing. For staffing,?our curriers double ?as accessioning clerks making them knowlegable about all aspects of the process. If you can pull this off I recommend it. Stephen Peters M.D. ? From mhamilton <@t> GENVEC.com Wed Aug 13 14:49:29 2008 From: mhamilton <@t> GENVEC.com (Hamilton, Melissa) Date: Wed Aug 13 14:49:57 2008 Subject: [Histonet] Optimal Fixative Message-ID: <71CB4E18A03CB4448F2DD236C6C7C316017F0870@genexch.genvec.com> I am searching for a protocol for fixing mouse tumors (subcutaneously or orthotopically grown) that are approx 2 to 2.5 cm in diameter. I am having issues with penetration throughout the tissue. Thanks. From algranth <@t> u.arizona.edu Wed Aug 13 15:04:24 2008 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Wed Aug 13 15:04:24 2008 Subject: [Histonet] PSLIM In-Reply-To: <57BE698966D5C54EAE8612E8941D7683038CCD32@EXCHANGE3.hunting tonhospital.com> References: <57BE698966D5C54EAE8612E8941D7683038CCD32@EXCHANGE3.huntingtonhospital.com> Message-ID: <6.2.3.4.1.20080813130305.02701d60@algranth.inbox.email.arizona.edu> There were some inquiries about this product but it seems that nobody has used one. I am also interested in this printer and would like to have some feedback as well. Andi Grantham At 12:32 PM 8/13/2008, Laurie Colbert wrote: >I know this product has been discussed recently, but could I get some >feedback on the PSLIM slide writer? (I could not get into the archives) > > > >Laurie Colbert > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From liz <@t> premierlab.com Wed Aug 13 15:10:15 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Aug 13 15:10:42 2008 Subject: [Histonet] PSLIM In-Reply-To: <6.2.3.4.1.20080813130305.02701d60@algranth.inbox.email.arizona.edu> References: <57BE698966D5C54EAE8612E8941D7683038CCD32@EXCHANGE3.huntingtonhospital.com> <6.2.3.4.1.20080813130305.02701d60@algranth.inbox.email.arizona.edu> Message-ID: We have considering purchasing this instrument. We sent out some of our blank slides to several vendors to be printed on. In my opinion the PSLIM slides looked great. The printing was very clear. We still need to run the slides through some stains just to see how the label holds up after staining. But overall I was impressed by the quality. We might purchase one since the price point is low compared to what is out there. That's all I can comment on, since we have not personally used the instrument. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Wednesday, August 13, 2008 2:04 PM To: Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] PSLIM There were some inquiries about this product but it seems that nobody has used one. I am also interested in this printer and would like to have some feedback as well. Andi Grantham At 12:32 PM 8/13/2008, Laurie Colbert wrote: >I know this product has been discussed recently, but could I get some >feedback on the PSLIM slide writer? (I could not get into the >archives) > > > >Laurie Colbert > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Wed Aug 13 15:20:29 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Wed Aug 13 15:20:38 2008 Subject: [Histonet] PSLIM In-Reply-To: Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A38D2@IS-E2K3.grhs.net> Remember to also look at the ink print cartridge replacement cost. This varies GREATLY with slide printers. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, August 13, 2008 3:10 PM To: Andrea Grantham; Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM We have considering purchasing this instrument. We sent out some of our blank slides to several vendors to be printed on. In my opinion the PSLIM slides looked great. The printing was very clear. We still need to run the slides through some stains just to see how the label holds up after staining. But overall I was impressed by the quality. We might purchase one since the price point is low compared to what is out there. That's all I can comment on, since we have not personally used the instrument. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Wednesday, August 13, 2008 2:04 PM To: Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] PSLIM There were some inquiries about this product but it seems that nobody has used one. I am also interested in this printer and would like to have some feedback as well. Andi Grantham At 12:32 PM 8/13/2008, Laurie Colbert wrote: >I know this product has been discussed recently, but could I get some >feedback on the PSLIM slide writer? (I could not get into the >archives) > > > >Laurie Colbert > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tim.morken <@t> thermofisher.com Wed Aug 13 15:25:27 2008 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Wed Aug 13 15:25:57 2008 Subject: [Histonet] PSLIM In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A38D2@IS-E2K3.grhs.net> References: <661949901A768E4F9CC16D8AF8F2838C017A38D2@IS-E2K3.grhs.net> Message-ID: <6BFF6D137DF6BC43B33891BA96E83B1901BFCC9A@PGHCR-EXMB-VS-1.na.fshrnet.com> They say 5K price for labeler, $49.00 ribbon that labels 12,000 slides. www.accuplace.com Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, August 13, 2008 1:20 PM To: Liz Chlipala; Andrea Grantham; Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM Remember to also look at the ink print cartridge replacement cost. This varies GREATLY with slide printers. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, August 13, 2008 3:10 PM To: Andrea Grantham; Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM We have considering purchasing this instrument. We sent out some of our blank slides to several vendors to be printed on. In my opinion the PSLIM slides looked great. The printing was very clear. We still need to run the slides through some stains just to see how the label holds up after staining. But overall I was impressed by the quality. We might purchase one since the price point is low compared to what is out there. That's all I can comment on, since we have not personally used the instrument. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Wednesday, August 13, 2008 2:04 PM To: Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] PSLIM There were some inquiries about this product but it seems that nobody has used one. I am also interested in this printer and would like to have some feedback as well. Andi Grantham At 12:32 PM 8/13/2008, Laurie Colbert wrote: >I know this product has been discussed recently, but could I get some >feedback on the PSLIM slide writer? (I could not get into the >archives) > > > >Laurie Colbert > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Wed Aug 13 15:46:39 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Aug 13 15:46:45 2008 Subject: [Histonet] PSLIM In-Reply-To: <6BFF6D137DF6BC43B33891BA96E83B1901BFCC9A@PGHCR-EXMB-VS-1.na.fshrnet.com> References: <661949901A768E4F9CC16D8AF8F2838C017A38D2@IS-E2K3.grhs.net> <6BFF6D137DF6BC43B33891BA96E83B1901BFCC9A@PGHCR-EXMB-VS-1.na.fshrnet.com> Message-ID: 5 K for the instrument - then if you want to use the codesoft software $500.00. The kicker is the training which costs - 1 day west of the missippippi is $2000.00 two days west of the missippippi is $3000.00. Then if you purchase the 3 year support package your talking about up to 10 K total once its all said and done. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Wednesday, August 13, 2008 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM They say 5K price for labeler, $49.00 ribbon that labels 12,000 slides. www.accuplace.com Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, August 13, 2008 1:20 PM To: Liz Chlipala; Andrea Grantham; Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM Remember to also look at the ink print cartridge replacement cost. This varies GREATLY with slide printers. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, August 13, 2008 3:10 PM To: Andrea Grantham; Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM We have considering purchasing this instrument. We sent out some of our blank slides to several vendors to be printed on. In my opinion the PSLIM slides looked great. The printing was very clear. We still need to run the slides through some stains just to see how the label holds up after staining. But overall I was impressed by the quality. We might purchase one since the price point is low compared to what is out there. That's all I can comment on, since we have not personally used the instrument. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Wednesday, August 13, 2008 2:04 PM To: Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] PSLIM There were some inquiries about this product but it seems that nobody has used one. I am also interested in this printer and would like to have some feedback as well. Andi Grantham At 12:32 PM 8/13/2008, Laurie Colbert wrote: >I know this product has been discussed recently, but could I get some >feedback on the PSLIM slide writer? (I could not get into the >archives) > > > >Laurie Colbert > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> pathview.com Wed Aug 13 16:13:45 2008 From: mike <@t> pathview.com (Michael Mihalik) Date: Wed Aug 13 16:14:57 2008 Subject: [Histonet] PSLIM In-Reply-To: References: <661949901A768E4F9CC16D8AF8F2838C017A38D2@IS-E2K3.grhs.net> <6BFF6D137DF6BC43B33891BA96E83B1901BFCC9A@PGHCR-EXMB-VS-1.na.fshrnet.com> Message-ID: <00d601c8fd89$86e06960$94a13c20$@com> It really depends on what you're after. To have the slide preprinted with information and NO labels is pretty cool. And the pslim printer and gear can help make up for a deficient information system. (you can have the printer have the smarts to print case #, etc. on the slide) On the other hand, a $500.00 zebra printer with xylene resistant labels works fine in our application. We use 2D barcodes and the histotech places the slide on the label just prior to cutting the block. They do this one slide at a time as they scan the barcode on the cassette. So, some things to think about: 1. printing dynamic at the cutting station or in batch. If in batch, you could maybe get away with one or two printers. If you want these at the cutting stations, then you might need more printers. 2. you have to have certain slides for the pslim, but you have to have labels for the zebra solution AND then you also have to have your information system vendor or someone else write the software to tell the zebra what to print. All that being said, I'm intrigued by the idea of this product, but at my current installation, I'd be talking about 7 printers. That's a lot of up front cost. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, August 13, 2008 4:47 PM To: Morken, Tim; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM 5 K for the instrument - then if you want to use the codesoft software $500.00. The kicker is the training which costs - 1 day west of the missippippi is $2000.00 two days west of the missippippi is $3000.00. Then if you purchase the 3 year support package your talking about up to 10 K total once its all said and done. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Wednesday, August 13, 2008 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM They say 5K price for labeler, $49.00 ribbon that labels 12,000 slides. www.accuplace.com Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, August 13, 2008 1:20 PM To: Liz Chlipala; Andrea Grantham; Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM Remember to also look at the ink print cartridge replacement cost. This varies GREATLY with slide printers. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, August 13, 2008 3:10 PM To: Andrea Grantham; Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM We have considering purchasing this instrument. We sent out some of our blank slides to several vendors to be printed on. In my opinion the PSLIM slides looked great. The printing was very clear. We still need to run the slides through some stains just to see how the label holds up after staining. But overall I was impressed by the quality. We might purchase one since the price point is low compared to what is out there. That's all I can comment on, since we have not personally used the instrument. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Wednesday, August 13, 2008 2:04 PM To: Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] PSLIM There were some inquiries about this product but it seems that nobody has used one. I am also interested in this printer and would like to have some feedback as well. Andi Grantham At 12:32 PM 8/13/2008, Laurie Colbert wrote: >I know this product has been discussed recently, but could I get some >feedback on the PSLIM slide writer? (I could not get into the >archives) > > > >Laurie Colbert > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From annette_hall <@t> pa-ucl.com Wed Aug 13 16:49:19 2008 From: annette_hall <@t> pa-ucl.com (Annette Hall) Date: Wed Aug 13 16:49:26 2008 Subject: [Histonet] PSLIM In-Reply-To: <00d601c8fd89$86e06960$94a13c20$@com> Message-ID: <71320B4EBC7C15419563EAFBFCD924651EDA553E6F@hades.pa-ucl.com> Has anyone attempted to interface this slide printer with Cerner's CoPath system? Annette J Hall United Clinical Labs Dubuque, IA 52001 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Wednesday, August 13, 2008 4:14 PM To: 'Liz Chlipala'; 'Morken, Tim'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM It really depends on what you're after. To have the slide preprinted with information and NO labels is pretty cool. And the pslim printer and gear can help make up for a deficient information system. (you can have the printer have the smarts to print case #, etc. on the slide) On the other hand, a $500.00 zebra printer with xylene resistant labels works fine in our application. We use 2D barcodes and the histotech places the slide on the label just prior to cutting the block. They do this one slide at a time as they scan the barcode on the cassette. So, some things to think about: 1. printing dynamic at the cutting station or in batch. If in batch, you could maybe get away with one or two printers. If you want these at the cutting stations, then you might need more printers. 2. you have to have certain slides for the pslim, but you have to have labels for the zebra solution AND then you also have to have your information system vendor or someone else write the software to tell the zebra what to print. All that being said, I'm intrigued by the idea of this product, but at my current installation, I'd be talking about 7 printers. That's a lot of up front cost. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, August 13, 2008 4:47 PM To: Morken, Tim; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM 5 K for the instrument - then if you want to use the codesoft software $500.00. The kicker is the training which costs - 1 day west of the missippippi is $2000.00 two days west of the missippippi is $3000.00. Then if you purchase the 3 year support package your talking about up to 10 K total once its all said and done. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Wednesday, August 13, 2008 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM They say 5K price for labeler, $49.00 ribbon that labels 12,000 slides. www.accuplace.com Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, August 13, 2008 1:20 PM To: Liz Chlipala; Andrea Grantham; Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM Remember to also look at the ink print cartridge replacement cost. This varies GREATLY with slide printers. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, August 13, 2008 3:10 PM To: Andrea Grantham; Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM We have considering purchasing this instrument. We sent out some of our blank slides to several vendors to be printed on. In my opinion the PSLIM slides looked great. The printing was very clear. We still need to run the slides through some stains just to see how the label holds up after staining. But overall I was impressed by the quality. We might purchase one since the price point is low compared to what is out there. That's all I can comment on, since we have not personally used the instrument. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Wednesday, August 13, 2008 2:04 PM To: Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] PSLIM There were some inquiries about this product but it seems that nobody has used one. I am also interested in this printer and would like to have some feedback as well. Andi Grantham At 12:32 PM 8/13/2008, Laurie Colbert wrote: >I know this product has been discussed recently, but could I get some >feedback on the PSLIM slide writer? (I could not get into the >archives) > > > >Laurie Colbert > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From mike <@t> pathview.com Wed Aug 13 18:02:22 2008 From: mike <@t> pathview.com (Michael Mihalik) Date: Wed Aug 13 18:03:59 2008 Subject: [Histonet] PSLIM In-Reply-To: <71320B4EBC7C15419563EAFBFCD924651EDA553E6F@hades.pa-ucl.com> References: <00d601c8fd89$86e06960$94a13c20$@com> <71320B4EBC7C15419563EAFBFCD924651EDA553E6F@hades.pa-ucl.com> Message-ID: <00ec01c8fd98$c0d0e0f0$4272a2d0$@com> Btw, along those lines, how many of you are thinking of using bar codes with this or any other slide printer? I'm just trying to determine whether just putting the case # and other information on the slide is so valuable, or have we finally come to the point where everyone naturally thinks of putting barcodes on their slides. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: Annette Hall [mailto:annette_hall@pa-ucl.com] Sent: Wednesday, August 13, 2008 5:49 PM To: 'Michael Mihalik'; 'Liz Chlipala'; 'Morken, Tim'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM Has anyone attempted to interface this slide printer with Cerner's CoPath system? Annette J Hall United Clinical Labs Dubuque, IA 52001 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Wednesday, August 13, 2008 4:14 PM To: 'Liz Chlipala'; 'Morken, Tim'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM It really depends on what you're after. To have the slide preprinted with information and NO labels is pretty cool. And the pslim printer and gear can help make up for a deficient information system. (you can have the printer have the smarts to print case #, etc. on the slide) On the other hand, a $500.00 zebra printer with xylene resistant labels works fine in our application. We use 2D barcodes and the histotech places the slide on the label just prior to cutting the block. They do this one slide at a time as they scan the barcode on the cassette. So, some things to think about: 1. printing dynamic at the cutting station or in batch. If in batch, you could maybe get away with one or two printers. If you want these at the cutting stations, then you might need more printers. 2. you have to have certain slides for the pslim, but you have to have labels for the zebra solution AND then you also have to have your information system vendor or someone else write the software to tell the zebra what to print. All that being said, I'm intrigued by the idea of this product, but at my current installation, I'd be talking about 7 printers. That's a lot of up front cost. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, August 13, 2008 4:47 PM To: Morken, Tim; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM 5 K for the instrument - then if you want to use the codesoft software $500.00. The kicker is the training which costs - 1 day west of the missippippi is $2000.00 two days west of the missippippi is $3000.00. Then if you purchase the 3 year support package your talking about up to 10 K total once its all said and done. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Wednesday, August 13, 2008 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM They say 5K price for labeler, $49.00 ribbon that labels 12,000 slides. www.accuplace.com Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, August 13, 2008 1:20 PM To: Liz Chlipala; Andrea Grantham; Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM Remember to also look at the ink print cartridge replacement cost. This varies GREATLY with slide printers. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, August 13, 2008 3:10 PM To: Andrea Grantham; Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM We have considering purchasing this instrument. We sent out some of our blank slides to several vendors to be printed on. In my opinion the PSLIM slides looked great. The printing was very clear. We still need to run the slides through some stains just to see how the label holds up after staining. But overall I was impressed by the quality. We might purchase one since the price point is low compared to what is out there. That's all I can comment on, since we have not personally used the instrument. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Wednesday, August 13, 2008 2:04 PM To: Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] PSLIM There were some inquiries about this product but it seems that nobody has used one. I am also interested in this printer and would like to have some feedback as well. Andi Grantham At 12:32 PM 8/13/2008, Laurie Colbert wrote: >I know this product has been discussed recently, but could I get some >feedback on the PSLIM slide writer? (I could not get into the >archives) > > > >Laurie Colbert > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From annette_hall <@t> pa-ucl.com Wed Aug 13 18:09:57 2008 From: annette_hall <@t> pa-ucl.com (Annette Hall) Date: Wed Aug 13 18:10:02 2008 Subject: [Histonet] PSLIM In-Reply-To: <00ec01c8fd98$c0d0e0f0$4272a2d0$@com> Message-ID: <71320B4EBC7C15419563EAFBFCD924651EDA553E71@hades.pa-ucl.com> We currently use barcodes on our slide labels and would continue to do so. The pathologists (and cytotechs) scan the barcode and CoPath takes them to the correct case in the computer. This has been a valuable tool in reducing errors. I would not go back to strictly human identified slides. -----Original Message----- From: Michael Mihalik [mailto:mike@pathview.com] Sent: Wednesday, August 13, 2008 6:02 PM To: Annette Hall; 'Liz Chlipala'; 'Morken, Tim'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM Btw, along those lines, how many of you are thinking of using bar codes with this or any other slide printer? I'm just trying to determine whether just putting the case # and other information on the slide is so valuable, or have we finally come to the point where everyone naturally thinks of putting barcodes on their slides. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: Annette Hall [mailto:annette_hall@pa-ucl.com] Sent: Wednesday, August 13, 2008 5:49 PM To: 'Michael Mihalik'; 'Liz Chlipala'; 'Morken, Tim'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM Has anyone attempted to interface this slide printer with Cerner's CoPath system? Annette J Hall United Clinical Labs Dubuque, IA 52001 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Wednesday, August 13, 2008 4:14 PM To: 'Liz Chlipala'; 'Morken, Tim'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM It really depends on what you're after. To have the slide preprinted with information and NO labels is pretty cool. And the pslim printer and gear can help make up for a deficient information system. (you can have the printer have the smarts to print case #, etc. on the slide) On the other hand, a $500.00 zebra printer with xylene resistant labels works fine in our application. We use 2D barcodes and the histotech places the slide on the label just prior to cutting the block. They do this one slide at a time as they scan the barcode on the cassette. So, some things to think about: 1. printing dynamic at the cutting station or in batch. If in batch, you could maybe get away with one or two printers. If you want these at the cutting stations, then you might need more printers. 2. you have to have certain slides for the pslim, but you have to have labels for the zebra solution AND then you also have to have your information system vendor or someone else write the software to tell the zebra what to print. All that being said, I'm intrigued by the idea of this product, but at my current installation, I'd be talking about 7 printers. That's a lot of up front cost. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, August 13, 2008 4:47 PM To: Morken, Tim; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM 5 K for the instrument - then if you want to use the codesoft software $500.00. The kicker is the training which costs - 1 day west of the missippippi is $2000.00 two days west of the missippippi is $3000.00. Then if you purchase the 3 year support package your talking about up to 10 K total once its all said and done. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Wednesday, August 13, 2008 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM They say 5K price for labeler, $49.00 ribbon that labels 12,000 slides. www.accuplace.com Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, August 13, 2008 1:20 PM To: Liz Chlipala; Andrea Grantham; Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM Remember to also look at the ink print cartridge replacement cost. This varies GREATLY with slide printers. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, August 13, 2008 3:10 PM To: Andrea Grantham; Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM We have considering purchasing this instrument. We sent out some of our blank slides to several vendors to be printed on. In my opinion the PSLIM slides looked great. The printing was very clear. We still need to run the slides through some stains just to see how the label holds up after staining. But overall I was impressed by the quality. We might purchase one since the price point is low compared to what is out there. That's all I can comment on, since we have not personally used the instrument. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Wednesday, August 13, 2008 2:04 PM To: Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] PSLIM There were some inquiries about this product but it seems that nobody has used one. I am also interested in this printer and would like to have some feedback as well. Andi Grantham At 12:32 PM 8/13/2008, Laurie Colbert wrote: >I know this product has been discussed recently, but could I get some >feedback on the PSLIM slide writer? (I could not get into the >archives) > > > >Laurie Colbert > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From mike <@t> pathview.com Wed Aug 13 18:33:54 2008 From: mike <@t> pathview.com (Michael Mihalik) Date: Wed Aug 13 18:35:08 2008 Subject: [Histonet] PSLIM In-Reply-To: <71320B4EBC7C15419563EAFBFCD924651EDA553E71@hades.pa-ucl.com> References: <00ec01c8fd98$c0d0e0f0$4272a2d0$@com> <71320B4EBC7C15419563EAFBFCD924651EDA553E71@hades.pa-ucl.com> Message-ID: <00f701c8fd9d$1d3b56a0$57b203e0$@com> So basically, they're at a case # prompt and they scan the barcode, correct? It then pulls up a result entry screen...? Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: Annette Hall [mailto:annette_hall@pa-ucl.com] Sent: Wednesday, August 13, 2008 7:10 PM To: 'Michael Mihalik'; 'Liz Chlipala'; 'Morken, Tim'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM We currently use barcodes on our slide labels and would continue to do so. The pathologists (and cytotechs) scan the barcode and CoPath takes them to the correct case in the computer. This has been a valuable tool in reducing errors. I would not go back to strictly human identified slides. -----Original Message----- From: Michael Mihalik [mailto:mike@pathview.com] Sent: Wednesday, August 13, 2008 6:02 PM To: Annette Hall; 'Liz Chlipala'; 'Morken, Tim'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM Btw, along those lines, how many of you are thinking of using bar codes with this or any other slide printer? I'm just trying to determine whether just putting the case # and other information on the slide is so valuable, or have we finally come to the point where everyone naturally thinks of putting barcodes on their slides. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: Annette Hall [mailto:annette_hall@pa-ucl.com] Sent: Wednesday, August 13, 2008 5:49 PM To: 'Michael Mihalik'; 'Liz Chlipala'; 'Morken, Tim'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM Has anyone attempted to interface this slide printer with Cerner's CoPath system? Annette J Hall United Clinical Labs Dubuque, IA 52001 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Mihalik Sent: Wednesday, August 13, 2008 4:14 PM To: 'Liz Chlipala'; 'Morken, Tim'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM It really depends on what you're after. To have the slide preprinted with information and NO labels is pretty cool. And the pslim printer and gear can help make up for a deficient information system. (you can have the printer have the smarts to print case #, etc. on the slide) On the other hand, a $500.00 zebra printer with xylene resistant labels works fine in our application. We use 2D barcodes and the histotech places the slide on the label just prior to cutting the block. They do this one slide at a time as they scan the barcode on the cassette. So, some things to think about: 1. printing dynamic at the cutting station or in batch. If in batch, you could maybe get away with one or two printers. If you want these at the cutting stations, then you might need more printers. 2. you have to have certain slides for the pslim, but you have to have labels for the zebra solution AND then you also have to have your information system vendor or someone else write the software to tell the zebra what to print. All that being said, I'm intrigued by the idea of this product, but at my current installation, I'd be talking about 7 printers. That's a lot of up front cost. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, August 13, 2008 4:47 PM To: Morken, Tim; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM 5 K for the instrument - then if you want to use the codesoft software $500.00. The kicker is the training which costs - 1 day west of the missippippi is $2000.00 two days west of the missippippi is $3000.00. Then if you purchase the 3 year support package your talking about up to 10 K total once its all said and done. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Wednesday, August 13, 2008 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM They say 5K price for labeler, $49.00 ribbon that labels 12,000 slides. www.accuplace.com Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, August 13, 2008 1:20 PM To: Liz Chlipala; Andrea Grantham; Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM Remember to also look at the ink print cartridge replacement cost. This varies GREATLY with slide printers. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, August 13, 2008 3:10 PM To: Andrea Grantham; Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM We have considering purchasing this instrument. We sent out some of our blank slides to several vendors to be printed on. In my opinion the PSLIM slides looked great. The printing was very clear. We still need to run the slides through some stains just to see how the label holds up after staining. But overall I was impressed by the quality. We might purchase one since the price point is low compared to what is out there. That's all I can comment on, since we have not personally used the instrument. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Wednesday, August 13, 2008 2:04 PM To: Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] PSLIM There were some inquiries about this product but it seems that nobody has used one. I am also interested in this printer and would like to have some feedback as well. Andi Grantham At 12:32 PM 8/13/2008, Laurie Colbert wrote: >I know this product has been discussed recently, but could I get some >feedback on the PSLIM slide writer? (I could not get into the >archives) > > > >Laurie Colbert > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From shive003 <@t> umn.edu Wed Aug 13 18:56:29 2008 From: shive003 <@t> umn.edu (Jan Shivers) Date: Wed Aug 13 18:56:36 2008 Subject: [Histonet] eGFP Protein References: Message-ID: I have done it on paraffin sections with good results. But I am on vacation until the 25th; I'll give you more specifics when I return to the office. Jan Shivers Univ. of Minnesota Veterinary Diagnostic Lab ----- Original Message ----- From: "Swain, Frances L" To: Sent: Wednesday, August 13, 2008 12:22 PM Subject: [Histonet] eGFP Protein One of my PI's is wanting to identifiy eGFP in not only frozen sections but also paraffin sections. These will be mouse aortas is that possible? Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfrancesl@uams.edu email Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Wed Aug 13 20:08:04 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Aug 13 20:08:10 2008 Subject: [Histonet] PSLIM Message-ID: <582736990808131808m4d254a54jcf3ba537999e5efa@mail.gmail.com> Michael, I think that might be incorrect. I distinctly remember at the show that I saw the PSLIM printer at there was a variety of slides. I specifically looked for this as I HATE colormark slides and would give my left ... arm to not have to use the bloody things! So I would not want to get a printer locking me into a particular slide. Can anyone confirm this? Does anyone ACTUALLY have one? Is this a Jedi mind trick on us all? Amos Message: 23 Date: Wed, 13 Aug 2008 17:13:45 -0400 From: "Michael Mihalik" Subject: RE: [Histonet] PSLIM To: "'Liz Chlipala'" , "'Morken, Tim'" , Message-ID: <00d601c8fd89$86e06960$94a13c20$@com> Content-Type: text/plain; charset="us-ascii" << SNIP >> So, some things to think about: 1. printing dynamic at the cutting station or in batch. If in batch, you could maybe get away with one or two printers. If you want these at the cutting stations, then you might need more printers. 2. you have to have certain slides for the pslim, but you have to have labels for the zebra solution AND then you also have to have your information system vendor or someone else write the software to tell the zebra what to print. All that being said, I'm intrigued by the idea of this product, but at my current installation, I'd be talking about 7 printers. That's a lot of up front cost. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 From mike <@t> pathview.com Thu Aug 14 04:02:35 2008 From: mike <@t> pathview.com (Michael Mihalik) Date: Thu Aug 14 04:03:58 2008 Subject: [Histonet] PSLIM In-Reply-To: <582736990808131808m4d254a54jcf3ba537999e5efa@mail.gmail.com> References: <582736990808131808m4d254a54jcf3ba537999e5efa@mail.gmail.com> Message-ID: <000101c8fdec$8ffdc020$aff94060$@com> I'm sorry, perhaps I misspoke. This is a line from a sales rep.: Regarding your question about slides: PSLIM will print on frosted or colored slides. But as you mention, I would love to hear from someone who has this unit. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 From: Amos Brooks [mailto:amosbrooks@gmail.com] Sent: Wednesday, August 13, 2008 9:08 PM To: mike@pathview.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM Michael, I think that might be incorrect. I distinctly remember at the show that I saw the PSLIM printer at there was a variety of slides. I specifically looked for this as I HATE colormark slides and would give my left ... arm to not have to use the bloody things! So I would not want to get a printer locking me into a particular slide. Can anyone confirm this? Does anyone ACTUALLY have one? Is this a Jedi mind trick on us all? Amos Message: 23 Date: Wed, 13 Aug 2008 17:13:45 -0400 From: "Michael Mihalik" Subject: RE: [Histonet] PSLIM To: "'Liz Chlipala'" , "'Morken, Tim'" , Message-ID: <00d601c8fd89$86e06960$ 94a13c20$@com> Content-Type: text/plain; charset="us-ascii" << SNIP >> So, some things to think about: 1. printing dynamic at the cutting station or in batch. If in batch, you could maybe get away with one or two printers. If you want these at the cutting stations, then you might need more printers. 2. you have to have certain slides for the pslim, but you have to have labels for the zebra solution AND then you also have to have your information system vendor or someone else write the software to tell the zebra what to print. All that being said, I'm intrigued by the idea of this product, but at my current installation, I'd be talking about 7 printers. That's a lot of up front cost. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968 From JCollins <@t> palmbeachpath.com Thu Aug 14 06:06:10 2008 From: JCollins <@t> palmbeachpath.com (Judy Collins) Date: Thu Aug 14 06:06:16 2008 Subject: [Histonet] PSLIM In-Reply-To: References: <661949901A768E4F9CC16D8AF8F2838C017A38D2@IS-E2K3.grhs.net> <6BFF6D137DF6BC43B33891BA96E83B1901BFCC9A@PGHCR-EXMB-VS-1.na.fshrnet.com> Message-ID: <05CAE76AB5D5ED409864C6DD86F13349022B6197F9@pbpsflexch02.pbp.local> And don't forget you need one unit for each cutting station. Judy Collins -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, August 13, 2008 4:47 PM To: Morken, Tim; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM 5 K for the instrument - then if you want to use the codesoft software $500.00. The kicker is the training which costs - 1 day west of the missippippi is $2000.00 two days west of the missippippi is $3000.00. Then if you purchase the 3 year support package your talking about up to 10 K total once its all said and done. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim Sent: Wednesday, August 13, 2008 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM They say 5K price for labeler, $49.00 ribbon that labels 12,000 slides. www.accuplace.com Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Wednesday, August 13, 2008 1:20 PM To: Liz Chlipala; Andrea Grantham; Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM Remember to also look at the ink print cartridge replacement cost. This varies GREATLY with slide printers. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Wednesday, August 13, 2008 3:10 PM To: Andrea Grantham; Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] PSLIM We have considering purchasing this instrument. We sent out some of our blank slides to several vendors to be printed on. In my opinion the PSLIM slides looked great. The printing was very clear. We still need to run the slides through some stains just to see how the label holds up after staining. But overall I was impressed by the quality. We might purchase one since the price point is low compared to what is out there. That's all I can comment on, since we have not personally used the instrument. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Wednesday, August 13, 2008 2:04 PM To: Laurie Colbert; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] PSLIM There were some inquiries about this product but it seems that nobody has used one. I am also interested in this printer and would like to have some feedback as well. Andi Grantham At 12:32 PM 8/13/2008, Laurie Colbert wrote: >I know this product has been discussed recently, but could I get some >feedback on the PSLIM slide writer? (I could not get into the >archives) > > > >Laurie Colbert > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Thu Aug 14 08:55:15 2008 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Aug 14 08:54:35 2008 Subject: [Histonet] Optimal Fixative In-Reply-To: <71CB4E18A03CB4448F2DD236C6C7C316017F0870@genexch.genvec.com> References: <71CB4E18A03CB4448F2DD236C6C7C316017F0870@genexch.genvec.com> Message-ID: <48A43943.7070008@umdnj.edu> Hi Melissa: Tissue that thick must be sliced into 3-5 mm slices to get adequate fixation in the center. While an alcohol-based fixative such as formalin-alcohol-acetic acid will penetrate faster than buffered formalin, your tissue is too thick for adequate fixation in the center without slicing it into smaller pieces. Geoff Hamilton, Melissa wrote: > I am searching for a protocol for fixing mouse tumors (subcutaneously or > orthotopically grown) that are approx 2 to 2.5 cm in diameter. I am > having issues with penetration throughout the tissue. Thanks. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From meghan.tucker <@t> yahoo.com Thu Aug 14 09:19:20 2008 From: meghan.tucker <@t> yahoo.com (Meghan Tucker) Date: Thu Aug 14 09:19:23 2008 Subject: [Histonet] Auto coverslippers Message-ID: <163393.9080.qm@web54504.mail.re2.yahoo.com> Good Morning Everyone, ? I am looking into purchasing an auto coverslipper for our lab, does anyone have any suggestions? ? Thanks in advance! ? Meghan Tucker Biologist (Histotechnology) USDA ARS NAA PIADC Meghan.Tucker@yahoo.com Meghan.Tucker@ars.usda.gov ? From rjbuesa <@t> yahoo.com Thu Aug 14 09:24:21 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Aug 14 09:24:27 2008 Subject: [Histonet] Auto coverslippers In-Reply-To: <163393.9080.qm@web54504.mail.re2.yahoo.com> Message-ID: <664549.79818.qm@web65703.mail.ac4.yahoo.com> Buy Sakura. Ren? J. --- On Thu, 8/14/08, Meghan Tucker wrote: From: Meghan Tucker Subject: [Histonet] Auto coverslippers To: histonet@lists.utsouthwestern.edu Date: Thursday, August 14, 2008, 10:19 AM Good Morning Everyone, ? I am looking into purchasing an auto coverslipper for our lab, does anyone have any suggestions? ? Thanks in advance! ? Meghan Tucker Biologist (Histotechnology) USDA ARS NAA PIADC Meghan.Tucker@yahoo.com Meghan.Tucker@ars.usda.gov ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vsnider <@t> shrinenet.org Thu Aug 14 10:00:18 2008 From: vsnider <@t> shrinenet.org (Snider, Vivian Deanna) Date: Thu Aug 14 09:58:52 2008 Subject: [Histonet] Substitute Hematoxylin Dyes Message-ID: <84BE46B37B314D409C5A17B7BAB022D6023EF4E4@IDC-EX-VS01.shriners.cc> Dear Netters, What are you substituting for Hematoxylin? I have narrowed it down to 2 I want to research; The Tango Stain, from Anatech and Phoenix Blue Nuclear Stain, ordered from Fisher. Is anyone familiar with either of these stains? If so, what are the positive/negatives about each one? Are the results as comparable to the actual Hematoxylin dye? I have searched the net for images of a completed stain, to no avail. Any information will be greatly appreciated . Thanks in advance. Deanna Leslie HT ASCP Lead Histotechnician Histology Research Laboratory Shriners Hospital for Children Cincinnati, OH CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients. If you are not the intended recipient, (or authorized to receive for the recipient) you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please destroy all copies of this communication and any attachments and contact the sender by reply e-mail or telephone (813) 281-8100. From settembr <@t> umdnj.edu Thu Aug 14 10:16:26 2008 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Thu Aug 14 10:16:57 2008 Subject: [Histonet] Substitute Hematoxylin Dyes Message-ID: Hello Deanna, I can only tell you about how Phoenix Blue worked as a counterstain for immunos and it does not turn blue in the ammonia. It remained a lilac color which was difficult with the DAB. If the DAB staining was light on the patient case it was tough. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> "Snider, Vivian Deanna" 08/14/08 11:00 AM >>> Dear Netters, What are you substituting for Hematoxylin? I have narrowed it down to 2 I want to research; The Tango Stain, from Anatech and Phoenix Blue Nuclear Stain, ordered from Fisher. Is anyone familiar with either of these stains? If so, what are the positive/negatives about each one? Are the results as comparable to the actual Hematoxylin dye? I have searched the net for images of a completed stain, to no avail. Any information will be greatly appreciated . Thanks in advance. Deanna Leslie HT ASCP Lead Histotechnician Histology Research Laboratory Shriners Hospital for Children Cincinnati, OH CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients. If you are not the intended recipient, (or authorized to receive for the recipient) you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please destroy all copies of this communication and any attachments and contact the sender by reply e-mail or telephone (813) 281-8100. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ljones <@t> pathology.umsmed.edu Thu Aug 14 10:44:03 2008 From: ljones <@t> pathology.umsmed.edu (Linda Jones) Date: Thu Aug 14 10:44:19 2008 Subject: [Histonet] Has anyone been having problems with their alcohols while processing tissue? Message-ID: <48A40C730200004400028D11@GWIA1.umsmed.edu> Hi, My tissue appears to be dried out and the nuclei is hazy. Please help. Linda Harper-Jones BS.,HT/ HTL(ASCP) University of Mississippi Medical Center Department of Pathology Chief Histotechnologist Supervisor Telephone (601) 984-1576 Fax (601) 984-4968 ljones@pathology.umsmed.edu The information contained in this email is confidential. Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. From tjasper <@t> copc.net Thu Aug 14 11:16:10 2008 From: tjasper <@t> copc.net (Thomas Jasper) Date: Thu Aug 14 11:16:19 2008 Subject: [Histonet] Substitute Hematoxylin Dyes References: <84BE46B37B314D409C5A17B7BAB022D6023EF4E4@IDC-EX-VS01.shriners.cc> Message-ID: <90354A475B420441B2A0396E5008D4965E2147@copc-sbs.COPC.local> Hey Deanna, We tested the Phoenix Blue here...just in case. Pathologist feedback was basically - it works, I can read it if I need to, not as crisp as the H and E we run. Just curious...are you unable to obtain your regular hematoxylin? I was under the impression that this whole shortage thing was going to come to an end fairly soon. Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, OR 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Snider, Vivian Deanna Sent: Thursday, August 14, 2008 8:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Substitute Hematoxylin Dyes Dear Netters, What are you substituting for Hematoxylin? I have narrowed it down to 2 I want to research; The Tango Stain, from Anatech and Phoenix Blue Nuclear Stain, ordered from Fisher. Is anyone familiar with either of these stains? If so, what are the positive/negatives about each one? Are the results as comparable to the actual Hematoxylin dye? I have searched the net for images of a completed stain, to no avail. Any information will be greatly appreciated . Thanks in advance. Deanna Leslie HT ASCP Lead Histotechnician Histology Research Laboratory Shriners Hospital for Children Cincinnati, OH CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients. If you are not the intended recipient, (or authorized to receive for the recipient) you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please destroy all copies of this communication and any attachments and contact the sender by reply e-mail or telephone (813) 281-8100. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dnannenga <@t> incytepathology.com Thu Aug 14 11:35:29 2008 From: dnannenga <@t> incytepathology.com (Debra D. Nannenga) Date: Thu Aug 14 11:35:33 2008 Subject: [Histonet] P16 antibody Message-ID: <706224670091FE47997AEF88EFADE7CA09CE0E@EXCHANGE-SRV.PAI.E-PATHOLOGY.COM> Hello Histonetters, We have been using Biocare's P16 but I know that it is no longer available for sale. I am just wondering what other labs are using and how well they like it. Thanks to all who respond. Debbie Nannenga, HTL,QIHC InCyte Pathology Spokane Valley, Washington From micropathlabs <@t> yahoo.com Thu Aug 14 12:02:01 2008 From: micropathlabs <@t> yahoo.com (Sheila Haas) Date: Thu Aug 14 12:02:05 2008 Subject: [Histonet] Flow Cytometry in FL Message-ID: <89083.3195.qm@web57807.mail.re3.yahoo.com> Does anyone have any idea about the qualifications for performing flow cytometry in FL? Does it have to be a med tech performing test? Can?histology technologist perform test? I'm?looking?on the state web site but it's like looking for a needle in a haystack. Thanks in advance. ? Sheila Haas Laboratory Supervisor Micro Path Laboratories From kenneth.a.troutman <@t> Vanderbilt.Edu Thu Aug 14 12:10:19 2008 From: kenneth.a.troutman <@t> Vanderbilt.Edu (Troutman, Kenneth A) Date: Thu Aug 14 12:13:50 2008 Subject: [Histonet] P16 antibody Message-ID: <37DEF9AF72994947AF693956A59B9B660127FF71@mailbe03.mc.vanderbilt.edu> We use the MTM (CINtec) antibody. I don't particularly like it and we are looking into finding another ourselves. It comes in a kit (like DAKOs HercepTest) and I can say that it works, but it is dirty. Control tissue is difficult to find as well. Good luck and let us all know if you find a good one. I will do likewise. Ashley Troutman BS, HT(ASCP)QIHC Histopathology Laboratory Department of Pathology Vanderbilt University Medical Center Nashville, TN From mpence <@t> grhs.net Thu Aug 14 12:31:52 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Aug 14 12:32:03 2008 Subject: [Histonet] P16 antibody In-Reply-To: <37DEF9AF72994947AF693956A59B9B660127FF71@mailbe03.mc.vanderbilt.edu> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A38D7@IS-E2K3.grhs.net> Cell Marque has a p16 mouse available. Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Troutman, Kenneth A Sent: Thursday, August 14, 2008 12:10 PM To: Histonet Subject: [Histonet] P16 antibody We use the MTM (CINtec) antibody. I don't particularly like it and we are looking into finding another ourselves. It comes in a kit (like DAKOs HercepTest) and I can say that it works, but it is dirty. Control tissue is difficult to find as well. Good luck and let us all know if you find a good one. I will do likewise. Ashley Troutman BS, HT(ASCP)QIHC Histopathology Laboratory Department of Pathology Vanderbilt University Medical Center Nashville, TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tim.morken <@t> thermofisher.com Thu Aug 14 12:40:32 2008 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Thu Aug 14 12:41:05 2008 Subject: [Histonet] P16 antibody In-Reply-To: <37DEF9AF72994947AF693956A59B9B660127FF71@mailbe03.mc.vanderbilt.edu> References: <37DEF9AF72994947AF693956A59B9B660127FF71@mailbe03.mc.vanderbilt.edu> Message-ID: <6BFF6D137DF6BC43B33891BA96E83B1901BFCF7A@PGHCR-EXMB-VS-1.na.fshrnet.com> For p16, MTM labs is it. Under their patent they control all antibodies to p16 for diagnostic use. Tim Morken Technical Support Manager Lab Vision Products Anatomical Pathology ThermoFisher Scientific -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Troutman, Kenneth A Sent: Thursday, August 14, 2008 10:10 AM To: Histonet Subject: [Histonet] P16 antibody We use the MTM (CINtec) antibody. I don't particularly like it and we are looking into finding another ourselves. It comes in a kit (like DAKOs HercepTest) and I can say that it works, but it is dirty. Control tissue is difficult to find as well. Good luck and let us all know if you find a good one. I will do likewise. Ashley Troutman BS, HT(ASCP)QIHC Histopathology Laboratory Department of Pathology Vanderbilt University Medical Center Nashville, TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dzadory <@t> epl-inc.com Thu Aug 14 12:45:02 2008 From: dzadory <@t> epl-inc.com (Dan Zadory) Date: Thu Aug 14 12:42:19 2008 Subject: [Histonet] Sliding Microtome Taking Random Thicker Sections Message-ID: Hello, We have recently purchased an HM430 MICROM Sliding Microtome along with the KS 34 Fast Freezing Unit. Recently, I have tried sectioning frozen mouse brains at 40 microns and noticed after several cuts the Microtome will take a random thicker section (I would have to say approximately 150-200 microns thick). I am unsure as to why it keeps doing this. The knife is in the right position and all parts have been tightened. In addition, I have the Fast Freezing Unit set to keep the stage at a constant -22C. We placed a dehumidifier in the room since we thought the humidity may play a role...but so far no luck. Any suggestions? Daniel Zadory From mwich <@t> 7thwavelabs.com Thu Aug 14 12:49:34 2008 From: mwich <@t> 7thwavelabs.com (Michele Wich) Date: Thu Aug 14 12:49:39 2008 Subject: [Histonet] caspase-3 Message-ID: <62A8156F8071C8439080D626DF8C33A602E477@wave-mail.7thwave.local> A couple questions for those of you doing caspase-3 on animal tissue: Is it necessary to run a negative control tissue? What is a good positive control tissue? Will mouse ovary/uterus work? Thanks in advance for any help with this! This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer From Heather.D.Renko <@t> osfhealthcare.org Thu Aug 14 12:53:10 2008 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Thu Aug 14 12:54:09 2008 Subject: [Histonet] Re: coverslipper Message-ID: <40026EDDE64CDA47AB382C52619ACD3C0A09E0DB@pmc-rfd-mx01.intranet.osfnet.org> We love the Sakura tape cover slipper! Great company, great products, great service. Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From liz <@t> premierlab.com Thu Aug 14 12:54:26 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Aug 14 12:54:30 2008 Subject: [Histonet] caspase-3 In-Reply-To: <62A8156F8071C8439080D626DF8C33A602E477@wave-mail.7thwave.local> References: <62A8156F8071C8439080D626DF8C33A602E477@wave-mail.7thwave.local> Message-ID: We use mouse mesenteric lymph node as a positive control. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele Wich Sent: Thursday, August 14, 2008 11:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] caspase-3 A couple questions for those of you doing caspase-3 on animal tissue: Is it necessary to run a negative control tissue? What is a good positive control tissue? Will mouse ovary/uterus work? Thanks in advance for any help with this! This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lia.Caldwell <@t> TriadHospitals.com Thu Aug 14 13:04:33 2008 From: Lia.Caldwell <@t> TriadHospitals.com (Caldwell, Lia) Date: Thu Aug 14 13:08:49 2008 Subject: [Histonet] Substitute Hematoxylin Dyes References: Message-ID: <774F7DC732C89743BB070F1E87D402541A8197@TNTRIEXEVS04.triadhospitals.net> We tested the Phoenix Blue with our Pathologists and the general consensus was that it was far inferior to the H/E stain. Our pathologists are used to a regressive H/E and Phoenix Blue can only be run progressively. The pathologists seemed troubled with the nuclear detail provided by Phx Blue particularly in Gi biopsies (which is the majority of our volumes). We will be testing Tango next - I will keep you posted if you are interested. ~Lia Lia M. Caldwell HT (ASCP) Histology Supervisor Oro Valley Pathology Dept. phone: (520) 901-3914 www.Lia.Caldwell@TriadHospitals.com "be yourself - everyone else is already taken." -unknown ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Dana Settembre Sent: Thu 8/14/2008 8:16 AM To: histonet@lists.utsouthwestern.edu; Vivian Deanna Snider Subject: Re: [Histonet] Substitute Hematoxylin Dyes Hello Deanna, I can only tell you about how Phoenix Blue worked as a counterstain for immunos and it does not turn blue in the ammonia. It remained a lilac color which was difficult with the DAB. If the DAB staining was light on the patient case it was tough. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> "Snider, Vivian Deanna" 08/14/08 11:00 AM >>> Dear Netters, What are you substituting for Hematoxylin? I have narrowed it down to 2 I want to research; The Tango Stain, from Anatech and Phoenix Blue Nuclear Stain, ordered from Fisher. Is anyone familiar with either of these stains? If so, what are the positive/negatives about each one? Are the results as comparable to the actual Hematoxylin dye? I have searched the net for images of a completed stain, to no avail. Any information will be greatly appreciated . Thanks in advance. Deanna Leslie HT ASCP Lead Histotechnician Histology Research Laboratory Shriners Hospital for Children Cincinnati, OH CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients. If you are not the intended recipient, (or authorized to receive for the recipient) you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please destroy all copies of this communication and any attachments and contact the sender by reply e-mail or telephone (813) 281-8100. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From mpence <@t> grhs.net Thu Aug 14 13:19:05 2008 From: mpence <@t> grhs.net (Mike Pence) Date: Thu Aug 14 13:19:09 2008 Subject: [Histonet] Re: coverslipper In-Reply-To: <40026EDDE64CDA47AB382C52619ACD3C0A09E0DB@pmc-rfd-mx01.intranet.osfnet.org> Message-ID: <661949901A768E4F9CC16D8AF8F2838C017A38D9@IS-E2K3.grhs.net> I would not buy anything but the Sakura tape! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Renko, Heather D. Sent: Thursday, August 14, 2008 12:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: coverslipper We love the Sakura tape cover slipper! Great company, great products, great service. Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ======================================================================== ====== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Aug 14 13:30:42 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Aug 14 13:30:45 2008 Subject: [Histonet] Flow Cytometry in FL In-Reply-To: <89083.3195.qm@web57807.mail.re3.yahoo.com> Message-ID: <930501.57691.qm@web65711.mail.ac4.yahoo.com> USUALLY, meaning that?there can be exceptions, Flow Cytometry had "drifted" from histology to hematology and in those cases an MT is in charge of performing the tests to be read by a pathologist (all in Florida). Ren? J. --- On Thu, 8/14/08, Sheila Haas wrote: From: Sheila Haas Subject: [Histonet] Flow Cytometry in FL To: histonet@lists.utsouthwestern.edu Date: Thursday, August 14, 2008, 1:02 PM Does anyone have any idea about the qualifications for performing flow cytometry in FL? Does it have to be a med tech performing test? Can?histology technologist perform test? I'm?looking?on the state web site but it's like looking for a needle in a haystack. Thanks in advance. ? Sheila Haas Laboratory Supervisor Micro Path Laboratories _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Aug 14 13:34:12 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Aug 14 13:34:18 2008 Subject: [Histonet] Sliding Microtome Taking Random Thicker Sections In-Reply-To: Message-ID: <1191.76996.qm@web65703.mail.ac4.yahoo.com> Even if everything as you say is "tight", check the feeding mechanism (the connection screw/vertical advance of the block holder). Ren? J. --- On Thu, 8/14/08, Dan Zadory wrote: From: Dan Zadory Subject: [Histonet] Sliding Microtome Taking Random Thicker Sections To: histonet@lists.utsouthwestern.edu Date: Thursday, August 14, 2008, 1:45 PM Hello, We have recently purchased an HM430 MICROM Sliding Microtome along with the KS 34 Fast Freezing Unit. Recently, I have tried sectioning frozen mouse brains at 40 microns and noticed after several cuts the Microtome will take a random thicker section (I would have to say approximately 150-200 microns thick). I am unsure as to why it keeps doing this. The knife is in the right position and all parts have been tightened. In addition, I have the Fast Freezing Unit set to keep the stage at a constant -22C. We placed a dehumidifier in the room since we thought the humidity may play a role...but so far no luck. Any suggestions? Daniel Zadory _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From detmar <@t> mshri.on.ca Thu Aug 14 13:46:35 2008 From: detmar <@t> mshri.on.ca (Jacqui Detmar) Date: Thu Aug 14 13:46:50 2008 Subject: [Histonet] caspase-3 In-Reply-To: References: <62A8156F8071C8439080D626DF8C33A602E477@wave-mail.7thwave.local> Message-ID: Hi Michele. I use mouse thymus or spleen controls. I've done caspase-3 IHC on mouse placenta/uterus and you'll get signals, but the problem with that tissue is that you tend to have different types of cell death going on (i.e. non-canonical cell death pathways are triggered that don't use caspase-3), so it can be confusing to interpret the results. Also, we have found that active caspase-3 is utilized for signaling pathways other than cell death, so you might see positives that will likewise, be confusing. Mouse lymphocytes die a nice, normal death so using thymus or spleen will give you unambiguous results. Good luck and if you have any further questions, feel free to ask! Jacqui Jacqui Detmar, Post-doctoral Fellow Samuel Lunenfeld Research Institute, Mount Sinai Hospital 25 Orde Street, room 6-1001 AJ, Toronto, ON, Canada M5T 3H7 phone: 416-586-4800 x5607 fax: 416-586-8588 email: detmar@mshri.on.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele Wich Sent: Thursday, August 14, 2008 11:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] caspase-3 A couple questions for those of you doing caspase-3 on animal tissue: Is it necessary to run a negative control tissue? What is a good positive control tissue? Will mouse ovary/uterus work? Thanks in advance for any help with this! From LChen <@t> mednet.ucla.edu Thu Aug 14 13:48:18 2008 From: LChen <@t> mednet.ucla.edu (Chen, Leslie) Date: Thu Aug 14 13:48:23 2008 Subject: [Histonet] Good Cresyl Violet Recipe References: <200808141705.m7EH5b1b023257@proofpoint3.medctr.ucla.edu> Message-ID: <9F8AE7E7B303F44DB0CAB587F1E96C3F08FB35@admedmail3.ad.medctr.ucla.edu> Hi Everyone, My post-doc is asking me for a good cresyl violet recipe. Does anyone have one to share? Thanks! Leslie Chen ---------------------------------------------------------- IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. ---------------------------------------------------------- From jqb7 <@t> cdc.gov Thu Aug 14 13:57:44 2008 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Thu Aug 14 14:01:34 2008 Subject: [Histonet] Re: coverslipper In-Reply-To: <661949901A768E4F9CC16D8AF8F2838C017A38D9@IS-E2K3.grhs.net> References: <40026EDDE64CDA47AB382C52619ACD3C0A09E0DB@pmc-rfd-mx01.intranet.osfnet.org> <661949901A768E4F9CC16D8AF8F2838C017A38D9@IS-E2K3.grhs.net> Message-ID: <1CE1847DFEA0A647B1CCDE4108EA60A70208BFC3@LTA3VS011.ees.hhs.gov> We have had great results with the Leica glass coverslipper....we cannot use tape. But I hear that Sakura has a really great glass coverslipper as well. I would go with glass if possible. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Thursday, August 14, 2008 2:19 PM To: Renko, Heather D.; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: coverslipper I would not buy anything but the Sakura tape! Mike -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Renko, Heather D. Sent: Thursday, August 14, 2008 12:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: coverslipper We love the Sakura tape cover slipper! Great company, great products, great service. Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ======================================================================== ====== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Thu Aug 14 14:45:13 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Aug 14 14:45:19 2008 Subject: [Histonet] Glypican 3 and Pax 5 Message-ID: <982A0A9461F9BF438C7B19A6E425A3834E4920@ITSSSXM01V6.one.ads.che.org> I need vendor recommendations for these two antibodies please. Thanks! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From gmartin <@t> marshallmedical.org Thu Aug 14 13:39:29 2008 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Thu Aug 14 14:46:25 2008 Subject: [Histonet] Digital voice recorder Message-ID: <6ED9D4252F278841A0593D3D788AF24C0310912D@mailsvr.MARSHMED.local> We have been using a Phillips 730 dictation system (mini cassettes) for ever and now we need to replace one of the units. It has become obvious that this is old technology, so we would like to move on to a digital voice recorder. I would appreciate any recommendation the Histo group could pass along. Thanks Gary From Sandra.Etheridge <@t> gov.bc.ca Thu Aug 14 16:47:56 2008 From: Sandra.Etheridge <@t> gov.bc.ca (Etheridge, Sandra AL:EX) Date: Thu Aug 14 16:48:05 2008 Subject: [Histonet] DAB Waste Neutralization Procedure Message-ID: Hello, everyone, I was wondering if anyone has a procedure and reference for the DAB waste neutralization procedure using 30% hydrogen peroxide and the horseradish peroxidase? Any info is appreciated. Thanks, Sandra Etheridge BC Ministry of Agriculture & Lands Animal Health Center, Histology/IHC Abbotsford, BC Canada From lpwenk <@t> sbcglobal.net Thu Aug 14 20:30:56 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Aug 14 20:31:09 2008 Subject: [Histonet] Good Cresyl Violet Recipe In-Reply-To: <9F8AE7E7B303F44DB0CAB587F1E96C3F08FB35@admedmail3.ad.medctr.ucla.edu> Message-ID: <000301c8fe76$90f17b40$0202a8c0@HPPav2> Does the post-doc mean cresyl violet acetate, formerly known at cresyl echt violet? What is the post-doc staining for? Nissl bodies? Pneumocystis? Nuclei? Mast cell granules? That would determine the exact recipe and procedure. Sorry - asking for a recipe for a dye is like asking - does anyone have a hematoxylin recipe, to which people will say, do you want blue or black nuclei (Mayer or Gill hematoxylin vs. Weigert)? Do you want to to stain elastin (VVG)? Do you want to stain myelin (Weil)? All these stain with hematoxylin, but each one has a different procedure to make up the hematoxylin stain, and to use it. Same with your cresyl violet. Sorry, need a little bit more information before we can help. Peggy A. Wenk HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chen, Leslie Sent: Thursday, August 14, 2008 2:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Good Cresyl Violet Recipe Hi Everyone, My post-doc is asking me for a good cresyl violet recipe. Does anyone have one to share? Thanks! Leslie Chen ---------------------------------------------------------- IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. ---------------------------------------------------------- From mikael.niku <@t> helsinki.fi Fri Aug 15 02:13:22 2008 From: mikael.niku <@t> helsinki.fi (Mikael Niku) Date: Fri Aug 15 02:13:30 2008 Subject: [Histonet] Murine leukocytes on paraffin? Message-ID: <005901c8fea6$66ddd760$97a5d680@mmkem12636> Dear Histonetters, I'm looking for antibodies to identify basic leukocyte types on murine PFA-fixed paraffin sections (such as tissue macrophages, B cells, and CD4+CD8). I just noticed that AbD Serotec seems to offer a fairly nice range of rat and hamster monoclonals for these purposes. Any experience on these products, do they really work well on paraffin? With best regards, Mikael ----------------------------------------------------- Mikael Niku, PhD, university lecturer University of Helsinki, Finland URL: mikael.nikunnakki.info - What do I think of western civilization? I think it would be a good idea! Gandhi ----------------------------------------------------- From louise.renton <@t> gmail.com Fri Aug 15 02:41:15 2008 From: louise.renton <@t> gmail.com (louise renton) Date: Fri Aug 15 02:41:20 2008 Subject: [Histonet] Freezing Haematoxylin Message-ID: Dear all, Here's a question to tickle your brain cells before the weekend Can I freeze aliquots of Mayer's Haem (home made) for later use? Would there be any negative effects on composition/staining etc??? -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From TMcNemar <@t> lmhealth.org Fri Aug 15 06:12:01 2008 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Aug 15 06:11:49 2008 Subject: [Histonet] Documenting control tissue source? Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F5AD@lmhsmail.lmhealth.org> We are finishing up our JCAHO inspection today. The inspector asked about our control tissues and where we documented the source. We get nearly all of our controls from patient samples and she said that most histo labs mark their control blocks with the source case number. They then keep records of the control source used when staining new cases. I can see no logical reason to keep this info. You use a control appropriate for the element being stained. Why does it matter where the control came from? Am I wrong? Do you do this? Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org From AFeatherstone <@t> KaleidaHealth.Org Fri Aug 15 07:36:48 2008 From: AFeatherstone <@t> KaleidaHealth.Org (Featherstone, Annette) Date: Fri Aug 15 07:36:56 2008 Subject: [Histonet] Cryostat, Cassette Labeller In-Reply-To: References: Message-ID: <16A5E67B2A1F714885DEDC2CB68DD6091D7B7C@KALEXMB03.KaleidaHealth.org> Can anyone recommend a cryostat that they are currently using and that all your staff loves? Also we are looking for a new cassette labeler. We are currently using Thermo Shandon and having a lot of problems. Any suggestions would be appreciated. Thanks Annette, Kaleida Health, Buffalo General 2007 Best Places to Work Finalist Visit our careers page at www.kaleidahealth.org/careers CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From Lynn.Burton <@t> Illinois.gov Fri Aug 15 08:08:19 2008 From: Lynn.Burton <@t> Illinois.gov (Burton, Lynn) Date: Fri Aug 15 08:09:15 2008 Subject: [Histonet] Re: coverslipper References: <40026EDDE64CDA47AB382C52619ACD3C0A09E0DB@pmc-rfd-mx01.intranet.osfnet.org> Message-ID: Ditto on the tape cover slipper from Sakura here. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Renko, Heather D. Sent: Thu 8/14/2008 12:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: coverslipper We love the Sakura tape cover slipper! Great company, great products, great service. Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From microwave <@t> ebsciences.com Fri Aug 15 08:11:45 2008 From: microwave <@t> ebsciences.com (Tracy Cloyd) Date: Fri Aug 15 08:12:01 2008 Subject: [Histonet] Microwave fixation of whole organs Message-ID: <48A58091.2060500@ebsciences.com> Hello all, Is anyone fixing whole organs (prostates, placentas) in the microwave? If so, what is your procedure? I have heard of fixing a whole prostate for 5 minutes at 65?. Seems like a short time. Thoughts? -- Regards, Tracy From rjbuesa <@t> yahoo.com Fri Aug 15 08:36:59 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 15 08:37:03 2008 Subject: [Histonet] Freezing Haematoxylin In-Reply-To: Message-ID: <55735.67168.qm@web65705.mail.ac4.yahoo.com> Louise: You just stepped into "uncharted waters" because except for now when there seems to be some hematoxylin shortage, I think that what you ask was never tried before. So, as a present day Magellan, why don't you do the experiment and pass to all of us the results? Ren? J. --- On Fri, 8/15/08, louise renton wrote: From: louise renton Subject: [Histonet] Freezing Haematoxylin To: Histonet@lists.utsouthwestern.edu Date: Friday, August 15, 2008, 3:41 AM Dear all, Here's a question to tickle your brain cells before the weekend Can I freeze aliquots of Mayer's Haem (home made) for later use? Would there be any negative effects on composition/staining etc??? -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Aug 15 08:40:46 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 15 08:40:52 2008 Subject: [Histonet] Documenting control tissue source? In-Reply-To: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F5AD@lmhsmail.lmhealth.org> Message-ID: <455827.97250.qm@web65701.mail.ac4.yahoo.com> Never done that. Typically a control (either + or -) will react as it is supposed to, REGARDLESS of its source, which is mostly?irrelevant. As to the rationale of why doing that, you missed the opportunity of asking that to the "pencil/paper pusher" who inspected you! Ren? J. --- On Fri, 8/15/08, Tom McNemar wrote: From: Tom McNemar Subject: [Histonet] Documenting control tissue source? To: histonet@pathology.swmed.edu Date: Friday, August 15, 2008, 7:12 AM We are finishing up our JCAHO inspection today. The inspector asked about our control tissues and where we documented the source. We get nearly all of our controls from patient samples and she said that most histo labs mark their control blocks with the source case number. They then keep records of the control source used when staining new cases. I can see no logical reason to keep this info. You use a control appropriate for the element being stained. Why does it matter where the control came from? Am I wrong? Do you do this? Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Aug 15 08:45:13 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 15 08:45:16 2008 Subject: [Histonet] Microwave fixation of whole organs In-Reply-To: <48A58091.2060500@ebsciences.com> Message-ID: <83455.93351.qm@web65714.mail.ac4.yahoo.com> Fixing whole organs in the microwave is the wrong practice. Any organ heated above 50?C will determine that the proteins will be HEAT COAGULATED and that is absolutely other thing to fixing. In the irresponsible quest for speed, by yielding to administrative pressures to discharge patients sooner than required and cut costs, we sometimes are ruining the tissues we have to work with?later on. Very bad practice! Ren? J. --- On Fri, 8/15/08, Tracy Cloyd wrote: From: Tracy Cloyd Subject: [Histonet] Microwave fixation of whole organs To: histonet@lists.utsouthwestern.edu Date: Friday, August 15, 2008, 9:11 AM Hello all, Is anyone fixing whole organs (prostates, placentas) in the microwave? If so, what is your procedure? I have heard of fixing a whole prostate for 5 minutes at 65?. Seems like a short time. Thoughts? -- Regards, Tracy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Aug 15 08:55:05 2008 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Aug 15 08:55:10 2008 Subject: [Histonet] Microwave fixation of whole organs Message-ID: <86ADE4EB583CE64799A9924684A0FBBF05005E7F@wahtntex2.waht.swest.nhs.uk> I regularly fix whole organs in the microwave usually muscle. The secret is to make sure that you don't microwave too quickly and that you make sure you catch the juices. This can be used to make nice gravy to pour over it. Micro-waved fish biopsies coated with breadcrumbs are good but I can't get cured pig skin to become crispy. Kemlo Rogerson Pathology Manager This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From leiker <@t> buffalo.edu Fri Aug 15 09:17:45 2008 From: leiker <@t> buffalo.edu (Merced Leiker) Date: Fri Aug 15 09:17:49 2008 Subject: [Histonet] Microwave fixation of whole organs In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBF05005E7F@wahtntex2.waht.swest.nhs.uk> References: <86ADE4EB583CE64799A9924684A0FBBF05005E7F@wahtntex2.waht.swest.n hs.uk> Message-ID: <20C44437807017C0B911B01F@bchwxp2702.ad.med.buffalo.edu> LOL! My Friday cure for the blues.... (no pun intended....!) --On Friday, August 15, 2008 2:55 PM +0100 Kemlo Rogerson wrote: > I regularly fix whole organs in the microwave usually muscle. The secret > is to make sure that you don't microwave too quickly and that you make > sure you catch the juices. This can be used to make nice gravy to pour > over it. > > Micro-waved fish biopsies coated with breadcrumbs are good but I can't > get cured pig skin to become crispy. > > > Kemlo Rogerson > Pathology Manager > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. > Please inform me that this message has gone astray before deleting it. > Thank you for your co-operation > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Merced M Leiker Research Technician II 354 BRB (Lee Lab) / 140 Farber Hall (mail) School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 In order to put yourself in someone else's shoes, you must first take off yours. From sbreeden <@t> nmda.nmsu.edu Fri Aug 15 09:31:35 2008 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Aug 15 09:31:39 2008 Subject: [Histonet] Coverslippers and Hematoxylin and OT Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E646B@nmdamailsvr.nmda.ad.nmsu.edu> Tape coverslipper - I use the KP brand tape (sold by Mercedes Medical) on my Sakura and it works exactly like the name brand and is a longer roll for less money. In the dry NM air, I just make sure that there are 3-4 drops of xylene (no substitutes!) per slide and I have no lifting, no drying and I can file them immediately. Hematoxylin - I really recommend you try the Surgipath SelecTech H&E system! My pathologists (3) love it and the hematoxylin is good for something like 2500 slides - the eosin is good for 1500 slides. Long shelf life (about 2 years), very economical and the staining is exceptional. This IS Friday, so send your donations to my Wealthy Medical Dudette ("WMD") Fund in any amount over $10. I have 2 years to get enough money for that beach house in Costa Rica with internet connection! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From gayle.callis <@t> bresnan.net Fri Aug 15 10:05:39 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Fri Aug 15 10:05:46 2008 Subject: [Histonet] Murine leukocytes on paraffin? References: <005901c8fea6$66ddd760$97a5d680@mmkem12636> Message-ID: <000e01c8fee8$619a0250$6401a8c0@Sunney> CD4 and CD8 will never work on Paraformaldehyde fixed, paraffin embedded murinE tissue no matter what retrieval OR enzyme digestion you try. Sad, but true! Macrophage antibody, F4/80 will work with retrieval (in histonet archives several times) and I am not sure about B220. We only do frozen sections on fresh snap frozen tissues, fixed with 25% absolute ethanol/75% acetone, a RT fixation on thoroughly air dried frozen sections (overnight). If you want to stain adjacent sections for CD4, CD8, F4/80, and B220 - then you would have to do cryotomy. Only one of these four antibodies will work on FFPE or PFAF-PE murine tissue. Serotec has a good range of murine monoclonals, but we purchase most of our murine antibodies from eBioscience or Invitrogen (BD Pharmingen). There is a non-aldehyde fixative you can purchase from BD Pharmingen i.e. Invitrogen that they call zinc fixative. It is FREE of formalin and paraformaldehyde, you can make it up yourself. We refer to this formalin free fixative as Zinc TRIS. You can go J Histochemistry and Cytochemistry, for a publication by Jay Beckstead. He developed this fixative for human lymphoma CD marker staining and it was then published for use with rodent tissue H. Nitta et al, in a now defunct journal called Cell Vision. Whatever you do, do NOT confuse Zinc TRIS buffer as zinc formalin as the latter will not work. This fixative was recently discussed on Histonet (last month). If you go to the archives you can probably pick up the recipe and further discussion. Gayle M. Callis HTL/HT/MT(ASCP) ----- Original Message ----- From: "Mikael Niku" To: Sent: Friday, August 15, 2008 1:13 AM Subject: [Histonet] Murine leukocytes on paraffin? > Dear Histonetters, > > I'm looking for antibodies to identify basic leukocyte types on murine > PFA-fixed paraffin sections (such as tissue macrophages, B cells, and > CD4+CD8). > > I just noticed that AbD Serotec seems to offer a fairly nice range of rat > and hamster monoclonals for these purposes. Any experience on these > products, do they really work well on paraffin? > > With best regards, > Mikael > > ----------------------------------------------------- > Mikael Niku, PhD, university lecturer > University of Helsinki, Finland > URL: mikael.nikunnakki.info > > - What do I think of western civilization? > I think it would be a good idea! > Gandhi > ----------------------------------------------------- > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Fri Aug 15 10:11:01 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Fri Aug 15 10:11:05 2008 Subject: [Histonet] Cryostat, Cassette Labeller References: <16A5E67B2A1F714885DEDC2CB68DD6091D7B7C@KALEXMB03.KaleidaHealth.org> Message-ID: <001c01c8fee9$20d675e0$6401a8c0@Sunney> Leica Cryocuts, two models, we have three 1850's and love them. These are used in research setting and also popular for clinical use also. Look at the new 1950, it has some newer features ( i.e.a brush technique application with removable platform) that are very nice and it can be purchased as either manual or automated units. ----- Original Message ----- From: "Featherstone, Annette" To: Sent: Friday, August 15, 2008 6:36 AM Subject: [Histonet] Cryostat, Cassette Labeller Can anyone recommend a cryostat that they are currently using and that all your staff loves? Also we are looking for a new cassette labeler. We are currently using Thermo Shandon and having a lot of problems. Any suggestions would be appreciated. Thanks Annette, Kaleida Health, Buffalo General 2007 Best Places to Work Finalist Visit our careers page at www.kaleidahealth.org/careers CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Fri Aug 15 10:16:51 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Fri Aug 15 10:16:56 2008 Subject: [Histonet] Freezing Haematoxylin References: <55735.67168.qm@web65705.mail.ac4.yahoo.com> Message-ID: <002601c8fee9$f1f9bc90$6401a8c0@Sunney> We had Gill hematoxylins sent to us in winter and the stain froze during shipment. This totally ruined the stain, and was an expensive disaster. If you look at a Gill's recipe and Mayers, they are similar. Since then, all ordering for hematoxylin in now done in warmer months. Care is now taken to not freeze any hematoxylins. Gayle M. Callis HTL/HT/MT(ASCP) ----- Original Message ----- From: "Rene J Buesa" To: ; "louise renton" Sent: Friday, August 15, 2008 7:36 AM Subject: Re: [Histonet] Freezing Haematoxylin Louise: You just stepped into "uncharted waters" because except for now when there seems to be some hematoxylin shortage, I think that what you ask was never tried before. So, as a present day Magellan, why don't you do the experiment and pass to all of us the results? Ren? J. --- On Fri, 8/15/08, louise renton wrote: From: louise renton Subject: [Histonet] Freezing Haematoxylin To: Histonet@lists.utsouthwestern.edu Date: Friday, August 15, 2008, 3:41 AM Dear all, Here's a question to tickle your brain cells before the weekend Can I freeze aliquots of Mayer's Haem (home made) for later use? Would there be any negative effects on composition/staining etc??? -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWeems <@t> sjha.org Fri Aug 15 11:07:51 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Aug 15 11:07:55 2008 Subject: [Histonet] Freezing Haematoxylin In-Reply-To: References: Message-ID: <982A0A9461F9BF438C7B19A6E425A3834E4B70@ITSSSXM01V6.one.ads.che.org> I don't think so... If hematoxylin freezes in shipping, it crystallizes and must be replaced. My 2 cents worth... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: Friday, August 15, 2008 3:41 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Freezing Haematoxylin Dear all, Here's a question to tickle your brain cells before the weekend Can I freeze aliquots of Mayer's Haem (home made) for later use? Would there be any negative effects on composition/staining etc??? -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From tim.morken <@t> thermofisher.com Fri Aug 15 11:11:55 2008 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Fri Aug 15 11:20:41 2008 Subject: [Histonet] Documenting control tissue source? In-Reply-To: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F5AD@lmhsmail.lmhealth.org> References: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F5AD@lmhsmail.lmhealth.org> Message-ID: <6BFF6D137DF6BC43B33891BA96E83B1901C669B8@PGHCR-EXMB-VS-1.na.fshrnet.com> Tom, I think it is a good practice, but not sure about regulations besides privacy. It would be good to get away from the old way of using anonomous tissue (that is, you can't trace it back to a specific patient and disease). But it depends on what you need to document. There is value in documenting the original source so you can trace back when you need to understand the disease, fixation/processing or other results that may be pertinent to your needs. It would be very helpful to know if it was processed in your lab or a "foreign" lab that may have different procedures. There may be some legal issues as well concerning patinet permission to use the tissue, in which case documentation is essential. If you don't want to use the original number on all your paperwork (say, for privacy or security issues) you can set up a master list that has the original number and then key to a new "control tissue number" that you use in daily work. Tim Morken Technical Support Manager Lab Vision Products Anatomical Pathology ThermoFisher Scientific -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Friday, August 15, 2008 4:12 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Documenting control tissue source? We are finishing up our JCAHO inspection today. The inspector asked about our control tissues and where we documented the source. We get nearly all of our controls from patient samples and she said that most histo labs mark their control blocks with the source case number. They then keep records of the control source used when staining new cases. I can see no logical reason to keep this info. You use a control appropriate for the element being stained. Why does it matter where the control came from? Am I wrong? Do you do this? Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Fri Aug 15 11:21:54 2008 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Fri Aug 15 11:21:57 2008 Subject: [Histonet] Perl's iron stain + IF together Message-ID: <930751.84017.qm@web50302.mail.re2.yahoo.com> Hello, Has anyone ever done a Perl's iron stain in conjunction with IF staining for?CD markers?? I am not sure?which I?would need to do first or if one would mask the other. Any thoughts? Kim ?Kim Merriam, MA, HT(ASCP) Cambridge, MA From liz <@t> premierlab.com Fri Aug 15 11:26:45 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Aug 15 11:26:54 2008 Subject: [Histonet] Perl's iron stain + IF together In-Reply-To: <930751.84017.qm@web50302.mail.re2.yahoo.com> References: <930751.84017.qm@web50302.mail.re2.yahoo.com> Message-ID: Kim I'm not sure about IF but you can do perls with routine IHC with either DAB or AP. If you are going to use DAB stain the IHC first then stain the perls. If you are doing AP detection with fast red then stain with perls first and then perform the IHC. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Friday, August 15, 2008 10:22 AM To: Histonet Subject: [Histonet] Perl's iron stain + IF together Hello, Has anyone ever done a Perl's iron stain in conjunction with IF staining for?CD markers?? I am not sure?which I?would need to do first or if one would mask the other. Any thoughts? Kim ?Kim Merriam, MA, HT(ASCP) Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tjasper <@t> copc.net Fri Aug 15 11:41:42 2008 From: tjasper <@t> copc.net (Thomas Jasper) Date: Fri Aug 15 11:41:49 2008 Subject: [Histonet] Documenting control tissue source? References: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F5AD@lmhsmail.lmhealth.org> Message-ID: <90354A475B420441B2A0396E5008D4965E214E@copc-sbs.COPC.local> Hey Tom, Keeping some patient accession numbers for tapping the control source later on could be handy. But, I've never heard of it as a mandatory policy or a regulatory compliance issue. Besides, new control tissue, from your patient source can be obtained by an LIS search. To flip this around, from a regulatory standpoint...it's possible that HIPAA could take a dim view re: patient privacy. I mean we work with patient numbers all the time, but if it's not necessary (for controls) why go there? It's possible that someone (the inspector) may have been trying to "mold" your service into their vision of what labs ought to be, as opposed to just inspecting you and making objective evaluations based on regs. I've seen this more than once with CAP inspectors (I realize this was JCAHO). Someone may have been blowing smoke up your...here. Where you shown any documentation to this effect? I don't think you're doing anything wrong. And for her to tell you that most histo labs do this was a bit presumptuous. Tom J. Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, OR 97701 541/693-2677 tjasper@copc.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Friday, August 15, 2008 4:12 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Documenting control tissue source? We are finishing up our JCAHO inspection today. The inspector asked about our control tissues and where we documented the source. We get nearly all of our controls from patient samples and she said that most histo labs mark their control blocks with the source case number. They then keep records of the control source used when staining new cases. I can see no logical reason to keep this info. You use a control appropriate for the element being stained. Why does it matter where the control came from? Am I wrong? Do you do this? Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcarpenter764 <@t> aol.com Fri Aug 15 11:46:07 2008 From: jcarpenter764 <@t> aol.com (jcarpenter764@aol.com) Date: Fri Aug 15 11:46:29 2008 Subject: [Histonet] CLIA Regulation for the Histology laboratory. Message-ID: <8CACD0D8C4EE2D8-7D4-1CF0@WEBMAIL-MB01.sysops.aol.com> Hello histoneers, I'm trying to find the exact regulations governed by CLIA specifically for histology. I was trying to find a website, but keep being redirected. If anyone could send me some information that would be helpful. Thanks. Jennell From hstevens3 <@t> mail.gatech.edu Fri Aug 15 11:47:48 2008 From: hstevens3 <@t> mail.gatech.edu (Hazel Stevens) Date: Fri Aug 15 11:47:55 2008 Subject: [Histonet] visualization of blood vessels in MMA embedded samples Message-ID: <006f01c8fef6$a6dd9ad0$f498d070$@gatech.edu> Hi Histonet, Does anyone know of a staining procedure which will pick out blood vessels in MMA embedded rat bone/cartilage samples. Is it necessary to get rid of the plastic ??? Any help greatly appreciated. Thanks Hazel Hazel Stevens, Temporary Research Scientist I, George W. Woodruff School of Mechanical Engineering, 315 Ferst Drive, IBB 2420, Atlanta, Georgia 30332-0405 U.S.A. E-mail: hazel.stevens@me.gatech.edu WEB: http://www.me.gatech.edu From rjbuesa <@t> yahoo.com Fri Aug 15 12:08:25 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 15 12:08:29 2008 Subject: [Histonet] Freezing Haematoxylin In-Reply-To: <982A0A9461F9BF438C7B19A6E425A3834E4B70@ITSSSXM01V6.one.ads.che.org> Message-ID: <681151.22568.qm@web65711.mail.ac4.yahoo.com> Ah, the things you learn when living in the "cold area" of the country! Ren? J. --- On Fri, 8/15/08, Weems, Joyce wrote: From: Weems, Joyce Subject: RE: [Histonet] Freezing Haematoxylin To: "louise renton" , Histonet@lists.utsouthwestern.edu Date: Friday, August 15, 2008, 12:07 PM I don't think so... If hematoxylin freezes in shipping, it crystallizes and must be replaced. My 2 cents worth... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: Friday, August 15, 2008 3:41 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Freezing Haematoxylin Dear all, Here's a question to tickle your brain cells before the weekend Can I freeze aliquots of Mayer's Haem (home made) for later use? Would there be any negative effects on composition/staining etc??? -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Fri Aug 15 12:21:00 2008 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Fri Aug 15 12:21:04 2008 Subject: [Histonet] Perl's iron stain + IF together Message-ID: <200899.97797.qm@web50102.mail.re2.yahoo.com> Perl's will turn DAB black and vice?versa. It works like using nickel to enhance DAB. ?Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 631 N. Broadway Ave. Moore, OK 73160 405-570-6679 cell 405-759-3953 lab contact@excaliburpathology.com www.excaliburpathology.com From Kurtz.Mathew <@t> mayo.edu Fri Aug 15 12:26:50 2008 From: Kurtz.Mathew <@t> mayo.edu (Kurtz, Mathew) Date: Fri Aug 15 12:27:59 2008 Subject: [Histonet] Freezing Haematoxylin References: <55735.67168.qm@web65705.mail.ac4.yahoo.com> Message-ID: <0B8BA0705C5073449141EB5F44CF957001CA563E@LMMAILVS1.ad.lmmhs.org> I agree!!! Be the tip of the sword!!! ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Fri 8/15/2008 8:36 AM To: Histonet@lists.utsouthwestern.edu; louise renton Subject: Re: [Histonet] Freezing Haematoxylin Louise: You just stepped into "uncharted waters" because except for now when there seems to be some hematoxylin shortage, I think that what you ask was never tried before. So, as a present day Magellan, why don't you do the experiment and pass to all of us the results? Ren? J. --- On Fri, 8/15/08, louise renton wrote: From: louise renton Subject: [Histonet] Freezing Haematoxylin To: Histonet@lists.utsouthwestern.edu Date: Friday, August 15, 2008, 3:41 AM Dear all, Here's a question to tickle your brain cells before the weekend Can I freeze aliquots of Mayer's Haem (home made) for later use? Would there be any negative effects on composition/staining etc??? -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. From aschultz <@t> CMC-NH.ORG Fri Aug 15 12:50:08 2008 From: aschultz <@t> CMC-NH.ORG (Schultz, Amy) Date: Fri Aug 15 12:50:15 2008 Subject: [Histonet] New Pathology Lab in N.H.; Supervisor Opening Message-ID: <73A7ED895EE0C24D9267ED814911DF1907C22A08@exchange.cmc-nh.org> Technical Supervisor - Pathology, FT Days: Start Up a new Histology and Cytology Laboratory; Design Space, Purchase Equipment, Hire Staff and work on Implementation team for pathology computer module. Responsible for the supervision of the technical, operational and financial operations of the Histology/Cytology section including staff selection, orientation, training and daily direction of staff; is an active participant on the Laboratory Management Team. Bachelor Degree, ASCP certification or equivalent with 4 years clinical laboratory experience required. Amazing is just one of the adjectives used to describe our culture here at Catholic Medical Center. Here, you will work in a growing hospital laboratory with a world class staff, the latest technology, and state-of-the-art facilities. We provide an atmosphere that supports, challenges, and encourages our employees - that's hard to find today. This amazing culture is the reason for our astounding low turnover rate. Call today to find out more about us. catholicmedicalcenter .org Amy Schultz Laboratory Manager Catholic Medical Center 100 McGregor Street Manchester, NH 03102 603-663-6228 aschultz@cmc-nh.org The information contained in this email transmission is personal and confidential. It is intended for the use of the individual or entity to which it is addressed. If you are not the intended recipient, this electronic transmission must be deleted and notification made to the sender. Any unauthorized duplication, distribution or disclosure is prohibited. From Tbarnhart <@t> primecare.org Fri Aug 15 12:54:21 2008 From: Tbarnhart <@t> primecare.org (Barnhart, Tammy) Date: Fri Aug 15 12:53:48 2008 Subject: [Histonet] Freezing Haematoxylin Message-ID: <4F0B7161A6CD524FAD8017D52E1553407AE7BD@exchangent> Yep, if it freezes it's worthless. From a North Dakota histotech. We HAVE to make our own. Winter shipments of premaid hematoxylin always get too cold and the staining is very erratic. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Friday, August 15, 2008 12:08 PM To: louise renton; Histonet@lists.utsouthwestern.edu; Weems, Joyce Subject: RE: [Histonet] Freezing Haematoxylin Ah, the things you learn when living in the "cold area" of the country! Ren? J. --- On Fri, 8/15/08, Weems, Joyce wrote: From: Weems, Joyce Subject: RE: [Histonet] Freezing Haematoxylin To: "louise renton" , Histonet@lists.utsouthwestern.edu Date: Friday, August 15, 2008, 12:07 PM I don't think so... If hematoxylin freezes in shipping, it crystallizes and must be replaced. My 2 cents worth... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: Friday, August 15, 2008 3:41 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Freezing Haematoxylin Dear all, Here's a question to tickle your brain cells before the weekend Can I freeze aliquots of Mayer's Haem (home made) for later use? Would there be any negative effects on composition/staining etc??? -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From S.Mason <@t> open.ac.uk Fri Aug 15 16:19:35 2008 From: S.Mason <@t> open.ac.uk (S.Mason) Date: Fri Aug 15 16:19:47 2008 Subject: [Histonet] unidentified object Message-ID: Hi folks, Could anyone help me identify this object? It was found just outside the epineurium in rat sciatic nerve and is surrounded by what I presume to be RBCs. The staining is Passini's trichrome. I have ruled out sutures, so does anyone have any ideas? I would be most grateful to hear from you on s.mason@open.ac.uk if you have. I am away for a week so I apologise in advance if I don't reply immediately. Thank you for your interest. Kind regards, Sarah Mason PhD Student Life Sciences The Open University United Kingdom --------------------------------- The Open University is incorporated by Royal Charter (RC 000391), an exempt charity in England & Wales and a charity registered in Scotland (SC 038302). From S.Mason <@t> open.ac.uk Fri Aug 15 16:24:23 2008 From: S.Mason <@t> open.ac.uk (S.Mason) Date: Fri Aug 15 16:25:41 2008 Subject: [Histonet] FW: unidentified object References: Message-ID: Many apologies, I should have posted the picture to the histonet website. I have now sent it there under the filename of UHO. Thanks, and sorry once more. Sarah ________________________________ From: S.Mason Sent: Fri 15/08/2008 22:19 To: histonet@lists.utsouthwestern.edu Subject: unidentified object Hi folks, Could anyone help me identify this object? It was found just outside the epineurium in rat sciatic nerve and is surrounded by what I presume to be RBCs. The staining is Passini's trichrome. I have ruled out sutures, so does anyone have any ideas? I would be most grateful to hear from you on s.mason@open.ac.uk if you have. I am away for a week so I apologise in advance if I don't reply immediately. Thank you for your interest. Kind regards, Sarah Mason PhD Student Life Sciences The Open University United Kingdom --------------------------------- The Open University is incorporated by Royal Charter (RC 000391), an exempt charity in England & Wales and a charity registered in Scotland (SC 038302). From jnocito <@t> satx.rr.com Fri Aug 15 17:58:36 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Aug 15 17:57:40 2008 Subject: [Histonet] Documenting control tissue source? References: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F5AD@lmhsmail.lmhealth.org> Message-ID: <2A3B94B57B14440C823DA7624A136A12@yourxhtr8hvc4p> Tom, CAP used to require a log of all positive control tissues. We have a book that lists the date, accession #, stain/immuno used, the pathologist and the reactivity. I just reviewed the updated CAP checklist (9/27/07) and did not see that question listed. I don't know if it is an oversight that it was omitted or if CAP deleted the question. Joe ----- Original Message ----- From: "Tom McNemar" To: Sent: Friday, August 15, 2008 6:12 AM Subject: [Histonet] Documenting control tissue source? We are finishing up our JCAHO inspection today. The inspector asked about our control tissues and where we documented the source. We get nearly all of our controls from patient samples and she said that most histo labs mark their control blocks with the source case number. They then keep records of the control source used when staining new cases. I can see no logical reason to keep this info. You use a control appropriate for the element being stained. Why does it matter where the control came from? Am I wrong? Do you do this? Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Fri Aug 15 18:31:38 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Aug 15 18:30:37 2008 Subject: [Histonet] CLIA Regulation for the Histology laboratory. References: <8CACD0D8C4EE2D8-7D4-1CF0@WEBMAIL-MB01.sysops.aol.com> Message-ID: <98D9E116CC0643A594C9D45925FBFD87@yourxhtr8hvc4p> Jennell, here is the website for CLIA. Typical government fashion, never saw a website with wwwn. Joe http://wwwn.cdc.gov/clia/ ----- Original Message ----- From: To: Sent: Friday, August 15, 2008 11:46 AM Subject: [Histonet] CLIA Regulation for the Histology laboratory. > Hello histoneers, > > I'm trying to find the exact regulations governed by CLIA specifically for > histology. I was trying to find a website, but keep being redirected. If > anyone could send me some information that would be helpful. Thanks. > Jennell > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Mandy.Bell <@t> chomp.org Sat Aug 16 11:40:49 2008 From: Mandy.Bell <@t> chomp.org (Bell, Mandy) Date: Sat Aug 16 11:43:32 2008 Subject: [Histonet] jones basement membrane stain Message-ID: Hello. I'm looking for any help on the Jones stain for basement membranes. We're having trouble getting the basement membranes to stain dark enough, if at all. Here is our protocol (we are using a kit from American MasterTech): 0.5% Periodic Acid 11min rinse 2 changes distilled water methenamine silver (in a waterbath @ 65-70 degrees) ****we have tried varying the time by 15 minute increments ranging from 1 hour to 2 hours, 15min (we're checking the slides microscopically every ten minutes once the section is brown) rinse in distilled water 0.2% gold chloride 1 min rinse in distilled water 3% sodium thiosulfate 2 min rinse in distilled water nuclear fast red 4 min rinse dehydrate and clear Any help/ insight would be much appreciated! Thanks ! Mandy M Bell Histology Department Community Hospital of the Monterey Peninsula 831.625.4791 P Please consider the environment before printing this e-mail From rjbuesa <@t> yahoo.com Sat Aug 16 11:47:31 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Aug 16 11:47:34 2008 Subject: [Histonet] jones basement membrane stain In-Reply-To: Message-ID: <448993.41792.qm@web65712.mail.ac4.yahoo.com> It could be kit and the condition it is in. I for one never used kits, always prepared solutions myself. This could be the solution to your problems. Ren? J. --- On Sat, 8/16/08, Bell, Mandy wrote: From: Bell, Mandy Subject: [Histonet] jones basement membrane stain To: Histonet@lists.utsouthwestern.edu Date: Saturday, August 16, 2008, 12:40 PM Hello. I'm looking for any help on the Jones stain for basement membranes. We're having trouble getting the basement membranes to stain dark enough, if at all. Here is our protocol (we are using a kit from American MasterTech): 0.5% Periodic Acid 11min rinse 2 changes distilled water methenamine silver (in a waterbath @ 65-70 degrees) ****we have tried varying the time by 15 minute increments ranging from 1 hour to 2 hours, 15min (we're checking the slides microscopically every ten minutes once the section is brown) rinse in distilled water 0.2% gold chloride 1 min rinse in distilled water 3% sodium thiosulfate 2 min rinse in distilled water nuclear fast red 4 min rinse dehydrate and clear Any help/ insight would be much appreciated! Thanks ! Mandy M Bell Histology Department Community Hospital of the Monterey Peninsula 831.625.4791 P Please consider the environment before printing this e-mail _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Sun Aug 17 11:15:30 2008 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sun Aug 17 11:16:01 2008 Subject: AW: [Histonet] jones basement membrane stain In-Reply-To: Message-ID: <4184EE48CFC64BD48B3FBF7E9F97A0E9@dielangs.at> Hi Bell, You can try to prolong the incubation time untill the coplin jar turns black. Also the slides will get a shade of silver. With our protocol this is usually about 60-75 min in 60?C oven. (Best practice is to catch the moment just before the blackening of the glass begins) If this "breaking" of the silver-solution doesn't occur, I would think, that it is ineffective. Then don't wash the slides too excessive in water. Cleaning the slides back with paper towel is helpful. Goldchlorid turns the brown silver into black. Our protocol says 10 min in 0,1% Goldchloride. Hope this helps Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Bell, Mandy Gesendet: Samstag, 16. August 2008 18:41 An: Histonet@lists.utsouthwestern.edu Betreff: [Histonet] jones basement membrane stain Hello. I'm looking for any help on the Jones stain for basement membranes. We're having trouble getting the basement membranes to stain dark enough, if at all. Here is our protocol (we are using a kit from American MasterTech): 0.5% Periodic Acid 11min rinse 2 changes distilled water methenamine silver (in a waterbath @ 65-70 degrees) ****we have tried varying the time by 15 minute increments ranging from 1 hour to 2 hours, 15min (we're checking the slides microscopically every ten minutes once the section is brown) rinse in distilled water 0.2% gold chloride 1 min rinse in distilled water 3% sodium thiosulfate 2 min rinse in distilled water nuclear fast red 4 min rinse dehydrate and clear Any help/ insight would be much appreciated! Thanks ! Mandy M Bell Histology Department Community Hospital of the Monterey Peninsula 831.625.4791 P Please consider the environment before printing this e-mail _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Histowa13 <@t> aol.com Sun Aug 17 11:32:54 2008 From: Histowa13 <@t> aol.com (Histowa13@aol.com) Date: Sun Aug 17 11:33:02 2008 Subject: [Histonet] coverslippers Message-ID: If you live in an area where the humidity is high, tape is OK. If your climate tends to be drier, I would go with a glass coverslipper. Our experience has been that the tape pulls away from the slide taking the tissue with it. At that point it is almost impossible to save your slide. Debbie Nannenga, HTL, QIHC InCyte Pathology Spokane Valley, WA


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(http://autos.aol.com/cars-Volkswagen-Jetta-2009/expert-review?ncid=aolaut00030000000007 ) From aakrasht <@t> yahoo.com Sun Aug 17 12:54:13 2008 From: aakrasht <@t> yahoo.com (Ali A. Krasht) Date: Sun Aug 17 12:54:18 2008 Subject: [Histonet] PCR For NAILS Message-ID: <255203.39187.qm@web50907.mail.re2.yahoo.com> Hi There Anyone knows how to do PCR for nails (Histology) and what is the proceedure! Thanks for your help. Ali From llewllew <@t> shaw.ca Sun Aug 17 13:48:12 2008 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Sun Aug 17 13:48:34 2008 Subject: [Histonet] jones basement membrane stain References: <4184EE48CFC64BD48B3FBF7E9F97A0E9@dielangs.at> Message-ID: <000c01c90099$cd7716e0$0a514246@yourlk4rlmsu> Make sure the methenamine silver solution is fresh and you have the correct amount of borax or buffer in it. If that is so, try applying 0.5% aqueous thiosemicarbazide for ten minutes or so after the wash following periodic acid. Then wash well with water, rinse with distilled water and place in the methenamine silver at 60C until done. This usually speeds up the impregnation and increases contrast at the same time due to lighter background staining. Don't let the name of the chemical be a red flag. It is a semicarbazide not a metallic azide, and it is not explosive nor inflammable. It is safe. This procedure is useful for Gomori's methenamine silver as well. Bryan Llewellyn ========================= Hello. I'm looking for any help on the Jones stain for basement membranes. We're having trouble getting the basement membranes to stain dark enough, if at all. Here is our protocol (we are using a kit from American MasterTech): 0.5% Periodic Acid 11min rinse 2 changes distilled water methenamine silver (in a waterbath @ 65-70 degrees) ****we have tried varying the time by 15 minute increments ranging from 1 hour to 2 hours, 15min (we're checking the slides microscopically every ten minutes once the section is brown) rinse in distilled water 0.2% gold chloride 1 min rinse in distilled water 3% sodium thiosulfate 2 min rinse in distilled water nuclear fast red 4 min rinse dehydrate and clear Any help/ insight would be much appreciated! Thanks ! Mandy M Bell Histology Department Community Hospital of the Monterey Peninsula 831.625.4791 P Please consider the environment before printing this e-mail _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anastasiapinkston <@t> hotmail.com Sun Aug 17 16:02:04 2008 From: anastasiapinkston <@t> hotmail.com (Anastasia Pinkston) Date: Sun Aug 17 16:02:07 2008 Subject: [Histonet] (no subject) Message-ID: Ms. Bell 0.2% Gold Chloride for 1 min removes the silver staining from the tissue. If you decrease your time to 30 seconds you'd get a crisp clean stain. 0.1% Gold Chloride for 1 min tones the sliver but you'll have back ground staining. Betreff: [Histonet] jones basement membrane stain Hello.I'm looking for any help on the Jones stain for basement membranes. We'rehaving trouble getting the basement membranes to stain dark enough, if atall. Here is our protocol (we are using a kit from American MasterTech): 0.5% Periodic Acid 11minrinse 2 changes distilled watermethenamine silver (in a waterbath @ 65-70 degrees)****we have tried varying the time by 15 minute increments ranging from 1hour to 2 hours, 15min(we're checking the slides microscopically every ten minutes once thesection is brown)rinse in distilled water0.2% gold chloride 1 minrinse in distilled water3% sodium thiosulfate 2 minrinse in distilled waternuclear fast red 4 minrinsedehydrate and clear Any help/ insight would be much appreciated! Thanks ! Mandy M Bell Histology Department Community Hospital of the Monterey Peninsula 831.625.4791 _________________________________________________________________ Get thousands of games on your PC, your mobile phone, and the web with Windows?. http://clk.atdmt.com/MRT/go/108588800/direct/01/ From lpwenk <@t> sbcglobal.net Sun Aug 17 17:50:01 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sun Aug 17 17:50:08 2008 Subject: [Histonet] jones basement membrane stain In-Reply-To: Message-ID: <000701c900bb$950c1090$0202a8c0@HPPav2> It sounds like maybe the kit is bad. Either the periodic acid isn't oxidizing the carbohydrates on the basement membrane to aldehydes, or the methenamine silver solution is bad, so the silver won't bind. Possibly there is something wrong with the way the tissue was fixed or processed - not likely, but weird things have happened before. Some thoughts. First, I'm assuming you are using kits for many of your stains. Try substituting solutions from other kits, to find out what your problem is. 1. Get the periodic acid from the PASH kit, and use it instead of your current periodic acid. Then use the methenamine silver solution from the PASM kit. If the kidney stains now, then the original periodic acid was bad. 2. IF #1 doesn't solve the problem, get the methenamine silver solution from the GMS (Grocott) kit. If it's like our procedures (we make them up from scratch), the GMS methenamine silver solution will have about half the silver nitrate in it as the PASM methenamin silver solution. So use the periodic acid from the PASM kit, and substitute the methenamine silver from the GMS kit. It might take longer than your usual time. But if it works, then the silver solution was bad in the PASM kit. 3. Alternative, stain the section with a reticulin kit. If that doesn't stain the reticulin and basement membrane, then your tissue is bad. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bell, Mandy Sent: Saturday, August 16, 2008 12:41 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] jones basement membrane stain Hello. I'm looking for any help on the Jones stain for basement membranes. We're having trouble getting the basement membranes to stain dark enough, if at all. Here is our protocol (we are using a kit from American MasterTech): 0.5% Periodic Acid 11min rinse 2 changes distilled water methenamine silver (in a waterbath @ 65-70 degrees) ****we have tried varying the time by 15 minute increments ranging from 1 hour to 2 hours, 15min (we're checking the slides microscopically every ten minutes once the section is brown) rinse in distilled water 0.2% gold chloride 1 min rinse in distilled water 3% sodium thiosulfate 2 min rinse in distilled water nuclear fast red 4 min rinse dehydrate and clear Any help/ insight would be much appreciated! Thanks ! Mandy M Bell Histology Department Community Hospital of the Monterey Peninsula 831.625.4791 P Please consider the environment before printing this e-mail _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ynwang <@t> u.washington.edu Sun Aug 17 23:04:52 2008 From: ynwang <@t> u.washington.edu (Yak-Nam Wang) Date: Sun Aug 17 23:04:56 2008 Subject: [Histonet] get back eosin stain? Message-ID: Hello, I have a colleague with a staining problem. He has cells grown on polymeric scaffolds. He can not embedd in paraffin due to a mismatch in the processing and his scaffold material; he has thus embedded in OCT. This is his procedure: Fix cell seeded scaffold in formalin (these are delicate samples thus, fixing before embedding) Cryoprotect in grade sucrose to 30% sucrose Embed in OCT Section at 6um General H&E staining-using 1 min for Hematox and 1.5 min for Eosin (following one of Richard Allan suggested protocols). Problem: there is no eosin staining. The cells are just purple, there is no pink at all. We are not sure why this is. Is this caused by fixation? Does someone have any suggestions on how he can get the classic H&E stain look for these samples? He has tried fresh solutions as well as running other tissue (unfixed) through. It doesn't seem to be the solutions used. Any suggestions would be appreciated. Thank you Yak-Nam Reserach Associate CIMU University of Washington Box 355640 Seattle, WA 98195 Tel.: From marktarango <@t> gmail.com Mon Aug 18 00:25:12 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Mon Aug 18 00:25:19 2008 Subject: [Histonet] get back eosin stain? In-Reply-To: References: Message-ID: <5b6eb13e0808172225x6303538am97a147777e2f2026@mail.gmail.com> Hi Yak-Nam, The loss of eosinophilia is probably because the cells were cultured. I'd suggest trying a PAS and it might look more like what you'd like. Mark On 8/17/08, Yak-Nam Wang wrote: > Hello, > > I have a colleague with a staining problem. He has cells grown on polymeric > scaffolds. He can not embedd in paraffin due to a mismatch in the processing > and his scaffold material; he has thus embedded in OCT. > > This is his procedure: > Fix cell seeded scaffold in formalin (these are delicate samples thus, fixing before embedding) > Cryoprotect in grade sucrose to 30% sucrose > Embed in OCT > Section at 6um > General H&E staining-using 1 min for Hematox and 1.5 min for Eosin (following > one of Richard Allan suggested protocols). > > Problem: there is no eosin staining. The cells are just purple, there is no pink at all. We are not sure why this is. Is this caused by fixation? > > Does someone have any suggestions on how he can get the classic H&E stain look for these samples? He has tried fresh solutions as well as running other tissue (unfixed) through. It doesn't seem to be the solutions used. > > Any suggestions would be appreciated. Thank you > Yak-Nam > > > Reserach Associate > CIMU > University of Washington > Box 355640 > Seattle, WA 98195 > > Tel.: > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JHAPPEL <@t> PARTNERS.ORG Mon Aug 18 06:20:28 2008 From: JHAPPEL <@t> PARTNERS.ORG (Happel, James F.) Date: Mon Aug 18 06:20:43 2008 Subject: [Histonet] Job Opening in Boston MA References: Message-ID: Good Monday Morning Histonetters! I have an opening for a FT histologist for a busy Boston Massachusetts histology/pathology lab (predominately dermatology). Looking for a 1st shift (2:00 - 10:30 a.m.) Monday - Friday. If you know anyone interested please contact me at this E-mail or jhappel@pathsrv.com. Thanks and have a great Monday! James The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From Gguerzon <@t> lifebridgehealth.org Mon Aug 18 09:17:20 2008 From: Gguerzon <@t> lifebridgehealth.org (Godfrey Guerzon) Date: Mon Aug 18 09:17:34 2008 Subject: [Histonet] Procedures Message-ID: <48A94C30.704A.0068.0@lifebridgehealth.org> Anyone out in Histo Land willing to share their NCCLS format procedures for operating the Ventana XT and VIAS instruments? I would appreciate any help. Thanks. Godfrey ----------------------------------------------------------------- THE INFORMATION CONTAINED IN THIS MESSAGE IS LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION INTENDED FOR THE USE OF THE ADDRESSEE LISTED ABOVE. This record has been disclosed in accordance with Subtitle 3 of Title 4 of the Health-General Article of the Annotated Code of Maryland. Further disclosure of medical information contained herein is prohibited. If you are neither the intended recipient nor the individual responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure of patient information is strictly prohibited. If you have received this email in error, immediately notify us by telephone or return email. ----------------------------------------------------------------- From algranth <@t> u.arizona.edu Mon Aug 18 09:22:14 2008 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Mon Aug 18 09:22:19 2008 Subject: [Histonet] get back eosin stain? In-Reply-To: References: Message-ID: <6.2.3.4.1.20080818072016.026c5eb0@algranth.inbox.email.arizona.edu> > I see this a lot with cultured cells - even when processed and embedded in paraffin. Andi Grantham >Hello, > >I have a colleague with a staining problem. He has cells grown on polymeric >scaffolds. He can not embedd in paraffin due to a mismatch in the processing >and his scaffold material; he has thus embedded in OCT. > >This is his procedure: >Fix cell seeded scaffold in formalin (these are delicate samples >thus, fixing before embedding) >Cryoprotect in grade sucrose to 30% sucrose >Embed in OCT >Section at 6um >General H&E staining-using 1 min for Hematox and 1.5 min for Eosin (following >one of Richard Allan suggested protocols). > >Problem: there is no eosin staining. The cells are just purple, >there is no pink at all. We are not sure why this is. Is this caused >by fixation? > >Does someone have any suggestions on how he can get the classic H&E >stain look for these samples? He has tried fresh solutions as well >as running other tissue (unfixed) through. It doesn't seem to be the >solutions used. > >Any suggestions would be appreciated. Thank you >Yak-Nam > > >Reserach Associate >CIMU >University of Washington >Box 355640 >Seattle, WA 98195 > >Tel.: > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From Sandra.Harrison3 <@t> va.gov Mon Aug 18 09:46:38 2008 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Mon Aug 18 09:46:45 2008 Subject: [Histonet] microwave ovens recommendations, please Message-ID: OSHA section 29 CFR 1910.303 (b)(2) states that no food-grade microwave should be used for anything other than its intended purpose, namely, heating food for human consumption. CAP ANP.27170 "Are microwave devices used in accordance with manufacturer's instructions?" Due to the above CAP and OSHA regulations, I recently purchased a "lab-grade" microwave. I have experience the following problems: 1. floor heats up and is hot to the touch. 2. uneven staining of slides in racks. 3. variable temperatures, when heating up solutions used in microwave special stains. The manufacturer has not been able to satisfy my concerns, although they have tried by sending me a new floor that doesn't heat up and a carousel. Could I hear from other Histonetters what brand microwave oven they have and if they find it satisfactory? I don't need it for tissue processing, just slide drying and special stains. Thanks, Sandy From Janet.Bonner <@t> FLHOSP.ORG Mon Aug 18 09:46:18 2008 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Mon Aug 18 09:48:52 2008 Subject: [Histonet] coverslippers References: Message-ID: <5F31F38C96781A4FBE3196EBC22D47807F2786@fhosxchmb006.ADVENTISTCORP.NET> Florida Tech begs to differ! We had a horrible time with the slides where we used tape coverslipping. About five years after they were made the tissue lifted with the tape. We are now using the glass coverslippers. Janet L. Bonner, HTL (ASCP) Pathology Laboratory Florida Hospital Winter Park janet.bonner@FLHOSP.org 407-646-7559 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Histowa13@aol.com Sent: Sun 8/17/2008 12:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] coverslippers If you live in an area where the humidity is high, tape is OK. If your climate tends to be drier, I would go with a glass coverslipper. Our experience has been that the tape pulls away from the slide taking the tissue with it. At that point it is almost impossible to save your slide. Debbie Nannenga, HTL, QIHC InCyte Pathology Spokane Valley, WA


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If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From ynwang <@t> u.washington.edu Mon Aug 18 10:33:12 2008 From: ynwang <@t> u.washington.edu (Yak-Nam Wang) Date: Mon Aug 18 10:33:38 2008 Subject: [Histonet] get back eosin stain? In-Reply-To: <6.2.3.4.1.20080818072016.026c5eb0@algranth.inbox.email.arizona.edu> Message-ID: Do you know why this is? In the past, when I have cultured cells and frozen without fixation the cytoplasm stains pink. Is the lack of pink in these cells due to the fixation? Do you know if there is a way of fixing this (no pun intended!). Thank you Yak-Nam On 8/18/08 7:22 AM, "Andrea Grantham" wrote: > >> > > I see this a lot with cultured cells - even when processed and > embedded in paraffin. > > Andi Grantham > > >> Hello, >> >> I have a colleague with a staining problem. He has cells grown on polymeric >> scaffolds. He can not embedd in paraffin due to a mismatch in the processing >> and his scaffold material; he has thus embedded in OCT. >> >> This is his procedure: >> Fix cell seeded scaffold in formalin (these are delicate samples >> thus, fixing before embedding) >> Cryoprotect in grade sucrose to 30% sucrose >> Embed in OCT >> Section at 6um >> General H&E staining-using 1 min for Hematox and 1.5 min for Eosin (following >> one of Richard Allan suggested protocols). >> >> Problem: there is no eosin staining. The cells are just purple, >> there is no pink at all. We are not sure why this is. Is this caused >> by fixation? >> >> Does someone have any suggestions on how he can get the classic H&E >> stain look for these samples? He has tried fresh solutions as well >> as running other tissue (unfixed) through. It doesn't seem to be the >> solutions used. >> >> Any suggestions would be appreciated. Thank you >> Yak-Nam >> >> >> Reserach Associate >> CIMU >> University of Washington >> Box 355640 >> Seattle, WA 98195 >> >> Tel.: >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ..................................................................... > : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : > : Sr. Research Specialist University of Arizona : > : (office: AHSC 4212) P.O. Box 245044 : > : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : > : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : > :...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aysep <@t> yahoo.com Mon Aug 18 10:55:38 2008 From: aysep <@t> yahoo.com (ayse polat) Date: Mon Aug 18 10:55:42 2008 Subject: [Histonet] (no subject) Message-ID: <164164.36191.qm@web33708.mail.mud.yahoo.com> unsubscribe me, please From carrolpb <@t> umdnj.edu Mon Aug 18 11:03:30 2008 From: carrolpb <@t> umdnj.edu (Peter Carroll) Date: Mon Aug 18 11:03:53 2008 Subject: [Histonet] (no subject) In-Reply-To: <164164.36191.qm@web33708.mail.mud.yahoo.com> References: <164164.36191.qm@web33708.mail.mud.yahoo.com> Message-ID: <48A99D52.8090500@umdnj.edu> Did you see the link thats at the bottom of every mail you receive from this list? You can click it to unsubscribe yourself. this---> http://lists.utsouthwestern.edu/mailman/listinfo/histonet ayse polat wrote: > unsubscribe me, please > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From Marissa <@t> labcareer.com Mon Aug 18 11:05:15 2008 From: Marissa <@t> labcareer.com (Marissa Perez) Date: Mon Aug 18 11:05:22 2008 Subject: [Histonet] (no subject) In-Reply-To: <164164.36191.qm@web33708.mail.mud.yahoo.com> References: <164164.36191.qm@web33708.mail.mud.yahoo.com> Message-ID: <26A7728EF768DA478F45954252293C20F5E5CE@matrix.HCCI.local> Unsubscribe me too please! Best regards, Marissa Perez HealthCare Connections, Inc. Toll Free: (866) 346-8522 x 319 Fax: (954) 755-0819 E-mail: marissa@labcareer.com Website: www.labcareer.com If you would like to be removed from this mailing list, please click HERE -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ayse polat Sent: Monday, August 18, 2008 11:56 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) unsubscribe me, please _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Margaret.Perry <@t> sdstate.edu Mon Aug 18 11:06:18 2008 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Mon Aug 18 11:06:24 2008 Subject: [Histonet] Testing for IgA Message-ID: We have a researcher who is trying to do a procedure in the article "Alternate Mucosal Immune System: Organized Peyer's Patches Are Not Required for IgA Responses in the Gastrointestinal Tract" by Masafumi Yamamoto, Paul Rennert, Jerry R. McGhee, Mi-Na Kweon, Shingo Yamamoto, Taeko Dohi, Shiegeo Otake, Horst Bluethmann, Kohtaro Fujihashi and Hiroshi Kiyono. The article states "For IHC study, the jejunum and ileum were obtained from mice in each group for staining of Ab-containing cells. Briefly the tissues were fixed in 5% glacial acetic acid in 95% ethanol at -20 degrees C for 24 hours before paraffin embedding. Serial section 5?m thick were mounted on glass slides and IgA cells were visualized with FITC-labeled anti-mouse IgA mAb" Is there anyone who has done a procedure like this? Why are the sections put into paraffin and what type of instrument would be used to cut these? Would the tissues be processed and then embedded or would this destroy the IgA? Any help you can give us will be very much appreciated. Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 From SHargrove <@t> urhcs.org Mon Aug 18 11:37:29 2008 From: SHargrove <@t> urhcs.org (SHargrove@urhcs.org) Date: Mon Aug 18 11:37:55 2008 Subject: [Histonet] jones basement membrane stain (Gudrun Lang) In-Reply-To: Message-ID: Mandy, Have you tried the modified GMS? Similar to Jones . You just have the borax in the methenamine silver. Works well for us. Susie Hargrove H.T. Tech Specialist Histology United Regional 1600 11th Street Wichita Falls, Texas 76301 940-764-3881 From b-frederick <@t> northwestern.edu Mon Aug 18 11:41:54 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Aug 18 11:42:04 2008 Subject: [Histonet] Disaster plan for block and slide rescue Message-ID: <000001c90151$52ce7290$d00f7ca5@lurie.northwestern.edu> Hello all, This may be more applicable to tissue banks and research, particularly human, but does anyone have a disaster plan including rescue or salvage of blocks and/or slides? Has it ever crossed anyone's mind? Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 From amartinez <@t> carisdx.com Mon Aug 18 13:20:46 2008 From: amartinez <@t> carisdx.com (Martinez, Angela) Date: Mon Aug 18 13:20:50 2008 Subject: [Histonet] Job Opening In Ft. Worth Texas Message-ID: <9B8A3AC772C7F64680392A7CB8FBFB0F0570533A@s-irv-ex301.PathologyPartners.intranet> Great opportunity for Histotechnician in brand new laboratory! Gastrointestinal Associates of North Texas in Ft Worth , is looking for a certified HT or HTL to run their newly constructed laboratory. Candidate must be ASCP certified and meet CLIA-88 regulations to perform gross dissection. Prior supervisory experience preferred. The candidate will be responsible for the following: Creation and maintenance of policies and procedures to CLIA standards, leading lab through CLIA inspection, maintenance and quality control for equipment, and routine histology duties. The position offers competitive salary, medical insurance, retirement plan, and vacation /sick leave. Interested applicants should e-mail resumes to Meredith Hale at mhale@carisdx.com . Angela Martinez Internal Recruiter Caris Diagnostics 8400 Esters Blvd. Suite 190 Irving, Texas 75063 (214) 277-8700 Phone (214) 596-7490Fax From MadaryJ <@t> MedImmune.com Mon Aug 18 13:48:08 2008 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Mon Aug 18 13:48:31 2008 Subject: [Histonet] jones kidney(PAMS) Message-ID: I guess it is understood the sections are being cut at 2 microns? Nick Madary, HT/HTL(ASCP)QIHC Histology Mgr, Medimmune 301.398.6360(lab), 4745(vm),9745(fax) "To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary, and expected to be used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation." To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From dusko.trajkovic <@t> pfizer.com Mon Aug 18 14:59:27 2008 From: dusko.trajkovic <@t> pfizer.com (Trajkovic, Dusko) Date: Mon Aug 18 15:00:25 2008 Subject: [Histonet] Job Opening In Orlando Fl. In-Reply-To: <9B8A3AC772C7F64680392A7CB8FBFB0F0570533A@s-irv-ex301.PathologyPartners.intranet> Message-ID: <3AD0BD3142459B4E9B12CBEAFF2B89B207E26FF4@lajamrexm01.amer.pfizer.com> For additional information please contact me. Thanks Dusko Trajkovic Job Opening: Histology Technlogist Orlando, FL Date: August 2008 Job Title: Histology Technologist Facility/Department: Histology Core-Research Support Position Summary This position requires an individual with experience working in a histology core. The individual must have demonstrated ability to cooperate and work with others as demanded by the needs of a major research institute. Must be able to communicate effectively, demonstrate an interest in professional development, possess ability to define and resolve problems, and be able to work independently. The major responsibilities in this position will be to perform routine histology procedures, maintenance of equipment, reagents, and record keeping and billing of services. Critical Skills/Expertise Qualified candidate must demonstrate ability to work independently, cooperate and work with others, communicate effectively, have the ability to define and resolve problems, make technical decisions, and demonstrate an interest in professional development. Individual must provide technical expertise to ensure the smooth operation of the Histology core and the reliability of laboratory results for relevant research use. Qualified candidates must be able to accurate label and file slides and blocks, perform routine technical histology procedures including: accessioning specimens, embedding of tissue samples, specimen processing; sectioning of paraffin or frozen sections, antigen retrieval of paraffin embedded tissues, preparation of cytospin samples, and perform simple and special stains for immunohistochemistry or immunofluorescence. Individual must have the ability to maintain complete and accurate records, data reports, and be responsible for billing services performed by the histology core. Individual must operate and maintain complete documentation on all immunohistochemistry equipment, keep an adequate inventory of supplies, reagents and solutions, and dispose following proper regulations of any biohazard and potentially hazardous materials used in the histology core. Individual must participate in research for the use of new antibodies, verify batches of antibodies as needed, and troubleshoot problems with staining techniques and procedures. Education and Experience Qualified Candidate must have an Associates Degree or Bachelors Degree in Medical Technology or other biological Science with knowledge of biology, chemistry, anatomy and physiology. 1 to 2 years experience in histopathology techniques (embedding, cutting and staining slides). HT (ASCP) preferred. Individual must possess excellent interpersonal skills and exceptional attention to detail and accuracy From NMargaryan <@t> childrensmemorial.org Mon Aug 18 15:56:49 2008 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Mon Aug 18 15:57:33 2008 Subject: [Histonet] LCM and emryo Message-ID: Dear friends, Please, reply and suggest all of you who even tried once. One of our researchers asked me to laser capture from the chick embryo, using Veritas Arcturus LCM system. Is there anyone who has any idea how to fix chick embryo (I don't know age), probably whole embryo, and then how thick to section. I also need protocol to fix section and then to stain. In summary, I would be asking you completely protocol from beginning to the end. Sorry, I am asking about very much. Thanks in advance, Naira From marktarango <@t> gmail.com Mon Aug 18 16:14:42 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Mon Aug 18 16:14:48 2008 Subject: [Histonet] get back eosin stain? In-Reply-To: References: <6.2.3.4.1.20080818072016.026c5eb0@algranth.inbox.email.arizona.edu> Message-ID: <5b6eb13e0808181414g61ccd34ex86f4da9559923222@mail.gmail.com> Were the cells that you cultured this time of the same type as before? Is it the same media? Were they cultured for just as long? I still say try the PAS, you originally asked for the "look" of an H&E. I did spit on one once and the stain came back a little. Sometimes I do strange things like that. Probably shouldn't have even mentioned it. Mark On Mon, Aug 18, 2008 at 8:33 AM, Yak-Nam Wang wrote: > Do you know why this is? In the past, when I have cultured cells and frozen > without fixation the cytoplasm stains pink. Is the lack of pink in these > cells due to the fixation? Do you know if there is a way of fixing this (no > pun intended!). > > Thank you > Yak-Nam > > > On 8/18/08 7:22 AM, "Andrea Grantham" wrote: > >> >>> >> >> I see this a lot with cultured cells - even when processed and >> embedded in paraffin. >> >> Andi Grantham >> >> >>> Hello, >>> >>> I have a colleague with a staining problem. He has cells grown on polymeric >>> scaffolds. He can not embedd in paraffin due to a mismatch in the processing >>> and his scaffold material; he has thus embedded in OCT. >>> >>> This is his procedure: >>> Fix cell seeded scaffold in formalin (these are delicate samples >>> thus, fixing before embedding) >>> Cryoprotect in grade sucrose to 30% sucrose >>> Embed in OCT >>> Section at 6um >>> General H&E staining-using 1 min for Hematox and 1.5 min for Eosin (following >>> one of Richard Allan suggested protocols). >>> >>> Problem: there is no eosin staining. The cells are just purple, >>> there is no pink at all. We are not sure why this is. Is this caused >>> by fixation? >>> >>> Does someone have any suggestions on how he can get the classic H&E >>> stain look for these samples? He has tried fresh solutions as well >>> as running other tissue (unfixed) through. It doesn't seem to be the >>> solutions used. >>> >>> Any suggestions would be appreciated. Thank you >>> Yak-Nam >>> >>> >>> Reserach Associate >>> CIMU >>> University of Washington >>> Box 355640 >>> Seattle, WA 98195 >>> >>> Tel.: >>> >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> ..................................................................... >> : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : >> : Sr. Research Specialist University of Arizona : >> : (office: AHSC 4212) P.O. Box 245044 : >> : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : >> : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : >> :...................................................................: >> http://www.cba.arizona.edu/histology-lab.html >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From llewllew <@t> shaw.ca Mon Aug 18 17:07:04 2008 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Mon Aug 18 17:07:30 2008 Subject: [Histonet] get back eosin stain? References: <6.2.3.4.1.20080818072016.026c5eb0@algranth.inbox.email.arizona.edu> <5b6eb13e0808181414g61ccd34ex86f4da9559923222@mail.gmail.com> Message-ID: <002601c9017e$bf7eb8f0$0a514246@yourlk4rlmsu> It is sometimes possible to stain tissues with eosinol when they do not stain with eosin Y. Eosinol is the free anion of eosin Y and is not soluble in water. You can sometimes buy it, or can make it from a strong aqueous solution of eosin Y by dropping in concentrated hydrochloric acid until maximum precipitate is obtained, then filtering and washing several times with distilled water to remove the acid. When dried, a small amount added to the final xylene (yes, xylene) will colour the tissue. When dark enough, coverslip. Bryan Llewellyn >>> >>>> Hello, >>>> >>>> I have a colleague with a staining problem. He has cells grown on >>>> polymeric >>>> scaffolds. He can not embedd in paraffin due to a mismatch in the >>>> processing >>>> and his scaffold material; he has thus embedded in OCT. >>>> >>>> This is his procedure: >>>> Fix cell seeded scaffold in formalin (these are delicate samples >>>> thus, fixing before embedding) >>>> Cryoprotect in grade sucrose to 30% sucrose >>>> Embed in OCT >>>> Section at 6um >>>> General H&E staining-using 1 min for Hematox and 1.5 min for Eosin >>>> (following >>>> one of Richard Allan suggested protocols). >>>> >>>> Problem: there is no eosin staining. The cells are just purple, >>>> there is no pink at all. We are not sure why this is. Is this caused >>>> by fixation? >>>> >>>> Does someone have any suggestions on how he can get the classic H&E >>>> stain look for these samples? He has tried fresh solutions as well >>>> as running other tissue (unfixed) through. It doesn't seem to be the >>>> solutions used. >>>> >>>> Any suggestions would be appreciated. Thank you >>>> Yak-Nam >>>> >>>> >>>> Reserach Associate >>>> CIMU >>>> University of Washington >>>> Box 355640 >>>> Seattle, WA 98195 >>>> >>>> Tel.: >>>> >>>> >>>> >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> ..................................................................... >>> : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : >>> : Sr. Research Specialist University of Arizona : >>> : (office: AHSC 4212) P.O. Box 245044 : >>> : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : >>> : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : >>> :...................................................................: >>> http://www.cba.arizona.edu/histology-lab.html >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lldewe <@t> gmail.com Mon Aug 18 19:18:54 2008 From: lldewe <@t> gmail.com (Loralei Dewe) Date: Mon Aug 18 19:19:00 2008 Subject: [Histonet] Mammary tissue specific stain Message-ID: <7173d3c00808181718l752cee79p1bbef07a50fc98c0@mail.gmail.com> Hi All, I am looking for stain that will stain mouse mammary gland tissue without picking up the surrounding background lipids. Any ideas? I could be either a special type stain or antibody stain?? Cheers, Lorie From raj <@t> bluemarble.net Mon Aug 18 19:47:29 2008 From: raj <@t> bluemarble.net (Rebecca Johnson) Date: Mon Aug 18 19:47:32 2008 Subject: [Histonet] Prisma coverslipper Message-ID: <001401c88a2c$25d74c40$7b48f9d8@CHURCH> Is anyone out there having trouble with the Prisma coverslipper on cytology image slides? As for histology we are having no problems. Thank You Becky From koellingr <@t> comcast.net Mon Aug 18 22:35:55 2008 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Mon Aug 18 22:36:04 2008 Subject: [Histonet] Mammary tissue specific stain Message-ID: <081920080335.21776.48AA3F9B0003303C0000551022070206539D09020704040A0105@comcast.net> Lorie, Not understanding exactly what is your project. With flat whole mounts of mouse mammary tissue, you can do a whole mount alum-carmine stain to stain the branching network of ducts and terminal sacs. It is in literature hundreds of times over. Essentially you are dissolving a lot of lipds so the branch work stands out in 3-D through the whole mount under a scope. And you can process that whole mount, make normal slides and still see the mammary glands and branches or even re-stain in carmine/mucicarmine to make staining stand out even more. Or do a cytokeratin stain on slides to see the breast tissue. Of course the slides will be 6 micron sections, so a thin portion, compared to whole mount carmine stains. Have done all of these things but it depends on what specifically you are after. Ray Koelling PhenoPath Labs Seattle, WA -------------- Original message -------------- From: "Loralei Dewe" > Hi All, > > I am looking for stain that will stain mouse mammary gland tissue without > picking up the surrounding background lipids. Any ideas? I could be either a > special type stain or antibody stain?? > > Cheers, > Lorie > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Tue Aug 19 05:36:19 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Aug 19 05:35:48 2008 Subject: [Histonet] Microwave safety Message-ID: <3369978680CE42B7BFAE58F0D9170A25@yourxhtr8hvc4p> Histoland, has anyone ever experienced or heard of an accident while using a "for home use only" microwave? I have never had an accident. Before I go on another tangent about CAP and OSHA, I'd like to know what everyone else experience is. Since everyone is running around, changing the way they do things just because CAP says breast cases have to be documented as to the length of time these cases have been fixing in formalin. Uh-oh, I feel it coming on. Another tangent. I feel the flames warming up. JTT From annigyg <@t> gmail.com Tue Aug 19 07:46:25 2008 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Tue Aug 19 07:46:30 2008 Subject: [Histonet] Microwave safety In-Reply-To: <3369978680CE42B7BFAE58F0D9170A25@yourxhtr8hvc4p> References: <3369978680CE42B7BFAE58F0D9170A25@yourxhtr8hvc4p> Message-ID: hey joe i have had accidents in my kitchen - but never in the lab im also getting a little tired of the CAP dictatorship - and we have not even started yet!!! if they were all dyed-in-the-wool histotechs with years and years of experience i would find it easier to accept - but then again if they had all that histo experience they would certainly not be expecting/demanding all these silly 'improvements' am also flipping annoyed that i am being told which EQA i can and cannot use - the list of CAP approved EQA facilities does not include NEQAS or RCPA QAP, both of which i use and am extremely satisfied with .... CAP are all about the money thats my vent for the decade whew....i feel better already!!!! abudhabiannie 2008/8/19 Joe Nocito > Histoland, > has anyone ever experienced or heard of an accident while using a "for home > use only" microwave? I have never had an accident. Before I go on another > tangent about CAP and OSHA, I'd like to know what everyone else experience > is. Since everyone is running around, changing the way they do things just > because CAP says breast cases have to be documented as to the length of time > these cases have been fixing in formalin. Uh-oh, I feel it coming on. > Another tangent. I feel the flames warming up. > > JTT > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From rjbuesa <@t> yahoo.com Tue Aug 19 07:57:04 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Aug 19 07:57:09 2008 Subject: [Histonet] Microwave safety In-Reply-To: Message-ID: <60879.7437.qm@web65715.mail.ac4.yahoo.com> Joe and Anne: Have you both forgotten that every time a lab buys a cheap?"home" microwave, the large lab equipment?manufacturing companies lose a sale? Have you forgotten that if an accident happens (no matter how remote the possibility is) any "good claims attorney" will be able to twist the issue to "nail" somebody for using an equipment "against the manufacturer's specifications"? We are talking about monetary interests, not about personnel safety! Ren? J. --- On Tue, 8/19/08, Anne van Binsbergen wrote: From: Anne van Binsbergen Subject: Re: [Histonet] Microwave safety To: "Joe Nocito" Cc: "histonet" Date: Tuesday, August 19, 2008, 8:46 AM hey joe i have had accidents in my kitchen - but never in the lab im also getting a little tired of the CAP dictatorship - and we have not even started yet!!! if they were all dyed-in-the-wool histotechs with years and years of experience i would find it easier to accept - but then again if they had all that histo experience they would certainly not be expecting/demanding all these silly 'improvements' am also flipping annoyed that i am being told which EQA i can and cannot use - the list of CAP approved EQA facilities does not include NEQAS or RCPA QAP, both of which i use and am extremely satisfied with .... CAP are all about the money thats my vent for the decade whew....i feel better already!!!! abudhabiannie 2008/8/19 Joe Nocito > Histoland, > has anyone ever experienced or heard of an accident while using a "for home > use only" microwave? I have never had an accident. Before I go on another > tangent about CAP and OSHA, I'd like to know what everyone else experience > is. Since everyone is running around, changing the way they do things just > because CAP says breast cases have to be documented as to the length of time > these cases have been fixing in formalin. Uh-oh, I feel it coming on. > Another tangent. I feel the flames warming up. > > JTT > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Tue Aug 19 09:39:07 2008 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Tue Aug 19 09:39:12 2008 Subject: [Histonet] Rabbit on rabbit IHC Message-ID: <63EA0607835FBA4689CEA9EA8B482692014BF310@usctmx1141.merck.com> Hi all, Looking for suggestions on performing IHC w/ rabbit monoclonal on rabbit tissue....or does anyone know of a mouse/rat monoclonal for Cleaved Caspase-3? Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Research Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From sotlak <@t> yahoo.gr Tue Aug 19 10:01:26 2008 From: sotlak <@t> yahoo.gr (sotiris lakis) Date: Tue Aug 19 10:01:32 2008 Subject: [Histonet] tissue container disposal Message-ID: <975537.88832.qm@web23004.mail.ird.yahoo.com> Hello everybody, I know disposal policies might differ between countries but we seem to be facing a dead end concerning used empty tissue containers with formaldehyde remnants. We are provided with a toxic waste container for liquids and special bags for biohazardous material. There seems to be some confusion among colleagues so I would appreciate it if someone could give us an idea about the best way to solve the problem. Thanks S. Lakis Resident in Pathology --------------------------------- ?????????????? Yahoo! ?????????? ?? ?????????? ???? ???? (spam); ?? Yahoo! Mail ???????? ??? ???????? ?????? ????????? ???? ??? ??????????? ????????? http://login.yahoo.com/config/mail?.intl=gr From JWeems <@t> sjha.org Tue Aug 19 10:08:07 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Aug 19 10:08:17 2008 Subject: [Histonet] Microwave safety In-Reply-To: <60879.7437.qm@web65715.mail.ac4.yahoo.com> References: <60879.7437.qm@web65715.mail.ac4.yahoo.com> Message-ID: <982A0A9461F9BF438C7B19A6E425A3832017B7@ITSSSXM01V6.one.ads.che.org> The sad thing is that we all know this, but it continues to be a growing issue in our country. What can we do to stop it? It's like a run-away train? j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, August 19, 2008 8:57 AM To: Joe Nocito; Anne van Binsbergen Cc: histonet Subject: Re: [Histonet] Microwave safety Joe and Anne: Have you both forgotten that every time a lab buys a cheap?"home" microwave, the large lab equipment?manufacturing companies lose a sale? Have you forgotten that if an accident happens (no matter how remote the possibility is) any "good claims attorney" will be able to twist the issue to "nail" somebody for using an equipment "against the manufacturer's specifications"? We are talking about monetary interests, not about personnel safety! Ren? J. --- On Tue, 8/19/08, Anne van Binsbergen wrote: From: Anne van Binsbergen Subject: Re: [Histonet] Microwave safety To: "Joe Nocito" Cc: "histonet" Date: Tuesday, August 19, 2008, 8:46 AM hey joe i have had accidents in my kitchen - but never in the lab im also getting a little tired of the CAP dictatorship - and we have not even started yet!!! if they were all dyed-in-the-wool histotechs with years and years of experience i would find it easier to accept - but then again if they had all that histo experience they would certainly not be expecting/demanding all these silly 'improvements' am also flipping annoyed that i am being told which EQA i can and cannot use - the list of CAP approved EQA facilities does not include NEQAS or RCPA QAP, both of which i use and am extremely satisfied with .... CAP are all about the money thats my vent for the decade whew....i feel better already!!!! abudhabiannie 2008/8/19 Joe Nocito > Histoland, > has anyone ever experienced or heard of an accident while using a "for home > use only" microwave? I have never had an accident. Before I go on another > tangent about CAP and OSHA, I'd like to know what everyone else experience > is. Since everyone is running around, changing the way they do things just > because CAP says breast cases have to be documented as to the length > of time > these cases have been fixing in formalin. Uh-oh, I feel it coming on. > Another tangent. I feel the flames warming up. > > JTT > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From rjbuesa <@t> yahoo.com Tue Aug 19 10:28:37 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Aug 19 10:28:47 2008 Subject: [Histonet] Microwave safety In-Reply-To: <982A0A9461F9BF438C7B19A6E425A3832017B7@ITSSSXM01V6.one.ads.che.org> Message-ID: <455370.95459.qm@web65701.mail.ac4.yahoo.com> Yes, the train left the station long time ago, precisely the moment the CAP embraced this issue and decided to enforce it! Ren? J. --- On Tue, 8/19/08, Weems, Joyce wrote: From: Weems, Joyce Subject: RE: [Histonet] Microwave safety To: rjbuesa@yahoo.com Cc: "histonet" Date: Tuesday, August 19, 2008, 11:08 AM The sad thing is that we all know this, but it continues to be a growing issue in our country. What can we do to stop it? It's like a run-away train? j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, August 19, 2008 8:57 AM To: Joe Nocito; Anne van Binsbergen Cc: histonet Subject: Re: [Histonet] Microwave safety Joe and Anne: Have you both forgotten that every time a lab buys a cheap?"home" microwave, the large lab equipment?manufacturing companies lose a sale? Have you forgotten that if an accident happens (no matter how remote the possibility is) any "good claims attorney" will be able to twist the issue to "nail" somebody for using an equipment "against the manufacturer's specifications"? We are talking about monetary interests, not about personnel safety! Ren? J. --- On Tue, 8/19/08, Anne van Binsbergen wrote: From: Anne van Binsbergen Subject: Re: [Histonet] Microwave safety To: "Joe Nocito" Cc: "histonet" Date: Tuesday, August 19, 2008, 8:46 AM hey joe i have had accidents in my kitchen - but never in the lab im also getting a little tired of the CAP dictatorship - and we have not even started yet!!! if they were all dyed-in-the-wool histotechs with years and years of experience i would find it easier to accept - but then again if they had all that histo experience they would certainly not be expecting/demanding all these silly 'improvements' am also flipping annoyed that i am being told which EQA i can and cannot use - the list of CAP approved EQA facilities does not include NEQAS or RCPA QAP, both of which i use and am extremely satisfied with .... CAP are all about the money thats my vent for the decade whew....i feel better already!!!! abudhabiannie 2008/8/19 Joe Nocito > Histoland, > has anyone ever experienced or heard of an accident while using a "for home > use only" microwave? I have never had an accident. Before I go on another > tangent about CAP and OSHA, I'd like to know what everyone else experience > is. Since everyone is running around, changing the way they do things just > because CAP says breast cases have to be documented as to the length > of time > these cases have been fixing in formalin. Uh-oh, I feel it coming on. > Another tangent. I feel the flames warming up. > > JTT > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From rjbuesa <@t> yahoo.com Tue Aug 19 10:32:06 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Aug 19 10:32:10 2008 Subject: [Histonet] tissue container disposal In-Reply-To: <975537.88832.qm@web23004.mail.ird.yahoo.com> Message-ID: <848056.11263.qm@web65703.mail.ac4.yahoo.com> The formalin in the used containers have to be emptied in the large container for formalin to be removed by a specialized contractor. The containers can be either incinerated (if plastic) or given to the same contractor for disposal. A used container cannot be disposed off with the formalin inside. There are some variations by state and hospital. Ren? J. --- On Tue, 8/19/08, sotiris lakis wrote: From: sotiris lakis Subject: [Histonet] tissue container disposal To: histonet@lists.utsouthwestern.edu Date: Tuesday, August 19, 2008, 11:01 AM Hello everybody, I know disposal policies might differ between countries but we seem to be facing a dead end concerning used empty tissue containers with formaldehyde remnants. We are provided with a toxic waste container for liquids and special bags for biohazardous material. There seems to be some confusion among colleagues so I would appreciate it if someone could give us an idea about the best way to solve the problem. Thanks S. Lakis Resident in Pathology --------------------------------- ?????????????? Yahoo! ?????????? ?? ?????????? ???? ???? (spam); ?? Yahoo! Mail ???????? ??? ???????? ?????? ????????? ???? ??? ??????????? ????????? http://login.yahoo.com/config/mail?.intl=gr _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Aug 19 10:32:58 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Aug 19 10:33:05 2008 Subject: [Histonet] tissue container disposal Message-ID: <802416.53857.qm@web65712.mail.ac4.yahoo.com> The formalin in the used containers have to be emptied in the large container for formalin to be removed by a specialized contractor. The containers can be either incinerated (if plastic) or given to the same contractor for disposal. A used container cannot be disposed off with the formalin inside. There are some variations by state and hospital. Ren? J. --- On Tue, 8/19/08, sotiris lakis wrote: From: sotiris lakis Subject: [Histonet] tissue container disposal To: histonet@lists.utsouthwestern.edu Date: Tuesday, August 19, 2008, 11:01 AM Hello everybody, I know disposal policies might differ between countries but we seem to be facing a dead end concerning used empty tissue containers with formaldehyde remnants. We are provided with a toxic waste container for liquids and special bags for biohazardous material. There seems to be some confusion among colleagues so I would appreciate it if someone could give us an idea about the best way to solve the problem. Thanks S. Lakis Resident in Pathology --------------------------------- ?????????????? Yahoo! ?????????? ?? ?????????? ???? ???? (spam); ?? Yahoo! Mail ???????? ??? ???????? ?????? ????????? ???? ??? ??????????? ????????? http://login.yahoo.com/config/mail?.intl=gr _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carlos <@t> clubstaffing.com Tue Aug 19 10:49:14 2008 From: carlos <@t> clubstaffing.com (Carlos Stolk) Date: Tue Aug 19 10:49:20 2008 Subject: [Histonet] tissue container disposal In-Reply-To: <802416.53857.qm@web65712.mail.ac4.yahoo.com> References: <802416.53857.qm@web65712.mail.ac4.yahoo.com> Message-ID: Are there are different rules and regulations that determine what is and what is not legal in any given state/hospital? Carlos Stolk Account Representative Georgia | New Mexico South Carolina| Texas Phone: 800-875-8999 ext. 258 Fax: 561-367-0884 carlos@clubstaffing.com Club Staffing, Inc. 5901 Broken Sound Pkwy, Ste 500 Boca Raton, FL 33487 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, August 19, 2008 11:33 AM To: histonet@lists.utsouthwestern.edu; sotiris lakis Subject: Re: [Histonet] tissue container disposal The formalin in the used containers have to be emptied in the large container for formalin to be removed by a specialized contractor. The containers can be either incinerated (if plastic) or given to the same contractor for disposal. A used container cannot be disposed off with the formalin inside. There are some variations by state and hospital. Ren? J. --- On Tue, 8/19/08, sotiris lakis wrote: From: sotiris lakis Subject: [Histonet] tissue container disposal To: histonet@lists.utsouthwestern.edu Date: Tuesday, August 19, 2008, 11:01 AM Hello everybody, I know disposal policies might differ between countries but we seem to be facing a dead end concerning used empty tissue containers with formaldehyde remnants. We are provided with a toxic waste container for liquids and special bags for biohazardous material. There seems to be some confusion among colleagues so I would appreciate it if someone could give us an idea about the best way to solve the problem. Thanks S. Lakis Resident in Pathology --------------------------------- ?????????????? Yahoo! ?????????? ?? ?????????? ???? ???? (spam); ?? Yahoo! Mail ???????? ??? ???????? ?????? ????????? ???? ??? ??????????? ????????? http://login.yahoo.com/config/mail?.intl=gr _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LINDA.MARGRAF <@t> childrens.com Tue Aug 19 11:00:44 2008 From: LINDA.MARGRAF <@t> childrens.com (LINDA MARGRAF) Date: Tue Aug 19 11:02:42 2008 Subject: [Histonet] job listing Message-ID: <48AAA7DB.F783.00DA.0@childrens.com> Here's a job Beth asked me to post. Please contact her with inquires. Thanks. Technical Applications specialist- laboratory ESSENTIAL DUTIES AND RESPONSIBILITIES ? Installation of instrumentation and conducting user training to successfully integrate products within the customer laboratory. ? Provide troubleshooting and minor repair to instrumentation. ? Work with customers to resolve reagent/staining application issues. ? Communicate with dispatch and area management on daily/weekly/monthly activities (eg. Salesforce.com) ? Maintaining tools and test equipment in accordance with Quality procedures ? Conduct in-service training for customers. ? Customer follow-upSubmit administrative paperwork in a timely and accurate manner. ? installation forms ? service work orders ? expense reports ? Travel extensively within the region and throughout the country. REQUIRED EDUCATION & EXPERIENCE Associates degree (or equivalent) or higher in Applied Sciences or accredited Medical/Histology Technology course. Minimum one year general clinical laboratory experience or technical equivalent which may include general histology, cytology, immunohistochemistry, in situ hybridization, biology research or work with automated staining instrumentation. CERTIFICATES, LICENSES, REGISTRATIONS Highly desirable: HISTOLOGY TECHNICIAN American Society of Clinical Pathologists Certification HT or HTL. American Society of Clinical Pathologists Qualification in Immunohistochemistry (QIHC). KNOWLEDGE, SKILLS & ABILITIES Must have the ability to read, analyze, and interpret technical procedures or governmental regulations (CAP, CLIA). Ability to write reports. Ability to effectively present information and respond to questions from customers. Ability to calculate antibody dilutions. Ability to interpret a variety of instructions furnished in written, verbal, diagram, or schedule form. WORK ENVIRONMENT The work environment characteristics described here are representative of those an employee encounters while performing the essential functions of this job traveling to and working within a Clinical Pathology Lab, Research lab, and animal diagnostic lab. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions. OTHER QUALIFICATIONS Ability to travel. Valid driver*s license. with a clean driving record. COMPENSATION PACKAGE POSITION OFFERS A COMPETITVE BASE SALARY AND A CAR PLUS ALL TRAVEL EXPENSES AND BENEFITS. CONTACT BETH TEWS RECRUITING BETHTEWS@HOTMAIL.COM 623-742-7227 OPENINGS CURRENTLY NEED TO BE FILLED BY 715/08 IN PHOENIX, Minneapolis, and HOUSTON MORE TO COME SO PLEASE APPLY Please consider the environment before printing this e-mail

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From kcai <@t> prosci-inc.com Tue Aug 19 11:24:30 2008 From: kcai <@t> prosci-inc.com (karen Cai) Date: Tue Aug 19 11:17:33 2008 Subject: [Histonet] Help with IF in Chamber slide Message-ID: <000a01c90218$0f58ac70$7d01a8c0@prosci.com> Hello, I need to do the IF the 8 well Lab-tek chamber slides. Does anybody have the experience of this? How to culture the cells before blocking? Is glass type good or using permanox? The cell I used is CHO cell. Membrane protein. Thanks in advance, Best, Karen No virus found in this outgoing message. Checked by AVG. Version: 7.5.524 / Virus Database: 270.6.5/1620 - Release Date: 8/19/2008 6:04 AM From Mandy.Bell <@t> chomp.org Tue Aug 19 11:18:17 2008 From: Mandy.Bell <@t> chomp.org (Bell, Mandy) Date: Tue Aug 19 11:18:33 2008 Subject: [Histonet] jones basement membrane stain Message-ID: Thanks everyone for your help. We are running a couple of variations based on all of your recommendations. Will let you know how it turns out! Thanks for all of the feedback. Mandy M Bell Histology Department Community Hospital of the Monterey Peninsula 831.625.4791 P Please consider the environment before printing this e-mail From nfournier <@t> sasktel.net Tue Aug 19 12:36:32 2008 From: nfournier <@t> sasktel.net (N Fournier) Date: Tue Aug 19 12:36:37 2008 Subject: [Histonet] in situ question Message-ID: Hi, I have a few questions regarding in situ hybridization. The protocol that I am following indicates that they perfused rats with PBS followed by 4% paraformaledhyde (in PBS). However, I always thought that one generally extracts the rat brain fresh, flash freeze, section, and then post-fix in 4% paraformaldehyde before commencing with the in situ hybridization. I suppose it might not matter since in the end the tissue is post-fixed in PARA; however, I would like to hear what others with experience doing in situ think about this procedure. Additionally, does one always use DEPC treated solutions even for these perfusion steps (e.g., PBS perfusion, paraformaldehyde perfusion)? Lastly, does one have to section on a cryostat? Could I simply use a vibrating microtome to section the tissue instead. Thanks for the help everyone, Neil From JWeems <@t> sjha.org Tue Aug 19 12:44:52 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Tue Aug 19 12:45:59 2008 Subject: [Histonet] Blue Telfa Pads Message-ID: <982A0A9461F9BF438C7B19A6E425A3832017BF@ITSSSXM01V6.one.ads.che.org> Does anyone have a source for sterile blue Telfa pads? Thanks in advance. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From Loralee_Mcmahon <@t> URMC.Rochester.edu Tue Aug 19 12:44:48 2008 From: Loralee_Mcmahon <@t> URMC.Rochester.edu (McMahon, Loralee A) Date: Tue Aug 19 12:48:54 2008 Subject: [Histonet] in situ question References: Message-ID: <2CF6F6B05263EA4EBAB07781B51E5DB0025A289D@e2k3ms1.urmc-sh.rochester.edu> We perform ISH on formalin fixed paraffin embedded human tissue. This tissues come from surgical pathology so they are just routinely removed and fixed. No special treatment. We cut the slides fresh using RNAse free everything. New knife, gloves, distilled water etc. Works very good. Loralee McMahon, HTL (ASCP) ICC Supervisor University of Rochester Department of Pathology (585) 275-7210 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of N Fournier Sent: Tue 8/19/2008 1:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] in situ question Hi, I have a few questions regarding in situ hybridization. The protocol that I am following indicates that they perfused rats with PBS followed by 4% paraformaledhyde (in PBS). However, I always thought that one generally extracts the rat brain fresh, flash freeze, section, and then post-fix in 4% paraformaldehyde before commencing with the in situ hybridization. I suppose it might not matter since in the end the tissue is post-fixed in PARA; however, I would like to hear what others with experience doing in situ think about this procedure. Additionally, does one always use DEPC treated solutions even for these perfusion steps (e.g., PBS perfusion, paraformaldehyde perfusion)? Lastly, does one have to section on a cryostat? Could I simply use a vibrating microtome to section the tissue instead. Thanks for the help everyone, Neil _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MMargiotta <@t> bmhmc.org Tue Aug 19 13:03:44 2008 From: MMargiotta <@t> bmhmc.org (Margiotta, Michele) Date: Tue Aug 19 13:04:38 2008 Subject: [Histonet] part-time opening Message-ID: Hi All, We currently have a part-time opening for a qualified histotech. Responsibilities would include embedding, cutting and equipment maintenance. The hospital is located in eastern Suffolk County on Long Island. Anyone interested, please call the number below. Thanks, Michele Margiotta BMHMC Histology Supervisor 631-654-7192 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. From lmoak <@t> hardmanpath.com Tue Aug 19 15:18:40 2008 From: lmoak <@t> hardmanpath.com (Moak, Linda C.) Date: Tue Aug 19 15:16:54 2008 Subject: [Histonet] HPV Immunos Message-ID: <9B93AC540CFAF243831056D476EAAB910DB669@cpexch01.cunninghampathology.com> Does anybody know of reagents specific for each HPV 1, 6, 11, 16, 18 and 31 on condyloma specimens and where the antibodies can be obtained? Thanks for any info you can share with us. Linda C. Moak-Office Manager Hardman Pathology ADX lmoak@hardmanpath.com From ynwang <@t> u.washington.edu Tue Aug 19 15:28:52 2008 From: ynwang <@t> u.washington.edu (Yak-Nam Wang) Date: Tue Aug 19 15:29:18 2008 Subject: [Histonet] get back eosin stain? In-Reply-To: <002601c9017e$bf7eb8f0$0a514246@yourlk4rlmsu> Message-ID: Thank you for all your suggestions. He tried extending the eoson staining time and this improved things (there is at leasat some pink now). Next he will try eosinol. Thanks again Yak-Nam On 8/18/08 3:07 PM, "Bryan Llewellyn" wrote: > It is sometimes possible to stain tissues with eosinol when they do not > stain with eosin Y. Eosinol is the free anion of eosin Y and is not soluble > in water. You can sometimes buy it, or can make it from a strong aqueous > solution of eosin Y by dropping in concentrated hydrochloric acid until > maximum precipitate is obtained, then filtering and washing several times > with distilled water to remove the acid. When dried, a small amount added > to the final xylene (yes, xylene) will colour the tissue. When dark enough, > coverslip. > > Bryan Llewellyn > > >>>> >>>>> Hello, >>>>> >>>>> I have a colleague with a staining problem. He has cells grown on >>>>> polymeric >>>>> scaffolds. He can not embedd in paraffin due to a mismatch in the >>>>> processing >>>>> and his scaffold material; he has thus embedded in OCT. >>>>> >>>>> This is his procedure: >>>>> Fix cell seeded scaffold in formalin (these are delicate samples >>>>> thus, fixing before embedding) >>>>> Cryoprotect in grade sucrose to 30% sucrose >>>>> Embed in OCT >>>>> Section at 6um >>>>> General H&E staining-using 1 min for Hematox and 1.5 min for Eosin >>>>> (following >>>>> one of Richard Allan suggested protocols). >>>>> >>>>> Problem: there is no eosin staining. The cells are just purple, >>>>> there is no pink at all. We are not sure why this is. Is this caused >>>>> by fixation? >>>>> >>>>> Does someone have any suggestions on how he can get the classic H&E >>>>> stain look for these samples? He has tried fresh solutions as well >>>>> as running other tissue (unfixed) through. It doesn't seem to be the >>>>> solutions used. >>>>> >>>>> Any suggestions would be appreciated. Thank you >>>>> Yak-Nam >>>>> >>>>> >>>>> Reserach Associate >>>>> CIMU >>>>> University of Washington >>>>> Box 355640 >>>>> Seattle, WA 98195 >>>>> >>>>> Tel.: >>>>> >>>>> >>>>> >>>>> _______________________________________________ >>>>> Histonet mailing list >>>>> Histonet@lists.utsouthwestern.edu >>>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>>> >>>> ..................................................................... >>>> : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : >>>> : Sr. Research Specialist University of Arizona : >>>> : (office: AHSC 4212) P.O. Box 245044 : >>>> : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : >>>> : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : >>>> :...................................................................: >>>> http://www.cba.arizona.edu/histology-lab.html >>>> >>>> >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet@lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Pat.Patterson <@t> propath.com Tue Aug 19 16:19:44 2008 From: Pat.Patterson <@t> propath.com (Pat Patterson) Date: Tue Aug 19 16:19:50 2008 Subject: [Histonet] Looking for a Histobath Freezer Message-ID: <82C7248978CB50469FD6BA68EBBEFE673473A1@exchange.propathlab.com> I'm looking for a Histobath Freezer that is in good operating condition. Please contact me directly at the email address given. Thanks! Pat Patterson Supervisor, Immunohistochemistry pat.patterson@propath.com ______________________________________________________________________________ This e-mail may contain confidential or privileged information. If you think you have received this e-mail in error, please advise the sender by reply e-mail and then delete this e-mail immediately. From jnocito <@t> satx.rr.com Tue Aug 19 16:33:21 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Aug 19 16:33:26 2008 Subject: [Histonet] Microwave safety References: <455370.95459.qm@web65701.mail.ac4.yahoo.com> Message-ID: this is my BIG beef. CAP insists on these questions, with the facade of safety concerns and runs with it. They're like law makers. They only make laws that doesn't affect them. My question is how many of the CAP has stock or some other private interest in these companies. Now for the good one (and I know I'm gonna get flamed on this one. This time, there is no CEO to call, so who has the last laugh now?) It's these microwave companies that lobby CAP, JACHO and OSHA in the "interest" of safety. Bull paddies!!! I've used household microwaves in the lab since the 1980's. Hell, Biogenex was touting antigen retrieval with lead thiocyanide using a microwave back in the late 80s and NOW, all of a sudden, it's a problem. Deer pebbles. My Knights of Columbus Council was having a taco sale at church one time. One of the members wrapped some tortillas in a towel to warm them up. He thought he set the timer for 2 minutes, but set it for 20 minutes. We went outside to sell our tacos. When we went back to the kitchen, the microwave was smoking and the towel was on fire. Almost set the church on fire. That's the only accident I had with a microwave. I've had more accidents using a hot plate than with a microwave. Here's where I date myself, I was heating up basic fuchsin making stock Mayer's mucicarmine solution. I walked away from the hot plate and forgot about it until I heard a POP. I ran back into the lab to find a nice magenta color all over the counter, ceiling and walls. The sad thing is that no one will confront CAP about this. I mean someone with clout. I may have a mouth, but I have no clout. A man has to know his limitations. We need to get together and petition CAP, but we won't get far because the pathologists own CAP. Now, let the flaming begin. JTT ----- Original Message ----- From: "Rene J Buesa" To: "Weems, Joyce" Cc: "histonet" Sent: Tuesday, August 19, 2008 10:28 AM Subject: RE: [Histonet] Microwave safety Yes, the train left the station long time ago, precisely the moment the CAP embraced this issue and decided to enforce it! Ren? J. --- On Tue, 8/19/08, Weems, Joyce wrote: From: Weems, Joyce Subject: RE: [Histonet] Microwave safety To: rjbuesa@yahoo.com Cc: "histonet" Date: Tuesday, August 19, 2008, 11:08 AM The sad thing is that we all know this, but it continues to be a growing issue in our country. What can we do to stop it? It's like a run-away train? j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, August 19, 2008 8:57 AM To: Joe Nocito; Anne van Binsbergen Cc: histonet Subject: Re: [Histonet] Microwave safety Joe and Anne: Have you both forgotten that every time a lab buys a cheap "home" microwave, the large lab equipment manufacturing companies lose a sale? Have you forgotten that if an accident happens (no matter how remote the possibility is) any "good claims attorney" will be able to twist the issue to "nail" somebody for using an equipment "against the manufacturer's specifications"? We are talking about monetary interests, not about personnel safety! Ren? J. --- On Tue, 8/19/08, Anne van Binsbergen wrote: From: Anne van Binsbergen Subject: Re: [Histonet] Microwave safety To: "Joe Nocito" Cc: "histonet" Date: Tuesday, August 19, 2008, 8:46 AM hey joe i have had accidents in my kitchen - but never in the lab im also getting a little tired of the CAP dictatorship - and we have not even started yet!!! if they were all dyed-in-the-wool histotechs with years and years of experience i would find it easier to accept - but then again if they had all that histo experience they would certainly not be expecting/demanding all these silly 'improvements' am also flipping annoyed that i am being told which EQA i can and cannot use - the list of CAP approved EQA facilities does not include NEQAS or RCPA QAP, both of which i use and am extremely satisfied with .... CAP are all about the money thats my vent for the decade whew....i feel better already!!!! abudhabiannie 2008/8/19 Joe Nocito > Histoland, > has anyone ever experienced or heard of an accident while using a "for home > use only" microwave? I have never had an accident. Before I go on another > tangent about CAP and OSHA, I'd like to know what everyone else experience > is. Since everyone is running around, changing the way they do things just > because CAP says breast cases have to be documented as to the length > of time > these cases have been fixing in formalin. Uh-oh, I feel it coming on. > Another tangent. I feel the flames warming up. > > JTT > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dmborel <@t> aol.com Tue Aug 19 16:41:58 2008 From: Dmborel <@t> aol.com (Dmborel@aol.com) Date: Tue Aug 19 16:42:17 2008 Subject: Fwd: [Histonet] PCR For NAILS Message-ID: I sent this on 8/18/08 and it has not posted yet, so I am sending it again on 8/19/08. ____________________________________ From: Dmborel To: aakrasht@yahoo.com Sent: 8/18/2008 3:33:21 P.M. Central Daylight Time Subj: Re: [Histonet] PCR For NAILS Here are numerous references, but I do not know if they contain actual procedures. Does anyone know of a reference lab in the US that performs these? Can they be done on formalin fixed paraffin embedded specimens? Molecular Biology Techniques for Identifying Dermatophytes and Their Possible Use in Diagnosing Onychomycosis in Human Toenail A Review Judith M. Binstock, PhD* Traditional methods of diagnosing onychomycosis, such as microscopy, histologic staining, and cultures, may not provide the clinician with documentation before initiating antifungal drug therapy. DNA technology now supplies the tools for increased sensitivity, speed, and accuracy in the diagnostic arena by allowing for the amplification, qualification, and quantitation of DNA. These techniques, already being used to identify many infectious agents, may soon be commonly applied to onychomycosis. This report reviews some of the DNA-based techniques that are currently being used to identify dermatophytes and their possible diagnostic use. (J Am Podiatr Med Assoc 97(2): 134-144, 2007) References 1. ELEWSKI BE: Onychomycosis: pathogenesis, diagnosis, and management. Clin Microbiol Rev 11: 415, 1998. 2. GHANNOUM MA, HAJJEH RA, SCHER R, ET AL: A large-scale North American study of fungal isolates from nails: the frequency of onychomycosis, fungal distribution, and antifungal susceptibility patterns. J Am Acad Dermatol 43: 641, 2000. 3. VANDER STRATEN MR, HOSSAIN MA, GHANNOUM MA: Cutaneous infections dermatophytosis, onychomycosis, and tinea versicolor. Infect Dis Clin North Am 17: 87, 2003. 4. WEITZMAN I, SUMMERBELL RC: The dermatophytes. Clin Microbiol Rev 8: 240, 1995. 5. JOUSSON O, LECHENNE B, BONTEMS O, ET AL: Multiplication of an ancestral gene encoding secreted fungalysin preceded species differentiation in the dermatophytes Trichophyton and Microsporum. Microbiology 150: 301, 2004. 6. JOUSSON O, LECHENNE B, BONTEMS O, ET AL: Secreted subtilisin gene family in Trichophyton rubrum. Gene 339: 79, 2004. 7. SCHER RK: Onychomycosis: therapeutic update. J Am Acad Dermatol 40: S21, 1999. 8. KARDJEVA V, SUMMERBELL R, KANTARDJIEV T, ET AL: Fortyeight- hour diagnosis of onychomycosis with subtyping of Trichophyton rubrum strains. J Clin Microbiol 44: 1419, 2006. 9. CRIBIER B, MENA ML, REY D, ET AL: Changes in patients infected with human immunodeficiency virus: a prospective controlled study. Arch Dermatol 134: 1216, 1998. 10. AKIBA H, MOTOKI Y, SATOH M, ET AL: Recalcitrant trichophytic granuloma associated with NK-cell deficiency in a SLE patient treated with corticosteroid. J Dermatol 11: 58, 2001. 11. RODRIGUEZ-SOTO ME, FERNANDEZ-ANDREU CM, MOYA DUQUE S, ET AL: Clinico-mycology study of onychomycosis in elderly patients. Rev Inst Med Trop Sao Paulo 35: 213, 1993. 12. National Diabetes Information Clearinghouse Web site. Available at: _http://diabetes.niddk.nih.gov/dm/pubs/statistics/_ (http://diabetes.niddk.nih.gov/dm/pubs/statistics/) index.htm#7. Accessed February 12, 2007. 13. GUPTA AK, KONNIKOV N, MACDONALD P, ET AL: Prevalence and epidemiology of toenail onychomycosis in diabetic subjects: a multicentre survey. Br J Dermatol 139: 665, 1998. 14. MULLIS KB, FALOONA FA: Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol 155: 335, 1987. 15. FOY CA, PARKES HC: Emerging homogeneous DNA-based technologies in the clinical laboratory. Clin Chem 47: 990, 2001. 16. HOLDANE DJ, ROBART E: A comparison of calcofluor white, potassium hydroxide, and culture for the laboratory diagnosis of superficial fungal infection. Diagn Microbiol Infect Dis 13: 337, 1990. 17. ELEWSKI BE: Diagnostic techniques for confirming onychomycosis. J Am Acad Dermatol 35: S6, 1996. 18. ZACHARIUS RM, ZELL TE, MORRISON JH, ET AL: Glycoprotein 142 March/April 2007 ? Vol 97 ? No 2 ? Journal of the American Podiatric Medical Association Celebrating100years of continuous publication:1907?2007 staining following electrophoresis on acrylamide gels. Anal Biochem 30: 148, 1969. 19. MAYNARD JA, IPPOLITO EG, PONSETI IV, ET AL: Histochemistry and ultrastructure of the growth plate in metaphyseal dysostosis: further observations on the structure of the cartilage matrix. J Pediatr Orthop 1: 161, 1981. 20. STROMQVIST M, GRUFFMAN H: Periodic acid/Schiff staining of glycoproteins immobilized on a blotting matrix. Biotechniques 13: 744, 1992. 21. KAPPE R, RIMEK D: Diagnosis of fungal diseases. Prog Drug Res Spec No: 39, 2003. 22. ZABAWSKI EJ JR, STYLES AR, COCKERELL CJ: Routine periodic acid-Schiff staining of nail plate fragments in fungal cultures for onychomycosis: a method to increase the sensitivity of diagnosis. Cutis 66: 456, 2000. 23. WEINBERG JM, KOESTENBLATT EK, JENNINGS MB: Utility of histopathologic analysis in the evaluation of onychomycosis. JAPMA 95: 258, 2005. 24. LAWRY MA, HANEKE E, STROBECK K, ET AL: Methods for diagnosing onychomycosis: a comparative study and review of the literature. Arch Dermatol 136: 1112, 2000. 25. BOREK PP, ALBRESDKI DA, PROBOLUS JA, ET AL: New methods with potassium hydroxide for diagnosing onychomycosis. Am J Dermatopathol 21: 90, 1999. 26. WEINBERG JM, KOESTENBLATT EK, TUTRONE WD, ET AL: Comparison of diagnostic methods in the evaluation of onychomycosis. J Am Acad Dermatol 49: 193, 2003. 27. SINGH S, BEENA PM: Comparative study of different microscopic techniques and culture media for the isolation of dermatophytes. Indian J Med Microbiol 21: 21, 2003. 28. ELEWSKI BE, EL CHARIF M, COOPER KD, ET AL: Reactivity to trichophytin antigen in patients with onychomycosis: effect of terbinafine. J Am Acad Dermatol 46: 371, 2002. 29. YEO SF, WONG B: Current status of nonculture methods for diagnosis of invasive fungal infections. Clin Microbiol Rev 15: 465, 2002. 30. LOFFLER J, HEBART H, SEPE S, ET AL: Detection of PCRamplified fungal DNA by using a PCR-ELISA system. Med Mycol 36: 275, 1998. 31. SUMMERBELL RC, COOPER E, BUNN U, ET AL: Onychomycosis: a critical study of techniques and criteria for confirming the etiologic significance of nondermatophytes. Med Mycol 43: 39, 2005. 32. SAMDANI AJ, DYKES PJ, MARKS R: The proteolytic activity of strains of T. mentagrophytes and T. rubrum isolated from tinea pedis and tinea unguium infections. J Med Vet Mycol 33: 167, 1995. 33. VERSALOVIC J, KOEUTH T, LUPSKI JR: Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res 19: 6823, 1991. 34. Human Genome Project information: DNA forensics. Available at: _http://www.ornl.gov/sci/techresources/Human_ (http://www.ornl.gov/sci/techresources/Human) _ Genome/elsi/forensics.shtml. Accessed February 9, 2007. 35. GINZINGER DG: Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream. Exp Hematol 30: 503, 2002. 36. ULRICHS T, LEFMANN M, REICH M, ET AL: Modified immunohistological staining allows detection of Ziehl-Neelsennegative Mycobacterium tuberculosis organisms and their precise localization in human tissue. J Pathol 205: 633, 2005. 37. MACKAY IM, ARDEN KE, NITSCHE A: Real-time PCR in virology. Nucleic Acids Res 30: 1292, 2002. 38. PACHECO N, MAGO V, GOMEZ I, ET AL: Comparison of PCR and common clinical tests for the diagnosis of H. pylori in dyspeptic patients. Diagn Microbiol Infect Dis 39: 207, 2001. 39. NOWAKOWSKI J, SCHWARTZ I, LIVERIS D, ET AL: Laboratory diagnostic techniques for patients with early Lyme disease associated with erythema migrans: a comparison of different techniques. Clin Infect Dis 33: 2023, 2001. 40. GLENNON M, CORMICAN M: Detection and diagnosis of mycobacterial pathogens using PCR. Expert Rev Mol Diagn 1: 163, 2001. 41. MOHAMMADI T, PIETERSZ RN, VANDENBROUCKE-GRAULS CM, ET AL: Detection of bacteria in platelet concentrates: comparison of broad-range real-time 16S rDNA polymerase chain reaction and automated culturing. Transfusion 45: 731, 2005. 42. BALESTRINO D, HAAGENSEN JA, RICH C, ET AL: Characterization of type 2 quorum sensing in Klebsiella pneumoniae and relationship with biofilm formation. J Bacteriol 187: 2870, 2005. 43. PILCHER CD, MCPHERSON JT, LEONE PA, ET AL: Real-time, universal screening for acute HIV infection in a routine HIV counseling and testing population. JAMA 288: 216, 2002. 44. HESS C, KLIMKAIT T, SCHLAPBACH L, ET AL: Association of a pool of HIV-1 with erythrocytes in vivo: a cohort study. Lancet 359: 2230, 2002. 45. CALVARIO A, BOZZI A, SCARASCIULLI M, ET AL: Herpes consensus PCR test: a useful diagnostic approach to the screening of viral diseases of the central nervous system. J Clin Virol 25 (suppl): 71, 2002. 46. PRADO I, ROSARIO D, BERNARDO L, ET AL: PCR detection of dengue virus using dried whole blood spotted on filter paper. J Virol Methods 125: 75, 2005. 47. RADFORD SA, JOHNSON EM, LEEMING JP, ET AL: Molecular epidemiological study of Aspergillus fumigatus in a bone marrow transplantation unit by PCR amplification of ribosomal intergenic spacer sequences. J Clin Microbiol 36: 1294, 1998. 48. GRASER Y, EL FARI M, PRESBER W, ET AL: Identification of common dermatophytes (Trichophyton, Microsporum, Epidermophyton) using polymerase chain reactions. Br J Dermatol 138: 5762, 1998. 49. VELEGRAKI A, KAMBOURIS M, KOSTOUROU A, ET AL: Rapid extraction of fungal DNA from clinical samples for PCR amplification. Med Mycol 37: 69, 1999. 50. FERRER C, COLOM F, FRASES S, ET AL: Detection and identification of fungal pathogens by PCR and by ITS2 and 5.8S ribosomal DNA typing in ocular infections. J Clin Microbiol 39: 2873, 2001. 51. LI RY, LI DM, YU J, ET AL: Application of molecular biology techniques in the identification of pathogenic fungi and the diagnosis of fungal infection. Beijing Da Xue Xue Bao 36: 536, 2004. 52. HEID CA, STEVENS J, LIVAK KJ, ET AL: Real time quantitative PCR. Genome Res 6: 986, 1996. 53. ORLANDO C, PINZANI P, PAZZAGLI M: Developments in quantitative PCR. Clin Chem Lab Med 36: 255, 1998. 54. WITTWER CT, RIRIE KM, ANDREW RV, ET AL: The LightCycler: a microvolume multisample fluorimeter with rapid temperature control. Biotechniques 22: 176, 1997. 55. GUTZMER R, MOMMERT S, KUTTLER U, ET AL: Rapid identification and differentiation of fungal DNA in dermatological specimens by LightCycler PCR. J Med Microbiol 53: 1207, 2004. Journal of the American Podiatric Medical Association ? Vol 97 ? No 2 ? March/April 2007 143 Celebrating100years of continuous publication:1907?2007 56. TSUBOI R, OKEKE CN, INOUE A, ET AL: Identification and viability assessment of dermatophytes infecting nail based on quantitative PCR of dermatophyte actin (ACT) mRNA. Nippon Ishinkin Gakkai Zasshi 43: 91, 2002. 57. SAVOLAINEN V, COWAN RS, VOGLER AP, ET AL: Towards writing the encyclopedia of life: an introduction to DNA barcoding. Philos Trans R Soc Lond B Biol Sci 360: 1805, 2005. 58. SUMMERBELL RC, LEVESQUE CA, SEIFERT KA, ET AL: Microcoding: the second step in DNA barcoding. Philos Trans R Soc Lond B Biol Sci 360: 1897, 2005. 59. KANO R, NAKAMURA Y, WATARI T, ET AL: Phylogenetic analysis of 8 dermatophyte species using chitin synthase 1 gene sequences. Mycoses 40: 411, 1997. 60. GRASER Y, EL FARI M, VILGALYS R, ET AL: Phylogeny and taxonomy of the family Arthrodermataceae (dermatophytes) using sequence analysis of the ribosomal ITS region. Med Mycol 37: 105, 1999. 61. GRASER Y, KUIJPERS AF, PRESBER W, ET AL: Molecular taxonomy of Trichophyton mentagrophytes and T. tonsurans. Med Mycol 37: 315, 1999. 62. KANO R, NAKAMURA Y, WATARI T, ET AL: Phylogenetic relation of Epidermophyton floccosum to the species of Microsporum and Trichophyton in chitin synthase 1 (CHS1) gene sequences. Mycopathologia 146: 111, 1999. 63. MAKIMURA K, TAMURA Y, MOCHIZUKI T, ET AL: Phylogenetic classification and species identification of dermatophyte strains based on DNA sequences of nuclear ribosomal internal transcribed spacer 1 regions. J Clin Microbiol 37: 920, 1999. 64. MOCHIZUKI T, KAWASAKI M, ISHIZAKI H, ET AL: Molecular epidemiology of Arthroderma benhamiae, an emerging pathogen of dermatophytoses in Japan, by polymorphisms of the non-transcribed spacer region of the ribosomal DNA. J Dermatol Sci 27: 14, 2001. 65. OHST T, DE HOOG S, PRESBER W, ET AL: Origins of microsatellite diversity in the Trichophyton rubrum?T. violaceum clade (dermatophytes). J Clin Microbiol 42: 4444, 2004. 66. BOCK M, NICKEL P, MAIWALD M, ET AL: Diagnosis of dermatomycoses with polymerase chain reaction. Hautarzt 48: 175, 1997. 67. BAEK SC, CHAE HJ, HOUH D, ET AL: Detection and differentiation of causative fungi of onychomycosis using PCR amplification and restriction enzyme analysis. Int J Dermatol 37: 682, 1998. 68. EL FARI M, TIETZ HJ, PRESBER W, ET AL: Development of an oligonucleotide probe specific for Trichophyton rubrum. Br J Dermatol 141: 240, 1999. 69. TURIN L, RIVA F, GALBIATI G, ET AL: Fast, simple and highly sensitive double-rounded polymerase chain reaction assay to detect medically relevant fungi in dermatological specimens. Eur J Clin Invest 30: 511, 2000. 70. MACHOUART-DUBACH M, LACROIX C, DE CHAUVIN MF, ET AL: Rapid discrimination among dermatophytes, Scytalidium spp., and other fungi with a PCR-restriction fragment length polymorphism ribotyping method. J Clin Microbiol 39: 685, 2001. 71. ARCA E, SARACLI MA, AKAR A, ET AL: Polymerase chain reaction in the diagnosis of onychomycosis. Eur J Dermatol 14: 52, 2004. 72. HOWELL SA, BARNARD RJ, HUMPHREYS F: Application of molecular typing methods to dermatophyte species that cause skin and nail infections. J Med Microbiol 48: 33, 1999. 73. MOCHIZUKI T, SUGIE N, UEHARA M: Random amplification of polymorphic DNA is useful for the differentiation of several anthropophilic dermatophytes. Mycoses 40: 405, 1997. 74. KIM JA, TAKAHASHI Y, TANAKA R, ET AL: Identification and subtyping of Trichophyton mentagrophytes by random amplified polymorphic DNA. Mycoses 44: 157, 2001. 75. LIU D, PEARCE L, LILLEY G, ET AL: PCR identification of dermatophyte fungi Trichophyton rubrum, T. soudanense and T. gourvilii. J Med Microbiol 51: 117, 2002. 76. NISHIO K, KAWASAKI M, ISHIZAKI H: Phylogeny of the genera Trichophyton using mitochondrial DNA analysis. Mycopathologia 117: 127, 1992. 77. SU CS, MEYER SA: Characterization of mitochondrial DNA in various Candida species: isolation, restriction endonuclease analysis, size, and base composition. Int J Syst Bacteriol 41: 6, 1991. 78. OKEKE CN, TSUBOI R, KAWAI M, ET AL: Isolation of an intron- containing partial sequence of the gene encoding dermatophyte actin (ACT) and detection of a fragment of the transcript by reverse transcription-nested PCR as a means of assessing the viability of dermatophytes in skin scales. J Clin Microbiol 39: 101, 2001. 79. NIMURA K, NIWANO Y, ISHIDUKA S, ET AL: Actin gene-targeted RT-PCR could be a useful method for evaluating in vitro fungicidal activity against dermatophytes. J Int Med Res 31: 407, 2003. 80. KANO R, NAKAMURA Y, WATARI T, ET AL: Molecular analysis of chitin synthase 1 (CHS1) gene sequences of Trichophyton mentagrophytes complex and T. rubrum. Curr Microbiol 37: 236, 1998. 81. KANO R, NAKAMURA Y, WATARI T, ET AL: Species-specific primers of chitin synthase 1 gene for the differentiation of the Trichophyton mentagrophytes complex. Mycoses 42: 71, 1999. 82. KANBE T, SUZUKI Y, KAMIYA A, ET AL: PCR-based identification of common dermatophyte species using primer sets specific for the DNA topoisomerase II genes. J Dermatol Sci 32: 151, 2003. 83. KANBE T, SUZUKI Y, KAMIYA A, ET AL: Species-identification of dermatophytes Trichophyton, Microsporum and Epidermophyton by PCR and PCR-RFLP targeting of the DNA topoisomerase II genes. J Dermatol Sci 33: 41, 2003. 84. JACKSON CJ, BARTON RC, EVANS EG: Species identification and strain differentiation of dermatophyte fungi by analysis of ribosomal-DNA intergenic spacer regions. J Clin Microbiol 37: 931, 1999. 85. JACKSON CJ, BARTON RC, KELLY SL, ET AL: Strain identification of Trichophyton rubrum by specific amplification of subrepeat elements in the ribosomal DNA nontranscribed spacer. J Clin Microbiol 38: 4527, 2000. 86. GUPTA AK, KOHLI Y, SUMMERBELL RC: Variation in restriction fragment length polymorphisms among serial isolates from patients with Trichophyton rubrum infection. J Clin Microbiol 39: 3260, 2001. 87. BOCK M, MAIWALD M, KAPPE R, ET AL: Polymerase chain reaction-based detection of dermatophyte DNA with a fungus-specific primer system. Mycoses 37: 79, 1994. 88. KAPPE R, FAUSER C, OKEKE CN, ET AL: Universal fungusspecific primer systems and group-specific hybridization oligonucleotides for 18S rDNA. Mycoses 39: 25, 1996. 89. KAPPE R, OKEKE CN, FAUSER C, ET AL: Molecular probes for the detection of pathogenic fungi in the presence of human tissue. J Med Microbiol 47: 811, 1998. 144 March/April 2007 ? Vol 97 ? No 2 ? Journal of the American Podiatric Medical Association Celebrating100years of continuous publication:1907?2007 90. SUMMERBELL RC, HAUGLAND RA, LI A, ET AL: rRNA gene internal transcribed spacer 1 and 2 sequences of asexual, anthropophilic dermatophytes related to Trichophyton rubrum. J Clin Microbiol 37: 4005, 1999. 91. GAEDIGK A, GAEDIGK R, ABDEL-RAHMAN SM: Genetic heterogeneity in the rRNA gene locus of Trichophyton tonsurans. J Clin Microbiol 41: 5478, 2003. 92. YAZDANPARAST A, JACKSON CJ, BARTON RC, ET AL: Molecular strain typing of Trichophyton rubrum indicates multiple strain involvement in onychomycosis. Br J Dermatol 148: 51, 2003. 93. SHIN JH, SUNG JH, PARK SJ, ET AL: Species identification and strain differentiation of dermatophyte fungi using polymerase chain reaction amplification and restriction enzyme analysis. J Am Acad Dermatol 48: 857, 2003. 94. MOCHIZUKI T, TANABE H, KAWASAKI M, ET AL: Rapid identification of Trichophyton tonsurans by PCR-RFLP analysis of ribosomal DNA regions. J Dermatol Sci 32: 25, 2003. 95. MOCHIZUKI T, ISHIZAKI H, BARTON RC, ET AL: Restriction fragment length polymorphism analysis of ribosomal DNA intergenic regions is useful for differentiating strains of Trichophyton mentagrophytes. J Clin Microbiol 41: 4583, 2003. 96. WHITE TJ, BRUNS T, LEE S, ET AL: ?Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics,? in PCR Protocols, ed by MA Innes, GH Gelfand, JS Sninsky, et al, p 315, Academic Press, London, 1990. 97. KAMIYA A, KIKUCHI A, TOMITA Y, ET AL: PCR and PCR-RFLP techniques targeting the DNA topoisomerase II gene for rapid clinical diagnosis of the etiologic agent of dermatophytosis. J Dermatol Sci 34: 35, 2004. 98. RAD MM, JACKSON C, BARTON RC, ET AL: Single strains of Trichophyton rubrum in cases of tinea pedis. J Med Microbiol 54: 725, 2005. 99. KANO R, HIRAI A, MURAMATSU M: Direct detection of dermatophytes in skin samples based on sequences of the chitin synthase 1 (CHS1) gene. J Vet Med Sci 65: 267, 2003. 100. POUNDER JI, WILLIAMS S, HANSEN D, ET AL: Repetitive-sequence- PCR-based DNA fingerprinting using the Diversilab system for identification of commonly encountered dermatophytes. J Clin Microbiol 43: 2141, 2005. 101. LEMONT H: Pathologic and diagnostic considerations in onychomycosis. JAPMA 87: 498, 1997. 102. GUPTA AK, JAIN HC, LYNDE CW, ET AL: Prevalence and epidemiology of unsuspected onychomycosis in patients visiting dermatologists? offices in Ontario, Canada: a multicenter survey of 2001 patients. Int J Dermatol 36: 783, 1997. 103. FLETCHER CL, HAY RJ, SMEETON NC: Onychomycosis: the development of a clinical diagnostic aid for toenail disease: part I. Establishing discriminating historical and clinical features. Br J Dermatol 150: 701, 2004. 104. LIU D, COLOE S, BAIRD R, ET AL: Application of PCR to the identification of dermatophyte fungi. J Med Microbiol 49: 493, 2000. 105. BELEC L, AUTHIS J, ELIEZER-VANEROT MC, ET AL: Myoglobin as a polymerase chain reaction (PCR) inhibitor: a limitation for PCR from skeletal muscle tissue avoided by the use of Thermus thermophilus polymerase. Muscle Nerve 21: 1064, 1998. 106. GUPTA AK, DANIEL CR III: Factors that may affect the response of onychomycosis to oral antifungal therapy. Australas J Dermatol 39: 222, 1998. 107. DRAKE LA, SCHER RK, SMITH EB, ET AL: Effect of onychomycosis on quality of life. J Am Acad Dermatol 38: 702, 1998. 108. MOLDEN E, GARCIA BH, BRAATHEN P, ET AL: Co-prescription of cytochrome P450 2D6/3A4 inhibitor-substrate pairs in clinical practice: a retrospective analysis of data from Norwegian primary pharmacies. Eur J Clin Pharmacol 61: 119, 2005. 109. BREUER K, VOLKER B, GUTZMER R, ET AL: Facial pigmentation following therapy with terbinafine. Hautarzt 56: 1056, 2005. 110. US FOOD AND DRUG ADMINISTRATION: FDA Talk Paper: FDA Issues Health Advisory Regarding the Safety of Sporanox Products and Lamisil Tablets to Treat Fungal Infections, US Food and Drug Administration, Rockville, MD, May 9, 2001. 111. Projected population of the United States, by age and sex: 2000 to 2050. Available at: _http://www.census.gov/ipc/_ (http://www.census.gov/ipc/) www/usinterimproj/natprojtab02a.xls. Accessed February 6, 2007. 112. LEVY LA: Epidemiology of onychomycosis in special-risk populations. JAPMA 87: 546, 1997. David M. Borel, M.D. Dermatopathology Diagnostics Topeka, Kansas In a message dated 8/17/2008 12:54:56 P.M. Central Daylight Time, aakrasht@yahoo.com writes: Hi There Anyone knows how to do PCR for nails (Histology) and what is the proceedure! Thanks for your help. Ali _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________ Looking for a car that's sporty, fun and fits in your budget? _Read reviews on AOL Autos_ (http://autos.aol.com/cars-Volkswagen-Jetta-2009/expert-review?ncid=aolaut00030000000007) . **************It's only a deal if it's where you want to go. Find your travel deal here. (http://information.travel.aol.com/deals?ncid=aoltrv00050000000047) From carlos <@t> clubstaffing.com Tue Aug 19 16:42:25 2008 From: carlos <@t> clubstaffing.com (Carlos Stolk) Date: Tue Aug 19 16:42:30 2008 Subject: [Histonet] Microwave safety In-Reply-To: References: <455370.95459.qm@web65701.mail.ac4.yahoo.com> Message-ID: This is getting a little bit complicated.. I think we should talk about this over a nice steak dinner. Carlos Stolk Account Representative Georgia | New Mexico South Carolina| Texas Phone: 800-875-8999 ext. 258 Fax: 561-367-0884 carlos@clubstaffing.com Club Staffing, Inc. 5901 Broken Sound Pkwy, Ste 500 Boca Raton, FL 33487 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Tuesday, August 19, 2008 5:33 PM To: rjbuesa@yahoo.com; Weems, Joyce Cc: histonet Subject: Re: [Histonet] Microwave safety this is my BIG beef. CAP insists on these questions, with the facade of safety concerns and runs with it. They're like law makers. They only make laws that doesn't affect them. My question is how many of the CAP has stock or some other private interest in these companies. Now for the good one (and I know I'm gonna get flamed on this one. This time, there is no CEO to call, so who has the last laugh now?) It's these microwave companies that lobby CAP, JACHO and OSHA in the "interest" of safety. Bull paddies!!! I've used household microwaves in the lab since the 1980's. Hell, Biogenex was touting antigen retrieval with lead thiocyanide using a microwave back in the late 80s and NOW, all of a sudden, it's a problem. Deer pebbles. My Knights of Columbus Council was having a taco sale at church one time. One of the members wrapped some tortillas in a towel to warm them up. He thought he set the timer for 2 minutes, but set it for 20 minutes. We went outside to sell our tacos. When we went back to the kitchen, the microwave was smoking and the towel was on fire. Almost set the church on fire. That's the only accident I had with a microwave. I've had more accidents using a hot plate than with a microwave. Here's where I date myself, I was heating up basic fuchsin making stock Mayer's mucicarmine solution. I walked away from the hot plate and forgot about it until I heard a POP. I ran back into the lab to find a nice magenta color all over the counter, ceiling and walls. The sad thing is that no one will confront CAP about this. I mean someone with clout. I may have a mouth, but I have no clout. A man has to know his limitations. We need to get together and petition CAP, but we won't get far because the pathologists own CAP. Now, let the flaming begin. JTT ----- Original Message ----- From: "Rene J Buesa" To: "Weems, Joyce" Cc: "histonet" Sent: Tuesday, August 19, 2008 10:28 AM Subject: RE: [Histonet] Microwave safety Yes, the train left the station long time ago, precisely the moment the CAP embraced this issue and decided to enforce it! Ren? J. --- On Tue, 8/19/08, Weems, Joyce wrote: From: Weems, Joyce Subject: RE: [Histonet] Microwave safety To: rjbuesa@yahoo.com Cc: "histonet" Date: Tuesday, August 19, 2008, 11:08 AM The sad thing is that we all know this, but it continues to be a growing issue in our country. What can we do to stop it? It's like a run-away train? j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, August 19, 2008 8:57 AM To: Joe Nocito; Anne van Binsbergen Cc: histonet Subject: Re: [Histonet] Microwave safety Joe and Anne: Have you both forgotten that every time a lab buys a cheap "home" microwave, the large lab equipment manufacturing companies lose a sale? Have you forgotten that if an accident happens (no matter how remote the possibility is) any "good claims attorney" will be able to twist the issue to "nail" somebody for using an equipment "against the manufacturer's specifications"? We are talking about monetary interests, not about personnel safety! Ren? J. --- On Tue, 8/19/08, Anne van Binsbergen wrote: From: Anne van Binsbergen Subject: Re: [Histonet] Microwave safety To: "Joe Nocito" Cc: "histonet" Date: Tuesday, August 19, 2008, 8:46 AM hey joe i have had accidents in my kitchen - but never in the lab im also getting a little tired of the CAP dictatorship - and we have not even started yet!!! if they were all dyed-in-the-wool histotechs with years and years of experience i would find it easier to accept - but then again if they had all that histo experience they would certainly not be expecting/demanding all these silly 'improvements' am also flipping annoyed that i am being told which EQA i can and cannot use - the list of CAP approved EQA facilities does not include NEQAS or RCPA QAP, both of which i use and am extremely satisfied with .... CAP are all about the money thats my vent for the decade whew....i feel better already!!!! abudhabiannie 2008/8/19 Joe Nocito > Histoland, > has anyone ever experienced or heard of an accident while using a "for home > use only" microwave? I have never had an accident. Before I go on another > tangent about CAP and OSHA, I'd like to know what everyone else experience > is. Since everyone is running around, changing the way they do things just > because CAP says breast cases have to be documented as to the length > of time > these cases have been fixing in formalin. Uh-oh, I feel it coming on. > Another tangent. I feel the flames warming up. > > JTT > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Tue Aug 19 16:45:38 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Aug 19 16:45:42 2008 Subject: [Histonet] tissue container disposal References: <802416.53857.qm@web65712.mail.ac4.yahoo.com> Message-ID: Most definitely. Some stated vary from county to county. It's always best to contact your local authorities. We throw our empty containers in the trash, but all our trash is incinerated. My last job, we collected the empty containers in a biohazard box to be picked up by a licensed waste company. JTT ----- Original Message ----- From: "Carlos Stolk" To: ; ; "sotiris lakis" Sent: Tuesday, August 19, 2008 10:49 AM Subject: RE: [Histonet] tissue container disposal > Are there are different rules and regulations that determine what is and > what is not legal in any given state/hospital? > > Carlos Stolk > Account Representative > Georgia | New Mexico > South Carolina| Texas > Phone: 800-875-8999 ext. 258 > Fax: 561-367-0884 > carlos@clubstaffing.com > Club Staffing, Inc. > 5901 Broken Sound Pkwy, Ste 500 > Boca Raton, FL 33487 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J > Buesa > Sent: Tuesday, August 19, 2008 11:33 AM > To: histonet@lists.utsouthwestern.edu; sotiris lakis > Subject: Re: [Histonet] tissue container disposal > > > > > > > The formalin in the used containers have to be emptied in the large > container for formalin to be removed by a specialized contractor. > The containers can be either incinerated (if plastic) or given to the same > contractor for disposal. A used container cannot be disposed off with the > formalin inside. > There are some variations by state and hospital. > Ren? J. > > --- On Tue, 8/19/08, sotiris lakis wrote: > > From: sotiris lakis > Subject: [Histonet] tissue container disposal > To: histonet@lists.utsouthwestern.edu > Date: Tuesday, August 19, 2008, 11:01 AM > > Hello everybody, > > I know disposal policies might differ between countries but we seem to be > facing a dead end concerning used empty tissue containers with > formaldehyde > remnants. We are provided with a toxic waste container for liquids and > special > bags for biohazardous material. There seems to be some confusion among > colleagues so I would appreciate it if someone could give us an idea about > the > best way to solve the problem. > > Thanks > S. Lakis > Resident in Pathology > > > > --------------------------------- > ?????????????? Yahoo! > ?????????? ?? ?????????? ???? ???? (spam); ?? > Yahoo! Mail ???????? ??? ???????? ?????? > ????????? ???? ??? ??????????? ????????? > http://login.yahoo.com/config/mail?.intl=gr > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -------------------------------------------------------------------------------- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lcrosby <@t> echelon-inc.com Tue Aug 19 17:29:55 2008 From: lcrosby <@t> echelon-inc.com (Lee Crosby) Date: Tue Aug 19 17:31:41 2008 Subject: FW: [Histonet] Help with IF in Chamber slide Message-ID: <000e01c9024b$1b6eaa90$be00000a@CPU360> Karen, I have always used glass chamber slides. I used NIH-3T3 and 3T3-L1 cells in DMEM. Each well received 200ul of cells with media. All volumes were 200 ul. I let them set 2 days and then followed this procedure: 1-Fix cells with 200 ul of paraformaldehyde added to the media (or replace media with 200 ul of neutral buffered formalin) and incubate for 20 min at room temp. 2-Wash 3 times with Tris buffered saline (TBS) 3-Permabilize the cells with 0.05% Triton X-100 at RT for 15 min. 4-Wash 3 times with TBS 5-Block with 10% goat serum in TBS 30 min at 37 Celsius 6-Add antibody (anti-PIP3 Echelon Biosciences Z-P345b) and incubate at room temp for 60 min 7-Wash 3 times in 1% goat serum in TBS 8-Add secondary (goat anti mouse IgM Jackson ImmunoResearch 115-065-075) 30 min at 37 Celsius 9-Wash 3 times in 1% goat serum in TBS 10-Add label (Strept-AlexaFluor 488 Mol Probes S-11223 1:2000) and incubate 30 min at 37 Celsius 11-Rinse thoroughly with distilled water 12-Dry completely 13-Seal with Prolong (Molecular Probes) and store overnight at 4 Celsius 14-Seal edges with your sweetheart's old nail polish 14-View with microscope We visualized PI(3,4,5)P3 in the cell membranes. So it should work for your protein as well. If your protein is on the outside of the membrane then you can eliminate step 3. Good luck. Lee Crosby Echelon Biosciences Inc. 801-588-0455 ext 329 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of karen Cai Sent: Tuesday, August 19, 2008 10:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help with IF in Chamber slide Hello, I need to do the IF the 8 well Lab-tek chamber slides. Does anybody have the experience of this? How to culture the cells before blocking? Is glass type good or using permanox? The cell I used is CHO cell. Membrane protein. Thanks in advance, Best, Karen No virus found in this outgoing message. Checked by AVG. Version: 7.5.524 / Virus Database: 270.6.5/1620 - Release Date: 8/19/2008 6:04 AM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Tue Aug 19 19:01:34 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Aug 19 19:01:51 2008 Subject: [Histonet] in situ question In-Reply-To: Message-ID: Some questions, What is "PARA"? Is it an something you add to the formaldehyde solution? Please don't use the term 4% paraformaldehyde, use 4% formaldehyde, unless you are rolling the tissue in a powder consisting of 4% paraformaldehyde and 96% talcum powder or some other solid! Please refer to: Manoonkitiwongsa & Schultz (2002) Histochem J 34: 365-367 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of N Fournier Sent: Wednesday, 20 August 2008 3:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] in situ question Hi, I have a few questions regarding in situ hybridization. The protocol that I am following indicates that they perfused rats with PBS followed by 4% paraformaledhyde (in PBS). However, I always thought that one generally extracts the rat brain fresh, flash freeze, section, and then post-fix in 4% paraformaldehyde before commencing with the in situ hybridization. I suppose it might not matter since in the end the tissue is post-fixed in PARA; however, I would like to hear what others with experience doing in situ think about this procedure. Additionally, does one always use DEPC treated solutions even for these perfusion steps (e.g., PBS perfusion, paraformaldehyde perfusion)? Lastly, does one have to section on a cryostat? Could I simply use a vibrating microtome to section the tissue instead. Thanks for the help everyone, Neil _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From lcrosby <@t> echelon-inc.com Tue Aug 19 20:06:54 2008 From: lcrosby <@t> echelon-inc.com (Lee Crosby) Date: Tue Aug 19 20:08:36 2008 Subject: FW: [Histonet] in situ question Message-ID: <001301c90261$093fcff0$be00000a@CPU360> Paraformaldehyde: Fisher item 04042-500 dissolved in water at 4% concentration. It is not formaldehyde. And I would suggest using neutral buffered formalin because dissolving parafomaldehyde is smelly, time consuming, and tricky. Lee Crosby Echelon Biosciences Inc. 801-588-0455 ext 329 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Tuesday, August 19, 2008 6:02 PM To: N Fournier; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] in situ question Some questions, What is "PARA"? Is it an something you add to the formaldehyde solution? Please don't use the term 4% paraformaldehyde, use 4% formaldehyde, unless you are rolling the tissue in a powder consisting of 4% paraformaldehyde and 96% talcum powder or some other solid! Please refer to: Manoonkitiwongsa & Schultz (2002) Histochem J 34: 365-367 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of N Fournier Sent: Wednesday, 20 August 2008 3:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] in situ question Hi, I have a few questions regarding in situ hybridization. The protocol that I am following indicates that they perfused rats with PBS followed by 4% paraformaledhyde (in PBS). However, I always thought that one generally extracts the rat brain fresh, flash freeze, section, and then post-fix in 4% paraformaldehyde before commencing with the in situ hybridization. I suppose it might not matter since in the end the tissue is post-fixed in PARA; however, I would like to hear what others with experience doing in situ think about this procedure. Additionally, does one always use DEPC treated solutions even for these perfusion steps (e.g., PBS perfusion, paraformaldehyde perfusion)? Lastly, does one have to section on a cryostat? Could I simply use a vibrating microtome to section the tissue instead. Thanks for the help everyone, Neil _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Tue Aug 19 20:41:36 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Aug 19 20:41:41 2008 Subject: [Histonet] in situ question In-Reply-To: <001301c90261$093fcff0$be00000a@CPU360> Message-ID: Which the becomes 4% formaldehyde or equivalent to 10% formalin. Not 4% paraformaldehyde. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee Crosby Sent: Wednesday, 20 August 2008 11:07 AM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] in situ question Paraformaldehyde: Fisher item 04042-500 dissolved in water at 4% concentration. It is not formaldehyde. And I would suggest using neutral buffered formalin because dissolving parafomaldehyde is smelly, time consuming, and tricky. Lee Crosby Echelon Biosciences Inc. 801-588-0455 ext 329 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: Tuesday, August 19, 2008 6:02 PM To: N Fournier; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] in situ question Some questions, What is "PARA"? Is it an something you add to the formaldehyde solution? Please don't use the term 4% paraformaldehyde, use 4% formaldehyde, unless you are rolling the tissue in a powder consisting of 4% paraformaldehyde and 96% talcum powder or some other solid! Please refer to: Manoonkitiwongsa & Schultz (2002) Histochem J 34: 365-367 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of N Fournier Sent: Wednesday, 20 August 2008 3:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] in situ question Hi, I have a few questions regarding in situ hybridization. The protocol that I am following indicates that they perfused rats with PBS followed by 4% paraformaledhyde (in PBS). However, I always thought that one generally extracts the rat brain fresh, flash freeze, section, and then post-fix in 4% paraformaldehyde before commencing with the in situ hybridization. I suppose it might not matter since in the end the tissue is post-fixed in PARA; however, I would like to hear what others with experience doing in situ think about this procedure. Additionally, does one always use DEPC treated solutions even for these perfusion steps (e.g., PBS perfusion, paraformaldehyde perfusion)? Lastly, does one have to section on a cryostat? Could I simply use a vibrating microtome to section the tissue instead. Thanks for the help everyone, Neil _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From marktarango <@t> gmail.com Tue Aug 19 23:24:41 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Tue Aug 19 23:24:47 2008 Subject: [Histonet] tissue container disposal In-Reply-To: <975537.88832.qm@web23004.mail.ird.yahoo.com> References: <975537.88832.qm@web23004.mail.ird.yahoo.com> Message-ID: <5b6eb13e0808192124x2c8ffcf9wf44fae1b1f491d40@mail.gmail.com> Someone needs to empty them out, rinse, and start recycling these things. It's killing the planet. Mark 2008/8/19 sotiris lakis : > Hello everybody, > > I know disposal policies might differ between countries but we seem to be facing a dead end concerning used empty tissue containers with formaldehyde remnants. We are provided with a toxic waste container for liquids and special bags for biohazardous material. There seems to be some confusion among colleagues so I would appreciate it if someone could give us an idea about the best way to solve the problem. > > Thanks > S. Lakis > Resident in Pathology > > > > --------------------------------- > ?????????????? Yahoo! > ?????????? ?? ?????????? ???? ???? (spam); ?? Yahoo! Mail ???????? ??? ???????? ?????? ????????? ???? ??? ??????????? ????????? > http://login.yahoo.com/config/mail?.intl=gr > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Melker.Goransson <@t> astrazeneca.com Wed Aug 20 03:57:29 2008 From: Melker.Goransson <@t> astrazeneca.com (=?iso-8859-1?Q?=22G=F6ransson=2C_Melker=22?=) Date: Wed Aug 20 03:57:41 2008 Subject: [Histonet] Adipose tissue processing Message-ID: <64A531C5BF0A1F4DA4D02849B43FEF370111FBA6@SEMLRDEMBX01.rd.astrazeneca.net> Hi all, I'm having trouble getting consistent good quality fixed sections from mouse retroperitoneal and epididymal fat. I need high quality morphology (intact cell membranes) in order to do computer based cell size measurements from 6?m sections. I fix 1x1 cm tissue in 10% formalin for 4 days, dehydrate to xylene and embed in paraffin. I get very variable result that I belive has to to with processing and not with the microtome cutting. I have tried sections from 4 to 12?m but thick sections tend to be even worse. Sometimes the sections are perfect and less than 1% off the cell membranes are broken, but sometimes the whole set of samples have more than 20% broken cell membranes. Does anyone had a similar experience or have any suggestions on how to best fix adipose tissue in order to get sections without broken cell membranes? I would be grateful for any help or suggestion. Thank you! > Melker G?ransson, PhD > From tere.insausti <@t> univ-tours.fr Wed Aug 20 08:11:24 2008 From: tere.insausti <@t> univ-tours.fr (Teresita Insausti) Date: Wed Aug 20 08:11:38 2008 Subject: [Histonet] electron microscopy for peroxidase activity Message-ID: <20080820131129.998D33C78054@rabelais.univ-tours.fr> Does anybody know the tetramethylbenzidine method for locating endogenous peroxidase activity using transmission electron microscopy? Could somebody help me, please? The protocol that I found says: ..."Transfer the tissue to freshly prepared 0.5 mg/ml tetramethylbenzidine in phosphate buffer (pH 4). After 3, 5, 7 hr add 0.01 % freshly prepared H2O2. Leave overnight and wash the tissue in cacodylate buffer"... 1) Is the indicated H2O2 concentration (0.01 %) the final one after adding it to the buffer containing the tetramethylbenzidine or that of the H2O2 solution to be added? 2) If so, what is the volume to be added? Thank you very much in advance Teresita Dr. Teresita Insausti Institut de Recherche sur la Biologie de l'Insecte (IRBI) Universit? Fran?ois Rabelais - Parc Grandmont 37200 Tours France >e-mail: tere.insausti@univ-tours.fr >Phone: +33 (0)2 47 36 73 66 >Fax: +33 (0)2 47 36 69 66 >http://www.univ-tours.fr/irbi/ From Heather.D.Renko <@t> osfhealthcare.org Wed Aug 20 08:17:16 2008 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Wed Aug 20 08:17:36 2008 Subject: [Histonet] re: cyrostat demo Message-ID: <40026EDDE64CDA47AB382C52619ACD3C0C44A04E@pmc-rfd-mx01.intranet.osfnet.org> Happy hump day everyone-we are halfway to the weekend! Fellow histotechs, I wanted input on the Microm cryostats of who likes the UV or the Vacutome/w chemical disinfection for an upcoming demo. Please reply directly to me for any feedback. Thank you in advance! Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From relia1 <@t> earthlink.net Wed Aug 20 08:53:41 2008 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Aug 20 08:53:48 2008 Subject: [Histonet] RELIA Special Job Alert !!! Supervisors and Managers Message-ID: Hi Histonetters!! I hope everyone is doing well. I have a few management positions that I want to let you know about. All of these are full time permanent positions. My clients are looking for candidates with a minimum of 3 years of histology and supervisory experience and ASCP certification. They are all offering competitive pay, benefits and relocation assistance. If you or someone you know might be interested please contact me. I can be reached at relia1@earthlink.net or toll free at 866-607-3542. Here is a list of the current supervisory and management opportunities I am recruiting for: Histology Manager - San Francisco, CA Histology Supervisor - Los Angeles Histology Manager - Buffalo, NY Histology Manager - Portland, OR Have a great day!! Thanks-Pam Thank You! Pam M. Barker President Relia 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll Free: (866)607-3542 e-mail: relia1@earthlink.net http://home.earthlink.net/~relia1 www.myspace.com/pamatrelia From cbass <@t> wfubmc.edu Wed Aug 20 08:55:45 2008 From: cbass <@t> wfubmc.edu (Caroline Bass) Date: Wed Aug 20 08:59:13 2008 Subject: [Histonet] Tubing for perfusion pump Message-ID: Hello, I?m hoping someone here can help me. I have a Manostat varistaltic pump, Vera model, that I would like to use for perfusing rats. It appears that the tubing currently in use is not the proper type, it is not very flexible and the motor is running too hard to drive the flow. I have been looking online to find either a manual for the pump or some recommendation for what kind of tubing to buy, but I haven?t had any luck. So two questions, first, does anyone have a manual or know what kind of tubing I should buy. Second, are there any recommendations for what to avoid with tubing? Thanks for your help, Caroline Bass From relia1 <@t> earthlink.net Wed Aug 20 09:21:47 2008 From: relia1 <@t> earthlink.net (Pam Barker) Date: Wed Aug 20 09:21:54 2008 Subject: [Histonet] RELIA Special Job Alert for Histotechnicians and Histotechnologists and one more supervisor job Message-ID: Hi Again Histonetters!! This one is for you technicinans and technologists. Just a few new positions I wanted to tell you about. All of these are full time permanent positions with excellent compensation benefits and relocation assistance. If you or someone you know might be interested please contact me at relia1@earthlink.net or toll free at 866-607-3542. New Grads are welcome to apply as well!! My clients are interested in people who are ASCP certified or eligible. Here are the new jobs I want to tell you about: Lab Supervisor - El Paso, TX Histo Tech - Baltimore, MD Histo Tech - El Paso, TX Histo Tech - Austin, TX Histo Tech - Seattle, WA Histo Tech - Spokane, WA Histo Tech - San Francisco Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.myspace.com/pamatrelia From RSRICHMOND <@t> aol.com Wed Aug 20 09:43:42 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Wed Aug 20 09:43:50 2008 Subject: [Histonet] Re: Looking for a Histobath Freezer Message-ID: Pat Patterson is >>looking for a Histobath Freezer that is in good operating condition. Please contact me directly at the email address given. Pat Patterson Supervisor, Immunohistochemistry pat.patterson@propath.com << This product was discontinued a couple of years ago, and I don't think they lasted very long, so it may be hard to find one. Loaded with acetone or 2-methylbutane, it was a real Molotov cocktail. If you get a used Histobath, contact me (or check the archives of this list) for a non-flammable freezing medium for it. Bob Richmond Samurai Pathologist Knoxville TN From rjbuesa <@t> yahoo.com Wed Aug 20 10:53:07 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Aug 20 10:53:15 2008 Subject: [Histonet] Microwave safety In-Reply-To: Message-ID: <610871.26684.qm@web65712.mail.ac4.yahoo.com> Joe: You are so RIGHT! Ren? J. --- On Tue, 8/19/08, Joe Nocito wrote: From: Joe Nocito Subject: Re: [Histonet] Microwave safety To: rjbuesa@yahoo.com, "Weems, Joyce" Cc: "histonet" Date: Tuesday, August 19, 2008, 5:33 PM this is my BIG beef. CAP insists on these questions, with the facade of safety concerns and runs with it. They're like law makers. They only make laws that doesn't affect them. My question is how many of the CAP has stock or some other private interest in these companies. Now for the good one (and I know I'm gonna get flamed on this one. This time, there is no CEO to call, so who has the last laugh now?) It's these microwave companies that lobby CAP, JACHO and OSHA in the "interest" of safety. Bull paddies!!! I've used household microwaves in the lab since the 1980's. Hell, Biogenex was touting antigen retrieval with lead thiocyanide using a microwave back in the late 80s and NOW, all of a sudden, it's a problem. Deer pebbles. My Knights of Columbus Council was having a taco sale at church one time. One of the members wrapped some tortillas in a towel to warm them up. He thought he set the timer for 2 minutes, but set it for 20 minutes. We went outside to sell our tacos. When we went back to the kitchen, the microwave was smoking and the towel was on fire. Almost set the church on fire. That's the only accident I had with a microwave. I've had more accidents using a hot plate than with a microwave. Here's where I date myself, I was heating up basic fuchsin making stock Mayer's mucicarmine solution. I walked away from the hot plate and forgot about it until I heard a POP. I ran back into the lab to find a nice magenta color all over the counter, ceiling and walls. The sad thing is that no one will confront CAP about this. I mean someone with clout. I may have a mouth, but I have no clout. A man has to know his limitations. We need to get together and petition CAP, but we won't get far because the pathologists own CAP. Now, let the flaming begin. JTT ----- Original Message ----- From: "Rene J Buesa" To: "Weems, Joyce" Cc: "histonet" Sent: Tuesday, August 19, 2008 10:28 AM Subject: RE: [Histonet] Microwave safety Yes, the train left the station long time ago, precisely the moment the CAP embraced this issue and decided to enforce it! Ren? J. --- On Tue, 8/19/08, Weems, Joyce wrote: From: Weems, Joyce Subject: RE: [Histonet] Microwave safety To: rjbuesa@yahoo.com Cc: "histonet" Date: Tuesday, August 19, 2008, 11:08 AM The sad thing is that we all know this, but it continues to be a growing issue in our country. What can we do to stop it? It's like a run-away train? j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, August 19, 2008 8:57 AM To: Joe Nocito; Anne van Binsbergen Cc: histonet Subject: Re: [Histonet] Microwave safety Joe and Anne: Have you both forgotten that every time a lab buys a cheap "home" microwave, the large lab equipment manufacturing companies lose a sale? Have you forgotten that if an accident happens (no matter how remote the possibility is) any "good claims attorney" will be able to twist the issue to "nail" somebody for using an equipment "against the manufacturer's specifications"? We are talking about monetary interests, not about personnel safety! Ren? J. --- On Tue, 8/19/08, Anne van Binsbergen wrote: From: Anne van Binsbergen Subject: Re: [Histonet] Microwave safety To: "Joe Nocito" Cc: "histonet" Date: Tuesday, August 19, 2008, 8:46 AM hey joe i have had accidents in my kitchen - but never in the lab im also getting a little tired of the CAP dictatorship - and we have not even started yet!!! if they were all dyed-in-the-wool histotechs with years and years of experience i would find it easier to accept - but then again if they had all that histo experience they would certainly not be expecting/demanding all these silly 'improvements' am also flipping annoyed that i am being told which EQA i can and cannot use - the list of CAP approved EQA facilities does not include NEQAS or RCPA QAP, both of which i use and am extremely satisfied with .... CAP are all about the money thats my vent for the decade whew....i feel better already!!!! abudhabiannie 2008/8/19 Joe Nocito > Histoland, > has anyone ever experienced or heard of an accident while using a "for home > use only" microwave? I have never had an accident. Before I go on another > tangent about CAP and OSHA, I'd like to know what everyone else experience > is. Since everyone is running around, changing the way they do things just > because CAP says breast cases have to be documented as to the length > of time > these cases have been fixing in formalin. Uh-oh, I feel it coming on. > Another tangent. I feel the flames warming up. > > JTT > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Aug 20 10:57:27 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Aug 20 10:57:35 2008 Subject: [Histonet] in situ question In-Reply-To: Message-ID: <65572.22025.qm@web65707.mail.ac4.yahoo.com> Exactly so! When you add water to the solid formaldehyde polymer?(=paraformaldehyde) you are just preparing a formaldehyde solution. Ren? J. --- On Tue, 8/19/08, Tony Henwood wrote: From: Tony Henwood Subject: RE: [Histonet] in situ question To: "N Fournier" , histonet@lists.utsouthwestern.edu Date: Tuesday, August 19, 2008, 8:01 PM Some questions, What is "PARA"? Is it an something you add to the formaldehyde solution? Please don't use the term 4% paraformaldehyde, use 4% formaldehyde, unless you are rolling the tissue in a powder consisting of 4% paraformaldehyde and 96% talcum powder or some other solid! Please refer to: Manoonkitiwongsa & Schultz (2002) Histochem J 34: 365-367 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of N Fournier Sent: Wednesday, 20 August 2008 3:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] in situ question Hi, I have a few questions regarding in situ hybridization. The protocol that I am following indicates that they perfused rats with PBS followed by 4% paraformaledhyde (in PBS). However, I always thought that one generally extracts the rat brain fresh, flash freeze, section, and then post-fix in 4% paraformaldehyde before commencing with the in situ hybridization. I suppose it might not matter since in the end the tissue is post-fixed in PARA; however, I would like to hear what others with experience doing in situ think about this procedure. Additionally, does one always use DEPC treated solutions even for these perfusion steps (e.g., PBS perfusion, paraformaldehyde perfusion)? Lastly, does one have to section on a cryostat? Could I simply use a vibrating microtome to section the tissue instead. Thanks for the help everyone, Neil _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From schaundrawalton <@t> yahoo.com Wed Aug 20 11:41:16 2008 From: schaundrawalton <@t> yahoo.com (Schaundra Walton) Date: Wed Aug 20 11:41:21 2008 Subject: [Histonet] Slide/Cassette Lablers Message-ID: <918647.93528.qm@web58906.mail.re1.yahoo.com> We are upgrading our LIS system and have the opportunity to possibly use some histology workflow solutions within the new system.? In light of this my boss has asked me to investigate block and slide lablers with the ability to print barcodes.? I have some info on the Shandon Microwriter Labeler series.? Has or is anyone using this?? What do you think?? Any suggestions for other lablers out there? ? Thanks, Schaundra Walton BS, HTL(ASCP) Histology Supervisor Swedish American Hospital Rockford, IL From LINDA.MARGRAF <@t> childrens.com Wed Aug 20 12:07:54 2008 From: LINDA.MARGRAF <@t> childrens.com (LINDA MARGRAF) Date: Wed Aug 20 12:09:43 2008 Subject: [Histonet] job listing Message-ID: <48AC0919.F783.00DA.0@childrens.com> Here's a job listing I was asked to post. Please respond to Nancy if you are interested. Thanks. Linda M Histonet administrator I am writing to enlist your help in identifying possible candidates for the Surgical Pathology Supervisor at one of our large teaching hospital clients. This unique career opportunity is available due to a recent reorganization. The Surgical Pathology Supervisor is responsible for the Histology Department that includes more than twenty-five FTE*s, and provides support to a world class pathology faculty acknowledged for their expertise and diagnostic capabilities. The Department processes more than 40,000 surgical pathology and ob/gyn cases/year, and performs more than 4,000 frozen sections/year. The Histology Department produces more than 200,000 blocks and 400,000 slides/year. Our client is committed to a state-of-the-art histology department, and is in the process of developing a plan to acquire *best of class* technology to meet that goal. Additionally, the Department has embarked on a path to reorganize its quality assurance plan to exceed the most rigorous inspection criteria. Hospital administration and pathology leadership strongly support both initiatives. The Hospital is located in New York City, and is recognized as one of the most prestigious health care institutions in the country. Qualified candidates for this exceptional career opportunity must possess a B.S. degree in Medical Technology or related science. HT or HTL (ASCP) certification required. Minimum of five years supervisory experience preferred, and at least six years experience as a Histotechnologist required. If you know an individual you believe may be interested in this opportunity, please call or email me. We would be happy to provide a position description, as well as discuss more information concerning our search. Thank you for your assistance. Health Care Development Services, Inc. Nancy Bielinski, Director LabLeadershipsm Executive Search PPhone: (847) 498-1122 Highland Park, IIL FFax: (847) 498-3264 Website: wwww.hcdsinc.com EEmail: NancyB@hcdsinc.com ( mailto:NancyB@hcdsinc.com ) Please consider the environment before printing this e-mail

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From kgustashaw <@t> aperio.com Wed Aug 20 12:43:01 2008 From: kgustashaw <@t> aperio.com (Karen Gustashaw) Date: Wed Aug 20 12:43:09 2008 Subject: [Histonet] Request for neuropathology slides Message-ID: <52F0A1B1F834C54DB1C0CF32182D6E9B0323CC54@sagan.aperio.int> Hi Everyone, Aperio is working on image analysis solutions for neuroscience applications. I'm interested in obtaining brain sections and/or spinal cord sections stained for any of the following, either with chromogens or fluorochromes: amyloid plaque Lewy bodies myelin In exchange, we will provide the whole-slide digital images, as well as an acknowledgment for any images that we use in our application notes (if desired). Please contact me off-line if you are interested in submitting slides. Thanks! Karen Karen M. Gustashaw, M.S. Product Manager, Fluorescence Aperio Technologies www.aperio.com kgustashaw@aperio.com (760) 304-6201 (voicemail) (703) 297-5107 (mobile) Aperio Science Seminar Series September 19: Boston, MA Pathology Visions 2008 October 26-28, 2008 San Diego, CA From McMahan <@t> vision.wustl.edu Wed Aug 20 13:59:01 2008 From: McMahan <@t> vision.wustl.edu (McMahan, Belinda) Date: Wed Aug 20 13:59:07 2008 Subject: [Histonet] Retriever 2100 Message-ID: <1C5A23F7B48786408C6636410C8675323A4DB1@EX04.wusm-pcf.wustl.edu> Good afternoon, Has anyone used the Retriever 2100 from EMS? It is a pressure cooker for antigen retrieval and I would like to know how it compares to the Decloaking Chamber from Biocare which is what I currently use. Any information will be helpful. Thanks, Belinda McMahan Washington University Dept. of Ophthalmology Immunomorphology Core Lab Campus Box 8096 St. Louis, MO 63110 314-362-3754 From DENNIST <@t> ascb.org Wed Aug 20 13:59:31 2008 From: DENNIST <@t> ascb.org (David Ennist) Date: Wed Aug 20 14:01:18 2008 Subject: [Histonet] brightfield images of mitochondria Message-ID: Dear Histonet Members, I work at the American Society for Cell Biology developing a free Internet resource for cell biology, the ASCB Image & Video Library (http://cellimages.ascb.org) that is aimed at the undergraduate to graduate student level. The ASCB Council has asked that I work my way through cell biology, subject by subject, finding and annotating images (LM, EM, fluorescence), diagrams and videos that illustrate all aspects of cell biology. I recently worked on the centrosome (http://tinyurl.com/62uyxj) and am now moving on to mitochondria. In this regard, I would like to invite you to submit any brightfield images of mitochondria that you may have (eg, stained by the method of Cain, etc). You can submit them online at: http://www.ascb.org/ivl/ . Once you establish an account, you can choose a simple figure legend annotation for Research (option C) or fill out the annotation developed by the ASCB Education Committee (option B). Of course, any other images relevant to cell biology will be most welcome and will be considered for inclusion in the Image & Video Library! Regards, Dave David L. Ennist, PhD Director, Digital Resources The American Society for Cell Biology 8120 Woodmont Avenue, Suite 750 Bethesda, MD 20814-2762 TEL: 301-347-9315 FAX: 301-347-9310 email: DEnnist@ascb.org website: http://cellimages.ascb.org/ See you at the 48th ASCB Annual Meeting! December 13-17, 2008 in San Francisco, CA. For more information, go to www.ascb.org Sign up for the Wikipedia Workshop: https://www.ascb.org/ascbsec/int_workshop1.cfm Enter the Celldance Contest: http://ascb.org/index.cfm?navid=139 From Norm.Burnham <@t> propath.com Wed Aug 20 15:55:43 2008 From: Norm.Burnham <@t> propath.com (Norm Burnham) Date: Wed Aug 20 15:56:01 2008 Subject: [Histonet] Histology Manager needed for growing Pathology company Message-ID: <82C7248978CB50469FD6BA68EBBEFE67347934@exchange.propathlab.com> Histology Manager ProPath, a large and growing and progressive, CAP accredited, pathology practice in Dallas, TX is conducting a search for an experienced team-oriented leader to manage all Histology operations, e.g., a staff of over 25 FTE's, recruiting, hiring, training and scheduling staff; supervising histology operation to ensure quality of slides and that department goals are met; maintaining preparedness for all accrediting/regulatory agency inspections, and general troubleshooting. The organization's pathologists and staff are committed to providing unsurpassed pathology services and we utilize leading technology. Competitive salary with a sign-on bonus and relocation assistance offered. Medical, dental, company paid STD and LTD insurance, a matched 401K plan and more. Qualified candidates for this outstanding career opportunity must possess HT/HTL (ASCP) certification, a minimum of 8 years histology experience including a minimum of 5 years supervisory experience. The minimal education requirement is an Associate Degree in a related field but a Bachelor Degree in Science is preferred. This newly-created position is available due to expansion of clients nationwide. ProPath is located in Dallas, Texas where housing costs are reasonable and there is no state income tax. If you know an individual you believe may be interested in this exceptional opportunity, please call or email: ProPath Human Resources 8267 Elmbrook, Suite 100, Dallas, TX 75247. FAX: 214/237-1825. Job-Line 214/237-1775. Email address. jobs@propathlab.com Website www.propathlab.com EOE. ______________________________________________________________________________ This e-mail may contain confidential or privileged information. If you think you have received this e-mail in error, please advise the sender by reply e-mail and then delete this e-mail immediately. From Norm.Burnham <@t> propath.com Wed Aug 20 15:58:27 2008 From: Norm.Burnham <@t> propath.com (Norm Burnham) Date: Wed Aug 20 15:58:33 2008 Subject: [Histonet] Quality Assurance Coordinator needed for growing Pathology practice Message-ID: <82C7248978CB50469FD6BA68EBBEFE6734793C@exchange.propathlab.com> Quality Assurance Coordinator ProPath, a large and growing, highly-respected, and progressive, CAP accredited, pathology practice in Dallas, TX is conducting a search for an experienced team-oriented Quality Assurance Coordinator to manage our Quality Improvement and Peer Review programs, and over-site of our CAP/CLIA/state licensure inspection readiness. The organization's world class pathologists and staff are committed to providing the highest quality and state-of-the-art anatomic and clinical pathology services. Competitive salary and relocation assistance offered. Medical, dental, company paid STD and LTD insurance, a matched 401K plan and more Qualified candidates for this outstanding career opportunity must possess a minimum of 5 years experience working in a health-related position with solid knowledge of process and quality improvement, QA/Peer Review and CAP. Bachelors degree preferred (bachelor of science is desired); lack of degree may be offset by significant experience in related field, in analyzing and improving processes, and in working with high levels of management to identify and control risk. Certification as MT, CT, or HT/HTL is desirable ProPath is located in Dallas, Texas where housing costs are reasonable and there is no state income tax. If you know an individual you believe may be interested in this exceptional opportunity, please contact: ProPath Human Resources 8267 Elmbrook, Suite 100, Dallas, TX 75247. FAX: 214/237-1825. Job-Line 214/237-1775. Email address. jobs@propathlab.com Website www.propathlab.com EOE. ______________________________________________________________________________ This e-mail may contain confidential or privileged information. If you think you have received this e-mail in error, please advise the sender by reply e-mail and then delete this e-mail immediately. From Godsgalnow <@t> aol.com Wed Aug 20 16:52:39 2008 From: Godsgalnow <@t> aol.com (Godsgalnow@aol.com) Date: Wed Aug 20 16:52:45 2008 Subject: [Histonet] proficiency test regs Message-ID: I am calling on the experts...... For required Proficiency testing (say HPV via PCR) do you have to have a proficiency test for each different type of sample (say biopsy and pap) and collection method (say Surepath and another type of fixative) you do? I guess what I am getting at is must you enroll in PT for each fixative for a required test? Roxanne **************It's only a deal if it's where you want to go. Find your travel deal here. (http://information.travel.aol.com/deals?ncid=aoltrv00050000000047) From Godsgalnow <@t> aol.com Wed Aug 20 17:48:48 2008 From: Godsgalnow <@t> aol.com (Godsgalnow@aol.com) Date: Wed Aug 20 17:48:58 2008 Subject: [Histonet] pt providers Message-ID: Anyone have the list of CAP approved proficiency testing providers? I am having a hard time finding it on their website. Thanks, Roxanne **************It's only a deal if it's where you want to go. Find your travel deal here. (http://information.travel.aol.com/deals?ncid=aoltrv00050000000047) From AnthonyH <@t> chw.edu.au Wed Aug 20 18:00:16 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Aug 20 18:00:28 2008 Subject: FW: [Histonet] in situ question Message-ID: Ruth, But Paraformaldehyde is the powder. When you chemically treat it (sodium Hydroxide & heat) it dissolves and forms formaldehyde. So the correct term is 4% buffered formaldehyde or if made from concentrated formalin (the liquid form) it is termed 10%buffered formalin (c. 4% buffered formaldehyde). Paraformaldehyde is poorly soluble in pH neutral water. It's the terminology that many of us often have issue with, apart from problems of short fixation and why bother to use paraformaldehyde in the first place when formalin is safer and does an equivalent if not better job! Please refer to the article: Manoonkitiwongsa & Schultz (2002) Histochem J 34: 365-367 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 -----Original Message----- From: Yaskovich, Ruth A (NIH/NIDCR) [E] [mailto:ryaskovich@dir.nidcr.nih.gov] Sent: Thursday, 21 August 2008 12:15 AM To: Tony Henwood Subject: RE: [Histonet] in situ question Tony, It's actually a 4% BUFFERED Parformaldehyde. It works a lot better with a buffer. Ruth N.I.H. -----Original Message----- From: Tony Henwood [mailto:AnthonyH@chw.edu.au] Sent: Tuesday, August 19, 2008 8:02 PM To: N Fournier; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] in situ question Some questions, What is "PARA"? Is it an something you add to the formaldehyde solution? Please don't use the term 4% paraformaldehyde, use 4% formaldehyde, unless you are rolling the tissue in a powder consisting of 4% paraformaldehyde and 96% talcum powder or some other solid! Please refer to: Manoonkitiwongsa & Schultz (2002) Histochem J 34: 365-367 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of N Fournier Sent: Wednesday, 20 August 2008 3:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] in situ question Hi, I have a few questions regarding in situ hybridization. The protocol that I am following indicates that they perfused rats with PBS followed by 4% paraformaledhyde (in PBS). However, I always thought that one generally extracts the rat brain fresh, flash freeze, section, and then post-fix in 4% paraformaldehyde before commencing with the in situ hybridization. I suppose it might not matter since in the end the tissue is post-fixed in PARA; however, I would like to hear what others with experience doing in situ think about this procedure. Additionally, does one always use DEPC treated solutions even for these perfusion steps (e.g., PBS perfusion, paraformaldehyde perfusion)? Lastly, does one have to section on a cryostat? Could I simply use a vibrating microtome to section the tissue instead. Thanks for the help everyone, Neil _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From annigyg <@t> gmail.com Wed Aug 20 23:10:31 2008 From: annigyg <@t> gmail.com (Anne van Binsbergen) Date: Wed Aug 20 23:10:38 2008 Subject: [Histonet] Microwave safety In-Reply-To: <610871.26684.qm@web65712.mail.ac4.yahoo.com> References: <610871.26684.qm@web65712.mail.ac4.yahoo.com> Message-ID: well said Joe - my thoughts exactly - money talks Annie 2008/8/20 Rene J Buesa > Joe: > You are so RIGHT! > Ren? J. > > --- On Tue, 8/19/08, Joe Nocito wrote: > > From: Joe Nocito > Subject: Re: [Histonet] Microwave safety > To: rjbuesa@yahoo.com, "Weems, Joyce" > Cc: "histonet" > Date: Tuesday, August 19, 2008, 5:33 PM > > this is my BIG beef. CAP insists on these questions, with the facade of > safety concerns and runs with it. They're like law makers. They only make > laws that doesn't affect them. My question is how many of the CAP has stock > > or some other private interest in these companies. Now for the good one > (and > I know I'm gonna get flamed on this one. This time, there is no CEO to > call, > so who has the last laugh now?) It's these microwave companies that lobby > CAP, JACHO and OSHA in the "interest" of safety. Bull paddies!!! > I've used > household microwaves in the lab since the 1980's. Hell, Biogenex was > touting > antigen retrieval with lead thiocyanide using a microwave back in the late > 80s and NOW, all of a sudden, it's a problem. Deer pebbles. > My Knights of Columbus Council was having a taco sale at church one time. > One of the members wrapped some tortillas in a towel to warm them up. He > thought he set the timer for 2 minutes, but set it for 20 minutes. We went > outside to sell our tacos. When we went back to the kitchen, the microwave > was smoking and the towel was on fire. Almost set the church on fire. > That's > the only accident I had with a microwave. I've had more accidents using a > hot plate than with a microwave. Here's where I date myself, I was heating > > up basic fuchsin making stock Mayer's mucicarmine solution. I walked away > from the hot plate and forgot about it until I heard a POP. I ran back into > the lab to find a nice magenta color all over the counter, ceiling and > walls. > The sad thing is that no one will confront CAP about this. I mean someone > with clout. I may have a mouth, but I have no clout. A man has to know his > limitations. We need to get together and petition CAP, but we won't get far > > because the pathologists own CAP. > Now, let the flaming begin. > > JTT > ----- Original Message ----- > From: "Rene J Buesa" > To: "Weems, Joyce" > Cc: "histonet" > Sent: Tuesday, August 19, 2008 10:28 AM > Subject: RE: [Histonet] Microwave safety > > > Yes, the train left the station long time ago, precisely the moment the CAP > embraced this issue and decided to enforce it! > Ren? J. > > --- On Tue, 8/19/08, Weems, Joyce wrote: > > From: Weems, Joyce > Subject: RE: [Histonet] Microwave safety > To: rjbuesa@yahoo.com > Cc: "histonet" > Date: Tuesday, August 19, 2008, 11:08 AM > > The sad thing is that we all know this, but it continues to be a growing > issue > in our country. What can we do to stop it? It's like a run-away train? j > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J > Buesa > Sent: Tuesday, August 19, 2008 8:57 AM > To: Joe Nocito; Anne van Binsbergen > Cc: histonet > Subject: Re: [Histonet] Microwave safety > > Joe and Anne: > Have you both forgotten that every time a lab buys a cheap "home" > microwave, the large lab equipment manufacturing companies lose a sale? > Have you forgotten that if an accident happens (no matter how remote the > possibility is) any "good claims attorney" will be able to twist the > issue to "nail" somebody for using an equipment "against the > manufacturer's specifications"? > We are talking about monetary interests, not about personnel safety! > Ren? J. > > --- On Tue, 8/19/08, Anne van Binsbergen wrote: > > From: Anne van Binsbergen > Subject: Re: [Histonet] Microwave safety > To: "Joe Nocito" > Cc: "histonet" > Date: Tuesday, August 19, 2008, 8:46 AM > > hey joe > i have had accidents in my kitchen - but never in the lab > > im also getting a little tired of the CAP dictatorship - and we have not > even > started yet!!! > if they were all dyed-in-the-wool histotechs with years and years of > experience > i would find it easier to accept - but then again if they had all that > histo > experience they would certainly not be expecting/demanding all these silly > 'improvements' > > am also flipping annoyed that i am being told which EQA i can and cannot > use > - the list of CAP approved EQA facilities does not include NEQAS or RCPA > QAP, > both of which i use and am extremely satisfied with .... > > CAP are all about the money > > thats my vent for the decade > > whew....i feel better already!!!! > abudhabiannie > 2008/8/19 Joe Nocito > > > Histoland, > > has anyone ever experienced or heard of an accident while using a > "for home > > use only" microwave? I have never had an accident. Before I go on > another > > tangent about CAP and OSHA, I'd like to know what everyone else > experience > > is. Since everyone is running around, changing the way they do things > just > > because CAP says breast cases have to be documented as to the length > > of > time > > these cases have been fixing in formalin. Uh-oh, I feel it coming on. > > Another tangent. I feel the flames warming up. > > > > JTT > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Anne van Binsbergen (Hope) > Abu Dhabi > UAE > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This email, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please reply to the > sender that you have received the message in > error, then delete this message. > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Anne van Binsbergen (Hope) Abu Dhabi UAE From cbass <@t> wfubmc.edu Thu Aug 21 00:03:48 2008 From: cbass <@t> wfubmc.edu (Caroline Bass) Date: Thu Aug 21 00:03:59 2008 Subject: [Histonet] Peristaltic pump tubing In-Reply-To: Message-ID: Hello, I?m hoping someone here can help me. I have a Manostat varistaltic pump, Vera model, that I would like to use for perfusing rats. It appears that the tubing currently in use is not the proper type, it is not very flexible and the motor is running too hard to drive the flow. I have been looking online to find either a manual for the pump or some recommendation for what kind of tubing to buy, but I haven?t had any luck. So two questions, first, does anyone have a manual or know what kind of tubing I should buy. Second, are there any recommendations for what to avoid with tubing? Please forgive if this is a double post, I?ve been having email issues today, and I didn?t see this email on my daily digest. Thanks for your help, Caroline Bass From zumbor <@t> email.cs.nsw.gov.au Thu Aug 21 02:56:02 2008 From: zumbor <@t> email.cs.nsw.gov.au (Rosalba Zumbo) Date: Thu Aug 21 02:54:35 2008 Subject: [Histonet] Repair freezing artifact in muscle biopsies. Message-ID: <000001c90363$5b5da840$1218f8c0$@cs.nsw.gov.au> Hi All, I am interested in finding out how to repair a muscle biopsy that has freezing artifact. I saw on the Histologic website there is a reference to an article on how to do it. Unfortunately that seems to be the only issue I cannot open. Could someone please share their knowledge on how to fix freezing artifact or a copy of May 2003 Histologic. Thanks Rosalba Zumbo Supervisor Histology Dept Department of Forensic Medicine 42-50 Parramatt Rd Glebe NSW 2041 Australia Ph: 61 2 85847842 Fax: 61 2 95664573 zumbor@email.cs.nsw.gov.au From mikael.niku <@t> helsinki.fi Thu Aug 21 03:49:55 2008 From: mikael.niku <@t> helsinki.fi (Mikael Niku) Date: Thu Aug 21 03:50:02 2008 Subject: [Histonet] (para)formaldehyde (was: in situ question) In-Reply-To: References: <001301c90261$093fcff0$be00000a@CPU360> Message-ID: <006e01c9036a$e2abb3d0$97a5d680@mmkem12636> This has probably been discussed 1000s of times, but.... I'm wondering to what extent dissolved paraformaldehyde really is equivalent to 4% formaldehyde or 10% formalin. In my experience, samples fixed in formalin and "4% PFA" as we tend to say are different when one does immunostaining or especially in situ hybridization. Formalin is of course traditionally used in pathology, whereas many labs doing immunos or ISH use "PFA". But what really causes the differences? Is it incomplete de-polymerization of paraformaldehyde, or perhaps the methanol typically included in formalin? ----------------------------------------------------- Mikael Niku, PhD, university lecturer University of Helsinki, Division of Nutrition URL: mikael.nikunnakki.info - What do I think of western civilization? I think it would be a good idea! Gandhi ----------------------------------------------------- -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: 20. elokuuta 2008 4:42 To: Lee Crosby; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] in situ question Which the becomes 4% formaldehyde or equivalent to 10% formalin. Not 4% paraformaldehyde. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 From Barry.R.Rittman <@t> uth.tmc.edu Thu Aug 21 06:15:47 2008 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Thu Aug 21 06:15:51 2008 Subject: [Histonet] Peristaltic pump tubing References: Message-ID: Caroline it is over 20 years since we used a peristaltic pump but... We preferred using a medium thickness walled latex rather than plastic tubing. Have to have a balance between pump being able to compress the tubing, ability of tubing not to kink and also exit catheter resistance. I would recommend that you contact the company directly, they are usually really helpful and will have some suggestions on this and may give you some specifications. Hope that this helps Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Caroline Bass Sent: Thu 8/21/2008 12:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Peristaltic pump tubing Hello, I?m hoping someone here can help me. I have a Manostat varistaltic pump, Vera model, that I would like to use for perfusing rats. It appears that the tubing currently in use is not the proper type, it is not very flexible and the motor is running too hard to drive the flow. I have been looking online to find either a manual for the pump or some recommendation for what kind of tubing to buy, but I haven?t had any luck. So two questions, first, does anyone have a manual or know what kind of tubing I should buy. Second, are there any recommendations for what to avoid with tubing? Please forgive if this is a double post, I?ve been having email issues today, and I didn?t see this email on my daily digest. Thanks for your help, Caroline Bass _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> heart.thi.tmc.edu Thu Aug 21 06:43:53 2008 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Thu Aug 21 06:44:01 2008 Subject: [Histonet] fibrin gel to paraffin Message-ID: Hi, I have some "outgrowths" that are in a modified fibrin gel. They vary in size from.1 to 1mm thick. The gel is porous and optically clear for the most part. They have been fixed in 4% paraformaldehyde. They were done in well plates or chamber slides. The researchers want them processed for paraffin. I have no experience with this technique and would be grateful for any suggestions. Thank you. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. MC 1-283 Houston, TX 77030 832-355-6524 832-355-6812 (fax) From petepath <@t> yahoo.com Thu Aug 21 06:54:53 2008 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Thu Aug 21 06:55:00 2008 Subject: [Histonet] Histotechnologist Opening Northern New Jersey Message-ID: <78604.39260.qm@web45105.mail.sp1.yahoo.com> We have an full time opening for a histotechnologist at Hackensack University Medical Center. Our ideal candidate will have immunohistochemistry experience as well. Contact Jackie Brown at jbrown@humed.com Stephen Peters M.D. Vice Chairman?of Pathology Hackensack University Medical Center ? ? From CKelley <@t> bostwicklaboratories.com Thu Aug 21 07:48:23 2008 From: CKelley <@t> bostwicklaboratories.com (Chester Kelley) Date: Thu Aug 21 07:48:30 2008 Subject: [Histonet] Job Opportunity - Histotechnologist Message-ID: <24D22DE9E488AA43BF92A4389F2DDB1F036A961D@mail1.BOSTWICK.COM> Good Morning I would like to post the following on the Histonet list: About us - Bostwick Laboratories(r) is a full-service laboratory specializing in uropathology, dedicated to the diagnosis, treatment and subsequent management of prostate cancer, kidney disease, cancer of the bladder and other urologic conditions. For more information, visit us on the web at www.bostwicklaboratories.com. We are currently seeking the following to join our growing team: Histotechnologist - VA, TN & NY This position will be responsible for all elements of biopsy processing and staining for diagnosis. Essential duties include: -Ensure all laboratory specimens are accurately and efficiently handled -Processing, embedding, microtomy, staining, coverslipping, and quality control of histology specimens -Ensure safety and HIPAA regulations are observed at all times -Participate in mentoring, ongoing education/training, and workflow analysis -Other duties and projects as needed Benefits - -Medical, dental, and vision insurance -Company paid life and long-term disability insurance -Vacation accrual (10 days during the first year, 15 days after one full year of employment) -Sick time and personal leave accruals -Healthcare and dependent care flexible spending accounts Requirements - -Bachelor's degree in Histology or related science from an accredited school preferred -Training and certification as HT or HTL by ASCP -Excellent communication skills -Flexible and a team player -Pre-employment background check required Please let me know the next steps in the posting process. Thanks Chester Kelley Sr. Human Resources Generalist Bostwick Laboratories(r) For Absolute Confidence(r) 4355 Innslake Drive Glen Allen, Virginia 23060 Phone: (804) 967-9225 x2129 Email: CKelley@bostwicklaboratories.com From rjbuesa <@t> yahoo.com Thu Aug 21 07:54:05 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Aug 21 07:54:12 2008 Subject: [Histonet] (para)formaldehyde (was: in situ question) In-Reply-To: <006e01c9036a$e2abb3d0$97a5d680@mmkem12636> Message-ID: <477868.38916.qm@web65708.mail.ac4.yahoo.com> Paraformaldehyde is the 80 to 100 units polymer of methanal (formaldehyde) and is called polyoxymethylene. When hydrated it will render a?pure? formaldehyde (methanal) solution to the concentration you desire. ? Formalin or formol, depending on the commercial name used to designate it,?is the solution of methanal (formaldehyde) obtained by catalytic oxidation of methanol and bubbled through water to a concentration of 37-50% w/w to which 7-15% methanol is added as a stabilizer to retard its polymerization. ? Formaldehyde is so reactive with water that it is present in the formalin as a concentration of? 0.1% only, the rest (99.9%) is present as methanediol or methylene glycol. From this last chemical the formaldehyde has to be reconstituted during fixation, hence the slow fixation rate of formalin but its rapid penetration rate (penetration about 48 times faster than fixation). ? In the same way as formaldehyde bubbled through water reacts and forms methylene glycol, the same reaction will take place when disolving paraformaldehyde. ? Both solutions are "formaldehyde solutions" the only thing is that formalin contains methanol as a stabilizer and the formaldehyde solution from paraformaldehyde does not, unless you decide to add it. Ren? J.? --- On Thu, 8/21/08, Mikael Niku wrote: From: Mikael Niku Subject: RE: [Histonet] (para)formaldehyde (was: in situ question) To: histonet@lists.utsouthwestern.edu Date: Thursday, August 21, 2008, 4:49 AM This has probably been discussed 1000s of times, but.... I'm wondering to what extent dissolved paraformaldehyde really is equivalent to 4% formaldehyde or 10% formalin. In my experience, samples fixed in formalin and "4% PFA" as we tend to say are different when one does immunostaining or especially in situ hybridization. Formalin is of course traditionally used in pathology, whereas many labs doing immunos or ISH use "PFA". But what really causes the differences? Is it incomplete de-polymerization of paraformaldehyde, or perhaps the methanol typically included in formalin? ----------------------------------------------------- Mikael Niku, PhD, university lecturer University of Helsinki, Division of Nutrition URL: mikael.nikunnakki.info - What do I think of western civilization? I think it would be a good idea! Gandhi ----------------------------------------------------- -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: 20. elokuuta 2008 4:42 To: Lee Crosby; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] in situ question Which the becomes 4% formaldehyde or equivalent to 10% formalin. Not 4% paraformaldehyde. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Thu Aug 21 08:40:46 2008 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Aug 21 08:40:19 2008 Subject: [Histonet] (para)formaldehyde In-Reply-To: <006e01c9036a$e2abb3d0$97a5d680@mmkem12636> References: <001301c90261$093fcff0$be00000a@CPU360> <006e01c9036a$e2abb3d0$97a5d680@mmkem12636> Message-ID: <48AD705E.4000706@umdnj.edu> Greetings Mikael: What differences are you talking about? What leads you to suspect that one fixative is different from the other? And are all of the other variables controlled? Fixation time, temperature, method of application of the fixative, size of the tissue, etc.The amount of methanol in a "10% formalin" solution is 1.2-1.5% versus zero for fix made from paraformaldehyde. Geoff Mikael Niku wrote: > This has probably been discussed 1000s of times, but.... > > I'm wondering to what extent dissolved paraformaldehyde really is equivalent > to 4% formaldehyde or 10% formalin. > In my experience, samples fixed in formalin and "4% PFA" as we tend to say > are different when one does immunostaining or especially in situ > hybridization. Formalin is of course traditionally used in pathology, > whereas many labs doing immunos or ISH use "PFA". > > But what really causes the differences? Is it incomplete de-polymerization > of paraformaldehyde, or perhaps the methanol typically included in formalin? > > ----------------------------------------------------- > Mikael Niku, PhD, university lecturer > University of Helsinki, Division of Nutrition > URL: mikael.nikunnakki.info > > - What do I think of western civilization? > I think it would be a good idea! > Gandhi > ----------------------------------------------------- > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood > Sent: 20. elokuuta 2008 4:42 > To: Lee Crosby; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] in situ question > > Which the becomes 4% formaldehyde or equivalent to 10% formalin. Not 4% > paraformaldehyde. > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & > Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, > Westmead NSW 2145 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From JMacDonald <@t> mtsac.edu Thu Aug 21 09:06:14 2008 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Aug 21 09:06:08 2008 Subject: [Histonet] Shandon Varistain XY instruction manual Message-ID: I am trying to locate an instruction manual for a Shandon, Varistain XY autostainer. Can anyone help out? Thank you, Jennifer Jennifer MacDonald Director, Histotechnician Training Program Mt. San Antonio College 1100 N. Grand Ave. Walnut, CA 91789 (909) 594-5611 ext. 4884 jmacdonald@mtsac.edu From kadamsplw <@t> gmail.com Thu Aug 21 09:38:41 2008 From: kadamsplw <@t> gmail.com (karen adams) Date: Thu Aug 21 09:38:50 2008 Subject: [Histonet] polysciences Message-ID: Can someone give me the name of a rep. for polysciences, Inc.....I have an issue I would like to discuss. Thanks, -- Karen Adams Pathology Laboratories West 9303 Park West Blvd. Knoxville, TN 37923 kadamsplw@gmail.com From pruegg <@t> ihctech.net Thu Aug 21 10:09:02 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu Aug 21 10:09:18 2008 Subject: [Histonet] fibrin gel to paraffin In-Reply-To: References: Message-ID: <775D62DCD949415883F4A365127C9B07@ihctechq9h2qof> Betsy, I would wrap them in paper (perm curl paper) and process as usual on a tissue processor, I use a shorter than usual schedule for animal tissue and things like this, 20 min in each change of alcohols, xylene and paraffins. Embed on edge to get a cross section of all the layers. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Thursday, August 21, 2008 5:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fibrin gel to paraffin Hi, I have some "outgrowths" that are in a modified fibrin gel. They vary in size from.1 to 1mm thick. The gel is porous and optically clear for the most part. They have been fixed in 4% paraformaldehyde. They were done in well plates or chamber slides. The researchers want them processed for paraffin. I have no experience with this technique and would be grateful for any suggestions. Thank you. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. MC 1-283 Houston, TX 77030 832-355-6524 832-355-6812 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AFoshey <@t> chw.org Thu Aug 21 12:09:33 2008 From: AFoshey <@t> chw.org (Foshey, Annette) Date: Thu Aug 21 12:09:37 2008 Subject: [Histonet] Proficiency testing for immunostaining Message-ID: We are starting to do Immunostaining but do not do HER 2 testing what type of PT is recommended or required? I appreciate any input received. Thanks, Annette Foshey, HT (ASCP) Team Leader in Histology Children's Hospital of Wisconsin 414-266-6580 Fax 414-266-2779 afoshey@chw.org ************************** Children's Hospital and Health System recognizes that unencrypted e-mail is not secure and does not guarantee confidentiality. The confidentiality of replies to this message cannot be guaranteed unless the replies are encrypted. From bakevictoria <@t> gmail.com Thu Aug 21 12:30:43 2008 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Thu Aug 21 12:30:48 2008 Subject: [Histonet] polysciences In-Reply-To: References: Message-ID: <4f016b690808211030sc1db8c8i1c6ec03ef0dd1c00@mail.gmail.com> 1-800-523-2575 Ask for Dana Dittus. On Thu, Aug 21, 2008 at 10:38 AM, karen adams wrote: > Can someone give me the name of a rep. for polysciences, Inc.....I have an > issue I would like to discuss. > > Thanks, > > -- > Karen Adams > Pathology Laboratories West > 9303 Park West Blvd. > Knoxville, TN 37923 > kadamsplw@gmail.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Jackie.O'Connor <@t> abbott.com Thu Aug 21 14:25:10 2008 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Aug 21 14:25:33 2008 Subject: [Histonet] Electronic tracking for pharma histo labs In-Reply-To: <82C7248978CB50469FD6BA68EBBEFE67347934@exchange.propathlab.com> Message-ID: I'm curious as to what electronic histology tracking systems are in use by other pharma histo labs. Do you use in-house developed software, or has a vendor provided a histology module for you. Do you still use a paper trail to document histology samples, blocks, slides, archiving? Please advise. Thanks, Jackie O'Connor From gentras <@t> vetmed.auburn.edu Thu Aug 21 14:50:24 2008 From: gentras <@t> vetmed.auburn.edu (Atoska Gentry) Date: Thu Aug 21 14:50:53 2008 Subject: [Histonet] RE: IHC in Cats Message-ID: <48ADC700.2070302@vetmed.auburn.edu> Hello, is anyone having success with IHC in cats for the following ab's: CD4, CD8, and/or MHC2? If so will you please share your protocol (s) , ab source and any other pertinent info with me ASAP? Thanks, Atoska From sbreeden <@t> nmda.nmsu.edu Thu Aug 21 14:57:59 2008 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Aug 21 14:58:09 2008 Subject: [Histonet] Position Posting Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E647E@nmdamailsvr.nmda.ad.nmsu.edu> I am posting this on another's behalf: a laboratory equipment repair company that I work with is looking for service technicians. The candidate will be a person who is already familiar with medical laboratory equipment and a strong electronics background. If there is such an individual that is interested in making a change from laboratory to service on equipment on which they would already be familiar, this is a good opportunity. The company will train a qualified individual. If you are interested, send an email to dkmg4@aol.com or contact me directly. Friday is right around the corner and I'm trolling for a Flaming Friday Subject. Anyone? I can also gripe on behalf of others (for a small fee)! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From trathborne <@t> somerset-healthcare.com Thu Aug 21 15:03:42 2008 From: trathborne <@t> somerset-healthcare.com (Rathborne, Toni) Date: Thu Aug 21 15:03:51 2008 Subject: [Histonet] Flaming Friday Subject In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B017E647E@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: How about demo units that are non-functioning? CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. From JWeems <@t> sjha.org Thu Aug 21 15:11:35 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Thu Aug 21 15:11:41 2008 Subject: [Histonet] Flaming Friday Subject In-Reply-To: References: <4D14F0FC9316DD41972D5F03C070908B017E647E@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <982A0A9461F9BF438C7B19A6E425A3835170C9@ITSSSXM01V6.one.ads.che.org> Oh.. That's good - get us started! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Thursday, August 21, 2008 4:04 PM To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: [Histonet] Flaming Friday Subject How about demo units that are non-functioning? CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From mary.gessford <@t> spcorp.com Thu Aug 21 15:18:03 2008 From: mary.gessford <@t> spcorp.com (Gessford, Mary) Date: Thu Aug 21 15:18:08 2008 Subject: [Histonet] Electronic tracking for pharma histo labs In-Reply-To: Message-ID: Path Data Systems is the most popular at this time. Xybion 422 was the most popular in the past. Most of the big Pharma labs are going with PDS. Mary Gessford Supervisor Anatomic Pathology Schering-Plough 908-473-4358 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Thursday, August 21, 2008 3:25 PM To: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: [Histonet] Electronic tracking for pharma histo labs I'm curious as to what electronic histology tracking systems are in use by other pharma histo labs. Do you use in-house developed software, or has a vendor provided a histology module for you. Do you still use a paper trail to document histology samples, blocks, slides, archiving? Please advise. Thanks, Jackie O'Connor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From rjbuesa <@t> yahoo.com Thu Aug 21 15:27:25 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Aug 21 15:27:28 2008 Subject: [Histonet] Flaming Friday Subject In-Reply-To: Message-ID: <236614.62990.qm@web65715.mail.ac4.yahoo.com> Don't even think in buying it or "a like substitute" Ren? J. --- On Thu, 8/21/08, Rathborne, Toni wrote: From: Rathborne, Toni Subject: [Histonet] Flaming Friday Subject To: "Breeden, Sara" , histonet@lists.utsouthwestern.edu Date: Thursday, August 21, 2008, 4:03 PM How about demo units that are non-functioning? CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From will <@t> histologytechservices.com Thu Aug 21 16:13:35 2008 From: will <@t> histologytechservices.com (William Connor) Date: Thu Aug 21 16:13:46 2008 Subject: [Histonet] Hyaluronidase Message-ID: <0MKp8S-1KWHTP2zxq-0003wW@mrelay.perfora.net> Greetings, How interchangeable are hyaluronidase and Proteinase K for an IHC procedure? If the protocol calls for hyaluronidase, had I better use it specifically? William P. Connor, HT(ASCP) Senior Histology Technician Histology Tech Services, Inc. This email and its attachments (if any) contain confidential and privileged information. Any dissemination or use of this information by a person other than the intended recipient is unauthorized and may be illegal. From amylee779 <@t> yahoo.com Thu Aug 21 17:22:46 2008 From: amylee779 <@t> yahoo.com (Amy Lee) Date: Thu Aug 21 17:22:49 2008 Subject: [Histonet] counterstain on bloodsmear after DAB Message-ID: <425949.45524.qm@web38002.mail.mud.yahoo.com> Hello, Could anybody tell me what kind of counterstain you use for blood smear after DAB? I used to use hemotoxyline on tissues but this time it's blood smear. ? Any advice is highly appreciated! ? Amy From rosenfeldtek <@t> hotmail.com Thu Aug 21 17:40:27 2008 From: rosenfeldtek <@t> hotmail.com (JR R) Date: Thu Aug 21 17:40:32 2008 Subject: [Histonet] This Hematoxylin Shortage is starting to really hurt! Message-ID: I ordered Hematoxylin powder fromg Sogma *Catalog H-3136) In Early April--they said it was backordered. I still haven't received my powder, and I'm about to run out. I am doing large volume Movat Pentachrome Staining and the Hematoxylin substitutes aren't going to cut it. Waaah. Jerry L. Ricks Research Scientist University of Washington Department of Pathology _________________________________________________________________ See what people are saying about Windows Live. Check out featured posts. http://www.windowslive.com/connect?ocid=TXT_TAGLM_WL_connect2_082008 From kbowden <@t> ucsd.edu Thu Aug 21 18:12:56 2008 From: kbowden <@t> ucsd.edu (kbowden) Date: Thu Aug 21 18:13:03 2008 Subject: [Histonet] This Hematoxylin Shortage is starting to really hurt! In-Reply-To: References: Message-ID: <48ADF678.7000504@ucsd.edu> I just ordered Hematoxylin powder from Fisher about two weeks ago and I got it today. -- Karen Bowden Staff Research Associate II University of CA, San Diego Department of Orthopedics 9500 Gilman Dr. 0630 La Jolla, CA 92093-0630 858-534-4655 voice 858-534-5304 fax CONFIDENTIALITY NOTICE: THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED. IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND DELETE THE MATERIAL FROM ANY COMPUTER. JR R wrote: > I ordered Hematoxylin powder fromg Sogma *Catalog H-3136) In Early April--they said it was backordered. I still haven't received my powder, and I'm about to run out. > > I am doing large volume Movat Pentachrome Staining and the Hematoxylin substitutes aren't going to cut it. > > Waaah. > > > Jerry L. Ricks > Research Scientist > University of Washington > Department of Pathology > _________________________________________________________________ > See what people are saying about Windows Live. Check out featured posts. > http://www.windowslive.com/connect?ocid=TXT_TAGLM_WL_connect2_082008_______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From AnthonyH <@t> chw.edu.au Thu Aug 21 18:17:14 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Aug 21 18:17:28 2008 Subject: [Histonet] (para)formaldehyde (was: in situ question) In-Reply-To: <006e01c9036a$e2abb3d0$97a5d680@mmkem12636> Message-ID: Why would it be different? I am intrigued as to why IPX and ISH would reaxct differently in tissues fixed in either. Do you have any references? Or please write up your results and publish them (J Histotechnology or Biotechnic & Histochem are definitely worth considering). Often there will be differences but my experience indicates that it is often due to the fixation time. 10% buffered formalin is usually used at room temp, overnight at least, where as researchers for some reason often use 4% formaldehyde (made from polyformaldehyde) at 4oC for only a few hours at best. So often fixation occurs in the ethanols in processing. For valid comparisons fixation conditions must be standardised. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mikael Niku Sent: Thursday, 21 August 2008 6:50 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (para)formaldehyde (was: in situ question) This has probably been discussed 1000s of times, but.... I'm wondering to what extent dissolved paraformaldehyde really is equivalent to 4% formaldehyde or 10% formalin. In my experience, samples fixed in formalin and "4% PFA" as we tend to say are different when one does immunostaining or especially in situ hybridization. Formalin is of course traditionally used in pathology, whereas many labs doing immunos or ISH use "PFA". But what really causes the differences? Is it incomplete de-polymerization of paraformaldehyde, or perhaps the methanol typically included in formalin? ----------------------------------------------------- Mikael Niku, PhD, university lecturer University of Helsinki, Division of Nutrition URL: mikael.nikunnakki.info - What do I think of western civilization? I think it would be a good idea! Gandhi ----------------------------------------------------- -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: 20. elokuuta 2008 4:42 To: Lee Crosby; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] in situ question Which the becomes 4% formaldehyde or equivalent to 10% formalin. Not 4% paraformaldehyde. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From jnocito <@t> satx.rr.com Thu Aug 21 18:26:10 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Aug 21 18:26:03 2008 Subject: [Histonet] Flaming Friday Subject References: <236614.62990.qm@web65715.mail.ac4.yahoo.com> Message-ID: <89A01D844FDC4A2FB9B34E1828A77572@yourxhtr8hvc4p> how about demoing a tissue microwave oven and the magnetron goes out and annihilates your tissue JTT ----- Original Message ----- From: "Rene J Buesa" To: "Breeden, Sara" ; ; "Rathborne, Toni" Sent: Thursday, August 21, 2008 3:27 PM Subject: Re: [Histonet] Flaming Friday Subject Don't even think in buying it or "a like substitute" Ren? J. --- On Thu, 8/21/08, Rathborne, Toni wrote: From: Rathborne, Toni Subject: [Histonet] Flaming Friday Subject To: "Breeden, Sara" , histonet@lists.utsouthwestern.edu Date: Thursday, August 21, 2008, 4:03 PM How about demo units that are non-functioning? CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Thu Aug 21 18:30:11 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Aug 21 18:30:20 2008 Subject: [Histonet] Hyaluronidase In-Reply-To: <0MKp8S-1KWHTP2zxq-0003wW@mrelay.perfora.net> Message-ID: William, Hyaluronidase, to my knowledge has not been successfully used to unmask antigens like Proteinase K and trypsin. It has been successfully used in Mesothelioma immunohistochemistry to remove hyaluronic acid prior to immunostaining. Sometimes a background staining with anti CEA antibodies can occur if there is an abundant of hyaluronic acid present. Staining was cleaned-up after hyaluronidase pre-treatment. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of William Connor Sent: Friday, 22 August 2008 7:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hyaluronidase Greetings, How interchangeable are hyaluronidase and Proteinase K for an IHC procedure? If the protocol calls for hyaluronidase, had I better use it specifically? William P. Connor, HT(ASCP) Senior Histology Technician Histology Tech Services, Inc. This email and its attachments (if any) contain confidential and privileged information. Any dissemination or use of this information by a person other than the intended recipient is unauthorized and may be illegal. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From louise.renton <@t> gmail.com Fri Aug 22 04:35:51 2008 From: louise.renton <@t> gmail.com (louise renton) Date: Fri Aug 22 04:35:58 2008 Subject: [Histonet] mortuary specifications? Message-ID: Hi all, not sure if this is the right forum, but perhaps somebody out there could help or point me in the right direction. A friend of mine is involved in risk assessment for a medico legal morgue/mortuary and also providing them with possible solutions for their current problems - odour, fumes etc. My question is this - is there a guideline that anyone knows of for mortuary construction/design? OSHA? CAP? someone else? I would appreciate any responses to this best regards and have a great weekend. PS i will be doing the frozen haem experiment next week. will let u all in on the results -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Aug 22 05:56:41 2008 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Aug 22 05:56:46 2008 Subject: [Histonet] mortuary specifications? Message-ID: <86ADE4EB583CE64799A9924684A0FBBF052B946F@wahtntex2.waht.swest.nhs.uk> You ought to have downdraught mortuary tables made of stainless steel; that's the stuff we use in the UK. Downdraught autopsy tables will help to reduce smells and take away some of the dangerous pathogens; the tables need to be properly filtered and vented. Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Don't be afraid to take a big step when one is indicated. You can't cross a chasm in two small jumps. --Buckminster Fuller This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From JMyers1 <@t> aol.com Fri Aug 22 05:59:53 2008 From: JMyers1 <@t> aol.com (JMyers1@aol.com) Date: Fri Aug 22 06:00:00 2008 Subject: [Histonet] Retriever 2100 Message-ID: Belinda: The Retriever was originally developed for sterilizing surgical instruments in a physicians office setting. Although its easy to operate and costs less than a decloaking chamber, it doesn't monitor or display the operating temperature, lacks a pressure gauge (which provides performance verification), and you can't modify the time or temperature settings. It may be marketed for retrieval, but when you consider its limitations and its pricetag, it just doesn't measure up. I thoroughly evaluated the Retriever before including it in a review of retrieval methods and devices that was published a few years ago. For more information, consider downloading the paper, available through this link: _http://www.mlo-online.com/articles/0606/0606cover_story.pdf_ (http://www.mlo-online.com/articles/0606/0606cover_story.pdf) ). Good Luck, Joe ------------------------------ Message: 3 Date: Wed, 20 Aug 2008 13:59:01 -0500 From: "McMahan, Belinda" Subject: [Histonet] Retriever 2100 To: Good afternoon, Has anyone used the Retriever 2100 from EMS? It is a pressure cooker for antigen retrieval and I would like to know how it compares to the Decloaking Chamber from Biocare which is what I currently use. Any information will be helpful. Thanks, Belinda McMahan Washington University Dept. of Ophthalmology Immunomorphology Core Lab **************It's only a deal if it's where you want to go. Find your travel deal here. (http://information.travel.aol.com/deals?ncid=aoltrv00050000000047) From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Aug 22 06:47:50 2008 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Aug 22 06:47:56 2008 Subject: [Histonet] Retriever 2100 Message-ID: <86ADE4EB583CE64799A9924684A0FBBF052B9478@wahtntex2.waht.swest.nhs.uk> "Although its easy to operate and costs less than a decloaking chamber," Romulan? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Don't be afraid to take a big step when one is indicated. You can't cross a chasm in two small jumps. --Buckminster Fuller This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From rjbuesa <@t> yahoo.com Fri Aug 22 07:26:42 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 22 07:26:49 2008 Subject: [Histonet] Hyaluronidase In-Reply-To: <0MKp8S-1KWHTP2zxq-0003wW@mrelay.perfora.net> Message-ID: <877063.81033.qm@web65709.mail.ac4.yahoo.com> They are not. Ren? J. --- On Thu, 8/21/08, William Connor wrote: From: William Connor Subject: [Histonet] Hyaluronidase To: histonet@lists.utsouthwestern.edu Date: Thursday, August 21, 2008, 5:13 PM Greetings, How interchangeable are hyaluronidase and Proteinase K for an IHC procedure? If the protocol calls for hyaluronidase, had I better use it specifically? William P. Connor, HT(ASCP) Senior Histology Technician Histology Tech Services, Inc. This email and its attachments (if any) contain confidential and privileged information. Any dissemination or use of this information by a person other than the intended recipient is unauthorized and may be illegal. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Aug 22 07:30:14 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 22 07:30:16 2008 Subject: [Histonet] counterstain on bloodsmear after DAB In-Reply-To: <425949.45524.qm@web38002.mail.mud.yahoo.com> Message-ID: <71891.55938.qm@web65715.mail.ac4.yahoo.com> Do as you have been doing (Hematox), because you are not trying to study the blood cells after all. Ren? J. --- On Thu, 8/21/08, Amy Lee wrote: From: Amy Lee Subject: [Histonet] counterstain on bloodsmear after DAB To: "histonet" Date: Thursday, August 21, 2008, 6:22 PM Hello, Could anybody tell me what kind of counterstain you use for blood smear after DAB? I used to use hemotoxyline on tissues but this time it's blood smear. ? Any advice is highly appreciated! ? Amy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From newacct391924 <@t> aol.com Fri Aug 22 08:18:53 2008 From: newacct391924 <@t> aol.com (newacct391924@aol.com) Date: Fri Aug 22 08:19:01 2008 Subject: [Histonet] (no subject) Message-ID: i'd like to post to this list newacct391924@aol.com **************It's only a deal if it's where you want to go. Find your travel deal here. (http://information.travel.aol.com/deals?ncid=aoltrv00050000000047) From lblazek <@t> digestivespecialists.com Fri Aug 22 08:52:27 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Aug 22 08:53:11 2008 Subject: [Histonet] job opening Message-ID: <5A2BD13465E061429D6455C8D6B40E390D664E94@IBMB7Exchange.digestivespecialists.com> We're growing and have a job opening for a full time ASCP certified tech. We are located in Huber Heights Ohio (near Dayton). We are a private, fast growing fully automated state of the art GI lab. This is a Monday thru Friday day shift position. No weekends or holidays! We offer full benefits, and excellent pay, while working with a great bunch of people. Call or email me for more information. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com From Heather.D.Renko <@t> osfhealthcare.org Fri Aug 22 12:17:05 2008 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Fri Aug 22 12:17:17 2008 Subject: [Histonet] RE: hematoxylin shortage Message-ID: <40026EDDE64CDA47AB382C52619ACD3C0C44A052@pmc-rfd-mx01.intranet.osfnet.org> If its of any conciliation, I Just spoke with my rep from ThermoFisher/old RA rep and she said that they are getting a supply in from Merck (I believe) and that there should not be any more shortage issues from them?? She is a genuinely nice and honest sales rep so, only Time will tell-I will keep my synthetically stained fingers crossed and go from there. Happy Friday!! Darn those hurricanes knocking down our trees.... Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From Sharon.Davis-Devine <@t> carle.com Fri Aug 22 12:23:09 2008 From: Sharon.Davis-Devine <@t> carle.com (Sharon.Davis-Devine) Date: Fri Aug 22 12:23:14 2008 Subject: [Histonet] Training for gross Message-ID: <44780C571F28624DBB446DE55C4D733A021E0A74@EXCHANGEBE1.carle.com> For all of you Histonetters out there I have a question about training individuals to gross in small specimens. Can a person with a degree and being a Cytotechnologist, Medical Technologist or a Histotechnologist gross in small biopsy samples? And if they can, what kind of training is required and for how long? We are losing one of our PA's and are contemplating replacing that person with a person with a degree. Thanks for the info. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com From liz <@t> premierlab.com Fri Aug 22 12:24:36 2008 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Aug 22 12:24:41 2008 Subject: [Histonet] RE: hematoxylin shortage In-Reply-To: <40026EDDE64CDA47AB382C52619ACD3C0C44A052@pmc-rfd-mx01.intranet.osfnet.org> References: <40026EDDE64CDA47AB382C52619ACD3C0C44A052@pmc-rfd-mx01.intranet.osfnet.org> Message-ID: I have tried to order from VWR and the last two times they canceled the order, stating that the item has been discontinued. I have another order placed through VWR with a different vendor and one with Sigma for over a month and so far have not received any hematoxylin powder yet. I have not been able to get any for about 3 months now. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Renko, Heather D. Sent: Friday, August 22, 2008 11:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: hematoxylin shortage If its of any conciliation, I Just spoke with my rep from ThermoFisher/old RA rep and she said that they are getting a supply in from Merck (I believe) and that there should not be any more shortage issues from them?? She is a genuinely nice and honest sales rep so, only Time will tell-I will keep my synthetically stained fingers crossed and go from there. Happy Friday!! Darn those hurricanes knocking down our trees.... Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ======================================================================== ====== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Heather.D.Renko <@t> osfhealthcare.org Fri Aug 22 12:53:16 2008 From: Heather.D.Renko <@t> osfhealthcare.org (Renko, Heather D.) Date: Fri Aug 22 12:53:32 2008 Subject: [Histonet] Re: mortuary Specifications Message-ID: <40026EDDE64CDA47AB382C52619ACD3C0C44A053@pmc-rfd-mx01.intranet.osfnet.org> Kemlo, Here in the states the IDPH is ultimately who we have to answer to..... but the Clinical Laboratory Standards Institute guidelines generally would meet all regulatory requirements. They address autopsy suites in several of the areas, I have the PDF of the latest edition if you would like me to send it to you(120 pages). "Laboratory Design, Approved Guidelines", Clinical Laboratory Standards Institute, 2nd Edition;Vol 27 No. 7. Hope this is helpful! Heather D. Renko, Histology Coordinator OSF Saint Anthony Medical Center 5666 East State Street Rockford, Illinois 61108 Direct: 815-395-5410 Heather.D.Renko@osfhealthcare.org ============================================================================== The information in this message is confidential and may be legally privileged. Access to this message by anyone other than the addressee is not authorized. If you are not the intended recipient, or an agent of the intended recipient, any disclosure, copying, or distribution of the message or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. If you have received this message in error, please contact the sender immediately and permanently delete the original e-mail, attachment(s), and any copies. ============================================================================== From Stephen.Clark1 <@t> hcahealthcare.com Fri Aug 22 13:02:24 2008 From: Stephen.Clark1 <@t> hcahealthcare.com (Clark Stephen - Myrtle Beach) Date: Fri Aug 22 13:02:45 2008 Subject: [Histonet] Training for gross In-Reply-To: <44780C571F28624DBB446DE55C4D733A021E0A74@EXCHANGEBE1.carle.com> References: <44780C571F28624DBB446DE55C4D733A021E0A74@EXCHANGEBE1.carle.com> Message-ID: <28E40C736ED32341BBB2B094EFD23FC8020F0741@NASEV05.hca.corpad.net> Also, in regards to this question, what is designated as a "small" specimen? Steve Clark Histology Supervisor Grand Strand Reg. Medical Ctr. Myrtle Beach, SC (843) 692-1486 Stephen.Clark1@hcahealthcare.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Friday, August 22, 2008 1:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Training for gross For all of you Histonetters out there I have a question about training individuals to gross in small specimens. Can a person with a degree and being a Cytotechnologist, Medical Technologist or a Histotechnologist gross in small biopsy samples? And if they can, what kind of training is required and for how long? We are losing one of our PA's and are contemplating replacing that person with a person with a degree. Thanks for the info. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Shirley_PHUA <@t> hsa.gov.sg Fri Aug 22 13:03:00 2008 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Fri Aug 22 13:04:04 2008 Subject: [Histonet] Shirley Phua is out-of-office ... Message-ID: I will be out of the office from 22-08-2008 to 23-08-2008. I'll be away on course 25 Aug - 05 Sep 2008. Pathologists: I will process your requests when I return. If urgent, please forward your email to Henry_Kyaw@hsa.gov.sg From lblazek <@t> digestivespecialists.com Fri Aug 22 13:15:36 2008 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Aug 22 13:16:15 2008 Subject: [Histonet] Training for gross In-Reply-To: <28E40C736ED32341BBB2B094EFD23FC8020F0741@NASEV05.hca.corpad.net> References: <44780C571F28624DBB446DE55C4D733A021E0A74@EXCHANGEBE1.carle.com> <28E40C736ED32341BBB2B094EFD23FC8020F0741@NASEV05.hca.corpad.net> Message-ID: <5A2BD13465E061429D6455C8D6B40E390D664E9B@IBMB7Exchange.digestivespecialists.com> You're losing a PA! Is Chuck leaving? Does he need a job back here in Ohio? Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Clark Stephen - Myrtle Beach Sent: Friday, August 22, 2008 2:02 PM To: Sharon.Davis-Devine; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Training for gross Also, in regards to this question, what is designated as a "small" specimen? Steve Clark Histology Supervisor Grand Strand Reg. Medical Ctr. Myrtle Beach, SC (843) 692-1486 Stephen.Clark1@hcahealthcare.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Friday, August 22, 2008 1:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Training for gross For all of you Histonetters out there I have a question about training individuals to gross in small specimens. Can a person with a degree and being a Cytotechnologist, Medical Technologist or a Histotechnologist gross in small biopsy samples? And if they can, what kind of training is required and for how long? We are losing one of our PA's and are contemplating replacing that person with a person with a degree. Thanks for the info. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Aug 22 13:43:12 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 22 13:43:15 2008 Subject: [Histonet] Training for gross In-Reply-To: <44780C571F28624DBB446DE55C4D733A021E0A74@EXCHANGEBE1.carle.com> Message-ID: <289927.89068.qm@web65709.mail.ac4.yahoo.com> Description ofno matter what type of specimen is considered a "high complexity task" and should be done by somebody with the required training. It is acceptable to have somebody with the qualifications you describe to be trained, but the training should be for the time required in the PA program. Another approach will be that the person is trained by another PA or by a pathologist. The best solution also would require that your medical director accepts the training type and period and that somebody supervises both the training and the descriptions done by the trainee during some period of time. I personally never used anybody but a PA or?a pathology resident to do the descriptions. Too many liability issues could be involved. Ren? J. --- On Fri, 8/22/08, Sharon.Davis-Devine wrote: From: Sharon.Davis-Devine Subject: [Histonet] Training for gross To: histonet@lists.utsouthwestern.edu Date: Friday, August 22, 2008, 1:23 PM For all of you Histonetters out there I have a question about training individuals to gross in small specimens. Can a person with a degree and being a Cytotechnologist, Medical Technologist or a Histotechnologist gross in small biopsy samples? And if they can, what kind of training is required and for how long? We are losing one of our PA's and are contemplating replacing that person with a person with a degree. Thanks for the info. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jryan <@t> sleh.com Fri Aug 22 15:40:51 2008 From: jryan <@t> sleh.com (Ryan, John P.) Date: Fri Aug 22 15:41:02 2008 Subject: [Histonet] bone marrow decalcification Message-ID: What method do you utilize for the decalcification of bone marrow specimens that does not impair immunohistochemical staining? We have noted that our immunos (not surprisingly) stain less intensely on decalcified bone marrow than on non-decalcified tissue and would like to minimize this effect. John Ryan St. Luke's Episcopal Hospital 832-355-2643 jryan@sleh.com +++++CONFIDENTIALITY NOTICE+++++ The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. From JWeems <@t> sjha.org Fri Aug 22 15:53:59 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Fri Aug 22 15:54:08 2008 Subject: [Histonet] bone marrow decalcification In-Reply-To: References: Message-ID: <982A0A9461F9BF438C7B19A6E425A383553771@ITSSSXM01V6.one.ads.che.org> B-Plus fixative from BBC Biochemical works well for us. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ryan, John P. Sent: Friday, August 22, 2008 4:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] bone marrow decalcification What method do you utilize for the decalcification of bone marrow specimens that does not impair immunohistochemical staining? We have noted that our immunos (not surprisingly) stain less intensely on decalcified bone marrow than on non-decalcified tissue and would like to minimize this effect. John Ryan St. Luke's Episcopal Hospital 832-355-2643 jryan@sleh.com +++++CONFIDENTIALITY NOTICE+++++ The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From rjbuesa <@t> yahoo.com Fri Aug 22 16:16:58 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 22 16:17:01 2008 Subject: [Histonet] bone marrow decalcification In-Reply-To: Message-ID: <144983.7759.qm@web65706.mail.ac4.yahoo.com> If you decalcify with EDTA the IHC staining will be good. Do NOT use RDO or any other strong acid. Ren? J. --- On Fri, 8/22/08, Ryan, John P. wrote: From: Ryan, John P. Subject: [Histonet] bone marrow decalcification To: "histonet@lists.utsouthwestern.edu" Date: Friday, August 22, 2008, 4:40 PM What method do you utilize for the decalcification of bone marrow specimens that does not impair immunohistochemical staining? We have noted that our immunos (not surprisingly) stain less intensely on decalcified bone marrow than on non-decalcified tissue and would like to minimize this effect. John Ryan St. Luke's Episcopal Hospital 832-355-2643 jryan@sleh.com +++++CONFIDENTIALITY NOTICE+++++ The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charlene.Henry <@t> STJUDE.ORG Fri Aug 22 16:21:25 2008 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Fri Aug 22 16:21:30 2008 Subject: [Histonet] bone marrow decalcification. . In-Reply-To: <982A0A9461F9BF438C7B19A6E425A383553771@ITSSSXM01V6.one.ads.che.org> Message-ID: <03E1F5968F60C5448635D49D38B283ED02723098F6@SJMEMXMBS11.stjude.sjcrh.local> John, We use B+ fixative and EDTA/Formalin to decalcify BM Bxs. and they works great for IHC and even ISH. Many times the marker is more intense on the EDTA decalcified tissues than just formalin fixed tissues. It varies from antibody to antibody. Charlene Henry HT (ASCP), QIHC Anatomic Pathology Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, August 22, 2008 3:54 PM To: Ryan, John P.; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] bone marrow decalcification. . B-Plus fixative from BBC Biochemical works well for us. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ryan, John P. Sent: Friday, August 22, 2008 4:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] bone marrow decalcification What method do you utilize for the decalcification of bone marrow specimens that does not impair immunohistochemical staining? We have noted that our immunos (not surprisingly) stain less intensely on decalcified bone marrow than on non-decalcified tissue and would like to minimize this effect. John Ryan St. Luke's Episcopal Hospital 832-355-2643 jryan@sleh.com +++++CONFIDENTIALITY NOTICE+++++ The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Shirley.Chu <@t> moldev.com Fri Aug 22 16:19:58 2008 From: Shirley.Chu <@t> moldev.com (Chu, Shirley) Date: Fri Aug 22 16:23:10 2008 Subject: [Histonet] Laser Capture Microdissection Webinar Message-ID: Announcing MDS Analytical Technologies next in the series of Laser Capture Microdissection and Microgenomics Webinars. Our next LCM webinar will be held on Wed, Aug. 27, 2008 at 10AM PDT or 1PM EDT. The presenter will be Mark Erlander, Ph.D., Chief Scientific Officer of AviaraDX. His presentation is titled: "Applying Paradise(r) Plus for the Molecular Classification of Cancers of Uncertain Primary Origin". For further information and/or to register for this webinar, please go to the following website: http://www.moleculardevices.com/pages/lcm_webinar_8.2008.html There is no cost to register and attend this webinar. Shirley Chu Application Scientist, Arcturus LCM Products Molecular Devices (now a part of MDS Analytical Technologies) 408-747-3765 | www.moleculardevices.com From marktarango <@t> gmail.com Fri Aug 22 16:32:36 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Fri Aug 22 16:32:42 2008 Subject: [Histonet] bone marrow decalcification In-Reply-To: References: Message-ID: <5b6eb13e0808221432o607fa81co1e445f9f8d75474@mail.gmail.com> Hi John, Are you sure that the BM biopsies are completely fixed before going into decal? No pinkish or reddish-colored cores should be put into decal. I'd look into the fixation time before decalcification. If you can increase this, it might be all that you need to do. Mark On 8/22/08, Ryan, John P. wrote: > What method do you utilize for the decalcification of bone marrow specimens that does not impair immunohistochemical staining? We have noted that our immunos (not surprisingly) stain less intensely on decalcified bone marrow than on non-decalcified tissue and would like to minimize this effect. > > John Ryan > St. Luke's Episcopal Hospital > 832-355-2643 > jryan@sleh.com > > > +++++CONFIDENTIALITY NOTICE+++++ > The information in this e-mail may be confidential and/or > privileged. If you are not the intended recipient or an > authorized representative of the intended recipient, you > are hereby notified that any review, dissemination or > copying of this e-mail and its attachments, if any, or the > information contained herein is prohibited. If you have > received this e-mail in error, please immediately notify > the sender by return e-mail and delete this e-mail from > your computer system. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Lia.Caldwell <@t> TriadHospitals.com Fri Aug 22 16:54:02 2008 From: Lia.Caldwell <@t> TriadHospitals.com (Caldwell, Lia) Date: Fri Aug 22 16:54:19 2008 Subject: [Histonet] Training for gross References: <44780C571F28624DBB446DE55C4D733A021E0A74@EXCHANGEBE1.carle.com> Message-ID: <774F7DC732C89743BB070F1E87D402541A81A0@TNTRIEXEVS04.triadhospitals.net> Sharon, This came up during a recent CAP inspection of a lab we inspected in Maricopa so I have taken the liberty of copying the information from the CAP guidelines regarding this issue for you. The first question defines high complexity testing and the second question addresses requirements for individuals performing these tasks. Hope this helps, have a great weekend! ~Lia According to CAP guidelines ANP.11600 "Are all macroscopic tissue examinations performed by a pathologist or pathology resident, or under supervision of a qualified pathologist? Note: Two levels of complexity of macroscopic tissue examination are defined, as follows: 1. Processing is defined as a tissue examination limited to description, inking and cutting of the specimen (if applicable), and submission of the entire specimen to histology. Tissue processing can be performed accroding to standardized protocols. Processing is generally limited to small specimens (skin ellipses, small biopsies, curettings, etc.) and does not require knowledge of anatomy. 2. Grossing (or gross examination) is defined as a tissue examination requiring a greater exercise of judgement and a knowledge of anatomy. Dissection of the specimen and selection of tissue samples for submission to histology are generally required. The specimen description is not necessarily stadardized. ANP.11610 "If individuals other than a pathologist or pathology resident assist in gross examinations, do such individuals qualify as high complexity testing personnel under CLIA-88 regulations? NOTE: The laboratory director may delegate the dissection of specimens to non-pathologist individuals; these individuals must be qualified as high complexity testing personnel under CLIA-88 regulations. The minimum training/experience required of such personnel is: 1. An earned associate degree in a laboratory science or medical laboratory technology, obtained from an accredited institution, OR 2. Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education must include 24 semester hours of medical laboratory technology courses, OR 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. In addition, the individual must have laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, the CAHEA, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), OR at least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. In addition, the CLIA-88 regulations include exceptions for grandfathered individuals; these regulations (42CFR493.1489 and 1491) may be found at http://www.phppo.cdc.gov/clia/regs/subpart_m.aspx#493.1487 It is the responsibility of the laboratory director to determine whether an individual's education, training and experience satisfies the requirements of this checklist question. This checklist question applies only to laboratories subject to CLIA-88 Lia M. Caldwell HT (ASCP) Histology Supervisor Oro Valley Pathology Dept. phone: (520) 901-3914 www.Lia.Caldwell@TriadHospitals.com "be yourself - everyone else is already taken." -unknown ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sharon.Davis-Devine Sent: Fri 8/22/2008 10:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Training for gross For all of you Histonetters out there I have a question about training individuals to gross in small specimens. Can a person with a degree and being a Cytotechnologist, Medical Technologist or a Histotechnologist gross in small biopsy samples? And if they can, what kind of training is required and for how long? We are losing one of our PA's and are contemplating replacing that person with a person with a degree. Thanks for the info. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From carlos <@t> clubstaffing.com Fri Aug 22 16:59:08 2008 From: carlos <@t> clubstaffing.com (Carlos Stolk) Date: Fri Aug 22 16:59:14 2008 Subject: [Histonet] Training for gross In-Reply-To: <774F7DC732C89743BB070F1E87D402541A81A0@TNTRIEXEVS04.triadhospitals.net> References: <44780C571F28624DBB446DE55C4D733A021E0A74@EXCHANGEBE1.carle.com> <774F7DC732C89743BB070F1E87D402541A81A0@TNTRIEXEVS04.triadhospitals.net> Message-ID: This is getting a little bit complicated. We should talk about this over a nice steak dinner. Have a splendid weekend Carlos Stolk Account Representative Georgia | New Mexico South Carolina| Texas Phone: 800-875-8999 ext. 258 Fax: 561-367-0884 carlos@clubstaffing.com Club Staffing, Inc. 5901 Broken Sound Pkwy, Ste 500 Boca Raton, FL 33487 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Caldwell, Lia Sent: Friday, August 22, 2008 5:54 PM To: Sharon.Davis-Devine Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Training for gross Sharon, This came up during a recent CAP inspection of a lab we inspected in Maricopa so I have taken the liberty of copying the information from the CAP guidelines regarding this issue for you. The first question defines high complexity testing and the second question addresses requirements for individuals performing these tasks. Hope this helps, have a great weekend! ~Lia According to CAP guidelines ANP.11600 "Are all macroscopic tissue examinations performed by a pathologist or pathology resident, or under supervision of a qualified pathologist? Note: Two levels of complexity of macroscopic tissue examination are defined, as follows: 1. Processing is defined as a tissue examination limited to description, inking and cutting of the specimen (if applicable), and submission of the entire specimen to histology. Tissue processing can be performed accroding to standardized protocols. Processing is generally limited to small specimens (skin ellipses, small biopsies, curettings, etc.) and does not require knowledge of anatomy. 2. Grossing (or gross examination) is defined as a tissue examination requiring a greater exercise of judgement and a knowledge of anatomy. Dissection of the specimen and selection of tissue samples for submission to histology are generally required. The specimen description is not necessarily stadardized. ANP.11610 "If individuals other than a pathologist or pathology resident assist in gross examinations, do such individuals qualify as high complexity testing personnel under CLIA-88 regulations? NOTE: The laboratory director may delegate the dissection of specimens to non-pathologist individuals; these individuals must be qualified as high complexity testing personnel under CLIA-88 regulations. The minimum training/experience required of such personnel is: 1. An earned associate degree in a laboratory science or medical laboratory technology, obtained from an accredited institution, OR 2. Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education must include 24 semester hours of medical laboratory technology courses, OR 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. In addition, the individual must have laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, the CAHEA, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), OR at least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. In addition, the CLIA-88 regulations include exceptions for grandfathered individuals; these regulations (42CFR493.1489 and 1491) may be found at http://www.phppo.cdc.gov/clia/regs/subpart_m.aspx#493.1487 It is the responsibility of the laboratory director to determine whether an individual's education, training and experience satisfies the requirements of this checklist question. This checklist question applies only to laboratories subject to CLIA-88 Lia M. Caldwell HT (ASCP) Histology Supervisor Oro Valley Pathology Dept. phone: (520) 901-3914 www.Lia.Caldwell@TriadHospitals.com "be yourself - everyone else is already taken." -unknown ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sharon.Davis-Devine Sent: Fri 8/22/2008 10:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Training for gross For all of you Histonetters out there I have a question about training individuals to gross in small specimens. Can a person with a degree and being a Cytotechnologist, Medical Technologist or a Histotechnologist gross in small biopsy samples? And if they can, what kind of training is required and for how long? We are losing one of our PA's and are contemplating replacing that person with a person with a degree. Thanks for the info. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Fri Aug 22 17:37:05 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Aug 22 17:36:33 2008 Subject: [Histonet] bone marrow decalcification References: <982A0A9461F9BF438C7B19A6E425A383553771@ITSSSXM01V6.one.ads.che.org> Message-ID: <604D83B129B444C785B2834DFF42A848@yourxhtr8hvc4p> Biocare Medical has ionic beads that attracts the calcium out from the specimen. You can use it up to 20 blocks, is really inexpensive and does improve your immunos. JTT ----- Original Message ----- From: "Weems, Joyce" To: "Ryan, John P." ; Sent: Friday, August 22, 2008 3:53 PM Subject: RE: [Histonet] bone marrow decalcification B-Plus fixative from BBC Biochemical works well for us. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ryan, John P. Sent: Friday, August 22, 2008 4:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] bone marrow decalcification What method do you utilize for the decalcification of bone marrow specimens that does not impair immunohistochemical staining? We have noted that our immunos (not surprisingly) stain less intensely on decalcified bone marrow than on non-decalcified tissue and would like to minimize this effect. John Ryan St. Luke's Episcopal Hospital 832-355-2643 jryan@sleh.com +++++CONFIDENTIALITY NOTICE+++++ The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From msviapiano <@t> yahoo.com Sat Aug 23 11:39:27 2008 From: msviapiano <@t> yahoo.com (Mariano S. Viapiano) Date: Sat Aug 23 11:39:31 2008 Subject: [Histonet] Anti KI-67 (rat) Message-ID: <522665.96396.qm@web54507.mail.re2.yahoo.com> Hello all, Could anybody recommend a good antibody to detect Ki-67 in rat tissues (frozen) by fluorescence IHC? Thank you! Regards, Mariano Mariano S. Viapiano, PhD Assistant Professor Center for Molecular Neurobiology and Department of Neurological Surgery The Ohio State University 226B Rightmire Hall 1060 Carmack Rd., Columbus OH 43210 Tel (614) 292-4362 Fax (614) 292-5379 From Eric.Hoy <@t> UTSouthwestern.edu Sat Aug 23 15:08:00 2008 From: Eric.Hoy <@t> UTSouthwestern.edu (Eric Hoy) Date: Sat Aug 23 15:08:10 2008 Subject: [Histonet] Re: Histonet Digest, Vol 57, Issue 32 In-Reply-To: <200808221700.m7MH0Xwv009234@flpi114.prodigy.net> Message-ID: On 8/22/08 12:00 PM, "histonet-request@lists.utsouthwestern.edu" wrote: > From: "Joe Nocito" > Subject: Re: [Histonet] Flaming Friday Subject > > how about demoing a tissue microwave oven and the magnetron goes out and > annihilates your tissue Well, at least you were using it in the "use for which it was intended". Not like one of those dangerous household microwaves! Eric Hoy =============================================== Eric S. Hoy, Ph.D., SI(ASCP) Clinical Associate Professor Department of Medical Laboratory Sciences The University of Texas Southwestern Medical Center Dallas, Texas Email: Eric.Hoy@UTSouthwestern.edu =============================================== From Erin.Martin <@t> ucsf.edu Sat Aug 23 19:04:46 2008 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Sat Aug 23 19:05:43 2008 Subject: [Histonet] hematoxylin shortage Message-ID: My rep from thermo fisher told me last week that they expect supplies to be returning to normal soon. From sjchtascp <@t> yahoo.com Sat Aug 23 21:38:08 2008 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Sat Aug 23 21:38:13 2008 Subject: [Histonet] Looking for work in NH Message-ID: <969864.54596.qm@web38207.mail.mud.yahoo.com> I'm looking for an HT position in New Hampsire ? Steve From amosbrooks <@t> gmail.com Sun Aug 24 10:27:57 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sun Aug 24 10:28:05 2008 Subject: [Histonet] Anti KI-67 (rat) Message-ID: <582736990808240827g5a05c979i833afd165c80aae1@mail.gmail.com> Mariano, Labvision has a very nice Rabbit Monoclonal antibody that works a charm. It is RM-9106, but it looks like any of the polyclonal antibodies should work in rat. Check out the following link: http://www.labvision.com/ab1.cfm?First=AntiBody&Second=Ki67 Good Luck, Amos Message: 18 Date: Sat, 23 Aug 2008 09:39:27 -0700 (PDT) From: "Mariano S. Viapiano" Subject: [Histonet] Anti KI-67 (rat) To: Histonet@lists.utsouthwestern.edu Message-ID: <522665.96396.qm@web54507.mail.re2.yahoo.com> Content-Type: text/plain; charset=us-ascii Hello all, Could anybody recommend a good antibody to detect Ki-67 in rat tissues (frozen) by fluorescence IHC? Thank you! Regards, Mariano From webb3655 <@t> sbcglobal.net Sun Aug 24 20:55:50 2008 From: webb3655 <@t> sbcglobal.net (judy webb) Date: Sun Aug 24 20:55:53 2008 Subject: [Histonet] Job Opening Message-ID: <75477.11301.qm@web83606.mail.sp1.yahoo.com> Histonet, John Peter Smith Hospital, Fort Worth Texas has a new position for a Histotech, HT(ASCP) , or eligible, added this summer. JPS is a busy county hospital offering routine histology, special stains, frozen sections and IHC. We have a great pathology staff and "state of the art" equipment. This position will be 8:30am to 5:00pm Monday-Friday,(no Saturdays). If interested please contact: Judy McKinney, HTL(ASCP) Technical Coordinator, Pathology Laboratory John Peter Smith Hospital 1500 S. Main Fort Worth, Texas 817-927-1024 jwebb01@jpshealth.org From algranth <@t> u.arizona.edu Mon Aug 25 10:05:57 2008 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Mon Aug 25 10:05:47 2008 Subject: [Histonet] PSLIM In-Reply-To: <71320B4EBC7C15419563EAFBFCD924651EDA553E6F@hades.pa-ucl.co m> References: <00d601c8fd89$86e06960$94a13c20$@com> <71320B4EBC7C15419563EAFBFCD924651EDA553E6F@hades.pa-ucl.com> Message-ID: <6.2.3.4.1.20080825080252.0270a770@algranth.inbox.email.arizona.edu> Good Monday Morning! So did we ever discover anybody who actually has this printer in their lab and is using it? I think we all saw it at a show this spring or summer but who bought one? We are coming real close to buying one but I'd really like to hear from a user who would hopefully tell me that it is as wonderful as it seems and the codesoft software is a dream to use. Andi Grantham ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From victor <@t> pathology.washington.edu Mon Aug 25 10:21:30 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Mon Aug 25 10:21:39 2008 Subject: [Histonet] PSLIM In-Reply-To: <6.2.3.4.1.20080825080252.0270a770@algranth.inbox.email.arizona.edu> References: <00d601c8fd89$86e06960$94a13c20$@com> <71320B4EBC7C15419563EAFBFCD924651EDA553E6F@hades.pa-ucl.com> <6.2.3.4.1.20080825080252.0270a770@algranth.inbox.email.arizona.edu> Message-ID: <48B2CDFA.8000103@pathology.washington.edu> We have ordered one and it should ship next week. I'll keep you posted on the product. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Andrea Grantham wrote: > Good Monday Morning! > So did we ever discover anybody who actually has this printer in their > lab and is using it? > I think we all saw it at a show this spring or summer but who bought one? > We are coming real close to buying one but I'd really like to hear > from a user who would hopefully tell me that it is as wonderful as it > seems and the codesoft software is a dream to use. > > Andi Grantham > ..................................................................... > : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : > : Sr. Research Specialist University of Arizona : > : (office: AHSC 4212) P.O. Box 245044 : > : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : > : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : > :...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JHAPPEL <@t> PARTNERS.ORG Mon Aug 25 10:29:59 2008 From: JHAPPEL <@t> PARTNERS.ORG (Happel, James F.) Date: Mon Aug 25 10:30:07 2008 Subject: [Histonet] Grossing Positions in Boston, Massachusetts Message-ID: Good Morning Histonetters, I have 2 to 3 openings for experienced grossing techs for a busy lab (mostly derm path) in the Boston, Massachusetts area. Early afternoon shifts (11:00 a.m. - 7:30 p.m. M-F). Please feel free to contact me at jhappel@pathsrv.com or call (617) 679-4143. Thanks and have a great day! James The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. From ploykasek <@t> phenopath.com Mon Aug 25 11:09:05 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Mon Aug 25 11:09:25 2008 Subject: [Histonet] PSLIM In-Reply-To: <6.2.3.4.1.20080825080252.0270a770@algranth.inbox.email.arizona.edu> Message-ID: You can get sample slides from this company. I would recommend you get sample slides, put them through all your pretreatments, and be sure the print is still legible after pretreatment. Patti Loykasek BS, HTL, QIHC Clinical IHC Supervisor PhenoPath Laboratories Seattle, WA > Good Monday Morning! > So did we ever discover anybody who actually has this printer in > their lab and is using it? > I think we all saw it at a show this spring or summer but who bought one? > We are coming real close to buying one but I'd really like to hear > from a user who would hopefully tell me that it is as wonderful as it > seems and the codesoft software is a dream to use. > > Andi Grantham > ..................................................................... > : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : > : Sr. Research Specialist University of Arizona : > : (office: AHSC 4212) P.O. Box 245044 : > : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : > : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : > :...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From victor <@t> pathology.washington.edu Mon Aug 25 11:16:08 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Mon Aug 25 11:16:13 2008 Subject: [Histonet] PSLIM In-Reply-To: References: Message-ID: <48B2DAC8.1010900@pathology.washington.edu> Patti, That is what we did and the slides still looked great and the bar code scanned easily. They have a list of slides that work well, ink does not come off. The slides we are using have not been tested extensively, but should be OK. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Patti Loykasek wrote: > You can get sample slides from this company. I would recommend you get > sample slides, put them through all your pretreatments, and be sure the > print is still legible after pretreatment. > > > > Patti Loykasek BS, HTL, QIHC > Clinical IHC Supervisor > PhenoPath Laboratories > Seattle, WA > > > > > > >> Good Monday Morning! >> So did we ever discover anybody who actually has this printer in >> their lab and is using it? >> I think we all saw it at a show this spring or summer but who bought one? >> We are coming real close to buying one but I'd really like to hear >> from a user who would hopefully tell me that it is as wonderful as it >> seems and the codesoft software is a dream to use. >> >> Andi Grantham >> ..................................................................... >> : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : >> : Sr. Research Specialist University of Arizona : >> : (office: AHSC 4212) P.O. Box 245044 : >> : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : >> : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : >> :...................................................................: >> http://www.cba.arizona.edu/histology-lab.html >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > > > This e-mail message, including any attachments, is for the sole use of the > intended recipients and may contain privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not the intended > recipient, please contact the sender by e-mail and destroy all copies of the > original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. > at (206) 374-9000. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From golder <@t> mail.nih.gov Mon Aug 25 13:19:52 2008 From: golder <@t> mail.nih.gov (Eric Gold) Date: Mon Aug 25 13:20:00 2008 Subject: [Histonet] X-Gal Rat Brain Message-ID: <97F03AD4-CB3C-440B-A5B0-D374C0DFEFC1@mail.nih.gov> Hi everybody, I had a question regarding fixing rat brain. We are currently perfusing and fixing with a 4% para / 3% sucrose solution. I am trying to get X-Gal staining, but I have read this may be undetectable once tissue has been paraffin embedded. First off, has anybody had luck with paraffinized tissue and x-gal? Secondly, we wanted to snap freeze and section the tissue ourselves if paraffin was not an option. From what I've tried thus far, I am not getting the structural integrity that comes from paraffinzed tissue. I know you are never going to get as good slides when frozen, but I think it should look more intact than what I am seeing. The tissue looks stretched out and very gappy. Could one of the reasons be that the tissue is being overfixed? The tissue has been sitting in the para/sucrose sol'n for anywhere from 3 days to 4 weeks. Maybe my snap freezing technique is off, but I don't think ice crystallization is the problem. Is there any advantage to using the para/sucrose sol'n to fix, or is it just better to fix overnight, move to sucrose gradient and then snap freeze? Thanks in advance for suggestions. -Eric From anh2006 <@t> med.cornell.edu Mon Aug 25 13:37:31 2008 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Mon Aug 25 13:37:42 2008 Subject: [Histonet] X-Gal Rat Brain In-Reply-To: <97F03AD4-CB3C-440B-A5B0-D374C0DFEFC1@mail.nih.gov> References: <97F03AD4-CB3C-440B-A5B0-D374C0DFEFC1@mail.nih.gov> Message-ID: I work with mouse and human not rat but the principles are the same ....... For X-Gal staining, PFA isn't the way to go. PFA will kill your ability to reproducibly and faithfully stain. You will likely see less positivity than you should (you might even miss positive staining altogether). For beta-gal/X-Gal staining, you should snap freeze the brains in OCT without fixation and fix with 0.2% glutaraldehyde after sectioning right before staining (this way you have more flexibility to do other things with your other brain sections like immunostain etc as GA destroys immunostaining) ... at least for mouse and human and morphology is quite good ......... OR you can perfuse with 0.2% glutaraldehyde followed by a 0.2% GA submersion fix and then sucrose etc etc. There is a whole protocol for this as you need EGTA and Mg etc in your fixing solutions. Let me know if you need more info. >Hi everybody, > >I had a question regarding fixing rat brain. We are currently >perfusing and fixing with a 4% para / 3% sucrose solution. I am >trying to get X-Gal staining, but I have read this may be >undetectable once tissue has been paraffin embedded. First off, has >anybody had luck with paraffinized tissue and x-gal? Secondly, we >wanted to snap freeze and section the tissue ourselves if paraffin >was not an option. From what I've tried thus far, I am not getting >the structural integrity that comes from paraffinzed tissue. I know >you are never going to get as good slides when frozen, but I think >it should look more intact than what I am seeing. The tissue looks >stretched out and very gappy. Could one of the reasons be that the >tissue is being overfixed? The tissue has been sitting in the >para/sucrose sol'n for anywhere from 3 days to 4 weeks. Maybe my >snap freezing technique is off, but I don't think ice >crystallization is the problem. Is there any advantage to using the >para/sucrose sol'n to fix, or is it just better to fix overnight, >move to sucrose gradient and then snap freeze? > >Thanks in advance for suggestions. > >-Eric -- From detmar <@t> mshri.on.ca Mon Aug 25 14:03:27 2008 From: detmar <@t> mshri.on.ca (Jacqui Detmar) Date: Mon Aug 25 14:03:41 2008 Subject: [Histonet] X-Gal Rat Brain In-Reply-To: References: <97F03AD4-CB3C-440B-A5B0-D374C0DFEFC1@mail.nih.gov> Message-ID: Hi Eric. I'm with Andrea here. I use a 0.2% glutaraldehyde solution, too. Paraformaldehyde will kill your enzyme; paraffin will kill it, too. I've tried paraffin-embedding, even with GA fixation and it was bad, very bad. Another option is, if you already know which section of the brain you're going for, is to make a slice where you want to see activity and do a whole-mount staining of GA-fixed tissue. Then, you can embed the tissue in paraffin, section it and counter-stain with nuclear fast red or Brazilin or whatever. I have done this with my mouse placental sections with very nice results...morphology is better than cryosections. But, you need to have an idea of which portion of the tissue you want to examine b/c the stain will only permeate the tissue several cell thicknesses. Just a thought, Jacqui Jacqui Detmar, Post-doctoral Fellow Samuel Lunenfeld Research Institute, Mount Sinai Hospital 25 Orde Street, room 6-1001 AJ, Toronto, ON, Canada M5T 3H7 phone: 416-586-4800 x5607 fax: 416-586-8588 email: detmar@mshri.on.ca From contact <@t> excaliburpathology.com Mon Aug 25 14:17:48 2008 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Mon Aug 25 14:17:57 2008 Subject: [Histonet] X-Gal rat brain Message-ID: <691706.63159.qm@web1108.biz.mail.sk1.yahoo.com> I have successfully done X-Gal on PFA fixed paraffin embedded specimens. The key is to not use xylene in processing or deparaffinization/clearing step. I used XS3 from StatLab, but probablt any substitute would work. Paula Pierce, BS, HTL(ASCP)HT Excalibur Pathology, Inc. 631 N Broadway Ave. Moore, OK 73160 405-759-3953 contact@excaliburpathology.com From pruegg <@t> ihctech.net Mon Aug 25 17:02:12 2008 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Aug 25 17:02:18 2008 Subject: [Histonet] muscle fibers Message-ID: <009301c906fe$3a73d7a0$6401a8c0@Patsyoffice> Are there muscle experts out there that can help with this project? "Do you have any leads on differentiating and staining muscle fiber types? We have mixed fiber type whole muscle embedded in paraffin and want to tell if there is a difference between rats in the % distribution of muscle types. They are sometimes called fast twitch and slow twitch muscles. Any leads you could offer would be appreciated." Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From juditw <@t> u.washington.edu Mon Aug 25 17:53:43 2008 From: juditw <@t> u.washington.edu (Judith L. Williams) Date: Mon Aug 25 17:53:48 2008 Subject: [Histonet] muscle fibers In-Reply-To: <009301c906fe$3a73d7a0$6401a8c0@Patsyoffice> Message-ID: Hi Patsy - the best one stop shop for info on muscle fiber is in Carson's book on Enzyme Histochemistry - CH13. It covers introduction on muscle fiber types, stains, and various techniques for identifying fast and slow twitch fibers. Judy at U Washington On Mon, 25 Aug 2008, Patsy Ruegg wrote: > Are there muscle experts out there that can help with this project? > > > > "Do you have any leads on differentiating and staining muscle fiber types? > We have mixed fiber type whole muscle embedded in paraffin and want to tell > if there is a difference between rats in the % distribution of muscle types. > They are sometimes called fast twitch and slow twitch muscles. Any leads > you could offer would be appreciated." > > > > Best regards, > > Patsy > > > > > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech, LLC > Fitzsimmons BioScience Park > 12635 Montview Blvd. Suite 215 > Aurora, CO 80010 > P-720-859-4060 > F-720-859-4110 > wk email pruegg@ihctech.net > web site www.ihctech.net > > > > > This email is confidential and intended solely for the use of the Person(s) > ('the intended recipient') to whom it was addressed. Any views or opinions > presented are solely those of the author. It may contain information that is > privileged & confidential within the meaning of applicable law. Accordingly > any dissemination, distribution, copying, or other use of this message, or > any of its contents, by any person other than the intended recipient may > constitute a breach of civil or criminal law and is strictly prohibited. If > you are NOT the intended recipient please contact the sender and dispose of > this e-mail as soon as possible. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Judith Williams, PhD, HT(ASCP) Research Scientist Department of Comparative Medicine University of Washington Seattle, WA 98195 From llewllew <@t> shaw.ca Mon Aug 25 18:19:19 2008 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Mon Aug 25 18:19:46 2008 Subject: [Histonet] muscle fibers References: <009301c906fe$3a73d7a0$6401a8c0@Patsyoffice> Message-ID: <81ACC4B9B5644D4B9D69302E522CD9D1@mv1c183c0d909c> Enzyme histochemistry is probably the best, ATPase with preincubation in various buffers to remove selected isoenzymes. This is found in many histochemistry texts. Other enzyme systems can be used as well. They all require fresh tissue and crysostat sections. Possibly there may be an antibody against the fibres, if so that may work. It would have to be tried by an IHC expert. If neither is usable, then measuring the circumference of the fibres may indicate what type they are. This was used decades ago to classify the fibres in human muscle biopsies, but I do not know how relevant it may be to rat muscle. In any case, a microscope calibrated for measurements is needed. That is not difficult, just tedious. A reticule is inserted in one eyepiece and the markings on it related to micrometers using an engraved slide. You would also need a reference for the sizes of paraffin processed (i.e. shrunken) muscle fibres. Bryan Llewellyn ----- Original Message ----- From: "Patsy Ruegg" To: "'Histonet'" Sent: Monday, August 25, 2008 3:02 PM Subject: [Histonet] muscle fibers > Are there muscle experts out there that can help with this project? > > > > "Do you have any leads on differentiating and staining muscle fiber types? > We have mixed fiber type whole muscle embedded in paraffin and want to > tell > if there is a difference between rats in the % distribution of muscle > types. > They are sometimes called fast twitch and slow twitch muscles. Any leads > you could offer would be appreciated." > > > > Best regards, > > Patsy > > > > > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech, LLC > Fitzsimmons BioScience Park > 12635 Montview Blvd. Suite 215 > Aurora, CO 80010 > P-720-859-4060 > F-720-859-4110 > wk email pruegg@ihctech.net > web site www.ihctech.net > > > > > This email is confidential and intended solely for the use of the > Person(s) > ('the intended recipient') to whom it was addressed. Any views or opinions > presented are solely those of the author. It may contain information that > is > privileged & confidential within the meaning of applicable law. > Accordingly > any dissemination, distribution, copying, or other use of this message, or > any of its contents, by any person other than the intended recipient may > constitute a breach of civil or criminal law and is strictly prohibited. > If > you are NOT the intended recipient please contact the sender and dispose > of > this e-mail as soon as possible. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Tue Aug 26 04:11:55 2008 From: louise.renton <@t> gmail.com (louise renton) Date: Tue Aug 26 04:11:59 2008 Subject: [Histonet] frozen haematoxylin - update Message-ID: Hi all, i'm posting some comparative pictures of tissues stained with Mayer's haematoxylin stored at room temp and the same soution frozen for 1 week frozen at -20 deg C. Let me know what your assessment is. best regards -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From louise.renton <@t> gmail.com Tue Aug 26 05:40:46 2008 From: louise.renton <@t> gmail.com (louise renton) Date: Tue Aug 26 05:40:49 2008 Subject: [Histonet] formalin follow up Message-ID: having problems posting the images, wil try again later. -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From kgreen <@t> hsh.org Tue Aug 26 07:00:10 2008 From: kgreen <@t> hsh.org (Green, Kathy) Date: Tue Aug 26 07:00:41 2008 Subject: [Histonet] question Message-ID: Dear Fellow Histotechs - Where do people get their HER 2 neu controls from? For testing purposes we bought them from Ventana, but they are too costly, does anyone know of anyplace else to purchase them? Thanks. Kathy Green, HT Manager Histology Laboratory Holy Spirit Hospital 503 N. 21st Street Camp Hill, PA 17011 kgreen@hsh.org (717) 763-2930 Blackberry: (717) 370-1726 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You From rjbuesa <@t> yahoo.com Tue Aug 26 07:05:58 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Aug 26 07:06:06 2008 Subject: [Histonet] question In-Reply-To: Message-ID: <159982.63128.qm@web65715.mail.ac4.yahoo.com> Either you use those provided by DAKO with their kit, or you get positive cases well documented and approved by your pathololgist. Ren? J. --- On Tue, 8/26/08, Green, Kathy wrote: From: Green, Kathy Subject: [Histonet] question To: histonet@lists.utsouthwestern.edu Date: Tuesday, August 26, 2008, 8:00 AM Dear Fellow Histotechs - Where do people get their HER 2 neu controls from? For testing purposes we bought them from Ventana, but they are too costly, does anyone know of anyplace else to purchase them? Thanks. Kathy Green, HT Manager Histology Laboratory Holy Spirit Hospital 503 N. 21st Street Camp Hill, PA 17011 kgreen@hsh.org (717) 763-2930 Blackberry: (717) 370-1726 Confidentiality Disclaimer: The information contained in this communication may be confidential, is intended for the use of the recipient named above, and may be legally privileged.If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication, or any of its contents, is strictly prohibited. If you received this communication in error, please resend this communication to the sender and delete the original message and any copy of it from your computer system. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Hooten <@t> medinst.com Tue Aug 26 12:40:27 2008 From: Hooten <@t> medinst.com (Scott Hooten) Date: Tue Aug 26 12:40:36 2008 Subject: [Histonet] H&E Staining for SPI-Chem Low Acid GMA Message-ID: <48B407CC.8FD5.00E5.0@medinst.com> Hello, I am working with SPI-Chem Low Acid GMA for LM. I am having some background staining issues. I am using the following staining procedure: 1. Gill's Hematoxylin for 30 minutes 2. Rinse in distilled water, three changes 10 dips each 3. Blue in Scott's Tap Water Substitute, 2 to 3 minutes 4. Rinse in distilled water, two changes 10 dips each 5. Stain in Eosin Y for 6 minutes 6. Rinse in two changes of 95% alcohol very quickly 7. Rinse in two changes of Abs. alcohol, allow sections to remain in the last Abs. for one minute The embedding medium is taking up the hematoxylin so my sections are dark purple and it seems like the tissue didn't take in much of the hematoxylin. If anyone has used this type of GMA and have some good suggestions for staining it with H&E, I would appreciate it. Thanks. Scott R. Hooten Histology Technician MED Institute 1 Geddes Way West Lafayette, IN 47906 765-464-0817 ext. 1115 From tdobersztyn <@t> chmca.org Tue Aug 26 12:49:40 2008 From: tdobersztyn <@t> chmca.org (tdobersztyn@chmca.org) Date: Tue Aug 26 12:50:14 2008 Subject: [Histonet] Re: SV40 In-Reply-To: <20080826174439.0B1581D784C@interceptor.chmca.org> Message-ID: Histonet! Where can I purchase positive control blocks/slides for SV40?? ANY and all help will be appreciated. Thanks! Theresa R Dobersztyn BA, HT (ASCP) Electron Microscopy/Histology Labs Senior Technologist Department of Pathology Akron Children's Hospital 1 Perkins Square Akron, Ohio 44308 330-543-8279 From RSRICHMOND <@t> aol.com Tue Aug 26 13:05:14 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Tue Aug 26 13:05:19 2008 Subject: [Histonet] Re: muscle fibers Message-ID: >>Do you have any leads on differentiating and staining muscle fiber types? We have mixed fiber type whole muscle embedded in paraffin and want to tell if there is a difference between rats in the % distribution of muscle types. They are sometimes called fast twitch and slow twitch muscles.<< You can't do any of the enzymatic fiber-type stains (such as myosin ATPase) on paraffin embedded tissues. I think that there are some immunohistochemical techniques that can be used for this purpose. PAS (without amylase) in my experience will sometimes stain fiber types in human muscle, but it doesn't work consistently. Human and rat fiber types do not exactly correspond to each other, and of course there's always the species specificity question with IHC techniques. Bob Richmond Samurai Pathologist Knoxville TN From laurie.colbert <@t> huntingtonhospital.com Tue Aug 26 13:08:32 2008 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Tue Aug 26 13:08:37 2008 Subject: [Histonet] Large Funnel Message-ID: <57BE698966D5C54EAE8612E8941D7683039AE5AA@EXCHANGE3.huntingtonhospital.com> Does anyone know where I can get a really large funnel (for pouring used paraffin into a waste bottle)? I need one with a diameter of about 11-12 inches. Laurie Colbert From Barry.R.Rittman <@t> uth.tmc.edu Tue Aug 26 13:13:04 2008 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Tue Aug 26 13:13:09 2008 Subject: [Histonet] Large Funnel In-Reply-To: <57BE698966D5C54EAE8612E8941D7683039AE5AA@EXCHANGE3.huntingtonhospital.com> References: <57BE698966D5C54EAE8612E8941D7683039AE5AA@EXCHANGE3.huntingtonhospital.com> Message-ID: Laurie Just make one out of a sheet of cardboard and then you can dispose of it instead of having to clean the plastic funnel. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Tuesday, August 26, 2008 1:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Large Funnel Does anyone know where I can get a really large funnel (for pouring used paraffin into a waste bottle)? I need one with a diameter of about 11-12 inches. Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lamarti2 <@t> gundluth.org Tue Aug 26 13:20:26 2008 From: lamarti2 <@t> gundluth.org (lamarti2@gundluth.org) Date: Tue Aug 26 13:20:36 2008 Subject: [Histonet] Training for gross In-Reply-To: <774F7DC732C89743BB070F1E87D402541A81A0@TNTRIEXEVS04.triadhospitals.net> Message-ID: Question.... under CAP guideline ANP. 11600 ....is "Processing" (vs "grossing/gross examination) considered a form of high complexity testing? The reason I ask is because under CAP guideline ANP.11610 the statement is - "If individuals other than a pathologist, etc., assist in 'GROSS EXAMINATIONS", do such individuals qualify as HIGH COMPLEXITY testing personnel under CLIA-88 regulations?" There is absolutely NO mention of qualifications for individuals performing tissue "PROCESSING". Lisa A. Martin, PA (ASCP) Gundersen Lutheran Medical Center "Caldwell, Lia" To Sent by: "Sharon.Davis-Devine" histonet-bounces@ lists.utsouthwest cc ern.edu histonet@lists.utsouthwestern.edu Subject RE: [Histonet] Training for gross 08/22/2008 04:54 PM Sharon, This came up during a recent CAP inspection of a lab we inspected in Maricopa so I have taken the liberty of copying the information from the CAP guidelines regarding this issue for you. The first question defines high complexity testing and the second question addresses requirements for individuals performing these tasks. Hope this helps, have a great weekend! ~Lia According to CAP guidelines ANP.11600 "Are all macroscopic tissue examinations performed by a pathologist or pathology resident, or under supervision of a qualified pathologist? Note: Two levels of complexity of macroscopic tissue examination are defined, as follows: 1. Processing is defined as a tissue examination limited to description, inking and cutting of the specimen (if applicable), and submission of the entire specimen to histology. Tissue processing can be performed accroding to standardized protocols. Processing is generally limited to small specimens (skin ellipses, small biopsies, curettings, etc.) and does not require knowledge of anatomy. 2. Grossing (or gross examination) is defined as a tissue examination requiring a greater exercise of judgement and a knowledge of anatomy. Dissection of the specimen and selection of tissue samples for submission to histology are generally required. The specimen description is not necessarily stadardized. ANP.11610 "If individuals other than a pathologist or pathology resident assist in gross examinations, do such individuals qualify as high complexity testing personnel under CLIA-88 regulations? NOTE: The laboratory director may delegate the dissection of specimens to non-pathologist individuals; these individuals must be qualified as high complexity testing personnel under CLIA-88 regulations. The minimum training/experience required of such personnel is: 1. An earned associate degree in a laboratory science or medical laboratory technology, obtained from an accredited institution, OR 2. Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education must include 24 semester hours of medical laboratory technology courses, OR 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. In addition, the individual must have laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, the CAHEA, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), OR at least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. In addition, the CLIA-88 regulations include exceptions for grandfathered individuals; these regulations (42CFR493.1489 and 1491) may be found at http://www.phppo.cdc.gov/clia/regs/subpart_m.aspx#493.1487 It is the responsibility of the laboratory director to determine whether an individual's education, training and experience satisfies the requirements of this checklist question. This checklist question applies only to laboratories subject to CLIA-88 Lia M. Caldwell HT (ASCP) Histology Supervisor Oro Valley Pathology Dept. phone: (520) 901-3914 www.Lia.Caldwell@TriadHospitals.com "be yourself - everyone else is already taken." -unknown ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sharon.Davis-Devine Sent: Fri 8/22/2008 10:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Training for gross For all of you Histonetters out there I have a question about training individuals to gross in small specimens. Can a person with a degree and being a Cytotechnologist, Medical Technologist or a Histotechnologist gross in small biopsy samples? And if they can, what kind of training is required and for how long? We are losing one of our PA's and are contemplating replacing that person with a person with a degree. Thanks for the info. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Tue Aug 26 13:39:02 2008 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue Aug 26 13:39:09 2008 Subject: [Histonet] Large Funnel In-Reply-To: <57BE698966D5C54EAE8612E8941D7683039AE5AA@EXCHANGE3.huntingtonhospital.com> Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835C52@LSRIEXCH1.lsmaster.lifespan.org> Several companies sell them. Fisher offers a heavey duty polyethylene funnel in sizes up to 343 mm (about 14 inches). This should be fine for paraffin, though I wouldn't use it for xylene or other hydrocarbon solvents since it is polyethylene. Fisher also offers a polypropylene funnel in sizes up to 270 mm (about 11 inches), which would be good for wax or solvents. From rjbuesa <@t> yahoo.com Tue Aug 26 13:39:59 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Aug 26 13:40:03 2008 Subject: [Histonet] Large Funnel In-Reply-To: <57BE698966D5C54EAE8612E8941D7683039AE5AA@EXCHANGE3.huntingtonhospital.com> Message-ID: <609272.6638.qm@web65711.mail.ac4.yahoo.com> Check an auto supply store (they use them for used oil and gasoline). Ren? J --- On Tue, 8/26/08, Laurie Colbert wrote: From: Laurie Colbert Subject: [Histonet] Large Funnel To: histonet@lists.utsouthwestern.edu Date: Tuesday, August 26, 2008, 2:08 PM Does anyone know where I can get a really large funnel (for pouring used paraffin into a waste bottle)? I need one with a diameter of about 11-12 inches. Laurie Colbert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Tue Aug 26 13:41:59 2008 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue Aug 26 13:42:03 2008 Subject: [Histonet] Large Funnel - oops! In-Reply-To: <57BE698966D5C54EAE8612E8941D7683039AE5AA@EXCHANGE3.huntingtonhospital.com> Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835C53@LSRIEXCH1.lsmaster.lifespan.org> Oops! What was I thinking of - either the polypropylene or polyethylene funnels would be fine for either paraffin or solvents. It is polystyrene that would not be recommended for organic solvents! > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Laurie Colbert > Sent: Tuesday, August 26, 2008 2:08 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Large Funnel > > Does anyone know where I can get a really large funnel (for pouring used > paraffin into a waste bottle)? I need one with a diameter of about > 11-12 inches. > > > > Laurie Colbert > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From rjbuesa <@t> yahoo.com Tue Aug 26 13:43:51 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Aug 26 13:43:57 2008 Subject: [Histonet] Training for gross In-Reply-To: Message-ID: <537607.56060.qm@web65704.mail.ac4.yahoo.com> Because tissue processing, in 98% of all our laboratories in general and in all the histopathology labs is done by tissue processors, automatically. Only some labs doing electron microscopy still process samples manually and if a machine can process tissue, it cannot be considered as a "high complexity" task. Ren? J. --- On Tue, 8/26/08, lamarti2@gundluth.org wrote: From: lamarti2@gundluth.org Subject: RE: [Histonet] Training for gross To: "Caldwell, Lia" Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu, "Sharon.Davis-Devine" Date: Tuesday, August 26, 2008, 2:20 PM Question.... under CAP guideline ANP. 11600 ....is "Processing" (vs "grossing/gross examination) considered a form of high complexity testing? The reason I ask is because under CAP guideline ANP.11610 the statement is - "If individuals other than a pathologist, etc., assist in 'GROSS EXAMINATIONS", do such individuals qualify as HIGH COMPLEXITY testing personnel under CLIA-88 regulations?" There is absolutely NO mention of qualifications for individuals performing tissue "PROCESSING". Lisa A. Martin, PA (ASCP) Gundersen Lutheran Medical Center "Caldwell, Lia" To Sent by: "Sharon.Davis-Devine" histonet-bounces@ lists.utsouthwest cc ern.edu histonet@lists.utsouthwestern.edu Subject RE: [Histonet] Training for gross 08/22/2008 04:54 PM Sharon, This came up during a recent CAP inspection of a lab we inspected in Maricopa so I have taken the liberty of copying the information from the CAP guidelines regarding this issue for you. The first question defines high complexity testing and the second question addresses requirements for individuals performing these tasks. Hope this helps, have a great weekend! ~Lia According to CAP guidelines ANP.11600 "Are all macroscopic tissue examinations performed by a pathologist or pathology resident, or under supervision of a qualified pathologist? Note: Two levels of complexity of macroscopic tissue examination are defined, as follows: 1. Processing is defined as a tissue examination limited to description, inking and cutting of the specimen (if applicable), and submission of the entire specimen to histology. Tissue processing can be performed accroding to standardized protocols. Processing is generally limited to small specimens (skin ellipses, small biopsies, curettings, etc.) and does not require knowledge of anatomy. 2. Grossing (or gross examination) is defined as a tissue examination requiring a greater exercise of judgement and a knowledge of anatomy. Dissection of the specimen and selection of tissue samples for submission to histology are generally required. The specimen description is not necessarily stadardized. ANP.11610 "If individuals other than a pathologist or pathology resident assist in gross examinations, do such individuals qualify as high complexity testing personnel under CLIA-88 regulations? NOTE: The laboratory director may delegate the dissection of specimens to non-pathologist individuals; these individuals must be qualified as high complexity testing personnel under CLIA-88 regulations. The minimum training/experience required of such personnel is: 1. An earned associate degree in a laboratory science or medical laboratory technology, obtained from an accredited institution, OR 2. Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education must include 24 semester hours of medical laboratory technology courses, OR 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. In addition, the individual must have laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, the CAHEA, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), OR at least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. In addition, the CLIA-88 regulations include exceptions for grandfathered individuals; these regulations (42CFR493.1489 and 1491) may be found at http://www.phppo.cdc.gov/clia/regs/subpart_m.aspx#493.1487 It is the responsibility of the laboratory director to determine whether an individual's education, training and experience satisfies the requirements of this checklist question. This checklist question applies only to laboratories subject to CLIA-88 Lia M. Caldwell HT (ASCP) Histology Supervisor Oro Valley Pathology Dept. phone: (520) 901-3914 www.Lia.Caldwell@TriadHospitals.com "be yourself - everyone else is already taken." -unknown ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sharon.Davis-Devine Sent: Fri 8/22/2008 10:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Training for gross For all of you Histonetters out there I have a question about training individuals to gross in small specimens. Can a person with a degree and being a Cytotechnologist, Medical Technologist or a Histotechnologist gross in small biopsy samples? And if they can, what kind of training is required and for how long? We are losing one of our PA's and are contemplating replacing that person with a person with a degree. Thanks for the info. Sharon Davis-Devine, CT (ASCP) Cytology Supervisor Carle Clinic 602 West University Urbana, Illinois 61801 Phone: 217-383-3572 Email: sharon.davis-devine@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jessica.Vacca <@t> HCAhealthcare.com Tue Aug 26 14:02:06 2008 From: Jessica.Vacca <@t> HCAhealthcare.com (Vacca Jessica) Date: Tue Aug 26 14:02:18 2008 Subject: [Histonet] Cytology sendout Message-ID: <41E16A15CE78374EA45B57E0F94339B80441172A@ORLEV01.hca.corpad.net> For those of you that DO NOT do cytology in-house- How do you answer the question for CYP.07690 Are 90% of reports on routine non-gynecologic cytology cases completed within 2 working days of receipt by the laboratory performing the evaluation? We send all non-gyn cyto's to a reference lab with 2 pick up's a day. 1230 and 1430. Usually we can get specimens back by the next day and to the Dr's to sign out by the 2 day TAT. But what if you are at the mercy of your reference lab? Do you make a clause for all specimens in the event that this is happening. From pjfnefro <@t> duke.edu Tue Aug 26 14:04:28 2008 From: pjfnefro <@t> duke.edu (Pat Flannery) Date: Tue Aug 26 14:04:37 2008 Subject: [Histonet] Large Funnel In-Reply-To: <609272.6638.qm@web65711.mail.ac4.yahoo.com> References: <609272.6638.qm@web65711.mail.ac4.yahoo.com> Message-ID: <4DF60702-71A9-4DB6-8BA6-25FE09F83064@duke.edu> That's an excellent idea. I got a set of 4 different sized funnels for 89 cents in an auto parts store and I use the biggest ones (with *really* big paper) to filter stains quickly. On Aug 26, 2008, at 2:39 PM, Rene J Buesa wrote: > Check an auto supply store (they use them for used oil and gasoline). > Ren? J > > --- On Tue, 8/26/08, Laurie Colbert > wrote: > > From: Laurie Colbert > Subject: [Histonet] Large Funnel > To: histonet@lists.utsouthwestern.edu > Date: Tuesday, August 26, 2008, 2:08 PM > > Does anyone know where I can get a really large funnel (for pouring > used > paraffin into a waste bottle)? I need one with a diameter of about > 11-12 inches. > > > > Laurie Colbert > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ajwj <@t> uiuc.edu Tue Aug 26 14:05:12 2008 From: ajwj <@t> uiuc.edu (Amy Wagoner Johnson) Date: Tue Aug 26 14:05:22 2008 Subject: [Histonet] mechanism of staining for Sanderson's with acid fuchsin counterstain Message-ID: <660309cd0808261205t5d2a59b2w90461a7f104770a1@mail.gmail.com> All, I would like to know the mechanism of staining for Sanderson's with acid fuchsin counterstain used with undecalcified PMMA embedded bone or bone/scaffold samples. For example, osmium tetroxide stains lipids. Thanks! -- Amy Wagoner Johnson, Ph.D. Assistant Professor Mechanical Science and Engineering University of Illinois at Urbana-Champaign From dlschneider <@t> gmail.com Tue Aug 26 14:35:53 2008 From: dlschneider <@t> gmail.com (Daniel Schneider) Date: Tue Aug 26 14:36:01 2008 Subject: [Histonet] Training for gross In-Reply-To: <537607.56060.qm@web65704.mail.ac4.yahoo.com> References: <537607.56060.qm@web65704.mail.ac4.yahoo.com> Message-ID: <1085e7000808261235r2c689e0bme4ce6b055f5a109b@mail.gmail.com> There's processing and then there's processing. "Processing" under the CAP guideline in question means grossing of small specimens that fall short of of the CAP definition of grossing. To paraphrase, they are small specimens that are entirely submitted, and of which a knowledge of anatomy is not required for their appropriate submission and description. I.e. most biopsies. Daniel Schneider On 8/26/08, Rene J Buesa wrote: > > Because tissue processing, in 98% of all our laboratories in general and in > all the histopathology labs is done by tissue processors, automatically. > Only some labs doing electron microscopy still process samples manually and > if a machine can process tissue, it cannot be considered as a "high > complexity" task. > Ren? J. > > --- On Tue, 8/26/08, lamarti2@gundluth.org wrote: > > From: lamarti2@gundluth.org > Subject: RE: [Histonet] Training for gross > To: "Caldwell, Lia" > Cc: histonet@lists.utsouthwestern.edu, > histonet-bounces@lists.utsouthwestern.edu, "Sharon.Davis-Devine" < > Sharon.Davis-Devine@carle.com> > Date: Tuesday, August 26, 2008, 2:20 PM > > Question.... under CAP guideline ANP. 11600 ....is "Processing" (vs > "grossing/gross examination) considered a form of high complexity > testing? > > The reason I ask is because under CAP guideline ANP.11610 the statement is > - "If individuals other than > a pathologist, etc., assist in 'GROSS EXAMINATIONS", do such > individuals > qualify as HIGH COMPLEXITY testing > personnel under CLIA-88 regulations?" There is absolutely NO mention of > qualifications for individuals performing > tissue "PROCESSING". > > > > Lisa A. Martin, PA (ASCP) > Gundersen Lutheran Medical Center > > > > > "Caldwell, Lia" > > adHospitals.com> To > Sent by: "Sharon.Davis-Devine" > > histonet-bounces@ > > lists.utsouthwest cc > ern.edu histonet@lists.utsouthwestern.edu > Subject > RE: [Histonet] Training for gross > 08/22/2008 04:54 > PM > > > > > > > > > Sharon, > This came up during a recent CAP inspection of a lab we inspected in > Maricopa so I have taken the liberty of copying the information from the > CAP guidelines regarding this issue for you. The first question defines > high complexity testing and the second question addresses requirements for > individuals performing these tasks. > Hope this helps, have a great weekend! > ~Lia > > > According to CAP guidelines > > ANP.11600 > > "Are all macroscopic tissue examinations performed by a pathologist or > pathology resident, or under supervision of a qualified pathologist? > > Note: Two levels of complexity of macroscopic tissue examination are > defined, as follows: > > 1. Processing is defined as a tissue examination limited to description, > inking and cutting of the specimen (if applicable), and submission of the > entire specimen to histology. Tissue processing can be performed accroding > to standardized protocols. Processing is generally limited to small > specimens (skin ellipses, small biopsies, curettings, etc.) and does not > require knowledge of anatomy. > > 2. Grossing (or gross examination) is defined as a tissue examination > requiring a greater exercise of judgement and a knowledge of anatomy. > Dissection of the specimen and selection of tissue samples for submission > to histology are generally required. The specimen description is not > necessarily stadardized. > > ANP.11610 > > "If individuals other than a pathologist or pathology resident assist in > gross examinations, do such individuals qualify as high complexity testing > personnel under CLIA-88 regulations? > > NOTE: The laboratory director may delegate the dissection of specimens to > non-pathologist individuals; these individuals must be qualified as high > complexity testing personnel under CLIA-88 regulations. The minimum > training/experience required of such personnel is: > > 1. An earned associate degree in a laboratory science or medical > laboratory technology, obtained from an accredited institution, OR > 2. Education/training equivalent to the above that includes at least 60 > semester hours or equivalent from an accredited institution. This > education must include 24 semester hours of medical laboratory technology > courses, OR 24 semester hours of science courses that includes 6 semester > hours of chemistry, 6 semester hours of biology, and 12 semester hours of > chemistry, biology or medical laboratory technology in any combination. In > addition, the individual must have laboratory training including either > completion of a clinical laboratory training program approved or accredited > by the ABHES, the CAHEA, or other organization approved by HHS (note that > this training may be included in the 60 semester hours listed above), OR at > least 3 months documented laboratory training in each specialty in which > the individual performs high complexity testing. > > In addition, the CLIA-88 regulations include exceptions for grandfathered > individuals; these regulations (42CFR493.1489 and 1491) may be found at > http://www.phppo.cdc.gov/clia/regs/subpart_m.aspx#493.1487 > > It is the responsibility of the laboratory director to determine whether an > individual's education, training and experience satisfies the requirements > of this checklist question. > > This checklist question applies only to laboratories subject to CLIA-88 > > > > Lia M. Caldwell HT (ASCP) > Histology Supervisor > Oro Valley Pathology Dept. > phone: (520) 901-3914 > www.Lia.Caldwell@TriadHospitals.com > "be yourself - everyone else is already taken." -unknown > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Sharon.Davis-Devine > Sent: Fri 8/22/2008 10:23 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Training for gross > > > > For all of you Histonetters out there I have a question about training > individuals to gross in small specimens. Can a person with a degree and > being a Cytotechnologist, Medical Technologist or a Histotechnologist > gross in small biopsy samples? And if they can, what kind of training > is required and for how long? We are losing one of our PA's and are > contemplating replacing that person with a person with a degree. Thanks > for the info. > > > > Sharon Davis-Devine, CT (ASCP) > > Cytology Supervisor > > Carle Clinic > > 602 West University > > Urbana, Illinois 61801 > > Phone: 217-383-3572 > > Email: sharon.davis-devine@carle.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It > is intended only for the use of the individual(s) and entity named > in the message. If you are not an intended recipient of this > message, please notify the sender immediately and delete the > material from your computer. Do not deliver, distribute or copy > this message and do not disclose its contents or take any action in > reliance on the information it contains. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ploykasek <@t> phenopath.com Tue Aug 26 14:48:57 2008 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue Aug 26 14:49:23 2008 Subject: [Histonet] Training for gross In-Reply-To: <537607.56060.qm@web65704.mail.ac4.yahoo.com> Message-ID: This question is an easy one to mis-read. By reading the note following the question you can figure out what CAP actually means. Here's the note: NOTE: Two levels of complexity of macroscopic tissue examination are defined, as follows: 1) Processing is defined as a tissue examination limited to description, inking and cutting of the specimen (if applicable), and submission of the entire specimen to histology. Tissue processing can be performed according to standardized protocols. Processing is generally limited to small specimens (skin ellipses, small biopsies, curettings, etc.) and does not require knowledge of anatomy. 2) Grossing (or gross examination) is defined as a tissue examination requiring a greater exercise of judgment and a knowledge of anatomy. Dissection of the specimen and selection of tissue samples for submission to histology are generally required. The specimen description is not necessarily standardized. In a latter question this is addressed: ANP.11665 Phase I Are there written procedures for processing specimens? NOTE: This question refers to processing as defined in ANP.11600, and applies only to processing of specimens by non-pathologist individuals who are not qualified as high complexity testing personnel under CLIA-88. I take this to mean that personnel who are not classified as high complexity personnel can perform processing of specimens as long as there are explicit SOPs. When I inspected a small lab last year where histotechs were processing? small, uncomplicated specimens, the documentation I required was that there were specific SOPs for each type of specimen they were grossing & that the pathologists had signed off on their competency assessment for these tasks. Patti Loykasek BS, HTL, QIHC Clinical IHC Supervisor PhenoPath Laboratories Seattle, WA > Because tissue processing, in 98% of all our laboratories in general and in > all the histopathology labs is done by tissue processors, automatically. Only > some labs doing electron microscopy still process samples manually and if a > machine can process tissue, it cannot be considered as a "high complexity" > task. > Ren? J. > > --- On Tue, 8/26/08, lamarti2@gundluth.org wrote: > > From: lamarti2@gundluth.org > Subject: RE: [Histonet] Training for gross > To: "Caldwell, Lia" > Cc: histonet@lists.utsouthwestern.edu, > histonet-bounces@lists.utsouthwestern.edu, "Sharon.Davis-Devine" > > Date: Tuesday, August 26, 2008, 2:20 PM > > Question.... under CAP guideline ANP. 11600 ....is "Processing" (vs > "grossing/gross examination) considered a form of high complexity > testing? > > The reason I ask is because under CAP guideline ANP.11610 the statement is > - "If individuals other than > a pathologist, etc., assist in 'GROSS EXAMINATIONS", do such > individuals > qualify as HIGH COMPLEXITY testing > personnel under CLIA-88 regulations?" There is absolutely NO mention of > qualifications for individuals performing > tissue "PROCESSING". > > > > Lisa A. Martin, PA (ASCP) > Gundersen Lutheran Medical Center > > > > > "Caldwell, Lia" > > adHospitals.com> To > Sent by: "Sharon.Davis-Devine" > > histonet-bounces@ > > lists.utsouthwest cc > ern.edu histonet@lists.utsouthwestern.edu > Subject > RE: [Histonet] Training for gross > 08/22/2008 04:54 > PM > > > > > > > > > Sharon, > This came up during a recent CAP inspection of a lab we inspected in > Maricopa so I have taken the liberty of copying the information from the > CAP guidelines regarding this issue for you. The first question defines > high complexity testing and the second question addresses requirements for > individuals performing these tasks. > Hope this helps, have a great weekend! > ~Lia > > > According to CAP guidelines > > ANP.11600 > > "Are all macroscopic tissue examinations performed by a pathologist or > pathology resident, or under supervision of a qualified pathologist? > > Note: Two levels of complexity of macroscopic tissue examination are > defined, as follows: > > 1. Processing is defined as a tissue examination limited to description, > inking and cutting of the specimen (if applicable), and submission of the > entire specimen to histology. Tissue processing can be performed accroding > to standardized protocols. Processing is generally limited to small > specimens (skin ellipses, small biopsies, curettings, etc.) and does not > require knowledge of anatomy. > > 2. Grossing (or gross examination) is defined as a tissue examination > requiring a greater exercise of judgement and a knowledge of anatomy. > Dissection of the specimen and selection of tissue samples for submission > to histology are generally required. The specimen description is not > necessarily stadardized. > > ANP.11610 > > "If individuals other than a pathologist or pathology resident assist in > gross examinations, do such individuals qualify as high complexity testing > personnel under CLIA-88 regulations? > > NOTE: The laboratory director may delegate the dissection of specimens to > non-pathologist individuals; these individuals must be qualified as high > complexity testing personnel under CLIA-88 regulations. The minimum > training/experience required of such personnel is: > > 1. An earned associate degree in a laboratory science or medical > laboratory technology, obtained from an accredited institution, OR > 2. Education/training equivalent to the above that includes at least 60 > semester hours or equivalent from an accredited institution. This > education must include 24 semester hours of medical laboratory technology > courses, OR 24 semester hours of science courses that includes 6 semester > hours of chemistry, 6 semester hours of biology, and 12 semester hours of > chemistry, biology or medical laboratory technology in any combination. In > addition, the individual must have laboratory training including either > completion of a clinical laboratory training program approved or accredited > by the ABHES, the CAHEA, or other organization approved by HHS (note that > this training may be included in the 60 semester hours listed above), OR at > least 3 months documented laboratory training in each specialty in which > the individual performs high complexity testing. > > In addition, the CLIA-88 regulations include exceptions for grandfathered > individuals; these regulations (42CFR493.1489 and 1491) may be found at > http://www.phppo.cdc.gov/clia/regs/subpart_m.aspx#493.1487 > > It is the responsibility of the laboratory director to determine whether an > individual's education, training and experience satisfies the requirements > of this checklist question. > > This checklist question applies only to laboratories subject to CLIA-88 > > > > Lia M. Caldwell HT (ASCP) > Histology Supervisor > Oro Valley Pathology Dept. > phone: (520) 901-3914 > www.Lia.Caldwell@TriadHospitals.com > "be yourself - everyone else is already taken." -unknown > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Sharon.Davis-Devine > Sent: Fri 8/22/2008 10:23 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Training for gross > > > > For all of you Histonetters out there I have a question about training > individuals to gross in small specimens. Can a person with a degree and > being a Cytotechnologist, Medical Technologist or a Histotechnologist > gross in small biopsy samples? And if they can, what kind of training > is required and for how long? We are losing one of our PA's and are > contemplating replacing that person with a person with a degree. Thanks > for the info. > > > > Sharon Davis-Devine, CT (ASCP) > > Cytology Supervisor > > Carle Clinic > > 602 West University > > Urbana, Illinois 61801 > > Phone: 217-383-3572 > > Email: sharon.davis-devine@carle.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It > is intended only for the use of the individual(s) and entity named > in the message. If you are not an intended recipient of this > message, please notify the sender immediately and delete the > material from your computer. Do not deliver, distribute or copy > this message and do not disclose its contents or take any action in > reliance on the information it contains. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From juditw <@t> u.washington.edu Tue Aug 26 17:18:33 2008 From: juditw <@t> u.washington.edu (Judith L. Williams) Date: Tue Aug 26 17:18:36 2008 Subject: [Histonet] Slide storage for -80C Message-ID: Hi Histonetters- we have a researcher who has a lot of slides in boxes that are 3.5" by 5.5" and they are looking for rack storage for the -80. I know previously on Histonet someone had the answer for the small black slide boxes, but does anyone know about rack or shelf storage for the larger boxes??? These are of course that funny in between size. Any help would be greatly appreciated- the tech do NOT want to spend days transfering slides to smaller boxes just to store them! Judy Judith Williams, PhD, HT(ASCP) Research Scientist Department of Comparative Medicine University of Washington Seattle, WA 98195 From jnocito <@t> satx.rr.com Wed Aug 27 05:51:28 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Aug 27 05:51:26 2008 Subject: [Histonet] Training for gross References: Message-ID: <7E171BB9D6644639B896FA097C5156B4@yourxhtr8hvc4p> This is why I can't stand CAP. For over 100 years, processing was understood as putting tissue samples through a dehydrant, clearing agent and paraffin. Now processing is the motion of putting tissue in a cassette without cutting it such as prostate bx's, embs, cervical bxs, etc. Next, they make all this hoopla about complexity testing. Please read the section in red about a 3 month training period to be able to gross. This crap has to stop. CAP is so ambiguous it's driving me crazy. Is it Friday yet?????? JTT ANP.11610 Phase II N/A YES NO If individuals other than a pathologist or pathology resident assist in gross examinations, do such individuals qualify as high complexity testing personnel under CLIA-88 regulations? NOTE: The laboratory director may delegate the dissection of specimens to non-pathologist individuals; these individuals must be qualified as high complexity testing personnel under CLIA-88 regulations. The minimum training/experience required of such personnel is: 1. An earned associate degree in a laboratory science or medical laboratory technology, obtained from an accredited institution, OR 2. Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education must include 24 semester hours of medical laboratory technology courses, OR 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. In addition, the individual must have laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, NAACLA, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), OR at least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. ----- Original Message ----- From: To: "Caldwell, Lia" Cc: ; ; "Sharon.Davis-Devine" Sent: Tuesday, August 26, 2008 1:20 PM Subject: RE: [Histonet] Training for gross > Question.... under CAP guideline ANP. 11600 ....is "Processing" (vs > "grossing/gross examination) considered a form of high complexity testing? > > The reason I ask is because under CAP guideline ANP.11610 the statement is > - "If individuals other than > a pathologist, etc., assist in 'GROSS EXAMINATIONS", do such individuals > qualify as HIGH COMPLEXITY testing > personnel under CLIA-88 regulations?" There is absolutely NO mention of > qualifications for individuals performing > tissue "PROCESSING". > > > > Lisa A. Martin, PA (ASCP) > Gundersen Lutheran Medical Center > > > > > "Caldwell, Lia" > adHospitals.com> To > Sent by: "Sharon.Davis-Devine" > histonet-bounces@ > lists.utsouthwest cc > ern.edu histonet@lists.utsouthwestern.edu > Subject > RE: [Histonet] Training for gross > 08/22/2008 04:54 > PM > > > > > > > > > Sharon, > This came up during a recent CAP inspection of a lab we inspected in > Maricopa so I have taken the liberty of copying the information from the > CAP guidelines regarding this issue for you. The first question defines > high complexity testing and the second question addresses requirements for > individuals performing these tasks. > Hope this helps, have a great weekend! > ~Lia > > > According to CAP guidelines > > ANP.11600 > > "Are all macroscopic tissue examinations performed by a pathologist or > pathology resident, or under supervision of a qualified pathologist? > > Note: Two levels of complexity of macroscopic tissue examination are > defined, as follows: > > 1. Processing is defined as a tissue examination limited to description, > inking and cutting of the specimen (if applicable), and submission of the > entire specimen to histology. Tissue processing can be performed accroding > to standardized protocols. Processing is generally limited to small > specimens (skin ellipses, small biopsies, curettings, etc.) and does not > require knowledge of anatomy. > > 2. Grossing (or gross examination) is defined as a tissue examination > requiring a greater exercise of judgement and a knowledge of anatomy. > Dissection of the specimen and selection of tissue samples for submission > to histology are generally required. The specimen description is not > necessarily stadardized. > > ANP.11610 > > "If individuals other than a pathologist or pathology resident assist in > gross examinations, do such individuals qualify as high complexity testing > personnel under CLIA-88 regulations? > > NOTE: The laboratory director may delegate the dissection of specimens to > non-pathologist individuals; these individuals must be qualified as high > complexity testing personnel under CLIA-88 regulations. The minimum > training/experience required of such personnel is: > > 1. An earned associate degree in a laboratory science or medical > laboratory technology, obtained from an accredited institution, OR > 2. Education/training equivalent to the above that includes at least 60 > semester hours or equivalent from an accredited institution. This > education must include 24 semester hours of medical laboratory technology > courses, OR 24 semester hours of science courses that includes 6 semester > hours of chemistry, 6 semester hours of biology, and 12 semester hours of > chemistry, biology or medical laboratory technology in any combination. In > addition, the individual must have laboratory training including either > completion of a clinical laboratory training program approved or accredited > by the ABHES, the CAHEA, or other organization approved by HHS (note that > this training may be included in the 60 semester hours listed above), OR at > least 3 months documented laboratory training in each specialty in which > the individual performs high complexity testing. > > In addition, the CLIA-88 regulations include exceptions for grandfathered > individuals; these regulations (42CFR493.1489 and 1491) may be found at > http://www.phppo.cdc.gov/clia/regs/subpart_m.aspx#493.1487 > > It is the responsibility of the laboratory director to determine whether an > individual's education, training and experience satisfies the requirements > of this checklist question. > > This checklist question applies only to laboratories subject to CLIA-88 > > > > Lia M. Caldwell HT (ASCP) > Histology Supervisor > Oro Valley Pathology Dept. > phone: (520) 901-3914 > www.Lia.Caldwell@TriadHospitals.com > "be yourself - everyone else is already taken." -unknown > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Sharon.Davis-Devine > Sent: Fri 8/22/2008 10:23 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Training for gross > > > > For all of you Histonetters out there I have a question about training > individuals to gross in small specimens. Can a person with a degree and > being a Cytotechnologist, Medical Technologist or a Histotechnologist > gross in small biopsy samples? And if they can, what kind of training > is required and for how long? We are losing one of our PA's and are > contemplating replacing that person with a person with a degree. Thanks > for the info. > > > > Sharon Davis-Devine, CT (ASCP) > > Cytology Supervisor > > Carle Clinic > > 602 West University > > Urbana, Illinois 61801 > > Phone: 217-383-3572 > > Email: sharon.davis-devine@carle.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It > is intended only for the use of the individual(s) and entity named > in the message. If you are not an intended recipient of this > message, please notify the sender immediately and delete the > material from your computer. Do not deliver, distribute or copy > this message and do not disclose its contents or take any action in > reliance on the information it contains. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Wed Aug 27 05:52:31 2008 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Aug 27 05:52:18 2008 Subject: [Histonet] Cytology sendout References: <41E16A15CE78374EA45B57E0F94339B80441172A@ORLEV01.hca.corpad.net> Message-ID: mark the question as N/A. Since you are not performing the test, you have no control over the TAT. JTT ----- Original Message ----- From: "Vacca Jessica" To: Sent: Tuesday, August 26, 2008 2:02 PM Subject: [Histonet] Cytology sendout For those of you that DO NOT do cytology in-house- How do you answer the question for CYP.07690 Are 90% of reports on routine non-gynecologic cytology cases completed within 2 working days of receipt by the laboratory performing the evaluation? We send all non-gyn cyto's to a reference lab with 2 pick up's a day. 1230 and 1430. Usually we can get specimens back by the next day and to the Dr's to sign out by the 2 day TAT. But what if you are at the mercy of your reference lab? Do you make a clause for all specimens in the event that this is happening. -------------------------------------------------------------------------------- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mikael.niku <@t> helsinki.fi Wed Aug 27 06:52:18 2008 From: mikael.niku <@t> helsinki.fi (Mikael Niku) Date: Wed Aug 27 06:52:33 2008 Subject: [Histonet] (para)formaldehyde (was: in situ question) In-Reply-To: References: <006e01c9036a$e2abb3d0$97a5d680@mmkem12636> Message-ID: <002801c9083b$5b9c6a80$97a5d680@mmkem12636> Hello again! I don't have ANY scientific proof whatsoever of the difference between 10% formalin and 4% formaldehyde made from PFA. It's just something I think I have noticed in the lab, clearly enough to make me wonder. But you are right that the fixation protocols were never specifically controlled to allow real comparison, so maybe it indeed was some other factor. I grew in a developmental biology group and first learnt to use "PFA", and eventually started to wonder why some use it and some use formalin (often being the same person, for different applications!). Maybe I should really investigate it some day. With best regards, Mikael ----------------------------------------------------- Mikael Niku, PhD, university lecturer University of Helsinki, Division of Nutrition URL: mikael.nikunnakki.info - What do I think of western civilization? I think it would be a good idea! Gandhi ----------------------------------------------------- -----Original Message----- From: Tony Henwood [mailto:AnthonyH@chw.edu.au] Sent: 22. elokuuta 2008 2:17 To: Mikael Niku; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (para)formaldehyde (was: in situ question) Why would it be different? I am intrigued as to why IPX and ISH would reaxct differently in tissues fixed in either. Do you have any references? Or please write up your results and publish them (J Histotechnology or Biotechnic & Histochem are definitely worth considering). Often there will be differences but my experience indicates that it is often due to the fixation time. 10% buffered formalin is usually used at room temp, overnight at least, where as researchers for some reason often use 4% formaldehyde (made from polyformaldehyde) at 4oC for only a few hours at best. So often fixation occurs in the ethanols in processing. For valid comparisons fixation conditions must be standardised. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 From b-frederick <@t> northwestern.edu Wed Aug 27 07:39:22 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Aug 27 07:39:38 2008 Subject: [Histonet] (para)formaldehyde (was: in situ question) In-Reply-To: <002801c9083b$5b9c6a80$97a5d680@mmkem12636> Message-ID: <000d01c90841$f2235cb0$d00f7ca5@lurie.northwestern.edu> We receive tissue from researchers that was fixed in PFA. It goes into 10% NBF once it hits the processor. Sort of becomes s moot point for us. A lot of times it's a result of reading a VERY old paper or method (or so we've discovered)and they don't know any better. Post-docs are not techs and never will be. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility Histology supervisor ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mikael Niku Sent: Wednesday, August 27, 2008 6:52 AM To: 'Tony Henwood'; histonet@lists.utsouthwestern.edu; 'Geoff McAuliffe' Subject: RE: [Histonet] (para)formaldehyde (was: in situ question) Hello again! I don't have ANY scientific proof whatsoever of the difference between 10% formalin and 4% formaldehyde made from PFA. It's just something I think I have noticed in the lab, clearly enough to make me wonder. But you are right that the fixation protocols were never specifically controlled to allow real comparison, so maybe it indeed was some other factor. I grew in a developmental biology group and first learnt to use "PFA", and eventually started to wonder why some use it and some use formalin (often being the same person, for different applications!). Maybe I should really investigate it some day. With best regards, Mikael ----------------------------------------------------- Mikael Niku, PhD, university lecturer University of Helsinki, Division of Nutrition URL: mikael.nikunnakki.info - What do I think of western civilization? I think it would be a good idea! Gandhi ----------------------------------------------------- -----Original Message----- From: Tony Henwood [mailto:AnthonyH@chw.edu.au] Sent: 22. elokuuta 2008 2:17 To: Mikael Niku; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (para)formaldehyde (was: in situ question) Why would it be different? I am intrigued as to why IPX and ISH would reaxct differently in tissues fixed in either. Do you have any references? Or please write up your results and publish them (J Histotechnology or Biotechnic & Histochem are definitely worth considering). Often there will be differences but my experience indicates that it is often due to the fixation time. 10% buffered formalin is usually used at room temp, overnight at least, where as researchers for some reason often use 4% formaldehyde (made from polyformaldehyde) at 4oC for only a few hours at best. So often fixation occurs in the ethanols in processing. For valid comparisons fixation conditions must be standardised. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Aug 27 08:53:37 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Aug 27 08:53:43 2008 Subject: [Histonet] (para)formaldehyde (was: in situ question) In-Reply-To: <000d01c90841$f2235cb0$d00f7ca5@lurie.northwestern.edu> Message-ID: <70095.61440.qm@web65715.mail.ac4.yahoo.com> Very good everything you said EXCEPT for "Post-doc are not tech and never will be" because anybody and everybody that is willing to learn and pay attention to what they do CAN and WILL become a good tech. Being a tech is NOT a genetic trait within the great scheme of evolution, you know? Ren? J. --- On Wed, 8/27/08, Bernice Frederick wrote: From: Bernice Frederick Subject: RE: [Histonet] (para)formaldehyde (was: in situ question) To: "'Mikael Niku'" , "'Tony Henwood'" , histonet@lists.utsouthwestern.edu, "'Geoff McAuliffe'" Date: Wednesday, August 27, 2008, 8:39 AM We receive tissue from researchers that was fixed in PFA. It goes into 10% NBF once it hits the processor. Sort of becomes s moot point for us. A lot of times it's a result of reading a VERY old paper or method (or so we've discovered)and they don't know any better. Post-docs are not techs and never will be. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility Histology supervisor ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 From JWeems <@t> sjha.org Wed Aug 27 08:59:36 2008 From: JWeems <@t> sjha.org (Weems, Joyce) Date: Wed Aug 27 08:59:55 2008 Subject: [Histonet] (para)formaldehyde (was: in situ question) In-Reply-To: <70095.61440.qm@web65715.mail.ac4.yahoo.com> References: <000d01c90841$f2235cb0$d00f7ca5@lurie.northwestern.edu> <70095.61440.qm@web65715.mail.ac4.yahoo.com> Message-ID: <982A0A9461F9BF438C7B19A6E425A383553F3D@ITSSSXM01V6.one.ads.che.org> Oh but it is... I'm still evolving and hoping I'll be a good one when I grow up! J:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, August 27, 2008 9:54 AM To: 'Mikael Niku'; 'Tony Henwood'; histonet@lists.utsouthwestern.edu; 'Geoff McAuliffe'; Bernice Frederick Subject: RE: [Histonet] (para)formaldehyde (was: in situ question) Very good everything you said EXCEPT for "Post-doc are not tech and never will be" because anybody and everybody that is willing to learn and pay attention to what they do CAN and WILL become a good tech. Being a tech is NOT a genetic trait within the great scheme of evolution, you know? Ren? J. --- On Wed, 8/27/08, Bernice Frederick wrote: From: Bernice Frederick Subject: RE: [Histonet] (para)formaldehyde (was: in situ question) To: "'Mikael Niku'" , "'Tony Henwood'" , histonet@lists.utsouthwestern.edu, "'Geoff McAuliffe'" Date: Wednesday, August 27, 2008, 8:39 AM We receive tissue from researchers that was fixed in PFA. It goes into 10% NBF once it hits the processor. Sort of becomes s moot point for us. A lot of times it's a result of reading a VERY old paper or method (or so we've discovered)and they don't know any better. Post-docs are not techs and never will be. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility Histology supervisor ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From SBarnes <@t> elch.org Wed Aug 27 09:04:26 2008 From: SBarnes <@t> elch.org (Sue Barnes) Date: Wed Aug 27 09:04:16 2008 Subject: [Histonet] Cytology sendout In-Reply-To: <41E16A15CE78374EA45B57E0F94339B80441172A@ORLEV01.hca.corpad.net> Message-ID: <807C106E9A171C46B0CF253C2507971E15AC70@elchex01.elch.net> We would answer it as NA, we don't do gyn cytology in house and this is how we answered each tme. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Vacca Jessica Sent: Tuesday, August 26, 2008 3:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cytology sendout For those of you that DO NOT do cytology in-house- How do you answer the question for CYP.07690 Are 90% of reports on routine non-gynecologic cytology cases completed within 2 working days of receipt by the laboratory performing the evaluation? We send all non-gyn cyto's to a reference lab with 2 pick up's a day. 1230 and 1430. Usually we can get specimens back by the next day and to the Dr's to sign out by the 2 day TAT. But what if you are at the mercy of your reference lab? Do you make a clause for all specimens in the event that this is happening. From rjbuesa <@t> yahoo.com Wed Aug 27 09:10:06 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Aug 27 09:10:14 2008 Subject: [Histonet] (para)formaldehyde (was: in situ question) In-Reply-To: <982A0A9461F9BF438C7B19A6E425A383553F3D@ITSSSXM01V6.one.ads.che.org> Message-ID: <72242.5179.qm@web65701.mail.ac4.yahoo.com> And you will get there with the required effort! --- On Wed, 8/27/08, Weems, Joyce wrote: From: Weems, Joyce Subject: RE: [Histonet] (para)formaldehyde (was: in situ question) To: rjbuesa@yahoo.com, "Mikael Niku" , "Tony Henwood" , histonet@lists.utsouthwestern.edu, "Geoff McAuliffe" , "Bernice Frederick" Date: Wednesday, August 27, 2008, 9:59 AM Oh but it is... I'm still evolving and hoping I'll be a good one when I grow up! J:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, August 27, 2008 9:54 AM To: 'Mikael Niku'; 'Tony Henwood'; histonet@lists.utsouthwestern.edu; 'Geoff McAuliffe'; Bernice Frederick Subject: RE: [Histonet] (para)formaldehyde (was: in situ question) Very good everything you said EXCEPT for "Post-doc are not tech and never will be" because anybody and everybody that is willing to learn and pay attention to what they do CAN and WILL become a good tech. Being a tech is NOT a genetic trait within the great scheme of evolution, you know? Ren? J. --- On Wed, 8/27/08, Bernice Frederick wrote: From: Bernice Frederick Subject: RE: [Histonet] (para)formaldehyde (was: in situ question) To: "'Mikael Niku'" , "'Tony Henwood'" , histonet@lists.utsouthwestern.edu, "'Geoff McAuliffe'" Date: Wednesday, August 27, 2008, 8:39 AM We receive tissue from researchers that was fixed in PFA. It goes into 10% NBF once it hits the processor. Sort of becomes s moot point for us. A lot of times it's a result of reading a VERY old paper or method (or so we've discovered)and they don't know any better. Post-docs are not techs and never will be. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility Histology supervisor ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. From carl.hobbs <@t> kcl.ac.uk Wed Aug 27 09:14:43 2008 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Wed Aug 27 09:15:17 2008 Subject: [Histonet] Re: H&E Staining for SPI-Chem Low Acid GMA Message-ID: <11D9615B89C10747B1C985966A63D7CA25A5884390@KCL-MAIL04.kclad.ds.kcl.ac.uk> Hi Scott, GMA resin will take up Haemalum......you will need to differentiate the Gills in acid. After Gill's staining: 1. Rinse in gently running tap water until clear. 2. Give slide say, 5 dips, in 0.5% HCL in distilled water, wash in tapwater 5mins ( I assume that your tapwater is slightly alkali - if not, then follow with Scott's) Examine microscopically. You will thus see if more differentiation in HCL is reqd or not. 3. Once you have optimally stained nuclei, counterstain in Eosin. 4. Wash in gently running tap water until excess eosin is removed and you are happy with balance ( this is slow, relative to alcohol, with no danger of sections becoming wrinkled because of the alcohol softening it.) 5. Carefully/firmly blot sections dry. I now leave them in a 60C oven ( only cos that's all I have) for 60 mins, minimum. 6. Place into xylene. Leave for 10 mins before mounting . A nice easy, reliable method, imho. NB: after cutting/mounting sections, leave in 60C oven for 2 hrs to ensure that they are "baked" on. Carl From Barry.R.Rittman <@t> uth.tmc.edu Wed Aug 27 09:23:40 2008 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Wed Aug 27 09:23:54 2008 Subject: [Histonet] (para)formaldehyde (was: in situ question) In-Reply-To: <70095.61440.qm@web65715.mail.ac4.yahoo.com> References: <000d01c90841$f2235cb0$d00f7ca5@lurie.northwestern.edu> <70095.61440.qm@web65715.mail.ac4.yahoo.com> Message-ID: While it is true that most people are "trainable" most post docs generally do not have the time to put in to become proficient, and in any case we may not have the time to train them. When they learn about lab techniques it is generally only from the point of view of "what is the minimum that I need to know as I only have a very limited time to do this". Histotechs in general are continually learning outside this narrow box. Without this attitude work would become a never ending tedious task. I think that what most people do when teaching post docs the basics of tissue preparation is give them samples that will always work. This gives them a false impression and they will often believe that histology is very easy. It is only if given some of the challenging material that histotechs have to cut that they will get a true appreciation of the level of skill involved. While may pathologists have a very good idea of the processes involved in turning out sections, some do not and share the same opinion that many post docs often have. If I had my choice I would make it mandatory for all post docs and pathologist to spend 6 months in a lab in order to experience the range of problems that can occur. I have found that those post docs and pathologists that have some experience in a histo lab are much more considerate of the work carried out by histotechs and more realistic with their requests. My opinion. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, August 27, 2008 8:54 AM To: 'Mikael Niku'; 'Tony Henwood'; histonet@lists.utsouthwestern.edu; 'Geoff McAuliffe'; Bernice Frederick Subject: RE: [Histonet] (para)formaldehyde (was: in situ question) Very good everything you said EXCEPT for "Post-doc are not tech and never will be" because anybody and everybody that is willing to learn and pay attention to what they do CAN and WILL become a good tech. Being a tech is NOT a genetic trait within the great scheme of evolution, you know? Ren? J. --- On Wed, 8/27/08, Bernice Frederick wrote: From: Bernice Frederick Subject: RE: [Histonet] (para)formaldehyde (was: in situ question) To: "'Mikael Niku'" , "'Tony Henwood'" , histonet@lists.utsouthwestern.edu, "'Geoff McAuliffe'" Date: Wednesday, August 27, 2008, 8:39 AM We receive tissue from researchers that was fixed in PFA. It goes into 10% NBF once it hits the processor. Sort of becomes s moot point for us. A lot of times it's a result of reading a VERY old paper or method (or so we've discovered)and they don't know any better. Post-docs are not techs and never will be. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility Histology supervisor ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Wed Aug 27 09:36:25 2008 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Wed Aug 27 09:36:40 2008 Subject: [Histonet] (para)formaldehyde (was: in situ question) In-Reply-To: Message-ID: <001601c90852$4bfdbb30$d00f7ca5@lurie.northwestern.edu> Well said. Now if we could have the post-docs here at NU read and understand.......A lot of our PI's are just as bad. I have issues when we get a new post-doc from a lab and no one tells them anything about the project they are taking over. We usually have to tell them. Just ask how they want it oriented or grossed and see the blank look on their faces. I would think there should be some science background somewhere. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rittman, Barry R Sent: Wednesday, August 27, 2008 9:24 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (para)formaldehyde (was: in situ question) While it is true that most people are "trainable" most post docs generally do not have the time to put in to become proficient, and in any case we may not have the time to train them. When they learn about lab techniques it is generally only from the point of view of "what is the minimum that I need to know as I only have a very limited time to do this". Histotechs in general are continually learning outside this narrow box. Without this attitude work would become a never ending tedious task. I think that what most people do when teaching post docs the basics of tissue preparation is give them samples that will always work. This gives them a false impression and they will often believe that histology is very easy. It is only if given some of the challenging material that histotechs have to cut that they will get a true appreciation of the level of skill involved. While may pathologists have a very good idea of the processes involved in turning out sections, some do not and share the same opinion that many post docs often have. If I had my choice I would make it mandatory for all post docs and pathologist to spend 6 months in a lab in order to experience the range of problems that can occur. I have found that those post docs and pathologists that have some experience in a histo lab are much more considerate of the work carried out by histotechs and more realistic with their requests. My opinion. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, August 27, 2008 8:54 AM To: 'Mikael Niku'; 'Tony Henwood'; histonet@lists.utsouthwestern.edu; 'Geoff McAuliffe'; Bernice Frederick Subject: RE: [Histonet] (para)formaldehyde (was: in situ question) Very good everything you said EXCEPT for "Post-doc are not tech and never will be" because anybody and everybody that is willing to learn and pay attention to what they do CAN and WILL become a good tech. Being a tech is NOT a genetic trait within the great scheme of evolution, you know? Ren? J. --- On Wed, 8/27/08, Bernice Frederick wrote: From: Bernice Frederick Subject: RE: [Histonet] (para)formaldehyde (was: in situ question) To: "'Mikael Niku'" , "'Tony Henwood'" , histonet@lists.utsouthwestern.edu, "'Geoff McAuliffe'" Date: Wednesday, August 27, 2008, 8:39 AM We receive tissue from researchers that was fixed in PFA. It goes into 10% NBF once it hits the processor. Sort of becomes s moot point for us. A lot of times it's a result of reading a VERY old paper or method (or so we've discovered)and they don't know any better. Post-docs are not techs and never will be. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility Histology supervisor ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kgrobert <@t> rci.rutgers.edu Wed Aug 27 09:59:44 2008 From: kgrobert <@t> rci.rutgers.edu (Kathleen Roberts) Date: Wed Aug 27 09:49:12 2008 Subject: [Histonet] (para)formaldehyde In-Reply-To: References: <000d01c90841$f2235cb0$d00f7ca5@lurie.northwestern.edu> <70095.61440.qm@web65715.mail.ac4.yahoo.com> Message-ID: <48B56BE0.6030708@rci.rutgers.edu> This is exactly the tack I take with the graduate students who take our Toxicological Pathology course, except I don't have the luxury of 6 months to teach them. They put to use the theory they are taught in the class in their lab project, where they gross, fix, process (in a machine),embed, cut and stain animal tissues-then they are to read the slides and attempt to diagnose which toxicant the animals were given. I warn them that I make it look VERY easy and that the learning curve for microtomy is high and requires time and lots of patience. I make sure they have difficult tissues (though I will start them out with easier ones in the beginning), and they are taught about artifacts and how to prevent them. They all walk away with a lot of appreciation for histology and what I do for a living. They may never do it again, or pay my lab to do histo for them as part of their research, but they know what's involved, at least. -Kathleen Roberts Rutgers University Rittman, Barry R wrote: >While it is true that most people are "trainable" most post docs generally do not have the time to put in to become proficient, and in any case we may not have the time to train them. When they learn about lab techniques it is generally only from the point of view of "what is the minimum that I need to know as I only have a very limited time to do this". >Histotechs in general are continually learning outside this narrow box. Without this attitude work would become a never ending tedious task. > >I think that what most people do when teaching post docs the basics of tissue preparation is give them samples that will always work. This gives them a false impression and they will often believe that histology is very easy. It is only if given some of the challenging material that histotechs have to cut that they will get a true appreciation of the level of skill involved. >While may pathologists have a very good idea of the processes involved in turning out sections, some do not and share the same opinion that many post docs often have. >If I had my choice I would make it mandatory for all post docs and pathologist to spend 6 months in a lab in order to experience the range of problems that can occur. I have found that those post docs and pathologists that have some experience in a histo lab are much more considerate of the work carried out by histotechs and more realistic with their requests. >My opinion. >Barry > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa >Sent: Wednesday, August 27, 2008 8:54 AM >To: 'Mikael Niku'; 'Tony Henwood'; histonet@lists.utsouthwestern.edu; 'Geoff McAuliffe'; Bernice Frederick >Subject: RE: [Histonet] (para)formaldehyde (was: in situ question) > >Very good everything you said EXCEPT for "Post-doc are not tech and never will be" because anybody and everybody that is willing to learn and pay attention to what they do CAN and WILL become a good tech. >Being a tech is NOT a genetic trait within the great scheme of evolution, you know? >Ren? J. > >--- On Wed, 8/27/08, Bernice Frederick wrote: > >From: Bernice Frederick >Subject: RE: [Histonet] (para)formaldehyde (was: in situ question) >To: "'Mikael Niku'" , "'Tony Henwood'" , histonet@lists.utsouthwestern.edu, "'Geoff McAuliffe'" >Date: Wednesday, August 27, 2008, 8:39 AM > >We receive tissue from researchers that was fixed in PFA. It goes into 10% >NBF once it hits the processor. Sort of becomes s moot point for us. A lot >of times it's a result of reading a VERY old paper or method (or so >we've >discovered)and they don't know any better. Post-docs are not techs and >never >will be. >Bernice > > >Bernice Frederick HTL (ASCP) >Northwestern University >Pathology Core Facility >Histology supervisor >ECOGPCO-RL >710 N Fairbanks Court >Olson 8-421 >Chicago,IL 60611 >312-503-3723 > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Sharon.Genest <@t> saskatoonhealthregion.ca Wed Aug 27 09:51:52 2008 From: Sharon.Genest <@t> saskatoonhealthregion.ca (Genest, Sharon SktnHR) Date: Wed Aug 27 10:08:35 2008 Subject: [Histonet] lean Message-ID: Has anyone done a lean process in their Histology Lab and are now on the maintenance side of the process? Can you share with me some changes in Your process? Did you purchase new equipment like slide or cassette printers rapid processing and embedding units? Sharon From rjbuesa <@t> yahoo.com Wed Aug 27 10:09:12 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Aug 27 10:09:21 2008 Subject: [Histonet] (para)formaldehyde (was: in situ question) In-Reply-To: Message-ID: <471301.95179.qm@web65709.mail.ac4.yahoo.com> In the hospital where I used to work, the pathology residents had to rotate through histology and they were given tough tasks, otherwise the failure is in the teacher and not in the student. Ren? J. --- On Wed, 8/27/08, Rittman, Barry R wrote: From: Rittman, Barry R Subject: RE: [Histonet] (para)formaldehyde (was: in situ question) To: histonet@lists.utsouthwestern.edu Date: Wednesday, August 27, 2008, 10:23 AM While it is true that most people are "trainable" most post docs generally do not have the time to put in to become proficient, and in any case we may not have the time to train them. When they learn about lab techniques it is generally only from the point of view of "what is the minimum that I need to know as I only have a very limited time to do this". Histotechs in general are continually learning outside this narrow box. Without this attitude work would become a never ending tedious task. I think that what most people do when teaching post docs the basics of tissue preparation is give them samples that will always work. This gives them a false impression and they will often believe that histology is very easy. It is only if given some of the challenging material that histotechs have to cut that they will get a true appreciation of the level of skill involved. While may pathologists have a very good idea of the processes involved in turning out sections, some do not and share the same opinion that many post docs often have. If I had my choice I would make it mandatory for all post docs and pathologist to spend 6 months in a lab in order to experience the range of problems that can occur. I have found that those post docs and pathologists that have some experience in a histo lab are much more considerate of the work carried out by histotechs and more realistic with their requests. My opinion. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, August 27, 2008 8:54 AM To: 'Mikael Niku'; 'Tony Henwood'; histonet@lists.utsouthwestern.edu; 'Geoff McAuliffe'; Bernice Frederick Subject: RE: [Histonet] (para)formaldehyde (was: in situ question) Very good everything you said EXCEPT for "Post-doc are not tech and never will be" because anybody and everybody that is willing to learn and pay attention to what they do CAN and WILL become a good tech. Being a tech is NOT a genetic trait within the great scheme of evolution, you know? Ren? J. --- On Wed, 8/27/08, Bernice Frederick wrote: From: Bernice Frederick Subject: RE: [Histonet] (para)formaldehyde (was: in situ question) To: "'Mikael Niku'" , "'Tony Henwood'" , histonet@lists.utsouthwestern.edu, "'Geoff McAuliffe'" Date: Wednesday, August 27, 2008, 8:39 AM We receive tissue from researchers that was fixed in PFA. It goes into 10% NBF once it hits the processor. Sort of becomes s moot point for us. A lot of times it's a result of reading a VERY old paper or method (or so we've discovered)and they don't know any better. Post-docs are not techs and never will be. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility Histology supervisor ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Wed Aug 27 14:05:55 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Wed Aug 27 14:06:04 2008 Subject: [Histonet] Re: H&E Staining for SPI-Chem Low Acid GMA References: <11D9615B89C10747B1C985966A63D7CA25A5884390@KCL-MAIL04.kclad.ds.kcl.ac.uk> Message-ID: <001101c90877$ee858cf0$6501a8c0@Sunney> We never had much plastic background staining with Gill 3 hematoxylin on thin GMA tissue sections e.g. 1 to 3 um, and the staining time was never 30 minutes. Even a thicker GMA section was stained no more than 10 minutes, rinsed and blued with Scotts tap water, air dried then stained with eosin phloxine. We never had to differentiate our hematoxylin with any kind of acid HCL or acetic acid clarifier. Your section will probably be totally stained within 5 minutes, even with GMA media. We never used clarifier but the slight blue imparted to the plastic never was an factor for viewing or photography. If your section is in the very thin thickness range, 30 minutes staining time is NOT going to make the nuclei bluer as there is just not enough tissue there to stain in the first place. At that thickness you are cutting through the nuclei. The nuclei look more like nuclei in EPON sections cut very thin (0.5 to 1 um thick). HOwever, the longer the PLASTIC is in the stain, the more likely this will pick the hematoxylin and cause unsightly background. We found eosin phloxine gave better tinctorial quality to the sections than just eosin y. Gayle M. Callis' HTL,HT,MT(ASCP) ----- Original Message ----- From: "Hobbs, Carl" To: Sent: Wednesday, August 27, 2008 8:14 AM Subject: [Histonet] Re: H&E Staining for SPI-Chem Low Acid GMA Hi Scott, GMA resin will take up Haemalum......you will need to differentiate the Gills in acid. After Gill's staining: 1. Rinse in gently running tap water until clear. 2. Give slide say, 5 dips, in 0.5% HCL in distilled water, wash in tapwater 5mins ( I assume that your tapwater is slightly alkali - if not, then follow with Scott's) Examine microscopically. You will thus see if more differentiation in HCL is reqd or not. 3. Once you have optimally stained nuclei, counterstain in Eosin. 4. Wash in gently running tap water until excess eosin is removed and you are happy with balance ( this is slow, relative to alcohol, with no danger of sections becoming wrinkled because of the alcohol softening it.) 5. Carefully/firmly blot sections dry. I now leave them in a 60C oven ( only cos that's all I have) for 60 mins, minimum. 6. Place into xylene. Leave for 10 mins before mounting . A nice easy, reliable method, imho. NB: after cutting/mounting sections, leave in 60C oven for 2 hrs to ensure that they are "baked" on. Carl _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmjohnson34 <@t> hotmail.com Wed Aug 27 14:37:17 2008 From: jmjohnson34 <@t> hotmail.com (Jennifer Johnson) Date: Wed Aug 27 14:37:22 2008 Subject: [Histonet] Large funnel Message-ID: I bought a metal funnel at an Auto Parts store for formalin disposal and liked it so much I had a brainstorm that one of the oil funnels with the long hose would work great attached to the grease trap of my outdoor grill. I was so proud of myself. I got an empty disposable 2 gallon water bottle (like they use in the water cooler) from Lowe's and put the metal hosing down into the bottle. It worked great all summer, I was drawing up a patent when winter hit and all the grease congealed in the hose and overflowed into the cabinet of the grill. Oh well, back to the drawing board. I would rather be ingenious than ingenuous! _________________________________________________________________ Talk to your Yahoo! Friends via Windows Live Messenger. Find out how. http://www.windowslive.com/explore/messenger?ocid=TXT_TAGLM_WL_messenger_yahoo_082008 From jtrejo2 <@t> slu.edu Wed Aug 27 14:40:41 2008 From: jtrejo2 <@t> slu.edu (Julie Trejo) Date: Wed Aug 27 14:40:46 2008 Subject: [Histonet] Histotech to be pregnant with questions Message-ID: Hi. I'm a histotech about to start on getting pregnant to start our family, yet concerned on the birth defects that come from the xylene fumes. My great co-workers have decided to change processors etc to keep me away from chemicals, which is great for me, just still concerned about the fumes. I would only be embedding and cutting for the most part and we do have alot of automation ie: H&E stainer, Artisian (special stains), and immuno's (BondMax). Automation will keep my hands out of xylene which is wonderful, just worried. I've read some things about histotechs that were pregnant, some ok and some bad (over 20 years ago). Just haven't found out about the recent ones and how their child was. I asked one of our pathologists if it was okay to work in this enviorment but she thinks its okay. I've worked with alot of women whom had children but they never were working in histology when they were pregnant. Basically, I do enjoy my job and don't want to leave where I am, but worry about being pregnant in the histo lab. Is it okay to stay or should I get out or what can I do to stay without harming/risking my baby? I would love to hear from past pregnant mom's in histo lab and let me know if it worked etc. Thanks -- Julie Trejo, HT(ASCP)cm From gayle.callis <@t> bresnan.net Wed Aug 27 15:43:12 2008 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Wed Aug 27 15:43:17 2008 Subject: [Histonet] Re: mechanism of staining for Sanderson's with acid fuchsin PMMA embedded bone Message-ID: <002901c90885$85f37f90$6501a8c0@Sunney> Sanderson's bone stain is the same as Stevenels blue developed for PMMA bone work by Maniatopoulos C, Rodriguez A, Deporter DA, Melcher AH: An improved method for preparing histological sections of metallic implants. Internat J Oral & Maxillofacial Implants 1(1):31, 1987. The stain was originally developed as a parasite stain. Sanderson figured out an easier way to make up the staining solution than the original recipe, and then it was marketed by Surgipath with trademark. Her recipe is proprietary and stains with the same results as the Maniatopoulos method. We did the comparison in our lab and had identical results. Because the Stevenel's is such a royal pain to make in the lab, I suggest buying the Sanderson Bone stain. It is money well spent to avoid a long day of stain preparation. The chemistry of making this stain is interesting in that potassium permangante oxidizes methylene blue, forming a thick gooey ppt that takes a great deal of stirring and heating to get things into solution. The pH is very alkaline, somewhere in 9 or higher range and when using this stain. Continued heating of the solution causes the remaining methylene blue oxidize and the pH continues to increase. We found our home made Stevenels needed to be topped off frequently, and also filtered since a black ppt keeps forming. Conn's Biological stains Lillie, RD, revised by Stotz EH and Emmel,VM: H J Conns Biological Stains. pp 423-424, Ninth ed., Williams and Wilkins Co, Baltimore MD 1977 has explanation of what happens to the methylene blue when oxidized by KMNO4. The by- products of the methylene blue oxidation are toluidine blue, methylene violet, thionin, Azure A and other Azures along with residual methylene blue left in the solution. Some dyes are often found in formulations/recipes for PMMA embedded bone sections e.g. toluidine blue. Staining results are Osteoid - blue to intense blue-green; Muscle, connective tissue - blue to blue-green; Cartilage - blue and/or shades of violet to purple; Calcified cartilage - medium to dark purple Calcified bone is stained by acid fuchsin, and light green can also be used. Since PMMA is very hydrophobic, only low molecular weight dyes penetrate the plastic sufficiently in order to stain all the the described components. Acid fuchsin also has a low molecular weight. RW Horobin has a wonderful publication on the effects of staining on plastics. This paper alone is an education on how dyes work on plastic embedded tissues, including PMMA and GMA. Horobin RW. Staining plastic sections: a review of problems, explanations, and possible solutions. J Microscopy 131:173-186, 1982. Some important factors that affect staining of PMMA embedded bone are Low molecular weight dyes High temperature Alkaline pH These factors also affect how hydrophilic EM plastics and other methacrylate embedded tissues stain. It is well established that toluidine blue, in sodium borate buffer at pH 11 is a stain of choice for EM embedded tissues, and requires heating. Hopefully this will help in understanding the mechanism. I suggest accessing the publications for further information. Gayle M. Callis HTL/HT/MT(ASCP) From PMonfils <@t> Lifespan.org Wed Aug 27 16:26:19 2008 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Aug 27 16:26:24 2008 Subject: [Histonet] (para)formaldehyde In-Reply-To: <002801c9083b$5b9c6a80$97a5d680@mmkem12636> Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835C54@LSRIEXCH1.lsmaster.lifespan.org> 10% formalin and 4% paraformaldehyde are interchangeable for most purposes (in histology at least). However there are a couple of minor differences. First, commercial formaldehyde solution contains 37% to 38% formaldehyde. Therefore diluting it 1:9 results in a solution containing 3.7% to 3.8% formaldehyde, while a 4% solution of paraformaldehyde in water contains a full 4% formaldehyde. Secondly, commercial formaldehyde solution contains 10% to 15% methanol as a preservative. Therefore diluting it 1:9 results in a solution containing 1.0% to 1.5% methanol. This is not a problem for most histological applications, but it could be a problem in a procedure where sources of methylation have to be avoided. 4% paraformaldehyde solution contains no methanol - nothing but water and formaldehyde. From Carlos.Hurtado <@t> samc.com Wed Aug 27 17:36:47 2008 From: Carlos.Hurtado <@t> samc.com (Carlos Hurtado) Date: Wed Aug 27 17:37:06 2008 Subject: [Histonet] tissue floaters Message-ID: <48B57494.8B6C.0042.0@samc.com> Does anyone have any suggestions to eliminate/reduce the amount of floaters found in tissue blocks or paraffin baths? Is there any QC method to track the amount? Trinity Health MailGate made the following annotations --------------------------------------------------------------------- Confidentiality Note: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient(s) is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail and destroy the original message and all copies. --------------------------------------------------------------------- From amosbrooks <@t> gmail.com Wed Aug 27 18:36:02 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Aug 27 18:36:07 2008 Subject: [Histonet] Hematoxylin ... STILL!! Message-ID: <582736990808271636n30f3f703xfab18a11bbfdac75@mail.gmail.com> Hi, OK In the absence of hematoxylin, the substitutes can hold people over that run out of pre-made stuff. What are people doing for powder? Are there any non-hematoxylin substitutes for Weigerts Iron hematoxylin? How about Veerhoffs elastic stain? My order was placed with Sigma in April ... Back ordered 'till Aug 15th ... still waiting ... MONGO GETTING MAD ... GRR! Amos From detmar <@t> mshri.on.ca Wed Aug 27 18:50:49 2008 From: detmar <@t> mshri.on.ca (Jacqui Detmar) Date: Wed Aug 27 18:51:05 2008 Subject: [Histonet] (para)formaldehyde In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E03835C54@LSRIEXCH1.lsmaster.lifespan.org> References: <002801c9083b$5b9c6a80$97a5d680@mmkem12636> <4EBFF65383B74D49995298C4976D1D5E03835C54@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: I thought I heard something about 10% formalin causing more DNA/RNA breaks than 4% paraformaldehyde. Have I been having strange dreams again? The lowly post-doc , Jacqui Jacqui Detmar, Post-doctoral Fellow Samuel Lunenfeld Research Institute, Mount Sinai Hospital 25 Orde Street, room 6-1001 AJ, Toronto, ON, Canada M5T 3H7 phone: 416-586-4800 x5607 fax: 416-586-8588 email: detmar@mshri.on.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Wednesday, August 27, 2008 5:26 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (para)formaldehyde 10% formalin and 4% paraformaldehyde are interchangeable for most purposes (in histology at least). However there are a couple of minor differences. First, commercial formaldehyde solution contains 37% to 38% formaldehyde. Therefore diluting it 1:9 results in a solution containing 3.7% to 3.8% formaldehyde, while a 4% solution of paraformaldehyde in water contains a full 4% formaldehyde. Secondly, commercial formaldehyde solution contains 10% to 15% methanol as a preservative. Therefore diluting it 1:9 results in a solution containing 1.0% to 1.5% methanol. This is not a problem for most histological applications, but it could be a problem in a procedure where sources of methylation have to be avoided. 4% paraformaldehyde solution contains no methanol - nothing but water and formaldehyde. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From micro <@t> superlink.net Wed Aug 27 19:05:39 2008 From: micro <@t> superlink.net (Markus F. Meyenhofer) Date: Wed Aug 27 19:05:47 2008 Subject: [Histonet] Hematoxylin ... STILL!! References: <582736990808271636n30f3f703xfab18a11bbfdac75@mail.gmail.com> Message-ID: <086301c908a1$cedd3450$d3893cd1@DJ4VDH31> How much Hematoxylin (powder) do you need? Regards, Markus F. Meyenhofer Microscopy Labs Box 338 61 West Street Red Bank, NJ 07701 732 747 6228 micro@superlink.net ----- Original Message ----- From: "Amos Brooks" To: Sent: Wednesday, August 27, 2008 7:36 PM Subject: [Histonet] Hematoxylin ... STILL!! > Hi, > OK In the absence of hematoxylin, the substitutes can hold people over > that run out of pre-made stuff. What are people doing for powder? Are > there > any non-hematoxylin substitutes for Weigerts Iron hematoxylin? How about > Veerhoffs elastic stain? My order was placed with Sigma in April ... Back > ordered 'till Aug 15th ... still waiting ... > > MONGO GETTING MAD ... GRR! > > Amos > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tim.morken <@t> thermofisher.com Wed Aug 27 19:16:21 2008 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Wed Aug 27 19:16:51 2008 Subject: [Histonet] (para)formaldehyde (was: in situ question) Message-ID: <6BFF6D137DF6BC43B33891BA96E83B1901D00708@PGHCR-EXMB-VS-1.na.fshrnet.com> There are three reasons to use paraformaldehyde to make formalin: 1) Eliminate the methanol preservative of commercial formalin 2) customize the formaldehyde concentration 3) customize the buffer used. One of these three variables may affect your test so making your own allows you to control them. If you see a difference in the results when using two "types" of formalin, consider these variables. Tim Morken Technical Support Manager Lab Vision Products Anatomical Pathology Thermo Fisher Scientific Hello again! I don't have ANY scientific proof whatsoever of the difference between 10% formalin and 4% formaldehyde made from PFA. It's just something I think I have noticed in the lab, clearly enough to make me wonder. But you are right that the fixation protocols were never specifically controlled to allow real comparison, so maybe it indeed was some other factor. I grew in a developmental biology group and first learnt to use "PFA", and eventually started to wonder why some use it and some use formalin (often being the same person, for different applications!). Maybe I should really investigate it some day. With best regards, Mikael ----------------------------------------------------- Mikael Niku, PhD, university lecturer University of Helsinki, Division of Nutrition URL: mikael.nikunnakki.info - What do I think of western civilization? I think it would be a good idea! Gandhi ----------------------------------------------------- -----Original Message----- From: Tony Henwood [mailto:AnthonyH@chw.edu.au] Sent: 22. elokuuta 2008 2:17 To: Mikael Niku; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (para)formaldehyde (was: in situ question) Why would it be different? I am intrigued as to why IPX and ISH would reaxct differently in tissues fixed in either. Do you have any references? Or please write up your results and publish them (J Histotechnology or Biotechnic & Histochem are definitely worth considering). Often there will be differences but my experience indicates that it is often due to the fixation time. 10% buffered formalin is usually used at room temp, overnight at least, where as researchers for some reason often use 4% formaldehyde (made from polyformaldehyde) at 4oC for only a few hours at best. So often fixation occurs in the ethanols in processing. For valid comparisons fixation conditions must be standardised. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 From lpwenk <@t> sbcglobal.net Wed Aug 27 20:08:32 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed Aug 27 20:08:42 2008 Subject: [Histonet] Hematoxylin ... STILL!! In-Reply-To: <582736990808271636n30f3f703xfab18a11bbfdac75@mail.gmail.com> Message-ID: <002001c908aa$974ae420$0202a8c0@HPPav2> If the Weigert is for a Trichrome - skip it. The important part is muscle red, collagen blue or green. On most trichromes, the black of Weigert doesn't stick around anyway, and the nuclei are end up red. If you don't tell them, they would probably never notice the lack of Weigert in a trichrome. In place of VVG, how about an aldehyde fuchsin (using acetaldehyde instead of paraldehyde if making your own), or the resorcin fuchsin stain (usually available in kit form if you want it that way). Or the Orcein stain, also known as Pinkus. All of these stain fine and coarse elastin, while VVG only stains coarse elastin, so the pathologists just have to get used to seeing MORE elastin than they are used to, and that the elastin will be violet, blue/black or brown, not black. But hey, they have stained elastin. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Wednesday, August 27, 2008 7:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hematoxylin ... STILL!! Hi, OK In the absence of hematoxylin, the substitutes can hold people over that run out of pre-made stuff. What are people doing for powder? Are there any non-hematoxylin substitutes for Weigerts Iron hematoxylin? How about Veerhoffs elastic stain? My order was placed with Sigma in April ... Back ordered 'till Aug 15th ... still waiting ... MONGO GETTING MAD ... GRR! Amos _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Aug 28 07:40:14 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Aug 28 07:40:17 2008 Subject: [Histonet] tissue floaters In-Reply-To: <48B57494.8B6C.0042.0@samc.com> Message-ID: <222334.259.qm@web65709.mail.ac4.yahoo.com> Just good old fashion attention to detail and cleaning instruments and the forceps?heating? wells in the embedding centers. Eliminating not used floating tissue sections between blocks is also a must. Ren? J. --- On Wed, 8/27/08, Carlos Hurtado wrote: From: Carlos Hurtado Subject: [Histonet] tissue floaters To: histonet@lists.utsouthwestern.edu Date: Wednesday, August 27, 2008, 6:36 PM Does anyone have any suggestions to eliminate/reduce the amount of floaters found in tissue blocks or paraffin baths? Is there any QC method to track the amount? Trinity Health MailGate made the following annotations --------------------------------------------------------------------- Confidentiality Note: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient(s) is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail and destroy the original message and all copies. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Aug 28 07:50:26 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Aug 28 07:50:33 2008 Subject: [Histonet] (para)formaldehyde In-Reply-To: Message-ID: <333928.53661.qm@web65710.mail.ac4.yahoo.com> Probably, because the "masking" of DNA/mRNA is due to the cross linking action of methanal (formaldehyde) over the proteins that exist in their close proximity and both "formalin" and formaldehyde solution prepared from paraformaldehyde act in the same way (via methanal). Ren? J. --- On Wed, 8/27/08, Jacqui Detmar wrote: From: Jacqui Detmar Subject: RE: [Histonet] (para)formaldehyde To: "Monfils, Paul" , histonet@lists.utsouthwestern.edu Date: Wednesday, August 27, 2008, 7:50 PM I thought I heard something about 10% formalin causing more DNA/RNA breaks than 4% paraformaldehyde. Have I been having strange dreams again? The lowly post-doc , Jacqui Jacqui Detmar, Post-doctoral Fellow Samuel Lunenfeld Research Institute, Mount Sinai Hospital 25 Orde Street, room 6-1001 AJ, Toronto, ON, Canada M5T 3H7 phone: 416-586-4800 x5607 fax: 416-586-8588 email: detmar@mshri.on.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Wednesday, August 27, 2008 5:26 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (para)formaldehyde 10% formalin and 4% paraformaldehyde are interchangeable for most purposes (in histology at least). However there are a couple of minor differences. First, commercial formaldehyde solution contains 37% to 38% formaldehyde. Therefore diluting it 1:9 results in a solution containing 3.7% to 3.8% formaldehyde, while a 4% solution of paraformaldehyde in water contains a full 4% formaldehyde. Secondly, commercial formaldehyde solution contains 10% to 15% methanol as a preservative. Therefore diluting it 1:9 results in a solution containing 1.0% to 1.5% methanol. This is not a problem for most histological applications, but it could be a problem in a procedure where sources of methylation have to be avoided. 4% paraformaldehyde solution contains no methanol - nothing but water and formaldehyde. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CBark <@t> memorialcare.org Thu Aug 28 09:01:51 2008 From: CBark <@t> memorialcare.org (Christine Bark) Date: Thu Aug 28 09:02:13 2008 Subject: [Histonet] Dishwasher Message-ID: Does anyone know of any regulations against having a household dishwasher in the lab? Thanks so much, Christine Bark HT(ASCP) CM Lead Histotech, Pathology Saddleback Memorial Medical Center 949-452-3548 cbark@memorialcare.org ______________________________________________________________________________ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From rjbuesa <@t> yahoo.com Thu Aug 28 09:11:52 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Aug 28 09:11:56 2008 Subject: [Histonet] Dishwasher In-Reply-To: Message-ID: <36174.30639.qm@web65701.mail.ac4.yahoo.com> The "rule of thumb" now enforced by the CAP is that any and all pieces of equipment should be used as per the manufacturers' indications and as originally intended, and no dishwasher has been designed to be used in a lab setting, even if they can do a pretty good job in cleaning glassware. Ren? J. --- On Thu, 8/28/08, Christine Bark wrote: From: Christine Bark Subject: [Histonet] Dishwasher To: histonet@lists.utsouthwestern.edu Date: Thursday, August 28, 2008, 10:01 AM Does anyone know of any regulations against having a household dishwasher in the lab? Thanks so much, Christine Bark HT(ASCP) CM Lead Histotech, Pathology Saddleback Memorial Medical Center 949-452-3548 cbark@memorialcare.org ______________________________________________________________________________ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lchausse <@t> nmh.org Thu Aug 28 09:15:04 2008 From: Lchausse <@t> nmh.org (Chaussey, Leslie) Date: Thu Aug 28 09:15:45 2008 Subject: [Histonet] Dishwasher In-Reply-To: <36174.30639.qm@web65701.mail.ac4.yahoo.com> Message-ID: Several companies sell laboratory glassware washers though. Leslie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, August 28, 2008 9:12 AM To: histonet@lists.utsouthwestern.edu; Christine Bark Subject: Re: [Histonet] Dishwasher The "rule of thumb" now enforced by the CAP is that any and all pieces of equipment should be used as per the manufacturers' indications and as originally intended, and no dishwasher has been designed to be used in a lab setting, even if they can do a pretty good job in cleaning glassware. Ren? J. --- On Thu, 8/28/08, Christine Bark wrote: From: Christine Bark Subject: [Histonet] Dishwasher To: histonet@lists.utsouthwestern.edu Date: Thursday, August 28, 2008, 10:01 AM Does anyone know of any regulations against having a household dishwasher in the lab? Thanks so much, Christine Bark HT(ASCP) CM Lead Histotech, Pathology Saddleback Memorial Medical Center 949-452-3548 cbark@memorialcare.org ______________________________________________________________________________ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- This message and any included attachments are intended only for the addressee. The information contained in this message is confidential and may constitute proprietary or non-public information under international, federal, or state laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail. From carlos <@t> clubstaffing.com Thu Aug 28 09:16:22 2008 From: carlos <@t> clubstaffing.com (Carlos Stolk) Date: Thu Aug 28 09:16:27 2008 Subject: [Histonet] Dishwasher In-Reply-To: References: Message-ID: I think it is illegal in three states. Carlos Stolk Account Representative Georgia | New Mexico South Carolina| Texas Phone: 800-875-8999 ext. 258 Fax: 561-367-0884 carlos@clubstaffing.com Club Staffing, Inc. 5901 Broken Sound Pkwy, Ste 500 Boca Raton, FL 33487 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Christine Bark Sent: Thursday, August 28, 2008 10:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dishwasher Does anyone know of any regulations against having a household dishwasher in the lab? Thanks so much, Christine Bark HT(ASCP) CM Lead Histotech, Pathology Saddleback Memorial Medical Center 949-452-3548 cbark@memorialcare.org ________________________________________________________________________ ______ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From stema_cba <@t> yahoo.it Thu Aug 28 09:49:01 2008 From: stema_cba <@t> yahoo.it (Stefano Mantero) Date: Thu Aug 28 09:49:10 2008 Subject: [Histonet] Ottix Shaper Message-ID: <264760.21181.qm@web23107.mail.ird.yahoo.com> Hi, I am starting to use a Leica TP1020 Tissue Processor with Diapath reagents. How I can estimate the exhaustion of Ottix Shaper? I can measure the density? Thanks in advance Stefano __________________________________________________ Do You Yahoo!? Poco spazio e tanto spam? Yahoo! Mail ti protegge dallo spam e ti da tanto spazio gratuito per i tuoi file e i messaggi http://mail.yahoo.it From histoinfo <@t> comcast.net Thu Aug 28 12:16:27 2008 From: histoinfo <@t> comcast.net (histoinfo@comcast.net) Date: Thu Aug 28 12:16:31 2008 Subject: [Histonet] Thick Sections of Cornea Message-ID: <082820081716.9362.48B6DD6B00077A7D00002492221556707401000207019B9C0708@comcast.net> Good Morning Netters, A friend asked me if I had any experience with cutting and staining thick sections (30+ microns) of cornea for special stains for nerve?? My first question was how would you get sections that thick to stay on the slide? Second question is what would be the best stain for something like this and , would any of the staining times need to be lengthened due to the thickness of the sections? More questions I haven't thought of? Does anyone know how to do this? Would they be willing to share their procedure? Any help, however small would be a gret help. Many thanks, Jennier Saunders HT (ASCP) From sbreeden <@t> nmda.nmsu.edu Thu Aug 28 12:40:43 2008 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Aug 28 12:40:50 2008 Subject: [Histonet] Who? (OT!) Message-ID: <4D14F0FC9316DD41972D5F03C070908B017E6494@nmdamailsvr.nmda.ad.nmsu.edu> Okay, I just have to ask because I've missed several Friday Hours of Fuming in a row and I'm in the mood to stir up some trouble... Who is Ottis Shaper and why would I want to measure his density? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 From amber.mckenzie <@t> gastrodocs.net Thu Aug 28 13:29:38 2008 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Thu Aug 28 13:29:46 2008 Subject: [Histonet] ventana special stainer Message-ID: <03C921A1EAF7F541B16543F6EC6A4B3701E1C68D@giamail2.Gia.com> Does anyone use the ventana special stainer for the Steiner Stain? That's our primary stain and I have the hardest trouble getting my slides to look clean. I can decontaminate the instrument, level it, clean the slide carousel, check the tubing for clogs, open a new kit and it will still have background stain. I use the plain slides from Cardinal b/c the plus slides have way too much debris on the background. Any suggestions? From hana444 <@t> gmail.com Thu Aug 28 15:53:57 2008 From: hana444 <@t> gmail.com (Hana Peter) Date: Thu Aug 28 15:54:04 2008 Subject: [Histonet] Hematoxylin stability Message-ID: <48B71065.8020206@gmail.com> Hi, I recently came into possession of a small amount of hematoxylin powder and for the first time I'm going to make my own Harris hematoxylin and maybe some Mayer's too. As I have a limited amount of powder, I need to be extra careful with my supply. Does anyone have experience with "home" made hematoxylin and its stability? I found on the internet that the stability is up to 6 months. Is this really true? Thanks a lot! Hana Peter From mcauliff <@t> umdnj.edu Thu Aug 28 16:00:12 2008 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Aug 28 15:59:47 2008 Subject: [Histonet] Hematoxylin stability In-Reply-To: <48B71065.8020206@gmail.com> References: <48B71065.8020206@gmail.com> Message-ID: <48B711DC.9010009@umdnj.edu> I make Delafield's hematoxylin, it is stable for over one year. Geoff Hana Peter wrote: > Hi, > I recently came into possession of a small amount of hematoxylin powder > and for the first time I'm going to make my own Harris hematoxylin and > maybe some Mayer's too. > As I have a limited amount of powder, I need to be extra careful with my > supply. > Does anyone have experience with "home" made hematoxylin and its > stability? > I found on the internet that the stability is up to 6 months. Is this > really true? > Thanks a lot! > Hana Peter > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcauliff@umdnj.edu ********************************************** From rjbuesa <@t> yahoo.com Thu Aug 28 16:14:30 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Aug 28 16:14:33 2008 Subject: [Histonet] Hematoxylin stability In-Reply-To: <48B71065.8020206@gmail.com> Message-ID: <751921.31918.qm@web65711.mail.ac4.yahoo.com> In some occasions I have used hematoxylin powder Much Much older than 6 months and the solutions came perfect. Fear not to your powder! Ren? J. --- On Thu, 8/28/08, Hana Peter wrote: From: Hana Peter Subject: [Histonet] Hematoxylin stability To: histonet@lists.utsouthwestern.edu Date: Thursday, August 28, 2008, 4:53 PM Hi, I recently came into possession of a small amount of hematoxylin powder and for the first time I'm going to make my own Harris hematoxylin and maybe some Mayer's too. As I have a limited amount of powder, I need to be extra careful with my supply. Does anyone have experience with "home" made hematoxylin and its stability? I found on the internet that the stability is up to 6 months. Is this really true? Thanks a lot! Hana Peter _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Aug 28 16:15:52 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Aug 28 16:15:56 2008 Subject: [Histonet] ventana special stainer In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B3701E1C68D@giamail2.Gia.com> Message-ID: <634938.28523.qm@web65707.mail.ac4.yahoo.com> Call your Ventana rep to find out about his/her excuse! Ren? J. --- On Thu, 8/28/08, Amber McKenzie wrote: From: Amber McKenzie Subject: [Histonet] ventana special stainer To: histonet@lists.utsouthwestern.edu Date: Thursday, August 28, 2008, 2:29 PM Does anyone use the ventana special stainer for the Steiner Stain? That's our primary stain and I have the hardest trouble getting my slides to look clean. I can decontaminate the instrument, level it, clean the slide carousel, check the tubing for clogs, open a new kit and it will still have background stain. I use the plain slides from Cardinal b/c the plus slides have way too much debris on the background. Any suggestions? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Thu Aug 28 16:24:43 2008 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Thu Aug 28 16:24:47 2008 Subject: [Histonet] Hematoxylin stability In-Reply-To: <48B71065.8020206@gmail.com> References: <48B71065.8020206@gmail.com> Message-ID: <48B7179B.4090300@pathology.washington.edu> If you have a limited amount of powder, I would recommend making Mayer's as it is foolproof. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Hana Peter wrote: > Hi, > I recently came into possession of a small amount of hematoxylin powder > and for the first time I'm going to make my own Harris hematoxylin and > maybe some Mayer's too. > As I have a limited amount of powder, I need to be extra careful with my > supply. > Does anyone have experience with "home" made hematoxylin and its > stability? > I found on the internet that the stability is up to 6 months. Is this > really true? > Thanks a lot! > Hana Peter > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From we3smitty <@t> yahoo.com Thu Aug 28 18:13:13 2008 From: we3smitty <@t> yahoo.com (angela smith) Date: Thu Aug 28 18:13:19 2008 Subject: [Histonet] Leica Bond Message-ID: <687386.10014.qm@web62004.mail.re1.yahoo.com> Is anyone using the Leica Bond Max IHC stainer?? I will be evaluating it for several weeks and would like some feed back on those that are using it or have purchased it. Thank you, Angela From lpwenk <@t> sbcglobal.net Thu Aug 28 19:11:01 2008 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Aug 28 19:11:13 2008 Subject: [Histonet] Hematoxylin stability In-Reply-To: <48B71065.8020206@gmail.com> Message-ID: <001f01c9096b$b84d63c0$0202a8c0@HPPav2> If your Harris formulation involves mercuric oxide as the oxidizer, don't use it. Since mercury salts can NOT be disposed down the sink, how are you going to wash in running water after hematoxylin staining? And how would you dispose of the acid rinse that pulls out the excess hematoxylin? And again, how are you going to wash in running water after the acid rinse? There are Harris hematoxylin formulations using sodium iodate for the oxidizer. Now you don't have to worry about mercuric salts. Or use Mayer hematoxylin that also uses sodium iodate, no mercuric salts. Also, look at the amount of hematoxylin used in Harris vs. Mayer. Usually Harris uses about 5 g of hematoxylin vs. 1 g for Mayer. So if you only have a small amount of hematoxylin, use the Mayer formula, and use the stain progressively, not regressively. The traditional Mayer hematoxylin uses citric acid for acidification, but acetic acid is just as effective, and most labs have acetic acid readily available. Just another hint. We like the pH about 2.45. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hana Peter Sent: Thursday, August 28, 2008 4:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hematoxylin stability Hi, I recently came into possession of a small amount of hematoxylin powder and for the first time I'm going to make my own Harris hematoxylin and maybe some Mayer's too. As I have a limited amount of powder, I need to be extra careful with my supply. Does anyone have experience with "home" made hematoxylin and its stability? I found on the internet that the stability is up to 6 months. Is this really true? Thanks a lot! Hana Peter _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aakrasht <@t> yahoo.com Thu Aug 28 20:32:36 2008 From: aakrasht <@t> yahoo.com (Ali A. Krasht) Date: Thu Aug 28 20:32:41 2008 Subject: [Histonet] PCR NAIL FUNGUS TESTING Message-ID: <576862.2679.qm@web50912.mail.re2.yahoo.com> Hi there Any body knows?a laboratory?in the USA that does PCR? for nail fungus testing. We call all over but with no results. Lately Doctors are asking for PCR nail fungus testing and I would like to know if anyone does. Please help. Do you know what specific PCR machine would do?the fungus testing,?because Ive asked manufacturers and they want over $20000 help. Ali From amosbrooks <@t> gmail.com Thu Aug 28 20:41:14 2008 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Thu Aug 28 20:41:19 2008 Subject: [Histonet] Hematoxylin ... STILL!! Message-ID: <582736990808281841q4251f953qccc39e9d8b247553@mail.gmail.com> Peggy, Thanks for the advise. I'll look into these. I've avoided them in the past because they looked rather difficult to make up, especially trying to get a hold of some paraldehyde. It's good to know there's a substitute since I couldn't find anyone that would even sell it. Amos Message: 11 Date: Wed, 27 Aug 2008 21:08:32 -0400 From: "Lee & Peggy Wenk" Subject: RE: [Histonet] Hematoxylin ... STILL!! To: "'Amos Brooks'" , Message-ID: <002001c908aa$974ae420$0202a8c0@HPPav2> Content-Type: text/plain; charset="US-ASCII" If the Weigert is for a Trichrome - skip it. The important part is muscle red, collagen blue or green. On most trichromes, the black of Weigert doesn't stick around anyway, and the nuclei are end up red. If you don't tell them, they would probably never notice the lack of Weigert in a trichrome. In place of VVG, how about an aldehyde fuchsin (using acetaldehyde instead of paraldehyde if making your own), or the resorcin fuchsin stain (usually available in kit form if you want it that way). Or the Orcein stain, also known as Pinkus. All of these stain fine and coarse elastin, while VVG only stains coarse elastin, so the pathologists just have to get used to seeing MORE elastin than they are used to, and that the elastin will be violet, blue/black or brown, not black. But hey, they have stained elastin. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 From marktarango <@t> gmail.com Thu Aug 28 22:29:07 2008 From: marktarango <@t> gmail.com (Mark Tarango) Date: Thu Aug 28 22:29:13 2008 Subject: [Histonet] PCR NAIL FUNGUS TESTING In-Reply-To: <576862.2679.qm@web50912.mail.re2.yahoo.com> References: <576862.2679.qm@web50912.mail.re2.yahoo.com> Message-ID: <5b6eb13e0808282029k10a78da5odffc6048df5aa9e0@mail.gmail.com> I don't know of anyone who does testing on nail fungus this way, but I'd think anything with a thermal cycler would work. You'd have to design the primers and assay. On 8/28/08, Ali A. Krasht wrote: > Hi there > > Any body knows a laboratory in the USA that does PCR for nail fungus testing. We call all over but with no results. Lately Doctors are asking for PCR nail fungus testing and I would like to know if anyone does. Please help. > > Do you know what specific PCR machine would do the fungus testing, because Ive asked manufacturers and they want over $20000 help. > > Ali > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Cathy.Crumpton <@t> tuality.org Fri Aug 29 07:02:10 2008 From: Cathy.Crumpton <@t> tuality.org (Cathy.Crumpton@tuality.org) Date: Fri Aug 29 07:02:19 2008 Subject: [Histonet] Help please from Cerner Millennium users In-Reply-To: <20080828170245.2AD5B13216C5@tumbleweed.corp.tuality.net> References: <20080828170245.2AD5B13216C5@tumbleweed.corp.tuality.net> Message-ID: We are six weeks into go live with Cerner Mil Director (who could not be bothered to give much in build) has suddenly decided that the way Millennium splits u fluids and tissues is "unacceptable". She has real issues with bronch bx and washings being taken in the same procedure but getting separa our end the patholog referencing the other access other labs handled this issue?&n is making it out to be? The path into our build did not seem to have a probl us find our current solution. What have other From robinsoc <@t> mercyhealth.com Fri Aug 29 08:00:56 2008 From: robinsoc <@t> mercyhealth.com (Cynthia Robinson) Date: Fri Aug 29 08:01:18 2008 Subject: [Histonet] Help please from Cerner Millennium users In-Reply-To: References: <20080828170245.2AD5B13216C5@tumbleweed.corp.tuality.net> Message-ID: <48B7ACB8.59AC.00AF.0@mercyhealth.com> We have Cerner Millennium and we do things the way you explain with no problem. We have had discussions regarding the split of cytology and tissue and the way it looks on reports. We now use Cerner PowerChart on the clinical side so that has helped physicians with finding all the reports. Would you like to discuss options off this discussion thread about statistic capture, cytotech involvement, database report management I would be happy to chat with you? Cindi Robinson, HT(ASCP) MMC-Sioux City Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 712-279-2768 >>> 8/29/2008 7:02 AM >>> We are six weeks into go live with Cerner Mil= lennium and our Medical Director (who could not be bothered to give much in= put during the build) has suddenly decided that the way Millennium splits u= p our fluids and tissues is "unacceptable". She has real issues with bronch bx and washings being taken in the same procedure but getting separa= te SP and CY accession numbers. We have done what we could on our end= by keeping the specimens together when they are turned out to the patholog= ists and by making order comments on each case referencing the other access= ion number. My question is, how have other labs handled this issue?&n= bsp; Is this as big of a deal as she is making it out to be? The path= ologist who did give some input into our build did not seem to have a probl= em with this and helped us find our current solution. What have other= 's experiences been? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Aug 29 09:04:32 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 29 09:04:37 2008 Subject: [Histonet] PCR NAIL FUNGUS TESTING In-Reply-To: <576862.2679.qm@web50912.mail.re2.yahoo.com> Message-ID: <978231.39924.qm@web65709.mail.ac4.yahoo.com> And that idea of your pathologists where does it come from? Wish-full thinking or a concrete published information. If from the second source, ask them for details, it will be easier this way than trying to depend on the good will of somebody to guide you. Ren? J. --- On Thu, 8/28/08, Ali A. Krasht wrote: From: Ali A. Krasht Subject: [Histonet] PCR NAIL FUNGUS TESTING To: histonet@lists.utsouthwestern.edu Date: Thursday, August 28, 2008, 9:32 PM Hi there Any body knows?a laboratory?in the USA that does PCR? for nail fungus testing. We call all over but with no results. Lately Doctors are asking for PCR nail fungus testing and I would like to know if anyone does. Please help. Do you know what specific PCR machine would do?the fungus testing,?because Ive asked manufacturers and they want over $20000 help. Ali _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From making <@t> ufl.edu Fri Aug 29 09:14:59 2008 From: making <@t> ufl.edu (MKing) Date: Fri Aug 29 09:16:29 2008 Subject: [Histonet] (para)formaldehyde Message-ID: <48B80463.9000503@ufl.edu> The statement that 4% paraformaldehyde solution contains nothing but water and formaldehyde is simply not true: as has been discussed thoroughly and often on Histonet (see archives), formaldehyde in aqueous solution spontaneously decomposes. This is precisely why formalin solutions are used, with methanol 'parking' the chemical reaction. Without measurement one never knows exactly how much formaldehyde is really present in solutions made from paraformaldehyde, although freshly made solutions will have concentrations closest to 4%. It is highly improbable that 3.8 and 4.0% solutions would produce any detectable and reproducible differences on any histological procedure, although the methanol added to formalin might. Mike King UF Pharmacology & Therapeutics ----------------- Message: 5 Date: Wed, 27 Aug 2008 17:26:19 -0400 From: "Monfils, Paul" Subject: RE: [Histonet] (para)formaldehyde To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835C54@LSRIEXCH1.lsmaster.lifespan.org> Content-Type: text/plain; charset="iso-8859-1" 10% formalin and 4% paraformaldehyde are interchangeable for most purposes (in histology at least). However there are a couple of minor differences. First, commercial formaldehyde solution contains 37% to 38% formaldehyde. Therefore diluting it 1:9 results in a solution containing 3.7% to 3.8% formaldehyde, while a 4% solution of paraformaldehyde in water contains a full 4% formaldehyde. Secondly, commercial formaldehyde solution contains 10% to 15% methanol as a preservative. Therefore diluting it 1:9 results in a solution containing 1.0% to 1.5% methanol. This is not a problem for most histological applications, but it could be a problem in a procedure where sources of methylation have to be avoided. 4% paraformaldehyde solution contains no methanol - nothing but water and formaldehyde. From rjbuesa <@t> yahoo.com Fri Aug 29 09:27:46 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 29 09:27:57 2008 Subject: [Histonet] (para)formaldehyde In-Reply-To: <48B80463.9000503@ufl.edu> Message-ID: <400426.23933.qm@web65710.mail.ac4.yahoo.com> Just for detail sake, methanal (formaldehyde) does NOT decompose in water, it just reacts with it to become methanediol (methylene glycol) and the amount not reacting and remaining as formaldehyde has been estimated in about 0.1% of the total in a 4% formalin solution. The alcohol methanol is added NOT to prevent the formaldehyde hydration, but to as a stabilizer, to retard its polymerization, so it is very likely that between the moment a 4% formaldehyde solution is prepared using paraformaldehyde to the moment it starts to penetrate, bind and cross-link it will be very close to 4% Ren? J. --- On Fri, 8/29/08, MKing wrote: From: MKing Subject: [Histonet] (para)formaldehyde To: histonet@lists.utsouthwestern.edu Date: Friday, August 29, 2008, 10:14 AM The statement that 4% paraformaldehyde solution contains nothing but water and formaldehyde is simply not true: as has been discussed thoroughly and often on Histonet (see archives), formaldehyde in aqueous solution spontaneously decomposes. This is precisely why formalin solutions are used, with methanol 'parking' the chemical reaction. Without measurement one never knows exactly how much formaldehyde is really present in solutions made from paraformaldehyde, although freshly made solutions will have concentrations closest to 4%. It is highly improbable that 3.8 and 4.0% solutions would produce any detectable and reproducible differences on any histological procedure, although the methanol added to formalin might. Mike King UF Pharmacology & Therapeutics ----------------- Message: 5 Date: Wed, 27 Aug 2008 17:26:19 -0400 From: "Monfils, Paul" Subject: RE: [Histonet] (para)formaldehyde To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835C54@LSRIEXCH1.lsmaster.lifespan.org> Content-Type: text/plain; charset="iso-8859-1" 10% formalin and 4% paraformaldehyde are interchangeable for most purposes (in histology at least). However there are a couple of minor differences. First, commercial formaldehyde solution contains 37% to 38% formaldehyde. Therefore diluting it 1:9 results in a solution containing 3.7% to 3.8% formaldehyde, while a 4% solution of paraformaldehyde in water contains a full 4% formaldehyde. Secondly, commercial formaldehyde solution contains 10% to 15% methanol as a preservative. Therefore diluting it 1:9 results in a solution containing 1.0% to 1.5% methanol. This is not a problem for most histological applications, but it could be a problem in a procedure where sources of methylation have to be avoided. 4% paraformaldehyde solution contains no methanol - nothing but water and formaldehyde. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From froyer <@t> bitstream.net Fri Aug 29 09:39:46 2008 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Aug 29 09:39:58 2008 Subject: [Histonet] PCR NAIL FUNGUS TESTING In-Reply-To: <978231.39924.qm@web65709.mail.ac4.yahoo.com> References: <576862.2679.qm@web50912.mail.re2.yahoo.com> <978231.39924.qm@web65709.mail.ac4.yahoo.com> Message-ID: <003401c909e5$15b78800$7701a80a@Ford> Would this article be of help? http://jmm.sgmjournals.org/cgi/content/full/55/9/1211 Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL:? 612-839-1046 Phone:? 763-542-8725 Fax:? 763-546-4830 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Friday, August 29, 2008 9:05 AM To: histonet@lists.utsouthwestern.edu; Ali A. Krasht Subject: Re: [Histonet] PCR NAIL FUNGUS TESTING And that idea of your pathologists where does it come from? Wish-full thinking or a concrete published information. If from the second source, ask them for details, it will be easier this way than trying to depend on the good will of somebody to guide you. Ren? J. --- On Thu, 8/28/08, Ali A. Krasht wrote: From: Ali A. Krasht Subject: [Histonet] PCR NAIL FUNGUS TESTING To: histonet@lists.utsouthwestern.edu Date: Thursday, August 28, 2008, 9:32 PM Hi there Any body knows?a laboratory?in the USA that does PCR? for nail fungus testing. We call all over but with no results. Lately Doctors are asking for PCR nail fungus testing and I would like to know if anyone does. Please help. Do you know what specific PCR machine would do?the fungus testing,?because Ive asked manufacturers and they want over $20000 help. Ali _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amber.mckenzie <@t> gastrodocs.net Fri Aug 29 12:05:40 2008 From: amber.mckenzie <@t> gastrodocs.net (Amber McKenzie) Date: Fri Aug 29 12:05:48 2008 Subject: [Histonet] Ventana Stainer problem Message-ID: <03C921A1EAF7F541B16543F6EC6A4B3701E1C8C5@giamail2.Gia.com> Well, it's not the lot # of the steiner kits, b/c I just received a new shipment today and opened a new kit and I still have the same trashy background stain. I even tried Surigath's regular slides compared to Cardinals regular slides and they do the same thing. Any new ideas out there?? Amber McKenzie, B.S., HT (ASCP) 1405 N. State St., Suite 400 Jackson, MS 39202 (ph) 601-863-0388 (fax) 601-326-3532 From kmerriam2003 <@t> yahoo.com Fri Aug 29 12:06:55 2008 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Fri Aug 29 12:07:02 2008 Subject: [Histonet] DAB: old-fashioned style! Message-ID: <403287.27481.qm@web50312.mail.re2.yahoo.com> Hi All, It's been many moons since I had to make?DAB by hand and I have?lost my recipe.? Can anyone let me know how to make DAB from?powder and H2O2? Thanks, Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From burch007 <@t> mc.duke.edu Fri Aug 29 12:45:25 2008 From: burch007 <@t> mc.duke.edu (James L Burchette) Date: Fri Aug 29 12:45:32 2008 Subject: [Histonet] DAB: old-fashioned style! In-Reply-To: <403287.27481.qm@web50312.mail.re2.yahoo.com> Message-ID: Diaminobenzidine (DAB)* DAB Stock Concentrate, 20 mg/ml In 50 ml of room temperature 0.05 M Tris Buffer, dissolve 1.0 g 3,3' Diaminobenzidine powder (Sigma D5637). Filter and aliquot in desired amounts. Freeze for future use. Keeps indefinitely at -85 C Once thawed, use within 2-3 hours. *Note: DAB is a suspected carcinogen, use a hood, wear gloves, eye protection and mask when using DAB powder. Wear gloves when handling DAB stock liquid. Working DAB, 0.5 mg/ml Thaw DAB stock aliquot, add to the appropriate volume of room temperature 0.05 M Tris buffer and add the appropriate amount of 0.6% H2O2 for the desired total volume of working DAB DAB Stock Volumes DAB Concentration add 0.6% H2O2 QS with Tris to final volume of: 0.5 ml = 10 mg + 0.3 ml 20 ml 1.25 ml = 25 mg + 0.75 ml 50 ml 2.5 ml = 50 mg + 1.5 ml 100 ml Reaction time is usually 3-5 minutes. 0.05M Tris buffer, pH 7.5-7.6 1.39 g Trizma base 6.06 g Trizma HCl QS to 1000 ml with D H20 0.6% Hydrogen Peroxide To 98 ml of DH2O add 2 ml of 30% H2O2. Wear gloves and eye protection when using 30% H2O2. Use as needed. Has a 4 month shelf life. Store at 4oC. Imidazole Tris Buffer, 0.05M Used for DAB enhancement 1.39 g Trizma base 6.06 g Trizma HCl 3.4 g Imidazole, FW 68.08 Mix in 950 ml, adjust pH to 7.6, QS to 1000 ml Add your above stock DAB aliquot and H202 to this buffer for the desired final volume if you like an enhanced DAB reaction. You can also use 1% copper sulfate as an enhancement for 15-30 seconds after rinsing the DAB from your slides. Add 200 ul of 1% cobalt chloride to 50 ml of working DAB solution for a blue DAB reaction. I have never tried a post DAB enhancement with cobalt chloride, but I think it should work. JB Jim Burchette, HT(ASCP) QIHC "A simple histotech from a little country hospital in North Carolina" Kim Merriam Sent by: histonet-bounces@lists.utsouthwestern.edu 08/29/2008 01:08 PM To Histonet cc Subject [Histonet] DAB: old-fashioned style! Hi All, It's been many moons since I had to make DAB by hand and I have lost my recipe. Can anyone let me know how to make DAB from powder and H2O2? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Aug 29 13:10:41 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 29 13:10:44 2008 Subject: [Histonet] Ventana Stainer problem In-Reply-To: <03C921A1EAF7F541B16543F6EC6A4B3701E1C8C5@giamail2.Gia.com> Message-ID: <484833.51200.qm@web65707.mail.ac4.yahoo.com> I believe somebody advised to get an Artisan autostainer. I second that suggestion. Ren? J. --- On Fri, 8/29/08, Amber McKenzie wrote: From: Amber McKenzie Subject: [Histonet] Ventana Stainer problem To: histonet@lists.utsouthwestern.edu Date: Friday, August 29, 2008, 1:05 PM Well, it's not the lot # of the steiner kits, b/c I just received a new shipment today and opened a new kit and I still have the same trashy background stain. I even tried Surigath's regular slides compared to Cardinals regular slides and they do the same thing. Any new ideas out there?? Amber McKenzie, B.S., HT (ASCP) 1405 N. State St., Suite 400 Jackson, MS 39202 (ph) 601-863-0388 (fax) 601-326-3532 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Aug 29 13:16:54 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 29 13:16:59 2008 Subject: [Histonet] DAB: old-fashioned style! In-Reply-To: <403287.27481.qm@web50312.mail.re2.yahoo.com> Message-ID: <534227.85262.qm@web65712.mail.ac4.yahoo.com> 6 mg of DAB dissolved in 15 mL of PBS adding?200 ?L?of 3% hydrogen peroxide. First dissolve with agitation half of the DAB, then add the rest of the DAB and at the end add the hydrogen peroxide. The hydrogen peroxide will end at a concentration of 0.04% There you have it! Ren? J. --- On Fri, 8/29/08, Kim Merriam wrote: From: Kim Merriam Subject: [Histonet] DAB: old-fashioned style! To: "Histonet" Date: Friday, August 29, 2008, 1:06 PM Hi All, It's been many moons since I had to make?DAB by hand and I have?lost my recipe.? Can anyone let me know how to make DAB from?powder and H2O2? Thanks, Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maxim_71 <@t> mail.ru Fri Aug 29 13:33:27 2008 From: Maxim_71 <@t> mail.ru (Maxim_71@mail.ru) Date: Fri Aug 29 13:34:52 2008 Subject: [Histonet] Hematoxylin stability Message-ID: <48477087.20080829223327@mail.ru> We make hematoxylin Lillie from powder, which 2-3 years old. It works great for all specimens. Process of the preparation takes approximately 20-25 minutes. 1 L of solution is enough for 2 weeks, because level of reagent will down. We stains all our slides manually (up to 8000 slides or more per month). Our pathologisits and techs are happy with this formula. We used Lillie formula over 8 years. Just remember, that solution with distilled water only will be quickly vaporized than formula with add 30% glycerine. Stability of this solution is 1 year. One bottle stained fine after one year of the standing. We did not try to leave solution for greater period. Regards, Maxim Peshkov Russia, Taganrog. mailto:Maxim_71@mail.ru From JPCOLEMA <@t> sentara.com Fri Aug 29 14:04:54 2008 From: JPCOLEMA <@t> sentara.com (JOHN P COLEMAN) Date: Fri Aug 29 14:05:08 2008 Subject: [Histonet] Hematoxylin In-Reply-To: <20080829170828.55FAC19AAC6D@spamnet2.sentara.com> References: <20080829170828.55FAC19AAC6D@spamnet2.sentara.com> Message-ID: 1) I made a Harris modification and it was stable for over one year if ripened naturally, with less KMNO4. Best slides ever. 2) We are considering converting to Bond in our IHC lab from 7 Biogenex i6000 instruments. We've been using one for over a year now, and are looking forward to the transition. There are significant workflow and quality improvements. If you want detail I have it. **************************************** John P.J. Coleman HT(ASCP)QIHC Clinical Specialist Anatomic Pathology Sentara Laboratory Services Cell/Voicemail(757)335-2159 pager: (757)456-6695 office:-(757)388-3295 From McMahan <@t> vision.wustl.edu Fri Aug 29 14:07:25 2008 From: McMahan <@t> vision.wustl.edu (McMahan, Belinda) Date: Fri Aug 29 14:07:32 2008 Subject: [Histonet] Paula Pierce Message-ID: <1C5A23F7B48786408C6636410C8675323A4E00@EX04.wusm-pcf.wustl.edu> Hello, If she is on Histonet, would Paula Pierce please contact me off-line at mcmahan@vision.wustl.edu? Thank you, Belinda From ttroyer <@t> petersonlab.com Fri Aug 29 14:08:07 2008 From: ttroyer <@t> petersonlab.com (Travis Troyer) Date: Fri Aug 29 14:08:20 2008 Subject: [Histonet] Fixation and weekend processing Message-ID: <026601c90a0a$927bfe00$6e01010a@Peterson.local> I have a question regarding tissue processing. We do routine processing of tissue where the surgery has been done the same day. We have recently received a request to do autopsy blocks and a few of the blocks appear to be underprocessed and fairly soft appearing. I was wondering if we need to change the times in our processor for this and if so how much. Our current processing schedule is used for biopsies as well as larger specimens. Thanks, Travis Troyer Peterson Laboratory Services From rjbuesa <@t> yahoo.com Fri Aug 29 14:40:23 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 29 14:40:28 2008 Subject: [Histonet] Fixation and weekend processing In-Reply-To: <026601c90a0a$927bfe00$6e01010a@Peterson.local> Message-ID: <847999.45564.qm@web65715.mail.ac4.yahoo.com> You should change your fixation schedules rather that your tissue processing schedules. That could be a sign of incompletely fixed tissues. Ren? J. --- On Fri, 8/29/08, Travis Troyer wrote: From: Travis Troyer Subject: [Histonet] Fixation and weekend processing To: histonet@lists.utsouthwestern.edu Date: Friday, August 29, 2008, 3:08 PM I have a question regarding tissue processing. We do routine processing of tissue where the surgery has been done the same day. We have recently received a request to do autopsy blocks and a few of the blocks appear to be underprocessed and fairly soft appearing. I was wondering if we need to change the times in our processor for this and if so how much. Our current processing schedule is used for biopsies as well as larger specimens. Thanks, Travis Troyer Peterson Laboratory Services _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tissuetech <@t> juno.com Fri Aug 29 14:55:58 2008 From: tissuetech <@t> juno.com (tissuetech@juno.com) Date: Fri Aug 29 14:57:45 2008 Subject: [Histonet] Fixation and weekend processing Message-ID: <20080829.145558.12490.0@webmail10.vgs.untd.com> Travis Anytime you have autopsy you must not only change fixation time but also processing time. Small and large tissue will fix and process in much less time. My lab does autopsy on a regular schedule. We do small tissue in just under 5 hours, Large tissue is 10 hours, Autopsy is just over 18 hours. We have worked out these schedules over the past 5 years and this is the best for our lab, we do not have under or over processing. Hope that this helps. Fred Siena - Tissue Techniques - Dallas, Texas ____________________________________________________________ Click here to find the satellite television package that meets your needs. http://thirdpartyoffers.juno.com/TGL2141/fc/Ioyw6i3mzvzdAsU9y2Lm0HXJJY72rbtUTFRMHoGK5iDuKuZRtaPNbb/ From tissuetech <@t> juno.com Fri Aug 29 14:59:57 2008 From: tissuetech <@t> juno.com (tissuetech@juno.com) Date: Fri Aug 29 15:00:55 2008 Subject: [Histonet] Ventana Stainer problem Message-ID: <20080829.145957.12490.1@webmail10.vgs.untd.com> Amber You might contact Ventana on this issue, I have all ventana instrumentation (Immuno and Special Stainer's) for the past 4 years and have no problems at all. Fred Siena - Tissue Techniques - Dallas, Texas ____________________________________________________________ Save on Emergency Alert Systems. Click here. http://thirdpartyoffers.juno.com/TGL2141/fc/Ioyw6i3mXtZuPxoiXioibEzkYHAXtPJ6bevijVYtHoiLHDI8NqWNKx/ From lwright1 <@t> uab.edu Fri Aug 29 15:01:41 2008 From: lwright1 <@t> uab.edu (Laura E Wright) Date: Fri Aug 29 15:01:54 2008 Subject: [Histonet] isopropyl Message-ID: Hi, all! This is probably a very simple question, but can anyone tell me why isopropyl hardens tissues less than ethanol? I'm debating about which alcohol to use to process some invertebrate marine animal tissues. I've had problems with these tissues being to hard in the past. Any help is greatly appreciated! L. Wright Birmingham, AL From tkngflght <@t> yahoo.com Fri Aug 29 15:12:29 2008 From: tkngflght <@t> yahoo.com (Cheryl) Date: Fri Aug 29 15:12:32 2008 Subject: [Histonet] Seeking a temp tech for Houston In-Reply-To: <20080829.145558.12490.0@webmail10.vgs.untd.com> Message-ID: <436712.12348.qm@web50908.mail.re2.yahoo.com> Hi All! We're seeking a temp tech for a stand alone/work alone histo position for at least two months--possibly an opportunity for permanent if it is a superb fit. I pick up the phone in the evening and on weekends--all inquiries are welcome!? Cheryl? Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing Health Care Professionals - One GREAT fit at a time. 800.756.3309 From RSRICHMOND <@t> aol.com Fri Aug 29 15:29:32 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Fri Aug 29 15:29:37 2008 Subject: [Histonet] Re: Hematoxylin stability Message-ID: The name associated with an alum hematoxylin usually corresponds to the oxidant employed. You can find citations of the original publications in Lillie 3rd ed. or in Bolles-Lee's The Microtomist's Vade-Mecum if you're fortunate enough to get your hands on a copy of it. Harris hematoxylin was oxidized with mercuric oxide. You really can't handle mercury in a present day hospital laboratory in the USA. Dr. Harris's name should not be invoked if you aren't oxidizing with HgO. Delafield's hematoxylin has no added oxidant - you put the bottle under the counter with a loose stopper and let the ambient oxygen oxidize it. (Phosphotungstic acid hematoxylin - PTAH, if anyone remembers that - was usually air-oxidized.) Mayer hematoxylins are oxidized with sodium iodate, favored because the oxidation occurs instantly at room temperature. The amount of iodate should be great enough to produce an immediately usable stain, but small enough to leave much of the hematoxylin unoxidized in order to improve the shelf life of the mixture. - The Gill hematoxylins are Mayer hematoxylin formulations with the molar concentrations of hematoxylin and iodate rationally calculated, as set out by Baker in his Cytologic Technique. I think that commercial hematoxylins today are usually oxidized by bubbling oxygen through them, but I'm not sure of that. Other oxidants such as permanganate have been employed on occasion. Acid, usually acetic, is added in a quantity determined by one's desire to have a progressive (not further differentiated) or regressive (differentiated in a subsequent acid bath) stain. The wise pathologist leaves this choice to the histotechnologist. I do not want a regressive step in my frozen section stain sequence, however. A preservative is usually added - ethanol, ethylene glycol, glycerol, or several others. Chloral hydrate and paraldehyde, which are controlled substances (like sodium barbital, they are abusable sedatives) were formerly used as preservatives, but they're not worth the administrative hassle of keeping a controlled substance in the laboratory. Bob Richmond Samurai Pathologist and long-ago occasional hematoxylin brewer Knoxville TN From rjbuesa <@t> yahoo.com Fri Aug 29 15:31:38 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 29 15:31:45 2008 Subject: [Histonet] isopropyl In-Reply-To: Message-ID: <547189.54197.qm@web65703.mail.ac4.yahoo.com> It is an alcohol with three carbons, with a higher molecular weight (30% more) and with the alcohol group in the center carbon, slightly less miscible in water so less hydrophylic (dehydrates more slowly), but regardless of all this, if it works better for you, use it. Ren? J. --- On Fri, 8/29/08, Laura E Wright wrote: From: Laura E Wright Subject: [Histonet] isopropyl To: histonet@lists.utsouthwestern.edu Date: Friday, August 29, 2008, 4:01 PM Hi, all! This is probably a very simple question, but can anyone tell me why isopropyl hardens tissues less than ethanol? I'm debating about which alcohol to use to process some invertebrate marine animal tissues. I've had problems with these tissues being to hard in the past. Any help is greatly appreciated! L. Wright Birmingham, AL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Fri Aug 29 15:57:57 2008 From: RSRICHMOND <@t> aol.com (Robert Richmond) Date: Fri Aug 29 15:58:04 2008 Subject: [Histonet] Re: PCR nail fungus testing Message-ID: Ali A. Krasht (where?) asks if anybody >>knows a laboratory in the USA that does PCR for nail fungus testing. We call all over but with no results. Lately Doctors are asking for PCR nail fungus testing and I would like to know if anyone does. - Do you know what specific PCR machine would do the fungus testing, because I've asked manufacturers and they want over $20000 help.<< I Googled this problem, and could not find such a test offered by two of the largest commercial labs, LabCor and ARUP. The test may be commercially available in Europe or in India, according to what I read, but I suspect it's not ready for routine use. Both polymerase chain reaction (PCR) and restriction fragment length polymorphism (RLFP) methods exist. I found some European references to a test called Onychodiag. As we've discussed on this list before, third parties are reluctant to pay for systemic treatment of nail fungus infections without a tissue diagnosis. PCR might have greater sensitivity than conventional staining, but contamination would be a very serious issue, as would what fungal species are to be considered definite etiologic agents. It would be totally impractical to set this test up in your own laboratory. Bob Richmond Samurai Pathologist Knoxville TN From alexandra.meinl <@t> gmail.com Fri Aug 29 16:06:49 2008 From: alexandra.meinl <@t> gmail.com (Alexandra Meinl) Date: Fri Aug 29 16:06:56 2008 Subject: [Histonet] isopropyl In-Reply-To: <547189.54197.qm@web65703.mail.ac4.yahoo.com> References: <547189.54197.qm@web65703.mail.ac4.yahoo.com> Message-ID: Hello, Do you prefer isopropyl alcohol over ethanol? I'm also thinking about using isopropyl for our specimens. Isopropyl dehydrates more slowly, but how long is "more" when compared to ethanol? Are there any limitations (e.g. are there stainings that can't be done on isopropyl dehydrated tissue)? Thanks, Alex 2008/8/29 Rene J Buesa > It is an alcohol with three carbons, with a higher molecular weight (30% > more) and with the alcohol group in the center carbon, slightly less > miscible in water so less hydrophylic (dehydrates more slowly), but > regardless of all this, if it works better for you, use it. > Ren? J. > > From gmartin <@t> marshallmedical.org Fri Aug 29 18:24:40 2008 From: gmartin <@t> marshallmedical.org (Martin, Gary) Date: Fri Aug 29 18:24:49 2008 Subject: [Histonet] Help please from Cerner Millennium users In-Reply-To: References: <20080828170245.2AD5B13216C5@tumbleweed.corp.tuality.net> Message-ID: <6ED9D4252F278841A0593D3D788AF24C032CEC62@mailsvr.MARSHMED.local> We separate out cytology and surgical with a different prefix and there are no problems. As a matter of fact it is easier to collect data for various reports. I'm not sure but it may matter when it comes to billing. I think I remember our billing person commenting that there were denials when the two are bunched together. Gary -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cathy.Crumpton@tuality.org Sent: Friday, August 29, 2008 5:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help please from Cerner Millennium users We are six weeks into go live with Cerner Mil=ennium and our Medical Director (who could not be bothered to give much in=ut during the build) has suddenly decided that the way Millennium splits u= our fluids and tissues is "unacceptable". She has real issues with bronch bx and washings being taken in the same procedure but getting separa=e SP and CY accession numbers. We have done what we could on our end=y keeping the specimens together when they are turned out to the patholog=ists and by making order comments on each case referencing the other access=on number. My question is, how have other labs handled this issue?&n=sp; Is this as big of a deal as she is making it out to be? The path=logist who did give some input into our build did not seem to have a probl=m with this and helped us find our current solution. What have other=s experiences been? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Sat Aug 30 10:21:20 2008 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Aug 30 10:21:24 2008 Subject: [Histonet] isopropyl In-Reply-To: Message-ID: <276606.66021.qm@web65705.mail.ac4.yahoo.com> Perhaps the word "slowly" to characterize how isopropyl dehydrates is an "unfortunate" one, it would be "more gently" but the overall speed is the same. Some instruments and technologies, like the used in the Peloris tissue processor, rest on the sole use of isopropyl. Dehydration has no noticeable?impact in any staining. Ren? J. --- On Fri, 8/29/08, Alexandra Meinl wrote: From: Alexandra Meinl Subject: Re: [Histonet] isopropyl To: histonet@lists.utsouthwestern.edu Date: Friday, August 29, 2008, 5:06 PM Hello, Do you prefer isopropyl alcohol over ethanol? I'm also thinking about using isopropyl for our specimens. Isopropyl dehydrates more slowly, but how long is "more" when compared to ethanol? Are there any limitations (e.g. are there stainings that can't be done on isopropyl dehydrated tissue)? Thanks, Alex 2008/8/29 Rene J Buesa > It is an alcohol with three carbons, with a higher molecular weight (30% > more) and with the alcohol group in the center carbon, slightly less > miscible in water so less hydrophylic (dehydrates more slowly), but > regardless of all this, if it works better for you, use it. > Ren? J. > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maxim_71 <@t> mail.ru Sat Aug 30 14:10:33 2008 From: Maxim_71 <@t> mail.ru (Maxim_71@mail.ru) Date: Sat Aug 30 14:13:31 2008 Subject: [Histonet] isopropyl Message-ID: <1485290308.20080830231033@mail.ru> Alexandra: I used isopropanol as dehydratant in the same time as ethanol. In my opinion isopropyl alcohol is more better than ethanol, especially for bone, cartillage, uterus, mammary gland and other difficult specimens. Regards, Maxim Peshkov Russia, Taganrog. From dw18 <@t> uchicago.edu Sat Aug 30 15:04:46 2008 From: dw18 <@t> uchicago.edu (David A. Wright) Date: Sat Aug 30 15:04:55 2008 Subject: [Histonet] Re: dissolving DAB Message-ID: <20080830150446.BDR35973@m4500-03.uchicago.edu> Hi folks I'm at home without my notes, but I remember John Kiernan saying somewhere (Histonet, the Book?) that DAB dissolves better in pure water. Since then that's what I've done - dissolve in deionized water at 1mg/ml, vortex thoroughly, then bring up to rxn volume in Tris, pH 7.6. Works a treat! I stain thick (40um) sections, so I also like to preincubate in my DAB solution plus any intensifiers for 8-10 min to allow them to penetrate, then start the reaction by adding the peroxide later - it's a tiny molecule and gets everywhere very quickly. -david -- David A. Wright, PhD Section of Neurosurgery University of Chicago ================ ORIGINAL POST: Histonet Digest, Vol 57, Issue 41: Message: 2 Date: Fri, 29 Aug 2008 10:06:55 -0700 (PDT) From: Kim Merriam Subject: [Histonet] DAB: old-fashioned style! To: Histonet Hi All, It's been many moons since I had to make DAB by hand and I have lost my recipe. Can anyone let me know how to make DAB from powder and H2O2? Thanks, Kim ?Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From AnthonyH <@t> chw.edu.au Sun Aug 31 18:26:51 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Aug 31 18:27:08 2008 Subject: [Histonet] (para)formaldehyde In-Reply-To: <48B80463.9000503@ufl.edu> Message-ID: I agree Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MKing Sent: Saturday, 30 August 2008 12:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (para)formaldehyde The statement that 4% paraformaldehyde solution contains nothing but water and formaldehyde is simply not true: as has been discussed thoroughly and often on Histonet (see archives), formaldehyde in aqueous solution spontaneously decomposes. This is precisely why formalin solutions are used, with methanol 'parking' the chemical reaction. Without measurement one never knows exactly how much formaldehyde is really present in solutions made from paraformaldehyde, although freshly made solutions will have concentrations closest to 4%. It is highly improbable that 3.8 and 4.0% solutions would produce any detectable and reproducible differences on any histological procedure, although the methanol added to formalin might. Mike King UF Pharmacology & Therapeutics ----------------- Message: 5 Date: Wed, 27 Aug 2008 17:26:19 -0400 From: "Monfils, Paul" Subject: RE: [Histonet] (para)formaldehyde To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835C54@LSRIEXCH1.lsmaster.lifespan.or g> Content-Type: text/plain; charset="iso-8859-1" 10% formalin and 4% paraformaldehyde are interchangeable for most purposes (in histology at least). However there are a couple of minor differences. First, commercial formaldehyde solution contains 37% to 38% formaldehyde. Therefore diluting it 1:9 results in a solution containing 3.7% to 3.8% formaldehyde, while a 4% solution of paraformaldehyde in water contains a full 4% formaldehyde. Secondly, commercial formaldehyde solution contains 10% to 15% methanol as a preservative. Therefore diluting it 1:9 results in a solution containing 1.0% to 1.5% methanol. This is not a problem for most histological applications, but it could be a problem in a procedure where sources of methylation have to be avoided. 4% paraformaldehyde solution contains no methanol - nothing but water and formaldehyde. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Sun Aug 31 18:32:17 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Aug 31 18:32:26 2008 Subject: [Histonet] (para)formaldehyde In-Reply-To: <400426.23933.qm@web65710.mail.ac4.yahoo.com> Message-ID: AND I agree. Its now getting more precise in the Science. I would suggest that the NSH put together a committee to determine policy on the nomenclature of formalin solutions so that we can call the lemon the lemon and not the "Sprite" or "Solo" that we seem to have found ourselves in. Hopefully we can then agree on what we are going to call the stuff. There is definitely enough publications out there to start from. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Saturday, 30 August 2008 12:28 AM To: histonet@lists.utsouthwestern.edu; MKing Subject: Re: [Histonet] (para)formaldehyde Just for detail sake, methanal (formaldehyde) does NOT decompose in water, it just reacts with it to become methanediol (methylene glycol) and the amount not reacting and remaining as formaldehyde has been estimated in about 0.1% of the total in a 4% formalin solution. The alcohol methanol is added NOT to prevent the formaldehyde hydration, but to as a stabilizer, to retard its polymerization, so it is very likely that between the moment a 4% formaldehyde solution is prepared using paraformaldehyde to the moment it starts to penetrate, bind and cross-link it will be very close to 4% Ren? J. --- On Fri, 8/29/08, MKing wrote: From: MKing Subject: [Histonet] (para)formaldehyde To: histonet@lists.utsouthwestern.edu Date: Friday, August 29, 2008, 10:14 AM The statement that 4% paraformaldehyde solution contains nothing but water and formaldehyde is simply not true: as has been discussed thoroughly and often on Histonet (see archives), formaldehyde in aqueous solution spontaneously decomposes. This is precisely why formalin solutions are used, with methanol 'parking' the chemical reaction. Without measurement one never knows exactly how much formaldehyde is really present in solutions made from paraformaldehyde, although freshly made solutions will have concentrations closest to 4%. It is highly improbable that 3.8 and 4.0% solutions would produce any detectable and reproducible differences on any histological procedure, although the methanol added to formalin might. Mike King UF Pharmacology & Therapeutics ----------------- Message: 5 Date: Wed, 27 Aug 2008 17:26:19 -0400 From: "Monfils, Paul" Subject: RE: [Histonet] (para)formaldehyde To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E03835C54@LSRIEXCH1.lsmaster.lifespan.org> Content-Type: text/plain; charset="iso-8859-1" 10% formalin and 4% paraformaldehyde are interchangeable for most purposes (in histology at least). However there are a couple of minor differences. First, commercial formaldehyde solution contains 37% to 38% formaldehyde. Therefore diluting it 1:9 results in a solution containing 3.7% to 3.8% formaldehyde, while a 4% solution of paraformaldehyde in water contains a full 4% formaldehyde. Secondly, commercial formaldehyde solution contains 10% to 15% methanol as a preservative. Therefore diluting it 1:9 results in a solution containing 1.0% to 1.5% methanol. This is not a problem for most histological applications, but it could be a problem in a procedure where sources of methylation have to be avoided. 4% paraformaldehyde solution contains no methanol - nothing but water and formaldehyde. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Sun Aug 31 18:35:08 2008 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Aug 31 18:35:17 2008 Subject: [Histonet] DAB: old-fashioned style! In-Reply-To: <403287.27481.qm@web50312.mail.re2.yahoo.com> Message-ID: Try this DAB Reagent: 1. Take 40ml Tris Buffer-Wash and add 6mg of DAB (3,3, Diaminobenzidine Tetrahydrochloride Grade II (Sigma D5637)), mix. 2. Add 50?l of hydrogen peroxide, mix. 3. The solution is ready to use. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Saturday, 30 August 2008 3:07 AM To: Histonet Subject: [Histonet] DAB: old-fashioned style! Hi All, It's been many moons since I had to make DAB by hand and I have lost my recipe. Can anyone let me know how to make DAB from powder and H2O2? Thanks, Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. **********************************************************************